LOCUS       DACCSK010000001        74937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCSK010000001 DACCSK010000000
VERSION     DACCSK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.001"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(29..376)
                     /gene="rbfA"
                     /locus_tag="IVP39_000001"
     CDS             complement(29..376)
                     /gene="rbfA"
                     /locus_tag="IVP39_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7585112.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(403..2709)
                     /gene="infB"
                     /locus_tag="IVP39_000002"
     CDS             complement(403..2709)
                     /gene="infB"
                     /locus_tag="IVP39_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7585113.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2722..3033)
                     /locus_tag="IVP39_000003"
     CDS             complement(2722..3033)
                     /locus_tag="IVP39_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7585114.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3030..3323)
                     /locus_tag="IVP39_000004"
     CDS             complement(3030..3323)
                     /locus_tag="IVP39_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7585115.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3345..4520)
                     /gene="nusA"
                     /locus_tag="IVP39_000005"
     CDS             complement(3345..4520)
                     /gene="nusA"
                     /locus_tag="IVP39_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7585116.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4543..5016)
                     /gene="rimP"
                     /locus_tag="IVP39_000006"
     CDS             complement(4543..5016)
                     /gene="rimP"
                     /locus_tag="IVP39_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7585117.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5161..9513)
                     /locus_tag="IVP39_000007"
     CDS             complement(5161..9513)
                     /locus_tag="IVP39_000007"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7585118.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9725..11434)
                     /locus_tag="IVP39_000008"
     CDS             complement(9725..11434)
                     /locus_tag="IVP39_000008"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7585119.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11502..12770)
                     /gene="rseP"
                     /locus_tag="IVP39_000009"
     CDS             complement(11502..12770)
                     /gene="rseP"
                     /locus_tag="IVP39_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7585120.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12931..13731)
                     /locus_tag="IVP39_000010"
     CDS             complement(12931..13731)
                     /locus_tag="IVP39_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7585121.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13728..14540)
                     /locus_tag="IVP39_000011"
     CDS             complement(13728..14540)
                     /locus_tag="IVP39_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7585122.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14735..15292)
                     /gene="frr"
                     /locus_tag="IVP39_000012"
     CDS             complement(14735..15292)
                     /gene="frr"
                     /locus_tag="IVP39_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7585123.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15295..16017)
                     /locus_tag="IVP39_000013"
     CDS             complement(15295..16017)
                     /locus_tag="IVP39_000013"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7585124.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16153..17034)
                     /locus_tag="IVP39_000014"
     CDS             complement(16153..17034)
                     /locus_tag="IVP39_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7585125.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17133..17915)
                     /gene="rpsB"
                     /locus_tag="IVP39_000015"
     CDS             complement(17133..17915)
                     /gene="rpsB"
                     /locus_tag="IVP39_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7585126.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18274..18753
                     /locus_tag="IVP39_000016"
     CDS             18274..18753
                     /locus_tag="IVP39_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7585127.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18970..20061
                     /gene="phnW"
                     /locus_tag="IVP39_000017"
     CDS             18970..20061
                     /gene="phnW"
                     /locus_tag="IVP39_000017"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7585128.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20054..20707
                     /locus_tag="IVP39_000018"
     CDS             20054..20707
                     /locus_tag="IVP39_000018"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7585129.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQN"
     gene            complement(21184..22875)
                     /locus_tag="IVP39_000019"
     CDS             complement(21184..22875)
                     /locus_tag="IVP39_000019"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7585130.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23297..24244)
                     /gene="arcC"
                     /locus_tag="IVP39_000020"
     CDS             complement(23297..24244)
                     /gene="arcC"
                     /locus_tag="IVP39_000020"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7585131.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEAHVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24359..25378)
                     /gene="argF"
                     /locus_tag="IVP39_000021"
     CDS             complement(24359..25378)
                     /gene="argF"
                     /locus_tag="IVP39_000021"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7585132.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25469..26698)
                     /gene="arcA"
                     /locus_tag="IVP39_000022"
     CDS             complement(25469..26698)
                     /gene="arcA"
                     /locus_tag="IVP39_000022"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7585133.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            27159..27860
                     /locus_tag="IVP39_000023"
     CDS             27159..27860
                     /locus_tag="IVP39_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7585134.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(28432..29448)
                     /gene="tsaD"
                     /locus_tag="IVP39_000024"
     CDS             complement(28432..29448)
                     /gene="tsaD"
                     /locus_tag="IVP39_000024"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7585135.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(29445..29909)
                     /gene="rimI"
                     /locus_tag="IVP39_000025"
     CDS             complement(29445..29909)
                     /gene="rimI"
                     /locus_tag="IVP39_000025"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7585136.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(29916..30458)
                     /gene="rimI"
                     /locus_tag="IVP39_000026"
     CDS             complement(29916..30458)
                     /gene="rimI"
                     /locus_tag="IVP39_000026"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7585137.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(30442..31266)
                     /gene="tsaB"
                     /locus_tag="IVP39_000027"
     CDS             complement(30442..31266)
                     /gene="tsaB"
                     /locus_tag="IVP39_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7585138.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(31355..32335)
                     /locus_tag="IVP39_000028"
     CDS             complement(31355..32335)
                     /locus_tag="IVP39_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7585139.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(32359..33843)
                     /gene="galT"
                     /locus_tag="IVP39_000029"
     CDS             complement(32359..33843)
                     /gene="galT"
                     /locus_tag="IVP39_000029"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7585140.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(33855..34844)
                     /gene="galE"
                     /locus_tag="IVP39_000030"
     CDS             complement(33855..34844)
                     /gene="galE"
                     /locus_tag="IVP39_000030"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7585141.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            35093..35260
                     /locus_tag="IVP39_000031"
     CDS             35093..35260
                     /locus_tag="IVP39_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7585142.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(35322..37133)
                     /locus_tag="IVP39_000032"
     CDS             complement(35322..37133)
                     /locus_tag="IVP39_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7585143.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(37130..37495)
                     /locus_tag="IVP39_000033"
     CDS             complement(37130..37495)
                     /locus_tag="IVP39_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7585144.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(37658..38053)
                     /locus_tag="IVP39_000034"
     CDS             complement(37658..38053)
                     /locus_tag="IVP39_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7585145.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(38071..39033)
                     /locus_tag="IVP39_000035"
     CDS             complement(38071..39033)
                     /locus_tag="IVP39_000035"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7585146.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(39033..39245)
                     /locus_tag="IVP39_000036"
     CDS             complement(39033..39245)
                     /locus_tag="IVP39_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7585147.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(39266..39964)
                     /locus_tag="IVP39_000037"
     CDS             complement(39266..39964)
                     /locus_tag="IVP39_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7585148.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(39984..40526)
                     /locus_tag="IVP39_000038"
     CDS             complement(39984..40526)
                     /locus_tag="IVP39_000038"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7585149.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTFSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(40658..41662)
                     /locus_tag="IVP39_000039"
     CDS             complement(40658..41662)
                     /locus_tag="IVP39_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7585150.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFVLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(41659..42648)
                     /locus_tag="IVP39_000040"
     CDS             complement(41659..42648)
                     /locus_tag="IVP39_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7585151.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(42645..43451)
                     /locus_tag="IVP39_000041"
     CDS             complement(42645..43451)
                     /locus_tag="IVP39_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7585152.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            43617..44573
                     /locus_tag="IVP39_000042"
     CDS             43617..44573
                     /locus_tag="IVP39_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7585153.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACFPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(44650..45168)
                     /locus_tag="IVP39_000043"
     CDS             complement(44650..45168)
                     /locus_tag="IVP39_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7585154.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(45256..45405)
                     /gene="rpmG"
                     /locus_tag="IVP39_000044"
     CDS             complement(45256..45405)
                     /gene="rpmG"
                     /locus_tag="IVP39_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7585155.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            45635..46081
                     /locus_tag="IVP39_000045"
     CDS             45635..46081
                     /locus_tag="IVP39_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7585156.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDSRAIPIA
                     IDQAKEWYENASESERRTFEKEKNPSKSDKHDTNPRAGKLLDSDVIVEYAEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLVIYKKDGTKEKEIDY"
     gene            complement(46275..48170)
                     /gene="asnB"
                     /locus_tag="IVP39_000046"
     CDS             complement(46275..48170)
                     /gene="asnB"
                     /locus_tag="IVP39_000046"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7585157.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(48495..49469)
                     /locus_tag="IVP39_000047"
     CDS             complement(48495..49469)
                     /locus_tag="IVP39_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7585158.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVANYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNVDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLDVYSRGMGGLGLPGVL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(49808..49895)
                     /locus_tag="IVP39_000048"
     tRNA            complement(49808..49895)
                     /locus_tag="IVP39_000048"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49861..49863),aa:Ser,seq:cga)
     gene            complement(50040..50606)
                     /locus_tag="IVP39_000049"
     CDS             complement(50040..50606)
                     /locus_tag="IVP39_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7585159.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFCEIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            50862..52193
                     /locus_tag="IVP39_000050"
     CDS             50862..52193
                     /locus_tag="IVP39_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7585160.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGAFFLFIGLLSGTPT
                     DNWSGLLTILTSPSNLLTDYFDLGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVSNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            52159..52512
                     /locus_tag="IVP39_000051"
     CDS             52159..52512
                     /locus_tag="IVP39_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585161.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASPRSYY"
     gene            complement(52604..53023)
                     /locus_tag="IVP39_000052"
     CDS             complement(52604..53023)
                     /locus_tag="IVP39_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7585162.1"
                     /translation="MMTKYEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLRAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITSGNSEFLEPVTDEVYEGLENQ"
     gene            complement(53024..53368)
                     /locus_tag="IVP39_000053"
     CDS             complement(53024..53368)
                     /locus_tag="IVP39_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7585163.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISLLISGGNFEQLPAHLKIGKQNGITGEEIAEIITHLAFYVGWPKAWSAFNLAKEI
                     YLEGNSVNKKEA"
     gene            53718..54314
                     /locus_tag="IVP39_000054"
     CDS             53718..54314
                     /locus_tag="IVP39_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7585164.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            54438..56237
                     /locus_tag="IVP39_000055"
     CDS             54438..56237
                     /locus_tag="IVP39_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7585165.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMMILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSFFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSIVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(56515..56727)
                     /locus_tag="IVP39_000056"
     CDS             complement(56515..56727)
                     /locus_tag="IVP39_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585166.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            57605..57991
                     /locus_tag="IVP39_000057"
     CDS             57605..57991
                     /locus_tag="IVP39_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7585167.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            58035..58247
                     /locus_tag="IVP39_000058"
     CDS             58035..58247
                     /locus_tag="IVP39_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7585168.1"
                     /translation="MLILENKKIEWVFEVTFLFFQSLLEDIKDFLGHKDISTTQVYAH
                     ISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(58352..59698)
                     /locus_tag="IVP39_000059"
     CDS             complement(58352..59698)
                     /locus_tag="IVP39_000059"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7585169.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(59810..61159)
                     /gene="gdhA"
                     /locus_tag="IVP39_000060"
     CDS             complement(59810..61159)
                     /gene="gdhA"
                     /locus_tag="IVP39_000060"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7585170.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKGVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHACRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            61276..62529
                     /locus_tag="IVP39_000061"
     CDS             61276..62529
                     /locus_tag="IVP39_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7585171.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAV
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHLLTEKSKTTKKNLVNAWHGLLPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIHPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            complement(62600..63085)
                     /gene="ybaK"
                     /locus_tag="IVP39_000062"
     CDS             complement(62600..63085)
                     /gene="ybaK"
                     /locus_tag="IVP39_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7585172.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(63108..63866)
                     /gene="aroD"
                     /locus_tag="IVP39_000063"
     CDS             complement(63108..63866)
                     /gene="aroD"
                     /locus_tag="IVP39_000063"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7585173.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            complement(63882..65060)
                     /locus_tag="IVP39_000064"
     CDS             complement(63882..65060)
                     /locus_tag="IVP39_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7585174.1"
                     /translation="MKVQVKKRASSRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEELFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIIDYYAGYAVFSWYNETLYAHRGLLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            65290..67410
                     /locus_tag="IVP39_000065"
     CDS             65290..67410
                     /locus_tag="IVP39_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7585175.1"
                     /translation="MKYIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     VQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQIQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSQHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            67633..68358
                     /locus_tag="IVP39_000066"
     CDS             67633..68358
                     /locus_tag="IVP39_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585176.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            68348..68857
                     /locus_tag="IVP39_000067"
     CDS             68348..68857
                     /locus_tag="IVP39_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585177.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKEDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(68927..70375)
                     /locus_tag="IVP39_000068"
     CDS             complement(68927..70375)
                     /locus_tag="IVP39_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7585178.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFTVRLPDVEIKEE"
     gene            complement(70375..71091)
                     /locus_tag="IVP39_000069"
     CDS             complement(70375..71091)
                     /locus_tag="IVP39_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7585179.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(71072..71422)
                     /locus_tag="IVP39_000070"
     CDS             complement(71072..71422)
                     /locus_tag="IVP39_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7585180.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            71566..72339
                     /locus_tag="IVP39_000071"
     CDS             71566..72339
                     /locus_tag="IVP39_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7585181.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            72517..72883
                     /gene="ssrA"
                     /locus_tag="IVP39_000072"
     tmRNA           72517..72883
                     /gene="ssrA"
                     /locus_tag="IVP39_000072"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            73103..73405
                     /locus_tag="IVP39_000073"
     CDS             73103..73405
                     /locus_tag="IVP39_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585182.1"
                     /translation="MLMEGKTQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDNLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDVVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            73630..73941
                     /locus_tag="IVP39_000074"
     CDS             73630..73941
                     /locus_tag="IVP39_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein"
                     /protein_id="HAP7585183.1"
                     /translation="MVKENLELEDIHQKSKVIANEVMVTASKASVPLTSNEKADIEQV
                     FSEKTIELSERADRILKDQPSLSENELAIKLIKEDLKNASMFSPMKRILKKAIKHLEE
                     N"
     gene            complement(74130..74912)
                     /locus_tag="IVP39_000075"
     CDS             complement(74130..74912)
                     /locus_tag="IVP39_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7585184.1"
                     /translation="MSGDVWAFQNHWRGSLGWGTGGYHEIILRDGTVQLCYRDNDVTN
                     GVGNHNTVTYHICLVGNGSFTEAQERAFDVRARDAMRRFGLTSANVLGHNEFSGHGSN
                     QCPGVNMSNVRRRLADTAQTHDEIIAASAPKTVGNYVGKLEKFNELKLGVFRIGAWLV
                     PQNGAPSLGYGFVFWMDADNPNVEVGRCRSAGIVRDDVNAAYGLPSGLRFGLDGTLDI
                     RRFAGRKVFPMLRRTNDPNGDTINGQTVDIRFPEYVLTIPNR"
BASE COUNT        21392 a        15560 c        13135 g        24850 t
ORIGIN      
        1 atttcacagg atttgcggga gtttttgatt agtctttgtt caagtcgcga agcaattcat
       61 cgatatggtt gccatagaca actgattcat caagttcaaa gaacaattca ggtgtcttat
      121 aaatacttag attgtgccct aactctcgac gaatcaatcc tgatgcttta ttcaatccat
      181 cttgtgctgt tttcttatct gatgctaagt cagataaaat gctgtaatag atcgttgctt
      241 gctgtaagtc ccccgtaact ttcacatctg tgatcgttac gttctccaca cgagggtcac
      301 ggaccttttt gcgcaaaata tcattgactt cttttaagat ttcttgaccg actcggcggt
      361 cacggtagtt agccataatg attggcctcc ttttatcagt ttttagtcat ttttgacttc
      421 ttccatgata aatccttcaa ttacgtcatc tactttgatg tcattgaatt tttcaatcat
      481 tgcaccacat tcataaccca ttttgacttc tttcacgtca tctttgaatc gtttcagact
      541 tgcaagttgg ccttcataga ttacgattcc gtcacggatt acacggacac cgctatctct
      601 acgaatatat ccttctgtta cataggcacc tgcaatcgta ccaactttag aaactttgaa
      661 ggtttcacga acgatcattt gaccggtgat tttttcttca aattcagggt ctagcatccc
      721 tttcatcgca gtttcgattt cttcgatggc tttatagatg atacggtgaa gacgaatatc
      781 gacttcttca gtatcagctt gcacttttgc ctgcggtgta ggacgaacgt tgaaaccgat
      841 gataatagcg ttgctggctg ctgctaaggt tacatcactt tcattgattg ctccgacagc
      901 tgaatggacg atttttacac ggacaccttc cacatcaatc ttcttcaatg atgcagccag
      961 tgcttctgct gaaccttgta catctgcttt gatgattacg ttaacttctt tcaattcgcc
     1021 ttctttcagg ctttcaaata agttatccaa agtcacacgg cttgtggcag cacggttttc
     1081 taataatgca cgtttcgcgc gttcttcacc cgctgcacgt gctgtctttt cgtcttcgaa
     1141 gactacgaaa cggtcacctg cttgaggtac atcattcaag ccagtgattt ctactggcgt
     1201 tgcaggtcct gcttctttat cgcgacggcc aagatcgttt gtcattacac ggacacgtcc
     1261 gtaagtatta ccgacaacga ttgggtcacc aacatgaagt gttccttctt ggacaagtaa
     1321 ggttgacaca ggacctttac ctttatcaag acgtgcttcg atcactgttc cgattgcgcg
     1381 ttgtgttgga tctgctttta aatcttctac ttctgcgact aacaggatca tttccagcaa
     1441 ttcttcgatg ttttggccga atttcgctga gatctcaaca aagatcgttt ctccacccca
     1501 tgcttcagga attaactcat actcacttaa ttcttgcata acatgttgag ggtttgcacc
     1561 tggtttatca attttattta cagcgacgat gattggaacg cctgcagctt tggcatggtt
     1621 gattgcttca actgtttgtg gcatgacgcc atcatctgca gcaacaacta agatcgtgat
     1681 atctgtgata cttgctccac gtgcacgcat acttgtaaag gccgcatgtc ctggtgtatc
     1741 caagaaagtg attggtttac catcaatatc gatctggtag gcaccgatat gctgtgtgat
     1801 tccgccggct tcgccgcttg ttacacgtga atggcgtaat gtatccaata aagttgtttt
     1861 cccatggtct acgtgtccca taatcgttac gactggcgga cgtgagacta atttatctgg
     1921 atcgacttct tctgcctcga agaatttatc gatatcagca atgtcaactt ggactttttc
     1981 ttgtggttcc atgccatagt ccgtcgctaa cagttcaatc gtatctttat ccagtgcttg
     2041 gttttgattg accatgacac caagcataaa taatttttta atgatttctg ctggctcacg
     2101 atgaattttt ttcgcaatat ctgctacgtt cataccttca gtatactcta atacatctgg
     2161 caattcacgg aatttgcgtg gtggtacagc tggtttagtt gattgttgct gtttgccttt
     2221 tttacctttt ttattataac gattacggtt attattatta aatttgccgc gattgttatt
     2281 gtttggtttg ttgttattgt tagtacggtt ttgattgccg ccttggctcg ttccttgacg
     2341 attttggctg ctggtatttc ccgtattatt ttggtttgtt gttggttttt ttccttgatt
     2401 cacttgaccg ctcccttgac cgcgatcttg ataactgcgg ttatttttat ttttttgttg
     2461 attgttcgtt gtttcttttt tacgttgatc gttcggctgt tgattggctg gttttctgct
     2521 tgcttgttgt gcagcttgtt gtttcgtgct tgggtttttg aacgcttgtt gcaatttctt
     2581 ctcatcattg ctagtaatcg cacccatatg gtttttcaca tcgatgccaa gttgttgtgc
     2641 tttttccacg acgtctttac tagatttttt gagttctttt gctaattcat agattctttt
     2701 gttgcccatg caatcacctt cctaaccttc aattagctcc ttggtcttct tcgcaaatcc
     2761 ggcatccatt attccgaata ccttgcgagg ttttccgatc atctgcgtga tttctgcttc
     2821 agaaaatagc tcaaagcaag gaacttcata atatgaactc ttatctttta tcttttttct
     2881 tgtattctcg cttgcatcgg ttgccacaaa caccaatttt gctttttgtt tgcggatttc
     2941 agcaatggtc agttcttcac ccgtgattag ttttcccgcg cgcatcgcta gaccgatgag
     3001 attcatggct ttttgccgat taactgtatt cattttccaa ataactcttt acgtgctttt
     3061 tggtgagtga catagtcaag aagttcttga taaaactcat ctgttaatgt tgtttcgagt
     3121 actcgatcga gaattttttt atcccaagct tgttgtactt cttccggctc aagtgagaca
     3181 tacgctcctc gtccaggcat ttttccagtt ggatcgatcg atacttcccc ttcttttgaa
     3241 cgggtgattc ggaccatctc ttttttaggt ttcatttcac ctgaaaccac ggatttgcgt
     3301 aaaggaattt tcctttgttt catctctttg cctccttact aaagttaagc ttctggtttt
     3361 tcttcgaccg tttcattgtt gaatgcaatt gtttcatatt catcgtctgt taaatctgac
     3421 tgaacgattg cttcatcatg cagttcttct gtttcttttt gttgttcttg attttcataa
     3481 aattccgtca tgtcagattc tgatttgata tcgattttat ggttagtcaa tttagcagct
     3541 aaacgtgcgt tttgtccgcg tttaccgatt gctagcgata attggtagtc tggaacgaca
     3601 actgtgcagg cctttggatt tgtctcatcg aagatcacat ccatgacttg tgcaggattc
     3661 aatgcatttg caatgaatac agctggatct tcattccatt cgacgatatc catgttttcg
     3721 ccttttaatt cattgacgat tgcttgaact cgttgtccct ttggcccgac acatgtaccg
     3781 actgcatcga tatttggatc atttgagcga actgccactt ttgaacgatc gccagcttca
     3841 cgggcgatgc tgacaatttc aacgattcca tcatagactt ctggtacttc ttgctcaaat
     3901 aagcggcgca ataggtctgg atggctgcgg ctaacaaata cttgcggacc tttagacgta
     3961 ttttctactc gggaaacata aactttgatt cgatcatgtg gttgataaaa ttcatttggc
     4021 atttgatctt gtttagaaag aactgcctca attttaccaa gattgacata aatataacgt
     4081 ttatcctggc gttcgacgat tccttgcatg atgtcttttt cataagcact gaattcgtta
     4141 taaataatcg ttctttctgc ttcacgtaca cgctgtaaaa taacttgttt agccgtttgc
     4201 gcagcgatcc gcccaaaatc ttttggtgtt acttcaaaac ggatcgtatc accgatctca
     4261 tatgctggat tgattaaaag tgcatctttc aatgagactt ccaactgtga atccatcact
     4321 tcttccgtta cttcttttac cgcatagaca tggatctttc cttttttctg atcaaattcc
     4381 acttcgacgt tttgtgcttg gccatagtga cgtttgtatg cagaaaccaa tgctgcttct
     4441 aaagcatcga tcacgatttc ttttgagatc cctttttcag cttctaaagc gtctaacgca
     4501 tttaacattt ctttactcat tttttctgat tcctctccgt gattaaaatt ggatagctaa
     4561 gcgagctttt gcaatatttt ttcggtcaaa agtcaattct ttttctcgtg tttttatgcg
     4621 tattttcaat gtaagctgtt cagcatcaaa tgattgaaga aatccttcat attgtttttc
     4681 gccatctact ggttggtata aggagacatg gacataatca ccaagtgcct tttcatagtc
     4741 gctttctttt ttcaatggcc gctctgctcc cggggaggat acctccagga aataagcttg
     4801 aggaatcgga tctggatctg ctgcatcaag tttttcactc agtttctcac tgacaaaggc
     4861 gcattcttcg atatcgatac cgccttcttt gtcaataaat actcgtaaga accagctttt
     4921 tccttctttt acaaattcga cttcgacaag ttcaaaattt tgttcatcta aaatcggtgt
     4981 caccatttct gtgactgttt caacgacgct actcaaaagt ttcgcctcct taaaaaatta
     5041 agtgcgtaat tccattaaaa agagtgagcg aaaaatccgc tcactcacag ttagtatcat
     5101 tacctataag aactatacca tactctgctt aaaattacaa gcagacagga aaacagatgc
     5161 ttatagcata tcgaataacg ataattgatt ttcatctggt agatctttca atacgccatt
     5221 gtcgttcata tattcgatca atgtttttga aactttaccg cgggtggcaa gatcttcttt
     5281 ggataaaaat ggaccgtctt ttcttgcttc aacgatttgt tttgccacgt tggttcccaa
     5341 acttgggaca gctctaaatg gtgcgatcaa cgtatcacct tcaataacaa agttaacggc
     5401 atccgattta tacaaatcaa tcattccaaa tttgaatcct cgttccagca tttcattggc
     5461 taactccagc actgtcagtt gattcttttc ttttacggaa gcatctaatc ctttgtccgt
     5521 gatttctttc attgcttgtt tcacagcatc ttttcctttg cacatggaga ctagatcaaa
     5581 gtcgtctgca cgtacagaaa agtatgcaca gtaatacaaa atagggaaat aaaccttgaa
     5641 ataagcgaca cgaagagcca tcaatacgta agctgcagca tgggctttcg gaaacatata
     5701 tttgattttt gaacaagaat cgatatacca gtcgggcaca ttattttctt tcatcgccga
     5761 tagataggtt tctcgcaatt catcagggat cttgttccat tgtcctttac gaacagtttc
     5821 catgatttta aatgccatcc cgctatctaa tccagcatgg atcaaataaa ccataatatc
     5881 atcacgacag ccgattactt ccgccagtgt cgcatctcct cgtcgaatca attcttccgc
     5941 atttcctaac catacgtctg taccatgaga tagtccggaa atttgcagta gttctgcaaa
     6001 agttgtagga tgcgtttctt ctaacatccc tcgaacaaaa cgagtgccga attctggaat
     6061 acctaatgtc cctgttttgg aataaatttg ttcctgactt actccaagta cttcaggacc
     6121 ggcaaatatc cgcatcactt ccgggtcatc cgtagggatc gtttgtgggt cgatccctga
     6181 taaatcttga agcatccgaa taacagttgg gtcatcatgc cctagaatat ctaatttcaa
     6241 tacattatcg tgaatcgagt ggaaatcaaa atgagtcgtt ttccattctg aattttgatc
     6301 gtctgctgga tattggatcg gcgtaaaatc atagacatcc atataatcgg gaataacgat
     6361 gatcccccct ggatgctggc cggttgtccg tttgactcca gtagcgcctt ttgctaatct
     6421 gtcgacttct gcacttcgaa actgcagatt attgtccctt tcgtatcctt ttacatagcc
     6481 atatgctgtt ttgtcggcga ctgtaccaat cgttccggca cggtaaacat agtcttcgcc
     6541 aaacaatact ttcgtatagt tatgggcttc tgcttgataa tcacctgaga agttcaaatc
     6601 aatatcgggc actttgtctc catggaatcc taaaaatgtc tcaaatggaa tatcatgacc
     6661 gtctttgttt aagcgtgcac cacattttgg acattgtttt tctggcatgt caaatccaga
     6721 accatacgta ccatcttcaa aaaactcgga atattggcac tctggacaat aataatgagg
     6781 ggctaatgga ttaacttcag tgattcctgt catcgtcgca acaaagctag agccaacaga
     6841 gccacgtgaa cctactagat agccatcttc attactttta tgcactagtt tttgggcgat
     6901 cagataaata accgagaagc cattaccatt aatagaattc aactcttttt ctaatcgttt
     6961 ttgaatgatc tcaggtaacg gatctccgta catttgtttt gcttttgtgt aactcaattc
     7021 tgatatttca tcttctgaac ctggaatctt cggtgtataa agttcatctt tgactggaat
     7081 cacttcttcg cagatatctg cgattttatt cgtattttct actacgatct ctttcgctgt
     7141 ttcttcccct aaaaagtgaa atgcagttag catctcatcg gttgttcgga aatggacatc
     7201 cggcaaacta tgacgattca acggattcgc tccgcccata gaattgatca aaatttttcg
     7261 gtaaatggca tcttcttcgt tcagataatg tacattacct gttgcaacaa cgattttatc
     7321 caaacttttt ccgatttcta ccaaattttg gataatttct tccaaatcat gttcattttt
     7381 taccaattct tgctcgatca aaggcgcata gaccgctttt ggcataactt caatataatc
     7441 atagaatttt gctcggtttc ttgcctcttc tacccctttt tgcatcatgg cttcgaatat
     7501 ttctccctta tcacaagctg agccgatcaa tagattttca cgcatctttt tcaattggga
     7561 gcgtggtatt cgtgggacgc gttcgaagta ttcgacattc gacattgaaa tcagtttgaa
     7621 caaatctttc agtcctgcct gattttttgc taaaatcgtt acatggaaag gtcgagccct
     7681 tttgtaagaa tctccttctc cgatatgctc atttagctga tcatgataaa gcatgttatg
     7741 attgtccatt gcctctttca caaagatcca tgctaagtga ccagttgctt cagcatcata
     7801 gattgctcgg tgatgctgtt ccaagctaac accaaacttt ttagataaca cacctaatcc
     7861 gaaacgtttg aattgaggat acagatatct agctaattcc aacgtatcaa tgactggatt
     7921 ggccgcttca ggaataccat accttgcgta gctagtattc aagaacccca tatcaaaagc
     7981 cgcgttgtgg gctaccaaaa ttgtatcttt tgaaaattca aggaacatcc gcaatacttc
     8041 ttcttccgac tttgaccctc gaaccatttc atccgtgata ccagtcagat ctaccgttgt
     8101 tcgtgacaat ggatgtcctg gatcgataaa ttcatcaaag ctttcgatca catttccttt
     8161 atacattttg actgcggcta gctcgatgat cgtgtcatat actgcagaca atcctgtcgt
     8221 ttccacgtca aatacgacgt atgtggcttc actcaatgct tcatgtgcat cattataagc
     8281 aattgggaca ccatcatcta cgacattcgc ttccacgccg tataagattt tcacacctgc
     8341 tttttttcca gcactatgtg cttccggaaa ggcttgcgca ccaccatggt ctgtaatggc
     8401 gatagctcgg tgtccccatt ttcctgcttg tgccacaaga tcagagatgc tgtttgttgc
     8461 atccatcgta ctcatattgc tatgaacatg aagctctact cgtttttccc cttcaggagc
     8521 ataatctttg cgaggtgtat gtttgacttc tatgatatct tgcgcattca tgaccaaatc
     8581 acggacaaac gtatcttctt gaatacttcc tcgtaccttt aaccagctac cgacactgat
     8641 cgcatcaaat acttgttcat ctttttcgcc gttcgagaat tttttgacga taaatgatga
     8701 agtataatct gtgattttca aagtcaagat cttgcgtttt gaacgtagtt ctctgacttc
     8761 tttgtcgaat acgtacccct caagtgtgac tcttcgttct tcttcaacga tactgatcat
     8821 tggtgtcgtt ggttcatcgg ctggaatatt tcgtcctagc tggatgggtc cttcaagagc
     8881 tggcgattgc tcttttcttt cctttttctt ttgttcgtgg acaactagac tttcagctgc
     8941 ttgcttcata aaggcttctg cctgttcttg tttgcgttct tcaaaaagtt tcaatacacg
     9001 ttccgcttgc tgctcatcca cttctggttc gatacgaaat tttgggaatc cgtaactgac
     9061 gaacaactct tctactaatg gcaagtattg ttgcttcaaa taagaaatag ctcctgtact
     9121 gtcgattggc aagatgactt tacgatcttt tatcacaggt acctgcgttt tcaatacttg
     9181 ctgtgctagt ggtgtatcgc attgctgatt ttccaaagct tgggcccagt aatcttgcaa
     9241 gagctgttca tcgaatgtct ggtcatctgc taagatattc agtttgactt gtgcaatatc
     9301 tttaaaagcg atagtcagat gctgagtcaa tgaccgatat agcataatag gcaaaatttt
     9361 ggaaaaatgt agcgtaaatt cccataaacg cgatttacga tggaccacta cactatccat
     9421 ttcaccagca gtaagcagtg gatgattttt ttctgcttcc tctagttgga tttgttccaa
     9481 cagtttttca aataaatctc gtttttctga caaagatata cgccctttct gatgagaaac
     9541 aatgcaattg atcgcatctt atcctcttct ttttcagtac cttttagtat actcgttttt
     9601 tctgagtttt acattaaaaa attggaactg cgattcaaat cgcagttcca attttaaaag
     9661 cgaatcatca gattgtcaaa aacagctgat tcagctattt ttattttctg tgttttaacg
     9721 cttattattc gccttggttc aagaggattg gtaaagtatt cgccaattct tctttacgta
     9781 cttcaaccat ttcgccagtt ttcttgattt tcacttctac gattccatcc actgcttttt
     9841 taccgacagt gattctgatt ggacagccaa tcaaatcaga atccgcgaat ttcactcctg
     9901 cgcgttcatt tctgtcatca accagtactt catagccagc ttttgtcatc gcttcttcta
     9961 gttcattcgt caggcttgtt tggtattcgt cttttagatt catttggacg atatgcagat
    10021 caaatggcgc aattcctttt ggccaattga ttccttgttc atctgaatgt tgttctacga
    10081 ttgctgataa taaacgactt acaccgatac cataacagcc cataatcaca aatttttctc
    10141 gtccattttc gtctagtaca gttgctccca tagattcact gtaacgagtg ccgattttga
    10201 atatatgtcc gatttcgatt cctcgagtaa aggcaagaac accgttgttg tctggtgaag
    10261 gatctccttc ttgtacaaaa cgaagatctt cataagaaag cacagtgaaa tcgcgttctg
    10321 gattcacatt tttatagtgg aatcctgttt cgttcgctcc agtaatggca ttcgctaaat
    10381 cttggacata acggtctgca tatactttta cgtcatctga aacaccgact ggtccgacag
    10441 aaccaaactc agcacctaga tatttttgtg cttcttcttc tgttgcttct tctaaaaagt
    10501 cagcagataa aaagtttttc agttttacgt cattgacttc atggtctcca cgtaccaaaa
    10561 caaggacagg ttgttcatcc gcaatgaaca atacagactt gatgattttt tgaggttcaa
    10621 cagaaaagaa atctgctact tcgtcaattg attttacgtt aggtgtagct actttctcca
    10681 attcaagctg tgtttcatgc gatttttttg atacatagta gcttgtagcc atctccaagt
    10741 ttgcagcata atcactttca gtcgaataac agatcgtatc ctccccgatt tctgagatcg
    10801 ccatgaattc ctttgagtct ttcccgccca ttgcgccgcc gtctccgatg atcgagcgaa
    10861 attctaagcc acaacgcttg aatacttcgg tataggcttt ttcgtaatct ttatatgtct
    10921 cgtccaaact ttcttccgtt gcatggaaag aatatgcatc tttcatgata aactctcttc
    10981 cgcgaagcaa accgaagcga ggtcgttttt cgtcacgata tttaggttgg atttgataaa
    11041 gatttaaagg taatttctta tatgaattga tttcattacg catcaaatca gtaaaagttt
    11101 cttcgtgagt tggtccaagg atcatttttc gatcattgcg gtctttgaaa cgatacaagt
    11161 ttggtccata tgtttcgtaa cgcccggatt cttcccaaag ttctgctggc aagatggcag
    11221 gcatcagcat ttctaccgct ccgatttttt caaattcttc ccgcataatg cgtttcagtt
    11281 tttccaatac acgatttgct aatggcagat atgaataaat acccgctgat acttggcgga
    11341 tatatccagc tcttagcaag atttgatggc ttaatacttc tgcatcattt ggaacttcgc
    11401 gtaatgttgg aatcaacatt tttgattgtc tcattctata tactccttta tttcctcaaa
    11461 atagttatcg tttgttatta taccctattt gtcaactttt tctagaaaaa gaatcgttga
    11521 atatcgttcc atgtaactag caccatcaac aacattaaaa atccaaagcc agctaatgtc
    11581 aatattcctt ctttttcttg acttaatggt ttcccgcgaa tcccttcaaa aatattcaag
    11641 actaatttcc cgccatctaa ggcaggaatc ggaagcagat tgacgattcc gagattcatt
    11701 gaaagaagcg ccatcaaact aataacagtt gttacacctt gatttgctgc ttctgaagac
    11761 aattggtaca tcatgacagg tccgcctaat ttatctaaac tgaaacctgt aaatagtgat
    11821 cctaaggctt tgaaaatttc caatgaaccg ctgaaagctt gacgcgtccc tccaatgatc
    11881 ttatccataa aacctgtatt cattggggct tttataccaa gttggccaac tttttcgcca
    11941 tttgactcta cagatttcgg tgtcacgtcg acagattgca cctgaccttc tcgttcgatc
    12001 ttaaagtcta atgttttacc tggatttttc gtaatgactg ttgtcagatc attccatgaa
    12061 tggatttctt ttccgtccac actgactacc tcatcatttt cttttaagcc tgcttctgcc
    12121 gcagctccat taggcatgat ctctcctacg cgattagtgt tcgtcacttg aacaccacct
    12181 tgcataaatg ccagaatgat gaataacacg attgccaaaa taaagttatt catgggtcca
    12241 gcaaagttgg tcagcatacg ctgccacaat ttagccgatt ggaactgtac gtcttttggc
    12301 gcaattctga tttctgttcc gtcgttttca ataatcgtcg catcgtgatc aacagggtag
    12361 gttactactt ctgtttcgtc tccattcaca taaccagtga tagtcagctc atcttctaaa
    12421 tcataacggc tcaattccat cgggatagca tttgtcaatt ggattttctt acttaaattt
    12481 atcttctcta cgatgccgtc agaatttagt aaaagagaaa gaggcattcc tggtgccatt
    12541 tctgtttcat catccccatt gcctgccatt cgaacataac cgccaatagg taataggcga
    12601 agcgtatatg ttgtcccgtc ttttgcttga tgcccataaa ttttcggacc cataccgata
    12661 gcaaattcgc ggacgaggat tcctgatcgt ttcgcaaaga agaaatgacc aaactcatga
    12721 acaatcacta atataccaaa aacgatgata aatgtcagaa tcgttttcat agcttttttc
    12781 tcctttactt gttttttgtg ttttttataa aacaacgata aaacactata attttaccat
    12841 agtataccaa agacaccaat agtatcaata taatgaaaaa taaaaacctg cgtcttttat
    12901 gaaattcaca aagaaacgca ggtatactta ctaaataatg ccaaataaat gcatgatagg
    12961 aaatacaaac agcaaactgt cgaaacggtc taagattcca ccatggcctg gtaagatatt
    13021 tcccgagtct ttcacatcat aatgacgttt gattgcagat tcgaccaaat caccaaattg
    13081 tccgactgct gaaaaaataa tcgtcatcag aagcatgaca aacataccat gattgaatag
    13141 ttcttttgct gggtatagca ataaataaag aaatgcgact acaacagcac taccgatacc
    13201 accgagcgca ccttcaatcg ttttgttggg cgaaatatca ggacttaact tatgctgacc
    13261 ataacgtcga ccaaccatat atgctccgat atcagttgcc caaacaataa atagaccaaa
    13321 tatcaaaaca gccaagcttt ctgttcgtgc attgacaaaa ttctggaaac ctattcccac
    13381 atacaagctg acaagcacag gaaaacctgc ttcatcaatc gtatacgtat ttttagaaat
    13441 aactgatgtt cctaaaagaa tcataactat aagataaaat aacataaatg tacttacatt
    13501 ctctggaaga aagaaaaacc atctttcttt tggcaaaaca agtaacactg caccaacggc
    13561 ggataaaact ccttcaaaac ttaagagtgt caatcctttc atccggaaca gctcgtaaac
    13621 tcctactacc gctaagattg ctgctgtaag ctcaaccgct atgcctccga tccatataat
    13681 cggaataaac aagactagcg ccacagctgc tgtgatcaca cgttgtttca tgactgatcc
    13741 cctttctcat cttctgtttt ttttacaccg ccgaatcgac ggtctcgatt ttgataagag
    13801 gcgattgctt cttcaagatg cgcaccatca aaatcaggcc aaagcgcttt tgtaaaatac
    13861 agctcactat atgcgatctg ccataacaga aaattactaa ttcgctcttc tccacttgta
    13921 cggatcatca gctcaggatc ttgtaactct ttaggcaaga aacctgtcat taaatgctta
    13981 gcaatcaatt catcatcgat ttcttctgcg tgtatttctc cttttgctac ttcttcggca
    14041 atttcttgga cagcagtcac gatctctgct ctacttccat aattcaaagc aaaattcaat
    14101 accattccag tattgttttt tgtttgctca attgcacgtc ttacagcatc ttgcgtatgt
    14161 tctggcaata cgttttcata acccatgaca tgaactttga cattttcttt aatcaattct
    14221 ggtacgaacg tatcaaagaa gtcgacaggt agttgcatga gaaaacttac ttcatctttt
    14281 ggtcgcttcc agttttcagt cgaaaaggca taaagcgtca acactttcac tccaagcctt
    14341 gatgcttttt ttgtgacttt ctttactgtt tccatgcctt ctttatggcc agcaactcgt
    14401 ggcaaacgac ggttttgcgc ccaacgacca ttgccgtcca tgatgatggc aatgtgctga
    14461 ggaattggtc catcggtatt aaattgaaat tcgctttttt cttgaatata tttgtttttt
    14521 tgcggaaaaa aacgtaacat tctttttcct cctatactta aaagattccc actatcttaa
    14581 gtattatagc aatatctaac tgtttttcct atgaaatcat tgttttttta tttaaaaact
    14641 gaataatttt ttcttttttt aatttttaaa taaaagaaat ataaataaaa acagaggaag
    14701 cggaatgatc ctcccctctg tttttatgat tttattaaac ttccaataat tctttttctt
    14761 tatcagcagc gatcttatct acttctttaa tgctgttatc tgtcaatgtt tgtacgtctt
    14821 tttctaatcc gcgcaaatca tcttcggtaa tatcaccatt tttttgttgt tttttatatt
    14881 catcgatcgc atcacgacgg atgttacgaa tcgcgatttt tgcattttct gcttcttttt
    14941 tcacatcttt agctaattct tttctgcgtt cttctgtcag ttgcgggata actaagcgaa
    15001 tgacatttcc atcattggtt ggactgattc cgatatcact tgccataata gctttttcaa
    15061 catcttgaag aatgctcttg tcaaaaggtg tgatcattaa aacacgtgct tctggaattt
    15121 ggattgatgc catttggttc aatggtgttg gcgcaccgta gtagttcaca gtaatacggt
    15181 ccaaaaggct agcgttcgca cggcctgcgc ggatttgccc aagttcacgt tgaagatttt
    15241 gtgctgcttt tttcattttt tcttttgctt ctgtcataat tgcatctgcc atttttattt
    15301 cccccttaca gttgttccga tattttcgcc taagattgca cggcgaatat ttccgtgttc
    15361 atttaaattg aagacaagca gtggaatatc attatccatg cttagtgaac ttgcagttga
    15421 atccatgact tgtaatcctt tggcaataac ttccagatga gtcagttctt caaattttac
    15481 tgcatttgca tctactttcg gatcagcaga ataaacaccg tctacattgt ttttagccat
    15541 caggataaca tctgcgccga tttctgccgc tcgtaatgct gctgttgtgt ctgtcgagaa
    15601 gtacgggttg ccggtaccgc ctgcaaagat aacgatccgt cctttttcta aatgacgttc
    15661 tgctttacgg cgaatatatg gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg
    15721 tgtcggtaca cccacgtttt ccaacgtatc ctgtaaagct aaggcattca taactgtagc
    15781 taacataccc atgtaatcag cttgagcacg ttccatgccc atttgtgctc cgatttgtcc
    15841 gcgccagata tttccgccac ccacaacgat tgccatttca acgcccagct cgtgaacttc
    15901 tttgatttct tggatgattt ctttgattac tggtggtttg attccaaatc ctgcatcccc
    15961 agctaaagct tctccactca attttaagac tacacgctga tatttcggtg tgaccatgtt
    16021 cattcctcca atattttcta ttgccatttt accatactta gatgatttcg acactctaag
    16081 actggttttt cttgttttgc ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt
    16141 tattcaacag aattattttt tcacttggct cataacttct tctacaaagt tatcttcgcg
    16201 tttttcgata ccttcgccaa cttcgaaacg tacgaatgtt ttaacagttg cgccttttga
    16261 tgcaacgtat ttttcaacag tcatatcagg atctttaacg aatggttggt ccaccaaagc
    16321 gatttctgct ttgaatttct tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg
    16381 tttgccttcg ttcaatgctt gttctgtaag aacagttttt tcatgttcta attcagcttc
    16441 tggaatttgt gattcgttta cataacgagg attgatagct gctacgtgca tagctacgtc
    16501 tcttgcaact gtttcgtcag ttgttccatc tagaacagtc aatacggcaa tacgtccgcc
    16561 catatgcaag tatccaccaa atgcagcatt gtcgtctttt tctacaactt caaagcgacg
    16621 gaagctgatt ttttctccga taacttgagt tgcttcgatc aagtcagatt caattgtacc
    16681 tttatctgtt ttgattttca ttgcagcttc catgtctgct ggtttgtttt cagcaacaag
    16741 ttctgcaatt tcttttacta aatcttggaa catttcgttt ttagaaacaa agtctgtttc
    16801 tgagtttact tcaacgattg ctgcagtgtt acctttcaca gcaactgaag ccaatccttc
    16861 agctgcaatg cggtcatttt tcttcgcagc ctttgccatt cctttttcac gtaaaagatc
    16921 aactgctttt tccatgtcgc cttctacttc aactaatgct tttttcgcat ccatcattcc
    16981 tacgccagtc atttcgcgta gttcttttac cattttagct gtaacgtctg ccatgtttaa
    17041 ctcctcctgt gtgtttttct ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg
    17101 cccagccttt gaaacagctc catcattcaa aattattctg ctgaagaatt gtcgccttca
    17161 acaacatcta cgatttcttc gattgatgtt gctgtgtttt cttctgtgaa atcttcttca
    17221 actacttgat cttcaccttg gtttccttcg atgaaagcat cagccatttt tgaagtaatc
    17281 aatttaacgg cacgaattgc atcgtcgttt gaagggatta ctacgtcgat ttcatctgga
    17341 tcgcagtttg tatcaaccat agctacgatt gggatattta atttgtgtgc ttcttgaaca
    17401 gcaatacgtt ctttacgtgg gtcaacgatg tacattacat ctgggattct tggcatatca
    17461 gcgataccgc ctaagaattt ttcaagacgt tcacgttctt tgttcaaacc agcaacttct
    17521 tttttaggta gaacttcaaa agttccatct tcttccattg cattgatttg tttcaaacgg
    17581 ctgatacgtt tttggatagt atcccagttt gtcaatgttc cgcctaacca acgatggtta
    17641 acgaagtatt gtcctgcacg agttgcttct tctttgattg cttcttgtgc ttgttttttt
    17701 gtacctacga ataatgctac gccgccttct tcagcaacgc tcttcatgta atcataagct
    17761 gcatctacta atttaactgt tttttgcaag tcaatgatgt agattccgtt tctttctgtg
    17821 aagatatatt tcttcatttt tggattccag cgacgagttt ggtgaccaaa gtgtacgccg
    17881 gcttctagta attgtttcat agaaattact gccatgtttg tttcctccga tttggtttta
    17941 tttttcctct tcttatcttc taccttgccg gcaaactttt tcaagcacct accgacaatc
    18001 caataagaat gtggatttgc tgatgcataa agcaccctca gctattatac agacaaaaga
    18061 tccgtatcgc aagagcaaat atacttttct gctcaacatt caatagttta cctgaaagat
    18121 aaaaagaatt caagaaaaac agtagatttc tctttgtttc tttattatcc tttttccata
    18181 aaataatttt aggtataaaa gacgaatatt attgggcaat ccttcttttt tttgttatga
    18241 tacatgtgaa atctttcgta aaggagaaag aaaatgcgca aagctcaaag acatttgctg
    18301 attaaacaag tcattgaaga attcccgatc cgtacgcaag aagaactatt aaacaaactt
    18361 gggcattatg gcgttaccgc cacgcaggca acgatttctc gagatatccg tgatctaaaa
    18421 atcgtgaagg cacctgatga aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga
    18481 actcctgaga ctaggactga ggaagaggat cggctggttc aaatgattga agacattgta
    18541 ttgaaagtcg aacgagtcca ctttttgacg atcgtcaata ccttgccaga caatgctcat
    18601 ttatttgcat ctgttttaga tgagatcaaa cctccctcta ttgtcagtac aattgctgga
    18661 ttcgatacga tcatggtcat ctcggaaaat gaggaggacg caaaacgagt agaagaattt
    18721 cttcaccatc caactgaagt atcacttttt taatcagagc acgtattcct aatatggaat
    18781 gcgtgttttt ttattttcaa tattttcctt attaaataaa cctttacact ctttacaaaa
    18841 atacatcatt ttttattcaa ttttttcaac accttgattg aatcacaata atcatcattt
    18901 atattggatg ttaacaagag cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa
    18961 ggaaatcata tgtataaatt attaactcct ggaccattga cgacttctga aacagtgaaa
    19021 aatgctatgc tgatcgatca ttgtacttgg gatcaagaat acaaagaaat tacacaatgg
    19081 ataagaaaaa aattattgga tcttgctcaa gtatcggaag gaatatacac aacggtctta
    19141 atgcaaggta gcggctcctt tggggtagaa tcggtattaa gttctgtgat caggccagaa
    19201 gatactgtac tgatttgttc aaacggcgca tatggacaac gaatgatcaa catgtgcgaa
    19261 tgtctgaaac tgaactatac cgtttatgag gtatcggaac atcaaatcct ccaagctcat
    19321 gtgattacac agctgattga agagaatccc tctatctccg cactagcaat ggtccatagc
    19381 gagactactt ccggtatttt gaataatttg gaagagattg ctgctgtcac acaagaaaaa
    19441 gatcttttgt ttatagtgga cgcgatgtca agctttggtg ggataccgat tcgtgtagcc
    19501 gaactaggga ttgatttttt gatttccagc tcaaacaaat gtattcaagg tgttccaggc
    19561 ttttcttttg tacttgctaa taaagcggaa ctagaaaagc gaaaaaatgt gagtcgaagt
    19621 ttgtcacttg atctatatga ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt
    19681 ttcacttccc ctacccatac ggttttggct tttcaacaag cgctgaagga actagaggca
    19741 gaaggaggaa tcgaaactcg atattcacgt tatctcgcca ataatcatgt actacgacaa
    19801 aaaatgacag agatggggtt ccagcctttc attcaagaat atcaggggcc attcatcacc
    19861 tcttttcttt accctaagga ctggcgattt gattttaatc atttttatca gtatttaaag
    19921 caaaacggat ttgtgatcta tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga
    19981 aatattggcg agatctatcc ggaagatatt ttggaactga cagcaattat caaagactat
    20041 aaggagaacg ttaatgatta aaactgtaat ttttgattgg gcaggaacaa ctgtggactt
    20101 tggctgtatg gctcctgtcc acgcttttag aaatgctttt ctagaaaaag gaatccaact
    20161 gactgacaaa gaaatccgcg aaccgatggg aaaattaaaa tgggatcata tccaacagct
    20221 tctttccctc ccttctgtca aaaaacaatg gatagctatt tatggacaac tacctcagga
    20281 aaaagaggtg gaggaattgt atcaacaatt tgaacattat ctgtttaaag atttggtcaa
    20341 gcattctacc ttgaaacccg acacattgga aacgatcgaa cggttgagaa acgctgggat
    20401 caatattggt tccacgactg gatttactgc tcctatgatg acaatcgtag cagaaaccgc
    20461 aaaaaaagaa gggtattcac cagacttcat ggcaacttct gaagacgtcg aagggtatgg
    20521 tcgaccgttt ccttatatga tccataaaaa catgcagtac ttcaaaaaca agtcggttga
    20581 tgaagtaatc aaagtcggag atacggtctc agatatccag gaagggaaaa atgccggagt
    20641 gttgacagta ggtgtaattg aaggtagttc gctaatgggg atttcttatg atgagtacca
    20701 aaattagaga tggggaaaaa agatcgttta aaacagtctg ttgctcttag attccaacaa
    20761 gctggcgctg atatttgtgt aaacaattta aaagagttaa cagaatacat tttgtacgct
    20821 gacaagaatc cagaatgact tttatacaaa atcagaggtc gtgaaaaagc tgtactgcat
    20881 caagtaataa gaacagccaa acaaaaatag ctcctcatat ttttcgtttg gttaggctta
    20941 ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa aaaaaaggga gctgtgacac
    21001 gacttttgtc acagtcccct tatttacaaa caaacagtgt ttaagagaca acctctccac
    21061 taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa
    21121 gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt
    21181 ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac
    21241 agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt
    21301 cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac
    21361 tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt
    21421 aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa
    21481 tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt
    21541 ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt
    21601 tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt
    21661 ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa
    21721 tacttcttct aataagacga tctttccttt acgagttgat aattttttac caccttgcgt
    21781 gatcaaacca aatggaatat ggcgcatatc gtcagaccag tcaaagccca tttccttcaa
    21841 gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg
    21901 tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag
    21961 tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac
    22021 gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc
    22081 atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag
    22141 atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat
    22201 agcctcttca tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc
    22261 tggttctgtt tcagctactt catggaattg gacatataaa cgaagcaatt cattgattgg
    22321 ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa
    22381 ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat
    22441 aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt
    22501 ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga tattgctatc
    22561 cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt
    22621 attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc
    22681 gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa
    22741 tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg
    22801 ttcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag
    22861 atctttatta ttcatcttta ttcctcctaa agtgctccct tgattgttaa acaaaaaaaa
    22921 tctctagcga cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac
    22981 tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa
    23041 aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga
    23101 attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa
    23161 ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttctgat aaaaaaacaa
    23221 tccagatggc ctaaaggaga agaaatacca cctggatagc tataaatcat ttcgtaaata
    23281 cgaaagactt aacaaattaa tcagctacga taatcgttcc ttcttcagaa gctaaaagat
    23341 tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt tggttttgct tcaacaaatg
    23401 caatagctgc ctctactttc ggaagcatgc ttcctggagc aaattgcttt tcatcaatgt
    23461 attgtttcat ttctgaaaca gtaacagttt ctaatttctt ttgatctggt ttttggtaat
    23521 tgacatacac attgtctact cctgtcaata cgatcagcaa atccgcatcg atgatttcgg
    23581 ctaatttttc tgaagcaaaa tctttatcga ttacggcttc tcgtccttct aatccagtag
    23641 ctgtttctac aacaggaatc ccaccgccac caacagagac tgtgataact ccttgatcga
    23701 ccagcgtttc gatgacatgt gcttctttaa tcgagatggg tttaggggaa gcgactactt
    23761 ttctccatcc acgtccagca tcttctttga atgtcacagt ggagtcagca ttcatttgtt
    23821 ctttggcttc ttcttccgtg tagaatggac cgactggttt acttggattt ttgaatgaag
    23881 gatcattttc atccacaata acttgagtga ctaaagaaac cacatcttta tcgatccctt
    23941 tttccttcag tacttcgccc atggcatttt gtaaccagta accgatagat ccttctgtca
    24001 ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc agaatcagca gcttgctgct
    24061 gaatcaatag gttaccgact tgcggaccat ttccgtggga gatgatcaat tcatctcctt
    24121 gttcgataaa tttcactaaa tactttgctg tttccattaa cgcttcttgt tgggcttttg
    24181 cacttgcatc cgtagacaag attgcattac cccctaaagc gacaactact tttcgtttgg
    24241 ccatttcatt cgtcctttct tcagttctac cgaattttct agataacaaa acgagccagc
    24301 gttttgtgat tttccctcaa tgctgactcg ttttttcgtg agatgaatgt ggttatgctt
    24361 aaacgcgagg gataaacaag ttacctaatg ttgctgccat gatcgctttg attgagtgca
    24421 tacgattttc tgcttggtca aattgacgag catatttgct gcggaagact tcatctgtta
    24481 cttccatttc agtgatgcca aagcgttctt tcatttgttc accgtaaaca gtgtttgtat
    24541 catggaaagc tggcaagcag tgcaagaaga tcaaacgatc tgtgttgtgt gttttttcta
    24601 ccatttccat attgatttgg taaggtttca acaatttgat acgttcttcg aatttatctt
    24661 cttcacccat tgatacccat acatcagaat aaaggacgtc tgctccgtct acacctttat
    24721 caacatcgtc ggtaatcatc aattgtgcac cggatttttc agcaaagcct tctgccattt
    24781 tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac gatacgcata ttcgctccta
    24841 aaattgcacc tgtgactaat aaagagttag ccatattgtt tcgaccatct ccgcagtaag
    24901 caacagtgat accttctact gttccaaagt tttcttggat agttaaaaag tcagcgatca
    24961 tttgtgttgg atgccattca tcagtcaagc cattccaaac tggaacacca gagaattctg
    25021 ctagttcctc taccattttt tgactgaatc cacggaattc gattccatca aacatacgac
    25081 ctagtacttt agctgtatct tctgtggatt cttttttacc taattggata tcatttgcac
    25141 ctagatattc aggatgtgca cctagatcga tcgctgcagt agtaaatgca gaacgagtac
    25201 gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc ttctagatag tgatgtggga
    25261 taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc gatcaaatat tgtaattctt
    25321 cttttgtaaa gtctttttct gctaataagc ttcttccttg gaatactgat tctttcatat
    25381 taaacattct cctttagatt gtttttattt aagattaaag aggaaatccc gacggcagct
    25441 gacagtaaga ccgccgtcag gaaacaaatt acttcaagtc ttcacggaca agtggttggc
    25501 tcatgcagcg tggaccacca cggcctcgtg acaattcact tgaattgatt tctaacactt
    25561 tgatgccata gctacgaagc aattcgttcg atacataatt tcggttataa gtgactacaa
    25621 cacctggagc gattgccaat gtgtttgatc catcattcca ttgttctcga ggtgcaacga
    25681 tctcatctcc attaccagtt gggatcagta caagatcatc taatccaaga gcagctttca
    25741 tcgtttcgtg taagttatct gaatgagtaa tcttgatatc atcaccatct ggttcgattg
    25801 taaagacatc catcttaccg tctttacttt gaattgctgg atggatagtg aatttatcgt
    25861 gatctaccat tgtaaatacg gtatccaaat gcatcattgc acggttgttt ggaattttga
    25921 ttgctaacac tttttcaaat cctgaatttt ttgcgaatag gtggcgtgct aatttttcta
    25981 aagctttcgc attagttcgt tgagaaatcc caacagctac tactttatcg cttaagatca
    26041 attcgtctcc gccttcgata tgatctggat tttcacgatc caaccatact tcgattcctt
    26101 tatttgcaaa tcttggatga tgtttcaaaa tatattctgt aaacatcgat tcacgacgac
    26161 gtgcttcaaa agtcatctta ttgactgtca tcccatttcc cattgatgcg gaagggtcgc
    26221 gtgtaaagta caagtttggc attgggtcca tgtaaaatgg atagtcatcg tctgctgaca
    26281 gatcgtatag actgctagaa cgtacttcaa tatcttttgt acgaacccca gccatgattt
    26341 tatctaccat ttcttgcgtt tccattgata gaaggaactc atgaagaccc tctcttacag
    26401 cattcgatgc aatatgagat tcatccaaca ttttattcaa gaattgttct ttgacattgc
    26461 ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag agtctcgacg ccttcatttt
    26521 gcaatgtctt tgcaaagttg tcatgttctt cttgagcaat cggaagatat ggaatatcat
    26581 cgaacaacag acgatccata atatctggtg tcaaattttc cacttcttgt ccaggccttt
    26641 tcagtaaaac tgtctttaat ttccctattt cagagaaaac gtgaataggt ttatccatgt
    26701 tgtgttgcct ccttcttttt gattacatgt ataggatatc ctccttttgt aatcactttc
    26761 ataattttgt ctcattcaaa atgagacatt cctcacatta atattcgata attaaataat
    26821 taaacgtttt tctattttta agcgtaattt attcattttt tattctgtat acactttcaa
    26881 aaaaatattt attcattgaa aaaaattttt tgaataaatt aaaaaaataa attatattag
    26941 cgttgttagc gtttactata tgtattattg ttgtttttta tacacaaatc attcgcttca
    27001 aaacaaatta ttcaatacca ttgtatattt tttacataat aacaaattat atttgtaaag
    27061 tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt aaaaaaagtg
    27121 acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat aaaatcgatt
    27181 ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat gaagagttcc
    27241 aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc cttttcgacg
    27301 atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt ttagaacgtt
    27361 atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac ctttttccta
    27421 tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg actgatattg
    27481 aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat cccgaacagt
    27541 tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg cgtatccaaa
    27601 tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt ttgaaagaag
    27661 aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc accttaaaag
    27721 aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc aaacaactac
    27781 gtgattccgg actgttagat tatgacaaaa agcgttttac tttctgtaag acagctacta
    27841 ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata tttatcaggg
    27901 tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag atcactgggc
    27961 aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt tgcaaaaaat
    28021 actgatagat taactagagg aagcgtcttg tcctcatccg acctagatta ttcgatattc
    28081 ctcgacaata aacgattaat cagtgagggc aatcagagga acggataatg atgaataagc
    28141 tgtaaaaaat agggctttga caaaagcgtg tcacagccct tatttcgatt aaacggtgtg
    28201 tacctgtcaa ctcttcggtc acaagtcata atgttcataa acatgaggag ctgttttttg
    28261 aaattgttcc ttgtgaatca gagttaaacg acaggcttac gaactcttga ttaaacggcg
    28321 ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc aaaaacatga gaagccgttt
    28381 ttcctgggct gttcttatta cttgatgcag ggcaaatttt tcacaacctc tttattcaga
    28441 taaaaccaag cttggatcag cattcaactg atagctagca aaatttcttt tttgcatttc
    28501 cacatgtgct gctgctccaa tcattgcagc attatctcca caaagtcgaa gagggggaat
    28561 cactagttct acctcaggga gtttagttgc caaagcatcc tgcaatcctt cacgcaaccc
    28621 ttgattggcc gctacacctc cagctacaat cagttgcttg actggataat tttgacaagc
    28681 tcgaagtgtt ttagtaatca atacatcgat cacacttgct tgaaagctag ctgctaaatc
    28741 atttttgtct agatcttctc cacgctgttg tgcattatgc accaaattga taaatgcact
    28801 ttttaatccg ctaaaactga aatcataatt atcttcatgg atcatcgctc gtggaaaatg
    28861 gtaattatct tttccctgat gagctagctg atcgatttct tttccgcttg ggtaagaaag
    28921 acctaatact cttccaactt tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc
    28981 aatgatttca tatgatccat cttcttgcat atagactaat tctgtatgtc ctccgctaac
    29041 aagaagtgcc atcaatggaa actggaatgg tttaaccaga cgggctgcat agatatgccc
    29101 tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc
    29161 agaaatacca atcagcaacg agccgacaag tcctggtcca taagtgacag cgactgccga
    29221 taaatcttct gccgacacac cagcttctac cagtgcatct tccaagcata aggtgatttg
    29281 ttctacgtga tggcggctag ctacttcagg aactacgcca ccaaaacgtt tatgactgtt
    29341 tatttgagaa gcaacgatgt tagataagat ctctgttcca tttctaacga ccgctacact
    29401 cgtttcatca caacttgatt caatcgccag aatcaattct tcattcattt catcgtctcc
    29461 gtttttaact ttctgctcat aatgatcgca tcttccgtcg gattgcgata atatgatttc
    29521 cgtttaccta atatacgaaa cttttctgat tcataaagtt ttcttgctgc ttcattagat
    29581 agacgtactt ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat cagtaattgt
    29641 aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg caacagcaat atttgtgatc
    29701 tcgacttcat ctattacttt cgagtatcca ataaagccac agatctggtt atcttcaact
    29761 aaaagcagat aatcagagtg aggctgttgg atgtctgcca aaaactgcat tttcgtccaa
    29821 ggtgaaccat gtggataagc attttcgcta atcatccata cgcattctgc caagtacgct
    29881 tcatcaaaat ctttttttct gcaaatcaac taatctcact cattttctaa aagttttatc
    29941 atatccaacg catcttcttt tgtttcagtg taatatcttt ttttgatcgt tgttgcttga
    30001 aaacctaact tccgataaag tctttgtgca tcttgattac tcatacgcac ttctaatgac
    30061 atcgtctcac agcgattcat tatagcaaag ttctcgattt cgtcaataag cagcgaaccg
    30121 attcgtttac gctgatatgc cggtcgaacg gcaatattcg tgatatggca atctaaaccg
    30181 aacgctcttg aaccgataaa accaatcatt cttccattat caaagatacc tagatataga
    30241 tgaggaagcg gtgacttcaa ttctgatata aaggcacttt ttgtccatgg caattcgcca
    30301 ttatagactt ctcgttcgac atccagcagc tctttgatat cttctgtagt caattctctt
    30361 agtacgtaac gacgatcgat ttgttcgatt gttttttctg tataaatcat tttatcttga
    30421 aaaaagcttc tggcatacgc tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg
    30481 cgtctccaac catttttctt ctgcttctac ccgttttaaa tagcgaggaa gaaaagcttg
    30541 tttctctgca actggttcag ctaaagcacc taattgagcc acagttacac cacttggaat
    30601 atcccatgct ggaactgtat taatcatcgc actaggcaat atttccttta tttcatagaa
    30661 gaatttgtga caatcactcc cgacgaaata aatagcttca ttcagctttt gtagttgtgt
    30721 gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca atttctaatc tattttcttt
    30781 ccagcggtat gccccagcgt atacattttt tcttcgagca tcaaacatgg gcacaataat
    30841 tttatccaca ccgatgcaat tagccgcgat ggttttcaga ctagatactc ctactagctc
    30901 tttatctaat gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa gccctgtata
    30961 agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct tttggactta tttgaagatc
    31021 tgcaaacaat tggtcgattg caggcatcag tgttacactg tggttgcgtt tgatatttgt
    31081 ttgtatttgt ccaagaattt gttcgtcttc taccacaccg atcgccaaag tttggtttgc
    31141 agtatctatt cctaatgtaa tcatatattt gatcctttct gctatgacat tctcgccttc
    31201 cattgtagca tactttttta ttctgaggat gaaaatacca aaggcaaaac tagtacaatg
    31261 atacatgatt atctgctggc gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg
    31321 gtatcacttc atttccccac agcttttcca tcttttattt cgttgattct cgaataatca
    31381 actctgtttc cacaataatc tttcttgaaa ccggtgcttt ggaattactt aattgaagaa
    31441 tcgtctccac tgctagctct cccatccatt cggtataaac ttttacagta gaaagagagg
    31501 gcgttacata tttcgctaca ctgacatcat tgaaacccat cacagaaata tcttctggta
    31561 ccactaaacc tttttcttga atcgctttca gtgcgccaat cgctaatgca tcattagctg
    31621 caaaaagcgc atcaggaaca tgcggattcg tttttaaata ctgcttcact gcttgatatc
    31681 ctgactcgac agaaaaatct gcttcgataa ttggaccttc gtcgtagcct ttttctgcca
    31741 aaacttgttg aaatgccata attctgggat cttttagccg ctgatggttt ttcttcgtat
    31801 attctgttcc agcaagcata gcgatatgtt gcgctttatg ggagacaaaa tgttcaatga
    31861 ctttttgaac acttgactta aagtcgacaa ctagagagtc gattccttga gaagtgccgt
    31921 cagaatctac caaaagcaag gcattttggg atcctttcat ttctaataac tgagcttcgt
    31981 cgaattttcc tagtgcgatc gttccatctg tcggaagatc actaatggaa tcccacgttt
    32041 ctttcaataa agtaacgttt gcttcttctg cctttttttc gattcctaga cgaatagata
    32101 aataataaag atcttcaagc tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt
    32161 gcttagtatt tgctgcattt ttacgatgtt tcgtataatt taagcgttct gctgcttcaa
    32221 aaattttttg ctttgtggct aagccaacgg aaagatcagg atcatagttg agaactcttg
    32281 aaacagtagc aggagaaact cctgccaact gcgcaatgtc tttaattgtt gccatttgtc
    32341 tctcctcctt ttttacgttt ataaaattgc agtaaatcgt tcaaacgctg cctgtccttt
    32401 ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt gcaaaaacct gacctacaga
    32461 tttttgtacg acatcggtga ccgtatcttt cgtcaaatca ggatgttctt tttttaattg
    32521 ttctgcccac ggacgatgat attcagccat ctggttttct tctcctaata agaaatgctc
    32581 tacttctact agttctggtt tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat
    32641 caacccaatg ttctcctttt tgatatgttg gacatcttta tgtggatgaa aaatcccatc
    32701 aggatgttct tctgaaacat tgttgtctcg aaggacaagg tccaactcaa agtaattgtc
    32761 ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta ccatctgctg aaaaagctct
    32821 gatagatact gtttcatccg aatatgcttt ccacttttcc aatactgagt tagctgcttc
    32881 aatcaacaat tttttctctg ttgattgcaa acggatcaca gacatcggcc atttcacgac
    32941 tcctgcgatg acttgcggat aatctgctag tttgataagt ttttctatcg gtgcttttgc
    33001 cataggaaat tcatgacgtc ctgcctgata atgatcatga gaaagaatcg atcctccaac
    33061 aatcggcaag tcagcattcg aaccaacaaa gtaatgaggc aagacttcag taatccgaag
    33121 cagtctagca aatgtttcct ttgtgatttt catcgggcgg tgttgttctg ataaaatgat
    33181 cgaatgttca ttgtaataag cataaggaga atattggaat ccccagcttt caccgtctag
    33241 attcattcga ataatccggt gattggtgcg ggctggatag ttcaagcgac ctctatatcc
    33301 ttcgttttcc atgcaaagca tgcaggtagg atagtctact tttaccgcct cgcgttctgc
    33361 tgcgatttgt tttggatctt tttctggctt agataaattg attgtgattt ccagttcacc
    33421 atactccgac tctgccggaa atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc
    33481 atttttctta cttaaattaa agaaatactc agttgcttcc tctgggcttt tcgaatagtg
    33541 ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc
    33601 ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca ccattttctc ttgccttagc
    33661 gacaagctga tccaataagt ctacagattc ttccttaact ggtcttactt caatgctttc
    33721 tagagcatct tctcctacca tccctaaaag acgattctgt aaatataagc ggtccatttc
    33781 catccatccg ccagcttcga tcgcaagtgt ggcaaaatca caaatcgttt gactgattga
    33841 catatctcat tcccctattc ttcataacca tttggatggc tgacatgcca atcccatgct
    33901 gttttgataa ttgctttgat atctgtgtat tcaggtttcc aacccagaac ctctctcgct
    33961 ttttcacttg aagcgactaa tcggctagga tctccagctc ttcttggtgc tatttctgct
    34021 ggaatttctt ttcctgtcac ttctctagct gcttctagca tttccttgac tgagtatcct
    34081 ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt ctttcaagta ttccaaagct
    34141 aaaatatgtg cagcaatcaa atcttctact tgaacataat ctctgataca tgttccatct
    34201 ggtgtatcat aatcgtctcc ataaacagcc aatgcttttc tttgaccaag agctacttgt
    34261 aaaatgattg ggacaagatg cgtttctggt gtatgatctt ctccgataga tgcatctgct
    34321 tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc gcatgccata tgcttggtca
    34381 caccacttca tcattttttc catcatcaat ttactttcac catacggatt tttaggatta
    34441 gctggtgtat cttctgtgat tggagactct tttggttctc cgtaagttgc tgcagtagaa
    34501 gagaacacga ttttattcac attgaactcg tgcatgactt caagtaagat ttgcataccg
    34561 tatacgttgt tgttgaaata catcaatggt ttttcgacag attctcctac taaagagctt
    34621 gcagcaaaat ggattactcc ttcaatcggt tccttttcaa atacggaacg tagaaattct
    34681 ttatcccgga tgtctccttc ataaaaatga gcatctggat gtactgcttg tttgtgacct
    34741 gtgagcaaat tatctaccac agctacttga tacccctttt gaactagctg gtctacagcg
    34801 tgcgaaccga tataaccggc tccgcctagt actaaaattg tcataaaaaa cactcctttg
    34861 attagtaaaa ttgtgtttgt tgattatata taatttaaaa aaacgaagtt tacttttatg
    34921 gtgaaaacaa acaaagtaaa atttcgtaaa ttcatatggt aaatttataa taaaacattt
    34981 agtaaacatt ttcaaggatt ttttgcaaag gtttacaaac aaacgtaaaa ctagcctttt
    35041 tttctctttt gttccataat agatataaca aataataaat gaggtggatt caatgaaaaa
    35101 atttgtatct ggaatattag taggaagtct ggcaacagcc gctgccgttg ctggtttggt
    35161 cgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa tgatcgaaga
    35221 aaaccgtaaa aaagcaatgc gcaaacgcgt ttctcgttaa taattattta aaaacacgca
    35281 tgaagacttg tgaaagaaca gataagtcat catgcgtatt tttatttgtc agtcaatata
    35341 agttgtgcta ttttatctgc ctgtctcaga ttccatggat ctgtataagg tctagctact
    35401 gctgcagtta gccaatccac accttcaaca ggtattccat agcaataaaa gtcagactgg
    35461 atttcccctg aagcatcaat cacgcgattc gtacctcgct caacatctat agcttctgaa
    35521 tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt ttccttcaaa
    35581 ttttttatga caggattact tgaacgagaa aagcttgttt gtggcaatcg agcttcaatc
    35641 aaatagcgac tgctgatttc tttatcttgt agagaaataa catagcggtt gtgcttttgc
    35701 tgaatatcaa atggcggttc gaacaaatga aaaatacccg catctatcag tgctgctaat
    35761 tctttcgtcc gaatgactgg cggaccgatc gttaaaaagc tatttagatg agtaaaccat
    35821 ccccatagta tttctttgta ttcctttgca gaaaaaacgt gccattcaat agcctgccgg
    35881 attggatcac gccagtcttt taatgcatca aatgttgaag caaatgctcc tgtgcaattg
    35941 cctttttccg cttcttttat ctgcccttca ataaatgctc ggctagcttc tgcaaaggac
    36001 tcatcctgat aggtaagagg aatttctaaa tacgaccaat cccatctata agggattagt
    36061 gctggatgct ttccgagaca ctcctctgaa gagagggaga gaaaatcatg ctcaaaattt
    36121 ttctttggaa tcggcaaatg atattcttcg atgattttct tgtaataaaa caactcaacg
    36181 tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg atgccattgc
    36241 tccaggtttt cttttgttag taaccgtggc catgccattg atccgccttg cttttggttt
    36301 cttcctcgag ggtaataagg gagccccctt cttgatccac tgtatacaat cggttccttg
    36361 cctgatggat gatagacaag ttttcctttt tcgttctcga agcgcccttc tcttgatact
    36421 gtaaacaatc cgacataatc aaaaaaactt aagcctagac ctcttaaaaa gacattttct
    36481 ttaggcggta tcttcgaaag atcgacatct gcaggattag aaggaagctg atacagtaaa
    36541 tcatttttct ttgcgtagtc tacccacact ctttcttctt ctgtctcatc attttcccag
    36601 tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt gctgatctgt ctgtaaaaga
    36661 taaccatctt ttcttttgct gacttttttt actaaagaat aaacaaactg aatagaagct
    36721 ggaaattctt cttgcaactt tgagaaaaac cagcgttgat agagtccgta taaacatcga
    36781 gtagattgct gatctttttc cagacgattg atttcttcta taattagttg cttatttttg
    36841 gttaccgtct gttcgatata atctgtagca ggtcctttac tccattgaaa caagttgggt
    36901 ccctgtgtta ccaatcctcc actggacaat gtttcatctg taaaaagcgt cacttgttga
    36961 gaaacagaat tcattaataa ctccggtggc tgattcaacc gccaaactcg acctccagga
    37021 ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt atggagaatc
    37081 ctttctgcta tacttaatcc tctaggtccg gcaccaatga tccctatttt catttgcatg
    37141 cctccatttc gataaatttt ttcaataaaa cagcatggtt ctgatgttca ttttttgcac
    37201 cataaacaaa agtaatgact ggatgttctt tcataagttg actgagttta aaaatagctt
    37261 gcttcttttc ttcttcgaaa atcaattctt gctggtattt ctttgcaaat gaagcaaatc
    37321 gttctggtac atggtcaaaa gttttgcgtg cttcattact aggtgcaatt tctttcaacc
    37381 acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg ccagaaccta tccacgagta
    37441 ttctatagcc atcctctttt tcagcatcaa ggtaaacacg ttttaatttg atcatagttg
    37501 tttccttctt tctgcctttt cgcttagctt atatatgtag ataatagcac aaaataaaaa
    37561 aaatgaacgg cacaactatc cattgcgccg ttcatgtgat cttggtcaaa atctaatgat
    37621 tgattcggtt tttaaagctg tcatacaatc atctgtttta tactttttta cttttgattt
    37681 ttccagacca tccgtcatat ccacccttaa gtaaataaat ctcttgataa cctgctttac
    37741 gtaatttatt tgctgtacgg atgctcaagc ttttcttttg atcgtaaata tagacaggct
    37801 ggtcttttcg aatagatcct aatgaatctt tcaaaacagt gtaagggatg tttcttgcac
    37861 ctaaaatatg cccagcatca aacacatctt tttcgcgaac atcgattact tgtgcttttc
    37921 tcattccttc tcgaaattct tcttctgtca aagtcgttgc tgaacgcttg gccattactc
    37981 gtagatagac ttcccaaaag atgattgcaa gcacaatcaa tagcaaaatg atattaatta
    38041 cccataaaac catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta atgaggcagc
    38101 accaatgaca ccagcttcat taccaagctc tgctaatttg atttttgttg attctcttac
    38161 ttgcgggaaa gtaaattctt taaaatattt ctctacacgg ctacgtaaaa attctcctgc
    38221 tgccgatact cctccaccta acacaatact tgacggattc aatgtattcc ccaagttggc
    38281 gcaagctaaa cctaagtaga agcatacgcg atcaacaacc atcaatgcaa acggatcgtc
    38341 ttcttgagcc atttcaaaga tatctttact tgttacttct tcaccgttgt ctaacattgc
    38401 tttcagtttt gaatctcctg cgtattcttc tgccaagtaa cgacctagac gtaccacacc
    38461 agtagcgcta gacacagttt ctaaacatcc gcgtttaccg caagtacatt cgaacccttc
    38521 aggatccaca gtgatatggc cgatctctcc gcctgctccg ctcacgccgt gaatcaaatt
    38581 tccgccagca acgataccac cgccaacacc tgttcctaga gtaacgaata caacgtctgg
    38641 atcgttttct ccagcacctt tccatcgttc acctaaagca gcaacattcg catcattatc
    38701 gatagcgaat ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt gaacttctgt
    38761 ccagtttaag ttatacgcac caataaccgt tccagcttcg ctgtccacgc ttcctggggt
    38821 acccatgccg attccgataa agtcttcggc tttcatatta tataaattca aacgatggtt
    38881 gattgattca atgatttcag gaacgatatg cttaccatca tccaaaatat tggtatcgat
    38941 gctccatttt tgttgaatct ctccttctgg tgttaaaatt gcaaattttg ctgttgttcc
    39001 acctaaatca atcccaataa tcttcttgtc cattacaaat ttcctttctt tcggctttct
    39061 tcaattcggt gctctctttt taatatgcgc cacgctgtca tgtatgtatc atgatcaatc
    39121 aaacggctat catacagatt acgcaattcg atagtcatta attcaatgtc atagatgcgt
    39181 gcacccacat agataaagat gccaaatttt ttgaacagct gctgtacatc atataagctc
    39241 tccatcttta agcacttcct tcatattata caagtaaacc atattttttt aaacccagta
    39301 aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt tggtgtatcg
    39361 aatagcttgt ctgttgtttc ggtagaccca aaacattccc taacactaat ccgccaatta
    39421 gcccaccgac atgtccccaa acatctacag aggtatccag aagaccaaaa acaaatgtca
    39481 acacaataaa taaaagaaaa tgtctgacta gctgctggat cgctgggtta tgtttaaaat
    39541 gaaaccctaa gataaacaac gcaccgaaca aaccgaatat cgaagtactc gcaccaccag
    39601 acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc ccgctgaaca
    39661 tataaatcaa gaaaaaacgc caatggccat agattgcctc gatttgctgc cccataaaat
    39721 acagtatcag tgagttcatt acgaaatgca tcagtccaaa atggatgaag attggtgtca
    39781 ctaaacgcca ccattcattg aagtgaacaa gataaggacc atacattccg gcataataag
    39841 ggatctcaag tcgcggaaat aattccatca tgaggaagac cgctgtctgt atccctaaaa
    39901 aagcataggt ccataacggt cgtcttaacc atgcacgcaa ttttatttgc tgttgataat
    39961 tcatgaaaac ctcccatttc attttagtca ataaacaatt gttggatctt ctgatcaaag
    40021 ctctccggtt gccattgttc attcaattgt tcacgaaaaa caaggctaca tgttttttta
    40081 tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg ataaccggct
    40141 ctattgtaga taatacctgg tacaatgagg agatcgatca tctccggcaa atagagcttc
    40201 tctgattgtg gctcaagaac accaaaactc gaacgtacaa gaacggactg ttcatccatt
    40261 tcataaaacg ccatctgacg tttcggcaaa gttttaggta caactatttt cttttgttct
    40321 ttttctgctc gttggataac cggctgtgta tccagttcaa attcattcga gaaagtcgtc
    40381 cctatcactt ttgctgactt ccagtactcg ctggcgaata gctgtttata gattcgctgt
    40441 tctttttcct ttttcttgcc acttttggcc agatttttca gcttttcgat tcctttccgg
    40501 cgtaatctta ctttcaattg gtccaaaaac tcacccttcc ttctctcata aaatggtata
    40561 ggaaaaaaag aaaaatagca atcgaacttt caaataaatg cataaataga ataaaaagaa
    40621 gacctagaaa tgattgacct tcctttttta atgaatttca actgttcagc gcgtgttttt
    40681 gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc aaaattcctg
    40741 taggtacttc cccacttggt agaatcactc tagcaaccat atctgcacta agcacaaaaa
    40801 tggaaccgaa caaaccgctc agaacaaagg tccagttatt ttctttacgt acgactagct
    40861 tcgcagcatg aggagctagt agtcctacaa aagataaact acctgtcacc gctacactac
    40921 cgcttgccaa agcaacagct aacaacaaaa ggattttttg acttttagcc acgttaaggc
    40981 ctaatccttg cgcgcgttct tgaccaaatg atagcaatcc aatcctctgt gcataaagtc
    41041 ctcctagcgg aattaagata accgcccatg gaagcaatgc ccaaacataa ggccagctcg
    41101 caccccagat tgatcccgct aaccatgcat tcacaaatcc ataattttcc ttagataatt
    41161 ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt aacggcaata ccggacaata
    41221 agattcgatt catacccaaa aagccatttc gatcataggc cgcaagatag acacagcaag
    41281 cagctaaaac accacctaga aaggctacaa ctagcaatag aaacggcagc gaatagtgcg
    41341 aaaagaaacc taaataaagc atcactaaaa atcctgctcc ggcattaatg cccaatatac
    41401 tagaatctgc caattcattt cttgttactc cttgcaagat ataaccagaa acgccaagac
    41461 aagctcctgc taataaactg atcagtaatc gaggaactcg aaattctcta acaatcagcg
    41521 ccaaagaaga tgattctttc cctatgaacg tggcaaaaag ctgatccagg taaataaaag
    41581 gcgtaccaat cattaaacta cctatagaca ggatgacaag tacaaaaagc aagaaataaa
    41641 acgtccaata tatttttttc accgttgttc cctccttact tgtatcagta aaaacggaac
    41701 gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa gggggattga tcatcctcgc
    41761 tgtaaaatcc gccaacataa ccaatccacc gccaactaaa aaagagacag gtagaacata
    41821 acgatagtcc gttccaacca ttccacgaac aactggcgga accaacagtc caataaagga
    41881 gactggaccg actaacgaaa ctgctatggc tgccaacact gcgactagac agaaaacaat
    41941 caaccgcgtt cgtttaggat gacttccaag agctaatgca tgtgtttcac tcaaatgaat
    42001 caaagatatt gcttttccta atgaaaaaga aaccaaaaca gcaagtatat ataaaggaaa
    42061 caatactgct aattgtttcc atgtgatatt ggcagctccg cctacaaacc aaaaagcaag
    42121 atcctgctgc agattaaaga attgggtcag tgcttggctg atagcagtaa aaaaagaact
    42181 aatagctacg ccagctaaaa tcagctgtaa aggattcatg cccattttcg aaaactgcaa
    42241 aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc aatgacatta gaaatacaga
    42301 aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc attgccaacg aagcacccgc
    42361 gttgattcct agtaagcctg aatctgctaa aggatttctt gtcactcctt gcatcaatgc
    42421 accgctcaat gcaaaacaac tccctacaag aaatgctccc gccacacgag gcaaacgaat
    42481 atttcgtatg atttggtgac tttgttttga tctatcaaaa tcaaacaacg cctgccaaaa
    42541 atctgctttt cccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa taatcaaaag
    42601 aacaaaacag accaaaagca ggagtctatt tttcttcata gtcctcataa ttgatcctcc
    42661 tgctcttttg gcaacaaatc ataggttaga aaaactggtt tatttgttct tggaagcgtg
    42721 acaatcgttg catcaatctc aaatattttt ttcatacact cttttgtgat cacttcttca
    42781 ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta ataaataatc tgaaaaacgt
    42841 gaagcatgat taatgtcatg aatcgacatc aagatcgttg tttgtttcat ttgattgatt
    42901 tcagtcaata actgtaggat ctctaattgg tgtgccggat ctaaaaatgt tgtcggttca
    42961 tccaagatca agatatctgt ttcctgagca agcgccatag cgatccagac acgctgggtt
    43021 tgtccaccag acagcgttgc gatatcccgc atacgaaatc cagctagacc tgtagcatcc
    43081 aacgcccact cgattgcttc atgatcttgc gtgtccatgc cactgaacag tttttgataa
    43141 gggtatcgtc cgtaagatac tacttcttct actgtcatac ctgtagggtg ttcactagtt
    43201 tgtgctaata atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga gatatcagag
    43261 ttattgacaa tgatctttcc tgatttcaca ggtagtatac gggtaaaact tttcaataaa
    43321 gtagattttc cacttccatt tggcccaatc aaactagtga ttttcccttc aggaatcgta
    43381 acagacatgt tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc agctgctctt
    43441 aatctcgtca tttcacccat cctttcgctg aaaatcattc tcaattatga cgctgtttca
    43501 acatttcgtc aagcgctact ttcatatcta tagccaaatg aagcggttga cagcgttcga
    43561 aatatcagga ataataacca atgagaaaga ttctcaatta tgtcaggagg aaagaaatga
    43621 aattaaaaac aaaagtattc acattatcta gcatattgct gctggctgga gtactgagcg
    43681 cttgcttccc ctcttcagat aaagaaacag ctacaactgc caattcgacg acttcatcca
    43741 atagtactca tacagtcaca gatacacttg gacataaagt agagattcca aacgcgccca
    43801 aacggatcat cggcagttac ttagaagact atctgattgc actaggggaa aaacctatcg
    43861 cccagtggac tgttggcagt ggttccattc aagattatct gcaagatgat ttaaaggatg
    43921 tcccaacgat ttcttatgac cttccttatg agaaagtgtt aagctttgaa cctgatttgc
    43981 tgttgatttc ttcttccgct actgtcgaag gcgggaaata cgaacaatat agcaaaatcg
    44041 cgccaactta tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa ctaaaagatg
    44101 tcggcaaagc tttggcaaaa gaaaaagagg cagaaaaaat catttccgat tatcaaaaat
    44161 ccatcaagaa aactcgagaa gaactagcag ataaaatcga gaacaagaca gctgcagtgt
    44221 tgtgggtgac caacaactca gcctttatgg tcagcaaaaa tcggtcaagt ggaagaatcg
    44281 tatacgacga tctggaattt ggtgttcctg atctagtgga agaggtctcc aaaaaagcca
    44341 cttctgactg gtctgctgtt tcctctgaaa agctagctga attagatgct gactacatca
    44401 tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga accaagctgg aagaatctta
    44461 aagcagtgac agacaatcat gtactgcaat tcggacctga atctagctgg ctatataacg
    44521 gacctattgc cagtcaggaa atggtcaatg atatcaaaga aaaactaaat tgattttaac
    44581 taaaaaaagc atcttcacga ttagtcgtga gatgcttttg ttcttattaa caaactagac
    44641 gcatagattt tattcttctt ttgaagcaac cctatttttt actctttccc atttttgaaa
    44701 acgatgggca tatagatttt cttgatccgt tatcccctca tactcctcta taagggcgaa
    44761 gtctgaaaaa tcaatattcc caatatacgt atcgccttca aacgtgctat cgatcaatgt
    44821 tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa atccgactac cacccgaaac
    44881 atagatatct tctgagatcg tttgactata cgacaaaagt tcatcaatcg aatgcattac
    44941 ttccgcattt tctttcgtac ggaaatttgg ttgcgttgtc aacacgatga tatgacgata
    45001 aggaagtgaa agatccccca ttccttcata ggtctttctt cccatcacta aaattcgatc
    45061 cattgtatgt tcgcgaaaga aacgcatatc atttggcaat cgccatggta acaaaccatt
    45121 tttgccgatc agaccatttt tatcttgtgc ccacatggaa ataaacatat tatccgctcc
    45181 ttatgatttt agatgttttg agataaaaaa agtctcaatt ctttgaccag caaggaattg
    45241 agaactttaa aaacattatt ttgtttcacg gtgcaaagta actttgcgtt cgcgtgggca
    45301 atatttttgt ttttccaaac gatcaggatt gttacgtttg tttttgcttg ttagatagtt
    45361 acgttcttta caagaagtac actctaaagt gatgtttacg cgcatgattt tccctcccat
    45421 ttttacaaga atcctctaag atgactctca gacttgatta tcttaccatg attttttcca
    45481 gattgctagt gtttttcacg attttgttaa gtaaaactac ttgacgataa atacaacggt
    45541 tttacttctc agacaaaatc tttttctttt tgtataaata cccttacact tgtttataga
    45601 gactatgttt ttttaaataa agggggatac taatatgcca tggaacatga aggactttcc
    45661 tgcatcaatg aaaaacttag ataaactgac aagaaaaaag gcgatcgaca tcgcaaatgc
    45721 tctattagac gagggttacc ctgatagccg tgcgatccca attgccattg atcaagcaaa
    45781 agagtggtat gaaaatgcta gtgaatcaga aagacgaaca ttcgaaaaag aaaaaaatcc
    45841 aagtaagtcg gataaacatg atacgaatcc tcgtgccgga aaattgcttg attcagatgt
    45901 gatcgtagaa tatgccgaag aacaatggat cgtgaaatct aaaggagcaa aaaaggctag
    45961 caaccatttt gatacaaaga aagaagcgat cgaaaaagga aagcaagtag cacaaaacaa
    46021 agaatcaact ttggtcattt ataaaaaaga tgggacaaaa gaaaaagaaa tcgattattg
    46081 aacatatgta aaggggttgt gacgaaaatc gtgtcacaac cccttatgtt cgaaacaatg
    46141 gtgttcctct gccaaatccg aaccacaagt cataatatct ataaaacggg aagagctgtt
    46201 ttttttaatt gtttcttatc actcggtgtt aaacggtagg agcaaaccta ttttttattg
    46261 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
    46321 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
    46381 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
    46441 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
    46501 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
    46561 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataag
    46621 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
    46681 acttccaaag aacttgccat agaaagttta tctgccttca acaagatatc ttttggcatc
    46741 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
    46801 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgtcaac
    46861 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
    46921 ggagcaagtg attcatataa atggatccgt ccatggaaat ttttcatttt tccgatttta
    46981 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
    47041 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
    47101 ccttctccag attggactac ccgcacgctt tgtgaagcaa gatttgctaa aaagtacaaa
    47161 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
    47221 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaaatccaa tagttcggtt
    47281 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
    47341 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
    47401 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
    47461 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
    47521 tgctgaatgt ctagttttcc atctttatac gtataataat ggccttcagg caaacgatag
    47581 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
    47641 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
    47701 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
    47761 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
    47821 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
    47881 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
    47941 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
    48001 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
    48061 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
    48121 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
    48181 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
    48241 cggacgaata ttttctctct tacagtttcg tcacgtgaaa acaaaagatg aaagtttctg
    48301 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctatc
    48361 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa
    48421 aagacacgac ctaaagatcg tgtctaatta tgactgtatc agaaaatatg ataccaaacg
    48481 gatctattca ttaattaatt gacatagttt atctgttgct cttttatgac tggataactg
    48541 accaattcat gacgatccaa gtcttcttta ttcatattta cagcagtaat ttgactgtct
    48601 tcatacgtac gataataata aatcccttta tcaacgttac agcaagaaga ataaatcgta
    48661 tattcgtatt ttccttctcc agtatcgcat actccttttt gttgctcgac tgagcctagt
    48721 atatgaaaaa attgactgat actttcagat tctgaatcac ctgatgcagc attcatttta
    48781 gtaaatgtgg cttttacaaa tcgagaaaca gaagagagaa ctccaggtaa gcccaatcct
    48841 cccatcccac gactataaac atccaagctg atctggtttg aaaaatgatt ttctggtgtt
    48901 ccatttgata gcgagcgata attgttcaga ttaaataatt gataatcaaa tggtggatta
    48961 tttgtcaaca caccgacagg attatcatat aaatgaagtc catctttcat actttcaatt
    49021 actatcgatt tttctttatc agctaataac cagtgtaaag gtgacaaggg tagtttttca
    49081 ctaaaatcga cattgactag attgatagta gccaataatt ctttagcttc gtctatggtt
    49141 gagcattgcc ctagtatcca tggaataaac tcaaatggtg caacgttatc ttttccttct
    49201 tgtaattctt tataatcagc attgccagga aaattcagtc ctgccatact caatcctttt
    49261 tcatttgtag cctcataata gagtggatag ttggctaccc cagctgctat cccaatcatt
    49321 gcataatgtt tatctaaatt ttttaccttt ctaaaatcga aacgataatt tcttggagta
    49381 atagtgacta cttcattata agacatctca taatcaaaat tccttccaaa ataatggtct
    49441 ttcgtcgcat aagtgattgc ggtacacatc gttttccctc ctaaaacaaa tcgtttttac
    49501 acttttatct tacatctagt attttttttt atcaaaaaaa aggccaaaga agattgattt
    49561 actagcattt ataaaaaata gtactcaggg ggaatcaaaa aatattgtag gctaaaagta
    49621 caacaaaaat aaggggctgt gacaaaagcc ttgtcacagc cccttattcc gaacaaacgg
    49681 tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaaata ggaaaagcta
    49741 tttttgtttg gccgttctta ttacttgatg caggacagct tctttacaac ctctaatcgt
    49801 tgatataagg aaggtacagg atttgaacct gcgcgccggt attagccggt tcgccggatt
    49861 tcgagtccgg tgcattacca ctctgccaac cttccgtaag tactattttt aaactttttc
    49921 cacgaaatgt caagcctatt tctaatagat aaataacaga aatcgctttt tttagcaaaa
    49981 aagtccattg acagtttgca gccaacggac ttaccgatca aattcatact gatagaacac
    50041 tatctgatta tttcaggatt atctaatgct aaaatcgagt caattgtccg tttgatcagt
    50101 gcaatgattt gttcttctgt tggatgcatg atatttgatg acatcaatga cgctaagcca
    50161 gtcactgcta cccacgtacc agtatgcaga gcatcgacat attcatccga taattgattg
    50221 tatctttcat cttctttaac tatttcacag aaataattgt atgaaaaatc atgcattttc
    50281 tttccgtccc catgttcatc aatgtacaat gccatgtaaa gactatgatg cttttttgca
    50341 aagtagatat agttcaaacc gagatcaatg attttatcac cagtacgttc aatagggaaa
    50401 acatcttctt ttagttgttc aaagatagaa tccagcaacg tattttttaa atcttccatg
    50461 tttttgaatt ccagataaat cggctgtgtc gaaatcccca tctttttagc aatatttctc
    50521 gcagtaaatc ctttaaaccc ttcctttgca acaacatcat aagcagcttt tagaatttga
    50581 tccttggtat agacttttct tctcattaaa actcccccac ttctttactg cagtatatgt
    50641 tgttttattg taccctattt ttagcatttt ttgcaaacat tttactcaaa acgttatatt
    50701 ttaaattttt ttctcagtct tctttaactt tttttgcaaa attaaaaaaa tttggttaaa
    50761 ttcaatgggt tcccttacat ttttagtttt gacctaaatg atagttgacc aaatctttca
    50821 tttcaagtta tagttaaaga aaatgaaaaa gaaggtgtgt aatggaaaac gaaactgacg
    50881 ttatttatat tcatcctcaa aaacgaatcg tttcacagaa aagaaagtat ttttatcttg
    50941 gttttacagg tgcttttttt ctttttattg gcttgctttc tggtactcct actgataatt
    51001 ggagtggatt gctaactatt cttacctcgc caagcaatct tttgacagat tattttgatt
    51061 taggcggttt cggaagcgca ttcataaatg tagggatttt gaccttactt tctgtgcttt
    51121 tagcttatcg gcacaaggtg attttaaacg gtcccttatt tgcttcgatt ctaacagtta
    51181 ccggtttctc tttttttggc aaaaaccttt ataattctat ctcaatcatt ttaggtgtgt
    51241 atctatacgc tgtatttgtc aataaacctt tttctcagta catcatgatc ggactgtttg
    51301 gtagcgcatt atcaccggtc gttagttata tcacttttgg tatgcgtttt cctttactga
    51361 taggtatttt attggggaat ttagctggca tagcaattgg tctacttctt ccgcccttag
    51421 ctgctcaaac tttagttttc catcgtggat tcacacttta taatattgga ttcacctctg
    51481 ggttgattgc gatgactttt actgctgttt tgaggttatt cagctattca atcgtcgaaa
    51541 atacattggt ttccaatgaa tatcactttc ctctcatttg gattattttt ggcttttttt
    51601 cgctgacagt cggaattggt ttctattata attcctttcg gctttcgggg attcgagaga
    51661 ttttcgattc atctggaaaa ttgacaactg atttcattgc taattctggt attggtgcta
    51721 ctttgatcaa catgggctta gttggtctga tgctgtcttc ttatgtttta ctggttggcg
    51781 gtcagttaaa tggacctgtc atcggtgcta ttttatctgc ggttggtttc agtgcttttg
    51841 gttgtcattt aaaaaacagt tttccgatac tagtcggaat atttattgct tctttgtttg
    51901 gaacttttca tgagatcacc agtactggca tgcttgtggc agctgtgttt gggacaggtc
    51961 tcgcgcccat aagcggtttt tacggcagtt tttacggtgt aatagctggt gtgctacata
    52021 ttgctcttgt ccataacgtc agcactttac acggaggact aaatctatat aacagtggtt
    52081 tttcaactgg tttcgttgca ggtatccttg taccgatact agataacttt accgctgtca
    52141 gaaaggaaaa aaagacactt ggaaaaagaa ttatcaaaaa gaatcatcga tgagcttctt
    52201 acttatttat attcccatga aataactgaa atacgtattg gagtagaatt tgctgaggaa
    52261 gggcttttca tagagataca gggtacggca gaaactcagc cagaagattt agatgtactt
    52321 gctgatttgc tgaacaatcc tcgggatcct agtatggaaa tctactatca ggaactatta
    52381 ggtaatcccc gacatgatcg ggaagattat catctgttag gtagtttgat tgatgacgtg
    52441 gaaatcatgt acgcccagcc gattatcaat attaagatct ttcgacgtgc ttctcctcgt
    52501 tcctattatt aaccctattt tctatttata aaaggctgaa tcaaaaaagc agctcctgaa
    52561 agttcgatta aaatctactt tcaggagctg ttcaagcttg acattattga ttttccaatc
    52621 cttcatacac ttcatctgtt acaggttcta aaaactctga gtttccggaa gtgatcgcta
    52681 aatgttcaaa ccaactgtct tttgtagcac catgccagtg tttgattcct ttgtgcgtca
    52741 ccacaacgtc tccagctctt aatcgtcttg ctggctttcc ttcttcctgg taccatcctt
    52801 ctccgccagt cacaagtaag atttgatatc catctgtatg gacatgccag tgattgcgac
    52861 aacctggttc aaatgttaca ttccctacac caacagaaac ttcgggatca ttcactaaca
    52921 tattcagata gctttgccca gtaaatacct gagcgtaagc ttcgtttttt tcccctgctg
    52981 gaaaaatcac gccattgtgt atttcttcgt attttgtcat catttacgcc tctttcttgt
    53041 ttacgctgtt tccttctaga tagatttctt ttgctaagtt aaaagctgac caagcttttg
    53101 gccatcctac ataaaaagct aaatgggtaa tgatttctgc tatttcttca ccagtaatgc
    53161 cattttgttt gccaatcttc agatgagctg gcaattgttc aaaatttcct cctgaaatta
    53221 ataaagatat ggtgatgata ctacgctgat gagcagataa ttcttctctg gaccatactt
    53281 gtccaaataa aacatcatca ttcaattctg caaattttgg tgcaaaatct cctaattgat
    53341 ctcgtcctgc ggtttgtttt tctgccattt ctcttattct cccttcacga ctgttaggtg
    53401 attcaactat ggttctaagg caactttatt ttctattgtt tcttcgcatt tcattttaaa
    53461 tgtagtcttt cagcctgtcc aatgcttagt ttattcagtc gatatgcttt ttaagcatag
    53521 ttgctaacgc tttttttatt taaaaaaaaa caagttatta aataagtctt tagttggatg
    53581 tttttttgag atcaatcttt tatacttatg atgaacttac aaaattgttt gttttctgtc
    53641 atatcaaaaa atggcgcagt tagttgcttt tctagtaaag tatagttaac aaagaacctc
    53701 taaggagttg atgaataatg aatattgctg cctctcgaaa actgataaat ttcatatcca
    53761 ttatcggatt aggcctatca atcgcgctga cgatttattt tattaattta ggtgttttca
    53821 aagatttgaa tgcgttaaga ggattagtag gtgattccat cattttagga ccgattattt
    53881 ttgtcttcat ccaaatcctg caagtagtta ttccaatcat tcctggaggg atcagtactg
    53941 cagctggtgt cctgatattt ggcccttatg ctggatttat ctataactat attggaatct
    54001 gcattggttc gattatcatt tttctgctag ggcgtcgtta tggaaagcca ttcatacttt
    54061 ccatgattag cgataagaca tataacaaat atattggctg gctggataat caaaaccgct
    54121 ttgagaagct gtttgctcta gctatttttc ttccagttgc tcctgacgat gcgttatgtt
    54181 tgatggctgg attgacaaac atgtcagtga aacgatatac actaatcatt ttgattgcta
    54241 aaccactgtc gattttctta tatagtatgg ccttgattta tggaggccaa tatctaagtg
    54301 gtcttcttgg ataatattta ttttgcaaaa ttagtcattg attgacaatg ttaaatctgg
    54361 gaaaggggct gcaaaacaca ctgcaccctt tcctttttca ttagatttgc gctacaatgt
    54421 gaagagcagg tgataggatg aattatttaa aacgtagctt aggtaatgct tgggactttt
    54481 taaaaggaac acaagcttat ttccgtgatg tcttgctgat gcatggcttt attttattta
    54541 tttgtttacc cttattgagc agtacgaccc gttttatttt acaccgaggg gcaattgact
    54601 atttgtctta tgacaatatt ccttcaattt tttctcaaca tcctggagtt ctactctcgt
    54661 taatcgtagt tttgatgatg attttactgt tagtttattt cgaatttact tttcttttga
    54721 tgagtgtctt ttttattaaa aagaaagaac caatctcttt gaagcaactc ttacacttga
    54781 cgatacttca gctgaagaaa gtgagaccca ttactttttt gtttttcctt gcttattttt
    54841 tattgattct accgatcagc ggattaagtt ttaattctga tctactgtca aaaataaaaa
    54901 tccccgcttt cattatggat tttatttttg ctaaccgttg gatcatcgtc agtttctttt
    54961 tactcgttta tattttcctt ggctatattg ggattcgact gatttttgct ttaccggaaa
    55021 tgattctacg agatcgtcct tttcgagcag ccattcggga aagctggtca ttgaccaaat
    55081 cgcgtctttt ggccatcacg ggccaattta ttgtgattgg cggtacgatt ttactccttt
    55141 cttctttagg atatatcgtt gttattttgg cacaaagtat agttgaacaa tttttcccag
    55201 actatgcatt gatcagcgct gtgtttgcta tgacgctttt acaagggatt ttattattca
    55261 atatcgtgat gtctaccgta gggatttttt atatcattgt tgatttcatg gatgacgaag
    55321 gcttcttgcc tgagatccct aagtggttta tcccacaagc ccccaacttg cgtttctctg
    55381 cgctgaaaaa cactggtttg actctttttg cagttttttt cggtattggc gtctgtcttt
    55441 acaatatgga ttatctgact tcagctgttc agacaaaacc agtgactgtc tctcacagag
    55501 gtgtgagtac ccaaaatggg gcgcaaaata ctttagaagc acttgaaaaa acaagcagag
    55561 actatcatcc tgattatgta gagatggatg ttcaggaaac aaaagatggt catttcgtag
    55621 tgatgcatga tgcaaatctt cgtcacttaa caggggtaaa tggaacacca caggatctta
    55681 cattgaaaga attgacaaat cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt
    55741 cttttgattc ttatttggaa aaagcacata agctaaacca aaaattactt attgagatca
    55801 agacctctaa aaaagattcg gatgaccttg tcgaacgatt tgtccataaa taccgcaaag
    55861 ttatcttaga gaaccatcat attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa
    55921 aagaagcaga accaaacttt tatgtgggct acatcttacc tttcaatatt gttgggcccc
    55981 ctgtgacgcc tgctgatttt ctgacaatgg aatatagtac gatcaaccgt aacttcatta
    56041 attctgccca ccaagacgga aaagaagtat atgtatggac aatcaacgat tcagatgcca
    56101 tgagtcgcat gatgttttat ggtgtggatg gtatcataac cgatgagatg ggactactaa
    56161 atgaaagtgt gaaacaaaca gatgaagaaa tcacttattc agataaactg ctaaattttg
    56221 ttttgggagt cggctaatca aaaatcgtta atgaaaaaag tctttataca acaaaaaacg
    56281 cataacctgc caccgcaggt atatgcgttt tttgtttgac gctcataatg tgtgcttaac
    56341 tttctcttga ttccttcatc gcacgaacaa tagctaaaat agcaggaatt gcagagatca
    56401 ctgcaagaat aattcccatt ttccctgaac tttttctttt tggcatatct tctcgctccc
    56461 ttctgtcttt atttatatta taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga
    56521 aaccaattca gtaggtgcgc taatatcaca gaattttacg agcatgcgat acttgctttc
    56581 attgataagt tgatagtctc gttggtcata actttcaaca tacacgacag ccgtgttgat
    56641 atatgtacgt tcaatacagc cgattagttc atgagatact ccgatcggtt tgcatatata
    56701 agatcttcct acttctgctt ttaccatttc attccctctt ttctttattt ttattttgtt
    56761 tttctttatt ttataataac aaattacctt atatttgaaa acgtttttat tcattttttt
    56821 attttttatt ttgagtatct ttctgctttt tagataacta ttttttaaca cgatcttaga
    56881 cgttgtgaca aaaacgtttg tttatattcc tatgttgttc gtgcgttttt ttctttatat
    56941 tgctaagtta tttataaaga ggtgagcata tgatttatat tcaagcaata gtatttgtaa
    57001 ccttgatgat tgggctactt atcggattaa aagaaaatag ctaaaccatt taagaaaaaa
    57061 taaataagga aaatgataaa tgctttttaa ttgcatcaaa aaacgtacga tgctaaactt
    57121 ggtataagtt cgaaagttta aaggaaactt tcaagaacta aattacatta caaaggagat
    57181 gaattttatg acgtttacaa aaacaaatat gcaataccgc gtagcttttg atacaacttc
    57241 taaccgtttt atggcgattg atgcacaaaa cgaaaataac gttgcgtttg gtattacaat
    57301 cgaacaagca gtccgagcat taaacgaatc tagataagga gtgaaccatt ccaaatggcg
    57361 actgatgaga tctcggaaaa tcaataaaag acgtttgtaa cggtaatccg cgacaaacgt
    57421 ctttttgtat tcattacata tatttggaaa ttgtctctct ttatctctag acctaaacaa
    57481 aaacacaagt tctaatatca accatctcgt tttttatgac aattttgtta gctgtttcga
    57541 tgataatatt ccgttattat ctgtataata tttaatataa taaaaataaa aaaggagatc
    57601 tttcatgaaa aaaattttag ggttgatcgt tatcatattg attgctttta taggatggaa
    57661 aggttgggac tactatcagt ctacttatgt aggtaaagat tattatgcgg tagtcaaagc
    57721 tccaatgcct gcagaaactg atatcaaagc agataatggg gaagtaattg gaagaggttt
    57781 taaatacaac gtagatgctt atgctgaaaa cggtgaaaaa agacagttag atttcgatgt
    57841 cattacttct ggtgactctg ccaatggttc tgcttacccc gaaggaacga tcttgcagtt
    57901 gaaagcaagc gaaaagcgga tcatagagaa aaaagtaatt accagcgata aggtaccctc
    57961 atctgtaaaa gaaaaattag gcctatctta agctaagaat atatcgtaaa aagcactcga
    58021 agtgagtgct ttttttgttg attttagaaa acaaaaaaat agaatgggtc ttcgaagtca
    58081 ctttcctgtt cttccagtcc ttattagaag atatcaaaga ttttttaggt cataaagata
    58141 tttctactac acaagtctat gcccatattt ctcccgaagt gaaaaaaaga tcaatgaatc
    58201 aactagaaaa ctatatagaa gagcaaataa aaaagcactc aaattgagtg ctttttttta
    58261 agttcgctat cactaaaact atcactaatt ttatttagaa gcgaacatgt ttctatcact
    58321 gattttttaa atctagcgaa aaaaccttat cttacaggcg ttcgttcaat tctttggcaa
    58381 ggtcttcaaa gcctggtttg cctagaagtg cgaacatgtt tttcttgtaa gcttctacac
    58441 ctggttggtc gaatgggttg attccattca agtaacctga aatacctaca gcaatttcaa
    58501 agaagtacat tgtataaccc aatgtataag catccatttc tggaattttg actagcaagt
    58561 taggcacgtc accgtctgta tgagcaagca atgttccttc aaatgctttt gtatttacga
    58621 agtccacttc ttttccttgc aagtagccta gaccgtctaa atcttctgct tgttcaggaa
    58681 ttgtgattga tttacgaggt ttttcaacct tcacaactgt ttcaaaaata ttgcgacgtc
    58741 cttcttggat gaattgtcct aaagagtgca aatcagttga gaagtttgca cttgaaggat
    58801 agatgccttt ttggtctttt ccttcagatt caccatacaa ttgtttccac cattctgaga
    58861 agtattgcat tcctggttca tagttgatca acaactcagt tactttgcct ttacggtaaa
    58921 gaatgttacg cattgctgcg tattggtaag cttcgttttc ttctagtttg tcgcttgagt
    58981 atgctttgct tgcatcagcg gcaccttgca tcaatgcatc gatatctgca ccactaacag
    59041 cgattggtaa caaaccaaca gcagttaata cagagaaacg tccgccgatg tcatcaggaa
    59101 tcacgaaagt ttcccaacct tctgcatctg cttctacttt aactgcacct tttgctttat
    59161 ctgttgtagc gtagatacgt ttgtttgctt cttcttgtcc atatttctta acaagaagct
    59221 ctttgaatac acggaacgca atcgctggtt cagtagttgt tccagatttt gaaataacgt
    59281 ttactgaaaa gtcgcgatca ccgattactt cgatcaagtc agcaatatat gttgagctga
    59341 ttgaatttcc ggcaaagaag atttgtggtg cttttcttgt gttgctatct aataaattga
    59401 agaatgtgtg attcaagaag tcaatcgctg cacgagcgcc taaataagaa ccaccgatac
    59461 cgataacaac taatacttca gaatcagatt ggattttttt agctgcttct ttgatacgtg
    59521 caaattcttc tttgtcatag ttcgttggta aatcgatcca gccgcggaag tcgcttcctg
    59581 cgccagtacc ttcccgtaat tcgttgtggg ctgctgttac ttggctttgc atgtacccta
    59641 actcttgttc gttgacaaac ggtgctactt ttgaatagtc aaaatgaatg tgtgccattt
    59701 gttctcctcc ttataggttt actctacagg taatacttta cgtttttttt tattattttt
    59761 caagcaaata aacaaaatgc tgacaatatt tccaagaaag tttcattttt caaactaaac
    59821 cttctgctgc cattgctttg gcaacttttt caaatccagc aacatttgct ccaagaagca
    59881 gatttttttc gtcgccgtac tcggctgctg tttggcgaca cgcatggtaa atcgtttcca
    59941 tgatttgatc cagctgctca tccacttgct ctctttccca agtcaatcgc tgggcgttct
    60001 gactcatttc taaagcagat actgccactc ctccagcatt agcagctttt cctggacaat
    60061 accacacgcc tgcttcatga agaacagaaa cagcttttac tgtacatggc atattagcgc
    60121 cttctgctac gattttgatc ccattttcta ctaagatttc ggccaagctt tcgtctattt
    60181 cattttgagt agcacatggc aaagcgatat ccgctttttc tttcaatgtc caaacagatt
    60241 ctccagcatg ataaatagca gaaggacgtt ggtttgcgta ttccgtcaaa cgttgtctat
    60301 tgatttcttt gactcctttc aatagtttca catctattcc ttctggatca tagatgtacc
    60361 cgctagaatc tgaacatgta acaactttcc cgcctagctc ttgcactttt tcgattgcat
    60421 aaatggctac attcccgctt ccggaaacaa aaactgtttt ttgattaaat gaatcttttt
    60481 cttcgttcaa tagatgtttg acataataca ctaatccata accagtagct tccgtacgta
    60541 tcttacttcc ccaaaaatcc aaaggtttac ctgtcaacac accagtatca tattttttca
    60601 atcgtttata ttcgccgaac aaatagccga tttctctagc tcctacaccg atatctcccg
    60661 caggaacatc aatcgatggg ccgatatgtt ttgacaattc cagcatgaaa ctttgacaaa
    60721 aacgcatgat ctcattatca gattttcctt taggatcgaa gtcacttccg ccttttcctc
    60781 caccgattgg cagaccggtt aaactattct tgaaaatctg ttcaaaagcc aaaaatttta
    60841 agatacttag attgacacta ggatggaagc gcaatccacc tttataaggg ccgatagctg
    60901 aattgtattg gatacggtag cctctattta ctcgccagtt tccttcatca tcttgccaag
    60961 gcactcgaaa ttgaataact ctttctggtt ccgttaaaag ttctaagata ttcttttcga
    61021 tatattcggg atgagtgtcc aaaaaagaca taaccgtggg catgaattca tcgattgctt
    61081 gtaaaaattc ttcttgccct tgatcctttt gatgtagctt ttcttgaatt gcttgaacat
    61141 attctgttgc gtttgtcatc gttccacgct cctttgctta tcttccccca ttttagctca
    61201 ttttctcttt tggctcaaaa catttttttc tgttttcttt cttcagatat gctacacttt
    61261 ccttgaggtg agggaatgga aaaactgtat gatgtgatcg tagtcggcgc tggaacaagc
    61321 gggatgatgg cagcgatcag tgcagctgaa caaggcgcac gcgtactctt gatcgaaaaa
    61381 aacaaaaaag caggaaaaaa actactgatg acaggtggtg gtcggtgcaa tgttacgaac
    61441 aatcgtcctg ttgatgattt gattgcacat attcctggaa atggaaagtt tttatatagt
    61501 acgtttgcgc aatggaataa tttcgatatc atgaacttct ttgaatcaca aggcgtacat
    61561 ttaaaagaag aggatcatgg acggatgttt cctgtaacaa ataaatcaaa aacgattatc
    61621 gaagcattgc tcaatcgtct gaaagaacta gatgtgacgc ttctattctc tactcgagta
    61681 gagaaattga tccacaaaga acataaaatt tacggtatac gcacggaatt cgaagagttt
    61741 catgctcctg ccgttatctt aacaactggt ggacggactt atccatctac tggttctact
    61801 ggagatgggt acaagatcgt gaagcgagtg ggtcacacgg tgacgcctct ttatgcgaca
    61861 gagtccccct tgatatcgga tgaaccgtat attcaggaga aaacacttca agggctttct
    61921 ttacaagaca tcactttacg cgttttaaat aaaaaaggtc gtgtattgac tgaacatacg
    61981 atggatttac ttttcaccca tttcggtatt tctggtccgg ctgcactgcg ttgttcaagt
    62041 tttgtcaata aagaattaga gaaaacggga gaaccagtga cactttctct ggattgtttc
    62101 cctacacaaa ccaaacaaga gcttattcat ttattgacag aaaaaagtaa aaccaccaaa
    62161 aagaacttgg taaatgcctg gcacggttta ttacctgagc ggttgttagt tttctttctt
    62221 gagcggcttg aaatggatca tctgacagga caacaagcct ctcaaaaaca aattcaagat
    62281 ttcgttcaat tatgcaaaga gtttaagcta tcaatcaaca agacttttcc tattgagaaa
    62341 tcatttgtaa ctggcggagg ggtttctcta aaggaaattc atcctaaaac gttggagagt
    62401 aaaatcattg atggtctgta ttttgcggga gaattactgg atgtcaatgg ttatactggc
    62461 ggctttaata tcacagcggc tttcgctaca gggcatgtag caggaatgaa tgctgggcaa
    62521 agagcataat gtataaaaaa gaaagaagaa gactgggcaa acaacatccc ggcctccttc
    62581 tttcttttat acgtatgatt tattgtttta tatctgcaaa ttgtccgctc gttaatgcac
    62641 agatatcttg aggtgctaac tccatctgta gccctcttct gccagccgag acaatgattt
    62701 gttcatagct ttctgcaatt tgatctaaat aagtaggaaa tagttttttc atacctatag
    62761 gcgagcagcc accacgtatg tatccggtcg ttgcttccaa atcttttaaa tggagcattt
    62821 ctactttttt attccctgaa acttttgcta atttttttag attcaattcg gcttctcccg
    62881 gtatcacagc aacaagaggc cccgtcacat tgcccacagc aaccagtgtt ttaaatatac
    62941 gagattggct ttctggaagc tgttcagcga ccgatgacgc acttaggtgg ctttcgtccc
    63001 actcatattc gtgctctgta tagggaatct ttttttgttc gaccatacgg attgcattcg
    63061 tttttactat ttttttcttt gccaaaaaat atcacctcaa acttgttcta caacggttca
    63121 tttaataatt gaataatttg cttcaatgct ttgaccggta attgtcctgg agcagaagta
    63181 gaatcgctgc ttaaggaacc aaatgtcata acggaacccg tcgttccgcc agccagacga
    63241 gaaattttac caagatttcc catggacatc gtaataatag gcatggagac aaaagcattt
    63301 gcccgtctag tcaactccat caaccgcaaa acatctcgtt cattattggg agttactgcc
    63361 agttttccga tatcggctcc aaaatgttcc ataacattca atcggtaaag caagacaggg
    63421 tctgctggtg tttctttaaa atcatgacta ctgataatta aaggaatgtt taaagacttg
    63481 atttcctcta tcagtctttt tcctagactt tctactttga acaactcgat atcgaccata
    63541 tctactaatc ctgtgtgtat cacatgttca taaaggtcgc gatactgacg gatcgatatg
    63601 tcttgttctc cgccttcagc taaggtacga aacgtaaata ataacggcag tccatcaagc
    63661 atctcatgga taagaaccaa tgtttctgct acttcatcaa aagaaaaaac ttcacgataa
    63721 tgatcgactc gccattcgac taaatcggcc cctgcttcac gtgcaagaac tgcttcacgt
    63781 aatacttcct ctcggacggc cccgataaga ggcgcacaaa tctttggtct accgcttcct
    63841 atattcaatc cctttacccg aaccattctg catcgctcct tctaatggtt atttttaatt
    63901 tgataaacaa acactttcag ataattgcct tctaaaaagg tatccgtcgt ctggaaatct
    63961 tttggcagac gatataaagc aagttgttca aaagaaacat ttttggcatg gaattcctct
    64021 tcaatcattg tttggaattg ttctatcgag acattcgcag catttgtaga cgcaataagt
    64081 atcccgtctt ctgctaaaat atccagacta tccttgacca gttccccata atcttttgct
    64141 acgcgaaaga cttttttctt atttctggca aaacttggtg gatccaatac aatcatgtca
    64201 tataaaaatt gttttctttt ggcgtatttg aaatactcga atacgtccat aacaatgatt
    64261 ttttgctgat cgacatccaa accattcact tcaaactgtt cctgtgtttt ttttaaactg
    64321 cgttttgcta gatcgacact tgttgtttcg attgcccctc ccattgcggc agcaacagaa
    64381 aaggcgccgg tataactaaa catgttcaaa actgttttcc cagaagcgaa accttctgct
    64441 aatgatcccc gtacttcttt ttggtctaaa aagatcccag tcattaatcc ttcatttaaa
    64501 taaacggcaa atttgactcc gttttctttt acgatcaagg gttccggtgc ctcttttccg
    64561 taaatgaact ggctctcagg cagatttact gattcaaacc gaattttctc atagcctcct
    64621 atgatttccg ggtagatttc acgaaaagct tgaatcaata atccacgatg tgcatataac
    64681 gtttcgttat accaagaaaa aacagcatag cctgcataat aatcaataat cagaccgccg
    64741 atcccgtctc cttctccatt gaatatacga aaagcggtag tagaatcatc tgaaaaaaag
    64801 aaagttcttt tttcttttga cttcacaaaa agttcttcaa aaaaagattg atcaaatggg
    64861 atgttctgct ggctcaatac ccaaccgatt cccttattct gctttcctag atatcctttt
    64921 gcgataaatt ggttttgcga atcaactaat tccacccatt gcgtggtttc tttttgggga
    64981 tgagcaaggt cttctttttg aatcagtgga tatccttttt tcaatcgact gcttgctctt
    65041 ttttttactt gtactttcat tccttcacct gctttagctt tctcttccca agtataacaa
    65101 aaaacatagg aattgtctta ctgctcattt tttcatgaag cttaaagaaa accttaatga
    65161 aacagcataa acttctggtt tcaattgaca ctatattgtc atactagtaa aataaatgag
    65221 aataagtgag ctgtgtaaca tggtttcatg acacttctgt gattaagaag tgcagaaagg
    65281 aagattatct tgaaatatat aaaaacacct gcctttttga ataagaggct cggatttttt
    65341 tctttactag tcgttttact ttggttgaaa acgatttttg cctatttagt tgatttccat
    65401 ctcggaatcg aaagtggcat tcaatacttt atcttgttta tcaaccctat tgcaactaca
    65461 ttgttattac ttggaattgc tctttatatc agacgcccta gagttgctta tatcacgatg
    65521 atggtcattt attttctttt gacattgctt gttttttcga atgtcactta ttatcgagaa
    65581 ttcacggact tcattacgat caacacgatt ttaggtgctg ggaaagtagc gagtggtctg
    65641 ggagaaagtg ccattcgact gtttcgccca tatgatgtga tctattggat cgattttatt
    65701 atcttgactg ttctgctgat tgccaaaaaa atcaagttgg accctcgccc gattcgagca
    65761 agaatggctt ttgcgatctc tacattagca gttatgattt tctcaggaaa tctttttatg
    65821 gcggaagctg atcgtccaga attgctgact cgaacattct ctcgtgatta tttggtcaaa
    65881 tatcttggct tgaatgcttt catggtttat gacggtgttc aaacttacca gacaaaccaa
    65941 gtgcgggcag aagcaagtcc caatgatatg aaagaagtag aaaattatgt aaaagaacat
    66001 tatgcagcac caaatgacga tttgttcggg ctcgctaaag gaaaaaatgt gatttatatc
    66061 catttagaaa gcttccagca attcctgatc gattataaat tgacagatga gaatggtgtc
    66121 cagcatgaag ttacaccttt catcaatagt ctttaccaca gcaacagcac gttcagtttt
    66181 gacaactttt tccatcaagt aaaagcagga aaaacaagcg atgcggaaac gctgatggaa
    66241 aattcattgt ttggcttgaa tcaaggctcc ttgttcacac aattaggcgg aaaaaatact
    66301 ttccaagctg cacctgatat tttaagtcaa acacaaggtt acactagtgc ggtattccat
    66361 gggaacgctg gtacattctg gaatcgaaac gagacctata agaatttagg atatgattat
    66421 ttctttgatt cttcttatta tgatgttaat gatgacaatt cgttccaata cgggttgcat
    66481 gataaaccat ttttccaaca atccgtacaa tacttggaac gtctgcaaca accattttat
    66541 tcaaaattca ttgctgtatc taaccattat ccttattcga aattcaccaa tgacgaagct
    66601 ggattcccat tagccaatac aaacgatgaa acgatcaacg gttacttcgc gacagccaat
    66661 tatttagaca ctgccgtaca agaattcttc aactatttga aagaaagcgg cttgtatgat
    66721 aattcagtta tcgtccttta cggtgaccat tatggtgtct cgaattctag aaataaaaat
    66781 ctagctgaac tagttggaaa aacaagttct acttggacaa attatgacaa tgcacaaata
    66841 cagcgtgtcc cttatatgat ccatattcct ggacaaacac aaggcgggat caatcacact
    66901 tatggcggcc aagttgatgc tttgcctaca ctgctccatt tactaggtgt agatacaaaa
    66961 aattatattc agttaggaca agatctattc tcaaaagatc atgaacagct tgtttctttc
    67021 cgtgatggcg attttgttac tccaaaatat acttactacg gtggtaccat ctacagtaat
    67081 gaaactggtg aagccattac tgaacctact gaggaagtac aaaatgaaat tgattcactc
    67141 aaagaaaaag ttgataaaca gttagctgtt tctgatcaaa tcaataacgg tgatcttctt
    67201 cggttctata cgaatagtgg attagaccca attgatacca gtgattatga ctacaagaat
    67261 ggcttagaga aattaaaaga agctgaaaaa tctctaggtg acaaatcaac tagtgtcttt
    67321 agtcaacaca acggacaatc aactgttgat ctttataaaa cacaaacgta tcaagaactt
    67381 cacggaacga ccacaagttc ttccgaatga taaaataaat ttaaaaggag cagcgacaaa
    67441 attcttgtcg ctgctccttt tttcgaataa acagtgttca aaagcttgca tctacggtaa
    67501 tagaataggg cagcttcttc acaacctctt gctttttaca aaatattttc tggttttaat
    67561 tcgtgatttc actaaaggaa tttcatactt tattcatatt cattttctat actttttata
    67621 gaggtgaaaa gtatgaaaat aatccgctat gctactgctc gattaattta ttacaaaaag
    67681 cagacaatac tctattgttt attttcaagc ttttctgctt ttttattgat agcatgcctg
    67741 actctttacc atttgcaatc ggctttagcc aatcaagtcc aagatcggct ttcatttatt
    67801 ggcgcttctg catctacaga gcttcttccg gctttagaat tagctagccg attttatata
    67861 aaaggcatat tcagtttatt ttttctattt gccttccttt ttatcttttt cttttactat
    67921 tcagtcaggc aaaacaagca ggaattgatc aattggcgcc tgactggtct ttctaaaacc
    67981 aaattattcc tttttatttt ctggcagctg ttctttcctc tctttctttg ttgtatcttg
    68041 gttttattgt ggatcgtcag cttccagtca gtttatgaag cgcttcttca aaggatcaat
    68101 tttcttgtct taaagactac tgaactgcca gatatccgtg atatcttgac ttcggtaagc
    68161 ggactgacaa ttccgatgga tcaacagact ttttttacca tcgatttcca aaatgatctt
    68221 tttttcacag atatacttaa gggatttgcc caaactctta ttggactcag tggatgctgt
    68281 ctcattctta gtctattccc tttcactttt tttatccatc atttgcaaag taggaggcct
    68341 ggacgtaatg aaacctgaat atttgattca tgctgaacac ttgaccgtta ttttgtgtcc
    68401 tctcgaaaaa cagcataaaa tattggagca cttgaaaaaa gaggttcttg ttgcaaaact
    68461 ggataatttt gcgatcatcc aaaaaaattg gccgatgttt ccttatctga atcttaaaga
    68521 ccatgtcctg ttggatgtac cggaaaaaga aatcaaagaa gatcgtttgg cctatcaaga
    68581 aaaacttgat atctctcctt ccttgcttaa ttgtgcggca gctgagttga cgtcttttga
    68641 aaaagtaaag ctgcaattac ttcatgcctt gctttccaag cgaaacaata ttgttattga
    68701 agatacattt gatgaactta gtgtattgga gatccaagag ctactccact tgctttctta
    68761 tttagctcat gaagaaaatc aaggtatctt actctttact catgatgcta ctattgccca
    68821 aagtccttat attgatcgtt tagagcccgc gggctaaatc aatcatttta tagtaaaaac
    68881 aagcggtata gaagatcgat tcatgatctt ttataccgct tgttttttac tcttctttga
    68941 tttctacgtc aggcaaacga acagtgaatt ttgttccttt tcctagttcg ctttctactt
    69001 ctaattttcc tttatgcaag cgtacaagtt gctgcacgat cggtaaacct aatcctgatt
    69061 caccatattt tgtatttttt cttgaaggat ctactttata ataacgatcc cagatacttt
    69121 tcatttgttg ttcagacatt ccaattcccg tatcttcaat cgtgatgatc gtttcaagat
    69181 agcctttttc tagagtaatc gtgatttggc cgttttcagt aaactgtatc gcgttttgaa
    69241 taatattgac catgatttgg acaaatcggt cataatcagc gtaaacatca atatcatcaa
    69301 cggctttcaa tgtcaatgta tctcctgctg cttctgcttt agcttctaac tgtgcaacaa
    69361 tatttttcaa tgtttctgtt ccattgaatt ttttaatgac catactgatt tgattcgtac
    69421 ggattttttc ataatccaag ttttcattca ccaaacggat cagacgctct gtttcatttt
    69481 tcatcaattt aatcgcgttt tctcgctgat tttcagggat tgcattgtat tctaaccctt
    69541 ccagaagtcc gttgattgtc gtaagaggtg ttctcatctc gtgagaagca tctgccataa
    69601 attgtcttcg acgattttct tgttcttcaa tttctgcttg agattctttc aatgaattcg
    69661 tcattttatt gaaatcatct gctaactcat caaattcatc tttgtcatgt acggggagct
    69721 gtacatcaaa attaccattc gtcacttctt tcgtcgcatt ccttaaccga ttgatacgct
    69781 taacttggaa tgctgcaaaa gcatagctgg caatgactgc aacgatactg gaaaaaataa
    69841 atcctttgaa caggttcagt gtaattggac gaacgctgtt atcgatattt cgcgcaggtt
    69901 gactaactac aagtgctcca tagaattcat gattcaaatt aaatggcaca agtgcgtaag
    69961 aggtcgcctg tgcttgtcct aaaatatttt tattcgatgt gaatttttct tgacgtccat
    70021 tcctcagatt tttccattga tcagctgtta aggtaaaatt cagtaaagct gtattagtcg
    70081 gataaatgac acgttcgttt ttatcgataa atataaagtt cacattttgt tctgtcagta
    70141 cttgctctgt cagcagcagt gcattttgaa acgcctgatc ttgactaaac tgcgggagcg
    70201 tatcggcata ggtttgcgtt gtcttttcaa ctgattcagc atatccaaac agttgctggt
    70261 aattgttttc ttcaatcgtt tgttttgtca gttgtgtaaa agaaactcca acaatcaata
    70321 agatcaacgc gaccattccg caaaaagcta agagttgctg atacaaataa cgcattaggc
    70381 aacaccagaa tcatcgaatt tgtagccgac tccccaaaca gtttggatga cttgtggtcc
    70441 gaccttttcg attttttgtc gtaatttctt gatatgggcg tccactgtcc gttcgtcacc
    70501 aaagtactga taatcccaga ctaattcaag cagctgctcc cgagagaata cttgacgtgg
    70561 tttttttgct aaagtaaaca atagatcgaa ctctttaggc gtcagtcctt caattggccg
    70621 gttgtccaga tacgcttctc gtgttttcgt attcatcttg aaatgactag tctcgatatc
    70681 aaaatgatca tctgaaactg ctgcttcttc tcttgcttct gccaactcac tacggcgatg
    70741 caaagctttc attcgagcaa taagcgtcag aggactgaaa ggttttgtga cataatcatc
    70801 tgcccccatc tctaagccga tcacctgatc gctttcagaa tctctagctg tcagcatgat
    70861 aatcggcaca gtacttgaaa ctttgcggat ctctcttcct actagcatac cgtccagtgt
    70921 gggcaaattc aggtctagtg taatcatatc ccaatgttgt gggttttcta aaaaggcatc
    70981 gagcccttct tttccatcat acttgaaagt tgcttcccaa ccttcattta agaaaaacat
    71041 ctgcatcatt tcagaaacag attgattatc ttcaatcatt aaaatattca ttatcgttcc
    71101 ctccagtttc caggtcatat ttctgtaatt tcttttctgt cataataaaa ataggcataa
    71161 ttggaaatac tagcaaaagc atttcaatca gaaaataatt cgtatgccca gatgtcttta
    71221 aataatagcc gatcaatgcc atcagtaagc ccataagaaa aaaccagttt ccgcttgtct
    71281 tttgagccag ccgccaatga gcgtctgttt ttgcggcaag aggcgaatgg tacccataaa
    71341 aaggcagatt tccttttgag ggcagaatcc gaaaaagact gccaattatt aagaataaga
    71401 taccaatact gaaaaaaatc attctttccc tcctaaattc tcttcttata gtatactcta
    71461 aattgttgac tgaaccaata tgttcaggaa ttcttcacga aaggtttgtc ttttgttcat
    71521 tctttgttaa gataaactta ttccaataaa aaaggagttt tatttgtgtc aaattatcgt
    71581 gcaatcactt tctttgatct tgatggaaca ttactagatg cgaaatcaaa aatcacaccc
    71641 gaagtggcaa aagctatgaa cgcattaaaa gaaaacaatg ttctccccgt catcgctaca
    71701 ggacgtacag aagcagaaat ccactctatc atggaagatg cgggcattac ttctgcggtc
    71761 accatgaatg gatctttcat tcgagtagac ggcgaagaaa tctattctca aacattttct
    71821 tcagaagaat gtcaaaaaat gtatgatcat gtacagttga acggtcatga actttcttac
    71881 tataacgatc agaaaatctg gtgtaccggt catagccaaa caatgatcaa tgcttacgac
    71941 ttcattcatt cagatgttcc ggaaattgat cctttaggct ttaaagaaaa cacagtaaat
    72001 atgttgctga tcttatctca atcaggtgac gaatactatc acgaacattt tcctgagcta
    72061 actttttatc ggaatggtcc ttactcgatc gatacagtga agaaaggaat ctcaaaaggg
    72121 gcaggtgtca aacgtctgca aaaagaactt cagctggaaa aagttcctac ttttggattt
    72181 ggcgatggtc cgaatgattt tgccttgttg gaagcttgcg acaacaaaat cgctatgggc
    72241 aacgcctatg acggattaaa agaactttct acctttatca caaagaaaaa tacagaaggc
    72301 ggaattgtcc acgcattgaa gtattttgat ctgatttaag attaagatct tttttttgaa
    72361 gctacctctt ttcttaagaa aatcgcttat tttttcttaa gaaaggggca gttattatat
    72421 aaataatggg atatgatccg cttttttatt gaatttatcc caatatatgc tatactaatt
    72481 ttgtatgaaa aatacacgta acccttgtta gctaacgggg acgttacgga ttcgacaggc
    72541 acagtcgagc ttgaattgcg tttcgtaggt tacgtctacg taaaaacgtt acagttaaat
    72601 ataactgcta aaaacgaaaa caactcttac gctttagctg cctaaaaaca gttagcgtag
    72661 atcctctcgg catcgcccat gtgctcgagt aagggtccta actttagtgg gatacgtttc
    72721 aactttccgt ctgtaagttg aaaaagagaa catcagacta gcgatacaga atgcctgtca
    72781 ctcggcaagc tgtaaagcga atccttaaat gagttgacta tgaacgtaga tttttaagtg
    72841 gcgatgtgtt tggacgcggg ttcgactccc gccgtctcca ttgtgagatt tcaccagatt
    72901 tcgctagatg cttaaatcct tattttataa gggtttgagc attttttatt ttcgcagatt
    72961 ttcgttaaat gaatgaaaat ggcattcaaa atagcattca aaaaaacatt ttttaatata
    73021 tatgttccat ccatattgtc aacgctattc ctactttaca aaaagaacat tagttcgtat
    73081 gctcttctcg aggtgaactt ttatgctgat ggaaggaaaa acacaattat ggtttaaatt
    73141 cgatcctacg aatagattta tcaaagattt ttacaaagta tggaattcag aagttttctt
    73201 tttagcaatc gaagacaatt tattaatcaa tctctactat tctaataaaa actactttaa
    73261 gatccctgct gcgaaaacga gaatgaagaa ggatgtatac tttttgtttg atgtcgtgac
    73321 tgacgtgccg gacgcacgga gcgatcatcg gcgttatgat tatataaagt atactttcgt
    73381 tgatccagaa agatataaag attaagtatg taccgcccct catcgaggag ctatttttat
    73441 ttatagcgtc aaatagatat cagaaacccg cacatcattc accgcacctt catcattgca
    73501 gtgattcatt atatccttaa aactttttta tgatgaaata atattggaat atattcaaat
    73561 ttatagtatt ttagatatag aaaatctatg cgccaaattt attcatagaa aatattgggg
    73621 tgaactttgt tggtaaaaga aaatttagag ttagaagata ttcatcaaaa gagtaaagtt
    73681 attgcaaatg aagttatggt tactgcatct aaagcatctg tacctttaac ttctaatgaa
    73741 aaagcagata tagaacaagt attttcagaa aaaactattg aattaagtga acgagctgat
    73801 cgcattctaa aagaccagcc ttctcttagt gaaaacgaat tagctataaa actgattaaa
    73861 gaagatttaa aaaatgcctc tatgttttct cctatgaaac gcatattaaa aaaagcaatt
    73921 aaacatttgg aggaaaatta atgatttcca atttaaaaca tatcttacct ttactaaaaa
    73981 aaattattat ctttatctat ggacatcgtg aagaactaga gaaatatgca agttatactg
    74041 ctgaggtata cgtcgccgtc gcaagagctg ccaaacaacg acaaaaactc aaacaaaaat
    74101 agtaccgtcc ctcaatgagg ggctattttt tatcgattag gaatcgttaa cacgtattct
    74161 ggaaaacgaa tatcgactgt ttgaccattg atcgtatcgc cgttcggatc gttggttctt
    74221 cgcaacattg ggaatacttt tcttccagca aacctccgaa tgtccaatgt tccatctaat
    74281 ccaaatcgaa gtccactagg taatccgtaa gctgcattca cgtcatcgcg aacaatccct
    74341 gccgatctgc aacgccctac ttcgacattt ggattatctg catccatcca gaaaacgaat
    74401 ccgtacccta agctgggcgc tccgttctgt ggaacgagcc atgcgccaat ccgaaaaaca
    74461 cctaatttta attcattgaa tttttccaat ttaccaacgt aatttccgac ggttttcggc
    74521 gcacttgctg caatgatctc atcatgtgtt tgagctgtgt cagctagacg tcttcgaaca
    74581 ttgctcatat tcacccccgg gcattgatta gatccatgac cgctaaattc gttatgacct
    74641 aggacgtttg ccgaagttaa tccaaaacgg cgcattgcat ctcttgctct cacatcaaaa
    74701 gctcgttcct gcgcttcggt gaaagatccg ttccctacta aacaaatgtg gtaagttact
    74761 gtgttgtgat tacctacacc gttcgtgaca tcgttatctc tataacacaa ctgaactgtt
    74821 ccatctcgta aaataatctc atggtaccca cctgtacccc accctagcga accccgccag
    74881 tgattttgaa atgcccagac atcgccgcta attgtagctg aatgatggcg agcgata
//
LOCUS       DACCSK010000002        70475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCSK010000002 DACCSK010000000
VERSION     DACCSK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.002"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            143..1225
                     /locus_tag="IVP39_000076"
     CDS             143..1225
                     /locus_tag="IVP39_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7585185.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1225..2028
                     /locus_tag="IVP39_000077"
     CDS             1225..2028
                     /locus_tag="IVP39_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7585186.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2098..2838
                     /locus_tag="IVP39_000078"
     CDS             2098..2838
                     /locus_tag="IVP39_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7585187.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2849..3853
                     /locus_tag="IVP39_000079"
     CDS             2849..3853
                     /locus_tag="IVP39_000079"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7585188.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3898..5742)
                     /locus_tag="IVP39_000080"
     CDS             complement(3898..5742)
                     /locus_tag="IVP39_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585189.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            5954..6472
                     /locus_tag="IVP39_000081"
     CDS             5954..6472
                     /locus_tag="IVP39_000081"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7585190.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            6600..7283
                     /locus_tag="IVP39_000082"
     CDS             6600..7283
                     /locus_tag="IVP39_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7585191.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            7592..7665
                     /locus_tag="IVP39_000083"
     tRNA            7592..7665
                     /locus_tag="IVP39_000083"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7626..7628,aa:Arg,seq:tct)
     gene            7824..8183
                     /locus_tag="IVP39_000084"
     CDS             7824..8183
                     /locus_tag="IVP39_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7585192.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            8306..9937
                     /locus_tag="IVP39_000085"
     CDS             8306..9937
                     /locus_tag="IVP39_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7585193.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(9999..10640)
                     /locus_tag="IVP39_000086"
     CDS             complement(9999..10640)
                     /locus_tag="IVP39_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7585194.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            10922..11230
                     /gene="rplU"
                     /locus_tag="IVP39_000087"
     CDS             10922..11230
                     /gene="rplU"
                     /locus_tag="IVP39_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7585195.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            11257..11601
                     /locus_tag="IVP39_000088"
     CDS             11257..11601
                     /locus_tag="IVP39_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7585196.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            11614..11907
                     /gene="rpmA"
                     /locus_tag="IVP39_000089"
     CDS             11614..11907
                     /gene="rpmA"
                     /locus_tag="IVP39_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7585197.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            12314..15148
                     /locus_tag="IVP39_000090"
     CDS             12314..15148
                     /locus_tag="IVP39_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7585198.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            15339..15830
                     /locus_tag="IVP39_000091"
     CDS             15339..15830
                     /locus_tag="IVP39_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7585199.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            15933..16922
                     /gene="manX"
                     /locus_tag="IVP39_000092"
     CDS             15933..16922
                     /gene="manX"
                     /locus_tag="IVP39_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7585200.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            16955..17758
                     /locus_tag="IVP39_000093"
     CDS             16955..17758
                     /locus_tag="IVP39_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7585201.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            17779..18693
                     /locus_tag="IVP39_000094"
     CDS             17779..18693
                     /locus_tag="IVP39_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7585202.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            18776..19150
                     /locus_tag="IVP39_000095"
     CDS             18776..19150
                     /locus_tag="IVP39_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7585203.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            19289..19534
                     /locus_tag="IVP39_000096"
     CDS             19289..19534
                     /locus_tag="IVP39_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7585204.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            19706..20770
                     /locus_tag="IVP39_000097"
     CDS             19706..20770
                     /locus_tag="IVP39_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7585205.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            21016..21441
                     /locus_tag="IVP39_000098"
     CDS             21016..21441
                     /locus_tag="IVP39_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7585206.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            21434..21883
                     /gene="nusB"
                     /locus_tag="IVP39_000099"
     CDS             21434..21883
                     /gene="nusB"
                     /locus_tag="IVP39_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7585207.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            21980..22834
                     /locus_tag="IVP39_000100"
     CDS             21980..22834
                     /locus_tag="IVP39_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7585208.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            22835..24172
                     /locus_tag="IVP39_000101"
     CDS             22835..24172
                     /locus_tag="IVP39_000101"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7585209.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            24177..24401
                     /locus_tag="IVP39_000102"
     CDS             24177..24401
                     /locus_tag="IVP39_000102"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7585210.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            24403..25293
                     /locus_tag="IVP39_000103"
     CDS             24403..25293
                     /locus_tag="IVP39_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7585211.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            25302..26120
                     /locus_tag="IVP39_000104"
     CDS             25302..26120
                     /locus_tag="IVP39_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7585212.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            26227..26676
                     /locus_tag="IVP39_000105"
     CDS             26227..26676
                     /locus_tag="IVP39_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7585213.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            26694..28376
                     /gene="recN"
                     /locus_tag="IVP39_000106"
     CDS             26694..28376
                     /gene="recN"
                     /locus_tag="IVP39_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7585214.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(28431..28547)
                     /locus_tag="IVP39_000107"
     CDS             complement(28431..28547)
                     /locus_tag="IVP39_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7585215.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(28620..29576)
                     /locus_tag="IVP39_000108"
     CDS             complement(28620..29576)
                     /locus_tag="IVP39_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7585216.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            29747..30115
                     /locus_tag="IVP39_000109"
     CDS             29747..30115
                     /locus_tag="IVP39_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7585217.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            30285..30716
                     /gene="mraZ"
                     /locus_tag="IVP39_000110"
     CDS             30285..30716
                     /gene="mraZ"
                     /locus_tag="IVP39_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7585218.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            30737..31696
                     /gene="rsmH"
                     /locus_tag="IVP39_000111"
     CDS             30737..31696
                     /gene="rsmH"
                     /locus_tag="IVP39_000111"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7585219.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            31701..32102
                     /gene="ftsL"
                     /locus_tag="IVP39_000112"
     CDS             31701..32102
                     /gene="ftsL"
                     /locus_tag="IVP39_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7585220.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            32103..34295
                     /locus_tag="IVP39_000113"
     CDS             32103..34295
                     /locus_tag="IVP39_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7585221.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYPYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            34321..35283
                     /locus_tag="IVP39_000114"
     CDS             34321..35283
                     /locus_tag="IVP39_000114"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7585222.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            35297..36658
                     /locus_tag="IVP39_000115"
     CDS             35297..36658
                     /locus_tag="IVP39_000115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7585223.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            36684..37772
                     /gene="murG"
                     /locus_tag="IVP39_000116"
     CDS             36684..37772
                     /gene="murG"
                     /locus_tag="IVP39_000116"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7585224.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            37787..39007
                     /locus_tag="IVP39_000117"
     CDS             37787..39007
                     /locus_tag="IVP39_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7585225.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            39163..40488
                     /gene="ftsA"
                     /locus_tag="IVP39_000118"
     CDS             39163..40488
                     /gene="ftsA"
                     /locus_tag="IVP39_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7585226.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            40511..41752
                     /gene="ftsZ"
                     /locus_tag="IVP39_000119"
     CDS             40511..41752
                     /gene="ftsZ"
                     /locus_tag="IVP39_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7585227.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            41769..42446
                     /locus_tag="IVP39_000120"
     CDS             41769..42446
                     /locus_tag="IVP39_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7585228.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            42460..43059
                     /locus_tag="IVP39_000121"
     CDS             42460..43059
                     /locus_tag="IVP39_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7585229.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            43074..43361
                     /locus_tag="IVP39_000122"
     CDS             43074..43361
                     /locus_tag="IVP39_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7585230.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            43410..44192
                     /locus_tag="IVP39_000123"
     CDS             43410..44192
                     /locus_tag="IVP39_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7585231.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            44213..44920
                     /locus_tag="IVP39_000124"
     CDS             44213..44920
                     /locus_tag="IVP39_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7585232.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            45182..47980
                     /gene="ileS"
                     /locus_tag="IVP39_000125"
     CDS             45182..47980
                     /gene="ileS"
                     /locus_tag="IVP39_000125"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7585233.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(48029..49555)
                     /locus_tag="IVP39_000126"
     CDS             complement(48029..49555)
                     /locus_tag="IVP39_000126"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7585234.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(49570..50217)
                     /locus_tag="IVP39_000127"
     CDS             complement(49570..50217)
                     /locus_tag="IVP39_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7585235.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(50401..50730)
                     /locus_tag="IVP39_000128"
     CDS             complement(50401..50730)
                     /locus_tag="IVP39_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7585236.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            50907..51635
                     /gene="sfsA"
                     /locus_tag="IVP39_000129"
     CDS             50907..51635
                     /gene="sfsA"
                     /locus_tag="IVP39_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7585237.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            51651..52664
                     /locus_tag="IVP39_000130"
     CDS             51651..52664
                     /locus_tag="IVP39_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7585238.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            52664..53941
                     /locus_tag="IVP39_000131"
     CDS             52664..53941
                     /locus_tag="IVP39_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7585239.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            54004..56706
                     /locus_tag="IVP39_000132"
     CDS             54004..56706
                     /locus_tag="IVP39_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7585240.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            56858..57175
                     /locus_tag="IVP39_000133"
     CDS             56858..57175
                     /locus_tag="IVP39_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7585241.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            57205..57525
                     /locus_tag="IVP39_000134"
     CDS             57205..57525
                     /locus_tag="IVP39_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7585242.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            57633..59093
                     /locus_tag="IVP39_000135"
     CDS             57633..59093
                     /locus_tag="IVP39_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7585243.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            59161..59382
                     /locus_tag="IVP39_000136"
     CDS             59161..59382
                     /locus_tag="IVP39_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585244.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            59413..59595
                     /locus_tag="IVP39_000137"
     CDS             59413..59595
                     /locus_tag="IVP39_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7585245.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            59595..60008
                     /locus_tag="IVP39_000138"
     CDS             59595..60008
                     /locus_tag="IVP39_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7585246.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            60131..61312
                     /locus_tag="IVP39_000139"
     CDS             60131..61312
                     /locus_tag="IVP39_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7585247.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            61701..61774
                     /locus_tag="IVP39_000140"
     tRNA            61701..61774
                     /locus_tag="IVP39_000140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61735..61737,aa:Arg,seq:cct)
     gene            complement(61843..62982)
                     /locus_tag="IVP39_000141"
     CDS             complement(61843..62982)
                     /locus_tag="IVP39_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7585248.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(63281..63916)
                     /locus_tag="IVP39_000142"
     CDS             complement(63281..63916)
                     /locus_tag="IVP39_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585249.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(64029..64664)
                     /locus_tag="IVP39_000143"
     CDS             complement(64029..64664)
                     /locus_tag="IVP39_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP7585250.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(64698..65159)
                     /locus_tag="IVP39_000144"
     CDS             complement(64698..65159)
                     /locus_tag="IVP39_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP7585251.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(65289..65720)
                     /locus_tag="IVP39_000145"
     CDS             complement(65289..65720)
                     /locus_tag="IVP39_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7585252.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(65738..66058)
                     /locus_tag="IVP39_000146"
     CDS             complement(65738..66058)
                     /locus_tag="IVP39_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7585253.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            66357..67133
                     /locus_tag="IVP39_000147"
     CDS             66357..67133
                     /locus_tag="IVP39_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7585254.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            67148..67351
                     /locus_tag="IVP39_000148"
     CDS             67148..67351
                     /locus_tag="IVP39_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7585255.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            67367..67705
                     /locus_tag="IVP39_000149"
     CDS             67367..67705
                     /locus_tag="IVP39_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7585256.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            67692..67871
                     /locus_tag="IVP39_000150"
     CDS             67692..67871
                     /locus_tag="IVP39_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585257.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(67914..68384)
                     /locus_tag="IVP39_000151"
     CDS             complement(67914..68384)
                     /locus_tag="IVP39_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585258.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            68471..69169
                     /locus_tag="IVP39_000152"
     CDS             68471..69169
                     /locus_tag="IVP39_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7585259.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            69347..69688
                     /locus_tag="IVP39_000153"
     CDS             69347..69688
                     /locus_tag="IVP39_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585260.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            69681..70352
                     /locus_tag="IVP39_000154"
     CDS             69681..70352
                     /locus_tag="IVP39_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7585261.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
BASE COUNT        23317 a        11766 c        14722 g        20670 t
ORIGIN      
        1 aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt ctgaataaaa
       61 gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat cacaatatga
      121 atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta ttgatttaac
      181 ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca gagaatatgc
      241 caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg ccaaacatgg
      301 tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg gttttatggt
      361 aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt ggtgggggca
      421 agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt
      481 gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac cttatgaaat
      541 aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag agtggggcat
      601 acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg gcagtaaata
      661 tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc gagtattaga
      721 gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg tgcaagaaga
      781 agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata ttgcttttgc
      841 tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag cagattcgat
      901 tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc ataaaaaatt
      961 attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat atacagtgat
     1021 ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg ttccgtctgt
     1081 agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc atgaagatga
     1141 ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata gtgaaacagt
     1201 tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga atatcggaaa
     1261 aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt tttcttatct
     1321 ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt tccggaagaa
     1381 agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt cgcattcaac
     1441 tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa aagtaacctt
     1501 ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa tttgatcaat
     1561 tcagtcatca actggctact caacagttta atctttctgc ccatgttttt aatcagcgga
     1621 gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca
     1681 gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt tgtttcagca
     1741 ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa gttcgactat
     1801 cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa agatagaatt
     1861 ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg agcaatcgct
     1921 ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc agaattttat
     1981 gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca tgtaagcagt
     2041 ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga atgtacgatg
     2101 aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg gacaacaggt
     2161 tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc tacgctgatc
     2221 aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag ccattatcat
     2281 catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca tcctaagaga
     2341 aatccggaga cattgctttc aatttatgga cacacaaaag attccattca ctttgaagaa
     2401 ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga attaaaaatt
     2461 ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta cgccatgcgt
     2521 atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg ttatttggaa
     2581 agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta tctttttgaa
     2641 ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact tgcgaaatca
     2701 gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt agaacaattg
     2761 aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc tgcagttgac
     2821 aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa accacgaccc
     2881 aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag atgaacctat
     2941 tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa atacgcttga
     3001 agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg
     3061 aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca tgcctgatga
     3121 tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag cacttcatga
     3181 attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg atggcatgaa
     3241 attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg gaacaattat
     3301 gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga
     3361 atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat ttcttccaga
     3421 agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga aaattttcgg
     3481 cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt ggaaaaaaat
     3541 caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga agtctccaga
     3601 tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc atttgaatgt
     3661 ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac taggagatat
     3721 ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc tatcaaaaat
     3781 agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag aattgcttac
     3841 cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg ctatttttta
     3901 tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga aagtatcttt
     3961 ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa
     4021 acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg tatcgtaatg
     4081 tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat
     4141 ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct ctaaagcgtg
     4201 gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg
     4261 gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta agataagatt
     4321 tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt cttctaatat
     4381 cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt gcagattctc
     4441 catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct gtcgaagctg
     4501 ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt gtctccgatc
     4561 tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga ttagaatgat
     4621 aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg ccgtttggat
     4681 gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag ctacataaaa
     4741 acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag tgcttataac
     4801 actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa aagttgcata
     4861 aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt accatttttt
     4921 atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta agataataaa
     4981 taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta aaatatcttc
     5041 tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa gtgagtattc
     5101 aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta tagcgataaa
     5161 aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa aactaatcaa
     5221 tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct gataaggaga
     5281 tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta tcacagccaa
     5341 tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa tcttttcgcc
     5401 tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt cgtccaacgg
     5461 tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag gaatcgtacg
     5521 agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa cccacgaatc
     5581 ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa agaaataagc
     5641 aattcctgtt aaaaataaca atgataatat aatgattatg ctccacttta tacgtaaata
     5701 aaaagattct tttttattat atatgttatt aaaaaaagac ataaaggtat tcctttctaa
     5761 aacttgtaat gttagggtaa acgtttttta aactaacgtc aattatttat aataaaaaat
     5821 gagataaaga tgaggaaata gaattagaaa catataatta ttaaagggaa acatttttac
     5881 agctataatt tgaaaaaaat ttatcgttat tatatgatta agaaggattg aataaagaaa
     5941 gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat
     6001 tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc
     6061 ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt
     6121 acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc
     6181 aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta
     6241 tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat
     6301 cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc
     6361 atggattcag gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa
     6421 tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta
     6481 aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca
     6541 ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta
     6601 tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta
     6661 cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag
     6721 aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc
     6781 agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca
     6841 cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga
     6901 aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc
     6961 aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg
     7021 caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg
     7081 ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc
     7141 cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc
     7201 atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg
     7261 aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat
     7321 cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata
     7381 caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat
     7441 aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc
     7501 aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt
     7561 atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc
     7621 cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag
     7681 aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag
     7741 gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac
     7801 tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg actctagttg
     7861 caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa
     7921 gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta
     7981 agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt
     8041 cttccgcatc taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat
     8101 atggcagttt gactagcgat cgttggattt tactcaaaca aggtgggcta gctctgttgg
     8161 cattgctttc tattttctta taaatataga aaaaataaga atttatctaa caggaaactt
     8221 tgtgaaaaat attactttat ctcatgaaat atgatattct aatgaatggt attttagaaa
     8281 atttcatgaa aaaggagtta gataggtgtt caaaaattat gtttcctcac taaaaaaaga
     8341 attttcaggt tatgatcttt caagatttca gaaagatctt ttggctggga tcacagtagc
     8401 tgctgtagca ttgcctttag ccttggcttt tggggtatca agcggagctg atgcggccgc
     8461 aggtttgatt acggctatca ttgccggtat cgttattggc ggtttatcag gcggttttta
     8521 tcaaatatca ggtccgacag gtgcgatggc agctatcttg atgtctattg cggcaacaaa
     8581 gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt ctatttttac ttgcagcagg
     8641 acttttccga ttaggaacac tgacttcttt tatcccagca cccgtaatta cggggtttac
     8701 ttcaggtatt gctatcatta ttgcattagg tcagatcgat aatttatttg gtgttcactc
     8761 ggaaggtaca aacgttattg aaaaactaag tagttatcaa acgataggat ttccgataaa
     8821 cacagcctct ttgatcatgg gagggctagt cattttaggg atgtttatct atccaaaaaa
     8881 atgggcgaaa cgaatgccta gttcgctgct ggctatcatt ttggcaacag cagttatgct
     8941 attgttccat ttaccagtag caactgtggg taagattcca caaacattga tcagtagtaa
     9001 tcgtttgaat atgggagatt ttagcttttc tgccttgcaa caagtggcag ttccagctat
     9061 tagcattgct ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg ctggccgaat
     9121 ggcaaataag cccttagata gcaatcaaga attaatggcg caaggaattg gaaatctgtt
     9181 gcttcctttc tttggcggga ttcctgctac tgcggccatc gcccgtacaa gcgtggccat
     9241 caaatctggt gcacaaacta gattagctgg tatgatacat gcagttgtct tactgctctc
     9301 catgcttgtt tttgcgccag tgatgtcaca gattccaatg ccagcacttg caggtgtttt
     9361 gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc aaggagctat ttactaataa
     9421 atattggtca gcagttctat tgtttatcct tacgatgatt tgtacagtta tctttgattt
     9481 aagtattgct atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg tcaaaagcgc
     9541 tgctatcacg atttcattag aaacgatcga ttggcaaaga atggggctgc cagaaacgga
     9601 aaagctgaat aactggacag tagtgtatat tagtggtcca ctgtttttta tgtcttcaga
     9661 aaaactcaaa aataaattgg aagaactaaa tcatcacgat ggtgtcattt tctccatgcg
     9721 aggcgtacca agtattgatt tgacggcacg tgcagttttt gatgaatttc aagaaaaagc
     9781 agagcaaaga ggacaaacga ttatctatac ttccttgcag ccagaagtag aaaaacaact
     9841 ttctcacttg tgggaacata cattcacgca tcaacatcaa acagttgctc atgcgttgac
     9901 agctttacat gcgaaatata ctgcaattag ccattaaaac aatgaaaaag aacagacaag
     9961 actgtaaact gaagatttta tggtcttgtc tgttcttttt aatattgctg taaaagtgaa
    10021 gctagtactt gccagtcttg atggataaca ggtacaaggg agcgattgta gaaaatagct
    10081 gctggatgaa aaagtggaag aattcgatag tttttttctg accattcgta ggcatccttt
    10141 tgttctgtta attgtaagac aggccctgta tagagttgac catgattttt ggtaaccgta
    10201 tattctttgc caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat taattttggc
    10261 tggaccttca tcagttcgta atccaatatt ggtgcatgcg caaatacttc ggaacgagta
    10321 ggtgtccgat ttggataaac agtttctgac tgttgtgtcc gtttattgat ccgatgagtc
    10381 acgcgatacg ggcggctcct tacagcactg gtgatataca cctcttctcg tgttaagcca
    10441 gttgaagaaa gtgcctgcat caattctttt ccagcttgac cgctaaaagg aacatgcgta
    10501 tcaatctctg ttttccctgg ggcttctccc acgagcatca gtttaggatg aagaggtcct
    10561 tgcccagcga taaatccttc tacaggacgg ccgttcattc ttaaagaagc ttcttgtatc
    10621 agttcttttg ggtattccat ttatgctcct cctttttctt ttattataag aataaaagaa
    10681 acacaatgcg actattttat ttggtagaac tgtgaggata gaaaagcaga agtattgctt
    10741 gaatttttgt tttacttttt gattttaaat ttagttgact aacggttgct gttctggtaa
    10801 gctagtactg ttgtaattgt ggactccaca gctacaaccg ctcagaacaa gtaataagca
    10861 cagcatatgc tgtcacttcg gatggcgagt ctaagtatat cagaaggagg tgctttatag
    10921 catgtacgca attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg gacaagcaat
    10981 ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg aagttatctt
    11041 agtaggcgga gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa ctgttgaagg
    11101 tactgttgaa aaacacggca aacaaaagaa agtcgttact ttcaaataca aacctaaaaa
    11161 acattcacac cgtaaacaag gtcatcgtca accatataca aaagtggtta tcgacgcgat
    11221 caacgcttaa tagcgtttga gaaagaaggc ctatatatga tcaaaggaac atttaagcga
    11281 aatgacgcag gtcagatcgt ttcattcact ttgaccgggc atgctgatgc tggtccttat
    11341 ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt caacgggatt
    11401 gcctcattag ccggttttga gccgattgtt gaaatgaatg aagaagaagg cggttatctt
    11461 tataccgaag tcacttcagg aatgacacaa gaacaaaaca atattgctga gattctatta
    11521 gaaaatcttt tattaggatt acaatcgata gaagctgaaa actcagaata tatccaaatt
    11581 aaaacaatta atgataaata ggaggtgcag accatgttgt tatctatgaa tctacaatta
    11641 tttgcccaca aaaaaggtgg cggttctaca tctaacggcc gtgactcaga atcaaaacgt
    11701 ctaggtgcta aacgtgctga tggacaaaca gttacaggtg gatcaattct ttaccgtcaa
    11761 cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg gtggagacga cactttattt
    11821 gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa acaagtgtct
    11881 gtttatccag tagctcaaga agcttaattg aatcttatga ttaaagaggt tgtgacaaga
    11941 cttttgtcac agcctttttt gtttataaaa ataaaagttg tagtggaggc tacgaagttt
    12001 actttgtctt tttacactta atattttttt gccaatttgg aatcaggatg ttttgataac
    12061 tatcgttttc aattgatgga agctatattg tttgctatca gtgaaataat tatatggtaa
    12121 acagtgttct ttttcttgtt tccattataa aaatcgtatc gggatttttc gttcttttaa
    12181 attgaacatt tctatctatc cgcgtgttat caacaaaaca aaatcaggta aatagttgac
    12241 gaaacagaaa taaatacata gtattatgtt ttgaagccta agtacaatag tctatagaaa
    12301 aggagcgata ccattggcaa aacgcattga ccgaatttat atgtatgtca aagaaaaaac
    12361 cgctcattta acaaaggcag aatatgatca aggagtaaca acacaggaaa ttgctgaagt
    12421 gttagggatt cagcgaacaa atagcagtaa agatctgaat cagctagtgc gcgaaggaaa
    12481 actgataaag acagacggac gaccagtacg ttacatatat caaacactcg ttacgcacgt
    12541 aaagccagtt acaaaacatg tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa
    12601 tacactccaa atagatacta aggatatctt tgcaaaaatc attggagcta acggcagcat
    12661 gaaaaattca gtggaacagg caaaagctgc gattttgtat ccgccaagag gattaaattg
    12721 tttgattaca ggacctaccg gatcaggaaa gacttatttt gcacacgcga tgtatcattt
    12781 tgcaaaagcg catcatgtaa tagatgagga aacggaactg attgttttca attgtgctga
    12841 ttatgccaat aaccctgagc ttttgatgag tcatttattt gggtacgcaa aaggtgcttt
    12901 tacaggcgca gatgaagaaa aaatggggat tatcgatcaa gcggatggcg gtatgctgtt
    12961 tttggatgag attcaccggt tgccgcctga aggtcaagaa atgatctttt actttatgga
    13021 tcatggaact tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa atgtacggat
    13081 cgtaggtgcg accacagaag acccaggttc ttctctatta gaaacttttg ttcggcgtat
    13141 cccaatcaat atcaaacttc cggcttttga aaaacgtcct gcaaatgaga aagtcgattt
    13201 agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga
    13261 agatgtagca aaagcattga tcggcagtgt gacctatgga aatataggac aattgaagtc
    13321 aaatatccag ttagtatgtg cacgaggatt tctaaatcat atgcaatcac ccgaaatctc
    13381 gatcacaatc gatgatttga cagaagggat tcgtagcgga ttgattcaat tagcaagcaa
    13441 ccgcagtgca atggcagagc tttctaaaat attggagcca aagataactg tagcaccaaa
    13501 tgatacgatc atgaaaatcc aatcggactc ctatgagtta ccttataatc tatatgatat
    13561 catcggagat aaagcagctt tactgaagtc ggatggtttg gatcaagaag caatcaatca
    13621 tttcatttct acagatatca acatccatct gaagtctttc tataaagacc atggtttttc
    13681 ttttaatgca gacagcaaat tagcagaatt tgtagatccg aaaatcatag aagtgaccaa
    13741 tcagatttat gtaatggtca aaagatcttt accatatgag tttcagcaaa actttattta
    13801 tgctatgagc ttgcatatca gttctttttt gaaaaaaatc aacttaggag aagaacggca
    13861 tacgaatgat aacatccgtg agatggcttt tgattttcca gatgaatatg ctgttgcgaa
    13921 agaagtgcgt agttatatcg aaacgtattt tcaagtggag attccagaaa gtgaagacta
    13981 ttacttgacc gttctactcg tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt
    14041 agtcgttgca gctcacggaa acagcacagc aagcagtatg gtccaagttg tccaacagtt
    14101 gttagatgca gagaacgtac gagcagtaga catgcctttg gatatggatc cgaaaacagc
    14161 attggaccga attgaacgca atgtacaaga agtagatgaa ggaagcgggg tcatactctt
    14221 agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc
    14281 agtacgtact gttgatatgg taacaacttc tctagttttg gaaactgtac gaaaggtctc
    14341 agtattaggg acagatctag atatgttgca tgaatcattg aaaaattttc gaggttacgc
    14401 agaaatatct tctgtagaat ctgttcagga agcaaaacct attcataaga aaaaagctgt
    14461 tttggctatc tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg
    14521 agcggtaaac aagcgtcaag aaacagaatt agaagttcta gctttgtcga ttgtggaagt
    14581 gaaagaagaa ctgccagtta tccaaaaaga atatcagatc atagctacta ctgggattac
    14641 tgatttgaat atccaagccc catttattcc tttagaacgt tttattgatc aaaatatcga
    14701 agtcattttg gatcagttgc tgatggaatc agaattggag gaaactgaat tcatctccct
    14761 agatgaagag tcagctaaaa atacgtgtgt tgagtttatc tctgataact tcatctttat
    14821 taatggtagt aagttaatcg atccaatgtg gcaattttct acacaaatct ctcagacgac
    14881 gggaatcggc gatgaagaat atggattcaa aatcaaccta gtgatgcaca cagcaggaat
    14941 gatcgaacgt attattcgga atgaaccatt gactgtggaa gaaaacgaat tgacaaatac
    15001 aacgaatgat cctctttata gccaattggc agcctcagtt gttctgttgg aagatcagat
    15061 aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta cgtctagtcc ataatcagtt
    15121 agataaaaaa gaatacactg tgccatgaat acacaaaaat gatacacttt tatagtgtat
    15181 cgtttttgtg tattggttaa gaatccttta aattcaatta gtgtatcgta aaaaagttgg
    15241 cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg
    15301 ggtttctgtg aagtagtgag gaagaaggag ttttgcagat ggatattcga ttggcacgta
    15361 tagatgatcg tttaatccac gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg
    15421 aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa attgagaaag tttctactaa
    15481 aagaagctgc accgccgggg atcacagcaa atgtaataac cgtgcagaaa atgattcaaa
    15541 tctatcatag tggattatta tcacgagcaa aagtgatgct tttattcacc aatccccaag
    15601 atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc ggcggtatga
    15661 gttttactga aggcaaatca atgattacca attttgtttc agtaaatcaa caagatattg
    15721 attcctttcg ttacttgaac agtcaagaag tggagttaga gattcgaaaa gtacctgctg
    15781 acaggaaaat ccttttgatg gaactactag cgaaaatcaa aaaggtgtag caagaatggt
    15841 tgttggaaaa ggtcaaataa ttctgttgta atggttagca attattaata tttttgaatt
    15901 tgttacaaca aaagtaaata ggaggtataa atatggtagg gattatccta gcaagtcatg
    15961 gacaatttgc tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa caagaaaacg
    16021 tcaaagccgt gattttgaaa ccaagtgaag gtccagatga cttgagagcc aaattggaag
    16081 aagcggttgc ttcttttgat aatcaagacg aagtgttatt cttagttgat ttatggggcg
    16141 gaacaccgtt caatcaatca aacacattat tcgaagagca caaagataaa tgggcaatcg
    16201 tcagcggttt gaatttacca atgttgattg aagcctacgc atcacgcttt tcaatggaat
    16261 ctgctcatga aattgcagca cacatcattg aaacagcaaa agacggtgta aaagtaaaac
    16321 cggaagaatt agaaccggca gaagcaccga aagcagcagt tgaagacgca caacctaaag
    16381 gagcacttcc agaaggaact gtggtcggag acggaaaaat caaatatgta ttagcacgtg
    16441 ttgactcacg tctgttacat ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa
    16501 accgtattat cgttgtttca gatgcagtat caaaggatga tctgcgtaag agactaatcg
    16561 aacaagctgc tcctccaggt gtaaaagcaa atgttattcc tattagcaaa atgatcgaag
    16621 tagcaaaaga ccctcgtttt ggaaatacca aagcattgct tttatttgaa aatcctgaag
    16681 acgtattgac agctgttgaa ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg
    16741 cgcattcggt cggtaaagtc gttgtaagta aagtattgtc tatgggacaa gaagacgttg
    16801 aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg
    16861 attcacgcga taacatggat gatattttga aaaaagcaaa agctgaatta gcaaaagcgt
    16921 aataaataat attaaaattt ataggaggct tatcatgtct attatttcaa taattttagt
    16981 cgtattagtt gcctttctag ctggtatgga aggtattctt gatgaatttc aatttcatca
    17041 accactagtg gcatgtacat tgatcggttt agttaccggt aacctcgaag ctggtatcgt
    17101 acttggcggt acgcttcaga tgatcgcttt aggttgggca aatatcggtg ccgctgtagc
    17161 accagatgct gcattagcat cagtagcatc agcaattatt ttagttttag gtggacaagg
    17221 tgttaaaggt gttccttctg cgatcgcgat tgccgttcct ttggctgttg cagggttgtt
    17281 cttaacaatg atcgtacgta caatcgctgt tccaatcgtt catttgatgg atgcggctgc
    17341 tgaagaagga aacatccgta aggttgaaat gtggcacatc attgctgtat gtttacaagg
    17401 ggttcgtatc gcggtcccag cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc
    17461 attccttgaa tcaatgccag catggttaac agatggtatg gctatcggtg ggggaatggt
    17521 cgtagctgta ggttacgctt tggtaatcaa catgatggct acaaaagaag tatggccatt
    17581 cttcgttatt ggtttcgttg tagctgcaat ttctcaatta acattgattg ctctaggtgc
    17641 tttaggtgtt gcattagctc ttatttactt gaacctttct aaaatgggtg gttcatcaaa
    17701 taatggaggc ggaagcaact ctggtgaccc tttaggcgac attttgaatg actattaatc
    17761 ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta
    17821 gctgttgcgt ggcgctctac cttcattcaa ggttcatgga actacgaacg tatgcaaaac
    17881 ggtggctggg cattttctat gattccagca atcaaaaaat tatacaaaac aaaagaagaa
    17941 cgttctgcag cattaaaacg tcacttggaa ttctttaata cacacccata cattgcttct
    18001 ccaattttag gggttacatt ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat
    18061 gatgtagcga ttcaaggggt taaagtaggt atgatgggtc ctttagctgg tgttggtgac
    18121 ccagtgttct ggtttacagt acgtccaatg ctaggtgcac taggtgcttc attagctatg
    18181 ggaggaaata tccttggacc aatcatcttc ttcttaggtt ggaacattat tcgttgggca
    18241 ttcatgtggt atacacaaga atttggctac aaagctggtt ctaaaattac tgatgacctt
    18301 tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta
    18361 gctgcccttg tacaaagatg ggtatcaatc aaattcttac cagttgtatc aactgtaaaa
    18421 ttagataaag gtgcttacat cgaatgggat aaattgcctt caggtggtga aggaatcaag
    18481 agtgcatttg aacaagttaa taatggctta gccctttctc ctgaaaaggt aacaacatta
    18541 caagacaact tagaccaatt gattcctggt ttagctgcat taggattaac attgttatgt
    18601 atgtggttgc ttaagaaaaa ggtttctcca atcgttatca tccttggttt attcgtagtc
    18661 ggtgttgttg gacacgtcat cggactttta taatccataa aaatatagct cgggcgttgt
    18721 cccgagcttt ttttatatag tatactgttc ataaacaaat atctggaggg agaaaatggt
    18781 tcaatcaatc aatacaaaag tagacttggt aattgatgct acttcgttta cgggtctgac
    18841 agattacggg caaatcatga taggtgataa tggttttgag ttttaccatg cgcgagatag
    18901 acgcaaatat atccaaattc catgggaaga agtggattat gtactagcat cagtcatgct
    18961 aaagggaaaa tggattcctc gatatgccat tcaaacgaaa aaaaatggta catacacatt
    19021 ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag atgaagatag
    19081 aatggtacaa tcattgagtt tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa
    19141 acgcaagtaa aaaataaaga aaacttctaa gaggaggttt tctttatttt tttactttgc
    19201 agaagaaaat atgaaatagc aaaaccaatt atttgtcccc tttaaatccc atatgctaaa
    19261 attgattagc aaagaaggag gttatattat gagttggtta tggacattga ttgttggagc
    19321 cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac
    19381 gaatatcatt gctggactag tcggttcaag tattggacaa gcgattttcg gcgaatgggg
    19441 accaaaagct gcaggtatgg cacttattcc atctatttta ggcgcagtga ttttggtagc
    19501 agttgtctca tttttctttg gaaaacgatc ataagttttt acaaaatagt aaactaaaag
    19561 gagttgcagc gagactgtga ctcctttttg gctttattta tttaaaataa aaagaagttt
    19621 tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc
    19681 gtgcaaagaa agagggagtt tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat
    19741 gcaagaagaa aatttggatt catttttggt taccagtccg tacaatcttc gttacttaac
    19801 gaactttaca ggtacgacag gattagctgt tattactttg gaaaaagcat tttttattac
    19861 tgattttcgt tataccgagc aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa
    19921 tgtcggacct atttttgatg aggtagcaaa cttagtacgc aaagaagaac tgcactcatt
    19981 agcttttgag gaaacaacag tctcattttt agaatattcg gttttagaag aaatcatcga
    20041 tgcggaatta gtacctgttt cacagatgat cgaagaactc agagaagtga aagatgaaga
    20101 agaaatcgct attatcgaaa aagcatgtca tattgcggat atggcatatg atcacatcct
    20161 gaaaatgatc caaccgggca tgactgagat tgaagtcgca aatcaactag acttttatat
    20221 gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg gattgcgttc
    20281 ggctatgccg catggtgtag caagcaaaaa aatcatcgaa caaggtgact tgatcacatt
    20341 ggactttggt tgctattacg aagggtacgt ttccgatatg acacgtacat tcgctattgg
    20401 tgatcctggg gaaaaattaa aagaaattta tcaaatagtc ttagatgctc aattagcagt
    20461 tattgatgct gcaaaaccag gaatgactgg tgtccaatta gatgcagttg ctagagatta
    20521 tatttcaaaa catggttacg gggaagcatt tggacattct acaggacatg ggatcggttt
    20581 agagatccac gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa
    20641 tgtgatcact gatgaaccag ggatctactt accaggaatc ggtggagtac gtattgagga
    20701 tgacttgttg atcacagaag atggaaatcg tgtgttgact cgttcaccaa aagaattgat
    20761 catcttatag tttcatcaat agaagaagta agcgtaaaaa tcagtgttca cagactttgt
    20821 tatagaaaat cactcgattt aatgatacac tatagaagaa cttttaagct gtaagagata
    20881 ataaagttgg gtgttttctc caattcatag cagtttgcag ctgatcggcg gcagttttct
    20941 taccacgtga tcactatgcc gcatcataga gaattcggat gtatctcgtg cttaggcaaa
    21001 gggaggaata aataaatggc agatgaaaaa aatttgattt taaacgcaaa ccaagattta
    21061 ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc agcttctaaa
    21121 gtagaaggcg tatacggaat gcgcggaact tttgccagca atgtcactga attattagga
    21181 cgtgcagctc acggaaaagg tgtttactta gcaaacgagg aagatggctt gaaagtagat
    21241 ctttactgtt atctagaata tggtgtttca gttcctaaag tagcaatgga aatgcaggaa
    21301 cgggtcagcc agcaagtcct tttcatgacg gatatcgatt tatctgaagt caatatccat
    21361 gtagtagctg ttgttcctga aaaattagaa caaccaacaa tcgaagaact ttttgaagac
    21421 gaagaggaag agaatgaata aagaattatc aagacacgag attcgggaaa tggcattgca
    21481 ggctttattt ccattagact tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc
    21541 aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg ttcctgttta
    21601 tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat gaattggata aagtgattga
    21661 aaaacattta aaaaacaatt ggcacatcaa tcggatctca aaaatggatt tggtgatctt
    21721 gcgtatcgcg atttttgaaa tgacttatgt gacagatgta ccagcggctg ttgctttaaa
    21781 tgaagcaatc gagttggcaa aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg
    21841 tgttttatcc aatgtattga aagaattaga agctggggcg taagtcctag cttttttcta
    21901 tgaatcatca taaacctgtt ataaatcggg aaaattctat ggtagaatga aaacacaaaa
    21961 aatatctggg agtggttaga tggcagaatt gattaacgga aaagaattag ctgaaaaaat
    22021 gcaggcagag attgctgaaa aaatccaaaa attgaaagat aatggtatcc atcccggatt
    22081 agtcgtttta ttggtagggg aaaatccagc cagccaaatc tatgtaagaa ataaagaacg
    22141 tgtagcaaaa gaaatcggaa tccattcatt ggtagagcgt tatcctgaga cgatttctga
    22201 agacgaacta cttgcagaaa ttgaaaaata caatcaagat cctagttttc atggtatatt
    22261 ggtccaatta ccattgccaa agcatataga tgaagaaaag atcctgttag ctatccgccc
    22321 tgaaaaagac gtagacggtt ttcatccgat gaatttaggc agactccttg cgggtaaacc
    22381 ggacaagatt ccttgtactc catacggcat catgaaaatg tttgaagctt attctatcga
    22441 tccggcgggt aaacgagcag tagttatcgg acgaagcaat atcgtcggta aaccgatggc
    22501 tcagctactc ttgatggcag atgcaactgt tactgttgct cattctaaaa ccgaaaatct
    22561 ggctgaactg gcacaagaag cagatatttt agtcgtagca ataggacgtg gacattttgt
    22621 cacaaaagaa ttcgtaaaac caggtgctgt agtgatagat gtagggatga atcgtgatgc
    22681 taacggtaag ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg caggctggat
    22741 cactcctgtg ccaaagggtg tagggccaat gactatcacg atgctgatgt atcaaaccgt
    22801 aaaatctgcc gaagcttttg gagcaggtga atagatggca caagaatatt taactgtcac
    22861 tacattaacg aagtatttga aaagaaaatt tgatgccgat ccttatttag aacgcgtata
    22921 tttaacaggc gaaatatcga atttccgttt acgtgccaat gcccatcaat atttcagctt
    22981 aaaagatgac catgcaaaaa tatctgctat tatgttcaaa ggagcattcc aaaaattacg
    23041 attccagcca aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc
    23101 gggaggttct tatcaaatct atgtagagca catggagcct gatggaatag gtgctttata
    23161 tcaagaattg gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc aagggcctaa
    23221 aaaaatgctg ccacgtttcc caaagcgaat cgcggtcctt accagtccaa gcggagcggt
    23281 tatccgcgat atcatcacga cggtgaaaag aagatatcca attgtccaat tggttttgtt
    23341 cccaacagtt gtacaaggaa accaagcagc ggacaatgtt gtcaggaata tccagagagt
    23401 agaagagatg ggcaatttcg atacaatgat catcggtcgt gggggtggtt cgattgagga
    23461 tttatggccg tttaatgaag agcgggttgc tcgggcgatt tatcaagcta atacgccggt
    23521 gatttcatct gtaggacatg aaacagatac cacgatcgct gatttagtag cagatgttcg
    23581 ggctgccact ccaacagcag ctgcagaatt agctgttcct gttttgtcag aagagttgat
    23641 gcgcattcaa gaacggcaag ctagattgca gcaggcgttt gtacgccaga tccagcgcaa
    23701 acaagaacgg ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg aaagattgta
    23761 tgaaggtcag accatcaaat tagaccagtt gaaacagcga ttatatcaag ctacacagca
    23821 aatttatcat gcgaaagaaa aacaaacgat cgcattaagc caccaattgg aacaagtggc
    23881 tccgctttat cgagttaaag cagctcgcca agaaactgct tatctagaaa aacgcttaga
    23941 agagaaaatc caacaatata tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc
    24001 tcttgatttg ttgagtccat tgaaaatcat gggaagaggc tacagctata cgacgttaaa
    24061 tgatcaagta gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga cgatccatta
    24121 tcaagatgga atcgttaaag gacaagtaca agagatagac aaaggagaat aaaaatatgg
    24181 aaaatccaac ttttgaagag tcactgcaag aattagaaaa aattgtcatg cagttagaac
    24241 aaggagacgt tccattagaa tcagctctag attcgtttaa acgaggaatg gaattaagca
    24301 aacattgcca agatacatta gctaaagcag aagagacctt gacgaaaatg atgacagaat
    24361 caaatgaaga agttgatttt gacggaaacg aggaaagctg agatgagatt aactgacttt
    24421 tcagctattc atctacctgc tgtagaaaaa gaaatcttgt cttttttaga tgagcataca
    24481 acggatagag gactctatga tgcgatgagt tattcagtga aagcaggcgg gaaaagaata
    24541 cgtcccttat tacttttaac ggccgttgct tcctttgatg agcctattga tgttcccgtc
    24601 tatcaagtag cagctgcttt agaaatggtg catacgtatt ccttgatcca cgatgatttg
    24661 cctgcgatgg acaatgatga tcttcgtaga ggaaaaccta ccaatcataa agtattcggc
    24721 gaagcattag ctatattggc cggagatggt ttgcttacag gagcattcca gctaatcagt
    24781 atggcacact taggtaattc tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg
    24841 ggatcacaag gtatggttgc tgggcaagct gcagatatcg agggcgaatc gaaaaagctt
    24901 tctttagaag aattagcatt tattcatgaa agaaagacag ggcatttgat tcgttatgcc
    24961 ttgctggctg gtggcatatt agcgaagcaa cctgaagaaa tcttattgct acttcaacga
    25021 ttagccgaac atttaggttt agcttttcag ataagagatg atctactcga tgtgattggt
    25081 acaacgaaaa cattagggaa aacagctggt aaagatgaac gtatggaaaa aaatacttac
    25141 ccgcgtttat taggcctaga aaagacacgt gaggcgttgg agattgagct gttatccgcc
    25201 aacaagatca tagacaaatt agaagaggac gttttttctt ttgatggcgg gctgatgcgc
    25261 cagatgatca agcaatttga tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag
    25321 atgtattagc ggtcaaacaa ggattatttg aaacaagaga acaagccaaa cgttcagtga
    25381 tggctggttt gatttacaac gaaaaaaacg agcggtttga taaaccggga gaaaaaattc
    25441 cagtgagtag tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct cgagggggat
    25501 taaaattaga aaaagcactc aaagcatttg atctatctgt tgaaggaaaa acattgttag
    25561 atattggtgc atccactggc ggatttactg atgcagcttt gcaaaatggc gcaaaaatga
    25621 gttatgcttt agacgttggc tacaatcagc tagcttggaa aataagacaa gatcctagag
    25681 tcgtcgtaat ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt accttaggaa
    25741 ttccagaagt tgctgttatc gatgtttctt ttatttcact aaaactgatg ctgcctccat
    25801 tacatgctat tttaaaagaa gagggagaag tgattgcttt aatcaaaccc caattcgaag
    25861 caggcagaga agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag
    25921 ttttagaaga cattttgtca tttgcggcac aaggtggata tgatgtgctg gaattaagtt
    25981 attctccgat cacgggggga gaaggaaata tcgagttctt ggctcacttg aggaaagtac
    26041 ccgaaagcgg aacaatcaat tcagctatta atatggcaga agtggtttca aatgcacacg
    26101 aacagtttga ccataaataa attgtaactg tgaaaatcaa gtgagaagta tgcggattct
    26161 tctttattat accaaggaaa taagctatca ttgattaaga agaatatgct aaggagcgaa
    26221 gacgtaatga gaaaaaaaga tcggcatcgt ttgattactc gattgttgag tgagcaagat
    26281 atccgaaagc aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa
    26341 gctacgattt ccagagatat aaaggaactg aaactgatca aggtaccggc ttcagatggc
    26401 ggctaccgtt atagcatacc agctgaaaca aacgaagatg taaatactaa attggaaaaa
    26461 ctattaaaag acgctttcgt ggcagttgac caaatggaaa aatttgttat tttgaaaaca
    26521 ttgccgggaa atgcttcggc agcagcaaat ttgattgata aacgttacaa gaaagaatta
    26581 ttttcgatta tcaatgatga cgataatgtt ttgatgatta cacgaactga agaccaggca
    26641 gttttattga agaaagactt cctgcattac ctttaagttg aggagcgaat gtgatgctac
    26701 aagaaatcag tattactaac tttgcgatta ttccggaatt gcggctttct tttcacgaag
    26761 gaatgaccgc gctgaccgga gagactggtg cgggtaaatc cattattatc gatgctttag
    26821 gtctgttagc aggaggaaga ggttcaagcg attatatacg tcaaggagca gaaaaatgtg
    26881 tgcttgaagg gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg atggttgaat
    26941 tagggatcga aacagatgag gacaatctga ttgttcgacg ggatatgtcc ttaactggaa
    27001 aaaatgtttg tcgagtgaat ggtcatatta tcacattagc taatctgaga agaatcggca
    27061 gttatttagt agatatccaa ggacaaaacg aacatcagga actgctccag cctgaatcac
    27121 atcttgcttt attggatcgt tttggagatg tagctttcca acagaagaaa aagagttatc
    27181 agcaagaata tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg
    27241 aaaaatccta tgttcagaga atagatatgc tccatttcca gcaagaagaa atagctgctg
    27301 ctgatttgca agtcggagaa gaagagaaat taaaagaaga aagagaaaaa cttagcaatt
    27361 atcaaaaaat tgctgatgga ttagctgcag gttacggagc actgacgaat aatgaacaaa
    27421 acagtgtaga tggtgttggc ttggccgttt cagaactgca agggatcgcc catctagatg
    27481 tggagtatga agcaatttat gaaaatatcc aaagtgctta ctatttgctg caagatgcaa
    27541 tcggcgatat gagtcgtcag attgatttat tggagttaga tgagagtcgt ttagaagaag
    27601 ttacgcaacg tttagaatta atccgacagt tgaaacgtaa atacggcgaa tccattgagt
    27661 caatattggc ttactatgac gaaatcaccg aagagctagc ttcttctgat ttttcagaag
    27721 gtcaattgga caaaatgaaa gaggaactag agcaaaaaga gcttctactt cagcaacaag
    27781 ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc
    27841 atgaattgaa aagtctgtat atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag
    27901 aaaatagaca attgaatcat gatggatttg accaaatcga attttacatt acgaccaatc
    27961 caggagagcc actgaagcct ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt
    28021 tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt gacaagtatt atttttgatg
    28081 aagtggatac aggtgtgagc ggaagagttg cacaggccat cgcagataaa atttctcaaa
    28141 tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg
    28201 actatcaata ttatattgtg aaagcagtga ttgatggacg tacgcaaact tctgtttcag
    28261 agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga agtgagatta
    28321 caaaactcac actcgaacat gcaaaagaac tattgaaact cgcaaaaagg acctgagcca
    28381 cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta tattttattt ttacatgaca
    28441 ctcattacag cagtcaatac aacagatcca atcgttaaaa tcagcattgt ccaaatgaca
    28501 aattttgtta ctttagcaaa agtacttgtt tgtttttttt cacgcattgt tttcaacctc
    28561 gtcattaatt agttgattca agtgtacaat cttttttcca tttccacaag agggcttttt
    28621 tataaaaact tccctttttt taaataatag gtagttacga tacaaataaa taatgttaat
    28681 agaactagcc atataaaggc atcatccctt tcagcaatag gtaacttaat gttcattcca
    28741 tagatactgc cgacgattgt cggtatagtt aaaataatcg ttaaagaagt taaaattttc
    28801 ataatattat tcagattatt cgatacgatt gctgaaaaag tctgattcat ttgttctact
    28861 agttttaatt gaatgcgaga agtagtcatt gcctgctctg tttccaccaa tacatcacgt
    28921 aaatgatttt tcaaagcatg attttcttgg aaaagtttat tttgcgctag ttctttcaat
    28981 gcatctaaat tagcagaagt cgcttctctg aaataaacga ggcttttttg gatatccatg
    29041 atttgataaa gttgtttgtt ttcagtggat acttgaagtt tggcttccaa agtatcgcat
    29101 tgagattgaa gcgtagctaa ataactgttg taagagagtg tgacctgcca aatgaattga
    29161 aagaagatat tgagattagg cgaaatatca ctttttggaa aggtctggct aaagactttc
    29221 tttaaaaaca gtggttcatg attcgtaatt gtgataagct tctttttggg cgtcaaaata
    29281 atagagagcg ggtatgtatc cacttgaaaa taaccgctag gacttgtttt agcaaaagga
    29341 tattgtaaca ataataacgc tggctttgtc aactttgatt gattgaattc ttctgtacgg
    29401 gaattttctg catcgtccaa tatggaagtg atgtaatctg acggaatttc aaattgatgt
    29461 gtcagctgat gaatctcttc ttgattagga cgttctacac aataccagat cgtttggtca
    29521 gtttcctttc cggtattacg cagtttctca ttatctaatg taaaatattt gatcatgatt
    29581 ttcctcctgt tgcctgtttc ttttattata acggataggg aattggaata catggaatgt
    29641 gtttgtaaga tatagaatat aactgcataa atttaaataa aatgaaacag ttgtgtaaag
    29701 tatatacagt atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac
    29761 accacttatc ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct
    29821 tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc
    29881 ttttttgtgg ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc
    29941 gtttttcttt atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta
    30001 ttatgaagaa attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt
    30061 gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa
    30121 atagaaaagc tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg
    30181 tttatgaata aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat
    30241 tgtggtagac taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat
    30301 tccaacataa tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac
    30361 ttggcgaaaa gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga
    30421 gcgaatggga aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac
    30481 gtacttttgt gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac
    30541 gaatcaatat acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca
    30601 tcggtgtatc taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag
    30661 cagcagaaga gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat
    30721 aggaagaggc tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac
    30781 ggtagatgga ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc
    30841 cggacatagt gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga
    30901 ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg
    30961 tatggtcaca tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt
    31021 agataaggta gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc
    31081 agaacgtgga tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc
    31141 tcctttatcc gcttatcacg taattaatga atattcctac catgaattag taaagatttt
    31201 cttccgctat ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg
    31261 aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc
    31321 agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat
    31381 tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt
    31441 gaaagtcaat ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa
    31501 aagtatgttt aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc
    31561 tgacgaattc caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga
    31621 agagctaaca gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag
    31681 aatgaaagag gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt
    31741 tacctgaatt ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa
    31801 tcattcttcc acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg
    31861 ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca
    31921 caaatatcag cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa
    31981 agcaagaaaa aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta
    32041 aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt
    32101 aaatgagtct taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga
    32161 acaatcgcaa aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat
    32221 ttgccgtccg tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg
    32281 aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt
    32341 atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac
    32401 tctcaaaaac atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata
    32461 aaatagcaga aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt
    32521 tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata
    32581 tctcccaaga aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc
    32641 tttattttgt cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta
    32701 ttggatacgc agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt
    32761 ctgcttacaa tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt
    32821 ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata
    32881 tctacacgac tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa
    32941 atgaagaata tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa
    33001 tcttggcaat gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa
    33061 aagatgctat ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga
    33121 aagttttcac tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc
    33181 aatctggaaa aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag
    33241 gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg
    33301 aacagcgatt aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta
    33361 ctcattctgg attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc
    33421 gagcaatgag tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt
    33481 tcacttctat tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg
    33541 atccccaaac tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa
    33601 aagaaacaac agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg
    33661 gtagtgctta tggtgtatat agtgtacctg gatacaatgt atcggcaaaa accggaacag
    33721 cccaaatcgc ttctgataca gggggatatc aaacaggaga tacagcctac ccgtattcga
    33781 ttgtggagat ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc
    33841 ctaagacata tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa
    33901 tggattttaa agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg
    33961 tagccgatta tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac
    34021 tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg
    34081 atcagttgat ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg
    34141 acgtaacagg atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag
    34201 ttagttttga tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca
    34261 ctgaagacaa acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc
    34321 atggaatgga cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca
    34381 atgccgctat ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag
    34441 ggaccaaagt ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg
    34501 atcggctcta ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact
    34561 cttttcatct tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt
    34621 ataaaaatat tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag
    34681 attctagggg gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg
    34741 aatttcttcg gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg
    34801 cttgttggat tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc
    34861 ttaggaagta tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat
    34921 gtattaatca tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga
    34981 aaacctgcca agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg
    35041 gcaatttcta taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt
    35101 atggaaacag caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt
    35161 tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt
    35221 gatacagttt tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg
    35281 taggagatga cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt
    35341 aggactcgca aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt
    35401 caccgttaat gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt
    35461 agggataaaa gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt
    35521 gatggtcaaa aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat
    35581 gggtattccg gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat
    35641 tgggattaca ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa
    35701 tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg
    35761 tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact
    35821 catgggaatc acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca
    35881 tattgattat catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa
    35941 catgacagaa aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc
    36001 gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt
    36061 gtatgctgac gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact
    36121 tggcgtacca ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt
    36181 gtatggtata tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca
    36241 tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac
    36301 caatatttta gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt
    36361 ggctggcgga ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat
    36421 caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc
    36481 aggcatcgag acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga
    36541 aaacagtaca gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata
    36601 cccaaatttt gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa
    36661 aaagtagaag cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac
    36721 atatttatcc agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat
    36781 tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc
    36841 ctttccatac cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga
    36901 cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac
    36961 cagatatcgt tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca
    37021 aaatgggtat tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat
    37081 ttttaagccg atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc
    37141 ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa
    37201 aatcagatat tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg
    37261 gcggtagtca aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata
    37321 ttccttcttc ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa
    37381 ataaaataaa tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag
    37441 aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat
    37501 tcacagctct ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc
    37561 agactaaaaa tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg
    37621 aactaacagg tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc
    37681 aaaagcaaat gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt
    37741 atgacttagt aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa
    37801 agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa
    37861 ggctaaccga aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga
    37921 cataacatta gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca
    37981 gcaagaagaa agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa
    38041 aaacggtgga ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg
    38101 gcacaaagta ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat
    38161 ttttttgatc tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa
    38221 taagacagtc aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt
    38281 ttggggacag tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag
    38341 aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga
    38401 atataaagag attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg
    38461 aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt
    38521 caaagactct gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt
    38581 acaaaaagca atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca
    38641 attgaatatg aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat
    38701 gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt
    38761 tggtatcttc tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga
    38821 agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga
    38881 aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga
    38941 ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga
    39001 aaattgataa aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg
    39061 tagaattgga agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg
    39121 ttcgcttgta tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg
    39181 tatgtaggcc ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac
    39241 agccaaatga atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt
    39301 atcgttgata tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa
    39361 aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta
    39421 gaaaattgcc agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat
    39481 gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg
    39541 tcaatattgc cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga
    39601 atgatcggcg taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc
    39661 cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact
    39721 cctttagcgc tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc
    39781 attgatatgg gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt
    39841 accaatcttg accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat
    39901 acgtcattta acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg
    39961 acatcaccag atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc
    40021 gatgaacgct acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca
    40081 aaagatgcat tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc
    40141 ggagctgcta gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta
    40201 aaattgtatg ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt
    40261 atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg
    40321 ggtgaaacct cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag
    40381 agctatgaag cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa
    40441 aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga
    40501 ggaataagat atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt
    40561 catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa
    40621 aggtgttgaa ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga
    40681 aacagttatc caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga
    40741 agttggacaa aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc
    40801 agatatgatt ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat
    40861 cgttgcaggt atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt
    40921 cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga
    40981 aaatgtggat acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa
    41041 gacaccgatg cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg
    41101 tatctctgat ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac
    41161 agtgatggaa aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg
    41221 cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg
    41281 agctgaacaa gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca
    41341 agatgcttca gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac
    41401 atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga
    41461 tgaatcaaaa aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc
    41521 tgctcaaaag actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa
    41581 cagttttggc gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc
    41641 caattttgac aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc
    41701 tcatggcgat gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg
    41761 aagagcacat gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg
    41821 ctcttgttca tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat
    41881 ccgctcaagc tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag
    41941 ataaattatt agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa
    42001 ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg
    42061 cgttggacag tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct
    42121 gttttattga agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc
    42181 ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga
    42241 ctatggcgcc taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag
    42301 aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta
    42361 tggggatgag tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag
    42421 gaacagcgct attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga
    42481 gcaattgcta attttttcgg attagctgat gaggaagagt acgaatacca agattatcct
    42541 gaaacaacaa aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact
    42601 cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta
    42661 acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct
    42721 acaaaaactg tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat
    42781 cttgctggaa aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct
    42841 aaacgaatca ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa
    42901 gcgagaagaa tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa
    42961 cgtgttggag atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc
    43021 gctcaatctt ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata
    43081 ctattgcact attacttcat ttacttcaac gggcagccta tttctatagc attatcttga
    43141 tggtatatgc attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat
    43201 tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac
    43261 ccttggattt cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag
    43321 cacagatcgt tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt
    43381 attctcagaa tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg
    43441 cagatgaacg tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat
    43501 atgcacccta tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga
    43561 ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa
    43621 aaagatgttt gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat
    43681 tgtatgaaat ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa
    43741 ctttagtggg aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc
    43801 gctggcaagt atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga
    43861 ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag
    43921 atagctggac acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg
    43981 agatttataa tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag
    44041 tgaactggac agaaaataca agacctgatt ttgtacttga actattagat attatttcag
    44101 tcagaggtta tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt
    44161 acagggtact gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt
    44221 aactccatta gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga
    44281 cgaagtagat gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa
    44341 tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt
    44401 aaaagatgct ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac
    44461 aagtgcaagc aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt
    44521 agttgcgaac gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag
    44581 aaaaatcttg acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct
    44641 taagaaaaaa acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga
    44701 acaagtgaaa agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga
    44761 cagccatgaa gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga
    44821 tgtaaactca gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat
    44881 tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta
    44941 ttcttaaagc gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct
    45001 ctacttgaga cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg
    45061 tcacagagag tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg
    45121 tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg
    45181 aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt
    45241 gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag
    45301 acaagagttg aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa
    45361 tggtaatatc catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc
    45421 aaaatcaatg tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc
    45481 gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata
    45541 cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa
    45601 acgcctagga gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga
    45661 agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt
    45721 gaaaccaatt tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata
    45781 taaagatgta aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat
    45841 tcttgatgaa gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa
    45901 ccaaggtatt gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa
    45961 gtttgtcgta gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt
    46021 aacagttcta aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt
    46081 ctatgaacgt gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg
    46141 attagtccat actgctccag gacatgggga agatgactat atcgctggta gcaaatataa
    46201 actatctgta gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt
    46261 tgaaggtatc ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg
    46321 agctttgctg aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa
    46381 gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa
    46441 tattttggat gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta
    46501 taatatgatc cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc
    46561 acttccgatt ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca
    46621 tgtggcaaaa ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga
    46681 acttcttcct gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa
    46741 agatatcatg gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg
    46801 accagaatta tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg
    46861 gttcaactca agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt
    46921 tatttctcaa ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa
    46981 catcattgca ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt
    47041 atctagtgta gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc
    47101 agaagtatat cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt
    47161 cgatcctaaa aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac
    47221 cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt
    47281 catgcatatt taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct
    47341 ggattttgct aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat
    47401 gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc
    47461 gcatacagca gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag
    47521 cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc
    47581 tttcatggac ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt
    47641 gattggtaaa tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat
    47701 gttaaaagct ttggatgcag atattgcaca attattgatc gtttcaccag actactttga
    47761 agttatggca gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat
    47821 tacagtggaa aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt
    47881 aggagtagat gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt
    47941 ctatcctgaa gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac
    48001 atgttctttg tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc
    48061 agttatctgg ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt
    48121 taggtcccat tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt
    48181 ttcgaatgac atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg
    48241 ttccatcacc attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa
    48301 ccatttctgc actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta
    48361 cttctttccc attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa
    48421 cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa
    48481 caggaacttg tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg
    48541 tacgtacata gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga
    48601 cgaatgtttc agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt
    48661 gtacaccatc caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt
    48721 cttcgttata aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt
    48781 cggaaaatgc tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt
    48841 gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga
    48901 ggttttcgcc aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa
    48961 aaatattatt ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat
    49021 gatcaatcct gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa
    49081 aagcagactg gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt
    49141 ttgtcataat attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca
    49201 tagccaaata atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca
    49261 attcttttaa tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat
    49321 ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga
    49381 caacttcacg gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat
    49441 gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg
    49501 ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga
    49561 tacactcctt tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat
    49621 tttctttcta tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc
    49681 atattcttcg ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc
    49741 aagcactcgt gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg
    49801 ttcttcatgg acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg
    49861 atgattcaaa tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac
    49921 ttcagcatca tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc
    49981 ccataagcga tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg
    50041 tacaccttga taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc
    50101 actgacagat gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt
    50161 tgtatctcca cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt
    50221 cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt
    50281 gactattttt ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa
    50341 gatgatagag aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat
    50401 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
    50461 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
    50521 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
    50581 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
    50641 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
    50701 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
    50761 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
    50821 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
    50881 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
    50941 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
    51001 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
    51061 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
    51121 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
    51181 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
    51241 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
    51301 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
    51361 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
    51421 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
    51481 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
    51541 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
    51601 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
    51661 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
    51721 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
    51781 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
    51841 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
    51901 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
    51961 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
    52021 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
    52081 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
    52141 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
    52201 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
    52261 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
    52321 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
    52381 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
    52441 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
    52501 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
    52561 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
    52621 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
    52681 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
    52741 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
    52801 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
    52861 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
    52921 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
    52981 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
    53041 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
    53101 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
    53161 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
    53221 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
    53281 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
    53341 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
    53401 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
    53461 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
    53521 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
    53581 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
    53641 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
    53701 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
    53761 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
    53821 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
    53881 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
    53941 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
    54001 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
    54061 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
    54121 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
    54181 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
    54241 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
    54301 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
    54361 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
    54421 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
    54481 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
    54541 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
    54601 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
    54661 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
    54721 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
    54781 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
    54841 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
    54901 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
    54961 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
    55021 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
    55081 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
    55141 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
    55201 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
    55261 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
    55321 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
    55381 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
    55441 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
    55501 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
    55561 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
    55621 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
    55681 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
    55741 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
    55801 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
    55861 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
    55921 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
    55981 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
    56041 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
    56101 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
    56161 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
    56221 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
    56281 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
    56341 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
    56401 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
    56461 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
    56521 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
    56581 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
    56641 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
    56701 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
    56761 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
    56821 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
    56881 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
    56941 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
    57001 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
    57061 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
    57121 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
    57181 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
    57241 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
    57301 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
    57361 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
    57421 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
    57481 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
    57541 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
    57601 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
    57661 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
    57721 atgcttttat cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
    57781 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
    57841 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
    57901 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
    57961 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
    58021 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
    58081 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
    58141 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
    58201 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
    58261 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
    58321 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
    58381 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt
    58441 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
    58501 gaccaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
    58561 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
    58621 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
    58681 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
    58741 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
    58801 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
    58861 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
    58921 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
    58981 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
    59041 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
    59101 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
    59161 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
    59221 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
    59281 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
    59341 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
    59401 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
    59461 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
    59521 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
    59581 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
    59641 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
    59701 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
    59761 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
    59821 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
    59881 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
    59941 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
    60001 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
    60061 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
    60121 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
    60181 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
    60241 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
    60301 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
    60361 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    60421 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    60481 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    60541 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    60601 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    60661 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    60721 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    60781 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    60841 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    60901 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    60961 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    61021 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    61081 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    61141 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    61201 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    61261 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    61321 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    61381 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    61441 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    61501 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    61561 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    61621 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    61681 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    61741 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    61801 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    61861 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    61921 atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
    61981 aagtatattt actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa
    62041 agtaaattgc ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc
    62101 gtttgatgaa actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat
    62161 ttcaaaattc tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt
    62221 atgtttatca atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt
    62281 taatttataa ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc
    62341 ccatgttaat cctagaagtt cagcaaatcg cattccagtg gcaccagcaa ctaacacgag
    62401 cataggagaa gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa
    62461 ttgatcataa tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac
    62521 tgcttttcta gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc
    62581 tctaatatgg ttattaaact taataacggt agattttaca tggtctttag catacttatt
    62641 taaaaagcgt tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt
    62701 ctttatgttc attaacgtat cttcgtattt tctataagtg ataggagata ctgttccttc
    62761 tttataaagt tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc
    62821 tttaaggcct ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt
    62881 tgtcttaaat cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat
    62941 ttcgtattgc caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt
    63001 tctctgatac aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac
    63061 atcctttttg cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat
    63121 gtgtattaga tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata
    63181 actgaacttc cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt
    63241 gcattgctga taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa
    63301 taaattgaat tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta
    63361 atactatgga gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac
    63421 catctattag ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc
    63481 cacgactcat taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa
    63541 gaagataata ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa caattcttat
    63601 aaagattttt atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta
    63661 tcccttcgaa tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa
    63721 tttgatcgcg aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt
    63781 tgaaacgctt ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag
    63841 gaattgctcc aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt
    63901 cattgctatt atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc
    63961 ctgatggcac tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg
    64021 tggttcattt attttttact tactttacta ttaatcatat cccattgagc ataggatttt
    64081 tctatgttcg atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta
    64141 aaggtatcag cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca
    64201 gaaatatcat tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta
    64261 gtcattgatt cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta
    64321 aaagctgtat tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc
    64381 cagtcatgat tatttgtctc taaaaattct tcaaattgag ttaggctttt attgctgctg
    64441 ttagatgtag tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc
    64501 tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa taatcccgat aataaggaga
    64561 atcataggtg ttttcttatt tgtttttttg ataaatctta caattaacat gattaaacct
    64621 ataataaata aaacaatacc agtaaaacca aatataccgt ccattaatag atcctctttc
    64681 tcttttgata ttgaatatta taaaccaagc aattcttttt tcttaaaatc gaattcttct
    64741 tgattaataa tcccttcatc taacaactct ttatactttt taatttcatc tgcacttgaa
    64801 atttgtgttt ctttggaatt attatctaat atagattgtt gcttctctat atattttttt
    64861 aaattttcca tttcattcca atattttttt gtaaacataa cggtgttttc atctttcgtg
    64921 gccgctaata tacctccgcg actttcgttt ccgcctagca aggtaaattg tatataacca
    64981 ttactcatac ctggtttttt taattgtacg gctgatatat ttttaaaggg gatagttttt
    65041 tctcctttaa taccttgatt aactaaattt ataaatcctt ttctaataat tgaaatagaa
    65101 tcatcagtta ctttaatcaa ggtttttcct ggagatttta ttaaaatctc tttttccatt
    65161 ttttcttcct catttctgtg atatgatttt ttttgtagaa gctcatatat gaggaaagag
    65221 cccgtgttgc agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct
    65281 tgatgtattc atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag
    65341 acagcataac aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg
    65401 agattaaact gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga
    65461 atagctattt tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac
    65521 aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag
    65581 gcttctgcag tttcaccgta tagattttca ttcaaaaaaa tgattctggg ttttggatag
    65641 tagaaaccag gctctttcat ctccatataa actactttca aattgtattc gctcaacatt
    65701 tctttcaatt tcaaatacat acaaaccatc actccaacta ttcattttcc tctaaagctt
    65761 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
    65821 aaacagtatc gtcttctgat aaatccacat gtttaggggt ttctctttct tctctaccta
    65881 gaaggtagtc tacagagaca tcgaaatagt cagcaatccg acttaattca tctgaattag
    65941 gagtattatt tttccacttt gctagataac cgttagaata tccaaaatta atttctaact
    66001 gccgtataga tatctttttc ctcttcgcca agtcttttat tatttcatag gtattcatcg
    66061 ataaaacaac ctttctaaat gtttacaaaa aaagtttaga aaaatacgca gaaataattt
    66121 gactaattct gagtaatgag ctatactata tcttgtaaac agatttaaca actaaaaaga
    66181 caacaaaaaa cactattgat atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt
    66241 ttttatgtct tatttaatta tgctcttagt atagagtatt actcagatac tgtcaattga
    66301 atttagaaaa aagttgttaa atttgtttac gaatataaaa gaaaggaatg agaaatatga
    66361 acacaccgca aatttttaat ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg
    66421 aaccatattt tgtaggcaaa gacgttgcga gtgttttggg ttattcaaac actaaagatg
    66481 ctttgtcgcg gcatgtagat ttggaagata agatggggtc gcgaatcacg acctcaggtc
    66541 aatcaagaga gatgacaatc atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa
    66601 aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta
    66661 gaaaacatgg aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata
    66721 caatcattca attagcaact caactaaaag aagaaagaac tggaagatta atcgcagaac
    66781 aaaagattgc agagtacgaa cctaaaatct cctatttaga tagcatatta tcttctacag
    66841 attcagtaac aattagtcag attgcagcag attatgggat gtctccacaa cagatgaata
    66901 aattacttca taaactaggt attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa
    66961 aacacatgaa ccaaggatac acaaaatctc atacaactga gatcccgaaa gccgatggtg
    67021 gcactaaaat tgtaatgaat accaaatgga cacagaaagg gcgtctattt atctacgaat
    67081 tactaaaaaa agaaggatat taccctcaaa tggatttaga ggaaattggt tagaaaggag
    67141 ttttagtatg actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata
    67201 tgtcgaaagt tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa
    67261 aagctactta tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat
    67321 agattcgatt atcactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga
    67381 agcaaaaatc ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt
    67441 aaaaaaagct gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa
    67501 cagaaaatat gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat
    67561 cgatatcgag aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata
    67621 tctattttta agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag
    67681 gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct
    67741 acgctttagg actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt
    67801 ggatttttat ggggcttgat gaagctgaag ctgagtacag agcgaaaaag ccaaccgagg
    67861 gggctgacta aaaatgaata gaaaagagaa actagaatgt atattactat tactcagttt
    67921 aattctttca ctaatttctc tattgggaag tttgtatttt tgatatcaac aattttgtgg
    67981 atttgggttc tatttagagt aaatatcatt tttgtttggt gattaaggag tttattaaaa
    68041 ttaattttcc ctacttcaaa tgcaaggtaa caagatatag cacttttagg aggaatattt
    68101 agcggtacag cttcagagta tattctatta tcatcgactg atcctccttt agcaattaat
    68161 ttcttaaatg gagtagctga ccatctgcgg tttaagttag cggatgaaag ttctaaatta
    68221 actaacatag ctggttctgt tgaatagtta gaaatgatta cttttgtata aatcatatct
    68281 gcagcgaagt aagaagcatt aagttcaact tctagttgag ggcgcattcc ttttattttt
    68341 atccctgtta aaaacgtact caatataaaa cttattattg acatccattg aaaaattgtt
    68401 aagtgaaaaa aattcaaaat aaacaccacc agtttttaac taaattatac caaaaaggag
    68461 agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagctt
    68521 acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact
    68581 aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac cagattcata
    68641 tggcagaaat cgacgagttt tcttcattag tagagatgga tttttcttgc tggctatggg
    68701 gtttacagga aagaaagcta tctacttcaa acaaaaatac attgaagcat tcaacgaaat
    68761 ggaagatgtt attcgcaaga atactgttcc tcaaacaatt gaagatatga tgatctatca
    68821 attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat
    68881 tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta tgggagttct
    68941 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
    69001 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
    69061 cgatgatgct gtttcattta ttgaaatgtg gttgccagaa actgcgatcc gtatggaaat
    69121 tgatcaactg aacagacaac agagactttt tggtgatgac aatgaataga gctgaagcgc
    69181 taagaatagg gacggcaatt gctaattgct ggtggaaata ctataaacca atcatcctaa
    69241 gccaacaaca tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc
    69301 aagcaaagag tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact
    69361 ttccactcta gatcaatatt tgattgatcc tgattggggc aagccgaaaa ttgaggaaac
    69421 aagtggtcga aaaatcagac gaaatctttt gacgaatgaa gaactagctt gggatcaaga
    69481 tgatttaggc aaccatgtaa ctatttggga tcatgtttat cttatccatc tatcgaagca
    69541 ttcgaataaa cctgaatata tttacgtcat cgaagatggc ttgattgatg cgctagaaga
    69601 gtacgacaga gataacttga ttgatatctc ttattacgga ccaggtaaga aatacattgc
    69661 tgaaatggag gcagaatttg atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt
    69721 ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa ttttgattat ttatcttggg
    69781 ctcacgcgtg ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt
    69841 acaaacacta cagggttgtt tctggaactc atcaagactt tcttgttgag gaatataaac
    69901 cttttcttat ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca
    69961 cggaaaccga attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg
    70021 caatgcaaat caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac
    70081 tgggattata tgtatttcaa ggggaagata ttccaacacc acctagaatc gatacaaaga
    70141 aattaaacat gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga
    70201 ccaaaacctt aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata
    70261 acgttgaaac tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag
    70321 cagctaagag aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc
    70381 atattcagcg gttctgaaaa agttcaaagg aaaagacata agcgcaacca tcaatgagga
    70441 agtgaacatt gatcgactaa agacgatgta tgacg
//
LOCUS       DACCSK010000003        60822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCSK010000003 DACCSK010000000
VERSION     DACCSK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.003"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            155..640
                     /locus_tag="IVP39_000155"
     CDS             155..640
                     /locus_tag="IVP39_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7585262.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            633..1316
                     /locus_tag="IVP39_000156"
     CDS             633..1316
                     /locus_tag="IVP39_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7585263.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1442..4087
                     /gene="polA"
                     /locus_tag="IVP39_000157"
     CDS             1442..4087
                     /gene="polA"
                     /locus_tag="IVP39_000157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7585264.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4132..4968
                     /gene="mutM"
                     /locus_tag="IVP39_000158"
     CDS             4132..4968
                     /gene="mutM"
                     /locus_tag="IVP39_000158"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7585265.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4932..5561
                     /locus_tag="IVP39_000159"
     CDS             4932..5561
                     /locus_tag="IVP39_000159"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7585266.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5613..5945
                     /locus_tag="IVP39_000160"
     CDS             5613..5945
                     /locus_tag="IVP39_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7585267.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6257..6748
                     /gene="nrdR"
                     /locus_tag="IVP39_000161"
     CDS             6257..6748
                     /gene="nrdR"
                     /locus_tag="IVP39_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7585268.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6771..8144
                     /locus_tag="IVP39_000162"
     CDS             6771..8144
                     /locus_tag="IVP39_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7585269.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8144..9070
                     /gene="dnaI"
                     /locus_tag="IVP39_000163"
     CDS             8144..9070
                     /gene="dnaI"
                     /locus_tag="IVP39_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7585270.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9199..9639
                     /locus_tag="IVP39_000164"
     CDS             9199..9639
                     /locus_tag="IVP39_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7585271.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9783..10484
                     /locus_tag="IVP39_000165"
     CDS             9783..10484
                     /locus_tag="IVP39_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7585272.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10481..11602
                     /locus_tag="IVP39_000166"
     CDS             10481..11602
                     /locus_tag="IVP39_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7585273.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11617..12849
                     /gene="pepT"
                     /locus_tag="IVP39_000167"
     CDS             11617..12849
                     /gene="pepT"
                     /locus_tag="IVP39_000167"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7585274.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            12981..13889
                     /locus_tag="IVP39_000168"
     CDS             12981..13889
                     /locus_tag="IVP39_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7585275.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            13924..14196
                     /locus_tag="IVP39_000169"
     CDS             13924..14196
                     /locus_tag="IVP39_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7585276.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14207..15253
                     /locus_tag="IVP39_000170"
     CDS             14207..15253
                     /locus_tag="IVP39_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7585277.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15504..18113
                     /gene="clpB"
                     /locus_tag="IVP39_000171"
     CDS             15504..18113
                     /gene="clpB"
                     /locus_tag="IVP39_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7585278.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18264..18935)
                     /locus_tag="IVP39_000172"
     CDS             complement(18264..18935)
                     /locus_tag="IVP39_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585279.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(18928..19422)
                     /locus_tag="IVP39_000173"
     CDS             complement(18928..19422)
                     /locus_tag="IVP39_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7585280.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19442..20662)
                     /locus_tag="IVP39_000174"
     CDS             complement(19442..20662)
                     /locus_tag="IVP39_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7585281.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20809..22644
                     /gene="lepA"
                     /locus_tag="IVP39_000175"
     CDS             20809..22644
                     /gene="lepA"
                     /locus_tag="IVP39_000175"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7585282.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            22840..23751
                     /locus_tag="IVP39_000176"
     CDS             22840..23751
                     /locus_tag="IVP39_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7585283.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            24747..24998
                     /locus_tag="IVP39_000177"
     CDS             24747..24998
                     /locus_tag="IVP39_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585284.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            25081..25353
                     /locus_tag="IVP39_000178"
     CDS             25081..25353
                     /locus_tag="IVP39_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585285.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            25443..25682
                     /locus_tag="IVP39_000179"
     CDS             25443..25682
                     /locus_tag="IVP39_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585286.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            25829..30112
                     /locus_tag="IVP39_000180"
     CDS             25829..30112
                     /locus_tag="IVP39_000180"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP7585287.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            30211..30576
                     /locus_tag="IVP39_000181"
     CDS             30211..30576
                     /locus_tag="IVP39_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585288.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            30578..31030
                     /locus_tag="IVP39_000182"
     CDS             30578..31030
                     /locus_tag="IVP39_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585289.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            31644..32954
                     /locus_tag="IVP39_000183"
     CDS             31644..32954
                     /locus_tag="IVP39_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7585290.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLIKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            32977..33288
                     /locus_tag="IVP39_000184"
     CDS             32977..33288
                     /locus_tag="IVP39_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585291.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            33649..34905
                     /locus_tag="IVP39_000185"
     CDS             33649..34905
                     /locus_tag="IVP39_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7585292.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            34902..35447
                     /locus_tag="IVP39_000186"
     CDS             34902..35447
                     /locus_tag="IVP39_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585293.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            35448..35948
                     /locus_tag="IVP39_000187"
     CDS             35448..35948
                     /locus_tag="IVP39_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585294.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            36029..36585
                     /locus_tag="IVP39_000188"
                     /pseudo
     CDS             36029..36585
                     /locus_tag="IVP39_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36605..36949
                     /locus_tag="IVP39_000189"
     CDS             36605..36949
                     /locus_tag="IVP39_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585295.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            36968..37879
                     /locus_tag="IVP39_000190"
     CDS             36968..37879
                     /locus_tag="IVP39_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7585296.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            37958..38950
                     /locus_tag="IVP39_000191"
     CDS             37958..38950
                     /locus_tag="IVP39_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7585297.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            38947..39165
                     /locus_tag="IVP39_000192"
     CDS             38947..39165
                     /locus_tag="IVP39_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585298.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            39178..39588
                     /locus_tag="IVP39_000193"
     CDS             39178..39588
                     /locus_tag="IVP39_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585299.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            39640..42168
                     /locus_tag="IVP39_000194"
     CDS             39640..42168
                     /locus_tag="IVP39_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7585300.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            42184..44394
                     /locus_tag="IVP39_000195"
     CDS             42184..44394
                     /locus_tag="IVP39_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585301.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            44402..45493
                     /locus_tag="IVP39_000196"
     CDS             44402..45493
                     /locus_tag="IVP39_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7585302.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            45514..46056
                     /locus_tag="IVP39_000197"
     CDS             45514..46056
                     /locus_tag="IVP39_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585303.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            46278..46508
                     /locus_tag="IVP39_000198"
     CDS             46278..46508
                     /locus_tag="IVP39_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585304.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            46522..46854
                     /locus_tag="IVP39_000199"
     CDS             46522..46854
                     /locus_tag="IVP39_000199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585305.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            46899..47447
                     /locus_tag="IVP39_000200"
     CDS             46899..47447
                     /locus_tag="IVP39_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7585306.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            47454..47906
                     /locus_tag="IVP39_000201"
     CDS             47454..47906
                     /locus_tag="IVP39_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585307.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            48003..48338
                     /locus_tag="IVP39_000202"
                     /pseudo
     CDS             48003..48338
                     /locus_tag="IVP39_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(49063..49734)
                     /locus_tag="IVP39_000203"
     CDS             complement(49063..49734)
                     /locus_tag="IVP39_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7585308.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            50038..51669
                     /locus_tag="IVP39_000204"
     CDS             50038..51669
                     /locus_tag="IVP39_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7585309.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            51672..52751
                     /gene="uxuA"
                     /locus_tag="IVP39_000205"
     CDS             51672..52751
                     /gene="uxuA"
                     /locus_tag="IVP39_000205"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7585310.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            52825..54252
                     /gene="uxaC"
                     /locus_tag="IVP39_000206"
     CDS             52825..54252
                     /gene="uxaC"
                     /locus_tag="IVP39_000206"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7585311.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            54336..54983
                     /gene="eda"
                     /locus_tag="IVP39_000207"
     CDS             54336..54983
                     /gene="eda"
                     /locus_tag="IVP39_000207"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7585312.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            55103..56479
                     /locus_tag="IVP39_000208"
     CDS             55103..56479
                     /locus_tag="IVP39_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7585313.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            56489..57283
                     /locus_tag="IVP39_000209"
     CDS             56489..57283
                     /locus_tag="IVP39_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7585314.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            57296..59062
                     /locus_tag="IVP39_000210"
     CDS             57296..59062
                     /locus_tag="IVP39_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP7585315.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            59085..>60822
                     /gene="uidA"
                     /locus_tag="IVP39_000211"
     CDS             59085..>60822
                     /gene="uidA"
                     /locus_tag="IVP39_000211"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP7585316.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDR"
BASE COUNT        20848 a         9350 c        12813 g        17811 t
ORIGIN      
        1 ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct ctgtaaacta
       61 taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt ggtacaataa
      121 tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc cgagaaaact
      181 aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag aatctattga
      241 agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt tgtatatcca
      301 acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag tgatgctgat
      361 ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac atgttgtcaa
      421 tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt ttcagtttga
      481 ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag cggagaatac
      541 gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag ttcctgaatt
      601 ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca tcaatgtcag
      661 gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt gcccttagtg
      721 cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact tttatcaata
      781 attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta atggttctag
      841 gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc tactctcttt
      901 tgaatggcat aacattagcg gttactcttg catactacga tattggaaca gtaacgcaag
      961 ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta acaacaaaaa
     1021 gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg atcattgcac
     1081 tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc tccctactgt
     1141 tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt aatatctatg
     1201 tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg aagctgtatt
     1261 tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac aactaaaaaa
     1321 gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg ctttttcttt
     1381 tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag gagttaagaa
     1441 gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc gcgcattctt
     1501 tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata caaatgcaat
     1561 ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga ctcatgttct
     1621 ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg agtacaaagg
     1681 tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac gtgatttgtt
     1741 gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg atattatcgg
     1801 tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt caggtgatcg
     1861 ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa aaggtgtcag
     1921 cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt tgacgccaaa
     1981 acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc ccggtgtgac
     2041 taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag tcgaaggaat
     2101 ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga taaatgacaa
     2161 agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc ctgtggatgt
     2221 ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc cgttttataa
     2281 agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag aagtaaaaat
     2341 ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta ctacggatat
     2401 ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg tcggagtagc
     2461 gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg aaaaccgagc
     2521 gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg cgaaacgaac
     2581 ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg atgtcttgct
     2641 aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa tagctgctca
     2701 ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg cgaaaaaagg
     2761 tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag ctgcaatcaa
     2821 tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag atcttttccg
     2881 gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg ggatcaaagt
     2941 agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa gagagatcga
     3001 acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa agcaattagg
     3061 agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa ctggatattc
     3121 cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag aggatatcct
     3181 cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc tgaaagtgat
     3241 ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa caggacgatt
     3301 aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag ggagaaagat
     3361 acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg actattcaca
     3421 aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag ctgccttcct
     3481 agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg aaaaggcaga
     3541 ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta tcgtgtatgg
     3601 catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag cacagcaata
     3661 catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa gaatcgtacg
     3721 cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat atctgccaga
     3781 catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta tcaatacacc
     3841 aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg ataaacgatt
     3901 aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac ttgtcttcga
     3961 ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta tggaacaagc
     4021 tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt ggtacgaagc
     4081 aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa aatgccagaa
     4141 ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg aaaaacgatt
     4201 caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc catctttgag
     4261 gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt tttaattttt
     4321 catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata tcaatttacg
     4381 aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga ggatggaagc
     4441 caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga aaaaggagca
     4501 agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga tagcttctta
     4561 ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc attgctgcta
     4621 gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt atgggaagcg
     4681 aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga acaattacgt
     4741 ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac gattcgcagt
     4801 tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt ctatcagcaa
     4861 actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt ggcacaaagg
     4921 gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga tttgttttag
     4981 gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt cagtctcatg
     5041 gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct ggaacgcttg
     5101 ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat gggcaactag
     5161 atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa aaacttgatc
     5221 agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct ctaagttcca
     5281 atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat gaccactata
     5341 tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt ttgatgaaac
     5401 gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt ccaatcgaag
     5461 aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa aaacttgtgg
     5521 gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg tcaattttgc
     5581 tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat caaaaggata
     5641 aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa aatctaatcg
     5701 atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac caagcaagaa
     5761 tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat ttgactcaag
     5821 aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt gaccacattc
     5881 ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc gaagagaagt
     5941 tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt tcatctgctt
     6001 caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct cagtaatcaa
     6061 ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt acgcatgaaa
     6121 gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt ttgtgatttg
     6181 tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata aaatgcgatg
     6241 caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac gagtaatcga
     6301 cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg agaattgttc
     6361 gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa tcaagaaaaa
     6421 cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc gttcagcaga
     6481 aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg aaaaccgcgt
     6541 acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg tgatggaaga
     6601 tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc gccagttcaa
     6661 agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa aagaaaataa
     6721 taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc ttggaaaatg
     6781 cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct ccgctgagag
     6841 aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga gaagccttga
     6901 gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt cctgaaggga
     6961 tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat gatgcaagaa
     7021 aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt gatctgggac
     7081 ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc aaagactctg
     7141 ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag cttacccaat
     7201 tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa aaatttttag
     7261 atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc gaatcgctga
     7321 ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg acattcgatt
     7381 ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat gatccaatca
     7441 tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca cttggcgaac
     7501 tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa ctgcaaaaag
     7561 ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt gcggtttctg
     7621 aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt cagctgataa
     7681 aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca gaaaaaggcg
     7741 gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa tcaggtcttt
     7801 ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat aatgctagct
     7861 taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa atcgccacag
     7921 cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa tcaaaacaaa
     7981 agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag cttcctgatt
     8041 gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca gagattgacc
     8101 gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag acgtaggcaa
     8161 agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg aaatgatcgc
     8221 tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag aagaattgac
     8281 acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag aaaagcgtaa
     8341 atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc tgacgttgaa
     8401 ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac ggcaaagaca
     8461 agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc aatcggcttc
     8521 ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag ccctagattt
     8581 tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc tagtaggaag
     8641 ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag cgattgctgg
     8701 attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac aggcaatcgg
     8761 aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt tgatgatcga
     8821 tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg gtgttatcct
     8881 tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta caatggatga
     8941 attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa aagcgaaacg
     9001 gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc gcaaccgacg
     9061 caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa agcaggtcag
     9121 cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta atgcagaaac
     9181 ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt tggtctattt
     9241 tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga ttcgattgat
     9301 gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat atttgtcgga
     9361 acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt gaaactcgtc
     9421 gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa tgacggagtg
     9481 atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa aatcggtgac
     9541 aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc accgcaagtt
     9601 cctggagatt cgattattga tgtttcattg aacaaataac caataacatt ttttcaaaaa
     9661 taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata tgggaatttt
     9721 tttattccta gtctccttat tttatttgga aaatgctata attatctaga aggagtgaag
     9781 ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt gtccctgaat
     9841 ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca ttgatattaa
     9901 gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat gaatcagcac
     9961 gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt gcaaatggtc
    10021 tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt atgggaggtt
    10081 cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc ggacaagaac
    10141 gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg caagaaaatc
    10201 agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat gaaatcattg
    10261 tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg ttcgggccat
    10321 ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag cttcaacaaa
    10381 gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt caaaaacaaa
    10441 tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca gcggacgcac
    10501 tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag gagacccagt
    10561 cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga cattagacgt
    10621 tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga ttgctaaaca
    10681 tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag aaaaaatgta
    10741 tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca tggatattat
    10801 ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg agagttattt
    10861 agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg tggatcatgc
    10921 acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg attataccgg
    10981 tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg cacaaccagc
    11041 gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg ttattttgcc
    11101 ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt atgaggaacc
    11161 tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag gacgagtagg
    11221 tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag aagcgttcca
    11281 actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga agcgcattgc
    11341 gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa gagcagatgt
    11401 ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg ctgggttgat
    11461 tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta tcgagaaatt
    11521 tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg aaacaaatac
    11581 aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct tttgccacgt
    11641 tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac gactccatcg
    11701 actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga gttaggctta
    11761 gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc aagcaatgtt
    11821 gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga ttttaatgca
    11881 gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat cgtactagat
    11941 gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa ttatgaaggc
    12001 cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa atcagggatt
    12061 gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc tcacggtacc
    12121 atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa attcgatgtt
    12181 gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg tgagttgcag
    12241 tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa tgtacatcca
    12301 gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt tcataatcag
    12361 cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt ccatttgatg
    12421 gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga tcatgatcgt
    12481 gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt gaatcagcga
    12541 tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat gaaagaaatc
    12601 attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga gcttggtatc
    12661 tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc ttacatgggt
    12721 attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt cgaatttgtt
    12781 tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga attgaacaca
    12841 aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat gatggaatgt
    12901 catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct cttttttgaa
    12961 gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa agttcaggga
    13021 aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa aagcagccag
    13081 aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa ttagtcaaga
    13141 aagcagaagc catagaagct gatactttag taattatagg tggagacggc acgattcatc
    13201 atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta cttccaggtg
    13261 gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa gaagctgctg
    13321 acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat gatgaagtca
    13381 ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc agtcaagaag
    13441 aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta ttgctgaaga
    13501 agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga aaatcccaac
    13561 tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac gactcagcga
    13621 ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt ttccgattta
    13681 ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc attacttatt
    13741 tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact cgaacggatg
    13801 gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga ttaacagttt
    13861 attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt tggaagaaga
    13921 gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt gaatttttac
    13981 ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg gcgtgttcct
    14041 gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt gatctctcga
    14101 aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc tgcaggtgta
    14161 cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt cgtctaaagg
    14221 aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg ccattattta
    14281 ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta ccagtgttgt
    14341 tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag ggcgctcaat
    14401 caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt tcttgacctc
    14461 tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga tctcggagat
    14521 ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga cagcactcca
    14581 tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg tcatccaatt
    14641 ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg gtatcctttt
    14701 gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg ttcaaatatt
    14761 ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat gggtggatac
    14821 atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc aagacaggac
    14881 tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt atgtacttcc
    14941 ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca tttcgtttat
    15001 cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg gttcctttga
    15061 atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag tttcttcgct
    15121 tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg tgtatgtgat
    15181 tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag agaataaaga
    15241 aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag aaaaacgtta
    15301 tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa tcaattttgt
    15361 ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta tattttgacc
    15421 aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag tgatcttacc
    15481 tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg cttcaagaag
    15541 cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc gacattgccc
    15601 atgtatggaa aatcttttta caacctaatc attttggtcg aaatttctat acggatgccg
    15661 gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag tatccagttg
    15721 tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt cgattattga
    15781 atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta
    15841 ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat aaaaacggat
    15901 tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa agagtgacct
    15961 cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat ttagtacagc
    16021 aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca
    16081 tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa cccggtgtag
    16141 ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa
    16201 atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct ggagcaaaat
    16261 tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg
    16321 gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga aaaacagaag
    16381 gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga
    16441 tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa gcgttggaac
    16501 gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc agtattctgc
    16561 gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac aacgcattag
    16621 tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg gacaaagcaa
    16681 tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct atgccaacgg
    16741 aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct gcactgaaaa
    16801 aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac
    16861 ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt
    16921 ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa
    16981 ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct caattagaaa
    17041 aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg gttcaagagt
    17101 ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc cccgtaacga
    17161 aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt cataaacgag
    17221 tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg
    17281 gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca actggtgtcg
    17341 gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa gatcatatgg
    17401 tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta gtcggagctc
    17461 ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc
    17521 cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct
    17581 tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta gtagatttca
    17641 aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg gaaggcgtga
    17701 ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa
    17761 acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca ccattgagcc
    17821 tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa cgtctggaac
    17881 atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct gaaaatgctt
    17941 atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa gtagaaacac
    18001 cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta acgattactt
    18061 tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc taatcttcca
    18121 ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc cttttttcaa
    18181 ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct tgaagctgcc
    18241 cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt ataactttca
    18301 aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa ggcagcacct
    18361 atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt
    18421 gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc gcataagaat
    18481 tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa taagaggaac
    18541 aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat caatccatag
    18601 ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca aaaagagatg
    18661 atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa
    18721 aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg
    18781 aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg agaagtcgaa
    18841 tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc aaaagcaagt
    18901 aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat ctaaaaatct
    18961 ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca atttgatcaa
    19021 acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc
    19081 gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc cgatcactgg
    19141 gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga tcgtcagccc
    19201 atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag caagagcaat
    19261 cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt gaggaagata
    19321 gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt tatataacaa
    19381 atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat catccttttt
    19441 atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct agtgacttca
    19501 gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca cggcgataat
    19561 tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct ataggttcat
    19621 ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca ttattccaag
    19681 gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca aacggaaacc
    19741 gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg atctgctctt
    19801 tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata tctccatgta
    19861 cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt cgagcttctt
    19921 gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag tagaacagaa
    19981 cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca tcttcatttt
    20041 cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca tggttttgtt
    20101 gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat cgctcaacca
    20161 taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag gaaagtttat
    20221 cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga tacaaataag
    20281 agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga tcttgcatca
    20341 atccatggtt agcatattct ggataagttc caactccagt cacaccaccc attttataac
    20401 gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc agtttatttt
    20461 cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa cggataaaag
    20521 ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg aacacatagg
    20581 aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat tcaagatttt
    20641 ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata acagtatagc
    20701 aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa attactgctt
    20761 ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat ggatataaag
    20821 aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca tatcgaccat
    20881 gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa
    20941 atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat cacgattaaa
    21001 ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat ctttcacttg
    21061 attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt
    21121 gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac attggccaat
    21181 gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa aatcgaccta
    21241 cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct
    21301 agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat tctcgagcag
    21361 atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa agctttgatc
    21421 tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat cacggacggt
    21481 atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt tgatgttaca
    21541 gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt cggtgatgtc
    21601 ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga tacagtaacg
    21661 ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt
    21721 tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga agctttagaa
    21781 aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca agcattaggt
    21841 tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca agaacgcttg
    21901 gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta ccatgttaat
    21961 aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga acctgtaacg
    22021 attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa tgattttgtc
    22081 ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat ggattattta
    22141 gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt ctttgacttt
    22201 ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga aatgtctggt
    22261 tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa agtcgatgca
    22321 ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa
    22381 ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc tattggacaa
    22441 aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat
    22501 ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg
    22561 atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt tctgaaaatg
    22621 gatgagcaag atagtgtgaa ataacttata atcaatcgaa aaacctatta aataaacctt
    22681 tttgagtaga tgcaacactg aggattatct gtataaaaga ttttaaactg tcatttggtt
    22741 atcacggcga tactaaaatt aacaaaattg gaatattttt cttgaagatt tttagctgtc
    22801 agtatatgat gaaagcatct cataggaggt gcttttttca tggttgataa ttcaagtgga
    22861 agggttctta aatctctaag aaaagagaaa aaactatctc aaaaaaagtt agctgattta
    22921 gcaggtattt cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa aatccctaaa
    22981 gacgtagata atactctaag caagattctg aatatagaaa ctctaataaa agatgaggaa
    23041 gataagattg agattctcat cggtaaacta attgcataca gagatatgaa taagttactt
    23101 aacaaagagt tagctgataa tataggaatt agtgaagtat tactcagtta tgtgttaaat
    23161 agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct attaagtaat
    23221 gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga tatttagttt atcaaaagac
    23281 gataaaattg ttatgggaaa aagaatacga gaagtccgaa agaaccggga agagacgctt
    23341 gaaaaatttg gtaaaaactt taccatctat acaggcaaaa atgttataag tagatgggaa
    23401 aaaggtatta atattccaga tattgaaaaa ctaatgaata ttgcctatct tggtaaggtc
    23461 actgttcctt accttatgta cggagaagat tataagaata tacttccaaa agatgaaaga
    23521 gttagcgatt ttaaaaagat aaattctttt agtatgggat taaggatgag gaaaattagg
    23581 aaagattatt atctagaaag ggaggagttt ggaaagcttt tttctccttc aatttcaaaa
    23641 tggtccatag ataggtatga gaatggtaga gatataccca atactaatcg aattatccaa
    23701 tatgcttata taggaaatct ttctttagaa tttttgattt acgggatata aaaatagttt
    23761 acgtcgatac ttattaagtt atcgacgttt tttattatag aaaaaattta taaaaggagg
    23821 tttcttgata tgataagaaa caaaaagggt ttgttgaaag ttgctttact ggcagctttt
    23881 ttctaagtaa ctttttgaca agctcaatgg caacacaagc ggaagaagta atacaagaac
    23941 taactgttat gaataacagc tagataaaat gggctatgta tgttatccaa caatttagta
    24001 atcactagaa gcctagcgaa agttcgcttg ataagttata gatgagtatt gatttgtagt
    24061 gtttccaaat caaaagggta cagagccaca tgaaatatgt tgtcctctgc agcgtcagga
    24121 tgggaatcca taccatggag attcggagat ctgtgaggga atacttacgt ggttaatatg
    24181 tcagttgtgt tggacgtagt aatggtcata gtgggtagat tgactgaatt cttatatgag
    24241 aaaatgaaac caacaagaag atagggttat ttatacagaa ttggggatct tttctggaaa
    24301 agtaatcgga taatgcgaga ttcgtacggt cttttttaaa tttctctaaa aaaaacggca
    24361 tcgaatgggt tgggtgattg ataagcaatg aatcatagac tgatatagaa taaaaaatga
    24421 ttctatttat ctttttacat ctatcgtttt ttcaagtagc cgcaaattat atcagcattc
    24481 aacaattttt gtatacaagt tctagtaaaa ggatctgaag aaaaagttgg ttgttgtgaa
    24541 gataaaaaaa gaatcaggtt ttactgagtt tttcttcaaa atttgtgtta ttaggttagg
    24601 acaaagataa gaagaagtat gaagatttaa aaaaggaatg aggaaaagat agtaatttcc
    24661 gattattgga gtttttttat ttataaagaa gaagcgaata agttgtgatt cctagatttc
    24721 gcgcgtgatt aattaatgag caaaaaatgc ggaaagtttt agtgtttaat acacgaggac
    24781 aaagtttctt aggtattgaa ggcacacgtg taacggttga tgggtggtgt gattgtttcg
    24841 aggttggaaa acatgttgaa cttatggcat ttactgagct aaaagataag aacaacgtgg
    24901 aatttttcga gagtgatatt ggttgggacg atcatgaaga agtttatgga aaatttattt
    24961 tgcggaaggg aaattcttat acgaatgggg aagcataagt agcgatttgt tagagatcat
    25021 tgataggatt gaaattgtcg ggaatattta tgagaatcta gagttatttg aggtgataga
    25081 atgagtgatg gttttgaaca agtatcaata attaaaaatc aagttcggga gattcttaga
    25141 aaaaaaggct atttggtaga tagttatttt gaaggagatt acgaaacatg ggtaggtgtc
    25201 tatgctcgtc cagagaataa acctacctat ttagatccta cgacgtcaga agatgcctat
    25261 ttacaaaata ggtatcgagt agatggtttt aagcaagact ttgcggagtg gtttgaatgg
    25321 gaaattgaaa acggagaagt aaaagaagaa taagagtact tgttgtatca aaataagcca
    25381 gtagaacaac caaaccagta gctagtaatg acaataatac tgaggaatcg gatcattatg
    25441 gtatgattga tatttctctt ccagaagatc ttaagccaga tcctgtaaca attggcaata
    25501 gtgtcatgat ttttgattca ttagatgaag ctatgtcata tggttggaaa gaaattaagg
    25561 atgaaggtag taattggtat ggcatgagat ttggtgatgg tgaagttgcc aaaaaagctg
    25621 gtattccaat tgggaaatgg acagttgatt tccacgaagc aggttctttt tttgttctgt
    25681 aattatagtg ggagggtaaa atcattgatg aaatgtgttc agataagagc gccagataaa
    25741 aatagaagag acagttcacg tcgttatcac ttttggtaac ggcgttttgt tttattatat
    25801 aaaattctca ggaaaaggag gaattcttgt gtatgcaaaa acgaaaatga attggttgaa
    25861 agcactcgca ttggtggctt tttctcaatt ctgtttcttg gtgggaactg ctcaagcaca
    25921 tgcagaagaa acgacggcta tacaacctac gatggaagcc attgcccaag tcgcttctga
    25981 agtgacagaa gtccaaccag cagaagccgg ggtcccagct ggtgaagcca ctgttgaaca
    26041 ggcccctgtg acagaagcgc caacggtcaa ttccaaaacg actctcgatc agacgattgg
    26101 ggaaacacaa cacccaggga atagtcctgg tacttcacaa ccagttgaag aaactgcctc
    26161 aattacaacc cctgcttcag aaacgtccac tctagaagga gaaaaagctg ccgaagcaac
    26221 gccttctgtg cagccagagt cttctgagaa acaagcggaa gaaaagaaac gaatagaaga
    26281 aacagtggaa caaccagcgg ctactgctga caaagaagga aaaaatgagg aaaacgttga
    26341 aaaaacaatc tcttcaaccg cagctagtca aaatcaacca acgaaaccaa acaaagaatc
    26401 tacggctagt attgaaaatc aaaagaaaaa agagcagaac attccaaaaa caggtacacc
    26461 tacactgtta gtaacagtac caaccaatcg ttcggttcgt gttcgccgtg caaccaatgc
    26521 agctgtttta ggtggtcgga aaattgattg gggcgaaaat attggtgtta cttctagtcc
    26581 gaacaatcta aacttcactt taaattatat ggttcgtttt gagattaatg gacaagtggc
    26641 gttttgtatt gagcctggtg tctcagccgc agaaggtcaa gtttatacag ctactgcttt
    26701 gaaaaactat ttgaaaaatt caaatgtacg gaaaaaaaca agcttgatct cttatttagg
    26761 atatgtcaca aataaagaca aatccgatga acaatacatt gcggcacaat ttatgatttg
    26821 ggaagctttg ggcacaaaaa tcccaatcaa aaatatccgt gttgattatg atcgtcgaaa
    26881 agcagaaatt caacgtttga ttaataactt tggtgaaaaa gcaactttta ataatcaaac
    26941 acatacggta aaaattggcg aaacgttgaa agtaaagaat aaaaatgatt ttttgaaata
    27001 tgtgaaagac attcaaaaac caaaaggtgt caatgtatct attcacggca atgaagtatg
    27061 gattacagtc acaaaagagg caccggataa agcaaaaatt caattgaata aaattaaagt
    27121 tcaaggtgcg ccattagcct atcgtcgtcc aggagctcaa acaattggtg tgttacatcc
    27181 aacagaacct aatgcgtctg ttattaattt acaaattcta aaacaaggaa acattcgtgt
    27241 gtttaaagaa gatgcggaaa ctggtgtgaa agcacaaggt gcagcttctt tagaaaatgc
    27301 ggtctatggt ttgtatcaag cagatgggaa aaagattaaa gaaattacct tgaaaaaaat
    27361 taacggcaaa gtacaggcag aaattaaaga tcttgatcct ggcaaatatg ttttaaaaga
    27421 aatcaaagcg ccaaaaggtt atgtgctttc tgatgaagca attgaaattg ttgttgaacc
    27481 tggaaaaact gtcaatgcga ccgcaaaaga tcaagtgatc aaaggaacca ttgatttagt
    27541 gaaagttgct aataaagatt tagtcgattc aacgaatcca gataacaaac caaaattagc
    27601 tggcattgaa atcactttga cgtcaaaaac aactggcaaa gtggtgaaaa ccgttgtgac
    27661 agacaatgat ggttatgcta cttttggtaa aaataccgtt gtctttgata cgtacattct
    27721 ttctgaaacc aaaggaaaag aagggtataa actatttgaa ccacttgaag tcacaatttc
    27781 tgaaaatggg caaactttcc attatgtttt agaagataaa gtaattgaac aacgtcttaa
    27841 agtggtgaaa gtggatcaag aaacaggaaa agtgattcca cttgctaata cagaattcaa
    27901 gatttttgat actttagcca atcgttatgt cacaatggag attccaaatg atacagaaac
    27961 aacggatatc tttaaaacca atgaaaaagg ctttttccta actaacggta cacttactta
    28021 tggaaaagat cgctatcgtt tggaagaaat caaagccccc gtaaattacg ttttgaatca
    28081 aaacccatta gtgttctcga ttaccaatga tacagaaccg atcaaagtga tccattttgc
    28141 gaaccgtcaa gcacggaaaa gtgtaaaatt attcaagtat gaagaatgga acaagaagaa
    28201 aacgccgctc gcaggtgtga actttacctt gtataacggt aaaaatcaag caattggtga
    28261 atataccact gatcaaaacg gggaaatttt agtgaaaggt ctagttgccg atgactatta
    28321 cttcttggaa aatgccccat tagaaagttt ccaaccaaat acagataaac aagcattcac
    28381 ggttgcattc aaacaaaatg cgccagatga tggggaaacg ttagtggttt ctgtacgtaa
    28441 ttacttgatt ccaccaaaac tagaaacaca agcgtcaaat aaagccgatg gtgaaaaagt
    28501 ggttgatcca ttagaaacga tcgtcattaa agacagtgtt tcttatacca acttgtttgt
    28561 cggaaaagaa tacaccattt caggagtatt gatgaacaag gcaacgaatg cgccaatttt
    28621 acaagatggc aaagaaatca caagtacctt ggcctttatt gcagacaaag tcaatggcac
    28681 aaaagacctt gaatttgtgg ttaatgctag tgttctgaaa ggtcaaacga tcgttgtttt
    28741 tgaaaaactt ttccgtgatg gcattgaagt tgcagcacat gcggatatca acgatgaagc
    28801 ccaaagtgtg aaagtgaata atccaaccgt tcataccacg gctgtctttg aagacggatt
    28861 gacagtcgca gacccaacag gattgtttac cttaaacgat accgttgaaa ttaccgagat
    28921 tcttccagga aaagcatact tggtcaaagg gatcttaatg gataaagcaa ccggtgaacc
    28981 cttgcttgtg catggaaata cggtagaaac gagtgttggc tttattgcca aagcagaaaa
    29041 tgaaacggtg atcgtaccat ttacctttga tttgacaggt ataaatggtc gtgaaatcgt
    29101 cgtgtttgaa agtcttttta gaaatgatga agaactagca aaccataaag acatcaacga
    29161 caaagggcaa acggtacgag taactaaccc aagtgtacgt acaacggcta cacataaacc
    29221 cactggcttg aaagaggtta atccattaac gaaagtcacc attacagacg ctgtgaaata
    29281 ccacgatttg gtcgttggaa aaacatacac agtcaacggt gttctaatgg acaaagtaac
    29341 ggaaaaacca gtgctagtag atggaaaaga agtcacaagt accttaacgt ttgtggcaga
    29401 aacaacggat ggtacagtca atctagactt tattctagac gcacgtgcgt tgcgaggcaa
    29461 agaagtcgtg gtctttgaag atctgtttag agaaggcgta ttattggctt ctcacgcaga
    29521 tattaccgac aatgaccaaa ccatcaaaat cagtaatcca accattacga cgaaagcgac
    29581 gaataaaaat ggcggaaaag agatcttggc cttgccaaat caaactgtgc ttgaatgggt
    29641 caaagtagac ggtcttgcga ttggtcaaat ctatcgctta attgttcaag gctacttaac
    29701 accagacggc acaccatttg aaggcactca tgcggaaaaa gtctttgcgg ccgataaaga
    29761 aatgatggaa ttcttatttg aattcttagt ggacggtcgc aatcttgcag gaaaaggctt
    29821 gagttttgcg gaatggctcc aagcgaaaac ggaagatgag aaagaaacgt ttgaagaagt
    29881 ggcgaaacac aatgtagatc taacaaacaa agatcaaacg gttcaaattg tggaagcgcc
    29941 aaaaccaccg caaccagtga aaccagaaca acgggttaaa ccagctactg ttgtaccaat
    30001 gttgccaaaa acaggtgaac aaacaagtag tatacttatg gttcttggat tagtagtatt
    30061 agtcattgct ttaggtataa cactttataa agtacgtaaa gccaaagatt agtcatgagg
    30121 aacaagcggt taggattttt aaccgcttgt ttgattgtaa tgtagaaata tttagaattt
    30181 attttggttt ttaagaggag gaaaaacttt atggcagaaa aagcattgcg tatgaagaaa
    30241 gagtttttag gtcaagtatt tttattgggt tcacgggaac ctaatccacc aaaggaaatt
    30301 aatggtgtgt ctgggcgata tgttcaatta caagcagagt gttcgcgaga agaacagcct
    30361 gctttttttg tttatctcac agctgaagag gataaaagat tacgattacc agaaaaagag
    30421 gtgcgtattt catttgaaga agcttatttt gtttttcgta aagaacgagt gaatagtcgt
    30481 gaaacaaatg atatgggaca aacagagcgt cgaacaactg gctatcaaga tgttggtgtc
    30541 tttttaactg gtgtaaaaat tggggaggtt ttgtaagatg ggattaaaat ttccaaataa
    30601 ttatgacatt acagaagcat tagatgaaac ttgtttagga gccgtacgct ttggcgcatt
    30661 agaagagtct gatcgtgtat ttgataatga aacaggcgat tatacgggag aaattcgatc
    30721 acagcgagca caattagttt ttggaaatcc agatgaacca aagattgcac gacgagttaa
    30781 atttccagca gatgtagatt tgactggttt taaacgacgt gatcctgtga aattaattga
    30841 tccagaatat catgtggtat atattccagc acaagatggc aatcgtgaat attcagattt
    30901 agtgatttat gcgaaaggat tagagaaagt agaagctcca acaaataatc ctaatatgaa
    30961 acaatcacaa gaaaaaggtt tagatcaaaa acaaccaagt gttgattcta aggaaaaaga
    31021 gaaaaaataa ggtttttttg aaagaatagg aaggagtctt cctattcttt ttatctaaga
    31081 tagaaggaag gggagatttt gaattcagat aaaaccaatc aaatactttg agagattgtg
    31141 cttgttatca aaatatccaa caattgattg agtattttgg agacaatcca acgatttttg
    31201 tacaaacaaa tgcatatatt tgtgcggtta ttgggcagtg tatgttttta cgtgatcttg
    31261 ttcgtttttc attaccgtac cagtggaaac aagatcatca ggaagtgatt tggattcgta
    31321 tagtacgaat atgtaatatg tgtgcacacc cttataaaaa tcgattaaat ctttttcgat
    31381 tatgtttttt tactaaatgt gatattcctg tattagttgc tttttgtcaa aagtttttag
    31441 cacaggaaaa gtaaaaacag aaaggaggaa tgtgacaatt gaaagctcat tttttcaagt
    31501 atcgtggtcg gcgaattcgc tatttcaatc gttatttatg gttttgctat tatggtttct
    31561 ttttgtttcc atttttgatt ctgttaggtt ggtttagttg gaaagtattt tatccacgtt
    31621 taagtacgtt ttcgaattgg caaatgtatc ttattcctgg aataagtatt gtgttgtatt
    31681 tgttgctggt tagtgggtta attttagggt tgatgcattg gtcgcgacta aaagaaggat
    31741 attttgcctc tgtttattgg cgtcagttac ttgttcgaat gttaattgac aatcggtttg
    31801 tttatacaaa gaaacaagtt agtgagaaac aaacaaggga aaaaatgaga ttaccaaaag
    31861 tttattttta tcaaaagaaa caagaattag tggtttcttt tcctttagat ggtgggcgtt
    31921 ttcacgaacg atttttaaaa atgggtcatt tgttatctga agtatttttg cgtgatttaa
    31981 ttcgtgaaga acgagaaaaa gcccgaatca attatgtatt cctttcagat tctggtcgga
    32041 ttcctattga tcaatgtata gcagaaaatg gacgtattca tttaataaaa actttggatt
    32101 gggtatatga tcaatcacca aatatgttga ttgctggtgc aatcggtggt gggaaaacct
    32161 atttattgta ttctttaatt caagcttgtt taagtgttgg gacagtagat atttgcgatg
    32221 gaaaagcggc agatttagca gcattaggtg acgtcgatgt attcaaagga catgtgtttt
    32281 acaaaataaa tgaagaaatg attatttgtt tacgaaatgc attaaaagaa atgaatcaac
    32341 ggtatgccta tatgaaaacc atgaaagatc ctaaatatga accagggaaa aattatgctt
    32401 attatgggtt accaccgcat tttatctttt ttgatgaatg ggcagctttt tatggttcgt
    32461 tggatatgga agcgagaaat agagtcgata aattgattca gccattagta ttaaagggtc
    32521 gtcaagcagg gatgtacatt attttagcaa tgcaacgacc agatgcagaa tattttccaa
    32581 gtggtgttcg ggataattta acattccgta tttctgttgg tcgattgtct cccattggtt
    32641 atttaatgac ttttggagaa gattttaaaa ataagccatt ttacaacaaa gatcaacctg
    32701 gtcgtggata tgcttttcca ggatttgatg tacctgtaga gttttttgcg ccatttgttt
    32761 acaaagatta tcactttatc aaagaatttg agaaatttac tgaaatgatt gtctatgatt
    32821 ttacagtaaa agaggatagt caagatcaaa cgatgggaga tgataatcag ttgttacaag
    32881 aacaactaga gcgccaaatg tatttagcaa aagatccaaa aggaacaagt ttttctgatg
    32941 cgcatatttt gtaatcaact aaggaggtga agttaaatgg cagagagttg ttattataga
    33001 gtagctacag caatacgaat gattaatcct tctctttctt ccagaacgtt ttatgattgg
    33061 ttgaatcgta ttgaacaagt aactgattac cgctttttgc gcaaagaacg tgtttttaca
    33121 ggaaaagtta ttaatcaagt attgctaacg aaaaaagata tagaacgatt gactcgattg
    33181 tatcattatc gagtagattt agaagaggat ttaactttgt caatttatcg cgttttttct
    33241 cctgaaaaat atagtgaaat aacaaaatta gatcatttaa ttttgtagtt attatctatc
    33301 gggagggctc tagacgtagt ctggtagccg cactctgcct ggcggtgcgc attaaggctg
    33361 cgaagcatgg gcctataatg cgcactcgct agcagcgtgt gcagctcaca aagaggtcgt
    33421 gcgcttgcgc acggcctctt tgtgtaaagc cccccgatat ataatatggg gggcagaaat
    33481 aacataacac atagaaaaaa gttttataaa cccttttgta tcaagtgatt atctaaggtc
    33541 agcagttgta ctacttttcc caaaaaacga gggggactag ttgatatatc ggtgtttcta
    33601 aagggttaaa aaaaatcgat tttgttattt tataaaagga gcgagaaaat gacaccgatt
    33661 gagctaaaaa gctacagaaa gaagttaagt gcaattcata atataagtgg agataaaaag
    33721 ccgctatcac aagcaagatt tgcgcaattg atgggctgct caaaagatta cattagaaag
    33781 atggaacaag ggaaaagaaa aatcccagag gattttgaac aacgaatcaa ggaacgcttt
    33841 cgattgtata tgaaaagcgg aatagatctt cgagcaatga ttgattattt gcgtctttcc
    33901 ttttttggga cagactacaa aacagtgatt caacgtgtat tacgaatgga tctgacagaa
    33961 tttgaaacac gtccaacggg cttgtatctt tttgactgtc tttctgttcg tgggaatatc
    34021 tgggtgttct ggcattcaaa acgaactgta acagaaaatg tacttgtgca attttcagga
    34081 caaggttgcc gggaatatga ggaagtcatg cgtgaacaac aaacagattg gcgaacatgg
    34141 ctatattcca tttggaaaaa tcaaggaaag ttggaagctg gttatacacg tgtgcaatgt
    34201 agccgattcg atattgcgtt agatgaactt tggggattgg aaacggaaga acatttcgat
    34261 ctctttgaat tactagaaaa acaaaaagca ggtttattag aaatggactt taagagattc
    34321 cagaatattg gcggttgttt tttagatgat ggctattttc gaagtgaagg attatctttg
    34381 tattttggat cgcgaaagag tcctctcttt tttaattttt atgagaaacg ttttgaaatt
    34441 gcgaaccgag aaaaaatttc agaattagaa gcattaacga aatatggtat ttataatcga
    34501 tatgagttac ggttagccaa tgagaaagca acacaagcag tagaagcatt tattttaggt
    34561 gattcaccga aacgtttagg agagattggc gttggtttaa ttaatgcaaa acttattgtt
    34621 tgggatagac tggaaagtga tgcacggatt gttgataaac gatgggcaaa attatttcgt
    34681 tcgatggaag aattggattt tacagtaaag ccagaaaaat tctcattaga tcgttcagaa
    34741 cgctggtttt tgaaacaagt agctccgaca ttaaagttgt tacaagaagc agatcgtatt
    34801 ttaggaagag actttgtaca agaagggatt gataatgccg aactaaatga cgagcaacaa
    34861 aactatctag aatggatggg aacgtcagga gggaaaattg catgaatgca tatacgacaa
    34921 cagaatggct gcgcctgttg gatttaaagg ctgcaatcga agcattaaat gaaaagatgg
    34981 ttgatttgtc ttattttcgt tttcgagttc cttatattga acaagcggta aaagctggac
    35041 gttatcagga aaaagagaac tggcaagaaa ttgcacgctt attagaagtt cgtaagggat
    35101 acgaacaaga actcgaagag ttggaattta gccgaaggaa aggaaggcta gaattcattc
    35161 gcttttatcg attttcattg ccaatacctg cgattttagc ggttaaaaaa ggctgcgata
    35221 aaatgaagat atatgaaaac tgtgtggcag cacttagcag cgaaaaacct cttatggaag
    35281 aaatttcatt ggtgactgtc acttgggaaa tgagtcgcca gccaacagaa gaacagacat
    35341 atctttcttt agaagagatt gagatagaat tagaggaaat tggtcgctat gccacttgtt
    35401 ctacctattg tggatcggtc attagtattg ctggtgtgat tgtctaaatg aaaaaaatta
    35461 tgatttatgt tggtgcgtat tggtcacgtg atccaacagt attagaaaat ccagaggcgg
    35521 tacactattg cttgcgccaa ttattctatc tgtataaaga acgattagaa tcgttgattc
    35581 gtcagttacc atatactgac agacgtttag atgaacttct tctaaggtat ccagcaatgt
    35641 ataaacgacg aaaaaataga ttgttaccag aagagtatcc cattgaaaaa catgaacttg
    35701 aagggcgatt tgtggcttat ttctatgatg atgttcgtat gcgcttagta gaacaacgaa
    35761 tggaaataga aaatgatcgc tactatttta tcaattattg taagaagcgt aagtatcaag
    35821 taactgatga tttttatgat tgtattttac aagatgggga agtgattcta tccaaaattg
    35881 ctccaagttt tcgtgaactt gtgccgattg attttttgaa aaaatgtcat ttgcggattc
    35941 ttccgtaaaa aagagctata aaaggaaaga aaagtagaag ccaaatcggt ttctgctttt
    36001 ttattttttt taggaaggag aagatggtat gtcactggaa tttttaggag agccaattga
    36061 aaaggaagag ttcgtttcgc attatatgga tttgtttatt caggtgataa aaaactttgt
    36121 gcgattgatg aattttatcc acgcgaacaa gactatctag ccgctgaacg aataaaaatc
    36181 agtttattat gggaaaaatt cacagatgaa aataaccgac cgccagatta tcgctattta
    36241 tcagattgtt taacgaataa tgtgctttct aaagaatacc tatttcacaa acgtgagaaa
    36301 aatcaaatgt cggttgaaca tttttgtgat tgttatattg cattattgcg tagtcaacag
    36361 ttattaacag tagaacgata tggtgcagag tttggttatg tcatgcaagc tgagcgtcat
    36421 agagctacaa gatgctatga aaaagaagat caagtgattg atggctatga agaattatgt
    36481 attcgaaaca atcgctattt gcaacaacgt ttatatgaac aattgatgat ggatttttca
    36541 aatctctata gagggtatga atgttcgatg attcaagaaa ggtagaagaa aagggggaat
    36601 ttttatgtgg cgattattat ttttggtggt atgtggtatg gcattgttct atgtgtatcg
    36661 tgtctatctc tatccacgaa tggaacgatg gacaagttgg tatagagatc ctttaaaaca
    36721 acgaaaagga ttgaagcggt atcgttatga attacttgtt gaattttcgg atgggcgaca
    36781 agcacagtat tatttttttg caaactatcc aaaatacaac accagtgaac tattgattca
    36841 attattcaaa aataatccat atgttgggtt agaagaaaac ggacgggagt tcttttatcg
    36901 ttcagataat attagtgcgg cgtctatgaa caaaacacga attgcataaa aaattggagg
    36961 aataatcatg aaaaaaacaa cattagcagt cgtaacaaca ctaggaattt cattattttc
    37021 gtttcaatta gggagttcat tggtttatgc agatgaaact gtagaaacac ctaagtcaga
    37081 aattgtagaa acattgccag gggaaggatt accagatgtt gagacagagc taaatggagg
    37141 aacaacggaa aaaccagcag aacctgaaca attgcagtca gagaaacctg aaaaagagcc
    37201 agtgacacca gaaccagagt ttcctgaatc aactttaccg actgattcga tcgaagaaga
    37261 aggtgtgcat agttcaacaa caacggattc aagtatagtc acaactccgg aacaatcagg
    37321 aacaaccagt tcttctgaaa agaatactga ttcgtcaagt acggaacagc caacgatgtc
    37381 gtctaccagt gaagaacaaa aagaagaacc gaaacaacct gttgaaaaat cagaagcaat
    37441 tccaaaagaa aataaaccta ttgttccagc aaaagaagtc acagtatcgg taaccccaag
    37501 cggtgaaatt acgacgaata cttctcaagg aatgagtgta ccgattgtca ctagtaatgt
    37561 agaggaattg acccatattc caacaccaac caccccatta aaagtagcag gtgatcaaac
    37621 aattgttggt gtaaaagatg gtattccatt aatccaagat agtgaaggga atttggtaaa
    37681 ggatttgtcg attcctgtaa agaaacttcc aagtggcaat attgaaatca aaacagcaga
    37741 tggaaaaaca aaagtcttac caaagactgg tgaagaaata catgttgcac tttctgttct
    37801 aggaggagtg ttgacaagta ttgctggatt tgttggatat aaaagaaaga gaaaaggaat
    37861 agaaaatgag ggtaattgaa tggactattt ttcagaaaaa caagcttatc gttgtcaatc
    37921 gtacgtacta gtaaaaacat actagaagga gtagcttatg aaaaaacgac aactacggcg
    37981 gaaagaaaaa gtttatacag agaaagtacc gaagcaaaag aaaatccatt caaaaaaagc
    38041 agtgaaatgg atttggtgtt tggttttatt tctttgtttt tcagggggct tggcattttt
    38101 tcaatccatg aaaacgaagg ttcaagtgtc gtttttgaaa gatgaaatcc aacaattaca
    38161 aaagaaagaa gtacaaagtc ggtcagaatt aacgtacaca cctgaaattg aatcctttat
    38221 gaatcgtttt gttgctttgt atatgaatac attcgataat catgaacaag aacaacaacg
    38281 tcaagaaaca ttaaaaaaag agttttatgc acaaaacttt attcaagaac caggaagtgt
    38341 ttcaggtgtt cgaaaattaa ctgctgcttc gtttattgga ttaaaagaaa ttgaaaccat
    38401 tccaactgct agttataaag taacttatga tattcgctcg atgaatccag agagtaagaa
    38461 agaaacaagt gtgacaatca cacaagtact caatcttccc ttttctattt caaacaaggg
    38521 ctgtcgtatt attgcttatc cttattttac ggcactacca aagaatacag aaaaacaacg
    38581 aatgattact tatgatgaca cattatacga aacggttgat gcgtcggaag cgaaagatgt
    38641 tgaaggattt gtggaagatt tcttagataa gtatgcgaaa aacaatgcaa aagatatgtc
    38701 atacatgatg aaagaacctg aaggactcaa tcaaacagct aaagttgatc aagtagagat
    38761 gaaagtattg cgttataaag aaacgaaaaa accttttatt gtaaaagcaa cggtcgtctt
    38821 tcgagatggt gatacgtcac ttttacaaaa agaacaaatg acgattgtca ttacgaaaaa
    38881 aacaaatcag tattatgttg aaaaattaac acataattgg ttggatagga aaggagaaga
    38941 acggaaatga cactgaaggg aattgtagat tttatattaa cgcaaggtgg ctatttttta
    39001 ataatggtgg ttgtttatct gggattgcga aattttatga aatcaaaagt aggaaatgca
    39061 ttaatatcta tcgcagcagg tgccgcagtt tatttcttct taaatgatcc aacacgtgta
    39121 tttgatgcgt ttggtaaaat cattgaaaaa ttatttatgg attaggagga tagtgaagtg
    39181 gattgggaac cttatgatta ttcgattgtt tatgcggaac cttatcgcgt acaacagatt
    39241 acaagcaatt ggcgtcttcc atttccagtt gcattagtca atgcagcaac ttttttgttg
    39301 gtcgtaatct ttgaatattt atttttacgt tcatttattg gttgggtcat ttcttttttt
    39361 ccaccagcag gagttgtttt ttatatagga ctcccctatt tatgtgtgtt agcactggca
    39421 aaactaaaac ctgatggaaa aaaagtgcat ttttatttat gggattggtt ttgttatatg
    39481 attcgtatga tgaatggtga ttgtgtgtat tataactggg acaaggtatc aaacaatgca
    39541 ttggaagaat tactttttgt gaataagtat gtttatttag aggattagaa aaagggacaa
    39601 tcaactggtt gttccttttt tattgggagg tgaaattcat tggtgcttgt aaaagaacca
    39661 gtaaaagcat tcaagaaaaa ttgtttgtta aatggagagg attgtatctg ggcctactat
    39721 catgtttttc cacgaacaat ttcaaaaaac acaccaaaaa agacacagga aataaaagaa
    39781 atgatggctc gtttactgtt gcttttagca gattatgaag atgtggagtt ggtgatgtat
    39841 ccacgaacaa tgaatttacg gacgaagttt gacacattag gaaagggatt ttctcccgat
    39901 agtcaagcaa ttggtgcgta ttatgcaaaa gaaacagtga cattgttgga acgagaaggt
    39961 caagtctatc gtccttcatt tattttagga atcaaagtca aagaaaaagc aacggtggag
    40021 agttttcatg atgtaataaa tctaatctgg ttagatttag caggaagttt agcacgctta
    40081 tttggattag aagtagatga tttggaaact ttgttttctg aaagtgatcc ttatgtaaaa
    40141 gaagtgacac aattacttta tagcttttct attcgtccct tagtaaaaaa agagctgttg
    40201 tatctcaatc gtctgcaata tattcgagga atggctcaca aagtagaaga acaaaataat
    40261 tgggaagtac tgaaagacaa tcaaggaacg tatgtacgta taccgaagga acataaaggc
    40321 cttcttcaat tagaagatga gttagggagt agttatttag cattgttgcc tattggggaa
    40381 acacctgaaa atatgagaaa ttgtgatcta tttcaatttg ctcaggatct ttctttccca
    40441 gcagaattgc gaatgaaaat tcactatcca cgcttgaaag gacagggagg tattggttta
    40501 aaactgaatt ggctatcgga acggttcaaa acagagcata aggaattggt tcaaaatgat
    40561 gaagaaccta gtgaacgctt attaactgtt cgaggattag taaaacattt aaagcaacga
    40621 gtagataatg ggcaacctgt cgtcgattgg atcgcatgtt ttattgtata cggtcaaacg
    40681 gttgaagaag ttcgtcaaaa tagtgcaacg gttattcaac aacttcatac gattatccat
    40741 atccaccgtg caaaaaatga tcaacgagaa ttgttctata aatgtttatt agggcaacca
    40801 ttgggacggc agttacattg gaagcaccga acaactgtag aagcagttgg cgaatggttg
    40861 ttttcagcag tatcagaatt aggtatggaa tccggctggt atattggtcg gcaagataca
    40921 ttaggggatg gtggttctcg aggagaaaaa acaccattag aagaattaat ttatgcgtca
    40981 aatcgttttg tctttttgaa tcttctagcc attgcggaag gaatcaaagg tgcgttgttt
    41041 gatgcgcctc atgtagcagt aactggaaaa accggtaaag ggaagacctt tttagttggc
    41101 ttgctctttt tgtatagttc gtttatgaat gtacaaagtt tatttgtgga tcccaaagga
    41161 gaaaagcgca aatggtttac gaaactcatt caagacccgt attatcagaa acattaccct
    41221 ttgtttgtag aacatttgca aaaaatgagt tatgtcacgt tagatgctac aaatccccgt
    41281 aattatggtg tattagatcc aattgtttgt ttatcccgag tagatgcgaa acaagtagcc
    41341 caggatatga ttgatgagtt aagtccgtta ggtaccaatc accttttaaa aggggcggta
    41401 ttaaagggaa tccaagaagt tttagttcaa aaagaagccg gacaacaagt tggcttgatg
    41461 catgtggtcg actatttaga acaaatggaa caaaaagagg ttcgagaata tggtgagttt
    41521 ttacgcttga cggtagaaga ttcgatttta cgtttaggat tttcttatgg agaaaacccg
    41581 ggattagact ttaatgccaa aacaacgatt ttagagatcc aagatttgaa attaccaaat
    41641 gacaatgtcc gaccagaact atatacagat gcggatcgaa agtctttgtg tttgatgatt
    41701 agtttaggtc gtttttgtga aatgtttgga aaacgagata gtagtaagaa aacagcgatt
    41761 tattttacag aagcatgggt atttaataat tcgaacgcag gacgtagtat tattgcagca
    41821 atggcacgtg tggggcgttc ccagatgaat caattagtgt tagatacaca atttattggt
    41881 gacttaggtt cagataaaga aaaagggaat tttggtgtcg tctttgcttt tgatgaagac
    41941 agtgagcgag aaaagatttt gaatcattta cgtttggaag taaatgaagc gaacttagct
    42001 gaaatgagcc acatgattaa aggacagtgt tggatgttag atccctatgg acgtgtagga
    42061 aaactcaatg tacacagttt gtttgaagaa atgaccgctg cattacaaac aacggaaaaa
    42121 acaagtcact ctcaagtaga agaacaggat acgttaatca tgcaatagaa atgaggtgaa
    42181 acaatggctt atataaagca taaggaaaag aaatggtgcg ttttgttttt tttaacgatt
    42241 ggattcggac tgctttttgt tggtggattg gtgtatgcgg cagaaaagca ggtggatcaa
    42301 tcgatctatg agattgggaa atttcatatt gatacctttt cttcttattt atctgatagt
    42361 gaattttcat taggcaaaat gttatttggg gcaaatggca cagattttct aaaaatgatg
    42421 acgaatatgt tctttagttt aagtaaattg atttggcaag gccttgattt tgtcattgag
    42481 aagctctatg aaggagctgc aatgaacgag ttaattcacc agtttttcac tttttcggat
    42541 actatttatt caaaaatata cgatcctatt ggtgcgggga ttgtcgtctt gtatgtgtta
    42601 tatctattta ctcttcgtat atttagaggg aaacattacg caaaaatggt gtttattcga
    42661 tttctcttcg ttgtcatttt ttcaatggtt tggtttggct atggtagtaa cgtacctagt
    42721 aaaggagaag cttttaccaa agggttaaat gatttgtccg tggaggtaga aggctttatt
    42781 ttttctgcta caaatagtct agatgaattg gcgacaaaag atgtcaatgg tgttttagtg
    42841 ccaacgacac aaaaggaagc cattcaaaaa gtgcgccaac tttattataa agcggctgtt
    42901 gttgatccgt atatattgtt aaattatggg acaacaaatt tagaagaatt gtatcaagca
    42961 gagattgaac cacaagaatt tctagccaag gaagcgtcaa gtgaagaatt atctgatatt
    43021 gagacaaaag tgaccactgc tgcaggagaa gatagaggga aaaagaaggc ttatcgtgtc
    43081 tatattcaac caactaaagc tgtttataaa gcagtcgttg gttctatggt gccattgctt
    43141 aatctaagta ttggattacc acttgtagtg attggcatga ttcgttttat ctttcaaatg
    43201 ttggtgttgt gtttattact tgtgattcct ttttcattac tcgcttcttt tgttccagta
    43261 tttgattata tgctcatgaa taccttaaag tcctttttgg gcttgatgtt tcaaaaatct
    43321 ttttacagtg tgattatttt ggtggcattt ttgatctata acatagtaga cgatttaatt
    43381 ccaatgaata ctgtttttgg ttttttagga aatatgtttg tcaaagcact cctatcaatt
    43441 ttactcattg taaaacggaa agatttatta aagaaactag ggttaggagc tgcagatcaa
    43501 actataaaca acacgaaaaa catagcaagt cattctttgt ctcaaatgaa acaactgcct
    43561 ggtcaactat caactgctgg acaaaatttg gcaatcaaag gtgcaggatt agcagggaat
    43621 gtgtatcgag caacgaatcc actcgcttct gcagcgatac atggatttca aaagtttcaa
    43681 caaaaacgta caccacaaga atcggtaaaa gggaggcaag tggttaatcc ctttgaacta
    43741 ggtgggcgtt caaatcaagc cttgccttat agtggtcgtc atgttcgtta tcatggggta
    43801 gagcctgaaa tgtcttctac caaatcgaaa acagagtgga attctggtct ttcaaatggt
    43861 ttgattccaa attcgtccat gaattcagta agaccaaaac aacaggtaca atcattggca
    43921 caattgagta agcaacaagg gaataatcaa agaaaaccat tgttaaataa aggtatcgct
    43981 tttgttggtc aatcgattcc gttgccttct ggaaaagtac ccgtgccaca attaacagct
    44041 gctggttctc tttcacaaca gattcgccaa gtgaaaaatc aggcgaaaaa tgggagttat
    44101 ccagcaatgc agcagttatt gaaccaatac tctgttccta attcgtcggt agccactcta
    44161 tcaggaaatc gttctgtgca acaaccgggg caacgttcgt ctgaaagtaa accgtcgatt
    44221 tccgcaccaa aatcgttaca acctgcattt tcttctttag ataagccaac agagcaatcg
    44281 gaaaaaatag aaaaggatcc taaaacactg ccgattttgg acgcaaaacc aggaactgat
    44341 tttactttac caaaaggacg aacagtccaa aaaccattat cgaagaaagg atgaggaaga
    44401 aatgaagaaa gggtgtctag gttgtcttct tttctttatt cttccctttt ttctattgcc
    44461 attctttttt tcaggagaac tttctgagca tggaggagaa gctacaggtc tagctgtcgc
    44521 ttttaatggg gaatataccg aaggattacc gattttttca gagattaaag gacgtggtcc
    44581 aatcacagat gaacatgcgc aagcagcagt tggtgcagct gtaaagtatc agttattacc
    44641 gagtgtgatt ctttctcaat taggctatga atcagcttat ggacagtcac aatctggaag
    44701 aaacgatacg aacttttttg ggattacctg gtttcctggt tgtccctacc cacaaggttc
    44761 tgcacgtgga attggtggtt ctgaaggtgg tttctatatg aagtttccga acttaaaagc
    44821 ttgttatgac tattatggct ttatggtggc gagccaaagc aactttaatc aatgtgtggg
    44881 caataaagat cctggaagtt gtttattgat tctaggtcgt ggaggctatg cggcagcagg
    44941 tattaccgaa gggagtccgt attatcgagg agcaatgagt atcataacgt cctataactt
    45001 aacggaatat gatacttttg cgatcaagca ttggaatagc ctaccaacaa aaaaaacttc
    45061 gcttattgca ggaaaaagta aaggcaatgc gaaggttttg gatcggatca ttggacgatc
    45121 cgtaaataat ggggaatgtt atggtggtac ggcatggtat gtatctcaat taggtggccc
    45181 acagttaatg ggttctggac acatgtttgc gatgttaata ggggaagatt ataactggac
    45241 aacctatggt tggcaagtaa tcatgaaccc aaaaccaagt gatttacaag caggagatgt
    45301 ggtgaattgg caagcaggtg gtgcattgtc tcctggaatc tatggtcaca ccggaattat
    45361 aacagatgtt tcatatggcg gacaacattt ctctagtgtg gaacagaatg ccgagcaagg
    45421 acggattatg gctcggtatc aacgcacgta caatcaaaca aaaattcgta gtctggttcg
    45481 taaggtgaaa taagaaaggc agtgactaga aacgtgaaaa aagggataat tttagtattt
    45541 agttggttat gtttgatagg tcttactggt tgtcattggt tttcttctga aaaacaaaaa
    45601 acacaattga gaacgacaga agaaacaatc gaagcagtaa aagaaacaag tggaactctt
    45661 caaagtactg aaagtaacag tcaaattggt tcattagaag acgagacagc gcaacaaatg
    45721 caaatagatg aaatgacaaa aaatttctta aaagattttg ctagaaaatg gagtaatttc
    45781 acagatgttt ataaacgcaa tcaagctgtg agggactata tgacagatcg ttgtattaaa
    45841 gacaatagta ttgatgtaga cccgcatgta caaatgaaaa caactgggaa actttaccag
    45901 gtgacacaag atatagagaa tccggatcaa tatttgttat ttggagaaga aacaatgaat
    45961 aaggttactc aatttgtatt acttcaattg aaagtagatc aagaagctca aaagattgat
    46021 gcgatcaaga tttattatgt acggagtgcg tattaaaata aaaaatgaac actcatattt
    46081 attatacacc gaagaaatag aaaaaaacag actgtttttt taagaaaaac tatgtcattc
    46141 tttacttgaa aaaagttgtt ataggacaga aaggataaaa caaaaacgga aaaaatatgt
    46201 cttatgaata actataagaa ctattctatt ttttaggaga tggatacgag atgaaaaaac
    46261 taattggcta tagtgtgatg tgtattggat ttgttggtgt gggattgttt gtatcgactg
    46321 aatctgcttc tgcggctgaa tggtataatc ctatctggta taatccgaac attgaatgtc
    46381 aaaaaggcta tccgtatgga ccgacaaatc tgaaaaatgg aaaatgtaga gtaaattgga
    46441 atggcgttgt agatagtgta attactggtt caatggactc tgctctacat ggctttacgg
    46501 gtcactagaa ggaggaagaa aatgtctgaa gagaaaaaag aaatcgttga atcattagta
    46561 gctttaaaag attctctttc taagattgaa tatgttgata ggcaagaaat tcaaaaacta
    46621 attgatgata ctattattga aatccaagat gcgcgttgtg aaggtataaa aatttctgta
    46681 gctttatcta aagtaataga aaaaatgaac cgttcattag cgtttaatgg attgaagcta
    46741 gatagacaaa ctagtctgat atgggatcat ttaaaagact tgtatgacaa gtctaaaaga
    46801 agtgaacgga ctgctgtatc aatcttaaaa ggcctttggg gaatgaattc ataaacaaga
    46861 tatttaaaaa gtaacttatt ttttatggag gagaaattat gaaaagaata attaaaatat
    46921 ttggtgtttt aggtgttttc ttattaggtt taagtacttt tgttagtact ggaacaagtg
    46981 tgtatgccac agaaaaagtg aatacagttg atttagaaat aaagaataaa ttagtagatg
    47041 aattaacttt ttattttgat acagttggtt ttttagacga acatggtgta taccatataa
    47101 aggattttgg tgctattcgt gaacgaatca gtcaagataa cgaacaagga agagcagcaa
    47161 gagtctttta tgaacatatt attgttccta gaatggagaa agttgattgg aaacgatatg
    47221 ctggatgtgt agccattaat tctcttcctg gaggaggagt ggcttgggct ataggtacgc
    47281 agggagattt tattgaagca ttaaaagctt ttaattttga aaaagctgcg gatttattat
    47341 taaaaattgc agaaaaacaa ttaggaaaag ctgcagctaa agagttagct aaggtaaata
    47401 ttgtagcagg aattgcagca gctgctgcta gctgtgcatt agagtaaaag tttatgaaaa
    47461 gtttagaaca agagcatatg gcttttaaac aagcaatgtt taaagaaaat atctatttaa
    47521 accataacta tattcgtgtt tctaaggcat gttcaccagt attgaatatg cttggaggtg
    47581 gaaatggatt atatcattta ctgtttgtcg atgtttgttg gttaattttt ttaccagatg
    47641 aactagttat agtcaatgaa aaaataacaa gtaaaaatga agtgtttaat tattctttaa
    47701 caagaattaa ttacaaagag attaccaagt tttcactaga aaaagttcct ttttggggag
    47761 aattttgttt aaaaattaaa tgtaactgga aaagaatgta tttctatatt gatggtgatg
    47821 atgctcttac attcggtaaa actacttttt ctagttttaa ttttcagttt ctattaaaaa
    47881 ataattttta tggtttattg aaataaggtt gcgaaggaag atgtagagtt atttgcactg
    47941 ttgtgttggt atatctctta atgctgtgtc tttttgggga gattaattag tggagtgatt
    48001 atatgagtat agatgattgt ttagaagaac aaataagatt atttttaaag tttttaaaag
    48061 ttaaagttca aaataactac ttcacatgtt atagttatgc acaccctgtt ttaaatagtc
    48121 ttccttttaa ttctttagtg caaggattga ttacaaatat tgttttatta gtctgtacac
    48181 caaatgaaat atttgtaaaa aagataggca gtggtatttc ttcagtaaga ttaaaagaga
    48241 aagaatatac tcaaaagatt attcatattc ctaagcctga aattaagcgt tttgagataa
    48301 aagactattc tgctttaggg ctgttctcaa ttggttactg gtgataaaaa ataagaatac
    48361 taaaactata cgtagaatat aagtaatcgg catcaatcct aattatgtga ttgaatgccg
    48421 atttttgtta cgcgctattt ttaattatct gtaagaagaa taattttatt ttactcatat
    48481 tcgttttcac tttgaagaag taaattgcaa tttcttataa gttaagcagc tatgcaaaag
    48541 gctatttatt ctattgtgaa atataagatg tagttgaaac aggatatata gaatcaaaat
    48601 acgaatctta ttttgatttt tctagttttt tgaaaaaagc accctttgat cgttgtatta
    48661 gtggaaaaga gaaaaaggag catacttatc atgtcacaaa actacaacta aatatctaaa
    48721 caacaccaat ttgatgccta ttgaaaaaaa gtattgcgaa atgaagccaa gtctattaga
    48781 aaatgaaata atagaataaa agaaaatggg aaaccgctga atgatgtaaa tgaaggtaag
    48841 tatccacgaa ctaattttga tgaacaagat atctattttt tatttgtatt ggagatacta
    48901 gtttaatatc aaaagttatc agcagtcata attagttatc caatgtaaaa agtacaaaca
    48961 atagttgtta tgcagaatag tttatgaaat atattacata ataagaaaac aagcgatatt
    49021 tttctgatca gaacaaccat ttaatttaaa aagagttaaa agttatatat ttttgaaata
    49081 ttttggatga gtagctaaaa caatattttg ctcattaagc attaaatgaa gatgcaacga
    49141 tgttagaaag ttagcttctt ctggatcttg ttttgttaat gcctcaaata gttgataatg
    49201 ttgttccata attttttccc actttaatcc ttccgttttt actcgtagcc atcgaaatcg
    49261 ttctaagtgt gtattgttat tttctaacca ttgccatact tcttctcgct ctgcaatttc
    49321 aaaacaagct ttatggaata aattatcatt atgaaaaaaa ctacgagcat ttttttgact
    49381 aacagctttt tcttgttctg aaattatagt ttctaaaatc cgtttttttt ggttattcat
    49441 tttggcacaa cactctaaca taatttgtcg ttccaaatgt tctctgacaa atcgagcatt
    49501 tttggccgaa tctaaatcaa ttaatgagat ataatttccg ctttgaggaa ttgtatcaac
    49561 aagaccttgt tgtcggagtt gaatcagtgc ttcacgtatt ggtgtacgac caatagaaag
    49621 tgtttcttct aatttttttt ctgatacttt ttttccaggt tctaagtcag catagataat
    49681 ttgtcggcga atagattggt aagcttgatt ttggagacta gttttaattg tcatacacaa
    49741 tgctccttaa cttttatttt aactgatatt ttaattctgt tcatgccata taatatcaaa
    49801 ttttgaagta atacactagt tataaaaata aaagaataca tgaaaaaaag aaaaaataag
    49861 tttcgataaa atcagtccaa aaagaaggag tattttattt ttctgaataa aaaatacaat
    49921 cattctgata tggtagaaaa aagcaagttt attccctttt tatattgaca acggttacaa
    49981 tgggttttaa aatataaaat gtatcaaaac taacatatca gtttgaggag gttaattatg
    50041 gtttcgatta aaaattggca aaattctatc gaagaattca atgaaatgca gattctactg
    50101 ccgtgttttg atgtcaaaga agttaagcaa gaaggccagc aacatccagt atggcttcat
    50161 tttggtggag gaaatcttta tcgaggattt catgcagaaa ttgctcaatg cttagcagat
    50221 caaaatgaat tagaaacagg agtagttgtg tgtgaaacat ttgatgaaga agtagtggaa
    50281 aaagcttatc atgactataa caatgacatt ttggaaattg ttatgagtgg agatggtgaa
    50341 ctcaaaaaaa gacttttggc agtaacggct gatagtttat attgcaatcc gaagaatata
    50401 gcggactatc aaagagtggt taaatatttt gaagatccta aattacagtt tacaacgttt
    50461 actattactg aaaaaggtta cagtttaaaa gattctaaag ggcaattctt ttcggtagtc
    50521 aaagctgata tcaaatctgg acctagcaat cctcaacata cgatgagtat tgtaacatcc
    50581 ttgttatata gcagatttaa tgctggagag ttgccgattg cgatggtttc tactgataat
    50641 ttctcacaga atggtcaacg ttttcaagat agtattttta cgattgcaga agcttggcaa
    50701 aaagaaggat atgtttctga aaaatttatt gcttatttaa aggataaaga aaaagtgact
    50761 tttccttgga gtatgattga tcgaattacg ccaaatcctt cagagaatgt aaaggaagat
    50821 ctattgatga tggggctcga ggatttagat atcattcaca caaaaaaaca taccaatatt
    50881 gctgctttca ctaatactga ggtagttcat tatttagtta ttgaagatag ttttccaaat
    50941 ggccggccta aattggaaaa ggctggtgtg attatgacag atagagagac ggttgataaa
    51001 gcagacatca tgaaggttac gacaagcttg aatccacttc atactgcttt ggcagtaaca
    51061 ggttgtctgc taggatatca atcaatttca gcagagatgc aggatgatga tttggttggt
    51121 ttaatcaaag gaataggtta tttagaaggt ttaccagtag ttgaaaatcc aggcattatt
    51181 gatccaaaac aatttattga tgaagtaatt aataaacgtt tgcctaatcc ctatattcca
    51241 gatacaccac aacgaattgc aacagatact tctcaaaaag tagcgattcg ctatggtgaa
    51301 acaattaaaa agtatgtcga aaaagacgga aatgcagaaa atctaacatt tattcctatc
    51361 gctattgctg cttggttacg ttatttactg gcagtcgatg atcaagggga aaattttgtg
    51421 ccaagtcctg atccgttatt ggtagagttg caggataaat tatcagtatt gaaattaggt
    51481 gaacaagatc cttcaaagat tcattgtgcg gtacaggaaa ttttagaaaa taaagaaatc
    51541 tttggtttag atttatatca agttggggta ggaaagaaaa ctgaaaaaat cttttgcgaa
    51601 atgctagtag gtcctcaagc agttcgtaaa acaatcaata aatatataac agaagttgga
    51661 ggaaagtaac tatggaaatg acttttagat ggtatggggc aaatgaagaa aaaatctcac
    51721 tacaagaaat tcgtcagatc ccagcaatga aaggaattgt tggaacatta atggatatac
    51781 cagccggaga ggtttggccg agagaacgaa ttaaatcttt aaaagatgaa gtcgaagcca
    51841 atggacttac cttgaaagtt attgagagtg ttaatgttca tgatgatatc aaaattggtt
    51901 taccaacacg agatcaatac attgagaatt ataaacagac gattcgcaat ttggcagaat
    51961 ttgggattga ggttatctgt tataatttta tgccagtatt tgattgggta aaatcggatt
    52021 tggattatcg tttagatgat gggtcttcta cgttagccta tatttcaaaa aatatcccta
    52081 ctgatccgga ggaaattatt agagaagtag aaaattctac taataatttt gaattgccgg
    52141 gttgggaacc tcatcggtta tcacaaatta agcaattatt agaagcctat aaagaagttg
    52201 atgaagagaa attacgagag aactttgcat attttctcaa agcaattatc ccgacatgtg
    52261 aagaagttgg gattaaaatg gcagttcacc cagatgatcc accatattcc attttcggat
    52321 tacctcgtat tgttaaaaat cgtgacgatt tagattggtt gtgtaatgta gtggattctc
    52381 cttctaatgg tattacatta tgtactggaa gtattgcaga acgaccagat aatgatgtct
    52441 atgaaattct atctgaattt tgtcaacggg atcggattcc ttttgcacat gttcgtaata
    52501 tcaaatattt aagtgacaat ggactcgatt tttacgaagc gccgcataaa tcagaatatg
    52561 gttctctaga tatgtataag atattaaaaa caatgtacga taatggattt aatggctata
    52621 ttcgtccaga tcatggtcgg atgatttggg gagaaaccgg tagacctggt tatggattat
    52681 atgaccgagc tctaggtgca gtttatttaa atggtatttg ggaagcgctt gaaaaggggg
    52741 cgagtaaata attatataag ggagtttatt tagaggaaaa attttctctt ctaaaaacta
    52801 acggaaggaa aggagtgttt aaatatgttt ttagataaga actttttatt agagaatgaa
    52861 atagctaaaa gactttttca taaatttgca aaacaccaac caattatcga ctatcattgt
    52921 catttagacc cagcatattt gctagaaaat aagcaataca agaatttgac tcaggtatgg
    52981 ttaaatgata aaggagctgg agatcattat aaatggcgat tgatgcgagc taatggtata
    53041 gaagaagagt tgatcagtgg tccaggtgat gattatgaaa aatttttagc ttttgtaaaa
    53101 acagtggaga aggcgccagg taatcctatt tttgaatggt ctcatttgga attacaacgt
    53161 tactttgata ttgatttgac aatttctgag aagaatgcac cagaaatttg gcgacgtgct
    53221 aacgaaaaaa ttcaaacgga ttcctatcgc ccgaaagaat tgttggagat gatggatgtg
    53281 aaagccatct gtacaacgga tgatcctaca gatgacttgt ggtatcatac tgagctttct
    53341 aaacaggata attctttcaa ggtattacct actttccgtc cagataaggt ctggagtatc
    53401 aaatcatcag gcttttcgaa ctatatttta aaacttagtc aaacagcggc tataccaatc
    53461 aaagacttta atgggttgaa gcaagcattg agccaacgag tggatcattt tcatcaaatt
    53521 ggctgtcgat tagcagatca cggtttgaat acttattttt atgaaatagc agatgaaaaa
    53581 atactcgaca atctgttaat aaaagcttta gcaggaaaaa cggatttcca tttatcagaa
    53641 attgcacaat ttgttacagg tattcaatta catttgatgt ctgagtatac aagatatggt
    53701 tggacgatgc aaatgcattt aaatgcttta agaaataatt caagtatatt aaaggaacaa
    53761 attggagtag atgtcggtgg tgattcgatg ggggatcaag ctagtttgac acaaaatgtt
    53821 gtagcattgt tcgataccgc tgaaaagagt gggtgcttac caaaaacaat tttatattca
    53881 ttaaatccaa ctgattggct tccactagca acagcaatgc aaagcttcca agggggtatg
    53941 attcaaaaaa tccaattggg atgtgcttgg tggttcaatg atacagctga aggaatgcgt
    54001 caacagttga ctacgatggc acaacaaagt ttgttgtcga attttacagg tatgttgact
    54061 gattctcgaa gttttctaag ctatcctcgc catgagtatt ttcgtcgagt attatgtcaa
    54121 ttactaagcg agtgggttaa agcaggccga attccagatg atgaaatgta tcttagtcag
    54181 atagtggaga atatttgtta tcgtaatgcg tataattatt ttaatttttt tgaaatcgga
    54241 agtagaaaat aatacttgaa ataaaagctt aaatttattc tttgtaagtt agtacagcta
    54301 gacaaagtat gtatccataa aggaaggaga tagatatgaa aaaagtagaa attttaagta
    54361 agttagaagc ggctggagtc attgcggttg tacgtgggaa aaataaggat gaggccatta
    54421 aagcaataaa ttcaattatt gcgggtggaa ttaaagggat tgaactaact tttacagttc
    54481 ctaatgctga agaaataatc aaagaaattg ctaaaaaata tgctgatcaa aaagatgtaa
    54541 taatcggagc cggtacggtt ttagatgcgt ttactgctcg tatcgcaatt atggctgggg
    54601 cagaatatat cgttagtcca tcttttgatc gacaaacagc tgagatttgt aatttatatc
    54661 aaattcctta tttaccaggt tgtatgacaa ttactgagat aaaggaggca cttactgctg
    54721 gcgtagatat cattaaacta ttcccaggtt ctgcttttgg tccatccatt gtaaaagcct
    54781 atcaagctcc attaccacac gtaaatataa tgcctactgg cggagtaagt atagagaact
    54841 tagaagattg gtttgaagta ggagttgttg cagttggtgt aggtgggaat ttattggctt
    54901 ctgttgaaaa aggtgatttt gaagaagtaa gtcgagtagc tagtaaatat atagataagt
    54961 tttatgagat aaagaattat taggagaaaa aacaaataat actctgaaat gtaaaaaata
    55021 aatactgact ctttacgctt cagagtatag tacgtacttt catcagaatg aaaacgctta
    55081 acaacagaag ggagagaatt gtatggaaag aaaatttggt ataagagaca aaataggata
    55141 tatgtttgga gattttggaa acgattttac tttcattttt gctagttctt ttttaatgat
    55201 cttttataca aaagtattgg ggataagcgg agctgccgtt ggaacattat ttttagtagc
    55261 aagatttatc gatgcagtta ctgatgtaac tatggggcgt attgttgata gatcgaaagc
    55321 aactaagcag ggtaagttta aaccgtggat tttaagaatg agtggtccag tcgctttagc
    55381 aagtttttta atgtatcaaa cttctatgat ttcagcttct atgagcgtta aggtagtata
    55441 catgtatgtt acgtatatat tatgggggag tattttttac acatctataa atattcctta
    55501 tggatcaatg gcatcagcta ttacttctat tacagaagaa agaacaatgc tatcgacatt
    55561 ccgaaatgtt ggagctactt tagcaagttt ggtcattggt gtattaacac cattatttat
    55621 ttatacaaga gatgattcag gcgctcaaat agttcgtggt ggatcaactt ttacaattgt
    55681 tgcgggaata ttttcgatat gtgcacttat ttgttattta atatgttata aactaactac
    55741 tgaacgagta agaattgacc aatctcaata caaagtttca ttgttaaaat ctttaaaagg
    55801 gttagttaaa aatagggcat tactttctat aattggtgcg gctattttct tactccttgc
    55861 tcaattatta attatgagta tgaataatta tgtatttcct gattactata atgatgcaca
    55921 gggaatagtt ttaatgaatt ttatcaatcc tattttggtt ttaatagttg tttcgccttt
    55981 aactttgttt ttaagtaaaa aatatggaaa aaaagaactg gcatcagtag gaatgttctt
    56041 ttcagcaata gtatatttga tcttattttt gataaaagct agtaatatgt atgtattctt
    56101 aattcttagt tcggttggct atatgggttt gggtgtgttt aatactgtta tttgggcaaa
    56161 tattactgat gttattgatg atcaagaagt aaagtctgat caaagagaag atgggaccgt
    56221 atatgcggta tattcttttg ctagaaaaat aggtcaagct ctagctggag gcgtcggagg
    56281 ttggactctt tctattatag gctatgactc tttagcaaat gtacaaacgg gagatgtttt
    56341 agaaaagtta tataacgctt caacattaat tccatcaatt tgttttctga ttgtaggatt
    56401 aattttattt ttcctttatc cattatctaa agataaagtc ttagaaaatt gcgagctttt
    56461 aaaacaaaaa cataaatagg agagaaatat gaataattgg atattttgta ttgattcaga
    56521 tggctgtgtt atggatacga tgacttataa acacaatctc tttttcggac caattgctgc
    56581 agagattttt gatgttcatc atagagaaaa attcctacag gattggaata atattaattt
    56641 atattcagag actcgtggaa taaacagatt tttaggttta gttaaaacat tagaaaaaaa
    56701 tgatgtaaca tcgattaatc gattaaagca ctgggtatct ctaacaaaag aattgtctaa
    56761 tccatctttg aagagggaaa tagaatattc tcagtcagag gatttattaa aagctttaaa
    56821 atggtcggaa gaagtcaatc aacagattag tcaagcaaaa ggattagata aacccttttt
    56881 aagtgctaga gatactataa aagctctgaa aaaatatggg aagataatta ttgtaagctc
    56941 agctaataag gaagctgttc aagaagaatg ggaaagacat gaattattat cattagttga
    57001 tgaactgtgt tgtcaagata aaggaaaaaa agaagatatt attcgaagtg taattgaaaa
    57061 tggttgtgat ttggataaga tattaatgat tggtgattct cctggtgatc tagaagctgc
    57121 aaataaaaat aaagttttct tttatcctat tttggtcaat aaagaaaaag aatcatggga
    57181 aaacttaaga actgatgttt tatttaaatg tttatcggga gattatgtag atattgaaca
    57241 aaaatatata gatacttttt ggaaaaatct tactaataag tagaggagaa agaaaatgac
    57301 tataaaagta gatttacgaa aaaaaccata taatttagat gaagatgcta tttcttggat
    57361 tgaagagacg attgattcta tgactttgga agaaaaaata ggtcaattgt ttgttaatat
    57421 gggatctagt cgtgataaaa attacttgag taatatggtc aatcagtacc atattggagc
    57481 tgtacgatat aatccaggaa ctgctgatga ggtatatgat caaaactata tcttacaaac
    57541 agaaagtaaa attcctctat taattgctgc taatacggaa gcaggtggta acggagcttg
    57601 tacagatggt actgaagttg gtgtagaagt aaaaattggt gcaacaaatg atgcgaaata
    57661 tgcttatgaa ttagggagag tttctgggat tgaagcatcg gctattggat gtaactggag
    57721 ttttgctcct attgtagata ttaatagaaa ttggagaaat cctatcattt ctagtcgatc
    57781 ttttggacaa aaccctgata aagttttaga acttagttta gcttatatga aaggtattca
    57841 ggagagtgga attttgcctg cagctaaaca tttccctggt gacggtattg acgaacgaga
    57901 tcagcatcta tcttttagta ttaatagttt aggtacggaa gaatgggata attcttttgg
    57961 aaaagtatac tcaggcttaa tagaagctgg attaccttca attatggcag gtcatatcca
    58021 tatgccagct tattcaaaac attttaatcc tgatctgaaa gatgaagatt tacttcctgc
    58081 aactttatca aaagaattaa tcactgactt acttagagga aaactcggat ttaatggatt
    58141 agtagttact gatgctagtc atatgttagg aatgactggg gcaatgaaac gatcagaatt
    58201 attgcccact tctattgcag ctggatgtga tctatttcta ttttttaatg atccagatga
    58261 agactttaat tatatgatgg aaggctatcg taaaggaatt atcactgatg atcggttatt
    58321 agatgcctta aaaagaattt tagggttaaa agctaaattg ggattacaca aaaaagcaaa
    58381 agaacaaatt ttattaccta agcaagaagc tctggaaaaa attggtttaa ttgaaaataa
    58441 atcaattttt aaagaggtgg ctgaccaagc aattacatta gttaaagata aacaagatat
    58501 ttggcctatt gtaccagaaa aatataaaag gattttacta gtagatgtac atggggtaaa
    58561 aggtgggttt ggtgaagtag ttgctggaaa tagacctcaa gccattgatt tattgaaaca
    58621 gattttgtca gcaaaaggat ttgaagttga aaaatacatt tcacctgaag aagaattgaa
    58681 aggattatcg caagaagaaa aaattaaaaa tatagctgga atatatgctg ctaagagacc
    58741 aattaaaaat ttaactaaaa aatatgactt gatcattaat atagctaatg ttaatccagg
    58801 cacagttcaa cgaattgtct ggccagcaag taaaggaact ccggatatac cgttttacat
    58861 tcatgaaatt ccaacaattt ttgtatctgt acagtgtccg tttcatttgg ctgatgtacc
    58921 tcaagtaaag acttatatta atacttatga tggaaaacaa gaaactttag aaatattggt
    58981 ggacaaacta atgggtcttt ctgaatttaa gggagtaagt ccaatagatg cttattgtgg
    59041 tttaaaggat acatttattt aatatttaga taggagtggt ttatatgttg tatcccatta
    59101 tgacagagac gaggcaagta attgacttaa gcggtctctg gagatttaaa ttagatacaa
    59161 atgatgactt acaggaagaa cttgctttag aaccaataaa tgaaaaagag agttatccga
    59221 tttcagttcc ctcttcttat aatgatttat ttgaaacaga gaacattaga aatcatgttg
    59281 gatgggtgtg gtatgaaaaa gaagtgactc ttcctaaaag attattagaa gaaagattag
    59341 ttcttcgttt tggatctgca acacatgaag caaaagtttt tttgaatgga aagtttatta
    59401 ctcaacacaa aggtgggttt acaccatttg agtctgaaat taatcggttt gtaaaagacg
    59461 ggaataatcg aattacagtt gctgtaaaca atattattga cgatagtact ttacctgtgg
    59521 ggattatgaa ggaaacagta caggaaaatg gggatataaa aaaggaaaac ttagttaatt
    59581 ttgatttctt caattatgca ggtattcata gacctgttaa gttatataca actccgaaag
    59641 actatataag tgatattact attaatacaa gaataactga tttggatgca agtgtagaat
    59701 tcaatatcga tacagaatgg accggaaaaa tcttaatagc gatatatgac gaagaggctc
    59761 aacttgtagc tgaagctgaa ggaaagaaag gaatgattca gcttaagaat ccaatttttt
    59821 gggaacctct agacgcttat ttatatagta tgaaaattag tttaattgat gataataaag
    59881 tgatcgatac ctatacagaa gaatttggta ttcgcagtgt tcaggttacc aatggcaaat
    59941 ttttgattaa taataaacct ttttatttta aaggatttgg taagcatgag gattcgtatg
    60001 ttaatggacg tggtttgaat gaggctgtaa atattaaaga ttttaattta atgaaatgga
    60061 taggtgctaa ttcttttaga acatcacatt atccttactc agaagaaatt atgcgattag
    60121 cagatcgaga aggtattgta gttattgatg aagtacctgc agtagggtta catttaaatt
    60181 tcatggccac tttattagat gataatgtgg aaaaacataa tacgtggaaa gagatacgaa
    60241 cagaaaattc tcataaaacg gtgatcaagg aattgataga acgcgacaag aaccacccaa
    60301 gtgttgtaat gtggtctatt gcaaatgaac cagattcgga ttccgaaggt gctaaagaat
    60361 attttgaacc tttagttgaa ttaactaaag aacttgatcc gcagaaaaga cctgtaacaa
    60421 tcgttactta tctaaaatca acgccagatg tttgtaaggt aggagatatt gtcgatgttt
    60481 tatgtttaaa tcgttattat ggttggtatg tttcgggagg ggatctggca catgcaaaat
    60541 atctgttgaa aaaagagcta gatggatgga tggaaagatg tcctgatacg ccaataatgt
    60601 ttacagaata tggagcagat acagtagctg gatttcatga tacagtacca acaatgttta
    60661 cagaagagta tcaagtggct tattacgaag caaatcacga agtaatagat acatgtgaaa
    60721 attttgttgg tgaacaagtg tggaattttg cagattttgc aacaagtcaa gggattttaa
    60781 gagtgcaagg aaataagaaa ggaatcttta ctagagatag aa
//
LOCUS       DACCSK010000004        54604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCSK010000004 DACCSK010000000
VERSION     DACCSK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.004"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IVP39_000212"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IVP39_000212"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7585317.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IVP39_000213"
     CDS             complement(860..1678)
                     /locus_tag="IVP39_000213"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7585318.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IVP39_000214"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IVP39_000214"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7585319.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IVP39_000215"
     CDS             complement(2967..3923)
                     /locus_tag="IVP39_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585320.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IVP39_000216"
     CDS             complement(3923..5056)
                     /locus_tag="IVP39_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585321.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IVP39_000217"
     CDS             complement(5049..6611)
                     /locus_tag="IVP39_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7585322.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IVP39_000218"
     CDS             complement(6823..7905)
                     /locus_tag="IVP39_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7585323.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IVP39_000219"
     CDS             complement(7971..8369)
                     /locus_tag="IVP39_000219"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7585324.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IVP39_000220"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IVP39_000220"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7585325.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IVP39_000221"
     CDS             complement(9098..10399)
                     /locus_tag="IVP39_000221"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7585326.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IVP39_000222"
     CDS             complement(10540..11556)
                     /locus_tag="IVP39_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7585327.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IVP39_000223"
     CDS             complement(11864..12469)
                     /locus_tag="IVP39_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7585328.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IVP39_000224"
     CDS             12665..13588
                     /locus_tag="IVP39_000224"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7585329.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IVP39_000225"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IVP39_000225"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7585330.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15280..16473)
                     /locus_tag="IVP39_000226"
     CDS             complement(15280..16473)
                     /locus_tag="IVP39_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7585331.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(16500..16700)
                     /locus_tag="IVP39_000227"
     CDS             complement(16500..16700)
                     /locus_tag="IVP39_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7585332.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            complement(17198..17428)
                     /locus_tag="IVP39_000228"
     CDS             complement(17198..17428)
                     /locus_tag="IVP39_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7585333.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            complement(17425..17847)
                     /locus_tag="IVP39_000229"
     CDS             complement(17425..17847)
                     /locus_tag="IVP39_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7585334.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            18378..18731
                     /locus_tag="IVP39_000230"
     CDS             18378..18731
                     /locus_tag="IVP39_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7585335.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(18789..18977)
                     /locus_tag="IVP39_000231"
     CDS             complement(18789..18977)
                     /locus_tag="IVP39_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7585336.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19075..20115)
                     /locus_tag="IVP39_000232"
     CDS             complement(19075..20115)
                     /locus_tag="IVP39_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7585337.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20477..20974
                     /gene="dfrG"
                     /locus_tag="IVP39_000233"
     CDS             20477..20974
                     /gene="dfrG"
                     /locus_tag="IVP39_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7585338.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(21046..22998)
                     /locus_tag="IVP39_000234"
     CDS             complement(21046..22998)
                     /locus_tag="IVP39_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7585339.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(23005..23301)
                     /locus_tag="IVP39_000235"
     CDS             complement(23005..23301)
                     /locus_tag="IVP39_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585340.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(23476..24279)
                     /locus_tag="IVP39_000236"
     CDS             complement(23476..24279)
                     /locus_tag="IVP39_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7585341.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(24295..24411)
                     /locus_tag="IVP39_000237"
     CDS             complement(24295..24411)
                     /locus_tag="IVP39_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7585342.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(24656..25585)
                     /locus_tag="IVP39_000238"
     CDS             complement(24656..25585)
                     /locus_tag="IVP39_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7585343.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(25602..26624)
                     /locus_tag="IVP39_000239"
     CDS             complement(25602..26624)
                     /locus_tag="IVP39_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7585344.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(26621..28648)
                     /locus_tag="IVP39_000240"
     CDS             complement(26621..28648)
                     /locus_tag="IVP39_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585345.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(28645..31098)
                     /locus_tag="IVP39_000241"
     CDS             complement(28645..31098)
                     /locus_tag="IVP39_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7585346.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(31082..31477)
                     /locus_tag="IVP39_000242"
     CDS             complement(31082..31477)
                     /locus_tag="IVP39_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7585347.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(31549..32187)
                     /locus_tag="IVP39_000243"
     CDS             complement(31549..32187)
                     /locus_tag="IVP39_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585348.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(32243..32737)
                     /locus_tag="IVP39_000244"
     CDS             complement(32243..32737)
                     /locus_tag="IVP39_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7585349.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(32804..33583)
                     /locus_tag="IVP39_000245"
     CDS             complement(32804..33583)
                     /locus_tag="IVP39_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP7585350.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            complement(33625..33846)
                     /locus_tag="IVP39_000246"
     CDS             complement(33625..33846)
                     /locus_tag="IVP39_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585351.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(33843..34133)
                     /locus_tag="IVP39_000247"
     CDS             complement(33843..34133)
                     /locus_tag="IVP39_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585352.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(34130..35314)
                     /locus_tag="IVP39_000248"
     CDS             complement(34130..35314)
                     /locus_tag="IVP39_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7585353.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(35496..36899)
                     /locus_tag="IVP39_000249"
     CDS             complement(35496..36899)
                     /locus_tag="IVP39_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7585354.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(36921..37694)
                     /locus_tag="IVP39_000250"
     CDS             complement(36921..37694)
                     /locus_tag="IVP39_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585355.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(37704..38081)
                     /locus_tag="IVP39_000251"
     CDS             complement(37704..38081)
                     /locus_tag="IVP39_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7585356.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(38102..38416)
                     /locus_tag="IVP39_000252"
     CDS             complement(38102..38416)
                     /locus_tag="IVP39_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7585357.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(38616..40409)
                     /locus_tag="IVP39_000253"
     CDS             complement(38616..40409)
                     /locus_tag="IVP39_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7585358.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            complement(40406..42595)
                     /locus_tag="IVP39_000254"
     CDS             complement(40406..42595)
                     /locus_tag="IVP39_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7585359.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(42729..43919)
                     /locus_tag="IVP39_000255"
     CDS             complement(42729..43919)
                     /locus_tag="IVP39_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP7585360.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(44791..46422)
                     /locus_tag="IVP39_000256"
     CDS             complement(44791..46422)
                     /locus_tag="IVP39_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7585361.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(46436..48109)
                     /locus_tag="IVP39_000257"
     CDS             complement(46436..48109)
                     /locus_tag="IVP39_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7585362.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(48122..48439)
                     /locus_tag="IVP39_000258"
     CDS             complement(48122..48439)
                     /locus_tag="IVP39_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7585363.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(48464..49864)
                     /gene="lacG"
                     /locus_tag="IVP39_000259"
     CDS             complement(48464..49864)
                     /gene="lacG"
                     /locus_tag="IVP39_000259"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7585364.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            50101..50886
                     /locus_tag="IVP39_000260"
     CDS             50101..50886
                     /locus_tag="IVP39_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7585365.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            50889..51581
                     /locus_tag="IVP39_000261"
     CDS             50889..51581
                     /locus_tag="IVP39_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7585366.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(51855..52226)
                     /locus_tag="IVP39_000262"
     CDS             complement(51855..52226)
                     /locus_tag="IVP39_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7585367.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(52626..52856)
                     /locus_tag="IVP39_000263"
     CDS             complement(52626..52856)
                     /locus_tag="IVP39_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585368.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(52950..53288)
                     /locus_tag="IVP39_000264"
     CDS             complement(52950..53288)
                     /locus_tag="IVP39_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585369.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(54216..54332)
                     /locus_tag="IVP39_000265"
     CDS             complement(54216..54332)
                     /locus_tag="IVP39_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7585370.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT        16208 a        11105 c         9230 g        18061 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aatgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcacta attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggctcctagc ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagt agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatccg ttcggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcga tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat agttgtagga gagctagaaa tcctcttata
    15241 ccaagggttt ctggctcttc ttatcttttt actgcacttt tattgcactt ttttcatttc
    15301 tttgtttcgc aagtagtcta cagtaaattg attctttggt gaagtatcat aattttgttt
    15361 tgcttcttta aaggaaatgg ctccatttaa tttgttagct acctcgaaat tactgttagg
    15421 ataaagatgt ccatacgttc ctaaagtcgt ctcaatatct tcgtgtccta gacgatcttt
    15481 aatgataagt ggattttctc ccatactgat taacaaggaa gcatgagaat gccgtaaccc
    15541 atggattctg attcgatgga taccagccag cttagcatag cgttcaatag catatgatag
    15601 tgtatgtttt tgggtaggaa taccgttata gctcattaca aagtctgtct gaattagatt
    15661 ttgttgtact tccttccatt cggataaata agttaatgtg catttatcta atacaatatg
    15721 acgaacgctc gcctttgttt ttggttcaac aaatctataa ttcgattgat tcttgtaata
    15781 gagtgttttg ttgatggtta gtactcctga gtcaaaatca atatcttccc attgaatggc
    15841 agtagcttca ccaattcgca ttccagtcat aaataaaaac cacagagaga taaataaaaa
    15901 gtgttgatag taatcttctt tgtaaattag agagattact ttttcaaatt cttcttttgt
    15961 ccaaaattca attttagact tttgcttttt cacattgcca ataatcttag atggatttgt
    16021 ggtggttatc ccaaggacaa ttgctcgatc catagcaact gaaaatagtc cttgaacagc
    16081 tcttacataa gaagacttac acttctttga taattttagt tgccaatttt gcacatcaat
    16141 aggctcaata tcggaaacag ccattttatc aaaataagag aaatgcttct taatagttgg
    16201 taagcgattt tcatatgttc tgagctttac ctgtgtctta taccatggaa ggaaaatttc
    16261 aaagatataa tgtttaaacg tcattttatc cttattctca cttaattctt ctggtttcat
    16321 caatagcagt ttcgaatatt cttctcttgc ttctttttta gtagtgaatc cactacgata
    16381 tttttgaatt tttttccctt tagaatcata acctagattt gcacgaaaat aataggttcc
    16441 attctttgct ttttttattg ggtctttaac catagttttt actccttact gtgcaaaaac
    16501 tactcagacc tgttttcatt tgaaaaccga attcctagaa tttcttcaac agcttcacga
    16561 gggactctat ctagctttcg agattgatag tagatatgcc cttttgtaat catcaattct
    16621 tttgcttttt ttatgatgtc tgctgaaaaa gaagttccat atcctagctc aactaaatcc
    16681 ttttttgtga ccgttatcat tattactcct ttcccaatta taggctagga aaactccaat
    16741 attcacctat aattatatca aatagtttta taataataca ctgacaagag aagaatatct
    16801 tagcaatcga acagctctag caaagctcac aggaatttca cttctgcatg gttctcatgt
    16861 agccgtaccc attgcctgtg acgcttctaa cgctcggact atggctatac gggagtatca
    16921 ttatcacgat aagaaagtca tggcagcaat cctgctaaag attactttcg aattactaac
    16981 atgaatcgct cactaatttt gtaggtcatg gcgtgttagt tcatcagctc ttttcttacc
    17041 gaaacgtatt cgattgcctt tatttggttg tcaaagaacg tttggcttgt tagcctatca
    17101 aaacctactg aacacacaat atgctttgat ggaataacaa acctcccaaa tcgggaagcg
    17161 tggatacgat tcgacacgct tccacgaaaa aatgggacta tttaatttcg aacgataaaa
    17221 tcttagagat gagtttcgtt tccattctgc cacgtagggt ttcatcaata accatatgag
    17281 aatttccata ttcgtctgtc atgcgacgta atgaacgttt tgaggtatac cctcgataat
    17341 gtttaacaat ctgattaatt gcttccacgt cgccatctgt cgcctttaca atgaggggga
    17401 agggaaccct tggataagct gttttcattc tttacactcc ttcatgaatt ctttaatcat
    17461 ggctagtcca cttattcgat gacgataaac agtcgaacga ttcaaattta gtaactccgc
    17521 aatctccaca tcactcatat ccataaagta atgaagtaaa ataatgttcc gttttttctc
    17581 tgaaagatga cgcagggctt cacttaataa atcactttca acattgattc ttaatccaaa
    17641 taatttaaat agttggaaat cagtgacgta cgtatcaaca gtagaaaaag agttgacaag
    17701 gtagttatct atatcagaaa acgagacttc ttttgctgat agtctgtcta gatgtttgaa
    17761 gtaatctttt cgttcatctt caataacttg tttgcagata tagtcaaact gattttctat
    17821 ggtttcttga aaagaagatg gtttcatgct tttcaccccc tttctgtcgt gaaagggagt
    17881 ggcatgtgct tctttatctc ccttcatact aaaccccgac acgaacgggg gatttgttgc
    17941 ataaatgaag agaaatccta aaagatattt attaagtgac caaaaaagca cataaacaga
    18001 ttgttttctc tgttcatatg ctttgatttt tctatctatg taatctgcaa aaccacattg
    18061 atggtcatat ctatccagta caggtggata agtttttttg ataagaactt aattaagaga
    18121 actagatgac agattatctt tctcatggcg acttttccct ccattaagtc gccaatttta
    18181 tctactttct aggagtatga ctaaatttta taaagttgta taggtaaata aaatcagcga
    18241 cctttctatt tgaaattgga ttcatcgcat taataaattc cacagtgttc tataggaact
    18301 cataaactat ataacatgtt tttctatacc tgtttatggt tatataggaa cagtaaaatg
    18361 tatagaggtg atttagtatg cgtaaaaaag aagataaata cgattttaga gccgttggtt
    18421 tagcgattaa agaggctcga atgaaacggg gtctcactcg tgaacaagtg ggaacaatga
    18481 ttgaaattga cccacgttat ttaacaaata tagaaaacaa aggacaacac ccaagtacac
    18541 aagtgctgta tgatcttgta tcattactcc atgtatctat tgatgaattt ttcttaccta
    18601 cagataattt gattaaaagc actcgaaggt tacaggtaga aaaatacatg gatagcttta
    18661 cagacaaaga actatcctta atggaagcac tagcaaaagg tatcaatgaa gcaagaaata
    18721 ttgaaggcta gtcagtaaaa ttcagataaa caaaaagagc cgataagatg agagtttcat
    18781 ttctcaaatt atcggctctg cgtcttggcg tctggctctt tgattgttat tatattcatt
    18841 ttttgaacat taattaaagt ttcgttctta catttggggc agtataaagg gaaattttta
    18901 agtatagtat ccactcgtat tcgtagtctt gttttatttc cacaaatagg acacaatatc
    18961 cacttgtagt ttataataac aatctcctcc tttccacttt aattcaaatc tatattaaag
    19021 aatatttcat cttatttaat aagaaaccat atttatataa caacataaaa cgcactaagt
    19081 tattttattg aacatatatc ttactttatc tatccgacta tttagacgac gggtctggca
    19141 aacaggttcg ccagtggtaa cctgatatcc ttttagctct gctaaacaaa cactaagccc
    19201 atttgtaaaa aaagttaaat cattgcgata atcttgaata catcgagcag gaatttctcc
    19261 aataataatg acctcattat ttttcagttg agtatttacg atatttgcac aatatttggg
    19321 agcatcgtta tatgcccgtg aaagatattc ctgtggtgca taaactttaa aactaagata
    19381 tggctctaac aattctgttc cagcttttct aaaggcttgc tccagtacaa taggagtaag
    19441 catccgaaaa tctgctggag tactaacagg gctatagtat aaaccgtact taaaacagat
    19501 tttacaatcc gtcacattcc aaccatataa tccttgttcg caaccatagc gtatcccttc
    19561 cataactgca ttttgaaatg attgatttaa gtatccaaga gaaaccgagc tctcatactg
    19621 cattccactt cccaacggaa gcggtgatac agataaacca atggaagccc agaaaggatt
    19681 tggcggcact tcgatgtgaa tggtatattc tgcatttttt aacggtctct ccatataaat
    19741 gactgtaggc tcttttagtt ctatctccac atgatacttt tcttgcaaca gtgcactaat
    19801 cacttccatt tgtactttcc ctaagaaaga aagtataatt tcatgtgtcg tagaatccac
    19861 gtaatatcgt agaagcggat cactatctga gatttccaaa agggcatcaa gcaacatttc
    19921 tctctgttca ggtttactcg gttcaacagt tgtttgtagt agagggtgcg gattttcaat
    19981 cttttttctc tgtggcaata gttttgtatc tccaagaaca ctatttaact tcaaaaactc
    20041 attttgcaaa ataacaattt ctccagaata agctctatca atcttacata attcaccatt
    20101 tattgaagta tacatttctg taacttttat tttttctttt tctgatactc taaccgaatc
    20161 tcgtaaatgt agtactccac tataaaggcg tatatatgca agtaagtagt tcagttttgg
    20221 agtacaaaat gacaaaatgt acttcaaaat ggactacttt tttcatttta acttacatac
    20281 caaattacat acaaaatgat ttataaaatg caattgaaaa tgataattga atgaaaattt
    20341 gtgattttgt ggtattatga taatataact tatagaaaaa attgcttatt caactaacgg
    20401 gcaggataat gagaaaaggt tttttgatag aaataagaaa ttagatggta aattagttaa
    20461 aaaggctgtg ttataaatga aagtttcttt gattgctgcg atggataaga atagagtgat
    20521 tggcaaagag aatgacattc cttggaggat tcccaaggac tgggaatatg ttaaaaatac
    20581 tacaaaggga catccgataa tattaggtag gaagaacctt gaatcaatcg gaagagcctt
    20641 acctgacaga agaaatatta ttctgacgag agataagggg tttaccttta atggttgtga
    20701 aattgttcat tcaatagaag atgtttttga gttatgtaaa aacgaagaag aaatttttat
    20761 tttcggagga gaacagattt ataatttgtt tttcccttat gttgagaaaa tgtacatcac
    20821 aaaaatacat catgaattcg aaggagatac tttttttcca gaagtgaatt atgaggaatg
    20881 gaatgaggta tttgcccaaa aagggataaa gaatgataaa aatccgtata actactattt
    20941 tcatgtatat gaaagaaaaa acttattgag ttaacgggtg cgttaattga agaagaacag
    21001 taaagataaa gcacaagagc ttgggattac tccaagctct ttttgtcatt tgaaataaat
    21061 tatatctttc caatcaaatt gtttatctgt aatccctaat ctttgagcag gtgtcaaaac
    21121 ctttttatcc gctgatttaa aagggcgaca aaagttgtag taagttctta gaattgtaat
    21181 cgcaaattga gcatacttcg gattgaagtt agcgtaaata tagctcttct tatcaccacg
    21241 agctgttgtt aatggtcttt caagagatga tatataacgt cttatctgtt gaataaagct
    21301 attggtcgaa tggtcattaa catttaatag catctttgcg atttcgtttg gttccagagc
    21361 agataaatcc gttctacaat caactgaata aaatccttta tccttagtgg ctaatggatg
    21421 tttgataggg ttatcggcat atttgcgata tctttctcca tctttactaa tagcctcttc
    21481 gtggaattga tgacttgtta gtaattcagt tagataatga gatgccaaag tccgtagaaa
    21541 ctttgttttg aatcctctga tatctcccca atcgagcagt tctgcttttg cttgtccgaa
    21601 ttcttttaag cattgttttc tgcttttcgt cttgttaact tggcaaataa aatgatgtgc
    21661 atcgctcaac cttaattctt tcgtgaatac tctataaaag gcgtttctaa tagagaagtc
    21721 atcatcacta atcattcgcc attcgtcggc attaaccatt tgtttaatta accaatagtg
    21781 agcagttgta gtataaggag catcaacatt taaaccatcg acaaatctac tcctattagt
    21841 aattttccct aattcgtgaa gataagcatt cctattttcg ctatcgttag cggatggttc
    21901 ttgtggataa taagaccaat ctaacctatc atttttacga ctaaaagtat tgagatggtc
    21961 ttctttgaat tttcttgtat cttcatttaa ttcatccatt gaaatattcc aatcataggc
    22021 aacatcagaa cggaacacat atcttgataa gacctcagca gatacaacta tatacgtttg
    22081 catattaagg tcttcaaacc ccgtatattt ctttgaacct tgtcctttct tacggacgtt
    22141 atttaggtag tagtgcatct tgtccgtatt aagccacatc tcgttaaatt tctttgtttg
    22201 aaggggttgt gtctcatatc gttctaaaaa ctccaaacaa cgcctataca gccactccag
    22261 cttatgatag taggttccaa ctccaattcc taaaatatca caagtacgac tgactgaaac
    22321 ccttccaaca accatcttag cgaacattgg aagaatggtg ttcttttgtt ggtgataagt
    22381 agtggtttct tctctttttg gtagaacatt agtgaacttc ttacacgcct tacattgata
    22441 gcgttgagat ttgcccctac ttttccccct tttgtaaaat tgcttcggct cattgaatgg
    22501 ggttgattct tcttccgagc agccttcttt atgaaattga taatctggct caacatcttg
    22561 gatgctattg atttctataa ggcgtttaat ttcttcaaca acagaccaat tagataaagg
    22621 ggttactgta cagttttgcg atacacctct gccaatagga ttgtcattgc agtacatacc
    22681 tttatgacct ttatctttgg aactaccagt cattttatat cgacttggtt tcccttttac
    22741 atctttgaat ttgtgctgtt ctttcccaaa attacaacaa aacggattga tgcagtaatt
    22801 atattgaatg ggatattcag aaccgttcca tttaaaagac gttggcaaga acaacatttt
    22861 gttatacctt gttgcaaatc gttttggagt taataaatca tattgtttag ccctatcttt
    22921 taattcttcg tttgaaatcg gaattttaac atctacaatt tcatcttcct tcgtggctaa
    22981 tcttttaagc ttggtcattg tctatcaacc cactctatct ttgataccca ttttttcaac
    23041 aatccgctta ttacttcgct tgttggcaat ccattcaata aacttcttat cgtctaaaat
    23101 gttcaataag aattcatcca ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg
    23161 cttaactatc acccttactt gctctgatat ttcccaatcc acagacttag cgttcttatt
    23221 ttttggctca agaaattgca taggaaaatg cacccctttt tgcttagtaa attgtaacca
    23281 ttttaacaca ccaatttgca ttcctacaat aattatttca cgttcgcatt tttatataca
    23341 ttttaactta cattttgtgg tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg
    23401 agtatcaaat tcagttgcaa tttgatactc atttaggttt acattttcac tttttgtact
    23461 ccaaaactga actacttaat atgcaagacg ttgtcttttt tttgtatatt caattttgaa
    23521 aacatttccg caaagttcag acggacctcg atgtgttgat gaataaaatt tattagtaat
    23581 aacttctata aggttatcaa tccctatatt actttttgca cttccatgat aaagagggaa
    23641 cagagaacaa ttctgaaatc ttatgctttc ctcttgttcg agttccaatg cttctaatga
    23701 tttaccggac atatatttct ctaaaaggtc atcgtttccc tctattaccg tatcccattg
    23761 ttcagattcg gtaaagttcg tcacacacat attaggatac agttctacct tctgtttgat
    23821 tacaatttcg gcagaaagtt tctctttaat atcctgataa accgttgata aatcaattcc
    23881 attttggtca atcttattga taaaaaagat tgtgggaatc cccattttcc taagtgcatg
    23941 aaataatata cgagtttgtg cttgtacgcc atcttttgca gaaatcagta gaattgcccc
    24001 atctaaaact gataatgaac gatatacttc tgctaagaaa tccatatgtc ctggcgtgtc
    24061 tatgatgttc accttcgtat tttcccactg aaaagaggtt attcctgtct gaattgtaat
    24121 tcctctctga cgttctaaaa gcgtattatc cgtcctcgtt gtacctttgt ccacgcttcc
    24181 taattctgta atcgctccac tgttatataa taagctttct gttaaggtag tttttcctgc
    24241 atcaacatga gctaaaactc caatattaat aattttcatg tgattttcct ccattcaaaa
    24301 acccaaaagg gcataaaaat cccagtgata aatactttta tcactgggat ttttatgcat
    24361 aaccataggt atacaaagca tacagatatt ctctggatac tttagaatca catgataaag
    24421 gtattcttaa actgggtaca aaaaactaag ccctcctaaa aaaggacatc taattatttg
    24481 ttcccgctat caaattgaca gtttatttaa gaataccttg ccgcatattt attaactcct
    24541 tttaaatagt cacttaaata atagcacgta agagcatatt tgtaaaggaa tctccaattt
    24601 tttatcaaaa agagtacatg attacaaagt atctgtaatc atgtaccaat atttgttatt
    24661 ttacaatctt ccaattatct tgtttttcta atatcaactc aaattgcgag atttgggttg
    24721 cctttgtttc ttgatctaaa tatttcacag cgacattcac gattacttgg ttatcttttc
    24781 tggtaaagat aggattcacc aattcctcga aaacatattc cttgttaatc ataggtaaca
    24841 catgattact tacatagtaa gtcaattctt tttctgtggc ggtaggatag agctggaaga
    24901 acgtttctaa aaagctagtg atttcgtcca tcgtttctgt atctactgta tggtcacttt
    24961 ctagctgttt gggttggtag tccgattttt gaggtttctt actcattgtc ggatttttga
    25021 taataaccat attatctgat tcatctatat ggacaaccac atgaaagcta gacgatatag
    25081 tttccttgtc tttgtcttcg gtaataactt gttcaacaga aaacagtacc tcaaaggtgt
    25141 tctcattcac ttgagaaacc tgccacacct gtatatcgtt cacactagaa ctagtaggaa
    25201 tatccttacg aatcatttct tcatttaata cctgcaattc ttccgtcaaa taatgggtca
    25261 atttttcatt tcgtttatca atggcttctt gggattgttg ccacgaaaag tagtctttcg
    25321 caaaggattt cacaaaactt tctacttggt tggtatcgac aatctgctgt ttaatgattt
    25381 ctgtttcacg gacagtatga gtgtcaatgg cagtaaaatt tttatagatt ccaaatccga
    25441 cactaccaat caataaaacc cataaaacaa aggttaattt cttatgcgtg ccaactttaa
    25501 tcatcggaac ttttctttgc ttgctcggtt ttttagattt ctttggtttc tccttctttt
    25561 cttttcgctc gatttttata atcatttgat tcattccttt cttagttatg aataacacgt
    25621 ctagccccaa tcagatggtc ttgccaataa cgactgctta agtcgccata accgattggg
    25681 tcgcctgcat gataaaagcg atttccctct aaatatatcg ccacatgagt cacatatgtg
    25741 cctgcattat aggttgaatg aaagaagata aggtcgcctg cttgggcttc ttccatagag
    25801 atttcttgcg tagcgtcata ctgatcttgg gcgacacgag ggagagaaat gccagctttg
    25861 tcatacaccc attgtattaa gccactacaa tcaaaagagg tggtaggcga cgcccctcca
    25921 aagacatagg gaaacccttc gtatttcaag gcttcgtcca taatgacttg cacggtttca
    25981 tcatcaaatt ctgttgggga agtgtcggtt aggtattgtg aaaccaactg aacataaaac
    26041 tgatttccat agttatatcg ccaaccacca ttcacaggta tggctatagg attgggatag
    26101 tctgcttttt gaccacccga cttctcttta gaaaactgtt cggctaattc ataggtatat
    26161 tttttcccat gactcctcac ataatttaaa aagccaccgc cataattata agattggata
    26221 acagaatcta tatccactcc ttgttgttct gcactggtta ataattcact gaaatattta
    26281 accccttgtt tgatggactc ttcggtactc aaactgttgg gcggtaaacc caaagattcg
    26341 gaactttgca taacatcttc tgctgtaccg cccgattcca cttgcataat ggctaagata
    26401 taagaaacat agtcttcaat cccatattcc ttaccatatt tttcaatcaa cggacggtgt
    26461 gccaacactt ctggtgaaac ggtcacaccg ccttgtggaa tagaagaatc cttgttgttt
    26521 tcgccactgt ccgtatcatc tgcaaagaaa atgactagga ataataagag aaaaaagaag
    26581 agagagaaga ccacactaca aatcactgtt atttttagct tcatgtttgt tgtcctcgct
    26641 tatccgttct tttatttagc ttcctctgat tgaattgctt cggtggtgtc ttttgttttt
    26701 gctctttttt caatggtcgc tgatgatttt cacgtttagt gttttgtgac aaaacaggcg
    26761 tgacggtttg ctgacgcttg cgaacatgct gctggttatt ctgaacagga gatgatgggt
    26821 tttcttttcg tcttttttct tttgctttat ccagctccaa acgcttttga gcaatgttct
    26881 ttcgtttttg ctcctgctgg ttcatacgct gttgtttgcg ttgagctttg gtatctgcca
    26941 tagattgttt aaagtctgtt acattatgag tgacattctc ttttccttga tgaagggcat
    27001 atttgacatg ggttggtgtc gcctgtatct gttgttttgc ctgctttctt ttattgacta
    27061 gacgattttt cgtgtccaat acatgggctg tcttttcacc taatcgttgc ccgataccgc
    27121 cttgttgctt tttcacattg gaaagtgctg tttctttctt tggtgtagtc cgttcatttt
    27181 gacgagtatg gttagcttta ttggtttgat tctgctttgt tgtcattgtc ttttgcttcg
    27241 ttgaccctgc ttttccacga aaaatcttac tcatactgcg ttgaaatctt ctggtttgtc
    27301 gattcatcat ttgacgaggt ctgcgaaaca cttgtctgcc catgttttga gagtcgttgc
    27361 tttgtaaagt gaataggctc attaaatcgc ccagtttaaa ataaatccct gcaaaggtca
    27421 caatttgtaa aaacataatc atgaaaaagg gagaacttgc tgacagcgaa taaagcatag
    27481 tggaaatact aaaggcaacg gtaataatca aggtaatacc agcccttgtc ataatcacat
    27541 taaataactt ggtaatggca tgcctgccca tgccatcaaa actcggcaac atacttaata
    27601 ggaaactgat tggcaaaaac atagcgaaaa taataaatag cacttgcgaa aaaatcatga
    27661 ttcctgttag aaggaaaaca aaaatcgaga taccaatatt gaatacaaac aaaaagaaga
    27721 ctgttcctaa tctggaaatc gtcttcggta gggttaaata ggtattgtca tgatcttcaa
    27781 tttcttcaat cactacattt tctctatctt caccattgtt tgtatctgga ctggtagaca
    27841 ataaactttc cacacgttct tcccctaacg cttctacatc agtggagcca tattgaagca
    27901 atagccatgg ttgctttact tgaacagaga ataagctgtc acgtatcatg tccacactgt
    27961 ctttcccttg actatccgtg ttaggaagaa caatttttgt cccaatattc agactggcgg
    28021 tactgacatc tttggaaaaa tcattgattt tcgcaatata atcgggtgca tacgcaataa
    28081 aggaagccga tagaataaac accacgacaa agtttaaaac agctcgtatc actttcgtcg
    28141 tttctcgctt aaccaatcct gtataggcaa catagattcc aacaatgaga ataaaaagaa
    28201 ggaggaaacc gacataaaac cctgaactgg aaaagccatt cggtgtaatg cctgcaatcg
    28261 tctgcatgtt ttcgccaatt gcttgggatg tttgactaat gaaatctaag gcataggctt
    28321 ctttcaccaa ataacctgtg gcgttcgata gataaagaga aattgtccaa ataaaattag
    28381 taatggcata caatccatac atcaccgatt gtcctatgcc atcactccaa ttccatggaa
    28441 gccagcccca gctattgtcc acataataat caagctgata ttgattcaaa ccatacttgg
    28501 agtattcatt gcttgtatca atggtttcat caactaagcc tgtcgcttgt actgcattgc
    28561 ccagcgtgcc taataaaagt aaaataacgc ccacacaaag gagcgttatc caactatagt
    28621 gaagaaattt cttttgtttt tgtgtcattc ttccacctca tttcgcacag gcggtctggt
    28681 atcaaaggca tgaagcaact cttcaaaaat cgggtgaaac tgcataaccc ctacacgacc
    28741 atagatgtct gaaactaaac attgtccatt ttctaattca cgtaagcgtt tctgattgtt
    28801 ttcatcttcc ttgtccacac caaaaaattc taaggtcttt ttgatttcca ctaggtcagt
    28861 ggaacgaaag gcaaatttca agccaatatt attcttcatc ttttcatcta gtaaatcgtc
    28921 cgcgttttga gtaacgaaat agacccctgc attcatggca cgtcctgctc gaaccaatcg
    28981 catagataga gcttttcctt gtgccacttg taaaaaactc caagcctcat caaggtcaac
    29041 aattttaaaa atggaacggt cagagtggat aaagtctaaa gcaaaggtac taatgacaat
    29101 cagcattgcc acacttaata attccatggt agtgtattct tcaaaagagc tattggaatc
    29161 gggtaagacc aaatctgcca cttgaataat attgagttgt ttttccaagc taatggattg
    29221 ggtgacactg ccatcactga ataacagatg ggcaaaatca taatcgacaa agctttcgat
    29281 atggtcagca atactattgc tgatgggcgt atcttctttt cgcaattcat caatcaccaa
    29341 taacaaccct cgttgtttgc tttgagttac actccgaata gctttacgca ggacaggaaa
    29401 cttctcgcta tctcggctgg aaatacctgt taagaacgtg agaatatcaa tggcaaggct
    29461 ttctgaatct ttcgggagtt ttaaaatcac ataagggtca agtaaccctt tattgtcgtt
    29521 ctcactcgtg agattaacaa tattgatttc atgggcaata tcaggtaagg tttctttcca
    29581 ttcaccacgt tcagcttttg ggtcaacaat ggttgcctgt ccgccaaaga gtactgaata
    29641 atagaccagc atattgttag agaatgattt tccgccacct aatgaaccaa taaaagcgga
    29701 cgctaatgca tttgttacag agcctttaat tccttgactt gcaaggctag gttggagata
    29761 aatatttctg cctgtatcga gattgtaacc gacgtaaata ccagaatttt caccaatcat
    29821 ttgtgtagca ccgaaaccaa gaccagccaa aaaatccgac gtgacatatt gaatatagtc
    29881 attgatatat cgcttgcttg agggaataaa ttcattatgc aagcctagca tatcgccaaa
    29941 aggtcgtact aatttgatag acagatcatc ataaaaatct tttacttcat cacaacgttg
    30001 tttcagctcg tccaaattct ttgctgatac ccgtaccaca tagcttagtt tgtacataga
    30061 ttctttggtt tggtctaaat tactttctaa ttcatcgaca gattctaaag cgtccaccac
    30121 attagtactg gtttcgttgt cgctttccca tgcgtggcta tctaaatctt tcagttcttt
    30181 ctttttattg cgaacggtag ataaggcttt tttattggcg acaatttcaa cattcatact
    30241 ggtatcaacg gggaaagtaa actgctgttg ctggtaataa aaaatttcac tattagggaa
    30301 atccaattca ccaacaatac tattaattgt aaaatacgcc acataagtag tggtatcttc
    30361 ctgttcaatc cgtaaatagc gttgattttc ttcgattaaa cagcgagtgg gtttgagaat
    30421 atcgtatttc tttaccaaca cttttttatc ttcgtatcgt ttcggtaaat ggtagtcgta
    30481 gtcatcaaaa gggacacctg tttgcccgta aagatgttca ataatataac caaaatcatc
    30541 tttctccaat ttacgaatct gaaagcgtct ggataattta ctttctaaca actgttccat
    30601 cttagaaaag cgttcgattt ccgccatagg catagaaaca aaatcgccca ttaatttgtg
    30661 attcacacca tgaaagaatt ccgcaaatgc tttttgaatt tctttcccaa cattttttac
    30721 ggatacttcc tgttcattga ggtagagttt aaaaccaata aaaaagcgat agtctacttg
    30781 attgtcacca atcatagaaa ttaacgcttc ggtctgttca tctatttttt cgtaagctat
    30841 ctcttttaat cgtccagtga tttctttttt cgattgttcc tgtgtggctc gaatacttga
    30901 ttcggttgct agttgcagag catgaatctt gccatcacga ttttgggcaa tcaattgaca
    30961 gaaattatca tgaacttgaa ttttttcatc ggaacttaaa aaactgtaat tatagggaat
    31021 taattcataa taagcgaata cttcgccatc atgattaaat acgagattat cttccatata
    31081 tttaatcgga tatgccatct agttgttcac tccttacaat cgtaatcgat tcattcattg
    31141 tttctttttt taatcgtatt tgtttgccag cgtaggttac tttagggcgt agagcatagg
    31201 taatgataga ctttagaaat ccatagggtt tctttccgtc aaaggtcttt tggcacatga
    31261 acccagtgag aacaatggga atccctaagt atttgagtag tgctccgtca atcagagaaa
    31321 gtggtggtaa actgccaaag agtatgacga caaacaaaga aacgataaac catgtcattt
    31381 gcgtaaaggt aattgggaaa gggagttgta aatcattaat cgcataaatc actttttcta
    31441 ctgaccaaat actggtgtaa cttcttattt ttttcatgtc ttcacgtcct ttccgtaaaa
    31501 atagaaaaag aacaatcaaa ataggtgatt gctctttcaa tgctttaact attcaatttt
    31561 attttttgtc cattttgcaa taataccttc atcagtttct tcatcatctg gattcattga
    31621 atatctgaaa cttaaattgg tatctgaccc taatcgttca tataagctgg gtctatacaa
    31681 tctcgtttta ttgtcattaa aaggacttcg ttttcccagc tcaccatttc cctttaaagg
    31741 atttcttcta actgaaaaac aatatttttt tctgggatct tctgaatcac tttgacttaa
    31801 tgagaaacac atggcttctt tttggtcttc atttttcttc tcactaactt ctgtcggaat
    31861 aaatcgagca agagtttggc taaattgaaa taaaacatct cccaagttac tactgtattc
    31921 gataccaaaa tattctctta ttgttttcgt gtctgtatat aattttaccg aattagcata
    31981 gacagctagc atatctgaaa aatcgttttc ctttaaaaat tctagtttta acagagcata
    32041 ttctggaact ttttcctctt tttcaaatag cttaacaagt acaatataat tttgctgttt
    32101 gtaacgaaag taaaaggaat ctatcaccca gcctttatct ttcatgtcta gttttaatag
    32161 tttaagattt tcaaatacat ttaccataat ttatacctct ccgtgctatt tattaattta
    32221 attataacac ggattgttaa acttactgac aatattcaaa taccccatga gaggtaacta
    32281 ggtaattacc ctctatttcc atatcacgtc ccaaagattg gtagtcaata taattttgta
    32341 aattggaagg gacttcacca agctgtcctg tttcttccac gtaataacgg gcgacatctt
    32401 ccatcgaatc acaatcacta taacaatgaa tatcctcttt gttttccagt aattcttcca
    32461 agctactaaa ccacatgcct tgaatttctt tgagttcatt ataaataggt gtaccttcga
    32521 tttcttgaat ggcttcacat agacggttga tttcactaat cggcgtgtat tcgtccacat
    32581 caaaaggaag ctcaaaatca tgtattgcat attcttcgta gtcctcattt aaaccgattc
    32641 tttctgccat ttcttcctca tcaattggtg gagtaaacca tgctccgact aattcacctt
    32701 cattataccg tcctaaattt gccacataga ctttcatttc caattcttat cacatccttt
    32761 ttataaaaat agtcataaaa aaagtagcaa tcagttaatg acgctactta tcaaggtatc
    32821 cgctaatata atctgtaact gtacttgaaa tacctattat tagttttgct tctctctttc
    32881 caataatatt tgtattaggg tgtgcaagtg aatgcctatt tcttgcttca ttaagagagc
    32941 taatcattcc atttgatgac cttatcgttg tcttcactat atcagcaagt tcagtaggtt
    33001 gaatttcttt ttcaattaat tgttgtatcc tactatataa tttagataga ttttcgtctc
    33061 taggaactgt aatttcatat ttttttagta tttcaattaa atatccatga aaagcagtgt
    33121 gaactctgtc aaaggcacgt tgatattcac cattactcat aaaagactca gcgtcttcaa
    33181 tagcttgtga aaccacatca ctaattttca actgactaaa gttaattact tcgtaattct
    33241 ctggaagtgg tactaaaata gtatccacta atacattatc accttgttta tcatatatgg
    33301 agcttgctgt ctgggtaatc aaatctttat gactttcaaa aaagtctaaa tcatcaaaag
    33361 gaactcttat tttaagatat gtgcaaagcg tattccagcc ctttttatat cctttataat
    33421 aagtaaaatc gtcagtctca tctaaagtta cctctgatat tttcatttta gaaacaatct
    33481 cgttacctcc ttcataataa tttgaagagc tctttctttc taatgtttta attaaaagag
    33541 atttaaattg atcatttgta aacatattca taaatccttt cataacaaaa tatatacttc
    33601 ctattttatc atgaaaaata tctgttaagc accaatgatt cggttaaata agtttaataa
    33661 cacatctttc acgccagctg cattgaacac aagtccgacg gcgattagag caataatcaa
    33721 gaagccaatg agttttgaaa attctcgttt aaaacctaaa aacagtccaa tgacaacaat
    33781 cgctaacaat accaatgact gtgcattatc caagaaccag ttgtacaaat tctgcccaaa
    33841 attcatgtga ttgtcctcct ttccttaaga tatttttctc gataatagat atagcttttt
    33901 acacgcctta ataattgaaa aggggttaaa tgctctccat agacggttac tcgattggta
    33961 taattattat aaaatctcac cttgtaaaaa tcataggtat agatggtatc accagcttgc
    34021 cgttttccct caataatgag agacgtatat ttccctgtat tttgtatata cgcttgaaac
    34081 tcaatgccac actgaaaccc taacttcttt aattcttttg ttaaaaggct catgtaatca
    34141 aatcctccat ggctagatgt tgttgttcaa ttaatttctc atgtttttct gtcagtttgg
    34201 cttcctgaat catggtggag ataatggttg taccgttgag aacatctaaa atgcttgcga
    34261 tttttaatgt aggggcgact tgatgatgta accagttcaa tgttcgttca aaagaataag
    34321 gctctggctg tgtggttagg cgtaatgctc cacgattttt accgataaac cattcccaac
    34381 gttcattagt tttccaatca ctccgacgtt tagtgtcgtc tttatctgca aagcggatat
    34441 agcgattgat aatttcaaag gctgtttttt ccgcctgttg atgtgccaat aaatctttca
    34501 tggcttgggt ggctcggtca ttttttaacc gaatttcaaa ccgattcttt acttctgcgt
    34561 cttctaaggg aacatcattt ttcacgtatt gctcataatc tttctcatat atacagaaat
    34621 agacttcact ttttaatgag ccaatataaa gtgtgtttcc catgtatggt ttttcatcac
    34681 ggtgaaccaa ttcaccactg cgataatttt taaagcttcg aaagacggaa atgcattctt
    34741 cctgtttaca tttctttgcc aaatctggaa tattcaaaat ccctgtttta tcgttgatag
    34801 ctaaatccaa gcgtttgaaa atacctttat gttccatgca atcttgaaag aaatcatacc
    34861 agctccgatg ttgagccaat agaaagtttt caaattgccg acagcctttt cctttcagtt
    34921 ctaataaaac acctttactt acatcatgag agactaggac aacaatatca ccgaaacgat
    34981 agtgttcggg ataagaataa tagccaaagt cttcgtgtag catataatcc atattcaaac
    35041 gtaaaacctc accgataatt tgagccacat cattggtagg gaatcgaatc cgtacatagt
    35101 cgaataacat ctccaatggg ttatcgggat tgaagcgttc caaagcgttc agtaaatctt
    35161 ctttcacctg ttgagtaggg actgccttgc ctgtttcaat atccgatagg taaggtctgg
    35221 taatatgaga agcaaccgcc agtttatttt gagaaacgcc ataagccagt cgcttttctt
    35281 tcaattcctt aatccaaatt ttttcgtttt ccattcgcaa acctccaatc taaaaaagat
    35341 gtaaactatg acccttgagt ttacaccttt catattttcc gcaatccttt gtaccatcaa
    35401 cattggttga tgagaattgc ctgttttcag ttgtatttgt gcccccctgt tagatagagg
    35461 gggctaaagg attggcgtgg ctaacgccac accaatcaaa gcaagtctga acctgtacct
    35521 ttcacttcgt gcggtacagg ctcagattgc tttttttgaa tgacttttcc tatttctcct
    35581 aagaaatcgt acctttttgg tacaaggggt gtataaaact cactaatcac actttttcct
    35641 gtatccacgt aacctcttcc cttgatgggt tttaagaaaa attgtttttg tgtatcgctt
    35701 ccgaacatca taccaaagcc catttcagac atacgaccca aagccacacg gaagttaaag
    35761 ttgtcccgaa tcccatcact aaaatatttt gcgtctggac gttgacaggc aagaattaaa
    35821 aagaagcctg cttgtcgtcc taacattaca atttgtttga gtttatgaat aacggtggta
    35881 ctttcacgac ctagcatatc catcattgcc acatattcat caaaaataag aaaattggca
    35941 gacaaaccaa gataagcata attttctcct gttttatagt tagggtgttg tttcatggtt
    36001 tcactgcgat caatcatatc ttcgtaaaat tgattgatac atgccaacat atcttcttta
    36061 cggtaataga cattattcat gaccgcccct aagtctgcca aatcagcatt tttagggtca
    36121 agaatataaa gctttgcatt tgtatgcagg agagattcaa tcaatgtgag aataaaatag
    36181 gattttccac taccagtgcc cccagcaatt aacatgtgag ggagagaatc gaactcccaa
    36241 tagaccgatt ccatcaattt gacacgtcca tcttgagcta ccacgtcttc aatgccaatt
    36301 cgattgccaa tagtatcata aagtaggaca tactctacat aagaatcttt caacgttttt
    36361 tccactaatt cacaatacaa tcctgtttct agtttgtttt ctaggtgcaa aagctgatct
    36421 tgatatttcc ctaaggtgat ttctacatga atatggatta gtccatcttt cattcgataa
    36481 tatattttag gaaaatggcg gattcgttgt ttggttttct tgctgggtaa atctttaaag
    36541 aatccctctg attgagtttc ttcggcttca taccaatgat tttctaaaat catacgagct
    36601 aacttttgac ggtgctctaa ctgttttata gaatctatac gatagtgata ataaagccag
    36661 ctgataagtc cgcatacaaa aagagcagtg agaatactgc tccaaaagta tggcgtatga
    36721 agttgataga aaaatgtatc taactgaact gcttgccagt tcaccgattg taacgctctc
    36781 caatgaaaag ggagtaggac gactagaaaa actaccagca atgaaattag tacaaaatga
    36841 tagaccaagt gcttatcgtt ggctttgatt cgctctcctc tgtcatgaag caatggcata
    36901 aaaatcctcc tttccaccta tcaaaataat tgtgtattgt agatacgagc ctgtttatca
    36961 aaagttggtt gagaaatggc ttcatatttc tgataagcga ttcgcttttc aaagcgtaaa
    37021 tttttttctt tcggataata atgacgtcga tagagagtgt gtcctaacag ttttttcgct
    37081 tcttttgtca acggatagag atagcgaaac atgcgtccat tgattttctc aatccctttg
    37141 tattcgcaaa atgcctggga aagccaatgc tttttctcta cctgttcaaa gcgagcattt
    37201 tcctctaata aaaggcgagc acttcgaggg tgtatctttt cccatgactg cttatcacga
    37261 tagacacttg tcttgaaata cccgcagtaa aagaagttgg acgcttgata gacatagcca
    37321 cattttccct caattccatc tgccagtgta tataaaaaat gacagtccgt atgttctttc
    37381 aaccacttaa ttagagctga aagagcttga ctgccaaaat aattggtttg attcatttct
    37441 ggaagaaaac acatctttcc aatttctaaa taatctgtgg tttgaaggga actgtcggga
    37501 aagagtttgc gtatggtttg taggggttgc gtaccccagc ctaattccac aacgcctaat
    37561 aatttttctt ctgaaaaaat ccctagaaag tatttacaca atctagggag tatctttgaa
    37621 taatggtatt gccgaataaa agaaatggct tcatccttcg gaatttcttt caaaatcaat
    37681 ggtgtgtttg tcataagcaa cacctatttt tcactttcgg acgcttcttt tttatatttc
    37741 tgatgaggtt ttgtctgtcc ttttaataca agatcgtctg ctttcacgta ccaatcaacg
    37801 tctgcccctc gaaaagtagc cgtcgctacg gtatctgcca caggattgat taattccact
    37861 tctgcgttgt agtcaaattc tttcaaagga acagaagcgg ggatactgac ttgtatcatg
    37921 cgtccttgtc cttttgattt taagtcatag gtacgttctt taatttcttc ggaaaccgta
    37981 ccatcttcat tctggatacg aacctcacga cgcaaggcgg aaaatttcaa tgtgccaaat
    38041 gtctggtctt tttcaatcac aataccttct gctaatctca tattttttca cctttccttt
    38101 cttatgcttt caccatatcg tctgcgtgca taatgtaatt ggtaaatcct cgattgccaa
    38161 ttttataacc ttctgcggta atatgtgggt tgatgagttt gactttttcg tcggattcaa
    38221 aatgtttctc gccagcttcg gagggaagaa tgacaacaat atcatctgct cgttgaatcg
    38281 ttgagtataa attgtaacta cgagagagaa tggttggtct gccatttatt cgacgttgtt
    38341 caatgttccc ttcaccagca tattctaatt gtccaaatgt tttttccata ttgggaatga
    38401 catatttaag ttccatattt ttacctatcc tttctgtttt tatgtttgaa tgaatcttat
    38461 ttgtgtgatg gttttaaagg ggggagagcg accttttgat tcttgatttt ttattttcat
    38521 aagttcactt cctttcaaaa ttgggtaaaa aaatagacgc tccgtactga gcgtccgatt
    38581 cctactatat ttaaaacaac gactacctag gtattttaaa tattataatc aaattctatt
    38641 ttatctaata attgagtggg ataaagcaag tctcctttac tcgtaaaata acccacctct
    38701 gagaaaagaa ataaattatt ttttgctctt gaacaaatta cgtatagtag ttttctcgct
    38761 gaactaattc ctttatcaga aacattaaaa atttcattcc aatgaggaat tttcccgttt
    38821 agaagattaa tagcgataac agtatcatac tcatttcctt taactttatg tgctgtagta
    38881 agtgtgatac catctcgaga tttaaacatc ttcttaaaac tagatacact actagataat
    38941 gaattatttt ttatccgatc ttctacttcg ttaataaaaa atagatagtg cttaaaaata
    39001 gacgggtaat ttttctcgtc aatattaaga ttaacaaata tatgtttggt aacaaatttg
    39061 tataaatcaa caccgttgtc gtctccacta tacattcttt tcacactgtt tattagctgt
    39121 aataaatcta agtcaataaa ataatcttta acttctattc caaattcatt ttttaatata
    39181 attaaaatat cgtttgcaat tagctttctt cttcgaacct tttcacctga ttcagtaaag
    39241 tacaaaattg ctaacttgaa aaataaacta tgattattta ccttaattgg tgatatattt
    39301 atagcatcaa aattgcaata cggtaattct ttctttaaat actcacccag tgaaaacaga
    39361 ggatagtgtt gaggagcaag tacacaaata tctttttcat gaatgccgct ttcaatcttt
    39421 tcctttatta tattttttat tatgttgggt aaatcctcct tagtaacttt gcaatcataa
    39481 cttataaaac cacggtcgtt tttattattc cctctgcttt caatctcgta aggacaactc
    39541 tgaaaattag agtagaagtc tactaatctc tgattagacc tgtaacagcc atgtagcgtc
    39601 tttgattgaa aagtgtgact agttagcatt tctagttctt caactgattt agctatacca
    39661 tctaatgatc cataaattgc ttggtcaata tctccaacaa acattgactg aatttgaggg
    39721 ttgttttgaa gtaatttagc tattacattg tattgtaatt ctctagtatc ttgaaattca
    39781 tccacataaa tcgcccttat attactagca atattacgtg caacgttact gtttacagtt
    39841 aaaattttat acgctactag taaaatcata tcaaaatcaa tttctttatt tttttccaac
    39901 atagtgtgat acatttcaga aacttgagta tactgaactt cattaatttt aaaatcatga
    39961 gaaagtgaca aatcaatttt gttcagttca tacgttttga gatttaaatt cagcatatta
    40021 ttaatttggc tgacgtaagc tctttgagta taatcatcaa taattgtaaa tccttttgat
    40081 actcttgaga gattcatttt aaaaggataa ataataaatt caagacaaaa ctgatgtatt
    40141 gtccccaccc aaatattaat atcatcatct atactaagta aacttaggcg ttccttaatt
    40201 tcttcagcag ctctattagt gtatgtgata gcaattattc tttttagtga tccatcgtgt
    40261 ttaattgctt ggtaagcaat tttccttgta agtacccttg ttttcccact cccaggacat
    40321 gcatttacat aaaggttatc ttcactttcc acaacatctt tttgttctaa atttaaatca
    40381 tttacgattt tttcgaagat atttgtcatt ttgaaacctc agcataaagt atttttgtgt
    40441 aattttctat ctgattgatt agatacacaa catcctcatc tttaccaaag tactctaata
    40501 aagaaattat tgctatattc aaatcagctg tatcacttgt ttttaaacta gctgataaag
    40561 ctgacaattc tgtagtttcc tcgtacaagt cgattgtatg gagtagaatt tttttataaa
    40621 ttgagagatc tactatttcc tgacatgcaa aagcaatagc attaagaata tacttaggaa
    40681 ttttgattaa aaagtcaatt ttttcaccta actcaattgc aaaccacccc ttaccaacat
    40741 gattggctag ttttaacatc gctaaatttc tttctaaata ttgttcactt tgtatttctt
    40801 ctttataagc agttattgtt tttttatctt tgaatgcgtc atctataatt ggactgacat
    40861 aagttagaat attttcttta ttcacatcta aaaactcaat ttcaaatgtt gtattagcat
    40921 aaaaacaatt aacccaattg ttttcattaa aaagggagga taatttttct tttcttgctt
    40981 gacctttctc ttgtgcagtg cttttataca atcgatgttc tgatgggatt gcctgttcat
    41041 ctaaatcagt tacaatagca cactttctct gtattctatc atcgtcaaac aagctagcga
    41101 tatattcaaa cgaagtacta ccaacattaa tcaaacctat tccaagttcg tctaagttta
    41161 taccaaataa tttttttact atttgaggaa taagtatttc ttcagcatct ccttcaacta
    41221 agataacacc tttagaaaaa agcaatccat ttcttttaac atctaaatat ctctctactg
    41281 cgtttattag atttagattg ttctttttta acttttcttt agcaaatgag ttcagattat
    41341 ttgctggatt cataacgata gatttgccat taaacgattt taatacattc atccgagata
    41401 tttcagacac ttcagctaaa tgtacagaat gtgtggtcat gagaatctga gtatagtttt
    41461 tttgaagatt taatccttcg aacagagttt tttgaatatg atgatgaata tgagcttctg
    41521 gttcttcaat caacataata ttaagtagtt ctcttgaacg acacgcttca aattcgacta
    41581 ttttcaaagc taaataaatc atatttagat gtcctaaccc tagtaagtct aagtcgatat
    41641 tttgttctgg cttcattgaa agaaattttg ctaaagaacc catatcatct gacatagttg
    41701 aggaaagaag aagattagga gaatacactg aaccaattat gctatttagt tgccgattaa
    41761 tattttcgcc aattttccta atttcaggaa cactggtaat agtctcattt aattgctgta
    41821 taattgtttt taatgactca acattatctg atgatatttt tgattcaatt gtttccatga
    41881 ttcttttgac tgggtttcga ttgtttttca tttctctaag aacatctcga agagcgtcaa
    41941 cataaacaac cgaaatatgc ttgaaaatat ctgacatgtc aattcttgag cccaacagag
    42001 cttcatcgtc ttcttcagga ttactagcta tattgtcatt gagtcttcct accaatttat
    42061 cataattttc attgatacag aaatttagat ttgatttaga tgtgtagtaa aattcatagt
    42121 cggatagacg aatagaattt ctaaattcgt taaattgatg agagtctaat tcagtggaaa
    42181 tatcaaataa ttgctttcta atagctttgt taggtctaat aaatagagaa atactaccta
    42241 cacctttatc tgcatttgaa atcaattctt ctaagattgt ttgactttca gaatttaaag
    42301 aaatggaagc acaaatctca ttgtcaaatt cttcttcgct gattccttca aatgtcgcag
    42361 agataataat ccaatgccct tgccacccat tttgaattcc atgaaaaaaa tctgattctt
    42421 ttagtgtttt actactatag taataactat catctaataa gattctcaat gcagtaagag
    42481 cattggtttt ccctgaatca ttctcaccta taatagtatt tgtctcatta tcgaatcgaa
    42541 agatttcgga ttcataattt ctaaaatttc gaatttgaag agtagatata aacataagca
    42601 gatctcctaa ctatttaatt aatatgatta tatagtaagt ttatcataaa ttatgttagt
    42661 aaaagttctc attataaggg agcgtcaata attttgtgta aatgattctt ccctactgca
    42721 gattgtttct aattctcaat cagttggctt agcatttgtt cgagctcgcc ttcggcctgt
    42781 tggaaacctt tatgactgcg acctaggaat ttttggttgt aagtatcaaa agacgagact
    42841 aagaaacgtt ccatcgactc ttcattttgg aactgttcct tgcggtggct gtatttcttg
    42901 atttgtttat taaaggattc aatcaagttc gtagaataaa tactcttgcg tatggccaag
    42961 gggaaatcat agaaagtgag caagtgatcg ttcgaaagaa tcgattcaac cacttttgga
    43021 tagctggttt tccatttttc cgcaaaagta ccacgtgctt ccaacgccac ctctttagat
    43081 gaggcttgat acaccatttt aaaatcatca cagacttcct tacgatcatc gacacgcact
    43141 ttatgactga tattacggga aacgtgtaca caacaatgtt gaaaacgagc tttgggatag
    43201 aaccgactaa tcgctcctac catccccttt aagccatccg tgatgaacag gaggacattt
    43261 ttcaaaccgc gctcttgaag gtccaataaa atttcctccc aaatcgtgat ggattcagtc
    43321 ggtgcaatcg catagctcaa tacttccttt gatccgtccg ggcgaatgcc aactgcgata
    43381 tgaatagctt ccttggcgac ggttttccgc tttaacggaa tatacgttgc gtccatataa
    43441 atagcagcat aacggtcatg aagctcccgc ccattaaacg ccgttacctc ttctgtaaat
    43501 gattttgtta tattggacat ggtttggggc gtgtagtaat gcccatacat tttctcaatc
    43561 aagtctgcga tttccgacat ggtaatacct tttcggaaga ggtgaatgac ggtctcctct
    43621 aacgtgtcat tcgtccgtct ataagcagga acagtctgtt gcttgaactc accattgcgg
    43681 tcgcgcggaa tctggagatg aagttcccca tactcggtct tgaccgtacg gtcataggag
    43741 ccgttacgag aattacccgt gttaaaacca atgcgatcat acttttcgta atctaggaaa
    43801 gccgttaact ccgttttgag aagcgtattg actgctttct ccaaatggac acggaataat
    43861 tcattcaaat cgcctttgtt cgctagagtc tttagaattt ctgtagtaaa atcgttcata
    43921 gggaagtcct ctcttctgtg aatgtgttgt ggtaactcta ttctacagaa gggacttcct
    43981 tttttctatt tacacaaaat attttacact ctccattata acaattgggt taaaaagaaa
    44041 atagatagta ttctacctca tatttatttg aaagtaatat ataaaagacg ttcaaattcg
    44101 aacgcctctc gttaaccaaa tattggattc agaatctagt tttcatatat aaacagagta
    44161 tgattaggtg caaaaattgc acttttattg cacttttatt tttatttata gtgttttact
    44221 gttctgtata ttgttttaac tcccttgata gtatcgcatt ctgttttata cactttacgt
    44281 tgaaaacaga gtgggaataa agagccaaca taatatggag ggggaatccc tataaatcaa
    44341 cattcttcaa tagctaaaat gcttgatttt cattaaaaat aagatggttc cctaccaaaa
    44401 gagactataa tataagttaa tttgtttcat tcttatcata aaaagaagtt ctcgaaaaac
    44461 gaactgtttt ccgagaactt ttttctatcc atctaagtgc tcaaatcata ttcacgacgg
    44521 aattctaagg ttaccctttc ttaaacaaac tttatgttat ctgcacaact ttttgcaatt
    44581 ttttcattgc ttcttacgaa atcgttgaac tctctttagc ataatacaaa gtgtagatga
    44641 agtctggtgc tttactcgtt ttggtaaaaa tgactggaaa tcgtttctgg ttatttttgg
    44701 caaaggcgag gtgcaggttc gattcctgcc ttctacgcta acgatgtggc tgaagggaac
    44761 ctccgatctt gaatcgaaac gatccgcagt ctattgtttc accaatgaaa caaaatcaac
    44821 gttcgtccaa gcatttcctg aagccttgct ataaacccca atttcacaaa cacctgttgt
    44881 aattggaatg tctttgattg taatttttgt ccattcactt gttttaacaa ccgcagtatt
    44941 tagttccgct ccaccataat ttttgatata taactgatta gtatcaaact ctcccccaga
    45001 cattacatag gcactcaatg tgtaagttcc tttgcttatg ccactaacat tttgataagc
    45061 tgatgcctcg tatgcttgat cctgccaaaa agttagtttg tagtccccaa aaaaagtgcc
    45121 cgcttccgtt ttgatcgtac ccttagcggt tccttgttga aaccaggttt gccaattgga
    45181 aggactggtc gttggtccat cgctttcgaa acttgcattt ttaactagat ttatttgcgc
    45241 ccctgcttgt ttaaacgcac gcaagcttgg caaggcgttt ccagcaaaat caaacatcgc
    45301 ttggttgtcc cattcgtttc ccatagtgga ttgaacactg agataattca tacctgcttg
    45361 tgttgcccaa ctcacatttc cgttgtacca aagaggttcc caatagaaga agcccgcccc
    45421 gcgattattc ggtactgcct gaatccgatc ggcaagatcc agcaaatact ctgcttgtcc
    45481 tgtcggtgtt ggttcatatc cccccgcagc tgcttcggct tcaccaaaag catttggcat
    45541 agtatcggca ttcgctgttg tatgcccata agctgtttca actacgatca cttctttacc
    45601 ataccgttgg ctgatcgcat tcatattggt ttgaagttca ttcattgaac cgtgccaata
    45661 cggataatac gacactccaa ttaaatcaaa tgacacacct cgactcgtga tttcatcaaa
    45721 ccaccaagta aatgctcctg tatcgccacc atcagctaag tggagcatta ctggagttgt
    45781 tttaggttga gtgtctcgaa cagcttggat accagacttc aaaaaagcag ccaatcgatc
    45841 aaattctccc ccgccttctc cccaagactt gccataaggc cagagcattc cgctattcag
    45901 ttcattgccg atctgaacca tatcaggata aacgtccagc tgcttcattt ccgacagaac
    45961 gttctttgta tagtcgtaca atgccgtatt catttcttca aaggtcagac cttgccatgc
    46021 tttaggaaga ttctgtttcc caggatcaac ccaaaaatca ctgtagtgaa aatccagcag
    46081 caccttaagt cccagatttt ttgctcgttt tgcaaggtca atcgtcgttt ccagatcatt
    46141 tgtccctgct ccataacttt ctccattact attataagga tcctgccaca aacgcagtcg
    46201 aacatagttg acaccattat tttttaaaat ctctaaggca tcttttgaaa tatcgtcttc
    46261 ataatatttt gcaccagatt tttccatatc tgacaggatc gaaatatctg ctccgcgaat
    46321 aaattcgctg gcagatgctt caacagtttt tgaaacagcc cccaaagccc ctaacacagc
    46381 tgcaaataaa acagctttac gcaaccaaat tcccattttc attatggctc ctcctttatt
    46441 gaaattggtt cagaataaac tgaacagctt tttcaggatc tctcgttaat ccgatatatt
    46501 ctgctccttt tgtcgcaacc agcttgattc ctaagcgatc ggtgtcttct ttgatatcat
    46561 catagtaaga attgacttgc ggtgctaaga ccaccatatc aaaatcctgc agcatttcgt
    46621 aatgagaacc ataagcaccc gccattgcac tgatcggtgc tttcgattca gatgcacctt
    46681 tttttaaagc attagctaac atcgcacttg tccctgcgcc agcacaaagt accaacacat
    46741 tgatttcttt atttgcatcg ctgataactt gactcgctgc cacaggtact tcctcacgta
    46801 catcggcaga agcaccttcc tcggttgcga tcacctctgc tttttgtgct tctttctcta
    46861 ctaagacagc atcgtatgct ttgcaaaatg gtagatagat caggaagtca acgacgatta
    46921 gaaccgctgc tagaacaaaa gctaagatac ccatgcccgt tcctagaacc aaaccgatcg
    46981 gtgctggagt tgcccaaggc aaaacgtaca taaagctgtt catgcccaat acatcgacaa
    47041 aaaacttgaa caggcaaacg tttacaacag gtgttaacaa aaatggaata aaaaaataag
    47101 ggttcaaaat catgggtgcc gcaaatagca atggttcgtt gacagcgaaa cttactggaa
    47161 taaaggaagc tttcccaact gccttcaact gcttagactt agcaaagagt aggaagataa
    47221 aaggaacaac caatgttgcc ccagttcctc ccatagttcc tacaaaattc cctaaaccca
    47281 ccgtcagtac tttagaagct tgttcaccgg aattgaacaa ttgcagattg gtttccacat
    47341 tggcatagat aattgccgca attgccggct ccacgatcga cggtccatga acaccgacaa
    47401 accaaaacag cgccatcgct ccccagataa tagcgattcc taagtacccg tcagctgctg
    47461 taaatagcgg ctgcagcacc gtaatgattg cttctgcaaa tgagatttgg aagatttgtc
    47521 gaatgcctaa atcaattaaa acggccgcaa atactgaaaa agagaaagga aaaatatctc
    47581 ggaaggtttg cgaaatcgtt ccaggaactt ctttaggcat tttaatggta atatctttca
    47641 gcacacaaaa cttatataaa ttaactgtta aaaatgcagc gacaaaggaa gccagtagtc
    47701 cctttgtccc catatattcc gttgaaaatc caccttcaat cggagagacg cttaacaaca
    47761 ggaaacttac cacagaggcc agcatcacag aggtggtgtt gattactttg tttttcggta
    47821 agcgacgatt catcgaatca gccaagcagc gagcagtggt tgctgaaacg agtaacccta
    47881 caacacccat cgaataatta tagactttcc atagccatgt ggagacagat tcaggccatt
    47941 ggtaaccaaa aatctcagga accgcagcaa tcaaaatgaa aatgcttgaa aataaaatag
    48001 ctggcatcgc tgttaaaaat ccgtcacgaa cggctcctaa ataactatta cgtgagattt
    48061 tttcaaaaaa tggtttccct ttttcgattt gtttaatgat tccatccata gcgttcttct
    48121 cctatccttg attctgtctg tacagtacaa taaagtcttg aatcagatct ctcagcaata
    48181 aagtggtcat taaatgatcc tgcccatgga taaaaatgaa tcccatctcc atctctttcc
    48241 cagatgcttc ttccgctaaa atttttgttt gcgaattatg tgcaagggtt aaattttcgt
    48301 cggcttcttt caacgccgaa tcaacatgtt caaagtttcc ttgtcttact tctttcaata
    48361 atttcaataa tgtactgcga gcgtcgcctg aatatgcaac gatctcaaaa ccaagcattt
    48421 gtaattcctc tttattcatc ttttcttcct ccattatcac ttattaaata atcgttttgg
    48481 tttcactgac caacttgtac cagtaagctg attcttttgg gtaacgcttc tgggtttcaa
    48541 aatcaacata aaacaagcca taccttttgt tatacccatt tgtccatgag aaaagatcca
    48601 taagcgacca caaaaaatac cccttcacat ttaccccggc tgttatcgcc ttgcttaagg
    48661 attccaaata gactctcaaa tagtcgattc tcggctgatc cataataatg ccatcttcaa
    48721 actgatcttt ataccccatc ccgttctcag tgatatagat tttgttgtaa tgtgggtaat
    48781 cgcttttaat tcgcagcaac aagtcatata gaccttctgg ataaatcagc cagtcccaat
    48841 cagttcgagg aattccttct ttataaatac gttctccgat tcccttcact ttataaacag
    48901 aggtcccttt ttcacccgta ccgttatgat ggattgcatt ttctccatcg taagctttga
    48961 caaaatggca ttggtagtga ttgatcccta agtagtcatt acgcgtggat gcttttttca
    49021 attcaacgaa atcttcttca ggaaaatgat agctggcctg atttgcttca catatctcat
    49081 caagagcagt caaggtttca gttgaataat agcctaaata ggtagcatct aataagaaac
    49141 ggatcgacaa tgcatcgtct aaaaaggcag catgcttatc ttccggcgcg tctgttgccg
    49201 catatttcgt ctctaatgag tgaactacac caatttcacc cggaagttcg ttttctttga
    49261 aatagttcac tacacgggca tgagcaacca tcatattatg aagacaggcg acgatctttg
    49321 taaaatcata tttgattcct ggtgggaaaa cacctaacaa atattgattg gttgcaactg
    49381 gatagatttc attaaaggta ctccacacct tgacttcttt gaattcatga aaacaaaaaa
    49441 tggcatagga aacaaatgct tctattgttt ctcgattcaa aaaatctcca tggtcaaaca
    49501 aacgtttagg ggtatcaaaa tgatgcaacg tcacaaacgg cgttacgttt cttttcttac
    49561 attcttcgaa cacacgatga taaaaagcga ttccttctgg atttggtttc ccagctccat
    49621 caggaaaaat gcggctccaa gcaatggata atcttagacc atttacacca aaacgttcac
    49681 acaattcaat gtcctttgca tattgatgat aaaaatcact tgccggatcg ggactgaatc
    49741 gcccttgttc ctctaaaaag tcatcccaag caacagcgcc tttgccacct tccttagtgg
    49801 ctccttcaac ctgataggct gctgtggctc cgccaaaaat aaaatcttct ggtaatttaa
    49861 gcatgtacat ctctcctcaa atctataatc agttttcgct ctttctaact gattgcattg
    49921 taaacgcatc caaaacattt gtcaacaaaa acaaacacaa ataatcataa aaaccagaaa
    49981 acagcaataa aatctattgt aacaaacata aaaaccaccc taaatgttta cttttgttcg
    50041 aattagattg actgttgtcc gtttatatga tagattatcc ttaaattaga ggtgaagaca
    50101 atgttgaaaa aagaaagatt gcttactatc gtagaaatgg tcaataaaaa aggaatcctt
    50161 accgttaatg aaatcatcaa tcaattagat gtttctgata tgaccgttcg cagagattta
    50221 gatgaattag aaaaatcggg caagctgctg cgtgtccatg gaggggctca aagtctttcc
    50281 tacactttag atcaagaact ttctcacact gaaaaagcca ctctgcagat tcaagaaaaa
    50341 aaagaaattg ctgccacagc agcctccttg attcacgagg gagaaacgat tttcttagga
    50401 ccaggtacaa caatcgaact attggcgacg catttactga acaagaaaat tcggatcatt
    50461 acaaataatt atccagtttt tgatattctt gctcaagcag atggaccaga aattctactg
    50521 attggcggtg atttccggaa aaataccggc gcatttgtcg gaccgatcac caacgacaat
    50581 ttaagaagac taaagtttac aaaagcattt atcagttcaa atggcgttca caacgaagcg
    50641 atctctactt acagtaccga agaaggagaa gcacagcaga tcgctctaaa taattcccgt
    50701 aagaaatatc tactcgttga taataaaaaa ttcaatcgag atgattttta tgtgttttat
    50761 aatttgcatg atttcgatct gctcattacg gatcgccaga tctcgcggga ggttcaagag
    50821 cactatgcac aatatgtcga tctgcaaatt gctgattcaa ccgaaaatac cactatagga
    50881 gcgtaactat gaaagggatt atatttgatt tcaacggaac aatgtttttg gactctcatc
    50941 tgcatgaagc tgcttggctg catatgatac atgatcatac aaaagatggt ttatcagacg
    51001 aggatatttt aaaaaatatt catggtcgga cgaatacgga gattttgaaa catttcattt
    51061 cagagcattt gaccgcctct gagatcgccg tattgtcaga agaaaaagaa agctattatc
    51121 gtatgctttg cttgcaaaat gaaaacgagc ttcagttgac aaaagggctt gaagcgactc
    51181 ttgaccagct gactgaccaa cagattccat taacgattgc cacggctact gtcaaagaga
    51241 atgttgcttt ttattttgat attttcgatt tagcccgctg gtttgactgg gacaagattg
    51301 tctatgacga cggctctttc cctgggaaac cgcaaccgga tatttttctt aaagcggcag
    51361 acaaactgag cttagctccg gctgattgtc ttgttatcga agatgcttac tcaggattac
    51421 ttgctgccaa acgtgcgggg atcggaacaa ttattgcaat tgatccattc ggtaaaaaca
    51481 aaacagtatt tgaacaggca cagctaaata aagatggaac catcaaagat tttactggtt
    51541 tttggacaga gcacctgtct gccacatccg ttaattctta aacgattcaa ctaactacca
    51601 aagaaaagca actgaaatgc acacctcctt ttataaaaaa ctgccagtga aacgacatga
    51661 aagatacact gctatatcct tagtgaaata tagcagtgta tctttgactt tttcattctt
    51721 ttaaccatat gacgcgttca tctctatcta taattaacat taaaaatagt gttttcacat
    51781 tatttctcaa accaatctta aaaatcatca ggagtacttt tttgatatac ttttattaat
    51841 gctgggcgta atggttaaaa gatttccact agttcctctc caccaagctg agttgaaaaa
    51901 tgcttatctg taaacaacat agacaaagag gaaataaacg tttccgtatc tttctctgtc
    51961 ccaaggatat taattaattt tcctactagg taagtatcat taaacttaag atctgccttc
    52021 acatcaaaag ttttgataaa agcttttatc atgcgtacaa ttttgtcttt atcgccataa
    52081 ttgatttcac cactcatatc aatatagtca ctaatccctg aagaaataat ggcaactgct
    52141 tcttctttcg ccttatcaga taattcattt aatgactggg attgattgat gacttcttga
    52201 gttttatctg ttacgtaatc tctcattatc ctatcggcat actccgcaaa gtaatcttga
    52261 aaaaattctt ctacagggta accaacttcg ttttcccaac caggagaaat ttcattatca
    52321 gtacttatta aatctattaa aaaccaaccc gttctctgag tgttgattct ataatatttt
    52381 taatttccta tataaatcaa aaatattata gaatcaataa gctaattaag aaacaaactt
    52441 tagaaactct agaagaactt ctgaatttat atagaagtgt gtagctactt actaaatata
    52501 caaaagttaa aataaaatac tctggatact ctttatactg cattaaagtt agtaatatta
    52561 acagcaaaga tatcgaaaaa acaataacat aatatgtgtc aaaatttttc ataataagaa
    52621 ctcccttact gttgcatact aacataatcc aaaaactctt ctacttcagc taaagacatt
    52681 ccactttctt tagtccaaaa aacagttttt tctatcgatc cttctctgta aactgtaaat
    52741 gtcggagtcg agttaattga gtagcgactc ttgaattctt tccactcatt ttcatcttct
    52801 ctaacttttg ctacattcac gtaataaata ttttcttttc ccttttctac cagcatatcg
    52861 aatagcttcc tgcttttgat gatcgatttc ttgtgctaca tctagtaaat tttcatcaat
    52921 ttctatatca taaagtattt cactagtgtc tacatccaca atatatactt tttatcagta
    52981 gcaatttttg agtaatccgt taaaatgatc tcagtctcgt cactttcatc agtataaata
    53041 tacgttaaca atgaactcac cttttcaatc ggtgttagct ctaagcctac tgtatttttt
    53101 actattgagt cattatttac caccataact tcgtactgta tataccatcc tatttggttt
    53161 ccatcaatat cagtaattgg ctcactagct aatggagtct ccggaacaat attttcctga
    53221 tatttcttaa actcttctgt agcacgaaaa aggttatcat gttcctcact tgaaactggt
    53281 tgagtcatag aaaatgttac ttggctaaac agagggaaaa taaacactac aatcaacaaa
    53341 gtataaaaaa aagcccttct ttttcatttt ttatcttcca atcacaaatt gaaaaactta
    53401 ttcctgcaac tttgagtcta gtatcacaat cgactgacta caactattca tttacattct
    53461 actttaagtt cctccttaac cattaatttt attaaagata cttgtaatag tgattctcac
    53521 aaaatttcat gaaaaatact ttattactgt atctaaaatt ccaatacaca acttatcgac
    53581 tattatctga aaaccaaaac ttattattcc ttgatgttat tctagcaata gtaactgctt
    53641 ttttattttt ttgcatattt tttcggcgtt actttttaaa gttaactttt tcgacaaata
    53701 gaaagagata cgaatcactg atttttttga agaaaagaaa tggagtgagc tgaacgttag
    53761 agatattggg agctttcgta actgcttatt aaacgataat ggttatttgc aaccgtattg
    53821 ctcgcttcta catgaaatat ttcatgaaat atttcgcaaa acctttgatt atgtagttac
    53881 gtttccattc ttgactaaaa atgtttcaaa aacgaacaaa ggtgttaaaa ggtaaagtga
    53941 tcaatactac tggtcaaaat aagaggttga aagggaattg aaaaataact acagataata
    54001 cttttataaa cgtacgtgct ttgtggttct gtagttaact atataactgg aattagggaa
    54061 tggtatcaat ttgaaaagaa agattctacc aatttatcat atgttaaaaa tggaaaatca
    54121 acagcagttc tttcggaaac cttacacaaa aaccgattat tgaaaatagc cgaaaaacgt
    54181 ttttcggcta tttttttaat taagtttcct tcttcttacc ttttcccact attccgtttc
    54241 acatctttga tgatcttcag cgcacgtttt cgtgttttga tatttgggct tttcaagcgg
    54301 cggtatgcac ttgagatatc tgttttttcc atatggacga cccactttct tctttgattg
    54361 tagtcatgct aaaaaacaca aatcgaaaca attacgattt gtgttttaaa tggtatcaaa
    54421 agttaaaatg aaagtcaact tgttaaaaca gtcgatcaga ctagctgatt gactcctatc
    54481 tctggaaata aattttcatt attacgtttt agctacctaa aaaaggccct cttgtccgtt
    54541 aactttaata tctattgtta tagctagttt acctattgaa cgtactgtgt gttcgataga
    54601 acac
//
LOCUS       DACCSK010000005        53000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCSK010000005 DACCSK010000000
VERSION     DACCSK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.005"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(165..854)
                     /locus_tag="IVP39_000266"
     CDS             complement(165..854)
                     /locus_tag="IVP39_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585371.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(829..2958)
                     /locus_tag="IVP39_000267"
     CDS             complement(829..2958)
                     /locus_tag="IVP39_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7585372.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     PGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(3006..4343)
                     /locus_tag="IVP39_000268"
     CDS             complement(3006..4343)
                     /locus_tag="IVP39_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7585373.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGLAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(4595..4954)
                     /gene="rplT"
                     /locus_tag="IVP39_000269"
     CDS             complement(4595..4954)
                     /gene="rplT"
                     /locus_tag="IVP39_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7585374.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKDALAK"
     gene            complement(5007..5207)
                     /gene="rpmI"
                     /locus_tag="IVP39_000270"
     CDS             complement(5007..5207)
                     /gene="rpmI"
                     /locus_tag="IVP39_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7585375.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(5279..5803)
                     /gene="infC"
                     /locus_tag="IVP39_000271"
     CDS             complement(5279..5803)
                     /gene="infC"
                     /locus_tag="IVP39_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7585376.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            6123..6755
                     /locus_tag="IVP39_000272"
     CDS             6123..6755
                     /locus_tag="IVP39_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7585377.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(6757..7662)
                     /locus_tag="IVP39_000273"
     CDS             complement(6757..7662)
                     /locus_tag="IVP39_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7585378.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASHILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(7747..8400)
                     /locus_tag="IVP39_000274"
     CDS             complement(7747..8400)
                     /locus_tag="IVP39_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7585379.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            8722..9666
                     /gene="mvk"
                     /locus_tag="IVP39_000275"
     CDS             8722..9666
                     /gene="mvk"
                     /locus_tag="IVP39_000275"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7585380.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            9659..10636
                     /gene="mvaD"
                     /locus_tag="IVP39_000276"
     CDS             9659..10636
                     /gene="mvaD"
                     /locus_tag="IVP39_000276"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7585381.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            10649..11734
                     /locus_tag="IVP39_000277"
     CDS             10649..11734
                     /locus_tag="IVP39_000277"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7585382.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            11731..12795
                     /locus_tag="IVP39_000278"
     CDS             11731..12795
                     /locus_tag="IVP39_000278"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7585383.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLASLGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(13044..13847)
                     /locus_tag="IVP39_000279"
     CDS             complement(13044..13847)
                     /locus_tag="IVP39_000279"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7585384.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGATIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(13867..15156)
                     /gene="kduI"
                     /locus_tag="IVP39_000280"
     CDS             complement(13867..15156)
                     /gene="kduI"
                     /locus_tag="IVP39_000280"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP7585385.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVINIGGPGFIEIDGQKEEMKKQDGYYIGKETRHVVFSSQDAENPAKF
                     YISSVPAHHKYPNVKISIDQVKPMETGEGLTLNERKIYQYIHPNVCESCQLQMGYTIL
                     EPGSAWNTMPCHTHERRMEAYVYFDYANEDTRVFHMMGKPDETKHLVVDNEQAIISPS
                     WSIHSGVGTSNYSFIWAMCGENITYTDMDMVQMDQLK"
     gene            complement(15172..15651)
                     /locus_tag="IVP39_000281"
     CDS             complement(15172..15651)
                     /locus_tag="IVP39_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7585386.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLSGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSIPVSGILIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(15657..16685)
                     /locus_tag="IVP39_000282"
     CDS             complement(15657..16685)
                     /locus_tag="IVP39_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP7585387.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDTFKNDPAYKDLY
                     QKIQEQGA"
     gene            complement(16702..17808)
                     /locus_tag="IVP39_000283"
     CDS             complement(16702..17808)
                     /locus_tag="IVP39_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7585388.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(18037..20634)
                     /gene="adhE"
                     /locus_tag="IVP39_000284"
                     /gene_synonym="adhC"
     CDS             complement(18037..20634)
                     /gene="adhE"
                     /locus_tag="IVP39_000284"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7585389.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIICDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(20939..21319)
                     /gene="yajC"
                     /locus_tag="IVP39_000285"
     CDS             complement(20939..21319)
                     /gene="yajC"
                     /locus_tag="IVP39_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7585390.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            complement(21416..22561)
                     /gene="tgt"
                     /locus_tag="IVP39_000286"
     CDS             complement(21416..22561)
                     /gene="tgt"
                     /locus_tag="IVP39_000286"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7585391.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            22779..23477
                     /locus_tag="IVP39_000287"
     CDS             22779..23477
                     /locus_tag="IVP39_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7585392.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(23645..26284)
                     /locus_tag="IVP39_000288"
     CDS             complement(23645..26284)
                     /locus_tag="IVP39_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7585393.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(26903..27934)
                     /gene="queA"
                     /locus_tag="IVP39_000289"
     CDS             complement(26903..27934)
                     /gene="queA"
                     /locus_tag="IVP39_000289"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7585394.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            28127..29302
                     /locus_tag="IVP39_000290"
     CDS             28127..29302
                     /locus_tag="IVP39_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7585395.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            29306..29947
                     /locus_tag="IVP39_000291"
     CDS             29306..29947
                     /locus_tag="IVP39_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585396.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            29944..30861
                     /locus_tag="IVP39_000292"
     CDS             29944..30861
                     /locus_tag="IVP39_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7585397.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            30858..31529
                     /locus_tag="IVP39_000293"
     CDS             30858..31529
                     /locus_tag="IVP39_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585398.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(32071..32559)
                     /locus_tag="IVP39_000294"
     CDS             complement(32071..32559)
                     /locus_tag="IVP39_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7585399.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPKDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(32760..33026)
                     /locus_tag="IVP39_000295"
     CDS             complement(32760..33026)
                     /locus_tag="IVP39_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7585400.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(33026..33289)
                     /locus_tag="IVP39_000296"
     CDS             complement(33026..33289)
                     /locus_tag="IVP39_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7585401.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            33764..33949
                     /locus_tag="IVP39_000297"
     CDS             33764..33949
                     /locus_tag="IVP39_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7585402.1"
                     /translation="MALSYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(34091..35914)
                     /locus_tag="IVP39_000298"
     CDS             complement(34091..35914)
                     /locus_tag="IVP39_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7585403.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(36511..39216)
                     /locus_tag="IVP39_000299"
     CDS             complement(36511..39216)
                     /locus_tag="IVP39_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7585404.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(39522..40469)
                     /locus_tag="IVP39_000300"
     CDS             complement(39522..40469)
                     /locus_tag="IVP39_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7585405.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYHLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            40659..40928
                     /gene="rpsN"
                     /locus_tag="IVP39_000301"
     CDS             40659..40928
                     /gene="rpsN"
                     /locus_tag="IVP39_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7585406.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(41104..41466)
                     /locus_tag="IVP39_000302"
     CDS             complement(41104..41466)
                     /locus_tag="IVP39_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7585407.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(41477..42598)
                     /locus_tag="IVP39_000303"
     CDS             complement(41477..42598)
                     /locus_tag="IVP39_000303"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7585408.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(42614..42964)
                     /locus_tag="IVP39_000304"
     CDS             complement(42614..42964)
                     /locus_tag="IVP39_000304"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7585409.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(43094..44605)
                     /locus_tag="IVP39_000305"
     CDS             complement(43094..44605)
                     /locus_tag="IVP39_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7585410.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(44927..46279)
                     /locus_tag="IVP39_000306"
     CDS             complement(44927..46279)
                     /locus_tag="IVP39_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7585411.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKDSLSMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(46432..47508)
                     /locus_tag="IVP39_000307"
     CDS             complement(46432..47508)
                     /locus_tag="IVP39_000307"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7585412.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            47704..49449
                     /locus_tag="IVP39_000308"
     CDS             47704..49449
                     /locus_tag="IVP39_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7585413.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENGTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTRASQKTETVHEKEFGSFTFRASTSTEDPVAPYTVNYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTLGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(49698..50165)
                     /locus_tag="IVP39_000309"
     CDS             complement(49698..50165)
                     /locus_tag="IVP39_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7585414.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVIIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAIDQGLPSTEDSR"
     gene            complement(50149..51522)
                     /locus_tag="IVP39_000310"
     CDS             complement(50149..51522)
                     /locus_tag="IVP39_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585415.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKEIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLSWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFILAKEMGEPIHLLLITLNHESVSIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(51845..52333)
                     /locus_tag="IVP39_000311"
     CDS             complement(51845..52333)
                     /locus_tag="IVP39_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7585416.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELVSDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(52355..52957)
                     /locus_tag="IVP39_000312"
     CDS             complement(52355..52957)
                     /locus_tag="IVP39_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7585417.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLLFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        15152 a        11392 c         9788 g        16668 t
ORIGIN      
        1 ccaagaggtc gtgagcctgc cgttcatctc cgagtcacaa ggaacaattt caaaaaacag
       61 cttctcatgt ttgttgaata ttttgacttg tgaccgagga gttgacagga gaacaccgtt
      121 tattcggaaa aaaggactgt gacacgactt ttgtcacagt ctctttattt gtaatctaaa
      181 tcatactccg tacatctttt ttctatttct tttatttctt ctaagatccc ttggctacga
      241 atttcattat atggccaaag taagactgcc tctaatacat aaggcgaaaa atcgctttga
      301 ttcatctctt ttgaaaaact catcgctgct atctcgctca atgcacgaat gtttgattga
      361 tgtgtataaa aaggcaaccg ccacagcaca attttttcgc tttgcgtata aattccctta
      421 acagaagctt cgatgtggtg aaatagttca tgtccaataa tcaaagcttg cgctgtttct
      481 ttagagatcg aagctagtcc tgaacatttt aatttttcta acggctccgt catcaatcgg
      541 atcccttcat cttctgcata agttgcaaaa gtgattcgtt cgtttatttg ctctgattca
      601 caggaaacga ttggaacagc catttgttca caaatttctc ctacatcggc aaaagggtac
      661 ttttttttga tgctacatgc cgtttcgata ccacaatcga ttgattgttg tataagaatt
      721 ttctgcttgg acaaaggaat ctttttccaa aataaatctc gccgaaactg gtagaatccc
      781 caaatttctt cggggatctg ttgtaattcg tttagtaact cttctaactt atgtttgttt
      841 gatatttccg gcaacgacga tcacttcttc ctccggttcc atagtagata cttccagatc
      901 catcaaaagc tgtagttgtt ccagatccgt cgctgtaaaa tcagagactc ttgctcctaa
      961 gcagagataa aattctggtc gggcaatcaa ttcagtttga taaactgcca tatcttccca
     1021 aagttgttcg acagctgtca tataattggc tgcagttgtt ttcatacatg aagcatgtcc
     1081 acgttggacc ttgataaatc ctttttgatc gatgatccga agacttccag cattttttga
     1141 acgattctgt tcgccgcata cataaaagaa aggtgtattt tccaataagc aaacttcatt
     1201 tttatttaat cgcatatcct ctgcagcaag ctctagtgct tcttctgttg tgatctcctt
     1261 ggttagatcg gttgctttca cttccgtaga accagtagcg atagccgtta cttttgaagt
     1321 ctgtggatcg atttctacgt ggacttcgat cgattctggt gtagcgcctg attcaatcgc
     1381 tttattcatc gcttcatttt tcaacgatcg tatatcttct ttacttggcg aagggatgat
     1441 acgttctaca acatctcgga ccattgcaag tgctacacca atagaagaaa tcacttcagc
     1501 gttttcagga atcgaatatt tcacacccat tttattacta aagtaagtaa tcaatgaggc
     1561 agcgccgccc cctacaccta caagagaaat ctgatctttc tccaagtgat acttgtctgc
     1621 caattctaaa atcaccggtt caatcttagc ataggatttt tccatgatct gctctgcgat
     1681 gtcttcaacc gtcgtatgac aatagtctgc taatgcctgt atggcttttc gtgcagatgg
     1741 gacatttcca taagaaaaat gttcttcttg cactaatcca gggacattgg ctgcacatgt
     1801 atttgtaata gtcacttctt ctccgtcttc catcactacc ttgacgtaat cagcagggtc
     1861 tccttcttta ggagagaaaa attcaacctt aggacctttg attttttctg tctccgtaaa
     1921 tactgcataa tctagaccgg caatatgtgc agaacgtgga ccgacatcga tgattcctga
     1981 ctgattagcc cgtaccatcg aaccgcctgc cacacctaga acccgaacat ctaatgaaga
     2041 gatatatgtt gggtggccac ctacaattga ataatcgatt gctggacgtc catttttaat
     2101 gaccccgata ttggttgttg ttccacctac ttcaaaataa acaccattag acgctctcaa
     2161 gtacatcaaa gagcccatga ctgatgcagc aggaccggaa agcatggtta aaacaggtcg
     2221 ttttttcatt tcattgattt ccattactcc gccgtctccg cgcataatca tcaaagaaac
     2281 attgacccct gcttctcgta cggaatcttc tgtagaggtt gccgtatcta gcatttttgg
     2341 taaaattgat gcattgattg ctgctgtccg agttcttctt gtcaatccat ataattttgt
     2401 gatgtcagaa gccatcgttg tagggatcga ttttaccgat gctgcttcat aaacaactcg
     2461 ttctggttcc ccattatcta ctccaaatgc catagaagaa accaagacct ccgctctttc
     2521 tctttctaaa gaagaaatcg tttctgaaac acggtctaca tttaagtgtt tgacaggtaa
     2581 gaatgcattg acaatctcta tttttttctt attccctaaa tcgatatcgt tcaatctagt
     2641 ttgtcgtttt gctaaaaaac cttctaatcc accttttgcc atcccgatta cgccgacttt
     2701 tgctacgtct ccttcaatca gtgcattagt cgcttgtgtg gtagaatgtg caacaaacac
     2761 aacatcttct ggactgatat tattttcccg caaacaattt tgaaaagact gaacgacacc
     2821 tgcggctact ccacggacat catcgtgggt tgttttcact gaagattttc cgatgatttc
     2881 gtgcgtcgca ttatcgattg cgactgcttt cgtatgtgta ccgccaacat caattcccat
     2941 tcgcacaaaa cgtttcattt tcttttcctc cattcatcta atcgttttta tcgacgtttc
     3001 gtttactagg cgttcccgta taataaaaac gtgatcaggc ataagatcgc tccgatgatc
     3061 caaccagttg ggatggattt cttcatatat tctcgagaag ttacttttgt ataacctaat
     3121 ccccaagcaa cccaagattg tgtaatatct aaatgctgag gagcaatcgt tgtgattgca
     3181 aataacggga acaaaaaggc tggcgacatc gttggattaa cagctaacac gacagctaaa
     3241 atagctaagc ctgaaccgac tagattcatt ggaccgcgga aaaatcctag tggtgtcaat
     3301 atagcaaata cgattgccaa gactaatggt gattgcggta tccaatctcc aaaaatagct
     3361 gaaaaataag gagaagcata cgcagctgaa ttattgaaca tcgctaaggt catcaagaaa
     3421 ccaaccattg gtgcaacatc gactgcgcca tctgtaaata atttcgcaaa ctgacgacag
     3481 atttctttat aacctccgcg caattttcca gtaaacagca atgcccagat acctgctaga
     3541 ataaaaccta agatgattgg aagatctaaa agaacgaccc ctaaaactgg taataatacg
     3601 gctaaccatg agatcattgg tgcgtcgcca ccttcagctg gtacgtttgc tgccattgca
     3661 tagcgtttct ttttgttcat tgatatatta gcgaccgtta gtaccacaac taagcttaca
     3721 atcatcccaa tcataccaat ttggaaatag tcattataag tatagctttc agccgctgga
     3781 ttgaaaccag cataaatcgt ttgatattgt ttgaaattga cgatattagc aaagattcca
     3841 gccataatcg agcccataaa tgagaacagt gcaaccggta ctgaaatacc taatgacatc
     3901 aagatcggca acacaataac tgcaatagaa atcactggtc cgattcctgt catcgacatg
     3961 aatataacag cggttacaat acatagtaag ctcatcgtga ttcttggctt gtcccctcca
     4021 agttcaacta cctttcgaat cagggtagct gcgattccgc tatctactag aacacgcccg
     4081 aaaaaggcac caaagaagac atttaccaag atcgatttgg cgtactcagc tggccccgtc
     4141 gtatagatat gcgtcaacac atcaattact ccttgccctt ccatcgccgg gtttggtgaa
     4201 aacgtgtttc cgactagcgc taatcccatc catacagtag ccatgatggc aaagccgacc
     4261 atcaagttgt agccttttac acagtaccag accatgaaaa agaatgagat aactaacaaa
     4321 ataccaataa taatatcact catgttattc ctccgtccat tttgtttttc tgataaacaa
     4381 actaatatat cagttaaact cattctaact tatgcagtta gcgctttcgt taaaggatta
     4441 ccataaatgt tcggatttca attaaaaaac cttaattatg ctattttaga cttatacgtt
     4501 attgtcctgt ttcatttgat ggaggattgt aaataaaaaa agaggaaaga acataacttt
     4561 tcgttatctt ctttcctctt tataattgtt tagcttattt agccaaagcg tcttttgctt
     4621 ggtcagccaa tgcagtaaat gctgctgcat cgttaacagc caagtcagct aacattttac
     4681 ggttgatgtc gatttcagca actttcaaac cgtgcattaa ttttgagtag ctcaagccat
     4741 tcatacgagc tgctgcgttg atacgagcaa tccacaattt acggaagtcg cgtttctttt
     4801 gacgacgatc gcgatatgcg tagttgtatg aattcattac ttgttctttt gctgttttaa
     4861 ataaagtgtg ttttgaaccg tagtaacctt ttgctaattt tagcactctt ttacgacgtt
     4921 tacgagttac tgttccacct ttaacacgtg ccatgtataa ttcctcctaa taaaaataaa
     4981 tcttcagatt ctgttatttg tacttcttat ttcatttttg ctagttgttg gcgaatacgt
     5041 ttgaagtcgc cactagaaac catgcttgct ttacgcaatt gacggcgttg tttttttgtt
     5101 ttgccgtgga aacggtgact tgtaaacgcg cggaagcgtt tcaatccgcc tttaccagta
     5161 cgtttgaaac gttttgctga tccgcggtgt gttttttgtt ttggcatgac tatatttcct
     5221 cctcaaaatc tttcgttatc cgactatcgg ctgtccaaca gccagttagt cattcaaatt
     5281 acttactgtc gtttttcggt gccagcgtca agaacatgct gcgtccgtcc attttcgctt
     5341 tttgttccac tgttgcaata tccgcagttt cttcagctaa gcgattcaag actttttgac
     5401 caatttcttt atgggtaatg gcacggcctt tgaaacggat agaagctttt actttatcgc
     5461 ccttttccaa gaacttacgt gcattacgaa gttttgtatt gaagtcattc aagtcgatcg
     5521 taggacttaa gcgaacttct tttacattga tcactttttg ttttttacgc gcttcgcggt
     5581 ctttcttttg ttgttcgaaa cggtatttac cgtagtccat gattcgcgca actggtggtt
     5641 tcgcaccagg ggcaactaac acaagatcca agtttgcttg ttctgcaata ttcaatgctt
     5701 ctgcttttgt cttaactcct aattgctcac catctgaacc gatcaatcgt aactctcgtg
     5761 cacgaatgcc gtcgttaacc atcatatcct ttgctatggt cattcacctc caaatattat
     5821 gagagaaaga aaagcttacc gaataaacgg caataaaaaa acgagttctc ttacagaacc
     5881 cgcgcgtact tcacctaata gaaccagtga aaactatcta gcccagtgac attatcaact
     5941 aaggcgagaa gcgggtgctt ctgcttttat ttctcaacca tcatagtata gcaaaaacta
     6001 atctttttgt caatgttttt catacagttt gtcttccttt tctgaaaatg attagattag
     6061 cagactttct agcagtgatt cccgcttctt tgctatacta aagaaaaacg aggtgaaccc
     6121 acatgaaaaa aatcgaacct attccacctt ctcttcctct tcttacaaag ggagagtttt
     6181 atttagaaga tgaggagatc ttctctggtc ttctgatcaa cgggggtgac caaagctatc
     6241 tcgatgccaa aaatatggta ttaagagaga gtcatatcca aaaacttacg atgcaaaaaa
     6301 cagttttacg tcattttgaa tgcagtaatg tcatttttga aaaatgtgat ttttccaatc
     6361 tggagtggat tggtggcagt ttccaccaag tcgtgtttca tcaatgtaaa ttaacaggta
     6421 cgaattttgc cgaaagttat cttcgagact gtactttcac tgattgtatt gcttcgatgg
     6481 cttcctttag tagtacaaac ttgaaagctg tctccttcga tcattgtcaa ttagaagaca
     6541 gcgaatttta tgaggtcact tggaaaaacc tttttttgtc tgacaatcag ttaaccggtt
     6601 cgaactggtt tcgaacttct ctgaagggat tagactttac gactaatttc ttcacagcaa
     6661 tcgctttatc tcaagattct ttggcgggac taatcgtcaa ccaagagcaa gcacttgtga
     6721 tcgcccaagc ccttggtttg cagatcaaag actagatcaa cgaatccgcc gccacagtgc
     6781 aataagcatc caaatagata agaccaaact gatgatcaag gcgattcccc atccgaaaac
     6841 ggaatgggta aagggtaact ggagattcat tccgaagaat cctgtcacaa tagttggaac
     6901 ggtcaagatc agcgaccaga ccgtcaaaaa tttcatcgtg tcattcaagt tgttattcag
     6961 caaattatta taagtgccag agagctggtc taaaatatgc gaagctaagt tcgtcatttc
     7021 aactgcttgt cgtgcttcga tcagtgcatc gtccaactct tctttttcct tttctgacaa
     7081 cttgcgataa atagccaatg ccttgatttg ttctaaaagt actacatttt gttttgttcc
     7141 tgtaactaag taaaccaaac cgatctctaa atctgaaagt tgcagcagat tcttgttcgt
     7201 tgttttttct cttaatttca gattaagccg ctgacgttca ctattcactt cctcgattaa
     7261 aggaaaaaag gaatcagaaa tcaaaaacaa tgtacgaaac aataaactgt atacactcat
     7321 gtctggcatt cgttccaaaa gcgatcgaat tagtcgaatc acgtactcgg tattcggcgt
     7381 ggtaaatgta aacaaaccgt cttcttttaa aataaaagcg ataggactag tttcataatg
     7441 attatcgact ttttgctgat gcggaacatt tgatacaagc agtaacgctt catcaaaaga
     7501 atcgtactct acgcgcgccc gttcattttt atctagagaa taagtcagca tctcgtcact
     7561 aatgccatat tcttctttta agtttttaac agctagcaca tcgttttgct gtacattgat
     7621 ccaaaaatta cggtcttgat taaaatagtg tttttcccgc atactctatc ctcctgacat
     7681 ttgtttccag tataaagaaa aaagtcgctc aaataaaggt ttacccttta tttgagcaac
     7741 tgtaaatcaa atggctactt tggtttggcg aatctctgct aatttttctt cgatcatttt
     7801 caagacttct tcttttgctt gaggatcttc cacaaaatta tagcggtctc catcgatttg
     7861 gatttttgga ctttctttgt attcttcata ccattgatcg taacgtctgt tcaattcttt
     7921 gtaataatcg tatagacttg gatcaaagga caattgttca tacgaacgac cacgtttttc
     7981 gatacgctcc aacatcgtat caaaagatac acgaatatga actaataaat ccggatgttt
     8041 tttatgggca gcatagggaa gttcttccat catgttttcc agcagttcat catacacacg
     8101 aacttctgtt tctgttgctc ttcctagatc tgcatttaga tggaaaagca gtgaatcttc
     8161 ataaatcgaa cggtccaata cattattgtc atctttcatt gcttgtttga tgcttgcaaa
     8221 acgtttattc aaaaaataaa tttgcagcaa aaaagcatac tgttcagggt ttgcataaaa
     8281 taagggcaag acttcattat catcaatcga ttcataaaat gcctgactat caaagtgttc
     8341 agccatcatt tctgttaaac ttgattttcc ggcaccaatt gtacctgcta aaacgatcac
     8401 gctcatttac ctccagctca cttcatcact aaatattgtg tttaactcca acctttttaa
     8461 atataccccc aatatctagt agtgtcaatt gtattttttt ctagtcaaag aagaaaaaaa
     8521 gtcaaaaaat tcttaagaaa aaagtgtttt ttctttagaa aacgtattct tagttttact
     8581 tctgagatgg ttttagtaaa ataaaggagg aatgtttttt gagaccctta gtggacaaaa
     8641 aaattcgtga agcagcgcaa ctcgttttgg gcgctttatt ttttgcttaa aaatcacttt
     8701 tacgtgagaa ggagttagat tatggcaaac tatggccaag gagaatcaag cggaaagatc
     8761 atattgatgg gcgagcacgc ggttgtttat ggagaaccag cgattgcctt tcctttctat
     8821 gcaacaaaag tcaccgcatt ccttgaagag ctggatgcaa tggacgatca actggtttct
     8881 tcctactatt caggaaattt agccgaagct cctcatgcat taaaaaatat caaaaaatta
     8941 ttcattcact taaaaaaaca gcatgacatc caaaaaaact tgcaactgac cattgaaagc
     9001 acgattcctg ctgaacgtgg aatgggatca agcgctgcag tcgccacagc agtcactcgt
     9061 gctttttatg attacttagc atttcctttg tctcgtgaaa tactattaga aaatgtccag
     9121 ctttcggaaa aaattgccca cggtaatcct agtggaatcg atgcagccgc tactagcagc
     9181 ttgcagccga tttactttac aaaagggcat cctttcgact acttttcttt gaacatcgat
     9241 gcttttttga ttgtcgctga tacaggaatc aaaggacaaa caagagaagc cgtcaaagat
     9301 gtcgctcacc tctttgaaac ccagcctcat gaaactggac aaatgatcca aaaattagga
     9361 tacttgacga agcaagcaaa acaagcaatc attgaaaatt caccagaaac gttggcacag
     9421 acaatggatg aatcacaatc acttctggaa aagctgacaa tcagcaatga ttttcttaat
     9481 ctattgatcc aaacagcaaa agataccggg gcattgggtg ccaaattaac tggcggtgga
     9541 cgcggtggct gtatgattgc attagcacaa acaaaaacaa aagcccaaga aatcagtcaa
     9601 gcacttgaag atgcgggtgc tgctgaaact tggatacaag gattaggagt acatacctat
     9661 gtttaaaggc aaagcacgcg catataccaa cattgctcta atcaaatatt ggggtaagaa
     9721 aaatgaagaa ctcatccttc caatgaacaa cagcctttcg ttaacattgg atgcatttta
     9781 tacagagaca gaagtcatct tttctgatag ctatatggta gatgaatttt atctagatgg
     9841 caccttgcaa gacgaaaaag caacaaaaaa agtcagtcag tttcttgacc tttttcgtaa
     9901 agaagccggc ctctcactaa aagcttcagt aatcagtcag aatttcgttc ctaccgcagc
     9961 tggattagcc tcctctgcca gcgggctagc tgctttagca ggagcttgca atactgctct
    10021 taagcttgga ttagacgatc tctctctttc aagatttgct cgacgcgggt ctggttcagc
    10081 ttgccgaagt attttcggtg gtttcgtcga atgggaaaaa ggccatgacg acttaagttc
    10141 ttacgctaag ccagtccctt ccgattcttt cgaagacgat ttagcaatgg ttttcgtttt
    10201 gatcaacgac cagaaaaaag aagtgtccag cagaaatggg atgcgtcgga cagtcgaaac
    10261 atccagtttt tatcaaggct ggttagattc cgttgaaggg gatctatatc aattgaaaca
    10321 agcaatcaaa acaaaagatt tccaacttct cggagaaacg atggaaagaa acggactaaa
    10381 aatgcatgga acgactttag ctgcccagcc tccattcact tactggtctc ctgacagctt
    10441 aaaagcgatg gatgcggtgc gacaattgag aaagcaaggc attccatgct actttacgat
    10501 ggatgctggc cctaatgtca aagttttagt ggaaaacagc catttatctg aggtacaaga
    10561 aacatttacc aaattattta gcaaagaaca agtaattact gcccatgctg gtccaggaat
    10621 tgcgattatt gaataaaggg agatttttat gattgaagta tctgcaccag gcaaactcta
    10681 tattgccgga gaatatgcag ttgttgaaac aggccatcca gcagttatcg ctgcagtcga
    10741 tcaattcgtg acagtcactg tagaatctgc acgaaaagtc ggaagtatcc aatctgcaca
    10801 atatagtggg atgcctgtac gttggacaag acgcaacgga gaattagttt tagatattcg
    10861 ggaaaatcct tttcattata tccttgctgc tattcgcttg actgaaaagt atgcacaaga
    10921 aaaaaacatt cttttatcct tttatgatct gaaagtaaca agtgagttag acagttcaaa
    10981 tggccggaaa tatggattgg gttctagcgg tgccgtgacc gttgcaacgg tcaaagcctt
    11041 gaatgttttt tacgcgttga atttatctca gttggagatt ttcaagattg cagcactagc
    11101 caatttagca gttcaagata atggttcctg cggcgacatc gctgctagct gttatggtgg
    11161 ctggatcgct ttctcaacct tcgaccatcc ttggctccaa gaacaagaaa ctcagcattc
    11221 tatcagtgag ttacttgccc tggattggcc aggtctatcc attgagccat tgattgctcc
    11281 tgaagattta cgtttattga ttggttggac gggtagccct gcctctactt ctgatttggt
    11341 cgatcaagtt caccgttcga gagaagataa aatggtggct tatcagcttt tcttaaaaaa
    11401 cagtacagaa tgtgtcaacg aaatgatcaa agggtttaaa gaaaataatg taacattgat
    11461 ccaacagatg attcgaaaaa accgacaatt actgcatgat ttatctgcaa tcactggggt
    11521 cgtcatcgaa acgcctgctt tgaacaaatt gtgtaattta gctgaacagt atgaaggagc
    11581 cgcaaaatct tctggtgcag gtgggggcga ttgcggaatc gtaattgttg accagaaatc
    11641 tggcattctt cctttaatga gtgcatggga aaaagcagaa atcactccac tgccgttaca
    11701 tgtctatagc gatcaaagaa aggaaaaccg atgaatcgaa aagacgaaca tgtttcacta
    11761 gctaaagcct ttcatgataa acaaaaaaac gaatttgact tcgtacggat catccataac
    11821 cctttgccgc aaatagcagt ttctgatgtc gatctaagta cacaggcagt tgggtttaca
    11881 ctaagcagcc cattttatat caatgcgatg actggcggaa gcgaaaaaac aaaaaaaatc
    11941 aaccaagatc ttgcaatcgt cgcaagagaa gcagatttga tgattgccac tggttcagtc
    12001 agcgctgcct tgaaagaccc ttctttagcc gatacttata cgattatgcg acaagaatat
    12061 cctcacggga aaatcattgc caacatcggt gcagggactt ccgtcgaaag agcccaagaa
    12121 gctatccgat tgtttcacgc agatgcctta cagattcatt tgaatgcacc tcaagagttg
    12181 gttatgcctg agggagaccg tgattttacc aattggaaag tacttattca agagactcaa
    12241 acagccattg acgtccccct catcgtaaaa gaagtcggtt ttggcatgac aagagaaaca
    12301 ctcaatgatc tagcttcttt aggagttcat acagttgata tcagcggtcg aagcggaaca
    12361 agcttcaccc agatcgaaaa cgcccgtcgc tccaagcggg aactgagtta tttagctgac
    12421 tgggggcaat cgacagtctc ttcattgctt gaagcaaatg aagcagacac ttctatggaa
    12481 atcctagcct ccggaggtat ccgcaatgcc tatgacatat ttaaagccct ttgtctcggc
    12541 gcaaatgcag tgggtacttc aggaaccgta ctgacgcatc tgatgaatca tggcgtagaa
    12601 gaaactatta tactgatgaa gcaatggcaa gaagagcttc gtctgcttta tactatggtc
    12661 ggcgcaacga ataccgcggc cctacaccaa caatctctga tcttttccgg accagtgaag
    12721 gattggtgcg aagcaagagg gatcgactta gtgaagtatg gaagacgcac agaaaagagc
    12781 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtacaa acggcggaag
    12841 caaaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
    12901 gaattccttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttcgaataaa
    12961 tgatgtttaa tcagctgtcc ttcggtattc gttcaatccg ctgatttcac aacctctcca
    13021 ctaaacagtg tactaaagct cttttaaggt tgttttccga tataggcaag tatgcctccg
    13081 tccacataca agacatggcc gttaacgaaa tcagatgcgc tagaagccaa gaagacagat
    13141 ggtccagcca aatcgattgg ctcgccccag cgtgctgctg gcgtacgtcc aacgataaat
    13201 tgatcgaatg gatgacgttc accgttttct tgtgtttcgc gtaatggagc agtttgcggt
    13261 gtagcaatat aacctggtcc aattccatta cattggatat tgtattgacc gtattcagaa
    13321 gcgatgtttt tcgtcagcat ttttaatccg ccttttgccg cagcataagc actgaccgtt
    13381 tcgcgtccta attcactcat catggagcag atattgatga ttttaccgcc gcctttttcg
    13441 atcatatccg gaataactgc ttttgaaacg atgaatgggg catttaaatc cacgtcgatt
    13501 acttggcgga agtctgccgc atccatttcg atcatcggtg tccgtttgat gatccctgca
    13561 ttgttgacta gaatatcaac tgaacctaca tcttctttga tctgttgaac catcgcttga
    13621 accgcttctt catcggtcac atcgcagaca taaccttttg catcgatgcc tgcttcttta
    13681 taatgcgcta taccttcatc aactgattct tgattcaagt tattaaatac gattgttgct
    13741 cctgctgtag ctaaagagcg tgcaatctca aatccaatac catagacagc accggtgact
    13801 aatgctactt ttccatctaa acgaaacata tccatactaa attccatcat tttctctccc
    13861 ttatgcttat tttaattgat ccatttgcac catatccatg tctgtatagg tgatattttc
    13921 tccgcacatt gcccagatga atgagtaatt gcttgtacct acacctgaat ggatcgacca
    13981 gcttggcgaa ataatggctt gttcattgtc tacaactaaa tgttttgttt catctggttt
    14041 gcccatcatg tggaacacac gtgtatcttc attggcataa tcaaagtaga cataagcttc
    14101 catacgacgt tcatgtgtat gacaaggcat cgtattccaa gcacttcctg gttctaaaat
    14161 cgtataaccc atttgtaatt gacagctttc gcatacattt ggatggatgt attgatagat
    14221 tttacgttca ttcagtgtca ggccttcacc agtttccatt ggttttactt ggtcgatact
    14281 gatttttaca tttggatatt tatgatgagc tggaacagaa ctaatataga atttagctgg
    14341 gttttcagca tcttgagaag aaaatacaac atgtcgtgtt tcttttccga tatagtatcc
    14401 gtcttgtttt ttcatttctt ctttttgccc atcgatctcg atgaatcctg gtccaccgat
    14461 attgatgaca atcaatagaa ggctaggaat cgcctctttt attatttttc cagcatgagc
    14521 catctgtcct ttttcgacaa cagggtattt attcttttta gcaacgatga atgctactag
    14581 catgattcct agtgcccaca aagcccctac gagataacct cccataaata aagccgaaat
    14641 cgaagcacca ccagcaacaa gtgaatacat gataaaagca gtacttggag gaatgaccat
    14701 acctgtcgga gctgacgcga tattgaccgc tgtcgcaaac ttccggtcat atccttcttc
    14761 cacttgctga gggatcaaga ctccgccaat cgcggtagaa gcggcaatgg ctgagctaga
    14821 gatggaacca aataaagcat tccctaacac attcgtatga gccaaagccc ccggtacgcg
    14881 tccgacaaac aacatcgcaa aatccactag tttcttggct atccctccat tattcatgat
    14941 aatacctgaa agaacgaaaa acggaatagc gatgagcgaa aaactgttga tacttcccac
    15001 cattttttga gctgaactaa aagcagaaac atcaaacggt ataactaaca ataaagtcat
    15061 cattgatgag aacgcaatac ttatagcaat cggcatccct aatacaagca acacgacaaa
    15121 tacaaaaaat aacactaatc cggcttctat tgccatttta ttcctccatt tttatatttt
    15181 ttcttgaatt gctggcttgt tgcttaatgt atcgattaga ttgatcagac tatagataat
    15241 gatgagtata cctgagacag gtattgacaa atagacgtac cccatcggaa tgcttaaaga
    15301 aggcgagatc tgtcccattg tcaatgtgac agcattccat ccgccataca ccatgatcat
    15361 cgcagcaaat accagaaata aactttgaac aacgacttct aaaatcttac tttttttacc
    15421 agataatttt tcacttaaaa ccgttacggc taaatgtgct ttttgtccca caacatacgc
    15481 agaagacaac atggcaaacc agacaagtcc gaatcggaca agctcttctg taaccgtatt
    15541 cggatttcct aaaaccgttc tggaaaatac ttgccataaa acgacagcga ccatcccaac
    15601 aagcaaaaca gctcctagga tctctaacac cttatttaaa atcattttca tcacagctac
    15661 gctccttgct cttggatctt ttgataaagg tctttgtagg caggatcgtt tttgaatgta
    15721 tcatgaagcg gtgcgactgc ctctcgaaac ggtgtattat ctacatcatt gaattggact
    15781 ccatgttctt cttgtgctaa agctttttct tcttcaattg cttttgcaaa agcttcaatt
    15841 tcaaaaaccg ttgattcttt cgctgcttct tcaatggctt tttcctgctc atcagttagc
    15901 tgttctttga tggcatcatt catgatgatg atatctggca cacgcgtatg ctcatcatag
    15961 gaatagtatt tagccacacc accatgtcca gctgtataca agacaaattc attgttttct
    16021 gatccgttga tcaaattgga ttgaagagac gtgtacactt catcacttcc cataggtacg
    16081 ggcgaaccgc ctaaaagctc taccattttg atagcggttt cgctttgcat cacacggatc
    16141 ttctttccct tcaaatcttc cggcgtatgg atcggaccgt ctaccatata aaagctgcgc
    16201 tgaccagaat cataataggt caatccggta aatcctaaat ttttggtcga ttggtagatt
    16261 ggctccatta tttcttcatt atccatcacc ttaaagaaat gttcttcatc attgaataga
    16321 taaggaacgg aaaaaatcga ataatcttta gagaaacttt ccaacgctga ggcacttact
    16381 tttgtgaaat ctactgctcc tgactgtgtc aattcgatca gttcacgttc tccacccagt
    16441 tgtccatctg ggaaatactg gatctgtacg gaaccatctg ttttttcttt tactaaacgc
    16501 ccaaattcat tcattgcatc aattactggc tggctgttgc ttgcataagc aaagcgcaag
    16561 gtaactatct catttacgtc tgcatctgcg acagttgcca cactagaatt atttccgcag
    16621 ccgcttagac ctagcagacc taggctaagc gaacttgctg caaacagcag tgatgttttt
    16681 ttcatatggg tttcctttct tttaacaata agagacgagc aattctgtca aacaaagcac
    16741 cgttaacgac tgtccataag gcattgctgt gattccgata ttccgataaa aatctaaact
    16801 gtcccccatt cctgtcccga ttgaaacgtt ttgaacttct cctttttcat cgatttgttc
    16861 aagcaatcct tggatagcac gataagcgac ttctttatat tcttgtggta aataacgttt
    16921 atggaccgct ttcaaaatac cgtaagcaaa tcctgcagtt gcagaagatt ctacgtaaga
    16981 agaaggatca tccaacaacg tatgccataa accgctttca gactgataag tttttaatgc
    17041 acgcacttgt gcttcaagtg tttcgatcaa taatttgcgc aatgcatctt ctttcgataa
    17101 ttctaagatt tcaatgatct ctggaatagc aatcgtcaac cagcagtttc cacgagccca
    17161 aaatgcttcg gcatagttat gatttccttc aaacgtccag ccgtgatacc acaatcctgt
    17221 tcgtttatcc atcaagtatt tgatatggat cataaactgg taacgagctt cttctaaata
    17281 ttctgggcga ttcaacagtt tcccgatttt tgccaaagga agaactgtca tcatcaatgt
    17341 atcgtcccac aactgattct tattttccgg tccatatgtg gcatgctgta acccgtcttc
    17401 atttgtccga ggcatgtcat tcatgaccca ttctgcccat tgttctaaat aaggcaaata
    17461 gcgtgaatct ttcgtgtctt catacaagta agccatagtc aatagcggtg ccattgtatt
    17521 gacgtttttg ggcggagcgc cttcttccat acgatcctca aaccattcat tgaccacttg
    17581 ataagcgcga ggattttttg tcatctggta atttttataa ataccatata gaccgacacc
    17641 ttgtggccaa ttccacactg tccagctctt gtcatctact ttcagtccat caaagtccaa
    17701 caaaaattca ccattatcgt ctttgatcgt tgtcaggttt tcaacgagca ggtcaatttt
    17761 ttctttcact aattctttag gtacaaaaat ctcttcttta ataatcatct ttacgccacc
    17821 tttttaaacg ctttcatttt atatccttaa tatagcagta ataccgtcta ctataaatat
    17881 cgctttttga acaaaaactg tctaaaattg acctaaatgt taaataacaa aagtaacttt
    17941 cagttacttg gaaattaaaa aaagaacttg gagaaacaac gttttccaag ttctttttca
    18001 tctattttct tatctttttt ctacgctttc ttttgcttat ccgttgtaag catcaatgat
    18061 gatctgtttc aattcagaga tcaatggttc tttcggatta gctgtcgtac attggtcttc
    18121 gtaagctaat tcagccatac ggtctacggt tgtatctagt gtttcttgtg tgactccttg
    18181 agcttttaga ctcatgttga tgccgacaga ttttcctaat tcataaacag ccgttgctaa
    18241 agcttcgact aattcctctg ttgtttcgcc tttcagacct aagaattttg caatatctgc
    18301 gtaatctgta tctgcacgga aatagtcata tttagggaac atcgcatgtt tttgtggatc
    18361 ttttgcatta tagcggataa tatgcggtaa aagaatcgca tttgtccgtc catgagggat
    18421 cccgtattcc ccgccgattt tatgtgcgac tgagtgacaa atacctagga atgcgttagc
    18481 aaacgccata ccagccattg tagaagcgtt gtgcattttt tcacgtgatt ccatatcagg
    18541 tgtttttact gatttttcta aatagtcaaa gactaacttg attgcttgta ggctcaatcc
    18601 acgtgtataa tctgaagcca tgactgaaac ataggattcg attgcatgtg ttaacacatc
    18661 catcccggta tctgctgtga cagatgctgg aactgacatg acgaattgag gatcgacgat
    18721 tgcgacatct ggcgtcaatg cataatctgc taacggatat ttcacatgtg tttcactgtc
    18781 agtgatgacc gcaaatggcg ttacttcaga accagtccct gatgttgtcg ggatacatac
    18841 gaattgtgtt ttttctggtt tgtcgatctt atatgtccgt ttacggatat ccaagaattt
    18901 ttgttttgca ccaaagaaag atgtgtctgg gtgttcatag aacatccaca ttcccttagc
    18961 tgcgtccatt gcagaaccgc ctccaagagc gattactgta tctggcttga agtctgccat
    19021 cagtttcgtt cctgcgtata cggtatttgt cgatgggttc ggttcaacat ctgagaagac
    19081 ttcgatcttc acatcatttt tacgtcgtgc caatacttca cgaactgtat cgcagtaacc
    19141 gaattggacc ataccagggt cacaaatgat catgacacgt tcaacgtttt ccattttctc
    19201 taagtacaat agagagttct tttcaaagaa gatttttggc ggtaatttga accattgcat
    19261 gttatttctc cgtttcgcta cagttttgac attgattaag tttacagcag agacgttacg
    19321 agaaacagag ttcttaccat aagaaccgca tcccaaagtc aatgacggaa tcatttcgtt
    19381 atagatatca ccgatgccgc cttctgcaga tggtgtattc actaaaatac gacaagcttt
    19441 cattcgaaga ccgaatttga catgcaactc ttcgttttct gaatggataa ctgctgtatg
    19501 tcctaatcct ccaagattca acataccttc acacaaatca aacgcatggt ccgtgttgtt
    19561 agctttgatc atggcaagaa ctggagaaag tttttctctt gatagcggat aatctgggcc
    19621 tacgccttcc aattcagcaa tcagcatttt cgttcctttt ggtacactga ttcctgccaa
    19681 ttttgcgatt tccatcgcag aatgtccaac gattgatgga ttgaccgcat attttccttc
    19741 gttcatcact gcatcttcta atttttgcaa ttcatctggt tttacgatat aaacttggtg
    19801 tgcttggaat tctgctttta ctgctgcata gatttcttta tccacgatca cagcttgttc
    19861 agaagcacaa atcattccat tatcaaatgt tttcgaaacg atcaaatcgt tcactgcacg
    19921 tttaattttt gctgtttttt caatataagc tggtacgtta cctggaccta cacctaaagc
    19981 tggttttcct gtagagtaag cagacttgac cattccagct ccacctgtcg ctaagacgat
    20041 cgcgatacct ggatggttca ttagcataga agtcgcttcg atcgaaggat gttcgatcca
    20101 ttggatacaa ttttcaggag ctccggcagc gatagcagca tcacgtacta ctcgtgctgc
    20161 ttctgcagaa gatttttggg cacttggatg gaaagcaaaa acgatcggat ttcttgtttt
    20221 caacgcgatc attgatttga aaatcgttgt ggaagtcgga ttcgttgttg gagttactcc
    20281 gcaaaccaca ccgactggtt cagcaatttc aatcaatcct ttttggacat cttcattgat
    20341 cacaccaact gttttatcat gtttgatatt atgccaaata tattcggacg catacatatt
    20401 tttgattgct ttatcttcgt aaacaccgcg gcctgtttct tccacagcca tcttcgctaa
    20461 tggcatatgt tgatctaaag ctgccattgc catttcatgc acgatatggt ccactttttc
    20521 ttgatcaaag ctttccattt ctttcaatgc tgcatttgct ttttctgcca aatcatcaat
    20581 cattgcttgt acgtcaattg tcttcgtttt ttctttttcc attactttag ccatcattat
    20641 cctcctaatg ggtcatggtt atttgttaaa tcattcacat gtatatcata caatatttgt
    20701 ttttctatgt aaacactttt ttgtgatttt tttcacagtt atttttaaat ataaaattgc
    20761 tttatttaca ggtgtttgta tagaaaaata tataaagtta tttattatta tttatatgac
    20821 acacccgatt attcaatatt gagcaatata tcctatactc ttatctcttt cttttttgaa
    20881 taaaaaatga ggtaggacat atttctgccc tacctcgtct ttaaataagc tttgatactt
    20941 atttttcttt cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa
    21001 ctgtctcagc tggttcttct gttgtttcaa cagtagttgt tgcatcattt gttacgacag
    21061 tccctggttt gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt
    21121 ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga
    21181 tagttacgac tgaatcacct ggtttcatgc tatccaacaa gttttgacgt tcttgctgtt
    21241 gttttttttg cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa
    21301 gcattggtaa tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt
    21361 agcagaaaaa tgctgctaac actagttttt tacttaattt ttaaaagatt ttcctttaga
    21421 agctttttgc gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa
    21481 gcaaattatc atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata
    21541 aattgtggta agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga
    21601 tataggcacg tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga
    21661 aatcatgagc atacttcgca tttttcacta ctagacgacc ttgagaagtc atacatgtgc
    21721 catttcgagc aattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac
    21781 catcgatcaa tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga
    21841 tcaacggtgt agtgaattct aatacacggt tcatttctga tttaggttcc ccgacagata
    21901 acccgccgat tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac
    21961 gaagatcttc aaatcctgct ccttggatga ttccaaataa tccttgacgg tctgggttag
    22021 catgagcttt caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt
    22081 agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca
    22141 atttattttg gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt
    22201 gatttttgaa atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt
    22261 gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc
    22321 cagcttcttc tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa
    22381 taacgccagc acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg
    22441 ttcctactgg catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta
    22501 aacgcgcgcc agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca
    22561 ttcatttccc tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca
    22621 agtgcttttt gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc
    22681 ctttttttat gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat
    22741 ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa
    22801 aacgccaagc aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa
    22861 tccctacatt gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg
    22921 ccaccaccat atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta
    22981 gttcaggagg cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta
    23041 tttcttggac ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag
    23101 atgcatttcg tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga
    23161 tctcgatctt tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct
    23221 attcttattc tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac
    23281 ctaaagtact ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac
    23341 ttttctggtt agatctttca tttatcggct ggcatatctt agcattagca actgctggta
    23401 ttggttatct atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat
    23461 tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc
    23521 gtttatcgag aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac
    23581 attcatgtca cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag
    23641 ttaatcattc ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg
    23701 cgattggtac gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta
    23761 ggccgaatag ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca
    23821 gtaacgccat ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc
    23881 tgctgcgtac attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag
    23941 tttgaccgac atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta
    24001 aataataacc aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca
    24061 ataaacctcg agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt
    24121 gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa
    24181 acagtagttg cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa
    24241 taaagatctc cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat
    24301 aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa
    24361 tcatatctgc ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt
    24421 tacttgcttt caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac
    24481 ccgtacgttg ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga
    24541 cagagagagc tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc
    24601 ctgaaagtgc ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag
    24661 tcaccacatg atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga
    24721 ttgctccttt actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta
    24781 agtctttttc cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac
    24841 caacagcaat cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt
    24901 ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga
    24961 tgtatttttc gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa
    25021 gctcgtttac tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa
    25081 cagcccttac gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt
    25141 gctcgaccgt cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg
    25201 tcattgaatc ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca
    25261 tttcattttg tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg
    25321 tttcaactgc tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt
    25381 atgcaagtgt caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg
    25441 aaatcgaagt catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata
    25501 ctaaagccgc agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga
    25561 cctgtaaagg tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt
    25621 ctgtagccat gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc
    25681 ctttaaatac catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg
    25741 catctttttc aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg
    25801 atgcttcgat caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat
    25861 cacccactac cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg
    25921 ccgtttgttt attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga
    25981 caatcgccaa taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat
    26041 ccccatgttc tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga
    26101 tcgttgtaaa atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct
    26161 catcgaattt attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc
    26221 cagtatctgg atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt
    26281 ccatcctatt cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat
    26341 ccttttttta tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat
    26401 ttaaagtgct tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg
    26461 aggataccat caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg
    26521 ttagaagtac aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg
    26581 cgtatggatg tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt
    26641 gaataacgta aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg
    26701 taacgaaaac aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg
    26761 atggaaagtt acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc
    26821 ttatcgagag ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca
    26881 aaactagcgg cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac
    26941 gatagcgctc ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg
    27001 cactaactaa catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta
    27061 ccactttgaa ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga
    27121 tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag
    27181 ccacgatttt cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca
    27241 aacggtagaa ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga
    27301 atgtacctaa ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct
    27361 catcaagtaa ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt
    27421 ctttcgcata gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag
    27481 gcaatggcat ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt
    27541 caatgatccg tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac
    27601 caaaagagat tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg
    27661 tatctccttg tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt
    27721 ctccatataa acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct
    27781 gtaactcttc aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca
    27841 aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg
    27901 gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc
    27961 ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt
    28021 ttttgttttc ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac
    28081 tagtaataga tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca
    28141 aaatgtgtca aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt
    28201 tgaaaagggt gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat
    28261 gcggatgatc aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc
    28321 catccaagaa attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat
    28381 tggattgatg cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa
    28441 agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc
    28501 ccgtgagatg ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg
    28561 agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc
    28621 gcttgatcca atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt
    28681 gggaaaaacg atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg
    28741 tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa atattttacg
    28801 tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac tacaagccaa
    28861 gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga ttacaccgga
    28921 aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata cgttgttagt
    28981 taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga ataaaaaacg
    29041 aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg taaaagaatc
    29101 cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt
    29161 cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag tggatatcgt
    29221 ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt cagctactcc
    29281 tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg gttcagaaat
    29341 tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca ttggtatcat
    29401 cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca cgattgtgat
    29461 tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat tgatgatccc
    29521 gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt cattattgcc
    29581 gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc gggatgtggc
    29641 aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc ccattgctat
    29701 gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat gggctacact
    29761 tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac tgaataatta
    29821 ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac tattggctga
    29881 ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag aggagaatga
    29941 agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat acttgcgagt
    30001 tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat cacaggtgga
    30061 atcacttcag aagcacagat cttagcaagc ctagttgccg gaatggttga acattataca
    30121 gataaaaata ccgcaatcat caacaatctg gctaccacaa ctatcaacca tcaagcgatg
    30181 atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt aacgactaca
    30241 ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt
    30301 gagaaaagat acgatcaaac gtggttcccg tcctatggtt ttgaaaatac gtatgtcttt
    30361 cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga tctgaaaaat
    30421 gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa aggagacggt
    30481 tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc tatgcagatt
    30541 ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg ctactctacg
    30601 gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca attcttccca
    30661 ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc tcatttaaaa
    30721 gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga attgaactac
    30781 gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt acaagaaaac
    30841 cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa caatttttct
    30901 attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac ttcctgattt
    30961 ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc atgatttcta
    31021 ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag acgatccctt
    31081 ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat gtcgtgattg
    31141 ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc gggatgatcc
    31201 aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct agacaacgac
    31261 tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata agaaacgcgt
    31321 tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt ggtcttggag
    31381 atatcattat ccgaggaacc aatgcaacag atggcacatc gattatttta gcaggtgcat
    31441 tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg gaaagacggc
    31501 tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag cttctgcgac
    31561 catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt ttaaggcaat aatccactct
    31621 gattcttccc tccttatggg caaagagttg ctttttttaa ctatttgtcc attccgtata
    31681 ctccaacttc atactgccaa cgggttcatc tctgcatcca ccgactaagt ttttttctta
    31741 tccgctaagg cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat
    31801 gatctctaaa tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg
    31861 cgtggctttc atttaagcct tttccatcag tcctcgattg ttcgacgcct tcagctgcat
    31921 catcaaaact ggtgactgcc gtatcggtaa cttcgatcat cttttctaca atcgggaact
    31981 tgccatcctc atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg
    32041 tttgcggttc ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt
    32101 ggcgatacga tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg
    32161 ccaataagaa aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat
    32221 actgctattc atggccgaaa gcgccacgac tttgacttct tccgtttgtg tgacattcat
    32281 gttcggcaat gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt
    32341 cgtatctgta tcttttccaa aagaccatgc caacgaatcc tttgggaacc aataagacga
    32401 ctccggttga ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac
    32461 atttccaatt gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac
    32521 taacaacgat gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac
    32581 ataaacccgt caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat
    32641 aggaacaaat taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa
    32701 gagtctcctt tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt
    32761 cactcataat gtgtccaggc agaataaatt tccacaactt tattttcctc atcaactttg
    32821 taaaccactc gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc
    32881 agcttttcaa aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct
    32941 tcaaattgtt tctttaaatt cgattgcttg atttttctta aatccacttt cgcagagttt
    33001 ttgattgcga ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg
    33061 taaagccact gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa
    33121 gaaaaagggt ttcttgaatc gctctccagt cttctagccc aatcaataca gcattgtttc
    33181 cactacgatc actaataatc tcgatttcag tatgattttc atttacctct ttcaataact
    33241 gataaaaatt tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc
    33301 ctttttctga tataattata gcgtacttga ttaagtacgt caaaaaatac aataaagcac
    33361 ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt tttagggagg
    33421 atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa cgtaaagtaa
    33481 ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac
    33541 aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct tgaataacct
    33601 gaggataccc acgaaagatt tgtaacggtt attcaaaact gattttggcc gtcaagtctt
    33661 ctactgcctc aaaaatgatt gcctctttcg tctcctgcca ttcatgttca ctatcgattt
    33721 gatacttgct tagaccgtcg ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg
    33781 taacaaaaaa tggtcacttg acgaggctga gatttctact tatgaagaca catggcactt
    33841 aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat ttggcaaagg
    33901 attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa taggaagaat
    33961 aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc tttagctggg
    34021 aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt ttttgatttt
    34081 aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata ttttcacgcc
    34141 aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc tgccagcgtc
    34201 tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc gctgcatttt
    34261 tacttacgat atctacataa cggataattg gattttcaat cgagcgataa gaagagtgga
    34321 cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt tctttttctt
    34381 tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg
    34441 cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca agaacggtat
    34501 tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct gctacttttt
    34561 tataatgatc atggatctta taaaaaatga acataacgat tggcataatg atgaagaaag
    34621 gccaaatgtc ccctaaccga tagacgaaca aaatcgcaat gattgcatat gagatgaacg
    34681 cacctgtaat attggcaatc gacttgctta accatctttt tccttctttt ttccatttga
    34741 cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag tataacggaa
    34801 tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct agcgctagtg
    34861 tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg tgaggcatga
    34921 atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag
    34981 caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata ccatgtccaa
    35041 agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg atcccatacc
    35101 aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc atcatcgtca
    35161 atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca ttactaatcg
    35221 cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag
    35281 aaacacccgg cacagctgct cctggaagtg cagtagcttg caatggcaca gcacctgtca
    35341 cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat gtgtacaccg
    35401 gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc atcagtagca
    35461 aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc gctgatgtga
    35521 tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg atcaataaag
    35581 agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg acatatgctc
    35641 ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat gaaatcaata
    35701 aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca gacaaagcga
    35761 ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca tcagacgaca
    35821 ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca gattttaaag
    35881 gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta cacacaattt
    35941 tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta cgctcactga
    36001 ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa tatcaagcgc
    36061 tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt tttctttgaa
    36121 ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt tttgatatct
    36181 taacttttga aagagagatg taaggttgct ttctacccat cattcttact actcgaagca
    36241 tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc tgcggtaata
    36301 agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt attacttgat
    36361 gcagaacagc cttttcacaa cctcttggta cacggtgttc aatcagctga ccctcggcat
    36421 tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag
    36481 gtcaaaacgc tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc
    36541 ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc
    36601 aaagacaaca aacagaccga tcatgatcca gattgctttc gtagcatttg gccaataaat
    36661 tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat
    36721 catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga
    36781 cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac
    36841 atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg
    36901 attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc
    36961 aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt
    37021 gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc
    37081 gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat
    37141 tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt
    37201 tgcatctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg
    37261 gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa
    37321 gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa
    37381 tgtaccggta atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa
    37441 tcctaatttt tgctgaagga tcggtaattc attgttgtat aggtctgccc cttcattgat
    37501 gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg
    37561 tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt
    37621 tgctttcgtt gaactgagaa gcgaatcaaa gtcgatctgg tccgctttgc ttagcgcccc
    37681 ttgtgcagtc ccaatcgtat cgatgacttg gtctaataga gaagtgacag tggaactgat
    37741 ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg
    37801 atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga
    37861 taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg
    37921 ctgcaatttc gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc
    37981 gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt
    38041 tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa
    38101 cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt
    38161 gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc
    38221 acttgcctga ttttctaaat ttttcacatc tggcaagata tcctgtactt gttggatgat
    38281 cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa
    38341 atcgccgtcg atctctgcta gttgtttacc ggcattttgg atctctggga ttttttcttg
    38401 caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac
    38461 gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt
    38521 cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc
    38581 gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttgactt tattgatact
    38641 tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact
    38701 ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt
    38761 gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt
    38821 gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc
    38881 tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg
    38941 ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc
    39001 tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt
    39061 atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat
    39121 catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag
    39181 caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc
    39241 agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc
    39301 gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa
    39361 ttaaggatta cagcctctac tttatcatga aataaaaggg aaagataatt ttatgccaaa
    39421 gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac
    39481 aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg
    39541 atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta
    39601 agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg
    39661 attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa
    39721 aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg
    39781 ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg
    39841 attacaaggt cagcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca
    39901 aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag
    39961 attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct
    40021 cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat tagatgataa
    40081 gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt
    40141 tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg
    40201 acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata
    40261 ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct
    40321 cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca
    40381 tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct
    40441 ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct ataagtttac
    40501 gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca
    40561 aaatagttgt cttttttcgc gaaacgtgtt aaattattta tctaaaagcg taaagattac
    40621 gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg
    40681 ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa
    40741 aagcgcaaaa agattacgaa ggcttgcgca aacttccttt agatgctcgt ccagaacggt
    40801 tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt
    40861 cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca
    40921 gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga
    40981 tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta
    41041 tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta
    41101 tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc
    41161 aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc
    41221 gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc
    41281 tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac
    41341 aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt
    41401 tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt
    41461 taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag
    41521 ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt
    41581 ctgcattttt gccgataagc gtcacttttg ttccgacagg gaattcttga ggcaatcgga
    41641 tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata
    41701 gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga
    41761 tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga
    41821 gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt
    41881 ccggtagtgc gtgcccagag ggattcagtc cgtacattgc tactccataa cgaatcatat
    41941 tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag
    42001 gaagttcagg aagttttttt agcacttcta taaagcgttc tttttgcaga ttccaatatg
    42061 tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac
    42121 gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac
    42181 gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct
    42241 ctaaaacagc agctgctgcc tgcaaccatt cccgtgtacc tgcagttact gataaatcat
    42301 gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag
    42361 taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag
    42421 ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgtttgctt
    42481 ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt
    42541 cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg
    42601 tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag
    42661 taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccgtttt
    42721 cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt
    42781 tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag
    42841 gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt
    42901 tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta
    42961 tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa attataacca
    43021 ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac
    43081 tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt
    43141 gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt
    43201 ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat
    43261 ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca
    43321 ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag
    43381 aatattcttc taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa
    43441 gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg
    43501 ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt
    43561 ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac
    43621 gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag
    43681 tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat
    43741 gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga
    43801 ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt
    43861 ttgtacgtcc gaagacgatt gttagttcag gtgtttgaac atcaaacaag cgagtcatga
    43921 tatcgaactt ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca
    43981 tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct
    44041 tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg
    44101 aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca
    44161 aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttgatgtgg tcaagcatac
    44221 gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc tgtcggccaa
    44281 tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata
    44341 attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt
    44401 cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg
    44461 tacctgtttg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg
    44521 attggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg
    44581 ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt
    44641 agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg
    44701 gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga
    44761 agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct
    44821 ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat
    44881 aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct
    44941 gaagctgttc taccacttca aataagccca tgctgttgct gccttttagc ataatactat
    45001 ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat
    45061 agaacacagg tagttggggg tatttttgat tcaattcttc ttttaatgca tgcatttgtt
    45121 ccccgtacaa gaaaatcatg gcaaatttct caggatcgat atgttcactc atttgcgcat
    45181 gcatgcttaa agaatctttc cctaattcca gcatatctgc taacacagca attctgcgcc
    45241 cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat
    45301 tatagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt
    45361 gagtgataga aacatgagcg agcccttggc gtatctcagg tatggacagt ccaaagaagc
    45421 gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg
    45481 taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg
    45541 catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta
    45601 aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctggt acaagtccgt
    45661 cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg
    45721 cttcaccgat cattgtgatt gctgcagctt ttggttgagc catcagtgaa agttccgtga
    45781 tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt
    45841 gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt
    45901 tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc
    45961 ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca
    46021 tggctttcaa aggatctgct acaggaataa ctggaaaatc ttttggagct tgttcaacag
    46081 gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc
    46141 catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa
    46201 attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt
    46261 cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc
    46321 ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa
    46381 gctagaatga acgcggttac gcaatcactc cagcttcttc tttatttctc tttattcatt
    46441 tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaattgaa tcagttcttc
    46501 aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc
    46561 tgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa
    46621 gaaatcacac cggctcaatc cgcttccgcc taacatcgtg taagctaact tcgcgtactc
    46681 ttgtgctttt tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt
    46741 attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa
    46801 agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc
    46861 ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc
    46921 ttctcggttt tctgcctttg taatgccgac actagaaccc atattcgcag gtttgacaaa
    46981 catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca
    47041 ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata
    47101 cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg
    47161 catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa
    47221 aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt
    47281 aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc
    47341 aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag
    47401 ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga
    47461 cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc
    47521 ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg
    47581 aataacttat ttataaaaaa acgagttggt ttctaaaaga acaaacgtca tttatttttt
    47641 gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg
    47701 atgatggcgc aaacaaatcc acgtagccgc ttggcagaaa tcattcgagt gttcatcaat
    47761 tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt
    47821 gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg
    47881 ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca
    47941 ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc
    48001 gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag
    48061 ctgaagaacg gtgactcggt cgtcgttaag atccaacacc ctaacatcgc cgaagagatt
    48121 cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact
    48181 agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg
    48241 gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa
    48301 gagatccgca gccccaaaat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa
    48361 gaaatgtctg gtaaaaactt gaatcatttg atgaatgccg aaaacggaac agaaactttt
    48421 atcaccggta ttcaaaacaa acagctgaaa caagaagttg caaagctttt agttgagaac
    48481 tttatgaaac aggtatttga tgatggtttt ttccatgctg atcctcatcc agggaatctg
    48541 ctattccatg tactaacaaa agaagaacag acacgagcta gccaaaaaac tgaaacagtc
    48601 catgaaaaag agttcggttc ctttactttt cgagcctcta cttctacaga agaccctgtt
    48661 gcgccatata ccgtcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt
    48721 aggcaaaaac tcacacaagc cgttttagcg ctgtatacaa aagatgctta tcgaatcgaa
    48781 aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa
    48841 gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa
    48901 gaagtattca cacacgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat
    48961 atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt ccttgaccct
    49021 gaagtttccc tcatggaagt cgcttcgcct tttgcccaac attattttct cactcatctg
    49081 gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg
    49141 cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggactttggg acaaggaaaa
    49201 gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat
    49261 cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa
    49321 gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt
    49381 gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg
    49441 aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga agggattttg
    49501 cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct
    49561 gaagcaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca
    49621 aaaaggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca
    49681 taaataaaat accttcgtta cctactatct tctgtacttg gtaaaccttg gtcaattgcc
    49741 ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt
    49801 gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg
    49861 atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat
    49921 acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg
    49981 acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta
    50041 gcgtattctt tggtcgtata atggtccaca ctggttacga taatcacgtc gagaccatag
    50101 gattctccta attttatgac atcttctttt actggagaac catctccatc aatgaatagc
    50161 ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaagag
    50221 accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg acactgattc
    50281 atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg ctaaaataaa
    50341 caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag tagtatactt
    50401 gacaacgatc cggcgatcca ttggaatagc tgcatcagtt tgataatatt ccttatttag
    50461 ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt
    50521 tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga
    50581 ccatgataaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc
    50641 ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa
    50701 actgctaaac aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag
    50761 ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa
    50821 cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc
    50881 atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc
    50941 taccagattt cttttgatct ctttgaaatc cagttcgtaa cgcggataat gactaaccgt
    51001 atggtaggcc ataacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg
    51061 tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt
    51121 tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc
    51181 aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa
    51241 ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat
    51301 cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat
    51361 ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag
    51421 tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt
    51481 ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctcccttaaa
    51541 cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta
    51601 cagaatatat tcttgtaaaa aaaaatgcag aaagaagcat aagcttcttt ctgcattttt
    51661 acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc
    51721 tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg
    51781 agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct
    51841 atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat
    51901 ctgaaacaag ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat
    51961 gatttccctg gttcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt
    52021 cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata
    52081 atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg accgtctttt
    52141 cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt
    52201 ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaag
    52261 cttccggcca tacttcttgt atgactgcca tcagctcatc caattcattt tctgttactt
    52321 ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga
    52381 ataaaaagag catgatcatg ctatacacac ctaccgacca tggtttcaag aaaatcacaa
    52441 tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt
    52501 ctggcgcaaa gggaagcagc aaggtaacga ctagtacttt tttgatatct ttcgtttttc
    52561 ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg
    52621 ctacgccata gtaacggaca agtaaaaatc cgattgcttc cccaatcact aggccaatac
    52681 aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc
    52741 ctcccggcaa aataggaatg atcacttgga tcacttgcag cagaacaaat atcatcactg
    52801 catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat
    52861 gatgataagc ataaagaatc aacacaaaga aaaacaacag accaataatg ggcatgatct
    52921 gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc
    52981 atttattaaa actgcctcct
//
LOCUS       DACCSK010000006        49660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCSK010000006 DACCSK010000000
VERSION     DACCSK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.006"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(70..1656)
                     /locus_tag="IVP39_000313"
     CDS             complement(70..1656)
                     /locus_tag="IVP39_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7585418.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            1907..2131
                     /locus_tag="IVP39_000314"
     CDS             1907..2131
                     /locus_tag="IVP39_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7585419.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2273..4024
                     /locus_tag="IVP39_000315"
     CDS             2273..4024
                     /locus_tag="IVP39_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7585420.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            4024..5808
                     /locus_tag="IVP39_000316"
     CDS             4024..5808
                     /locus_tag="IVP39_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7585421.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6265..7116
                     /locus_tag="IVP39_000317"
     CDS             6265..7116
                     /locus_tag="IVP39_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7585422.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            7212..9098
                     /locus_tag="IVP39_000318"
     CDS             7212..9098
                     /locus_tag="IVP39_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7585423.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9139..10041)
                     /locus_tag="IVP39_000319"
     CDS             complement(9139..10041)
                     /locus_tag="IVP39_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7585424.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            10271..11674
                     /locus_tag="IVP39_000320"
     CDS             10271..11674
                     /locus_tag="IVP39_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7585425.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            11674..13107
                     /gene="ascB"
                     /locus_tag="IVP39_000321"
     CDS             11674..13107
                     /gene="ascB"
                     /locus_tag="IVP39_000321"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7585426.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            13252..14178
                     /gene="trxB"
                     /locus_tag="IVP39_000322"
     CDS             13252..14178
                     /gene="trxB"
                     /locus_tag="IVP39_000322"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7585427.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            14392..16119
                     /locus_tag="IVP39_000323"
     CDS             14392..16119
                     /locus_tag="IVP39_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7585428.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            16192..16485
                     /locus_tag="IVP39_000324"
     CDS             16192..16485
                     /locus_tag="IVP39_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7585429.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(16580..17440)
                     /locus_tag="IVP39_000325"
     CDS             complement(16580..17440)
                     /locus_tag="IVP39_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7585430.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            17532..17960
                     /locus_tag="IVP39_000326"
     CDS             17532..17960
                     /locus_tag="IVP39_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7585431.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            18122..18298
                     /locus_tag="IVP39_000327"
     CDS             18122..18298
                     /locus_tag="IVP39_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7585432.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            18325..18771
                     /locus_tag="IVP39_000328"
     CDS             18325..18771
                     /locus_tag="IVP39_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7585433.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(18927..20306)
                     /gene="brnQ"
                     /locus_tag="IVP39_000329"
     CDS             complement(18927..20306)
                     /gene="brnQ"
                     /locus_tag="IVP39_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7585434.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            21012..21983
                     /locus_tag="IVP39_000330"
     CDS             21012..21983
                     /locus_tag="IVP39_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7585435.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            22007..24199
                     /locus_tag="IVP39_000331"
     CDS             22007..24199
                     /locus_tag="IVP39_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7585436.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            24228..24704
                     /gene="ybeY"
                     /locus_tag="IVP39_000332"
     CDS             24228..24704
                     /gene="ybeY"
                     /locus_tag="IVP39_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7585437.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            24682..25083
                     /locus_tag="IVP39_000333"
     CDS             24682..25083
                     /locus_tag="IVP39_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7585438.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            25098..25997
                     /locus_tag="IVP39_000334"
     CDS             25098..25997
                     /locus_tag="IVP39_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7585439.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            26140..26952
                     /gene="recO"
                     /locus_tag="IVP39_000335"
     CDS             26140..26952
                     /gene="recO"
                     /locus_tag="IVP39_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7585440.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            27338..28255
                     /gene="glyQ"
                     /locus_tag="IVP39_000336"
     CDS             27338..28255
                     /gene="glyQ"
                     /locus_tag="IVP39_000336"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7585441.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEFESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            28257..30332
                     /locus_tag="IVP39_000337"
     CDS             28257..30332
                     /locus_tag="IVP39_000337"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7585442.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            30722..31501
                     /locus_tag="IVP39_000338"
     CDS             30722..31501
                     /locus_tag="IVP39_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585443.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            31547..32491
                     /locus_tag="IVP39_000339"
     CDS             31547..32491
                     /locus_tag="IVP39_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7585444.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            32507..33286
                     /locus_tag="IVP39_000340"
     CDS             32507..33286
                     /locus_tag="IVP39_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7585445.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISINQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            33298..34005
                     /locus_tag="IVP39_000341"
     CDS             33298..34005
                     /locus_tag="IVP39_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7585446.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGNVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYTEIKE"
     gene            34026..34790
                     /locus_tag="IVP39_000342"
     CDS             34026..34790
                     /locus_tag="IVP39_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7585447.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            34853..35542
                     /locus_tag="IVP39_000343"
     CDS             34853..35542
                     /locus_tag="IVP39_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7585448.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTQIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTKYDLQRLCVKPGCSGLWQVSERNDVSFHKMVELDLEYIGHRSLMYDLRLIIKT
                     FYIIIKPNGAY"
     gene            35878..36195
                     /locus_tag="IVP39_000344"
     CDS             35878..36195
                     /locus_tag="IVP39_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585449.1"
                     /translation="MYTNSIENVCKSKEIILFSVPSVFVRNTAQKVKPYISDDQIIVD
                     VAKGIESDTLMTLTEIIEDELSEHRIHLVVLSGPTHAEEVAMQLPTTIVSASKNQDIA
                     KRV"
     gene            36223..36327
                     /locus_tag="IVP39_000345"
     CDS             36223..36327
                     /locus_tag="IVP39_000345"
                     /inference="COORDINATES: protein motif:HMM:NF019122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585450.1"
                     /translation="MYTNNNVRGIEICGALKNIIALAAGISKGMGYGE"
     gene            36317..36583
                     /locus_tag="IVP39_000346"
     CDS             36317..36583
                     /locus_tag="IVP39_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAP7585451.1"
                     /translation="MVNNAKAALITRGLAELERLVGRIGCSPKTFSGQLIGQGVPTEL
                     AIKQVGMVVEGINALPAAVKLSKQFQVELWQLMQLSMAERSQKN"
     gene            36685..37080
                     /gene="tagD"
                     /locus_tag="IVP39_000347"
     CDS             36685..37080
                     /gene="tagD"
                     /locus_tag="IVP39_000347"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP7585452.1"
                     /translation="MKRVITYGTFDLLHYGHINILRRAKELGDYLVVVLSSDEFNWNE
                     KQKKCYFTYEQRKQLLESIRYVDLVIPETCWEQKLSDVHEYHIDTFVMGDDWEGKFDF
                     LREEGVEVVYLPRTPEISTTQIKKDLNKV"
     gene            37202..37651
                     /locus_tag="IVP39_000348"
     CDS             37202..37651
                     /locus_tag="IVP39_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HAP7585453.1"
                     /translation="MKVCLVGSSGGHLAHLYMLKPFWENTDRIWVTFDKVDAKAILKD
                     ERMIPCYFPTNRSLKALIINSIIAWRTLRKEKPDLIISSGAAVAVPFFYLGKLFGAKT
                     IYIEVFDRIDKPTVTGKLVYPVTDRFIVEWEEMKKVYPKAINLGSLF"
     gene            37686..38195
                     /locus_tag="IVP39_000349"
     CDS             37686..38195
                     /locus_tag="IVP39_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HAP7585454.1"
                     /translation="MVFVTVGTHEQQFNRLLQAVDSWAENREETVVVQTGYSTYNLVH
                     CISEKFFPYNEMMDYVQKARIVICHGGPASFIMPLQYGKVPVVVPRQVKYEEHVDNHQ
                     LDFCKEVEHRMGNIIVVEEISDLTDILDKYSGIINDKDKQLSSNNELFCKNFSIVVDE
                     LLKNRGSQI"
     gene            38197..39174
                     /locus_tag="IVP39_000350"
     CDS             38197..39174
                     /locus_tag="IVP39_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7585455.1"
                     /translation="MFKYDLSLIIPVYNAERYIEDTISAVVNQTIFPNVEVIIVNDGS
                     SDDTESICEKYVKDYENIKLITQNNMGVSEARNSGLRVATGNYVTFLDADDFVEADLY
                     EKELQLIKDNSADIGVVDFKKVHDDGTIVKYRKNYVRTWNGDNSSIIKDFLSGIIGNQ
                     VVDKIFSKAIINGLSFSGKYKIGEDMLFMYQALERAEKVVMDTSIADYHYIVRSDSAM
                     TGKFDEKYFDPVIISEIILDSCAYEKELKHYAQAHLIHEICKSCEYIYRHKAEKMCPD
                     KLKYLRNKLAEYSVLDAKKYLIKKQFLGFLLMRFVPKIYMLAHRMMHIG"
     gene            39186..40181
                     /locus_tag="IVP39_000351"
     CDS             39186..40181
                     /locus_tag="IVP39_000351"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7585456.1"
                     /translation="MQDKNLSIIVPIYNVADYLERCMNSIIAIKNFKYITVILVDDGS
                     TDSSGLIADEYAKRFDNVCVYHKVNGGLSDARNYGLVRADTEYVFFIDSDDMLVPETF
                     DLVISELEQSSCQVLLFDAKVVDEKDGYLETVDGNYYVHGGLRSNCCYTGKDVIELQL
                     TDHNDFVTTVWLGLYNRDFLIQNNLWFEKGLLHEDELWTIKTLLNAREIRYLMESAYL
                     YRQRAGSIMSSNNRNWNKNIQAVIFIYQSLYAYVDWKVSDIEFARMLKGNLTRRYVHA
                     LSKFEVYKYREIYKKIQFRVLYMNCVRPIDKIRVLILKFTPNMFQWITKLIGKSR"
     gene            40398..41615
                     /locus_tag="IVP39_000352"
     CDS             40398..41615
                     /locus_tag="IVP39_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP7585457.1"
                     /translation="MEILYALFTAIFCTGRFWGIDVEWYISVGVRILIIGLFLVFHKF
                     RFKRNYIIEKNLKLATVPIIAIVVYSIAVWLMSGSVPDEDVVRNLFTANTYLVISYLF
                     ASVLYYEFGKNSIVFLMKCGVVSYLLGSILPLIIKMPRAAIPYLFTSHTNDTSLLWLT
                     EVNDLTFAFGIILLYFLFSYHQNHKKKNIIICALMIFWGLKRIEIAAILLCYLFFKVI
                     TEKIVFSQGAKIFLVSVLSVSYLYVMFIHNGQLAMLAEMYNINFMGRLLTYNYVTETY
                     SSFSPGFLGIGFGRIDEILDDLVAENFRINYVPVISLHSDVLRMYIGLGFIAFGIWIF
                     YQCYYRTMKLKKYFGSKTAQIYICLTLYIFILYLTDNTYSYPITGATYFLAILCVSDE
                     NHFNAYLNKERAK"
     gene            41612..42616
                     /locus_tag="IVP39_000353"
     CDS             41612..42616
                     /locus_tag="IVP39_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585458.1"
                     /translation="MTEKLSSVCLISVYFGKFPDWINVWFLSLKHNPTIDFILVTDQN
                     VDTSGYDNLKVINTDLDSFKMLLQSKLGMKNSSYKLCDYKLVWGLALENEISKYDYWG
                     HSDMDVVYGDIRSFLIRYKLCDYDKFGNRGHLTLYRNTKEVAERYRLTGSWFGSYKEI
                     FKSNRIWAFDETPGINAIYNAHNFSHMNSFIYADIDVNYKDLRLVDRNDPQYVNYQNQ
                     VFYWKKGKLLRAYSKDGTMVVDEFMYIHLQKKRMQKPDFNLEEFDGFYILNGYFEPLG
                     SCITLDDVLKRNIRNDIDLPQKMFGGGYKKDLRFYWLGRRYLNMKYDLKQVIKKLLKR
                     "
     gene            43101..44030
                     /locus_tag="IVP39_000354"
     CDS             43101..44030
                     /locus_tag="IVP39_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP7585459.1"
                     /translation="MKIKKIKEYIKKGTIFSLLRYKVSVFLLKKCTHNKNLAVLSRQC
                     IVGEKLKKEFSYVLENKYKDPFEGKSNKVWFCWFQGYDAAPELIKACYHAAKQAMPNK
                     EIIFLTGDNINSYVHLPDYITEKYHSGKIGFAHYSDLIRISLLCEYGGLWIDSTVLTT
                     SPEMAEYITSLPFFVFKQLELTRLDYQPTVASNWLISSYSNNKILLLTRDFLYEYWKK
                     YNYAIDYFIFHIFFRFATEKYREDWENVPTFNNISPHMLQMELNSEYNEERWKQLLLI
                     SGFHKLNHHIAYNRDNTFFEYVLNRYKSFEEES"
     gene            44027..45481
                     /locus_tag="IVP39_000355"
     CDS             44027..45481
                     /locus_tag="IVP39_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP7585460.1"
                     /translation="MKNNQDNLKYQVMGGMFWKLSERILAQGVSFLVSVVLARMLTPD
                     EYGIVAIVLVLISLANVFVTSGFSVALVQNKNSKDIDFSTNFWCSIAVSVCIYFLLFI
                     IAPLIELFYDIKGLTSVIRVFGLIIPLSAFSAIQHAYVERNMLFKKYFFSTLFGTVVS
                     GITGIVMAMYGMGVWALVFQYFTNTAVDIIVLLFTVNWRPKFEFSILSAKHMMSYGWK
                     ILVADFFGTFFEQLRSLIVGKVYSSSDLAFYNKGNQLPTLITTNVSSSIMTVLFPAIA
                     NISDNYERVYQITRRAVQVVAYIMFPLLFGLAAVSDNLVLILFTEKWLPASIFVKILS
                     IGYAISMIGSVSIQSLKAVGRSDVVLRLEFIKKPIYVLLLILGVNINVTAVAVTMAIY
                     NLYGALVNSNQLRKVIGYKFKEQIQDLFPMLLLSTVIFVVVNMLSNIVENHLFTMIIQ
                     IAIGTGLYITMSVLFKIEVFGYLKEMIVLRFKSK"
     gene            45478..46659
                     /locus_tag="IVP39_000356"
     CDS             45478..46659
                     /locus_tag="IVP39_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase"
                     /protein_id="HAP7585461.1"
                     /translation="MKKILKMIKRKLQMFCGKTKIIIATVLYGKNNIEAHKIVVDNFL
                     GKGYGGDCKYIIDELLNRKLSCDIVWLVTDVNASVPDGVRKVQYGTLQSLKEFLSAKI
                     WISNVRNTLKPTRRTGQVYLQIWHGAYPVKYIEGQQEDLLDKDYVKQAKRDGHDTTAI
                     LVNSLYMEEIFRKYFWMNNHVDYLRFGYLRNDYLLKNIDNKRKIEETRRKLGIKNGAY
                     VILYCPTFRDNFSSNGYIQNAKLFIENLERKYGSVVLLVRLHPNDKKNISFFEYNDKV
                     INGSTVSDGLDLVLASDALITDYSSIAFDGAVLNKPVYILEKDIDTYRTTRGLSEQYD
                     LWPFVKVNNEIDLVEAFISFDSNKYKKRLNAYYQSDISYDTGETAQKTVNWMLKQMSI
                     M"
     gene            46957..47244
                     /locus_tag="IVP39_000357"
     CDS             46957..47244
                     /locus_tag="IVP39_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7585462.1"
                     /translation="MDVWLSQIKEPYNFLNLILFGNASPFIGVGHLWYLFALLYAYIG
                     IYALKRITNKDRLKIAIFLSMVLYLIEVVDYYYGFTVPEYLYRNVFLKDDP"
     gene            47247..47642
                     /locus_tag="IVP39_000358"
     CDS             47247..47642
                     /locus_tag="IVP39_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585463.1"
                     /translation="MIGMNIPVFQRNLSYKKLIAIVMAAGIISIAEWCLFQEQLVVYI
                     NSYIFACVLLIIAVKKSPNVNKHNFFVFFGKNCSLTAFIIHYVFIIILRGMKTPRIIK
                     YAIVLVLSTFVSLIFYHIKKLNANEQRNI"
     gene            47785..49652
                     /gene="glmS"
                     /locus_tag="IVP39_000359"
                     /pseudo
     CDS             47785..49652
                     /gene="glmS"
                     /locus_tag="IVP39_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
BASE COUNT        16497 a         8160 c        10525 g        14478 t
ORIGIN      
        1 taaattcctt cttatttctt gaagctgccc acttctgtca caacctcctt tgtgtaaaag
       61 aatcagtttc tattcgattt cttctatcgt ttcttgcaaa cgctgttcat attttttgtt
      121 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
      181 ataagcgatt tgatcagcca atgctagttg agctgaggtg gcttgctcgc cgaagaaact
      241 ttcgatttga cctggtaatc ctcgtaagtt caagtaagtc agactaatca cagagaacca
      301 gccaagtagt tttacaacaa atgaattttt gaatcggctc cccatctccg cttggctatt
      361 ggtcatcatc agtaaaggaa tcatcgagaa tggcaatgca aaagccagaa aaacttggga
      421 ttcattcatc aagttattca aagcggtatg ttcttcgatc gttccttttg tacttgtaaa
      481 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgactagtc ttcttagcca
      541 aatcgggata cgcatatgga tgaagccttc catgatgact tgaccggtca acgtaccagt
      601 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
      661 agattttgcg acagcaatca agataccatt gctcatagct gatggatcag ataaagcttc
      721 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaatacag cgacacccat
      781 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
      841 agaaaagcgt accgcttgtg ctacatcttc ttcatcttgg tgatcgatct tccgagtttg
      901 agaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
      961 agctccagtc agcggagttt caccgccgat gaccggtgag tctgagaatg tatgactgtt
     1021 agggataaag ccggcaaaca aactgtgcca atcagggtta gacaagccaa cttgatagac
     1081 gaaaacgaat aaaatgacga aaatcaagca gacaaccaaa gcttcgattt tacgaaaccc
     1141 gacttttgtc agcaataaga ggacgaaaac atctaacaca gtgataaata cggcaatcac
     1201 taatggaata tcaaaaagca ggtagagggc gattgctgca ccaatgactt ctgcgatgtc
     1261 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     1321 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     1381 atattgtaaa agcatagcaa tcagactaga catcaaaata actgacatca ataaatattg
     1441 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aacccactgc
     1501 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttcccttggg
     1561 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     1621 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     1681 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaatattc ggtatgccta
     1741 attttttttc gtggcgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     1801 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     1861 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     1921 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     1981 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     2041 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     2101 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     2161 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     2221 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     2281 ttttagaaaa ttaggttggt tttttcgaca ggaaaagaaa cattatttaa ttgggatttt
     2341 ctccctattc gcagtggcct tggtacagtt agttcctcca aaggtgattg ggatcatcat
     2401 tgatgagatt gccgataaaa atttatcaat aaagatcatt tcctggtgga tcttattatt
     2461 gatccttgca gctctcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     2521 aagtgcagct cggttggaga aagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     2581 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     2641 tttgaatgca atccaaaatg tagcaggagc tggtatcttg acctttgcag attcagtgat
     2701 cacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     2761 tgcgctgatt cctctgcctt tactagccgt tacttcacga gtgctggggt caaaactcca
     2821 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     2881 tattactgga atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     2941 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     3001 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     3061 tggaaagtat gtcttggaag gcacgatctc gatcgggcag atggtctcat ttattagcta
     3121 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     3181 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     3241 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     3301 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgtga tccaagaagg
     3361 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacgatca tcaaattact
     3421 gatgcgtgaa tacgatgatt atcaaggaaa aatccaattt ggcggacgtt cgatcaaaga
     3481 atattcattg aatgctttat taggatcaat cggctatgtg cctcaagatc acttcttgtt
     3541 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     3601 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     3661 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac aaaggatttc
     3721 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     3781 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     3841 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     3901 tgtcgatcaa gggaaaatca ttgaacgagg aacccatcat gagctgcttg cattaggcgg
     3961 ctggtatgaa cgaatgtggg agaaacagca attagaagcg aagatagaag gaggcgaggc
     4021 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     4081 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     4141 atcttatttg cgtttgctct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     4201 tttatggatg atcatctggc taaacagtcg gctacgacac aagttatttt gttttttgct
     4261 ggtatgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     4321 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
     4381 catacactag gtatgcgtta ttttgatcag acaccggcag gatcgaccgt ttctcgagtg
     4441 acaaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
     4501 atctttgcag tcgtttcttc cttcattgcc atgtttcaaa tcaatggaga aattgccttg
     4561 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
     4621 tctcgtattt accggaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
     4681 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
     4741 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
     4801 attctgttaa gtccgatcat caattttcta tatgcattgg cgattgcgtt ggcgttgact
     4861 ttgtttggga tcgatgcgct gcattctccc gtagaagttg gactgatcta tgcatttacg
     4921 acctatgtcc agggattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
     4981 acagatggaa tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
     5041 ccgcaacaaa atcctggagc agatcaaacg attacttcag gtaagatcga gtttcgcaat
     5101 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
     5161 ccgggagaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
     5221 gtattgatgc ggttttacga attttatgaa ggccagatct tgattgatga tcatgacatt
     5281 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
     5341 ttgttttatg gagatatcgc aggaaatatc cggatgatga atccaactat cacagacgaa
     5401 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
     5461 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagcggaca acgacagctg
     5521 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcgaca
     5581 gcaaatatcg atactgaaac agaaggattg atccaagaag gattgacaaa aatgcgacaa
     5641 ggacggacaa cgattgcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
     5701 ttagttttag acaaaggtca tatagtagaa cgcgggaatc atgaaacact tctagcgcaa
     5761 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agtggttgtg
     5821 acagggctcc gttcagcttt aggtgataag aaaaaaattg taaaaaaggc tttccagatt
     5881 tttgcataaa tttggcttat caccgataag ctgatactgg aacaccgtat attagaagtt
     5941 gggacagtag catagtagca ttttgaccta agcgctagag tgaaaataaa acagagcttc
     6001 ccagattctt catctgtgag cggtagtgtc gtagtcacca gatttaaaaa agattcgaca
     6061 gctagtcaga agtgcctaat agccactaag aaagcgttga caaaagcgct tgctttcgat
     6121 aatataaaga aaacgataca acttggatag tgattgcctg atgcaagcta aacctgatga
     6181 gtctcccatg gttccatgga cggctttttg ggtttttctt tttgttttgg atacagccct
     6241 atatatcatg gaaaggagta aaacatgctg attgagaaag tactgaataa caacgtcgta
     6301 ataaccagag atgaaagaaa tcaggaaatg atcgtgatgg gaaaaggttt ggcattcaaa
     6361 aagaaagtgg gtgacccgat ttttgactca tgcatcgaca aaaaatttca gttgacaaat
     6421 gacggacttt cagaacagtt ccaagaattg gcacaagcga ttcctcttga gtacttagaa
     6481 atttcttgtg aaatcattga atatacaaaa gaggctctac aagtccagtt gaatgattca
     6541 gtgtatattg cattgacgga tcatttgtat acggctatcg aacgtcacaa aatgggaata
     6601 tttgtaccaa atgtattgct gtatgatatc aaacaactat ttcctaaaga gtatgccatc
     6661 ggggaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
     6721 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
     6781 acctacctgt ttactacaac aatacaggat attctaaata tcgtccgcta ttactataag
     6841 attacatttg atgaagattc agtccatttt tttcgattcg tcacacacct acgttttttt
     6901 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgtac
     6961 caagttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
     7021 ttagccgaga tattcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
     7081 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaggagga taaattaaca
     7141 agaacgcatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
     7201 agggaataga aatgggaaaa tatcaagaat tagcgagcaa aattgtagaa aatgtcggcg
     7261 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
     7321 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtgttgtg acggtaatgc
     7381 gagcgggagg acagtaccaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
     7441 ttataagagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
     7501 ttgatatgct tattgatgga atatcgggct gtttccagcc attccttgct attctagctg
     7561 ctggtgggat gattcgtgga ctgacagcat tacttgtctt cttaggcgca tttgaccgcg
     7621 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
     7681 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
     7741 ttggggcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
     7801 caccactgac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
     7861 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
     7921 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
     7981 atttcttcgt tccattcttc accgttctta ttacaatgcc cttaggtttt ttactcgttg
     8041 gaccagcatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
     8101 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattcta atgatgtttg
     8161 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggctggcaaa
     8221 atatcatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt actgcactct
     8281 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
     8341 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
     8401 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
     8461 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggta
     8521 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
     8581 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaacat
     8641 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
     8701 aggatgcagc ttttgcacaa ggaacattag gtcgaggaat cttgatctac cctgaaaaag
     8761 gagaagtacg tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacatgcaa
     8821 tcggaatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
     8881 agttagaagg aaaatatttt aaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
     8941 atctgttgct ccgcttcgac aagaagaaaa tcgaagagga aggatacagt ttagaaacac
     9001 ctgtgatcgt caccaattac acggattatt tggatatttt ggaaaatcaa ggggagacgg
     9061 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
     9121 taggcttaat tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
     9181 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
     9241 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
     9301 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
     9361 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
     9421 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
     9481 ataagaaatc agaatcccgc acatatcact cgtgcaattg tacgcttcat agaacgattc
     9541 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
     9601 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
     9661 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
     9721 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
     9781 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
     9841 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
     9901 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
     9961 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    10021 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaattataac
    10081 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    10141 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    10201 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    10261 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    10321 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    10381 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    10441 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    10501 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    10561 caaagatgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    10621 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    10681 gcgtttatgt ccaaatgctt ggctgatcaa tttcgcgaac ccaagcggga tggtgacaga
    10741 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    10801 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    10861 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    10921 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    10981 tatcccgttt ttcaaagaac aattaaaaca aatgaacatg attccttgtg gctaccaccg
    11041 ctattactac cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    11101 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    11161 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    11221 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    11281 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    11341 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    11401 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    11461 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    11521 aaaacggata atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    11581 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    11641 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    11701 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    11761 gcgaacgtag acgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    11821 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    11881 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    11941 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    12001 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    12061 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    12121 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    12181 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    12241 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    12301 catcatgagt tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag
    12361 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    12421 gatgtttggg cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca
    12481 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcgaa
    12541 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    12601 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    12661 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    12721 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    12781 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    12841 gattatcgaa tcgactatct ggcagcacat atccaagcaa tgaaagatgc agtagaatta
    12901 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    12961 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgagggaaat
    13021 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    13081 aatggagaaa atctttcgaa tgattgatca atgatttata aaagtctatt gtttttcagc
    13141 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    13201 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    13261 gtaatcgtta ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca
    13321 aacttatccg tattgatgat cgaacaaggc gcccctggcg gccaaatgaa caatacggca
    13381 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    13441 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    13501 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    13561 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    13621 ggacgtggcg tttcttactg tgcagtttgt gatggtgcgt ttttccgtaa caaacgtcta
    13681 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    13741 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    13801 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    13861 gggaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgagttt
    13921 gatttggcag cagacggagt attcatctat gttggactgg atccattaac tgcaccattt
    13981 agagaagcag ggttgacgaa cgaagctggc tggattccaa cagacaacga aatgaagacg
    14041 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    14101 actgctgttg gtgatggtgg gatcgctgga caacaagtgt ataaatactt ggaatcttta
    14161 gaagaaacaa ctgcataagt ttaaaaagag tggaagaacg gtttcaattg ttcttttact
    14221 cttttttttc gcgtgaaatc tactgaatcc gatttatgcg aatacttgca accgtgcttg
    14281 ataaaagaat cctttcttga tttaagcgtt ttttggtcct catactttta tatctaataa
    14341 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    14401 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    14461 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    14521 ttcggtactg caggcatgcg gggaatctta gggcctggga tcaatcgaat gaacatctat
    14581 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg cgaagaaaca
    14641 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    14701 gaagcagcca aaacattagc acatcacaat atcccatctt atgtattcga aagtctacgt
    14761 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    14821 actgcatccc acaatccagc agcttacaac ggatataaag tctacggcga agatggggga
    14881 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    14941 ttaaaagttg aagtattatc tgacgaagaa gctgaacata gcggattgat cacgatcatc
    15001 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    15061 ttggtggaaa caatggggaa agatttgaaa cttgtgtaca caccacttca cggaacagga
    15121 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    15181 gaacaagctg tcgcagaccc agatttcact acagtcaaat caccaaatcc agaagaacac
    15241 tctgcttttg agtatgcgat acgacttggc gagaaagaag gcgctgattt gctgattgca
    15301 acagatcctg atgcagaccg tttaggtgca gccgttcgtt tgccaaatgg ctcctatgaa
    15361 gtattgaccg gaaaccaaat cggagcactg tttgctcaat atattttgga agcacaccaa
    15421 tcgcgaggca ctttacctga aaacgcagcg gtattgaaat cgatcgtttc tagcgaatta
    15481 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    15541 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    15601 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgatccaa
    15661 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat
    15721 gacggcttgc aaacgatttt tgaaacctat ggctactacg cagaaaaaac gatttctgtg
    15781 acaatgagcg gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtgaa
    15841 gaagcaccaa cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    15901 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgatgtattg
    15961 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    16021 atcaaatttt acatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    16081 gcttatgaag cgattatcca aaagatgaca gaagaataaa aaaacttgag actgatggaa
    16141 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    16201 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    16261 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    16321 gcagtggaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    16381 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    16441 ctggatattt tgaaccagac actcaaacat gtggaacatc aataacgata ggtgcaaata
    16501 gagacaaact ggactgtgtc aacgaatctt cttcgctgac acagtccagt tttttttaag
    16561 aaaaagacaa gaaaaactgt tagtctgttt cttcggtggt ttcagttcca taataactat
    16621 gatcttctag gtctaaagca ttcaaaataa gcgaagctgt ttcttcaata gataaagaag
    16681 cgacattgat tactacgcag cccagttttt cataaagatc atttgcaaaa tcgagttcag
    16741 ctcgtatttt ttcgatatca gaataagcag tatcaggatt taatccgtaa gagcgcatcc
    16801 gttctttacg gatattgttt aaaatatctg ggttattcgt caatcccacg atttttttag
    16861 gatcaatctc ccaaagctgt ttgggaatat gtgcttgtgg gatcaaagga agattggcaa
    16921 cttttaaatt tttgttagct aaaaacaagc tcaaaggcgt tttggaagtt cgggatacgc
    16981 ctagcaaaac gacatctgct tctagaaacc cacgaggatc tttcccgtca tcgtatttta
    17041 cagcaaattc cattgcttta atccgtttga aatagttctc attcaataaa tgaagggcgc
    17101 caggttgccg aatgggcttg ataccagtta agcgctcgat ttctgatatc ggcggagtca
    17161 tcacatcaaa actaaataat tgatgttctt cacaaaaaga acgcgtcatc tcgatcaatt
    17221 cttgattgat caatgtgtgt aagacaagag ctttttcttc tagtgcatct tgcagtgcct
    17281 gcatcaatgt tttctcatct gtcacaaatg ttctgcggaa taaattgaac tcaacgctag
    17341 gatattgggc catagtggct tgcgctaatt ttgtcgcagt ttctccagca gagtcggaga
    17401 tgacaaaaaa agtaacgatt tctgtagaat cggaagacat ggataaaacc tcacttgcat
    17461 tttttttatg tttattatac catactctag aatgtgtact tcgtaatcat tttgatttgg
    17521 aggaataagt catgaaaaaa cagacattga tcgaacaatc actagaaaca ttgaaaaata
    17581 acggttttaa atatactaaa aaaagagaag cattgttaac ttatttagtg aagcgcaatc
    17641 gctacgtatc cgctaaagaa gtttttgact atatgaatca ggaattcaaa ggagtaagtt
    17701 acgatacgat ttatcgaaat ctccatgatt ttgcagaaat cgatttactg gaagaaaccg
    17761 atttaaatgg agagatgaag tttcgttttc attgctgtca aggggaaatc ggccatcacc
    17821 atcactttat ctgtacggtg tgcggaacaa cgaaagaaat ccatatgtgt ccaatgaatt
    17881 atttcgaaga acagctcggt ggatgtacga tcgaagggca tagattcgag attttcggaa
    17941 gatgtgaaaa ttgtcaataa ggatttaaat aaaataacat ttgataattt taaaaaaaag
    18001 caagatttcc ttgacggtca aaacagcatt agatataatg tttacaggac agtcttgttt
    18061 tttgagatat atttcaataa caattacggt tactttttaa gctcggaggg agggaattaa
    18121 catgtcaaaa acagtcgttc gcaaaaacga atcacttgac gatgctcttc gtcgcttcaa
    18181 acgttccgtg tcaaaagcgg gtactttaca agaatctcgt aaacgtgaat tctacgaaaa
    18241 accaagtgta agacgcaaga aaaaatctga agcagctaga aaacgcaaaa aattctagtt
    18301 ctgtgatgaa ttggagttgg tagtgtgtca ctactaagta gattaaacga tgacatgaaa
    18361 acagcgatga aagcgaaaga taaggaatct ttacaagtca tccgaatgat caagtcatct
    18421 atacaaaacg agcagattaa agaaggtcac gacttgacag aagaagaaga attaacggtg
    18481 ttgtctcgcg aaatgaaaca gcgacgtgat tctttgcatg agtttgaaga agcaggacgt
    18541 gacgatttag ctgaaaaagt aaaaagtgaa attgtgatag tcgaaaaata catgccggaa
    18601 caactttccg acgaagaaat tcgacaactt gttcaagaag caatcacaca aactggtgct
    18661 tcatcaatga aagaatttgg caaagtcatg ggtgcgatca tgcctaaagt caaaggaaaa
    18721 gcagatggaa accaagtaaa tgccattgtg aaagaactat tacaatctta attttatcat
    18781 ttaaactgcg gacaatcatt gtccgcagtt tttttgtgct cgtaaaaaag aaatctaaaa
    18841 aatagctaga cgaaagaata ggattctttc atctagctgg ttttttcctg tttaatattt
    18901 cttatgttaa attgtgtttg atctggttat tcttctggta gttcatttgc ttcttgtttg
    18961 taccctgata ggcgataggc gtaatagaag agtaagctgc cagcgtaacc aagtaacgtt
    19021 gggaccatcc agccaagtcc taatgcagca aaaggaacgt gatgatgata aaattcgagc
    19081 atgcttgcta caactctttg ttgactcact aaagcaggcg aatttgccag catatccaat
    19141 acggcgggaa cagcagcaaa caacatcgtt acttgataga caaatggtgt cttttggaaa
    19201 aattttgctg ttaaggaaag caagatcaac gcaagcgaca atgggtacaa aagcatcaat
    19261 actggtaaag accattggat gatattgtcc aaaccagcat ttgcaacgac aaaagagacg
    19321 aaagtcgtta accgcagcca tgtcatataa ctgactttag ggaacagttt atggaaatct
    19381 tgtgcaaaag aaacaacgag tcccatagcg gtagtgaaga cacctaatgt gaccatcact
    19441 cctaagaagc tagcgccgaa gttacctaaa taaaaattag taatctgaga aagtgcagtt
    19501 ccgccatttt ctgataaaga catcttattc aaactcatcg caccgagaag aacaagtcca
    19561 aaatagatca tgacttctag cgcaacgctc aggctaccag cttttgctgt taattctgtg
    19621 acttttttat ctttgaaacc aaggtcacgg actgcatgga caaaagtgac acttaaggca
    19681 agcaaagcta ctgcatcaac cgtgttataa ccttctagga caccatttgt taaagcgttt
    19741 gtttggtaag tatctgcagc agattgattt agatttccca ttgggtggaa taaagaaacg
    19801 acaaaaatga ttgctaataa tgcaagaaac aaggagttta gatactttcc gacgtatttg
    19861 accaaccgtg tgggatggac agtaaggaaa taagcaagta aaaagaaaag agcagagaat
    19921 aaaaacatac ctaatgaatt atatttttca ggcaaaaatg gtttagcagc catttcaaaa
    19981 gcagtagcag ctgtccgtgg tgtaccgaaa aatggtccga tcgtcaaatg aacaaggatt
    20041 aagaacaagg cagcatacca ttttccgaca ggtttagcta agtcatacag accatcactt
    20101 tttgtcacta ctacagctaa gatagctagt aatgggaaaa gagcgcctga gatggcaaat
    20161 cctacgccag cactgaccca gtttgtgcca gccatttgac ctaagtggac aggaaagatt
    20221 agattacctg acccgaagaa catcccgaag ataagtgaac tgaccatcaa tagttgtttc
    20281 catgaataag tttttgtttc ttccataaat ttttcctcca ataattatca ttttgattga
    20341 cgatataaac gttgcagcaa ttagtaatgc cattaatatc agattccatg aaaaccacca
    20401 ccttaaaata taaaaaaagc ccttttccac ttgggaaaag ggctctagat tctagaacgg
    20461 taccaccttt tttcgcttaa aaaagcttct tcattcacgg acgctttttg ataaagttat
    20521 ccgctagcct gataatggag gcagccacaa tgatctacgt tttcgacatt gactcggaaa
    20581 tgatgttcgt ctattcgatt tattgtcatt tctcacttac atgactcact gggaataaat
    20641 cgttgaatag cctactgttt tcctcgacgc tttgctgtat gtttatttgt tgacgtaata
    20701 atacacctgt tttttttgaa atgcaagaat attttaacta ttttgttttc tttttttgaa
    20761 acaagaaaaa tacctagaga atacctgctg aatgaaacgg aatattttgt tctccacttt
    20821 catataactt acattctcag attcttttta tcaaaacgag acctttagcg aatggaaacg
    20881 gctatttttt aacgtttctt ggtacgtatg gttaaacgac tcttttacaa ccttttctta
    20941 tttgctatat agtttcttct gagtatggta aaataggaaa gaatgaatcg aaaaaaaggg
    21001 gatgtctatt tttgacggaa gagtctagtt ctttagagat tagattgaca gctgctgatg
    21061 atgtcagcat gcttttaggt gcacacgaca agcatataaa actgatcgag gaaacaacgg
    21121 aaacaaccat ccatacacgt ggagaaatca ttcagatcat tggtgaacaa tcccagattt
    21181 ccttggctgc ctcagtcatt cggacattac aggaattgat caaacgagga atccacgtaa
    21241 gtacgcccga tgttgtgacg gcattgaaaa tggggcaaaa aggtacatta gattattttg
    21301 ttgagatgta tgaggaagag atcgtcaaag accgtaatgg aaaacctata cgggtaaaaa
    21361 acagcggtca aaagaaatat gttgaatcgg tagccaaaca tgacatcgta tttggtgtag
    21421 ggccagccgg aacagggaaa acgtttcttg ccgtagtatt agcaattgct gccttaaaaa
    21481 aaggcgaagt gcaaaaaatc attttgacgc ggcccgctgt agaggcaggg gaaaacctag
    21541 gctttctacc aggggatctg aaagaaaaag tggatcctta tctgcggcct gtttatgatg
    21601 ctttatacca aatttttgga ttggatcata cgaaccgatt gatggaacga ggcgtaatcg
    21661 aaatcgcacc attagcttac atgcgggggc ggacattgga tgatgcattc gttatcttgg
    21721 acgaagcaca aaacacgaca gtcgctcaga tgaaaatgtt cttgacccgt ctcggttatc
    21781 aatcaaaaat gattgtcaat ggggatacaa gtcagataga cttgcctcga gggacgacaa
    21841 gtggtcttgt tcatgcagaa cgaacactaa agcaaatcaa aaaaatcgat tttgttaatt
    21901 ttgaagcaag tgacgttgtt cggcaccctg tagttgccga aatcatcaaa gcttatgaaa
    21961 aggcggatct tcatcaagaa taagctttgc ttaggaggat aagcacatgt tgaataaaac
    22021 actgcagaaa ctacaagaaa aactcggaaa ggcagcattg cctttccttc tgttcttgtt
    22081 ttttatcatt gtttgcggcg ttacatttag tagtgtcaaa caaaaggcca atgattttag
    22141 agaaggccaa gtagcagaag agagtatccg tgcgaataaa acgatcgaga acacggaaga
    22201 aaccgaacaa agaagaaaac tagcagcaga agcagtgact ccagaatata cttatcaaaa
    22261 agatctggca gatgaccaga acaatcggat caagcaatta ttcgaattga tcgataagac
    22321 gaatgatacc atcaacaaat cttataatga caaggtagat aaggcgaagg aagatgaaaa
    22381 tatcccgaaa cctactacag aagagcggat tgctacactg aagaaaaatt ttgaaaacgt
    22441 gaatgcagaa aacgttgcgt tttaccaaaa actgccgaac aatttttatt cgacgatatt
    22501 tgaaatgact gaaacggaaa ttcacacggt tagggatgaa agtttgaagc tcattgacga
    22561 acagatgagt aaacaagttc gtgaaagcga attggaagct tttaaacaag aagcagaaga
    22621 tcagattcag tatttgaatg tcacaacaga gcagcaacaa atgatccgct acttagtgga
    22681 tcaaggcatt gtagtcaatg atgtattgaa tgagaaaaag acagaagaat taaaacaatc
    22741 tgcacgagaa gctgtacaac cagtgatgat cttccaagga gaaatcattg ttcgcgaagg
    22801 aaatcaaatt gatgcttcag cgatgaaaaa attggaatta ctaggattga cgaatcaaac
    22861 gacttcgatt ttcccactca ttgccatggt tttagctgtt ttgcttcaaa tcggtgtact
    22921 gatttataat tcacttcaat ttcaaaatgc aggagaaaga acaaaatacg tacttttcta
    22981 cgtgacagca atgtcagtca gcgtagtact aatgaaattt ttccaactct tccaaacaga
    23041 acaagctgct tatattccgc tgttttaccc cgcggctttt gctccacttg ttttaagctt
    23101 tttcttgaat cgaagagcag ggatcatggc tggtattttc caagcagtat ccgcactttt
    23161 cattttttat gattcgattg gcacgaactt tctgacagtt attttgatgt cctatgtatt
    23221 ttctggtctc atggcaacga tcgtcagacg caaacggatc tcagaacaag ggttattcgc
    23281 tgctatgtgg gtcattattt tccctgtgat gatggatgtc attttgatta tctaccaagg
    23341 aatgagcttt ggggatgcaa cgacttggtt aacgctagtc tgcggattca caggtgcatt
    23401 gttctcattc ttgctgacga taggtctgca cccctatatt gaattgatgg tcaatgatga
    23461 tagtgtgatc gtattaaatg aactcagcaa tcctaatcac cctttattga aacagttgtt
    23521 agaagaagca ccaggtacgt atcatcatag catgatggta gccaatctaa gtgctaatgc
    23581 tgtagcagaa atcggtggac gttctttact aactcgagta gcttgctatt atcatgatat
    23641 cggcaagatc aaacacgcca gctttttcgt tgagaacttg ccttcaggag cggaaaatcc
    23701 gcacaatttc ttattgccag aagacagtaa acatattatc tttggacatg tgacagatgg
    23761 tgccaaaata ttggaagatt ataatatgcc gcaaatggtg atcgatattt gcagacagca
    23821 tcatggaacc acgctgatgc gttatttcta cgttaaggca aaggaacgca acccggaagt
    23881 cacggaagag caattccgtt atcctggacc aaaacctcaa agccgtgaag caggaatcgt
    23941 caatatagct gacagttgtg aagcggcagt acgggcaatg gaccatccaa caagcgaaaa
    24001 aatcgaaagt tttgtccata atcttataga agagcgtatt tccgatggac aattggatga
    24061 cagtggattg acgttaaaag aaatccgtaa agtagaaaaa tcattgatca gcggtttaag
    24121 cagtaccttc cattctcgga tcaaatatcc taaaatgaaa tcggaagttg agaagataaa
    24181 agaagaacaa gaagaatagc aagaagaata gaaagaagcg atgtataatg gatattacct
    24241 tcatggatga aacaaaccaa gtctcagaag agaaaatgaa agaaatcgac gacttgttgc
    24301 aatttgcagc atcatattta gagttgccag aagaaacaga aatgtctgtt actttcatgg
    24361 acaacgcagc gatccaagta atcaatcgtg attaccgtgg aaaagatgca ccgacagacg
    24421 tcatcagttt tgccttggaa gaagagggtg aagacgaaat cccagtgatt tttgaagatg
    24481 gagagaatcc attgcctcgt gtactgggag acatcatgat ctctactgaa cgtgctgcag
    24541 agcaagcaga agcttacggc cattcgtatg atcgggaact tggcttttta gcggttcatg
    24601 gattcttgca tatcaacggg tatgaccata tgacaccaga ggatgagaaa gaaatgtttg
    24661 gtttacagaa agagatctta gatgcctatg gacttaaaag ataaaaacgt agagaaaaac
    24721 aaacatttcc tgatgtccgt ggaatttgcc gtccaaggag tcaaaactgt ttttgaggaa
    24781 gaacgcaaca tgaggaaaca tgttgcgttc ggaatcctcg ctttgtttat gggcgtgatt
    24841 ttccaacttg atcggataga atggttatgg ctgcttttag ctgtttttct cgtatggata
    24901 gtagagatca tcaatactgt ttttgaaaat gtcgtagaca tgtttacgga ttttcatttt
    24961 catccgattg ggaaaaaaat caaagatatg gcagctggag cagttttagt gaccgcttgt
    25021 tttgccgtca tcgttgggtt gattttattt gtccctaaaa tatatcattt gttgtttaat
    25081 taaaaaggag aacgtatatg ccagaacata aatcagggtt tgtcgcaatc gtcggccgcc
    25141 cgaatgtagg aaaatccaca ttattgaatc gaatcgtggg tcaaaaaatt gccatcatga
    25201 gtgataaggc gcagacaact agaaataaaa tccaaggagt atatactaca ccagaagctc
    25261 agttgatttt tattgacaca ccaggaatcc ataaaccaaa gcatcgttta ggagatttta
    25321 tggtagaagc agcttacagt gcgttgaaag aagtggacgc ggtcttattt atgatcagcg
    25381 ctgatcaaaa acgcggccgg ggagacgatt ttattattga acgtctaaaa aacgtgcatt
    25441 ctcctgttta tctgattatc aataaaatcg ataaaatcca tccagacgag ttgttgggaa
    25501 tcatcgaaga ttattcaacg cagatgccct ttgcacaagt gattccaatt tctgctacag
    25561 aaggaaacaa cgtcgaacgt ttgatggatg tattagtcag tgaaatgcca gagggcccac
    25621 aatattttcc agatgatcaa gtgactgatc atcctgaata cttcatcgtt tctgaattga
    25681 tccgtgagaa agtcttgttt ttaacacgag acgaagtgcc tcattctgtc gcagtcgttg
    25741 ttgattcgat gaagcgaaac gagaatgata aaatccatat ccaggccacg atcatcgtcg
    25801 aaagagatag ccaaaaagga atcattatcg gcaaaggcgg aaaaatgctg aagcaagtag
    25861 gaacgaaagc aagaaaggat atcgaaaatc ttttaggaga caaagtattt ctagaacttt
    25921 gggtcaaagt ccaaaaagac tggcgagaca aaaaagtcta tctgcaagac ttcggctacc
    25981 gaaaagatga atactgatat tctgaacttc ttataggtta agtatagaaa taaactggat
    26041 aaaaatccgc acgttgagaa aaacggcgga agatcgaaac ataaataatc tggatcggca
    26101 gaatctgctt ttccagattt attttttagg agggagaata tgagccagct tgcagaatcg
    26161 aaaggcttga ttttatctag ccgtaattac aaagaaaaag ataaattagt caaaatcttt
    26221 acggaatctt ctggcaaaat gatgttttat atcaaaggaa tccatcggca aaatcagcca
    26281 ttagctccgg ctttgctccc ctttaccgaa gcagtctata tcggtaattt tcgaagtgaa
    26341 ggtttgtcgt ttttaagcag tgtcaaagac gtccatgcgt ttcggaacat ccaagaagat
    26401 atctttttga gcggttatgg tacctatttg ctaaatctag tagatgcagc aatcgaagat
    26461 catcaatatg acccgcattt attccaattt acgcaacaag ctctgcagcg gatggatcaa
    26521 ggagatgatg cagaaatcat cacgaatatc tttgaagttc aaatactgca acgcttcgga
    26581 attgcaccaa tctggacaca ctgcgtcgta tgtggggaaa caaaaggaaa atttgactat
    26641 tcttccaaat atggtggtgt gatatgcgaa agacattggt cgatggacga tcaccgttat
    26701 catgcagatc ctcgagctgt ttatttcgtt cggatgtttt cagctatttc ttatgataaa
    26761 atcagtggaa tccatctgaa agaagaaacc aaacaagcaa tccgtgaact catcgaccag
    26821 ctgtatgatg aatatgtcgg tattcatttg aaaagcaaaa agttcatcga tcaaatgaag
    26881 tcttgggcac acgtattaaa gacgccagag aaagacatgt ctgaaaaaga tctacctaaa
    26941 aaagaagaat agtaaatgaa acatacaaca aaaaatcaaa accatttgac aaacacaggg
    27001 agttccgtct attattaacc gtagatcaaa tagatcgttc agtgagagga tctattatag
    27061 aaacaagaaa actgcaaaat gaatacgtac attgaccaag atcagtaaaa aacacgtggt
    27121 ttagcgagtt cgggagagtg ggagccggac ccaaacgatt tttgaagagg cacttgcgag
    27181 tacgataaat gaagctgccg aatctttatt cggaagtagg gtggaaccgc gaattattcg
    27241 tccctatgtc tgtaatcaga catagggatt ttttgtcgtt ttctttcctg tattcttatt
    27301 tgacagcaac gctttaaaat tgaaaggggc aaaacagatg agtaaaaaat tgaccgtcca
    27361 agatatgatt ttaacattac aaaaattttg gtcatcaaac ggctgcatgc tgatgcaagc
    27421 ttatgataca gaaaaaggag caggtacgat gagcccatat acattcttac gggcaatcgg
    27481 accggaacca tggaatgctg cttatgtaga gccatcccgc cgaccagctg acgggcgtta
    27541 tggagaaaac ccaaaccgtt tgtatcagca tcatcaattt caagtcgtaa tgaaaccttc
    27601 acctgaaaat atccaagagc tttatttaga aagcttgcgt cttttaggaa ttgatccatt
    27661 agaacacgat attcgttttg ttgaagacaa ctgggagaac ccatcgatgg ggtgtgccgg
    27721 tgtgggttgg gaagtatggc ttgatgggat ggagatcaca caattcacgt acttccagca
    27781 agtcggagga ctaccttgta aaccagttac ttctgagatc acttatggac tagaacgtct
    27841 tgcttcatat attcaagaat ttgaaagtgt ctatgatctg gaatgggctg atggtgtgaa
    27901 atatggcgag atcttcaaac aaccagaata tgaacattcc aaatattctt ttgaagtaag
    27961 cgaccaagac ttgctactaa gcaattttga ccgttttgaa aaagaagcta aacgctgtat
    28021 cgatgaaaat cttgttcatc cagcgtatga ttatattttg aaatgcagcc atacattcaa
    28081 cttattagat gctcgcggtg cagtatccgt aacagaacga gcaggttatc tttcccgtat
    28141 ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca gaacgcgaaa aattaggatt
    28201 ccctttattg aataaaaaca cagcaaatcc agaaaacatg aaggaggctg aataagatgg
    28261 caaaagactt attacttgaa atcgggctgg aagaaatgcc tgctcatgtt gtcacaccaa
    28321 gcatgaaaca attagaagaa aaaacagcaa aatttttaga tgaaaaccaa ttgacttatg
    28381 atacgatcga aacgttttct acaccgcgcc gcttagcaat caaagtaaca aacattccag
    28441 aacgtcaagc agacagtgaa gaagaagtca aaggaccagc caaaaaaatt gctttggacg
    28501 cagatggaaa ctggacgaaa gcagctgaag gatttgtccg cggacaaggt ctgacgacag
    28561 atgctatcac ttttagagaa ttaaaaggcg tggaatatgt ctatgtaacg aaatacactc
    28621 acggcaaaga agcaaaagaa gtattgacag gtttagcaga tgtcattaaa agcctcactt
    28681 tcccagtaac gatgcactgg gcagaatacg attttgaata tattcgccct attcactgga
    28741 tcgtggcatt gttagatgac gaaataatcc cgtttgagat cttggatgtc aaaacagaca
    28801 gagcttcaag aggtcatcgt ttcttaggcg aagatgtaac atttgcgcat gcaaaagaat
    28861 acgaagaaaa attaaatgaa caatttgtta ttgcttcacc gcaaaaaaga aaagaaatga
    28921 tcatcacaca agcaaacgaa ttagcagaaa aaaatcactg gatcattgat ctagatgaag
    28981 atttactaga agaagtcaac aatctagtcg aatatccaac tgtcttttct ggcgacttcg
    29041 aggaaaaata tctttctgtt cctgaagaag tactagtcac ttctatgaaa gaacatcaac
    29101 gttatttcga agtacggaac gatcaaggta tgttgcttcc tcattttatt tctgctcgta
    29161 atggcgatcg ggtaaaattg gaaaatgtgg tacgaggcaa tcaaaaagta ttgactgctc
    29221 gtttagaaga tgcagaattc ttttataatg aagacaagaa agcttcaatc gaccaatacg
    29281 ttgaaaaatt aaaaactgtt actttccatg aaaaaatcgg tagcatctac gagaaaatgc
    29341 aacgggtaca agtcatcgcc cgcattatag gcgaacaagt aggactatct gaggaagaat
    29401 tgatggatct gcaacgcgct gctgaaatct ataaatttga tctagtaacc aacatggtag
    29461 gagaattccc tgaactacaa gggatcatgg gagaaaaata tgctctgctt aaaggcgaga
    29521 aaccagccgt agcaacagcg atccgtgaac attacatgcc tatatccagt gaaggagatt
    29581 tacctgagac tacagtaggt tctgttttag cgattgctga taaattagat agtatctttt
    29641 ctttctttgc agttgggatg atcccaagtg gctcaaacga tccatatgct ttgcgccgtc
    29701 aagcatatgg tgtcgttcga atcatcgaat cgaaaaaatg ggcctttcct ttgtctgttc
    29761 tgcaagaaac gattagtgaa gttatcagta aagatacgga tcgttttggc attggcttgt
    29821 cagcgggaca acaacaggtg atcgacttta tcaaaggccg tctgcgccaa ttattgacaa
    29881 ctaagaatat tcgtcatgat gtcatcgaag ctgttttgaa tgcagaacaa aaagatctga
    29941 caaaagtatt tgcagcagca caattgttca agcagcatct tgcagatgaa gacttcaaac
    30001 catcgatgga agctttgaca cgtgtcgtga acttagcgaa aaaagcggag cttgaaaaac
    30061 aatcagaagt agaccctgaa ctgttcgaaa acgaagcgga gaaagaactg cacaaagcag
    30121 tagaggcaat gaaaacaagc tttgctgacc aaacgatcaa cgaaaattat gatcagcttg
    30181 taagccttcg cccactgatc gaaaattatt ttgatcagac gatggtcatg gcagaagaac
    30241 cagcagtacg taagaaccga ttaacgcaat taagtcaaat tgctgccatg gctttgtcac
    30301 tagcaagttt agatcaaatc atcacaaaat aatttctaat atgtaaataa accagagaac
    30361 aggcacaaaa taggctattt ctctggttta tttgttataa atataaataa ctattattaa
    30421 ttcaatgtta atttttttgt tattttattt tttgtgtaac ttttaaaaac acctatgaaa
    30481 attaaggaaa taaaaatgaa atcgattaat tgttatctta ttaatgtttt atttactttt
    30541 aaatcgtaaa ttgccctagt caagaaaaca gtttgtggta tgattatata gggaaaactt
    30601 gtttacaaat atttctgtga atatattagt ttaattatgt ttttttaaat aaaaagctgt
    30661 catttttatg taagtgaatt atatcttaat ttatctatca tcaatactta ggagtgaatc
    30721 tatggaaaag ccattcgtca agtcattgac tgctgtggcg gtaggtgcag gtgctattgc
    30781 gatctgtttg tttggatacc atatcaataa tcagcggcaa catcagcaac ggatcaatta
    30841 tgcagaatct gccatcacca atcaaaaaga caccttgact tctttatcaa aagaagtaga
    30901 caagctgtat agtacaaaag aaaaaatttt tctaaaccct gaaataacag aagaaacaat
    30961 cacaagtttg tcaaataagc tatcctctgt caaactttca gcagatgatt acgacatcaa
    31021 agaatcagaa ttgcctaaag aagctgttgc aatccaagat gagaagaaag caatcgtgac
    31081 acagttagat agtgcagaat caaaactgaa gatccagaca gctgtcaacg atttatttac
    31141 taaaaatgta tcaaactggc agcaagctgt agacgatgtc atcatcaaag aaaaggtgac
    31201 aagtgcagat gtcgctcgtg tacgtgaaaa tatgagcttt tttaaagata gtccttggaa
    31261 aacggttgtt atgcaatatc taggattcgc tgatacccag atcgcgcaag tcacgcaact
    31321 agatcagtta ttcgatacga tgttaaagga tggtcaagtg actgccaccg ctacttacga
    31381 tcaatatttg acggcgctca gtcaaataga gcagatccgg aatgaaaaaa taagcgtagc
    31441 atacgcaaca aaagcacaaa ctgttgctca gcaaatgggc tatagcaata taaactattg
    31501 acagttaaag cgatgattca agcaaggtta gcaaacggag gaaactatgg caagacgaag
    31561 aagaaaatca agagggaaaa aaatatttct gattatttta gcagttctta tcgtattagt
    31621 attagcaatt gtaggagtag gagcaaaatt atatctggat ctctcacatt ctattgagaa
    31681 aacttacgaa tcagtggaac gaacccaagg agaacgaacg aaaccagttg atctggacaa
    31741 acaagaatct ttctctgttc tattgatggg gattgatact ggagatttag gtcgggtcga
    31801 tcaaggaaga tcagatacga tgatggttgc cacagtcagt cctgaagaca aacagacaac
    31861 gatcgtcagc attcctcgag atacgtatgt cgacatcgta ggtcgtgaca caaaagacaa
    31921 gatcaaccac gcttacgcat tcggcggagc tgccatggcg atggataccg tacaaaaata
    31981 tgtagacatc ccaatcgatc actatgtctc tatcaatatg aaagggctaa aagaacttgt
    32041 tgatgcagtt ggtggtatcg aagtcaataa tgacctcact ttctcacaag atggctatga
    32101 ctttacaatc ggtaaaatca cattgaacgg ggatcaggca ttatcttatt ctcgtatgcg
    32161 ttatgaagat ccaaatggtg actatggtag acaagagcgt caaagaaaga tcgtagaagg
    32221 tgtagcgaaa aaagtattga gtctaaacgg tgtcagcaac tatcaatccg ttttgaatgc
    32281 tctagaatcg aatatgaaaa cagacatgag ctttgatgaa atgaaaaaaa tcgctttaga
    32341 ttatcgggag gcatttggta cgatcaagca agatcagctg caaggagaag gcttcatgca
    32401 agataatatc tcctatcaaa aagtcagcga tcaagaactt gatcgtgtga aaaaagaatt
    32461 aaaagatcaa atgaatctag agaataaata ggaaagtgga gaaaaaatgg aagagacaat
    32521 cagcttgaaa gaaatttttg gaacattacg caaacacttg gcaacaatca tcatctcaat
    32581 gtttgctggc ttggccatct caggaatcgt aacatttttc atcttgacac ccaaatacag
    32641 ttcacaagca caattgatcg tgacactgcc gcaatcagac acagcgaatg tcaatgatgt
    32701 caatacgaac ctgcaaatga tcaatacgta taaagatatg atcgttagtg atctagtatt
    32761 aaatgaagtc aaagatcgtc tagaaacaga agacaatgtg aaaatgaccg ctggacaaat
    32821 caaagatgcg atctccatca atcagtcaga aaactcacaa atgttctcga tccaggcaat
    32881 aagcacgaat gcagtgactg ctcaacagat cgctaataca acttcacaag ttttccaaga
    32941 gaacgcaaaa gacgtgatga atgtcgataa aatctcgatc atctcaggtg cagttgccaa
    33001 tacgacacct gtttcgccaa ataataaatt gaatctagtg attggcttag tattaggaat
    33061 gatggtaggc gtaggtttgg cattcttatt cgaattactt gatcggacag tgaaagatga
    33121 aaaatatgtg acggatacat taggattccc aatcttagga acagttccag aaatgtcagc
    33181 gaaagaattg aatgctggta tccgtaaaaa aggatcagct cctgcacgac caaaagctac
    33241 aaacgataaa acagccccat cacgcagaag ccgctcaaga gtctaggagg aagaaaaatg
    33301 gcacgaacac agaaacaaaa agcagcacaa actcatgcag tcagtttgat cacattaaca
    33361 aacccatctt caccaatcgc cgaacaatat cgtacgatcc ggacgaatat ccagtttgcc
    33421 tccgcagctg gtcagcaaat caaaacgatt gtcgtgactt catcaggtcc tggagaagga
    33481 aaatccacca ctgcagcaaa cattgcagtc gtttttgcta aatccggtca acgagttcta
    33541 ctagtcgatg cagatttacg taaacccgtc gtctataaga cattccagct gaacaatgcc
    33601 agtggattaa gtacagcact tagttcatca ggaaatgtag cagatgagat ccaacgaaca
    33661 ccagtcgaga atttgtcgat tctgccaagt ggacctaaac caccaaatcc atcagagtta
    33721 ttaagctcgc caagaatgga tcagatttta gcagaagcaa gacaactatt tgatgtagtg
    33781 atcttcgaca tgccaccagt agtagccgta acagatgcac agatcatgtc ttcaaaaacg
    33841 gatggcactt tattggtcgt acgtgaaaat acatcacgta aagaatcctt gactaaagca
    33901 aaagaactgc tagagatggt acaagcacga gtcttaggtg ttgtatacaa cggagcagaa
    33961 catagcaaag atgctggtta ttactataca gaaattaagg aataagaaga gaggagacaa
    34021 gagaaatgat cgatctacat tgccatatcc taccaggaat cgatgacggg gcagaaaact
    34081 tagaagcaag tattgcaatg gctgaaaaag cgattcagca aggcatcacg catattttat
    34141 gcacaccaca ccacaataat ggaaaataca gcaatgaaaa atcacaagtc atttctctag
    34201 tcgcttcgtt acaagccgaa ttagagaaaa gacagttgcc tttgacgtta ttggaaggac
    34261 aagaagtacg tattaccgga acgctcatcg aagacatcca ccgagatgaa attttattta
    34321 cagatttaga tgatacatat cttctgatcg aatttccgac actggaagtg ccgttatatg
    34381 cagaatggct tttttttgag ttgttagaac taggaaagac accagtgatc gtccatccag
    34441 aacgcaacgc gcattttcga gaagacccaa atcatttgct tccgttccta gatatgggat
    34501 gtttggccca aattactgcg ccaagttatg ttggtgtatt tggcaaagac atccagaaaa
    34561 cagccaaaac aatggtcaaa cataatctcg tgcagatggc tgcttctgat gcacacgggg
    34621 tctctaagcg cacattttac ttaaaagagt gctatgagca gattgccaag gactttggta
    34681 aggaaaaagt agtacagatg aaacaagtcg caaaggatct gatcaacggg gatgagatca
    34741 gttatccaat ctatgaagag gtaaaaaaga agaagtttgg tttattttag ttctttattc
    34801 gtagataaaa acaaatgagg agagagtgaa gatagtgaaa gggactgaaa gtatggaaag
    34861 tgaagattta ttatctcgta ttagtgaagt acatcctaat caagtttggt tggataaaga
    34921 aaaagttgaa aaacgatata tctatttatt ttttaaaaga atgattgata ttgtaggaag
    34981 cattattggt ttgattttag cttctccttt gatgttctat gtagcttatc gaatcagaaa
    35041 agaagagcca ggaagtccga tattttttag ccatacacgt gttggtaaaa atggaaaaac
    35101 attcaaaatg cataaattcc gttcgatgtg tttagatgct gaagaaaaat tggaagagtt
    35161 acttgatcaa aatgaaatag aaggcgcaat gttcaagatg aaagaagatc cccgtgtcac
    35221 ccaaataggt aaaattatcc gcgctaaaag cattgatgaa ttgccacagc tatggaatgt
    35281 tttaaaagga gatatgagtc taattggccc acgtccacct ttggaaaggg aagtagctga
    35341 gtacacgaaa tatgatcttc aacgcttatg tgtaaaacct ggttgttcag gtttgtggca
    35401 ggttagtgaa agaaatgatg ttagtttcca taaaatggtt gagttggatt tagagtatat
    35461 tggtcatcga agtttaatgt acgacttgag gttgattata aaaacgtttt atattattat
    35521 aaagcctaat ggagcatatt agttagggat agaatgatag attgttgata aaaaagatta
    35581 cacatgttca tattatcgtt tcaaaagtaa tagctgtaat tatgaaggtt ttgaatcact
    35641 tgtagaaaat ctaaaaaaag aaaggtgaat ggagcaattc agtatcatta ttcttatata
    35701 aaagaaaata ttctaaattt tgggatgata ccactcttaa atataatcct ctaaatgctt
    35761 taatattggt atacccgata taggaactga cgagctattt agtatgatac aaaagtatat
    35821 ttatagccat taataaattt tccaaagctt cctggaaccg tatttcctca aaacgtaatg
    35881 tatacaaatt ctattgaaaa tgtctgcaaa tcgaaagaaa ttatattgtt ttctgtacca
    35941 tcagtttttg taagaaatac agcacaaaag gtaaaaccgt atatttcaga tgaccagata
    36001 attgtagatg ttgccaaagg aatagagtca gatacattaa tgactcttac tgagattatt
    36061 gaagatgagc tgtcggagca tagaatacat ctagtagtac tttcaggccc gactcacgct
    36121 gaagaggttg caatgcaact tccaacaaca atagtttctg cgagtaagaa tcaagatatt
    36181 gctaaacgcg tatagggaat tttcacaagt ccgtatatgc gagtgtatac caataataat
    36241 gtgagaggca ttgaaatttg tggtgcatta aaaaatataa ttgcactagc cgccggtatc
    36301 tctaaaggaa tgggatatgg tgaataatgc aaaggccgca ttgataacca gaggactagc
    36361 agaactagag cgtttagtag gaagaattgg ttgttcgcct aagaccttta gtggacagtt
    36421 aattggtcag ggtgttccaa cagaactggc gataaagcaa gttggaatgg tagtagaagg
    36481 aataaatgct ctaccagcag ctgtgaaatt atccaaacag tttcaagttg agttatggca
    36541 gttgatgcaa ttatcaatgg cagaaagaag ccaaaagaat tagtagatgg gctgattaat
    36601 cgtgagcaaa aatcggaaaa taccgttcca aatatagaac agtatttttg aaacttcagt
    36661 aattaaaaga tgaggagtaa aacaatgaaa agagttatta catacggaac atttgacctt
    36721 ttgcattatg gacatattaa tatccttcgc agagcaaagg agcttggaga ttatttagta
    36781 gtcgttcttt cctcggatga gtttaactgg aacgagaagc agaagaaatg ttatttcacc
    36841 tatgaacaaa gaaagcagct tttggagtct attcgatatg ttgatctcgt aattcctgaa
    36901 acttgctggg aacagaaact ttctgatgtg catgagtatc acattgatac ctttgtaatg
    36961 ggtgatgatt gggagggtaa gtttgatttc ttaagagaag aaggcgtgga ggtggtttat
    37021 ctgcctcgta ctcctgaaat ttctacgacc cagattaaga aggatttgaa taaagtgtaa
    37081 gatataaagt ctgcagctgt taagttatag aaatattgtt aactatagga aaaatgaatt
    37141 gcggagtgtg tctagagcta tttttgtaag aggagaaata atgaatacaa agaatcaaaa
    37201 aatgaaagtg tgcctagttg gatcatcagg tggtcatcta gcacatttgt atatgttgaa
    37261 accattttgg gagaatacgg acagaatttg ggtcacattt gataaagttg atgcgaaggc
    37321 gatactgaaa gatgagcgaa tgataccttg ctatttccca actaatagaa gccttaaagc
    37381 cttgataatt aactcgataa tagcgtggag aacacttaga aaagagaaac ctgatttgat
    37441 tatttcttcg ggtgcagcag ttgccgttcc gttcttctat ttgggaaaac tatttggtgc
    37501 taaaacaatt tatattgaag tattcgacag aattgataaa ccaacagtca cgggtaagct
    37561 ggtttaccct gtaacagata gatttatagt tgaatgggaa gaaatgaaaa aggtttatcc
    37621 aaaagctata aatttaggaa gtctgttttg aaagaggaga attattatta aggaggagtt
    37681 gtaagatggt ctttgttact gtaggtactc atgaacaaca gtttaacaga ttattgcagg
    37741 ctgttgatag ttgggcggaa aatagagaag aaacggtagt tgttcaaacc gggtatagta
    37801 catacaatct cgtccattgc atatcagaaa aatttttccc ttataatgaa atgatggact
    37861 atgtccagaa ggcgagaata gtaatatgtc atggtggacc agcaagtttt attatgccac
    37921 tacagtatgg taaggttccg gttgttgttc cacgtcaagt aaaatatgaa gagcatgtag
    37981 ataatcatca actagatttt tgtaaagaag tcgaacatag aatgggtaat atcattgttg
    38041 ttgaagaaat ttcagatctt actgatattt tagataaata ttcgggaata attaatgata
    38101 aagataaaca actctctagc aataatgaac tgttttgcaa gaatttttct atagttgtcg
    38161 atgagctact aaaaaataga gggagccaaa tttgatatgt tcaaatatga cttgtcactg
    38221 attattccag tatataatgc tgaaagatat attgaagata caatatccgc agtagttaat
    38281 cagactattt ttcctaatgt agaagttata attgtgaatg atggatctag cgatgataca
    38341 gaatcaattt gtgaaaaata tgtaaaagat tatgaaaata ttaaattaat aacccaaaat
    38401 aatatggggg tttctgaggc caggaattcc ggattacgtg tagcaacggg gaattatgta
    38461 acctttcttg atgcggatga ttttgttgag gcagatttat atgaaaaaga gcttcaactt
    38521 ataaaagata attcagcaga cattggagta gtagatttca agaaagttca tgatgatgga
    38581 acaattgtta aatacagaaa aaattatgtg agaacttgga atggcgataa ttcctctatt
    38641 attaaggact ttttgtctgg gataatcggc aaccaagttg ttgataaaat tttctcgaaa
    38701 gctattatta acgggctgtc tttttctggg aaatataaaa taggagaaga catgttgttc
    38761 atgtatcaag ccttagaacg tgctgaaaaa gttgtaatgg atacatctat tgcggattac
    38821 cattatattg ttagaagcga ttctgccatg acgggaaaat ttgatgaaaa atactttgat
    38881 ccagttatta tttcagaaat tattctcgat agctgtgcat atgaaaaaga attgaaacat
    38941 tatgcacagg ctcacttgat tcacgaaata tgtaagagtt gtgagtacat ttatagacat
    39001 aaggctgaaa agatgtgccc agataagttg aagtatttgc gcaacaagct agctgaatat
    39061 agtgttttag atgcaaaaaa atatttgata aagaaacaat ttttgggttt tttattaatg
    39121 cgatttgtac ccaaaatcta tatgcttgca cataggatga tgcacatagg atgaggagga
    39181 tttgcttgca agataaaaat ttatcaatca tcgttccaat ttataatgtt gcggactatt
    39241 tggagcgttg tatgaattca ataatagcta taaaaaactt taaatatatt acagtaattt
    39301 tagttgatga tggctctact gattcatcgg gtctgatcgc agatgagtat gcaaaaagat
    39361 tcgataatgt ttgtgtttat cataaagtga atggcggatt atcagatgca agaaattatg
    39421 gtttagtacg agcagataca gaatatgtct tttttataga ttcagatgac atgttagttc
    39481 cggagacttt tgacttggtt ataagtgaac tagaacagag ttcatgtcaa gttttattat
    39541 ttgatgctaa agtagtagat gagaaagatg gttacttaga aacggtggat ggcaattatt
    39601 atgtacatgg tggtttgaga tcaaattgtt gttatacagg aaaagatgta attgaattac
    39661 agctgacaga tcataatgat tttgtaacaa cggtatggct tgggctttat aatcgagatt
    39721 ttctaataca aaacaatcta tggtttgaaa aagggttgtt acatgaggat gaattatgga
    39781 cgattaagac tctattgaat gctcgggaga tccgatattt aatggagagt gcatacctgt
    39841 atagacagag agccggatct ataatgagtt ctaataatcg taattggaat aagaatatcc
    39901 aagctgtaat atttatttat caaagtttgt atgcatatgt ggattggaag gtatctgata
    39961 ttgaatttgc aagaatgctt aagggcaatt taacacgaag atatgttcat gctttaagta
    40021 aatttgaagt atataaatac agggaaatat ataaaaaaat acagtttcgt gtgctttata
    40081 tgaactgtgt tcgaccaatt gataagatta gagttttaat tttgaaattt acgccgaata
    40141 tgtttcagtg gattactaaa ctaattggta agtcgagata gttttaacat ataacttagc
    40201 ttgacagata gaaataataa aagctcatat ctataatttc tagtcaacat cttagaattt
    40261 attatatgtt ataagtggtg agatgatacg attaggttaa tcccgtattt ttacaggaat
    40321 agtcgtgtta caacaaaatt aattttacga aagggaaatc gagaagatag ttagactaat
    40381 tcatcgatca tagaaaaatg gagattttat atgctttatt tacagctatt ttttgtacag
    40441 gaagattttg gggaatagat gttgaatggt acataagtgt aggggtaaga atacttatta
    40501 taggattatt tctggtattt cataaattta gatttaagcg caattatatt attgaaaaga
    40561 atcttaagct tgcgactgta cctataattg ctattgtagt gtattccata gcggtgtggt
    40621 tgatgtctgg aagtgttcca gatgaagatg tcgttagaaa tctttttacc gctaacacat
    40681 atttggtaat tagttatctg tttgcatctg tcctctatta tgaatttgga aaaaatagca
    40741 tagtatttct aatgaaatgt ggtgttgtat catatttact gggctcaatt ttgcccctga
    40801 taatcaagat gccgagggct gcgataccat acctatttac ttcgcacaca aatgatacat
    40861 ctttgttgtg gcttacggaa gttaacgatt taacattcgc ttttggtatt atacttttat
    40921 atttcttgtt ttcttatcat caaaaccata agaaaaagaa tattataata tgtgccttga
    40981 tgattttttg gggattaaaa cgtattgaaa ttgcggcaat tctattgtgt tatctctttt
    41041 ttaaagtcat cacggaaaaa atagtatttt cgcaaggcgc gaaaatattt ttagtgagtg
    41101 ttttatctgt ttcgtatcta tatgtaatgt ttatccataa tgggcaatta gcaatgcttg
    41161 cggagatgta caatattaac tttatgggaa gattgctaac ttataactat gttacagaaa
    41221 catattcgag cttttcacca ggttttttag gcattggatt tggccgtatc gatgaaattc
    41281 ttgatgattt agtagctgaa aactttagaa ttaattatgt ccctgttata tctttgcata
    41341 gtgatgtgct tagaatgtac ataggccttg gatttattgc gtttggtata tggatttttt
    41401 atcaatgcta ctatcgtaca atgaaattaa aaaaatattt tggctccaag actgctcaga
    41461 tatatatatg cttgactctt tatatattta ttctttattt aactgataac acttatagtt
    41521 atccgattac tggtgccaca tattttttgg ctatattatg tgtaagcgat gaaaatcact
    41581 ttaacgctta tttgaataag gagagggcta aatgacagaa aagttatcta gtgtgtgttt
    41641 gattagtgta tattttggta agtttccaga ttggataaat gtgtggtttc tgtcattaaa
    41701 acacaatcct actatagatt ttattctggt taccgatcag aatgttgata cctctgggta
    41761 tgataattta aaagtaatca atactgattt ggattctttc aagatgctat tgcagtctaa
    41821 gcttgggatg aaaaattcat catataagct atgtgattac aaattggtgt ggggattggc
    41881 tttagagaat gagatttcca agtatgacta ctggggacac agtgatatgg atgttgttta
    41941 tggtgacatc agatcttttt tgataagata taaattgtgt gactatgata agttcggtaa
    42001 tagaggacat ttgacactgt atagaaacac gaaagaggtt gctgagagat atcgtttaac
    42061 tggcagttgg ttcggatcat ataaggaaat atttaaatct aataggatct gggcatttga
    42121 tgaaacccca ggcattaatg ctatttataa cgctcacaat ttcagtcata tgaattcctt
    42181 tatatacgct gatatagatg taaattataa agatttgaga ttagtagatc gaaatgatcc
    42241 gcaatatgta aattatcaaa atcaggtatt ctattggaag aaaggaaaat tattacgtgc
    42301 ttatagtaaa gatggaacaa tggttgtaga tgaatttatg tatattcatt tacaaaaaaa
    42361 aagaatgcag aaacctgatt ttaacttaga agagtttgat ggattttata ttttaaatgg
    42421 ttactttgaa ccactagggt cctgtatcac cttagacgat gttttgaaaa ggaatattcg
    42481 aaatgatata gatttacctc aaaaaatgtt cggggggggg tacaaaaaag atttgcgatt
    42541 ttattggtta ggacgcagat atttaaatat gaagtacgac ttgaaacaag ttatcaaaaa
    42601 gttgttaaaa cgttaggata gaggagcatt atatcaggtg tacattttgg cgtttgtgga
    42661 tcatgattga gaagaaaggt tgaaacattc taatttaagc aacatcccta aaaagagcgc
    42721 ataaaaccaa gcctcctaaa agcgggctaa aaaattatgc ctaagaccta aattgtagat
    42781 agagcagtaa ctaaatatcc ctgccgttgg atgatgtaga tagcttcttc caaagaaacc
    42841 tcacggtgag ttggaggtcg gttactcttt cggtaaagtt ccggattata ggggtcgttt
    42901 ttcttgagga tgttgtagat ggtagtcaac agtattctgg caatagcgat aatcgccttt
    42961 ttgtgtcccc ggtgcttttt gagtgaaaga tagcgattcc tcaaagatat tttaacaaat
    43021 tacaacatgc tgcgttaagg ctatctgtgt ctcatttctt ttggtgcctt ggagcgcagc
    43081 ggaaaggaat gctcatatct atgaaaataa aaaagataaa agagtatata aaaaaaggaa
    43141 caatattttc gcttttaaga tataaagtat cagtgttttt gcttaaaaaa tgtacacata
    43201 ataaaaattt ggcggttttg tctagacaat gcattgttgg agaaaaactc aaaaaggaat
    43261 ttagttatgt tttagaaaat aaatacaagg atcctttcga gggaaaatcc aataaggtat
    43321 ggttttgctg gtttcaaggg tatgatgctg ctccggaatt aattaaggcg tgttatcacg
    43381 cggcaaaaca ggcgatgcca aataaagaaa taatattttt aactggtgat aatattaatt
    43441 cgtatgttca tttacctgat tatataactg aaaaatatca ttcgggcaag attggttttg
    43501 cacattattc tgatttaata cgtatatctt tattatgcga gtatggcggc ttatggattg
    43561 attctacagt gttgacaaca tcaccagaga tggcagaata cataacaagt ttgccgttct
    43621 ttgtatttaa acaattagag ttgacaagat tagattatca gccgacggta gcttcaaatt
    43681 ggctgattag ttcctattcg aataataaga ttctcttgct tacaagagat tttttatatg
    43741 aatattggaa aaaatataat tatgcgattg attattttat tttccatata tttttccgat
    43801 ttgctacaga aaaatataga gaagattggg aaaacgttcc cacatttaat aatatttcgc
    43861 ctcacatgct tcaaatggag ttgaacagtg agtataatga agaaagatgg aaacaacttc
    43921 ttctgataag cggatttcat aagcttaatc atcatatagc atacaacaga gacaatactt
    43981 tttttgaata tgtccttaat aggtataagt cttttgagga ggaatcatga aaaataatca
    44041 agacaacctt aaatatcaag taatgggagg aatgttttgg aaattatctg aaaggatatt
    44101 agcacagggc gtttcttttc tggtttcagt agtactagct agaatgttaa cgccagatga
    44161 gtacggtata gtggcaatcg tattggttct aatttcgcta gccaatgtgt ttgtgacaag
    44221 tggttttagt gttgcgttag ttcaaaataa gaattcaaaa gatatagatt tttctaccaa
    44281 tttttggtgt agtatagctg tatcagtttg tatttatttt ttgcttttta taatcgcacc
    44341 tttaatagag ttgttttatg atataaaagg gctgacctct gttattagag tgtttggatt
    44401 gataattcca ttgagtgcat ttagtgccat acaacatgca tatgttgaaa gaaatatgct
    44461 ttttaagaag tacttttttt cgacattatt tggtacagta gtttctggaa ttacaggaat
    44521 tgtaatggcc atgtatggaa tgggagtatg ggctttagtt tttcaatact ttactaatac
    44581 agcagttgat ataattgtac ttttgtttac agtaaattgg agaccaaagt tcgaattctc
    44641 tattctatcc gcaaagcata tgatgagcta tggatggaag atattggtgg ctgatttctt
    44701 tggaaccttc tttgaacagc ttcgatcact tattgttgga aaagtgtatt catctagtga
    44761 tttagctttt tataacaagg gaaatcagtt gccgacattg ataacaacta atgttagttc
    44821 ttctattatg acagttttat ttcctgccat agctaatatt agcgataatt atgaaagagt
    44881 ttatcaaata acaagacgtg cagttcaggt ggttgcatat atcatgtttc ctttgctttt
    44941 tggattggct gctgtttcgg ataatcttgt gttaatatta tttactgaga aatggcttcc
    45001 agcttctatt tttgtaaaga ttttaagtat aggttatgct ataagtatga ttggttcagt
    45061 gagtattcag tcattaaaag ctgttggcag aagtgatgta gtacttcgac ttgaatttat
    45121 taaaaaacct atatacgtgt tgctattaat attaggtgtg aatataaacg taacagctgt
    45181 tgctgttacg atggctatat ataatttgta tggagcttta gttaattcca atcaacttag
    45241 aaaagtaata ggatacaaat tcaaagaaca gattcaagat ttatttccta tgttactttt
    45301 atctacagtg atcttcgtag ttgttaatat gctttcaaat atagtcgaaa accatttatt
    45361 tacaatgata attcagattg caattggaac tggactatat attactatgt cagtattgtt
    45421 taagatagaa gtatttggtt atttgaagga aatgatagtt ttgaggttta aaagtaaatg
    45481 aagaaaatat taaaaatgat aaaacgcaaa ctacaaatgt tttgtggaaa aaccaaaata
    45541 attatagcga cagttttata tggtaagaat aatatcgaag cgcataagat tgttgttgat
    45601 aattttctgg gcaaaggata cggtggtgat tgtaaatata taattgatga attattaaat
    45661 cgaaagttat catgcgatat tgtatggctt gtaacggatg tgaatgcttc tgtaccggat
    45721 ggagtacgaa aggttcaata tggtacatta cagagtttga aggaattttt aagtgctaaa
    45781 atctggattt ctaatgttag gaatacatta aagcccacta gaagaacggg acaggtatat
    45841 ttgcagatct ggcatggtgc atatccagtg aagtatatag agggacagca ggaagatcta
    45901 cttgataagg attatgtaaa gcaagccaaa agagacggtc atgacacgac ggcgatttta
    45961 gtaaatagct tgtatatgga agagattttt aggaagtatt tctggatgaa taatcatgtt
    46021 gattatttgc gttttgggta tttgagaaat gattatcttt taaaaaacat cgataataaa
    46081 agaaaaattg aagaaactcg gaggaaatta ggaataaaaa atggagcata tgtaattctg
    46141 tattgtccaa catttagaga taatttttct agcaatggat atatacagaa tgctaaacta
    46201 tttatagaaa atttggaaag aaaatacggt agtgtcgtct tattagtcag acttcatcca
    46261 aatgacaaaa aaaatatttc attttttgaa tacaacgata aagtaataaa tggctcaacg
    46321 gtctctgatg gacttgattt agtattagct tctgacgctt tgattactga ttattcgtct
    46381 atcgcatttg atggtgctgt gttaaataaa ccagtatata ttttggagaa agatattgat
    46441 acataccgaa ctactagagg attgtcagaa cagtatgatc tttggccatt tgttaaagta
    46501 aataatgaaa tagatttggt tgaggccttt atttcatttg atagtaacaa atataaaaaa
    46561 cgtcttaatg catattatca atctgatatt tcttatgata ctggagaaac ggcacagaaa
    46621 acggtaaatt ggatgttgaa gcagatgagt ataatgtgat tagttggttg aattgaggtg
    46681 tgttagtaga tggtcaaatc agaggattca ggagtttatg tattaaaaac aattgcctgt
    46741 tttcttgtaa ttaatgtaca ttatactata gggggacagc ttattccttt ctagattaac
    46801 agtcccagta ttttttctta tttcagggtg ttatttagtg gaaaaaaaca ttacatttca
    46861 atacattaga aaaaaattgt ttctatttaa agtgcttggt atatctgtag tttttacaca
    46921 attcttaatt actatgtatg tagttttaat ggaaagatgg atgtttggtt aagccaaata
    46981 aaagagccat ataatttttt gaatcttata ttatttggta atgcttctcc atttattggt
    47041 gtgggacatc tatggtattt atttgcatta ctttacgctt atataggaat atatgcgcta
    47101 aaaagaatta ctaataaaga tagattaaag attgcaatat ttttatcaat ggtcttgtat
    47161 ctaatagaag tggtagatta ttattatggc tttactgttc ctgagtatct ttatcgcaat
    47221 gtttttttga aggatgaccc ataatcatga taggtatgaa cattcctgtc tttcagagaa
    47281 acttaagcta caaaaaactg atagctattg tgatggcagc aggaattatt tcgattgcag
    47341 agtggtgttt atttcaggaa cagttagttg tgtatataaa ttcatatata tttgcttgtg
    47401 tgcttcttat tatagctgta aaaaaatcac ctaacgtgaa caaacataat ttctttgtgt
    47461 tttttggaaa gaactgcagt ttaacggcgt tcattattca ctatgtattc attattatac
    47521 taagaggaat gaaaacaccc cggattatca agtatgcaat cgttttagta ttaagcacat
    47581 ttgtatcttt aattttttac catataaaga aattaaatgc aaatgagcag agaaatatat
    47641 gatagagatt gtatagtcac tcaagttgag tggtagtcgt cctttttgtc aaaatcctcc
    47701 acgtggcgga tgatggacaa cgttttgcat tgacgaattg ttgatttagt tttattcata
    47761 catccgcaag gagggcgaag ataaatttgt ggaatagtag gttttacagg tgcaaaacaa
    47821 gctgcgccag tattattgga tgaattatcc aagcttgaat atagaaggga tgattcggca
    47881 ggtatcgctg ttcgcgatgg agaaaacctt gtagaagtaa taaaggcacg ggtaagctcc
    47941 gcaatctgtc tgataaaaca gatgcgggta gagcgattcg cggcacttgt ggtattggac
    48001 atactcgttg ggcagcacat ggtgcgccaa gtcagacaaa tgcacatcct catgtatcgg
    48061 taattgcacc gggtctggac caggtaccgt ggaatctgag gttgttggtg tatataacgg
    48121 tattattgag aactaccagg agttgaagga aaagctccta aaaaacggat atacctttta
    48181 tagtcagact gatacagagg tattgattaa gttagtagat tattattata aaaaatataa
    48241 agtcggtcct atcgatgcca ttgcaaaaac aatggtccgt gtgcgtggca gttatgccat
    48301 ggaggtaatg tttaaggatt atccgggaga gatttacgtt gcccgaaaag attctccaat
    48361 gattattggt attgctgatg gtgaaaccta tattttctct gatgtgcccg cgattctgaa
    48421 gtataccaag caggtttact atattgacaa taatgaaatg gcccgtctgt cagcaggtga
    48481 agcagtattt tatgatttaa atggtgatgt agttccaaaa aatcttacag agattacctt
    48541 taacgcagaa gcggcggaaa aaggtggctt tgagcatttc atgatgaagg agattcatga
    48601 gcagcctaaa gccatcaaag atacaatagc atctgtggtc actgaaaatg gtatagatct
    48661 ttcgtccatg agctttactg acgaggatat taacaatatt tcacaaattt acattgtagc
    48721 ctgtagatct gcttggcatg ttggtatagc ggcacagtat gtatctgtgg atttagcaag
    48781 ggttcctgtt cgtgttgagt tagcttcgga gtttagatat tgcaagccct tgctagataa
    48841 aaatggactg gttgttgtga tctctcagtc aggtgaaata gcagacactc ttgcagcact
    48901 gcgagaagca aaggctcatg aggattaaga ctcttgctat tgttaatgtt gttggaagtt
    48961 ccattgcgcg tgaagcagtt aatgtgttct atactttggc aggaccagag atttctgtag
    49021 ccacaacaaa ggcttatagc gcgcagttga tcgcaactta tctgattgca atgcaatttg
    49081 caaaggttcg tggcgatctg aaagataatt catataatac aatgctcgat gagattcaaa
    49141 agctgccagg taaggtagag cgtgttattg aggacaaaga gcgtctttag tggtttgctt
    49201 ctaaatatcc taatgcacat aatgttttct ttattggtca tggactagac tacgctgtag
    49261 ctttagaagg aagtttaaag atgaaggaaa ttagctatat ccattctgac gcctgtgcag
    49321 caggtgagtt aaagcatggc actatttcct tgattgaaaa tggtactttg gttattggtc
    49381 tattgactca gggtgattta tatgagaaaa caatcagtaa tatgattgaa tgcaactcaa
    49441 gaggcacata tttaatggga ctcaccagtt atggtaatta cggggtggaa gatatagcag
    49501 tttttacagt atatattcct aagaccgatc cccactttgc tcctagcctt gaagtcatac
    49561 ctcttcaatt gctagggtat tatctgagca tgtccaaagg tttagatgta gataaacccc
    49621 gtaaacttgc aaagagtgtt acggtagaat aaataaagaa
//
LOCUS       DACCSK010000007        44330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCSK010000007 DACCSK010000000
VERSION     DACCSK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.007"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(143..676)
                     /locus_tag="IVP39_000360"
     CDS             complement(143..676)
                     /locus_tag="IVP39_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585464.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(973..3354)
                     /locus_tag="IVP39_000361"
     CDS             complement(973..3354)
                     /locus_tag="IVP39_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7585465.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3344..3991)
                     /gene="recU"
                     /locus_tag="IVP39_000362"
     CDS             complement(3344..3991)
                     /gene="recU"
                     /locus_tag="IVP39_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7585466.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            4055..4594
                     /locus_tag="IVP39_000363"
     CDS             4055..4594
                     /locus_tag="IVP39_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7585467.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4684..5103
                     /gene="gpsB"
                     /locus_tag="IVP39_000364"
     CDS             4684..5103
                     /gene="gpsB"
                     /locus_tag="IVP39_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7585468.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            5125..5492
                     /gene="rnpB"
                     /locus_tag="IVP39_000365"
     ncRNA           5125..5492
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVP39_000365"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5701..6867
                     /locus_tag="IVP39_000366"
     CDS             5701..6867
                     /locus_tag="IVP39_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7585469.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6913..8409
                     /locus_tag="IVP39_000367"
     CDS             6913..8409
                     /locus_tag="IVP39_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7585470.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8490..9173
                     /locus_tag="IVP39_000368"
     CDS             8490..9173
                     /locus_tag="IVP39_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7585471.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9818..11008
                     /locus_tag="IVP39_000369"
     CDS             9818..11008
                     /locus_tag="IVP39_000369"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7585472.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            11110..12063
                     /locus_tag="IVP39_000370"
     CDS             11110..12063
                     /locus_tag="IVP39_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7585473.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            12132..13481
                     /locus_tag="IVP39_000371"
     CDS             12132..13481
                     /locus_tag="IVP39_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7585474.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13664..13987)
                     /locus_tag="IVP39_000372"
     CDS             complement(13664..13987)
                     /locus_tag="IVP39_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585475.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            14116..15279
                     /locus_tag="IVP39_000373"
     CDS             14116..15279
                     /locus_tag="IVP39_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585476.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            15349..16698
                     /locus_tag="IVP39_000374"
     CDS             15349..16698
                     /locus_tag="IVP39_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7585477.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16743..17174)
                     /locus_tag="IVP39_000375"
     CDS             complement(16743..17174)
                     /locus_tag="IVP39_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7585478.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17562..20204
                     /gene="alaS"
                     /locus_tag="IVP39_000376"
     CDS             17562..20204
                     /gene="alaS"
                     /locus_tag="IVP39_000376"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7585479.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            20404..21000
                     /locus_tag="IVP39_000377"
     CDS             20404..21000
                     /locus_tag="IVP39_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7585480.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            21122..22270
                     /locus_tag="IVP39_000378"
     CDS             21122..22270
                     /locus_tag="IVP39_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7585481.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(22308..22721)
                     /locus_tag="IVP39_000379"
     CDS             complement(22308..22721)
                     /locus_tag="IVP39_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7585482.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22800..23231
                     /locus_tag="IVP39_000380"
     CDS             22800..23231
                     /locus_tag="IVP39_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7585483.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(23330..23563)
                     /locus_tag="IVP39_000381"
     CDS             complement(23330..23563)
                     /locus_tag="IVP39_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7585484.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23746..25416
                     /locus_tag="IVP39_000382"
     CDS             23746..25416
                     /locus_tag="IVP39_000382"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7585485.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25563..25949
                     /locus_tag="IVP39_000383"
     CDS             25563..25949
                     /locus_tag="IVP39_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585486.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25936..26634
                     /locus_tag="IVP39_000384"
     CDS             25936..26634
                     /locus_tag="IVP39_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7585487.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26639..27103
                     /locus_tag="IVP39_000385"
     CDS             26639..27103
                     /locus_tag="IVP39_000385"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7585488.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            27100..28011
                     /locus_tag="IVP39_000386"
     CDS             27100..28011
                     /locus_tag="IVP39_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7585489.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            28246..28785
                     /gene="pyrR"
                     /locus_tag="IVP39_000387"
     CDS             28246..28785
                     /gene="pyrR"
                     /locus_tag="IVP39_000387"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7585490.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28799..30082
                     /locus_tag="IVP39_000388"
     CDS             28799..30082
                     /locus_tag="IVP39_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7585491.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            30095..31021
                     /locus_tag="IVP39_000389"
     CDS             30095..31021
                     /locus_tag="IVP39_000389"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7585492.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            31040..32320
                     /locus_tag="IVP39_000390"
     CDS             31040..32320
                     /locus_tag="IVP39_000390"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7585493.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            32325..33401
                     /gene="carA"
                     /locus_tag="IVP39_000391"
     CDS             32325..33401
                     /gene="carA"
                     /locus_tag="IVP39_000391"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7585494.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            33406..36591
                     /gene="carB"
                     /locus_tag="IVP39_000392"
     CDS             33406..36591
                     /gene="carB"
                     /locus_tag="IVP39_000392"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7585495.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36645..37421
                     /locus_tag="IVP39_000393"
     CDS             36645..37421
                     /locus_tag="IVP39_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7585496.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            37418..38350
                     /locus_tag="IVP39_000394"
     CDS             37418..38350
                     /locus_tag="IVP39_000394"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7585497.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            38347..39063
                     /gene="pyrF"
                     /locus_tag="IVP39_000395"
     CDS             38347..39063
                     /gene="pyrF"
                     /locus_tag="IVP39_000395"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7585498.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            39070..39699
                     /locus_tag="IVP39_000396"
     CDS             39070..39699
                     /locus_tag="IVP39_000396"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7585499.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39687..40001
                     /locus_tag="IVP39_000397"
     CDS             39687..40001
                     /locus_tag="IVP39_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585500.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            40039..40680
                     /locus_tag="IVP39_000398"
     CDS             40039..40680
                     /locus_tag="IVP39_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7585501.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40711..41415
                     /locus_tag="IVP39_000399"
     CDS             40711..41415
                     /locus_tag="IVP39_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7585502.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            41412..42314
                     /locus_tag="IVP39_000400"
     CDS             41412..42314
                     /locus_tag="IVP39_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7585503.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(42472..44178)
                     /locus_tag="IVP39_000401"
     CDS             complement(42472..44178)
                     /locus_tag="IVP39_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7585504.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14260 a         7773 c         9587 g        12710 t
ORIGIN      
        1 agaaaccacg cctgtagacg tggtttcttt aattgcaaat aaatgtatga attatcttgt
       61 tattatggag aaaagctgtt tcgtttatct tttttgcatg aaaaagtaaa tgtacatact
      121 cagtagctag gatggtaact tattacaatt tattgcgata aaaataaaag ttgttatttt
      181 tattgggaaa atacccgatg tagaaaataa tgccttccaa tatacctata aaataagtcc
      241 agtattccaa aggattatat aaataagtga cgtatctatc taaaggtgta ataaaaatga
      301 taagtgataa taaaaaaata ccatttataa ctaaacataa actaatggat ttgggcggtt
      361 tcaggctcat cagaaaaata tagattctgg tgaacaacaa ccataaaaaa gtgagaatca
      421 ttgcatgaga gcttctttcc ataaattttc ctttaagagt agtgcttaaa agtacagaaa
      481 aaataataaa tgcaaggata ggagcaatta aagtaaattt atttaataat aaacaaaatt
      541 tttcagcttt tttattcttg gaataacgag gtattgtttt cgataaaaaa agatggtgca
      601 gacaaattat gataattaag catactatca gataactcaa aatgaagcct tctttcaaaa
      661 aattattcaa aaccatatat tgtatttttt atttgataat aacaatggga ttattaagaa
      721 gtggcactaa ataaaagact agtgtgtatt tagccgattt aattataaac aagtaaatgg
      781 ataaagtata tgtaatgata aaagaaaaat ggattttcta atttttaagc taaatgttag
      841 atcacacatg tatatgctgg atagtcttat aaaaaatcat gtaatacagc ttggtacatg
      901 atttttattt gtagctgttt tttacataga cacaaaaaag aaaccacgct tgtagacgtg
      961 gtttcttttt tactatctgt ttccagaagc tgaggtgctg ctggatggtg tatggtcatt
     1021 tgcagcatcc gctccgctag ttgctggctc actggaagaa gcaggaggag cagatgaaga
     1081 cggtgtcgag ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg ctggctcgct
     1141 tgtttctgct gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt gactagtaga
     1201 gctagagctt tgagtcgttg tctctgttgt tgagcttcca ggagtctgga catagctgga
     1261 agatggaata gttgttgatg gtgtaattgc attcgatcta gctgtatatt gatctttgta
     1321 gtacagttca ccgcccacac gaattaatcc gcttggcatc tcccagtctg tatttgtcac
     1381 gttagctgaa acatactgca taagctcgcg ataaacatca gaggctacgt gtgacgattc
     1441 tgaagtgaca ggtgtcattt ttttattgta acctgtccac acggaaatcg aataatttgg
     1501 cgtatagccc gcaaatagga tatctggata caccccagaa gaaataccaa gttttgcata
     1561 ctcgtcatct gtgtaattgg atgttccggt cttaccggct tgatataacc cagcgatttg
     1621 cgcattcgta ccagttcctt cagtgatcgt atcttttaaa atgtctgtga tcatataagc
     1681 tgtttctgga gacatggctg tttttccatc tggctcataa gtttcttctg ttccgtcttg
     1741 gaagacgatt ttattaacat attgaggttt gtaataagtc cctccattgg cgaaagccgc
     1801 ataggctgct gccattttta aggaagaagc tccgtatttt gtcccatctt gttcttccgt
     1861 attactagag atcgcattcg attgatggat cgtactgtat tcgatcccaa gattttttaa
     1921 gaaagaagcc actttatctg aaccgacttc attgaataat ttgactgctg gaacattacg
     1981 ggataagtaa agtgcttggc gtaaagtaat ggtgcccata tattgattat cccagtttcc
     2041 tacaggagtc gaagtccctt catagttata cggtgcgtca gtaatagttt ttccagtaga
     2101 atatttcaaa tattcaaaag ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa
     2161 atcacgggaa gtattcactg ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg
     2221 tccgccaatc tgggctttta ctttcccggt attggtgtcg ataagggtgg aggcaacttg
     2281 catctcgtcg tctgggtaag agacatactg atccgtgttg acgatatcat atagtttctt
     2341 ttgggcgtcc agatccaaat tcgtataaat ctctaaaccg tctgtgtaga cattcttatc
     2401 tgttttttct tgtacttcat tgatgacttc tttgacataa ttatcgacga tcttagtatt
     2461 gtcatcggat tgagttagtt cttgcagacc atccgttacc ggtacattaa cagcttgatc
     2521 atattctgtt tgagagattt tttcgttttg aagcatcgta tacaaaacag tatctcgtct
     2581 tttcttcgcc tgatctggat aaacataagg atcataggcg gaaggggctt gaggcatccc
     2641 agcaagcaat gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa agtacatctc
     2701 agaagctgtc tccatcccgt acaagccgtt tgacatatac actttattta cgtagtaggt
     2761 caagatctct tgtttcgatt ttttttgttc caatcttaca gccatccacg cttcttgggc
     2821 cttgcgtttc agagtctgat cctcggcact ggtggagaag aaggaaagtt tgatcaattg
     2881 ctgtgtcaaa gtacttccgc cttgcagtcc gccagaggta aagttagaga gggcagaacc
     2941 gatgatccga atagggtcaa ccccgatgtg tttataaaaa cggcgatctt ctaccgacac
     3001 gatcgcatct tctaatgttt tcggtaattc attcgcagaa attttttcac gcttttctgc
     3061 gcctaaatct tcgaatagtt cgccatcttg tgtatatagt ttggaagata cggtagcgtc
     3121 taatttttta tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc
     3181 tgctaaaaag aggatacaga aaaatgagag tattcctaaa aggatcttga taaataatcc
     3241 ttttttatgt gttccagagg actttccaga acctttgcca gaattcttct tcacactctt
     3301 tttaggagta ggctgtttct gtcgtctaga gcttctagtt tgttcatttg ccattcttgc
     3361 tttctccttc aatagataat gttgataaat agaagtcaac cgcgtccaaa taaggaatcc
     3421 gcggagaaat accagttttg atctctattc ctagttcttc gatcaaagat aaaggtagtg
     3481 atttgcgtcc tttttccttt tgggcgtccc agtaagcaat caaatgaaca ctattcaaat
     3541 aaaaacagcg cttcaaagac gaaaaccata acaagacaaa gcagacccca ttttgttcga
     3601 ggcatgcttt catatgttcg atttgatggg catggaagtt tttaaaggga aaagatgttt
     3661 tgttcttggt ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg ccattatagt
     3721 ctgtagttga ggcttgggta aagtaggctt ccttgatcac agcagcactt ctcttaggat
     3781 agtctacttt cacgatttgg ataggggtag gctttttatg gataaccgcc aacccgcggg
     3841 aaagataata tttattactt tcattgattg cttcttcaaa gcgcatcccc cgattgccga
     3901 attcagtgat ctgatttctt ttttcttgaa ttttttcgac gggagaggca ttcttataat
     3961 aaggttggcc atttggatag cgcaatacca tctttaatca cactcctagg ccacattata
     4021 gcaaaaaagc ggatagaaag aaaaggaatc tgagatggat aagtcaaaag taatctatgt
     4081 gtctgggtat cgaagtttcg aactcggtgt ttttaaagaa aatgatccta aaatccaagt
     4141 tgttaaaaat gttttaaaaa aagaactcca tcagttagca gaagaagggc tggaatgggt
     4201 acttgtcagt gggaatttgg gcgtcgaact atgggctgtg caaatagtag cggaattgaa
     4261 agaagaatat ccggaactat ctcttggtat tttgtatcct ttttctgatt ttggtaataa
     4321 ctggaatgaa gggaaccaag aaaaaaaaca attagcagaa caattagcag attatgtcga
     4381 agctgttagc catcaaccct accaatcacc tagtcagcta aagaatcaca ctcgttttat
     4441 acttgagcat acagtgggct gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa
     4501 gtattttttt gaagatgcac agaattacca agcagatcat ccctacgaaa ttcggctgat
     4561 cagtatggac gatcttcaaa attttagtga ataatcgata ttgtgtttga tttatcaact
     4621 tttttcctat acaatgaaaa aggtagaaat gttattacgt aaatgaaaac tagaggtgta
     4681 aatatggcga atttggttta tagtcctaaa gacattttac aacaagaatt caaaacaaaa
     4741 atgcgcggct atgatcccgt tgaagttgat gaattcttag acaatatcat caaagattat
     4801 gaaacttaca gcaaagaact ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa
     4861 gtagctcaat tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca aacagaggca
     4921 ccaaagagcg cagcagtgac caattttgat atcttaaaac gcttgtcaaa tcttgaacgt
     4981 gaagtatttg gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat
     5041 ccaaataact acacaaatgc agacacaagc ttagatgata atgaaaaaac acgtcaattt
     5101 taatttttga ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt gatcaggctg
     5161 aggaaagtcc atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat
     5221 aagctaaggt actctttgga gtaacggcag gaaaacaagc taaggcttcg gctatgctta
     5281 agtatccttg aaagtgccac agtgacgaag cggctgggga aacccatccg gtggaacgcg
     5341 gtaaacccct cgagcgagca acccaaacaa tggtaggggc gctttttgtc tggaagaaga
     5401 acaggacaaa aaggcagtat aactgcagat agatggttac cgctttgttt tttctccctg
     5461 gaaaagcaaa gtacagaaca tggcttatag aaaaccgcaa acatcaggga attaggtcgt
     5521 gacaaaagta atgcgttata agaaacaaga aaaacatttg aaagctattt ttcaatactt
     5581 cagatgaatc gttcttatta caaaacaaac ttttgtatca ccggttattc ggaatttgag
     5641 taaaagtgtg gggtaggact gatgtcccac ctcctttttt tataaataag gagattgtgt
     5701 atggaaaaaa acaggaagtt caacctcgtc gcaactgcgg caagcgggct agaagcactg
     5761 gttggtaaag aattaagaga tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt
     5821 tttgaaggaa caatggaaac gatcgctaca gctaatttat ggttacgtac agcagatcga
     5881 gtaaaaatcg tggttgggga attcgatgcg tatacgtttg atgaattgtt tgaaaacgta
     5941 aaagcattgc cttgggaaga ttttcttcca ctagatgcag cttttcctgt agctggaaaa
     6001 tcgatcaagt cgaaactata tagcacacct gattgccaag cgatcacaaa aaaagcaatc
     6061 gttgaacgtc tccgtaccta ttatcaccgt ccagcttctg taccactgac ggaaactggc
     6121 gcacattttc agttggaagt agctttgctg aaagaccatg tgatggtaac tctggatacg
     6181 acaggaccaa gtttattcaa acgtggttat cgcttagaaa aaggtggagc accattgaaa
     6241 gaaaacatgg cagcagcact tattatgttg acaaattggc gaaaagaccg tccattttat
     6301 gatcctgtat gtggttcagg tacgctttgt attgaagcag ccttaatagg ccataatatc
     6361 gcaccaggat tcaatcgtga gtttgcttgc gaatcttggg attggttcag tcaagaaatc
     6421 atggaaaatg ttcgatcagc agccgaagaa aaagcagatt atgatattga attagatatt
     6481 actggttcag atatcaatgg acggatgatt gaaattgcca aagccaatgc agaagaaatc
     6541 ggattaggct cttcgattac ctttaagcaa caagcagtca aagacttcaa aacggataaa
     6601 gaatacgggg tcatcgtggc taatccgcct tacggagaac gtctaggaga agaagaaact
     6661 gttcggaaat tatataaaga aatgggcgat gtattccgtc cattaaaaac atggagtaaa
     6721 tatatcttga ctagcgactt agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa
     6781 agaaaattgt acaatggtgc attgcgtacg gatctattcc agtattgggg aactcgtccg
     6841 cctagaaaaa aagagaaaga cgaataatct tactaattga ttcattcaat tggatgtttg
     6901 gaggaatagg gaatgaaaga aaaagagttt ttacaagaag taaaagaaat caatcttcta
     6961 caacaagctt taggaatcct cgattgggat acacaaacag gtatgcctga aaaagctagt
     7021 gcataccgaa gtgaagtaga cagctatctt tacagcattt atttttcaaa aaaaatcggt
     7081 ccaaaaatca aagaagccat tcgctatttt tctgaacatc cggaagaatt gtcggaagtt
     7141 ggaactgccg tttttgccaa agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa
     7201 gatcttatga gaaaatatac agctgccgtt tcttcagcac acggacaatg gcaaaaagct
     7261 agagaaacac aacaattttc tgattttcaa cacgcattga atcaaaatat tgaattaaca
     7321 aaacagttga taccttactg gagaaaagat gaaaagacga attatgatgt tttgctgaat
     7381 caatacgaac ccgggatgac tgtcgaaatc cttgataagg tatttgatca gcttaaagaa
     7441 ggaattcttt cgatccgccg tacgctccaa gaaaaaggga ctgaacctga aacagacttt
     7501 ttaaatcgga gaatgacgaa aaaacaacag aagagatttg tagtgaaagt aatcgaacaa
     7561 cttggttatg attttagtcg tggtcgttta gatgacactg tccacccatt tatgactgga
     7621 attaatccga atgatgcacg aatcacgaca cgatggaacg aaacagattt caaaatggct
     7681 gtttttggag tgatccacga ggctggtcat ggtatgtatg aacaagacat cgatgataaa
     7741 tatgcgtata caccggttta tgaaggtaca tcaatgggta tccatgaatc acaatcgttg
     7801 tttaacgaga tcattattgg aagtagtcgc agcttctgga aaaagcaata tcctttcttt
     7861 caagaatgtg cagaaggaac attcgatgat attccttttg acaaatttta tcgttcattg
     7921 aaccatacaa aatcaagttt ggttcgtatc gaggcagaca gtttgactta ccctctccat
     7981 atcatcattc gttatgaaat cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa
     8041 ctacctgaaa tatggaatca aaaatatgaa gagtatctgg gaatacgacc aacaaatgat
     8101 gtggaaggca tcctacaaga cgttcattgg tcaggtgcaa gctttggtta ttttccctct
     8161 tatgcattag ggtacatgta tgcggcacaa ttacttcatt ctttgcagca agaaatagat
     8221 gtggaaaatg ttctagcgtc agacgattat tctccaatca aaaactggat gtcagagcat
     8281 atccatcaat atggtgcatc tagaaaacca aatcaattaa tcatggatgc aacacatgaa
     8341 ccgcttaatc cgcaatacct gatcgactat atgaaagacc tgtattttaa tgtatatcag
     8401 gtaaaataaa atacaattta caaagatagc tgaaattcat ctaatctata gatgttttca
     8461 gctatttact taagaaagaa gtggaacgaa tggataaatt acaagtgatc gctgattata
     8521 gtatggacca attagcgcat gatcaaacag gacatgggag cgatcatacc aaaagagttg
     8581 tcaagttagc tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg
     8641 cagcagctta cctacatgat acgattgatg ataaagtcgt taaaaatgag aacgaggcaa
     8701 agcaacagct tcgtgtattc cttcgctctt tgccaatagt agaggaacag atctcgatga
     8761 ttttttccat tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg gccgaactat
     8821 ctctggaagg taagatcgtt caagacgctg atcgtatcga agcattagga gcaatcggca
     8881 ttttgcgtac tgcttatttc ggcggtggac atgggcaccc gatttttgat cctgaactgt
     8941 atccgcaaac attcaaggac aaagaaaatt accgaaaagg cacgacagtg atcaatcatt
     9001 tttatgaaaa actgttcttg ctgtctgata aaatgaatac tgattacgga tatgaagaag
     9061 ccaaacgaag agaaaccttc atgcgtgaat tcttagaaga attttttctg gaatggtcta
     9121 ttgacgatga aaattttact ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt
     9181 tgtgacagaa gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa gttacttctg
     9241 gaacactgtt taccaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat
     9301 ttcaaaaaat agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg
     9361 agaacaccgt ttattcggaa gaataggact gtgacaagac ttttgtcaca gtcctttttt
     9421 cgaattcaac aagtaaaact tttaagaaat attcagagag taaaattaaa aattgctgat
     9481 aaacgaagat ggaaatttta tgtattttta ttggtctatg caataaataa attaaatatt
     9541 cagaatacaa ttgattttaa cgaaagggct tctatcaatg gttgtgttgt atttatctgc
     9601 ccaggtagag taaaatagat cattatctaa aaggtaattg ttcatactat ataagtcatt
     9661 tgaaagtaaa aagatactgt aaataaaaaa tgtggttaaa taattctcat aatcagtgta
     9721 agcgttatca aaaatatagc atacttgctt tgtagatgaa tgacacgata tatgtttggt
     9781 atgttctgca agacagatga atatggaggt ggagacaatg tcttccaaag aactaacaat
     9841 gtatttagag aaagaattgg atcaactgaa agaaaagggg ttatacaata cgatcgacat
     9901 actagaaagt gaaaatggtg cttgtatcat cgtagacgga aaaaagatga tcaatctagc
     9961 atccaacaat tatcttggat ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc
    10021 aaccgaaact tatggtgtag gagcaggagc agtccgaacg atcaatggtt cgctgaaaat
    10081 ccatcagcaa ttagaagaaa aaatcgcaga attcaaaggg acagaagcag caatcgcctt
    10141 tcaatctgga ttcaactgca atatgggcgc tatctctgca gtaatgacaa aagaagatgc
    10201 tatcttatcg gatgaattaa accatgcatc tattattgac ggttgccgct tatccggagc
    10261 aaaaatcatc cgaattaaac atcaagatat gaaagacctt gagaaaaaag caaaggaagc
    10321 gatagaaagt aagaaatata aaaaaatcat gtatatcaca gatggtgttt tctccatgga
    10381 tggagacatt gcacgattgc cggaaattat tccgattgtt gaaaaatatg gactgatcac
    10441 ctacgtcgat gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca
    10501 ttttggatta agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag ctataggtgt
    10561 tgttgggggt tatgtagctg gatcgaaaac actaatcgac tggttgaaag ctagaagtcg
    10621 gccattccta ttttctactt cgctaactcc aggtgcagct gcagctgcat tggcttcgat
    10681 tactttgatg caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt
    10741 taaagaagaa ttgaaaaaag taggctataa catcggtatg tctgaaacac ctattactcc
    10801 agtcattttg ggagacgaaa aagtgactca gactttctct aagaagctga tcgaacatgg
    10861 gatttatgct aaaccaatcg tttatccaac tgtgcctctt gggacaggca ggatcagaaa
    10921 tatgccgact gcggaacata cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa
    10981 aataggaaaa gaaatggaaa ttatttaact aaatttagtt ttttcgtcta tttaaaatta
    11041 gagaacatgt tcacgcatat attgttaatc atctgaagat agtagtaaaa aagatcaggg
    11101 aggatcatca tgaaaaaagt attggtgaca ggatgtttag gacaaattgg ttcagagttg
    11161 atcacacggc tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga tatacgcatg
    11221 cctgaagtca ttgagaaaaa cgatatcttt gagatcctag atgtaatgga ttacgaaaaa
    11281 atgcgcagca tagcagaaca atatcaagtg gacacattga ttcatttggc tgctcttttg
    11341 tcagctgttg cagaagcaaa accacaatta gcctggaatt tgaacgtgac tgggctggtc
    11401 aatgcattgg aagtcgctag agaattccag ctgaaatttt ttacgccaag ttcaatcggc
    11461 gcgtttggtc caaatactcc aaaagatcat accccacaag atactcttca gcgtcctaca
    11521 accatgtatg gggtaacgaa ggtagctgga gaactactat gtgattatta ttatgtgaaa
    11581 tatggcgtag atactcgtgg tgtccgtttc cctggattga tttcttatgc aaccttgcca
    11641 ggaggcggta cgacagacta cgcagtcgat atttattatt cagcaatcaa agaaggaaag
    11701 tacactagtt tcattgatca aggtacatca atggatatga tgtatatgcc agatgcgatc
    11761 gatgccatta taaaactcat gaatgcagat cctgatcgat tggttcatcg aaatgccttc
    11821 aatatatcgg cgatgtcttt tgacccggaa gagatcaaag catctattca aaaaatcata
    11881 ccagattttg aaatggatta tgatgtggat cctgtacgtc agtcaatagc agaatcttgg
    11941 ccaaattcac tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac
    12001 ttagatgtga tgacaaagga tatgttggaa aaactgaaag aaaaattggt agagacggta
    12061 taaaacaaga accacatcca aaaagttttt tatacttttg ggattggcta tggtatgaaa
    12121 ggggacaagg gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg
    12181 aattaaaacg tacgatggga ttttttactg ctttatcgac tgttatgggc acagtgatcg
    12241 gtgcaggggt attttttaaa gccgcaagcg tagcagaagt tacaggatcc gccagtttgc
    12301 atatgttttc ttggtttctc ggtggtatga tttctgtctg tgcaggacta acaggagctg
    12361 aactggcagc tgctattcca gaaacaggag ggatgattaa atatatcgaa cggatttacg
    12421 gaaatacagc agctttttta ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg
    12481 tagcagcctt gtcgatcatt ttcggtacac agttcgtcaa tctatttggt ctttcccagt
    12541 caatgattgt tcccgtagct gttacagctg ctgtatcgat cctgttgatc aattttttag
    12601 gttctaaagc tggaggggct tttcagtcga ttacgcttgt ctgcaagctt attcctttgt
    12661 ttgtgatcgt cattttcggt ttgttccgac aagaaggagt agacttccaa ctgtttccga
    12721 tccaagcagg agaaaatctt tcatttttct cagccttggg agcgggcttg ctagcaacga
    12781 tgttcgcata cgacggttgg atacacgtag gaaatatttc gggagaattg aaaaagccgg
    12841 cgaaagattt gccaaaagca atttctttag gaatcatcgg aatcatgatc gtttatttat
    12901 tagtaaatgc ggtgttctta agaaccgcat ctatcgacgg agtcgcagga aacagcaatg
    12961 cggcaagtga cgttgcaaaa atgatttttg gcggcttcgg tggaagactg gtcacagttg
    13021 gtatcttgat ttctgtttat ggaaccatca atggctatac ccttactgga atgcgtctcc
    13081 cttatgttat ggcaaaagaa aacaacctgc cttttagcaa gctctttgct aaacttcatg
    13141 ataaaactaa agttcctgta gccgcaggta tcttagagct agtcatcgct attggcatga
    13201 tgatgatcgg tggttttgat acactaacgg atatgctgat ctttgtcatt tggatttttt
    13261 atacgatggt ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac
    13321 catacaaagt tccaatgtat ccgtttattc cactagtagc aatcattgga gggactttta
    13381 tcgtatcaag cacgcttatc actcagacct ttttagcttc gatggggata gtattgacac
    13441 ttgctggtat cccgatttat ctctatttaa aacgtagata gctaaaacat tcgaatttta
    13501 ttagaactgc acttctctgc caaacaaaat attttattta aatgaggagt acgtggaaga
    13561 aatgtcaaaa gtactcgtta attttgatca cgtaaatgtc gttttgtcta cgatgaaata
    13621 gcaaagtaaa agacagaaat cgggctcaac cgatttctgt cttttacttt gatttgcgta
    13681 actgaactat tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa ccagaaatga
    13741 aaatgggaag ccaaatcaga tagacagggt agccaagcac tttagacaaa gcatgtggag
    13801 tcattttatc catttcaaga agtgcttgct gccaagtctg aagattgaag actagcatga
    13861 aacccgaaat aatcaggaga ataaatccta aataatgaat tgtagttctt tttcttacat
    13921 aagcgatcgt agcataaagg aacagtaaaa aaataggtac gccaaaaaca aagtacatat
    13981 tcagcatctc gacaccccct ttgtgtttat cttactttac tttgaaagaa atgagaaata
    14041 atagcctcgt tacttgcttg aaacagcaaa ggactgctaa gataagagag atacaaagaa
    14101 tcaaggagga gtcatatggc aagttatatc gaaccgattc gttttgcgct gattgttttt
    14161 ccatttttag ccttagcgat ctcatctgtt ttttttattt atgaatatcg aaagtatgga
    14221 acatttctag taacaagggc agttattttg tattctttcg ttttttattt attgtgtgcg
    14281 tattttttgg tgatcttacc acttccggca aaggagacgg ttgcccaaat gacagggcca
    14341 aagatggagc tgcatttatt tgcttcatgg caacatttca gagacaacac ggtattggtc
    14401 ttgtctgaac ctagtaccta cctgccggca atgaagcaga gtgtattttt ggaaccagct
    14461 tttaatgttt tattactcat accatttggc atttatttaa gatattattt ccggctttct
    14521 tttatgaaaa cgatgattgc tagtttctgc ctttctctat tttttgaatt gacgcagcta
    14581 agtggacttt attttatcta cccaagacca taccgcttat ttgatgtgaa tgatttagtg
    14641 acaaatacat tgggtggaat cattggtttt gtcgttacgc cgttatttac atttatgctt
    14701 cctacaagaa gacgaatgga tgaactttct tatgaaaaag gaaagactgt ctcattgact
    14761 cgacgcttgg tcgcttattt gatcgactgg acatttttat ctcttattaa tttggtagcg
    14821 gcgattttct ttaaaattgc gttcgattca ggggcagtta cttctcatat ctggtggctt
    14881 ttattagaag taggggtata ctttatgatt gtaccgttta taaccaatgg atggacactt
    14941 ggcaaaaaaa ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa
    15001 gcattgatga tccgctacgg gtatctgtac tttctttttt atggtttaag ctggttgggg
    15061 cagttgcttc agcaaatcat gaattcgaca acgaatacgg tgttgcagat gagtgcagta
    15121 ttcatttctc tcggtgtctt gttactacaa ggtattttta ttttaaatat cttgctatca
    15181 tttttgagaa aaaaacggat tctgttttat gaaaaagcta gtcacacata tactgttagc
    15241 acgattggcc aaacaaagga tgatgattct cgaaattagc tgagcaaaaa cgctttgtct
    15301 gtgttataat gccaaagtcg tgataattta aagagaagag gtgcacgaat gaataatttt
    15361 gatatattcc gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag
    15421 cccacagagg tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa tatcattggg
    15481 caatctcaaa ctggtacagg aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc
    15541 aatgcagaaa aagaggaagt acaagttgtt attacagctc caagtaggga actagctgca
    15601 cagatctatg aagcagctac acaaatcgct aaattttcta caccggagat ccgtgtctcg
    15661 aattttgtcg gcggtacaga caaacagcgt caattaaatc gtctgcatca ccaacagcca
    15721 caggttgtag taggcacgcc tggtagaata ttagacatga tgaatgaaca agcgctgaag
    15781 attcacaccg catttgcatt tgttgtcgat gaagcggata tgacattgga tatgggtttt
    15841 ttaaatgaag tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt tttagttttt
    15901 tctgccacta ttccagaaaa acttcgtcca ttcttacgaa aatacatgga aaatccaatc
    15961 attgaagaaa tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg gctgatttca
    16021 acaaaaggta aagatgagaa tcaaatgatt tatcaactgc tgacaattgg tcatccttat
    16081 ttagcaatcg tatttgccaa tacaaaacag catgtggatg aaattgccga ttacttgaaa
    16141 aaccaaggct tgaaagtagc aaagattcat ggagacatca ccccaagaga gagaaaacga
    16201 gtgatgcgac aagtacagaa tctggagtat caatatgttg tagcgactga tttagctgct
    16261 agagggatcg atatcgaagg tgtctctcat gttattaatg cagaaattcc agaagatcta
    16321 gacttcttta tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg aacggccatt
    16381 acgttatatt cgccaaatga tgagcaagcc attgatgcga tagaaaaatt gggcgtctca
    16441 tttgaaccaa aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg taatagacgt
    16501 gccaaacgag aaaaatcaaa agatgtatta gatcccacgc tgattggtct tgtgaagaag
    16561 aaaaagaaaa agatcaagcc tggttataag aaaaaaatcg attgggcaat tgcagaaaaa
    16621 aataaaaaag aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg taaaaataag
    16681 aaaaattcac ataaataaaa aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt
    16741 ttttaagcta gacttttttt cattccgata caaagtactt catcttcaat aaaaggttct
    16801 gtgcaagtat gatacccttg tttttcataa aaatctacag ccgtcatttc agctactaca
    16861 taactagtct gacaaccttc tgaaaccgcc cgtttttcag cagcttcaag caactgaccg
    16921 cctaaccctt ttcctcggta ttcttttgct acgcaaaatc gttccggctt gaagcaagta
    16981 ggtgttttct tttcataacg caaagtagct actggtcttt tttgatcaag aaatagataa
    17041 taggtttgtt gcgggatatc ttttccgtcg aattctagtt ctgcaggaat cccttgttct
    17101 aatacaaaaa cttgatatct caaaaagcag ctagctgcgc gtatccattc atccgctcca
    17161 actatttttt tcatcatatt cctccttgtc atgaatagca gagatgtatt gattccttta
    17221 ttcttatcgt accaaaaatc gtggagtatg accaaaagga aatgaaaata aaaattgaga
    17281 tattgacagt gatcaataga atgcgtatac taaataagta cgaaaaggat atgatttgtt
    17341 ttttaagact cacagaaaat tctttcttgg ctgaaaaaag aatagtcact aatcaaattg
    17401 ctcccttttt ctattttggt agaaataccg acgcagctca gcgttatcga gtttaagagg
    17461 taatgattac acatcattgc aatcaaggtg gtaccgcgag ttttcgtcct tggctgtgat
    17521 ggtgtttttt tgtatttaat ttatagaaag caggtaatga catgaaacaa ttaagtagtg
    17581 cacaagttcg acaaatgttt cttgatttct tccaatctaa aggacatacg attgaaccta
    17641 gtgcgtcttt gatccccgtg aacgatccaa ccttattgtg gatcaactct ggtgttgcaa
    17701 cattgaaaaa atattttgat ggttcagttg tgccagaaaa tccaagaatc acaaatgccc
    17761 aaaaatctat tcgaacaaat gatatcgaaa atgtcggtaa aacagcacgg caccatacga
    17821 tgtttgagat gctgggtaat ttttcgattg gtgactattt caaaaatgaa gccatccact
    17881 gggcatggga atttttgact gatccaaaat ggatggcatt cgatccagaa aaactttatg
    17941 taacagttta tccaaaagat acagaagcaa aacgcatctg gcatgaagaa gttggcctgc
    18001 cactagatca cattattgat gtagaagaca acttctggga tatcggtgca ggcccatgtg
    18061 gtccggatac ggaaattttc tatgaccgtg gagaagctta taacgattta gcagaagacg
    18121 atcctgaaaa ctatccagga ggagaaaatg aacgttattt agaaatctgg aacttagtat
    18181 tttcagaatt caaccataag cctgataaca catttgaacc acttcctcat aaaaacattg
    18241 atacggggat gggattggaa cgcatggtct caatcgttca agatgcgtca acgaactttg
    18301 agacagactt gtttatgcct attatccacg ccgtagaaaa gctaagcaaa caagtaaaat
    18361 atggtgaaaa cccagtaaca gatatttctt acaaagtaat cgcagatcat attcgcgccc
    18421 tttcatttgc tattggcgac ggtgcattgc catcgaatga agggagaggg tatgtcttac
    18481 gccgtttgct tcgtcgtgct gtcatgcatg ggaaaaaatt aggtattgaa caagcattct
    18541 tatatcgttt agtccctgtt gttggggaaa tcatggtaag ttattatcct gaagtacttc
    18601 aacaaaaaga atttattgaa aaagttatcc gtacagaaga agaacgtttc aatgaaacaa
    18661 tcaatgaagg tttggaaatc ttaaatcaag tgatccaaga agtaaaaaac gaaaacggga
    18721 cgactctagc aggaaaagac atcttcaaac tatacgacac atatggcttc ccggtagaat
    18781 taacggaaga ggtggcagaa gatgaaggac tgaaagttga tcatgctggt tttgaaaaag
    18841 aaatggatgc acaacgcgaa cgtgctcgct ctgcccgcag tactgaaaaa tcaatggggg
    18901 tacaatctgc tttattgaca gatatcaaag tagaaagtaa attcattgga tacacagaac
    18961 aagaatcaga tagtcaatta ttggtcatcg ttaaaggtga gtctcttttg aatgaagtcg
    19021 cagaaggtga agctcaaatg atctttgatc aaacaccttt ctatgcagaa atgggtgggc
    19081 aagtagcgga tcaaggatgg atcatgaatg aagtaggcga aatcgtagca gttgttacag
    19141 atgttcaaaa agcgcctaat ggtcaattta tgcacaccgt agaagtaaaa gcaccaatga
    19201 gtgaaggaca aacctatcat ctattgatcg atcaatcgat gcgcaatcgg atcatcaaaa
    19261 accatacagc aacccatcta ctgcacaagg cattgaaaga agtacttggc gagcatgcaa
    19321 atcaagctgg ttctttagtc gctcctggac atttacgatt cgactttacc cactttggtc
    19381 aaatcacttc agatgaatta gctgaaatgg aaagaatcgt caacgaaaaa atctgggaag
    19441 ctatcccagt tgaaacaatt gaaacagata tcgatacagc caaaaacatg ggtgcaatgg
    19501 ctttgtttgg tgaaaaatac ggacatgacg ttcgagtagt aaatattggc ggctggtcaa
    19561 tcgaactgtg cggaggcaca catgtagcaa atacagaaaa catcggaatc ttcaaaatcg
    19621 tttctgaatc aggaattggt gcaggcgtgc gacgaatcga ggctgtaaca agtaaagaag
    19681 cgtacgaact acttgaagaa gaagaaaaac aactaaaaca agtagcaaca attgttaaat
    19741 cacctcaatt gaaagaagtt gtttcaaaag cggaacaatt gcaacagcaa gtaagagagc
    19801 tacaaaagga aaatgaacaa ttagctagca agttagcgaa tcaacaagct ggtgatgtat
    19861 tcaaagatcc agaaacgatc aatggaaaaa caatcattgc agcacaagta aaagtgaaag
    19921 atatgaatca gctgcgtcaa ttagctgacc aatggaaaca aaaaaatatt tcagatattc
    19981 ttgttttagc aaacgaacaa gaaggaaaag tcagtctgct agcggcaatg acgaaagaat
    20041 caaatgaagc tggactaaaa gctggcgact tgattaaagc aatcgcaccg aaagtaggcg
    20101 gtggcggtgg tggacgtcca gacatggcac aagctggcgg gaaaaatcct gctggtattc
    20161 cagatgcact taaagctgcg aaagaatggg ttgcgcaaca atagggcgtt tacgtttgac
    20221 agaaaaacaa aagttcaaac attgaacgaa acatgattat gggggcgaag acaaacaagt
    20281 cttgccctca tacttttttc aattggatgg cgcatctaat caaatggtcg gattcggatg
    20341 gtctttagtt gacgaaagtg acagaaattt ttacgattag tataaataaa aaggggtgaa
    20401 aaaatgaata gtagaaataa gacctttcga ctagtcttac gtgcgatctt gttagcaatc
    20461 atcatcgtac aagcgatggt tccttggctg gggtttattc cattaggatt cattagttta
    20521 acaattatcc atatcacagt aattattgct gcagtagtat taggcccaaa agatggcatg
    20581 gtgattggct tgttttgggg aatcgctaca attgttcgtg cctatgccat gccaacaacg
    20641 ccatttgata cattagtgtt cacgaatccg attatctcgg tagttcccag agtacttgtt
    20701 ggcttagtag caggcttggt tttccactgg atttatcaaa aaaataaatc aataacgatc
    20761 agctcagcct ttgcaggggt actgggttca ttggtcaata cagtcttagt gttaggattc
    20821 atgggattat tttatacagg tgcaacggca gatgcctatg gtgtagactc tgctctttta
    20881 tttaaaacct tagcaggggt tgcagcaatc aatgggatac cagaagcaat aggtgcggga
    20941 atcataacac cattactagc aaaagcgtta tttgcagcaa caccgttgaa accagaataa
    21001 tgaccatctt ttaatagtaa taggacgcac gatagataga aaaaggaaga gaggagagga
    21061 aaacaatcgt cctctccatc tttttacctg agcaacaagc aaactagaag gggtaaaaaa
    21121 cttggctaag aaacaagaag aaaaaaagaa acaaataccg caacagcgga caaaaaaagg
    21181 agtactagcg ggtatcttag gattggttac gaatctgatc ttgtttgtct ctaagctaat
    21241 gatcgggctt ttttcaggaa gtgtctccat tatggctgat gcagtgaaca gtctatcaga
    21301 tactgcttca tccatattga ctttgatcgg atttaaaatt gctgcaaagc cagcagatca
    21361 ggaacatcct tatggacacg aacgcttcga atatatcagt gggttatttg tttcgatcat
    21421 cattacttat gtaggatttc aatttttaga gtcttctgct gaaaaaatca tccatccaga
    21481 aaatgtctct ttaacaccca tcgtcttttt tgtcctaatc atttccatct ttttgaaatt
    21541 tgcacaaggc aaaatgtatc aggttattgc gcaatccatt gattctgaaa cattgagagc
    21601 tacagcaaca gatagttata atgatgtctt tactacactg gctgtattgc tttcagcagc
    21661 aatcgaatgg gtaactggat ggcgtattga tggatatata ggttttgctt tagcattata
    21721 tatcctttat agtggattga cgatgcttcg aaattttgta tacgaactat taggaagtcg
    21781 cccaacgcca gcagagattg aagaaatgga agagcattta aatgcttgcg atgatatact
    21841 tggctatcat gatttattgg tgcataacta tggacccaat aaacgctttg catcagtcca
    21901 catcgaagta gatgaatcaa tgaatttgaa tgtggctcat aagattattg atctgataga
    21961 aaaagacttt aaaaagtctt tggatgtgga acttgtctgt cacttggatc caatgcctgt
    22021 tgataacgaa aaatactata atatcttgcg gcagttaaag caaattcttc ttgaaacagg
    22081 tacagggctt aatattcatg attttagagt aattcatcaa ggaaaaacgc tgcaatttga
    22141 tgtagtggtc cctgagaaat tccagtttgt tgatacagaa ctagaaagaa tcttacgtag
    22201 tcaaatagaa gaaaaaatcg gagattacca attggaaatt acgtttgatc acaattatct
    22261 tttatattaa atgagaaact ataaaaagac tccttaaagt ttttaattta aagagtcttt
    22321 ttttctttta attgtttatg gagagcctga cgtgcaagat tatctgctcc tttatttttg
    22381 ttttcgggca accattgcag gatcaataaa gaaaattcgg atagaagttc ttggattctt
    22441 tgaaggtagg gaaggaagag ctcgttcttc gttgcgtttt tgtcgaccgt agaaactagt
    22501 gttttgctgt ctgaaaaaca gaaaatcgtt tcattctgta agtcgttttt ctttagaaat
    22561 tctaaagttt ttaaaaagac ttcgaactct gcttgatggt ttgtccctat agaaagcggg
    22621 atagatagct gttcttgttt accttgatag acaatcaaga ttccacctcc actaggacca
    22681 gggttgcctt ttgtagaggc atcaatgtat acttttaaca taaaaagaga ttctcctttt
    22741 tgttcaaact aatgttatgg taagattata tcattactga atcggtgaga ggttagagaa
    22801 tgaaaaaaaa tattcgatgg cagcctgaac tggcacatac gatcatctat tggtcattga
    22861 ccttgatcat tctgtttttt agtctgattc tttctttaga aaacacaggt ccttattgga
    22921 aaagcaattt tgttttaggt atttttcttg tttttctatt tctaggtttg cggcgttctt
    22981 ttattttaaa atcaaactct gtgattatac gttatgctgc tttttggaaa gaccgagaaa
    23041 tatctgtcgg acagattcga tctttgacgc caactaatac tggggtgcaa atgatgttgc
    23101 aagatgagaa agccccatct gttttcatga tgcgaaaaaa agatcaagta gtcctttttg
    23161 atcattttga aaaaaacgga cccaaatcag aatcaattcc ttatccttat acgaaaaaaa
    23221 gaaaaagcta attttaaaaa tcagaaccta aaaaaacgaa tttttacagg aaaagaaaag
    23281 agtgagacgt acggttcatc ccgtttagcg tctcactcta tttgtttatc tattcatgca
    23341 tactagtatc ggtagtctga acgttgcctt tcttgacatg ggcagcttgt gttcctcgat
    23401 tcccttcgat gatctcaaat tcgaccactt gattttcttc caaagttttg aatccctctt
    23461 cttcaatcgc agtaaaatgt acaaaaattt cttctgtgtc atcataactg ataaagccgt
    23521 atcctttttt gttgtcaaac cattttacaa ttcctgtggt caaagcactc acgacctttc
    23581 taaagtctga tcatagcctt cattataaag agtatttata agtacgtcaa ataaattaaa
    23641 aacctgtaat tttatgtttt ttttctgaat aaaaaaaggt ataattggca tatcttaaca
    23701 aagaagatat tttgttgaat aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg
    23761 caaatttcac aagaaacaga gctagaaccg attgttgaag tggctagtaa attagatctt
    23821 aaggaagacg atttggaact gtatgggaaa tataaggcga aaatcaattt ttctggaatc
    23881 aatcgatcaa aggataacgc tgaggggcat ttgatcttag ttacttccat caatccaaca
    23941 ccagctggcg aaggaaagtc aacgatcact gtagggttag gagacgcgct caataaaatc
    24001 aataaaaaat ctgttattgc attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa
    24061 ggtggtgctg caggcggagg atatgcacag gtattgccta tggaagatat caacttgcat
    24121 tttactggtg acatgcatgc tatcacaaca gctaataatg cattatctgc tcttttagat
    24181 aatcatattc atcaaggaaa tgaattgaac atcgatgccc gccgagtgat ttggaaacgg
    24241 gtagtcgatc tcaatgatcg tgaactgcgt aaagtaattg ttggcttggg tgggccaatc
    24301 caaggtgttc caagagaaga tgggtttgat atcacagttg ccagcgaaat catggctatc
    24361 ttgtgtcttg cggcagacct tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc
    24421 tatacatttg accgtcagcc tgtgactgct ggagacttga aagcagaagg cgctctggca
    24481 ttactgctga aagatgcaat caagccgaat cttgtacaaa caatttacgg aacaccagct
    24541 tttgtccacg ggggaccgtt tgccaatatc gctcatggct gcaatagtat tttagctacc
    24601 aagacagcat tgagattagc agattacgtt gtcacagagg caggatttgg tgcagatctt
    24661 ggcggagaga aatttttaga tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg
    24721 gtgattgtcg ccactgtccg tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt
    24781 aaaaatgaga atcttgatgc tttgaaagta ggttttgcaa atttaaagcg ccatatacgt
    24841 aatatggaac aatatcagct acctgtgatc gtcgcaatca atgaattcgt aacagataca
    24901 gacaatgaac tcacactgtt ggagcacttg tgtgaaaatc aggggattct tgccaagaga
    24961 gcgagtgtat gggcagatgg agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga
    25021 ttgattgata gaaaagaagc taattacaag ccattatacc gcctagaaga aacgatacaa
    25081 gaaaagacag aaacaatcgt caagaaaatt tacggaggaa atggtgttgt tttcagcaaa
    25141 aaagcgcaaa aacaaataga agagtttacg aagaatggat gggacaaact gccgatttgt
    25201 atggcaaaaa cacagtattc attttctgat gatccttcac tgctaggtgc accggaagat
    25261 ttcaccatca cgatccgcga aatcatccca aaattagggg caggattttt agtagccttg
    25321 actggagatg ttatgacgat gcctggtttg cctaaaaaac ctgctgcatt gaatatggac
    25381 gtgacaaatg acggagaagt gttgggattg ttttaagctt gattataatc taccatcgaa
    25441 ggagctgtat tttgactaca gcttcttttt ttagacgata gggaattaat tttgtttaca
    25501 ctaggatcat tctttttctt tattgtattt tgagctatac taaagcataa taggagggta
    25561 tgatgaaaac gacatttata agaaaaaccg gtttttacgg tatgggtagc tcgattcagc
    25621 taagaatcaa caatgaaaaa cgcagtgttc tcagtcatga acaaaagata acgcttgatc
    25681 tggaagtgcc ctttacgatg caggtcacct tttattggct aaaaagcccc gtttatacag
    25741 tgaaagaagc aaagcctgct tatgtgatca caatgaatta cttgattctt caaatctatc
    25801 cgtttttatt tctagcaact gggttggggg ccgttttcct acaaaattta tttttcagtt
    25861 tttttgcagt tattgtgttg attgcgttct ttttctatat aaaaaaccgg gcatatttga
    25921 tcaaggagga aaattatggc aaactctgat ctttttttaa tcagtttcaa tcgaatcgaa
    25981 aaatggatga gagacgagat gggaaatgca cgaaatatgg gatttaccga acttgtcaga
    26041 cgcctagctc aaagaaaaca attaatgatc agaaaatacg aagatgatct tttacagctt
    26101 gctcagttaa ggaatgcgat cgtccatgat cggatcgcag tcgattttgt cattgcagag
    26161 ccaaatgatt ggacagtcaa gcgaatccag ctgatcgaac aagaactgat tcagcctgaa
    26221 accgtgctgc ctcgatttgc aaaacgtgta acaggttttg aacaagatct tccgttactt
    26281 gaactgttaa agatagtagc agaaaaaagg tattcacaat ttccgttata caataaagga
    26341 aaatttgtag cattgattac tttacgtaat atcgggtttt ggctagcaaa agaaagccaa
    26401 aaaggtccgg ttgatttaac aaataaacga gcgttggatt tgatcatcca aaacggaaag
    26461 tacacaaatt atcattttgt tccagctaaa acacatattt acgaggttga agcgatgttt
    26521 cgtgagcaag ggactttgga agccgtactt atcactaaag atgggaatcc cgatggtaat
    26581 ctgctcggga tcgtacgacc tcgtgatatt tataaacaaa tagaaaagga ttgatttttt
    26641 gttaattatt tattggatat tcagcgcttt gctagtagga gtggatcagt tggtcaaatg
    26701 gtggatcact gataatctag cgcttggaga gacgaaaaat cttataccag gtattttgtc
    26761 actgaatcat attcgaaaca ctggagccgc atggagtatg ctagaaggga agatgtggtt
    26821 ctttactata gtgacactca ttgcagtcgt tgtgattctc acattgatga ttaaaaatcg
    26881 tgaaaaaggt aatcgctggt tcatgattgg attgagtttg attctagctg gtgcgattgg
    26941 caattttatc gaccgtattc gtcttggcta tgtagtagat atgttccaga cagattttat
    27001 gagctttccg atttttaacg tagcagatgt gacacttgta tgtggagtga tttgtatgtt
    27061 aatttatatt attttagatg aaaaggatca aagaaaaaaa tgacagaact taaaacgacg
    27121 attcaccatg aatctggtag gatcgataaa gtattgacta atttattcac cgattacagt
    27181 cgttcgcaaa ttcagctgtg gctgaaaaat ggagcggttt ctgtcaatgg acagccagtc
    27241 aaagcaaatt acaaagtaaa acaaggagac gagattactg tcttgatccc cgaaccagaa
    27301 acattagatg tggtcccaga aaatatcccg ttgacgatcg tttacgaaga tgaagatgta
    27361 gcggtgataa acaaacctca aggaatggtc gttcatcctt cagcaggtca tacgcatggc
    27421 acaatggtca atgcgctgat gtaccatatc aaggatcttt ctacgatcaa cggcgttatt
    27481 cgtccaggta ttgttcaccg aatcgacaaa gatacttccg gactgctaat ggttgccaaa
    27541 aatgaccagg cacacgaatc attagctcag caattaaaag ataagacctc tttgcgcaaa
    27601 tatgttgctt tagtccatgg agagattcct cacgaaaaag gaaccatcaa tgcgccaatc
    27661 ggccgttcga aggaaaaccg gaaaatgcag gcgataagag aagatgggaa gtcggctgtc
    27721 acacatttta ctgtattgga acgtttcgaa ggatttactc tggttgaatt aaaactagaa
    27781 accggaagaa cacaccagat ccgtgtgcat atgaagtata tcggttatcc gttagctgga
    27841 gatccagtat acggaccgaa gaaaactttg aaaggcaatg gtcagtttct ccatgcgcaa
    27901 atgttaggtt ttacgcaccc gactacaaac gaaaagatga tctttgaagc acctcttcca
    27961 gaagtttttg aaaaaacttt agaaaaatta agaaaaaaca ttgcatttta atttagataa
    28021 cggtatagta attaacaacg aaagcataaa acttaataga attcctttaa gtttagtccc
    28081 gtgaggctaa gaagggtggg agcaaggata cgtatggtgc gccataagta gattgctcat
    28141 tgacatagta tagctatcag tgtatcagta tacgctcagt ctaaacattc ctcctgtcct
    28201 cttagggcag gagttttttt atgcaaaaaa gtagggggaa tgaaaatgca agcaaaagaa
    28261 gtagtagatc aggtaaccat gaagcgagca ttgactcgaa ttacttatga gattatcgag
    28321 agaaaccata gtatccaaga tatcgtattg gtaggtatca aaacacgagg aatctacatt
    28381 gcatcaagaa ttgccgaacg cctgaagcag ctagaagata tcgatattcc tgtaggagaa
    28441 ctggatatca ctttataccg agatgacaaa aaagaaaatc cagaagaacc ggaacttcat
    28501 tcttctgaca tcccagtttc cttagaagga aaagaagtta ttttgattga tgatgttttg
    28561 tatacaggac gaacgattcg agcagcaatg gacgcagtaa tggattttgg cagaccaaga
    28621 aaaatttcat tagcagtcct ggtcgatcgt gggcatcgag aattgccaat ccgtgcagat
    28681 tatgttggga agaacattcc aacatctcgg gcggaagaga ttttagtaga gatgcaagag
    28741 cttgatggac aagatcgcat catgattttg aaagaggaag attagaggag agagaaacat
    28801 gtcggagaaa aaaggatttc ataatgatga cgtggtgtta gatatccacg atcgcccaac
    28861 cccaggacat tgggtaggat taagtttaca acacttgttc acgatgttcg gagcaaccgt
    28921 gttagtcccc atcttagtag gtatcgatcc aggtattgcg ctagtcagtt caggactagg
    28981 tacattggtg tacttattta ctactaaagg aaagatacct gcgtacttag gaagcagctt
    29041 cgctttcatt gcagcgatgc agcttttgat gaaaacagat ggttatccag cgattgcaca
    29101 aggagcagtc acaactggat tagtctattt gattgttgcg ctgatcatca aaaagatcgg
    29161 ttcagcatgg ctggataaaa tcttgcctcc aatcgtcgtc ggtccagtca tcatggttat
    29221 cgggttggga cttgcggcaa atgcagcaac aaatgctatg tataatcagg gcacgtatga
    29281 tttcaagtat attgctgtag cgctgatcac attaggatta acaattttct tcaacatgtg
    29341 gctaagaggt tttctcggat tgattccgat tttactaggt attattagcg gatacatcgt
    29401 agccttgctg tttggaatcg tggatactaa gccaatcgta gacgctgcct ggtttgctgt
    29461 tcctaacttt gagattccct ttgtacaata tgaaccaaag ctatatcttg gagcaatcac
    29521 aacaatggca cccatcgctt ttgtgacgat gacagagcat atcggccatc ttatggtatt
    29581 gaataaacta actaaacgta atttctttga agaaccgggt cttcataaga cgttgacagg
    29641 tgatggatta gcacaaatcg tggcagcctt tgtcggaggt cctcctgtaa caagttacgg
    29701 tgaaaacatt ggtgttctgg ctatcacacg tgtccatagc gtgtttgtga tcggtggagc
    29761 tgcagtattt gcagtcattt taggattcgt aggaaaatta agcgctttga ttttaagtat
    29821 ccctggacca gtcatctcag gaatcagttt tatcttattt ggagtaattg ccgcaagtgg
    29881 aatgaagatt ttaatcgaga acaaaatcga ttttgataaa aagaaaaatc tgttgattgc
    29941 ttcagttatc ttagtagtag gaattggcgg actcgtcttt gaagtaggca catttacatt
    30001 gtcggcaatg gcattagcaa ctgtacttgg catcatttta aatcttatct taccagaaac
    30061 ttcacgaagc gaagaaaact aaggaggaaa cattatgatc atcaaatcag aacgtatcag
    30121 cttaaagcat ttattgaccg ttgaggcact tactgatcaa gaagttatgg gcttgatccg
    30181 cagagggcag gaattcaagc aaggtgctaa ttggactccg caaaagcgcc agtactttgc
    30241 tacaaacctc tttttcgaaa atagtactcg cacacataaa agcttcgatg tagcggagaa
    30301 aaaactaggg ctagaagtga ttgaatttga agcaagcaca agctctgtcc aaaaagggga
    30361 aacactatac gatacagtac tgacgatgtc agcattaggc gtagacgtag cggttatccg
    30421 tcatggtgat gagaactatt atgacgaact gatacaaagt aaaacaatcc agtgttcgat
    30481 tatcaatgga ggagacggaa gcggacagca tccaacccaa tgcttgcttg atttgatgac
    30541 catttatgaa gaatttggtc attttgaagg tctgaaagtt gccatcgtag gtgatatcac
    30601 tcactctcgc gtagctaaat ccaatatgca gatgttaaaa cgtctaggcg cacaagtatt
    30661 cttctcaggt cctagagaat ggtacgacga tgaattcgaa gtgtacggac attatatgcc
    30721 attggatgaa ctactagatc aagttgatgt catgatgatg cttcgggtgc aacacgaacg
    30781 ccatgatgga aaagaaagtt tctcaaaaga aggctaccat caggaacatg ggttaacagt
    30841 tgagcgtgcg aagaagttgc aaaaacatgc aatcattatg cacccagcac cagttaaccg
    30901 tgatgtagaa ttagctgatt cattagtgga aggattacag tcgcggatcg ttcagcaaat
    30961 gagcaatgga gtattcgtca gaatggcaat cttagaagca gtgctaaatg gaaaagcgta
    31021 attataggag gatgaaaaaa tgaaaacact cattaaaaac ggtcagatca atacacgaaa
    31081 aaatatgacg acaccagcag aaatttggat agaagatggg cgaatcaaag caatcggtac
    31141 aggattctca gaagccgaat ttgatgaagt gtttgatgca aagggacaat tgatcacacc
    31201 tggtttagtg gacgtccatg ttcatttgag agagccagga tttacataca aggagacgat
    31261 cgaagctggg actcgctcag ctgctcgtgg cggtttcact actgtatgcg cgatgccgaa
    31321 tttaaatcct gtacctgata cggcagaaaa gctgagacaa gtatatgaca tcattagaaa
    31381 agatgcagtt gtgaaggtat tgcagtatgc gccaatcaca gaaaatctgc gaagtgagaa
    31441 attagttgat caagaagcac tgattgaaga aggtgctttt gctttcacaa atgacggtgt
    31501 aggtgtccaa acagctggga cgatgtatct ggctatgaaa gaagctgcaa aaaacaataa
    31561 agccctagta gcccatacag aagatgaatc tttattattt ggcggcgtta tgcacgctgg
    31621 caagaaggca gaagaacttg gtcttcccgg gatcttaagc gtaaccgagt cttctcaaat
    31681 tgctagagat ctgttgctag ctgaagcaac tggtgttcat taccatgttt gccatgtttc
    31741 tactaaagaa agtgtccgtg taatcagaga cgcaaaaaaa gcaggaatcc acgtaacagc
    31801 agaagtttca ccccatcatc ttattttgat cgacgaagat attcccgaag atttcggctt
    31861 ttggaaaatg aacccgccgt taagaggacg ggaggacaga gaagctttga tcgaaggctt
    31921 gcttgacggt acgatcgatt gcatcgctac agaccatgcc ccccatggat tagaagaaaa
    31981 aagccaaagt ttcatgaaat caccatttgg tatcgtagga agcgaaacag cgtttcaact
    32041 gatctatact cattttgtag aaactggtcg attcacgttg gaacaagtga tcaattggtt
    32101 ggcagttaaa ccggctgaga tttttggttt gaatgcagga acgctaacgg ttggtgcccc
    32161 cgctgatgta gcagtttttg atattacaca aacttgcacg atcgataaag aagacttttt
    32221 atcaaaaggg gagaatacac cttttatcgg ctggaaagta aaaggcgaaa cacaaatgac
    32281 ttttgtcaac ggaaaacttg tgtggcagaa gggagaataa aagtatggaa cgtttgctta
    32341 tattagaaga tggaacagtt ttcgaaggaa aggcttttgg tgcagatgct agtgtcttcg
    32401 gagaaatcgt ttttacaaca agtatgactg gttatcaaga aactatcact gatcaaagtt
    32461 ttaacggtca aatcattaca tttacctatc ctatggtggg taattatggg gtaaatagag
    32521 atgattatga atcgatttct ccaacatgca aaggagtagt agtaaaagaa catgcacgtg
    32581 tagcttcaaa ctggcgaaac caaatgacgc tagattcatt tttgaaacaa aaaggaattc
    32641 ctggaatatc aggtatcgat acgcgcgccc ttactcgcaa aatccgtgaa gtcggaacga
    32701 tgaaagctag tatagttgat gctggagatt ccttcgaaca tgcatttgat cagctgaaag
    32761 caaccgttct agcaaccaat caagtgcagc aagtctcaac taacaaacct tatccaagtc
    32821 caggaacagg tcggaaagtc attgtcattg acttcggctt gaaacatagt attttacgag
    32881 aattgagtaa aagaaactgt aacctcacag tacttcccta taacacagat gccgagacga
    32941 ttttagcgct atcaccagac ggagtcatgc tgacgaatgg acctggtgat ccaaaagatg
    33001 tgccagaagc aatcgaaatg atcaaaaaaa tccaaggaaa agtaccgatt tttggtattt
    33061 gtctcggtca ccagttattt gctttagcca acggtgcaga tacctataaa atgaaattcg
    33121 gacatcgagg attaaatcac ccggtacgag aagtagcgac cggaagaatt gattttactt
    33181 ctcaaaatca tggctatgca gtcgatgaag ccacgattga tccagaaaaa ctcatcgtaa
    33241 ctcatgtaga agtaaatgac ggaacagtag aaggagtcaa acacagagat tatcccgctt
    33301 tcagtgtgca gtttcatcca gatgctgccc ctggtccgca tgatgcacta catttatttg
    33361 atgaatttat ggaaatgatg gacgcaggaa aggagcagta aacagatgcc gaaacgtaca
    33421 gatatcaaga aaattatggt gattggatct ggtccgatca tcattggtca agccgcagaa
    33481 tttgactacg caggaacaca agcctgcctt gccttaaaag aagaaggata tgaagtggtt
    33541 ttagtaaatt ctaatcctgc gacgatcatg acggataaag aagtggcaga tcaagtatat
    33601 atcgaaccga tcacacttga atttgtatca aggattcttc gaaaagagcg tccagatgct
    33661 ctccttccaa ctttaggcgg acagacaggc ctcaatatgg caatggagct ggcagcatca
    33721 ggtattttag aagaattgaa catagaactt ctaggaacaa agctttctgc gatcgatcaa
    33781 gcagaagatc gtgatctatt caaacagttg atgaaagaat tgaatcagcc aatccccgag
    33841 tctgaaatcg tcaatacagt agaagaagct gtagattttg caaataaaat cggctatcct
    33901 gtcatcgttc gtccagcctt tactttaggc ggaactggtg gaggtatgtg tgaaaatgaa
    33961 gaagaattac gtatcgtatc agaaaatgga ttaagtcttt cccccgctac ccaatgtttg
    34021 attgaacgaa gtattgcagg ttttaaagaa attgaatatg aagtgatgag agattcggct
    34081 gataatgcaa tcgttgtctg caatatggaa aattttgacc cggtgggtat ccatacaggt
    34141 gattctatcg tatttgcccc tagtcaaaca ttgtctgacc atgaatatca aatgcttcgt
    34201 gatgcttctc tttcgattat tcgagcactt aagatcgagg gaggctgtaa tgtccaactc
    34261 gctcttgatc cacatagttt taattattat gtgattgaag tcaatccgcg tgtttctcgt
    34321 tcatctgcgt tagcaagtaa ggcgactggc tatccgatcg ctaagctagc tgctaaaata
    34381 gcgcttggat taacattaga cgaaatgaaa aatcctgtta caggaacgac ttttgcagaa
    34441 tttgaacctg cattagatta tgttgtagct aaaattcctc gctggccgtt tgataagttt
    34501 gaaaacggag aacggctttt aggcacgcag atgaaagcaa ctggtgaagt gatggctatc
    34561 ggtcggaata tcgaagagtc tttattgaaa gctgtgcgtt ctttagaaat cggtacacac
    34621 catatcgaac ttcctgagtt ggcagacatc acggacgatg acctaatcca aaaagtggtt
    34681 cgagcacaag atgatcgcct gttttatgta gctgaagcta ttagaagagg gtacaccata
    34741 gaagaattag cggatttgac aaaaatcgat ttattctttt tagataaact gttgcatatc
    34801 gtagaaatcg aacagcagtt gaaacagcag ccgttggatg acaagatatt gaaaacagcg
    34861 aaacaaaacg gttttacaga caaaaaagtg gcggagctgt gggaaataaa cgaagaagcc
    34921 gttcgccaga tgagaaaaga aaatgatttg attcctgtat ataagatggt tgacacttgt
    34981 gcagcggaat tcgaatcaaa aacaccttat ttttatagtt cctatgaata tgaaaatgaa
    35041 agttcacgat cagaaaagcc ctcaattcta gtactgggat ctggtcctat ccgtatcgga
    35101 caaggggttg aatttgacta tgcgactgtg cattctgtca aagcaatcca gcaggcagga
    35161 tacgaagcaa tcatcatgaa cagcaatcct gaaacagttt cgactgactt ttctatttcg
    35221 gataaattat attttgagcc attgaccttc gaagatgtga tgaatgtgat cgagctagaa
    35281 cagccgatag gagtaatcgt tcaatttggt ggtcagacag caatcaactt agccgaacct
    35341 ttgactgctg ctggtgtaaa aatcttaggg acaacgatcg aagacttgga tcgtgcggaa
    35401 aatcgtgatt tattcgagca agcgctgaaa gagttggata ttcctcaacc tttaggggat
    35461 actgctacaa gtaaagaaga agccgtggtt atcgcttcga gaattggtta tccagttctt
    35521 gttcgtccaa gctatgtatt aggcggacgt gcgatggaaa tcgttgaaaa ccagcgagat
    35581 ttagaagatt acatggaaca tgctgtgaaa gcatctccag aacatccagt actagtggac
    35641 cgttacttgc ttggtggtga atgcgaagtg gatgcgattt gtgacggtga aacagtactc
    35701 atcccaggga tcatggagca tattgagcgt gctggtgtcc attcagggga ttcaatggct
    35761 gtgtatccac ctcaaacatt atctgaatcg atcaagaaaa cgatcgaaga ttacacgatc
    35821 cgcttagcat taggtttgaa ttgtgtcggg atgatgaata tccagtttgt catccatgaa
    35881 aatgaggttt atgtgatcga agtcaatcct cgagcaagtc ggactgttcc attcttaagt
    35941 aaggtgacag gaattccaat ggcacaaatt gcgacgaaag ctattttggg agagaaacta
    36001 aagacacttg gttatgaaaa cggactttat ccagaaacaa aagaagttca tgttaaagca
    36061 cctgtctttt cttttacgaa attgcagaaa gttgatactt acttagggcc tgaaatgaaa
    36121 tcgactggag aagtaatggg atctgacaga caattagaca aagctttgta caaggcattc
    36181 gaagcttctg gattacactt gcctgaatat ggagccgtat tatttacgat tgcggacgaa
    36241 acaaaagaag aagcactaga attagctaaa cgttttacag aaattggcta tagtctgata
    36301 ggaacgaaac atacagctgc ttatttcgaa aaaaatggtc tggtcgttga aacagtagcg
    36361 aaaatttctg aggaagcagc agaaaaaaat gtagtcgact taataagaga tggtaaaaca
    36421 caagtggttg tcaatacgat tgataaagat cggggaaatg cttctaaaga cggtttcatc
    36481 atccgaagag aagcagttga acatggtatt ccattgttta cttctttaga cacagcagat
    36541 gcgatcgttc gagtgatgga atcccgtgct ttttcaacta accctattta acggcgagca
    36601 catgttaaga caaaaagacg aaaaaccgtt gcaggagggg acagatgaaa caagaaatga
    36661 tggaaatcgt tcagcaaaaa cagctggcac caaagatttt tgaaatgaca ttgaaaggtg
    36721 atctagtaaa agaaatgcag caaccaggac agttcttgca tattctcgtg ccgcgtgcag
    36781 acttgctatt acgccgaccg attagcttga atcatatcga ccacgaaaca aatacttgcc
    36841 gtatcattta tcgggtggaa ggtgagggga ctagagtctt ctctactctt tctgccggag
    36901 accatttaga tgtcatgggt cctttaggca atggttttga tttgtccaat cttcatacag
    36961 gagacgaagt atttataatc ggtggaggaa tcggtgtccc tcctctatat gagttatcaa
    37021 aacagttagt cgaaaaagga attaagccaa cgcatttttt aggttttgcc acacatgaag
    37081 tcgtttatta tgaaaaagaa tttaatcgtc taggagaaac aagaatcgct actgacgatg
    37141 gaacatacgg tatgcacgga aatgtcggta atttattgtt aggaacgaag acggaaccta
    37201 cagctgtttt tgcatgtggt tcaaacgggt tattgaagac agtggaacag ctgttttctt
    37261 cgcatcaaaa tgtccagtta tcattagaat cccgcatggc atgtgggata ggggcttgct
    37321 atgcttgcgt ttgtcatacg ccggaagatg aaacaggaac aaaaagtgtg aaagtttgtg
    37381 acgaaggacc gatttttaaa ataggagagg taattatatg aatgagaatc ctttagcggt
    37441 caaacttcct ggcctagaat taaaaaatcc tatcatgccg gcaagcggct gtttcggttt
    37501 tggcaaagaa tacggaaaat actatgattt gaatcagctt ggatcgatca tggtcaaggc
    37561 aacgactcca aatgcccgct ttggcaatcc cacgccaaga gtggcggaaa caccatctgg
    37621 catgttgaat gcaattggtt tgcaaaatcc tggtctagac gttgttatgc ataaacttct
    37681 tccagaatta gaggagtaca atgagttacc catcatcgca aatgttgctg gtgcttgtga
    37741 agaagactat gtagaagttt gtagcaaaat tggcgaagca ccaaatgtga aagcaatcga
    37801 gctgaacatt tcttgcccaa atgtcaaaca tggcggaatt gcttttggaa cagattctga
    37861 tgttgctttt caattaacac aagcagtaaa gaaagtttct tctgttcctg tctatgtcaa
    37921 attgtcccca aatgtaacag acatcgttcc aatcgctcag gcaatcgaag ctggaggggc
    37981 agatgggttt acgatgatca atactcttct tggtatgcgg attgacctta agacaagaaa
    38041 accgatttta gccaatcaaa caggtggact ctccggacca gcaatcaagc ctgtcgctat
    38101 tcgtttgatt caccaagtag caagtatatc cagccttcca atcattggga tgggcggcgt
    38161 gcagacagtg gatgatgttc ttgagatgtt tatggctgga gcaagtgctg tagctatagg
    38221 gaccgcgaat ttcaccgatc cgtatatctg tcctaaacta attaaagagc tccctgtgcg
    38281 aatgagcgaa ctaggcatcg aatcgctgga aagattaatc aaagaagtga gagaggaacg
    38341 tgaacgatga atcaactaga ccaacgacca attattgcat tagatttttc aactaggcaa
    38401 gaggtagaag actttcttcg atttttcccg aaagaagaaa aattgtttgt gaaaatcgga
    38461 atggaattgt tctaccaaga aggacctgaa atcgtacgtt atctaaaaga ggctggtcat
    38521 aatgtatttt tagatctgaa acttcacgat attccgaata cggtcgaaaa agcaatgcgg
    38581 ggattggcaa agcttggtgt tgatctcacc tgtgttcatg ccgctggcgg tattcgtatg
    38641 atggaggcgg caatgcgagg attagaagag gggactccag aaggtggaaa acgtccgcta
    38701 cttctagcta tcactcagct tacttctaca agtgaagaag aaatgcatgc cgatcaacta
    38761 atcgaagttt ctttagaaaa aagtgtgatc cattatgcta gctgtgccaa aaaagcagga
    38821 ttagatggtg tggtttcttc tgcttgggaa gtagaagcga tcaaagaaat ggcaggagac
    38881 gaatttgttt gcttaactcc gggtataaga ccagaaggaa cagtcgcagg agatcagaca
    38941 agagtagtca caccttctca ggcaaaaaag atcggctcaa cttttatcgt tataggtcga
    39001 ccaatcactc agtcaactaa tccttataaa gcatatcaaa cgattcaaac agaatggagc
    39061 taacctaaaa tgaatgttga acaaagtatc gcaaaagact tattagaaat tgaagcagta
    39121 tttttaaatc caagtaatcc ttttacatgg gcaagtggaa tcaaaagccc aatttactgt
    39181 gataaccgaa tcacgatgag ctatccaaaa gtccgaaaag agatcgcaaa aggattagca
    39241 agcaaaatca aagaagctta ccctgaagta caagtgattg ctggaactgc aacagcagga
    39301 atccctcatg ccgcatgggt agcggaaata ctggatttgc caatggtcta tattcgaagt
    39361 aaggcgaaag atcatgggaa aggtaaccaa atcgaaggac gtatatttga aggtcagaag
    39421 atggttgtga tcgaggattt gatttctaca ggcggaagtg tcttggaagc agctgaagca
    39481 gcaaaaagag aaggtgcgga tatccttggt gttgcggcta tctttacgta tgagttgcca
    39541 aaaggaaaag caaatttcga aaaagccgaa atccctctta tgacgttgac caattattct
    39601 gtattgattg aagcagcttt agaagatcgt tatatcgatg agcaagaatt aactctgcta
    39661 aaagaatgga aaaaagatcc tgaaaattgg cagatttaag cgcgcttgat gaagatagag
    39721 aaatccgatt tgtgtctcca gaatcatcaa ttggaatagc aaaaatccaa aaactgattg
    39781 cgagaagcat cttttcatca ggtatgaaaa gaaacttcta tcgtgatcag tttttgattt
    39841 ttttaaaatt catttttcta agactggtta gcagaaagaa gagaataatc tcttctcttt
    39901 tttctattat atatagattt tctctatttt ttggtaaggg agctgtttca aaaatggtaa
    39961 agagcggaaa tgttgcgaat atcagttatt taatgatatg atattcggaa aaagaaatct
    40021 caaagggagg taacttttat ggggttgaat gaatttattt taggattgaa caatttagag
    40081 acgatcacaa gggcacctgg attttttaaa tatacagagc atacagttgc agcacattcg
    40141 tatcgtgtag catctattgc gcaagttcta ggagacattg aagaagcgaa tggacaaccg
    40201 atcgattgga aactgctcta tgaaaaatca ttgaatcatg actacacaga gcgttttatt
    40261 ggtgatatca aaacgccggt gaaatacgca agcaaagagt taagagggat gctgcaaaca
    40321 gttgaagaaa agatgacgga cgaattcatc aaccaagaga ttccagctga gttccaagaa
    40381 gtttatcgcc gtcggttgtt cgaaggaaaa gatgaaaccg tagaaggaga actattagcc
    40441 atttcagata aggttgacct tctttatgaa tcttttgatg aaatcatcaa aaataacccg
    40501 gaaaaagttt ataaggagat gttcattgag gcagtgacta cgattaaaag ctttgaacat
    40561 cggccttctg ttcagtattt ctttaaagag atatttccgg aactattgaa ccaggaattt
    40621 tatggaaaag aggcgttttt agaaaccgtc tcaacgattg taaagaaaga agaaaaataa
    40681 aaatgatatg agatgcaggg ggaagtatag ttgaaaaaga atatggatgt cgaatggggc
    40741 tattctggag acaaaggtcc ggaaaattgg gcgagtttat gtgattggtt tgcgagaggt
    40801 gcggaatttc cattgcagtc gcctattcat ttgaccaagg gggaagaaac caaaatagaa
    40861 ggagacacgc tttcctttca ttatcaaaag caacggttta ctgacaagga attcaaaaac
    40921 acgattcatt tagtaccttt tgaccaactc agttatgtag agttcaagaa agaacgctat
    40981 tatttgacag atattcattt ccacttgcct tctgaacata tcatcaacgg aaaacaggaa
    41041 gatattgaat ttcattttgt tcatatgaac agcaaaaaag agaatctagt tgtgggtgtc
    41101 atgtaccagc taatggtaca ggaaggttgg ctgtataatc aagaaaatgg ggaaagttgg
    41161 aacttagaaa aacatgagca ttgggtaaat ccggatgtgt tctttccgga gcaaaaaagc
    41221 catttccatt atattggctc attgacaact ccgcctacca gcgggccaat ccaatggttt
    41281 gtaatggatc atgtaggaaa actgaaccaa gagtttcttc tccccttcga tggtcagtat
    41341 gctcgaccga acaaccgacc aatccagccc ttgaatggaa gagaaattta ttatttcgag
    41401 gagtttgagc aatgaatatt cagcagatga aatacgtggt cgctgtagca aacaatggta
    41461 gtttcagaga agcggcaaag aaaatgtttg tctcccagcc aagtctttca gctggtatca
    41521 aagaattaga aaccgagtta gggatcacgc tgtttactcg aacgaatcga ggggcatatt
    41581 taacagaaga agggcaagac ttcttaaatc gtgccgagaa aatattggtc cagcttgata
    41641 cattagaaac tcactatcta acgagtgaaa agagagacaa tttttcgatc gctgcccagc
    41701 attatgattt cttaggtcct ttgaccgcac agctactcga ggaattcaaa gacgagatca
    41761 aacaatttcg gatcatggag acgacaacag cgagagtgat cgaagaagtc aaagagcagc
    41821 acagtgagat cggcattatt tatatgaatg agcataatca agctggtatc agaagatatt
    41881 tacaacaagg agagctaagt ggccgcagca ttggaaaatt caaaacgcat atttttttaa
    41941 ggcaaggaca tccattagct gaacacgcag agatcaccaa agaagaattg ttatcttatc
    42001 ctcaagttcg ttttacacag gatggaaata atttcccgta tttttatgaa gaccttatcg
    42061 agatccctga tcaagaaaca gtgatttata cgagtgatcg aggtactttg atgaatatcg
    42121 ttcttgaaac tgatgcttac gcttcaggat ctggtattgt gattggaggg attcgcgagc
    42181 acttgcggtt aatccctttg gcagaaggcg aactaaatga attttatatc atttattcgg
    42241 cgaaacgtcc gctcagtgaa atcgctcaaa gatttatcaa agagcttact cggttactgg
    42301 accagcagag ctaagaaaag cagatgagaa cgctgtttat tgaacaccgt tgatcaaaaa
    42361 agaggggttg tgacagaatt ttgtcacaac tcctcttttt gtagcaaaca atttataact
    42421 aattgctgtt gtgggaaagt acgcaaatat gattctagaa acactcattg attacctgtt
    42481 ttgtttcata gctgtaattt tttcttcttc tggtgtcacg ataatcgtct tttggttttc
    42541 ataaataaca taaccaggct tggcgccatt tggttttcgg atatgcttta cctgtacaag
    42601 atcaaccggc acttgggcag agtatcggta ttttgaaaaa taggcagcta actcggcagc
    42661 ttctgtaatt gtttcatctg agggcttgtc acttttaatg atgacgtgcg aaccgggtat
    42721 attttttgca tgaagccaat agtcggtttt tttagctgtc ttcattgtca gctggtcatt
    42781 ttgaagattg tttttaccaa caagaatgag cgttccgtca gaagaaaagt attgatcagg
    42841 ctgagatggt ttcttacgtt tttgtttttt taagttcttc tttttcaggt atccttctga
    42901 agtcaattct tctttaattg cttcgatatc cattgggccg gcaatttcca gttgagataa
    42961 gacagattca agatattgga tttcatcttt ggcttcttgg atttgttcgc caatcagctt
    43021 aacagcgttc ttcagctttt ggtatcgatg gaaatatttc tgtgcatttt gatttggtgt
    43081 caatgccgga tctagtgcta ttttgattgg ccgatcttct tcataataat taggtaaaac
    43141 aacttctttt gcgccgcgtg gtacttgtgt cagaaaagta gtaagtagtt cgccatttct
    43201 acggtaattc tcagcgtttt cggattcttt gagtgtttgt tcacgttttt tcaatttatt
    43261 tttatttcgt tttagttcat tttcgatttt tcgaatcagt tctccgcctt gttgttttgc
    43321 ccgatctttc tctgcttttt cgtgataata gctatctaat aattgactga gtgttggata
    43381 agctgttaca gcagatgcct gttcggacaa tacctgataa gcaatcggtg tgaaaaaatc
    43441 tttattttcc gtttcgtaaa aagtaggaat aggctgatgg ctgatggctg agaaaaattc
    43501 gtcccataca accatctttt cattaggacg ttccagtaat cttttggtca gctcttgagc
    43561 gatatcgaaa ccgattccct gaaactgacg ttggattgct tttggatcta gttcagtttg
    43621 ggaaagccgc tgaaagattt tttctttctc ggagctaaat gggttcaaga cctcttgttt
    43681 tggcggagca acatattcta caccaggaag caaagaacga taagtgtttt gagagcttcc
    43741 aatgtgtttg atcgcatcca gtatttttcc agtttctcga ttaactaaga cgatcgtact
    43801 atgacgtccc attaattcaa cgatcaatat gatattttgt agatcaccca actcgtttcg
    43861 tttagcaaag tggaaatgga tcactcgatc attttcgatt tgttcgatgt cttctaaaat
    43921 agcgccatcc aaatattttc gaagcatcat cacgaagtta ggcggattat caggattctg
    43981 ataatcgatt tgggtaatct gtacacgagc ataacttggg tgcgcagata ataataatcg
    44041 atggtttttg ccgcgtgaac gaatcaccag cacgatttca ttttcataag gctgatggat
    44101 tttcgagatc cggccagata gtaacgtttc acgcaattcg ttgaccatcg cgtgggtaaa
    44161 tacaccatca aaggacatgg ttcattcctc atttcttttc ataagtatat tcattataac
    44221 gaaaaaaaga agaaaaacca atttggccga atagtaaagc aaaagtctag aaaaatcttg
    44281 gactaattac ataaaaaaca gtgtttctat cgttgaaatc caaattttta
//
LOCUS       DACCSK010000008        42656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCSK010000008 DACCSK010000000
VERSION     DACCSK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.008"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1..771)
                     /locus_tag="IVP39_000402"
     CDS             complement(1..771)
                     /locus_tag="IVP39_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585505.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(764..1699)
                     /locus_tag="IVP39_000403"
     CDS             complement(764..1699)
                     /locus_tag="IVP39_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7585506.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2015..3664)
                     /locus_tag="IVP39_000404"
     CDS             complement(2015..3664)
                     /locus_tag="IVP39_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7585507.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAASFDEKRQWHVLG"
     gene            complement(3859..4755)
                     /locus_tag="IVP39_000405"
     CDS             complement(3859..4755)
                     /locus_tag="IVP39_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7585508.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNRWVDENEQKGQVFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(4748..5428)
                     /locus_tag="IVP39_000406"
     CDS             complement(4748..5428)
                     /locus_tag="IVP39_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7585509.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILMDKESRDVNVNGVTVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(5429..6211)
                     /locus_tag="IVP39_000407"
     CDS             complement(5429..6211)
                     /locus_tag="IVP39_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7585510.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(6204..7121)
                     /locus_tag="IVP39_000408"
     CDS             complement(6204..7121)
                     /locus_tag="IVP39_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7585511.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(7124..7339)
                     /locus_tag="IVP39_000409"
     CDS             complement(7124..7339)
                     /locus_tag="IVP39_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585512.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWQTTQPRLRDSLIFLEIQLE
                     NLYFLYTFLAAFFIPVNYTSLINKKEG"
     gene            8195..11422
                     /gene="fss3"
                     /locus_tag="IVP39_000410"
     CDS             8195..11422
                     /gene="fss3"
                     /locus_tag="IVP39_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7585513.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            11534..11848
                     /locus_tag="IVP39_000411"
     CDS             11534..11848
                     /locus_tag="IVP39_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7585514.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            11861..12235
                     /locus_tag="IVP39_000412"
     CDS             11861..12235
                     /locus_tag="IVP39_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7585515.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            12245..12589
                     /locus_tag="IVP39_000413"
     CDS             12245..12589
                     /locus_tag="IVP39_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7585516.1"
                     /translation="MEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            12658..13674
                     /locus_tag="IVP39_000414"
     CDS             12658..13674
                     /locus_tag="IVP39_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7585517.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            14268..16142
                     /gene="ltrA"
                     /locus_tag="IVP39_000415"
     CDS             14268..16142
                     /gene="ltrA"
                     /locus_tag="IVP39_000415"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7585518.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            16268..16624
                     /locus_tag="IVP39_000416"
     CDS             16268..16624
                     /locus_tag="IVP39_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7585519.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            16669..17811
                     /gene="dcm"
                     /locus_tag="IVP39_000417"
     CDS             16669..17811
                     /gene="dcm"
                     /locus_tag="IVP39_000417"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7585520.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            17801..18090
                     /locus_tag="IVP39_000418"
                     /pseudo
     CDS             17801..18090
                     /locus_tag="IVP39_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18346..19530
                     /locus_tag="IVP39_000419"
     CDS             18346..19530
                     /locus_tag="IVP39_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7585521.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            19545..19640
                     /locus_tag="IVP39_000420"
     CDS             19545..19640
                     /locus_tag="IVP39_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7585522.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            20410..22320
                     /gene="ltrA"
                     /locus_tag="IVP39_000421"
     CDS             20410..22320
                     /gene="ltrA"
                     /locus_tag="IVP39_000421"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7585523.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESSEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            22424..22648
                     /locus_tag="IVP39_000422"
     CDS             22424..22648
                     /locus_tag="IVP39_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585524.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            22661..23164
                     /locus_tag="IVP39_000423"
     CDS             22661..23164
                     /locus_tag="IVP39_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7585525.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            23291..24328
                     /locus_tag="IVP39_000424"
     CDS             23291..24328
                     /locus_tag="IVP39_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585526.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            24343..26040
                     /locus_tag="IVP39_000425"
     CDS             24343..26040
                     /locus_tag="IVP39_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP7585527.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            26033..26494
                     /locus_tag="IVP39_000426"
     CDS             26033..26494
                     /locus_tag="IVP39_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585528.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            26507..27367
                     /locus_tag="IVP39_000427"
     CDS             26507..27367
                     /locus_tag="IVP39_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585529.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            27405..27794
                     /locus_tag="IVP39_000428"
     CDS             27405..27794
                     /locus_tag="IVP39_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7585530.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            27781..30228
                     /locus_tag="IVP39_000429"
     CDS             27781..30228
                     /locus_tag="IVP39_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7585531.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            30233..32341
                     /locus_tag="IVP39_000430"
     CDS             30233..32341
                     /locus_tag="IVP39_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7585532.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            32338..33342
                     /locus_tag="IVP39_000431"
     CDS             32338..33342
                     /locus_tag="IVP39_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7585533.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            33361..34272
                     /locus_tag="IVP39_000432"
     CDS             33361..34272
                     /locus_tag="IVP39_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7585534.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            34459..34746
                     /locus_tag="IVP39_000433"
                     /pseudo
     CDS             34459..34746
                     /locus_tag="IVP39_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(34934..35149)
                     /locus_tag="IVP39_000434"
     CDS             complement(34934..35149)
                     /locus_tag="IVP39_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585535.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(35408..35575)
                     /locus_tag="IVP39_000435"
     CDS             complement(35408..35575)
                     /locus_tag="IVP39_000435"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7585536.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(35920..37359)
                     /locus_tag="IVP39_000436"
                     /pseudo
     CDS             complement(35920..37359)
                     /locus_tag="IVP39_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(37907..38578)
                     /locus_tag="IVP39_000437"
     CDS             complement(37907..38578)
                     /locus_tag="IVP39_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7585537.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(38927..40378)
                     /locus_tag="IVP39_000438"
     CDS             complement(38927..40378)
                     /locus_tag="IVP39_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7585538.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(40490..41833)
                     /locus_tag="IVP39_000439"
     CDS             complement(40490..41833)
                     /locus_tag="IVP39_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7585539.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(41967..>42656)
                     /locus_tag="IVP39_000440"
     CDS             complement(41967..>42656)
                     /locus_tag="IVP39_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7585540.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT        13917 a         7613 c         8300 g        12826 t
ORIGIN      
        1 ctaacttctc aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac
       61 gatgaataac acaagtacga tcatcgatgt tgctactggc acatcgatcg ttccaaggaa
      121 tccgtaacgg aaaccggaga tcatataaac gatcggattg actttcgaaa cagcttgcca
      181 aaagggcggt aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa
      241 gacaaatgta ggtacgatcg atacatcatc ataagactga gcaaaaatcc cgttgagcag
      301 tcctgctaac gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga
      361 atacacatga agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat
      421 actgcgaccc acaccaccaa tcacaaagcc ccaaataatc acatgcgtag gaacaggtgc
      481 taccaagatt tcttcgatat ttttctgaaa tttttgagag aagaaggacg aagaaacatt
      541 ggcatacgaa ctagtgatag cagacatcat aatcaaacca ggaacgatga attccatata
      601 tgaaaaacca cccatgtcac cgatccgccc accgatcatt tttccgaaaa taacaaaata
      661 caaggatgtc gtgatcactg gcggaaccag tgtttggacc catatacgca aatagcggtt
      721 cgtttccttg atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct
      781 ccgtatgatt attttcttct gtgattttca agaacagttc ttctaaacga ttcgacttat
      841 tcctcataga aagaactttg attccttgtc ttgtaagctg atcgaagatt ccgttcaccc
      901 cttgatttcg ttcaacttcg acagcgattg tctgctcatc ttcaaatgta caagcatatc
      961 cttcaatcac aggtttggta tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca
     1021 gttttgacag aagctctttc atacttgtat tctcgatcag ttcacccgat tggatgattc
     1081 cgatgttgcg acaaagcatt tcagcttctt ctagataatg cgtcgtcaaa atgatcgttg
     1141 taccattttc gttcaattct cgtagaaact cccacatctc gcgtctcaac tcgatgtcaa
     1201 ctcctgctgt tggttcatct aaaatcagca aacgtggttc atgcatcagt gctctagcaa
     1261 tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc
     1321 aaagattgga ctgcttcaaa tatttttcac tgcgtttcat cgcttctttg cgagaaacac
     1381 cgtagtaacc ggcttgattc acgacgattt gctggaccgt ttcaaaaggg ttaaaattga
     1441 actcttgtgg aaccaatcca atctgttgct ttgctcgaac tagatccttg tccagatcat
     1501 aaccgaagac attcactttt ccagatgttt tattgactaa agaagtaata atcccgatcg
     1561 ttgtagactt cccagctcca tttggcccta atagcgcata gaaatctcct tctttcaccg
     1621 aaagatcgat tccccgcaat gcttcgaccc cacttccata gatctttttc aaattattta
     1681 tttcaagcgc atttgccatt tatttcctat ctccttatag acatgattct ttattgtatc
     1741 atggaataca gcagatgaaa aaacaacttg gttccagcga aaaatcaggt tgctgggctc
     1801 aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc agacaacctt ttcagaaaaa
     1861 acaaatcgaa gataaaggat tactattcat atgtgcaaca aaaggagcgt gagacaaaag
     1921 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca tgagccaaga
     1981 tttttgatcc aataaatact ttcgatcact tcgcctatcc caatacatgc cactgccgct
     2041 tttcatcaaa tgacgcggct tccgtttttg cggtttctac taaagtctct gggtaatcca
     2101 aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt aatttgaaat
     2161 caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc tcacaataat
     2221 tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa gatggatact
     2281 ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta tttgttcctt
     2341 ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg tccagcaaac
     2401 gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga
     2461 ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt
     2521 ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta gattttcctg
     2581 atgcattgga acccgtgact aatgtattct tctgccagtt gactggattc actaccgctt
     2641 cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta aactctggtc
     2701 ggcaagtgat cggcatatag gtgcgataat tcaaaacggc tgctgcaact tccaattctc
     2761 ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta gtgagaacga
     2821 aatgatagga gataaatggt agcatgaaca tgatgttgac atattcaaaa atcaattctg
     2881 cttcactccc gctcttcatt cgaaatgaaa aacctaattt agggatgtct cttaatggtt
     2941 tagtttgctg tttcagctct ttttgaagtg gtgtcgtcat ttttgctaac tgattggcgg
     3001 ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt gtttgtttct
     3061 tcactagata atagaccgta ttgaatacta agccaccgat tgctagatag attcctagta
     3121 cttgacctaa aagaagcaaa cctacaccca ccagcggaag caagccaaga aaaataaata
     3181 aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt gcaaaattat
     3241 gatctttttt tcctaaacga gcgaattgat attggattct ttctctcagt tgaggatttt
     3301 cctgataaaa agcaattagt ttttctaact gataatcttc tccaaaatca aagctacgta
     3361 aacgctgata caacgcttct gaacctacac tagaataggt actgttgatc ccttcaaata
     3421 cttcgaacat atccaagtca taccaagtaa gatcgtcgat ttcactatcc catgaacgga
     3481 attttttctc cgtgagccac gcatccttca aactttcttc tttatcaaat ctcggttgat
     3541 aaggaatttt tccccaatta ctacgaactg tttcctttac tttcaaacgt gaaaaaatgt
     3601 ctaaaacaac taataaaata acaactgcaa taataattaa aacgataaat tctctttggc
     3661 ccatatccct ctccgttcct tgatttgtta gttttagtat agcactgttg cgctatttga
     3721 aaagggggta caaaaataac tatcttcgtt acctaattcg tatcaatcac accaaaataa
     3781 aaacaccttc caaaaaagaa gatgtcttta aaacgagata caattttttg tcattcactt
     3841 caggtaaatt agagccaatt aaaccctccg taacttgacg gagatggaga agatgccccc
     3901 gtctaatcta gcggtcatct ctcctcccat tccttccacc aatttttttg acaggtaaag
     3961 cccgattccg ctagattccg tatttgtgcg gctggtattt tcggtataaa aacgctggaa
     4021 gactttttca acagaggttt gcgctcctgg tttgatagga ttggccactg ttaatcgaat
     4081 gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca tctgcataac gcaataaatt
     4141 ctgtatgata ttttgcagca ctcgcagcaa catgagcgga tctgctaata cagttgtatt
     4201 tatcgagtgt tcagcgaacg ctacctcgat atttttctca gaaaattgtt cataaaaaga
     4261 gaaaaatact tcttccacct ctttagaaaa agaaagcgta tggagatcca tttctttttg
     4321 cttcgtctct attcgagcaa gatcataaaa ctgatccgtc atactgagta atcttgcaac
     4381 agattgtttg atgatcgtta aatacttctc ccgtttctcc gtatcctcta cctgttccag
     4441 taattgtaca taaccgttaa tagcagtcaa aggagttctg agatcatgag aaaggccgac
     4501 gatcgatagt ctgatgtttt cttccatttc ttgaaatact tgtccttttt gttcattttc
     4561 atccacccat ctattcagtg catctactag gtccattaga ttcttttctc gaaattcgac
     4621 tgaaagacga ccaccatatt cttgtcgttc agaaagagtg gcgattttct tagttaatcg
     4681 cttgatttcc ctttgcagga agaaaataaa acttcccata aagataacaa cgagaagcaa
     4741 cagccactta atcaaatcta aaccccactc cccacaccgt atgaatatac acagcatgtg
     4801 cctttgctag cttccctcga agattggaaa tatgcatatt cactgttttc tcactatcaa
     4861 aatatggttc ttgccaaact gtttcataaa gatttgctcg cgaaaatact tttttcggat
     4921 gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt caaatggaca gtcacaccat
     4981 tcacatttac atcccttgac tctttgtcca ttaagatttc cttgtaatag atgcgttgcg
     5041 acagatcttt tacttggaca gaagtctgat gacgaagttg gatgccgatt cgtaacaaca
     5101 gttcttcgat atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa
     5161 ctttatctct ttgatcgctt tttgctgaaa gaacgatgat cggaacattc gatgtctgtc
     5221 ggatttctgc taacaatgct tcacctgaaa gccctggaag catgagatct agaaggatca
     5281 aatcgacagg atatgtgctt aacaataatt ttccttccgt acctgaataa gcatcaagaa
     5341 gtttgtatct ctgttcaaga acctcctcca gtaaagaatg aacagcttca tcgtcttcga
     5401 taatcaaaat agtttccagc aggaccacct acttaatgtc agatttttgg aaaataaaca
     5461 ttcctagagc tgatgatacc acgattgtta ctagactgac cagaatcatt ttcggtgctt
     5521 gcgaataagt tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc gtaaaccact
     5581 ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc gggaacgaag gccgttccca
     5641 tgatataaag aaaagaaaga agcaaactga cactggtact tctagtgatg aatgtcaaag
     5701 ccactggaac agataatgtc gctaatatca gcagaagcaa tacaggaaat acttttagaa
     5761 cataatttcc atagtttttg attccctcaa cttcaccaaa tccgaaagca agtgtgtaag
     5821 caatcatggc tagccccgta atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga
     5881 gaagtttcga cacataataa gtcattcgat tccgtcctaa agataacgta ttgcgaatcg
     5941 taccgttttg aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa
     6001 tccatgcagc cattttttcc accattaaaa tccctgttgc accgttgatg actgtatccg
     6061 ttgcttggtt taattcattg ccgtattttg tatgcatata ccattcgttc caagaataaa
     6121 aaagcggaag tacgaccatc agtcctagtg agacccacat tttacggctc ttccatactt
     6181 ttattttttc agcagttaat agattaaaca tgtggattgc ctccagttaa ttgtaagaaa
     6241 taatcttcca gtttttgccc aactaggtgg ataccatcca caggaacttt tgcctctacc
     6301 aatgcttgat tgatggctgc tacttggtca attccttcaa aaagctggat aacacggcta
     6361 gattggacaa aataatccct ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc
     6421 gccgaagtgt caatttttat aaattgacga gattcttgtg ccaattcttc tttcgataat
     6481 tctttgatca agtgtccttc atgcaaaata ccgtagtgag tggcgatctg tgacaattca
     6541 tctagcaagt ggctagaaag caagacggtg atgccccgtt cttttaccaa acgttggatg
     6601 atttctcgca tttctgccat ccctgctgga tccatcccat tcgtcggttc atccaaaatc
     6661 aaaaattctg gattcgtgat cagtgcattt gcaatagcta aacgctggcg catacctaaa
     6721 gagaaattct ttgctttctt tttccctgta ttttccaatc gcatcatttt cagcaggtct
     6781 tcgatttctc tatccgacac accgccgttc gctgcttgga tacgtaagtt atctcttgca
     6841 gttaattctg gataaagagc aggggtttca atcgattgac ccatacgtcg tcttgctgcc
     6901 tgcaaatccg tcgttccaaa taattcgatc gaaccttcat caatcgagat caaacccata
     6961 atagttctca taaaggtaga cttacccgct ccattctccc caatcaaacc atagatcatt
     7021 ccacgtttga tttcgatcga tactttatct aatgctttgg cagcaccgta ttttttggtt
     7081 ataccagttg ctttcaagac cgtttcattt ccaaaatcca tacttatcct tctttcttat
     7141 tgataagtga agtatagttg acgggtataa agaaagcagc cagaaatgta taaagaaagt
     7201 ataaattttc caattgaatt tcaagaaaaa tcagactgtc tcgcagacgt ggttgagttg
     7261 tctgccaatc aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa
     7321 tcgttgttat cgttgacatc agacgtcctt gtatatgcgt tggaacttct cgctgaagct
     7381 agaccatata aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat
     7441 acatgccgtt tgggatttac aattagctac caaataagag gttatgacaa aagacttttc
     7501 acaatccctt cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     7561 tcttcatcaa acatgagaag cgatttttgt aaattccttc ttatttcttg aagctgacca
     7621 cttctgtcac aacctcagaa gcggataaat aatattgttt gaagatactt gatctttttc
     7681 ccgtttgttt atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta
     7741 aaaacacaaa aaggcacttt ccaaattgtg gaaagtgcca gaaaagttag gattaaggta
     7801 actgcgctca gcattgcttc gcccagtacg attctcgtaa ctcgtgaact cgaacgataa
     7861 tcgcaattaa cgttttgaga actgtgaagc tttacgggct ttcttaagac ctggtttttt
     7921 cttcgtatca ttgaataagt ttgaaaagcc ttgatttata aaggtttttc cgtaaatata
     7981 ttttgttata tttgattaaa tttgaaatgt ttgtgttcta aaatgtgttc taatttaaag
     8041 gcagtatatt tcgtatttta tattaaattt ttaggctcat tcattctcaa atccattcta
     8101 tttagtcaat aaatatttta aaaagacttt ataaacaaag atgttcaagc gagcatctta
     8161 tttttttgtg cctaatttga aaggagaaca tttcatgtat aaaaaacaaa aaaagaaatt
     8221 atgtgtatta actattttga gtttggctgt tcaatcaatt gtaggagcaa ttgctcctac
     8281 gattgctttt gctgatgaaa ttacccatcc acaaacggta accgtagaac tagatttagc
     8341 acatcaatat gctgttgaag gaacttttag tgatggtcgt ccaatgtcag aagtcactgt
     8401 tccgcattat gcagtgtaca acggcgttaa acaagatatt ttctgtgttg aaccaggagt
     8461 cccgatttac aatgagttca ctcctggata tgagaagaac ccgttaccag atatgtccga
     8521 taaagccaaa ttagtttccg ttttatggaa aaaagtaggt acagatgcag atactcatat
     8581 tgtggcacag aagatgatct ggcaagaagt taatgggtat acccttcact caataaaacg
     8641 atcagatggt agtgccgtaa atatcgcagc cattgaggca aaaatcaatc aagccattgc
     8701 cgattatcag aagaaaccaa gcttccataa tagtacggct aaaacagtat tagggcaatc
     8761 gactactgtg acggatacga ataatctgaa tttgtcagag ttcgatgagg ttgtggaaaa
     8821 taccgcaaac attgattatc gtgtgaatgg caatcagttg ataatcagcc caaatgccaa
     8881 ttcaaaagaa agtggtgtgt tgacgcttaa gaaatctgct ggtaccggaa cacctgtcgc
     8941 ctataaaaag gctggccaac agactttaat ggccggggcg atcgataagc cgaacactta
     9001 tacggtcaaa attgatgtgg aaatggaggg ttctttgaag attaaaaaag ttgataaaga
     9061 atcgggtgat attataccag gaacggtttt ccatttagac tttggaaaaa ctttacccgc
     9121 aaaagacgtg accaccgata aagaaggcat tgcgacctta gatgagattc ctcatggtac
     9181 aaaggtaacc attactgaaa aatcggtgcc ggcgccttat acgattgata ccacacccat
     9241 gactgccaca attaaagcag gtgaaaccat ttcagtgact tcaaaaaata cgcgagaaaa
     9301 aggtcaaatt attctggata aaaccggaat tgaaacaggg agtgatcttt ggaacgataa
     9361 ttactcttta gcgggcaata cgtttgccat tcgcaaggat agcccaactg gtgaaatcgt
     9421 ccaagaaatg accacggatg aaaaaggtca tgcagaaaca ccaaaagaga ttgccaatgc
     9481 gttggaattg ggaacctact atgtgactga aaccaaggct agtcatggct ttgtgaatac
     9541 tttcaaacca gtaaaagtcg aattgaatta tgtcaatcaa acagtggctc ttgttaccag
     9601 taacgtaaaa gggcaaaacc aagaaattac tggtgaaacc actttgacaa aagaagacaa
     9661 agataccggt catgaaactc aagggaaagc tgagtttaaa agagctgaat acaccttgtt
     9721 tactgttaag gatggaaaag cggtcaaatg gaccgagaac tttaaaccag aattgttgaa
     9781 gggaacgaaa gcttctgatg aaaccgtaac tttgacttta gatgaaaaga atcaagttgc
     9841 cgttaaacac ttagcgatta atgagtatta ctggcaagaa accaaagcgc ctgaaggata
     9901 taccttggat gaaacaaagt atcctgtatc catcaaaaag gtggatgata gcgaaaaaaa
     9961 tgctgtgatt actcgagatg ttgcggcaaa agaacaagtg attcgctttg gctttgattt
    10021 ctttaaattt gctggatcaa ctactggcac tgccgaaact ggcttcaatg atctaacctt
    10081 taaagtgtcg ccattggaag gaaccaatga aatcaccggt gccgaagatg aagcgaccac
    10141 agcttataac gagcaattag ggtttgatgg ttatggcaag tttgaaaatc ttccttatgg
    10201 ggattattta cttgaagaag tagaagcccc agaaggtttt caaaagatta cgccattaga
    10261 aatccgttct acttttaaag aaaataaaga ggattatacc aagagtgatt atatctttac
    10321 tattaccgaa aaagggcaaa agcaaccgat taaaacggta acggtccctt atgggaatct
    10381 aaccaatgag aatttctcag tcagtttgaa ccgtttgatg ctttatgatt tgcctgagga
    10441 agaagatagc ttaacttctc ttgcgacttg gaaagaagga gataaagaat tgactactct
    10501 tgattctacc gagctagtcg ataaattgag ctataatcta catgaaatca aagaagactg
    10561 gtatatcgta gctcaagcca tcgatgtaga tgctacaaaa gctgcccaag aaaaagataa
    10621 aaaagccaaa ccggtggcga ttgcggaaac aaccgcaact ttagcgaata aagagaaaac
    10681 aggaacttgg gagattctgc ataaattaac tgtcgaacaa gttttagaca agaccattgt
    10741 cttattcaac tatgtgtatg aaagcaaaga agcctatgaa gctggtgatg agccggtagc
    10801 gaaggatgct agcttgaaca atcaagcgca aactgttaat tgtatggtgg aacgtcatgt
    10861 ttccatccaa accaaagcgc acttagaaga aggttctcag acctttactc atggtgacgt
    10921 ggtagatatg tttgatgatg tatccattac ccatgatgta ctagatggct caaaagaagc
    10981 ttttgaaaca attctgtacg ctttatttcc agatggtaca agtaaagaaa tttggaaatc
    11041 tggcaagatt gattacgaag tgaatgacaa agaatttacc aaaaccgtac ttgcggaaaa
    11101 agtggataca agtaaatatc cagaaggtac aagttttacc ttcaaagaaa ttaactatga
    11161 caaagatggg aatatcaatg gaaaacacaa tgaagatttg aaagaaaaat ctcaaacttt
    11221 aacgcccaaa aaagttccga caacaccaag tacaccagaa caatctgaaa tgccaactgt
    11281 tccaagtggc tctcaagaat ctagtcctac agtgaaggca ttccctcaaa ccggggagaa
    11341 aacttccaat gtgctactgt tcattggctt tagcttgatc tttgcgacgg ctggctatta
    11401 tttttggaat cgtcggaact aaggtgataa gatgaaacat gtaaatagta gaaaacaata
    11461 cgcgcgaccg cctcctaaaa aagtggtcgc gctatttttg tattcaaaac taacaatgaa
    11521 aagaggaaaa aatatggaat tgaaatttgt tgtaccagat atggtcgaga cgttcggaaa
    11581 actaacatac ggtggtgaag gtgaagttct atcggaaggg tatggtcgaa atactacaat
    11641 tgttggtcga agttaccatc tgttttcaaa taaacaacgg gccgatgata ttgaagtggt
    11701 ggtagctgcg gaagccggcg aaaaggattt tgaccaagat caaccactaa aagctgtaaa
    11761 tccccactta attgccaaag gctatgaaat tgagaatcgt gggtttaccg attacgtgtt
    11821 gtatgttgat gatttagtga aagcatagga ggaaaagaag atgagattaa cagaaggtat
    11881 tgtcgtagat tcaggattaa cctttggcaa gttgcgtttt tccgcattac gtcgagaagt
    11941 acgcaagcaa aatgaggacg gaacggttag taacgaggta aaagaacgga cctataattt
    12001 gaaatcctcc gcgcaaggtc gaatgatcca agtcagtatt ccagcaaatg tacccttacg
    12061 cgagtttgct tatgatgcag aggtagagct ggtcaatcca atcgtggata cggtcgccaa
    12121 ttatgttttt cgggaaggaa ccaccgttaa ttggtttatc aaggcagatg atttagtctt
    12181 gaaacgacaa ctgaatcaag gaaaacctac gaaccaaaat gaaggaaaga aataggtgaa
    12241 gaatatggaa attaatgttg aacatatttt agattgtctc gatcaatttg gcaaaggtga
    12301 gctaacgggg gaacaactca ctaacgcatt aacctatgat gaaaaaatgt ttctgattat
    12361 gcatcaaggg ttactggaag taggtaacga tgccaaagaa gactttgatg tactggattg
    12421 gttaggggaa gaagagtctt tctttatggt tgtcgaggta aatgaaagtt tatgtcggca
    12481 ggcagaatct gttttggaag aaatcgatgt ggagatgccg gatgccatcg aaagattttt
    12541 aaaacaatta gtggaaacga aacaacttcc cgtagtagta aatgactagc ataacttttt
    12601 gaaattgccc aatgaaatcc tattgggctt tttcgactgg aggtggaaac catcaaaatg
    12661 tacaaaggac atcgcatacg cgcaggagat caacacttga tttatcactt tgttcttggc
    12721 tggctaatct ttttgttcat tggttggatg agcgtctttt atttacaaga acttagacaa
    12781 tttgatattt ctaaggtatc attttccact agtgaaattg gctggtctat gaaggatgta
    12841 atctgcttgc taggaagtct cgctctttca ggaagcatga tgttacttta tatacacttt
    12901 tttcaggatc attggcgaag tctttggcat cgacaaaagt tagctcggat gattctagaa
    12961 aaccattggt atgaagtaaa gcagactcaa ggtgaaggtt ttttcaaaga tttgaatagt
    13021 agttggacca aagagagtat cagttacttc cccaaaattt attatcgaat gaaagatggc
    13081 ttactttcta ttcgcgttca aatttcactg ggaaaatatc aggatcagct attgaagttg
    13141 gaaaataagt tagaaagcgg actgtattgt gagttagtag aaaaagaact caaagactcc
    13201 tatgtggaat acaccttgtt atacgatatg attgccaatc gaattgggat agaagaaata
    13261 gtggcagaaa atggggcgtt gcgattgatg aaaaatcaag tatgggcgta tgattccttg
    13321 ccgcatatgt taattgctgg cggcacaggt ggtgggaaga cctatttctt actcaccatt
    13381 attcaagcac tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa aaatgcggac
    13441 ctcgccgatt taggtacgat aatgcctcac gtatactccc aaaaagaaga aatttctgaa
    13501 tgtgtggaag atttttacga acgcatgata gctcgtagta ggtcaatgaa agaaatgccc
    13561 aactacaaaa caggagagaa ttatgcgtat ctcggacttc cgcccaactt tttaatcttt
    13621 gatgaatacg tggcttatat gggtgcgaat cgttttccta caagcatcga atagatgtga
    13681 aatggaagcc tattgagatt atcaatagtg acaactaatg ttttgagaga tggaatgaaa
    13741 gccttcaagt tgagctgaaa catccccgct aatactccta catgctctaa tcaatgaagt
    13801 agattggttt tggtatgaag ctaggtgaag tcgtctgaaa tctgcctaag tgcttgagca
    13861 taaggctgac tatagggcgg gcggctgaaa aagataacta tggtaagaat gtctgaaaat
    13921 gactgacgaa tctgcgaatg tacgggtcta aaaggctgat cttattgaaa gataggaaca
    13981 tacggacgta tggtgagata ccgacaagta aggttccatg atatgaaaat cgggatagct
    14041 aacgaagaag ctataatttc acaggctcat agtagatacc taaggttata catgatagct
    14101 aaaaacattg gaacgatgta agcagaaaac tatcaattaa atgactactt ctagataggt
    14161 gcatataagt aataacgaaa ttgctttgtt tctgtgaaag taagggcaca gtaccgatga
    14221 agcgtctaac aaatgtggag ggatagccct aagtctattt aataacaatg aatgttgaaa
    14281 cagatgaagt tcaaggatct ggtaagaacg tgggaaaatc cgttatggag gtgccacgct
    14341 tgaacacaaa aatacgttac tgggaatatt atgatctaca agaaacattc gatcagctat
    14401 ttactcaaag tagaaacggg aaaaagtttt atcaattata tgagttaatc atttctgaaa
    14461 ataatattct tttagcatac cgtactatca aggcaaacaa ggggtcttcg acaccaggaa
    14521 cggattcctt cacaattgat aactataagg aaatgaacca agcagagttt atccatctta
    14581 ttctcagtca tttagaaaat tacaaaccaa aatccataaa gcgagtaatg atacctaaac
    14641 ccaatggtga gaaacgccca ttaggaattc catgcatgat tgatcgaatg atccaacaga
    14701 tgttcaagca gatactggag ccaatatgtg aagctaagtt ttatgaacat agctacggtt
    14761 tcagaccatt gagaagtgca aagcatgctc taggtcgcat tatgtatctc ataaacatca
    14821 gtaaaatgca ctatgcagta gacattgata tcaaaggttt ctttgataat gtaaaccacc
    14881 gtttactgat aaaacaatta tggaatatag gaatttgtga caaacaagtc ttagctattc
    14941 tttcaaaatc tctgaaatca cccattcaag gagaaggtat cccgagcaaa ggaacgatcc
    15001 aaggaggtat catttctcca ctattatcta atgttgttct aaacgactta gaccattggg
    15061 tctcaaagca gtggcatacc tttgaaacaa agtatcccta tactaaaggt tacaataaat
    15121 ttcgtgctct aagagataca aatctaaagc aaggctatat tgtcaggtac gcagatgact
    15181 ttaaaatcat gactaatgat tatccgacag cattaaaatg gttccacgct gtcaagcttt
    15241 acctaaaaga cagacttaaa ttagatattt ccaatgagaa gtctaaaatc gtcaatttgc
    15301 gtaaatgtaa gtcggaattt ttaggattcg ctatttgcgt gaaacaaaaa gggaaaaaat
    15361 gggtttgtaa ttcacgtatt tctaacaaaa aaaaggatca gattaaagaa gagattaaac
    15421 aaagaatcaa agatattcaa aagagtccta cagcacaaaa tgctctcctg tttaatgctc
    15481 tcgtgctcgg tgttcataat tatttcaggt atgccactca tattaattta gatttgaata
    15541 ggatgaacta cgttctttca ttcactactt acaaccgatt atcaaattgt agtacaagat
    15601 gttaccccaa caaagcttcc ccaacttaca aaaagtttta ctcaacaacg atgaaaacgt
    15661 ataaagtagc tggagtctat ttgtaccctc tttgcgacgt atctacaaaa acaatttacg
    15721 gatttaatac ggaggataca ccgtttactc cgtttggtcg tcaaagattg gagcacaaaa
    15781 gcctacagtc tcttgtgttc caagaattaa gaaaattgat ggaatcgaaa ataccgaata
    15841 gaacggttga gtatttagat agtagaatat ctcgttactc aatgaaatca ggcaagtgtg
    15901 agattacaaa acaatttttg ccagcaaaag ccgttcactg tcatcactat ttgccaaaaa
    15961 gtcttggtgg agacgacaaa ttcgataact tgagaattat tcataaggat atccatcttc
    16021 tgatccatac aacaaataaa atgataatcg atcactacgt gcatgaactg aaactggtgc
    16081 cagagcaaat agcaaaaatc aacctttatc gaaagatgtg taatctgcaa aacattcaat
    16141 agaaaataaa tagatggaac gccgtatgag gtgaaagtct cacgtacggt gtggagcggg
    16201 ggaaaagatg gagattatat caaattctta cctatcgcta tagatgttaa cgacaaaaga
    16261 aagtgcggtg attttgaata agcttaaaca aatcgtgatg ttaggccgtc aatctggttt
    16321 ctttctgatt ctagcttgtc aaaggccgga tgcgaaatat ctaggagatg ggattcgcga
    16381 tcagtttaac ttccgtgtgg ctttgggtcg tatgagtgag cttgggtatt ccatgatgtt
    16441 tggagaagtc gataaaaatt tttttatgaa acgcatcaaa ggacgaggtt atgtagatac
    16501 agggggcagt gtgattagtg agttttatac gccacttgta ccaaaagggt atgatttctt
    16561 aagggaaatt agcaacattg ttggattaac cgttcatacg gaaagagaaa ataatagtgt
    16621 gtagcctagg aatatcaata atcctgggct tttttattga ggtgatttat gacattttta
    16681 gatttattcg ccgggattgg tggctttcgc ttaggtatgg aacaagccgg tcaccagtgt
    16741 atcggttttt gtgaaattga tgaatttgct cggaggagtt ataaagcaat ccatgataca
    16801 aggaaggagg tggaaatgca tgacatcaca agtgtatcag acgaatttgt tcgatccctc
    16861 ggaccagtgg acatcctttg cggcggcttt ccgtgccaag ctttttcaat tgcgggaaag
    16921 cggcaaggat tttccgatac tcgaggtact ttattctttg aaatcgctcg gttcgccact
    16981 cttctccaac ctaagtttct attccttgag aacgtccggg gattgctcaa tcacgaagga
    17041 ggggctacgt tcgagacgat cctccgaacg ttggatggat tggggtatga tgtggaatgg
    17101 caagtgctta actcaaaggc ctacgttccc caaagccgtg agcggatctt ccttatcgga
    17161 cattctcgag atgcatgtac cgaacaagta tttcctatca ttggatcgtc ttcaacatct
    17221 gatcaaaaca tcagaaactt ggtaaatatc aatccttcca atcgaggaat gggtggccaa
    17281 gtctatggtt cagatagcgt agcaccaacc ttgaccggtg atgaaggaat taaaattgcc
    17341 ttaccggtaa aggatgggat ttctgtagcg ggtatgttgt cgggaaattt tgaacaaggc
    17401 aataggattt atgaggtaac cggaacagcc cccactttgt caacgaaaca agggggaacg
    17461 aagattatga ttcggaaaaa tgaaacagaa tatcaagagg tgaagccagg ggacagtgtg
    17521 aacctggctt ttcctaattc cacaagtcgc cgaggtcgtc tgggaaaaca aagtgtgcat
    17581 accttactaa caggagacca acaagcggta gtcacggatc agtaccagat tcgaaagtta
    17641 acacctaggg agtgctggcg gcttcagggc tttccagatt gggcttttga tcgagcttct
    17701 caagtcaatt cagatagtca gttgtataaa caggccggaa actcagtaac agttccggtc
    17761 atttttgata ttgctagaag attaaagggg gtaaaagcat atgatttatg acgagttgat
    17821 tggagaaatc tactgggtga ttggcaagat tcaggcagat ccgaaattgg aagaagaact
    17881 gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa gtccaggtga ttcgtatgta
    17941 gtggatgagg aaacggatgc tcgaatggag ctcaatcaag tcatcggtga attggaacga
    18001 attgcggacc ttgccaaaga acctgatatc cgccaatact tgtttgagat aaaagcagag
    18061 ttggaaattg agggtatcac cgcggagtaa tagggaaata acccactgat aggcactgac
    18121 cgcgggggat ttgcgaagca gaaatcctcc gtggtgctgt gcctgttcat ggacggcggc
    18181 agccggccat gatgtctcac cccccgtatc taacaggggg gtacaaaaac acaaaaaaca
    18241 agcaagtaac cataaaaaat gcttgttgta tcaaggatat taagtaattt gtataggtgt
    18301 taactcattt ggaaaagtta acaccttgaa tttggaggtg aaaacttggc gcaacgaaat
    18361 ttagattatc ggttattaaa agaacgacgc aacgaatatg gcgtttcaca aaataaatta
    18421 gctactgctt gcggacttag ccgcccatac ctaaatcaga tagaaaacgg tggcgtaatc
    18481 gcctctacaa agaccatgag aaaaattttt gatcaactgg aaagtttcaa tcccgattta
    18541 cctttaacgt tactatttga ctatgtaagg attcgctttc ccacaacgga tgtgcggaaa
    18601 attattcaag aaattctcca tttgaaattc gagtatatgc ttcatgaaga ttacgccttt
    18661 tactcctatc aagaacaata tgtcatgggc gatattgtag tgatgttatc tcatgaagaa
    18721 gataaaggtg tccttttgga attaaaaggt cgtggttgcc ggcagtttga aactttttta
    18781 ctcgcccaaa agcgtagctg gtacgacttt tttgaagatt gtctaaaagc cggtggcgtg
    18841 atgaaacgct tggatttagc aatcaatgat cgagtggggc tactggatat tcctgattta
    18901 acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtaccttcaa aagttaccgt
    18961 tccggggaac tgttgaaagc tgatgaaaag gatggtatgg gcaatacctt atatattggc
    19021 agcctcaaaa gcgaggttta tttttgtttg tacgaaaaag attatgaaca atatatcaag
    19081 ttagggattc cattggacca gaccgaaacg aaaaatcgtt ttgagattcg gttgaaaaat
    19141 gaccgtgctt attatgcgat tcaagatttg ttaaaaggtc gtagcattga aagtaccacg
    19201 ttttccatta tcaatcggta tttgcgattt gcggataaag tagatggaaa acggcgaacc
    19261 aattggccct tgaatgaaca atggggtcgt tttattggac ggaatcggaa agagattcaa
    19321 ttaacttctg aaccgaaacc gtataccatt gaacgaacat taaattggct aggtagacaa
    19381 gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcaaac aacgtatatc
    19441 caggatatgg tacagaatgc gcgactctcg gaccggcata aaaagatttt ggagcaacaa
    19501 agtatggcaa tcgaaaactt gattgtatga aaagaggaat tttgatggaa attgtaaaag
    19561 atatgttatt ggtacttggt ggcagtatgc ttggtgttac cgtgatgtgc ttgatgaatg
    19621 caagtgcagc agcggattga gcaatggaaa agaaaggaaa gtgacaaaat gaatttcgga
    19681 caaaatttat gtgcgactcg ttccttagtg aaaagctaag gcactaataa aagaacgaaa
    19741 aagtttggtt attaggagaa ctgataatct gacaggtgga atgatgaagt aacgctctga
    19801 aacgcctgcc ctgagcctcc gacaagcatt gtatgaggaa actcagaatg tttgaagctc
    19861 ggtaaagacg gctgaaagat accgtaatag ttgatgaaca tatatcttct atcgaaaact
    19921 atccagaggt gggtagcgta tccaacaggt cgggggtcta taaaatatct atggttagaa
    19981 tgttctataa ggaactgacg aaacagcgaa tgtacgggtc taaatttgaa ctttgtagaa
    20041 atgcaaaggt acgagaaatc gtagctggtg tggcaaagta agaatatagg ttatgaaatg
    20101 ccatataacg ttacaggcgg tatcaagcca gcaggcttat agctagtgac ctaaggatat
    20161 atatgtatag ataagattat cggaacgagg aaaggtattg ggttctcatc aagagaataa
    20221 actgacgaag aacaataagc agttgaacta atgctgaaaa gcagagggcg aacctaagaa
    20281 attcttgtaa tgagaatgga ggaatgccct caagtcgtgt atatattaga aaagtattat
    20341 gttgtttcaa taaggattac gagtaagata aaaaagctca ctcaatttca agtaaaggag
    20401 tgaagcctta tgcctaaaaa taaaaatgaa acattgtgtg tagaagattt aagacacgct
    20461 gaatattacg aaatgcaaaa cacctttgat gatttatatg ccaaaagtaa gaatggagat
    20521 attttcactc atcttatgga tattatccta tcgagagaaa atatattact ggcatatcga
    20581 aatatcaaag caaatgcagg cagtaaaaca gcaggaacag acggtacaat aattaaagat
    20641 attggcaaat tgccagcaga aacagttgtc aaaaaagtaa gatacattgt tgcaggcagt
    20701 ccacatgggt atcgacctaa gccagtgagg cgaaaagaaa taccaaaacc aaatggtaaa
    20761 accagacctc taggtattcc ttgtatgtgg gacaggctta tacaacaatg tataaaacaa
    20821 gtattagaac caatctgcga agctaaattt agtgaaaaca gctatggttt tagacctaat
    20881 aggtcagttg aaaatgcaat aaaagcaacc tataaccgcc tacaaatcag ccagctacat
    20941 tatgtgattg aatttgatat taagggtttc tttgacaacg tgaaccattc caagctgata
    21001 aaacagatat gggctatggg aatacgggac aagcacttga tattcatttt aaaaaggata
    21061 ttaaaagctc caatcaaaat gactaacggt acaataacat atccagagaa aggaactccg
    21121 caagggggaa taatatcccc actactagca aacattgttc ttaatgagtt agaccattgg
    21181 gtagaaagcc aatggcagga aaatcctgtt acaaaaaact atgtggttca tatcaataaa
    21241 agcggtagcc catgtaaaag caacgcttac aaagaaatga agaaaacaaa gttaaaggaa
    21301 atgtatatgg ttcgttatgc agatgatttt agagttttct gccgctacaa ggaaagttct
    21361 gaaaaagcca aaatagcaat cactcaatgg attgaacaac gattaaaatt ggaagtatca
    21421 caagaaaaaa caagaattgt aaatgttaga aaaagatact ccgactttct tggtttcaag
    21481 attaaaatga taccgagaag aaagaagtta gttgttaaat cacatataag tgataaacaa
    21541 ttcaaaaacc aaaaagacaa gctggtatca caggcaaaga aaattgcttc tccctcaaaa
    21601 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
    21661 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
    21721 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
    21781 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
    21841 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
    21901 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
    21961 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
    22021 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
    22081 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
    22141 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
    22201 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
    22261 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
    22321 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
    22381 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
    22441 atccatatat caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt
    22501 cattggtttg tttttaggat ttaaacggga gttttccaaa ctgattggct tcttggtgat
    22561 tgctttgatt gcggtaggtc tggtctttaa tgcttctggt gtgaaggatg tcctactcaa
    22621 tttgtttaac cgaattgtcg gtgcctgagg gggaatcaca atggaacaga tgcgtgttta
    22681 tattgcgaac cttggaaaat acaatgaagg tgagttggtg ggggcatggt ttaccccacc
    22741 tgtcgatttt gatgaagtga aagaacagat tggattaaat gacgaatatg aagagtacgc
    22801 gattcatgat tatgaactgc cttttgagat tgatgagtat acgccaattg aagagattaa
    22861 tcggttgtgt aacttagcgg aggaactaga aggaacgcca attggtgaag ctgcttctga
    22921 gattcaacac gctttcttca attctttcga ggaaatggta gagcatgtgg atgatattgt
    22981 ctattatcca gattgcgatg atatgagtga tgtggcgcat tacttgattg tagaagctgg
    23041 tgattttggc gaagttccag aacaattgaa gccgtatatt gattttgatg cttgcggtcg
    23101 agatttggag attggaggaa attatttggt tactagtcgt ggagtttttg agtatccaga
    23161 atgaatcaaa cactatatat agtgtttgat tagtatatgt aagcaaaaaa acacaatata
    23221 tagagtgcaa actgaagggt aatgtgatat aatatttgcc gaagaaatat ttttgaaatg
    23281 aggtaatatt atggcaaata ttcaaacaag ttttattgat ttccataatt caattagatt
    23341 ggacgtagaa gacaatacgc tacttaagga ctataaagac caagtaattg atggattaaa
    23401 agattactta cctgatgatg tgaagtttga aactttttta cagggaagct attctgttta
    23461 taccggaata aaaagctgtg atgaaaaaat tgattttgac atcgatattg cagttgcttt
    23521 cgaaattgat catactgttt atgaagatcc aagggagcct aaactgtggg taaaagaggc
    23581 gctagttgaa atttttccta atgctcaagt taatttaaaa gtaccttgtg taactgctac
    23641 atttactgga aaaaagacaa aaaaaaatgt gcacgttgat gttgcggttt atgctaaaga
    23701 agatgaaaat tatttcttag caaaagcaaa agaattcagt gctcccgaaa accgatgctg
    23761 ggaggaagcg gatcccaaag tattaaaaga gaaaattaat agtcatgtag cagattcaga
    23821 tgatcgaaaa caatttcgaa gatgtattcg ctatttaaaa agatggaaag ataacaattt
    23881 taaccaagaa tataaaccaa caggaattgg acttacaatt aatgtgatgg atacgttcct
    23941 agttaataag tccactgatt ttttaactag aaaagttcag tataatgata tggaatgtat
    24001 gaagcagatt gtttcttcgc tgaaagattc atttgtgtat gagtacagtg aaacagatgg
    24061 ttggcattat cgtctacatg catatcttcc agtgaagcct aacagtgaca cctattcaaa
    24121 aatgacagtg aatcagatga gtgattttaa aaacaaactt tctaaattat atgatgattt
    24181 gattttcgct atcgatactg aagatgagta tgaagctaca aaacgattaa ataatcaatt
    24241 tggggaggat tttttaatta tttcagaaga agaagttaca gaaaaaaact taagaaatgc
    24301 atttgttaca gactatccga gtgcttaggt tggtgttaaa gattggatta tactgatatt
    24361 ttaaaagaaa agctaacaga tgttgtgcgt ttattcgatg gtaattacta tggggatttt
    24421 tcatttgcta caattggaaa gttgcgttta aaaattgaaa taccaaaaca ttttccattg
    24481 gtacttccta ggatttacat tcataatttt gatcatttag gactttacct accacatgta
    24541 gaaaaagatg ggttggtatg tttttcttca gatataaatt atttagtaga tgaagaaaat
    24601 tcagaattgg ttttattgca gtgtgttaat aaaactataa aaacaattga ggaaggtttg
    24661 aaagggttaa ataaaaatga ttttcgaaaa gagtttgttg ctttttggga caaacaagag
    24721 aatcaaattt cgttattcta cctttgtgaa ccagcgttgt ttatcaagtc agttgataca
    24781 ataatgtacg gtaagtcttt ggttgctttg gataaagaga acgaattaca attaaatgca
    24841 gcgagtagat tgtttaatgg agagaaagaa gatactcatg aattagatgc atactatgtt
    24901 ccaattagaa acaagaataa tattatgccc ccgaagaata ataaagcatg gtcaaagaag
    24961 gatattttac aaatagtaaa aaaaaatgta acaggtgcaa ataaaagatt ttttttaaaa
    25021 cttgaaaaaa gaaagaatag acataatgtt ttacttattc tagatatccc aattgaagaa
    25081 aagtcaaata atgtcataat tggatttctt tttacaaaac aatctggatc tataaaagat
    25141 tataagggta cgcctaggac tgtattagtt cagcgtcatg atgaattata tttggtgaat
    25201 agaacgagcg gagaaaacta tggattgggt gctaatatag ctattgttgg ggtagggtca
    25261 gttggcagta aaattgctga aaatcttggt ccattaggtg tgagaaagat gacactcata
    25321 gacaatgatt gttttcaagc tgaaaatttg tatcgaaatt cacttggtgc agatagttta
    25381 aaaaatgccc gatctcagaa taattttttt aaagttgatt taatgcagga ggagctcgaa
    25441 aaaaaattcc catacctttg tgttgaagtg gagtcaatga acgttttaga cttaattagt
    25501 atgcaacctg gttttttcaa ggaatatgac tatgtttttg tttgcatagg agatacaatg
    25561 gcaagcctgg ctttaaataa ttatttttcc aaaagtagtc aaaaagtttt ctattcttgg
    25621 ctggagccat atggcattgg gggacacact ctatttataa attattctct aaatcaaaaa
    25681 ggttgtttca attgtcttta tacagattca aactctaaaa ataggtgttc aaatagagct
    25741 tctctggtaa aagaaggaca aatttttgaa aggactttag cgagttgccg ctcaaaattt
    25801 ataccatata atgctattgc ctcgacggaa tgtgcaatta atgcaataaa acttttccat
    25861 aaagtcaatc aaaataatat gaccaatagt actctttttt catggatagg agatgggagt
    25921 acttttgaga atttaggata tcaatatagc gacagaaata cttttcttaa tggagtcaat
    25981 aagtttgaag aaagattctt ttctaaatat tgcaaagtat gtggggagtt agatgattaa
    26041 attttcgtgt gaccgtattg tcattgaagt aagggatgaa gttcttaaga tatttgaaca
    26101 atatcggcag ggaaaggatg acttgcctga agctggagga gttcttcatg gaaaaagact
    26161 taagaatggg agcataataa ttaccgatat aacaacacct caaataggag atatacaaaa
    26221 aagattactc tttaaaaaaa accgagaaat tcatcaacag ctttctaatg agaaatggat
    26281 agaatcaaat aagttttcta ttgcattggg agaatggcat actcatcctg aagctgtacc
    26341 aactccatca aatgttgata agaaaagctg gagactgaac gtgagcaagc aacatgatga
    26401 tagagtttat gtttttatta tcgttggatt ttctgatttg agaatgtggt tactgtcaaa
    26461 aaaatcagat attaggcagg ctataaaact ataaaggagt gattaggtga aatcacaaaa
    26521 gaatatttgg tggtgggggt ttttagtagg aaaggtaatg ataccagccc ttctcttaat
    26581 ttctctaccg atgattactt ttttctttac caataaaaaa atgagtaaag aagccgtgat
    26641 ttttttcttt ggagaccaaa aagctacctt tattgaaacg ttagtaacat ctcttcagct
    26701 taatttgaat tacatttttt caattattat tgttatatcg ttattgaatt tttttaaaaa
    26761 aagaaattca ggaaaggtgt ttaactctaa tggaaatgtg tactacaact atttttattt
    26821 tgtattttgg gtagcagcaa ctttacttgg ttatgataaa attcagattg caggaattcc
    26881 tatacacatg caatataagt tggttttgag tggaattttt tctgaggtgt tgccagatat
    26941 atacgatgat cattacgaca gtgatggaac atgtaaagta agcattgaaa aagaaaattt
    27001 cgatgacatc gatggatatg actctgtgaa cttactgatt attgatactt acgatataaa
    27061 aatgagtgaa ttatctatgg aaaatcaaac atatcctacg ataattgtta gagggaattc
    27121 aatagatgga gttcgtaaag ttaatagatc tttaatcttg gaaataaaaa aaacaatgga
    27181 tgaaattcaa aaaagtgact ttaaaaaagt atttgttgct tcaacatcta atccaaaaaa
    27241 tagtatcaat attattaatt catcgtttag atttttcggt cgatcacgta ggtttaaact
    27301 ttatgttctt caaaaggatt atgcaagcaa tggaaagtat agtaagaaat atagaatttt
    27361 tatttaagaa ggaatttttc cttcttttta ttttggagga aactatgaaa aaaataaaaa
    27421 gctacaccag tatttgggca gttgaaaaag tcatttatgc gattaatgac tttcagctgc
    27481 cttttcctgt gacctttaac cagatggctt ggtttgtttt atccttgatg tttgtcattg
    27541 tatttgccca tgtgcctccg ctatccatga tagagggggc ttttttaaaa tacttgggca
    27601 ttccgatagc ggtcacttgg ttcatgtcgc aaaagacctt tgacggtaag aaaccattgg
    27661 gatttttacg atcctttatc agctatcact tacggtttaa ggtcactttt gcaggaaaga
    27721 aagttaaaga acagaagaaa cgatgggacg aaccaatcac cttggtaagg agtgtgaatt
    27781 atgtacccga ttaagtatat tgaaaataat cttgtcttta accaagaagg cgagtgtttt
    27841 gcttactatg aattagtgcc ttacaattac tcctttttgt cacctgaaca gaagtatcaa
    27901 gtacatgata actttcgcca attaattgcg caaaatcgtg aaggtaagat ccatgcctta
    27961 caaattgcca cagaaagtag tattcgtgcc acacaagaac gttcgaaaaa ggaaattacc
    28021 ggtagactca aagaagtagc aaagcaacga attgatttac agacagatgc attagtatcc
    28081 atgattggcg atagtcagat tgattatcgc ttctttattg gctttaaact gatttcgaca
    28141 gatgaagagg tcaatctaaa gaatctcagg aaatctttct tttctggact tcaagaattt
    28201 gtctatggag tgaatcatca tttgatgggt gattttgttt ccttatccaa tgaagaaatt
    28261 cgtcgctata ccaagcttga aaaactaatg gaaagtaaat tggctcgaag atttaaagtc
    28321 aaacgagtaa cgccgagtga cttgacgtat ttaattgaac atatctatgg tgaaaaaggc
    28381 acaccgtttg aagaatatga attccagcta ccaaagaaaa agctgaaatc agaaacgctg
    28441 gtgaaacgtt acgacttatt acgtcccact cgttgtttga ttgaagaaaa gcctcggtac
    28501 ttacggatgg aacatgaaaa tcatgagtct tatgtagcgt acttaacaat taataccatt
    28561 gtaggggaaa tggagtttcc ttcctcggaa cttttctatt accagcaaca acaatttacg
    28621 tttccgattg atacttcaat gaatgtggaa attgtgacga ataaaaaggc cctagctacc
    28681 gttcgtaata agaaaaagga attaaaagat ttagacaacc atgcctatca atctgataat
    28741 gaaacgaact ccaatgtatt agatgctttg gattccgtgg atgaattgga aaccaccttg
    28801 gatcagtcga aagaatccat gtataaacta agctatgtgg ttcgagtgag tgctaaatca
    28861 gtggatgaat taaaacgccg ctgtgatgaa gtgctcgatt tttatgacga taccaatgta
    28921 aaactagtcc ggccctttgg cgatatgatg ggtctacaca aagaattctt accctcaagt
    28981 aagcgataca tgaatgacta tattcaatat gttacgtctg actttttggc gggacttggt
    29041 tttggggcta ctcaaatgct aggagaatta gaaggcattt actttggcta taacgtagat
    29101 acggggcgca atgtttacct gaaaccggca ttggcatcac aaggggtcaa aggctcagta
    29161 accaatgctt tggcatctgc tttccttggt tcgttaggtg gggggaaatc ttttagcaac
    29221 aatctcttag tctattatgc cgtccttttt ggtgggcaag ctgtgattgt cgatccaaaa
    29281 ggagaaagag gcggttggaa agaaacgttg ccagaaattg cggatgaaat taatattctt
    29341 aacttgacca gtaagccaga aaatcaaggc ttacttgatc cctatgtgat tatgaagaaa
    29401 aagaaagact cagaaagttt ggcgattgat atcctgacct ttttaacagg gatttccagt
    29461 cgggatgggg agaagttccc tgtgttacgc aaagccattc gaacggttac ccaaagtgat
    29521 cagcgaggat tattacaagt aatcgttgaa ttgcgtaaag aggactcacc agtcgcggaa
    29581 aatatcgcgg atcacatcga gtcctttacc gattatgatt ttgcccattt gcttttctca
    29641 gatggttcga ttacccagtc gattagttta gataagcagt taaatatcat tcaagtagcg
    29701 gatttggttt taccagatgc agaaaccact tttgaagaat ataccaccat ggaattgctg
    29761 agtgtttcca tgctaattgt tatttccact tttgccttgg actttatcca tagcaaccga
    29821 gaaattttca agattgtgga cctggatgag gcttggagtt ttcttcaagt agctcaagga
    29881 aaaacgttat cgaataaatt agtgcgtgct ggtcgggcca tgaatgccgg tgtttacttc
    29941 gtcacgcaaa acagtgatga cttgttggat gaacgtttga agaataatat tgggctaaag
    30001 tttgcatttc gcagtaccga tattcatgaa atcaagaaaa ccttggaatt ctttggatta
    30061 gataaagaag atgaaagtaa tcaaaaatgc atgcgagatt tggaaaatgg gcaatgcttg
    30121 atgcaggatt tgtatggtcg agtaggtgtg gtccaagttc atccagtctt tgaagaactt
    30181 ttccatgctt ttgatacccg tccgccagtt caagagagaa tggagtgagg agatgaaaaa
    30241 gaaacttttt cgtatctttg gaattgtttt aatcagtttg gtcactctga taatactttt
    30301 gagcttagtt ggaacggtag ctgaggcaac aggtctggtt gatgatacag ttggagccgg
    30361 aaatctttat tcccaatatt cacttggtaa ctatcagttg gatttctttg tggatagttc
    30421 gtgggattgg ctgccttgga attggggtga tggcctaggg aagagcgtaa tgtatggact
    30481 ttatgccatt actaatttta tttggacagt tagtctctac ttatcaaatg ccacgggcta
    30541 cgtggttcaa gaagcctata agctggattt catttcagat acggctgaaa gtattgggaa
    30601 gaatatccaa accttagccg gcattaccga aaatggactc cagacctcgg gcttttactt
    30661 tgggtttcta ttattgatga ttttagctct aggagtttat gtggcgtata caggattatt
    30721 aaaacgagaa actaccaaag cagtccgggc tgtcattaat tttgtgatga tttttctact
    30781 ttcgggttcg ttcattgcgt atgcgcccac ctatatcaca aaaatcaatg attttagctc
    30841 agatgtcagc gaagcggctc ttagccttgg aaccgagatt gtcgtgccga attcagaaag
    30901 ccaaggaaag gacagtgtgg atttaatccg agatagcttg ttttccattc aagtccaaca
    30961 accgtggtta ctcttgcaat ttgatgattc caatatagaa gaagttggcg aagatcgtgt
    31021 taacaaaatc ctgtctgtaa gtccagatga aaataaaggc aaggatcggg aagaagcggt
    31081 caaagcggag attgaagata atgacaatgc gaatctcagt attaccaaga ccatgaaccg
    31141 cttagggacc gtggtctttt tggtgttgtt caatattggc atatccttct ttgtctttct
    31201 cctaacaggg attatgttgt tctcgcaaat tctctttatt atctttgcga tgtttttgcc
    31261 gattagtttc ttattgtcca tgttgccgac ttatgagagt ttgggcaaga aggcgattat
    31321 tcgcttgttc aatacgatta tgatgcgagc aggagttact ttggtgatta cgactgcatt
    31381 tagcatctcc acgatgtttt tcaatatttc ggcaacctat ccatttttca tggttgcttt
    31441 tctgcaaatt gtgacctttg ccggtatcta tttcaaattg ggcgacatta tgaatatgtt
    31501 caatctccag agtaatgata gccaatcaat gggaagacgg gtcatgcgaa aaccgcaaat
    31561 gttaatgaat cgaacattgc ggcaactcaa tcgaaatgtg ggacggactt tggcttttgg
    31621 tggtggtgca gcagttggaa ataagttggc aaaggaacaa tcaaaatcca aatttaagcc
    31681 atccggttct tctctacgaa agaattcacg attaccgaac gatcacgaga taccatctga
    31741 cttgaataag gaaaagccaa ttccaaataa taagaaacaa tcccgaatga atttggccgg
    31801 gagaaaagtc ggcaaggttc tggataccca agctttggtg aaagataaga ccaaacaagt
    31861 aaaggatgga gtacggaata cgccaaccaa cttgaaatac aacttccata aaggaattga
    31921 aaaaacgaaa aaagcaccgg aagagtttaa gcgaggactg gtccaagaaa aagcaaatcg
    31981 aggagagttg cgagaaaaac aacaacaacg tagagacgaa aaaatagctg aaaagcgcaa
    32041 agtattgaac gaaataggga atcctcatgg gaaaagaagg gaaaatgttc cgataaaagc
    32101 aaaggtccaa tctcaaaagg atagagcacc aaagcggatt gtgagagcaa attcgaatcc
    32161 cgaaatcaaa cgaaaattcg ctagccaaga aatcatttca aaaggaataa accaatcctc
    32221 aagtaagaaa aattcttttc aacaagtgaa acggcggtct actctgtcca aacgaaccaa
    32281 tcgaaaagta caaaaaagtt tgaaccctca tcccaaacca aggtcaggtg agaaaaaatg
    32341 aacgtaaaga aattaagtgc catttcgtta actttcctaa caatctcttt tatgggtatc
    32401 ttgatgtgtg tgggcttatt gtttggtgaa gacagtgaag gaagtagtag ttcaggtgct
    32461 cctcctgggg ggagtagtgt ctcagaagag gttttgaaac accgtgcaat ggtggaaaaa
    32521 tatgccaaag aatcggatat aacagagtat gttccgattc ttttagcgat tattcaagtg
    32581 gaatctggtg gcacaatgga agatgtgatg caaagttctg agtcagccgg tttaccaccg
    32641 aattcattaa gtaccgaggc atccatcaaa caaggatgtc tctattttgc gtctttggtt
    32701 aagcgttcca aagaattagg ttgtgatcaa gatagtatca ttcaggccta taactttggc
    32761 ggtggttatt tggactatgt ggcaaaaaac gggaagaagc atagttttgc tttagctgaa
    32821 tctttttcaa aggaaaaatc ttgcggtcaa aaaatcgatt accctaatcc catcgcgaat
    32881 aaggaaaatg gtggatggcg ctataactat ggcaatatgt tttattgctt attggtgaag
    32941 caatacctaa ctacaacaca gtttgacgat aaaacagtcc aagggatttt tgacgaggcg
    33001 tttaagtatg aaggaacggc ttatgtattt ggtgggtcaa gacccgaaac aggatttgat
    33061 tgtagtggct tgactcaatg gtgttatgcc aaagttggtc tgaaacttcc tcgggtggct
    33121 caagatcagt atgatgccat gacccatatt gacctaaaag atgccaaacc aggggatttg
    33181 gttttctttc attcgactta tgatgccggt acttatgtga cccatgtcgg tatttatgca
    33241 ggggagaatc gcatgtttca tgcaggagat ccagtaggct ggacgaatct aacagaaagc
    33301 tattggcagc agcatcttat cggtgctgga cggtacaaat aagagaaaag aggaaaaaga
    33361 atgaagataa aaattgaacg gaatcaaaag gaaaagagcc taaaaaagaa aaaagaacga
    33421 gtcgtatccg tgggtaagca tcgaaaaatg gtgcttgccc tttggctttt attatcttgt
    33481 agtcttactt ttggcgttta taaaaatttc accgccattg atcaacatac aactcatgaa
    33541 aaagtggtta ttaaagagaa agtagtgaat acttctggta ttgaaaactt tacgaaggat
    33601 ttttcgaaag aatatttttc gtggaaaaat aacaaagaag caattgaaaa gagaatgaac
    33661 aacctcgaac agtaccttac agaagaaggt ttcgctttaa gtcaagatat ggttcgagca
    33721 gatattccca ctaattccga agttcagtca gtaaaagtct tagatgttga aaagagctcg
    33781 gaggaatttg tcgtttcatt tttagtggag caaaagatta cagagggaaa aaaggcacaa
    33841 tcgatttctt ctgcataccg agtgacgatc tttgaagatg aaaacaggaa ttatatagta
    33901 acgagtttgc caactatgat tgcaaaacct gatagggcaa aatacaagac aaaacaagta
    33961 gaaaatgatt cgaaaattga tgccaaaact acagaagaaa tcgctgagtt tttggaaacg
    34021 ttctttaaat tatatccatc agcttctgaa aaagaattgg agtattatgt tgaaaatgat
    34081 gcgatgcgac caatcaatat tgacttaaag ttttcgaagt tagaggatcc agtttaccta
    34141 caaaacggaa aaaaaattct agtgagtaca aagataaaat accttgataa tgtatcaaaa
    34201 accactagct actttcagta caaattaata ttacaaaaaa atggaaactg gaaaatcatt
    34261 gatgttaaat aataatgact caatctaaat ttatgagaag ttataagtag tttaacttat
    34321 ttcaggcaga agtcgcccat cctctatagg tgggtgatga atgccgttcg gtaactcgaa
    34381 aaccgacaat taagacatag tgccttttta ttttaatcca tgtcataatc agtctatata
    34441 agaaatggtt gatataccat ggaattagat acaaataatc attcattatt tcttctttgt
    34501 taccacctta ttttagtagt gaaacattgt aaacatctca ttgatgatgt aatgttagct
    34561 tatgcaaaaa actatctttg aaagaatatc agaatcacat cacattacct tagttgagtg
    34621 aaatcgtgat aaagactact ttcatattat gctcaaagca caacctaaaa ccgaattaac
    34681 aaaattcagc aatgcctaag ctcatgtctg ataaaatgag actttctaag ggtcaaaaat
    34741 ttcctttgaa tacgatcatt agcttagata aaagtaaaaa ccttagaact acggggatag
    34801 cttggttaat aagagatatc gctgttaata aagacataag cttgcaagta tgctctattc
    34861 ccaagaatct cccacttcaa gcactaagga tcgttaaatt cagctaagtg gcaagtagtt
    34921 cactagacga taattatctc catatttcaa tctcagcttt tccatttttc atagcaaatt
    34981 ccgttatttt tatagcatct aaaagattca gcttttccac tgatctttca ccacttttca
    35041 gcttttttat tgtgcttaaa ttgataccag taccttttga tattgaattg gaagtaaaat
    35101 cattaagggt tttttttatt aaattcatat ccgcaattcc agcgttcata agaatgaaga
    35161 ttctcctctc atagactact ttgaaaatgg tactacagtg gctgtttata tatttcagta
    35221 ggttactgta gtggtcattt tattataatt agaaaaggaa cctaaatcaa aaatgctaaa
    35281 gaaaattttc atagtattaa acctatcatg actaaagcca cggaattctt gataaaagag
    35341 tatacttgca agcctatgtc tttactaaca gcgatatctc ttattaacca atctatcccc
    35401 gttgttccta aggttcttga tgttttctaa ggcaatgctt gtatacatag cccttgatca
    35461 aaatattgat actaacgttg acgtttcgat catgatgtgt acggcaaata ggacaagtcc
    35521 attctcgaat tgtgaatgac ttcttaccat ctgtgtgtcc acattctgaa ccaatttgac
    35581 tagacggaaa tcatttatct atcttgatga tttttttgac agaagtatcg aaaatcttta
    35641 cataatctgg gtgttgcatt cttatgcaga tgagagcgtt agctccgtat tcataaccag
    35701 ttgcctctaa aacgtagtcc atcggtggat acctagcttg cggttgccag tggctttcgc
    35761 agtcttcttg taatcaaatc gagcatctaa ttgagtacta gtattttgaa gataatactt
    35821 gagtttttcg gttttcagaa gttcacttca tcttccatca tccatggaaa tcccactcac
    35881 ccatacgcag gcctccaaaa attcttcaac aatggttttt cagacatttt tctgaatacc
    35941 tccttgtttt ttgatacgcc agatagtcag gaaaaaggca acaaaacaca aaacaaagct
    36001 gcctaaaaag gtaatcttca ttccatataa aaagacatcc ggccgatctg cgatatagtt
    36061 agttactcgt tggcccatct tagcagacat cgcttgatat aagaccgtcg tagaaagcga
    36121 aatcccgatg accatcccca aatttcgggc aaaggaattc atggagcctg ccagtcccaa
    36181 attttcgata gtgacagaac tcataaccat cgtattattt ggggactgga acaaggcatt
    36241 gccggcaccc atgatggcag tagcgacaat gtacagccac aaaggagagt ccactgacaa
    36301 aaacatatac atgacttggg ttaccgacaa taaacccaag cctactaata caaaaatctc
    36361 cgggccaacc cggtcggtca ggtatccgct taaaggcgcc ccgacgacca tcatcaaggg
    36421 aaagaccatc atcaacagtc cggcatagct aggactcaac ccgcgagcat tttgcagata
    36481 aaaaggaatc actacattaa caaagaagtt ggaagaaaaa atcaacaagg ctgtaatcaa
    36541 gctcatggta aaaatcttgt tcttaaaaat accgaaagaa atcaatggag aggctgcttt
    36601 cttttcaact tttaaaaagc tgacaaaggc aatcactgaa attacgaaca gtacaagggg
    36661 gactggatgg aaaaagccac tttcttgccc caaaaagatt gttccaaaaa ggctgataat
    36721 cgtagccgca atggcagcaa agccccctcc atcgattgta tggccgctca tggtgatatc
    36781 ttttggtaag aatttttcag caatcaacat cgttacgatt cccaccggta cattgatcca
    36841 aaaaatgtac gcccaagaaa attgggacaa aatcaagccg ccaagacctg gtccagcaat
    36901 cgagcctaat gaaacaagcg caccgattgc tcccaatgcc cgaccacgtt catgtatggg
    36961 aaagacttcc gtgatgattc cggcatttgt agccatagtc atactagcac caatggcttg
    37021 taccacccgt gcgaacaatt aaatacctag cgaatgattg aacccacaca acagcgagcc
    37081 caagacaaag atcagcgtac caatccgaaa tactttgatt ttcccacagc tatcgccaat
    37141 tttaccaaac agcaacaaac aagaacagac aatcatcaga taaacggaca ctacccattc
    37201 cgcttgattc attggtatgc ccatatcatc tgagatggta ggtagtgcaa tattgacaat
    37261 actgccatcc aatgtggaca tgaaggtaaa catagatata gcaatcaaaa tccaccagcg
    37321 atttttttgg acttttgcat cttcttgata actatgcata ataggatttc ctttctaatg
    37381 atcgtgtagc taagaacaat ataacctatg tctactgccc aacaacttct cgcgactcta
    37441 gccataggaa gcttgagtaa tttcgatttg ttattctcat gtaaccatgt aagtttgaga
    37501 tgctttattt gaacttactc tatagtaact agtgataatt cgctttactt tcttctagca
    37561 atttctaaaa gtcgacttca ttgttcttca gattaatagt gtgagctatc tcgtgagacg
    37621 taagaaaatt tccattctac tttttcccaa ttttttcata tttatggtgt tgcacctatt
    37681 tgttttccaa tttgcttttc aaatacattg ctatgatatt caattccata ggattatact
    37741 aaatgtcaat agtatgaata atatattata gatgtttata tatcactgtt aattattatc
    37801 cacaataatt aatgattacc tcgatatttg tcaatcaaag gttgaaacaa tttattttca
    37861 gcatcttttc taacttgagc tgcactctta atagaatcat acgatcctaa atagtacctc
    37921 ttttttttaa agttatatac gcaatccatt tctctttaga acgataaact cccttaacac
    37981 cacttgtatt atttttcggt aattttgacg ttagacactc tattactgtc ccttcaacac
    38041 atgtccttgt ttcttgcctt tttaaacatc cacaactttg agtatgtcca aaaaataagt
    38101 tactataact tatctcacac atactgccac actcacattg acaaacccat tttgttttcc
    38161 atgaacagtt attgtccaaa ggatactttg cagtaagtaa tccgaatttt ttattttcaa
    38221 tgtgatgctt ttttgatttc aaacatccac agctttttac atcacctttt agaagtcgct
    38281 ttgccgatac taactttttt ctcccacaca gacattgaca ttcccaaaca acagttcctt
    38341 cacttcgcag tttagttggc ttgattgcaa ctaacttacc aaaagtttta tttgtcaaat
    38401 ttttatattt atgcctattt ttataatttc cacatccaca acttttggtt ctacctgata
    38461 gtaagtttga tcgatataca atcgtttcat tcccacagtc acataggtaa cgatattttt
    38521 tatatccatt atgagtaatc tcctcaacaa cagtaaggtg accatatctt tccttcactg
    38581 cacctaccct cccaagaaag aagtaccata taatttcaat taacttttaa taaatcagaa
    38641 aaaaatattt tcaatttttc ttagtctgat tttatgacta atttataata caagactgaa
    38701 cgatacgtac acgaaatcct caagatagat aaattgtttt catctagtaa aacctgctca
    38761 aaatatgagc ataaagacgg caataataca ctcgatattc gataatggac ttgttttatt
    38821 tgtcaaactc atcgtaatcg agatatcaat tccagtctca atatcctgat caaaggtcta
    38881 agaatccaac cattttcttg ttactttgtc agattgatta ccttttttag tctccgaata
    38941 ataaggtttt cggatcaact aacatgctct gagaaagact actatctttg caaatttcca
    39001 aatgaaggtg tgggccagta ctattcccag tacttcctac atagccaatt atttgtcctt
    39061 gcaaaacatt atctccagtt tttactaggt attgttgctg gtgagcatat aacgcggttg
    39121 atccatcttc gtgttgtatc accacgtaat ttccccaaga gtagtgaaac tctgctttaa
    39181 caaccttacc agctttaact gcatgaatcg gctcaccctg tgtagctgcc atatcaagtc
    39241 ctcggtgaaa ttcatttcca cgatttccaa acaaactggt tattgaataa ttatgaatag
    39301 gcacttggta tccagtctta ctcatttgtg gacctttaac ttctttatca tagttggtta
    39361 aatcataggt tttaattaat tcatttagct tcaaatagta ttgtgtatct gttgcatacc
    39421 gtcctgtaag gaatttagtt gcttctcgat atgttttact attagctttt aatgctcctg
    39481 cataaaaatc tttatttcca ctaattccct tagttaataa tttggcataa tcttcaaaac
    39541 tttcctcata gttttcatac actctaaatt ttgattgggt cgtataaagc gttccattac
    39601 ccaaatcttc gtttgttacc ataatgacaa agtttccatt ataagttcct tttatcccaa
    39661 ataaattgta attcggtgcc tgagctaact ggctttgacc actggcagat tctaagatcg
    39721 cttgtgcaat cataacagaa gcatataaat catatttctg accaattttt ctcgcagact
    39781 caccaatttt cataataaaa ttttctacag attcattttt gtcaaaatga atttcttcat
    39841 taccagatag tattggttgt gattggaaag attctgttga ttctatttgc tgtggtgtcg
    39901 aaaaattttt tgtttgtcct tccgtttttg gcgcttcctt ttttgaagac tctatatggt
    39961 ttggtcttgt cactttcgaa gaagttattt cacttgtact acttgaagtt gttgaagatt
    40021 cggcacttga aatcgtacta ctttcacttg acgaagttgt tgaagactcg gcatttgaag
    40081 tcgtactact ttcacttgac gaagttgttg aagactcggc atttgaagtc gtactacttt
    40141 cacttgatga agttgttgaa gactcagcat ttgaagtcgt actactttca cttgataaag
    40201 ttgtcaaaag ttcactactc tctgctgtta catcggaatt actcatttca gtcgtctcgg
    40261 ttctttcttt tgcaattttg tatgttgaat tttcgcttgc caacacatgg aaaggtgttg
    40321 ctataaagag ttgggacatt aatataactg ctgatgcaag ttttaaagat tttttcattt
    40381 gatttacctc tcttttttct ctttaatagt gttttctatt aaaaattatg ataaatattt
    40441 tcaatgaatt gagtaataca gtatatttct tgatttcatt tagtaacgat tatgtttcaa
    40501 tgtttaatcc tgttactgcc tttaaaataa aattataggg ttggctaaaa ctaggatgaa
    40561 ataaataatc taacattttc aatttgtcta atgtaatttg ttcttctata gcaagagaaa
    40621 acattgataa tagtccagat aaatcttctt gactacaaag ttgtacacct actattctct
    40681 ttgtattttt ttcaaagcta attcttaatt ttacacgact attttttctc aaaaaaacag
    40741 gtctaatgaa atcttctatt tctatataat ctatagactc acttaataat cgtgctgttt
    40801 ttaaattcaa acctgctgcg taaaaattgt aattaaatat agaaaagcca tttgtaaatt
    40861 gggaaccaga aggtgtaaaa tcttgattgt caatatgttg agttgcaata taagctgttc
    40921 ttagagcatt agaaatagaa aaatcaacac tacttctatt aatggacgta aaaaaagttg
    40981 tagcacaatc cccaatagca tatatatctt ttatatttgt ttgctggtat tcattagtta
    41041 gatatgctcc actaacatct ctcaagagtt cctttttgcc aattggacta ttagatagaa
    41101 atcctatagc ttgtacgacc atatccatag agtattgggt accacttgta gtgactattt
    41161 tttcaatctt ttttcttcct aaatattttt taggactttc tgatagacaa agatgtattc
    41221 cattttttga tagtatttct tcaactttat ctgaaaaact acggtcgtaa taagtcgaca
    41281 acactctatc tgcaacatcg aaaagatata cttctttccc tctttttctt atggcttcag
    41341 caatttcaac tccaatatat cctgctccaa taattcctac ttttttaata gtattatcat
    41401 ctaaagcttt aaaaatctct ttagcatttg gaaaattctt tatcgaaaaa atattagcta
    41461 aattattccc cggtacatct aacataattt gctttgaacc tgtagctagt ataagtttgt
    41521 catattcttg ggaaaatctt gtgccatctt tttttacaca aataatcttt ttattttgaa
    41581 tatcaatttt gtcaactgct gtctccaaaa acaacttttt tatcttagaa tttagttctc
    41641 ttctattttg ataaaaaaga tcttctaaat tgctaacttc tctttttatg aacaacgatg
    41701 tcccacattc taagtaacta atttctgatt ttttatctat cagaataact tcataatttg
    41761 aatcacataa gtttagaaat cgagctgcag ctagccccga atggtttgat ccaataatta
    41821 ctattttttt cacttaatca cattcttttc ccttctatga aaagtatctt catttttaac
    41881 taacaaccta tctcccaaaa taaaaaacaa aaagaggaag aaaaagattc ttcctctttt
    41941 cagagaagat ttaagaaatt gattttctat tcattctctt tttttcgatt tttataccct
    42001 aataacccta aagttcctaa caggattaaa ccaccactaa tagtaaagat attactatca
    42061 atctctcccg tcttaggata agcttttgag gactgctttt gaggactgct tttgcctcta
    42121 tttttgtttt gcttgctacc ttgattgtca gtcttattat ctttactaac ggctttttca
    42181 ctgtcttttt cgtttgaaga aacaactaca ttagtaggtt cactagtatt accagattca
    42241 tcagttgcag ttactatcac ttttgttcct gcctctaatt taggaacttt aactgtaaaa
    42301 ttaccatttt tatcagctgt cccatggtat tgactcccgt ttggtaatgt aactgtcacc
    42361 tctgcatttg gttcagtttt accagtaaca atttggtcac cctctgtaat aggatcaact
    42421 tgaggtttat tcggtgcttt tttatcttct acagaaatag taacctctac ttcttcttta
    42481 gatccatctg gatatgttac tactacaatc ccttttttgt ttccagctgt actaacatct
    42541 actttctctt tccaagtata agtagttcct tttggtagat cttctttgtt cttaattccc
    42601 tcagatgcgt ctggctcttt atttaagtct gtagtcactt tttgtccttc aggatt
//
LOCUS       DACCSK010000009        41500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCSK010000009 DACCSK010000000
VERSION     DACCSK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.009"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IVP39_000441"
     CDS             complement(1273..1938)
                     /locus_tag="IVP39_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7585541.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IVP39_000442"
     CDS             2064..4100
                     /locus_tag="IVP39_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7585542.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IVP39_000443"
     CDS             4104..4742
                     /locus_tag="IVP39_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7585543.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IVP39_000444"
     CDS             4770..5198
                     /locus_tag="IVP39_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585544.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IVP39_000445"
     CDS             5655..6566
                     /locus_tag="IVP39_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7585545.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IVP39_000446"
     CDS             6783..7220
                     /locus_tag="IVP39_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585546.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IVP39_000447"
     CDS             7315..8199
                     /locus_tag="IVP39_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7585547.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IVP39_000448"
     CDS             8423..11590
                     /locus_tag="IVP39_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585548.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IVP39_000449"
     CDS             11610..12155
                     /locus_tag="IVP39_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP7585549.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IVP39_000450"
     CDS             12183..12638
                     /locus_tag="IVP39_000450"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP7585550.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IVP39_000451"
     CDS             12635..13393
                     /locus_tag="IVP39_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7585551.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            13894..15093
                     /locus_tag="IVP39_000452"
     CDS             13894..15093
                     /locus_tag="IVP39_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7585552.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            15115..15327
                     /locus_tag="IVP39_000453"
     CDS             15115..15327
                     /locus_tag="IVP39_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7585553.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            15402..15878
                     /locus_tag="IVP39_000454"
     CDS             15402..15878
                     /locus_tag="IVP39_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7585554.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            15875..17683
                     /locus_tag="IVP39_000455"
     CDS             15875..17683
                     /locus_tag="IVP39_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7585555.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            17760..17975
                     /locus_tag="IVP39_000456"
     CDS             17760..17975
                     /locus_tag="IVP39_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7585556.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            18067..18930
                     /locus_tag="IVP39_000457"
     CDS             18067..18930
                     /locus_tag="IVP39_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585557.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            18961..19503
                     /locus_tag="IVP39_000458"
     CDS             18961..19503
                     /locus_tag="IVP39_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7585558.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            19517..19939
                     /locus_tag="IVP39_000459"
     CDS             19517..19939
                     /locus_tag="IVP39_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7585559.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            19869..22268
                     /locus_tag="IVP39_000460"
     CDS             19869..22268
                     /locus_tag="IVP39_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7585560.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            22300..24291
                     /locus_tag="IVP39_000461"
     CDS             22300..24291
                     /locus_tag="IVP39_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7585561.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            24314..24565
                     /locus_tag="IVP39_000462"
     CDS             24314..24565
                     /locus_tag="IVP39_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7585562.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            24555..25784
                     /locus_tag="IVP39_000463"
     CDS             24555..25784
                     /locus_tag="IVP39_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7585563.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            25781..27862
                     /gene="topB"
                     /locus_tag="IVP39_000464"
     CDS             25781..27862
                     /gene="topB"
                     /locus_tag="IVP39_000464"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7585564.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            28011..31931
                     /locus_tag="IVP39_000465"
     CDS             28011..31931
                     /locus_tag="IVP39_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7585565.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            31932..32876
                     /locus_tag="IVP39_000466"
     CDS             31932..32876
                     /locus_tag="IVP39_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585566.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            32880..33083
                     /locus_tag="IVP39_000467"
     CDS             32880..33083
                     /locus_tag="IVP39_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585567.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(33383..33619)
                     /locus_tag="IVP39_000468"
     CDS             complement(33383..33619)
                     /locus_tag="IVP39_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7585568.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(33802..34173)
                     /locus_tag="IVP39_000469"
     CDS             complement(33802..34173)
                     /locus_tag="IVP39_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585569.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(34170..35498)
                     /locus_tag="IVP39_000470"
     CDS             complement(34170..35498)
                     /locus_tag="IVP39_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7585570.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(35459..35788)
                     /locus_tag="IVP39_000471"
     CDS             complement(35459..35788)
                     /locus_tag="IVP39_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7585571.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(36046..36417)
                     /locus_tag="IVP39_000472"
     CDS             complement(36046..36417)
                     /locus_tag="IVP39_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7585572.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            36562..36747
                     /locus_tag="IVP39_000473"
     CDS             36562..36747
                     /locus_tag="IVP39_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7585573.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            37259..37477
                     /locus_tag="IVP39_000474"
     CDS             37259..37477
                     /locus_tag="IVP39_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585574.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            37690..38349
                     /locus_tag="IVP39_000475"
     CDS             37690..38349
                     /locus_tag="IVP39_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7585575.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            38352..39695
                     /gene="vanS"
                     /locus_tag="IVP39_000476"
     CDS             38352..39695
                     /gene="vanS"
                     /locus_tag="IVP39_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7585576.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            39866..40673
                     /locus_tag="IVP39_000477"
                     /pseudo
     CDS             39866..40673
                     /locus_tag="IVP39_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
     gene            40691..41500
                     /gene="vanW"
                     /locus_tag="IVP39_000478"
                     /pseudo
     CDS             40691..41500
                     /gene="vanW"
                     /locus_tag="IVP39_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT        11617 a         9541 c        10341 g        10001 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaat acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaatttt aggttatcgc tgaaagcccc ggaaatacgg
    13681 gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg gattgagccg
    13741 catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca tcataaattg
    13801 aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag gacgccgctt
    13861 ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg ccctgaccgg
    13921 gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg cggcggggcg
    13981 gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa atatcaattc
    14041 tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc agcggtcaaa
    14101 ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc
    14161 tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa ggccaagggc
    14221 ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa aggactgtcc
    14281 tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct tgaaaaagct
    14341 ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga tatggtctat
    14401 accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac atcggataac
    14461 ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac gcaattaaat
    14521 aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga tggacaaagt
    14581 acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga ggcagcggct
    14641 acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta tcgggaaatc
    14701 attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga ccgtgaacag
    14761 cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa gacaatccgt
    14821 gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa gctgaccggc
    14881 gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt ccgcaatatc
    14941 aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa ctactacacg
    15001 gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc cggcctgccg
    15061 gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga ccctatgaag
    15121 cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt tgatctggcc
    15181 gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg
    15241 aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg ggccgacgac
    15301 ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg gtggttccgc
    15361 cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa gaagttactc
    15421 aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg
    15481 cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac cacggccagc
    15541 agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag atcaccgagg
    15601 ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt accttacgaa
    15661 aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag gatgcagata
    15721 accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag cagcggccct
    15781 ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg
    15841 ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa agaaattgct
    15901 gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta
    15961 ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg atggttttgc
    16021 ggcggctttt gccaacccgc tgccaagcct gcacctgttt gacctgtgta tcggtatcgc
    16081 cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga agaaataccg
    16141 caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag cgccttacat
    16201 cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta ccatgaccgg
    16261 caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg
    16321 ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc cgaccacgga
    16381 ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag
    16441 gatgttccag caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc
    16501 catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa
    16561 taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa
    16621 gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga
    16681 ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga
    16741 cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga
    16801 ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc
    16861 catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct
    16921 gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct
    16981 cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct
    17041 aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt
    17101 acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat
    17161 tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagtt
    17221 aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt
    17281 ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat
    17341 tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca
    17401 aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg
    17461 tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca
    17521 tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt
    17581 tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct
    17641 gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat
    17701 tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta
    17761 tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg
    17821 ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg
    17881 caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca
    17941 ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac
    18001 ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg
    18061 ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat ggacggtatc
    18121 acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc
    18181 gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct gcacagcctg
    18241 tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat gtgctatgag
    18301 cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc catgttcttc
    18361 cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc
    18421 atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg
    18481 cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt ggaggctatg
    18541 agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct caccatgaat
    18601 atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat ctatgttgtc
    18661 acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg cggcatgggc
    18721 caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat catggttgtg
    18781 gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat atccggcgca
    18841 atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt
    18901 atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca caggaggaac
    18961 ttgtttattt caacgtacaa catcatttac gctgacccgc cgtggcggta tgactgcaaa
    19021 aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga gctgtgcgcc
    19081 ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc cacattcccc
    19141 atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa gaccgtggct
    19201 tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct gggccgctgg
    19261 acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct
    19321 gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa gccggatgtg
    19381 acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct gtttgccaga
    19441 cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg
    19501 tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc atgtgaaaag
    19561 caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg cgctggtgat
    19621 cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg cggccctgct
    19681 catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc acggccagcc
    19741 ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa aggagcgtcc
    19801 ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg aacaggaggt
    19861 aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg
    19921 cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc aggccccaca
    19981 cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc
    20041 ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt gcccagccgg
    20101 acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg gacgcctcca
    20161 ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac gccaagagct
    20221 ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac accggcattc
    20281 tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac ctgacattca
    20341 ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc ggctttgacc
    20401 tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc tgggaacggc
    20461 tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt gactggaagt
    20521 ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc ctgtgcttcg
    20581 gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc
    20641 tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa agcggtgttt
    20701 tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc atcaagcgca
    20761 aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc cgcagcggtt
    20821 acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc aagaagctgc
    20881 tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt gtcctcaacg
    20941 tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc gtggcccaaa
    21001 agcataactg tccccttgtc cggctggact accagcagga acagggcctt gcaagcagcc
    21061 tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc agcgtggccg
    21121 tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg tattacggca
    21181 tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt tgccccaatg
    21241 ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc gagatcgtca
    21301 gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc gaatattacc
    21361 cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc aaggactttg
    21421 tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg gccctgaaat
    21481 ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg ctggaagcca
    21541 ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat ctggcagacc
    21601 cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta gatcagcatg
    21661 tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg
    21721 tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt gacattaagg
    21781 agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag atatgggggc
    21841 gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac gagttccatt
    21901 tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag cggtttagga
    21961 aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt tcctccccgg
    22021 aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag gcgtccgggg
    22081 atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag tacatcgaca
    22141 attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc
    22201 ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg
    22261 gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct gaaagccaag
    22321 gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca ggccaccggc
    22381 gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc
    22441 ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca taaggccaag
    22501 aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct gcaccggcca
    22561 tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca gccctatatc
    22621 cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat ccccaagaag
    22681 cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg
    22741 cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc aggccgtcct
    22801 gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca tgaaaacgtg
    22861 ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg aaaagccatc
    22921 cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa ggccgagcgc
    22981 aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga taacccggag
    23041 atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca gatcaagcgc
    23101 cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc acagggcgcg
    23161 gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg
    23221 gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt gttggggctt
    23281 ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg cagcgccgga
    23341 agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc cgccgaggac
    23401 gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga ggccacccat
    23461 gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta tgtgctgatt
    23521 tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca gggtgatttg
    23581 tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga aacccgttac
    23641 cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga cacctaccaa
    23701 gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct gtcccatgtg
    23761 ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat gtccacgcta
    23821 ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac cctgacgccg
    23881 ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc cgccatgctc
    23941 aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc tcccagcacc
    24001 agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg gaacgtggga
    24061 cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc gcaggccaag
    24121 cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc
    24181 ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta cacgaacctt
    24241 acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta acgccagaaa
    24301 ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa
    24361 gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga aaaccgaaca
    24421 ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg aggaatttgc
    24481 cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt accatgaaca
    24541 ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc cccgcacttc
    24601 tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc
    24661 cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt ttggagcctg
    24721 aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa aaagataccg
    24781 gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt tccaaggtga
    24841 gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa aaagaacctg
    24901 aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc
    24961 ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta gtcggcacag
    25021 acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat tccgactata
    25081 tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc ctctccattg
    25141 acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc agcgccgccc
    25201 tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg aaaaatggca
    25261 agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt attgaccggg
    25321 acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc gatctgctga
    25381 cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt accacgaaat
    25441 gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg accgagtgca
    25501 gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag gaagaaccca
    25561 agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg
    25621 ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc gacgatttgg
    25681 gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa accgagtttg
    25741 acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata aacttgtaat
    25801 cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca cggagaaaca
    25861 cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac atctggtgtc
    25921 ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc
    25981 cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc agtttgacgt
    26041 tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg
    26101 gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt
    26161 tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct ttgcccacct
    26221 gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg ccaaggccga
    26281 ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca agaacctgac
    26341 tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg ccaagattac
    26401 cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg cggacgcggt
    26461 aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc
    26521 gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc ccaagctcta
    26581 tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg ccaagcagac
    26641 ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga gaacggatag
    26701 tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga
    26761 gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca tcctgaacag
    26821 caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga agcagggctt
    26881 tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca agctcttatg
    26941 tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct gtgccgggca
    27001 ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga ttgaacgccg
    27061 gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc
    27121 ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg agcatttcac
    27181 cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac gggccggggc
    27241 cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca cccgcgccgc
    27301 cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc agctcattcc
    27361 caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt ctccccagct
    27421 tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg atcctgacga
    27481 atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt cctgtatcag
    27541 tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt gtccccgctg
    27601 tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt
    27661 cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca ccccgaagat
    27721 cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac
    27781 cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt acgtcaacta
    27841 ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag tgtggctaca
    27901 ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc ttgttgggga
    27961 agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag
    28021 tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc
    28081 tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt ggcattactg
    28141 ccgacaatcc gcaggatttt tttatcaacg ggtattcctg cccggaggac agacaccttg
    28201 ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc cttgccgccc
    28261 ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg caaaatcccc
    28321 gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg gattactatg
    28381 tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg aaccgttccg
    28441 gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag tttggacgcc
    28501 acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg agaagcggcg
    28561 acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg gtgatgcact
    28621 acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag gcatccgaaa
    28681 gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg aacagcaaag
    28741 aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt tttgaaagta
    28801 gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt ctgaacaata
    28861 cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg tggcaggatg
    28921 aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc ccggccccgt
    28981 tcaaggttaa gcagcagatg gagaaaatcg acccggccac ccagcagccc gtgatcggcg
    29041 cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt aaggtggtga
    29101 cagtctttga catcagccag accgagggca aggattttcc catgccctat atcagtgaac
    29161 ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg acatcaccct
    29221 atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc tacgcaaaac
    29281 agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc gccatccatg
    29341 agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag gaacaggcgg
    29401 cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat accgtctgcc
    29461 agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat tggagcagcg
    29521 gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg gccagcgaat
    29581 tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag gcccggcagg
    29641 aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg caggccgagg
    29701 aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg ctatggagcg
    29761 aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg
    29821 cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg ttccgcattg
    29881 actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt ggagataggg
    29941 acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag gacgaaaact
    30001 ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac aaggcccatc
    30061 gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc cttgcccaaa
    30121 tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg gaggaaacgg
    30181 cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc aatcagggcc
    30241 agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg caggactaca
    30301 aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg accgtggagg
    30361 aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc taccagcttc
    30421 gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta caggccgcag
    30481 ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg gataaaagca
    30541 cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat tttaggggac
    30601 acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac accgcccact
    30661 atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat cccctgcgta
    30721 ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc aacaatacgc
    30781 cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg gcagttgctt
    30841 ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct gtccgctatt
    30901 atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt tctgactacc
    30961 ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg gaaattgcgg
    31021 agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc ctccttgaca
    31081 cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc gcccgcgtcc
    31141 cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg
    31201 ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt gacaaaatcc
    31261 gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc aagctggaaa
    31321 agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc
    31381 gcaagcatgg gacgctggac ggatgccccc atctgaccga aagaggcatt gaaaacctga
    31441 aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc tggcagctct
    31501 ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg acccgccaaa
    31561 aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc aaccgggtgg
    31621 ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc gacgagctga
    31681 aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca
    31741 ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc gaaaagccca
    31801 gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg gcgcagctca
    31861 acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca aagggattgg
    31921 aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg gaacagatca
    31981 tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac attatcaggc
    32041 gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc cggccctccg
    32101 ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag tacaacacat
    32161 ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg gagctgaccg
    32221 gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg cagcatttcc
    32281 ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat gagcatggca
    32341 cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta ttgggaaact
    32401 ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg ctacggacgg
    32461 agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc accgctttgc
    32521 atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa gatctgtccg
    32581 cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac tttgcggcga
    32641 ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa tccctgtgtt
    32701 ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac gagcgccggg
    32761 atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac agcaaacagg
    32821 ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag gtataaggaa
    32881 tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat gcagaccggc
    32941 gcagaacgat aaacaggctc accgccgtca tgccggttat ggatggggat atgcgggcgc
    33001 aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat gacggccaga
    33061 agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc catgtggtcg
    33121 cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc ggtattgtcg
    33181 gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct tgcagcgggc
    33241 ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga aacagatata
    33301 gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa
    33361 gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc aatgcttcca
    33421 gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc tttttgccgt
    33481 ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa tgggccaaca
    33541 ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca tagtccagca
    33601 gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta tgataccata
    33661 ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct acgcatttag
    33721 aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc atgccggaaa
    33781 agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt atcttttccg
    33841 ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc agtatttggc
    33901 ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca cttcgtcccg
    33961 gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg ccttaaacca
    34021 gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc cgtcgtccag
    34081 caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc gggccttgcg
    34141 atattgaggg taattcattc gtggaatact catagctctt gcgtccattc ctttcccggc
    34201 gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg catctgcttt
    34261 ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg cttgtactcg
    34321 gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag ggcctttgcc
    34381 gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc cttatccgct
    34441 gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat attctttatc
    34501 agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc ggcctcgtcg
    34561 ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc tatcccatgt
    34621 tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc
    34681 tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg caaagaaatc
    34741 cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc tttgatggct
    34801 tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg tccgggagcg
    34861 cggaaagaaa taaactttcc cggcttgatc tcatagccct gcgcctgcat gagccgcagg
    34921 aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct
    34981 ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc cttactctgg
    35041 ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt gcgccggata
    35101 taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc cacggcacag
    35161 aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac atagggatat
    35221 ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg ttccggcgtg
    35281 gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc cttttggtat
    35341 gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca ggcaaaggag
    35401 gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag ggcttttttc
    35461 agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc gcccccttta
    35521 tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt gcaatctgat
    35581 tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca
    35641 tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag ttcttcgtat
    35701 gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca gggacgcgca
    35761 gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca ggggtttggg
    35821 gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc cgtttttccg
    35881 ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca agacgtttcc
    35941 aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa
    36001 gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca catcctccgt
    36061 ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct cgatgatcgg
    36121 caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca gttcgctttc
    36181 ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg ctttaataag
    36241 ctgtatgaca accggcagac tgggaatggc cccgtcgttt tcgatgttgg caagatagcg
    36301 ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc
    36361 cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat tcgccatatc
    36421 gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg tatatgcacc
    36481 tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa agaattataa
    36541 tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg cgagaaatgg
    36601 ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc cggcgctgta
    36661 aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga caggcgtaca
    36721 aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg gatatggttt
    36781 tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat agatggcagg
    36841 ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat ttacacatgg
    36901 atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa acaaagacga
    36961 ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca tctggattct
    37021 ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt gagtatgggg
    37081 tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg ggatttacat
    37141 ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat atgaggaata
    37201 tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt
    37261 gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa gccataccgc
    37321 aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg tccccaacaa
    37381 gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta aaggatattc
    37441 tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc
    37501 caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa accaataata
    37561 tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta aaatccgaaa
    37621 agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc ttaaaatgga
    37681 gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat ctgtaataca
    37741 gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga tggaaatgag
    37801 gcatacacca agttttacga aaacacttat caactggtta ttcttgatat tatgctgccc
    37861 ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg
    37921 atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatgc agaggcagac
    37981 gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga agccctgctg
    38041 cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact tctgccggag
    38101 gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga atttgaaatc
    38161 cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat tttgtcacgt
    38221 atatggggat atgactttga gagtgatggc agcacagtcc acactcatat caaaaatttg
    38281 cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata ccgattggag
    38341 gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat acgatcattg
    38401 tcctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt gtgtcttatt
    38461 tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg gaactgattc
    38521 agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac aataaccaat
    38581 cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca ccaaatgcca
    38641 atacatcaaa tagttttaga cccgactttc tttatgtggt acatagagat gataatattt
    38701 cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg acaattcggg
    38761 gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat atctttgcgc
    38821 ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg gcaaacctga
    38881 aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct catgatatgc
    38941 attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg
    39001 aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat gagctaaaaa
    39061 cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc ggtgactaca
    39121 aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca
    39181 tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc atagctgaac
    39241 cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc ttggcagagg
    39301 caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg tccgatccga
    39361 ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac acgccgcagg
    39421 gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt tttgttttga
    39481 acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca ttctatcgca
    39541 ttgatcaggc gcgaagcaga aaaagtgggc gaaacggttt aggacttgcc atcgtacaaa
    39601 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    39661 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttga
    39721 ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta
    39781 cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt
    39841 tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca
    39901 tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat
    39961 ttcgttcaca gtctgcacgc tgtttttggg ggtggagact ggcttccgta ttggaggcaa
    40021 cacagatacc gcccatccct gcaactcata caggcagcag cactgacgta gtggagaatt
    40081 tggaggaaaa cgctcttgcc accgccaaag aacagggaga tgaacaggaa tggagcctga
    40141 ttttagtgaa caggcagaac cccatccccg cacagtacga tgtggagctt gagcaactat
    40201 caaatggtga gcggatagat attcggattt ctccctatct tcaagatttg tttgatgccg
    40261 caagaactga tggagtttac ccgattgtcg catccggata ccgaacaaca gaaaaacagc
    40321 aagaaattat ggatgaaaaa attgccgaat ataaggcgaa aggctacacc tctgcacagg
    40381 ctaaagcgga agcagaaact tgggtggccg tgccgggaac gagcgagcat cagcttggtc
    40441 ttgctgtgga tatcaatgcg gacggaattc attcaacagg caacgaggtt tatagatggc
    40501 tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta cccaccagac aagacagaga
    40561 taaccggtgt gagcaacgag ccgtggcatt accgatatgt tggcatcgaa gctgccacag
    40621 agatgtacaa ccaaggggtt tgccttgagg aatatttaaa accagaaaaa tgagaaaagg
    40681 atgttatgtt atggacagaa aaagattgac acagcgtttc ccgttcctgc ttccaatgag
    40741 acgggcgcag agaaaaatgt gcttttatgc gggaatgaga tttgacggct gtcgctatgc
    40801 gcagacgata ggggaaaaat cgctttccca tttgctcttt gaaacggatt gcgcattata
    40861 caaccacaat accggatttg acatgatata ccaggaaaac aaggtgttca acttaaagct
    40921 ggcagcaaag accttaaacg gcttattgat aagaccgggg gaaacctttt ctttctggtg
    40981 gctggtacgc catgcggaca aagatacccc ctataaagac ggccttacgg tgaccaatgg
    41041 taaactcacc accatgtcgg gcggcggtat gtgccagatg agcaatttac tattttggat
    41101 gttcctgcat acgccattga caattatcca gcgcagaggt cacgaagtaa aggagtttcc
    41161 agagccaaac agtgacgaga ttaaaggggt ggatgcaacc atttcagagg gctggattga
    41221 tttaaaagtg cgaaacgata ccgactgcac ctaccaaata tgagtgacct tagatgatga
    41281 gaaaatcatc ggtcaggtgt ccgccgacaa agagccccaa gcattataca aaattacaaa
    41341 tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat gaatatgccc aggttaaacg
    41401 gatgcaagtt gccttaggta ccggggaaat aatagattgc aagctgcttt atacaaacaa
    41461 atgcaaaatc tgttatcccc tcccggaaag tgtggatatt
//
LOCUS       DACCSK010000010        40814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCSK010000010 DACCSK010000000
VERSION     DACCSK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.010"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IVP39_000479"
     CDS             23..646
                     /locus_tag="IVP39_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585577.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IVP39_000480"
     CDS             1096..2145
                     /locus_tag="IVP39_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7585578.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IVP39_000481"
     CDS             2142..3023
                     /locus_tag="IVP39_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585579.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IVP39_000482"
     CDS             3026..3796
                     /locus_tag="IVP39_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7585580.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IVP39_000483"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IVP39_000483"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7585581.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IVP39_000484"
     CDS             5107..6270
                     /locus_tag="IVP39_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7585582.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IVP39_000485"
     CDS             6387..6797
                     /locus_tag="IVP39_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7585583.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IVP39_000486"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IVP39_000486"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7585584.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKNEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IVP39_000487"
     CDS             9337..9654
                     /locus_tag="IVP39_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585585.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IVP39_000488"
     CDS             9814..10050
                     /locus_tag="IVP39_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7585586.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IVP39_000489"
     CDS             10047..10445
                     /locus_tag="IVP39_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7585587.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IVP39_000490"
     CDS             complement(11112..12056)
                     /locus_tag="IVP39_000490"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7585588.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IVP39_000491"
     CDS             complement(12352..13296)
                     /locus_tag="IVP39_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7585589.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IVP39_000492"
     CDS             13517..14731
                     /locus_tag="IVP39_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7585590.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IVP39_000493"
     CDS             14753..16768
                     /locus_tag="IVP39_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7585591.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IVP39_000494"
     CDS             complement(16851..18224)
                     /locus_tag="IVP39_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7585592.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IVP39_000495"
     CDS             complement(18250..18534)
                     /locus_tag="IVP39_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7585593.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IVP39_000496"
     CDS             complement(18536..20551)
                     /locus_tag="IVP39_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7585594.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IVP39_000497"
     CDS             complement(20730..21035)
                     /locus_tag="IVP39_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7585595.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IVP39_000498"
     CDS             complement(21124..22155)
                     /locus_tag="IVP39_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7585596.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="IVP39_000499"
     CDS             22362..23237
                     /locus_tag="IVP39_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7585597.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="IVP39_000500"
     CDS             complement(23389..24231)
                     /locus_tag="IVP39_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7585598.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IVP39_000501"
     CDS             complement(24323..26200)
                     /locus_tag="IVP39_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7585599.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IVP39_000502"
     CDS             complement(26197..27618)
                     /locus_tag="IVP39_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7585600.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IVP39_000503"
     CDS             complement(27621..31010)
                     /locus_tag="IVP39_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7585601.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="IVP39_000504"
     CDS             31267..32658
                     /locus_tag="IVP39_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7585602.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNGRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSNLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="IVP39_000505"
     CDS             complement(32731..33378)
                     /locus_tag="IVP39_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7585603.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="IVP39_000506"
     CDS             complement(33386..34072)
                     /locus_tag="IVP39_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7585604.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="IVP39_000507"
     CDS             complement(34078..35151)
                     /locus_tag="IVP39_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7585605.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="IVP39_000508"
     CDS             35373..36047
                     /locus_tag="IVP39_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7585606.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="IVP39_000509"
     CDS             36052..37392
                     /locus_tag="IVP39_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7585607.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="IVP39_000510"
     CDS             complement(37541..38527)
                     /locus_tag="IVP39_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7585608.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="IVP39_000511"
     CDS             39251..40282
                     /locus_tag="IVP39_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7585609.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..>40814
                     /locus_tag="IVP39_000512"
     CDS             40391..>40814
                     /locus_tag="IVP39_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7585610.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        12670 a         7411 c         7663 g        13070 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg tgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataatactg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagca
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggg ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaatgaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga acgaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacggtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc caatctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attc
//
LOCUS       DACCSK010000011        39363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCSK010000011 DACCSK010000000
VERSION     DACCSK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.011"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(5..1084)
                     /locus_tag="IVP39_000513"
     CDS             complement(5..1084)
                     /locus_tag="IVP39_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7585611.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1187..1627)
                     /locus_tag="IVP39_000514"
     CDS             complement(1187..1627)
                     /locus_tag="IVP39_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7585612.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(1684..2094)
                     /locus_tag="IVP39_000515"
     CDS             complement(1684..2094)
                     /locus_tag="IVP39_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7585613.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2274..2708)
                     /locus_tag="IVP39_000516"
     CDS             complement(2274..2708)
                     /locus_tag="IVP39_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7585614.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(2808..3656)
                     /locus_tag="IVP39_000517"
     CDS             complement(2808..3656)
                     /locus_tag="IVP39_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7585615.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4045..5811
                     /locus_tag="IVP39_000518"
     CDS             4045..5811
                     /locus_tag="IVP39_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7585616.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            5913..7172
                     /locus_tag="IVP39_000519"
     CDS             5913..7172
                     /locus_tag="IVP39_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7585617.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7319..8620
                     /locus_tag="IVP39_000520"
     CDS             7319..8620
                     /locus_tag="IVP39_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7585618.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            8623..9474
                     /locus_tag="IVP39_000521"
     CDS             8623..9474
                     /locus_tag="IVP39_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7585619.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9487..10302
                     /locus_tag="IVP39_000522"
     CDS             9487..10302
                     /locus_tag="IVP39_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7585620.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10453..11619
                     /locus_tag="IVP39_000523"
     CDS             10453..11619
                     /locus_tag="IVP39_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7585621.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            11849..12496
                     /locus_tag="IVP39_000524"
     CDS             11849..12496
                     /locus_tag="IVP39_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7585622.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            12580..13524
                     /locus_tag="IVP39_000525"
     CDS             12580..13524
                     /locus_tag="IVP39_000525"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7585623.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            13659..15110
                     /locus_tag="IVP39_000526"
     CDS             13659..15110
                     /locus_tag="IVP39_000526"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7585624.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15131..15967
                     /locus_tag="IVP39_000527"
     CDS             15131..15967
                     /locus_tag="IVP39_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7585625.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            15967..16626
                     /locus_tag="IVP39_000528"
     CDS             15967..16626
                     /locus_tag="IVP39_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7585626.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            16752..17441
                     /locus_tag="IVP39_000529"
     CDS             16752..17441
                     /locus_tag="IVP39_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585627.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17447..18403
                     /locus_tag="IVP39_000530"
     CDS             17447..18403
                     /locus_tag="IVP39_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7585628.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18447..18959
                     /locus_tag="IVP39_000531"
     CDS             18447..18959
                     /locus_tag="IVP39_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7585629.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19215..19763)
                     /locus_tag="IVP39_000532"
     CDS             complement(19215..19763)
                     /locus_tag="IVP39_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7585630.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(19852..21033)
                     /locus_tag="IVP39_000533"
     CDS             complement(19852..21033)
                     /locus_tag="IVP39_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7585631.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21269..22777)
                     /locus_tag="IVP39_000534"
     CDS             complement(21269..22777)
                     /locus_tag="IVP39_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7585632.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            22936..23127
                     /locus_tag="IVP39_000535"
     CDS             22936..23127
                     /locus_tag="IVP39_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585633.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23330..24247
                     /locus_tag="IVP39_000536"
     CDS             23330..24247
                     /locus_tag="IVP39_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7585634.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24240..25313
                     /locus_tag="IVP39_000537"
     CDS             24240..25313
                     /locus_tag="IVP39_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585635.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            25461..25979
                     /locus_tag="IVP39_000538"
     CDS             25461..25979
                     /locus_tag="IVP39_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585636.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            25960..26352
                     /locus_tag="IVP39_000539"
     CDS             25960..26352
                     /locus_tag="IVP39_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7585637.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26428..27363
                     /locus_tag="IVP39_000540"
     CDS             26428..27363
                     /locus_tag="IVP39_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7585638.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            27496..28275
                     /locus_tag="IVP39_000541"
     CDS             27496..28275
                     /locus_tag="IVP39_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7585639.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28268..28585
                     /locus_tag="IVP39_000542"
     CDS             28268..28585
                     /locus_tag="IVP39_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7585640.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            28829..30439
                     /locus_tag="IVP39_000543"
     CDS             28829..30439
                     /locus_tag="IVP39_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7585641.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            30596..31444
                     /locus_tag="IVP39_000544"
     CDS             30596..31444
                     /locus_tag="IVP39_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7585642.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            31675..32154
                     /locus_tag="IVP39_000545"
     CDS             31675..32154
                     /locus_tag="IVP39_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585643.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            32187..34415
                     /locus_tag="IVP39_000546"
     CDS             32187..34415
                     /locus_tag="IVP39_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7585644.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            34544..36187
                     /locus_tag="IVP39_000547"
     CDS             34544..36187
                     /locus_tag="IVP39_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7585645.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(36260..36805)
                     /locus_tag="IVP39_000548"
     CDS             complement(36260..36805)
                     /locus_tag="IVP39_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7585646.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(36828..37313)
                     /locus_tag="IVP39_000549"
     CDS             complement(36828..37313)
                     /locus_tag="IVP39_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7585647.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(37303..38115)
                     /locus_tag="IVP39_000550"
     CDS             complement(37303..38115)
                     /locus_tag="IVP39_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7585648.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(38254..>39363)
                     /locus_tag="IVP39_000551"
     CDS             complement(38254..>39363)
                     /locus_tag="IVP39_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7585649.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        12958 a         7113 c         7733 g        11559 t
ORIGIN      
        1 tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata
       61 catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc
      121 gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca
      181 tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga
      241 tataaacaac atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag
      301 aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca
      361 gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta tttaaaagag
      421 cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat
      481 agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa
      541 ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca
      601 ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct
      661 gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat
      721 cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat gtttgatgaa
      781 taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa
      841 tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat
      901 gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta
      961 agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca
     1021 aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat
     1081 tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt
     1141 tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag ccctttttct
     1201 ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta tccaattctt
     1261 cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct tgtttttgca
     1321 acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc gtttcacgct
     1381 gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc gtaccagaat
     1441 ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc cataaaacta
     1501 acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga gtgtagtgtc
     1561 taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact aaatatagat
     1621 cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt tacacaaaaa
     1681 agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag cttttgaata
     1741 cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag aaagaccttc
     1801 gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg aataaaggga
     1861 aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga tcaattctaa
     1921 tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt
     1981 ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg gattatgcac
     2041 ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt tcataaaatt
     2101 accaccttta attatatttt atacaattga attttacaca atataattgt ggaaagcaaa
     2161 gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg ctttctcgaa
     2221 tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa acattacata
     2281 tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac ctgatgaagc
     2341 atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa gtccatcatt
     2401 tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg tgtgtcctcg
     2461 atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt aggagcagtc
     2521 aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa ggtaaaaatg
     2581 acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc atcaaagatt
     2641 gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat ttccgttcct
     2701 ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct tttattatac
     2761 cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca ttcgattgga
     2821 tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac aaaggcactt
     2881 ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt cacaacgatc
     2941 aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag ggtaaggcca
     3001 cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc actcatgacc
     3061 ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat tgaagtgatt
     3121 gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag attaagagaa
     3181 acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa atatttttta
     3241 aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata taaaatcgca
     3301 actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc tgaagtaatc
     3361 attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa aagtacttta
     3421 aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat attcaacact
     3481 aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc tagaccgctg
     3541 acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac tcctaatatg
     3601 aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc tttcatttat
     3661 tcacttcctt aattcattaa taatactctt atcttattat aacttacttt tcaccttaag
     3721 ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca ttcttctaga
     3781 atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg gagaaattga
     3841 tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa cttttaaaag
     3901 ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa aaaactgtta
     3961 ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt gatgacgtca
     4021 tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg accggaaagt
     4081 gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac taggaaaaat
     4141 gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac tgaaaaatgg
     4201 tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga ttattgggaa
     4261 attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt tggggaagat
     4321 aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa tgtgattact
     4381 gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt cactattcca
     4441 gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc aaacggggac
     4501 aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga ccgagaagac
     4561 tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac ggaccttggc
     4621 atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa atacgatacg
     4681 attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa aaacttgatt
     4741 gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa tcatatgggt
     4801 gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat ctataaagac
     4861 tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt accagaaact
     4921 gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa actaaataca
     4981 gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat aaaagaattc
     5041 gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa
     5101 ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga gatttggcat
     5161 tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta tgcatttaca
     5221 gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga
     5281 atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt caacttatta
     5341 gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga attgatgaag
     5401 tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta cgggacagaa
     5461 atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg ggagaaggag
     5521 gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag gaaacagttg
     5581 tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg tgaagataag
     5641 ctctatttca cacggaaact agagggacag atcatctatg tttactttaa tcagtcaaaa
     5701 gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg tcagttgtta
     5761 gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg aaaactgtta
     5821 ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa taagggtgta
     5881 acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg
     5941 gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc aatggtaact
     6001 cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt gattctggat
     6061 ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca
     6121 agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat gggcctgctg
     6181 gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta ggacagcaag
     6241 gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca gacaaagccc
     6301 aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc cttgttttgt
     6361 actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg gagaatctat
     6421 ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga tttttagcta
     6481 aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc tatgtatttg
     6541 gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa
     6601 tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa
     6661 gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca atcattgacg
     6721 gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta gccaaaattc
     6781 ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga tgggtagtca
     6841 gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta acgaatcagg
     6901 aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg
     6961 aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa tatgaaacag
     7021 ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga
     7081 tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa
     7141 caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc atttttataa
     7201 aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat tatcttgctc
     7261 ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg gcattcagat
     7321 gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt cacttattcc
     7381 agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt tcgccatatt
     7441 cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag gattggttac
     7501 tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag gcactttgca
     7561 attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag acgcttatcg
     7621 agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg aagtcataca
     7681 taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc ttctcatgat
     7741 ttttacgatc attttcccag tcctagttac attgttcact gcatttacta attatgactt
     7801 caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact tttttagtat
     7861 cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg
     7921 gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca
     7981 ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac catgggcggt
     8041 cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg taggtgccat
     8101 caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca gtgcaatctc
     8161 ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg gctggttagg
     8221 atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag aagacttata
     8281 tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc
     8341 tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga atttcaataa
     8401 tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg
     8461 ttctactgat attcttattt cctggatcta taaacttacg actggaactt ctccgcagta
     8521 ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg tgtcattgct
     8581 ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa atttcattcg
     8641 agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct tttaatactg
     8701 tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa gtcaggaaat
     8761 attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt tactcggcta
     8821 ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc tgtcctaaca
     8881 atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg ctatcgtttt
     8941 atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc gactatggca
     9001 gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa gtactggttc
     9061 ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat gaaaggatac
     9121 tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc agggcatttc
     9181 aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt tcaagcatta
     9241 tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct tcgaacacct
     9301 gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac aaaccaaaga
     9361 gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct attattcttc
     9421 tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg ataggagcag
     9481 agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc aactcaagtg
     9541 tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt atttttagtt
     9601 tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc gattccatta
     9661 gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa attttctgaa
     9721 cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt gacccctgaa
     9781 attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac ttttattgat
     9841 tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg ccgctatacg
     9901 atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga tttccaacga
     9961 tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc ttatagttta
    10021 agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc ttcctttttc
    10081 ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga gagtgcaaat
    10141 ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg gtttatccac
    10201 tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct gcttttatgg
    10261 gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta agcgatttat
    10321 gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa
    10381 ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt agttttttta
    10441 aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg ttctttctcg
    10501 ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga tacggacact
    10561 cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat tacaagtaac
    10621 gcatctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga gggaagtatc
    10681 caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg aaaagattat
    10741 ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga agatatccat
    10801 gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac tttgacttat
    10861 tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat caataaagtc
    10921 catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt
    10981 ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc aactgggatc
    11041 acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga acatttagaa
    11101 gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac gtttgatcat
    11161 atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa
    11221 gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat cagccgacaa
    11281 caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg ttatgtccct
    11341 ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat tgatttggaa
    11401 agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt tcatcaagtc
    11461 caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga tctatattta
    11521 caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata tttgcttgtt
    11581 caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa gtactgttta
    11641 aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt ttttgtctaa
    11701 cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata ataaaagttt
    11761 ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg taatagaata
    11821 ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata ctagtttatt
    11881 tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat ctgggaatac
    11941 gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa aagcaagcac
    12001 atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag atatcaaagt
    12061 gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg caattactag
    12121 tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg tcgatgatca
    12181 aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag agaatccaga
    12241 aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc tggatgttga
    12301 taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac
    12361 ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag tcaaagtaaa
    12421 agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag tactgtcaac
    12481 tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt ttagtcatga
    12541 tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga aacgaccttt
    12601 gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg catgactacc
    12661 caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa gagctgtacg
    12721 atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt agggagtatc
    12781 aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc tcttgcgtat
    12841 gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg gatgagtgtc
    12901 caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa
    12961 ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga ttttgaggcc
    13021 acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg aatcaaattg
    13081 cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa tcagtttcca
    13141 ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga tacagaacaa
    13201 gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga gtatatcaag
    13261 ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc
    13321 attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct atgtggcgca
    13381 agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt ttctagaatc
    13441 ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga cgaatttaaa
    13501 ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc ttcgtctttt
    13561 tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta tgttagacga
    13621 acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca acagaattag
    13681 caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat atacagaaaa
    13741 aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta
    13801 attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt atcccatttc
    13861 ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga tctcgtttat
    13921 ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa gcgataggtc
    13981 tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt acagacccag
    14041 tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc ggatcaagtg
    14101 gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct agtgatggcg
    14161 gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag
    14221 ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct attgatttcg
    14281 gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga agtatccatt
    14341 ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga aaaaaacggt
    14401 tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa gaggcagtac
    14461 ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg acagagattc
    14521 cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat ggcgcagaaa
    14581 cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga aaagaagaac
    14641 ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc gctacttaca
    14701 tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta catcaagaat
    14761 acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc gaagatttgc
    14821 aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa gtagaaggtt
    14881 tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat acacagttgg
    14941 ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa gaaaacatgc
    15001 ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt caaatcggct
    15061 atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag cagaaacaag
    15121 gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct tttttatttt
    15181 aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta atgtagaaaa
    15241 aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt attataatga
    15301 tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta aacggttaaa
    15361 tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg tttcaaaaag
    15421 agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa aaaaattctc
    15481 cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa aagatgattc
    15541 aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac ccaatactag
    15601 ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca atgccacgaa
    15661 tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa atgattatta
    15721 tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac tggagaacgt
    15781 ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa
    15841 gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga aaatatctga
    15901 aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg agtcggtgag
    15961 tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact tcctttcgta
    16021 ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt aggaaatctg
    16081 gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa tgtatttatt
    16141 cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt tctgttatat
    16201 tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt tacgattacc
    16261 agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc ttcaggtcag
    16321 tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata tatcgttttg
    16381 ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga tctagtgaaa
    16441 ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc aaagatcata
    16501 ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat ttattgggtc
    16561 atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat gcatcagtca
    16621 atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt tgtttcttgt
    16681 atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg
    16741 aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta taccaatatt
    16801 gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac caaggaattg
    16861 tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga tgctatgagc
    16921 tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt ggacattata
    16981 attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc cctacaggaa
    17041 taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc tctggtacat
    17101 ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa gctttgacaa
    17161 accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg
    17221 taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca gaaaatcaag
    17281 atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct agtcaggcaa
    17341 tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac
    17401 aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt actatcctta
    17461 tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc gaggattgct
    17521 ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt
    17581 gatcgaatta tttcaaaaaa agcaccatcg aatcgctgtg atccaaaatc cccaagtagg
    17641 acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa atagctctgt
    17701 cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt cacctccagc
    17761 gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga aacagttggc
    17821 aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct ggctgcccga
    17881 aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa gttatgcaga
    17941 taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg gatatattgg
    18001 attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc ctagccgagc
    18061 gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga aacattttgt
    18121 ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag caggagataa
    18181 aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag gaatgattga
    18241 attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga aaaagtggtc
    18301 acttatgcat catttggaac ttattagtca cctgctagat catccaaaag attggcttaa
    18361 atattattca aaagttcagg aactatatga agtgatccaa tgattagata atgtaaatga
    18421 aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa gaagaagtag
    18481 aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa ggctctcctc
    18541 aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag
    18601 aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta gtctcaagcg
    18661 tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt ccttatttat
    18721 cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca aaaccgttct
    18781 tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt atcgatactt
    18841 atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact tatcaaggga
    18901 tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc atcaattgac
    18961 tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac aatcatatga
    19021 tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa
    19081 tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct ttctgaaact
    19141 cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta tcctacgagt
    19201 attattcgat acatttatat caataatcct ttttttattt tatctgcagc ttcttggaac
    19261 gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt caactgtgct
    19321 aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc tccacgtttt
    19381 ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg gactaccgtc
    19441 ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta acaatttcct
    19501 actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg agcatattgg
    19561 tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc agaaacaaaa
    19621 taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta agtaatgatc
    19681 ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc agaaggaccg
    19741 ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca agtgaaaaag
    19801 ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat attataccag
    19861 caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca aaattttcaa
    19921 tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg caccagaaat
    19981 gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa ttgctgttgt
    20041 acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa attcatttgt
    20101 aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg ggattccgct
    20161 gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga tgccgaatat
    20221 cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa gcatagcagc
    20281 aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt ggaaaaatgt
    20341 ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata catagggatt
    20401 tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag tgatcacata
    20461 tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg tagacatagc
    20521 cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct tttttatgga
    20581 aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag attccaattt
    20641 gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata atggaagaat
    20701 cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca tcaatacgga
    20761 aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt caacaccttg
    20821 cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa ccgctgtacc
    20881 aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca tcacaattag
    20941 tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac
    21001 aaataaacca ataataccta ataaataact caaataatca ctcctattcc aaattccgtt
    21061 ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca ttactttttt
    21121 agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa aggtttaaaa
    21181 agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc taatagtttc
    21241 gttttcaaca tttataagta aactttttct attggtaata tagattttcg gaagggtaga
    21301 caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga
    21361 ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt
    21421 gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta
    21481 gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta
    21541 atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga
    21601 taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg
    21661 gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat
    21721 ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt
    21781 ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc
    21841 attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat
    21901 catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag
    21961 attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc
    22021 gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga
    22081 catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa
    22141 cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt
    22201 catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca ggagctgtca
    22261 ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga
    22321 cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt
    22381 ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga
    22441 taacgacact gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat
    22501 ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat
    22561 agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat
    22621 cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc ttcccaccaa
    22681 attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt
    22741 caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa
    22801 agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac
    22861 aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga tagtttttga
    22921 taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc
    22981 cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg
    23041 tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc
    23101 acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg
    23161 tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc
    23221 ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta
    23281 ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc
    23341 agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg atcaggatac
    23401 aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca
    23461 attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat
    23521 ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca
    23581 cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt
    23641 tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta
    23701 ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa
    23761 gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat
    23821 caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca
    23881 aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg
    23941 taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga
    24001 aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc
    24061 attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag
    24121 gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt
    24181 gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta
    24241 tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag
    24301 aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc
    24361 ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg aatacgaata
    24421 ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag
    24481 agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt
    24541 tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc
    24601 gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact
    24661 tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt
    24721 atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat
    24781 tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc
    24841 tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt
    24901 tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc
    24961 acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat
    25021 tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa ttgacgaatg
    25081 aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga
    25141 tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa
    25201 acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg
    25261 atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata
    25321 agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc
    25381 agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa cttgctgatg
    25441 aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc ttattgtctg
    25501 ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag ccaatgactg
    25561 ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc
    25621 tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc ggccaatgga
    25681 tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa
    25741 ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac ggaataggta
    25801 tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat taccttcaag
    25861 gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt gtttttctgc
    25921 cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt
    25981 ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac ctatacacaa
    26041 tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa
    26101 gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg
    26161 agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa
    26221 gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga cgggaaaagt
    26281 gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat tttcaatttg
    26341 tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata attcagtgag
    26401 ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg aatgttttcc
    26461 gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt cggctatgca
    26521 atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga ttgcagcaat
    26581 cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga gttgcatcaa
    26641 tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat gcttttcttc
    26701 ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc atcccatgaa
    26761 gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat cgaaggaagt
    26821 tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact ttccacgatg
    26881 cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga agacttgtgt
    26941 gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc aaccgggatt
    27001 gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg agccgccatt
    27061 ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc aagcgattta
    27121 tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga tataaaaaca
    27181 gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt tggtaacaaa
    27241 gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca gtctcttcct
    27301 gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta ttctcgtaaa
    27361 taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg tttttaaata
    27421 aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt tgttgtaatt
    27481 aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca
    27541 gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt attgttgcaa
    27601 aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt ttccggtggc
    27661 gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac gacattattg
    27721 atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc cggttttatg
    27781 aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga tgaaaactat
    27841 gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa ggctttatat
    27901 gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc
    27961 tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat gttcattttc
    28021 atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt ctcaggcgta
    28081 ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc agcaacagtc
    28141 attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa agctaagaaa
    28201 ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga acaggaggcc
    28261 aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca tcgtggcata
    28321 tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat cctggttcag
    28381 tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta aagatgtttt
    28441 cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa tgattctagt
    28501 agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag gacttgcgtc
    28561 tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga gattgcgaca
    28621 taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct ggatgcaagt
    28681 ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt ttttatttta
    28741 ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa gatgtttatt
    28801 ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag gaacattttg
    28861 gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga ttccctggct
    28921 aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata atgttggcta
    28981 tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa
    29041 aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga tttttagaag
    29101 tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt atttatttgc
    29161 tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac tagctatcag
    29221 aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag gatatttcca
    29281 aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg ctgtgcgagt
    29341 gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa ccatattaga
    29401 agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga ttatccatat
    29461 gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga tttccagccg
    29521 ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa cactaccgtt
    29581 tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc
    29641 actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct tattcagccg
    29701 tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag gaacagtagc
    29761 tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa
    29821 aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat tgactggaat
    29881 gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg aaagtgtcgg
    29941 atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga tcctatctac
    30001 gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag aagcaattat
    30061 cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg ggatgagctt
    30121 atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga
    30181 ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt tccgctccac
    30241 cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc gattgtcaat
    30301 ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg gcggcttagt
    30361 atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca
    30421 tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa aaacggcaac
    30481 tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat ctaatcttac
    30541 ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta aacacatgaa
    30601 acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga accatcccga
    30661 cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg aaggaatcga
    30721 tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc ctactacttc
    30781 acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga aagggtatga
    30841 cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca cattatttgc
    30901 gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga tcactagtat
    30961 gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc agtcccttga
    31021 tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc tgattgtaga
    31081 tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc tgattggcag
    31141 cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa
    31201 aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta gaagaaataa
    31261 cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag aagtagcgat
    31321 tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt
    31381 cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca tttcagcgca
    31441 ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt ggactttcta
    31501 catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat caatgaagct
    31561 ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt tagtaaatga
    31621 gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa aataatggaa
    31681 ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga taaagttgat
    31741 ggaagaaatg cattaaatac ggatgcaaaa gggctttcac tgggccttgc tcagtggaat
    31801 gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga aaaatggtca
    31861 cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt agctgcacaa
    31921 ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc agaaaatcca
    31981 gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga taccaaaaat
    32041 ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga cggagaactg
    32101 attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata ttaatagaca
    32161 gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa aaccgaacct
    32221 tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg acaagtgcag
    32281 aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa atgaaaatca
    32341 gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc aaagcactat
    32401 ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg gacaaatcag
    32461 atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac caagaattac
    32521 tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc cagaaccatt
    32581 acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa aggatcgaag
    32641 ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt cgagcgattg
    32701 aaaaaggata tttattagag attcaagagc cgactgtcat ccgagatgcc tctgttcttg
    32761 tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca ggaatcattc
    32821 gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa ggaaatcgcc
    32881 cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat cttcctgcct
    32941 tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa gaacttctct
    33001 taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac gccatcaaag
    33061 gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa ggacaagatt
    33121 tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca gatgaattgg
    33181 caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag gacatattga
    33241 gaaaagaaaa atggggtcat cctcaatctg agcatacaaa aggaagaacc attagcgccg
    33301 aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc ttagatgaaa
    33361 agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa gaaacaatag
    33421 aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc gaaggaaaaa
    33481 atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct gacatggaag
    33541 cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc atgaaggaaa
    33601 cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga aaaacgccgg
    33661 aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc cggcaaattt
    33721 tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac gaaggtcagc
    33781 ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa aagaacccgc
    33841 cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg tcaatgatac
    33901 gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa ttttctaaga
    33961 aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca agagaaaaga
    34021 cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat gaaaaattag
    34081 tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta gcagcagaaa
    34141 aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac ggtcagccaa
    34201 aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct gttttgaaag
    34261 aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac aaagaagaaa
    34321 tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat gaatttccca
    34381 agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa cgaacgcact
    34441 tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact tctaaaatag
    34501 aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac aagaacaaaa
    34561 atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag atagcaatgg
    34621 cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga aagatcttgg
    34681 tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata atggttatga
    34741 cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt ttgatttatt
    34801 gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca tcaaccatag
    34861 ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc cgtatcgcga
    34921 ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga catcgatttt
    34981 cggcggttct gcttgggagt attctcatga aactggacag tattatctgc atgtttttgc
    35041 aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac tattcaatat
    35101 gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga tttctcatgt
    35161 aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct ttcaaaatgt
    35221 ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt acgatatcat
    35281 gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg tagggaaaga
    35341 cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc aagaaaaaga
    35401 agggcaatta gatgtcctta aattgaaact tgcattaagc gcttggcaaa cttcattgga
    35461 tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg ccgtttcagt
    35521 gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg ctatgatgta
    35581 tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa tgacgaatat
    35641 gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt ataaaaggct
    35701 gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa caacgagaga
    35761 caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt ctacacatga
    35821 accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac aagaatatga
    35881 accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa ccaataaagg
    35941 tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt atgtctatga
    36001 aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat cacttgctga
    36061 ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa attcctctag
    36121 cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat ataaaacaaa
    36181 caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt ttttcagatt
    36241 gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat ttaggacgag
    36301 agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg acaggtactt
    36361 ggacatgttt gatatgcata ccgattgatg tgtcaccgat atcgataccg gcttgtccaa
    36421 ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca gaacaagcac
    36481 ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct tctgccactg
    36541 ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct agcgcaattt
    36601 ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact tccgcactgg
    36661 aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa acaaaaagat
    36721 cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc aattggtctt
    36781 ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta atattgcttc
    36841 cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat tccaatcaga
    36901 ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata taaaatccaa
    36961 ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc taatccccac
    37021 atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc gtggatcact
    37081 aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag accactggaa
    37141 gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata gatcccggcc
    37201 tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt taatgaaagg
    37261 gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt catttctcat
    37321 tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca tcagcacgcc
    37381 attagaaata caatgggcaa caaatttttt actttctgct actgctttgg taaaatctgc
    37441 ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc cgtttactgt
    37501 tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat agagaagatc
    37561 cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt ttgttcctga
    37621 agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa gagcttgacc
    37681 agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg gaaaaagttc
    37741 ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt tgaatgccaa
    37801 aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc gaacaaccat
    37861 gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag tcattttatc
    37921 aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca taaatcgctc
    37981 attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac caaaattctc
    38041 aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac cgccaattgt
    38101 caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat tttcaaaaaa
    38161 gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa aaagaaaaga
    38221 aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt ttaattcttt
    38281 ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat caaaatcacg
    38341 gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc gattccaatc
    38401 catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt tatcccttac
    38461 aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa tagctaatgt
    38521 actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt ggccatgctt
    38581 atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt cctggaaccg
    38641 tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta tatgaccatc
    38701 tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg tcatgtcttc
    38761 cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg tatgagcctt
    38821 aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg catcctgtgc
    38881 catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa aagtggagtg
    38941 atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac gccactgaac
    39001 aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca tatgaacaac
    39061 ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca tcaaagaaga
    39121 gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta atttcttttc
    39181 cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag aatgagcaga
    39241 atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat aagaaatttc
    39301 atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta aataaagaat
    39361 agg
//
LOCUS       DACCSK010000012        38826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCSK010000012 DACCSK010000000
VERSION     DACCSK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.012"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..648
                     /locus_tag="IVP39_000552"
     CDS             <1..648
                     /locus_tag="IVP39_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7585650.1"
                     /translation="QNPTGKVMSQKRREKLVELCRQYRLPIIEDDVFTDLSFLNQEEV
                     LPIRTIDPDNVLYVGSLSRVLGKTTKIGWVIGPETFISQLAQAQEMMEFSMNILTQIT
                     VANVFNEAIDLKMQDVRKKLKEKSRLISAWGDSQSFFKLHSILGGYYAWITWDGKKLD
                     PELAEKVVDMGLGIAPSLLFGQDINGIRINYSRLDENQIPLFYSKMKILEKWLTE"
     gene            1109..2218
                     /gene="pdhA"
                     /locus_tag="IVP39_000553"
     CDS             1109..2218
                     /gene="pdhA"
                     /locus_tag="IVP39_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7585651.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            2221..3198
                     /locus_tag="IVP39_000554"
     CDS             2221..3198
                     /locus_tag="IVP39_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7585652.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            3223..4518
                     /locus_tag="IVP39_000555"
     CDS             3223..4518
                     /locus_tag="IVP39_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAP7585653.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4526..5932
                     /gene="lpdA"
                     /locus_tag="IVP39_000556"
     CDS             4526..5932
                     /gene="lpdA"
                     /locus_tag="IVP39_000556"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7585654.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            6094..6369
                     /locus_tag="IVP39_000557"
     CDS             6094..6369
                     /locus_tag="IVP39_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7585655.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            6375..7142
                     /locus_tag="IVP39_000558"
     CDS             6375..7142
                     /locus_tag="IVP39_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7585656.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            7310..9139
                     /gene="typA"
                     /locus_tag="IVP39_000559"
     CDS             7310..9139
                     /gene="typA"
                     /locus_tag="IVP39_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7585657.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            9400..11172
                     /gene="recQ"
                     /locus_tag="IVP39_000560"
     CDS             9400..11172
                     /gene="recQ"
                     /locus_tag="IVP39_000560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7585658.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            11286..11588
                     /locus_tag="IVP39_000561"
     CDS             11286..11588
                     /locus_tag="IVP39_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7585659.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11591..12754
                     /locus_tag="IVP39_000562"
     CDS             11591..12754
                     /locus_tag="IVP39_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7585660.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12780..16208
                     /locus_tag="IVP39_000563"
     CDS             12780..16208
                     /locus_tag="IVP39_000563"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7585661.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            16229..17353
                     /locus_tag="IVP39_000564"
     CDS             16229..17353
                     /locus_tag="IVP39_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585662.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            17343..17780
                     /locus_tag="IVP39_000565"
     CDS             17343..17780
                     /locus_tag="IVP39_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7585663.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17764..18102
                     /locus_tag="IVP39_000566"
     CDS             17764..18102
                     /locus_tag="IVP39_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7585664.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            18221..18781
                     /gene="rsmD"
                     /locus_tag="IVP39_000567"
     CDS             18221..18781
                     /gene="rsmD"
                     /locus_tag="IVP39_000567"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7585665.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18778..19269
                     /gene="coaD"
                     /locus_tag="IVP39_000568"
     CDS             18778..19269
                     /gene="coaD"
                     /locus_tag="IVP39_000568"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7585666.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            19259..20302
                     /locus_tag="IVP39_000569"
     CDS             19259..20302
                     /locus_tag="IVP39_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7585667.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20461..21189
                     /locus_tag="IVP39_000570"
     CDS             20461..21189
                     /locus_tag="IVP39_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7585668.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            21305..21550
                     /locus_tag="IVP39_000571"
     CDS             21305..21550
                     /locus_tag="IVP39_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7585669.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21547..21765
                     /locus_tag="IVP39_000572"
     CDS             21547..21765
                     /locus_tag="IVP39_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7585670.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21901..22440
                     /locus_tag="IVP39_000573"
     CDS             21901..22440
                     /locus_tag="IVP39_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7585671.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22433..24706
                     /locus_tag="IVP39_000574"
     CDS             22433..24706
                     /locus_tag="IVP39_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7585672.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24744..25790
                     /gene="holA"
                     /locus_tag="IVP39_000575"
     CDS             24744..25790
                     /gene="holA"
                     /locus_tag="IVP39_000575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7585673.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25879..26133)
                     /locus_tag="IVP39_000576"
     CDS             complement(25879..26133)
                     /locus_tag="IVP39_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7585674.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26533..26802)
                     /locus_tag="IVP39_000577"
     CDS             complement(26533..26802)
                     /locus_tag="IVP39_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585675.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(27272..27727)
                     /locus_tag="IVP39_000578"
     CDS             complement(27272..27727)
                     /locus_tag="IVP39_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7585676.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27865..29793)
                     /locus_tag="IVP39_000579"
     CDS             complement(27865..29793)
                     /locus_tag="IVP39_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7585677.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(29990..30823)
                     /locus_tag="IVP39_000580"
     CDS             complement(29990..30823)
                     /locus_tag="IVP39_000580"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7585678.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(31015..31719)
                     /locus_tag="IVP39_000581"
     CDS             complement(31015..31719)
                     /locus_tag="IVP39_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7585679.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(31742..32368)
                     /locus_tag="IVP39_000582"
     CDS             complement(31742..32368)
                     /locus_tag="IVP39_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7585680.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            32694..35069
                     /locus_tag="IVP39_000583"
     CDS             32694..35069
                     /locus_tag="IVP39_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7585681.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(35093..35584)
                     /locus_tag="IVP39_000584"
     CDS             complement(35093..35584)
                     /locus_tag="IVP39_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7585682.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            35771..36691
                     /locus_tag="IVP39_000585"
     CDS             35771..36691
                     /locus_tag="IVP39_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7585683.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            36845..37189
                     /locus_tag="IVP39_000586"
     CDS             36845..37189
                     /locus_tag="IVP39_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7585684.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(37379..37879)
                     /locus_tag="IVP39_000587"
     CDS             complement(37379..37879)
                     /locus_tag="IVP39_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7585685.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            38407..38490
                     /locus_tag="IVP39_000588"
     tRNA            38407..38490
                     /locus_tag="IVP39_000588"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38441..38443,aa:Leu,seq:cag)
BASE COUNT        12935 a         6768 c         8008 g        11115 t
ORIGIN      
        1 caaaacccta caggaaaagt gatgagtcaa aaacgacgag aaaagctagt agaattgtgt
       61 cgacagtatc ggcttccaat tattgaagac gatgtattta cggatctttc atttctaaac
      121 caagaagaag tccttcctat acgaacgatt gatccggata atgtgttata cgtcggttcg
      181 ttatctaggg ttttaggaaa aacaacgaaa atcggttggg tgataggacc tgagactttt
      241 atctcgcaac tagctcaagc gcaagaaatg atggagtttt cgatgaatat ccttacacaa
      301 atcacagtgg ctaatgtatt taatgaagca attgatttga aaatgcaaga tgtaagaaaa
      361 aagttgaaag aaaaaagccg actgataagc gcttggggag attcacaatc gtttttcaag
      421 cttcattcaa ttttaggtgg ttactatgcc tggattactt gggatgggaa aaaactagac
      481 cccgaactag cagaaaaagt agtagatatg ggtcttggaa ttgcaccaag tctacttttt
      541 gggcaagata taaatggaat cagaatcaat tacagtaggc tggatgaaaa ccaaataccg
      601 ttgttttatt caaagatgaa aattctagaa aagtggttaa cagaataatt tcacaaagta
      661 aatagattaa agaatataac agcttggatt taaatttcaa gctgtttctt ttttttcgga
      721 aaaagaatac gatataaaga aactaagaaa aaaccattat ttgtacgtcc gttcacgatg
      781 tgtaaataaa ataaatgttt gcaacaaaac tgtataacac caacgttttt aaatagaaac
      841 agatggttgt aagtttgatt agaaaataga aaaaatgcag atgaaatata aaacgctttc
      901 cttattgtga ttttattcac taatgctgtt aatatgcgga ttggcataat ttcctttcat
      961 cgtgaaaatt tgtgctttta gcttgtattt ctcataaatc catgataatt tattaatgaa
     1021 gacggttaaa tagtgataca gttttattca ctgtttcaat cgcagaatga agagattttc
     1081 ttatagatga gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca
     1141 agcactgcta gatgaagtca atgcagattt cccagtatat caagcacttg atcaagatgg
     1201 aaacgtagtt aacaaagatt tagtacctga tttatccgat gatgaactcg ttgaattaat
     1261 gactcatatg gtatggtctc gtgtattaga ccaacgttca acagcgctga accgccaagg
     1321 acgcctaggc ttttttgcac caactgctgg acaagaggca agtcagctgg ctagtgctta
     1381 cgcatttgat aaagaagatg ttcttcttcc aggttatcgg gatgtacctc aactagtaaa
     1441 acatggttta cctttatctc aagctttttt atggtctcga ggacatgctg caggtaattc
     1501 ttatccggaa gaattgaaag ctctaccacc acaaattatc atcggtgcac aatatgtaca
     1561 agcagccggt gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta
     1621 tactggtgat ggcggttcat cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc
     1681 ttaccatgca aatgccgtat tttatatcca aaacaacggg tatgcgatct ctacacctcg
     1741 tgaaaaacaa actgcagcaa aaacacttgc acaaaaagct gtcgcagctg ggattcctgg
     1801 tattcaagtc gatggaatgg atccgcttgc agtatatact gtatctaaaa tggcacggga
     1861 atgggcgatc agtggaaatg gaccagtcct aatcgaaaca ctgacttatc gttatggacc
     1921 acatacacta tctggtgacg atccaacccg ctaccgtaca aaagacacag atgacgattg
     1981 gcagaaaaaa gatccattga tccgcttccg taaatatttg acggaaaaag gactttggtc
     2041 tgaagaaaaa gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag cagctattgc
     2101 tgaagccgac aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt
     2161 atctccacaa tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt cgaaataatc
     2221 atggcacaaa aaacaatgat ccaagcaatc acagatgcct tagcacttga actagaaaat
     2281 gacgagaatg tcgttgtttt tggtgaagat gttgggaaaa acggaggagt tttccgggca
     2341 actgaaggat tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc tttagctgaa
     2401 tctggaatcg caggattgag tttcggtctt gcgttagaag gatttcgtcc agttcctgaa
     2461 atccaattct ttggttttat tttcgaagca atggacgaag tagtagctca aatggctcgt
     2521 acaagatatc gaatgagcgg cactagaaat ctacccatca caattcgttc gccatttggt
     2581 ggcggtgttc atacaccaga attgcattcg gataatttag aaggtttgat cgctcagtct
     2641 cctggtattc gagtagtgat tccttccaac ccttatgatg caaaaggact attgatttct
     2701 tctattagaa gtaacgatcc ggttgttttc ctagaacaca tgaaactata tcgctcattc
     2761 cgagaagaag tgccggatga agcttacgaa gttcctttgg ataaagcagc agttactcgt
     2821 gagggaacag atgtatctat tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca
     2881 gcagacaatc ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg tacagttgcg
     2941 ccattagatg tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc
     3001 caagaagcac aacgacaagc gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc
     3061 gctatccttt cattagaagc cccaatcgga cgagtttctg cacctgatac agtcttccca
     3121 tttggacaag cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa
     3181 gaaatcgcgg aattttaaga aaagaaggga cgaaacaaag tcatggctta tcaatttaaa
     3241 ttgccggata tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca
     3301 ggagatacga tcaatgaaga tgatacatta ctagaagttc aaaatgacaa atcagtagaa
     3361 gaaattccgt cacctgtcac agggacagtt aaaaacgtga ttgttccaga aggcactgta
     3421 gcaaatgttg gggatgtatt agtagaaatc gatgctcctg gacacaatag tgcaccgtca
     3481 actagtgcgc catcagctga agcaccaaaa gaaaaagtgg aaacatcagg ttcagcaagt
     3541 gtcgttgaag ctgccgatcc aaataaacgt gtattggcta tgccatctgt tcgtcaattt
     3601 gcccgtgaaa aagatgtaga tatctcacaa gtaactgcaa ctggaaaagg tggcagagta
     3661 acaaaagagg acatcgaaaa cttcttggca ggtggtccaa gcagtgcacc agcgaaatca
     3721 gaagcgccag aagcggcagc accgaaagaa gcagctcctg cagcagaaag caaaccagct
     3781 gcaccagcaa aaccattcaa gtccaactta ggagacttgg aagaacgtgt agcaatgacg
     3841 ccaacacgta aagctatcgc taaagcaatg gtcaacagta agcatacagc tccacacgtg
     3901 acattgcatg atgaagtaga agtgtctaaa ctatgggata atcggaaacg atttaaagaa
     3961 gttgctgctg caaatggtac gaaacttact ttcttgccat atgtcgtgaa agctttaaca
     4021 gcgactgtta agaaataccc agtattgaat gcatctatcg acgatgcaaa ccaagaaatc
     4081 gtttataaac attactacaa tatcggtatt gcaactgata cggatcacgg actatatgta
     4141 ccaaatgtga aagatgcaga ccgcaaagga atgtttgcta ttgcagacga aatcaatgag
     4201 aaagcaaaat tagctcatga cggcaaacta tctgcagaag atatgcgcaa tggaacaatt
     4261 acgatcagca atatcggatc tgtaggtggc ggttggttta caccagtcat caactatcca
     4321 gaagtagcta ttttaggtgt aggaacgatt gcacaacaac caatcgtaaa tgctgaaggc
     4381 gaaatcgttg tcggtcgtgt gatgaaactg tcattaagtt tcgatcaccg tatcgtagat
     4441 ggagcaactg cgcaacaagc aatgaataat atcaaacgtc ttttagctga tccagagcta
     4501 ttaatgatgg aaggatgata aacaaatggt agttggagat tttgctgtag aattagatac
     4561 agttgtaatt ggtgcgggac ctggtggcta tgtagctgcc atccgcgctg ctgaaatggg
     4621 acaaaaagta gcaatcattg aaagagagta tattggcggt gtttgtttga atgtcggctg
     4681 tattccttca aaagcactga tagctgcagg acaccattat caagagtcac tagattctga
     4741 aatgtttgga gtaactagtg aaaacgtcaa actagatttt gcaaaaacac aagaatggaa
     4801 agaaaacaaa gttgtccata cgttgacttc tggtgttggt ttcctattaa aaaaacataa
     4861 ggttgaaaca atcgaaggag aagcgttctt cgtagatgat cacacactac gcgtcattca
     4921 tccagattca gctcaaactt attcgttcaa taacgcaatc atcgcaacag gttcccgtcc
     4981 aatcgagatt ccaggattta aattcggagg acgtgtgtta gattctactg gcggattagc
     5041 attgaaagaa gtacctaaga aatttgtgat catcggtggc ggtgtgattg gtgccgaatt
     5101 gggcggagcg tacgcaaact taggagcaga agtaacgatt cttgaaggct caccacaaat
     5161 tttaccaact tacgaaaaag atctagtaaa attagtagaa gatgatttca agaaaaaagg
     5221 cgtaacagtc gtaacgaatg caatggcgaa agaagcagtc gataacggtg acagcgtgac
     5281 agtgaaatat gcagtggacg ggaaagaaga atccgttact gctgattatg tgatggtcac
     5341 tgtaggtcgt cgtccgaata cagatgatat gggcttagaa caagcgggtg tcgaagtagg
     5401 agaacgagga ttgatcacgg tggataaaca aggacgtacg aacgtaccta acatctatgc
     5461 catcggtgat atcgttccag gggcggcatt agcacacaaa gccagttatg aagcaaaaat
     5521 cgctgctgaa gcaatctctg gcaaaaaagt agctgtagat tataaagcaa tgccggcagt
     5581 agccttcact gatcctgaat tggcttctgt aggtatgacg atcaaagaag caaaagatgc
     5641 tggtatcgaa gcaaaagcat ataaattccc attttcaggg aacggtcggg cattatcgct
     5701 tggtaaaaca gaaggattca tccgtctagt tacgacgatc gaagataacg tgcttattgg
     5761 cgcgcaaatc ggtggagtcg gcgcaagtga tatggtctct gaattagctt tagcaatcga
     5821 atcaggaatg aatgctgaag atatcgcctt gacgattcat cctcatccat ctcttggcga
     5881 aatcgtcatg gatgcttcgg aattagcatt aggtttacca atccatattt gatttgaata
     5941 atacaggcga aacgcaactg aaaaatcagt tgcgttttgt tttttagaat tatcaataat
     6001 attattatgt aaacttaaaa atatgacaac ttcttggttt attaccactt cctatgctac
     6061 actgaagagg aatatgaaat aggagagttg aagatgcaag aatatcagta tccacttgat
     6121 ttagattgga caacagacga aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt
     6181 gcgaatgaaa aagggataaa caatgaagct tttttgaaga cttatcagca gtttaaaact
     6241 gttgtcaaat ccattggtga ggaaaaacga ttgggacggg aatttgaaaa agcttccggt
     6301 tattctttat atcgtacagt gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg
     6361 aacgggtgag ataaatggaa cagatgattg atcatatcaa gtcctggctt gaagaagctg
     6421 gtgagaaaat caaatcttct caacaaacat taacaatctc gcaaaaatca aatcgaacgg
     6481 atctagtaac aaatgtcgat cgaaacatcc agaattttct aatcgaaaaa atccattcct
     6541 ttgatccaga ggcgaaaata ttggcagaag aagaagggtg taatcaatta tcggatctcg
     6601 atggaagagt ctttatcatc gatcctattg atggcacctt gaattttgtg attgaagggg
     6661 aaaatttttg tattatgctc gctgtttatg aatccggcgt tggaaagctt ggtttcattt
     6721 atgacgtgat gagaaatgaa cttttttggg gtggaaaagg aataggcgtg tataaaaatg
     6781 atgagccatt gcctagacca gcaaataaat ccttatcgga aggcttactt ggtgtgaata
     6841 gttatctttt cggtcataat aaatatggaa tccgagaaat tgcggaaagc agcatgggga
     6901 cacgtatgta cggatgcgct ggtcttgaac tagtggcaat cttgaaagga aaccacattg
     6961 cttatatttc aaaccttagc ccttgggatt atgcagcggg aaatgtctta ttggaagagt
     7021 ttggtatggt aacaagcggt ctttttggta ctcctttgac atttgaaaaa agggaatact
     7081 atttagcagg gacttcttgt gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct
     7141 gaaaaaaagg tagaaatcat tcacaaaaat gataaaaacg agcaaaagat taattttctc
     7201 gaaaaaatga aaacatttta ctatatttca tgaaaaaagt ctgctataat tagcagtcga
     7261 gtaaaatttg ccctgtcata aagaaccaat aaaaaaggag ttagttacat tgaattatag
     7321 aaacgatatc cgtaacgtcg caattattgc ccacgttgac cacggaaaaa caacattagt
     7381 tgatgaacta ttaaaacaat cagatacatt agacgcacat acgcaattgc aagaacgtgc
     7441 gatggactca aatgcgctag aaaaagaacg tggtatcacg attttagcca aaaacacagc
     7501 tgtagactat aaaggtattc gcgtaaacat catggatact ccaggacacg cggacttcgg
     7561 tggcgaagta gaacggatca tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta
     7621 tgaaggaaca atgcctcaaa cacgttttgt attgaaaaaa gcactggaac aacatatcac
     7681 accaatcgtt gtcgtaaaca agattgacaa accttctgct cgtcctgagc atgtcgtaga
     7741 tgaagttctt gaactattca tcgaattagg agcagatgat gatcagttag actttcctgt
     7801 gatttatgca tctgcactaa atggtacgtc aagcttgtca gatgatccag cagatcaaga
     7861 accaaccatg gcacctattt ttgatacaat tattgaaaaa atccctgctc ctgtcgataa
     7921 cagcgacgaa ccattacaat tccaagtttc attacttgac tacaacgatt atgtaggtcg
     7981 tatcggaatc ggacgtgtat ttagaggaac aatcaaagta ggagaccaag tagcattaat
     8041 taaattagac ggaacagtta agaaattccg tgtaacgaaa ttatttggtt tctttggact
     8101 aaaacgtcta gagattcaag aagcaaaagc tggagacttg attgctgtct caggtatgga
     8161 agatatcttt gtcggtgaaa cagttactcc tgtggatcat caagatgcgt tacctatcct
     8221 tcacattgac gaaccaacat tgcaaatgac tttcttagtt aataattcac catttgccgg
     8281 tcgagaaggt aaattcgtaa ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca
     8341 aacagatgta tccttacgag ttgaaccaac aaactctcca gatgcatgga cagtttctgg
     8401 tcgtggagaa ttgcatttgt ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt
     8461 gcaagtatct cgtccagaag ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt
     8521 tgaacgcgtg caaatcgata caccagaaga atatatgggt agtgttatcg agtccttgag
     8581 cttacgtaaa ggtgaaatgc aagatatggt ccatacagga aacggacaaa tccgtttgac
     8641 attcttaaca cctgcccgcg gtttgattgg ttattcaact gaattccttt caatgacacg
     8701 tggttacgga atcatgaacc atacttttga ccaatatctg ccaatgcttc ctggtcaaat
     8761 tggcggacgt caccaagggg cgcttgtttc gatcgataca ggaaaagcaa cgacttattc
     8821 tatcatgagt atcgaagaac gcggtacagt cttcgtagaa ccaggtacag aagtatatga
     8881 aggaatgatt atcggggaaa acagccgtga taatgatttg actgtcaaca tcacgaaagc
     8941 aaaacaaatg acaaacgtac ggtctgctac taaagaccaa acatctgtca tcaaaaaacc
     9001 aaaacaattg acgttggaag aatctttgga attcttgaat gatgatgaat attgcgaagt
     9061 aacaccagaa agcattcgct tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc
     9121 atctaaaaag aaaaaataat ctgtaaaagg atgaagagag aaaccagctt gttataaaaa
     9181 atagctggtt tctttttttg tccaactctt ttctgtggag aagtcatctt tgtcaatgga
     9241 cataaattca gaaaatacgc cgaaagacgt ttaaaagact tttaaaaaac ttatttctag
     9301 agagtttttg tttcataata agataatgtt tcacgaaagg agatcaactt gtccttcgaa
     9361 gaaaatcgaa gtaaaatggc aagagaaagg agcgggaaga tgaacgactt attaaaacaa
     9421 tactttggct atgatgagtt tagacctggt caaaaagaaa ttatccagaa agtcattgat
     9481 caggaaaatg tattaggaat catgcctacc ggaagtggga aatcaatctg ttaccagctt
     9541 ccagctttac tattagatgg gcttactgtg gttgtgtccc ctttaatctc tttgatgaag
     9601 gatcaggtgg acgcggcgaa tcaattaggt atacccgcca cttttatcaa tagctcgtta
     9661 gatggttacg aaacagctag acgcttccaa gaaatcgata gacagcagta ccgtttactt
     9721 tatatcgctc ctgaacgttt tatcatgccg gattttatcc aagcaatgaa gcgatggaac
     9781 gtccgtatga tcgctattga cgaggcacat tgtatctctc agtgggggca tgatttccgc
     9841 ccaagttatt tacagatggc gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt
     9901 gctttgacag ctacagcgac tgtgcaagta gctgctgaca taaaaagact tttaaaaatt
     9961 ccagaaaaca accatattca gactggtttt gaacgagaaa atctacgatt tcaagtaatt
    10021 aaagatcaaa aaaaagaaca atatctaatc gaatatttaa agatcaacaa aaatcaatct
    10081 ggtatcattt atgcagcaac gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa
    10141 ttcgattttt ccgtgggtag atatcatggt ggtctaaatg agaatgagcg aacagaaatg
    10201 caagaagcct ttttatacga tcgcttgcag ctgattgtag cgacaaatgc atttggtatg
    10261 ggtataaata aaagcaatgt ccgttttgtt atccattatc aaattcctgg ctctctagaa
    10321 gcatactatc aggaagctgg tcgggcggga agagatggtc tttccagtga agccatcctg
    10381 cttttctctc ctcaagatat ccaagtccaa aagtttttta tccagcagtc ccaaagagaa
    10441 gaagggcaaa agcaaaaaga atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt
    10501 gagtcctgtc ttcagcaata catcctaaac tattttggcg agacaagcag tccgtgcaac
    10561 cgttgtggta actgtttaga tgacagagaa atagtagaag taacgacaga agcacagatg
    10621 gttctttctt gcttaaaacg tatgggtgag aactatggta aacaaatgct gatgaaagtc
    10681 ttagcaggat cgaaagaaca aaagctgcaa gctttaggtt ttggacattt atctacctat
    10741 ggactgatga aaaaccaatc gcaaaaagaa acgatgcagt taattgaata tttgatatcg
    10801 aatggctatc ttctgaccat caatggtgag tatcctgttt tgaaagtaac agaaagaggg
    10861 atacaagtat tgaaaggaca agaatctgtt tatcgaaagg aacccaagaa agtccagcag
    10921 ctatcggatg aagaaaccga tacactattt gaagtgctgc gggaactgcg aacggatcta
    10981 gcatcagaag caggcgttcc cccgtatgtc gtattttcag attccacatt gaaagaaatg
    11041 agccgaatac gtccatcaag cagattggag atgcttcaga taaaaggagt tggccaaagc
    11101 aagcttgata aatatggcga agccttttta tctcggataa aaaatgcgga ctctaacgtt
    11161 ctggataagt agaaagaacg aatacgtaaa aatgaataaa aacaactggt taaatctgtt
    11221 ttttttgctc cttatgaaaa tcaaatatgt tataattaag gaattagaat gggaggtaag
    11281 cacagatgga ttctttgtcg aaaaaacttg cgatccaagt attaaatgat gaagcagaac
    11341 ggatcaaacg attaatccga aatcaaaaca acagtctctg tatctcacaa tgcaaagcct
    11401 tcgaagaagt ggtagataca cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc
    11461 gtataggagt aatcggaaag aacgagggcc agctgatgtt aagtgagcta gaacgagaat
    11521 tgaatcagtt atatagtgag gtttaccaag aaatttatga taaaaaagaa gttggcaagg
    11581 aggaatagac ttgcctaaga aagtgaaaaa aaggcatcta ttagactata gcatcttgat
    11641 tccctattta attttatgtg tattgggatt gatcatggta tatagttcta cgtcgtatct
    11701 attattagaa aacggacaaa atccgtccgc atcagtcatt aaccagagta ttttttgggt
    11761 gcttagcttg attgctatcg cactacttta taaaatgaaa acagatgttt tgaaaaacca
    11821 gcgtttgatc atggcagcca tcgcagtttt gacgattttg ctgctgattg ttgtgttttt
    11881 tggtaaggag atcaatgggg cgaaaggttg gctgcaaatt gcaggattct cgatccaacc
    11941 tgccgaatac ctaaaaatca tttctatctg gtacctgtct ctgactttat ctaaaagaca
    12001 aaacagtgta caaaaagatt ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg
    12061 acttactgca ttagtcgcta ttcttcccga ttttggtaat gcggcagtta tctttttgat
    12121 tattttggtt ctgcttttag ccagcggagt caattatgtg tatactttga ttgtaggagt
    12181 aggaggcttt tgtctaagta cttttacgat ctggctgatc aatatcacaa acggaaagat
    12241 cttacccgga cgtttacagt atatctataa tcgatttgct atttaccaaa atcctttctc
    12301 agacgaatta aataaagggc atcaactagt caacggctac tatgccatgt tcaacggagg
    12361 actatttggc aggggattag gtaacagtat ccagaaaaaa ggatttttac aagaagcaca
    12421 aaccgatttt atttatgcaa tcgtagtgga agaactggga gtgattatgg ggattttgat
    12481 attagcgttg ctattcttta tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga
    12541 tccgttcaat tcgcttttgt gcattgggat cggcgcaatg tttttgatcc aagtatttgt
    12601 caatcttgga ggaattactg gcgttattcc tttgacaggg atcacgttcc catttttaag
    12661 tcaaggaggc tccagtttgc tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag
    12721 tgcggatgaa aaacgcaaga gcttaggttt ataataaaaa aagatgtagg agtggttcga
    12781 tgaaaaaagt tttagttgct aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta
    12841 cagaattagg aattaaaact gttggaatct atgcagaaga agatgaatat tccgtacacc
    12901 gtttcaaagc ggatgaagca tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc
    12961 tagatattga agggatcatt tctatcgcga aagaatgcgg agctgacgct attcatccgg
    13021 gttacggtct tttgtcggaa aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca
    13081 tttttgttgg acccaaactg catcatttag atatttttgg agataaaatc aaagcaaaag
    13141 ctgcagctat cgaagcgggg attgcttcga ttccaggcac agatggacca atcgcttcta
    13201 tagacgatgc cttggagttt gcgaaacaat acggttatcc gattatgatc aaggcagctc
    13261 taggcggcgg cggacgcggc atgcgtgtag ctcacgatga aaagagtgca agagagggct
    13321 acgaacgtgc gaaaagcgaa gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa
    13381 aatacatcgc taatcctaag catatcgaag tacagattct cggggatacg catggaaacg
    13441 tcatccatct ttttgaaaga gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag
    13501 tagcgccttg tgtctctatg aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc
    13561 aattgatgaa atatgtcggg tatgtcaacg caggtacagt agaatttctt gttgaaggag
    13621 atgattttta ctttatcgaa gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa
    13681 tgattacaga tattgatatc gtaacaacgc aactgttgat tgctcaaggt ttggatctgc
    13741 ataaagaaat cggattgcct cagcaagaag gaattaaatt aaatggttca gccattcaat
    13801 gccgaatcac tacagaagac ccgttgaata actttttacc agacacaggg aaaatcgata
    13861 cgtatcgttc acctggtggt tttggcgtac gtctggatgt cggaaatgct tacgcgggct
    13921 atgtagtgac accgtatttt gattcattgc tagtaaaagt atgtacgcat ggtgctacct
    13981 ttgaaacagc cattcagaaa atggaacgtt gcttgaaaga atttagaatc cgtggagtga
    14041 aaacaaacat tccatttatg ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg
    14101 caaaaacaac cttcattgat agtacagcaa cattatttga atttcctcga ctacgagacc
    14161 gcggaaacaa aacgatgaaa tatatcggtg aaattactgt gaatggtttt ccaggtatcg
    14221 aaagtggcga aaaaccattt tatgaagaac cacgtatgcc taaagatcta atcacaagaa
    14281 ctgattacgt aacggctaag aatgtattag atgcgaaggg tgccgatgct ttggtcgaat
    14341 ggatcaaagg acaggaaaat ttgttgttga cggatacgac atttagagac gcccatcaaa
    14401 gtctgctagc aactcgtgtt cggacaaagg atttcaagca gattgcacgt ttgactggag
    14461 aaggtcttcc tgaactcttt tcaagtgaga tgtggggggg agcaacattt gatgtggctt
    14521 accgcttttt gaatgaagat ccttggcaac ggttgagaaa aattcgttct ttaatgccta
    14581 atacgttgct gcaaatgctt ttcagaggtt caaatgccgt tgggtattct aattatcctg
    14641 acaatgtatt ggtggaattt gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa
    14701 tctttgatag tttgaactgg actccacaaa tggaaaaaag tatccaagct gtacgggata
    14761 ctggtaaaat cgcagaggca gctatttgct atactggcga tatcaacgat ccaagtcggg
    14821 caaaatacaa tgttcagtat tataaagaca tggcaaagga actagaacaa ctgggtgcgc
    14881 atatcattgc aatcaaagat atggctgggc tgcttaaacc gcaggcagct tatcgtttga
    14941 ttagtgaatt gaaagaaacg acagatctgc caatccatct gcatactcat gataccagcg
    15001 ggaatggaat catcacttat tctgctgctt caaaagcagg agtagacatc gttgacgtgg
    15061 caatgagtgc aatgagtggg aatactagtc agcctagcat gagcagcttg tattatgcac
    15121 tggtcaatgg tccacggctg ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt
    15181 attgggaaga tgtgcgcatg tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa
    15241 cagaagtata tatgcatgaa atgccaggag gacagtattc gaatctacag caacaggcaa
    15301 aagctgtcgg attaggtcat cgctgggatg aaatcaaaca aatgtatcat actgtcaact
    15361 tgatgtttgg cgatatcgtt aaagtaactc catcatctaa agtagttgga gatatggcac
    15421 tatttatggt ccaaaatgat cttacagaag aagatattta tgaaaaaggc gaaacactaa
    15481 gtttccctga atctgttgtg actttcttcc aaggagaact agggcaaccc gttggcggat
    15541 ttcctaaaaa attgcaaaag attattctta aaggcagacc tgcattgaat gaacgtccag
    15601 gtcttttggc tgaatcagtc gacttcaacg aagtaaaaaa agaattggca gaaaaaattg
    15661 gctatgaacc aaaacaagac gaagtattaa gctatttgat gtatccgcaa gtctttttgg
    15721 actatcaaaa agcatataat caatttggcg atgttacatt actggacaca ccaacatttt
    15781 tccaaggtat acgtttgggt gaaacaatca atgtacaaat cgaacgcgga aaaacgctta
    15841 tcattcgttt agatgaaatc ggagaacccg atatcgaagg gaatcgcgta ctgttcttca
    15901 atttgaatgg acaaagacgt gaaatcacta tcaaagataa ttcgatcatc agtacggtcc
    15961 aaacccgatt gaaagctgaa ccgacaaacc gagaacaaat tggagcaacg atgtctggtt
    16021 ctgttttgga tgtattagtg aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga
    16081 ttactgaagc gatgaaaatg gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac
    16141 atgtatatgt gtcttcagga gacactattt cgtctggtga ccttttgatt gaagtgactg
    16201 agaaataata agcgaaaggt ggggaaacat gaaaagactg ggtttgtttt ggctgattct
    16261 gctattagtt attgtggcat tctacatgga accagtcttg tttccgccaa aacttgaagt
    16321 gaaggaaaac tctgatcaac aaatatcaaa ccaaatgaca acgtggaaat accaggagct
    16381 taaagcaact ggtttttcta cttatatcgg aaaaccgatc gatcagctgg aaaaggattt
    16441 tggccaagcc tatgatcgat taactagcgg atttggtttt gaaacaagat attatgaaaa
    16501 taaagctagt catctcacat ttgaagcgaa catccaagac agcagagtca caactgttaa
    16561 agtactgcaa acagggcctg ctgatatcgc gccgttttat ttagggatga cgatgcaaga
    16621 tcttacgaaa atcactacta tctatactaa tttcactttt gagtaccatg atacagaagt
    16681 tggaatcgaa ctaatggaag aggatatgaa ttatcgtccg ctaattgctt ttgataacga
    16741 cacttttgct attttatttt ttgatcaaac aacaaacggc ctattttcag ttgtgtattt
    16801 agataaagat agtcttttga aactattgcc atatcaagtg tttggtgaag gccttccaca
    16861 ttatcaagca gataaatcgg ctgattggga atcaatcaat cgcgttaaag aacgaaaaag
    16921 tacgacttta ttaaatatgc ttagaagaga agacgaactt cccatataca atcaaacact
    16981 tgcctttcaa aaccacagtc agctgctact acatgatttt ttgacaaggc ctgaggaaat
    17041 attgtcagag gaacgtatgg ctgattggca aaaatcgcta ggcagtgctc aaacgacagt
    17101 gaaattctca ctgacgaatg atgaattgga gaagttaatc agtaaacaaa aattagaaaa
    17161 tactagtggg ctatttattc atccggcgat tgacgctact ttttcgtttt tatattggta
    17221 cagcgatcct tacaaccatg accgtttcat ggacccggga acggattctg taggcattgc
    17281 tttttctaaa gaaaatatgt tagtcttatt gcaagaagaa aacggagaaa gtagtgatag
    17341 cagtgatcgt taacgaagag ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa
    17401 tgataaaaaa gagtaaaacg atggcttctt atcttgaaca taaacagtca atggaagaat
    17461 ctgcaaaagt ttgccagttg aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg
    17521 tagcggcata tggtgagtat gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag
    17581 caaaaagaca gcttgatatg gatgagcatg ttgcagcttt ccgatatagt gaaacacagc
    17641 tgcaaagtct gttggatcaa attgtccaag tacttgccga agaagtttct aatgaaatca
    17701 aagtagatgc aggtagcccg ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt
    17761 gtcatgcaat tagacaatag tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg
    17821 gtctacagct taaaacaatt aaagaatctg aaaaagttcg gcttgttgca ttatgtctca
    17881 agaagaatga aatatgtcgt gctttactta aatgaagaga cattggaaga aacagaagaa
    17941 cgaatccgta aattacattt tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa
    18001 atgaattttg ccgaaaaaat aggaaacaaa acagtattaa aagacgaagg tttcgaaatt
    18061 gaagagctaa atacagaaat ccgattagct gaacatcttt aaaaatctct tatgagcagt
    18121 ttttaaataa aaaataactt ggataacgat cgatttttga tcattgtcca agtatttttt
    18181 tggtatacta gtaccagtac catttctaag gagtgagtag atgcggataa tatcaggaaa
    18241 ttatggcggg cgccgtttaa aaagtttagc aggggcgaac accagaccaa ctacagataa
    18301 agtaaaagaa tccatattca atatgattgg accatatttt gatggagaga cagtattgga
    18361 tctgtttgca ggaagcggtg gtttagctat cgaagcagta tctagaggct gcagtcatgc
    18421 ggtatgcgta gacaaaaatt accaagcgct aaaaatcatc aaagaaaaca tcgaaattac
    18481 gaaagaacca gagaaattta ccacaataaa acttgatgcg gacaaagcaa tcgaacggct
    18541 agcagcggag aaacaacaat tcgactactt gtttcttgat ccgccatatg caaaacaaaa
    18601 aataatcgat caaatccata aaatggaaga actgggactt ttttctaaaa ccgcagttat
    18661 cgtatgcgaa acggataaaa cagtcgaatt gccagaaact gtggctggat tccatcaaat
    18721 aagaaaacag atttatggca taacggccgt aactatctat agaaaagagg gaaatcaatg
    18781 aaacgtgcat tattcccagg aagttttgat ccttttacaa aaggtcactt agatacggtg
    18841 gaacgtgcgg ctaaattatt cgatgaggta gtaattggtg tttttatcaa tacttcaaag
    18901 aaaagtttgt ttcccccaga agaaagaatg acattgatca caaaagctgt ttctcatctt
    18961 cctaatgtaa aagtcatgca tcaagaaaat caattgacgg tagaaacagc aaaagagatt
    19021 ggtgcagatg cgttgatcag aggcatacga agtatcaaag actttgaata tgaacgagag
    19081 attgctcaga tgaatcatca tctatatgat gaattggaaa ctgtcttctt attagcaaaa
    19141 ccagaatata gtcacgtgag ttccagtatt ttaaaagaag tactgcactt tggcggagat
    19201 gtgagcagct acttaccgcc agtgatcaac gaagctttag ctggaaagcg tgaatcaaat
    19261 gaaagctaaa caatggttga aaacagtact gattatcata tttggaatgt tgatcattgg
    19321 atttgtagta gttcccttgc cgtattatgt agaatcaccg ggagccacga tcgatctgaa
    19381 agaactaatt acggtcaatg ataaggaaga taaaggttct gggtctttct ctcttacctc
    19441 tgttggtatc aggcaagcta caggtttctt agctttaaaa gctaaaatga ccccttttga
    19501 agaattgatc agtgaagaag aattgactgg tggggcgaca aatgaagaat ataatcaaat
    19561 gcaacagtat tatatggatt cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc
    19621 agacaagcct tataaaatgg aattcaaagg cgtatatgtc atgagtgtag agaaaacatc
    19681 gaatttcttt ggcaagcttt cagttggaga tacagtaaca aaagtagacg ggaaaagctt
    19741 tcaaagtaca gaagcattta tggattatgt aaaaagccaa aaagttggac aaacaatcga
    19801 aatcagttat ttgcataatg gtgaagaaaa aacagcttct ggtgatctga tcgagttacc
    19861 aacagataaa aaggcaggaa taggtatcac gcttactgac cataccgaga tcgaatcaga
    19921 tacagatgtt cgtattaatt ctggctctat cggtgggcca tcagcgggat tgatgttcac
    19981 tttagaaatc tatgaacaag tcactggtaa aaacttgcga catggtaagc aaattgcagg
    20041 aactggaacg atcaacagtg aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt
    20101 ggcaagtgcg gataaagctg gcgtagagat tttctttgca ccagatgata cgatttcaga
    20161 cgatgttaag aaagaatatc ctgaaataaa aaccaactac gaagaagcaa aagctgccgc
    20221 taaaaaattg aattcctcaa tgaaaatcgt accagtaaaa acagtacaag aagcgcttga
    20281 ctatctagag aaaatgaaat gaaactaaat aattaaagag gggttgtgac acgagttctg
    20341 tcacaacccc tctttttcgt caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat
    20401 tgacgtaatt agatgttgaa gggacgattt tgccagagct cactgtttct tcaattttaa
    20461 atgaaaggag taatgaatga tttttactgg tcattctttg aaaaaagaac aatgatagaa
    20521 aaaatcagca agaaaatact agcttcaccc aaattgatca tttggatgtc tatgatagtt
    20581 cttatctttt gtatcgcaag tggtttttta ttatcaaatt tgctatttta tagagaaaca
    20641 gaagagactg ctgcaaaaga aatactttct tcgtttagtt ccagtggaga agatccatca
    20701 gctgaaggaa acgatgtaga agaggatgct cgtccaaagg tcatgtacac cgatatcaaa
    20761 ggaagtgtga aggagcctgg tatctattcc ttttcttcag aagaacgggt ctatgatgta
    20821 ctaaagcgtg caggtggatt attagaagaa gcggatagcg atcgaatcaa cttttctgcg
    20881 aagatagaag atcagcaagt cctatatatc cctgctgtag gtgaagagct gccagaacat
    20941 cttgatcagt tggcatcctc ggaaggaaaa cagcctacag cagattccga gcctcccaaa
    21001 ataaatatca atacagccac tccatcagaa cttcagcaga ttcctgggat cggatcagtc
    21061 aaagcgcaag aaatcattcg atttagagaa gaaaacggat ccttccagaa agtggaagac
    21121 ctgcaggaga tttctggtat cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca
    21181 attaaataga atgattcaaa atcgactggc acgagcaata aatggacgct aatggatact
    21241 aagaataatc caattctaag agcttggcag gacgaggagg tagtattttt ggataggaaa
    21301 tagcttgatt gttcgcaaag aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa
    21361 taaaatatct gggattcata ttatgaatac ggctgtcagc ggcaataaca gtcaagctct
    21421 acgagaacgc tttgccaaag atgtacttag ctatacagca gataaagtat ttattctcat
    21481 tgggacgaat gatctagcag agcataaaca actttctaaa gaaacgtacc aaaaaatctg
    21541 cagtggatga tagttcagct gaaaaaagac tatagttctg atcatgcttt ctttatcacg
    21601 ccaccagcag tcgatgagac aaaacaaaaa tatcggaata acaagttgat caaagaatat
    21661 atctctgttc tcacagaagt agcaaaacgc catgattgtg aagtatcgat ttttatacgg
    21721 aatttcttga acaaatggat tcgaagaatt tttataatgg gctaatggat gacggacttc
    21781 atttcggcag tgaagggtag gatcttttgt cagacaggat gattcgttat atttaaagaa
    21841 tattttgttt aagcaatgag tactttttaa tcaacttgaa aaaacggagg aatataaaac
    21901 atgttagaaa aatcttcaaa agcatgcgat acgaataaag aaaaagttac tgataagtca
    21961 cataatgaac gcattccttg ggaccagtac tttatggctc aggcagtact gttatcttta
    22021 cgtagtacgt gtacacgttt agaagtagga gcgacactcg ttcgagaaaa aaggatcatt
    22081 gctggcggct ataatggagc agtcagtgga gatgtccact gcatagatga agggtgttac
    22141 gtcgtggatg gacactgctt gcgaacgatc catgcagaga tgaatgcact cctacagtgt
    22201 gcaaaacttg gtatcccgac ggaaggttca gaaatctatg tcacacattt tccatgtttg
    22261 gcctgtacaa aggctttgtt gcaagctggt gtgaaaggga tcaattatct ccatgactat
    22321 cgaaatgatc cttatgccca agcgttgatc gaacaattag gtgtatctgt acaccaagtt
    22381 gctttagact cacagcattt ttcaaaactt caaaatgaat taacaggaaa acatgtctag
    22441 cgttttagaa aggcatgttc gaaatcggtt gatttttccg gcaattacgt cgatgattat
    22501 ctgtttgttg tattacactg acaattgggg ttatcgtttg cttcttgccg gaattttttt
    22561 atggttttcg ctaaaagaag gacgttttat cgcgtacttg tgtattttgt gtagcataac
    22621 ggtatccatt tcttataggc tacaagagta ttcattttta gaggaaaaaa caacatctgc
    22681 gcaaattact gtatttacag atacgattcg tgcaaatggt aattttttga caatggaagg
    22741 aaggttatca gagaataaac aaaagctcca gctttcttat caagtaaaca cagaagcaga
    22801 attgaaaaca tggcttaact tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt
    22861 catctctcca gcaaaaaagc ggaatcttta taattttgac caagaagctt acttcaggaa
    22921 ccatagaatt tctggacggt tccaaattca aaagattgaa cagatcacca tgcaaaatca
    22981 ctggcgaaac tggcttcaga gaaaaagagg atcttttatc tcctttgtcc aatctgaatt
    23041 tccgcaaaaa acaagtgtat atattaattc tttattatgg ggctataaag atgcaggatt
    23101 cagagaacca gaagaaattt ttagggagag tggtttgctg catctgttca gtctatcggg
    23161 tctacacatc caattttatt tagggtggct gtattattta tttcgacgta tcggatggcc
    23221 actacacgtc agtattattc cgttgagtct actaactatc tgttatgttt ctattgcagg
    23281 aagttctatt tctgttttgc gtgctagttt gctgttttta ttgcgattgc tagtcaaatt
    23341 gctgaataag cattattcca ccatggactt atttgcagtt gtcttttggc ttcttttact
    23401 atttgatcct cgatttcttt ttcatgcggg tggacagctt agttttttca tgagtttttt
    23461 gtttatcctg atttcttttg atggagattg gttgaaaaaa acggcctctc acgtcctgtt
    23521 tactatgatc acgatgccgt tgattgtatg gcatttttat gagtggccta ttttaggtag
    23581 tctttttaca ttactgattg caccggtatt caagtttctt tttcttcctg gtgtgttatt
    23641 tctcacactc ttcaatcgct ggctgccgga aactttactt ttgttgatag aagagggatt
    23701 aatcttattt gagcgaataa tcgatttttc ttcagggtcg tctctcatta taggacgttt
    23761 gccccttgtg ctatctggga tgtatatttt cggattactg atttgtatgg ataggttgaa
    23821 agaacaacct gtgaaacact tgttttatgt tggtcttttt tcttttattt ttttgacttt
    23881 gccattttta cgcttttctt caacgatcgc ttttatcgat gtcggtcaag gagacagtat
    23941 ctttttgcaa agtccttttc gaaaagaaac gattttgatc gatactggag gtcagttaca
    24001 ctttgaaaga gaaaagtggg cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt
    24061 tccatatcta aaaggtcagg ggataagtgt aatcgataaa ctcatcttga cacacgacga
    24121 tacggatcat gtgggagaat tacccacgtt atctcagcat tttactatca aaaaaattta
    24181 tatcggttgg ggcgctggac gaaatgagcc attacaaagg catttatctg aagctcaaaa
    24241 aaatggtact gagatcattg aaatcaaaca tggggacagg atcagcggtt attatgatct
    24301 ttatgtattg actccttttg aaaagggaga gggaagaaat gaagattcga ttggtctatg
    24361 gatggaatat aacaataggc gctttttatt tttaggagat ttgaatcaag caatggaaaa
    24421 acaattgctt ttgatttatc ctaatctgaa agccgatgta gtcaaattag gtcatcacgg
    24481 tagccgtacg tcaagtaaca cagattttct ttcccatatt gaagcaaaac atgggattat
    24541 atcctgcggc gtaaataatc gttatggtca cccgcacagc gaagtattgg agacactaga
    24601 agaaaatcag ataaaaacat ggcgaacgga tcaacaagga atgatcacct atcgctggca
    24661 tccagttttt catcctcatg ggctagtgga aacaatgata gactagtaat tctcatctgc
    24721 tcatggtagg ataatttttg aaagtggtga gtaatttgag tatacaagca gagttacaaa
    24781 aaatacgtaa agaaaaactt gcatcttgtt acttgatttt aggaacagaa aagtttctcc
    24841 aagatcaagt tcggacagaa attgtcaaaa aaatccaatt agagggcgaa gatgacttaa
    24901 attttttaag ttttgacatg gaaaatgcaa gtttagatga ggtagtagct gaagcagaga
    24961 cgttgccgtt tttcggagat caacggttag tattcgtaga gaatccttat tttttaacag
    25021 gtgaaaaagt aaacaatgga atcgaacaaa atactgattt attgacggct tatttgaaag
    25081 aaccattaga atcgactgta ttagtattct ttgcgccata tgaaaagttg gatgaacgga
    25141 aaaaagtaac gaaacaattg aaaaaaacag caattatcat caatgtccaa cagttgaatg
    25201 aaaaagaagt acgtcaatat ctgatgaata ccttagagaa ttcctcaatc aaaatggaac
    25261 gaagtgcaat tgatttattc ttgaggttga ccgacttaga tttatccaaa atgatgagag
    25321 agttacagaa attggtcctt tatggacaaa atcaacagca aatcacggta agagaagtag
    25381 aagaattagt tcctaaaacg ttagaacata atatttttga catgacccaa tatattttaa
    25441 aagggaagac ggaacaagct ttgcgtttat acgaagattt agttatgcaa ggggaagaaa
    25501 caatcaaaat taatgcgatt ttgctttctc aattacgttt atttttacag acaaaatttc
    25561 ttgtaaagat tggttatcag caggcaaata ttgctgaaac attgaaaatc catccatatc
    25621 gtgtaaaact tgccatgcag gaagtaaaaa aattcgatga acatttgctg gtacagttgt
    25681 ttgatcaatt agttgagatg gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa
    25741 gttttcaact attcgttttg cgcacagggc agttgttaag aaatcgataa gaagaaagac
    25801 gagggcaaag catgcgggaa ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat
    25861 cggccgaagc cgattctttt atttagcgat ttttttgctt aaacgagatt tgtcgcggtt
    25921 agctttgttt ttgtggatta gaccttttga ttcagccata tcaatagctt taacagcttc
    25981 tttatataac gcatctacgt tgtcagcacc tgaagctaca gcatcttcaa attttttgat
    26041 tgcagtacgc atagcgcttg tttgagatga atttttcaca ttagcgtttt cgcttgtacg
    26101 aacacgtttg attgcagatt caatgtttgg cattagtttc acctccgata acttaagttc
    26161 ttacgcgtgt taccgcataa ttcaacattc attattatac ctaaaggcag cgtgctttgc
    26221 aatatgaaca tgacaagttt ttttccttaa cagtctttta ttaggatttt ttttcagtaa
    26281 aaacggatag tacaagctga aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc
    26341 aaaccaaact agatttttat ttatctattt aaacaaagtt tgaatgggaa tgtttggtac
    26401 taagttatta tataacaaag agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt
    26461 ccctgttgtt ctttgttttt ctattaagaa actgcagtta ttttataacc agtatccttt
    26521 gatcagtaaa ggctattctg gtgtaccgat tattttaatg tattcatcga tattttttat
    26581 caacattttt tttatgacca actggttccg acggttccat ttgccactta cttccagcac
    26641 atatttattt aggggcaaac gtaagatata ttttgccaaa tagcgatctt taagcaggcc
    26701 attgatcaca cggtcctcaa gctgcaagtt gatcccagtc agcatcattg gctgttcttg
    26761 taaaacgtac gtatcaatta aaagaccttc aaaagtttcc acaagcgtct tcctttcttt
    26821 tcaaagtaac ctgaaaacag gcactaattc ttccattttg atattcgttg aagtggatgc
    26881 gatcattctt tcttttgtcc actgtaatct agtataacaa aaaagagcag aagagccaaa
    26941 aagaaaaaag agcatcgaat tctataggaa gtctcattgg cacggcctaa attattacaa
    27001 aacattgatt aaaaaaagcc cctgcatcgc aggagcatcc cctatatcgt tttaataggt
    27061 caatcaaaaa gcagtatcaa acacattttg tatcaaatca ggaaataata gataagctaa
    27121 gcccagaatc aaaagtaccg ctagaagaaa ccagccgatt gttcctatga aatactttaa
    27181 tttttcgaac attgtatcac ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt
    27241 taagaatctc ctaaaataga caaaatttgt tctattttcc taaagcaaat acttgtaaga
    27301 tacagtaagt gataccagaa gcaatgaccg gtccggcggc gattcctctt aagaagacaa
    27361 cacctaaaat cgtgccgaaa acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc
    27421 ctacaccctt ggaagataag acagccacta gcccaccgca aaaaacagct acccagccaa
    27481 taggggattt aaaggcttct aatagatcac gtagaccgat ttctcctgtt gcgataggga
    27541 ttagaatagc cgctgtgatg atcgtcaccc caatattgat tcctttgcct tgaagccaag
    27601 ggaaaaagga acgggaggct ggtatgagct tcataatcaa aacaaaagct gccgctatca
    27661 tcagactttg atttttagct aaaattgcta caagtaaaat aattcctaaa aaaagccaac
    27721 tttccatgaa atatattcct cactttcttc gaaacaatca taacatgttt ttagaaagtt
    27781 acaaaagtag gaattcatcc ttcaagacct attcataagc tgtttttttc ataaaataaa
    27841 caaaatcata aaaaaacact ttgcttattt gacagaagac agccgcgctt tgtatagtgg
    27901 acaagactta catttttttg aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa
    27961 gccattcaga ataaccaata ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt
    28021 taaaaattgc aggaagttag aagcaatcaa caagagaata acaagaatcg aaaaaataat
    28081 attttgtttg atgatccgcc gcatttttaa agatagccga tgactgtaga caaatttatt
    28141 taagtcgctt ttcattaaga cgatgtctgc gatatccata gcggcatctg tcccttgccc
    28201 catagcgaca cctaaagaag cggctgctaa agcaggcgca tcattgactc catcaccaac
    28261 catcgcattt acttggtaaa gttcattctc ttttttgact agttgtgttt tttcttccgg
    28321 agtacagctt gcatagtaat gttcaatccc tacttgttca gcaatggcct ttgctgtgcc
    28381 agaatggtcg ccggttatca tacttgtatg gatgttttgg gatttaaagt aggaaattac
    28441 ttgttgggta tttgcttttg gaatatctag cagacctaaa acagcaatta actgctgatc
    28501 tttagaaaga taaatcacgg ttttgccttg attttccaaa cgttcgatca tttccgcaat
    28561 ctctttgctg attatcatcg tctcaggatc atatgcgtgc ttgccaactt tccattgcgc
    28621 acctaaatag gtagtttgta atccaaaacc agtaatttct tctattggta agtattggat
    28681 ttcttcaggt attgtgcaat cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt
    28741 tttttgttcc atagcaacaa gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct
    28801 gtcagttaca actggttttc ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt
    28861 taattcagaa agcttttcta aatggatacc gcctttgatt aaaatcccat tttttgcaga
    28921 atttgaaaga gcagctagag aagctggtgt agcagatgcg acaagcgcac aaggagaagc
    28981 cacaactaat aataccattc ctcgataaaa gctttcatcc caactccaac ctaggaaaaa
    29041 gtgggggaga aggatcatta gaggaacagc aagcaaaatc aattttacat aattattttc
    29101 gattcgttcg ataaatgaag ctgtttgagt tggcgtattc tgcgcttctt ctactaattg
    29161 gataatctta gcaaagacag tttcggaact cgtttttgtt actcgaatcg tgatgggatt
    29221 tccttgattc agcgttcctg catacatctc atcgcctaaa tatttttcta caggcacaga
    29281 ttcaccgctt aatgcagact catcgatcgt tgcctgtgtg ctttctaaaa agccatctat
    29341 cggaacagct gctcctttag cgacaaatac caagttatcc acctttagtt gagaaacatc
    29401 tacttcttgc atttgtccat tttcttttaa taattgggct ttttgtggtt gaagattcat
    29461 caaagcagta atttcttttt gacttttatt agttgtgtat tcctctaatg caccgcttaa
    29521 acaaaagatg aatgtcaaca ttgctccttc aaagtaatta ccgatcaaac aggcaccaat
    29581 agcagctaaa gccatcaata aatccacatt cagtgtccga tcattccaca attctttcaa
    29641 gccttccgat gtttgtttat atccaccgat gataatggca ctgatataaa aaagtggggt
    29701 gatggtaaga gagaatcggt cgaagataaa accgaagatc ataaataaaa ggcagccgat
    29761 tgttgctgca aaagcttttt tctgattagt catcgttatt agacctcctt gtcaaaacaa
    29821 ggatagcata ataaaaatgt aattgataat aataacagct aaatgagaac gataatggtt
    29881 atcatttggt gattaagctt gatgttgttt ttattattta taatgattat tattaaagaa
    29941 aataaaaaac cagaacattt ctcaaataag agaagtgttc tggttttttc tacattgcaa
    30001 taaaccaata accaatcaaa atgataccaa ggataatacg ataccagcca aaagcggtaa
    30061 agtcgttttt cttcaaatag cttagtaaga atttgatagc taagacggaa accacaaaag
    30121 atacgaatgt tcctacaaga agaacaacag tttcttccat tttgaatgaa ttcccttcag
    30181 ctaaatattt gacgatcttc aagccgcttg cgccaaacat gacaggaatc cctaagaaaa
    30241 atgaaaactc tgtagcgaca aaacgcgaag caccaatcaa gatagatcct aggatcgttg
    30301 cgccagaacg ggatgttccg ggaacaagtg ccaagacttg gaataaccca acaagaagag
    30361 ctgcttggta cgtaaagtca ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt
    30421 tttcgacaat aataaaggca ataccgtaaa cgatcaaaac aatggcaact ggtaagaact
    30481 tgtggaaatt tgcttctaac caatcatcca aaggcaaacc aatgatgatg gctggtatgc
    30541 aagcaacgat cactttaaac caaagtgtcc acgtatcttt tttttctacc gttgatttac
    30601 ttggagaaaa tgggtttaac ttgtgaaaat aaagaacgac gacagccaag atcgccccta
    30661 attggataac gacattgaac atcgacataa attgtgaact ggcatccagt ttgatgaatt
    30721 catctgctag gatcaaatga ccagtactac tgatgggcag ccattccgtt attccttcaa
    30781 taataccgag aatcactgct ttgataatat ttgcaaaaaa cataaattcc ttccttccca
    30841 attaagatac atcttatagt ataccgttat tttttgagaa acccaatctg ttttttaata
    30901 ttaagaaaaa cttgagaaat aaagcagata agtacacgaa gagaaaaaag aagacaaagc
    30961 cctcgttcag actttgcctt ttatgattct ctggtaaaaa ttaaaatatc cccattatag
    31021 aaacgagtcg cgactatgtg ttttcccgtt atcgatataa gacgtatcat tgatgccgtg
    31081 gatagaaaat cgaccgtttg cataagtgac ctttgttacg ctgccattta aaagcccagc
    31141 acgaacaggc tgtttaggat cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga
    31201 tacgactaaa acatttccac caccattttt ttcgcagtga tgggcgatat cttcaaatcc
    31261 tgtccataca cggtctcgca atgtttcata agtttccgcc cacccagcgg tatctaagtt
    31321 gtatacagca ttagcaattt gttcaaaaga aacttctgtg gtaaacattt catcatatgt
    31381 tttaaaattc aatacacgag ggatcacacc ccacatttcc atgtcatatc caccttcaag
    31441 agacccgaaa caccattctc taatcctttt ttcaataaaa taaggaattt cttttccctt
    31501 agaatgttct cctagaataa tacgggcagt ttcgattgca cgaccactgt cactagaata
    31561 agcaaatgaa aagtcgacat tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc
    31621 ttgctttgtg aggggtgtat cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt
    31681 ttttccgtgt cgtacgatat aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt
    31741 tttaacgatt gaaaaaagga tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat
    31801 gtcctggata gactgcaaat tcatcaggta atacaaacag ctgggtccga atactatgaa
    31861 gtaattgttc taaattccct gtatacagat cggttctacc gatacttcct ctaaacaggg
    31921 cgtcgccaga aacgacaaaa tcatcaaaaa taaagctgac gcttcctata gaatgccctg
    31981 gggtagggac gacgcgaaaa gacatgtttc ctaaagtata agttttcatt tcaaattcat
    32041 attctgcagg cgagacaata acatcagcaa tatcatcatg tctaccgagt ccagaaagat
    32101 tcaaaatcgg atcacctagc catttttgct ctagtgggct gacataaact gggatttgat
    32161 aatacgcgcg taaaggttcc actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa
    32221 tcgctaccgg ctgttggttt gtttttttga tttcttgttt gattcgttcc gcatcttctc
    32281 cagggtcaat gatcaaaagt gcttcttcgt tgtagaccaa ataacagttt tcttcaatca
    32341 ttcctgtttt taatctttcg atatgcatgt gcattcctcc ttgatagcca aaacttcttt
    32401 catccattag tataacgcat tcattgctag gaaacaaaaa caggtactta tttctcttta
    32461 cttatctgaa ttatcagact atatgaaaag tatttggata ttctaaagag aaggaaagta
    32521 agttttcata gcattttaat atgtccttgt tacattagat tcatccaaaa atccaaattc
    32581 tatatcctat atccctgcct aatcagcagg gtgttttttt atgtttggaa agagagctct
    32641 ttttgctaaa ctaggttttt tcaggtaatc ttagagagaa agaggtggtg gaaatgatca
    32701 aacagtcaaa aatatctgtg cggcacttag tagaatttat tttgcgacgt ggaagtatcg
    32761 acaatcgtaa aaaaagtaac catactgcat tagaaggagc gagaatccac cgcaagctcc
    32821 aaaaagatgc gggagaaaac tatgaaaaag aagtcttttt aaaaacaaca gttcaattag
    32881 atgaatatta cctgatcgtt gaaggccgtg cagacggtat tttcaaaaga gacggtattt
    32941 attatataga tgaaatcaaa acctccgaac cacgatttga agatttagaa ccagaacaag
    33001 tggaactttt ctttcatcaa gcaagggtgt atgcctatat ctatagtcaa gaacatgatt
    33061 tacaagaaat aaatctgcaa ttgacttact tccagacggc agaagaagtg atcacgagaa
    33121 aggttgaaca tcagacaaga gagcagttag aaactttttt taaaaagctg actgaagatt
    33181 ataaagaatg gcttgttttt caagaaaact ggcgcactgt acgcaatgcc tctttgatgg
    33241 ctttgaaatt cccccatgat gagtatagga aaggtcagcg tgaactagcg gtagctgctt
    33301 ataaaacgat ccgtacgaaa cagaaattgt ttgtagaagc acctaccgga acaggaaaaa
    33361 caatctctac gttattcccc gctttgaaag ctgtagggga agaagaggga gaaaagatct
    33421 tttatctaac ggctaaaaca ataactagac aagtagctga agatgctatg acagcattaa
    33481 aagatacagg agctgaagta aaaagtgtca ctttgacggc gaaagacaag atctgctttt
    33541 tagatgagac gacctgtaat ccagatcagt gcccatatgc caatggttat tacaatcgga
    33601 ttaatgaagg tttatgggat cttttgaatc atgagaatca gatcactcgt gaagtcatcg
    33661 aaacttatgc aagaaagcat acactttgtc ctttcgaact atcattagat gtcagtatct
    33721 ggtgtgacgt aatcattggg gattacaatt atttatttga tccaactgtt tatttaagac
    33781 gtttttttga agatgaaaaa aacgaggact atctgttttt gattgacgaa gcacataatt
    33841 tggtgaatcg ttcccgagag atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa
    33901 gtaaggaagc tataccaaag gaattcaaaa aacttcatcg ccgtttcaac aaactattaa
    33961 aagaatttga ttcgatccga gaaatagcaa aagaagatca ttgggattat catcatcaaa
    34021 aagcaccggc tgaatcttta gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat
    34081 ggctggcaga atttcctgag cacccatcac aagaacaatt gcttccttat tattttgatt
    34141 tgcttcattt tttgaaggtc agtgaatttt atgatgatca ctacgaaaca acagtcgaaa
    34201 aaacgtatcg cgatctgata gtaaaagagt tttgtattga tccaagcttg tttcttgagc
    34261 agtcgttgga taaaggaaaa agcagtattc ttttttctgc tagtttttca ccactctctt
    34321 attatcagga aacactgggg ggacaaaaaa gtctggctta caaactgccg agtcctttcc
    34381 cagaaggaaa ccagcaagtt ttcatcgcaa attacctaga gacgacttat cgaaaaaggg
    34441 aacacagtct ggcaaaatta gtagagacga ttcatgcttt tgctgatgga aaggtcggga
    34501 attatttcgt tttctttcct tcttatcaat atttagatct tgcggtggaa gcattcagac
    34561 agcaatatcc cgacagcaat gtgctaatcc aagaaacgga catgaatgag gaagaacgcg
    34621 aacgattttt aacaaaattt caaacaaatc ccgctgaaac attgattgga ttttgcgtac
    34681 ttggcgggat tttttcagaa ggaatcgatt tgagagggga ccgattgatc ggcagtatga
    34741 tcgtaggtgt aggtttgccg cagatgaatc atgagcagga actgatcaaa tcatattatg
    34801 atgagaagga acaccttgga tttgcttacg cttatcagct tcctggaatg aacaaagtct
    34861 tgcaagctgg tggtcgagtg attcgtggca tggcggatca aggaatcgtt gtattggcag
    34921 atcaacgatt ttctagtttt tcttatcgac gtttattccc acaacattgg caaacagcaa
    34981 gagaagtacg aagtgttggg gaattggaag aaggaataca gcgtttttgg ttatcaaaag
    35041 aagcagtaaa tcaaaaaaac gaggaatgac agacgtcagc ctcgtttttt gattaacgat
    35101 agttagtgaa ttgcaactcg attggtaagt caagtttacg taataaagca atcacagctt
    35161 gtagatcatc acggttctta cctgtcactc taatctgatc ttcttggatt tgtgctttta
    35221 ctttcagttt ttcatttttg atagctgtag tgattttctt cgcgttttca cgatcgattc
    35281 cgttgaccaa atcggcagtt tgtcgagctt tgccacctag agcgtgttct gattcggaaa
    35341 aatggatatt tttgattggc acatttcttt tgatcagctt accaaataat acatctttga
    35401 tctgttcgat tttgaaatca tcatcgctca atacaactag attgctgttc tctaatttga
    35461 tttctactgt agaatccttg aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa
    35521 tcgcattttt tacttcttct gtattgactt ctgatacaat gtcgaagcta gcatcttttg
    35581 ccataggaac cctcctgata tacatataaa tatttaccat acatctatct tatcatagga
    35641 ttagggcaga ttcttacctg tttgctaaaa ttcttctcat ttgagttcga agtattttat
    35701 tttaatagat aaactgttat tcttacattt ggaataccaa agtaggggag aggtgcttgt
    35761 gagtaaaatt atgactgcgt atcataaaaa tgaaagatta aagaaaatag tggttatttt
    35821 gataaccgga ttgaccgctg cggtcgcttt aaattatttt ttgattccag ccaatgtctt
    35881 ttctgcaggg atgaatggga tcgctcaaat catttcctcg cttttatatg aatggtttca
    35941 tatcaagata gacacaggaa catttatttt tcttttgaat atccctgtgt ttgtattagg
    36001 gtttctcaaa gtaggaaaac gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc
    36061 ggtgatgacg atgctgcttc cagtcggaca gatcactgat aatattttga tgaatgccct
    36121 agtaggcggt gttttattag gtcttggcgt aggattatct ttaaaaatgg gctttacaac
    36181 tggtgggatg gatatcatct ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa
    36241 ctacatgctg attctaaatg gtatcattgt agtcatcgca ggcttcttct ttaactggga
    36301 aagtgccttg tatacgatta tttccattta tgcaatgact caagtaattg atgccatcca
    36361 cacgagtcat caaaaagtaa cagcaatgat cgttaccgtc aagccagaag aggtaacaga
    36421 agaactagca aaacggatgg tacgtggaat gacattactg ccgtctgtcg gtggctattc
    36481 aaaaaaacaa gggaacatga tcatgatggt catcacccgc tacgaaatgt atgatcttga
    36541 tcagatcgtt catgaaatcg acgacgatgc atttatcaat attttaccaa ctcattcagt
    36601 atttggccgg tttgcaaatg aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc
    36661 agaaataaaa aatcgttcac aaaaaaaatg aggaatcagg tttattttag aataaattta
    36721 atctattaca tctaataaga acatttttat aaatttaaac gagaatctta cttctaaatt
    36781 ctcatttaca agcagctaaa aaaagagtat gataaataaa aatcgtgtta aggagtgtcg
    36841 tacgatgagc gaagcaaacc aagaacggtc tggacaagaa atagaactaa tcaaagaacg
    36901 tattcttgct gccttggaaa tggtgattga tcctgaatta ggaatcgaca tcgtcaattt
    36961 aggcttgatt tatgatatag aattcaatcc tgaaaatgga gaaacagtca ttaagatgac
    37021 tctaacaact atgggctgtc cattagcaga tattttaaca gaatctatcc atgacgcatt
    37081 aaaagaagtg ccagaagtaa cgaaagcaga agtcaaacta gtctggtatc cagcatggac
    37141 gacagataag atgagccgtt atgcacggat tgcgttgggg atacgataga aatcttggct
    37201 gactagctat gagaagcaaa taggaaaaac aaaaaagcct tttatccttt ctagaagaaa
    37261 agagataaaa ggtttctttg tttataaaat tttgtctgct atattataag ttaattaaat
    37321 aaatgtgttc tattgataaa ggacattcaa atccctttcc ttttggaatc cttctttttt
    37381 acatcagttt gattttcgta tagtattctt tctcatcttc acgataaatc gcagccccat
    37441 ttttttgttg tgctttaaga ctgccaagat tttttttatt tacagacaaa agtgcctcat
    37501 caatgccgat tttttttgcc tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt
    37561 ttttacgata attaggggag atgccgtaac caatatggcc agcgccgttt cttaatgttt
    37621 cgttcagttt atggcgcaaa ttaaaaattc ctacatactc atgttcatca ctttctaaaa
    37681 taaagacggt tgcgggaacg taactctcag gaagatcgat tcctaaagac gttttttcgc
    37741 atttttttac ataatctaaa aattcagcaa atgtatagcc ataagcattg ttgataaatc
    37801 cactctcatt tggtccaaag ctagtttgta gctgataagc tttctttaaa tctgcttccc
    37861 atagtttgat cagtttcatc gtattcacct ctttaagtaa ccttattcat ttgttttcta
    37921 ctatagcact attgttgtaa atagccatgc ctatttttat ttattgtgtc atatcgtatt
    37981 aagaatcatc gaacaataca aatatgaatt acaataaaaa aattatttga agttcgttca
    38041 ttcgttactt atgatctcta gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc
    38101 agaaggatag taatcaacag aagaaataat caatatccaa atgaaagaag atagctgaat
    38161 cgagaaaaag aggaaaaaag aaatgaagaa aaaaataaaa tagttactaa tcccagagtc
    38221 gttcgtttaa cattgaaatc gatcatatgg ataaaacaga ttttttcacc aaccctcaat
    38281 agatgatgga gttgctcgga tgaattattt gtgtagatgg cataagggag ggcgtacaga
    38341 aatgaaaaag aacaaaaata tgttcttgac aaaaaacaca tgtaatccta aactaaaaga
    38401 tgtgatgcgg ctatggcgga attggcagac gcgctagctt caggcgctag tgagggtgac
    38461 ctcgtggaag ttcgagtctt cttagccgca taatttgttt ttttataaag agaaggtaca
    38521 cctggagcga tgagaaagaa aataacatat tatctaaaag aatataggaa aaattttaaa
    38581 caacggaacc agattcaaat aaatcaaaaa aatttgtgtg aaggtgggac agtttaaaac
    38641 ctaatagatt gattcaaata gtagatttat agattctgat acaagaaata ctggcaaacg
    38701 atagttaagg aataaagact aaacttaaac tcaaaaagag gtcatgcgaa agaataaatc
    38761 gcatgacctc ttactatatt ttgaatgtgt ttaaacgttc aaataatgaa tcttagtacc
    38821 aaccgt
//
LOCUS       DACCSK010000013        35715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCSK010000013 DACCSK010000000
VERSION     DACCSK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.013"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            118..1065
                     /locus_tag="IVP39_000589"
     CDS             118..1065
                     /locus_tag="IVP39_000589"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7585686.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1085..1612
                     /locus_tag="IVP39_000590"
     CDS             1085..1612
                     /locus_tag="IVP39_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7585687.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1675..2328)
                     /locus_tag="IVP39_000591"
     CDS             complement(1675..2328)
                     /locus_tag="IVP39_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7585688.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2460..3302
                     /locus_tag="IVP39_000592"
     CDS             2460..3302
                     /locus_tag="IVP39_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7585689.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3460..4356
                     /locus_tag="IVP39_000593"
     CDS             3460..4356
                     /locus_tag="IVP39_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7585690.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            4359..5072
                     /locus_tag="IVP39_000594"
     CDS             4359..5072
                     /locus_tag="IVP39_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7585691.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            5092..5610
                     /gene="msrA"
                     /locus_tag="IVP39_000595"
     CDS             5092..5610
                     /gene="msrA"
                     /locus_tag="IVP39_000595"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7585692.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            5607..5834
                     /locus_tag="IVP39_000596"
     CDS             5607..5834
                     /locus_tag="IVP39_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7585693.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            5986..6537
                     /gene="lepB"
                     /locus_tag="IVP39_000597"
     CDS             5986..6537
                     /gene="lepB"
                     /locus_tag="IVP39_000597"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7585694.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(6620..7162)
                     /locus_tag="IVP39_000598"
     CDS             complement(6620..7162)
                     /locus_tag="IVP39_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7585695.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(7399..7797)
                     /locus_tag="IVP39_000599"
     CDS             complement(7399..7797)
                     /locus_tag="IVP39_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7585696.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            8057..8917
                     /gene="ylqF"
                     /locus_tag="IVP39_000600"
     CDS             8057..8917
                     /gene="ylqF"
                     /locus_tag="IVP39_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7585697.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            8910..9677
                     /locus_tag="IVP39_000601"
     CDS             8910..9677
                     /locus_tag="IVP39_000601"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7585698.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            9733..10590
                     /gene="dprA"
                     /locus_tag="IVP39_000602"
     CDS             9733..10590
                     /gene="dprA"
                     /locus_tag="IVP39_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7585699.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            10712..12790
                     /gene="topA"
                     /locus_tag="IVP39_000603"
     CDS             10712..12790
                     /gene="topA"
                     /locus_tag="IVP39_000603"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7585700.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            12759..14135
                     /gene="trmFO"
                     /locus_tag="IVP39_000604"
     CDS             12759..14135
                     /gene="trmFO"
                     /locus_tag="IVP39_000604"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7585701.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            14333..15238
                     /gene="xerC"
                     /locus_tag="IVP39_000605"
     CDS             14333..15238
                     /gene="xerC"
                     /locus_tag="IVP39_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7585702.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            15275..15823
                     /gene="hslV"
                     /locus_tag="IVP39_000606"
     CDS             15275..15823
                     /gene="hslV"
                     /locus_tag="IVP39_000606"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7585703.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            15837..17237
                     /gene="hslU"
                     /locus_tag="IVP39_000607"
     CDS             15837..17237
                     /gene="hslU"
                     /locus_tag="IVP39_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7585704.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            17255..18046
                     /gene="codY"
                     /locus_tag="IVP39_000608"
     CDS             17255..18046
                     /gene="codY"
                     /locus_tag="IVP39_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7585705.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            18128..19003
                     /locus_tag="IVP39_000609"
     CDS             18128..19003
                     /locus_tag="IVP39_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7585706.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(19135..19773)
                     /gene="plsY"
                     /locus_tag="IVP39_000610"
     CDS             complement(19135..19773)
                     /gene="plsY"
                     /locus_tag="IVP39_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7585707.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            19900..20334
                     /locus_tag="IVP39_000611"
     CDS             19900..20334
                     /locus_tag="IVP39_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7585708.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            20500..22545
                     /gene="parE"
                     /locus_tag="IVP39_000612"
     CDS             20500..22545
                     /gene="parE"
                     /locus_tag="IVP39_000612"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7585709.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            22557..25007
                     /gene="parC"
                     /locus_tag="IVP39_000613"
     CDS             22557..25007
                     /gene="parC"
                     /locus_tag="IVP39_000613"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7585710.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKKYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            25284..27515
                     /gene="pflB"
                     /locus_tag="IVP39_000614"
     CDS             25284..27515
                     /gene="pflB"
                     /locus_tag="IVP39_000614"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7585711.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            27759..28520
                     /gene="pflA"
                     /locus_tag="IVP39_000615"
     CDS             27759..28520
                     /gene="pflA"
                     /locus_tag="IVP39_000615"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7585712.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            28605..29531
                     /locus_tag="IVP39_000616"
     CDS             28605..29531
                     /locus_tag="IVP39_000616"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7585713.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            29713..30606
                     /locus_tag="IVP39_000617"
     CDS             29713..30606
                     /locus_tag="IVP39_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7585714.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            30740..30865
                     /locus_tag="IVP39_000618"
     CDS             30740..30865
                     /locus_tag="IVP39_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7585715.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(30999..31631)
                     /locus_tag="IVP39_000619"
     CDS             complement(30999..31631)
                     /locus_tag="IVP39_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585716.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(31779..32321)
                     /locus_tag="IVP39_000620"
     CDS             complement(31779..32321)
                     /locus_tag="IVP39_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7585717.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            32456..32845
                     /locus_tag="IVP39_000621"
     CDS             32456..32845
                     /locus_tag="IVP39_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585718.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            32849..33613
                     /locus_tag="IVP39_000622"
     CDS             32849..33613
                     /locus_tag="IVP39_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7585719.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            33742..34689
                     /locus_tag="IVP39_000623"
     CDS             33742..34689
                     /locus_tag="IVP39_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7585720.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            34819..35559
                     /locus_tag="IVP39_000624"
     CDS             34819..35559
                     /locus_tag="IVP39_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585721.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT        12546 a         6160 c         7453 g         9556 t
ORIGIN      
        1 tttcactcga ttacaagaat tgcaaaacga cgtatctgaa acagaagcac aacttgaaga
       61 aaaaatggca cggtgggaat acttgagtga atgggtagaa gattaggagg aaaaaggatg
      121 gaacaagcgt atcttgactt aggaagaaaa ttgttggaag aagggcatcg taaagaagac
      181 agaacaggca caggcacatt aagcttattc ggataccaaa tgcggtttga tctgcaaaaa
      241 gggtttcctt tgttaacaac gaaacgagta ccttttggat tgatcaaaag cgaattgctt
      301 tggtttttaa aaggagatac gaatatccgc tatctattac aaaataaaaa ccatatttgg
      361 gatgaatggg catttgaacg ctacatcaaa agtccagact ataatggacc agatatgact
      421 gattttggcc atagatgttt gacagatcct gaattcaatg aagaatataa aaagcagagc
      481 aagctttttt gcgagaagat cctaaccgat gattcctttg ctgaaacata cggcgatctt
      541 ggacacatct acggctatca atggcgtcac tgggaaacaa aagacggtgg ctttatcgat
      601 caaatcaaac aagtgatcga agcaatcaaa aagactcctg attctagaag attgatcgtt
      661 tctgcatgga atccggaaga tgttcctagc atggctttgc ctccttgtca tacgatgttt
      721 caattttatg ttcaagaggg acggctaagc tgccaattgt atcaacgcag tggggatgtg
      781 tttctaggcg ttccgttcaa cattgcaagt tatgcgttgc tgactcacct aattgcacat
      841 gaaacaggtt tagcggttgg cgaatttgtt cacacattag gagatgctca tctttatatc
      901 aatcatatcg aacaaatgaa agaacaattg agccgagaaa tacgcgcatt tccgacttta
      961 gttttgaatc aggaaaagca gtcagtattt gattttgaga tggaagatat ccaattagaa
     1021 ggatatgacc cacatccggc gatcaaagct cctatcgctg tttagaatca gaaaggatga
     1081 gaaaatgtta gctgcaattt gggcacagga tgaaaacgga ttgattggaa aagaagaccg
     1141 tcttccttgg cgtttgccaa atgatctgaa attcttcaaa caaatgacag aagcaaatac
     1201 actagtgatg ggacgaaaaa cattcgaagg gatgggtagc cgtccgttgc cgaatcgtca
     1261 gacgattgtt ttgacgagag attcttccta taaagcagaa ggcgtgcatg tcatgcatag
     1321 tgtggaagac gtgcttgctc tggaaaaaga aacagacggt atctttttca tcgctggagg
     1381 gtcagctgtt tatcaagaat ttctgccatt ttgtacgata ttataccgta cagtcatcca
     1441 tcatgtattc gatggagatg cttattttcc acctgttgac tggtctgatt ggtcactgat
     1501 caatactagt caaggagaaa tggatgaaaa aaatcgttat ccgcatcaat ttgaaacgta
     1561 tcagcgtaat gagaatcctg taaaaagcgg gacaggcgaa ttgacgatat aaaaaagcta
     1621 tggatagact agctgttatt tagagctgtc ccatccatag ctttattttt tttattaagt
     1681 aaagaataaa acagagaagt acataaaacc tgcacctaac ataacaaaca aatgccaaac
     1741 aacatgcata tagcggatag aattcaagct ataaaagaat gcgcctatcg tgtaagaaag
     1801 cccgcctgct accatcaaaa taacaccagt cattccaaga gagtgataaa gaggaacagc
     1861 agccacgaca cagagccacc ccataaaaat atagatgatc gtagatactt ttgatactct
     1921 ctcccgttta tgcaacgtca acgctttata tataatgcca agaatcgcaa acaaccagat
     1981 taatacaaat aaagtccaac ctagccatcc tcggatactc agcaaacaaa aaggagtata
     2041 acttcctgca atcaaaagaa aaatcgaact atgatcgaat acttggaaca ccttttttgc
     2101 ttttgtaaaa atcaatgaat gaaacaaagt agacgttaaa aataaaagaa tcatcatcga
     2161 tccatagata gcataagaga caatatggag ggaggaatgc aagtgggcac cttttactaa
     2221 aagaaccact agtcccgcga tactcaatcc agtcccaatt ccatgggtga ctgcattaaa
     2281 cacttcattt aatatttcat acgttcgatt aaatttcgtt ttttccatga aaacggctcc
     2341 tttactaggt gtaattaatg aaaaatcatt atttgtctgt tatactaaaa atagaaattg
     2401 tcttacatgt agtttaacat gaataaggca tcttgttaaa gaaagagaga gagaaaataa
     2461 tgacaaatgt aaaaatcgta acagattctt catgtacgat ggaaaaaaat gtacgcgatg
     2521 ctttaggcat tcgtgtcatg ccgctatcca tcatgatcga cggaacagta tatcccgatg
     2581 atgaccaact agacggcgaa agatttatgg aaatgatggc gaaagcaaaa aatctgccca
     2641 aaacaagcca accaccaatt ggagaattca tctcgatgta tgatgagcta ggagaagacg
     2701 gcagtgaagt gatttcgatc catatgacaa aaggcttgag tggaacagta gaagcagcaa
     2761 gacaagccag taatctaaca aaaacgaaag taacggttat cgacagtgat accactgacc
     2821 aaggattatc ttttcaggta attcgagcag ctgaattagc aaaagaaggt aaaacagtag
     2881 agaaaatcct tgaagaagtc aaaaaagtac aagacaatac aaaattatac atcggaatat
     2941 ccacactaga taacttggta aaaggtggaa gaatcagccg aacaactggt ttgctgtcta
     3001 gtgttttgaa catacgcgtg gtgatgaatt tcgcccattc tgaattaatc cctgtaacta
     3061 aggggcgagg aaagaaaacg ttcgataaat ggtttaataa tttgaaaaat gaactgaaag
     3121 aattgccaaa tgtgcgacaa atcggtattt cacatgcgga tgcaaaagaa ttggcgcaag
     3181 aattcaaaga gggtttgcaa gaattattcc cggacatgca tatccctgta ctgcatacga
     3241 atccggttat cgctacgcat actggtaaag acgcgtttgc aatcatgtat tatgtagatt
     3301 agtcgaaaag aggataagcc ctcagcctga aaggcttgca gcttattctc tttttcttta
     3361 taaaattagg aaaataattg ttttcttttt tcataaagac tacctttttg ataaaatttt
     3421 tgataacatt agaaccaatc gaatacaaga ggtgaaaaaa tgaaattaac gaaacgacat
     3481 ttgatcctcg ttttgttccc attattaatt gcgatcatca tctatgcagg tttagctgct
     3541 tctataccga aagctggccc actaatgaaa ccagtaacta ctgtgagaga agatccaaat
     3601 aaaaaagaaa aacttcacta catagctatc ggagattcgc tgactgaagg agtaggagat
     3661 caaaccaagc aaggaggatt cgttcccctt gtcgcaaatg atctaaaaga ccgttataat
     3721 ttgaccgcgg ttgaaacaga aaactatggt gtaaatggtg aacgaagtga ccagatctta
     3781 aagcgagtaa aaacaaaaga agaaattcga aacaatatca aaacagctga ttttatcaca
     3841 ctgactgttg gcggtaatga tttgatgaaa gttatccaaa ataatttatt cgggttaacg
     3901 gtcaattcct ttaaaaagcc actgaaaaac tatcaggaaa atgtcacaga actattggaa
     3961 gaatttcgat ccctcaataa aaccgcacct atttacattt taggcatcta caatccattt
     4021 tatttgaact ttcctgagat cactgacatg cagacaattg ttaacaattg gaacaaagga
     4081 acagaaacgg tgacaaaaaa aatgccgaac tgctacttta tcccaatcaa tgatttgctt
     4141 tatcaaggat tgaatcagga agtaggtgtc acagaagaag gaacagatca aacaactagc
     4201 acgacagaaa atctaaatgg catcaaaaat aatgtgctct atgaagaaga tcatttccat
     4261 ccaaataatt taggttatca attgatggca aatgcagtga gagatcagct gatcgagaca
     4321 caaaaccaat ggttagagaa ggaggatgat caataaacat gaaaacaaga tctgacgtat
     4381 cttcaaacaa aacaaagaaa acaaattact ggaaattcgc cttccttatc cttcttgggg
     4441 tagtacttgg ttcgaccgta tttttaggca gccgtatttt tgctaatcgg gaaccagaac
     4501 tcccagaaat accagctctt acggaaagac aaggagatcc tgtcttaacg atcaattcaa
     4561 ataaagaaaa agtcaatcaa atcatcagct ttttccttag tgagtatcaa aaagatagtg
     4621 atattgaata taaattttat ttggaaaatg aagcactact caatggtact ttcgaagtac
     4681 taggttttcc tatcgatttc tatttatatt ttgatccgta tgtgatggaa aacggaaatg
     4741 tccaactaaa agctagaagt ctttctatcg gaacattgag tttgccaatc aaagatgtga
     4801 tgaatatgat caagagaaac tataaattac cagattggat cgaagtcaat acagatgatc
     4861 tgactatcat gcttaggttg gatcaattcc gaatgcaaaa cgggatgtat attaaagcag
     4921 ataaaatcga cttagtaaat gatgacattc ggtttagtct atatttgcca aaagatacct
     4981 ctgatgcttc aaacaactca aataccaaca aaactagcac cagcagtact gaaagcaaga
     5041 caaaagaaaa taaaaaatca tcggatgagt agtattagag ggggaatcat aatggaacga
     5101 gcaatatttg caggaggctg cttctggtgt atgatccagc ctttcgacac gcttcctggt
     5161 atccatacag tgatgtcagg atatactgga gggcatgtac cagatccgac ttatgaacaa
     5221 gtaaaaagcc aaacgactgg acatacagaa gcagtggaga ttatctatga tccaacattg
     5281 atttcatatg aacaattagt agaactttac tggcagcaaa ctgatccaac tgacgctttc
     5341 ggacaattcg aggatcgcgg tgacaattat cgcccggtga tcttttattt gacagaagaa
     5401 cagcggatca ttgctgaaaa aagtaagaaa cagttagctg aaagtggacg tttcgaacag
     5461 ccaatcgtta caaaaatcga accagcccaa ccattttatc tagcagaaga agaccatcaa
     5521 gagtattata aaaagcatcc tgaaaatttt aaaagaaacc atgcacgccg ggctgccttt
     5581 atcgaagaaa attggaggaa cgaagggtga atcgaagctt ttttcattat ttaatgacac
     5641 tgcgaggcgg tggcacacat gatgctttat ctgttttcgc tacagaagcc ggtaaagaca
     5701 tccaatttcc taagcacagc tctagctacg aagaaatttc taattatctg gaactgaatg
     5761 tcgactattt gccgtccatg gatatctttg acgaagcatg ggaaaaatat ttagaaaata
     5821 atcatgacat ataagagata gttttgtact ttatgatcaa aaaagttttt tgtccaatag
     5881 cctaattgaa agaattattt ttatttcagc tatctagtcg catttttttg atatttttct
     5941 tataataaag aaagaatgaa aaaaagaggg atggattgat agagattgac aaaaaaacaa
     6001 cgctatatta atcgcttctg gcttgtattc aaatacttac ttgtttctgt attccttgcg
     6061 ttcatgttgc ggggattttt attcatcccg gtaccagtgg aaggcaattc aatggaaaac
     6121 gtgttgaagc aaggtgatat ggttgtgatg gaaaaatttt cggagatcag acgatttgat
     6181 attgttgttt ttcaattagc agatggcacg atttatatca aacgagtaat cggtcttcca
     6241 ggagaaaatg tcagctacca aaatgatcaa ttgaagatca atgggaaagt agttaaggaa
     6301 ccttatctaa ctaaaaacct gaaatcagat catgcaaatg catcctatac gacggacttt
     6361 acactacaag agctaacggg acaatccaag cttccggaag ataattattt tgtacttgga
     6421 gataatcgaa gagtttcaaa agatagtcgt tcgtttggca cgatcaataa aactgatatt
     6481 ctaggtaaag caagatttgt ctattatcca cttgatgaaa taaaatggat ccagtgagaa
     6541 acaataaagc gtgcagtctg ttgagactgc acgctttatt gtttaatcca aaatcagttt
     6601 actaccttag tacgtaaaat caaacagctc gtttaaacac ttttctagcc tctaaagttt
     6661 tgataaatga ataaacaacc attatttgaa tagggatttg gatcatattc ctgatgaatc
     6721 tacctagaaa aattactttg aaatctgcac caaacaaaac agacatcagc caagaattga
     6781 taaaaaggct gactgggatc ataatcaata attgggcagc gatgacggaa ggcaggttta
     6841 tctttttatg atataaaaat aagccattaa taaatccacc aaggaaagcc gtgataagaa
     6901 aagcagcttg aaacgttcct gaaccaaata tcgtgaaatt taaagtgttc gacaacattt
     6961 caagcaaacc agcccagatc ggtccatata gataacccat aagcgccata cttaaaaatc
     7021 cgaatgtgat ttgaaaataa gcaggaccaa tcgctatctt gtataagatg acatcaaggg
     7081 ctaataaaag agcagaggtc gacaacaatc ttgcattcat ttcgggcaat ctaaaaacgt
     7141 cttttgcaat caaagtgttc aaaatattct ctcctttgtc agttaataat aaaaggatac
     7201 cataaaatca ccaatgtgtc atgacacatt ggtgatttac atacactggc atctaacaat
     7261 taaaaaaagg gtgagaagac tgcttttata aactgtaaaa aagagattgt acgaataaaa
     7321 aaaatagagg cggagaatac ttatcgtatc atccgccttt gtttttttgt tataaaaaac
     7381 gtcagattag ttatctgctt acatcttcat ccatacgggt tctaatgtat ttccgtctag
     7441 atcttggact tctaatgaaa acatttcttc ttcaggcatt cccatatcta cttcgtggaa
     7501 agtaccacca ttttcttttg caatttgagc aaattttttt acttcatccg cattttcaac
     7561 actaaacgaa atcaaagcac cacttgtttt tttattatca gctatttctt tatcttttat
     7621 aaatttttta taaaaatcat gagagagaag cattatccaa aaagtgtcat cccataccat
     7681 tgaaccagct tcttcagtag aaaattcttc atttttttta aaacccagct tttcataaaa
     7741 agcaacggaa cgattgacat cagcaacagg aaaattaaca aaaaccatac tattcatttg
     7801 tttcgtctcc tttctgtttt gttcgtctat catctcttat ttaggaaacg ctgtcaaaaa
     7861 tttagattca tatatagaaa ccaaaataaa aatttgcatc atcaaaaaag tgaagatgtg
     7921 atagaatgag gactggttca ctttcactta actaatagat ggaatttctt tataataaaa
     7981 aagtgaattt atattgaaaa agaaaaaaac aagtaaagcc ggggaatatt ccggtatgaa
     8041 aggaatggag agaacaatga ccatccaatg gtttccggga catatggcaa aagcccgacg
     8101 agaagtttct gaaaaaatga aatatgtgga catcgtattt gaattggtag acgcacgatt
     8161 acctttatct tctcgtaatc caatgctaga tcagatcatt cagcaaaaac ctcgccttgt
     8221 tttattgaat aaagcggatc tggcagatcc acaacagaca aagctgtggc aagaatattt
     8281 tcgtaaacaa ggccatcttg cattagctat cacggcacaa gaaagtaaag gaattaaagg
     8341 tctagtacct gcggctaaag aagcattgaa agaaaaacgg gcaagagatg ctgctcgggg
     8401 gatcaagcct cgcgctattc gggcaatggt cttaggtatt ccaaatgttg ggaaatctac
     8461 gttgatgaat cgactagtag gcaagaaaat tgcacagaca ggaaataagc ctggcgtaac
     8521 aaaaggtcag cagtggcttc gtttaggtaa tgagttggaa ttgttagaca caccagggat
     8581 tttatggccc aaatttgaag atgaagaaat cggcaaaaaa ctagcgctga caggcgcgat
     8641 caaagaccag cttgtccatt tagatgattt agcgatttac ggattagact tttttgcccg
     8701 atattattca ggaagaatca gcgatcgtta tggtttatca atcgagcaag aagaaaaatt
     8761 ggcaccggaa ttactgatgg atattacagc aaaacgtggg tataaagaag actatgatcg
     8821 tggcagcgaa ttagtcgtgc atgatattcg tcaaggaaaa cttggacgat acacactgga
     8881 tcgttgcgaa gaaatggagc aaaaaagtga tgactgaatc catccaaagt atcaaagaac
     8941 gattgaaaag catacagaga atagatgatc ctacactaga atcattaaga aatgatccac
     9001 gcaaaggcgt gcagcaagca ctggcttctt ttgaacgacg attgaaaaag gaagcattaa
     9061 ttaaggaaaa atatgaagaa atgttttgct ttgaaaagca agggtacgca caaggtcacc
     9121 aatggattgc cggcattgat gaagtaggaa gaggaccatt agctggacct gtcgtggcag
     9181 ctgcagttat cttgccgaaa aatgagatca tattagggct gaatgactcc aaacaattat
     9241 cagagaaaaa gcgagaactt ctttatcaac aagttcaaga aaaagccctg gcaatcggga
     9301 tcgggatcgt tgatcagcag acaatcgatg atgtgaatat ttatgaagca agtaaattag
     9361 cgatgatcca agcagtagaa aaattgtcca ttgtaccaag ctatttgttg atcgatgcga
     9421 tgaccttgga tttgcctatt cagcaggaaa aaataatcaa aggggatgca cgatctgtgt
     9481 cgattgctgc tgcaagtatc attgctaaag tatatcgaga ccaattgatg aaagaatatg
     9541 acgtgcttta tcctggatat ggtttctcgc ataatgcagg ttatggaaca aaggaacact
     9601 tagaaggact tgaacgacaa ggaattacgc caatccatcg taaaagtttt gcacctgtaa
     9661 aaaaatattt acaataaaaa acagccgctt ttacgtatct tattagaaag agagacgaaa
     9721 ggcggtttta tgatgtatca aatagaagaa aatttattga aacttgctta tacaaaagga
     9781 atcagtagtc aaggaaaatg ggcacttgct aactggatga tgcagtatcc ttataaagaa
     9841 atcggatttg aagacatcat taagataggt aaaataacca aacaccggga gctgtttcgc
     9901 aaaagttggc aggaaattca cgctaactgg ggcaaaatcc aatcgaaaca gtcgtttatc
     9961 acttgctttg atcccaaata tccccctcag ctgcttcact tgacctatcc gccaattgta
    10021 ctgttttata atggtgatct atctttactt tcttgtaaca tgttaagcgt tgttggtgga
    10081 agacacactt ctgctttagc caaaaaaaca gtttattatt tactaaaacc agtgatagaa
    10141 gctggatatg tgatcgtcag tggctgcgcg aaagggatag atacatacgg tcaccaagca
    10201 gcaatctctc atggtggacg gacaatcgca gtcattggaa cagggataga gcaagtctat
    10261 cctgctgaaa atcgtgactt gcaagaggtg attgcaaatg atcatcttct tttgagtgaa
    10321 tatcctcctc aatccggccc gaaaaagcat tgttttccaa tgagaaatcg gattatcgca
    10381 gctttgtcta aaggaacatg tgtctttgaa gccaagaaaa aaagcggctc tctgataaca
    10441 gcacagcaag cattggatct tggaagaaca gttttttctg ttccaggaaa tattttaaca
    10501 gaacattcta ccggatgtca tcatttgatc caagacggtg cagtttgcac ggtttcgggt
    10561 gaagatatct tggcagaatt taaagaataa caccatactt ttcatttgct tttcttgaca
    10621 aacgttttct gaatggatat gattagctac gatttgtatg aatcctcttt taatagtagg
    10681 aacacgacaa tggaaaggag tccattcagt aatggcgtat aaatatttag tgattgtaga
    10741 atcacctgca aaagccaaga caattgagaa atatcttgga cgaaactata aagtaatggc
    10801 cagtgttggc catataagag atttaccgaa aagtaaaatg ggtatcgatt ttgaaaacaa
    10861 ttatgaaccc cattatattt ctatacgcgg aaaaggcgat gtcatcaaaa gcctgaaagc
    10921 cgcagcaaaa aaagctcaaa aagtttactt ggcaagtgac ccggatagag aaggagaagc
    10981 gattgcttgg catttagcgt acctacttgg gttggatctg aaagaaaaaa atcgggtggt
    11041 cttcaatgaa atcacaaaag acgcagtcaa agcagctttt aaggaaccaa gaacgatcga
    11101 tgtagattta gtagatgcac agcaagctcg tcgtacctta gacagaatcg ttggttattc
    11161 gatcagtcct attctctggc gtaaggtcaa gaaagggtta agtgcaggac gtgtccaatc
    11221 tgtcgcttta aaaattatta ttgaccgtga aaaagagatc cgagaatttg ttccagaaga
    11281 atattggagc atcgacggta attttaaaaa agctcgcaag aaattcaaag caaatttctg
    11341 gggaatcgac ggtaagaaaa agaaattacc agatgcacaa agtgtaaaag aagtcactgc
    11401 tagaattgat ggaacagact tcgaagttaa aaaagtagaa aagaaagagc gcaaaagaaa
    11461 ccctgcgaat cctttcacta caagtagcct gcaacaagaa gctgcccgta agttaaactt
    11521 tcgaacaaga aaaaccatga tggtagcaca gcaattatat gaaggaatca ctttgggtaa
    11581 acaaggaaca gtcggcttga ttacatacat gcgtactgat tctacccgga ttgccgatac
    11641 agcaaaagca gaggccgcag catttattga agatacgtat ggaaaagaat tttctagaca
    11701 tgatcaccga aagatcaaaa atgctcaagg cgcacaagat gcccatgaag ctgtgcgtcc
    11761 aacgagtgtg cttcgtacac cagatgaact caaacaatat ttagataaag atcaattgaa
    11821 actttataca ttgatctggt cacgttttgt ggctagtcag atgacaccag ctattttaga
    11881 tacaatgaaa gtgacattag aacaaaatgg tgtcatcttc attgcgaacg gttcaaaaat
    11941 caaattcaaa ggattcatgc aagtgtatgt ggaaggtaga gatgacggca aagaagagaa
    12001 agaaaatatt cttccagaac ttgaagaagg cgatgtagtc caatctgttg atattgaacc
    12061 aaaacaacat tttactcaac caccagcacg ttttagtgag gctacgttga ttcgtgcact
    12121 agaagaaaac ggggtagggc gaccttccac ttatgcacca acacttgata cgatccaaag
    12181 acgctactat gtaaaattga cacaaaaaag atttgagccg actgaactag gagaaattgt
    12241 caattcatta atctgtgaat tcttccctca aatcgtcgat atccatttca cagcagaaat
    12301 ggaaggagat ttggataaaa ttgaagaagg cacggaagcg tgggtcaaag tggttgatcg
    12361 tttctataaa ccgtttgaaa aagaactgac gaatgcggaa gaaaaaatcg aaaaaattca
    12421 aatcaaagat gaaccagcag gattcgattg tgatgtctgt ggacatccga tggtgataaa
    12481 attagggaaa tatgggaagt tttatgcttg cagtaatttt cctgattgtc ggaatacgaa
    12541 accaatcgta aaagaaatcg gtgtgacttg cccagtatgt catgaaggac aagtcattga
    12601 acgaaaatct aagaaaaaca gactattcta tggttgttcc cgtttcccag aatgtgaatt
    12661 cacttcttgg gataaaccag tgggcagaaa ttgtccaaaa tgtgacggat atctggtcga
    12721 gaagaaagtc aaaggcggta aacaagtagt ctgtgtaaat ggagattacg aagaagacat
    12781 ccaaaaataa tagaaaagag gttctttcat tgaaaatggt aaatgttgtt ggagcagggc
    12841 ttgccggaag tgaagctgct tggcagatcg cacaagcagg agtccctgtc cgtttatatg
    12901 aaatgcgtcc agtcaaatcg actgaggcgc atcacacaag taattttgct gaactagtat
    12961 gttccaattc attaagagga aacagtttgg ctaatgcagt agggttattg aaagaagaaa
    13021 tgcgccgatt gaattcggta gtaatcactt cggcagatga aacagcagtg ccagctggtg
    13081 gtgccttagc tgttgaccgc gattcttttt ctgaaacaat tacccgaaaa gtcaaagagc
    13141 atccattagt aacagtgata aacgaagaaa ttactgaaat accggaagga atcaccgtag
    13201 tagcaacagg acctttgact tctcatccat tagcagaaag catcaaagca ttcaatggtt
    13261 cagatggttt ttatttctat gatgcagctg caccaatcgt agacaaagct acgatcgata
    13321 tggataaagt atatttgaag tcccgctatg acaaaggaga agcagcttac ttgaactgtc
    13381 cgatgacaga agaggaattc aaagctttcc atgaggcact aatcaatgcc gaagtggcag
    13441 aacttaaatc ttttgagaaa gaaaaatatt ttgaaggatg tatgccaatt gaagtaatgg
    13501 cacagcgagg attcaaaacg atgcttttcg gacctatgaa accagttgga ttggaagatc
    13561 ctaaaactgg caaacgtcct tatgctgtta tccagctgcg tcaagacaat gctgcagctt
    13621 ctttatacaa catcgtgggc tttcagactc acttgaaatg gggcgagcaa aaacgtgttt
    13681 tccggatgat tcctgggtta gaaaatgctg aattcgttcg ctatggtgtg atgcaccgaa
    13741 acagctttat gaattcgcca gaattgctga aaccgactta ccaatcaacg aaacgcgaag
    13801 atctgttctt tgcaggtcag atgactggtg tagaaggcta tgttgaaagt gcagcgagcg
    13861 gactacttgc tggaatcaac gccgcacgct tagcaaaaga aatggaacca gtcgagtttc
    13921 cacaagaaac agctattgga agtatggctt actacattac tcatgcggaa ggaaaacatt
    13981 tccaaccgat gaatgccaac tatggtctgt ttcctgaact gccagagcgc attcgagaca
    14041 aaaaagcaag atatgaagca attgcacaac gagctttaaa agctaatgag gcaataaaaa
    14101 cagaattaga aaaaactgca atcccaaatg agtaaggaaa ctccatgaag agggtgagac
    14161 ggatagtctc gctctttttc ttgcgttttt cttaagaatt tctcaaacaa aatgtgagga
    14221 attgcgccaa ttgtgaacaa acttagaaat attggttaaa ttctgacaat ttatttgtct
    14281 ttttataaac gctgtgctaa attattctct gggacaaaag gaaggtgaga aaatgacaga
    14341 agccacgtgg gaacaaaaat ttttgcgtta tttgattgtc gaaagaggat attcagaaaa
    14401 aacgaaagaa gcatatgaag aagacattct ccatttcaaa cattttttgg aggaatctgg
    14461 agacgctgat ctgctgaagg cggaatattt tacggttcgt gtctatttga gtgcattagc
    14521 tgatcatcgt cctagctata gcagaaattc tatcagtcgc aagatagcca gcctacgttc
    14581 gttttatcag tatcttctca aagaagaagt gataaaagaa aatccatttt cttacgttca
    14641 tctcaaaaag aaaaatttga gactgccacg ttttttttat gaaaatgaaa tgcaagcact
    14701 ttttgacagc gtagcaggag atagtccttt ggatttgagg aaccgcgctt tactggaagt
    14761 cctttacggg tcaggcatcc gtttaagtga gtgtagcagt ttgtcggtat cagacattga
    14821 ttttgattca gaggtcatgt tgatccatgg taaagggaac aaagagagat atgctcctct
    14881 aggttctttt gctcaagatg ctctgcaaga atatttcagg gaaggccgcc aggtattgat
    14941 gacaaaatat cacgaagagc atgatatcgt ttttgtgaat catcatggaa aaccaatcac
    15001 gcctactgga attgaatatg tcttgaatca aatgatcaaa aaaagcagtt tggatagtga
    15061 tatccatcct cacatgctgc gccatacatt tgctactcat ttattaaata atggggcaga
    15121 tttgcggaca gtccaagaat tgctaggaca tgccaattta tcgactaccc agatctatgc
    15181 ccatgtaaca aaagaaagtt tacaaaaaaa ctatcgttct ttccatccaa gagcgtaaaa
    15241 ataaggaaaa gaacaaattt aggaggaaac aaacatggtc gaatcaccat ttcattcaac
    15301 cactatctgt gccgtcgaaa aagacgggaa atttgctatg gctggagatg gccaagtaac
    15361 aatgggtgaa tctgttgtca tgaaaggcac tgcaaagaaa gtacgacgta tctacaatgg
    15421 agaagttgtt gtaggtttcg ccggaagtgt tgcagatgcg tttactttag aagaaaaatt
    15481 tgaaggcaaa ctaaacgaat ataacgggaa tctacaacgc gcagctgtcg aattagctca
    15541 agaatggcgg acacagcaat ctatgcaaaa acttgaagcg atgctgatcg taatgaatga
    15601 taaagaaatg cttcttgttt caggtacagg agaagttatc acaccagatg atggtatctt
    15661 agctatcgga tcaggaggga attttgcgtt ggctgcagcc cgagcaatga aaaactatgc
    15721 tcaaaacgac atgagtgcga aagaaattgc agaaaatgcg ttaaatattg ccgcagatat
    15781 ctgtatcttt actaatcata atattatcgt agaagaactg taagaggtga gtagatatgt
    15841 atgaattaaa acaaacacca agacaaatcg taagtgaatt agatgaatat atcgtaggac
    15901 agcacgcagc aaaaaaatct gttgccgttg ctttgcgtaa ccgctatcgc cgtttgcagt
    15961 tagatgaaaa aatgcaacaa gatattacac caaaaaatat gctgatgatc ggacctacag
    16021 gggtcggtaa aacagaaatc gcacgacgtt tggcaaaaat cgtcaatgca ccatttgtca
    16081 aagtcgaagc aacaaaattt actgaagtcg gttatgtcgg aagagatgta gaatcaatgg
    16141 ttcgggattt agtagaaaat gcgatccaaa tcgtagaaaa agagcagtac agccgtgtat
    16201 acacaagtgc taaaaaaaat gcagaaaaac gtttggtaaa aatcttggta cctggtatca
    16261 aaaaagaaca gaaacaatcc actaatcaat ttgaacaaat gatgaatctt tttaatgcta
    16321 gtcagcaaaa taccgctgaa ccgcaagaag aagtgacaga agatattcga atcaatcgcc
    16381 gtacagtctt ggaacaacta caaaagggtt tactggatag tcgtgaagta accattgaag
    16441 ttgaagacca aaagaaacct gcaccaatga tgaacaatgg attggagcaa atggggatcg
    16501 acctaaatga aactctaggt gctttgacac cgaagaaaaa agtggaacgg acggtaacag
    16561 tcaaagaagc tttagagtta ttgatcaagg aagaatcagc aaaattagtc aaggatgctg
    16621 atatccatag tgaggcaatc aaattagcgg aagaaagtgg aattattttt atcgatgaaa
    16681 tcgataagat cacttctaaa tcacagcaat ctggagaagt atccagagaa ggcgtccaaa
    16741 gagacatatt gccaattgtc gaaggttcac aagtaaacac aaaatacggg atcatccaaa
    16801 cagatcacgt cctattcatt gcttcaggag ctttccattt gtctaaacca agtgatctaa
    16861 ttccagaact tcaaggacgc ttcccgatac gggtagaatt agatgatttg acagctgaag
    16921 attttgtgaa gattctgacc gaaccaaata atgctttagt aaaacaatac gtcgcgttga
    16981 tcggtacaga aaatgtgtca gttacgttta caaaagaagc aattgaacgg atcgctcaaa
    17041 tcgctttcga tgtcaaccgt gacacagata atatcggcgc acgcagactg cataccattt
    17101 tggaaaaact actagaagat ctattatttg aagcaccaga tatgcaaatg ggagaaataa
    17161 cgatcacaga agcatatgtg gatgacaaac ttggctcaat tgcggcaaat gaagatttaa
    17221 gtcgctatat tttataaaaa atcggaggac gacaatgaca gacttgctaa cgaaaacccg
    17281 caagataaac acacttttgc agcaaaagaa tacttttgac atgcaagcag atttgcctta
    17341 cgataaaatg gctgtcacac taggtggtat tcttgacagc aacacctata tcataggaag
    17401 tgacggagta ttactaggtt ataatgaacg tcacgatgtg aacaatgaac gaatcaaaac
    17461 aatgtttgtt gagaaacaat ttcctgaatc ttatactgat acagtggcac agttgatgaa
    17521 aactgaagct aatatcccaa ttactagtga tattactgca tttccggtag agtcaagaga
    17581 gaaatatcca tttggtctga cgactgtcgt tccgatcttt ggagctggcg aacgtttggg
    17641 tacgattatc ttatctcgta tggaacaatc gtttgcagat gacgatctag tcttagcaga
    17701 atatggagct acggttgtcg gtatgcagat cctttatcag aagtctcgaa gcatcgaagc
    17761 agatgtacgg agtgcaacgg ctgtccaaat ggcaatcaac acattatctt atagcgaatt
    17821 gaaggctgta caagctatct ttgaagcatt ggatggagat gaaggtcgtc tgactgcttc
    17881 taatattgcc gatagtatcg gcatcactcg ttctgtgatc gtcaatgctt tacgaaaatt
    17941 ggaatcagct ggaatcattg aatctcgttc gttaggcatg aagggaactt atttaaaagt
    18001 attgaattcc cgttttaaag acgagttagc aaaacacagt tattagaagt aaggggactg
    18061 gggcagatag aaatgtttgt tccagccttc ccattttaaa aagtaagtcg aagttggagg
    18121 aatggcaatg actgttatta ttcaaaatga ccagctgatc gccgaaatcg ctgaacacgg
    18181 tgcggaattg atcagtctga aatcaaaaga acatggttta gagtatatct ggcaaggtga
    18241 tccagcgtat tggggcagac atgcaccggt tttattccct tttgttggac gtttaaaaga
    18301 tgatcagtat gtttatcaag ggaaaacata tcaaatgggg caacacggat ttgctcggga
    18361 tatggatttt gaagtcattg aacatggaca agaaatggct agtttcatct taaggagtac
    18421 gccggagaca ttagaaaagt atccatttga ttttgaatta gtgatcagct atgaattagg
    18481 aggagacggc atcacggttc attatcaagt ggcaaatacc ggtgaaaaag aaatgttctt
    18541 ttctatcggt ggacatcctg ctttcaatgt acctcttgaa gaaggattgt catttgaaga
    18601 tttctatctg gcattttctc cattgaaatc aagattacgc ctaccattga aaggcccatt
    18661 catcgatatg gatcaaaaga cgttaggtca gacaaatacg aatctggctt tgaatcgtga
    18721 actttttgag caagatgcct tggtttttga aacaaggggt ctaaatgcgt acgccattcg
    18781 aagtgaaaaa tcttcccata gcgtaacttt aagctataaa gatatgcctt atgtagggat
    18841 ctggtctcct tatccaaaag aagcgccttt cgtttgtatc gaaccgtggt gcggaatcgc
    18901 tgacacggtg aacagtagtg gaaacttgat ggaaaaagta gggatcaatc aactaggtgc
    18961 ccatgaaatc ttcaagacaa aatattccat cacagtgaag taagaggttg tgaccgagaa
    19021 gttagcaggg aacaccgttt accaaggtgt gtaaaaaagc tatcctgtaa gtaataaggg
    19081 agtgtgacat cgatttgtca cactcccgtt ttatctcttt ggaatgaaaa agtatcagga
    19141 ttttttcttg ctgctattca aaccaaacgg gattcgactt tcagtatggt ttctgatacg
    19201 cttgatattg tctttatgcc gataaaagat aaaggaagaa actaaaaagg caatgatcgt
    19261 cagtaaccaa ttgggcttag ccaaaatcac aggagctaca taaggaagga cgatcgtaga
    19321 taaagtgatc aatactgcac tgatcatact gctcaaactc accatacttg tacaaaataa
    19381 actaacgata aagatgatac aagaataaac aaaaaataca ggattaaatc ctaaaagcat
    19441 tcctgcactt gtcgcaacag cttttcctcc tttgaaccca gcaaaaatcg gaaatgtatg
    19501 gccaagaatg gcgcataccc caaaccaaag agggttaatc ccagaaaggt ggaagatcat
    19561 cggcaaacaa gtagccaatg taccttttaa aatatccata agcaggacaa caatcccggc
    19621 tgttttgcct aatacacgaa atgtattggt cgttccagta ttaccgctcc cgtgttgtcg
    19681 gatatctttt ttgaaaaaca atttgccaat ccagacaccg gaaggaatag aacctaaaag
    19741 atatgctaaa ataagtaata agatcaattt cattttttac cctctttcaa gttgaaaact
    19801 atcctttatt ttagcatgaa taggtaacag gaacaattga aaaaatgaaa gatacaagta
    19861 taatagatgg aataaaaaga aaagcaaggg gtggcagaaa tgtctgtaaa aaatccaaca
    19921 caagaaacga ttttcaacta tttggaagaa gcaaaaaata ttgccgtcgt aggcttgagc
    19981 aataaaccag aacgaacaag ttacatggtt gcacaggtat tgcaagacaa tggatataaa
    20041 atcataccag taaatccatt attggctggt gaagaaattc taggcgagaa agtctatgcg
    20101 aatttgacag aaatcgatga tccaatcgat atcgtagatg tatttagacg gagtgaattt
    20161 ttacctgaaa tagcaggtga ttttttgaaa actgatgcta aagtgttctg ggcacaatta
    20221 gggattgaaa acaaagaagc agctgagatg ctaagaaaag caggacgtac agatatcgta
    20281 atggatcgtt gtattaagat tgaacttggt aagttgaaag aaaaccaaaa ataaaaatga
    20341 ccacaaagaa gagatgatcg atagagggga atgtcgaaaa agcggttagg agataattaa
    20401 aattgatttc tcgtctaccc tctttttttt cacgaccttt tctagtatga taatcgtggt
    20461 gcggttttgc cgcaaagtat tattgaagga gcgtttgcct tggcaaaaaa agtaaacaac
    20521 gaatacaatg actcgtccat ccaagtattg gaaggattag aggcagttag aaaaagacca
    20581 gggatgtaca ttggatccac tgacagccga ggcttgcatc atttagtcta tgaaatcgtc
    20641 gataatgcag ttgacgaagc attatcaggt tatggcagtg agattgatgt gaccatccat
    20701 gaagacaata gtataactgt tgcagatagc ggacgtggga tgcctgtagg aatgcatgct
    20761 tccggaatac caacagttga agtgattttt actgtgctgc atgctggcgg aaagtttggt
    20821 caaggagggt acaaaacttc tggtggactg cacggtgtag gagccagtgt ggtaaatgcc
    20881 ttatcgaaat ggctaattgt aacgatcgtt agagatggcg tagagtatca gcagaaattt
    20941 aaaaacggcg gtaaacctga cggtactttg aaaaagatag gaaaaacaaa aaaagcaaac
    21001 gggacgactg tccatttttt accagatgat acaatattca gtacaacgaa attttcatat
    21061 gaaattttag ccgaacggtt aagagaatct gcctttcttc taaaaggcgt taaaatctct
    21121 ctttctgatc ttcgtggaga agaaccagtc aaggaaattt ttcattacga agaaggaatc
    21181 aaagaatttg ttgattatct gaatgaagaa aaagatacat tgacaccagt tgtttatttt
    21241 tcaggagaaa aagaaggtat cgaagtagag gtcgcttatc aatacaatga cggttattca
    21301 gaaaatgttt tatcttttgt caataacgtt cggacaaaag atgggggaac ccatgaagct
    21361 ggtatgaaag cggcgatgac gaaatcttat aatgaatatg cgagaaaagt tggtttgctg
    21421 aaagaacggg acaagaatct ggaaggtagt gatttcagag aagggttagc tgctgtactt
    21481 tctatccgtg tacctgaaaa tcttctgcaa tttgaagggc aaacaaaaga aaaacttggt
    21541 acgccagttg ctcgtaccgt agtagataac gtcatcagtg aacaaatggg cttttatttg
    21601 caggaaaata gcgaaatgag ccagatgttg gtccgtaaag caatcaaagc cagagaagcg
    21661 agagaagcag cacgtaaagc cagagaagaa agccgaaatg ggaagaagcg taaaaaaggc
    21721 gaatctcttc tttcaggaaa attgacaccc gcacaatccc gaaaccctaa gaaaaatgaa
    21781 ttgtatttag tcgaagggga ttctgctggc ggatctgcta aacaaggaag agacagaaag
    21841 ttccaagcaa ttctaccttt acgaggaaaa gtcatcaata ccgaaaaagc taaaatgcaa
    21901 gatatcttga aaaatgaaga aatcaatacg atgatctata cgattggtgc aggagtcggt
    21961 cctgagtttt ctatcgaaga ctgtaattac gacaaagtaa tcatcatgac cgatgcggat
    22021 acagacggcg cccatattca agtattactg cttactttct tttatcgtta tatgaaaccg
    22081 ctgattgaag ctgggaaagt atatatcgcg ttaccgcctt tatataaagt atccaaagga
    22141 caaggaaaga agcaagtcat tgaatatgcg tggacagatg acgaattggc agcaatgatt
    22201 aaaaaagtcg gcaaaggata tatgctgcag cgatataaag gacttggaga gatgaatgcg
    22261 gaacaattgt gggaaacaac gatggatccg acttcccgta cgttgatccg cgtccgtatc
    22321 gatgatgctg cgcaagctga acgtcgagta accacactga tgggtgacaa agtagaacct
    22381 cgaagaaaat ggatcgaaaa tcacgtgcaa tttacgctcg aagaagatgg cagtatctta
    22441 gataaaaaag aggatactga aatctctcct tcagtttcta atgatttatt agatgaagaa
    22501 cgagcagata aaaatgaaaa caatcagtta tttgaagtag aataggaggt cacctgatgg
    22561 aaaatcgaca agaagtacaa gaattaactt tagaagaagt gatgggtgac cgcttcggac
    22621 gttattccaa atatattatc caagaacgtg cactgcctga tatccgtgat ggattgaagc
    22681 cagtgcaacg ccggatatta tttgccatga acaaagatgg caatacgtat gacaaaggat
    22741 tccgtaaatc agcaaaatct gtcggaaata tcatggggaa ttatcatcct catggtgaca
    22801 tcagtattta tgaagcgatg gtccgcatga gccaagactg gaagttgcgg gaagtactga
    22861 tcgaaatgca cgggaataac gggagtatgg acggtgatcc acctgctgcg atgcgttata
    22921 ctgaagccag attatccaag ttaagtggag agatgctagc agatatcgag aaagaaacag
    22981 tcgatctcgt gtggaatttt gatgatacag aaaaagaacc aacagtcctt ccagctcgat
    23041 acccaaacct tctcgtcaat ggatcgacag gtatatctgc tggatatgca acagaaatcc
    23101 cgactcataa cttagctgaa gtgatcgatg gcacaatcta tatgatcgat catccacaag
    23161 cgagccttga aaaattgatg gaatatattc ctggacctga ctttccaaca ggagggatct
    23221 tgcaaggaaa agatgaaatc aaaaaagctt atgaaacagg acgaggaaaa gttattcttc
    23281 gttcgaaaac aaagatagag gcaatgaagg gtaataaaca gcaaatcgtg atcacagaaa
    23341 tcccatatga agtaaataaa gcgaccttgg tgaaaaaaat ggatgagatc cgtttgaata
    23401 agaaaatcga tggaatagct gaagtacgag atgaaagtga tagaacaggt ctgcaaattg
    23461 tcgtcgaatt gaaaaaagac gccaatgctc aaggaatttt gaattatttg ttcaaaaaca
    23521 ctgagctaca gatcaattac aatttcaata tggtcgctat cgaccatatg actccgcatc
    23581 aagttggact gaaagacatt ttaagaagtt atatcgagca tcgaaagcaa gtcataacaa
    23641 aacgtagtca attcgattta gccaaagctc aaaaaagaca gcatatcgta gaaggattga
    23701 tgaaagcttt atcgattctg gatgaagtaa tcgcaacgat tcgtgaaagt aaagacaaaa
    23761 aagacgccaa aaagaattta gtcgacgtct ttcaatttac agaagaacaa gcagaagcaa
    23821 ttgtcacatt gcaattatac cgtttgacga acacggatat cacagagctt caaaaggaat
    23881 ccgaaagctt gattgcgcaa atcacagagt tgaataaaat cctttcaaat gacaaagagc
    23941 ttttctctgt tatgaaaaaa gaattgcgag aagtgaaaaa aaaatattct tccgctcgtc
    24001 taacgacaat cgaagacgaa atcgaagaaa tcaaaattga tacgcaagtc ttagtggcac
    24061 aagaagacgt aattgtcagt gtgacgcgag aaggctatgt gaagcgaaca agccttcgtt
    24121 cttatagtgc ttccaaacca gaagaaatcg ggatgcgaga aggcgactac ttgctatact
    24181 caggtgaatt gagtacattg gaccatgttc ttctgatcac aaataaagca aatgtgatct
    24241 accgccctgt ccatgaacta cccgatttga aatggaaaga tgctggtgag cacatttctc
    24301 aaacaatctc caatctgtct gtagacgaat cgattttagc tgttttccca tatcaaaaaa
    24361 ttcaagcaga gaaaacattt gtattcatca gcaagaatgg attgatcaag caaacacgaa
    24421 tgacagagtt cgaaccatgg cgcacctata agagccgtcc gctcagctgt atgaagctaa
    24481 aggcggaaga agatgaactg attgctgtgt atctgaaaga agatcagcca ggcttagatg
    24541 ttttcttagt aacgcatcaa ggaatgggtt tgcgctatcc tttaacagat gttcccgttg
    24601 ttggagcaaa ggcagctgga gtgaagtcta tgaatttgaa agatggagat aggattatca
    24661 acgggctgct tgtgttagca gaaggtgata cgccgatcgt aatcgttact cagcgaggag
    24721 ctgttaaacg catgcttgct caagaaatca gccaaacttc acgtgcgaag cggggagtga
    24781 cggtcatgcg tgagctgaaa aaaaatccgc accgtattat ctatatgtcg gaaggaagat
    24841 caaaagaaat aaccttgatg aatcaaaaag ggcagcagtt aacgattgac ccaactgatt
    24901 tccctatagg tgatcgaaca tcgaatggat catttgcgat ggatgagaaa aaaggtggag
    24961 aagtcatcga agttatcgat gctcctgaga ttttcatcga gtcgtaactg ttgtagtatt
    25021 ttaatgaaga gacgccatct gtattaatac aggtggcgtc tctttttcat tcatttttaa
    25081 taaatcctta tacgtttgca aaaaaagatg aaagtgttca caatgcttaa atgttggtat
    25141 gtcaacgttg atttaaatat atgtaaataa aacataaaaa ttgttaaata aaaaacaaga
    25201 aaacccttgc aaagataggg tgctgtgtta tactaatcac gtaaaaactg ttacataaaa
    25261 cagaaattta ggaggtccat acaatggagc aatggaatgg atttaaaggt accaaatgga
    25321 aagaaggcgt agacactaga gactttattc aaaataacta cactgaatat cgcggtgatg
    25381 acagcttttt ggaaggccca acagaagcaa cagaaaaatt atgggatgaa ttgcaaaagt
    25441 taaatgatat ccaattcgag cgtaacggtg tctatgatat ggacacagaa atcgtatcaa
    25501 ctattacttc acacgatgca ggctatttgg ataaagattt ggaaaaaatc gtcggtctac
    25561 agacagacaa acctttgaaa caagcattca tgccgttcgg cggaatcaat atggctaaca
    25621 atgcgctaaa aagcaatggc tatacaccaa acgatgaatt gacacatatt ttcactgact
    25681 ggcgcaaaac acataatcaa ggtgtatttg acgcttatac tgctgaaatg cgtgctgctc
    25741 gtaaaaacaa aatcattaca gggcttccag acgcttacgg tcgcggacgt atcatcggtg
    25801 actatcgtcg tgtggcattg tatggaatcg actatttgat ggaacaaaag aaaaaagatc
    25861 atgatatgac tggttatcat acaatgagtg aagaagtgat tcgtttacgt gaagaaatca
    25921 ctgaacaata tcgtgcatta gcgcaaatga aagaaatggc tgcgaaatat ggctatgata
    25981 tttctaaacc agctgcaaat gcaaaagaag ctgtccaatg gctatacttc ggatatttag
    26041 cagcaatcaa atcacaaaat ggtgctgcga tgtcaatcgg acgtatctca gcattccttg
    26101 atatctatat ccaacgtgat ttggataatg gagtaatcac agaaaaagaa gcacaagaat
    26161 tgatcgacca cttagtaatg aaattacgta tggtcaaatt cgcgcgtaca cctgaatata
    26221 accaattatt ctcaggattc cctatctggg ctactttatc cattgcaggt atgggattag
    26281 atggtcgttc attagttaca aagaatgatt tccgtctgct tcatacatta acaaatatgg
    26341 gaccttcacc agaaccaaac ttgactgttt tgtattctga acaccttcca gaaggattca
    26401 gaacatatgc tagcagaatc gctatcgaaa gttcttcgat tcaattcgaa aatgataatc
    26461 agcttcgtga acaatggggt tctgatgatt gtgcgattgc ttgctgtgta tcaggaacag
    26521 ttgtcggaaa agacatgcaa ttctttggcg ctcgtgctaa cttggctaaa acactattat
    26581 atgccatcaa tggcggagtc gatgaagtga ctggcgccca agttggacca gaatatcgtc
    26641 caatcacatc agaaatacta gactttgaag aagtgaaaca taacttcatg aatatgatgg
    26701 attggttagc agaactatat gtcaatacgt tgaacatcat tcattatatg catgataaat
    26761 attcttatga agcagctcag ctggctttga tggattctga cttgcaacgc acatttgcta
    26821 caggtattgc tgggatctct catgcggcag acagcttgtc agcgatcaaa tatgcaaaag
    26881 taaaagcaat ccgtaatgaa aaaggaatag cagttgattt tgaagtggat ggcgacttcc
    26941 ctaaatacgg aaatgatgat gaccgtgcag atgaattagt ttcatggatc ttggaatact
    27001 tcatgggaca aatcaaacgt cataaaactt accgcaactc aaaaccaaca acttcattat
    27061 tgacgattac ttcaaatgtt gtttacggta aaaatacagg aaatactcct gatggccgtg
    27121 aagctggaaa accacttgcg ccaggagcaa atccaagtta caatgcggaa caaagtgggt
    27181 tattagctag cctgaactca acagctaaaa tgaattacgg ctgtgcattg gatggaattt
    27241 caaataccca aacaatcaat ccttcaggtc ttggaaaaga acaagataca agaatcgaaa
    27301 acttgcgtaa tgtcttagat ggatacttct caaaaggagc ttatcatttg aatgtcaatg
    27361 tattcagtaa tgatttattg cgtgatgcaa tggaaaatcc aatgaaatat ccgaacttga
    27421 cgatccgggt atctggttat gcagttaaat tccgtgactt gacaagagaa caacaattag
    27481 atgtcttgat gagaacttca cacgacagaa tgtaaaaagt tcgtgaaaaa agtttttcga
    27541 cttagcgtcg aaaggacgtt gaatagccat cgcatcaaaa tcgaataatc agtatgctta
    27601 tttgaaagaa acgatttaca ggggcaggac ttgtatgttc cgtccctgta aattttacac
    27661 ataacgaagc ggcaattgcg aaaaattttg ttcagaaagc ttcgcttcga cagctaactt
    27721 gtacttaaaa tgaaatcaaa gaaaagatgt gaacttaaat ggaagaaaaa acaatcggct
    27781 atgttcattc tatcgaaact ttcggttcag tagacggacc tggactgcgc tttgtcgtgt
    27841 ttatgcaagg atgccgcatg cgttgccaat tttgtcacaa tccagatact tggaatattg
    27901 gcggagggaa agaatacaca gcagatgaac ttttagataa agcagaacgc ttcagacctt
    27961 actggggaga taaaggtggt attactgtta gtggtggaga acctctattg cagatcgatt
    28021 ttttgatcga actatttaaa aaagcaaaag agcggaagat gcatactaca ttggatacat
    28081 gcggaaaacc ttttacgtat gaagaacctt tcttttcacg ttttcaagaa ttgatgaagt
    28141 atacagattt actgttattt gacatcaaac acatcgataa tgaagagcat aaaaaactaa
    28201 cgcattggga caatgaaaat attttagaaa tggctcaata tttgtcaaaa atcaacaaac
    28261 cggtttggat tcgccatgta ctagttcctg agcgaagtga ttatgatgaa tatttgatcc
    28321 gactagacaa ctttattcaa accttgtcaa atgtagacag agtagagatt cttccttatc
    28381 atacgatggg aaaatacaag tgggaaacac ttggtttgaa atatccatta gaagggatcg
    28441 aaccacctac aaaagaacgt gtcgaaaatg ctaaacgatt gcttcataca gaagagtata
    28501 caaaatattt aacgcgatag gtcgcatcgg aaagaagata agtattgctt ctttccgttt
    28561 ttttgctatt attaaatagg agaaatcaca aaggagcgaa tgacatgtca aaaattttag
    28621 tttttggtca ccaaaatcca gatacagatg caatcggggc agccattgcg ttttcacact
    28681 tgcaaaatga attaggtaaa gaaacagaag cagttgctct aggtacacct aatgaagaaa
    28741 cacaatacgc attagaccac tttcagctat ctgcaccacg agtagtagag tcagctaaag
    28801 cagaaaatgc agatgtcatg cttgtggatc ataatgaatt ccaacaaagt atttcagata
    28861 tcagcgaagt aaatatttta gctgtagtag accaccatcg tattgcaaac tttgaaacag
    28921 ctaatccatt gtactatcgt gcagaaccag ttggatgtac aagtacgatt attttaaaac
    28981 tatataaaga aaaccaagta acaattccta aagaaatcgc aggaatcatg ctctcagcta
    29041 tcatttcaga tacattgttg ttcaaatcac caacatgtac gcctgaagat gaagcagctg
    29101 caaaagaact tgcagcaatc gctgaagtcg atctaaacac ttatggactt gaaatgttga
    29161 aagcaggaac aaatctaagt gataaatcag ctgcggttct tcttgatttg gatgcaaaaa
    29221 gtttcccaat gggcagttcc aacgttcgta tcgctcaagt caatacagtg gaccttaatg
    29281 aagtgatgga tcgacaagca gagctagaag cagcaatgga agatgaaatg aaagcaaatg
    29341 gctacgatct gttcgtatta gttgtcacaa acatcttaga cagtgattca gaattacttg
    29401 ttgctggtaa aggaaaagaa aaagtagaag aagctttcca aacaactttg accaacaacc
    29461 gtgctttgct taaaggtgtc gtttcacgta aaaaacaagt cgtacctcaa ttgacggctg
    29521 tatttagcta atcaaatggt agtatgaagc attcaaaatg aatggataag cgattgatat
    29581 aaaaagaaac atagaacata ctaaaggccg aagaaatgga ggagaataac agtcaattat
    29641 gactcttgtt cttcctcctt tttacgtaaa agccttttaa gtaaaataaa acggatagaa
    29701 aagagaacat atatgaacta ttattcaaaa aataaggaaa acttttatca aaaactgacc
    29761 gatgatccgc ttttttcttc attgaccgat tatctatatg aacatcgaga acaagaaacg
    29821 attctaagag aattgaaaaa agaattttcg caaaataaat tttcccattt tcttgattta
    29881 ttgatcgatg caggattaat caagagggaa gaacgaagat atcatttgaa ctttccaata
    29941 tttgatccta aagattactt gcaacaagcc acttcggctg ctgaaaccat agctgaacaa
    30001 ttgaagagac tctctgttga tgaacaaaaa ttagcgatgg gagaagttat ttgggcgtat
    30061 tgtttcgaag atgaacggaa agaagcgtat ttttatggag taagaaacag ccgagagaca
    30121 gaattgctga ggacaactgc aggaaatgag aagtaccgtt ttatcacact ttcctctata
    30181 gaacacttcc cgcttacttt ggctaattac ttttttgtac agaagaatca gcttcctgta
    30241 acgaaggcat tcaaagaatt agccgaactt attggagacg taaatgaatc gtactttttt
    30301 gatcaaatag aagttatcgt tgacaggata cgtaagaata aatataaaaa tcgccggcct
    30361 agtattttcc atcagtctct acttgtgacg aatactataa aggaagaaga atcattcaca
    30421 ttagaattac ctattgtcga aaaaaacaat tttgagattg aattaccaac attggatcct
    30481 tcattgacaa tggaggaaat ggcctttctt aaacgacaaa tcttttctga gctgagcaag
    30541 aaatttatac cgcatgcatt ttcttatatc aaagaatatg gaacagtttt agtgagtaaa
    30601 acctaagaga gcagtaagca aaaattatac tgactcttgc aggaggagcc tttctcttcc
    30661 tttatggtta aaagaattat ttgagtattt tgcctatcta ccacctatta gattcactac
    30721 atggtctgga aacaaaggaa tgcaaaaaaa tggtgagaaa tgtggaatga caaaggaagt
    30781 agtgataaga aaagttagat ttttaaataa tcaatattat gattcagtca agtatggcat
    30841 tttatgggaa gaactagctg attagattag gaagtccatt tgataatcca gaatcactaa
    30901 gaaacgaatg gcgagtcatt aataatttat ccaatcttgg gttattcttt tagattgaag
    30961 tgtttaaaac taaaaaagca tttatgaagt cgtaatagtc aggcttcaaa atgcttttta
    31021 ttttgtttgt tttttctttg ccacataaat acacctagac tgacgatgaa gagcatatcc
    31081 aacagatttc ctgcgacttg gatcgtcgaa gggttggtca gtggcattaa agcaaggaca
    31141 aaaaaggaaa acaagctgta aataaaacag gtgacaggaa ctaatcgaaa gaagagcgga
    31201 catttcaatt ggctaaaaat gagtgaaaaa ataaaataag taaaaatgga taaaatcacg
    31261 actaaaccat tcatgataaa gaagaaaagc gtattgtgat taaacgaata aaaaagaatt
    31321 gctaaaaaag aaaacgtttt aaatgcaatc aatacgcttg aagcttcgtt taacgtacca
    31381 ggatgtcgat gaaaaaaata ggcaccgaaa taaacagaaa acagtaagcc gcaaagagaa
    31441 gacaaaatca aaaaaaacgg attttcttcg gcttctgtga tatcagaatg atccaaaagc
    31501 aaccagccta atgcactaga aggcgcatgt gaggtcttaa ggtatgaata acatagaaaa
    31561 aataagatcg tcaaagttgc aaaaaaagag cctaaatgta aaaaattagt ggttctacgt
    31621 tgaatttcca taatatctcc aaattaaatt gataagtata taaagtataa taacaaattg
    31681 atgagtatat gtcgatgttt tttaatcaaa ataataaata aaaactgtct aacatttaaa
    31741 atttaattta gatattagac agtttttaag tttgtgagtt attttccttg tcgttttctc
    31801 acttttttag tccagaaacc acctgaaata tattttggct cataagaaat gataaaagca
    31861 cgctcttcga tttctttgat caggcgatac agttcacgtt cgctttttct aggagaaagt
    31921 atttcgagaa ctagccgctc accttccagc cccattgcag aagtctgtgt cacaccataa
    31981 ccgtgttctc tcagtatacc cggcaaactt ttatcttctg cgctatttgc tggcaaaatc
    32041 gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc gacactgata
    32101 cctactgcgt aacctaacgc atagacaaat aaattcagtg gattatctaa acgatctaaa
    32161 acaagggata agccgacgat ataaatcgtg atctcgacca tactgacaag tggagcaatc
    32221 agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa
    32281 ttaatgagga agatcatccc caacattttt ggatcaatca tttgtaaaac tcctttcatt
    32341 tatataacaa caatatcaaa aaaaatcgta agtgaagaaa aattgaaact ttatacatac
    32401 aaaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga ttaaaatgaa
    32461 aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca gtatatactt
    32521 aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc agatgtcgct
    32581 gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg gtaatatttt
    32641 cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc tagcattagg
    32701 agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg catttatatt
    32761 atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat ggcgatcagg
    32821 aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac gttctgtcca
    32881 tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta ttcccgttgt
    32941 catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat
    33001 tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca
    33061 gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa atctgtttga
    33121 atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc agcttgcagt
    33181 tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac atcctcatat
    33241 agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa aagaaatcgt
    33301 aaggcgcgca tcggaatata attatgtcgt accaaaagac tattttgatt tgatttcatg
    33361 ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc ctctgttctt
    33421 ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa
    33481 aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca
    33541 tatggtacaa ataccactaa tggaagaagc ttccactttt ttagcaaaaa atttgataaa
    33601 aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt ccgtttttta
    33661 ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa
    33721 catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt
    33781 tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa
    33841 gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca
    33901 cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact
    33961 gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat
    34021 tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc
    34081 acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg
    34141 tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca
    34201 aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa
    34261 gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt
    34321 gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg
    34381 acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta
    34441 gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt
    34501 gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc
    34561 aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa
    34621 gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat cagagagaca
    34681 acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt ttcttaaaat
    34741 aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa agttccattt
    34801 ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt tcctgcgtac
    34861 gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt tgacggacaa
    34921 gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga atttcatttc
    34981 acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg attgaagtca
    35041 gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat taccggatta taccggccat
    35101 taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc ctctcgttct
    35161 gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc atctgttgac
    35221 acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc gattatccca
    35281 gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa aaaaacggat
    35341 gactcttatc tgttgttcga tacagaaccg acgccgtatc aagaaaaaag aaatggtgag
    35401 ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg aaacaaagca gtcagaagaa
    35461 aaaaaatcga aaaaaagcca tggtatctta gaacgctcat tacaaggatt gatcgatgat
    35521 caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa atttatattc
    35581 ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta tccgaaagtt
    35641 tatatcgtat cctcaaattt ttttgagttt aagcgaattt tttctcccaa taagtaagaa
    35701 aataatggca aaata
//
LOCUS       DACCSK010000014        35376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCSK010000014 DACCSK010000000
VERSION     DACCSK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.014"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            161..724
                     /locus_tag="IVP39_000625"
     CDS             161..724
                     /locus_tag="IVP39_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585722.1"
                     /translation="MIKKTQNKIQRICQKHFVSRLNSNNETTSTTSSTQLIDPTEKEI
                     TAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVAVNGSMSAD
                     KTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNTIDSVTPNA
                     DKSYSFTPSTWGGLPAGGGMSTFSIGI"
     gene            926..2050
                     /locus_tag="IVP39_000626"
     CDS             926..2050
                     /locus_tag="IVP39_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585723.1"
                     /translation="MGDKVVITPTAVTGYSFTQTNGSLTALASNVNTVTYTGKQAPIT
                     IKYQDYFGQTIAPPKMMNITYGSAAQDLTTNAPTINGYTFTAVSATETKNQSAMSVSA
                     SLDTNGNVIVKDANGKQISEVILYYKTNATVSINANGSKYYDGLSVVPIVKYTFNDKT
                     ESTSLLNNDLSIDQITWNPTDFKAMNEKGVEVTEPTEIGTYTSWQLTKSGLAKLAART
                     NYLFTIVQTSDVYTIKQISGAVELGDSKTYDGKAGVPSIHVKLAEGVTSSLTPVQVAL
                     SSMDYTVDAQSAKNMVNAGSYTIKLTNSGIDKVKAANPKLSFTDIANTSGTYTIYKAN
                     AVITVDDAAFNYDAQSHSIPVGNVHVTGVLRRIIGLYPNS"
     gene            2028..2288
                     /locus_tag="IVP39_000627"
     CDS             2028..2288
                     /locus_tag="IVP39_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7585724.1"
                     /translation="MDYTLTVNSRTDIGSQIVTISLGEETVNNNYTITATDTAKLTIN
                     PTLLLPSTGGIGLLPFVFLGMIFIFSGFFYFIHRKKKAGEQR"
     gene            2285..3787
                     /locus_tag="IVP39_000628"
     CDS             2285..3787
                     /locus_tag="IVP39_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7585725.1"
                     /translation="MKKTKNVRVDIFAVLFFILLVFFIGSPTVSARESPITFTLHNRM
                     FESDKMPSISQNDGINIPDNFQEAKGLNGVTFAIYDVSDEFYKLRSEGSSVEDAQRKL
                     AQKSDSEKILAENVTKTVDGEEGIASFSASDKDEQGRDAVYRFAEIKTSDQVKEKSAP
                     FVVVLPVTNSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDKVPYLITVT
                     IPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFVLSDSGKDL
                     SSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQFVKVDRQT
                     KQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHGLSFGSYEL
                     VEIKPPKGYILSQSTILFEVEKSSYDPLHPIPLEIINEPKANPTNNGKTPVQMQTNGP
                     SNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK"
     gene            3784..5175
                     /locus_tag="IVP39_000629"
     CDS             3784..5175
                     /locus_tag="IVP39_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7585726.1"
                     /translation="MKNIKPKIWTFLGTAEVSAAVSPTPENVTVNLHKLKFTSAPENQ
                     INNGTELTFPNSEPLNGVEFNEYDITATYYPSKDTAVPADATPFASVTTSGEGLANLT
                     LPGKSDGKDAVYVFVETPKPGVETSPNIVLSLPFYNPNSSDPDKPLDTIHLYAKNVIK
                     ESNIKIQKAGNFSGVDKLAGAQFQIKNSENQFVTGFDENTGVALYDPAESALTFTTDT
                     EGSISVNGLIDGNYTLVETKAPDGYQLTDPQKETTFIVKNGKLQQTTGIIHGADEANL
                     GNTNGVVNRENTTTDNTITVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVK
                     LMKKIANLASGEYPYAWSDEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPE
                     GYALPKNEAAYTEFTVDDDPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGA
                     VIYFKKRHQETEA"
     gene            5383..6555
                     /locus_tag="IVP39_000630"
     CDS             5383..6555
                     /locus_tag="IVP39_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7585727.1"
                     /translation="MNKSRIRILDIFMAIILVAGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGGNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6717..7211)
                     /locus_tag="IVP39_000631"
     CDS             complement(6717..7211)
                     /locus_tag="IVP39_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585728.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            7800..8222
                     /locus_tag="IVP39_000632"
     CDS             7800..8222
                     /locus_tag="IVP39_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7585729.1"
                     /translation="MQTFLTKVSQNKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNDNNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8388..9581)
                     /locus_tag="IVP39_000633"
     CDS             complement(8388..9581)
                     /locus_tag="IVP39_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7585730.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLILTTLTGVTALR
                     ESLQH"
     gene            9805..10182
                     /locus_tag="IVP39_000634"
     CDS             9805..10182
                     /locus_tag="IVP39_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7585731.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10170..10508
                     /locus_tag="IVP39_000635"
     CDS             10170..10508
                     /locus_tag="IVP39_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7585732.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10498..10611
                     /locus_tag="IVP39_000636"
     CDS             10498..10611
                     /locus_tag="IVP39_000636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7585733.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            10642..11085
                     /locus_tag="IVP39_000637"
     CDS             10642..11085
                     /locus_tag="IVP39_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7585734.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSSVVNELIHRFKEAFEQ"
     gene            11334..11654
                     /locus_tag="IVP39_000638"
     CDS             11334..11654
                     /locus_tag="IVP39_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7585735.1"
                     /translation="MKRFRKCLTLVFVLSCLIQLSAFKQVVFAASEKSSVGITFEGSV
                     PQSSTDPSVPSSQDAEQSANQSRQKKYPKTNEIQQSFITWGGIFLLVISLLLIFWKRK
                     RNRE"
     gene            11651..12310
                     /locus_tag="IVP39_000639"
     CDS             11651..12310
                     /locus_tag="IVP39_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7585736.1"
                     /translation="MKKTILAAALISTTLFAGTLAVHADGLNGKSTAKIGLTAQDPDH
                     PVGPIDPIDPDDNPPSNDPTGNTGDLRIDYMSKIDFGKQTISGKTETYTAEKPALTTE
                     VQVSDLRGTGAGWNLQVSYDTEKPGFTSEDKTLNGAELTLPVGTPETVEDNSSAAPAT
                     SKVVVNNDAQNIMSAAANTGLGTWADEMDTANVSLKVPSGNLVGDYTATLVWTLSDAP
                     A"
     gene            12352..13062
                     /locus_tag="IVP39_000640"
     CDS             12352..13062
                     /locus_tag="IVP39_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7585737.1"
                     /translation="MKKQVFYTLAATVLLSSSLLSTLNVQADETEPTKDSATSTAKIT
                     FTGDNSDPVNPVDPDDPDNPVNPVDPDDPDNHGTGDNGPLSIDYVSNITFGTKDISTT
                     SQVYNAENAEPHVQITDKRGVEGGWTLTAAATKFVASDNSELKGAELLFKNGVAETTS
                     DNTSKAPVTSDLIFDNTEAKTVMTADSLKGQGTWLDVFKGTEGNNTNVQLSVPSGSAQ
                     AKEYTSTITWTLTAGPTA"
     gene            13076..14092
                     /locus_tag="IVP39_000641"
     CDS             13076..14092
                     /locus_tag="IVP39_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7585738.1"
                     /translation="MNKLQSLFVAALSLLSIVVMPLTTYADSNESAYSVNANIPDFQK
                     NKNVSYFDLQLDPNQKKEISIHLANSAKEKAIFNVDVNNAATNSNGVIDYGQTDLKKD
                     SSAKYVLNQLVTPNNQEIELQAGESKDVTFQVQMPKDRFKGIILGGIHISKKGNVSEK
                     VDGTAIRNKYAYVIGVKLQNDTDAVTPDLKLNSAKAGLQNSYTTVFANVQNPTPGIIS
                     KVNFDAKITKKGSKDVLYETKKENMSIAPNTNFDFPISLNKEKIEAGDYTVTIDANEG
                     NTDKTWHLTTDFTIQSEKAKKINKEAVTEDKGISYLPAIITVGILLLLIIAFLSYKLL
                     KQKK"
     gene            14106..15842
                     /locus_tag="IVP39_000642"
     CDS             14106..15842
                     /locus_tag="IVP39_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585739.1"
                     /translation="MKLNQPLNKLYVLGLLFIGAFSVIFQSTEVSAEANYPEIPEATI
                     FDGYVKASISYDKDHSPNYTAVGDTVTVTTTLEKLDDGVLPDKYKQSSVVILFSDETQ
                     GLQLKGEPEFKYELGGLEDTGKFVDPTKDLQTNVISVFNFSTKILYNDTATYDGRMKQ
                     VPISSTELDDGPYDDTYSLYKLLVAKGSKVTLSYKATVTEEALKNKQFSLHTAIYDPT
                     IGGGDFDWDHFLTTSMPSIQNLGISFDEATKDKKIDIKDDSSYQTTLTGSWAGAKDDI
                     HPVLTIDGKEVPIPDGSFKEDGTFEIPVDLKNIGNIGTNNVHIKISDSNGQVAEDDAA
                     ITLVQTLVPPQIKLSDSLANASISVFPIDQSLTIDGEWKDKDSNAVSLFYKLNGQERV
                     LQDNVSNTAKDSWLPFSQEISLADLNTGDNQVEVYAKDAEGQLSNHESFKIILNQGNV
                     SFKSINPEIQFNNMVISGTTVYSSPQATVGVVVEDTTGIPKDWQLKVKQTTSFSDGTR
                     DLPAQLTYLNGEEKLSINDSSAVDLPVVQNNATDYSLQQDANHQFRLAVSPGAYTGEY
                     QSQLEWTIERAP"
     gene            15919..17178
                     /locus_tag="IVP39_000643"
     CDS             15919..17178
                     /locus_tag="IVP39_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7585740.1"
                     /translation="MESIMFVLIAYLNLFIWMSLLFKGASKWRFLLFYLGLMIANMFF
                     VPYTFVFFAIAYAAMCWFLYLLVENIFLSILLQNFLLNVVGISFFLAIDIPRYLGFYT
                     SYLNLSVELVLATSLFLLIRKADQKYDIFDYFSGYTKKLKGLTILSVVIYVLIYIFHL
                     GISFYSLDYILTSIITLLYSIFYTAFFIVFIIYKKKFQVIELYLKDNQETKEYYEKLD
                     DFRHDYLNYISALEYSLMNDSQENSIKLLTHLKDYSLEAIDDARHNPLKRISDPAVRG
                     LFYHFMERANQSGISYDIQVLNIINNIKADYIDVLRLLSIALNNAIEHCDETRKEEPI
                     SITINQTKERFYFKIENPADMSSTSLAKLMKRGSSTKKNGGLGLYNFSKITDKYLNAN
                     YGIKYLKRTKMFVLELTIYEKSICKTV"
     gene            complement(17231..17980)
                     /locus_tag="IVP39_000644"
     CDS             complement(17231..17980)
                     /locus_tag="IVP39_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7585741.1"
                     /translation="MKIYYIEDNLFHQELLKKELLKHKELSALSLQIYPNSELETIYK
                     RLDTLVFSSSDLFIIDIDLKTLHSGIDFAKKIRNKNPECSIIFLSNDNTRGLEIINQQ
                     IRPDRYLDKSNLEEVAQSLKQLLLEKLEAQKQSSSIIELQGATKVFLIAPDEVNYIST
                     INGSRSTLEFYGTNENFVIHDKMKNIKQKFQDYHFFNDLKSFSINPFNIKEVDKQMME
                     VHFKNKDTLLLSSSSVKKLLRHIDQLKQTNG"
     gene            complement(18100..19245)
                     /locus_tag="IVP39_000645"
     CDS             complement(18100..19245)
                     /locus_tag="IVP39_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7585742.1"
                     /translation="MDLLYKKYLIPMVLFDSSGEVCHPKISWEIQPIMEKFISPKENQ
                     SVCLFNHFPFMFAKLDFITDSKPYSLLIGPCGMIGESPDTFLFKGHSYHYGIHYTKET
                     TAAFEEFVQLTYAFLTHELLKKEELHWVYQKEIKQRSTTDQRLEDSLYDRRMQEATFD
                     SYQFELRYTEYIKRNQPEKIDWLFKKMQETYHVELSENELEGLKLKFSAFVAILTRIS
                     IDEGVPINQAFSLSDALIQGLFRIHSSSEWHTYMKEATYRFMQLIHQQPLADKSMLVK
                     QLINYIDSHIYDRVTLDELAETFDKHKTHLSALFKKEMGQTIHSYIQERKINESKHLL
                     LFTDKSYKEISVLLSFASQSHFIQVFKKTTSLTPREYRDQHYAYSVH"
     gene            19453..21657
                     /locus_tag="IVP39_000646"
     CDS             19453..21657
                     /locus_tag="IVP39_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HAP7585743.1"
                     /translation="MDEKQLKQLVDSLTIKEKIAQTVQLNGDLFTDSDVMNTGPTKEL
                     GFPDDFDLSQIGSIYNINEPEKLKKIQKKIMEERNKKIPLLFMSDVIYGFRTIFPIPL
                     AQAGSYDFDLIQKAARETAKESYLSGLHVLFSPMLDLVRDPRWGRVMESPGEDVYTAK
                     EFAKHAVQGYQGDNLQLIDKYRVGACIKHFAGYGAPEAGREYNTVDMSNQRLFNEYLA
                     PYQAAVEANCLLVMTAFNVLNGVPSTGNRWLNRDILRKRFGFNGVLVSDYAAIEELQV
                     HGYAKDQADSAKKALEAGVDFDMMTSVYANSLEKLAEENKEILQLLDEAVWRILDLKN
                     KLGLFEDPYRGLEEENTGEILTEESKEIAVKLVEKSCVLLKNEDTLPLKKHQKIAVIG
                     PYGKSRLTIGFWASVSGKPQDSIPLKDGIEQHFSKEQLLFAKGFNLFDSYEPFGPLKA
                     GIEQLNGTIEDEETLFQEALETAEKSDVILLTIGEQFLESGEGASKTNLRLPEKQKRL
                     IKALAALGKPIVGILYTGRPLVLTEIEPYFSSLLLVWYPGTMGGIGIANLLAGKAAPS
                     ARLSMTFPRSEGQIPIYYAHASTGRPLETSTHSTRFVSKYIDESNEPLFAFGTGKTYG
                     KVEASWQEIQDQEDKLQLVYEVINHSAKPIETVIHIYVKDKTASIVQPVRRLIASQVV
                     SIHADEKKSINYTLSKEALMFYNNQGEKIWESGEFTFSLSTVDSEDDRTIQL"
     gene            21673..23019
                     /locus_tag="IVP39_000647"
     CDS             21673..23019
                     /locus_tag="IVP39_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7585744.1"
                     /translation="MEKLEQFLSSRLLPMSEKLQKNKVLAALMEGFIRTSPITLGIAF
                     ITIIGNFPFPGWIDFLKNVGIFPHVEAITNGATGVFSLYVVYSLAFSYAKQLGTNERN
                     ASIISLASFIMLMPQSVMTTVTQDGKAVEQAVGALRLDYLGGQGLFIGMLVALLITRF
                     YAFLAKKNIMIKLPESVPPMVTQSLSPVFVVTIIFVVVFALRVLFGLTQSGTIFQFFI
                     DVINAPLNSLVASPLSIIIIMELLAVLWFFGIHNAVLQGPLGAISMTMVVGNIAAFQE
                     GKALPYLVPSVIYMGMYAAGFMGFVTFFMIKSKSAKMRQLGKLSFVPSLFNVTEPLMF
                     GMPIILNPIFFIPQIFTQLIAGFVTWGLTTTILPISLNPTMSLLPWTTPTFVKMPLSG
                     GLNYTILMVICMAIGVVMWYPFIKIADKKELELEAALEKEEAETKAEKIINAEVEA"
     gene            23019..23933
                     /locus_tag="IVP39_000648"
     CDS             23019..23933
                     /locus_tag="IVP39_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7585745.1"
                     /translation="MKGKSMWTLSIDIGGTYIKSALIMDTQIREKRQIETPKTDKDNF
                     ILVLVELIYSYQQIEPIEFVGFSVPGAVKEASTVFFGGAVACLNEVNLKQEIEKHLPV
                     RVFVENDAKAAVLGEASFGHLKGIENGAGIILGTGVGVGLLLDGQVRKGPHCQAGEVS
                     FLIQDRGINGAESFAGINLSAVRLVKELAKLFQCEPEGPLVFDYLYQKEDEQAQTLYR
                     TYCNQVAILCFNIQCLLDLDKIIIGGGISKQKRLIRDIQKNYEAIFSVSPMIEQTITK
                     MTIEAAAFESEANLIGAAKGVRLNEISN"
     gene            23917..25284
                     /locus_tag="IVP39_000649"
     CDS             23917..25284
                     /locus_tag="IVP39_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7585746.1"
                     /translation="MRFPTDFLWGASTSAYQTEGAWDADGKVPSVQDMKEIPDGTSDF
                     KVAVDHYHRFEEDVRLFKELGLKAYRFSISWSRVMSNGKVNPKGLEFYKKLIALLKEN
                     EIEPIVTVFHFDLPYEIHEKGGWENRATIQAFADYCQLLFDTFGQEVNYWQTINEQNV
                     MALAGSIIGTSNKSMKEKFQENHHMLVAQALVTKNYHDGNYPGKIGPAPNIASVYAAS
                     DKPEDQLAAMNMSALRNWLFLDAAVYGVYNHNAWHILSKINACPEVTEEDKKIMLEGT
                     CDYIAFNYYNTMTVSAYYQKENKIDQQSGFGIPDFFQATTNPHLPMTEFGWPIDPEGF
                     RYTLNEIYSRYRLPLLITENGIGAKDQLTEDGKIHDKYRIDYLKQHIQQMYLAMEDGV
                     EVIGYCPWSAIDLISTHEGIEKRYGFIYVDRTDDSLNTLDRYKKDSFYWYQKLIAENG
                     LNEEE"
     gene            25289..26254
                     /locus_tag="IVP39_000650"
     CDS             25289..26254
                     /locus_tag="IVP39_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7585747.1"
                     /translation="MQINTMTIDNEANTTLTVYLHEEKPEFHRIDKRPFILVIPGGGY
                     GFCSEREAEPIALKFAAEGYHAGVLRYHVGEHRDFHKALADAEKSMDLIALLAEKAKI
                     DQSKVAVIGFSAGGHLAAALSTLSDRKPSACILAYPAILSSFAEAMKIKAPSLDMCVT
                     PQTPPTFLFSTFEDNVVPIENSILYLRALEENDVPFETHIFQKGKHGLSLGNKWSGNS
                     EEMVDPRFAKWFELCIEWLEIIWSQAAVETTQKIEDVWQVPIKNLYAIEANRSILLEH
                     LPFLADKSRYKIIRSLSLNQLCEFSPEKFTKTKISQWVNELTWNL"
     gene            26292..27848
                     /locus_tag="IVP39_000651"
     CDS             26292..27848
                     /locus_tag="IVP39_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RuvA"
                     /protein_id="HAP7585748.1"
                     /translation="MKYEIINKNNRFYFLMDEKPCLLLGGEVHNSAASTALSMEKNIW
                     PFVNNIGLNTLLVPVYWENIESIPGIFNFDLIEAQLQKAAENKIKLVFLWFGLWKNGL
                     STYVPPWIKTDTANYFRVVNQKQEQLNCISPFCEKAIEADCRALSSLCQFISQHPLKD
                     QVCMMQIQNEVGCLETARDYSRKAQQIYGSTLPERIRELYPQANSWQELSDETFMAYA
                     FSHALEKMALEAKKWLDIPLFTNAWLHKEGKSAGDYPSGGPTKNVLNVWEKNAPSLTA
                     FAPDIYEENFREIIQDYQMIDQPLFIPETRKDLRYLSNCFYAFGKGALLYSPFGIEDL
                     RKSTEAIIDEDQAFLKELGLDQAAFDPTGSLPYFTKCYQIIKELTPFLTDETIKIHSF
                     KKEKDMTYQYQDENYRFLIKYLSKDKKEQLNTAGLFFQLENDYFFAGCNFALFHDAVD
                     EELCSEILNFEEGYFEGKKWITERVLNGDERYTPYIGNMPKIIKFSLYTYPKKEQEVV
                     HEYLADERNL"
     gene            27817..29529
                     /locus_tag="IVP39_000652"
     CDS             27817..29529
                     /locus_tag="IVP39_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HAP7585749.1"
                     /translation="MNILLTNETFKPSTSMFAQEPEPLENLSVYRNSTAAFQLLLHDG
                     KKNHYSLDDQFTIPDAIEIPIYRIEINSELPIKSNFIEYYCGKDDIFYGDKLLEEKAH
                     TYSGDRFTPIYVEIPVDGQQKTGTYPIDITIYQAGITTKETVIGQKRLEVTVEAFTFS
                     DKVTDSFHLDIWQQPSNLSRTFQVPLWSNQHFELIQTMASTLAELGQKTITVIAGEIP
                     WKGWFNYIVKDFPANLYEYSMVKVLKTKEGNIQCDFSILDKYLACFFEAGIDKEIDVF
                     GLLGVWQPPFFPLNKAVEHPEKLVIRYYDEEIEQMAFIEKQEELRQYFQQLFTYFKKK
                     NIWDKVRLMADEPKAHEIATFKEAVKCLKEIEPTLQLKVAFDKEPVLQALISEIDYPV
                     TSFYCTCQNYEWLKEQFPGKTQYYICNYPDKPNTFLHSPLTESRLQGALAYYFGTDGL
                     LRWAYNCWPEHAREDIRYNTSSLPIGDLCLVYPSVSGSLLLSLRYKELYRGVEDFYLL
                     KEAEKINKTKTDQLLADFLGGKKPENWMENSHQSVQRLFNQSYSQFDQLRKKLIAIIT
                     ETSI"
     gene            29880..30887
                     /locus_tag="IVP39_000653"
     CDS             29880..30887
                     /locus_tag="IVP39_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7585750.1"
                     /translation="MVSIVLIATLMVLAGCRSSNQTVSGNQNQSSDTIKIAFYPNESA
                     AEFEPAREAVKAVVEKATDKKVELVTSTDYNVVVESIANGQVDLAYMGAEGYIEANKK
                     NEQVEAILTNSGPSGTLEDALYYSFLAVKPENSKEYEETGGYTINPIKDKTISFVTNT
                     STSGFTVPSNKIAEHFDLKNTDDLIMDGLFFEKVLFGGSHQGSMINLLKGDADVAAFM
                     NMPQYFDVIQGEENKAGVIYEVKKDAQTPFDTVRGEQAQVILSIPVLNGPIAANKQTL
                     SEADIDKIIKGMTADSTAENKAIFAPKDADVPGMFTKEGNERYVEVSDDFYDEIKEMN
                     K"
     gene            30912..31664
                     /gene="phnC"
                     /locus_tag="IVP39_000654"
     CDS             30912..31664
                     /gene="phnC"
                     /locus_tag="IVP39_000654"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP7585751.1"
                     /translation="MLLEVKNITKTYPNGNQALQDISFSLEKGEFVSIIGPSGAGKST
                     ILRTINRLIEADSGTIRFEDREMNELKRKELRKKRRRIGMIFQHYNLVSRLTVIENVL
                     HGCLGYKTTLSGAAGLYREEEKEQAFDLIEKVDLSAFAYTRCDELSGGQKQRVGIARA
                     LMQNPALILCDEPIASLDPKSAKTIMDYLKRITNELAITCLVNLHQVETAIDYSDRII
                     GVNSGKIVFEGRPYELTKEKIETIYQSKKLLV"
     gene            31676..32473
                     /locus_tag="IVP39_000655"
     CDS             31676..32473
                     /locus_tag="IVP39_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphonate ABC transporter permease"
                     /protein_id="HAP7585752.1"
                     /translation="MEKIEAEKFFRQQKRYRLFYILLIAFLYFISAHGVGFRAGAAFS
                     AVPKSFFWLFSNFIPTSDSLAYLSIIISRLVQTILMAVTATTSGAIFALIAAVIGSRE
                     IGLNRFTKVIARGMASFFRNVPIVVWAIILLLSFKQNEFTGYLAITFTTFGYLTRSFM
                     ETIDEVSGSVIEAMMASGASYLQIIWQGVLPMCLNQLLSWLVFLIENNIRDATLVGIL
                     TGTGIGFLFDFYYKKFQYDVVGMITLTVIIAVISLEAISNTVRRMIR"
     gene            32473..33360
                     /locus_tag="IVP39_000656"
     CDS             32473..33360
                     /locus_tag="IVP39_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7585753.1"
                     /translation="MMDYQTNETNAIFNPTSYSKVAFRKRNQRIKVKLWRQERIIVRL
                     MLLLLCLVSAYTVITIDYGNVKLTEAVSAFFSNMFNIFLRPQLSGRFTTFSLVESLGI
                     TIGLSVLTTVIGAFLAFFLSLLASRNLSSGFLSNTIRGLMSFIRAVPTILWVMIFSIL
                     IGLGANAAIIGMSFHSIAYLTKVYSESIEEMESGMIESLKACGASWWQIVFQSVLPHC
                     FTTLLSWTFIRFEINFTNAIAVGAAAGAGGLGFQLTMASNFYFDFREIGVMVYMILTI
                     AVLLEFISLKLKEKYIVKT"
     gene            33387..34202
                     /locus_tag="IVP39_000657"
     CDS             33387..34202
                     /locus_tag="IVP39_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HAP7585754.1"
                     /translation="MKADLHVHSCYSNGFFHPKELLSLATAQEINHLAIVDHHTFAGI
                     EAVKEVFFESPVNLIEGIEISAFDLKRQKRVRILGYNLSNRSYIKQLCRPLLHFQTNQ
                     TLFKLKKLQAEGYHLKEEELWKEAEKATALYPCHLYRLLKKRGYGTQMDHTFYQKLMT
                     AEEVSDNRKKIDVYDAIEAIKYGGGLTVLACPGKFDNYDLIPELASFGIDGLEKYHPA
                     HRIRDLKILTDFIKTYRFSCFGGSDFHGIRGTTGFGRTLITDAREFPDQLLYN"
     gene            34297..34791
                     /locus_tag="IVP39_000658"
     CDS             34297..34791
                     /locus_tag="IVP39_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7585755.1"
                     /translation="MINFSLSQIQDLPRIVEIYNQAIPTRLSTADLEPVTIESKKAWF
                     DAHQSKERPMWVMKIDQNIAGWVSLSDFYGRPAYAKTAEISIYIDSNYRKHGLGQKAL
                     SFAESQLLDCKIDTLLAFVFATNQASINLFRKNGFEQWGHLPKVANMDDQLTDLTILG
                     KSYE"
     gene            35158..>35376
                     /locus_tag="IVP39_000659"
     CDS             35158..>35376
                     /locus_tag="IVP39_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7585756.1"
                     /translation="MSKQSFLVIGIIALLGILFFEVTSVASLITEVKGQTHSSEHSSQ
                     SFKQSEQSTIKSQSTETANVKEAIQESAE"
BASE COUNT        11908 a         6213 c         7114 g        10141 t
ORIGIN      
        1 aataaaatct ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa
       61 agccccaaac tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa
      121 aggattattt gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa
      181 taaaatccaa cgtatatgtc aaaaacattt tgtttctaga ctcaatagca acaacgagac
      241 aacctcaacc acatcctcga ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc
      301 aaaagcagca ggggagaaag cttattcaga aacaggtaga agccagacat ttagtactgt
      361 tgcggccact gcgaatttca acggacatac cataccagat ggatatacag tgatatatgc
      421 caaagatgga gtcattgttg cggttaacgg atcgatgagt gcggataaaa ctgcgttaca
      481 aggcgtaacc aaagcaatcg cttttattga taaccgtcca gatatccata atctgtttat
      541 tggctccaca gtaaatggta ctactatcac attcccaaaa agtatctata tgggggtggc
      601 taccgctgga aatacgatcg attcagttac acccaatgcg gataaaagtt attcttttac
      661 accctcaaca tggggagggc ttcctgccgg aggaggtatg agtacttttt caatcggtat
      721 atagttagca aatgatattc cattcacagg gtactcgatc aaaaatgggc agatcgttga
      781 tggtaaggga aatgtttcag ttttcccaac ctatcaagca gcgacggttc aggtcaatgc
      841 gaatcggaca ggaacttttt cttatacagt caatggtgga gcagcaaaaa tcggaacgta
      901 cgggactaca ttatctggaa tccatgtggg tgacaaagtg gttatcactc caacagcagt
      961 aacgggctat tcttttacac aaacaaacgg ttctctcaca gctctagcaa gtaacgtcaa
     1021 tacggtgacc tatacaggta aacaagcacc aatcactatt aagtaccaag actattttgg
     1081 acagacgatt gctcctccta aaatgatgaa tattacttac ggaagtgccg cacaagacct
     1141 gacgactaat gcgcctacta tcaacggata tacctttaca gctgtttcag cgacagaaac
     1201 aaagaaccaa tcagcaatga gtgtttcagc tagtttagat acaaatggaa atgtaatcgt
     1261 aaaggacgca aatggaaaac agatttctga agtcatccta tattataaga caaatgcaac
     1321 agtatcaatc aatgcaaatg gcagtaagta ctatgatggt ctttctgttg taccgatcgt
     1381 taaatataca tttaatgata aaacagaatc aacatccctt ttaaacaatg atctcagtat
     1441 tgatcaaatc acatggaatc caactgattt caaagcgatg aatgaaaagg gagttgaagt
     1501 aactgagcct actgaaattg gaacctatac gtcttggcaa ttgacaaaaa gcggactagc
     1561 taaactagct gccagaacaa actatttatt tactatcgtg cagacatctg atgtctatac
     1621 gattaagcaa atcagcggtg cggtagagct cggtgacagt aagacatatg atggaaaagc
     1681 aggtgtcccg tcaatccatg tcaagctagc agagggtgtc actagttcgc ttactccagt
     1741 acaagtggca cttagcagta tggattacac agtagatgca caatcagcaa aaaatatggt
     1801 taatgctggc agctatacaa ttaaactaac gaatagcgga atagacaaag tcaaagcagc
     1861 aaatcccaag ttgtcattta ctgatatagc caacacatca ggaacttata ccatctataa
     1921 agctaacgca gtgattactg tcgatgacgc tgcctttaat tatgatgccc aaagtcactc
     1981 gattccggtt ggaaatgtcc acgttaccgg tgttcttagg cgaatcattg gattataccc
     2041 taacagttaa cagccggaca gatattggat cacaaatagt aacgattagt ttaggcgaag
     2101 aaactgtaaa caataattat acgattaccg caacagatac cgctaagctg acgatcaatc
     2161 cgactctctt gcttccttcg acaggcggga taggtctact gccctttgtc tttttaggaa
     2221 tgatctttat tttttctggc ttcttttatt ttatccatcg taagaaaaaa gcaggtgaac
     2281 agcgataaaa aaaacaaaaa atgtacgtgt ggatatattt gctgttctct tttttatcct
     2341 actggtcttt tttataggtt ctccgactgt gtcagctaga gaatcaccta ttacatttac
     2401 tttgcataat cgaatgttcg aaagcgataa gatgccaagt atcagtcaaa acgacggaat
     2461 aaatatccca gataactttc aggaagcaaa ggggttgaac ggcgttactt tcgctatata
     2521 tgatgtctcg gatgaatttt acaagctgcg gtcagaagga agtagtgtgg aagacgcaca
     2581 aagaaaacta gcacaaaaaa gtgatagtga aaaaatactg gcagaaaatg tgacaaaaac
     2641 agtagacggg gaagagggca ttgcttcttt tagtgcatcg gacaaagacg agcaaggtcg
     2701 tgacgctgtt taccgatttg cggaaataaa gacttcagat caagtaaaag aaaaatcagc
     2761 accatttgtc gttgtgcttc ctgtgacaaa ttcatctaat cataaattga cgaccatcca
     2821 cttgtatcca aagagtgaac aaaaaatacc tgctgcactc acattaacaa aaacagtaga
     2881 gaataagcag acagattttg cagatgggga taaagtccct tatctcatca ccgtaacaat
     2941 tcctgagaac atcaacgaga taggaaccta tacgatcaaa gatacagctg atcctcagtt
     3001 atggctagaa ccagaaacga tcgatttttt gattggtgga gacaagattc atacatttca
     3061 cacacaaaaa acaaaacatg gttttgtctt atcagattct ggaaaagatc ttagttcgtt
     3121 tgctgggaaa aaattaacga tccgttacca aatgacattg aaagaaaaca gtgaaaaaac
     3181 gacatttgat aacgatgtac gtttgacgac tgacaatcaa acagtagaaa cacatcttgc
     3241 catccaaaca ggaggcaaac aatttgtcaa agtggatcgg caaacgaaac aaaaactagc
     3301 agatgctcaa tttctagtca aaaaaaatga ccaatactta gcgcaagaaa acgggataaa
     3361 tcattgggta gccaaagatg attcaagagc aacgattttt acttccagta aagatggaac
     3421 attttctgtc catggattgt cttttggaag ttacgaacta gtggagatta aaccgcctaa
     3481 aggatatata ttgagtcaat caaccattct ctttgaagtt gaaaaaagca gttatgatcc
     3541 gcttcatcct ataccattgg aaatcatcaa cgaaccaaaa gcaaatccga cgaataatgg
     3601 aaaaacgcca gttcagatgc agacaaatgg tccatcaaat gaaaaaaatg gaggaagctt
     3661 tccaaaaaca agcgaagaga tgcttggagg tttctcaatt ttagggttgt tgctagtcct
     3721 ttcaacaggg acagcttggt tttataaaaa acaacaaaaa ggaaacaata ggagggaaat
     3781 aaaatgaaaa acatcaaacc aaaaatatgg acgtttttag gaactgcaga ggtttctgca
     3841 gccgtatcac ctacaccaga aaatgttacg gttaatttgc ataaattgaa attcacttca
     3901 gcaccagaaa atcaaataaa caacggaacg gaattgacat ttccgaatag cgaaccgttg
     3961 aacggcgttg aattcaacga atatgacatc actgctacgt actatccaag taaagacaca
     4021 gcagttccag cagacgctac acccttcgca agtgtaacga cttctggtga aggattagct
     4081 aacctgactc ttccaggtaa atctgatggg aaagatgctg tatacgtctt tgtagaaaca
     4141 ccaaagcccg gtgtcgaaac gtcgccaaat atcgtattaa gcttaccctt ttataacccg
     4201 aattcatcag accctgacaa acctttagac acgattcacc tatatgctaa aaatgtcatc
     4261 aaagaaagca atatcaaaat ccagaaagct gggaactttt caggggtaga taagttagca
     4321 ggggcgcaat tccagatcaa aaattcagag aatcaattcg tgaccggttt tgatgaaaac
     4381 actggtgtcg ctctctatga tcctgctgag tcagcgttga cattcacaac tgatactgaa
     4441 ggatccatca gtgtcaatgg attaatagac ggcaattata ctttagttga aacaaaagca
     4501 ccagatggtt atcaattaac cgatccacaa aaagaaacta cttttatagt aaaaaacgga
     4561 aaattgcaac aaacaaccgg tatcatccat ggcgcagatg aagccaatct tgggaataca
     4621 aacggtgtag tcaatcggga aaatacaact acggataaca cgattactgt agaaaatgtc
     4681 caaaactacg gtgatttcca cttcatcaaa caagatgtga acacgcaaga aaaattagcc
     4741 gatgcagaat tttctattct aagagcaaaa gatccaactg ttaaattaat gaaaaaaata
     4801 gcaaaccttg cctcaggaga atacccatat gcttggtcag atgaagtcac aggttctgac
     4861 tgggaagaag tgaaattgat ttctgattca gaagggaaat tttcaatcga agggctgaaa
     4921 caaggggcat actgccttca agaaacaaaa gcaccagaag gctacgcatt acctaagaat
     4981 gaagctgcgt acactgaatt tacagtagat gatgatccaa caacttctga tgaaacaacg
     5041 atcgataacc aacctaaagg aatcttgcct tcaactggtg gtatggggat catcgtattc
     5101 atcttggtag gtactgcttt agttggagga gcagtgatct acttcaaaaa acgccatcaa
     5161 gaaacagaag cttaaactat tggaaacaaa aatcatacga taaataaacg acagtgattc
     5221 aaatggagag aaaggttgtg acagaagtgg tcggtctctt ggtattcgct caacaaacgg
     5281 caaaatagga ggaactattt ttccgtttgc ttcgctaata ctcaggtcaa ggcgctgatt
     5341 tcacaacctc ttcatataca tattagaaaa taggtggaca tgatgaataa aagcagaata
     5401 agaatactgg acatcttcat ggcaatcatt ttagtcgctg ggatcgggat cttttcttat
     5461 ccttttgtag aagattcact gaatgatttt cttgcccagc aaatgatcat ccattatcaa
     5521 aagcaagcat cgaataagaa ttcagaggaa ataaaaaaac agcaggaaaa aatgacaaag
     5581 aaaaaccaac agttggcaga gcaaaacgta tcaccaggga taggcagttt caaccaagca
     5641 gttgatgcaa aagcattaaa agatttacct agtaatgcct tttttatgga acatatgctt
     5701 ggagtcatcg aaatcccaaa aatcaatgtc agcttaccta tattcgatca gacaacggaa
     5761 atatttttgc aaaaaggaac gtcattattg gaaggaagtt cttatccgac tggcgggaaa
     5821 aacacccatg ccgttctttc tgggcatcgt ggactcccag aagcaaaatt atttacagat
     5881 ttgcctaagt tgaaaaaagg agaccagttt ttcatccaga tcaatggaaa gacattggcg
     5941 tatcaagtag aaaaaatcca agtggtctta cctgatgaag tagattcttt gggtatccaa
     6001 aaaggcagag atctcgtgac tttgcttacc tgcactcctt atatggtcaa tacacatcga
     6061 ctgcttgtga cggggcatcg tattccttat caagcaaaag aggcaaaaaa agcgatccaa
     6121 ggaattgatc aatggaaaaa atggaaattt tttatttggt ttatcggtat actacttgga
     6181 tcaatcggct tagtttggct actcatcgct tatttagatt ctttggccat cgctaagcga
     6241 aactatcctt tatcttttta tgtaaagaac acaaacggac gaccaatcga aggaatggtc
     6301 ttttctgtaa aaacgctcaa cggcaaacac tatattacta gagaaaaagt tccatttgtg
     6361 aaagcttctg atgaatacgg actagtgaga ttttctgatt taaaaggcgg gaactatcgc
     6421 ttgcaacatg aggagctcct gcttaaaatc catgtaaaac ataaacattc gaaacagttt
     6481 tcaatgaaat tgaaaaaagg aagatataaa ctgcgaaaag aaaaagaggt gtactatttg
     6541 attgagaaag aatgaatgaa gatgtccaaa aaagcagagg gaacagacta aatgaaaaaa
     6601 gagagaagct gacgaaatgg acgtgtaaac tgctattttt ggtttactat ctggaacgcc
     6661 agcttctatt tttttatgtt tcagaagaat tttattatta tagtataact tttgaattag
     6721 tttccataac atttggagca aggggttaaa ccacgagctt gagcttcgct taaagtggca
     6781 ggggtatacg taccatttcc acatttatgt gtatggtatt ttgaaccggt cggtgtgaca
     6841 tagacagttt gttcattatt ttgctcagga gctgcggctt gtgcctgctg agaggcctgt
     6901 tcagctgctg cttgctgtcg cgcctgttct gcttgttgct gtgcgattgc ttcttgcctc
     6961 gcctgttctg cttgttgctg tgcgattgct tcttgttgcg cttgttctgc ttgttgtcgt
     7021 gcaatttctt cttgcttttt ccgttcttct tctgcttctt ttgctttgat tgtggaatca
     7081 attgcagcaa gacggcttgt caactcttgt ttgttttcag gtagcgcttg gatagctgct
     7141 gaagcagttg catagttttc ttttgtagga tttgcttcag cttgtgacaa agcagattcc
     7201 gcttctgtca cttttttttc tagtgcagct ttttccgctt tttgtttctc ttcttctttt
     7261 ttcttttcaa tttgttcttg ttctttcgat gaatctgccg tactttcgga gatcgtggta
     7321 ctgctgctgg aagcctgagc tgaacttgtg ctttgagtag tagacgtagt agcaccacac
     7381 ccactcaaca aacttcctgc gaccaaaaac aacgcaacta attttttcac taaatttcct
     7441 ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta cttacttgtt
     7501 ataataattg tgttatgaat agtgattgag gaaacttaat ctaatccctt gttgatacac
     7561 gggatttttt gctatagttc tgttattaag tatgcaataa taaaatatgt acgttatttt
     7621 attattagat tattgacatt tatataataa acgattaaat gattcatatc aatagaatac
     7681 ttaggaaaaa aagagaaata ataacaggtt tatgcactat tgacggtgtg acaatatgat
     7741 gtttgtatcc gtttttttct tgtatgtagt atagtaaagt agatacagga ggagaaaata
     7801 tgcagacttt tttaacaaaa gtttctcaaa ataaaattaa aatgattcat ttactgcttg
     7861 aaacagaacg ttggtgttca atagaagaac tacaaaacga attgaaggta tcaagtaagt
     7921 ctatcttgaa ttatctaaca gatttagaag aattgttcca acaatatcct gataaggtca
     7981 ttttgaagaa tgataataat cggcgctttt ctatggaaaa gcaggagaac ttcccaatct
     8041 acattattta tcttcatttc tacagaaaat catataatta tcatttgatt gaattcatgt
     8101 atcaacatcc tgagaaaaat ttggaagatt atgcagacgc tcaatatacg agtgtatcca
     8161 cagtttttcg ttatgctaag ttattagtaa agtactttga aatgacggac gtcaaagaat
     8221 agtcgccgcc agatagaagg aaattagcag ttgaaaaagc tgccctgcat caagtaatac
     8281 gaccagccaa acggaaacag tgtctcatgt ttttgatttg gctggtctta ttaccgcaga
     8341 tgcaagcttt tgccacacct ctttcttgtc atttacagac tcaactatca atgctgcaag
     8401 ctttccctta atgctgtaac accagtaaga gttgtcaaga tcagcagact gactaggtaa
     8461 ataccgctca aaaacaagat tacggcagtt aaactataat gatccatcaa gaaaccgatg
     8521 acgatagaag aaaaaccacc aacagctcgt ccaaggttca taatgaaatt attcgccgtc
     8581 gcgcggattt ctgttggata caaactacta atcactgcgc catatccacc atacatgcca
     8641 ttgataaagt agccgactat cacagcggct gctaccaaac tccactggct atgggctaac
     8701 acaagtagat agacagataa ggatgaacaa attaaaaaca aagaaaaggc tagtttaggc
     8761 cccaatttat ccatgattat gccaaaagtc atcatcccca aagacatgcc taaaatagta
     8821 ctgaccatcc acaatgaaga cccagaaacc gaaagcccta atcgattttg cataattgaa
     8881 ggcagccaat tcattaaccc aaaatagccg gcaatttgga cagttaccat gatactaagg
     8941 ccacatgttt gccaagcaag atgttttgat tcaaacagtc tgctaagttt ggttttcttt
     9001 tcggcagatt gtacagattt gaattccttt ggttcagtca agccgcgacg aatgaacaag
     9061 accatcaaca ccggcagtac acctacgaca tacaacatct tccaaccaaa cgttggaatg
     9121 atcacagcag ctaaaatcgc agccaaagcc gcaccgacct gcgcaccaat accagcaacc
     9181 gaagaagctc gtccaatttg tttttttgaa aaactttcgg aaatcaagga catacacgcc
     9241 ccatattctc ctcccgctcc tacacctgcg ataaagcgaa agagataaac caagtagatg
     9301 tttgaagcaa aatacatcaa taaagaagca atcgaaaaca gaaggaccgt ttgagaaaat
     9361 actttcacac gtccatattt atcagctatg atgccaaaaa aaatacctcc aatgagcatc
     9421 cccaaattag tgatcgttga aatgaaaccc gcttgcgtac cactaatatg aaaagaagta
     9481 atcattgaag ataatgaaaa agctaaaaac ataatatcca tattctctaa agcaattccc
     9541 gccgatgtcg ataataatac ctttcgctga tacgttgtca tttttatacc ccttttccta
     9601 aaatttccct aaattgttaa aagtatatcc aaaaaagaat aaaaaaacaa gactttttta
     9661 tttgaaataa tacacaaaca accgatatag ataatccagc ttagttttaa tagaaatgag
     9721 tattgacttg gagtacactt aaagttgtag aataaataaa atacataagt aaagtggcaa
     9781 gaaattaaga gaaagagtga tcagatgaat atcaaacaag tcagtgaaga aaagggcatt
     9841 tcggccgata cattgcgcta ttatgaacga atcgggttga ttccaccagt taatcggaca
     9901 aaaggcggaa tccgtgatta tacagaagaa gacctaaaat gggtcgactt tacgatctgt
     9961 atgcgaaatg ccggattatc gatcgagtct ttgattgaat atattcgcct atctagcgaa
    10021 ggagaagcaa ctgtatttga gcggcgtaag cttttgattg aagaaagtga acttttgacg
    10081 aaaaaaattg ctgaaatgca agaatgtttg gaacgtttac aaggaaaaat caagaaatat
    10141 gatcgtgtgt tatctagaaa taaaatggaa tgtatcaagt aaaaaaatca aggagcatcg
    10201 ctatggagtt acgcttacta aaatattttt ggacaattgc agaagaagga acaatctctc
    10261 aagccgcaga agttctacat cttacacaac caaccttaag ccgacaactg aaggagttag
    10321 aagaggaact aggaacagaa ctgtttgtac gcgaaaaccg caaaatgatc ttgacagaag
    10381 caggttattt tctaaaaaag ccgagcagaa gaaattttag atttaacgaa tcaaaccact
    10441 caagaattcg agaacagaaa aaaacaatta ttcagcggcc aatttacgat cggctgtgtg
    10501 gaagctgata attcgaatac attggcacta ttactagaaa agttgatccg tgattatccc
    10561 agcgttcaat ttcatatcca cagcggaaca agtgatgata tcacggagtg actagaaaaa
    10621 agcctgttag atttagcgat tttgctagat ccagtagata cagagaaata cgaaacgatt
    10681 ttgttaccaa gaaaggaaaa atggggattg ctcattttag aaaattctgt tttagcaaaa
    10741 aaagacgtga taacaaaaga agactttgcg aatgtacctc ttttgatctc aaagcgtcca
    10801 gaaatacagc gaatgtttgc tgactggtta ggcataaaaa aggagcagtt gctgatcaca
    10861 ggaacgtaca atttgatttt caatgtattt gcgctaattg agaatcaggt aggtgctgct
    10921 ttagtcatag aaggtgctgt aagtcatcat tcaaagcgag tgacattcgt tccgcttgac
    10981 ccaatgattg aaacgaactg catgctagtc tggaaaaaga atcggatttt atcatctgta
    11041 gtcaatgaat tgattcaccg tttcaaagaa gcttttgaac agtaataagt atctctaatc
    11101 gaagaaattt caatacaaaa gcgagacgta tgacagtctt gcttttgtat tttttcattt
    11161 ttaaatcaaa aaaaataaag agatttacat aacttgttaa ataagcgctt acaaacataa
    11221 gaagaacgga aatgaatcag ctataataac cgttgttctg gattgtaatc attacaaaga
    11281 aatgaagatt ataactttgg aggtgttcga aaagaaaaat gggaggagct ttcatgaaaa
    11341 gatttagaaa atgtttgaca ttggtttttg tgttgagctg cttgatacaa ctttctgctt
    11401 tcaagcaagt agtgtttgct gcaagtgaaa aaagtagtgt aggtatcact tttgaaggaa
    11461 gtgttccgca aagctcaacc gatccatccg taccatcctc gcaagatgcc gaacaatcgg
    11521 caaatcaatc cagacaaaag aaatacccaa aaaccaacga gattcagcaa tcatttatta
    11581 cttggggtgg aatatttctt ttagtaatca gtttgttatt aatattttgg aaaagaaaga
    11641 ggaatagaga atgaaaaaaa caattttagc tgcagcatta attagtacaa cacttttcgc
    11701 aggaacatta gctgtacatg cagatggcct aaatgggaaa agtactgcaa agatcggttt
    11761 gacggcacaa gatccagatc atcctgtagg tccaattgat ccaatcgacc ctgatgataa
    11821 tcctccttca aatgatccaa ctggaaatac tggggacttg cgtatcgatt atatgtcaaa
    11881 aatcgatttt gggaaacaaa cgatttcagg aaaaaccgaa acatatactg ctgaaaaacc
    11941 agcgttaaca accgaagtcc aagtttcaga tctacgagga actggtgctg gctggaattt
    12001 acaagttagt tacgatacag aaaaaccagg atttacttca gaagataaaa cattgaatgg
    12061 tgcagaacta acattaccag tgggtacacc agaaaccgtg gaagacaata gttctgcagc
    12121 tcctgctaca agcaaagtag tagtgaataa tgatgcgcaa aacatcatgt cagcagctgc
    12181 taatactggt ttagggacat gggcagatga aatggataca gcaaatgttt cgcttaaagt
    12241 cccatcaggt aacttagtgg gtgattacac agctacactt gtctggactt tgtcagacgc
    12301 accagcttaa ttaaacattt attacgaacg aataaggaga gaataacaac gatgaaaaaa
    12361 caagtattct acacattagc agcgacagta ttgctatcaa gctcattatt aagcacgttg
    12421 aacgtacaag cagatgaaac agagccaacc aaagattctg caacttctac agcaaagatc
    12481 acgtttacag gagataactc tgatccagta aacccggtag atccagatga tcctgacaat
    12541 ccagtgaacc cagtagatcc ggacgaccca gataaccacg gtactggaga taacggtcct
    12601 ttgtcaatcg actatgtctc aaatatcaca tttggcacaa aagatatctc aacgactagc
    12661 caagtataca acgctgaaaa tgcagagcca catgttcaga tcactgataa acgtggagta
    12721 gaaggcggat ggacattaac ggctgcagct acaaaatttg ttgctagtga caattcagag
    12781 ttaaaaggtg cagaattatt atttaaaaac ggagtagcag aaacaacttc tgataataca
    12841 agtaaagcac ctgtaacaag tgatctgatc tttgacaaca ctgaagccaa aacagtcatg
    12901 acagccgata gtttaaaagg tcaaggaaca tggttggatg tctttaaagg aacagaagga
    12961 aacaatacaa atgttcaatt atctgtccct tcaggatctg cgcaagcaaa agaatacaca
    13021 tcaacgatca cttggacact cacagcaggc ccaactgctt agaatggaga gaaaaatgaa
    13081 caaacttcaa tcattatttg ttgctgctct tagtttatta agtattgtag tgatgccatt
    13141 gacgacatac gctgattcaa atgaatcagc gtatagcgtc aatgcaaata ttcccgactt
    13201 tcaaaaaaat aaaaacgttt cttactttga tttgcaatta gatccaaatc aaaaaaaaga
    13261 gatttcaatc catttagcta actcagcaaa agaaaaagcg atttttaacg tagatgttaa
    13321 taatgctgcc acaaacagta acggggtcat tgactacgga caaacagatt tgaagaaaga
    13381 ttcgtctgct aaatatgtat tgaatcagct agttactcct aataatcaag aaattgaatt
    13441 acaagctggt gaatccaaag atgtgacttt ccaagttcaa atgccaaaag atcgattcaa
    13501 aggaatcatt cttggtggaa tacacatcag taaaaaaggt aatgtgagtg aaaaagtaga
    13561 cggaacagca atccgtaata aatatgcgta cgtgatcggt gtgaaactgc aaaacgatac
    13621 agatgccgtc acgcccgatt tgaaactgaa ctcagcaaaa gctggtctgc aaaattccta
    13681 tacgactgtg tttgctaatg tgcaaaatcc aacacctgga atcatcagca aagttaattt
    13741 tgatgcgaaa atcacgaaaa aagggtcaaa agatgttctt tatgaaacaa aaaaggagaa
    13801 tatgtcgatt gcccctaata cgaactttga ctttccaatc agtttgaata aggagaagat
    13861 cgaagctggt gattatacgg tgacgatcga tgccaacgaa ggcaatacag ataaaacatg
    13921 gcatctgaca actgatttta cgatccagtc agaaaaagcc aaaaaaatca acaaagaagc
    13981 agtgacagaa gacaaaggaa tctcatatct cccagctatc atcacagtcg gaatcttgtt
    14041 attattgatc atcgcatttt taagttacaa attacttaaa caaaaaaagt aggtgagtct
    14101 ttcaagtgaa attgaatcag ccactaaata aattatatgt gttaggtttg ctttttatcg
    14161 gcgcattcag cgtgattttt caaagtactg aagtatcggc agaggcaaat tacccagaga
    14221 ttccagaggc aacgattttt gatggatatg tcaaagcatc gatttcttat gacaaggacc
    14281 attcacctaa ctatactgca gttggagata cagtaactgt tacaacaact ttagagaagt
    14341 tagatgatgg tgttttacca gataaatata aacaatcatc agttgttatt ttattttctg
    14401 atgaaacaca aggtcttcaa ttgaaaggag aaccagaatt caaatatgag ctaggtggtt
    14461 tggaagatac ggggaaattc gtcgatccaa cgaaggattt gcaaactaat gtgatctcag
    14521 tttttaattt ttcaacaaaa atcctataca atgatacagc gacatatgat ggaagaatga
    14581 agcaagtccc gatatcgagc acagagctag atgatggtcc atatgacgat acttattcgt
    14641 tatataaatt gttggtggcc aaaggaagta aagtcacttt gtcttataaa gcaacagtta
    14701 cggaagaagc gctgaaaaac aaacaattct cgctccatac agccatatat gatccaacaa
    14761 ttggcggtgg agattttgac tgggaccatt ttctaactac atctatgcca agtatccaaa
    14821 atttagggat ctcatttgac gaagctacta aagacaaaaa aatcgatata aaagacgatt
    14881 cttcttatca aactacttta accggttctt gggctggtgc aaaggacgat atccatcctg
    14941 tattgacgat tgatggtaaa gaagtgccta ttccagatgg tagttttaaa gaagatggta
    15001 catttgaaat cccagttgac ttgaagaata tagggaatat tggaacaaat aatgtccata
    15061 tcaaaatttc tgatagtaat ggacaagtcg cggaagacga tgcggctatt acacttgtcc
    15121 aaacattggt accaccacaa atcaaactct ctgattcttt agcaaatgcc tccatcagtg
    15181 tgtttccaat agatcagtca ttgacgatcg atggggaatg gaaagataaa gacagtaatg
    15241 ctgtttcttt attttataag ctgaatgggc aagaaagagt cttgcaagac aatgtcagca
    15301 atacagcaaa agactcatgg cttcctttct cacaagagat ttctctcgct gatcttaaca
    15361 ctggtgataa tcaagtagaa gtttatgcca aagatgcaga aggccagtta tctaatcatg
    15421 aaagttttaa aatcatattg aatcaaggaa acgtcagctt taaatcgatc aatccggaga
    15481 tccaattcaa taacatggtt atttccggta ccactgttta ttcttcacca caagcaactg
    15541 ttggggtagt tgtagaagat acgacaggaa ttccgaaaga ttggcagctt aaagtcaaac
    15601 agaccacatc tttttctgac ggaacaagag atttgcctgc gcaattgacc tatctgaatg
    15661 gggaagaaaa gctgtcaatc aatgatagct cagcagtgga tttgccagtc gttcaaaaca
    15721 atgctacgga ttattctctg caacaagatg cgaaccatca attccggtta gctgtttcac
    15781 caggcgctta tactggtgaa tatcagtcac agttggaatg gacgatcgag agagcaccat
    15841 aaaaaataag aaggggaaac gtggctgttt ttccttctta tttttgttat gatagaggcg
    15901 aaagggtgga gttagttgat ggagtcaatc atgtttgttc tcattgctta tctaaatttg
    15961 tttatttgga tgagtctgtt gtttaaaggg gcgtcaaaat ggcgattcct cttgttttat
    16021 ttggggctaa tgatagctaa tatgtttttt gttccataca cattcgtttt cttcgcaatc
    16081 gcttatgctg caatgtgttg gtttttatat cttttggtag aaaatatttt tttatcgatc
    16141 ctgttacaaa attttttatt gaatgtggta ggtatttcct ttttcttagc aatcgatatt
    16201 ccaaggtatt tgggatttta tactagctat ttgaatcttt cagtagaatt agtactagca
    16261 actagtttat ttttgctgat cagaaaagca gatcaaaagt atgatatatt cgattatttt
    16321 agtggctata cgaaaaaact gaaaggcttg acgattttat ctgttgtgat ttatgtgctg
    16381 atttacatct ttcatttagg tattagtttt tactcgttgg attatatcct tacttcgatc
    16441 atcacgcttt tatatagtat tttttatact gcttttttta ttgtctttat tatatataag
    16501 aaaaagttcc aagtgatcga actttatttg aaagacaatc aggagacaaa agaatactac
    16561 gagaaattag atgatttccg tcatgattat ttgaattata tttctgcgtt ggaatacagc
    16621 ttgatgaatg acagtcagga gaattctatt aaactattga cccatttaaa ggattattct
    16681 ttagaagcga tcgatgatgc gagacacaat cccttgaagc gaatcagtga tccggcagta
    16741 cgtgggcttt tttatcattt tatggaaaga gccaatcagt ctggcatttc ttatgatatc
    16801 caagttctga atatcatcaa taatatcaag gcagattata tcgatgttct tcgtctgtta
    16861 tctattgcac tgaataatgc cattgagcat tgtgatgaaa caagaaaaga agagccaatc
    16921 agtattacga tcaatcaaac aaaggagcgg ttttatttca aaatcgagaa tcctgctgat
    16981 atgtcctcaa caagcttagc taaattgatg aaaagaggaa gctcaacgaa aaaaaatggg
    17041 ggactcggtc tttataactt ttcgaaaatc acggataaat atctgaatgc caactatgga
    17101 atcaaatatt taaagcgtac gaaaatgttt gtcttggaat tgacgattta tgagaagtca
    17161 atctgtaaaa ctgtatgaat agctaacaaa gtggaataag gaagttaaaa acgggcagga
    17221 cagacatgtc ctacccgttt gtttgtttta gttggtcaat atggcgtaac aattttttta
    17281 cactagaaga acttaaaaga agtgtatctt tatttttgaa atggacttcc atcatttgtt
    17341 tatctacttc tttgatattg aatgggttga tagaaaaact tttcagatca ttaaagaaat
    17401 gatagtcttg gaatttttgt ttgatatttt tcattttatc atgaataacg aagttctcat
    17461 ttgttccata aaattccaag gtggaacgag aaccattgat cgtcgagata tagttgactt
    17521 catctggcgc aatcaaaaag acctttgtgg cgccttgcag ttcaatgata gaggaagatt
    17581 gtttttgagc ttccaatttt tctaaaagta gctgtttcaa tgattgtgca acttcttcta
    17641 agtttgattt atcaagatag cgatctgggc gaatctgctg attaatgatt tctaatccgc
    17701 gtgtattatc gtttgataga aaaataatcg agcattctgg attcttgttc cgtatttttt
    17761 tagcaaaatc aatgccacta tgcaatgtct ttaaatcaat gtcgataata aacaaatcag
    17821 aagaagaaaa gaccagtgta tctaaacgct tataaatcgt ttccaactcg ctgttcgggt
    17881 atatttgaag agagagggca ctcaactctt tatgttttag taattctttc ttcaataatt
    17941 cctgatgaaa caagttatct tcaatgtagt agattttcat ggttcgattc ccctaagaca
    18001 gatttcatta cgattagtat aacagatttt ttctataaat ttcatcatat aaatgatatt
    18061 tgttttttta ttaatcagaa aatatacact tttatcgttt taatggacgg aataagcata
    18121 atgttgatcg cggtattctc ttggcgtaag ggaagttgtt tttttaaaga cctgaataaa
    18181 atggctttga cttgcaaatg aaagcaatac gctgatttct ttataactct tgtccgtaaa
    18241 aagcaacaaa tgtttacttt cattgatctt ccgttcttgg atgtaggagt ggatcgtttg
    18301 gcccatttct tttttgaata aagcagaaag atgtgttttg tgcttgtcaa atgtctcagc
    18361 tagctcgtca agcgttacac ggtcgtaaat atggctgtct atataattga ttagttgctt
    18421 gactagcatg gatttatcgg ctagaggttg ctgatggatc agttgcatga aacgataagt
    18481 cgcttctttc atatatgtgt gccattcact agaagaatgg atacggaaca agccttggat
    18541 caaagcatca gataaactga aggcctgatt aatcgggact ccttcatcga tactaatacg
    18601 cgtcaggatg gcaacgaaag cagagaactt cagcttcaaa ccttctaatt cattttcgga
    18661 caattcgaca tgatacgttt cttgcatctt tttgaagagc caatcaatct tttctggctg
    18721 attccgtttg atatactccg tgtatcgaag ctcgaattga taagaatcaa aagtggcttc
    18781 ttgcatccgg cggtcgtaca aactgtcctc taaccgttga tccgtggtag atcgctgctt
    18841 gatttctttt tggtaaaccc agtgcaattc ctcttttttc aagagttcgt gtgttagaaa
    18901 agcataggta agctggacga attcttcaaa agctgcagtt gtttctttcg tatagtgaat
    18961 accatagtga tagctatgtc ctttgaaaag gaaagtatca ggagattctc cgatcatacc
    19021 gcaagggccg attaataagg aatagggctt gctgtctgta ataaaatcca gtttagcaaa
    19081 cataaaagga aagtgattaa ataaacatac ggattgattc tctttaggcg aaatgaattt
    19141 ttccatgata ggctgtatct cccatgaaat cttcgggtgg caaacttcgc cgcttgaatc
    19201 gaataaaacc atggggatca gatatttttt gtataacaaa tccattattt tagaaatcgc
    19261 tgtctgtttc aattattttc ctcctgtcta tcctttttct tccattgtat cgcaaaaata
    19321 acatgttgaa tattaatttt ttcaggtatc tctcttctat ttttatataa agtcttcttt
    19381 tgttgtataa caaagaaaat tttatcgtta taattaaatg tgtaaaaacg gttacaaaaa
    19441 aaggagaaaa taatggatga aaaacaatta aagcaattag tcgattcctt gacaataaaa
    19501 gaaaaaatcg cacaaactgt tcagttgaac ggcgacttat ttactgatag cgatgtgatg
    19561 aataccggac caacaaagga attaggcttt cctgatgatt ttgatttaag tcagattggt
    19621 tccatttata atataaatga acctgaaaaa ctaaaaaaaa tccaaaaaaa aataatggaa
    19681 gaaaggaata aaaaaattcc gctcctcttt atgtcagatg ttatttatgg tttccgaacc
    19741 atttttccta ttccgttagc acaagctgga tcgtatgatt ttgacttgat tcaaaaagcc
    19801 gcacgagaaa cagcgaaaga gagttattta agcggactgc atgttctatt ttcacctatg
    19861 cttgatttag taagagatcc tcgctgggga cgggtgatgg agtctccggg agaagatgtg
    19921 tatactgcaa aagaatttgc taaacacgct gtccaagggt atcaaggaga caatctccag
    19981 ttgattgaca aataccgagt gggcgcatgt atcaagcatt ttgcaggata cggggctcca
    20041 gaagctggga gggaatacaa tacggtcgat atgtccaacc aacgtctctt caatgaatat
    20101 ctcgctcctt atcaagcggc agttgaagcc aattgtttgc tagtcatgac cgcgttcaat
    20161 gttttgaatg gcgtgccttc tactggtaat cgctggctta atcgggatat tcttcgaaaa
    20221 aggttcggct ttaacggcgt actggtatct gattatgcgg ccattgaaga gttgcaagta
    20281 catggctatg cgaaagatca agcagattca gctaaaaaag cattagaagc cggtgtggac
    20341 ttcgatatga tgacttctgt ctatgccaat tctttagaaa aattagcaga agaaaataaa
    20401 gagatcttgc aattgttaga tgaagcagtg tggcggatct tagacttaaa gaacaaactt
    20461 ggattattcg aagaccctta tcgtgggctc gaagaagaga acacgggaga aatcttgacg
    20521 gaagaatcga aagaaatagc agttaagtta gtagaaaaga gttgtgtctt gctgaagaat
    20581 gaggatacgc ttccattaaa aaaacaccag aaaatcgcag ttatcggtcc ttatggcaaa
    20641 agtcgtctga caattggatt ctgggcttct gtcagtggta aacctcaaga tagtattccg
    20701 ctaaaagatg gaatagaaca gcatttttca aaagaacagc tattatttgc aaaaggattc
    20761 aacctgtttg attcttatga accatttgga ccgttaaaag ctggaattga acaactgaat
    20821 ggaacaatcg aagacgaaga aacattattc caagaagcat tggaaactgc tgaaaaatct
    20881 gacgtgattt tactgacgat cggagaacaa tttttagaaa gtggagaagg agcttctaaa
    20941 actaaccttc gattgcctga aaagcaaaaa cggttgatca aagcgttagc agcattgggc
    21001 aaaccaatcg tggggatttt atataccgga cgtccgcttg tcctaacgga gatcgaacct
    21061 tatttctcta gtttgctgct cgtttggtat cctggaacga tgggaggcat cggtattgcc
    21121 aatcttcttg caggaaaagc agcgccaagt gctcgactca gcatgacttt cccacggagt
    21181 gagggacaga ttccaatcta ttatgcacat gcttcaactg gccgaccttt agaaacaagt
    21241 acgcattcca ctcgctttgt gtccaagtat attgatgaat ccaatgagcc actgtttgct
    21301 tttggcactg gtaaaacgta tggaaaagtc gaagcaagct ggcaggagat acaggatcaa
    21361 gaagataagt tgcaacttgt ttatgaagta atcaatcatt cagcaaaacc aatagaaaca
    21421 gttatccata tttatgtaaa ggacaagaca gcttctattg tgcaaccagt tagacggttg
    21481 attgctagtc aagtcgtttc tatacatgca gatgagaaaa aatcgattaa ctatacatta
    21541 tcaaaagaag cacttatgtt ttataacaat caaggggaaa aaatttggga gtctggcgaa
    21601 tttacttttt ctctctcgac agtagattca gaagatgacc gtacaattca attataaaaa
    21661 aggagaagaa agatggaaaa attagaacag tttttaagta gcagactatt gccgatgtcg
    21721 gagaaattac aaaagaacaa agtgttagcc gcattgatgg aagggtttat ccgaacaagt
    21781 ccaattacat tagggattgc cttcattaca attattggta atttcccatt tcctggctgg
    21841 atcgattttt tgaaaaatgt ggggattttt cctcatgtag aagcaatcac taatggtgct
    21901 acaggtgttt tctcgttgta tgtcgtatat agtttagctt tctcttatgc aaaacaatta
    21961 gggaccaatg aacggaatgc ctcgatcatc tctttggcaa gtttcattat gctgatgccg
    22021 caatccgtca tgacaactgt cacacaagat gggaaagcag ttgaacaagc tgtcggtgcg
    22081 ttacgattgg attatctggg cggacaagga ttatttatcg gtatgcttgt cgctttgctc
    22141 attacccgct tctatgcctt cttagctaag aaaaatatta tgatcaaatt accggaaagc
    22201 gtgccgccaa tggtcacaca atccttgtca ccagtatttg tcgttacgat catttttgtt
    22261 gttgtatttg ctttgcgagt attgtttggc ttgacacaat caggaacgat tttccaattc
    22321 tttatcgacg taatcaatgc cccattgaac tctttagtag cgagtccgtt atcgatcatc
    22381 attatcatgg aattattagc tgttttatgg ttcttcggta tccacaatgc tgttttgcaa
    22441 ggacctttag gcgcaatcag tatgacgatg gtcgttggga acattgctgc ctttcaagaa
    22501 ggaaaagcat taccctattt agttccgtct gttatctaca tggggatgta cgcagctgga
    22561 ttcatgggat tcgtgacatt tttcatgatc aaaagcaaat cagccaaaat gcgccagtta
    22621 ggtaaattat cttttgttcc atccttattc aatgtgacag aaccattgat gtttgggatg
    22681 ccaatcatct tgaatccgat tttctttatt ccacaaatct ttacacaatt gattgcagga
    22741 ttcgttacat ggggattaac cactaccata ttgccgattt ctttgaatcc gactatgtcg
    22801 ctattacctt ggacaactcc tacatttgtg aagatgcctt tatcaggcgg attgaactac
    22861 acgatcctaa tggttatttg tatggcaatc ggtgtggtga tgtggtaccc attcattaag
    22921 atcgcagata agaaagaact agagttagaa gcggcgctgg aaaaagaaga agcagaaacg
    22981 aaagcagaaa aaatcatcaa tgcagaagtc gaagcataat gaaggggaaa agcatgtgga
    23041 cattaagtat cgatatcggt ggaacgtata tcaagtcagc tttaataatg gatacgcaaa
    23101 taagagaaaa aagacaaata gagacaccga aaacggataa ggacaatttt atccttgtcc
    23161 ttgtcgaatt gatctattct tatcagcaga tcgaacccat tgaatttgtc ggattttctg
    23221 ttcctggtgc ggtgaaagaa gcgtcaaccg tcttttttgg tggagcagtc gcatgcctga
    23281 atgaagtcaa tttaaagcaa gaaatagaaa aacatctgcc cgttcgtgtg tttgtagaaa
    23341 atgatgcgaa agctgctgta ttgggagaag caagtttcgg tcatttaaaa gggattgaaa
    23401 atggtgcagg aattattcta ggaacaggcg tcggcgttgg acttttgtta gatgggcagg
    23461 taagaaaagg accccattgt caagctggag aggttagttt cttgatacaa gatcgtggga
    23521 taaatggggc tgaatcattt gcagggatca atttgtcagc tgttcgatta gtaaaagaac
    23581 tggcaaaatt atttcagtgt gaaccagaag gacctcttgt atttgattat ctatatcaaa
    23641 aagaagatga acaagcacaa actttatatc gcacatactg caatcaagta gcgattcttt
    23701 gtttcaatat ccagtgtttg ctggatttgg acaaaataat tattggcggt ggaatcagta
    23761 aacaaaaaag gctgatcaga gatatccaga aaaactatga agccatcttt tctgtgtcgc
    23821 cgatgatcga acaaacgatt acgaaaatga cgatagaagc tgccgccttt gagtctgaag
    23881 caaatctgat cggtgcagcg aaaggagtac gattgaatga gatttccaac tgatttttta
    23941 tggggagctt ccactagtgc gtatcagaca gagggagctt gggatgcaga tggtaaagtg
    24001 ccgtctgttc aagatatgaa agaaatacct gacggaacga gtgatttcaa agtggcggta
    24061 gatcattacc atcgctttga agaagatgtt cgtttattta aagagctagg attaaaagct
    24121 tatcggttct cgatttcttg gagtagagtc atgtctaatg gaaaagttaa tccaaaaggg
    24181 ctggagtttt ataagaaact cattgcttta ttgaaggaaa atgagatcga gccgatcgtg
    24241 acggtgttcc attttgattt gccttatgaa atccatgaaa agggaggatg ggaaaataga
    24301 gcaacgatcc aagctttcgc tgattactgc cagctacttt ttgacacatt cggtcaagaa
    24361 gtaaactatt ggcaaacaat caatgaacaa aatgtcatgg ctttagctgg ttcgatcatt
    24421 ggtacaagta ataaatcaat gaaagaaaag tttcaagaaa accatcatat gctcgttgct
    24481 caagccctag tcacgaaaaa ttatcatgat gggaattatc caggaaagat cggacccgcg
    24541 ccgaacattg ccagtgtata tgctgcttct gataaaccag aagaccagtt ggctgcaatg
    24601 aatatgagtg ctttaagaaa ctggctgttt ttagatgctg cggtttatgg tgtctataac
    24661 cataatgcct ggcacatttt gtcaaaaatc aatgcctgtc ctgaagtaac agaagaagac
    24721 aaaaaaatca tgctagaagg tacatgcgat tatatcgctt ttaattatta caataccatg
    24781 actgtgtcag cctattatca aaaagaaaac aagatcgatc agcaatctgg ctttggtatc
    24841 cctgatttct ttcaagcaac aacgaaccca catttgccaa tgactgaatt cggctggcca
    24901 atcgatccag aagggttccg ttatacgtta aatgagattt attcaagata tcgtctgccg
    24961 ttattgataa ccgaaaacgg aataggagca aaagatcaat taacagaaga tggaaaaatc
    25021 catgacaagt atcgcatcga ctatttgaaa cagcatatcc agcaaatgta tctagcaatg
    25081 gaagatggtg tagaggtgat cggttattgt ccgtggagcg cgatcgattt gatcagcacc
    25141 catgaaggaa tcgaaaaaag atacggtttt atttatgttg atcgaacaga tgattctttg
    25201 aatactttgg atcgttacaa aaaggatagc ttttattggt atcaaaaatt gattgctgaa
    25261 aatggattaa acgaagagga gtaagcctat gcagatcaat acgatgacta ttgacaacga
    25321 agccaatacg actctaacgg tttatcttca tgaagaaaaa cccgaatttc accgtattga
    25381 caaacgtccg tttatactag tgattccagg aggagggtat ggattttgca gtgaaagaga
    25441 agcagaaccg attgcattga aatttgctgc tgaaggttat catgcgggtg tgctgcgcta
    25501 tcacgttgga gaacaccgtg atttccacaa agcactggcg gatgcagaaa aaagcatgga
    25561 tctgattgcc ttactagctg aaaaagcaaa aatcgatcaa tccaaagttg cagtgatcgg
    25621 tttttctgcc ggtgggcatt tagcagccgc tttgtctact ttgtccgatc gaaaaccaag
    25681 tgcttgtatc cttgcctatc cggctatctt aagctccttt gcagaagcga tgaagatcaa
    25741 ggctccttct ttagacatgt gtgtgacacc gcaaacgcca cctacttttt tgttttctac
    25801 attcgaagat aatgtggtgc ctatcgagaa ttctattttg tatttgagag cgttagaaga
    25861 aaacgatgta ccatttgaaa cacatatttt tcaaaaagga aagcacggat tatcattagg
    25921 aaataaatgg agtggaaata gtgaagagat ggtggaccca cgatttgcca agtggtttga
    25981 gctttgtatc gagtggctgg aaatcatttg gtcacaagcc gctgtagaaa ctacacaaaa
    26041 aatagaagac gtttggcaag taccgatcaa aaatttatac gccattgaag caaatcgttc
    26101 gatcttactt gaacatttgc catttttagc cgataaaagt aggtacaaaa tcattcgaag
    26161 tttgtccctc aaccagctgt gtgaattttc gccagagaaa tttacaaaaa caaagatcag
    26221 tcaatgggta aatgaattga cttggaatct ttgaactttt ccatcaaaga atcgatgatc
    26281 ggaggaaata aatgaaatat gaaataatca ataagaacaa tcggttctat tttctcatgg
    26341 atgaaaaacc ctgtcttctc ttagggggag aagtgcataa ttcagcagct tccacggcac
    26401 tttctatgga aaaaaatatt tggccatttg tgaacaatat tggattgaac accctgcttg
    26461 tacctgttta ttgggaaaac attgagtcaa ttccgggaat attcaacttt gatttgatag
    26521 aagctcagtt acaaaaagca gctgaaaata agatcaagct ggtttttctg tggttcggtt
    26581 tatggaaaaa cggtttatct acttatgttc ctccgtggat caagacagat actgcaaatt
    26641 attttagagt agtcaaccaa aagcaagagc agttaaattg tatctcaccg ttttgcgaga
    26701 aagcaattga agcagattgt cgcgcattat ctagcctatg tcaatttatc agtcagcatc
    26761 cgctgaaaga tcaagtatgc atgatgcaga tccaaaatga ggttggctgt cttgaaacgg
    26821 caagggatta ctctagaaaa gcacagcaaa tatatggatc cacacttcca gaaagaatca
    26881 gagaattata tcctcaagca aacagctggc aggaactttc agacgagaca ttcatggcct
    26941 atgcctttag tcatgcactg gaaaagatgg cattagaagc aaaaaaatgg ttagatatcc
    27001 cgttgttcac caatgcttgg ctgcataaag aaggaaaaag cgcgggcgac tatcctagcg
    27061 gtggtcctac aaaaaatgta ttaaacgtat gggaaaaaaa tgcacctagc ttaacggctt
    27121 ttgctccaga tatctatgaa gaaaattttc gggaaatcat tcaagattat caaatgattg
    27181 atcaacccct tttcatacca gaaacaagaa aagatctaag atatctatct aattgtttct
    27241 atgcatttgg gaaaggcgca cttttgtatt ctccatttgg aatcgaagat cttagaaaaa
    27301 gtacggaagc tataatagat gaggatcagg cttttttgaa agagttagga ttagaccaag
    27361 cggcttttga tccgacagga agtttgcctt attttactaa gtgttatcaa ataattaaag
    27421 aactaacccc gtttttaacg gatgaaacaa tcaagatcca ttcttttaaa aaggaaaagg
    27481 acatgacgta tcagtatcaa gatgaaaatt accgattttt gatcaaatat ttaagcaaag
    27541 acaagaaaga gcagctgaac actgctggcc tcttttttca attagaaaat gattattttt
    27601 ttgctggatg taactttgcg ctattccacg atgcagtaga tgaagagctg tgttcagaaa
    27661 ttttgaattt tgaagaagga tattttgaag gaaaaaaatg gatcacagag cgtgttttga
    27721 atggggatga gcgctatacg ccatatatcg gtaatatgcc taaaatcatc aaattctctt
    27781 tatataccta tccgaaaaaa gaacaggagg ttgtccatga atatcttgct gacgaacgaa
    27841 acctttaaac catctactag tatgtttgcc caagaaccgg agccactcga aaatctatct
    27901 gtttatcgaa atagtacagc cgcatttcag ttattgctgc atgatgggaa gaaaaaccat
    27961 tacagcttag acgatcaatt tacgatccca gatgcgattg aaatacctat ataccgaata
    28021 gaaatcaact cagaactgcc catcaagtcg aattttattg aatattattg cggaaaagac
    28081 gacattttct acggagataa actgttagaa gaaaaagcac atacttattc tggtgatcgc
    28141 tttactccta tttacgtaga aataccggtt gatggtcaac aaaaaaccgg cacctatcca
    28201 atagatatca cgatttatca agcaggaata acaacaaaag aaacagtcat cggacagaaa
    28261 cgattagaag taactgtaga agcatttact ttcagtgata aagtaactga ctccttccat
    28321 ttagacattt ggcagcaacc ttctaatctt tctcgtacat tccaagtgcc tctttggagt
    28381 aatcaacatt ttgaactgat tcagacaatg gcttcaactt tagctgaatt aggccaaaaa
    28441 accattacag tcattgcagg agaaatccct tggaaaggat ggttcaatta tattgtcaaa
    28501 gatttcccag ctaacttgta tgaatactca atggtcaaag tcctcaaaac aaaagaagga
    28561 aatatccagt gtgatttttc aattttggat aagtatttag cttgtttttt tgaagcaggg
    28621 atcgataaag aaatcgatgt gttcggcctt ttaggcgtct ggcagccacc cttcttccct
    28681 ttgaataaag cagtagaaca tccagaaaaa cttgtgatcc gttattatga tgaggaaata
    28741 gagcaaatgg cattcatcga aaaacaagaa gagctcagac aatatttcca gcaattgttt
    28801 acctatttta agaaaaagaa catttgggac aaagttcgtc tcatggcaga tgagccaaaa
    28861 gcacatgaaa tcgctacgtt taaagaagct gtcaagtgtc taaaagaaat tgaaccaaca
    28921 ttacagctga aagtggcatt tgacaaggaa ccagtcttac aagccttgat ttcagagatc
    28981 gactatccag tcacatcttt ttattgtact tgtcaaaatt atgaatggct gaaggaacag
    29041 tttcctggaa aaacacaata ctacatctgc aattatccag ataaaccaaa cacattcttg
    29101 cacagtccct tgacagaaag ccgcttacaa ggcgcattag cttattattt tggaacagat
    29161 ggtctgctga gatgggcata taactgctgg ccggaacatg caagagaaga tattcgttac
    29221 aataccagtt cacttccaat tggtgatttg tgcttggttt atccatctgt ctccggcagc
    29281 ctgctcttgt ctcttcgcta taaagaactg tatcgtggtg tagaagactt ttatctgcta
    29341 aaagaagcgg agaaaataaa caaaacaaaa acagaccagc tcctagctga ttttcttgga
    29401 gggaaaaagc cagaaaactg gatggaaaac agccatcagt ctgtgcagag attattcaat
    29461 cagtcttaca gtcaattcga tcagttaaga aagaaattga ttgcgatcat cacagaaact
    29521 tctatttaat taaataatag tgaataaaaa gcatgacctg catattgcag gtcatgcttt
    29581 ttatttttaa ccgagatagg aaagaatcca ccgtaaaaat atttccagca cagcgagttt
    29641 tgtaaatctt tgtttaaacc agtttagatc tagctagctg aaaagagaca aagaaccttg
    29701 ttcctgctta gataaacttt tgggatagag aaatcacgaa tttacatagt ttttacaatc
    29761 tttatttgaa ttaagaaaga tacaaattag aactttacaa atagagggta aactaaagat
    29821 gtagcaaata aataaaagga gctaagatat gtttgagttg aaaaaaatag aaaaaaacga
    29881 tggtgagtat agtattaata gcaactctaa tggtattagc aggttgtcgt tcgtccaatc
    29941 agacagtaag tggtaatcaa aaccaatcct ctgatacgat caaaattgct ttttacccta
    30001 atgaatctgc tgcagagttt gaaccggcaa gagaagcggt aaaagcggtg gttgaaaaag
    30061 ctacagataa gaaagttgaa ttagtgacaa gtacagatta caacgtggtc gtggaatcta
    30121 ttgcaaatgg acaagtagac ttagcttata tgggtgcaga aggctatatc gaagcaaaca
    30181 agaaaaatga gcaagtagaa gccatattga ccaattctgg gccaagcgga acgctggaag
    30241 atgcgttata ttacagcttt ttagcggtga aaccagaaaa cagcaaagag tatgaagaaa
    30301 caggtggcta tacgattaat ccaatcaaag acaaaacgat ttctttcgtc acgaatacct
    30361 caacttctgg ttttactgta ccgagcaaca aaatcgctga acattttgat ttaaagaata
    30421 cagatgatct tatcatggat ggtctattct ttgaaaaagt gttgtttggc gggtctcatc
    30481 aaggatccat gatcaattta ttaaaaggcg acgcggatgt agctgctttt atgaatatgc
    30541 cgcaatattt tgatgtgatc caaggagaag aaaacaaggc aggagtcatt tacgaagtaa
    30601 aaaaagatgc tcaaactcct tttgacacgg tacgagggga acaagcacaa gttattcttt
    30661 ccataccggt cttgaatgga ccaattgcag caaacaaaca gacgttgtct gaagcagata
    30721 ttgataagat catcaaagga atgactgctg atagtacagc tgaaaataaa gctatttttg
    30781 caccaaaaga tgctgatgtc ccaggtatgt tcacaaaaga aggaaatgag cggtatgtgg
    30841 aagtctcaga tgacttttat gatgagatca aagaaatgaa caaataagaa aagactggaa
    30901 aggaaagcca aatgctatta gaagtaaaaa acatcacgaa aacctatcca aacggaaatc
    30961 aagcattgca ggatatttca ttttcacttg aaaaaggaga atttgtttcg atcatcgggc
    31021 cgtctggggc gggaaaatca accatcttgc gtacgatcaa ccgattaatc gaagcagata
    31081 gtggtacgat tcgatttgag gatagagaaa tgaatgaact gaaaagaaaa gaactgcgaa
    31141 agaaaagaag acgtattggt atgatttttc agcattataa tcttgtcagt cgccttactg
    31201 tgatcgaaaa tgtattgcat ggctgtttgg gttataaaac aacactcagt ggagctgctg
    31261 gtctttatcg agaagaagaa aaagaacagg cttttgatct tatagaaaag gtggatcttt
    31321 cggcatttgc gtacactcga tgtgacgaat taagtggtgg acaaaagcag cgggtcggaa
    31381 tcgccagagc actcatgcaa aatccggcac tcatcttatg tgatgaacca atagcttctc
    31441 ttgatccaaa atctgctaaa acgatcatgg attacttaaa aagaataacc aatgaattgg
    31501 cgattacttg ccttgtcaac ttgcatcaag tggaaacagc catcgattat tccgatagga
    31561 tcattggagt gaatagcgga aagatcgtat ttgagggaag gccatacgag ttgacaaaag
    31621 aaaaaataga aacgatctat caatctaaaa aattattagt gtaaaggatg agagaatgga
    31681 aaaaatagag gcagaaaaat ttttcagaca acaaaaaaga tatcgcttgt tttatatttt
    31741 gctgattgct tttctttatt ttatttcagc acatggcgtt ggatttcggg caggagcagc
    31801 tttttctgct gtgccaaaaa gttttttctg gctattctcc aactttattc cgacaagtga
    31861 ttccctagcc tatttatcga tcatcatatc tagactcgtc cagacgatac tgatggcagt
    31921 gactgctacg acatcaggtg ctatttttgc cttgatagca gctgtgattg gatcaagaga
    31981 aatcggtttg aatcgtttca caaaagtgat cgctagaggc atggcgagtt ttttcagaaa
    32041 tgtccctatt gttgtatggg caataatcct gttgttaagt ttcaaacaaa acgagtttac
    32101 cggatatttg gcgattactt ttacgacatt tggttatttg acccgttcat tcatggaaac
    32161 aatcgatgaa gtatctggca gcgtcattga agcgatgatg gcatctggag cttcctattt
    32221 gcaaatcatt tggcaaggtg ttttgccgat gtgtttgaat cagctcctta gctggcttgt
    32281 ttttctaata gaaaataata ttcgtgatgc tacgctagtc gggatactga cagggactgg
    32341 tattggtttt ttatttgact tttattacaa aaaattccaa tacgatgtgg tcgggatgat
    32401 tacgctgact gtcattattg cagttatctc actggaggca atttcgaata cagtaaggag
    32461 gatgatccgc tgatgatgga ttatcagaca aatgaaacga atgccatatt taatccaacc
    32521 tcttattcaa aggtggcatt caggaaaaga aatcagagga taaaagttaa actatggcgt
    32581 caagaacgga tcattgtaag gctcatgctg ctacttttat gtttggtttc tgcatacact
    32641 gtgatcacaa tagattacgg gaatgtcaag ttaacagaag cagtttctgc ttttttttcc
    32701 aatatgttca atattttctt gcgtccgcag ttatctggaa gatttacgac gttttcttta
    32761 gtagaaagtc ttggtataac gattggtctc tctgttttaa caactgtgat tggtgctttt
    32821 cttgctttct ttcttagcct gttagcttcc cgaaacttaa gcagtggctt tctttccaat
    32881 acgataagag ggctgatgtc ttttattcga gcagttccca cgattttatg ggtcatgatt
    32941 ttttctattt tgatcggatt aggagcgaat gctgcaatta tcgggatgtc ttttcacagc
    33001 attgcttatt tgacaaaagt ttattctgaa agtattgaag aaatggagtc tggcatgatc
    33061 gaaagtttga aagcgtgtgg agcgagctgg tggcagattg tttttcaaag tgttctccct
    33121 cattgtttca cgacattgct ttcttggaca ttcattcggt ttgaaatcaa ttttacaaat
    33181 gcgattgcgg taggagctgc agctggtgca ggaggattag gctttcagtt gacgatggct
    33241 agtaactttt attttgattt tcgagaaatc ggcgtgatgg tctatatgat cttgacgatt
    33301 gctgtactgt tagaatttat ttctttgaaa ttaaaagaaa aatatattgt caaaacataa
    33361 aattttatag aaagaggaga acgggaatga aggcagactt acatgtgcat agctgctatt
    33421 cgaatggttt ttttcatcca aaagaacttt tgtctttagc gacagctcaa gaaataaacc
    33481 atttagcaat cgtggatcat catacattcg cagggataga agcagtaaaa gaagtattct
    33541 ttgagtcgcc tgttaatttg atcgaaggaa tcgaaattag tgcttttgac ttgaaaagac
    33601 aaaaacgtgt ccgcatattg ggatataatc tttcaaatcg gagctatatc aagcagttgt
    33661 gcagaccgtt acttcatttt caaaccaatc aaactttatt taagttaaaa aaactgcagg
    33721 cagaaggtta tcatctgaag gaagaggaac tttggaagga agcagagaag gcaactgcac
    33781 tttatccctg tcatttatat cgtttattga aaaaaagagg ctatggtaca cagatggacc
    33841 atacgtttta tcaaaaactt atgacagcag aagaagtttc tgataatcga aaaaaaatag
    33901 atgtatatga tgccatagag gcaatcaaat acggtggtgg gttgactgtt ctagcctgtc
    33961 caggaaaatt tgataactat gatttgatcc cagaactcgc ttcattcggg attgatggtt
    34021 tggagaagta tcatccagct catcgtataa gagaccttaa gatccttaca gatttcatca
    34081 aaacctatcg tttttcttgt tttggcggtt ctgattttca tggaataaga gggactacag
    34141 gatttggcag gaccttgatc acagatgcgc gagaatttcc cgatcaacta ctctataatt
    34201 gaataaacta tctgtttcag aaaaatgaaa aaatgattgc agcaactata gataatgata
    34261 taataacctc aattacgtat gaggggattg tgaacgatga tcaacttttc tttatcacag
    34321 atccaggatt tgcctagaat cgtggaaatt tacaatcaag cgattccaac tcgcttatct
    34381 acagctgatt tagaaccagt tacaatagaa tcgaaaaagg catggtttga tgcccatcaa
    34441 tcaaaagaac gtccgatgtg ggtcatgaag atcgatcaaa atatcgcagg atgggtgagt
    34501 cttagcgatt tttacgggag accagcttat gcaaaaactg cagagataag tatttatatt
    34561 gattcaaatt accgaaaaca tggattagga caaaaagcat tatcatttgc tgaatctcaa
    34621 cttttagatt gtaagatcga tacactttta gcttttgtgt ttgctaccaa tcaagcgagc
    34681 atcaacctat tccgcaaaaa cggatttgag cagtgggggc atttacctaa agttgcaaac
    34741 atggatgatc agttgactga tttaactatt ttaggaaaat cttacgaata ataataatga
    34801 ccgctttcaa gtgtttttac acttggaagc ggtcattatc atagaatgtt tcaattgaaa
    34861 agagtaaagt gaggatgaat ttttttaatt atcatattaa ttaatcaaat aacagtaaaa
    34921 aaaaatcatt tttatctcat atgccattca tttatcattt atcctagtat caatgagaaa
    34981 tagcagaaat ttctaaagaa atgttagccc tttattttag gctgagtatt atcgaacgct
    35041 gattttgaac taactgacga taatataaca ataaagtgat aataaagtaa ttataaagta
    35101 gcaaaaaatt tgtttcttga gaattctgca aaaataagga ttaaaggaag aatgtgtatg
    35161 tcaaaacagt cttttttagt tattgggatt attgctttgc taggtatttt atttttcgaa
    35221 gttacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat
    35281 tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca
    35341 gctaacgtca aagaagccat tcaagaatca gcggaa
//
LOCUS       DACCSK010000015        34522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCSK010000015 DACCSK010000000
VERSION     DACCSK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.015"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(133..306)
                     /locus_tag="IVP39_000660"
     CDS             complement(133..306)
                     /locus_tag="IVP39_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7585757.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IVP39_000661"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IVP39_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IVP39_000662"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IVP39_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7585758.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IVP39_000663"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IVP39_000663"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7585759.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IVP39_000664"
     CDS             5704..6489
                     /locus_tag="IVP39_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7585760.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IVP39_000665"
     CDS             6502..7353
                     /locus_tag="IVP39_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7585761.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IVP39_000666"
     CDS             complement(7477..7929)
                     /locus_tag="IVP39_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7585762.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IVP39_000667"
     CDS             complement(8394..8762)
                     /locus_tag="IVP39_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7585763.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IVP39_000668"
     CDS             8912..9394
                     /locus_tag="IVP39_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7585764.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IVP39_000669"
     CDS             9431..10441
                     /locus_tag="IVP39_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7585765.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IVP39_000670"
     CDS             complement(10556..11269)
                     /locus_tag="IVP39_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585766.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IVP39_000671"
     CDS             complement(11459..12049)
                     /locus_tag="IVP39_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7585767.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IVP39_000672"
     CDS             12533..12760
                     /locus_tag="IVP39_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7585768.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IVP39_000673"
     CDS             12776..13942
                     /locus_tag="IVP39_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7585769.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IVP39_000674"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IVP39_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7585770.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IVP39_000675"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IVP39_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7585771.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IVP39_000676"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IVP39_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7585772.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVP39_000677"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVP39_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7585773.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IVP39_000678"
     CDS             complement(19155..19820)
                     /locus_tag="IVP39_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP7585774.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IVP39_000679"
     CDS             19796..19969
                     /locus_tag="IVP39_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7585775.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IVP39_000680"
     CDS             complement(20014..20751)
                     /locus_tag="IVP39_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7585776.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IVP39_000681"
     CDS             complement(20753..22255)
                     /locus_tag="IVP39_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7585777.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IVP39_000682"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IVP39_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7585778.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IVP39_000683"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IVP39_000683"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7585779.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IVP39_000684"
     CDS             27597..28862
                     /locus_tag="IVP39_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7585780.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IVP39_000685"
     CDS             28866..29597
                     /locus_tag="IVP39_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7585781.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="IVP39_000686"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="IVP39_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7585782.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="IVP39_000687"
     CDS             30613..31611
                     /locus_tag="IVP39_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7585783.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="IVP39_000688"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="IVP39_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7585784.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="IVP39_000689"
     CDS             33123..33773
                     /locus_tag="IVP39_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585785.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34522
                     /locus_tag="IVP39_000690"
     CDS             33960..>34522
                     /locus_tag="IVP39_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP7585786.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11454 a         5832 c         7324 g         9912 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttatactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgtcat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata ga
//
LOCUS       DACCSK010000016        33713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCSK010000016 DACCSK010000000
VERSION     DACCSK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.016"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            149..1018
                     /locus_tag="IVP39_000691"
     CDS             149..1018
                     /locus_tag="IVP39_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7585787.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1227..2252
                     /locus_tag="IVP39_000692"
     CDS             1227..2252
                     /locus_tag="IVP39_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7585788.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2291..3241
                     /gene="manA"
                     /locus_tag="IVP39_000693"
     CDS             2291..3241
                     /gene="manA"
                     /locus_tag="IVP39_000693"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7585789.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3335..5038
                     /locus_tag="IVP39_000694"
     CDS             3335..5038
                     /locus_tag="IVP39_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7585790.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5040..7073
                     /locus_tag="IVP39_000695"
     CDS             5040..7073
                     /locus_tag="IVP39_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7585791.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7084..7524
                     /locus_tag="IVP39_000696"
     CDS             7084..7524
                     /locus_tag="IVP39_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7585792.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7724..8851)
                     /locus_tag="IVP39_000697"
     CDS             complement(7724..8851)
                     /locus_tag="IVP39_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585793.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8854..9531)
                     /locus_tag="IVP39_000698"
     CDS             complement(8854..9531)
                     /locus_tag="IVP39_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7585794.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9795..11561
                     /locus_tag="IVP39_000699"
     CDS             9795..11561
                     /locus_tag="IVP39_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7585795.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11576..12013
                     /locus_tag="IVP39_000700"
     CDS             11576..12013
                     /locus_tag="IVP39_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7585796.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12026..13177
                     /locus_tag="IVP39_000701"
     CDS             12026..13177
                     /locus_tag="IVP39_000701"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7585797.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13239..13937)
                     /locus_tag="IVP39_000702"
     CDS             complement(13239..13937)
                     /locus_tag="IVP39_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7585798.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13930..15282)
                     /locus_tag="IVP39_000703"
     CDS             complement(13930..15282)
                     /locus_tag="IVP39_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7585799.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15505..16905)
                     /locus_tag="IVP39_000704"
     CDS             complement(15505..16905)
                     /locus_tag="IVP39_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7585800.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17066..17803
                     /locus_tag="IVP39_000705"
     CDS             17066..17803
                     /locus_tag="IVP39_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7585801.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17928..18407
                     /locus_tag="IVP39_000706"
     CDS             17928..18407
                     /locus_tag="IVP39_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7585802.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18404..19171
                     /locus_tag="IVP39_000707"
     CDS             18404..19171
                     /locus_tag="IVP39_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585803.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19401..19970
                     /gene="lepB"
                     /locus_tag="IVP39_000708"
     CDS             19401..19970
                     /gene="lepB"
                     /locus_tag="IVP39_000708"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7585804.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20074..20676)
                     /locus_tag="IVP39_000709"
     CDS             complement(20074..20676)
                     /locus_tag="IVP39_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7585805.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20782..21251
                     /locus_tag="IVP39_000710"
                     /pseudo
     CDS             20782..21251
                     /locus_tag="IVP39_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21357..21941
                     /locus_tag="IVP39_000711"
     CDS             21357..21941
                     /locus_tag="IVP39_000711"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7585806.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21944..23017
                     /gene="prfA"
                     /locus_tag="IVP39_000712"
     CDS             21944..23017
                     /gene="prfA"
                     /locus_tag="IVP39_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7585807.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23010..23849
                     /gene="prmC"
                     /locus_tag="IVP39_000713"
     CDS             23010..23849
                     /gene="prmC"
                     /locus_tag="IVP39_000713"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7585808.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23842..24021
                     /locus_tag="IVP39_000714"
     CDS             23842..24021
                     /locus_tag="IVP39_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585809.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24043..25068
                     /locus_tag="IVP39_000715"
     CDS             24043..25068
                     /locus_tag="IVP39_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7585810.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25243..26487
                     /locus_tag="IVP39_000716"
     CDS             25243..26487
                     /locus_tag="IVP39_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7585811.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26640..27587
                     /locus_tag="IVP39_000717"
     CDS             26640..27587
                     /locus_tag="IVP39_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7585812.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27721..28350
                     /gene="upp"
                     /locus_tag="IVP39_000718"
     CDS             27721..28350
                     /gene="upp"
                     /locus_tag="IVP39_000718"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7585813.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28496..28732)
                     /locus_tag="IVP39_000719"
                     /pseudo
     CDS             complement(28496..28732)
                     /locus_tag="IVP39_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28853..28925
                     /locus_tag="IVP39_000720"
     tRNA            28853..28925
                     /locus_tag="IVP39_000720"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28886..28888,aa:Thr,seq:cgt)
     gene            complement(29044..29682)
                     /locus_tag="IVP39_000721"
     CDS             complement(29044..29682)
                     /locus_tag="IVP39_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7585814.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29805..30437)
                     /locus_tag="IVP39_000722"
     CDS             complement(29805..30437)
                     /locus_tag="IVP39_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7585815.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30788..31087
                     /locus_tag="IVP39_000723"
     CDS             30788..31087
                     /locus_tag="IVP39_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7585816.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31582..33713
                     /gene="acm"
                     /locus_tag="IVP39_000724"
                     /pseudo
     CDS             31582..33713
                     /gene="acm"
                     /locus_tag="IVP39_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
BASE COUNT        11640 a         5812 c         6761 g         9500 t
ORIGIN      
        1 tttttataag agtttttact cattaattta caaaaaacaa gcaaaatgcg aattttctca
       61 catttttttt aacaaaatca aagttattgc ttgaaagaag aagaatccat cgttaaaata
      121 aagcttgacg tagaggggag caagaaatat gaaagatttt caattggggg ctgccaccta
      181 tttaaaaagt gtagccattc gggtgaaaga ccgagataaa atgattgatt tttacaagaa
      241 cgttgttggc tttgatttga aaagagaaga aaacgaatta gccattttag gaacagaaga
      301 agctggaagt gaaatgttgc tgctggaaga aagtcctaga gcgaatgatc acatgggggt
      361 gatcaaaaag ctgaaccgtt tttcattgat tatccctagc acagaagaaa tggcagatat
      421 cctttgtcgt atccgtaaaa tgaattatcc aatcaaagaa gctttagaag atcatggacg
      481 tatggggatt ttgctcgtag acccagaaga gaatgaattg gaaatttacc atgaagagaa
      541 taagaacgac caaagagaaa ttccagaaac aatggatcaa gaagcattat taagaaaatc
      601 tgaagaaaaa ttcagtaaac tatcaacagg ttcttatttt gaaaaaatcc atttaaatgt
      661 tactaactta acaaaagaac gccaatttct taatcatgta cttggtttga aggtacataa
      721 tgaagaagag ggcatccatg tattgaatga aggagatttc catgttggtt tgacagaagc
      781 tagcggaggc gcaattgatt tgcctacaga tgaagtgatt ggacttgatt ttctgaaatt
      841 catgatcgat actgaaagca tggaaggact gattgctcac ttgcagcaac tatcagttga
      901 tttcttctta gacaaaaagc acacagtact cacaatctat gatgctatag gaatcgaatg
      961 gtggttcgtc agtgagccaa aagaaaagaa taaaactgac attcgatgcg acgcttaact
     1021 ttttctttct taacgattgt cgcatgtctc gtcattttat gaacggtcat actatcgctt
     1081 attctagaga tagaaagaga aaatagtata ccgggggaaa ctgcgcggtt tttcgcgcag
     1141 tttttttgtg taaaaaaggt tgattcctca tagattaagc tttaagcttg gactgtaaga
     1201 atctgataga aaagcaggag aaaaagatgt atccacacac gaaacagctt attttgaaaa
     1261 agatggaaga aggattttat ccaggtgtag cagtcgcctt cataaaagaa aatcaagtag
     1321 aaacatttac agccggcttt gcccaagtcg ttcctgaaaa gcagctgatg acagaagatc
     1381 tgttgtttga tgcagcctca ctgacgaaag tcgttgcaac cacttcatta gttttacgtc
     1441 tattagaaga tggaaagata gaactcgatc aaccgcttca taaatatctt cctgaatttg
     1501 aaaatcagac aatcactttg cgtcatttac tgacacatac ttctgatatc aaaacatgga
     1561 taccaaacag agataagcta tctcaggaag aattgatcgc cgcttatttg aagctagaac
     1621 caggtgagca actaggaaaa gaagtaaaat atacagatgc cggtacgatt cttcttgggt
     1681 tgatgctaga ggaaatttac caagaaactg cagtggaaat ctttcaagaa caagtattgg
     1741 aaccattagg aatgatgaac agcttatttc ttccttttcc ttccaaaaaa atcgttccca
     1801 cagaacaact gccttcgggt gaggttttgc gtggacaaac tcatgatccg aaagcaagag
     1861 ttttggctga acatgcaggt aacgcgggtc tctttattga tttgaaagac agcattactt
     1921 ttgtccaaat gtatttgaac aagggaaaaa tccgtaatgg tcgtttttta gaagaagaga
     1981 cgattgattc tttactgcaa gatcaaacac cgaaaaaaaa tggctatcgt tctttaggat
     2041 gggacttaaa aaaggatgta gaagacggtc atgcgattct ttttcatact ggttatacag
     2101 gtacgttttt cttgattgat ataaaaacac aagaagcgtt gatatttttg tcaaatcgcg
     2161 tacatcctgt cgatcatcgt gcggagtatc tcatatgtcg agatgaaatt gttgaaaact
     2221 ttttgaaaga gaaagcggta ttaccgaaat aatgctataa tagctatgaa aaatgagagg
     2281 agtttgaatt atgcaggaac ctatgttttt aaaaccagtt tttcaagaaa aaatttgggg
     2341 aggaagtcgt ttacgttcag tctttggttt tgatatccct aacgataaaa tcggagaaga
     2401 ttgggcgatc agtgcgcatc ctcatggtgt cagtgtagta gaaaatggcg aattcaaagg
     2461 gaaaagatta gatgaactat ggtcagaaca caaggaattg tttggccatc cttcagaacc
     2521 agtgtttcca ttattgatca agatcttaga cgcagaagat gatctatctg tacaagtaca
     2581 tccagatgat gcctatggga tgaaacatga aggtgaatta ggaaaaactg aatgctggta
     2641 tatcatcgat gctgaacctg gtgcagaaat catctatggt catcatgcaa agacaagaga
     2701 agaactagct gagatgatca aagatgggcg ctgggatgac cttttgaaga aagtaccagt
     2761 taaaaaaggt gatttctttt atgtaccaag cggaacgatc catgctattg gtaaaggaat
     2821 catgatttta gaaacacagc aaagttctga cacgacttac cgcgtgtatg actacgacag
     2881 aaaagatgcg caaggacaaa caagagagct gcatatccaa caatcgatcg atgtaacaac
     2941 tgtccctgcg aaaacaccag aattacaaat caaagaagtc cgaaaaggga attcatcgat
     3001 cgtcacttat ttagaaactg agtttttcaa tgtatatgag tgggatatta aaggaatcac
     3061 atctttcaaa aaacaagccc cttatacatt gatgacggta atcgaaggag caggagacct
     3121 tgtcgttgac ggaaaaacat atccattaga aaaaggaaca agctgtatta ttccaaatgg
     3181 cgtttctgag tgggcggtcc aaggtgaact agcaatcatt gcgtctgaac caggcaaata
     3241 gttcccagat agctgaaagc agcagacggt ttttcgtttg ctgctttttc tacataaaat
     3301 ccgtccatca ataaatagaa gtctggtgaa aaagatggaa tacaaatgga aagcaaacaa
     3361 cagcagtcgc gtgtctatga aacaactgag aaaagctgga catcacttaa atgaaatgat
     3421 catgccaaat ctaagtctgt ttatcgcttg gggaatcttg tcattagcaa caaaaggtgt
     3481 gtctggcgat ttgcacacca cattagaaga ggtatgccat tggatgatcc agctgctttt
     3541 acccatcttg atcagttatt caggcgggaa atcggtaggg ggacaaaaag gggcagtcgc
     3601 cggtgcaatt gcttctttag ggttgatcgc tcgttccaat gctccacaaa tcgtaggagc
     3661 gatgattatc gggccgcttg ccggcatcgt atatgaacaa tttgtttcta gattccatgt
     3721 gaaattcaag gctggatatg aaatgcttgt cagcaaccta ctagtaggac tgactggtag
     3781 cgttatttgt ataattggaa tcgtttttat tgaaccagtt ttaaatagtg ttcacttgtt
     3841 gctcgcctcc gttgtgtctt ggctagtcac aatgaatctg ttaccacttg tcagtctgat
     3901 cttagaacct ctaaaagtac tatttttcaa taatgcaatc aatcatggtg tattgacacc
     3961 tttaggaatc gaattctcac aaacagtcgg gtactcttcc ttatttttga tggaggcaaa
     4021 tcctggacct ggtttaggga tcttactttc tatcctttgt tttgcagaaa aacaggaaaa
     4081 agtaaatgcg agtggagcat tgatgatcca tgcgattggc gggattcacg aaatctattt
     4141 tccatttgtc ttgctgcgac cgtatctatt tttagctgta atggctggtg gggcaagcgg
     4201 cacgttgata tttcaatggt tagatacggg attagctgca cctgtatcgc ctgggtcact
     4261 actcgtgatt tttgccaatg cgccttatcc acaactagct ggggtaagta tagggatagc
     4321 aatcagtaca tttgtgacct tcatttgtgc agcgatacta ttgaaattac caaataaaga
     4381 aaaagagaac aaaacggtca aagaaagggg agaaatcttg gaaccatcta tcattcattc
     4441 cgtcattttt gcttgcgact caggtatggg atcaagtgct atgggagccg ccctgctgaa
     4501 aaagaaatta gcagcagaaa aaatcgatct ttctgtcgga tacagctcta tctatgaatt
     4561 gaataaccaa gtaaatcagc tggttatcgt gcaacatgaa ctagcgggtc ttgcaagaga
     4621 aaaagtacct caaagtcaaa tctatgtact ggatcacttt ttggaaacag atatcttgct
     4681 gtcacgaata aaagaagcga tccaatttcc aaagcgggaa gaaaaaaaag agatagcaga
     4741 tatcacagaa caaaaaacgt cgcctgctaa tattgtattt ttgtataaaa acaacagacg
     4801 cggctcacaa actatgggca tgacgatttt aagtcaatta gcaaaaaaag cgaaaaaaca
     4861 gtttgttatc tcaaaagaat caatagaaaa aatcaaaatc caagaagaaa cgatttatat
     4921 cgcacaaaga gaactagctg taactgaaca gctagaagaa aagatccctc ggctagttct
     4981 agttgaacat tgggtagcta cgaatgaata cgaaaaactc gtgaagggag aaatataaga
     5041 tgtatttatc gcccagagaa aaacaattat tagcagaatt tctcagtagc ccgtcacctg
     5101 tatctatcca aaaaatgatg aatttattga aggtcagtaa acggactatt taccgcgaat
     5161 tagataatct tgaagtctca ttgaaatcgg taaatgcttc tttgaaaaaa gcagctagag
     5221 gctcatttgt cattgaggca tcagaagaag tattagagca attaaaagaa aatacagatg
     5281 ggaaaagtaa tgacttatcc actgctaaac gacaacacgc gattttagcc gagttgttac
     5341 tgacgacaga gccgttgagt cttgctcatt tcatggaatc atatcgcatc agtaatacca
     5401 ctttttatgc agatatcaaa cagttggaag aaagtattcg ccaactgcct ttggaaatca
     5461 ttcggaacca gggatacgag atcgaaggac cggaaaaata ccgccggctt ctgacagcga
     5521 atgtattaga attagaaatc aatgaatatg aactttttca tagtatttct ttcgatgcat
     5581 cactgaatta tttttttcga ttcgttgatc cgcaacatct atctcttgca cgcaaagtaa
     5641 tcggggaaga attgatccaa aaaaagacca atctaagtga tagaaagctt gaacatttgg
     5701 ttttgatgct gacattgacg atggagcgtg tgacgaaaaa tcatttactg acaaatgaga
     5761 catataatag tctggcaaac aaagaattac tacagacagc taaacgattg ttttcacgga
     5821 tcgcttctga gacaaaacaa ctttatccag taaatgagat cgtctttttt gcaggattat
     5881 taaatgactt cacgaactca tttgatcaga atttctttga agagacgttc gatacacagc
     5941 ttgcttattt agtaaaacaa ctgattcaag aagtaagtga agagacgaat gtccattttt
     6001 atgaagatga aactttgtac aaaatgctgt tgactcattt atctggtgtg ttttcaagag
     6061 ccgttttaca agaagagaca ctgaccaatc cgattttaga aaaaatcatg gttcaatacg
     6121 aggaagttgc gaatgcaatc cgtaaagcag tagagcggat ttttcaagaa aaaaaattat
     6181 ccgaagaaga gatcgcttat atggtccttc attttgcgaa ttcattggaa cgaagtccga
     6241 aagaaagcaa tatcagtatt gcaggatttt cgccaagtgg attagcttct actagcatgt
     6301 tggaactacg attgaaaagg tattttccct tcatccaaca aattcatttc tatcggattg
     6361 ctgatttaaa aaaaatcgat ttacaagaag aatatgatgt tgttatttct acatctattt
     6421 taccaggata cggtggaaaa tatattttag tctccccgct actgctagaa gatgaagtca
     6481 agcagctaaa agaagctttt tcgcatattg agaagaaaaa aaggcaaaaa attacgtata
     6541 acagcaataa cgagtggatg atagaagata cttatgagga aacaatgcat ttcatggatc
     6601 aaatcaatca attactcggt cgttttttta tccaagaact ggataatcct gagacgattt
     6661 ccgaaacgat tcgcagctta tttccttatt ttcctgaagg actagtaacg gatgaagagg
     6721 tggttcatcg ccggctgctt aagcgatatg aacaagcacc tatcggtatt cctcatacaa
     6781 atatggggtt attccataca tccagctcat tgatttctaa accgatcttt tgtattttca
     6841 atttgaaaca tccgttgacc attgaaggga tggatagaca gccagtcgat ttgtcccgta
     6901 tgttagttat gctggcgcct tcgccaatcc aagaaaaaga cgggattctt ttaggaaaga
     6961 tcagtggagc aatcatcatg aatgacttga acaccgagat cttccgctca ggaaataaag
     7021 aaatcgttta tcagttgtta gcgaaaatcc tgattgaaga aataaagaaa tgaaggtgaa
     7081 aagatgatac agctaccagc agaaaaaata ttgctcgatc aatcctatca aaattggaaa
     7141 gaagcgttga ccgagatcgg ccagtggatg acccaacaag ggggaatcaa accggcatat
     7201 atccaatcaa tgatcgaaag acaagaaaaa gcgagtgtct atatcgggaa tttcgtagcc
     7261 ttgccacatg ggactgacaa agatgaagaa gtagtagaag aaggaatcta tttagtccaa
     7321 gtgcctgatg gtgtggactt tggaacaaaa gaggaaccaa aaatcgcgac actcttattt
     7381 gtagtggctt taaaaaaaga acatcagtta gcggtgctcc aagatttagc ctttttctgt
     7441 tcagacatcg accaagtaat ggcgctcagt gatgcggaaa ccattgaaga agtccaaatg
     7501 atttttgaac atggacatat ataaccaatg ttcacagtaa aaaaagctgc cttgcatcaa
     7561 gtaataactt caaccaagat aaaaataaga ggagctattt ttgttgggag gttcttatta
     7621 ctgcggatgc aagcttttga aaactattta ttctagaaaa ccgtttattt gaaatagggg
     7681 acggtgacaa ggtttttgtc accgccccta ttatttttgt gttttaatag ctctttttta
     7741 tatagatagt gcccatacct atgaataata aaacgatact ccatatacca agtgtcaaga
     7801 taccattcat gaaagtaacc tgttctgaag ctagtggcga gaaaatatct cctcctatga
     7861 tcacattttg aaaatcaaca taagaagttg gcaagtaatg actgattgat tctatccatc
     7921 caatcgaaga aaacaagcga tactctgcta agaaaagaca aagaagtgta cctaaaagat
     7981 tgactagaga atgttcaaat aaagcagata gaagaaaact gattgtactt aaaaagagca
     8041 cccacaacaa caggaacaga aagctctgga aaagatactc atacatcggc aggattcgta
     8101 tctcctgacc aatgatcgtt cctacaggat aacggaagtc tcccaaacca tttttgatta
     8161 ttactagtat agagacgatc aaaaatggca tcacgagact tagcatagct gcaccataat
     8221 aaatggtaaa acggatgcta tgtttcttta ccatactatc cggtaataga ttgactagag
     8281 agattacctg ctttcttttt tcataagaga tgatggaaga aagaaacaag gcggttatac
     8341 ataaaatcaa aaaactagaa atcattcctg aaaaaatatt ttcgtagtaa tttgctaaag
     8401 agagatgcgc tggcaagtca aaaatagaat atctttggat ctgatgttct ttcatataac
     8461 ggagcaactc gacacgtttt tgttgttcaa tgattggaat tccttgtctt tgaccagaaa
     8521 tcagtcgatc cagtatatct ttttcataat gataagttgc ttcgaccatt tgctggacat
     8581 tccctgtgtt ttttgcatgt aagattgctt cgatcagttt gttgcttttc tctaaacttt
     8641 ccacaaattc tttctcattc tcatcttctt tcaacgtttc aatcaaacgt tgcatctctt
     8701 catagttatg attcagttca tcgaattctg tataagcaag tttatgatca aaaaaaagtg
     8761 ggaatgcaaa gagcacgacc aacaatagag gaaataaaaa gtttactttg ttttttagta
     8821 agagattgcg ttcaaatgta agtaaagaca tagttacacc tcatataatt tacgataaat
     8881 agctaaaatc tcttccatat ttgaggattc tgtgacgatt tctccatttt ttagaaaaca
     8941 tgctttagaa caaatcgatt ggatattcgc gatagcgtgg gaagaaagaa gaatcgtctt
     9001 cccctctttc tgccattgtc tgatgaggct attcaagata tcaatacttc ctgggtctaa
     9061 tccgttcaac ggttcatcga ataacatgac agatgaatca ctgatcaagt aagcagcaag
     9121 caagacatgt tgtttcattc ctagagacat ttttccaatc ggcaaagtaa ggtactcagt
     9181 catcttcaat cgttggaaaa cagtttggca ttctactttg ctctgccaat agtatttgat
     9241 caaagtcaaa taatcaacag ccgatagata gtcggataac tgattcgttg attctagaaa
     9301 aaagaaagac cggcgataat ctgtataagc agtaggagag atgttgtgaa gggacaattg
     9361 atcataagtt gttggcagca gttgggcaat gccattcagc aaagttgttt tgccaaatcc
     9421 gttcggggca atcaaagcag ctacttctcc tgattctact aaaagattca attgtcgaat
     9481 cacacgtttt ttaccacgtt tcatgttcat gtttttgatc agtaatgtca tagataggcc
     9541 tccaatataa aatttaataa cataatagaa tggaaaacat ctaaattaaa tgctaacaaa
     9601 agttattgtt tgcatattat aaaaaaatta aaataacagt tttatttaaa attgaagttt
     9661 tctgataccg aaatcttttt attttgctga catactcaaa cctttggcac aaagtttcgt
     9721 gccaaagtac gactatttct ctttttagta agcggataca attagagtat caaaaggaga
     9781 gagggagaga caaaatggaa cggacgattc ccaaaaacaa catttcgcta aaagcgaggg
     9841 tacaaaaatt aggcagtact ttatcaagca tggtcatgcc aaatattgga gcactgattg
     9901 cctggggtgt attaacggct ctatttatcc cagatggtta tttaccaaat gaggctttcg
     9961 ctaccatggt aagcccaatg ctaacttatc tgattccttt attgatcggt tatactggcg
    10021 gaaaagtaat tgctggtgac cgcggatcag ttgttggggc aattgcaacg atgggtgtga
    10081 ttgtcggaac agatattcca atgatgctag gcgcgatgat catgggacct cttggtggat
    10141 atgccatcaa gaaatttgat cagctattcc aaaaacggat caaatccggt tttgaaatgc
    10201 tagtcaataa tttttcagca ggtcttatcg gttttggatt agcgttatta ggtttttctg
    10261 cgattggtcc agttgtcgat gcattgactc aagcaatggc aaaaggtgta gagatcattt
    10321 tatctgccca tttgattcca ttgacaagta tttttattga accagcaaaa atcttatttt
    10381 tgaataatgc aatcaatcat gggattttga caccattggg aactgaacaa gtagcagagg
    10441 ctggaaaatc tattctattc ttgcttgaag caaatcctgg tccaggactt ggcgtattac
    10501 ttgcttttac tttgtttggc aaaggagcag caaaatcttc agcaccagga gccatgatca
    10561 ttcacttttt aggcgggatc catgaaatct attttcctta cgttatgatg aaaccgttat
    10621 tattcttatc agttataggc ggaggaatgt caggaagttt cattttccaa ttgatggatg
    10681 ctggactaag agcgccagct tctccagggt cgatcattgc gattctagcg atgacaccgt
    10741 tcaattctta ccttcctgtt attttgggcg tgattgttgc cgcagctgtt tcgtttggta
    10801 tttctgcagt aattttgaaa gcggatgcga aagatacaaa tgaagacttc gaaaaatctg
    10861 ttcaagagac aaaggcagct aagcaagctt ccaaaggtgc ccaaggtatt cctcaaggag
    10921 ctacattctc tggcgtacaa aaaatcattt ttgcttgtga tgcaggaatg ggatcaagtg
    10981 cgatgggcgc ttcgatcttg agaaaaaaag tacaggaagc agacttgcct caaacggtga
    11041 ccaatcaagc aatcagccaa ctgacagatg actcgaatac attgatcgtt actcaggtcg
    11101 aactacaaga aagagcaaaa caaaaagcac cgagcgcgcg ttttgtctca gtagaaaact
    11161 ttttgaattc acctcgatat gatgaaatca ttcaagaatt aagcagcaag cagaaaacat
    11221 cagtttctga aattgagaat gagccagcag aagagtttct ttcagcaaac atcaaacaaa
    11281 tcaaagagat tctacttgtc cacgatgatc gttccggatc agcaacaatg gggatgaccg
    11341 tgctcaaaga tatattagac aagaaccatt taactatccc gttaagaaaa gtcaatatcc
    11401 aagatttaaa agatgaacct gattcactga tcgtagccaa agaaagttta gcggaaaaag
    11461 tacagatggc tgcacctcaa tccattcatt tatcagtcaa cagcatggtc aatccgcaaa
    11521 aatacgaatc gattgcacaa tatttgaaac agacagctta agggaggaaa acagcatgca
    11581 aagtttgcca ttcgacatga tcgagctcaa tcgatctttt aaaaccaaag aagaagcgat
    11641 ccgctattgc ggtcagatgc ttgtagaagc agggtgcgtt gatgaggaat atatcgatgc
    11701 catggtagat agagacaaaa tgttatctgt ttatatgggg aattttatcg cgattcctca
    11761 tggaacagat gaagcaaaaa aatatgtgaa aaaatccggc atctgtgtcg ttcaagtacc
    11821 agatggtgta gattttggta ccgaagaaga agaaaagatt gcgactgttt tgttcggtat
    11881 tgcaggagtc ggagatgagc atttgcaact tgtacagcaa atcgcacttt attgcagtga
    11941 tatggacaat gttgtccagc tagcagacgc gctcagcaaa gaagaagtaa cggaaaatct
    12001 agcaattgct taaggagaga gaaaaatgag agcagtacat tttggtgcag gaaatatcgg
    12061 tcgaggattt attggagagg ttttggcagc taatggattt gagattactt tcgtagatgt
    12121 gaatcacacg atcattgatg ctttaaatga aagaaaagaa tacgaaatcg aattagcaga
    12181 tgaatcaaaa gaaaaaatca ctgtaaaaaa tgtcaaagga atcaacaatc aaacaaatcc
    12241 agaaaaagta atccaagaaa tcgctgaagc agatttagtt actaccgcca ttggcccgaa
    12301 tatcttacct tttattgctg agttgattgc aaaaggtatc caagaaagag aaaaggaagg
    12361 aagcacaaca tctttagata tcattgcctg tgaaaatatg attggcggtt cagcatttct
    12421 agaaaaagaa gtgtataaat atcttccgga aacatcattt actgacaaat atatcggctt
    12481 tccaaatgca gcagtcgatc gaatcgtccc attacaacac catgaagatc ctttattcgt
    12541 ccaagtggaa cctttcaaag aatgggtcat tgacgatagc caaagaaaaa ataaaaaaat
    12601 ccaattaaaa ggggttttat atgtagatga tctagaacca tatatcgaac gcaaactatt
    12661 tagtgtcaat actggtcatg cgactgtcgc ttatacaggt gccttgttag gttaccagac
    12721 cattgatgaa gcaatgcaag atgcgttagt tgtggcacaa ttaaaatcag ttttgcagga
    12781 aacaggaagt ttgctgattg ccaaatgggg gtttgatgca gagcagcatc atgcgtatat
    12841 tgaaaaaatc attcatcgct tccaaaacaa aaatatctct gatgcgatca cacgtgtcgc
    12901 tcggacacca ttacgtaagt taggttatca agaaagattt acacgtccag tgagagaact
    12961 acaagaacat aacttgactt gcccacattt gacggcaaca atggggatta tttttaatta
    13021 ctatgatcct gaagatgaac aaagccgtca attgcatgaa atgaagatcc atgaaaactt
    13081 ggaacaattg attcaagaag tcacaggaat caatgatccg aaaacaatcg ggaatatcaa
    13141 gcaaaacgtg aaccgttatg ccaagcaagt cgcttaaaat aggatacgaa aaaggggaat
    13201 gtgacgaaat ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga
    13261 tagggcagat ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct
    13321 gccaggttct catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg
    13381 taaatgacac cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc
    13441 aaaggtcgtt caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa
    13501 gtttcattga attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga
    13561 cttaacgtgt agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg
    13621 tagtagtgac ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg
    13681 atatagttga tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca
    13741 aagtcttgac caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt
    13801 atactaacga tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc
    13861 aaaaggtttc cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg
    13921 agttcataat tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt
    13981 acgaagctgc aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc
    14041 tttgattttt tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat
    14101 cccggcaact tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc
    14161 tgggatatcc atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat
    14221 tccgtctgcg taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc
    14281 aatcatatcg attacttgat tgatacctac aggatttttg acaagtacta atgtacattt
    14341 tttcccattg atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc
    14401 ttgtttgatt ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc
    14461 attgtagaca ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa
    14521 atctgctgac acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg
    14581 tttgaagtca caatttggac agtaatattt ccctaagttt gcataagtga tcattttata
    14641 atgcaagatg tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg
    14701 ttcacgatct tcttcatggt caaagccata atattttcta ggattgactg tttcgataga
    14761 attgaagatt ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc
    14821 gtctacaatc attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa
    14881 aatatttgta aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc
    14941 ttcatctatt tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa
    15001 agtcgataca attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa
    15061 ttgctggcgc aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt
    15121 gacaacgacg acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag
    15181 cgctagttta cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga
    15241 cgtttttcca actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac
    15301 gatttgctga cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc
    15361 ttttcttatt cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat
    15421 gatttaacaa aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta
    15481 ttccctctta cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata
    15541 gcctagatct cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg
    15601 cccaataaac atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat
    15661 ccctaaagat aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag
    15721 cgtttgttct tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag
    15781 cataaagaca acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt
    15841 gatgttatct tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt
    15901 tgttcgaatc ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa
    15961 aatgatcagt gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg
    16021 agtcgtaatc gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa
    16081 taaatggtcc ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa
    16141 agctaacaca gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg
    16201 tttgtataat agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa
    16261 aatcaaaaac atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc
    16321 cccagtgacg tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa
    16381 aacggcatca cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt
    16441 cccaaaaagt aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat
    16501 accagacaaa cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat
    16561 ccgtcgttta gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac
    16621 ttgaaataat atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa
    16681 agtcgatagt ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga
    16741 aacatggcta tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt
    16801 catcaatata taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt
    16861 ctgtcctcgc cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg
    16921 acaatagtcg tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa
    16981 cagataacaa tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga
    17041 aaaaagaagg aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa
    17101 aaatcaaaaa ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga
    17161 agaagtaaag cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt
    17221 ggaattcatg tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc
    17281 taaaagtgag tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga
    17341 gaaaactgac catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa
    17401 aatggtttta acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg
    17461 cttcaatatg cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt
    17521 tggaagtgcc ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat
    17581 ggatatccaa aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg
    17641 ctatgaacgc tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg
    17701 ttgcgtgttc gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa
    17761 agcttactta aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa
    17821 acgtaagaaa aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt
    17881 agtaaagtaa ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag
    17941 cacctatttt gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag
    18001 gtattgtgaa tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa
    18061 caacaccaac tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa
    18121 ccactacgac aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata
    18181 caactgacag ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa
    18241 ccaatccaag tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga
    18301 ctgtagatga taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt
    18361 caggaaatac ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga
    18421 ccttatcaac ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa
    18481 ccgtggatat acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg
    18541 actttggcaa aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct
    18601 tgtttatttt actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga
    18661 tgtcactcag ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga
    18721 atcgatagat aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta
    18781 tgacgaagat tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga
    18841 gatcaaaaca aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta
    18901 tccttattac tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag
    18961 ctactcggac atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga
    19021 acctgtcgga ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga
    19081 gccttatcgt ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta
    19141 tagtcgtagc caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg
    19201 cgataaaaac gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt
    19261 ttatataacg agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca
    19321 ttgtagaata aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa
    19381 atacaagtag gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg
    19441 tattagcggc tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc
    19501 attcgatgga ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact
    19561 atgaacgaat ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta
    19621 aacggatcat cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta
    19681 atggcaaaaa gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata
    19741 agttacaagg agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca
    19801 cctttacgag tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata
    19861 accgcttgat ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag
    19921 gaaaagttgt tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata
    19981 aaaagaagct gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt
    20041 cttggagttg ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa
    20101 aagcgaagcg tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc
    20161 agagacaaag cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg
    20221 gactttcgga tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc
    20281 aggagctaaa atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag
    20341 tacaagtgat agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc
    20401 gtggatcgtt cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag
    20461 catagtttct gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact
    20521 aacaagccca gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag
    20581 ataagtagtt agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata
    20641 atggttagcg atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt
    20701 tgaaatttta acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa
    20761 aagggagaat ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga
    20821 aagtgtgtcc gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg
    20881 gaaaaatata tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa
    20941 tatttaggct ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt
    21001 gtatgggaat aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag
    21061 cagaaggtta cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc
    21121 actactctat atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg
    21181 aagtttttcc aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg
    21241 aacaaaagtg agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa
    21301 aaatgctgaa aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg
    21361 cacaactatt ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag
    21421 tcgcacataa ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata
    21481 caagagatgg tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga
    21541 tatttgaaga aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg
    21601 tgctgatcga tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg
    21661 tggacgaatt ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt
    21721 tatttgaagg atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa
    21781 tctgttggtt ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg
    21841 tttatgaagg cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa
    21901 aacattactt tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca
    21961 agcaattgaa gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc
    22021 tgatacaaag cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga
    22081 agtttatcgt cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg
    22141 tgaaaaactg gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa
    22201 agaaaaagaa gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga
    22261 cgataaaaat atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt
    22321 cgctggcgat cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga
    22381 agtgatggaa gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac
    22441 cggagacaac gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt
    22501 tccttcaact gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc
    22561 agaagctgaa gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca
    22621 tgcttcaggt gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca
    22681 cttaccaaca gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga
    22741 aaaagcaatg aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag
    22801 cgagtatgat gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg
    22861 gacgtataat ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa
    22921 attagatcag attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga
    22981 tcaaacatca aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt
    23041 cacgggcttc ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat
    23101 ttttggaacg aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt
    23161 cagaagaaga acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac
    23221 ctcagtattt gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa
    23281 cactgatccc tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg
    23341 gtgtaccgtt agaagtgata gatattggga cagggactgg agcaattgct attagtttga
    23401 aagcagcaag aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag
    23461 tagcgaaaga aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat
    23521 taggaccagt aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc
    23581 ggaatgaatg ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt
    23641 ttgctgagaa cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac
    23701 taaaatcaaa tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa
    23761 aaatatttca gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca
    23821 atgaacgaat gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt
    23881 tttattataa aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa
    23941 gtttatccac tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc
    24001 agacaaaata agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat
    24061 gatgaaacag aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt
    24121 cctaccgaaa cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa
    24181 gtgttttctg taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt
    24241 gaacaagtaa aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac
    24301 ttctggcctg gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt
    24361 gtgacgggag gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa
    24421 gtaatcgaac tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca
    24481 agtccaacga ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt
    24541 gatgatggag ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca
    24601 gccatgccta tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc
    24661 gaatctcctg ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct
    24721 gggatgaaat acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat
    24781 tggtcaactg cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc
    24841 cccgaaattg cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac
    24901 tcagtagaag cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca
    24961 cttggattag atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac
    25021 atgaatcgct tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat
    25081 tgtcgcattc ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa
    25141 aactaagcgt aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg
    25201 ggaaaaataa aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg
    25261 tttgaccctg atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg
    25321 gagctgattg cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc
    25381 ttgacaaata aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt
    25441 gtcgatatcg ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt
    25501 gcgaacgtcc agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta
    25561 gaaccagggg acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga
    25621 tcaccagtca acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca
    25681 acagaagtta ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg
    25741 atcgtagcag gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc
    25801 gcagatgaag taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca
    25861 gcaggacttc atcctaatcc agttccttat gcagatatca caacgactac cactcataaa
    25921 acattgcgtg gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa
    25981 ataaatagtg cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt
    26041 aaagcagtag cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc
    26101 atcgccaatg caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt
    26161 tcaggggcga cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga
    26221 aaagaagcag agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct
    26281 tttgaaacac taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact
    26341 actcgcgggt tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta
    26401 caagcaaaag atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact
    26461 gaaaaatttc cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc
    26521 aatgttgggc ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg
    26581 ctctctagta tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca
    26641 tgactgatcg tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc
    26701 atccgcgtat ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag
    26761 aatcacctgt gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat
    26821 ggggaaaact caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta
    26881 ttttttatat tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga
    26941 tcagagaact tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac
    27001 cagaagcaaa atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata
    27061 aattagccaa gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag
    27121 gtggtaacat ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat
    27181 ccaagcaatt gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg
    27241 aattcggaga aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata
    27301 cgacgaatga agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg
    27361 aactaaatga tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg
    27421 aaggtgaggc ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct
    27481 tccaaggaat catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc
    27541 gtgcagcaat ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc
    27601 gcaggaaaac ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc
    27661 aatcacttga ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa
    27721 atgggcaaat ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg
    27781 gacaaaaact gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg
    27841 gcatatgaag tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact
    27901 gaaacaacac aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca
    27961 ggtattggaa tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc
    28021 ggtctttatc gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa
    28081 gacattgatg cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca
    28141 atcatggcaa tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg
    28201 gtagcagcac ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat
    28261 acagcttctc tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat
    28321 gcgggtgacc gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt
    28381 ttgtcacatc cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt
    28441 tcgtttgcat ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt
    28501 ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag ttcgcatttt
    28561 ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa atcgttccag
    28621 tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa
    28681 tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc ataaaacctg
    28741 cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa ctaattttcc
    28801 atcggactct ttacaatcag ccaaatagag tatataattt cttttggata aggccagctt
    28861 agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc
    28921 tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata catctgtctt
    28981 tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta tttctataaa
    29041 aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca catgaactgc
    29101 ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca atcttgccac
    29161 cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat cataagtact
    29221 gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa tgctgaactt
    29281 actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta tcatgatagg
    29341 caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg ttaaacccca
    29401 tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat tgataaaaaa
    29461 atatttggct atatttttga caggagcgac aggtttcgag aacatttttt tcgtatcttt
    29521 tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa aaatgctaat
    29581 aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag tgcccaatat
    29641 ccctttttta agttctattt tttttcctaa aatgttattc atgataataa ctcctactat
    29701 tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat gtttttttat
    29761 ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac tacttttatt
    29821 tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca attttttaga
    29881 tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat attttttaaa
    29941 tcagtatcaa aaaagctagc attttctaaa tcattagtag aaaacattat tttagtgagg
    30001 gaacagtcta caaaatctgc gcttgataag acagagtgct caaaggtact ttcttttatg
    30061 tttgaaccag ataaatttac atagtccatt ttacaattct cgaataacgt gttcccaata
    30121 taactttcca tcaatttagt acctgttaat ttacaattga caaatttcac gcgatgcaac
    30181 atgctttttg tcaatataca gcctgaaaaa tcacagtttt cgaatataca gtcggtaatc
    30241 tccattcttt gaaaatcatt ggaagcaaaa aatgcatact tgaaaataga tttgtaaata
    30301 attggatggc agattatttg gttttcgatg acctcttttt gtgaaaggat attatacagt
    30361 tcaaaatctt cgttttcata gatatgaaaa aaggatcctt tattcaagct tgcttcatta
    30421 actttaggaa aaatcatatt taattacctc cattttcata aagtcatatt aacaaaagct
    30481 aaaccaactt acattagcag aaaatgagat aggttttcgc ctttttcgat aacgatagtg
    30541 tcgctatctc ttgaaacgtt cttagaattg ccctgagaga cacaaattag gcgtaattct
    30601 aataaaaatc ttagaataaa aatttttact gatttaattt agaatgtttt taaaataaaa
    30661 atcgttgcca taacagtgta ttgaaaggtt ttaacaatcc agtaatgatt taaaaaaatc
    30721 tagatgtagc gtatactttg tttatagaat gtagtgggaa gaaatatatt tttgggagga
    30781 aatcgtgatg tctaaaagtg aagaaataga aaacatggta ctaaaattat atgatctcgt
    30841 tttaaatcca gagatcaaag aaaaggaacg tatacttttg atagacgcaa agacaggctt
    30901 agaaaaaggt cagtattatc ctaaagtcat aaataatttg gaacgtagtt tacgtccgtt
    30961 agcaatcagg ggagaattgt ctaaaccagt atcaccgttt tatatggaga tatcaacaat
    31021 aggaaaattt gaaaaagaat taggtcgagg aatggcttcg gcaccgatta cttttgggca
    31081 tttataaaaa cgagttattc atctcaatga attaaaaagt atagtcaatc tcataatgca
    31141 ggctaggttc atttatttga acagagtctg tattttttta ttctaaataa agaaacagat
    31201 gtttctttat attgtatttt atcctttaat taacacaaaa acagctagta taaactaata
    31261 atgttaatta aaagtgtatt ttatgaaact atcgttattt attaatgttg catttttgta
    31321 agttaaaaat tggaaatata gattgaaaaa atgaaagcaa taagtaaaat aaaatgaata
    31381 tatgcgaaaa aataatttac attaggaaaa accttgtaaa aacagttttt tggcatattt
    31441 attttttata aatatttgta aaataactat caatttttta gttaaaagat tggaaatgag
    31501 tgggggagga attgaagaaa ggatttttga gagatgatat agtaggtttt atttttagtt
    31561 aaaatagggg agatagaaaa aatgaaaaag tgtagaaata ttattttatc aatgttattt
    31621 attattacta atataacatc actgattcct gttcatgtct atgcggatgc aggcagagat
    31681 atcagcagta atgtcacttc gttgaccgtg gacccaacga atattacaga tggaggaaac
    31741 ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg tgattatctt
    31801 tggatcaatt ggccaagtga aggaaatatt cgcggtgaag gattccaaaa agaaatccca
    31861 ttaatgattg aaaataaaaa tgttggaact ttaacggtca gaaaagattc tgcgcaggtt
    31921 gtgtttaatg aaaatattaa aaacctagac tcagtagaag gttgggggca atttgagata
    31981 caggccagaa acgtaaccga taccggtgaa gaaaatacgg gaccattcac cgtgacgagc
    32041 ggtgataaaa cagctactgt aaatgtgaca aagccagctt ctggttcttc ttctagcgtt
    32101 tttttattat aaaacaggag acatgctacc tgaagatact aagcacattc gatggttttt
    32161 gaatattaac aataacggca cttatgttga acaaccagtt aaaatcagtg atgaaatcca
    32221 aagtggacaa agattagatc caagtacgtt tgaaattaat caaatacact taggtgaaca
    32281 gaaggtttat agaggagaag agggaatcca acaattttta caagatttcc ccagcgctac
    32341 atttaatttt tcagtaactg ataattacat tgaaataact ataccaaaaa actttgtcaa
    32401 tcttaggaaa ataatggtga gttataagac gatcatagaa aacccagaac aaataaattt
    32461 tgagaatcat tcggaagctt ggtttaaaga atttaataaa cctgctgttg atggagaaag
    32521 tttcaatcat acagttaaaa atatttcagc ttctggtgga gtcaacggta cagttagagg
    32581 cgaattgaag attttcaaat atatcaatga cacagagatt ggtatcccaa atgtgacttt
    32641 cgaattaaga cgagcagatg aacagcctat tcaaggacag tctagtatcc tgttgacttc
    32701 gaatgaacag ggagaagcta gtatcaaagg cctgcaagta ggagactatg ttgtaaaaga
    32761 aaaagaagca cccaattgga ttgattttga tccactaagt tcaaatgaac tcaaattctc
    32821 cataaacgaa aatgatacag agggagtcag tttacctatt tataacaaga aaaaggttac
    32881 gaacatcaca gcaactaaaa tttggaacgg tggaaccact ccaagaccga gtatttattt
    32941 taaattattc agagcaagta caaacaattg ggaaccagtt cctgatgcag aaacaaaacg
    33001 attagacaat ggaataactt cagtgacttg ggaagatatc caacagtatg atgatagtgg
    33061 aaacgaatat acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc
    33121 aggttatcaa aaaattgaaa acgggctagt cgttacaaat gagaataaag aagtcatttc
    33181 agtctcagga caaaaaacat gggaagacaa tgagaaccaa gacggcaaac gaccgacgac
    33241 gatcacagta aatctattag ccgacggtca accgatccaa cacaaagaag taagtgaaaa
    33301 agatgattgg cgctatcgtt tcacaaatct gccaaaatac agagacgggc aagaaatcat
    33361 ctatactgta acggaagaca acgtgcccga atacagtacg acgattgacg ggtataacat
    33421 caaaaacagt tatacaccgg aaaaaacaaa tatatctata ccaggtagcc atataacacc
    33481 gtggaaaaat tttccagaga agacagagag agagaaaatt agaaatctat tctctcaaaa
    33541 attgcaaata tttgctcata caggagaatc cagcgaacaa agaagcaatt atattagtaa
    33601 gagagctgta cctaaacaga taacaaataa atcaaaaagc attcttccta aaacaagtag
    33661 taaaaaatct agttttgctg taatcattgg tttattgata gtaactatct gtg
//
LOCUS       DACCSK010000017        32553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCSK010000017 DACCSK010000000
VERSION     DACCSK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.017"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(94..390)
                     /locus_tag="IVP39_000725"
     CDS             complement(94..390)
                     /locus_tag="IVP39_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7585817.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="IVP39_000726"
     CDS             746..1102
                     /locus_tag="IVP39_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7585818.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="IVP39_000727"
     CDS             1112..2011
                     /locus_tag="IVP39_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7585819.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGKLLDHYSNIKQVVF
                     GHTHRRFEDQLIHGAIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2528..3259
                     /locus_tag="IVP39_000728"
     CDS             2528..3259
                     /locus_tag="IVP39_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7585820.1"
                     /translation="MMKKEIVLTATTMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRGTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3404..5431
                     /locus_tag="IVP39_000729"
     CDS             3404..5431
                     /locus_tag="IVP39_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7585821.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENENLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5539..6636
                     /locus_tag="IVP39_000730"
     CDS             5539..6636
                     /locus_tag="IVP39_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7585822.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMRQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVIIETKITKKGHSKVLYREEKEALQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6942..8410
                     /locus_tag="IVP39_000731"
                     /pseudo
     CDS             6942..8410
                     /locus_tag="IVP39_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            complement(8503..9237)
                     /locus_tag="IVP39_000732"
     CDS             complement(8503..9237)
                     /locus_tag="IVP39_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7585823.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            9563..9973
                     /locus_tag="IVP39_000733"
     CDS             9563..9973
                     /locus_tag="IVP39_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7585824.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            9966..10667
                     /locus_tag="IVP39_000734"
     CDS             9966..10667
                     /locus_tag="IVP39_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7585825.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            10667..12280
                     /locus_tag="IVP39_000735"
     CDS             10667..12280
                     /locus_tag="IVP39_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7585826.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHTFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            12270..12491
                     /locus_tag="IVP39_000736"
     CDS             12270..12491
                     /locus_tag="IVP39_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7585827.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDECDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            12488..13249
                     /locus_tag="IVP39_000737"
     CDS             12488..13249
                     /locus_tag="IVP39_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7585828.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPEIFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            13629..14138
                     /locus_tag="IVP39_000738"
     CDS             13629..14138
                     /locus_tag="IVP39_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7585829.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKIAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(14209..14748)
                     /locus_tag="IVP39_000739"
     CDS             complement(14209..14748)
                     /locus_tag="IVP39_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7585830.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVAVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(14888..15499)
                     /gene="rpsD"
                     /locus_tag="IVP39_000740"
     CDS             complement(14888..15499)
                     /gene="rpsD"
                     /locus_tag="IVP39_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7585831.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            15815..16612
                     /locus_tag="IVP39_000741"
     CDS             15815..16612
                     /locus_tag="IVP39_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7585832.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAYGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            16640..17275
                     /locus_tag="IVP39_000742"
     CDS             16640..17275
                     /locus_tag="IVP39_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7585833.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLTLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            17295..18068
                     /locus_tag="IVP39_000743"
     CDS             17295..18068
                     /locus_tag="IVP39_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7585834.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHKDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            18476..19273
                     /locus_tag="IVP39_000744"
     CDS             18476..19273
                     /locus_tag="IVP39_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7585835.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            19251..19664
                     /locus_tag="IVP39_000745"
     CDS             19251..19664
                     /locus_tag="IVP39_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585836.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            19648..22293
                     /gene="mutS"
                     /locus_tag="IVP39_000746"
     CDS             19648..22293
                     /gene="mutS"
                     /locus_tag="IVP39_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7585837.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAADVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            22311..24419
                     /gene="mutL"
                     /locus_tag="IVP39_000747"
     CDS             22311..24419
                     /gene="mutL"
                     /locus_tag="IVP39_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7585838.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKFVEEDEKIYEEELALHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            24441..25001
                     /locus_tag="IVP39_000748"
     CDS             24441..25001
                     /locus_tag="IVP39_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7585839.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(25086..27074)
                     /gene="treP"
                     /locus_tag="IVP39_000749"
     CDS             complement(25086..27074)
                     /gene="treP"
                     /locus_tag="IVP39_000749"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7585840.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            27297..28010
                     /gene="treR"
                     /locus_tag="IVP39_000750"
     CDS             27297..28010
                     /gene="treR"
                     /locus_tag="IVP39_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7585841.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFIEFARRRKV"
     gene            28087..28533
                     /gene="msrB"
                     /locus_tag="IVP39_000751"
     CDS             28087..28533
                     /gene="msrB"
                     /locus_tag="IVP39_000751"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7585842.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            28703..29305
                     /gene="ruvA"
                     /locus_tag="IVP39_000752"
     CDS             28703..29305
                     /gene="ruvA"
                     /locus_tag="IVP39_000752"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7585843.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            29318..30319
                     /gene="ruvB"
                     /locus_tag="IVP39_000753"
     CDS             29318..30319
                     /gene="ruvB"
                     /locus_tag="IVP39_000753"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7585844.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            30348..30926
                     /locus_tag="IVP39_000754"
     CDS             30348..30926
                     /locus_tag="IVP39_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7585845.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            30978..31460
                     /locus_tag="IVP39_000755"
     CDS             30978..31460
                     /locus_tag="IVP39_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7585846.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIGENKITNNL
                     "
     gene            31577..31951
                     /locus_tag="IVP39_000756"
     CDS             31577..31951
                     /locus_tag="IVP39_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585847.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT        10809 a         5671 c         6810 g         9263 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaataaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ttggaacaaa ttaaatgcgt ttttaggatc agaatcattc ggaaaattac tagatcatta
     1741 ctcaaatatc aaacaagtag tttttggtca tacgcatcgg cggttcgaag atcagttgat
     1801 ccatggagca atttatagtt gtcgaccatt cggttattat tatgaatggc aactgactcg
     1861 tgattttgtt ttgaaagaac agttgttaga cagctatgat cctatgaaat tacgtgtact
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag tcacattttg
     2281 atagatagga gggggactta cgaattattt taaaggctat gacagctgta tttctagatt
     2341 gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa tcatttataa
     2401 ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa gtatcgtaaa
     2461 acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata aagaaagagg
     2521 aatgtcaatg atgaaaaaag aaatcgtatt aacagcaaca acgatgttat tcagcatggt
     2581 agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata acacagaagg
     2641 aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc cagagaatcc
     2701 tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag gaggggcatt
     2761 atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta ctgcggataa
     2821 aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga catatgtcca
     2881 agtgacagat aggagaggta ccttatctgg gtggaaatta agtgtctctc aaccagaaca
     2941 gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta cgaaagggca
     3001 ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg aattaacgtt
     3061 gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag gtgtggggac
     3121 ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac agctttcggt
     3181 acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt ggacattaga
     3241 agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac gtagtcatat
     3301 tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg tttggatacg
     3361 aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa agaggaggat
     3421 cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag ggtactcttt
     3481 ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac tcacttcgac
     3541 attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt taagtatttc
     3601 gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac aaggactgga
     3661 agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt ctatcgataa
     3721 aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga agttactgca
     3781 gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga agattagttt
     3841 actctcggat tttacattta aatcttattt atacttagga aatcaacgag atagagcggg
     3901 agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc ccagtcaagt
     3961 tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc aaccttacat
     4021 tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt ctgatcgaat
     4081 ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg tcacgcctaa
     4141 accaaataat aataattatt ctggagagca tactggcgtg acggtagctc ctacatttct
     4201 ttctaatggc acatggcgct tgttaataat taaatgggat gcggagcaaa gaagtcttac
     4261 ttatgatttg gaaggtgtcg gtcgctcttc tcggattata cctgatctca ttaggcagtt
     4321 tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt ttcaagaaaa
     4381 tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg taaaagtcaa
     4441 cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa ctatcagaaa
     4501 tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta gtatcgacct
     4561 cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa ttgatgccaa
     4621 agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt ttattgctgg
     4681 agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg aaaaagtact
     4741 cactatgcat tttgaatacc ttgaaaacga gaatttgatt gagcaatcga atgaaataaa
     4801 cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag ataaagacag
     4861 tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga agtataaaga
     4921 aactcctatt gatattgaag ggtatgtctt tgactcggat tcagcgaata cagaaggcat
     4981 cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac tttatctttc
     5041 aactgctcct caatatgtca attttggaaa acataaaata tccagtagac cattaagtga
     5101 attcggacag ttttcaggtg gattgaaaat tatagatgaa cgggcttttg gaagttggaa
     5161 attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc cagaagctct
     5221 ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta cgattttcga
     5281 aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag ctcatcgtgg
     5341 gatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg ggaaactatc
     5401 ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa caaaattatt
     5461 gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac aagatattgt
     5521 tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa acaacaatta
     5581 tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt gtcagctagt
     5641 gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa gagaaaaact
     5701 tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga attgagaaat
     5761 gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac caattcaaat
     5821 gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat ctttgatctg
     5881 gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt ccaaaccgtt
     5941 gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg tgggatcaca
     6001 ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag cttatcgatt
     6061 gagaatgaat atacctatat tattgctttg ttgatgcgac agacactgaa cgaagttgct
     6121 ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa tgtcatctta
     6181 gcaaacgttc aaaatgatca aaaaacttac atcaatcaag taattattga aacaaaaatt
     6241 acaaaaaaag ggcattctaa agttttatat cgagaagaaa aagaagcgtt gcagattgct
     6301 ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac cccaggtgaa
     6361 tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt ttcgagaaaa
     6421 aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga ttttacaata
     6481 gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa agacaataat
     6541 tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg catacttttg
     6601 tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa atagtaaatt
     6661 tttattattg taggatggtt gggctctttt tataataata taaaatatac cattttatta
     6721 ataaaaatgc atactagtaa atgatataat tcttataaca ggtttgatcg cgagaaaggc
     6781 caaataaaac gctctccaaa atgttaaaga aagacaggac gatgtatatt atacaaacaa
     6841 gaatcgctga caacagtgac tcggatccag gaaatcagtt ttatccccag ctagttggtg
     6901 aatgcatgga aagtaatata cgtagaaaga gctgatgact aatgaattat gagttttttt
     6961 taagcaaaga agatcacaga agttattcat tacttaaata tttagaagca agctcaaatt
     7021 tatcagagtc aatatcgaat gttcaagaag agttgtcgct atcgacattt ctcttgaaaa
     7081 aaacgatcga taagctgaaa catgatttac aaaagttcga tctagagaaa aatttgcgag
     7141 taaccgtcag tgaattggat atatgtctgg aaattaatgg aaaatgttct agcaaagctc
     7201 tgttatctcg ttatataacg gattctttat ctatgaaaat ggtattggct ttctttcagg
     7261 aaaaatatat ctcagtagaa aattttgcta aacagcatca cgtgagttat tctgtggctt
     7321 ataaagtatt acaaagacta aaaagaaatc taaaaaaata tcaaattttt ttgaaaagag
     7381 aaagttgaca gggaatccta aaaacattcg gcttttttta tataatcttt ttaactcaac
     7441 agagataagt tttgtggaat tgtatacgct acctataatt ataaaaacag aaaaaatgtt
     7501 aaaagattta aaacagtatt atcctctgtc tgcttatgaa aaaaagaaat tattccactt
     7561 tttagcaatc aacctgttgg ctgaacaagc tgattcttta gatattgcta gcaatcagtc
     7621 gctatttttt tcggatatag gattttacca cttaaaagga gtattaggag aaaaatccgt
     7681 tatttttatc aataagacac tctcttggct ttatcttcat gataaattgg atcaaaaata
     7741 cttgagaaaa aatgaagatg aaacaatcga atggttgaat gaacgattca ttgaaagttt
     7801 tgaaaatcag tttaatctgt tgcgagaaga gacgagccaa ttgatccgaa gagagttaac
     7861 caaaatacat tttaaacttt tgcattacac tattaataaa gctgatgaat ttccacttga
     7921 tgtttctttt tttagagaaa actatccagc attttattgc tatttgatag agtatataaa
     7981 tgatctaacg tcaaaacgca aagagttgct tcaagataaa ttttttcttt ttttcagtta
     8041 tttattatta ttgatcaatc acatacccat tcaactgatt tctgaaccag taaagatcat
     8101 catcgatttt tcttatggtg ctgcctataa tcaatttatc aagaaaaatt taagcttgta
     8161 cattaatctg aacacggaag tgatagaggc aggaaagcct gagttcccgg atgttataat
     8221 cacgaatagg aataatctat atgaagaaag tgcatctcaa gtagtggttt ggctcgatcc
     8281 gccaagagca gtagactggg ggaatcttac aaagattttg ttgactatcc aagaagagaa
     8341 atacaaagac agcaaaattg acaagcaaat agacgatttt cctgaagaaa tcatcgacag
     8401 gatcgaatag tgaacttctt ttagaaagtc cgactgcttt ttctaaaaat aaaaaaactt
     8461 ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca taaataataa
     8521 tttatataag aatactgcta cgacaccagc acagataggt gctacaactg gtacccatgc
     8581 atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta caagacgtgg
     8641 agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg aagcaactaa
     8701 tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt ggaagaatgg
     8761 tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga attcattgac
     8821 gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta acacatggtt
     8881 cacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca attgtccaat
     8941 cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag ggaatagacc
     9001 attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga ttccaccaaa
     9061 aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca tccagtcact
     9121 gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac ctagaacgat
     9181 tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt aatccataaa
     9241 tacctccaaa agttataaaa atgcaaaaaa aaagaaacat caacacgaaa agcatcggtg
     9301 cagtgtttcg tgtcggaagg atttctgcag tcttaacaaa tgtgaataaa aaaatgaatt
     9361 ccattactta taagaaacca gaccaacagt aaaactccaa ctctttttaa aagttctcca
     9421 caaatcacca agcttaacta taaatatacc aaaaaaaaaa cgtttttttt ctcaaaaatt
     9481 gtatcttaga aacagcatgt tttgaaactt tcagaaaagg tttgttagta aaatgaaagc
     9541 tatacgtaag gagtgttgtt tgatgaaatt ttttcaagag tttttcatta ttattttctt
     9601 ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg gaagtatctt
     9661 aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg aaaaaatcgg
     9721 agcaactggt gattttttat tgaataatct agctatttta tttgttcctg ctggtgtagg
     9781 tttattggaa tattttgatg atattgcttc gatatggcct gttttactgg gagcagtcgt
     9841 cgtgtgttcc atcgtcacga tggttgcagc gggaaaaacg gcagaaggtg tagaagcttt
     9901 gctgggctat gtccgtaaga aaaaagcagg aagggtatct gtaaacgaaa tggaggaaaa
     9961 tcaaattgac tgagtttttt gtgaatccac tatttggctt aagtttgact ttaggagtgt
    10021 atgtattatc tgataaattt ttgcggaaat ataaactgcc tgttttgaat cctctcgtat
    10081 tatcgattgt tgttattatc ttatttttgc atttttctgg gatcagctat aaagactata
    10141 atatcggggg caattttttg actatgctga ttactcctgc gacagtggca cttgccatcc
    10201 ctctttataa aaacttccgt ttactgaaag agaattttta tcctgtaatg gctgcgattt
    10261 tagcaggaat agtcgcaaat tgcttggtta gtgtagggtt cggttacttc ttctctcttc
    10321 ataaagagat ggtgatctca ttactaccta aatctgtaac gacggctatt tctgtcgatt
    10381 tatctcatac gatgggcggg gtgaatgccg tgacacttgc tattgttgtc tctacaggta
    10441 tttttggttc gctgatcgcc actcatattt tccgcctatt caagatcgaa agtccagttg
    10501 ctcgcggtgt ggcgttagga agtacttctc atgctattgg tacagcaaaa gcaatcgaga
    10561 ttggcgaaat agaaggaatc atttccggac ttgcgatatg cgtcaacgga atattgaccg
    10621 ttttattgct accgttattc tttcagcctt ttgctggctt gttttgatgc gaaaaccact
    10681 attttatgct acaataacca aaacgttcga gcataaaaaa ggagggaaag acgtgtattt
    10741 gtcttccgag tatcttaaac aatttgataa agatctatat tataagattc tcctcctaga
    10801 aagttttgag gatcaagctt ggcataccgc tgcacaatta gcgcaagttg ttcagctgga
    10861 tgcccgcagc gtgtcgaaat atctgaacga gctgtctaaa aactatcagc agtttagcgg
    10921 taagacacac cctttgttta caaaaaatca ccgttctggt tataactttt atgatacgct
    10981 cgattcaatt gaacatgagc gttttttgat ctatctcgtg caaagtacgc tgaaattcca
    11041 gttgctgcac gacatttttt ttgaagaatt ccatacaatg taccaatttg cgcaaaagca
    11101 ttacattagt gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc aactgcaaac
    11161 ttacggaatt cggctgcaac gaggtacata tatcgctcaa ggcgaagaag aaatcattcg
    11221 actttacttg catatgacat tctggcagtt gtttcgaggg aaaatctggc catttgaaac
    11281 gatttctcaa atggacgtaa agaatatggc agaacatatc atggcttttt ttaatgttcg
    11341 tctgaacgag atcaaaaaaa gacgattgga atacatgcta ggtgcctttt ttttaagaaa
    11401 atcacagaag cattatgttg tattgaatga gaaaaaaaga cggctgattt ctgacaatct
    11461 gttgttccag cgattttgcc aagtcatgga gccggtattt cctaattatt ttcaagtaga
    11521 agacgagtta ggcgcattat ttcttgtact gatgactcgt gaagaatatt atagtgatcc
    11581 taagatacgg aagaaaattt ttgattttca tcaacaagca aaaacaccgc cctttacagc
    11641 cctttcagaa gcaaaagccg ctctttctct ttatcaggaa gagcaggggc tgccagcaga
    11701 aaatctaacc ttcgaggcag aaaactattt attcagcagt cattttttcg cctatctttt
    11761 tcctaatgcg aaagaaacga ttgatggcaa tagtagtgat tttattaatc atttagttat
    11821 tgaaaacaag gaattgaaac aatggctcgt acattttttt gaaagtagac acaaacatcc
    11881 caatcatctg gcatttaaaa atcatacgtt tttgatgggt cggtatctga ctgtgtttaa
    11941 aacattagga gcctttactc cacagcttcc aaagatcacg attcttttaa tgacagattt
    12001 cccattattt gaagagcaac tgttagagga gggattacgg acatttttcc gtaatgagta
    12061 tcagctgatt tttctgccaa cagattatcg ggggagagaa gttgatcttc tgatttcgac
    12121 aagtaaagtc caccgcaaac catgggcgga tcttgactat tttattgtga ccgaagaact
    12181 aaaattaatt gattacatcc aactatctca aaaatttgaa atgattcaaa aaaaaactat
    12241 taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta ccagaattga
    12301 aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat gttgttctcg
    12361 tatccgatga tgaatgtgat ggctgtccag tgacacctta cttattgatc aaaacaagtg
    12421 acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat gattgggcga
    12481 tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta tcacaggagc
    12541 tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg cttttgtatt
    12601 tggattagat caaaagttcg gtaaaatgga agaactctct ttgcgatttc ctgaaatttt
    12661 tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg tatcgaaatg
    12721 ccaggatcgt tttggtgaag ttacgatttt actgaataca gcggggatat tggatgccta
    12781 caaaccattg atggaaactg acgaatcgct gtgggatctg atttatgaga cgaatgtgaa
    12841 aagcatgtat cagctgacaa aaatcattct tcctgatatg atccgccaaa aaagcggaac
    12901 gatcatcaat atggcttcga ttgctggctt gattgctggt ggaggtggga tcgcttatac
    12961 aagcgcgaaa catgcgattg tcggatttac caaacaactc gctctggatt acgcaggaca
    13021 gggtatccga gtaaaaggaa ttgctccagg agccattcaa acaccaatga atgcggctga
    13081 ttttgaaggg aaaggtgaga tggcagagtg ggtggcaaat gaaacaccag tcaagcgatg
    13141 ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc aagcaagcta
    13201 catacaagga acgatcatcc caatcgacgg cggatggttg ttaaaataac cttgtgtttt
    13261 tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat aaaaaaagtc
    13321 gatgactgtt cccgacattc gatgccgaac agccagttca aaaaattctt tttaataatc
    13381 gttaatattc gaaactaagc ggcttttcac gaccttttga tataaacggt gttcaaaagc
    13441 ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata aggagctatt tttatttgac
    13501 tattcttatt acttgttgca gggcagcttt ttcactacct tctgcggcac ttcagttgga
    13561 attttcttcc catcctttgg tcgcggtcat taccaagata aaattatctt gcaaaggaga
    13621 aatagaacat ggaaaaaaat aatacccgtt cagccaaacg ctggacaacg acagatgtga
    13681 ctcggatggc gctagtcact ggtttatatg tggcagtgac gctggttttg tctgtcttta
    13741 gctttggagt gattcagatt cgtctctcgg agatgttcaa ctatctgtcc ttgtataaca
    13801 aaagatacat cgtcgcggta acagcgggag tagctttggc taatattgct tctcctcttg
    13861 gactgataga tgttgtagta ggaagcgtca gcacgctgct tgtattgctt atcaactata
    13921 aaatcactag tcgcatcaaa aacatgaaaa tcaaaatagc tgtgacagca tgcgtatttg
    13981 ctttttccat gtttacagta gctggtcagc taacgatttt atatcaacta ccttttttct
    14041 tgaattggtg ggtgattgct ttaggagaat tggcatcaat gattgtcggt gggataatta
    14101 tttattggat cggtaaaaaa gtggatttgt caaaataaag atctgtttaa tttaaaatat
    14161 ggcagcatgg aagaagactt tgagtcttct tccatgctgc ttttttgatt aatttgtatg
    14221 aagaaatcga tttagccgtt ttttcatcaa gataccgata taagctgctc cagttgcctt
    14281 gattgcttcc ggcagtaaaa acggcagtag tccactggca aatgcagttg taaaagtaag
    14341 atcaccactt attttcagcc agatcgttcc gcagatcaat gcagcaagag agcccattag
    14401 attggcaaag attgcataca agtaatgagc atccgttctt tcgatcatcc aaccagttat
    14461 ccacgtttgt aagagaaatc caatcaaata tccgccagtt ggcccaaata aaacagctat
    14521 acctccagtc atccctgaaa atacaggaag accaatcaag ccaagtaaaa gataaactgc
    14581 aacagatatc aatcctagtt ttcttccgag aaaagttgct gttaatccaa tagcaaatgt
    14641 ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc tgagaaagga cagaaataat
    14701 cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt cggttcataa cgccctcttt
    14761 tcgttaacta aatattttta tttggttaac gaaattgtaa aacaaagtga ttactttgta
    14821 aatagaaaat ataaaaaaag accagctatc caatagatag cggtcttttt caagaaagct
    14881 tttgaaatta taagctttgg ttgtagtatt caacgacaag agcttcgtcg atttctggat
    14941 atagttcgtc acgttctggt aaacgagtca atgaaccttc caatttttca gcgtcaaagc
    15001 ttacgaatgc tgggcgtcca acagttgctt ctacagcttc ttttactgtt acgatgtttt
    15061 gtgatttttc acgaactgag atcacttgac ccacttctac gtggtatgaa gggatatcaa
    15121 cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa ttgacgtgct tgacgacgag
    15181 tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg ttgttctagt aagatcatga
    15241 agttaacacc atgtttacct tctttgattt tgctagcttt tacgaacaag ttacggaatt
    15301 gacgttcgtt cattccgtac atgtgacgaa gtttttgttt ttcagttaat tgcatgccgt
    15361 attctgattg tttgccacgg ctgttaggtc catgttgacc tggtgcgtaa gggcgacgtg
    15421 ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg acgagaaatt ttccatgatg
    15481 gtcctgtata acgagacatt aaaatttcct ccaatgaata taagtttttg gagtaaaata
    15541 atccgttgaa aaattcataa tcatatagtt tgttcttcaa tcttcacctt tgcagccgcg
    15601 gttacacaat cgaacccgag acggggcaac aaactaggga tgagttatta tttttctgct
    15661 gcattatttt acacaaacta cagtatatga tagatcatcc atgaatgtca agataattag
    15721 tacataaaaa cgctttacaa acctaggctg tctttataaa atgagagggt atgtgcaaat
    15781 ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat ccggtatcac cattatttga
    15841 gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt gaaaacagct tttcacgtta
    15901 cgctgttcga gcggttctcg cctgtttgtt tttgacttta ggaacggctg tggcatttgc
    15961 gatcgcgatg aagggggaag atatcgcgta tggtctagga aagattttat acgcatttat
    16021 gttcagctgg ggtctggtca tgattctata catgaacgcc gaattaggaa catcgaatat
    16081 gctttatatg accgtgggtg tttttcggca gagacttgct ttaagtaagg cagccaagat
    16141 tctatttgcc tgtatttttt tcaatatgat tggcggtata ttatgtggat ttctaatttc
    16201 tatgactacg ccatttcaag atttaacgtt ggacaacttt atgttagaat caatagcagt
    16261 aaaactagca aaacctgcag caacgatttt ggtggaaggg atgtttgcga atatcgttgt
    16321 caataccgct gtactgatca gcatgcggat gaaagaggat gccggaaaag tgatcacagt
    16381 cgtattcatc attttcattt ttgctttttt agggtatgaa cacgtgatag cgaattttcc
    16441 tgcctttacg cttgcttact ttgcttctca cggacagatg gatggaatga cagtcagcaa
    16501 tgtgttacat aatttgtttt ttgctttggc aggaaattat attggtggcg gtttagtaat
    16561 gggactggga tatgcttggc tgaatcagtc aaaatcatcc tatgtggact aaaaagtcgt
    16621 tcattaaagg ggaagtgcca tggaagtttt agtttttcca aaaaatgagg aaaaagcgtt
    16681 acaaatggaa aagtacatga aaaatctttt ttcctttgct ggagtaccta agccggaacg
    16741 cgctctttta caacaaccgt tactggatga gagcaaaaat ttatctattt ctgaagtttt
    16801 agcttttgtt cattattatt atgaacaaat cgaaagagag tatcagtatg tagcgatcga
    16861 tttagtccag aaaaacatca aacggcttac atttgaagac ttagtcgtat tgttgacact
    16921 agttcagaaa aaagaatggt gggacagcat cgactcttgg agaaaagtat acagcacatg
    16981 gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa ttgtttgaaa aaaaggaaga
    17041 tttttggcta cgtcgaatag caatcacgtt gcaattaggt tataaacagc aaacagacac
    17101 aggactattg atccgagcaa tagacgaaga tcgtcaaaca aaggaattct ttatccaaaa
    17161 agcaattggt tgggcattac gcgattatag taagaccgat gccgattggg taaaaagaca
    17221 gctgacaaca gatttgagta agttagctgt tcgagaggca agtaagtatt tataagaaag
    17281 cgagacgaca agagatgaga cgcaaattat ttgcttttga tattgatgga acgttgttag
    17341 attcaaacaa acaagcttta gatagcacga gagaagcatt ggaaaaatta cgcaaagccg
    17401 gtcatctggt cacgattgct acagggcgga gccgatttca tgcgcaagag atcattcgcg
    17461 atcttgattt tacgaactac attttatgta atggagcggc cggtttttta gaccatgaac
    17521 aagtatataa gaatttacta gatcgcgacc agttggatgc gttcgttcaa gaagcgcatc
    17581 atgagcagat cgatacagct tttgtcagct tagacaatat caagcgttct tcttccaatg
    17641 atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc tgtcttgcct gaattagatc
    17701 agttttttgc agaggaacaa gaaatatacc aagcgttagc atttttcgat caaacatatg
    17761 atgggaagtt ctcttcatat gataaaattc gatttgtccg ctggcatgag aacagtgtag
    17821 atgtcgtacc taaaaatggt tctaaagcag cgacaattct gcacttagca gaacaagtag
    17881 gcatccctca taaagacatc atcagctttg gcgatggaca aaacgaccgt gaaatgttgc
    17941 gcatgtcagg aattgggata gctatgggga atgctgtgcc agaagtacaa gcagaagcta
    18001 agatggtcac tgatacaaac gatcaggatg gaatttggaa ggcattgaaa gagttaagcg
    18061 tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag taataagccc agccaaacaa
    18121 aaatagctcc tcctattttt cgtttttctg ggcttattac cgcagatgca agcttttgaa
    18181 caccgtttaa ggaagaggtc gtgacagaag tggtcagttt caagaaataa gaagggattt
    18241 acgaaaattg cttctcaaat ttttgtaaaa ttacggctta tttctgaaga agtagatgaa
    18301 acaacgttta ttcgggaaat gggactgtga caagatgttt gtcatagtcc cattttttta
    18361 ttttattttt caaaactaag aaataccagg aaagagttcc ttttgtttga ccttttcttt
    18421 cttattgaaa acaactataa tgtagagtga tgttaaaaga gaagaggaat ttagtgtgcg
    18481 ggtattgttt attggtgatg tagtaggttc aatgggacga gagatgatta ctgaatatct
    18541 tcctagattg aagaagaagt accgtccgca agtaacgatt gtaaatggtg agaatgccgc
    18601 ttctggacgt ggaatcacag aaaaaatcta taaaaaattt ttgcaagacg gcgtagatgt
    18661 cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc tttgagtttg taaatgaagc
    18721 taaaaagatg gttcgtccgg caaattttcc ggaagggacg cctggacaag ggatggtctt
    18781 tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg caggctcgtt cttttatggt
    18841 ggacttggat gatccttttc gtaaaatgaa tgagctggta gaagaagcca gaaaacgtac
    18901 gccgatcatt tttgtagatt ttcatggaga aacaacaagt gaaaaacaag caatgggctg
    18961 gtttttagat gggaaagttt cagcagtggt cggtacacat acacacgttc agacaaatga
    19021 tgcgcgaatt ttgccaagag gaacagcgta tctgacagac gttgggatga caggtcccta
    19081 tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa aagtttctga ccgcattacc
    19141 aaaacgtttt gaagttgtgg aacaaggccg atcgatttta agcggctgta tcttggaatt
    19201 ggatgatacg acaggtcatg caaaagaaat ccagctgatt cagatcaacg atgatcgacc
    19261 gttcatggag tagaaagaga ggcagttttt tgcagcagga gccggaaatt caaaaagaag
    19321 taaggaaact gacaaaactt ttaagagaga atgaaacgat cattcgctac aaagaacttg
    19381 aagaaaagat ccagcaaaac cagtatttag ctgagttgag agaaaaaatc aaacaagcgc
    19441 aaaaagatgc tgttcatttt gcccactatg acaaaccagc agcagaacaa gaagcaatca
    19501 aacaagcgga tcaattcatg caagaatttg atcagcatcc gcttgttgta gcttatagaa
    19561 aacagctgtt ggaagcagat gatctgcttc atcatttaac aactatgata caagaagaaa
    19621 tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa ctaaaaatac tcctatgatg
    19681 gaacaatatc taagcatcaa ggcccaatac caagatgctt ttttgttcta tcgtttagga
    19741 gacttttatg aactatttta cgaagatgcg atcaaagcgt cacagctttt ggaattaacg
    19801 ttgacgagca gaaatcgaaa tgcagaagaa cctattccta tgtgcggtgt acctcaccat
    19861 gcagcacaag gatatatcga tgccttgatt gaaaaaggct ataaagtagc gatttgcgag
    19921 caggtagaag atccaaagac gactaaaggg atggtcaagc gcgaagtgat ccagctagtc
    19981 actcctggta cagtcatgga cagcaaagga ttgtcggcaa aagacaacaa ttttttaaca
    20041 gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg ccgatttaag tacgggagaa
    20101 ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa atgaagcctc tgcattgcag
    20161 acaaaagaac tcgtattagg cagtgagatc actgataccc ttcgtgaaca gctaagcggg
    20221 cgtttagggc tagtcttttc tcaacaaaat gagatggaag aaaacgcgga atttagcttt
    20281 ttgacaagcg aactagtcga tccgctggaa aaagaagcaa ccggcaaact attgacttat
    20341 ttatcggtga cacaaaagcg gagtttagct catatccaaa aagcagtaga ataccaacca
    20401 gatcattttt tgaaaatgga ttattattca aaattcaacc tagaattaag ccaatctatt
    20461 cggactggga aaaagcacgg cactcttcta tggttgttag atgaaacaaa aacagcaatg
    20521 ggtggccgtt tactaaaaca atggctagac cgtccgctta tccaggcaaa acaaattaaa
    20581 gcaagacaag aaatggtcgc ttctttgcta gacagctatt ttgaacgtat cgatttgcag
    20641 tcagcattaa cgaaagtcta tgatctggaa cgcctagcag gtcgagtagc tttcggtaat
    20701 gtcaatgggc gagacttgat ccagctgaaa acttctttag aacaagtgcc aatgatcaga
    20761 gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc tgctttcaga aatgcagccg
    20821 atggaccatc tcgtccaatt gatcgaccaa gctatccaag atgatccgcc gttacaaata
    20881 acggaaggaa acatcatcaa agatggattc aatgagcagc tcgatatgta ccgttctgct
    20941 atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa aagagcgtca agaaacagga
    21001 atcaagaact taaaaatcgg atttaaccgc gttttcggtt attatataga aattacaaaa
    21061 gcaaatcttg gaaatgcaga actggagaag tatgaacgaa aacaaacttt agcaaatgca
    21121 gagcgtttca tcacaccaga actaaaagaa ttggagacac aaatcctcga agcagaagaa
    21181 aaatctgttg atttagaata tcagttgttt ttagctgtga gagaagaagt caaaaaggct
    21241 atccagccgc tacaagtact tgcaaaagca atcagtgcag cagatgtgct acaaagtttt
    21301 gcaacgatca gcgaacgcta tcagtatgtt cggccagaac ttgtcagtga caagcatcag
    21361 cttctgatca aagacggacg acatcctgta gtagaaaaag tattaggaca tcaggaatat
    21421 atccctaaca gcgtagaaat ggcagaagac gaaatgatct tgctgattac aggaccgaat
    21481 atgtctggta aaagtacgta tatgcgacag ttggcattaa ctgttctgat ggcgcaaatg
    21541 ggctgtttcg ttccggcaaa acaagcagtt ttacctattt ttgatcggat ttttacccga
    21601 atcggtgcaa gtgacgactt aattgctgga caaagtactt ttatggtaga gatgatggaa
    21661 gccaatcaag ccctgcgtta tgctacgcca aacagtttga ttttgtttga cgaactaggg
    21721 cgaggaacag caacctatga cggaatggcg ttggcccaag caatcatcga gtatatccat
    21781 cgtcatgtac aagcaaaaac actcttttct acccattatc atgaactgac ggtacttgag
    21841 aaagaacttc cgcagcttaa aaatgtccat gttggagccg ttgaaaaaga cggagaagtt
    21901 gtcttcttgc ataaaatgat ggatggacct gcggataaaa gctacggcat acatgtggca
    21961 aagatcgcag gattgccttc caatctacta gaacgagcag cagatatcct gcatacgcta
    22021 gaaaatggcg ataacggaca ccatactgtt ccagctgaag tagaagagaa aactaaaaca
    22081 gccaaacagg agcaaataaa accacctgca gaaaaagtaa atcaagacca tatacaagac
    22141 cgtgtaaaag aagaaaacca acagctttct ttattcagtg aattatccga aaatgaaaca
    22201 gaagtcattg aatcactgaa gcaaataaat ttactagaaa tgacgccaat ggatgcacta
    22261 aacacattat atgaattaca aaaacaactg taggaagaag gcggatatcg atggccaaaa
    22321 tccaagaatt atctgaacgt ttagctaatc agatcgctgc gggagaagta gtggagcgac
    22381 cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga cgccggcagt actcagattg
    22441 atatcttagt tgaagaagcg ggactgcgga cgatccaagt gattgataac ggagaaggca
    22501 ttttggcaga cgacgtagag aatgccttca agaggcatgc gacaagtaag atccataatc
    22561 gcgacgatct tttccgcata cgaacgcttg gatttcgcgg tgaagctttg ccaagtattg
    22621 cctcggtttc tgaaatgact ctggaaacgg caaatcagga agaaaaacaa ggcacctatc
    22681 tgtcgctgaa aggcggagaa gttattgagc atcgaccagc accactgcgt caaggaacga
    22741 agattaccgt cagcaatctg tttttcaata cgccagcccg cttgaaatac gtgaaaacac
    22801 tgcacacaga attagcgaac atcggggaca tcgtcaatcg actggcattg agtcatccat
    22861 cgattgcctt tcgtttagta catgatggaa acaaaatgct ggcaactgct ggaaatggcg
    22921 atctgaaaca aacaattgcg ggaatctacg gattatctac cgcaaaaaaa atgctgaaaa
    22981 tcgaaggaaa agatctagat tttgagttat ttggctatgt ttcattgcct gaggtgactc
    23041 gtgccagtag gaattactta tcgacgatca tcaatggacg ttttatcaag aacttttcct
    23101 tgaataaagc aatcgttgca ggttatgggt caaaattaat ggtcggtcgt ttccctattg
    23161 ctgtattgga aatcaaaatg gacccgcttt tagtagatgt aaatgttcat ccgacgaagc
    23221 aggaagtccg gctgtcgaag gaaaaagaac taacagagct aattagtaac gccattcaaa
    23281 aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga taatctacgg tttaagaaaa
    23341 aagtacccga tcagcctaaa acagaacaaa tcgaaatcga tttgtcctac gcttttgaag
    23401 aagaaaaacc taaaaagcca agtagtttga attttgatcg agagacagga acctttttcg
    23461 tagaagaacc aaagaacaca tttgttgaca gaattgttga taagtctgtg gaaaatgttg
    23521 aaaactattc tggtttttct gaagaaaaca ccacttatcc agaaaaaaca gcgaaaacgg
    23581 tggataagtt tggtgaaaat gttgataact ttgtagaaaa aacagtggaa aaatctgaaa
    23641 agaaagaatt tcccattgtg gataacatgg agaaaacaaa tgaagaacaa gcagataaat
    23701 tcgtagaaga ggatgaaaag atttacgagg aagaattggc gctccatcct gaatttgatt
    23761 tcactcaagg aaacgacaaa acattagaac aagccatcaa gaaattagaa acggaaaaac
    23821 cagtacaacg ttttcctcag ttggaatatt ttggacagat gcacggaacg tatctttttg
    23881 cccaaagtag cgatgggttg tacatcattg atcagcatgc tgcccaagag cgcattaagt
    23941 atgaatattt ccgggaaaaa attggggaag tatctgatga tctgcaagaa ttgcttgttc
    24001 catttgtgtt agactatcca aacagtgatg ctttgaaatt aaaagagcaa aaacacttgc
    24061 tagaagatgc agggatttat ttagaagaat ttggtcaaaa cagctttatt gtacgggccc
    24121 atcctacttg gtatcccaat ggagaagaag aatcgattat tcgggagatg atcgatatgc
    24181 tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga agcaacagca atcatgatga
    24241 gttgcaaacg atcgattaaa gccaaccatt atttgaatga agcacaagca cgtgtgctgc
    24301 tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc ccatggccgt ccagtattga
    24361 ttcactttac caattcggat atggaaagga tgttcaaacg aatccaagac ccgcattaag
    24421 tcaaagagaa gagggaaaac atgaagattg tattagcatc ccaatcacca agaagaaaag
    24481 agttattggc tcatatcgtt tcagaattcg agattgaacc tgctgatatc gatgaaacgc
    24541 catttccaga cgaacagccc gtcgattatg ttcgtagaat ggctgaagaa aaagcacgaa
    24601 ctgtctggga acaagatgaa aaagaagacc atttagtgat tgccagtgac acgactgttg
    24661 ttctcaatca agagatcatg ggtaaaccag aagatctggc tgaggctgaa ttcatgctga
    24721 aaaaactaag cggggaaaca catactgtat atacagctgt tgttttaaaa acaaaggaac
    24781 gagaagaacg gatactagca gaagcacatg tcacttttta tcctttgacc gacgaagaga
    24841 tcaaacgcta cttggagacg ggggattatg cggataaggc aggggcatac ggcatccaaa
    24901 atgctgcgag tgtattcgtc aaagaaattt ctggagatta ttacagtatc gtaggatttc
    24961 cgattggtgc tgtcaatcag gcactgaaaa cattcattta aacaaaaaag cggactatct
    25021 gattttcgat tgaaaatcca tgatagcccg ttttttttat tgaaaagatt acttatctac
    25081 tctgactaag attttacttc agctttaccg actgcttcgc ccgggcgaac aagtcctgat
    25141 tggtcaagag aaagtcctgc aactttttca ttattggtca aagcaacaat aatgtcagtt
    25201 ttcttgccag cttgttctat ttctggcaaa tccatttcag caatcagggt ctctggtgtg
    25261 acaaggtatc cttctttcac gaatacattg aaaggaacac cctttagttc aacagtatct
    25321 aatcccatgt gaacaagtac ttccagcccc tcattagaaa gaataccaat ggcatgtttt
    25381 gtttcaaaga cactcgtcac tttaccgttt actggtgcat agactttgcc gtttgacggt
    25441 tcgacagcat aaccgtcacc catcattttt tgtgagaaaa ctgggtcatt tacttcagtt
    25501 atttccttga tggtccctgc agcgactgcg aataatgttt cttttgttgc aactgccgta
    25561 ccagttgctg aaacaggttc gaaagactgt ggcgttgctg ttgcgccatt tgctgtttga
    25621 agttgtacat cagcagcatt ttcaggaaca gcaggatcaa tcttattcaa aattccctga
    25681 cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag caatgatcat tgcgatgatg
    25741 aaaggtaccc atgtttctgc ttggatagct agtattcctg gcaaaccgcc tactccgatc
    25801 gcgttagctc gaacacccat caagttagca aacatgccag ctaatccaga gccgaccatt
    25861 gcagcgacga aaggatagac gtatttcaaa ttgattccga acatagcagg ttcagttacg
    25921 cctaagtaac aagaaatcat tgctggaatc gagatttgtt cttctttttt attcccacga
    25981 tgtaagaaga tgatggctaa gactgcagag ccttgggcga tatttgataa agcaatcatc
    26041 ggccataaat tcgttgatcc gaaatctgca atcagctgca tatcgatggc attggtcata
    26101 tggtggagac cagtaatcac taaaggagca tacaggaatc cgaatacggc actaaacaac
    26161 cagctgatag aagacgtcaa tccagtattt acgatatttg agatccagct tccgactgtc
    26221 cagccgatag gacctaaaat aacgtgtgca gctaaaacag ttggtaacaa tgaaaacaat
    26281 ggaacaaaaa tcatcgagat cgactgaggg atatatttgc ggaagaagat ttctagatac
    26341 gctaacataa accctgccag catggctgga ataacttgcg cttgataacc aatcatctgg
    26401 acttgagcaa aaccaaaatc ccaaaatgga atatctgctg cggctgtgga agctacacta
    26461 taagcattta ataattgcgg agaaactaac gtaattccta aaacgattcc taatatttgt
    26521 gtagtaccca tcttttttgc aatactccaa gtgataccta ctggaagaaa atggaaaatt
    26581 gcttcacccg gtaaccacaa aaaggcattg acaccattcc aaaacttcga gtgttcaacg
    26641 attgttccac caagactttc aaactggata ccttcaagta catttctgaa tccaaggatc
    26701 aatccaccga caatgattgc aggaatcagt ggtgtaaaga tttctgctaa aacggcaata
    26761 gcacgttgaa cgggatgaag gttttgtttt gcagctgctt ttccttgttc tttggaaacc
    26821 ccttcaactc cagatactgc aacgaagtcg ttataaaaag tagaaacatc gtttcctatg
    26881 atgacttgga actgtcctgc attcgtgaac atccctttta cgctaggtat atcttcgatc
    26941 gctttttcgt ccgcttttcc tggatcattc aggacaaaac gcatacgagt cgcacaatga
    27001 ctaactgcag caatattttc ttttccgccg atatcatgta acagcttctc ggcatctgct
    27061 tgatattttg ccatatcaat aaaccccttt ctcttaactt gtctatgcaa gttgtttctt
    27121 catttactat actttttttc ggagagaatt gcaagcgttt tattaaaaag tacgagagaa
    27181 aatgatatcg ttttaaaaag cttaaaataa gcttttctta aaaataggaa cattgaaata
    27241 caaaaagagc tatagtatag tgaagaaatg atggaagaac gagtgaggga aaaaacatga
    27301 ataagttcaa tgaaattttt ttagatttgg aacagaaaat tttagcgaaa gaatatcctc
    27361 cgcattcgct tttacccagt gagaaccaat tgatcaaaat ttatggtgtt tcacgagaaa
    27421 caattagaaa agcgctgaat cttttgacta cggaaggtta tattcaaaaa aaacaaggaa
    27481 aaggttcgat cgtactggat cggaatcgct tcgactttcc tatttctgga ttgactagct
    27541 ataaagagct acaggagacc caacgtattc caagtgaaac gatcatccat acgttagaag
    27601 aaacagaagt gacaaaagca ttgaacgaga tcactggctg ggagatcggt gcgcctgttt
    27661 ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt tatattagac accgattatc
    27721 tattaaagga aatcgttcct attttaactc cagcacaagc acaaggatcg atctatgaat
    27781 attttgaaaa tgagctttct ttgacgattg cttacgcgca aaaggagatc acagtagaag
    27841 aagtaacgga aatcatgaaa acagcgatgg atcttcacga tgacacttat gcagtagtag
    27901 tcagaagcct cgttcatctg gaagataccc gctgtttcga gtattctgag tcggttcacc
    27961 gattagataa attccgtttc attgagtttg cccgacgccg aaaagtgtaa atagttcaaa
    28021 agaaagcaaa ttgttggcat ctgtccactt tggcgctatg ataggatcac aaagggggct
    28081 tttttaatgg ataaaccaat aagaccagaa aaagaagaac tgaaagaaaa gttatcaccc
    28141 atggcttatg cagttacaca agaaaatgca acagaacgac catttagcgg gaaatatgat
    28201 gatttttacg aaaaggggat ctatgtagat atcgtcagcg gtgaaccgtt attcagctct
    28261 gcagaaaaat atgatgcagg ttgtggctgg ccatcattca gcaaaccaat aacgaaaaga
    28321 caagtgcgcg aaaaagccga tttttctcat ggcatgcatc gaatagaagt tcgaagcaaa
    28381 gaagcagatt ctcatctcgg acatgtgttt accgatggac cagtagatca aggtggtcta
    28441 cgttactgca tcaattctgc ctcactgaaa ttcattccat atgaaaagat ggatgaatta
    28501 ggttatgggg aattcaaatc acttgtagaa taatggaaat acacaaagag cggtacaata
    28561 cggggattta ctcgatcgtc cgctcttttt ttattcaagc gattgctatt tgaaagaaac
    28621 gctccccaga ctcctttttt cacaaggtga aatgtgctac aataaaggga acgtatgtgt
    28681 gcaaaagaaa gaggactaga atatgtatga atatctgaca ggtgtggtta cctttatcaa
    28741 cccctattac atagtagtag aaaccaatgg tatcggctac cagatcgcac taggaaatcc
    28801 ttaccgctac agtagcaaac taaatcaaaa aataaaaatt tatgtccatc aggtgatacg
    28861 tgaagatgcg catcttctat atggatttga ttcgctagaa gaaaagcaac tgtttttgcg
    28921 attagtaagt gtttcaggaa tcggtccgaa aagtgccctt gcaatcatgg caagcgaaga
    28981 ccactccggt ttgattcaag cagtagaatc cagtgatgtc acttatttga cgaaatttcc
    29041 aggagtaggg aaaaagacag cacaacaaat gatcttggat ctaaaaggaa aattcggtga
    29101 gctgagtatc gatacacctt ttagtctatt tgatgaagca aacacaaaag acgctacagc
    29161 tctatctgaa gcaatggaag ctctctcggc tcttggttac agtgatcgag aaatcaaacg
    29221 tgtagaaaaa caattaaaag aagtcgataa tcagacaacg gatgagtatc tacgccaagc
    29281 actcaagcta atgatgaaaa aataaggagg agaccaaatg acagatgaat acttgttatc
    29341 accagaatct ggcgaaaatg aactgagttt ggaaaaatct cttcgaccac agctgctttc
    29401 tcaatatatc ggacagagaa aagtcaaaca agaacttcag atttacatcg aagctgccag
    29461 aaatcgggaa gaacctttag atcatgttct gttgtacggg cctcctggat taggaaaaac
    29521 gacgatggcc atggtcattg ctaatgagat gaccgtaaat attcgtacga cgagtggccc
    29581 tgctatcgaa cgtccgggtg acttagtagc aatcttgaat gaactggaag caggggatgt
    29641 cctgtttatc gatgagatcc atcgattacc tagagtggta gaagagatgc tttattcagc
    29701 tatggaagat ttctatgtag atatcatggt aggacaaggg ccaaccgcac atccggttca
    29761 tttccctctt cctcccttta ctttgattgg cgcgacgaca cgggcaggta tgctttctgc
    29821 acctttacga gatcgatttg gcatcatttc tcatatggaa tattatgacg aacaggatct
    29881 gcaggagatc gttttacgtt cttcggatat ttttcaaaca gagatcattg aagaaggggc
    29941 attggaaatt gctcgccgat cacgaggaac gcctagaatt gctaatcgct tgctcaaacg
    30001 ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc gatcgtccta tagcggatca
    30061 ggcacttact ttgctacagg tcgatcaggc tggattggat tatgttgacc agaaattgtt
    30121 gcgaacaatg atcgagttat atggcggcgg accagtaggg ttaagtacgc tttcagtaaa
    30181 cataggagaa gagcgtgaga cggtagaaga tatgtatgaa ccgtatctga tccaaaaagg
    30241 atttttaaag cgtacaccta gagggcgaat tgctactgct tatgcctatg aacattttgg
    30301 ttacgactat catatatgat ttttgctttt agattggagg aaaatgaatg agtgatacaa
    30361 cacgtacgaa acaattgatc aaaaacgtat tgattgattt gtgccaaacg caatcttttc
    30421 gaaaaatcag cgtccaaaaa atcgcaaatg aagcaaaaat caatcgacaa acattttatt
    30481 atcattttac agataaatac gatttgcttc gttgggtcta ttgtgaggat gcattgcatt
    30541 atttagatcc acaatgtctt tcacttgata attgggaaga gcaagcattg aaaatgctga
    30601 aagcaattgc tgagaagaaa gaattttatg caaatactgt gctttctgac ccagaaattt
    30661 tacaaaaaga gttttctcaa ctgatccaaa aatcatttat caagattttt gaacaaatgg
    30721 atgaagaaag acaactttct gcaagagaca aactatttta tgctcgtttt ttttcttacg
    30781 gttgcagtgg tgtattagtc aattggatca cgaatgaata tgaagaaaca ccgatggaaa
    30841 tcgctatgca gcttttccgt ttagccaagg atacggaatt atattcttat cgtctttatg
    30901 caaaagaaga gttggaagaa cagtaataga aaaatttttt gttggaaaag caatgatcgc
    30961 taaaaggaga tggggaaatg acaaaaattt tatttgtttg tttgggaaat atttgccgtt
    31021 caccgatggc agaaggcttg ctgagaaaac gaatagcatt agaaggaagg gaagcagaat
    31081 tttttgtgga ttctgctgct acaagcacgt atgaagttgg aaagacaccg caccctggaa
    31141 cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa tatggttgct cggcaaatca
    31201 caccccacga ttttgaaact tttgattgga tcattggcat ggatcaagaa aatgtagaag
    31261 aactgaaacg aagagcacca cgctcagcgc aggggaaaat ccatttgttt ttaagtagca
    31321 ttcctggaaa agaaaaagaa aatgttcccg acccatacta taccaataac tttgatgaga
    31381 catatcattt gatcacagaa ggcctgaatc attggtggaa tatttggatt ggcgaaaata
    31441 aaataacaaa caatttgtaa agttgacgct ttctttttaa ggcgatgtta gagcatttcc
    31501 ttgttttttt gtaaaatgtt aggtaaaata aaattaatta agtaacactt tcttagctaa
    31561 aggaggagtg cattcgatga acgaagagac agtatcgcca atgaagaagc ttacgaagtt
    31621 agttactcat acttcgtatc aactttttga ttcttttgta aatgacactg aagaacatgc
    31681 taagaatgta cgattgcaag gaaagtttat tcttgagctg caaaaagctg ctactgaaca
    31741 tagtctagtt atcttacaag taagagcaaa tcgtttgagt agcaagtatg aaacgatctc
    31801 aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg atgatccgcc tacaaaacga
    31861 agagcagcag atccggatgg ttcctgtaga acaaattgaa aaaataacaa tcctttcacc
    31921 agcaggggaa caggaacgat tatcaaaata atgtatacca gatagaaaag cacgtatgag
    31981 cttgtaccat caatcattac taatcaaaaa taaccgaatg ctttaagctg atatcagtaa
    32041 aacggaacgt tttaggatga gtatcacagc atgacagcat tcggttgatt agttgaatcg
    32101 attggatggt acaagttttt ttgataaaaa aaggtcctgc tagccgggga gctagcagga
    32161 taaggagtta aaaatgaaaa agtgttgtta gggttgtttg ttggtatgct tatataatag
    32221 tcattagttg tgaatatttt gtgacgaaag ttttcgaaag ttgttaagtt tttttgcgaa
    32281 aactattttt atatcatata tatgtctaaa atctatatta taaaagaaat gttgtttgat
    32341 cagcttctct tcggtattag ttcaaaacaa aacggcaaag tatatggagc tactttgccg
    32401 ttttgtttct ctagtactca ggtcagttta tagacgctca ataaacggtg ttcaaaagcc
    32461 agtttctcgg tattaagtca aatttatgca aaaatgtgag aagccatttt cacaaaatat
    32521 ttcttaatac ttgaaactaa gcggcttttt cac
//
LOCUS       DACCSK010000018        31581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCSK010000018 DACCSK010000000
VERSION     DACCSK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.018"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(76..543)
                     /locus_tag="IVP39_000757"
     CDS             complement(76..543)
                     /locus_tag="IVP39_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7585848.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IVP39_000758"
     CDS             complement(663..2201)
                     /locus_tag="IVP39_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7585849.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IVP39_000759"
     CDS             2281..2967
                     /locus_tag="IVP39_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7585850.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IVP39_000760"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IVP39_000760"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7585851.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IVP39_000761"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IVP39_000761"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7585852.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IVP39_000762"
     CDS             complement(4453..5583)
                     /locus_tag="IVP39_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7585853.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IVP39_000763"
     CDS             complement(5583..7766)
                     /locus_tag="IVP39_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7585854.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IVP39_000764"
     CDS             complement(7763..8788)
                     /locus_tag="IVP39_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7585855.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IVP39_000765"
     CDS             complement(8833..9660)
                     /locus_tag="IVP39_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7585856.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IVP39_000766"
     CDS             complement(9657..10724)
                     /locus_tag="IVP39_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7585857.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IVP39_000767"
     CDS             complement(10750..11586)
                     /locus_tag="IVP39_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7585858.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IVP39_000768"
     CDS             complement(11583..12452)
                     /locus_tag="IVP39_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7585859.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IVP39_000769"
     CDS             complement(12522..13805)
                     /locus_tag="IVP39_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7585860.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IVP39_000770"
     CDS             complement(13819..15504)
                     /locus_tag="IVP39_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7585861.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IVP39_000771"
     CDS             15779..16804
                     /locus_tag="IVP39_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7585862.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IVP39_000772"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IVP39_000772"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7585863.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IVP39_000773"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IVP39_000773"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7585864.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IVP39_000774"
     CDS             25476..26444
                     /locus_tag="IVP39_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7585865.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IVP39_000775"
     CDS             26462..27367
                     /locus_tag="IVP39_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585866.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IVP39_000776"
     CDS             27364..28170
                     /locus_tag="IVP39_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7585867.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="IVP39_000777"
     CDS             28327..29295
                     /locus_tag="IVP39_000777"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7585868.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="IVP39_000778"
     CDS             complement(29612..30136)
                     /locus_tag="IVP39_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7585869.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31581
                     /gene="scm"
                     /locus_tag="IVP39_000779"
     CDS             30423..>31581
                     /gene="scm"
                     /locus_tag="IVP39_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7585870.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTT"
BASE COUNT         8867 a         6828 c         5945 g         9941 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gattttcgtg ttgtctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttcg ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    31501 gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca agcgacagta
    31561 cagatactac ctctaccaca a
//
LOCUS       DACCSK010000019        31156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCSK010000019 DACCSK010000000
VERSION     DACCSK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.019"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..440)
                     /locus_tag="IVP39_000780"
     CDS             complement(<1..440)
                     /locus_tag="IVP39_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HAP7585871.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVII"
     gene            complement(530..1075)
                     /gene="hpt"
                     /locus_tag="IVP39_000781"
     CDS             complement(530..1075)
                     /gene="hpt"
                     /locus_tag="IVP39_000781"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7585872.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(1075..2520)
                     /gene="tilS"
                     /locus_tag="IVP39_000782"
     CDS             complement(1075..2520)
                     /gene="tilS"
                     /locus_tag="IVP39_000782"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7585873.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(2640..3110)
                     /locus_tag="IVP39_000783"
     CDS             complement(2640..3110)
                     /locus_tag="IVP39_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7585874.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(3156..3581)
                     /locus_tag="IVP39_000784"
     CDS             complement(3156..3581)
                     /locus_tag="IVP39_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7585875.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(3648..3917)
                     /locus_tag="IVP39_000785"
     CDS             complement(3648..3917)
                     /locus_tag="IVP39_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7585876.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(3928..5523)
                     /locus_tag="IVP39_000786"
     CDS             complement(3928..5523)
                     /locus_tag="IVP39_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7585877.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(5538..9059)
                     /gene="mfd"
                     /locus_tag="IVP39_000787"
     CDS             complement(5538..9059)
                     /gene="mfd"
                     /locus_tag="IVP39_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7585878.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(9076..9636)
                     /locus_tag="IVP39_000788"
     CDS             complement(9076..9636)
                     /locus_tag="IVP39_000788"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7585879.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            9989..10963
                     /locus_tag="IVP39_000789"
     CDS             9989..10963
                     /locus_tag="IVP39_000789"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7585880.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            11133..12533
                     /locus_tag="IVP39_000790"
     CDS             11133..12533
                     /locus_tag="IVP39_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7585881.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(12611..13333)
                     /locus_tag="IVP39_000791"
     CDS             complement(12611..13333)
                     /locus_tag="IVP39_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7585882.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(13412..13918)
                     /locus_tag="IVP39_000792"
     CDS             complement(13412..13918)
                     /locus_tag="IVP39_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7585883.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            14041..15840
                     /locus_tag="IVP39_000793"
     CDS             14041..15840
                     /locus_tag="IVP39_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7585884.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(15908..17482)
                     /locus_tag="IVP39_000794"
     CDS             complement(15908..17482)
                     /locus_tag="IVP39_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585885.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(17737..18246)
                     /gene="mreD"
                     /locus_tag="IVP39_000795"
     CDS             complement(17737..18246)
                     /gene="mreD"
                     /locus_tag="IVP39_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7585886.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(18250..19104)
                     /gene="mreC"
                     /locus_tag="IVP39_000796"
     CDS             complement(18250..19104)
                     /gene="mreC"
                     /locus_tag="IVP39_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7585887.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(19259..19897)
                     /locus_tag="IVP39_000797"
     CDS             complement(19259..19897)
                     /locus_tag="IVP39_000797"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7585888.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(19898..20548)
                     /locus_tag="IVP39_000798"
     CDS             complement(19898..20548)
                     /locus_tag="IVP39_000798"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7585889.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(20561..21460)
                     /gene="rsgA"
                     /locus_tag="IVP39_000799"
     CDS             complement(20561..21460)
                     /gene="rsgA"
                     /locus_tag="IVP39_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7585890.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(21625..23694)
                     /gene="pknB"
                     /locus_tag="IVP39_000800"
     CDS             complement(21625..23694)
                     /gene="pknB"
                     /locus_tag="IVP39_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7585891.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(23691..24431)
                     /locus_tag="IVP39_000801"
     CDS             complement(23691..24431)
                     /locus_tag="IVP39_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7585892.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(24444..25802)
                     /gene="rsmB"
                     /locus_tag="IVP39_000802"
     CDS             complement(24444..25802)
                     /gene="rsmB"
                     /locus_tag="IVP39_000802"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7585893.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(25802..26740)
                     /locus_tag="IVP39_000803"
     CDS             complement(25802..26740)
                     /locus_tag="IVP39_000803"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7585894.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(26733..27224)
                     /gene="def"
                     /locus_tag="IVP39_000804"
     CDS             complement(26733..27224)
                     /gene="def"
                     /locus_tag="IVP39_000804"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7585895.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(27239..29647)
                     /gene="priA"
                     /locus_tag="IVP39_000805"
     CDS             complement(27239..29647)
                     /gene="priA"
                     /locus_tag="IVP39_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7585896.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(29900..30205)
                     /locus_tag="IVP39_000806"
     CDS             complement(29900..30205)
                     /locus_tag="IVP39_000806"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7585897.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(30210..30824)
                     /gene="gmk"
                     /locus_tag="IVP39_000807"
     CDS             complement(30210..30824)
                     /gene="gmk"
                     /locus_tag="IVP39_000807"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7585898.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         8555 a         6725 c         5746 g        10130 t
ORIGIN      
        1 atgatgatta cgattggcaa gaagctaagc aatagggaaa cccatatacc gctgcttgat
       61 tcttctttta ctgttgtagc aactttgttt tcttttgcca gttctgttac ttggctcaat
      121 gttgtatcat ttggcaaaat gattgttgtg aaatgggtag tagtcgtatc tgttgaacct
      181 aagatagcta acccaccgct gttagcgacg gtctgctgtt ctttgtattc accagtgatc
      241 ttatatacac catttgcagg ctgcacttca aattctttga tcttgcctgc ttgtagttgt
      301 tctgtgaatt ttgagtactc gatatctgac gatcgctggc cgttattacc aaaaatgaaa
      361 taaacgacca ttaccatcgc taagattacg aggacataat acaaggcatt tttcatcatg
      421 ccgttgttct ttttgttcat agttgccctc cctaagcgtc aatttaattt aacagttttt
      481 tatcaatcct gaccattcta ttgtaccata atttaattga gatacagcat taatttgatt
      541 gataaacact aggtttcaat acaccgatgt acggaaggtt acgatatgct tctgcataat
      601 ccaagccata accgaccaca aattcatttg gtacgttgaa gccaacataa tctgcttcga
      661 tatttactac tcttccttca ggtttgtcaa gtagtgtaac aattttgact gattttgctt
      721 tgcggtattt gaaaagatcc actaaataag caagtgttct tccgctgtca atgatatctt
      781 caacgatcaa cagatcgcga ccttctacat tcgtatctaa gtctttgacg atttttactt
      841 cccctgacga gactgttgca ttcccgtagc ttgaaacatc catgaaatcc aattccaaat
      901 acgtatcgat agaacgaacg atatctgcca tgaatggtac cgctcctttt aagacaccta
      961 ctactagggg attggtgttt tggtaaattt ctgttaactc tttgcctagt tctttgcaac
     1021 gaacttgtat ctcttcttgt gagatcaaaa ttctctcaat atccttttcc aacatcaggt
     1081 tctccttcca attgcttctt tttgatattt gtaaagaagt atgtagtgta ttttatcagt
     1141 ttcttcacga atactcaaat atgaaaaggc aaaaggtatc aagctatata tatttctttt
     1201 ggcatctgta atgatccaag catgctgcct ttgtgaatta ggtactttct gatcgataaa
     1261 atatctagat actttttttg tgagattctc tgttaattta atgcggtctc ctgcttttct
     1321 tttttcgaca tacagttgca catcagcagg aatccaaatg tcatgagaaa atagagacca
     1381 tgaatctgtt gctttgatgt cccctgcatt ttcaggtttg accagaccaa tccattctgt
     1441 ttcattcaaa tatattcctt gttctggaat cagcaacaag cgctggtctt ctgtctggct
     1501 agttgcttgt ttttcttttt tagagagtac aacaaaatga tactctcttt tcagtatcca
     1561 attattgcca atactgagtt gccattggct ttttttctgt tgccactggt ctaaaatcga
     1621 ctccatttgt tgttctttca aaataattcc tgtttttgag aatgctttgt cgaaaaagga
     1681 gtaaaaaaaa tagtatcgtt cagcatctgt catttcttca atcatttctg cagaaaatcg
     1741 aaattgttcc tcccactgtt cgacttcctt ttcgatcaac tgtcccatat atttttgaat
     1801 gacttggtct gcccattgga tctgttgcga aaattgctgg aaatgtctca acgcctgtgt
     1861 attttcttgt ttcaattgag gaactaccaa atgacgaagc cgatttctct ggatatctaa
     1921 caattggttt gtctgatcct caaagtaaac aagctgcgat tccgctgcat aagtatatag
     1981 gtcttctttg ctaaaactca gcaacggacg aatcagtctg cccgtagcaa atggttgtac
     2041 ttctttgatt ccagaaaaag tatttaactg tcccccacga acgattttca ttaacaccgt
     2101 ctcgatttga tcatctccgt gatgagcagt catcaacgtg tcatattgtc cttcttccat
     2161 cacgcgagtg aattcttgat aacgaaagtt acgagcttct gtttctatgg cggatttggc
     2221 tggatcttcc caaatactta catataaagg gagattcttc tcttcacaat atgagcgaag
     2281 ataggcagct tcttctgctg attcttctct taattggtga ttgatgtgga cgatacctaa
     2341 agaaaaaccg atttggcgtt gcacttgtaa aagtaaatgg agcaaaacca tcgaatcaac
     2401 accacctgat accgctgcta aaattctcga tccttgttgc cagaatcctt gttcattccc
     2461 gtgccgaata aatgactgct gtaatagcgg tgcttttttc ccgccttttt caacgttcat
     2521 cctttccaca ccctttcttc ctaaaaaaaa gagagcggga aaacgtctaa aacgacaatt
     2581 aacctgccct ctgttgatac tatgacccaa atagtcgcac tatcattatt tgcggctttt
     2641 tagctgcgac gtccgccacg gccaccgcgc tttccttctg tgtttcgctt caatgaagat
     2701 aagcggtcat cgctatcttt caaaaatgaa ctcatcaatg aatcgaagtc ttctttttga
     2761 gctgaaggtt gactgcgaga aaaagatttt ttctgtgctg gacgatttcg gttgtcacgg
     2821 ggatcttttt gttgatattt tttaggacgg ctttcttctc cagattcagc aggttgttcc
     2881 tgcgctttcc ggatagatag tccgattttt ccatcatcat ttactgaaat aactttaact
     2941 gttacttcat ctcctacact taaaacatca tggatgtctt ttacatagcc atttgacact
     3001 tcgctaatat gaactagacc cgtttttcca gcacctagat caataaacgc accaaaattt
     3061 gtgattcctg atacttttcc ttgcagtttt gctcctacct cgattgacat aaaaaaaatg
     3121 ttcctcctaa ttatttccct tttttatatt cctgcttatt ttgcagttgt agaactagtt
     3181 gattgatctg tttcatttgt cgagctgtct gtatttgatt ctaatatagg ataaactttt
     3241 tcaccgtctt ttgagtaaaa gaatttgctt ctggcaagtt tagctacata ctcatcatct
     3301 ttcagcaatg aaacatcttg gtgcagctga tcaacgtctg cgttgacttc tttcatttca
     3361 gtatttgctt cgactctcaa ttcgttcaat tgatgcatcc tttgcatgtc atggaaaatc
     3421 tgcacaccta ttatcgcgaa aatgaccaaa gcaccaacaa aaattgcagc cagtctgcgt
     3481 cttcgaaaaa tcagttgacg ttgttgttgc tgaaattcag catacttttt ctttgcatat
     3541 tccgtatcta gcgcagcgat tttattgctt tttttcgtca cttggattcg ctccaatctt
     3601 tttgattgtc tttttcatta taccaatatc gtgaaagctt gtctaatcta tttctctagt
     3661 gtttctgtgc gattttcaga aataatttcg tacatttttt gtgcatcttc ttttttggtt
     3721 gattcatgta attccgtaat tttcacttcc aacgttttgt tgccaaactg gactttcaaa
     3781 atatcgccta ctttgacatc actggaagat ttcgccaatt taccgttgat ttgaattctt
     3841 cctttatcag ctacttcttt tgctactgat cgtcttttga tgattcggga aatttttaaa
     3901 aatttatcta atcgcatatc ttattcctca ctttttaatt tttttattta atcgcaggat
     3961 tttcttacca aaaggcaaca ttagccattc tcgtactgta aacagacgaa gacgaatcac
     4021 tgcataaatg aacacagaag ccccgatccc aactcctatc acgctgacaa aaaaagcagt
     4081 ggaacgatga gttacaggac caaatactat attcaataca ccataataca gcagcagtac
     4141 tactgtcata caaagaaggc tgaacatcaa cttccgtcca aaatgccgtt ctttccaaaa
     4201 agcatttagc tcttttgttt caattcgaac aaaataccaa agcgtcacac ctaatcctaa
     4261 aagggtagac aggcttgcac ctgctgttcc taataaaccg gtcagaaaag aagtagtcaa
     4321 tccttttacc aatagtcccc atcctgcgcc ttttaaagaa gggcgaaacg agttcttgct
     4381 ttgagcaata ctttgataag cttggatgac tgcagtgaaa gcaatggaaa aaacaaataa
     4441 tacaagcgct gcatttcccg cataatcttt aaacaacgca taattgatat aagggagcag
     4501 caaggctaat cccaaagacg cagctaatgc taaagcggtc gtcaatctaa gatagatttt
     4561 ggctgtctgt aaaaactgac gatagatccg gttggtcaaa tgacgagtca acgcaggcag
     4621 gaatgtcgca cttaatgcag tggcaatcac taaacctaat tgtactaaag gctgacctcg
     4681 atcatagacc ccttttgcta tcttcgcacc gtattctgac agtccgtaaa cttccaaagc
     4741 attttttaca aagaaagagt cgatcagctg gaaaaagata agcaatccac tataaatcga
     4801 aaccagtccg ccttctacca gaaaacgccg gatcaagggc tttgacggct gtttgaacaa
     4861 tggaaaccgt cggaagctta acgctcctcc atgaatcttt tgttcatagt agtgaaggat
     4921 cccatatgcg cataaaccgc caaaaacagc tccactcatt gctgccgtcc ctgtttggta
     4981 caccgtccat ccaagctttc taaaagcaaa cgcactacag acaatcacaa aaacacgaac
     5041 gaactgctca actacttgag aaacagcagt tggttccatc aagaatcttc cttggaaatt
     5101 ccctcgataa aaagatagac caggcatcag caaaaaggta aaggaaacta cttggatcag
     5161 cggtttcaat tgtatgtctc ccatcatcca agcaatccag ctgctaaatg caaagacaaa
     5221 caaccacaaa aagacacctg tccaaaatac taagggataa agattttgca aagcttgctt
     5281 tcgctcgaaa ggattctttc tttctgcaac gtactttgat atgaactgcg gaagaccgga
     5341 taaagctaaa gtcattgcta tcccataaat tggataaact tgctgataaa cataaaaacc
     5401 ttcgtcgccg actaagtttt ggaggggaat tcggtaaagg gcacttaata cttttgctat
     5461 gaaagaagca atcgtcaata caaatgctcc ttgcatgacg cgccgcattt ctttatgatc
     5521 caaatcattg ctccttccta cgctgacaga tatttttgct cacgcaacgc tttgacaaac
     5581 ttttggattt cctgaagcca tactgcttct tccatttggt tggggatcgt caagcgtatc
     5641 accatctgtt ctttttcaac gcctagactg gcttttagtt ttgtcgctgt cagtgcttcg
     5701 aataactgtt ccacactata gcgttttgtt ccaactttac ttagagtaaa gactatatgc
     5761 gcctcctgtt tccttatcgt ttctaggagc gctcgatcgc catccatttt gatctgccca
     5821 attgtcaaga gatgtgctac ttcttctgga tattctccaa aacggtcgag cagatcatct
     5881 tctagttcat ctagcatttc ttgcgaatca agttcacgaa ttcgtttata gatctcgatt
     5941 ttctgccgtt gatctgccac gtatgtttca ggtagatacg catcgacacc gaggtcgatc
     6001 tcaacagtgg tcttctccac ttgactattc tttccttgtt tacgagaaac agcttcgctt
     6061 agcatttgag aatacatatc gaaaccgact gcatcaataa atccatgctg ctgagcacct
     6121 aagagattac ctgcaccgcg aatggaaaga tcccgcatcg caatcttgaa tccagatccc
     6181 agttctgtaa aatctttgat tgcctgaaga cgtttttcac tgacttcgtt aaggatcttc
     6241 tgctgctcat acataaagta agcatatgcg acacggctgc ttcttcctac ccgccctcgc
     6301 aattggtata acgtggataa tcccatataa tcagcgttct caacgaacaa cgtatttgcg
     6361 ttcgggatat ctactcctgt ttcaatgatg gttgtagtga caagaacatc atattgacct
     6421 tcaataaatt caaaaagcgt attttctaat tgcgcctctg tcatttgccc atgagcataa
     6481 ccgatccgtg cttccggaac gagttcttgg atttcttcta ctttttgttc gatcgtttct
     6541 acccgattat ataaatagaa tacttgtcca cctcgtccca tttctcgatg gattgcttcg
     6601 cgaatagccc caggattttt ttccataaca tacgtttgga tcggataacg attcgcaggc
     6661 ggtgtttcga ttactgaaag atcccgcacg cctaacatcg acatatgcaa agttctcgga
     6721 atcggtgttg cagtcagagt caaaacatcc acttgtgcac gtaactgttt caatcgttcc
     6781 ttatgtttta caccaaaacg ttgttcttcg tcaatgatca gtagacccaa atcgctaaat
     6841 tcaatatctt ttgacaaaat tcggtgggtt cctacaacaa tatcaacttg acctgtacgc
     6901 agttgttcga tggtttcttt ttgttgcttt cttgttctga aacgacttaa tagcccgata
     6961 tttacgggga aaccttcaaa tctttccagc atcgtttcat agtgctgctg tgccaagatc
     7021 gttgttggaa ctaaaaaggc tacttgtttg ctttccttta tcgctttaaa ggccgctcga
     7081 agcgctactt ctgttttccc ataaccaaca tcacctacga gtagtcgatc cattggtttt
     7141 tctttttcca tatcccgttt gatctccgct gcacttctca actggtcatc agtttcagag
     7201 taggggaagg cattttcgaa ttctttttgg taggcgtcat caggtccaaa agcatatcct
     7261 ttttctgact ctcttgcggc atataatttg attagatcgt cagcgatatc ctcgattttt
     7321 gaagagactt ttcgcttggt tttcgtccat tcacttccgc ctagcttatt gatgcgaggc
     7381 gctttggctt cagaggcgac atatttttgg atcagattca gttgagtaac aggaataaac
     7441 agtttgtcgt cgttttgata caaaatagtc atatagtctt ggtgaacgcc atctacttct
     7501 agcgtttcca tcccaatgta tttcccgata ccatgattgg catggacaac gtaatctcct
     7561 gcttttaact cattatagct tttcaatcgc tctgcgtttg ttactgtttg acgtctcgtt
     7621 tgttttttcg tcgttttttg gaaaatttct ttttctgtga tagctactat tttttcttga
     7681 ggaagttcaa aaccagcctg caaagctcct tcaactaatt gtacctgtcc tctgatcaat
     7741 gcattttttt ctgtttctac tactaatatg tcttcatctc gaagcatttg ttctgctttg
     7801 cgtattctgt cctttgtcgg aataaagatc acaacagtct gtttttgctt gcgccaacgg
     7861 tccatttctg ttttcagcaa tggcatttgt ccgaagaact gttgcatcgt ccgatattga
     7921 aaattatgga ttgcttggaa acgaagattt cccatccctt tttggaagag tgagaaaaag
     7981 gttgtggtga acttttcctt ctgcacgatt ttttgtatat ctgctgaaaa tgtctgctca
     8041 gaaaacaccc gcaattcgct gattttttga gtctgccatt ctgccgcttc cctttcgatt
     8101 tcccgatttg tttccatgat tcgctgataa tcatcaacga ctagtaaact atttgaaggg
     8161 aaataatcca agatcgtcac agattcttgg taaagaagat ctgagtagaa ttttgcttct
     8221 tctgtcggga cacctgcttg ccagcttgaa gtcagctgac cgaagtaatc ttgcaaaaac
     8281 tcgcggtctg cttcttctgt ggtcgcagca agccgttttt ctagcatttt ttgcatttgc
     8341 tctattccat gttctagatc ctctggtccg tagacttgtt cactcgttgg ggaaacccag
     8401 atttcttccg ttttttcgat tgagcgttgt gtttctgctt caaaataccg catagaatct
     8461 atctctacat caaatagttc tacacgaaca ggataggttg tcgttaacgg gtaaatatcg
     8521 ataatgcttc ctcgaatact gaattctccc ggttttgcga ccatctcttc tcgtacatag
     8581 cccatcaaag tcaattggcg aggaagattt tccacgtcta tctcatcacc aatatgccaa
     8641 ttcaattgcg cctgtagcca agttttctta ttcggcagtc gtttgtgcaa agatgcgact
     8701 ggcaacaaat agatacctgc ttttttttct tgaagtgcgt taagcgttgc aactctctcg
     8761 cctcgcgctt ctggagaaga aaaggccatt tcagcagaaa gcacttcatc cactggaaat
     8821 aaatggatct gatcttgcgg aatcacatgt tgcagatctt ctgctaactg attcgcataa
     8881 tacaaatttg gcatcagaat aatcatgttt ttatttgttt tcttaaacgc tcgagcaaat
     8941 agaagtgttt tcgctgagcc cgctaatcct gtgattagtt gtctaggcat tttttcttct
     9001 aaattgctga cccattcttt tacttgcgga tcttttccga ctaattgaat aatatccatt
     9061 ccttttcctc cttcctcaat tgaatcgatt catcgcatcc acgaacgaat gcccttctat
     9121 gagatattcg actgctttaa cagattgaca catgctttct tctatcaaag gctgattttc
     9181 tttgctgaaa ggactaagca catgctggac tactgttttt ttcccttctg gcctgccgat
     9241 tcccaccttg attcgatcaa agacattcgt attcaaatgg gaaataatac ttttgatacc
     9301 gttatgcccg ccggcacttc ccttttgacg gagccggatt tttcccactg ccagatccaa
     9361 atcatcgtag attacgacta attcctctgg ataaatccca aaataagtca ttagtgggcc
     9421 gactgcacga ccagattcat tcatgaatgt ttgtggtttg actaaaagga tcttttctcc
     9481 attatgaaaa aattcggcaa cttctgcttc gaacggattt tttttgaaag tagcttgatg
     9541 ctcgcgtgca attttatcca cgaccataaa gccgatattg tgttttgtat attggtattt
     9601 ggtacctggg tttcctaacc cgacgatcat tttcattttt gttttccacc taattctaaa
     9661 aagcagcaca aaaggctgga ctgctaatta gcggtccagt ccatcgagct tttttctttt
     9721 ttatacttgt caatgcatag tataacacaa aacaaggcgt ttcggctaaa agagaatcgt
     9781 taataaatga taaagaaatt aaaaatttct tcaaagttta atgaaataat tgatatataa
     9841 aaatagtgaa ataacgcaca tgtaagcttt tacatataat acaaccttgt tatctgttat
     9901 attttatagc caaatagcgt gacatggggc ttgagaaatg ttagcataag catgggaaaa
     9961 aacaaatcaa tggaaggatt gatactaaat gactgcagct gcagaaaaaa aagatcatca
    10021 aaaagttatt cttgttggag acggcgccgt aggttctagc tatgcctttg ctctagttac
    10081 tcaaaatatc gctcaagaag taggtattgt ggatatcaat actgctaaaa ctgaaggaga
    10141 cgcaattgac ttgtctcacg ctttggcatt tacttcacct aaaaaaatct attctgctac
    10201 ttacgcagac gcacatgatg ctgacttagt tgttattaca gctggcgcac cacaaaaacc
    10261 aggtgaaact cgtttagact tagtaaacaa aaacctaaaa atcaaccgcg atgttgtcac
    10321 acaaatcgtt gaatcaggat ttaacggtat ctttttagtt gccgctaacc cagtagatat
    10381 tttgacttac tcaacttgga aattctcagg tttccctaaa gaacgcgtaa tcggttcagg
    10441 tacctctctt gactcagctc gtttccgtca agcacttgcg gaattagttg acgttgatgc
    10501 acgtaacgtc catgcttaca tcttaggtga acacggagat tcagaattcc cagtttggtc
    10561 acatgcaaat gttgccggct tgcaaatcta tgaatgggta aaaaacaacc cagaaatcga
    10621 tgaagaagct atggttaacc tattctttgg cgtacgtgac gctgcttaca caattatcga
    10681 gaaaaaaggc gctactttct atggtatcgc agtagcatta gcacgtatca ctcgtgccat
    10741 cttagatgat gaaaatgctg ttcttccttt atcagtattc atgaatggcg aatatggatt
    10801 gaacgacatc tacattggtg cacctgctgt tatcaaccgt caaggtatcc aaaaagtaat
    10861 cgaaattcct ttaagtgaca gcgagcaaga ccgtatggct gcttctgcta agcaattaaa
    10921 agaaattcta gatgaagctt ttgctaaact agacgctgaa taatctttga acatgacgga
    10981 aaacatctat tcggtgtttt ccgttttttt attttatcac ccttatggtt tctaaatttt
    11041 ttcataaagt agagataacc ttcgtctttt cctctaaatt atgctatcat gagaccgttc
    11101 caccctaaac ttttacttaa ggagttgtcg atatgacaag atcttcgcat cgaaaaaaat
    11161 cgaaacatgt tgctttgctt tcagttcttg gtgttctctt agtgctgatc agcttttata
    11221 cctatcgcag tacatactac acaaagcatt ttttgccgaa tacagaaata aatggaataa
    11281 atgtcagcaa tctaacagtc gaaaaagcaa atgaaaaatt aaaagaagct tattctgata
    11341 aaaaactggc tattaaagaa gatggaaaag tctggcagga agtaccaaaa tccgagctcg
    11401 gctacaaaga tgatttcact tcggagcttt ctccgatcct aaatgaacaa aacggatgga
    11461 cttggggaat gacttatgtc tcagcggcag aaaaacaaga aatagatcct gtcagtcaaa
    11521 accaccaaaa actagacact accgttcaga cattgacaac taagctgact gatttgaaca
    11581 aagaccggac acctactact gatgcaacga tcgaaaaagc aggtgactct ttcgttatca
    11641 agcctgaagt caatggaaat acgatcgacg tcgatgctgc ggtcaaacaa ttaaaaagtg
    11701 cggtaaactc tggtaaagat acaattgaat tgactgaatt caaagaaaaa ccaaaagtaa
    11761 catctgaaga cagctcactg aaagaacaac tcgcatccat gaatgctatc gcaaatgtaa
    11821 aagctactta cagtataaac ggtgagactt tccagatccc atcaagtgat attatgagtt
    11881 ggctgaccta caatgatgga aaagtcgatt tagatacaga acaagtacgt caatatgtaa
    11941 cagatttagg cacaaagtat aacacaagta cgaatgacac aaaatttaaa agtacaaaaa
    12001 gaggagaagt tacggtccca gttggcactt acagttggac gatccaaaca gacagtgaga
    12061 cagaagcatt gaaaaaagcg attctggcag gccaagattt tactcgttca cccattgtgc
    12121 aaggtggcac aaccgctgat catccattga tcgaagatac gtatatcgaa gttgatctgg
    12181 agaaccagca tatgtggtat tacaaagacg gaaaagtggc ccttgaaaca gatatcgttt
    12241 ccggaaagcc aactacacct acacctgctg gagtcttcta tgtttggaat aaagaagaag
    12301 atgcaacttt aaaaggaaca aatgacgatg gaacacctta cgagagtccg gtaaactact
    12361 ggatgccgat tgactggaca ggtgttggta tccacgattc agactggcaa cctgaatacg
    12421 gtggagactt atggaaaacc cgcggttcac acggctgcat caacacccca ccaagtgtaa
    12481 tgaaggaatt attcgggatg gtcgaaaaag gaacgcctgt attggtcttc taacaaaaat
    12541 aatatataaa aagaaagcac ccgtacgatt ggtacaggtg ctttcttttt attatctaac
    12601 aatgtgctac ttacatttca gcagcagtaa agacttctga aacatcatca tcgtcttcta
    12661 gcttatctac tagacgttcc aattgctctt tttgttcctc atttaaatca atcgtcgttt
    12721 gtggaatcat cgtcaattct gcttgcgcca aagtgaactc tttttccaac tcatctcgta
    12781 cagcagcaaa atcttctggt gcagtataaa tctcgaatac ttcaggagaa gtttgcagat
    12841 cttctgcccc cgcctctaat acatcctcaa acatcgtctc ttcatcaaca gtcaacccat
    12901 cgcgttcaat agcgatatat cctttgcggt caaacaagta actgactgaa cccgtttctc
    12961 ccagtgatcc accgtttcga gtaaaagcta ctcggacatt tgtcgctgtc cgattacggt
    13021 tatctgtcaa tgcatggact aagattccta ctccacccgg accatatcct tcatacgtga
    13081 tttcatcgta attcgtttca tctacggcac tcgtagcctt tttaatcgcc cgttccacat
    13141 tgtcgttagg catatttgca gcttttgctt tgtccacaac aagccgcaaa gctggattca
    13201 tagatggatc aggaccgcct gctttagctg ccatataaat ttcacgagaa agtttctgaa
    13261 agactttccc tcgttttgca tcttgggcat tttttcttcc ttggatatta tgccatttac
    13321 tatgtcctga catgattttt cctcccttgt tcttttgaga atacacttct attctagtac
    13381 tatttcacaa aaaaacaaag ccacagccaa tctataaacg cttcttgaat gccgcaaaaa
    13441 gcacacataa tatcacagca gtcagagcac cgatcgaatc caacatgaca tcttggaaca
    13501 taggtgttcg tccaccggtc atcatctgat ggaattcgtc caaacctgcg taccctgttg
    13561 ctgaaagcca agcaaggatc gctgtcagcc accaaatttt tagcttgggg aaaacaccta
    13621 aaaacaagct gcctcccaaa ataaaatagg taccgaaatg tgcgcccttc cttaaaaaga
    13681 actctaaaaa tttggcgtag cccagatggt cgatactcac catacttcca ccataattaa
    13741 aacgtatacc cgctaatgtg tctttaaatg gctgattagg cagccagctt tctaatcgcg
    13801 aaacttgtga ttgctgtccg taagtttgag aagaactgta aaataagatt ataacgacta
    13861 tcagtgcgat tactaaataa atatttccgt ttttcatttg tttttttgaa gaattcataa
    13921 gtcacctctg tattttatct tagacttttt ttgaagaaac aacaatcatt ttcatagaga
    13981 cttcttccat aaaaaagtgc tatacttaat tttgtattac aatatgaagg gagttttttc
    14041 atgagttatt cattaaattg gaatctagat tctatttttt ccggaggcag tcattctgat
    14101 gctttgaacc aacggatgaa gcaattggaa gaccaaatga acgagtatta tcaccgcgta
    14161 acaaaatggt cacctagttc tgacaatgca gagcagttaa acgctattct tcaattgcaa
    14221 gaaacgatta cgaatggttt tacccaatgc agcagctata ttactgcatt gctttctgcc
    14281 aatgtcaatg attcagatgc aaaggtctta tcaggcaagc tttacgcaat gctgcctcgt
    14341 ctgcaatcag ccgaaactgt attgtcaaag aaatttgcag aaatatctga caatgattgg
    14401 aatcagatgc tttctcaatc ttcgtttgaa acaattgctt ttcgtttaaa cgagattcgt
    14461 cgtgatggct cacaattatt atcagaagct gaagaaaaca ttataaatac gctttcttta
    14521 gatggtctaa atgcttggag cagtcattat gatacgattg tagccagcat ttcgattccc
    14581 ttcgaacaag atggacaagt agtcgaatta tctgctggac aagccttcaa taagatgatg
    14641 ggcgatccag atccaaaagt cagagaaaca cttttcgcag catgggaaga agcctggaaa
    14701 gaaaaggcac ccctcttcac ggatactttg aatcatttag atggttttag actttctgac
    14761 tatgaacttc atggtgtcac agattttcta caaaaaccat tagagtacaa tcggttgaag
    14821 aaagaaacat tgtctgtcat gtgggataca atccagaaaa acaaacaacc ccttgttaat
    14881 tacttgacta gaaaagctaa tcttttcggt aaagaaaaaa tggaatggca agatcaagat
    14941 gcacctatta ttttaggcga tttgaaagaa aaaaccttta gttttgacga agcagcagca
    15001 tttattatcg aaaactttaa caaattcagt ccaaaaatgg ctaaatttgc ccaatctgct
    15061 tttgaaaaga gctggatcga agcagaagat cgtcctggga aacgtcctgg tggttattgt
    15121 actgagttac cagaaacaaa agaatcaagg atctttatga cctacagtaa ttctgttaat
    15181 gaggttgcga cacttgctca tgaactagga catgctttcc atagcagcac gatgtgggat
    15241 ttaccttctt taaatcgcga atatgccatg aatgtcgcag aaacagcaag tacattcgca
    15301 gaactgatcg tagccgatgc tacattaaaa gtcgcaaaaa caaaagaaga aaaaatcaat
    15361 ttgctagata caaaactgca aaatgcttta gctatgttca tgaatatcca ttcccgattc
    15421 atttttgaaa atcgattcta tgaagcacgg caagaaggat tagtctcaga agataagatc
    15481 acagagatga tggttgaggc gcaaaaagaa ggctatcaag gagctttagc tacttaccat
    15541 ccatatttct gggctgcaaa attgcatttc tttattgatg acgtaccgtt ttataacttc
    15601 ccatacacat ttggctacct ctttagtttg ggcatctatg cttacgccaa ccaaaaggga
    15661 gcaaattttg aagaagaata tatccattta ctacgcgata ctgcctcaat gacgacggaa
    15721 gatcttgcga agaaacattt gaatgtcgat cttacccaac cagatttttg gcaagcaggt
    15781 atcgacatgg tgactgaaga tatccaaacg ttcatggaat tgacagaaga atttatctga
    15841 tttctatttt atcacaataa aaaaagagtt agtccagaat gggctaactc tttttgatta
    15901 atcattctta catgctgaca gcaaagtcag gggcaaacca ttgaactgaa ccaactttta
    15961 cgctttcgcc tggttgagga gcatggatat attgtcctcc gcctaaagca atcgctacgt
    16021 ggtaagttcc gccttgtgac ccccagaata acaagtctcc agcttttgct tgagaaactg
    16081 aaatttttgt tccagctgat tcttgaggaa ctgtccaacc accgatgtca cgaccagtta
    16141 cttgcatgta aacatagcgt gtgaaccctg agcagtcaaa gccacttgga tctttaccgc
    16201 cccaaacata tggagtacca atatatttgt aagcttctgc tacgattgct gcaccattca
    16261 cacttccaga aggagctgga gcaggtgtta caggttttgg ttgttcaggt gttggtgttg
    16321 gcgttggagc cggagtacta cttcctgtgc cgtttcctgt atcaacactt tgatctgttg
    16381 atggtgttgt aggcgcaggt gttgttgatt cttctgttgt tgaagattca ggcactgttg
    16441 ttgattcttc tgttgctgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt
    16501 caggcgctgt tgttgattct tctgttgctg aagattcagg cgctgttgat tcttctgttg
    16561 aagattcagg tgttgttgat tcttctgttg ctgaagattg tgtagctgat gatgattctt
    16621 cagtcattga agattgtgta gctgatgatt cttctgttgc tgagctttgt gcagtagatg
    16681 aagcttgagt agcttgtgct tcagcctctg cttgttcgcg tgcttctttt tcagcttttt
    16741 cttgtgcagc ttgttgacga gcttgttctg ctagacgttg ttgttcacga atacgagctt
    16801 gctctgcttc agcttctgct ttttgacggt tcaagtcagc tttcttgtct tcagcagttg
    16861 cttgttcagc agctaatgaa gttttcagta cgttcaaatc agcttgttta gataaaagat
    16921 cgcctttttg agattccaaa gcagcttggt tttcagctaa ttcttttagt ttagcatcgt
    16981 tttcagcttt tttgtcttct acagcttttt tatcttgttt ttgttgttcc atcaagtcgt
    17041 tgtttgcttt taccatcgtt gtcattgctt gaacgcgtcc aatagcatct gctaatgaat
    17101 cagcattcaa tacagcatcg atgtagtttg agcttgtgtt gcttacttgt gcttcgcgtg
    17161 cttgtttttg gattgtatct tcacgttttt caatacgttc ttgtaaatct gcaatgtctt
    17221 ttactaattg tgcagattct tgacgcaatg tatcttgttt tgctaatagg tcttgtgctt
    17281 gtgtgttgat ttcagaaact tggctttcta aggcatcgat ttgtgattgt gcatctgctt
    17341 gttgattttt caagtctgca atcttttgat cttgctgttg tatctgagaa tcaaaatcgt
    17401 ccgccgcggc agcgatcggt gatgctacgg cagtcaaggt cattgaacat accattactg
    17461 ctgatattaa actctttttc actcttcatt cctccgactg gcttaattta ataaatatct
    17521 aaatatattt aagtctttct taacgatata aaaattgtac catagcagtg tgtcaacgat
    17581 ataatagtta tgttacaaaa atgtttcaaa acaactttta aaaatgacaa aactgttcgt
    17641 ttcagaaaat gatcatgaaa aagccgcgtt aagtctagca tttcttaaga taacaacgat
    17701 tctttttcgc tttcgaccta aaaataaaaa aagaaattat tcatcactga ataatttctt
    17761 aaagggaaaa atgaataata caaatataat catgttcaac agcagtgtag gtcctaaaaa
    17821 ccttgtaata aaatacgtgt tatttactac tgctaattta aaaatcaact ggaccaccat
    17881 cgtaaacaat tcataacccg tgacaaagat gatcatactg aaaaattcag taaagatgtt
    17941 gacatggata acgtctttca ttacatacat caaccataca attaaaggga gagctactgc
    18001 atagataccg ataacgccaa tatagtaagc gtcatagata gccccgagaa caatcgtggt
    18061 aatcagtaaa taacgttttt caaacgccat actgcaacat aataatgcca atatcagaaa
    18121 gtgggcattt gacatatagg tgcctttcga ccactcccct agcattcttg ttaaatgacc
    18181 atctatcaac atcaaaaaga acaaaacgat tggcagatag tacttcattg tttctttctt
    18241 caacatggac taatcctccg ctagccgttg gacaatcgtc acaactgaca cgtcatacat
    18301 ttctgcataa ggtttcacat aaacttcccg atccagccca taactatctg gtttcgtttt
    18361 aataaccgta ccgatcggca agttagctgg agaattccct ccaagaccgg atgtctggac
    18421 aacatcccct tctttgatat ccgtatcacc agttaattgg gtcaccacta aggcatggag
    18481 cttttcatca tagtttttaa gcaaaccaaa cgcttcgcca ttagccgaag atacccgtac
    18541 tggaaaatga ttggagcttt cattagagga tgacagtaat tcgattttag acgaagccgt
    18601 attgacctcg atcactcggc caatcaatcc tttttgcgac atgactgcca tatttgcttc
    18661 gataccgtct ttagaaccct tgtctacgat cagcatgtcc tgccaactgt caggcgaacg
    18721 agtaatgaca tttgccgtta ctttttcgta actagtcagc gtctgattca actcaagttc
    18781 ttttttcaac gcttcgattt ctctctcgta attcttgttt tgctgtgaaa cgtcatcata
    18841 gctatcgatt ttttctttta gccgctcatt ttcggcataa gttgaaaaaa gattatggac
    18901 agaatctact ccatttcgta cccaccgtac tggggcagag attgttttat caaccattgc
    18961 aacactgtca ttaacgatgg attggaagaa attgggcttt ccttcattcg ctcgctgcgc
    19021 agcggtcaca cttagtatcg tcaccacgat gataacaata accaaggtaa taataatatt
    19081 tttattagga ttgaacttct tcacaaagac acctcgaatt aattagactc ttgtgttcaa
    19141 ttttatcact aaatgccgtg aaacaaaagg taagttgctc ctcttaagct tttttttagc
    19201 aaataataga ttctgtcatt tttagcctga aagacaaatt tttttatttg tttatggatc
    19261 aatctttgct ttgaataact gcaatcatac catctgaaaa agaaaattct gcggtatctg
    19321 aaagaaattc gttacttgca taagatgtcg gatattctac ttctttattt tccaatacat
    19381 acttgctgcc ttttaatgta agattcgaga cgggagtcaa gcagcaataa gctaaatact
    19441 tcatatctgg tatctgtctt actatatgct ctccaggcaa ataataagac aaataatttt
    19501 gacaatctct gataataagc tgatgggcaa agggtttgaa gcgaggttcc atacctaacc
    19561 agagattggc taaaagatga tccaatcttc cgcccgtagc tccaatcaaa gtgacttccg
    19621 cttgcgggta acgttcaaat gtttttaaga tacctaactg tgtatctgta tcgtcttttt
    19681 ctgcagggga acgggtatac tctttggctt ttgcaaatac ttccttctgc tcttcgttcg
    19741 acaatgaatc aaaatcgccc actgccagat ctactgggta gcctgcttga agcaggtaca
    19801 gcgcaccacg gtctatcccg acataggcat cgtatttgga cagcagagac tgcggccagt
    19861 tttgcggatt acctccagca gctaataaaa ttttcattta gtctaatgcc tcttttaatc
    19921 gttcgatttg tccgactgga ttttcggctc catagatata tgatcctgca acgaagacat
    19981 ccgcccctgc atctttacag atttttgctg tttctggaac gattccgcca tctacttcga
    20041 ttgcataagt ccaattgttc tctctacgta gatcagcgag ttccgtaatt ttttctactg
    20101 tttcttcaat aaagctttgt ccgccgaacc caggattgac agtcatgact agtacttgat
    20161 cagccaaagg caaaacatgt ttgatcatgg agactggtgt tcctggattg ataactactc
    20221 cagctttaac tcctaagctt tttgtcattt gaagtgcccg atgaatatga ggagttgctt
    20281 cgacgtggac agtaatgata tctgctccag cttttgcaaa tgctggaatg tagttttctg
    20341 gatcaacgat catcagatgg acgtctaacg gtaatttagt aacaggacgg attgcctgaa
    20401 caatatttgg ccctaatgtg atatttggga caaattggcc atccattaca tctacgtgga
    20461 tgtaatctgc gcctcctttt tccactaact caatgtctct ttgtaaattg gcaaaatctg
    20521 cactcaaaat ggatggtgct atcttcattc ttgtttcctc ctattttcgt ttaccttctt
    20581 tttttgcata cattggtttt cttttttcga tttcttgtaa gaattgcaaa taattgtcgt
    20641 aacgtgtctg tgcgatacca cctgtttcta ctctatgttt tacttcgcag ccaggttctt
    20701 tagcatgcat gcactctcta aatttgcaat ggacagaagc ttctacaaat tcaggaaatt
    20761 gtctcggcag ttctcctgct tcaattgtca aaaagtcaat cgagctaaat cctggtgtat
    20821 ctgctactaa cccgccatag ataggaatca gttccacatg acgtgttgta tgccgtcctc
    20881 ttccaagtgc atcagaaatc tctcctgttg ctaaattcag ttccggtgat atttgattca
    20941 gtagcgtcga ttttcctgct ccagactgac ccataaagac cgatagccgt tctgggaagt
    21001 aacggatcag ttctgtcgta tcacccgtat gattgggtac aatcaccgga tagcctattt
    21061 tagtataggt ttgcttgatc tcttcgattt tttctgggtc gtctgacaag tcggtctttg
    21121 tcaaaaaaat aatcggttcg atcgactcgt attccaaggt aactagaaag cggtcaagta
    21181 gattataaga aaatgcaggc tctacgatac ttgtgacaat gattccaaga tcaacatttg
    21241 tgactggtgg gcgaactaat tgattttttc tcggcaagat ttccagaaga tagccgtctg
    21301 tttgattttc actctcaaag atcacctgat ctccgacaag cggcgtgatt ttccgattcc
    21361 ggaaatttcc tcttcctctt gtttgatagg tctctccgtt tgcgtataca tagtaaaaac
    21421 cgctgattgc ttttctgatt tgtcctttga taagtgccac ctccgttttt gtttatttta
    21481 ccatacgaag aggaaagtag gtgttattcc atgcaaactt ctcaaagtat atggcttgaa
    21541 aattgatcaa aagagataga aacacaaaaa agagaatgca acaaatcaat gtcgcattcc
    21601 cttctgatat attttattca gcacttattt tgaactttct gaggtgctag ctgatgaact
    21661 tgtggagctg gaagattctt cttttgaaga gttgcttgtg ctactggagc tacttgagct
    21721 gctagaactg tctgaagagt ctttttcaga actagaagac gctggatctg gtcctttaga
    21781 gtaaattacg ttcacaacca ttcctttggt aattgtcgtt cctgcccctg gtactgtacg
    21841 aatcacttta tctttatcca ccgtgtttga atattcggaa gtctcatgcc cgatatattc
    21901 tgcaccgaca ctagctaaat aactttgtgc ttcgcttttt gtatagccgg agatatcatt
    21961 caatttaggc tctgttcctt ggcttacgac aaaattgatg taagtttctt ctgctacaac
    22021 ttcacccgag gctggatcct gactgatgat cgaacctgat ggtacagtgt cgctatagtc
    22081 ataagtagca ctaatattat tgatattgaa tccttgtgta acaagccaat tacgaacagt
    22141 ttcgtagttt tctcctacat gactactcaa agtcactgtt tctggtcctt tgctgactgt
    22201 gaaagtaatc gtgtctgttt taggatcgac ttctgtatct tctgatggag attgcgaaat
    22261 gatcattccg ctttctacct ctgaactgaa ttcctcttct ttttgaatca agtcagcaga
    22321 aaaccctaat tctttcaaac ttgcttcggc atcttcataa cttttaccac tgtaatcatc
    22381 taatttgatt tttttcgtgc cgctactaat atacagagtg acttcgcgtt tttgcttgac
    22441 tgtcgttccc gctgccgggt ctgtcttcac tacattacct tcttcgattt tgtcatctgc
    22501 aatttcttct gtttttgttg ctacttctaa tttcgctttt gcgagtgttt ctctagcaga
    22561 agcttcagaa agatcagaaa catcaggaac aacaacttct cctcgcccgc cagaagcaaa
    22621 gaatacagct gttccgattc ctaaaacagc aagaatagct aataggatca gccaccattt
    22681 tttctgtcct ttctttttag gatcaggcag aacttcttcc tcctctggca aggtttcgtt
    22741 ttctggcttg tcttttggca gaggcataga tttgaatgaa tcaggcattg gtgcatcttc
    22801 cgtaaggggt gtgataactt ttgtttcatt atccatcaca tgaggctgcc atttttcttc
    22861 gtttgcacga gctggtgata atactgtaga aagatcatca gacatttcct cggctgactt
    22921 ataacggtct gcaggttctt tagccgttgc ccgtaaaatc acgttttcca gtgcttgtgg
    22981 cacatttgga tcaagtgctt ttatagatgg aagatcatct tgaaaatgtt ttaaagcaat
    23041 tgttacagca gattcaccat caaacggtac gctacccgtc aacatctcat aaaggatgat
    23101 ccctaaagca taaatatctg actgcttcgt tgccatactt cctctggcct gctcagggga
    23161 cagataatga acagatccca gcatcgtatt cgtctgcgtg atcgatgttt cagataaagc
    23221 gatagcaatc ccgaaatccg tgatttttac aacaccttca ttatcgatca gtacgttttg
    23281 gggtttcaaa tctcggtgga tgatttgatg gttatgagct aaagaaatag cagaaaggat
    23341 ctgctgcata atatttactg ctgtttcata aggaacagga taatgtgttt ggatatatcg
    23401 tttcaagtcc attcctttga cgtattccat caccagatac tgcatgttgt cttcttcacc
    23461 aacatcgtaa acactaacaa tgtttggatg aacgagttct gttgctgcta aagcttctct
    23521 ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca aatcgtaaga ctttaactgc
    23581 tacatcacga tccaagatta aatcgtgcgc caaaaataca tttgccatac caccgctacc
    23641 gatatttcca gtgatttgat aacggccatt taattttttg cccaactcga tcatacactg
    23701 tcctccttat attcgataag aagaacggtg atattatccg ctccgcctgc ttcattggca
    23761 cgatcaatca gctcagttac tttatgagac aatgggcctt cttgattgat gatcgttcct
    23821 atttcttctt cagacagcat gttcgtcaag ccatctgaac ataacagcag tcgatctttt
    23881 agttgccaaa gatgagtaga aacatctact tcgactgttc caggcatgcc gacagaacgt
    23941 gttaaaatat ttttccttgg atggttcact gccatttctt cgctgatttc tcctgatttg
    24001 actagttcat tgactaaaga atgatcctcc gtcaattgca tgattttatc ttcacgaatc
    24061 aaataagctc tgctatcccc aacattggca atcgtgaacc agtcatctga cagagcggcc
    24121 gctacgattg ttgttcccat accgttatat tccggctgtt cttgtccacg ctgataaatc
    24181 agcatatttt cttcttgaat cgattgaatg aaccattggg cgattttttc atcgtcaaca
    24241 gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg ccatctgact ggcaatgtct
    24301 ccggcacgat gaccacccat cccgtctgct aatacagcaa gcttgagacc tgaacggctg
    24361 gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc ttcctacatc tgattgatat
    24421 tcaatctgca ttttttcacc tcattacttt ttgcgcaaac aacaaatgaa aaatccatct
    24481 gtgtaaaact gatgcggata caaagtcaac attttctctt cgattgatga ttgtagcata
    24541 ggattggtca aaacatcaat cctctcaaaa tcagggtgtc tttccaagaa agcagaaaca
    24601 acgtcttggt tttcttcttt taagatcgta caagtgctat aagtcagaat accggaagac
    24661 tttaaagttg gtgcgcagct ttctaaaatt tccaattgga tttttggtaa attcgctaat
    24721 tcatccggct gttttttata acgaatatct ggttttcgcc gaagcagccc aagacctgaa
    24781 caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt cgaactgttt actgacttct
    24841 ctagcatcca tcttttgagc gtagacagca gaagagacac ctagacgtgc tgcattatct
    24901 tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga ccacttctcc accgcatgac
    24961 ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg gtgctgcaca ggcatctaag
    25021 acatgatgtt caggctcgat ttgcatagac ggcgctacaa gcatcgaact ttcatcttgg
    25081 atcgtcatga cgccatcgtg gaacaatgag ctagaagcta aatgcccttt tttcgctact
    25141 ataccgtatg gactgacttg gcttttttca gctgtgatct cttcttcttg caaaacagtg
    25201 atggcctgat ctctggaaag tcggcgtgta tctactcggg cactagcatg gctaggctga
    25261 tatagcgata aaccgagctt ttcagtttct tcgtatccga tttgatccac cagtcgtttc
    25321 gtcatccata gaggcaggct gattttgaca gaaagtcttt cgataggatc agcaatgcct
    25381 tgcagatctg gtgctccttg acgctgataa ttcctcaaga caccatttac aaatttccct
    25441 gtcccaggat ttccttttac ttttgcgatt tcgaccgcct cgtttaaaat agcatgtgca
    25501 gggactttat ctaaaaattc taattggtat acagaaagat aaagaagcgt cttgacccag
    25561 tcatccactt tttttgcttt agcgatcaat ggtgcaacgt aaaattccaa tagtcgctga
    25621 tggctaatcg taccatagat caattctgta aataaaccca catctttttg atttagctcg
    25681 ctttgtttga tcatctcatt caacaaaaga ttcgagtagg cgccaccctt gttcactcgc
    25741 tctaatgtca gtaatgccat atatcgtact gattttttca tttttttagg gatttttttc
    25801 attatttcac ctgctgacca atctgaactt gctgaccgct tccgttaagg aaatcatgta
    25861 cggcctgttt tcctttacca gctggctgta attcattgat ggctaagacg gtctgtttac
    25921 cgcaagcaat ccatagattt tttttgtctt tccggatgat cgtaccaggt gctttgtctg
    25981 tagtttctgc caatacttca accgataata atttccagcg tatgccctca taggtcgtga
    26041 atgcaattgg ccaaggacgc attccgcgta cttggttgtc aatttcttct gcagttttat
    26101 gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga aaaagttgct tctgactcgt
    26161 cttgaggtac tggtttttgt ccatccaaaa tattgggcaa tgtttctagc aatagctttc
    26221 ttcctaaaag gctaagcttt tcaaacatcg tccctacatc atcttgttta gtgattggta
    26281 tgctttcttg cgcatagatt ccgcctgcat ccattttttt gatcatttcc atgatcgtta
    26341 caccagtttc tttctcccca ttcatgatgg cataatgaac aggcgcacct ccccgatatt
    26401 taggaagtaa agaagcatgg acattgattg cccctaattt tggtacttgc aataactttt
    26461 caggcaagaa ttggccaaat gccgcagtga cgatcacatc aggcaccaat tcaatgattt
    26521 cttccatttc tggcgaaccg gagattttct ctggctgcag cacacggata ccgtgcttca
    26581 gagccgcttc ttttaccggt gtcggcgtaa ttactcgttt ccgcccaact ggacggtcgg
    26641 gctgtgttac cactgcctgg atctcgtaac ctgattctat cagcccttca agaatcggca
    26701 cagaaaaagc aggtgtgccc ataaatacta gcttagtcat caagatgttc ctccatatat
    26761 tcttcaagtt cttcttccgg gatctgctgt atcattttct caatgaacaa gataccgtct
    26821 aagtgatcga tttcatgctg gaaggcacgg gctaaatagc caaaagccgt cacttctatc
    26881 tcttctccgt ctcgatcata ataacgcacg gtcacttcat ctgctcgctt gaccgttcca
    26941 tagacatgcg gaatactgag acaaccttct acgtcaaggc tttcaccttt tgcttcgatg
    27001 atttctgggt taatcagttc aaatttctcg ccttcatcta cttcgatcac agcaattctc
    27061 ttgttttgtc ctacttgagg agcagcgatc cctattccgt cattcgcaat cattgtctca
    27121 tataaattgt ctaataaagc gattgtctcg tccgtgatta catcgattgg ctgagcagtt
    27181 ctttttaatt tatcattagg gtggatcaat ataggatagc gcataatttg gccctttctt
    27241 aaataaagtt taatggttcg ttgtcaattg ccacaaaaag accttgtcgc tgttctttct
    27301 ggcttccatt caaaatttca tccaaaacat gaggaagatt cggttcatgt ttatatttga
    27361 tgattagttg ataataatat cggtttttca ctctcaaaat catgctgggc gttggcccta
    27421 atagcaagct ttgagggctt aacacttctt ttagctgatt ggcaatctga aagattttct
    27481 ttgccgctac ttgttcttca ggatggctgg cggttatttt cacagtaaag taataaggag
    27541 gatagcctcc acgatgccgt acatgcattt cctgctgata aaactgctca taattttgtt
    27601 ttttagctaa ttctatggcg taatgttgag gattgaatgt ttggatcacg acttcgcctg
    27661 ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag caattggaat gtatgctcac
    27721 tagaacgaaa atccggtaaa ttcagggagg tatcagcatt caacacccca actaatgtca
    27781 cttctggaaa gtccaaacct ttagcaatca tttgtgtccc taataatata tctgcttctt
    27841 ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt tcttcgagtg gtatctacat
    27901 ccatacgcaa aatcctagct tgcggataaa gttctcggag ttcctcttct actttttgag
    27961 tacctgttcc atagtagcgg attttattgc cgccacaatt aggacagcga ttgggaatag
    28021 gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt atccatatgc agtgtaagag
    28081 agatatcgca attaggacat ggaagaacaa atccacagtc cctacacatc acaaaagacg
    28141 aatatcctcg acgattgagc agtaggacag tttgttcttt tttgtctaat cgattttgta
    28201 ttttttcctg caaattagca gaaaaagtag aggtccgatg attctgaaac tcttcacgca
    28261 tatctacgat ctcaatagct ggaagctgtg cagctgcttt ggctcggtgg tttaactgca
    28321 ataactgata aacgcctttt tgagctcgtg ctcttgattc taaagagggc gtcgcgcttc
    28381 ctagaactat tgggcaatga tgatatttac tgcgccaaat tgctagatct ctcgcatgat
    28441 aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc ttcatcgata atgatcagcc
    28501 cgatattttt cagcggggca aaaatagcag atcgtgcgcc tactactact tgcgcttctt
    28561 cccgctcaat cttgcgccat tcatcatatt tttcaccttg ggaaagtccg ctatgtaaga
    28621 ccgcaactga ttctccgaag cgtcctttga atcgctcgac catttgcgga gtcaatgcga
    28681 tttcaggcac taacataata gccgttttat tttctgacaa aacatcagcg attgcctgaa
    28741 gatatacttc tgttttcccg ctgccagtga ttccctccag caaaaagacc tgatcttttt
    28801 gctgctgacc tgctgtgatg atttgttcga cagcattttt ttgttctgca ttcagttcca
    28861 atgccgtcgt ttggtcaaat actcggtctt tataagggtc gcgataacgt tctgattcta
    28921 taaactccag ccagcccttt tcttttcctt gattcaagac agcagtactg attcctttat
    28981 ttttgaaata agcaattggt tcttcttggc tcagttcaga taaacacaaa agaagttctt
    29041 tttgtttctt ggcattttgc cgtaattttg cccattcttc ttcgatttgc ttttcagtta
    29101 acaaagaacg gaccaaacga atcgttttta ctttatttcg tgtatggact tcatatttga
    29161 tatctactaa ttgctcttgg cgccagcgaa tcaattgaga aaggctgcct tcttcttctg
    29221 cttccttcca ggaaatcact tctcgctgat caaacaatac ctgaacttct ggtgtgtctg
    29281 ccaaaggata gatcaattta tcatattctg ctttcatcac actcggaagc atcgtttgta
    29341 gacaagtgat tttgaatgcg taagtaattt ttttcatata atcagctaat gaaagtaatt
    29401 cttcattgac caccggagca aggtctagca aacgaataac tgccttcaaa gatggattag
    29461 ctgattcttc tgatttttgg atagccataa caaatccttg gacatgacga ttgccattac
    29521 caaaagggac ttctactcgc atcccgactt gcaaagctgt ttccatttca gagggaacca
    29581 aataagtgaa tggctgatcg gtctgcatag taggtacgtc cacaatgact tcagctgtta
    29641 atgtcatggt tcgtttcctc ctttttttgt cttttcttat caagcgacaa aaagttcctt
    29701 gcctatttta ctagagatcg atggatttgt cttatcttct gccaaatcct aacaaaaaat
    29761 taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt cagacacagc cttttctacc
    29821 agacaataag tggcgaatgt ttgaaaagga aacagctggg tttgcttttc tttttagatg
    29881 ttcgctctat ttttctttat tacatttttt gctcttcacg gatacgtgat tccaattcgt
    29941 gttgttcacg ctctttttgt gcttgacgtt cctcttcttc catcttcaaa cgttcacgtt
    30001 ttaattcagg atgtggatca ttgactacat ttcctgcatc gatttcttct aaagcttgac
    30061 cgacgctttt tactgaatca aaagaatcta gagtaggctg tgcacttgca tccaattcat
    30121 gcgcgcgttt acttgctaaa atcactagag aatattttga atttacacga tctaatagtg
    30181 agtcaattga aggttttaac atcataagac tacatctcct ttaacatttt aatatattta
    30241 ccgattacgc gatctactcg aaaatgttca ctggcaataa tgtttttgat tcgttcaact
    30301 gctagcggca cttcatcatt tacaacagca tagtcgtaaa gagccatcat ttcgatttct
    30361 tcttttgcaa ctttcattcg ttcttcgatt acttcatctg catctgttcc tcggcctacg
    30421 atccgtgatt ttaattccgc taaatctggc ggagtcaaaa agatgaatac accatcgggc
    30481 actttttctt tgacttgttt tgctccttgg acttcaatct ccaagaaaac atcttttcct
    30541 tcatccaatg tcttgttgat atatgacaaa ggagttccat aataattccc gacatattct
    30601 gcgtattcca gcatctctcc agcttcgatc attgattcaa actcttcttt gcttcggaag
    30661 aaataatcaa cgccttcgac ctccccttca cgcattttac gggtagtcat tgagatcgaa
    30721 tactgaaaat cattatcctc actctcaaaa attgcttttc gtactgtgcc ttttcctaca
    30781 ccggatggac cagacaacac gattaataat ccacgctctg acatgacgac gtcctttcaa
    30841 ctagtctttt tgtacttatc gtttattttg cacttttttt ctgcaacttt caaggttatc
    30901 tttaattata gctagaaatt ggtaaaaaag ctaccctgta ccaagaggtc gtaaaaaagc
    30961 tgtcctgcat caagtaataa gaacagccaa acataaacag cttctcatgt ttttcgtttg
    31021 gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt gtataagagg tcgtgaagaa
    31081 gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat agtttctcac atttttgcat
    31141 aaatttggct taatac
//
LOCUS       DACCSK010000020        30748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCSK010000020 DACCSK010000000
VERSION     DACCSK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.020"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(279..833)
                     /locus_tag="IVP39_000808"
     CDS             complement(279..833)
                     /locus_tag="IVP39_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7585899.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(959..2888)
                     /locus_tag="IVP39_000809"
                     /pseudo
     CDS             complement(959..2888)
                     /locus_tag="IVP39_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3194..3910)
                     /locus_tag="IVP39_000810"
     CDS             complement(3194..3910)
                     /locus_tag="IVP39_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7585900.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3911..5734)
                     /gene="glpO"
                     /locus_tag="IVP39_000811"
     CDS             complement(3911..5734)
                     /gene="glpO"
                     /locus_tag="IVP39_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7585901.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5748..7244)
                     /gene="glpK"
                     /locus_tag="IVP39_000812"
     CDS             complement(5748..7244)
                     /gene="glpK"
                     /locus_tag="IVP39_000812"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7585902.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7380..8840)
                     /locus_tag="IVP39_000813"
     CDS             complement(7380..8840)
                     /locus_tag="IVP39_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7585903.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8830..10125)
                     /locus_tag="IVP39_000814"
     CDS             complement(8830..10125)
                     /locus_tag="IVP39_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7585904.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10457..11134)
                     /locus_tag="IVP39_000815"
     CDS             complement(10457..11134)
                     /locus_tag="IVP39_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7585905.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11164..12255)
                     /locus_tag="IVP39_000816"
     CDS             complement(11164..12255)
                     /locus_tag="IVP39_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7585906.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12356..12790)
                     /locus_tag="IVP39_000817"
     CDS             complement(12356..12790)
                     /locus_tag="IVP39_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7585907.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13374..14831)
                     /gene="ascB"
                     /locus_tag="IVP39_000818"
     CDS             complement(13374..14831)
                     /gene="ascB"
                     /locus_tag="IVP39_000818"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7585908.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14828..16723)
                     /locus_tag="IVP39_000819"
     CDS             complement(14828..16723)
                     /locus_tag="IVP39_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7585909.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16781..18556)
                     /locus_tag="IVP39_000820"
     CDS             complement(16781..18556)
                     /locus_tag="IVP39_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7585910.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18606..20102)
                     /locus_tag="IVP39_000821"
     CDS             complement(18606..20102)
                     /locus_tag="IVP39_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7585911.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20296..21918)
                     /locus_tag="IVP39_000822"
     CDS             complement(20296..21918)
                     /locus_tag="IVP39_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7585912.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22246..23559)
                     /locus_tag="IVP39_000823"
     CDS             complement(22246..23559)
                     /locus_tag="IVP39_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7585913.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23804..24028)
                     /locus_tag="IVP39_000824"
     CDS             complement(23804..24028)
                     /locus_tag="IVP39_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585914.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24048..24416)
                     /locus_tag="IVP39_000825"
     CDS             complement(24048..24416)
                     /locus_tag="IVP39_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7585915.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24612..25322)
                     /locus_tag="IVP39_000826"
     CDS             complement(24612..25322)
                     /locus_tag="IVP39_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585916.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25340..25837)
                     /locus_tag="IVP39_000827"
     CDS             complement(25340..25837)
                     /locus_tag="IVP39_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7585917.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25874..26326)
                     /locus_tag="IVP39_000828"
     CDS             complement(25874..26326)
                     /locus_tag="IVP39_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7585918.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26461..27066)
                     /locus_tag="IVP39_000829"
     CDS             complement(26461..27066)
                     /locus_tag="IVP39_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7585919.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27516..28136)
                     /locus_tag="IVP39_000830"
     CDS             complement(27516..28136)
                     /locus_tag="IVP39_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7585920.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28138..29007)
                     /locus_tag="IVP39_000831"
     CDS             complement(28138..29007)
                     /locus_tag="IVP39_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7585921.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29030..29437)
                     /locus_tag="IVP39_000832"
     CDS             complement(29030..29437)
                     /locus_tag="IVP39_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7585922.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29456..30745)
                     /locus_tag="IVP39_000833"
     CDS             complement(29456..30745)
                     /locus_tag="IVP39_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7585923.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8720 a         6678 c         5079 g        10271 t
ORIGIN      
        1 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
       61 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
      121 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
      181 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
      241 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
      301 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
      361 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
      421 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
      481 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
      541 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
      601 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
      661 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
      721 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
      781 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
      841 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
      901 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
      961 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
     1021 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
     1081 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
     1141 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
     1201 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
     1261 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
     1321 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
     1381 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
     1441 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
     1501 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
     1561 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
     1621 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
     1681 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
     1741 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
     1801 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
     1861 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
     1921 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
     1981 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
     2041 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
     2101 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
     2161 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
     2221 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
     2281 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
     2341 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
     2401 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
     2461 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
     2521 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
     2581 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
     2641 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
     2701 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
     2761 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
     2821 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
     2881 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
     2941 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
     3001 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
     3061 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
     3121 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
     3181 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
     3241 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
     3301 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
     3361 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
     3421 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
     3481 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
     3541 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
     3601 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
     3661 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
     3721 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
     3781 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
     3841 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
     3901 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
     3961 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
     4021 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
     4081 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
     4141 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
     4201 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
     4261 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
     4321 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
     4381 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
     4441 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
     4501 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
     4561 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
     4621 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
     4681 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
     4741 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
     4801 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
     4861 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
     4921 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
     4981 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
     5041 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
     5101 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
     5161 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
     5221 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
     5281 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
     5341 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
     5401 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
     5461 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
     5521 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
     5581 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
     5641 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
     5701 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
     5761 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
     5821 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
     5881 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
     5941 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
     6001 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
     6061 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
     6121 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
     6181 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
     6241 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
     6301 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
     6361 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
     6421 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
     6481 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
     6541 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
     6601 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
     6661 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
     6721 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
     6781 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
     6841 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
     6901 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
     6961 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
     7021 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
     7081 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
     7141 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
     7201 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
     7261 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
     7321 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
     7381 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
     7441 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
     7501 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
     7561 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
     7621 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
     7681 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
     7741 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
     7801 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
     7861 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
     7921 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
     7981 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
     8041 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
     8101 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
     8161 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
     8221 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
     8281 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
     8341 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
     8401 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
     8461 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
     8521 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
     8581 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
     8641 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
     8701 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
     8761 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
     8821 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
     8881 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
     8941 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
     9001 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
     9061 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
     9121 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
     9181 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
     9241 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
     9301 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
     9361 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
     9421 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
     9481 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
     9541 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
     9601 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
     9661 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
     9721 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
     9781 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
     9841 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
     9901 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
     9961 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    10021 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    10081 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    10141 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    10201 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    10261 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    10321 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    10381 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    10441 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    10501 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    10561 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    10621 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    10681 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    10741 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    10801 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    10861 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    10921 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    10981 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    11041 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    11101 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    11161 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    11221 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    11281 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    11341 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    11401 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    11461 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    11521 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    11581 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    11641 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    11701 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    11761 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    11821 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    11881 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    11941 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    12001 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    12061 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    12121 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    12181 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    12241 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    12301 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    12361 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    12421 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    12481 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    12541 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    12601 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    12661 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    12721 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    12781 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    12841 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    12901 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    12961 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    13021 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    13081 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    13141 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    13201 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    13261 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    13321 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    13381 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    13441 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    13501 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    13561 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    13621 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    13681 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    13741 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    13801 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    13861 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    13921 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    13981 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    14041 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    14101 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    14161 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    14221 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    14281 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    14341 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    14401 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    14461 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    14521 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    14581 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    14641 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    14701 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    14761 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    14821 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    14881 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    14941 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    15001 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    15061 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    15121 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    15181 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    15241 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    15301 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    15361 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    15421 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    15481 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    15541 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    15601 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    15661 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    15721 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    15781 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    15841 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    15901 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    15961 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    16021 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    16081 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    16141 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    16201 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    16261 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    16321 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    16381 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    16441 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    16501 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    16561 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    16621 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    16681 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    16741 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    16801 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    16861 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    16921 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    16981 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    17041 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    17101 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    17161 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    17221 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    17281 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    17341 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    17401 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    17461 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    17521 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    17581 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    17641 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    17701 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    17761 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    17821 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    17881 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    17941 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    18001 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    18061 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    18121 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    18181 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    18241 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    18301 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    18361 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    18421 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    18481 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    18541 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    18601 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    18661 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    18721 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    18781 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    18841 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    18901 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    18961 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    19021 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    19081 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    19141 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    19201 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    19261 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    19321 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    19381 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    19441 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    19501 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    19561 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    19621 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    19681 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    19741 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    19801 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    19861 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    19921 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    19981 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    20041 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    20101 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    20161 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    20221 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    20281 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    20341 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    20401 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    20461 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    20521 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    20581 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    20641 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    20701 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    20761 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    20821 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    20881 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    20941 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    21001 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    21061 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    21121 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    21181 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    21241 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    21301 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    21361 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    21421 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccatgcaga
    21481 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    21541 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    21601 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    21661 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    21721 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    21781 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    21841 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    21901 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    21961 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    22021 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    22081 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    22141 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    22201 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    22261 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    22321 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    22381 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    22441 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    22501 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    22561 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    22621 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    22681 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    22741 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    22801 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    22861 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    22921 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    22981 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    23041 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    23101 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    23161 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    23221 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    23281 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    23341 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    23401 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    23461 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    23521 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    23581 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    23641 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    23701 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    23761 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    23821 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    23881 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    23941 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    24001 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    24061 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    24121 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    24181 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    24241 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    24301 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    24361 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    24421 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    24481 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    24541 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    24601 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    24661 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    24721 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    24781 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    24841 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    24901 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    24961 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    25021 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    25081 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    25141 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    25201 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    25261 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    25321 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    25381 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    25441 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    25501 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    25561 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    25621 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    25681 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    25741 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    25801 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    25861 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    25921 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    25981 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    26041 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    26101 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    26161 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    26221 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    26281 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    26341 attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    26401 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    26461 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    26521 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    26581 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    26641 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    26701 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    26761 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    26821 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    26881 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    26941 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    27001 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    27061 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    27121 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    27181 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    27241 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    27301 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    27361 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    27421 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    27481 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    27541 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    27601 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    27661 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    27721 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    27781 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    27841 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    27901 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    27961 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    28021 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    28081 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    28141 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    28201 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    28261 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    28321 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    28381 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    28441 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    28501 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    28561 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    28621 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    28681 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    28741 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    28801 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    28861 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    28921 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    28981 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    29041 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    29101 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    29161 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    29221 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    29281 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    29341 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    29401 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    29461 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    29521 catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact agcaaagcgg
    29581 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    29641 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    29701 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    29761 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    29821 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    29881 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    29941 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    30001 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    30061 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    30121 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    30181 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    30241 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    30301 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    30361 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    30421 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    30481 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    30541 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    30601 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    30661 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    30721 cgatgataaa attgataacg cccatgtt
//
LOCUS       DACCSK010000021        30005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCSK010000021 DACCSK010000000
VERSION     DACCSK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.021"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(75..1216)
                     /locus_tag="IVP39_000834"
                     /pseudo
     CDS             complement(75..1216)
                     /locus_tag="IVP39_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1364..2917
                     /locus_tag="IVP39_000835"
                     /pseudo
     CDS             1364..2917
                     /locus_tag="IVP39_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            complement(3025..4107)
                     /gene="uxuA"
                     /locus_tag="IVP39_000836"
     CDS             complement(3025..4107)
                     /gene="uxuA"
                     /locus_tag="IVP39_000836"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7585924.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(4098..5744)
                     /locus_tag="IVP39_000837"
     CDS             complement(4098..5744)
                     /locus_tag="IVP39_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7585925.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5928..6590
                     /locus_tag="IVP39_000838"
     CDS             5928..6590
                     /locus_tag="IVP39_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7585926.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            6734..7297
                     /gene="ahpC"
                     /locus_tag="IVP39_000839"
     CDS             6734..7297
                     /gene="ahpC"
                     /locus_tag="IVP39_000839"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7585927.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            7307..8836
                     /gene="ahpF"
                     /locus_tag="IVP39_000840"
     CDS             7307..8836
                     /gene="ahpF"
                     /locus_tag="IVP39_000840"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7585928.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            8993..9343
                     /locus_tag="IVP39_000841"
     CDS             8993..9343
                     /locus_tag="IVP39_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7585929.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            9442..9972
                     /locus_tag="IVP39_000842"
     CDS             9442..9972
                     /locus_tag="IVP39_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585930.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            10126..11277
                     /locus_tag="IVP39_000843"
     CDS             10126..11277
                     /locus_tag="IVP39_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7585931.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(11386..13425)
                     /locus_tag="IVP39_000844"
     CDS             complement(11386..13425)
                     /locus_tag="IVP39_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7585932.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13483..14238)
                     /locus_tag="IVP39_000845"
     CDS             complement(13483..14238)
                     /locus_tag="IVP39_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7585933.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            complement(14330..15460)
                     /locus_tag="IVP39_000846"
     CDS             complement(14330..15460)
                     /locus_tag="IVP39_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7585934.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(16001..16207)
                     /locus_tag="IVP39_000847"
     CDS             complement(16001..16207)
                     /locus_tag="IVP39_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585935.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(16223..16837)
                     /locus_tag="IVP39_000848"
     CDS             complement(16223..16837)
                     /locus_tag="IVP39_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7585936.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(16984..17667)
                     /locus_tag="IVP39_000849"
     CDS             complement(16984..17667)
                     /locus_tag="IVP39_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7585937.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(17776..18234)
                     /locus_tag="IVP39_000850"
     CDS             complement(17776..18234)
                     /locus_tag="IVP39_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7585938.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(18318..20081)
                     /locus_tag="IVP39_000851"
     CDS             complement(18318..20081)
                     /locus_tag="IVP39_000851"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7585939.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(20302..21762)
                     /locus_tag="IVP39_000852"
     CDS             complement(20302..21762)
                     /locus_tag="IVP39_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585940.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(21970..22896)
                     /gene="glsA"
                     /locus_tag="IVP39_000853"
     CDS             complement(21970..22896)
                     /gene="glsA"
                     /locus_tag="IVP39_000853"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7585941.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(22919..24346)
                     /locus_tag="IVP39_000854"
     CDS             complement(22919..24346)
                     /locus_tag="IVP39_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7585942.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(24377..25591)
                     /locus_tag="IVP39_000855"
     CDS             complement(24377..25591)
                     /locus_tag="IVP39_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7585943.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(25932..26174)
                     /locus_tag="IVP39_000856"
     CDS             complement(25932..26174)
                     /locus_tag="IVP39_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7585944.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(26206..26763)
                     /locus_tag="IVP39_000857"
     CDS             complement(26206..26763)
                     /locus_tag="IVP39_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7585945.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(26776..26964)
                     /locus_tag="IVP39_000858"
     CDS             complement(26776..26964)
                     /locus_tag="IVP39_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7585946.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(26977..27543)
                     /gene="amaP"
                     /locus_tag="IVP39_000859"
     CDS             complement(26977..27543)
                     /gene="amaP"
                     /locus_tag="IVP39_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7585947.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            28773..29246
                     /locus_tag="IVP39_000860"
     CDS             28773..29246
                     /locus_tag="IVP39_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7585948.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            29255..29482
                     /locus_tag="IVP39_000861"
     CDS             29255..29482
                     /locus_tag="IVP39_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7585949.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         8880 a         5768 c         5170 g        10187 t
ORIGIN      
        1 atctggtatt ttggggtcat tttctggata tttcttttta tttgaaaaat gcgttcacag
       61 acattattca aatgttagag agatctgttt tgtttaaatc tttttgcaga aagcattgtt
      121 gctatactta atagaatgat tccgctccac agtataactt tgttttgtga ctctccggat
      181 tttggtagct gtttattttt cgtttcagga cagcaaaggt tgtatccttg ctacttttta
      241 aggtttcagc tgattgggcg ctatcaaata ttcgtgattt tttattcagc tgctcttcat
      301 ttatttttag aattgctaga tttgattttt tactctcttt ttttccatcc tcttgaattg
      361 tttttttgtt tattttttct ggtattcctt ggtctcttgc gtccgtatct ggttctggag
      421 tcacatctgg tttttcttct ggatctacct ctggtttttc tggattttca gtttcattat
      481 ctgtgtctgg atttgtttcc ggtactgttt ggtttccaga atccgtatct ggttctggag
      541 ttacatctgg tttttctcca ggggttattt caggttttga tttattgtct ggctctggtt
      601 gagtttctgt atcgggttct tctccaggtt ctatttccgg tttctgttct ccgtctggat
      661 tttctgtagg cggtacttct ggcttatttt cattttctgg tttatctgtt ggttcttcaa
      721 caagatctcc tcctgagtcg atggctgaat cttgctcaat tgtctgccgc tgactatacg
      781 gcgttaaatg atgatcgttc ggtagtccat ctgatacctt gacagtaaaa agcgctggtt
      841 ttaacaagtc ctgatttaat gtaacggtga aggtaaatat cgcttgcttg tgttggatag
      901 tcaaagtgtt ctcgtcatag ccaatcgaca aagtgggatc aacattctca aatgtaacag
      961 ctgattgtag tataggtgct aagtctcccg aatagtcaat ttttgctttg atggtcgctg
     1021 tttgtccttt tcttaatttt gcgactcctg aattgaagga tgaaaattca tcctgaaagg
     1081 taattcgatt gacgttggcc cacttggttt ctgtccattt ggctgtttct gtctcctctg
     1141 tgtggactgt ctgatttata gtaagcgata agcttaaggc tgcaaatgtt gatattaaga
     1201 tggttgaata tctcataact ctctcactcc ttgatttttt tgtatgcaag aaataagata
     1261 gtagagattc tacctatatg tttactgttt ttttgtctaa atttatgaaa aaaacagtaa
     1321 tattttcaag ttttaacaat ttaagtaaaa aggagcctct ttcatgcaaa ctcaagaagc
     1381 gattaaaacg tttatcttga agaaaaaaag caatacaaaa ttagatattt ttcttttcct
     1441 atctgaacat cgtttcttta ttactacaca atatttagca gatcatttcc atatgtctga
     1501 atcaaatttt ttactataca tcaaagaatt agaacaagac tttgagcgtc tcaatctaac
     1561 agaactgcat attgataagc aaaaaccttt tttaaagttg aattttgaag gaattgatcc
     1621 ggcttactgc tattatcgtc tatttggaag atactgcaac gaatcagtca gttatcagat
     1681 cttgacttct ttgttcagct gtcagacaaa ttcgattatc agtttcagtc agcaaacaaa
     1741 ctatagtgcc tcttatttgt atacgaaaat gaaaaagatt aatgcatttt tagcatagta
     1801 cggtttgtct atctcctttt ctaataatgg cagaaagtct ttttctggaa aagaggtaca
     1861 aattcaatat gcagcattag atatttattg gaacatcttt tcaagtactg ctacagaatt
     1921 ttatgatgaa gatccgcaag tagtggcttt tatgatgaat acttttttta aaaaagaagt
     1981 acttgaccga ctaaatagcg gtatgataga taaattatat tttctattaa aaatatgtaa
     2041 agagaatttt cctgcaactt ctgtgaaaga cgtacaaaaa gaattcacag agtatgaata
     2101 tattgacttt tttattgatc tctctgtaga tattttgcga cctaatctgc caatttgtcg
     2161 cgaacaacgc atcttagtga atattttagc tcgactttct atcacgaaaa tcgaatcgga
     2221 agaaacaagc ttggagcaat acaaactttt aaaaaaagca aacgttcctc atgttcttta
     2281 ttcaagagaa atggtggagt ccctttgtac tgtatttgat ttgtttatac ctgaaaaaga
     2341 aaagattctt tactcattgg cattcgcacg aaataaactt tataatggat ttcttaactt
     2401 caatcaacca aatacgcccc ttcctacttt catgctttat caagaacata gcgtattgaa
     2461 agaagtccaa accgccattg aacaatttta tatggatttc agagaacaaa atcaacactt
     2521 gttaccagtt attttagaaa aggaaaatgt gcaatggatg gttgaagacc tagtacattt
     2581 gtatgatcgc tacaaaaaac agccgcgaat tgtgattggt gtgaactata cgcgtgattt
     2641 ttatatcagt aaagatctga tcgttaagat agaacaaatt ttttcaaatg aatcgatcaa
     2701 aatacaaaat gattttatgg agaactgcaa catcgttatc agcgactgcc ctttgaaaca
     2761 attgccttct catatcaaaa aaatctatat tttaaaagga atcatcacac cagaagactg
     2821 gaaaaaagtg attatgagga tctcagaata tatctttgaa ttaaaagaag aagcactcat
     2881 ggatcaatcg aaaaaagaaa atcaagttac ttcctagacc aaataagata cataaatccg
     2941 aatgcttatt aacaacctcc ggatttatgt atcaagaatt tgaacatcct tcttatttac
     3001 ataataaaat tacagttttt ttcactaatt atctttactt ttgtttcctt tttcgattgc
     3061 ttcccataat ccatttagat aaacagcacc taatgctcga tcatataagc cgtatcctgg
     3121 tcttcctgtc tcattccaga tcattcttcc atgatcagga cggatgtatc cgttaaatcc
     3181 gttgtcatac attgttttta gtatgcggta catgtctaat gaaccgtatt cggatttatg
     3241 tggtgcctca tagaaatctc gattagctaa aaatttaata tttcgaacat gtgcaaatgg
     3301 gattcgatct ctttggcaaa attctgctaa aatcccatat acatcatttt ctggatcttc
     3361 tgcaatactc cctgtacaaa gagtgattcc gttataaggg ctgtctacta tgcgacacaa
     3421 ccaatctaaa tcttctcgat tctttacgat tcgagctaat ccaaaaatcg agtatggcgg
     3481 atcatctgga tgtacagcca ttttgacccc tacctcttca catgttggga tgattgattg
     3541 gagaaaatag gcaaaatttt ctcgaagttt ttcttcgtct actgatgcat aggcttcaag
     3601 caaggatttg ataggagcta agcgctcagg ttcccatccc ggcaatccaa attcattgct
     3661 ggaatgttcg atgcgcgcga cgatttctct tggatcatct ggaatatcat cagatataaa
     3721 agctaacgtg gatgagccgt cttctaagcg gtagtcaagg tcagatttca cccagtcaaa
     3781 tacaggcata aaattatagc agatgacttc tattccatat tctgccaagt ttcggattgt
     3841 ctgcttatag ttttcgatat actgctcacg ggaaggtaat ccaattttaa tggcatcatg
     3901 cacattgacg ctttcaatta cttttagtgt taacccacga gactcaattt cttcttttag
     3961 ttctttgatc ctctccctag gccatacctc tcctactggt atatccatca atgtgcctac
     4021 aatccctttg accgcaggaa tctgtctgat ttcatctaag gtaatctttt cttcctgtga
     4081 tccgtaccat ctaaaagtca tttccatgat aaatttcctc catgttcttg taagtaatcc
     4141 tgtaatgttt ttcgtacggc atttggtcct gctaccatct tgatgaacat ttcttctatt
     4201 tttctgccaa gctctgcttg gtaaagatct acaccaaaaa tttctttatt tgacaaaata
     4261 ggagccattt tctcatgaat atgtgatgta tcttgcgtac ccagttgaat atcggaaagc
     4321 ttatgctgaa ggtctgtcaa tagtgggtct ggactcggga tgaatggttt tccttgatca
     4381 tcaatgccaa taaggtatcg aagccaagca gcgagagtca atggtatgaa tacaagtgtc
     4441 tgcgggtctt taacttctat ttccatatat tttttaatcg tctcaccata acgaatcgcc
     4501 accttttgag aggtatccgt ggcgattcgc tgaggcatat taggaatatt tggattagaa
     4561 aagcgcacag taattacttc ttccagaaag atctttggat caatgatacc tggagactcc
     4621 acaactggga ggccttcatt gtacccgatt tcagaaacta gcagcgataa atctggatct
     4681 ttcatctcct cagctatgct cctatacccc agtaaacaac ccataatagc taaagacgta
     4741 tgcaaaggat taaggcaagt cgtcaccttc atcgtatctg ctttatctac cgttgcacga
     4801 tcagtcaaga ataccccagc tttttcgaat ttcggacgtc cattgggaaa atgatcttcc
     4861 atgattaaat aatggatgtc ctctgtattg acaaatggag caatattatt tccttttgcg
     4921 gtttggaaaa tcgacatatt tgaaaagccc tcttttgcca acattttgct aactgacaag
     4981 gaaggattcg gggtgatacg atcaatcatc gtccatggaa acgcaacaat ttcttcattc
     5041 gataagtatt ggataaatgt attttgtaca taaccttcct tttgccattc ttttgcgatc
     5101 gttagcaaag aagtacgtaa ttgactaccg ttttgtgaaa aattatctgt agagacaagt
     5161 gcaatcggcc aagctccaga taaaaaacgt ttataaagta gcacagcaag aatactcatt
     5221 gtatgtgatg gcttctttgg accatttttt atatcgactg caatcggtcc tatatattta
     5281 ttatttgtat ctttcaatcg ataacctttt tcagtaatag caaaagtaac taattgtaaa
     5341 ctaggatttt caaagtgtcg aatcatctta tcaaagtgtt cagaacaatc tggatgacaa
     5401 tacagactgt cagctgtcgc agctaatata cgtttgacga actctccact gcttggcatg
     5461 accacttcaa gaatcaaatc atcaaaatct tggtaagccc tttcaattat ttcttcatcg
     5521 aatgtttcac aaacaatgac accagtggac aattcgccta gatccgctaa acgttgagcg
     5581 atttctgcat gaaatcctcg ataaagattt ccgccaccga aatggatcca gacaggattt
     5641 ttttgtccat tttctcttaa tgatgcacgg tcaaaatgag gtatttctac ttctttttga
     5701 gataattcag ggataaagtt cctccagttg tttaaatcaa tcatttttcc cctcctaaac
     5761 taacatatta gtttttgtat tcaagtgaat tttatgcaat aaaaatcgct atgtcaacca
     5821 aaattttact atttatacaa atttactagt taatgtgaca taccagattg tttttcagac
     5881 ctcttctgtt atactacgtt catagaaatg attttggagg aataaagatg aaaagcagct
     5941 tgcaatcaca agcttatcaa gcgattcgta aacaaattat ctatgctgac ttagaacctg
     6001 gaaggaaaat atctgaaaag gcactggagg aacatcttgc tatcggccgg actcccattc
     6061 gcgaagcatt gattcaacta cgtcagcaag aactcgtgta tacgatacct caaagtggta
     6121 cgtatgtttc caaaattaat cttgtttctg cagaaaatgc tcgctttgtc cgagaacagt
     6181 tggaagagca aatcatgatt gaatgttgtt ccaaattgga cacacaaaca cgaatagtgc
     6241 tagaaacgat tttagaagaa caagaaaaag cagtcaaaat aaatgacgaa cgcgcttttt
     6301 tccgtgcaga taatttattt catgaagctt gtttcgaaat tgcaggaaga aaagaagttt
     6361 ggaactggtt agatgaccat aatacccatt tagaacgttt ccgctggtta cgagtgacca
     6421 ttcaggggct tccatgggaa acgatcatgg aacaacatta tgatttactc aaggctttag
     6481 ttgataaaaa accagaagaa gctcgatttc aaacgggatt gcacttacac atgatgctga
     6541 aagaacagga aactgttact caacgttatc ccgagtattt ccagcagtag cattttcttg
     6601 cgacctcttc tatttttata attaaccttc gtttttgagt aatctttatt aaataacaaa
     6661 atatccattg actttgcaac tccaactaaa ttacaataat tataaataaa taattattat
     6721 tggaggtttt actatgtcat tgattgggaa aaaaatcgaa gaatttaatg cacatgctta
     6781 tcatcaagga gaatttttag aagtcagtga aaaagatctt atggggaact ggagtattct
     6841 ttgcttctat ccagctgatt ttacttttgt ttgtcctact gaattagaag acttacaaga
     6901 acaatacagt acattgaaat ctttaggtgt tgaagtcttt tcttgctcta ccgataccca
     6961 ctttactcac aaagcatggc atgatacatc agatgctatc ggtaaaatcg aatacgtgat
     7021 gatcggtgat ccgtctcatc agatttctcg tatttttgat gttttggatg aagagcaagg
     7081 attagctcag cgaggaacat ttattattga tccagacgga atcgttcaag ctatggaaat
     7141 caatgcagat ggaatcggtc gtgacgctag tgcactgatt gataagattc gcgctgctca
     7201 atatgtacgt acccacccag gtgaagtttg tccggcaaaa tggaaagaaa gtggcgaaac
     7261 attgaaacca agttttgatc ttgttggaaa gatttaggtg gtggatatgt tagacaaaga
     7321 aactaaacaa aatctcgaac aatatttagc actgattgaa tctcctattg ttttttctgt
     7381 tagtttggat aatagtgaaa atagtcaaaa actagctgaa tttacgaagg aaatagcaga
     7441 aatgtctcct aaaatctctt gggaaaaaag agattcgatg cgcacaccta gtttttctat
     7501 caatccagtt ggcaaagaaa gtggaatcgt ttttgctggt atccccctcg gtcatgaatt
     7561 ttcctctttt ttactggcta tgttacaaat tagtggacgg gcaccgaaaa tcagtgaatc
     7621 tttgagtctt aaaatcaaaa aaataacaca aacattgcat tttgacagtt atgttagctt
     7681 gacttgtcat aattgccccg acgttgtaca ggcattgaat atattagctg tattaaatcc
     7741 aaatattact catacgatga ttgaaggtgg gatgttccaa aaagaaatcg atgataaaaa
     7801 aatcatggca gtgccaactg tgtttttaaa tggagaagaa tttagtagcg gacgcatgac
     7861 gatcgaacaa attgttgaaa aaatcactgg ccctttgatt gaagaagagt tgtttgaaaa
     7921 agaaccttat gatgtattag tgattggcgg tggaccagca gctagtagtg cagctatcta
     7981 tgcagcacgt aaaggaatcc gtacaggact tgtcgctgat tctttcggcg gacaagtcgt
     8041 tgaaacacta gggattgaaa atatgattgg tacctcttac acagaaggtc caaaattgat
     8101 gcagcaagta gaaagtcatg tgcgtagtta ccctatcgac attatgatga atcaacaggc
     8161 cgtttcctta aataaagagc aacattttat taatattggc ttagctaacg gtatatcact
     8221 taaagcaaaa accgccatca tcgcgacagg tgcgcactgg cgttctatca atgtaccagg
     8281 cgaaaaagag tttaaaaata aaggaattgc ttattgccca cactgtgacg gccctttatt
     8341 taaaggaaaa gagattgctg ttatcggtgg tggtaattct ggtgttgaag cagcaatcga
     8401 tttggctggg ttagcaaaac atgtatatgt tctagaattc ttaccagaat taaaagcaga
     8461 tcaaatctta caggataaac tagtctctct tgcgaatgtc actgttatca aacatgcagc
     8521 gacgaaagaa atcagcggta cgaatcaggt agaatctctc tcctatatcg atcgccgtac
     8581 gatggagatg catacactag ctgtttcagg agtattcatt ctagtcggct tactccctaa
     8641 tacggattgg ctagatggaa cgatcgaaat gaatccaaga aatgaaatag tgaccgagaa
     8701 aaatggcgca acgaatatcc ccggcgtttt cgcagctggt gattgtacag atagtccata
     8761 caaacaaatc attgtttcaa tgggttcagg agctactgca gctttaggag cattcgatta
     8821 tctgatgcgc acttgagcta tgaagattgt aaaaagtcag agagcggaaa aaaggattgt
     8881 gacaagaatt ttgtcacaat cctttttgtt tttttgaata atcgcttttt cttctctttt
     8941 tcttatatac tataagtagt aaataatttg aaaaaaagaa aggtgactga ttatggcgca
     9001 aaatcgtatt tttacgacaa gttttgcaag cgtttatcct atgtatgtag caaaagcgga
     9061 gagaaaagat cgtataaaag aggaagtaga tgaaatcatt tgttggctga cgggttattc
     9121 ccaaacagaa ctagaatatc aactacaaaa acaagtgact ttcgaacaat tttttgcgga
     9181 agcccctgtc cccaatcctt cacgttcctt gattacaggt gttgtttgtg gtgtacgagt
     9241 agaagacatc gaggataact tgatgaaaga aattcgttat ttagataaaa tgatcgatga
     9301 gctagcaaaa ggcaaaaaaa tggaaaagat cctcagaagt tgatttttcc agagatattc
     9361 ttctccttcg ctcattttgt aacatttaat ttggtaaaat gactagttca cgttaaaata
     9421 agtcaaagga ggttttctta tatgtttgag acgattaaac aaaatattca aaaatacgcc
     9481 ttattaagag ctgctgttta tattatttct gggattgcga ttgtgctaaa cccttctgct
     9541 gtttttcatt ttatcggtta tctgatcacc gcctatcttg ttttgcttgg gttgttgaat
     9601 ctttttgagg cttaccgttt taaaaggaaa acaggttctt ggagtatcgg tctgttcagt
     9661 ggtctttttt tcctgatctt agctctgatc gtctatgctt ttgcacctgc aatcgtttct
     9721 atcctgccga ttcttcttgg attagctgtt atcatcaaca gcttgttcca gttgttcttc
     9781 agcatgaata caaaagctaa aagttggagt gtttatagta gtattctgtt gttgattggc
     9841 ggattcgtct tattgttcaa tccattcaaa agtttgatgc ttctattcca aatattcggc
     9901 tttattttga ttttcatggg catccaggag attatcaatt tcatcagaat aagaaaaatg
     9961 actatacgtt aatcattttt agttattaaa atagaccgta taaaaaccaa caatccaaaa
    10021 aaatcttttg gattgttggt ttttttgtga gtatattttc ctattttttc aataatgtat
    10081 gttatgctaa gttcgggcta tattttatac aaaggaatga tgagaatgga atttattggt
    10141 attttatgcc tgatattgat ttcaacaacg attggctcac atatctcgcg gagattaggt
    10201 atcccagcag tgattggaca attacttgtt ggtgttttgc taggccaagc tggattgaat
    10261 tgggtcaaac ctgatattct agtccatgac ttttcggaga ttggtgtgat cttattaatg
    10321 tttttagctg gtctggaaag tgatttatct cttttgaaaa aatatttccg acctggtatg
    10381 ttcgttgctt tgcttgggat catcttcccg gttgtttttg gctggttgac aggcaaagca
    10441 ttccaagttt cgggaaatga agcgattttc tttgggatta ttcttgcggc aacttctgtc
    10501 agtatttcag tagaagtatt aaaagaacta aacgtagtga atacaaaaga aggatcaaca
    10561 attttgggtg cttctgtggt agatgacatt ttagtcgttt tagttctaag tttcagccta
    10621 tcttttctaa cgggggaagc ttctagcaat gcttctttac cgcttgtttt gattgaacag
    10681 ctaatttatt ttgtttttat tttcttatta gtcaaatgga ttgcgccttt cctactttca
    10741 tttgcagaaa aagtgtacgc aaattcttct attatcatcg tatcgctagt tatttgttta
    10801 ggaatgtctt acgtagcgga tcttgttggg cttagctctg tcattggtgc tttttttgct
    10861 gggatagctg tcagtcagac aaaggttaaa gaagaagtat accacaatgt agaggcttta
    10921 ggatatgcag tatttatacc agtatttttt gtcagtgtag gattagaagt tgatttttct
    10981 gactttagtg aacaactact ctttatttct atattgacgc ttgtagcgat tttgaccaaa
    11041 ttgcttggag gttattttgg tgctcagatt gccggttttt ctcaacatag ttcattgatg
    11101 gtcggtgctg gtatgatttc gcgtggagaa atggctttga tcattttaca aattggacag
    11161 caaagtcaat tgatcgaacc acagtactat tcacctttag ttatcgtgat tttattaagt
    11221 acacttattt ctccacttgt tctcaaatat tttacaaaaa aagtttatga agtgtgagta
    11281 aatcaaaaag aagttgtgaa agagacatcg tgaaaaccgt ttatttgaaa aaaggactgt
    11341 gacgataacg ccacagtcct tttactgtac ttaaaaagca attttttatt gataattttg
    11401 gttgaggtat tgcaatagtt ccgatgctcg tttggttgct gaatcatcat cttctttatt
    11461 ttccaacatg ctgaccatca tataaccttg gttatcaaga ttgaaagcaa acaagaaact
    11521 gttctcttgc ccctttacat cctgtttttc tttgatttcc gctgtaccag ttttcgctgc
    11581 caacggaatc cctaaagcag aaagagaatg tgctgtacca tttacatctt gcacaacttc
    11641 tctcagatct ggcacgatcg tttgtactgc tgtttcgctg atgacattct tcttatcttt
    11701 tgtctcttta tctgcaatca atttaggata gacaagtgtg ccattgttgg taaaaacaga
    11761 atacattgct gcttgctgga taggagtaat tagaagttcg ccctgtccat atccagtatc
    11821 agctagcaag atatctgagt taaagctatc ttcattagaa atttgtgctg gattcatact
    11881 aattggcaaa tcaaggtctt caccaaaaat gaatttatct aagcctgtac gaaatttttt
    11941 ctcacccatt ttcaacgttt cttgtgccgc atatatatta tcggaataga tcaaagcagt
    12001 ttttaagtct acttgtgata catcactact gacacgcgtt gcttgataag atccccaaga
    12061 actgtctttt tgccatttaa gcccgttgat cgtcaacact tcatttggat cgatagtgcc
    12121 gttgtcgaga ccgattgctg ctgtaatcat tttaaacgta gatccaggag cataacctgt
    12181 cgcaaatcgg ctgatgaatg gatgttcagg attttcttca taagctttgt aatcttcttg
    12241 tgagatcccg tttgtcattt tgtttggatc atagcttgga gagctagcaa gcgcaagaag
    12301 atcaccggtt tttggcgttg tcgcaacagt tgatccagct tttcctccta gactgtcaaa
    12361 agctgttttt tgtgccttcg catcgattgt caatttgata tctttgccat tttgaacttc
    12421 atgttcgatc agaacttttt tctctacacc gtctgcatct gtaatgctta atttcccacc
    12481 tgttgtccca cgaagatcct tatcaaaagc catctccaaa cctgagcgac cgatttttcc
    12541 attactgctt aattctggat tcttatcaat atcttctgct gtgatatctc ccacgtaacc
    12601 aatcagctga gcagctgctt cacccaaagg ataatatctg ccgtctactt cttgaatggt
    12661 agctccagct ggaagttctg gcgttgctcc atcaatgatt ttcaatggga caaagtaatc
    12721 gggttgtacc cagctttgtg aaatcgcctg attgatagca tcttctgtta agtcgaagga
    12781 ggaagcaatc gctttgatat tggctgtttt ttcgcctcca tctccaagtt tacttggaac
    12841 gactcctaat tgttttagtt tgcctgttgt tgctaatggt tcaccatttc gatctataat
    12901 attccctctt gctgcttctt gtgtggtcag gcttactttg tcatttcctt ccatttctgg
    12961 aaaaaccaag ttgggttgcc agttgatcgt ggtttgacca tcatttctgt ctaatgttcc
    13021 tttataagaa agatctttca attcacctaa tgaggtattc atctttgctt tatatgaaaa
    13081 actgtaagta gaatcgtctt ttttatctac ttttagattc gatatctgaa gtcctttgac
    13141 atcagcagca ccgtatatat tctggtattt atctaagatc tctttttcag ataatgcact
    13201 tttatttgcc gccttttttg atgtcatttc tgcagctttg ttataatctc ccttgttcaa
    13261 agcttggaca aattgctcaa ctgtcttttc tccagcttct actgcttggg tttcttgata
    13321 gtgctggtag taaaaataac cgccacccgc agctgctatt aagatcactg cgccggcaat
    13381 ataagcgcct gttcgatttt tgccgtgctt gtcacttctt ttcatttttt cctccttctc
    13441 ttgcttgtca ttcctttatt tcactattta tacctgttta ttctaacata tgtctttgag
    13501 gaaatccata ccacaatcca gaagcacact ttctggaagg ttcgtttgga tactaccaag
    13561 taatttccca accgctttgg gtagcttgaa tattgaagtt acgtctttca gctgctgaga
    13621 gatagctttg atcacttgct gctgccttct gatcctcccg aagtctcctt ctttgtcttc
    13681 ccgaaatcga gcatactgga gtaaagtatt accatccatc acttgctctc ctttgtttat
    13741 atatacgcca tctaaatcta gagatttttc tgcatcgatc ttgactccac caggaaacag
    13801 ttcattaacg caatcgataa aatcttgaaa tgtgatactt gcataatagg gagcttcaaa
    13861 tttgaaattt tcctttaatg tttgatttaa taaatcaact cccccataag agtaagctgc
    13921 atttatctta ttcattccgt aacctggaat atcgacatat gaatctctca tgatagagat
    13981 cagttttgct tgttgtgttt tctggttgta ttgtgcaacc agaagtgaat ccgagcgacc
    14041 gttttcgtct ttcgaacgag catctgaacc aatcaaagcc acatttagtg tggtagggat
    14101 acttttcggc tctacctttt tcacagttga actctgtttt gcagacgaaa atccatggat
    14161 aaactcaatc gaaaaatagc caactactaa tgtaagcagt aacaaaatag ataatatgat
    14221 tttttgagat actttcattg cgaattcctc tttctaaaag aacctctcgc ttgaaaaata
    14281 aaagattgta ctttatgata attcaaactg agagtttctc agtttctctt tattgttttt
    14341 caggtcgata gagcgccaac tcttcggcct gtatctttga agaaatattc aacgtgattc
    14401 cgattcctaa agaaaggatc agataacttg ttcctccgta gctaacaaat ggcaatggca
    14461 caccagtcat tggcatcagc cccgcgatac ttcccacgtt cattatcgtc tgaacgaata
    14521 gcaaagttcc gaatcctaaa agaaacaatc cagcttgctg atttttacag cgactgctta
    14581 aacaaaagat tctcatgcat aatgagaaca ataagaaaag cacacacaat gcaccgatta
    14641 gcccaagctc ttctgtaata atcgaaaaaa taaaatctgt ttctgtctct ggaagatacc
    14701 ctttttttgt gatactattt cctaatcccc gcccaaatat gccaccatta tatagtgcat
    14761 aaaacgaatg ggtcatttga tagcccgcac catgactatc gataaaagaa tctcttaaag
    14821 tcgcgatcct ttcataagca tgattaaaca tctgtggaag ccaaccttta tttcctaaat
    14881 ataatacccc gcctgctgct ccaatcagca atgcagaaaa agtaacgatt agatagatcc
    14941 cttttttaaa tggaaccgcc gctgcccaaa atatgaccca cgcaatcgaa aggatcatca
    15001 atgctccagc aatctttggc tgaaataaaa tcagaaaagt tatacctact gtaatcagga
    15061 atggtttttt tagatctttt gggctactat ttccgtcacg aaaaaaccag cttaaataaa
    15121 aaatcaaaaa aagatttgcc agttcagaag gctggaattg aatgccaaac agagagatcc
    15181 aacgttgtgc gccgttgaca gtgacaccga atatccctat tcttactaat actaaaaaga
    15241 aaatcgataa ggctaaaccg tatcctgcta ttttagggtg aagtaaatag tgtagtttga
    15301 ctgaacgagc taagaagatc acgctccaac tcaaaaatat gaaaattagt tgacgcaata
    15361 atagactttt tgtattttca tcggcttgca ataaacgata agaactggca ctgtatactt
    15421 ctaaaagacc gatcatggac aaagtcaaat aaggacccaa aatccaccaa tcgatctttt
    15481 ttcaccgcat ctcctcctca cacagcaatc cacttgtcag gtgaggagcg agtttgtctc
    15541 atcaaataag tcagtcacaa aaatataaag aaaaggtcag aaaccttttc gaacaagaat
    15601 tctgacctag catcatctca tcgtccgagt tttagcacta tacaacgtaa agaaaacatt
    15661 cttagctatt ggaaaaagcc ttaatccaac agtttctgtt ttacccattg gcgactttcg
    15721 acgtttctgg gcaacagcct atgtttgtat aggagcctca cctaacaagg ttattactat
    15781 attctattta cgagttcatg atagactttt cctaatgatt aggcaatgga atttgaaatg
    15841 aaatagtgaa atttaacaat ctttaatatt ttttagaaaa aaataacaaa taagttgaaa
    15901 gaatcccgtt gcaaattctt tcagttgcac caggattctt tgataatttt ttagttttat
    15961 gtgttttttt atatgttttt tcacttttcg gaataaatat ttatttgtta aagtaattaa
    16021 acaatccgct aatcgtttga tctggcattt ctatttcctt accatctaaa gtaacagtag
    16081 taacgacaaa atggtcttgc aatggcggaa cttctaagta ctgcccttgc agaaaatcat
    16141 tcactgtttc atattctttt ttcatttctt ttgaatctgt cgatggttta tagcttattt
    16201 ttaacataac tagtgctcct ttttattcgg gacgtgcaat cgtgaaaatt tctcctattg
    16261 ctgcataatc atttcctgcc aaacggctta cagcattcaa ttttcttgga tcgatccttc
    16321 ctgtcttatc atcatagact gtttcattta aatgatattt ctgtacttct agcaaaaata
    16381 aatcagctgt tactttattt ttattccgta tctcgacatg ttcatataag gatgtttcaa
    16441 aacgaacttt tgcttctgtc aatgctagta ctgaaatgat tactgaatca gttaaagtca
    16501 agtcagtcag gtacagttcg ctttcctcat taggcagatt ggctgctgtt ttatttactt
    16561 cttctacgtt gtcctcatca aggatatgga taacagcttc tcttgtctca agaatattct
    16621 ttgctgtgtc ttttactgca tcttgttttc tctggatcgc caaggataag atgggtcgat
    16681 tcgaactgac gatattgaag taactgaagg gtgcgatatt cactatatct tttgctgatt
    16741 tagtcgttac tactgctact ggtctaggga tgactgaacc aatcagcaac ttgtaatttt
    16801 cacgttctgt caattctgaa ggactccgtt caaacatggt ttcgccttct ttctataaaa
    16861 ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa tgcttttttc atcttcttag
    16921 aatctgcttt aaactatcta tcaatttttc ttttttgtca agaatctgtg ccgtgcgtgt
    16981 tttttagtta tttgctttac ttgcggcttc gacatatctt ttagtgatca attgcggacg
    17041 ggtgataata gatccaacta cgactgaaaa agcacctaat tcatatgctt tctctacttg
    17101 ttcaggagag tcagtatgtc cttccataat tactgggata tcgacttgtt ctatgatttg
    17161 attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa tattctgtat agcctgctaa
    17221 agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga agcgcttcct caaaagtcga
    17281 aatatctgcc atagctaacc gaccgtgttc gtgaatcatt tggacaagtt cagcagttgt
    17341 tttttctgtc ggacgtttac gagaagttgc atccagtgca atgatttctg cttgcgtctc
    17401 caaaagttct tccacttctt tttctgtcgc agtaataaac acttctgacc catcgtaatc
    17461 tcttttgata atccctatta ccggtaaatc aacgattcgc ttgatttcat tgatatcttc
    17521 tgtcgtattt gctcgaatac caactgctcc gccttctttt gcagccaaag ccattctccc
    17581 cataataaaa gaactgtgta gcggctcgtt ttccaaagct tgacaagaaa cgacgactcc
    17641 gcctttgatt tgttccagtg tatccacaat atctcctcca gtaatgttat ataaaaatac
    17701 agcttcatat aaaaatgtaa cccttttcta ttcaggagac aataatgagc tatttctttc
    17761 agcagacctt tcttactaaa agaaaattac ttcaaattct tcgtatacga ccagcttctc
    17821 ttctgtaaat tctatgttca acgcttcttt ataagcatgt ttaaaaaact cagcatggac
    17881 tttccgccaa taagctaatg tccgatcccc ttctccttct ttccaagcat gctctttcgt
    17941 gacttcacta aaaggcaaga tggtcacatt tatatttttg atgatacaaa ctggttcatt
    18001 ttttccattc aggaccacgt ctaattgacc aacttttggt aaaggctcat tctcttgttt
    18061 atacagatcg taaccacttg atgtaccagt ctttatcttt tttacaacta gttcgctcaa
    18121 ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa cctaattttt ctgattgata
    18181 actaggatat ttttcttgga actttttgac atattcttct acttgctgat acataacatc
    18241 cacccctttg ctttttataa tgaaaaaacc atccagatac tagtataaac aattatctgg
    18301 atggtgagca taatgaatta ttgaatattc aagacacgca gactatcttc aacgatttgt
    18361 tttggtgtta aatgataacg ttcataaagc tgccctacag gaacgctatc cgtgaattct
    18421 ttctctgcac caaaatttaa tacgttcatt gaagaatcac cataataacg actgatattt
    18481 tcgccaaatc ctcctgacaa actaccatct tctaatgtga tgactaattc gtgtgtttct
    18541 tttaatttat ctaatgtttc tacgtcattt tctgtgatcg ttcctggatt gatcaacgtt
    18601 gcttcgattc ctttttttct aaaagctttt actgtttctt ttcctaattc aagaaaaccg
    18661 cctaaagcta aaattgctac ttttcctcca ttatttactg tttgataggc tggaatacta
    18721 taatcatttg cgatagctgt accatgttct acaccatgtg taggaattcg gattgctaca
    18781 ggctgcgctg tttgttcgaa cgcccaatct agcatagaga tcatatcctc ttttgttgct
    18841 ggagcaagat atttgatgtt cgggatactt gataagtaag gaatgtcaaa tgttccttgg
    18901 tgtgttcggt cactagctga gatcgttccg ccaccgacta aaataatcac tggattctcg
    18961 ttgatcgcta aatcatgtga taattgatca tatgcccgtt gcaagaaagt acttgattgg
    19021 aagacgactg gacgtactcc ctgagaagcc aaagcagaag cgaaagtgat agagtgctgt
    19081 tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt cttggaatgt tttcagtcca
    19141 aaggcgcctg gtatcgctgc attgactgcg gcaataggag ctgtacttgt ttgcattttc
    19201 ttatccaaat aatccatgat gatcatatta aatgtttctc cagaagttac ttgctttgcc
    19261 tccccggtgg acagatcaaa tggtacatgc caatgatatt tcattttatt atcgattgcc
    19321 ggctgataac cgcgaccttt ttccgtatgg acgtgaagta caatcggatg atcaatatct
    19381 tttacttctt caaataaacg gatcaccgtt tccagatcgt ttccattatc cacgtaacga
    19441 taatcaaatc ccattgcttt aaataggtta ttgctgcttt taccgttggt ttgacgaagc
    19501 tcagctaagt ttttatataa actgccatga ttctcatcaa tagacatctc attatcgttt
    19561 actaatacga ttagattgga ttgtaaaaca ccaccattgt tcaatccttc aaaagctaag
    19621 ccaccactta gggagccgtc accgataaat gctaccacat ttcctgattc tttcttcaga
    19681 tctctcgctt tagctaaacc tgttgctaag ctgatagatg tagatgtgtg ccccactgta
    19741 aaatagtcat gtttactttc atgttggttg gaatatccgc tcacttcacg gtaatgtcct
    19801 ttttcaaaac cattttttcg accagttaaa agcttatgac cataagactg atgggaaaca
    19861 tcccaaacga ttttatcctt tggtgaatca aatacataat gaaacgcaat cgttaattct
    19921 actacaccta gattcggacc aacgtgccca cctacatcgc tgtccttttg caagattttt
    19981 tggcgcatct cttctgccaa ctgtttcatt tcctcaacag ataatttttt gacatctgcg
    20041 gggctattga ttgcatccaa tacatgtaat actgtactca taatacattc tctcctccgt
    20101 actatcttct tttaatgaaa atagatttgt tacactttat ctattctact acttagagtt
    20161 gactctaagt caatagcatt ttaaaaaaat ttcagagaaa gcttgttata taagtagatc
    20221 ttcgcacgaa tcaggactca aataaaaacc ttctacagac aattaaaaag ctttgtagaa
    20281 ggtctcctaa ttaaggtctc atcaagaaat atgttttttt aataggtgaa gttcctgagc
    20341 aatatgatac aaatggatgt ttgcaccttt taatgagatt ggatagatct ttgtcttttc
    20401 agtttctaat ccattttgat agctagcagc atcgcacaaa accactcttt ttttcgcaca
    20461 tagacattca tcaggcaatt cttctaatga acgaaataaa ataggattta tttcagcgat
    20521 gccttcttcc gcatacaaat gaaattgggt tcgataaaaa aattgtttga ctgaactttc
    20581 tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt tcactgttga tcaaagaaag
    20641 acttttgacg aattttcgaa tgaaattttc attccatctt agttggattt gatttttctt
    20701 tttccactct tccaatattt ctacaatttt tccgtacaga agttgttctt tttctgatag
    20761 aagatagatc ctttccggta aaaatacttg tccatctgcc cacaacgtac ggacaaattg
    20821 taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg atttcagcga tactaataat
    20881 ctgtaaaatc aattcattca gtgataggtc attgagaaca aaatttttat aaacaacttc
    20941 gaaatctttt cttaacagct ccatgttctc attggtataa tttctactgt tgaacaaaga
    21001 aaaaatataa tagacatctt cttcttgtaa gtctagaatt cctttttgct cttccaatcc
    21061 ttgacagact aattgaaata acggcttcgt ttctgcgctt ttcttatctt cttctttcag
    21121 tccaagggga aatgcctttc ttccgattgc gatctctatt tctcgtatga taaaggactt
    21181 ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct acataatcaa tcaactcgtt
    21241 acaagcttct tctacatacg gatttttatt ttcatagcct tcccaattga cataacgtac
    21301 taaagctagc aagatgttcc gcgagtcaaa ttctgggata atgatttctt tatttttcaa
    21361 ataatcattt tctttaaaaa aatccaatac gatttttttg attgtatata ctttactcaa
    21421 agaaataaat tcaagatcag ccagttctgt actcgtgaat ataccattaa aataataact
    21481 taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag aaagagcaat
    21541 accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt ttatacaata
    21601 ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag tcacccttag
    21661 agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt cacacagata
    21721 cacttttcta aatatatttc gctcgatgta attttccagc attcctgata cacgcctcca
    21781 gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt cctttttatt
    21841 tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc aaacaagctc
    21901 acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc cagcctcttt
    21961 caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt tcaacaattg
    22021 gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac ctatgccgta
    22081 acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag gtacaccaac
    22141 atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa ttgatttgac
    22201 gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa ttcctttatt
    22261 tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg agcattgtct
    22321 gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac ctttcatata
    22381 gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga tttcttcatt
    22441 caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc gctcaaattt
    22501 ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg cattaacaaa
    22561 aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat tgaaggcaaa
    22621 accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt ctttgattgc
    22681 taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat cggcgtctcc
    22741 tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt gtttgggatc
    22801 cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat agtggaaatt
    22861 ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct ttcatttttt
    22921 atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga ataactaaga
    22981 cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca gctgcgatac
    23041 tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga cctaaacaag
    23101 caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca agactgtcat
    23161 ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc aggtagatta
    23221 caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg cttaatgtaa
    23281 agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaatga gcaggaacac
    23341 cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct gctacgtgaa
    23401 gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct aaacaaacaa
    23461 gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt aataggaatt
    23521 ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact gtagtagcga
    23581 cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca aacattgcta
    23641 atggataatc ctttttaggg ttttccattt catttgcatg actggcagat gtttctacgc
    23701 ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc gaaaaatcag
    23761 gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg acataatgga
    23821 tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa ccgatccgag
    23881 caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc cagaaaataa
    23941 tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata cccgttgcat
    24001 aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt atctcaaacc
    24061 attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg acccaagtat
    24121 agacaccgcc tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag
    24181 ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg
    24241 cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa
    24301 agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt
    24361 tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt
    24421 cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg
    24481 ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga
    24541 agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa
    24601 tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt
    24661 gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca
    24721 gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa
    24781 cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca
    24841 gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt
    24901 gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc
    24961 ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct
    25021 ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga
    25081 ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc
    25141 agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt
    25201 ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca
    25261 tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga
    25321 agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat
    25381 cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg
    25441 agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa
    25501 aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg
    25561 tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag
    25621 tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat
    25681 aaaagacaga caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa
    25741 taaagacaaa aaataaacgg tcttcttttt atagaaaacc gtttattttc gtcttttttt
    25801 atttttaaag atattttttc ataaggaata aagaatactt catataaaat atataaaaaa
    25861 gaaccgcagc gagtgtcgct acggttctca aatttgttct tcatttcaac ataagttttt
    25921 cttcaatttg cttataattt acgtgcaaag aatgaaacga ctaatactaa aatgattgca
    25981 ccgatcaatg atggaatgat agccattcct gcaattgttg gtccccaagt tcctagtaaa
    26041 gcttgtccaa ttgctgaacc tactaaacca gcaatgatgt ttgcgatgat ccccattgat
    26101 gatcctttat tcgtgattgc tcctgcaatt gctccaatga taccgccgac gattaatgcc
    26161 caaataaatc ccattttaaa ttcctcctaa ctgacttctt acttcttatt gtacacgtgg
    26221 ttcgttattt tctttgactt tttgtgcacc tttgcctaca gcagattttg ctttatctgt
    26281 ttggcgtgaa gcaaattgtc ctgtggattt tgctgcttct gttactttgt cttgtactgt
    26341 ttcttgatct ttttggtatt cttcttgtgt tttgatgtct gcaacattca cattcacttc
    26401 gataacatct aaatgtgtca tattgtttac ttcagtactg atcaattctt tgatttggtt
    26461 gtaaatatct tcgatgtctt taccgtattc tacgatcaca tctagatcca cagctacttg
    26521 tttcttacct acttctgtat tgatacctgc tgttacgtta tctgtgttga ctaatttttc
    26581 agccatattt gagaaaaatc cgccatctaa tgtcaataga ccatctacat ctttcaatgc
    26641 ataaccgatg attttttgga tgactttgtc ttcataagtc aattcaccat tcatcatgcg
    26701 gttttctcct ttttcatttg ccaaaactga tccatttgtt gtattcatgt ttttgttttc
    26761 catctttgtt tcctcctatg atttaaaaaa ttgatcaact attcctgact gtttcaaata
    26821 aaaaccgatt cctgcgccaa ttgctgtcat gattatgatc aacaatgtct tgaaaaatcc
    26881 aatcgttaac cataaaacag ctagcagtag tccaagaccc ccaaagataa tagggagctt
    26941 gtatgctgaa aatagttctt tcattatttg gccctcctat tccactcgag agtgcttgta
    27001 gtctcttgta tcttcttgtt cttcataacc gctgtatgtg actgccactt tgaccgtttc
    27061 ttttgttccg agtactttag ttacttcttc ttcgatatct tgcatcaata cacctgtttt
    27121 ccctactaat gaagaagttc gtttcaagtc tccgttgaca tggatatgga ttcgattttt
    27181 cgttgaacgt atccgtactt taggtgaatg aatgaactct tcttcgtgta aatgggaacg
    27241 taccaaccct tcgatcgctt ttttgtcgat tgttagtttg ccatcttccc gtttcataac
    27301 gaaagttcct cttgactttg gatagaaaag aacgactagc aagatgataa tagcaagaac
    27361 ggcaaatact acagctgccc agaaaatata ttgttgcatg tagtaattcg ttaaagtaaa
    27421 gaagcgaaat cgttccaatt gttcaggcat aatatacggc gcattgatca acgcaatcgc
    27481 tagaaatact gaaagtagca gtaaactcat gataacaccg attattttta accccttatt
    27541 cattttccac ctcctataaa cttattttta ccctgtaaat aactctaccg acaatcacag
    27601 tatagatagc gaacagttag catagcatca gtaaataaag cgctaccaaa cattgctgga
    27661 tcataacggc gttttattgc cagtctgata ctgaaataac aattgcttac ttattctcta
    27721 tttacttttg attgctggct atacttaaag gatattattc aagattaaaa attgcaaaga
    27781 ttatgctcag ttattattac aagattctat ttatttatta aattttttcg gagaattaaa
    27841 aaatacaata aataacttta gttactttaa tgcgttctta ctttattttt atattatcat
    27901 ttttcttatt cacttctttg gtttatacta ttcttataaa taaaaatgag gtatgtccta
    27961 tgatgaaaag attgtactat tcattgatca taacgattgg ctatttgatt gtatcaaatc
    28021 taggaaatat ggtatttggt atcagtaaag aattcagttg gactactaca ttatgggaat
    28081 ctctattctt tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa taaacatgtg
    28141 ccatctatct gttggcacat gttttaggat ttatggtact ttttattcgg caatccattt
    28201 tatttttcta cttcttctta atacctctaa cacttttgat ttataaacta atccaatgca
    28261 tctacttgcg cttattaagc tatttcagct agatagtacc accccatgcg tcaatgcttg
    28321 attttccttt tgtataacat tgtataagca ataaaggaca aataagtggt gattacacca
    28381 aaccattgaa taagaaaaat tctgatacgt aaataaacat gaacttgaaa aaattaatga
    28441 atcggttagc tactacgtag ttgtcttttt tctcatacag ttctgttaac ttacacttct
    28501 cttcgctaga taataaaaaa tcctccaact gtattgtcca atataatccc aggcatctaa
    28561 actagcatta tagaagagac acacatagaa tctaaaataa ctggttttaa atgtttctta
    28621 caatcaaaaa aagctgttaa atgcactcca caccttcata ctctatcatt ttttataaac
    28681 gatatatttt tattgttttt cgataaaaaa tagttgtttt acaataatta ttctgctata
    28741 ttttagctat caaagataaa agagggataa aaatgaaatt taaaacattg ctattaaaat
    28801 ttgtcgtttt aggtataagt gccgtattta ttttgttcgg cgtacttttt tatactcaga
    28861 tcgctacaag cgaaaaagga ttcaattttg attggcagac cacattattt gtttctgtca
    28921 tttactttgc ccttctccta gggatcgtta ttgcttattt cttattgaaa ctattgaaaa
    28981 aaatcgaaga taaccaagct ttttccactc aaagcttaaa catactaaaa aaaatcgaac
    29041 gaaccatttt gtttattggt ttcgcctttt ttggcttact tcctaaattt atgaaagtag
    29101 cggatttaga cgatgctcca ggtttgatgt tggtaggaat cgctgtcgta ttcattcctt
    29161 ttgcagtata cacattaaca agcgtgctgg aaaaactgct tgaacatgcg attttactta
    29221 agacagatca tgatttgaca gtgtgaggtg cgacatggca attatcgtta atttagatgt
    29281 agtattagct caaaaaaaaa tgagtgtgac tgaattgagt gagaaagtgg gcattacgat
    29341 ggcaaacatc tctattctaa aaaatggaaa agcaaaagcc atccgttttt ccactctcga
    29401 aaaaatctgc caagttttgg agtgtcaacc aggtgatttg ttgacctata aaaatgactc
    29461 gaatactata ccagaagaat aaaataaagt agaacaaaca gtaaataaaa aataatctga
    29521 atgatagatg tgtgagactt ttagaagtta tttacgtcta ttattcagat tttttatttc
    29581 gattgaatac cgttttatct ataaataaga tatgtactga ctcatacaaa gggggccttt
    29641 tcttctccat gtactatcac ttttcctgat acaactggtt tcctgttacg ttgtagttgg
    29701 atctgctttt agtataatac tttattcatt ccaccagact gatagcagtt tgagcagtct
    29761 aaataatcat gtagtattac aggccattct gtttcatacc ttgaagtgct taaaacttca
    29821 gctaatggcc cattgtgtta actcttactc cattcttttt tagtaagctc ccatatatac
    29881 tgtaaagcct tctatatatt atgtcatgtt gctttttaac atttactatg tagttttgat
    29941 aaaatgattt gaataacagt gatggacaat tcttttttat ttttaacatt attttgataa
    30001 taatg
//
LOCUS       DACCSK010000022        28900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCSK010000022 DACCSK010000000
VERSION     DACCSK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.022"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(5..586)
                     /locus_tag="IVP39_000862"
     CDS             complement(5..586)
                     /locus_tag="IVP39_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7585950.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IVP39_000863"
     CDS             complement(769..1395)
                     /locus_tag="IVP39_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7585951.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IVP39_000864"
     CDS             complement(1417..2748)
                     /locus_tag="IVP39_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7585952.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IVP39_000865"
     CDS             complement(2903..3304)
                     /locus_tag="IVP39_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7585953.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IVP39_000866"
     CDS             complement(3390..3596)
                     /locus_tag="IVP39_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585954.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IVP39_000867"
     CDS             complement(3691..5193)
                     /locus_tag="IVP39_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7585955.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IVP39_000868"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IVP39_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7585956.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IVP39_000869"
     CDS             complement(5764..6381)
                     /locus_tag="IVP39_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585957.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IVP39_000870"
     CDS             6602..7975
                     /locus_tag="IVP39_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7585958.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IVP39_000871"
     CDS             8050..9162
                     /locus_tag="IVP39_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7585959.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IVP39_000872"
     CDS             complement(9234..10871)
                     /locus_tag="IVP39_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7585960.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IVP39_000873"
     CDS             complement(11039..11647)
                     /locus_tag="IVP39_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7585961.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IVP39_000874"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IVP39_000874"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7585962.1"
                     /translation="MTLKEEIIQESKRLGINKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IVP39_000875"
     CDS             complement(13201..13953)
                     /locus_tag="IVP39_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585963.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IVP39_000876"
     CDS             complement(14094..15044)
                     /locus_tag="IVP39_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7585964.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IVP39_000877"
     CDS             complement(15045..15884)
                     /locus_tag="IVP39_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7585965.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IVP39_000878"
     CDS             complement(15881..16627)
                     /locus_tag="IVP39_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7585966.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IVP39_000879"
     CDS             16865..18235
                     /locus_tag="IVP39_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7585967.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IVP39_000880"
     CDS             complement(18290..19186)
                     /locus_tag="IVP39_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7585968.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IVP39_000881"
     CDS             complement(19467..20144)
                     /locus_tag="IVP39_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7585969.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IVP39_000882"
     CDS             complement(20235..21140)
                     /locus_tag="IVP39_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7585970.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IVP39_000883"
     CDS             21315..22769
                     /locus_tag="IVP39_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7585971.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            22785..23816
                     /locus_tag="IVP39_000884"
     CDS             22785..23816
                     /locus_tag="IVP39_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7585972.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IVP39_000885"
     CDS             23823..25010
                     /locus_tag="IVP39_000885"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7585973.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IVP39_000886"
     CDS             25166..26548
                     /locus_tag="IVP39_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7585974.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGRGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26675..26905
                     /locus_tag="IVP39_000887"
     CDS             26675..26905
                     /locus_tag="IVP39_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585975.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27105..27773)
                     /locus_tag="IVP39_000888"
     CDS             complement(27105..27773)
                     /locus_tag="IVP39_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7585976.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27962..28759)
                     /locus_tag="IVP39_000889"
                     /pseudo
     CDS             complement(27962..28759)
                     /locus_tag="IVP39_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8042 a         5907 c         5300 g         9651 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgttaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagatcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagaggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    26641 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    26701 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    26761 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    26821 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    26881 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    26941 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    27001 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    27061 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    27121 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    27181 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    27241 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    27301 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    27361 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    27421 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    27481 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    27541 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    27601 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    27661 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    27721 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    27781 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    27841 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    27901 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt
    27961 tttacacaac cgttacggtt gttttacgaa attggacgag actttgccag tttaatttct
    28021 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
    28081 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
    28141 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
    28201 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
    28261 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
    28321 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
    28381 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
    28441 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
    28501 gatggatccg atttttggct actgctccaa cttttcctcg ataagaattg tagcgacgtg
    28561 atttacgcgt gtatgccgtt acttttattc ctagcttctt gatcaagcgc tgaactttct
    28621 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
    28681 ccttgtgctt ctcccgaatt ttaagcattt ccgcttcaat ctcttgatct ggatttttac
    28741 ggtccaatcg cctttgccat ccaaataatc atgaacgatc tttaatttaa gttcaaaagt
    28801 atatttaacc ataaaaaata ccgacctcca aaagttagat tttttggtct aacttttggg
    28861 ggtcggtaca caaatcgtgt cacagtccct tttttgaaaa
//
LOCUS       DACCSK010000023        28641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCSK010000023 DACCSK010000000
VERSION     DACCSK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.023"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(208..972)
                     /locus_tag="IVP39_000890"
     CDS             complement(208..972)
                     /locus_tag="IVP39_000890"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7585977.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(973..1920)
                     /gene="prmA"
                     /locus_tag="IVP39_000891"
     CDS             complement(973..1920)
                     /gene="prmA"
                     /locus_tag="IVP39_000891"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7585978.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQIVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(1933..2421)
                     /locus_tag="IVP39_000892"
     CDS             complement(1933..2421)
                     /locus_tag="IVP39_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7585979.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(2387..3058)
                     /locus_tag="IVP39_000893"
     CDS             complement(2387..3058)
                     /locus_tag="IVP39_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7585980.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            3196..4482
                     /locus_tag="IVP39_000894"
     CDS             3196..4482
                     /locus_tag="IVP39_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7585981.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            4554..4748
                     /gene="ssrS"
                     /locus_tag="IVP39_000895"
     ncRNA           4554..4748
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVP39_000895"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4826..5110
                     /locus_tag="IVP39_000896"
     CDS             4826..5110
                     /locus_tag="IVP39_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585982.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            5183..5662
                     /locus_tag="IVP39_000897"
     CDS             5183..5662
                     /locus_tag="IVP39_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7585983.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            5885..6028
                     /locus_tag="IVP39_000898"
     CDS             5885..6028
                     /locus_tag="IVP39_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7585984.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            6606..8189
                     /locus_tag="IVP39_000899"
     CDS             6606..8189
                     /locus_tag="IVP39_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7585985.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNPYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDESTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(8626..9810)
                     /locus_tag="IVP39_000900"
     CDS             complement(8626..9810)
                     /locus_tag="IVP39_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7585986.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(9835..10842)
                     /locus_tag="IVP39_000901"
     CDS             complement(9835..10842)
                     /locus_tag="IVP39_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7585987.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(11014..11373)
                     /locus_tag="IVP39_000902"
     CDS             complement(11014..11373)
                     /locus_tag="IVP39_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585988.1"
                     /translation="MPYGQKPISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(11345..11716)
                     /locus_tag="IVP39_000903"
     CDS             complement(11345..11716)
                     /locus_tag="IVP39_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7585989.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKTDQ"
     gene            complement(11733..12035)
                     /locus_tag="IVP39_000904"
     CDS             complement(11733..12035)
                     /locus_tag="IVP39_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7585990.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(12052..12522)
                     /locus_tag="IVP39_000905"
     CDS             complement(12052..12522)
                     /locus_tag="IVP39_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7585991.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(12497..12775)
                     /locus_tag="IVP39_000906"
     CDS             complement(12497..12775)
                     /locus_tag="IVP39_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7585992.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(12772..13821)
                     /locus_tag="IVP39_000907"
     CDS             complement(12772..13821)
                     /locus_tag="IVP39_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7585993.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(13778..14707)
                     /gene="cpaF"
                     /locus_tag="IVP39_000908"
     CDS             complement(13778..14707)
                     /gene="cpaF"
                     /locus_tag="IVP39_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7585994.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14839..16593
                     /gene="ade"
                     /locus_tag="IVP39_000909"
     CDS             14839..16593
                     /gene="ade"
                     /locus_tag="IVP39_000909"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7585995.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(16691..17353)
                     /gene="trmB"
                     /locus_tag="IVP39_000910"
     CDS             complement(16691..17353)
                     /gene="trmB"
                     /locus_tag="IVP39_000910"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7585996.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(17401..18180)
                     /locus_tag="IVP39_000911"
     CDS             complement(17401..18180)
                     /locus_tag="IVP39_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7585997.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(18387..19598)
                     /locus_tag="IVP39_000912"
     CDS             complement(18387..19598)
                     /locus_tag="IVP39_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7585998.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(19591..20328)
                     /locus_tag="IVP39_000913"
     CDS             complement(19591..20328)
                     /locus_tag="IVP39_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7585999.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDTAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            20466..20900
                     /locus_tag="IVP39_000914"
     CDS             20466..20900
                     /locus_tag="IVP39_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7586000.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            20893..21237
                     /locus_tag="IVP39_000915"
     CDS             20893..21237
                     /locus_tag="IVP39_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7586001.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            21417..22427
                     /locus_tag="IVP39_000916"
     CDS             21417..22427
                     /locus_tag="IVP39_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7586002.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLKSSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(22496..23440)
                     /locus_tag="IVP39_000917"
     CDS             complement(22496..23440)
                     /locus_tag="IVP39_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7586003.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(23424..26144)
                     /locus_tag="IVP39_000918"
     CDS             complement(23424..26144)
                     /locus_tag="IVP39_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7586004.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(26141..27361)
                     /locus_tag="IVP39_000919"
     CDS             complement(26141..27361)
                     /locus_tag="IVP39_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7586005.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSEMVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKIPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(27642..28487)
                     /locus_tag="IVP39_000920"
     CDS             complement(27642..28487)
                     /locus_tag="IVP39_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7586006.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         8667 a         5840 c         4964 g         9170 t
ORIGIN      
        1 cggtaaaacg ggaatcggga caaatatctg tttgataatt gtcccaaaat ccgtgcagtt
       61 atacgttttt ttacggaaat gttcgctttt taaaagcttt ttcaaacgat ttttcgtatc
      121 cattgtataa ataattgttt ctattataca aattgttgac tattttttca attctttccg
      181 tcgatactct tttattaatg aagaacgtta gctgaaagaa ggattcatta attcccattg
      241 atagctagca gcacttaata aataaagagg tgctgtttct gtacgaagga ttcttggtcc
      301 taatccacaa agtatccctt tgtgttctac aaaataatcg acttcatcgc gggataatcc
      361 accttctggt ccaaagacag ctaagattcg actatctggt tttgtttcac taagaatttt
      421 ggctgcattt gatttttcgc cttgctttgc tgattcctca taagcaacca agaccacgtc
      481 ataatcatct agccgctcaa ataaatcttg tttcgtttcc aataactcta ctgatggcac
      541 gatctgccga tgggattgtt ccgctgcctc ttgagcaatc ttttcaagcc gttgttgtcg
      601 tttcatacgt ttttttgcat cccatttaac gacagaggct gatgccggga aaccaagaaa
      661 agcttgcgct cctaattcag tggatttctg aacgatccaa tccagctttt cccctttagg
      721 atagccactc gctatggtaa tcgaacttgg cagttccttt tgattttctt cttttttaat
      781 ttctttcaga taaacagcct cttcagtaat ctccgtgatt tctgcaacaa tggctatttg
      841 atcattgaat gccaaaaaaa cttcatcctc gggttccatc ctcataaccc gtacaatgtg
      901 gtgatagttt tccccttcta aaacaaaata gtcttttgat tcatatgatt cttttaaaaa
      961 ataccgctgc atctattcca cctctggttt tttcaaaata attgcatacc agtctttttg
     1021 ctggaaaatt tgatccacga taaacccttg ttctaacatg gcatccagaa ctatttgctt
     1081 cttctcttca atgattcctg aaacgatcag cgtgcctgtt tgcttcagta aacgccaagc
     1141 atctgggatc atcaataaga tgatatctgc taaaatgttc gcaacgatta catcactttc
     1201 aatctcgatt cctgtcaaaa gatcgttagc tgatacatgg acatctttgg ctatttcatt
     1261 catatccata ttttctttcg ccgaacgaac ggctacttcg tctaaatcaa aggcatagac
     1321 atcttttgca ccaagatatt ttgctgcaat acttagcacg cccgacccag ttcccacatc
     1381 tagcaatgtt tcaccaccac gcaagactgt ttctagtgct gtcaatgtca aggaagtcgt
     1441 gggatgagtg cctgtgccaa atgccatacc aggatctagt gtgataattt tttctagagg
     1501 atcttctgtc tggtagtttt cccagcttgg cgcaaccgtc agcattcgcg taatacgcac
     1561 cggatgatag tatttcttcc aggctgttgc ccagttgctt tcctctactt cactaactgt
     1621 tactttattt tttccaatag ctagaccaaa ctcaggtaat tgtgtgatac gatctttgat
     1681 aaaaggcaag atttccggta agaaagtcgt ttccggaaaa taagccatga ctagtgcgcc
     1741 ttctttcagc gatgtaaaat catttttatc taaaatctca ccatattgat ccgactgaaa
     1801 atgttcaaca tctaaagaat cttcaatcgc gacaccactc gcccctgcct ccatcaaaat
     1861 attagcaact gcttcgactg cttcactttc agtttctact tttacttcat tccatttcat
     1921 atctgtcact ccttaataac ttgggtacgg taaataagta tctgcttcat tttgctgttt
     1981 tacttctact aattctgcta gttgctgatt atcccagtga agctcgaata cagcttcgtc
     2041 atcgtcttca tccaaaaaat caagcaaatc gctttgccct tgagctaata cttcatttag
     2101 atattccact aaagaatcag ctactgattt tgccaaccct ttttttcctt cataagggat
     2161 tactgctaga taattgcgtt catcaaaaac agatttctga ggattaaaaa gaagtacgcc
     2221 gtcttcaaat tcgataattg gttcttgcga aacgatccct tctgcatcat caatctcaaa
     2281 ttgtgcttta ttttctgcaa acaagcgaac aacgatctcg atcgtatgat tttttgtatc
     2341 ccaatctaaa gctacatcat agtctgtcac ttgtttttca attatttcat ctaaatatgt
     2401 taatagatcc ttctttttca ttttctttcc tccaacccca atctttctcg atttggtttt
     2461 ttctttgtaa agagatataa ggatttcccg acacaacaaa acgcaacggc ttttctgtcc
     2521 atacaccttt gtttgggata ccgattctag gcaaagcatt gatctttttt ggtgtctttt
     2581 ttttctcaaa aactaagcgt aacgaactat ctgctataga ctgaccataa agttcttgag
     2641 gaattgctaa tgcttccact aattttcccg gaccgttgct gatatctgga ccagtttttc
     2701 cgccacgatt tttactcatt tgatcaatca ttgcggcggg ttcgattgca cgtatcatta
     2761 ctccttgagg aatcccttct ggctgagtaa tgatgttcaa aatccgatga gtatgcatcg
     2821 tgtaaaggta gatcgtacct ggtttttcat acattgcccg cacacgcggt gttcttctta
     2881 aaccatagct atgggcggcc tcgtcttctg gacctaggta tgcttccgcg tctacaatat
     2941 atccagctaa tcttccttct ggtgtgtcat gctccaaata catccccaat aaatcccgtg
     3001 ccacttctgg cgtcgtttta ttccaaaaaa attcatctat cttttgttta tccgtcattt
     3061 ttctcaccta ctttatcata cacaatatta gttggataag ctatgaaaac aaaacaacag
     3121 aaggctgttc tttctaggca tcttgctaaa tatgatataa ttaagggacg taaaaacaag
     3181 aaggaggaaa atcctatgca acagccattg gcttatcgca tgagacctcg aacgatcgat
     3241 gaagtcgttg gtcagcagca tctagtcggg gaaggaaaaa tcatacgccg catggtcgat
     3301 gctcgcatgc tctcttccat gattctatat ggacctcctg gtactggaaa aacgagtatc
     3361 gcaagtgcga tcgccggctc tactaattat gcatttcgca tgctaaacgc cgctacagat
     3421 tcaaaaaagg atctacaagt tgtagcggaa gaagcaaaaa tgagtggtac agtgatcctc
     3481 ttattggatg aagtacaccg attagataaa acaaaacagg attttctgct tcctcattta
     3541 gaaagtggcc gaatcatctt gatcggagcg actactgaga atccttatat taccatcaat
     3601 ccagcgatcc gcagccgtac acagatcttt gaagtcaaac cattaacaga acaagacatc
     3661 cagttggcag tcgaacatgc tttaagggat aaagaacgcg gccttggaca acaagcaatc
     3721 cagttagacg aagatgctct tcttcacctt tcgcgtgcaa cgaacggcga tttgcggagt
     3781 gcattgaatg ggctggaact ggctacacta tcgactcctt cagataaaga aggaaggatt
     3841 catcttacac tgtcgatcat tgaagaatgt gtccaaagaa aagctcttac ccatgacaaa
     3901 aatggagatg ctcattatga cgttatttcg gcttttcaaa aatcgattcg tggaagtgat
     3961 gtggatgctg cacttcatta tctcgcccgt ttagttgagg caggtgatct ggcaagtatt
     4021 tgccggcgtc tgatggttat cggttatgaa gatataggtt taggtaatcc tgctgctgcg
     4081 gctcgtacag tgaacgctgt attagctgct gaaaaattgg gattgcctga ggcacgtatc
     4141 ccactagcag acgtcgtcgt cgatctttgc ttgtcgccaa aatccaattc tgcctacatg
     4201 gcattagatg cagcacttgc tgatattcga gagggcaaag ctggagatgt tcctgatcat
     4261 ttgagagata gccattacaa aggtgcgaaa tcactcaacc gtggcgtggg ttatcagtat
     4321 cctcatcatt ttgatcaggc ttgggtcaat cagcaatatt taccagataa actgaagaat
     4381 gctcagtatt atcagccaaa agatacaggg aaatatgaac aggctctggg tcaacagtat
     4441 taccgtataa aagagtggaa aaagcatcct ccaaaaaact agacaagaaa acggttctgt
     4501 ttctgcgagc tctctcattg ctctttttaa attcatatgc tactatagag gtgggaaatc
     4561 tgtgatgttc gaattgtcct ctttgtgtgt tgaccgaaca tttttattga tatcttggga
     4621 cccgtcttgt gtcactgctg gtaacatgcc ttatcatttg gtagttcgaa gctacgacca
     4681 tgggacccac ctgcttgaat tcgcgggatc aatactacag gacaaacaac ggcattaacg
     4741 gagattacca tcttttaaag aataaagcaa tttgctcttg gcaaattgct ttttctattt
     4801 gttgcttatt ttggaggatt tgttcatgat taggatttta atgattatcg ccactttagt
     4861 attattattc gttagctatt atttatttaa taaacaggat atcttttttg tattgatcaa
     4921 caaaaatgat aaaaatcaag gattcctaca attttacgga gctgcatatg ctgttttagg
     4981 cgtaatgggt attttaacag cttttttcaa tcaacgcttt attgccttgg tctttttact
     5041 gatcgtaatt attgtttctg ccaccttcag tatccgattt gcaaaaaaaa ttgctgaacc
     5101 aaagcagtaa ataattctct ttttccctaa agtgttctac aataaaagag tacaatacga
     5161 ttgacttgag gagtgaatgc ttatgttaca acaatacaaa aaaatcatgg ttgccgtaga
     5221 tggttctgat gaagctgaat tagcttttaa aaaggcggtc aatgtagcta tccgaaataa
     5281 cggagaactt ctattagcac atgtcattga cacacgttct ttccaaaccg tttcatcctt
     5341 tgatggaatg cttgcagaac aagctactga aatggcaaag caaacattgg cagattatga
     5401 aagcaatgcg aagaaagctg gcttgaataa tgtaacctct gtcgtagaat atggctcacc
     5461 aaaacaaatt attgccagag aaatcccaga agataatcaa gtagatttga ttatgttagg
     5521 agccacagga ttgaatgctg tagaacgttt atttattggt tctgtttctg aatatgttat
     5581 tagaaacgca gcttgcgatg tgcttgttgt ccgaactgat ttagaaaacc aattgccagc
     5641 taaagaccac accaaagctt aacacgtaga cgacctgcca actatcggca ggtttttttg
     5701 cacaaaaaac cgtgataaaa acaaaagacg cggcaagcac tcatcggtct ttataatgtt
     5761 taaaataaag ggaaaacagt caatgagttc aagtaaaata aaaaaaagtg aacaccaggg
     5821 cgattagtcg ccctggtgtg gtttaaaacg acctaagcat gccaaagctt attggttcgt
     5881 caatgtggaa gtttccgatg aatctgatac gaaaaaatat gaagtatcga tggcggatac
     5941 cgattttatc gccaaactaa aattttattt cgggttcggg taccgcgaga aggcaagtgc
     6001 tacattcggt aaatcattca agaattgatg aaaggctacc gcttgccatt gcaaccacaa
     6061 cgatactcct gaacgcgatg ttggtgagat ttccaaagag aagagctgtt taatgcaacg
     6121 gtattaagca aatggcaaaa ggaagtcatg tagacaaagt tttttatcaa acaccatatg
     6181 atttcgcctg aacgctggtt tagctcagaa tacaatgatg tggtccacga gacattaccc
     6241 tcagtcagat aaaaaaagct ctctgacaga attacacatc gataaatatt aagtaaatgt
     6301 cagcgctagt tgaactttct gacactttca ggtacactgg aaaagacaac tacacgaagg
     6361 agggaaaata ctatgatgcg catgactaaa ataacaagta atgaaaatta ccaacgatac
     6421 catatcgcat ttagccatgc aattggtttt acagttgctg ttgacgggac aagtttgtcc
     6481 aaaacagttg aattcacgta aaatcaatgg ccatctcaag gtatttttct catgataatc
     6541 tgagtttaaa taacacaggc catggataac catggccttt ttgagtggtc tcaaggagag
     6601 agatcatgat catacaagca atttctatta aaaaaacaat cggcgggaat ccattatttg
     6661 aggatttatc ctgtcaaatt gaagctggtg aaaaaattgc cttagtcggt atcaacggaa
     6721 ctggcaaatc aaccttttta caaatcttga ccggcagaga aggtgtcgac agcggtgtta
     6781 tcagtcgcaa aaaaggctta aagatcggga cggtcgaaca agaacttaca gtttccgaag
     6841 cgaccgtcaa cccctacttg ctccattctg ccgctgagat acaggacctc aaacagcaaa
     6901 tgagtcgcta cgaagcattg atgacagaac cagatacaaa cttagagaag tgtctggccc
     6961 gttacggtga gctgcagcat cgctttgaag aactaggcgg ttacgtatta gaagaccgga
     7021 ttgcagcgat cttgcaaggc ttagggattc cacaccgtca ggaagcacgg ttaacagaac
     7081 ttagcggagg cgaacgagtt aaagtcgctt tagccaaaat tttagcagcc gatgccgatt
     7141 tacttttact cgatgaaccg accaatcatt tagatcttaa cagtattcgc tggttggaaa
     7201 actatttaca gaatacaaaa aaatcggttc tggtcgtttc tcatgatcgg caatttctcg
     7261 atcaagtcac cacaaaaaca tgggaactgg aagaaggtgc attgattgag tatcccggaa
     7321 actacagccg ttttcgagtc ttgaaagaag ctcgcttggc tgagctgaca aaaaattacg
     7381 aactgcagca aaaagaagtc cagcggttga aagtcatgat tcgtcgtttt cgtcagtggg
     7441 cacatgaagg agacaatgag tccttcttca aaaaagccaa agaactggaa cgccgcctgg
     7501 cgaaactgac actcgtcaaa ccgccacctc ccccgaaaaa tcggctgcaa agcttgtcta
     7561 atggtggaaa atctggcaaa gaagttttca tcattcaaaa cttgcaccag cagtacgctg
     7621 atcaagtact attcaaagac agcagttttg cagtctatcg tggagatcat ttagccatca
     7681 tcggcgacaa tggtgcagga aaatcaacct tactcaaatt attactcggt gaggaaaagc
     7741 cttacggtgg caccatcaaa ttaggtagta gcttgcaaat cggttatttg ccgcagcagc
     7801 tgcagtttgc cgatcccgat agccgtttgc tggcttacgc catcacattg actggaaacg
     7861 aagaagtcgc tcgacggcaa ttagccaaaa gtggctttta tcaaagcgat gtgggtaaac
     7921 ggatcaaaga tttatccggt ggtgaaaaaa tccgtttggc gttgctgaaa ctctttttag
     7981 aaaaaatcaa cgtcctgatt ttggatgaat caaccaatca cctcgacagc tatgctcgag
     8041 aagaaattga agaaatgctg caagactatc aaggaacgct cctagcagtc agccatgacc
     8101 gctacttttt gcagcaacac ttccaagaag cactcgttat tgatcaggag cagatcagcc
     8161 gccagccgct cggccaactg attgactagg acgttttttc gcaacaaaaa acttgatttt
     8221 catacctgtg ctttcacgat tcaggcaaag tacaggtgga aaatcaagtt tttattattc
     8281 gatcaatgaa tgtttcggat cggttttcta gctaaacata aagcgaagaa taaagtcgac
     8341 agcccacgag gctgatctaa aaatgttttt tctttttaca tatgtaatgt cccttataaa
     8401 gatgaatgaa aataaaatat tgggattttc ctgaaaatca ataaataaaa tgccagtctt
     8461 ttcccttttg accacttttc caagcaaaat tccctaagaa gatcctgtaa aaaagaaggg
     8521 aaattgaagt taaattacca ttttgcggtc ttctccgcaa aaaaaacaca tgcaagaatt
     8581 ccctcttgca tgcgtttcat cttttatcct aacgaatgct ttggcttagc cgcgcagacg
     8641 ctccacatca cgggcaatca ttagttcctc atctgttggg atcactaata ctcttacttt
     8701 tgattctttt gtagagatat ctacttcttc accacgcaat tcattcaacg ctggatcgat
     8761 ttcacaacca aaccaagaca tacctttgat gatttcacta cgtacatgtc cgtcattctc
     8821 gccgatacca gcagtaaaga caattgcatc aacaccattc atcgttgtca cataaccgcc
     8881 gatgtatttt cggatgcggt ccacgaaaat gtcataagct aaacgaatat gttctttttc
     8941 atagttcttt tcaagatcac gcatatcact agacaatcct gttaggccta gcagaccaga
     9001 tttcttgttc aaaacattga tcatttcatc aatatcaatc tctaattttt ccattaaata
     9061 tgggagaaca gctggatcga tatcacctga acgtgtaccc attgtcaccc cagctaatgg
     9121 tgtgaatccc atagaagtat ccatggattt acctccatcg actgctgtga tcgatacacc
     9181 atttcctaag tggcaagtga tgatttttag ttcttcgatc ggtttgttca ataattttgc
     9241 cgcacgttcc gacacataac gatgacttgt gccatggaag ccatattttc ttactttgaa
     9301 atcttcataa taagacaccg gtaagctata taaataattt tgctttggca ttgttgagtg
     9361 gaaagatgta tcgaatacag ccacactcaa aatgtcaggt aaaatctttt taaacgcttt
     9421 gatccccatt aagtttgcag gattatgcaa aggagcaaat tctgctaatt cagcaatgcg
     9481 gtccattact ttctcatcga tcactactga atccccataa gtttctcctc cgtgaacaac
     9541 acggtgccct actccggtga tttcatcata agagcttaaa atttgcagtt caatcaactg
     9601 atctaacagc atttttactg ctacttcgtg gttttcgata tccatgattt gttcgaattt
     9661 ttgattatcg ccgtacttga tggtaaaaat agaatcttgt aaaccgatac gttcgacgat
     9721 ccctttcgca actacggttt cttctggcat ttgatataat tgccatttca aacttgaact
     9781 cccagcattg attgcaattg ttttagacat atgactctcc ttctcgttga atgattacag
     9841 agcagatgat ttccagtttt taaactcgtt gaaaaattcc gtcaccttct ctggttcttt
     9901 taatgaagca agtttcacaa gcaagacttc ttttacttgt tgtgcttgtg gtcctttttt
     9961 ctgtaagatc aaaatacttt tttgcgattg tttattacgg aacaattcat ctggcaattg
    10021 aatcattcct tgaagataac cttcttcttt aaaccatttt ttgatttctt cactttgatc
    10081 cgtttctaaa aatccactcg gcatcaaaaa caaaccaaaa ccatccggct tcacatattt
    10141 cattgattgc tctagcaata aatgatgtgc ataactatgg ccttcttcgg tacttgttaa
    10201 aaactctttt gctttttcgt cattcggata ataaccgatt ggcaaatcgc tgattgcaaa
    10261 atcgacagga tctattaata attcctgcaa cccgtcttga tgaaagtatt gaacatttgc
    10321 ttgtgtcaaa tcacttgtag aagctgcgac agccaacaat gtatcatcaa tatccacacc
    10381 aattccttct gtttggtacc ccgcattcga taggtttaaa agaactgtta ataacaagtt
    10441 gcccattcct gccgcaatat ccagaatctt aaccggtgct tttttatttg tgtatagttg
    10501 ttcaataaga aaaacaaata aaaaaccgat actatcagga gtgagttgat ggttcgcctg
    10561 caaatgttct gtctggctgc ccttcaataa aagtaattga gacagtctgc gccattcttc
    10621 tggctcgaaa gaaagtttct tcaactcttc ataaagagct gtgatccgtt gcgttgtctc
    10681 ttttgtcggc actccatcca ccacacgtac ttgataatca tctattaaat tttctgcatt
    10741 ttcgacatag gcttctaaaa aggaagttcc caatgcattc tgtaaaagct gaatagcttc
    10801 caggttttga ttaaaccctt gttccatttt ttctggtaac atggtgcacc tcatttctag
    10861 atatattata acaatttttt cacctgttta attctatcga acaattgaca aaatttcaat
    10921 cgttttcact gttttttatt tgtgaaacgg caaacatttt tttattaaaa acagtgttat
    10981 aacagaaaag agggcgtttt cacgaacatt ccatcattct gtactagtat ctgtatcatt
    11041 ttttacttct tctgtgaacc gaaagataaa cggtgagact tccacctcta agaataggga
    11101 ctgttgtacc cactgatatg ttacagatcc agttgtgtag taaaaaactc cttccgaact
    11161 ttcggcaagc gtagactgtt ccgataaagc catctgcttc attatttttg ctatataata
    11221 atttttgatt cgtatattaa gatccattga caaacggtag ctttctaatg ctgccatcaa
    11281 taaaaacgta cataaaagaa ataaaagcaa gacagaaaat aaaatagagc ccttataaca
    11341 ttttctactg atcggttttt gtccatatgg catatccatc ttctccattt tcaaagtgta
    11401 cataaaaaga aattgtgtgt tctttttcca ataattgaag ctcagtgata cttgttaaca
    11461 taggttgatg tccgccggaa gttcttcttt tacgtatcat tgcctgatat ctttcgatga
    11521 tatactgttt atcctcgatc gtgtagtgca gttgctgatt tttcacagag acattgtgac
    11581 tgctcttgag ttcttcttct aattgtgcta aaaatattag ccactctttt tgatctttac
    11641 gctgcaaaaa ttcacttact tggatggttt gctgaatcaa aagattgaat gtgagcaata
    11701 gactgctcaa tatcatcagt ccaattagac attcaatcag cgtaaatgct ggaattactt
    11761 gttttttcaa tactgatcgc ttctttctcc atattgacaa acactttttc gattactcca
    11821 ttattatcga caatttggat atcgtatggg atcatttctt cccttcgctc tggtcttttg
    11881 tgatacatcc gctgcgtctt gatatcttca taaatcattc gaataaatag cagttcctcc
    11941 ccacttttct tttctgcctg aagcaaaaac agctggccaa agctcactaa gtagacacag
    12001 acagaagcga tcataagcgc tgtaagggac tccaacaaaa caaatcctgt tttattcaat
    12061 tctttttgta tagcgaccac tccctatctg gaattgatac gtaatcgtcc gctctttttc
    12121 ttcccaataa aattgataag cttttagtga actttcattc ccggttctag cagcaaaagt
    12181 aatagaagca tggcgtttca aggtaatctc atcaggaata tctaatactt cccaatttat
    12241 tttatctgga ttcggaacat gaaaaatgat ttgattctct tcactattcc aataaaagcc
    12301 tgtttggatc tgattcacaa tagcgatttt ttgcgtagaa tagattcgtt tttcaaattg
    12361 gccgaaaaat tgatagactg ccaattcatt cttccatgca gaaaatgcga gaactggaaa
    12421 agaaaggatc gttgtaagta ataaaatcag taagagtgat tcaaacaacg tatatcctga
    12481 tgctaaaacc ttaccttcaa ggccgctgat agatttctgc atgttctcgt ttgatatagc
    12541 cttcattcaa caattcctcc acagtaggaa ctcttccatt attttgaagc gcatagagct
    12601 ctttttgtgt gtctaccaac tggatgatcg cttcacgact ttcttggtct acatgattcc
    12661 tataacgcga caaattaggg acgaacaaca acactaatac actgatgatc agtaagacga
    12721 ttagcatttc gatcaatgta aaaccgctat aataagatat ttttctcttt ttcatggcaa
    12781 aacctcctct atgcttccgt aaacaggtaa taaaacagcg gcataaacta ataaaatcaa
    12841 agccgctacg cccaaaaaaa tgattggctg aataaaatgc atccagtgat gaagacgttt
    12901 gaaaagctgt tttcttgtcc attcgctgta aaaaatcagt tctttaccta aacttccctt
    12961 tgcctctccc tgtaaaacaa ttttactgaa ttcttctgat agaaaacgat aattctggaa
    13021 ctgctcagcc aatgacatac ctgcctccaa tccttgaatc aacttagagg caagatgctg
    13081 aatcagactt tcttgtttcg tttcctttaa gcagactatg atttgcctta attcgaaacc
    13141 ttcatgaaaa agctttccaa tctctagtga caaataagcc gattgataca aggaatatat
    13201 cggtccaatc aataattgtc tgcttatcca ttcactctga tcaacaggag acaacttcat
    13261 cagacgatac cttactagta aagccaatag agtaaccgct aggatgaata gtagaacata
    13321 ccaatgaaag acttgtatca gctgaatacc ccaatgctct tgcatgacca ttcctgaacc
    13381 aataagctga ggcaaaacga acaaccgcat actaaacaag atacctagca aaaaggtgag
    13441 caatagcatc ggatatgcaa gcagcttttt caaatcattg cgaaatcctt caacaagccg
    13501 aaactgtttg gcaatttcca acaaagttgt cggcagattc ccatgacgtt cagctaactc
    13561 gatctgtacc agttgctctg ctccatatcc tatttgcgaa agtacctcac taaacgcttg
    13621 cccttccttt agctgatgtt ggatatttgc caaaataaca ggtggaaacg aacggatcgt
    13681 gagtaataaa taaagggatt cttgcagact aaaaccagat aaaagcagtt ctcccatggt
    13741 ttgtgcaaac tggttttgct ggcttcttgt taatccttta gaatagttct gactgccaaa
    13801 tattttcctg agcttcttca taactttcct cccatgattt ttttactgat tcttcctcca
    13861 taaattgata actccataaa acagaaggat ggtgattggc atcttccaaa agttcttgat
    13921 aaatgacacc agctaaacat tcagatagct cttctttttt ctttgtcagt tcatggattc
    13981 tgaccaatgt accttcaaga ttgcgtgcat gtacggttgc aaaaacccgg tgccctgtta
    14041 gtgctgctct taccgcagca tgtgcagttg attcatctct tatctcgcca atgattagca
    14101 agtctggacg atgccgtaat gcgagtttca aaagtgtatc ataagtctgt gctattttcg
    14161 gattgatctg tagctgcaga aaaaacggtt cttctatctc tacaggatct tcgatagtaa
    14221 tcacttgcaa tttttcttcc aaagccaacc gatacattag agaagtcttg cctgatccga
    14281 ctggtccgct gaaaaggtat aatccacgtc tgacggtttt ctgtaaaata gttgcaaaat
    14341 ctcgagaact aaagcaacga tggggtgttt caaaagtacc gtataatagc cgaatcacta
    14401 aactctctcg ttgaaggtaa tctcctacag aagacaaccg cagacgaact tcctgttttt
    14461 ctaaagaata agtaaatgca cctaactgag cttttctttt ttctccgata tccatctgtc
    14521 ccaaatactt aaatcttgag atcagttgct gtccatctgc cgaagagact ttcttccaga
    14581 atgttcgttt ttttccatag cgaaaataga tgtaaaatac ttctgaagct ggcaatatat
    14641 atacgtcttg ggcatgtttg gcaaaacttt cacttatcat ttgatccgat aatcgtttta
    14701 tttccattat tctcccctcc acaaaaaaca tacgtctttt tgatcttttt gtttcaaata
    14761 aaatgaattt acaaaaaaat attggcaaaa gccgaattta tgttatactt ataaaaattt
    14821 atcttgggag gcactccatt ggaaacaaca ttaaggaaat atatcgaagt agcaagtaaa
    14881 aaaaagcctg ctgacctagt gatcaagcat gctaagatcg tcaacgtctt tacgaaagaa
    14941 atcatggaaa aagatgtagc aatctgcgaa ggaatgatcg taggaatcgg tgaatacgaa
    15001 ggaaaaagcg tacataatgc taatgggcaa tatctagtgc caggattcat cgatggccat
    15061 gtgcatatcg aaagctcact tttatcacca agcgaatttt caaaagtatc cttgcttcat
    15121 ggagttacta ctgtagtgac agatccccat gaaataggaa acattgctgg gagtacaggt
    15181 cttgagttta tgatagaaga tgcaagacaa acactcatga atatcttttt tatgctacct
    15241 tcttgtgtac ctgtaacccc ttttgagacc aatggtgcaa atttaaatgc aaaaaaatta
    15301 gccccttttt tacaaaaacc cgaagtttta ggcttagctg aagtcatgaa ctatcaagca
    15361 gtagcaaata atgaaacaga catcctagaa aagatccaat tgatgcacca gcataagaaa
    15421 aaaattgacg ggcatgcagc tggtatcggc atggaagagt taaatgttta tcctgcagca
    15481 ggtattcgga cggatcatga agctacaact gcaaaagaag ctaaagaacg tttggatctt
    15541 ggtatgtatt taatggttcg agaagggacc gttgcaaaag atctggcatc gctactaccg
    15601 gcgatcacac ctgaaaattc aagaagatgc ttctttgtca ccgatgataa actgatcaat
    15661 gatttagtaa aagaaggatc aatcgatcat atcgtccgtc aagctattgc acttggaatc
    15721 gatccattac aagccattca gatggcaaca ttgaatgcag cagaatgttt tgatttgaaa
    15781 acactaggtg cgattgcccc tgggtatcaa gctgatttct tcttaacaga cgatcttacc
    15841 aagctgccaa ttcatgaagt tttctataag ggaacattag ttgttcaatc tgggcaactc
    15901 caagaccatt tatttgctga tcattcaaat tcatatacgt gttgtctgcc aaaactaaac
    15961 gctcaaccat taaatccaca ttcgttagag ctgcttcttt cttcttcaca agcccatgtg
    16021 atcgaaatta tcccgaatag tctagtgact aaagatttgg tagaaaaagt ggaccgacaa
    16081 ggcgaacgat tcattccttc aatcgaaaaa gaccaattaa agattgcggt gatcgagcgt
    16141 caccatcaaa cgggaaacat tggattaggc atagtgaaag gatttcagct gaaacaagga
    16201 gcgatagcga caacgattgc ccatgactcg cataatctag ttgtcgttgg gacaaacgac
    16261 gaggatatgc tgtatgctgc taatcttttg attcaaaaag gtggcgggat gaccgttgtc
    16321 aacaaacagc aggaaatagc ttgtgtttct cttcctatag gcggaatcat gacacaggaa
    16381 cctttttcga tcgtctatga acagttgaat gaattaataa ctaaagcaca tcaactagga
    16441 gcagctaaaa cctttgatcc ttttttgaca ctttctttct taacattatc tgtcataccc
    16501 gaattgaaga tcaccgacaa aggattattt tcattttctc gcttccaatt gattccacct
    16561 tgtggaaaaa ccgatgaaat aaatacattt taaatcaaat aaatcccaga aaatacatta
    16621 catctgtaat gttcattttc tgggatttat ttatggatat taatgttttt atgaaagcag
    16681 aaagttagtt tcacagactt ttctttttgg tcttatcttg aaattgagct tctactcgat
    16741 aaatacgttg accttttgaa gaaaattttt cttcatactc cgtcataata ttgccttcaa
    16801 attgactttg atgtaaatct aaccatacct gtttgaggat catgccatat tgagaaaaac
    16861 ttgccagaga atactcaaac aatccttgat tatctgtttt gaaatggatt tctccatttg
    16921 gacgaagaat ctgttcatct acagcaagaa aagtcttata tgtcaatcgc cgtttttcat
    16981 gacgtttttt tggccatgga tcggaaaaat tcagatagat ttgatctact tcgctgtcag
    17041 cgaaatactc tgtcaacgct gatccatcca catgtagaag tttcaggtta ggaagcggtt
    17101 gttcgatcaa cttgtctagt gcaatcgaaa caacactgac ctgcatttct actccaatat
    17161 aattgatttc aggatgggct tttgccattc cagtgatgaa ctggcctttc cccataccaa
    17221 tctcaatgtg gattggatga tcgttttcaa acaattcatt ccattttccc ttccacaatg
    17281 ttggatcact aattacaaaa ttcgggtgag ccgctaacat ttccgctgct cccggacgat
    17341 ttcggacacg cattatttat ctccattcct ttcctctaat taaaaaggtc tggccaaacc
    17401 tcagccactg aaaattcgtt ttaattgtaa aatttcttcg ttcatccgtc gatgatcttc
    17461 acgtttgcaa tatctggtga tttcttggag caaattcatg gccgcatacc aatgaaattt
    17521 ttcaagcatt tcgtcagtcg ggcgtatgcc ataactgatc agccactgat tccagctaga
    17581 cagtggcaca taatttccta aaatcgttgc aatatccacc gcagggtctg caaacatgat
    17641 tgaatcccaa tcgactagat ataaataatt tttacatact aaccaattac gatggtttac
    17701 atctccgtgt actactgata agttttcctt atctattgac gggatctgct cacgaagaaa
    17761 acgatggaca atttgcagaa attgattttt ctggagttct tccggtaatt ctttttcata
    17821 tttttgcagc atcttctccg gcgtcatgac acgaccaccg atacgggtca gcatggtttt
    17881 aagagaattt gaatgatgta aatggtacaa aacatcaatc acgtcgtttc tttgaccaat
    17941 ctcattagga gtcagcaagc gaccttccag ccactcttgt gctgtcaaag tatcgccatt
    18001 ccccgttcgt tttgtccaaa caagttttgg tgtaattcct tcccttgaaa gagcagccaa
    18061 catcggagtc gtgtttcgtt taataaagac tttgtcctca tcacgcatgc ctatgtatgt
    18121 ttttcctgtg tcgcctttga tgggacgtaa ccgccactct tggtctaact gaaactccat
    18181 catgcattct cccgtcctct tactctttgt tttgcagtac ttattattct agtcgcctga
    18241 acactacacg tcaagcacat atacgttctt tttcatttaa ttgccattca aaaaaccagc
    18301 ttttctcaaa aaaaatagaa cgaacagcat tcttactgtc aacgctctat ttttcattct
    18361 catttttacc ctacacagct ttgttttcag tctgacatct tcttcaaacg agttggtaca
    18421 tatagtttaa caaagagcat agtaacaatc aaataagccg ctagcggaac ccatcgctgc
    18481 tgcccatcta atacgactgc agcaatcaaa ccaaaaataa ccgacgcgat aattaatacc
    18541 catgtgagta gatactgaat cgcctgcttc ttctgattct tatggattgg ataaagctga
    18601 acgagaacca tatagcggaa ctggttcgct aaaggtaaca gttggaaacc gatcaaatat
    18661 aaaaataaga aaccgataat cagactaaaa taaaagtcga ctactccggc aatcaataac
    18721 ccacctataa ttactaatcg gaaataaaga ccactaaaat cagaacctct tgccaatctt
    18781 cttgcaaaca agtaaagata agtgtttttt gattctcttt taatatgagt caatatagga
    18841 tccaaatagc gtcttctttt gatctttgcc gtaatttcag gaacatctgt gaataaattg
    18901 ataaattgat agatctgatg caacctatgt tcttctacat gcaccatttt ctcccaatcc
    18961 aatccttttt cttgttgctt ccacaaaaac cagtaatata gacttgcctg tactaatgca
    19021 cctgctattc ctgcccatat tagccaataa aatgatagaa ataaaaagat cagagaagaa
    19081 aaaaaccagc ctaatttcca ataacgaata acttgttgat ctagtcgaaa caattctgct
    19141 ctcttgattt gcagatgact gaatttcaaa ctccataata tgatcagata gacgaaaaac
    19201 atcgaaaagg attgctccgt actaaccacc actaatggca tggccaatcc gcatgccaat
    19261 atcaaaaaga tcaacggaaa aatgcaagaa tagcgaaaag ctcgttccaa ataggtttgc
    19321 atttcttttt ctttaggaag taaaaaaaca atatctgctg cctctgcaaa agtagccatc
    19381 ttacccacga ataaacagag catccaaaat acagagatta tgagccctcc aattgcaaac
    19441 ggtggctgca gggttttcaa ccaattagaa taatataatc ccacaccgcc aatcaagaaa
    19501 agacaaacta aaacaaaatg atcgttcaat acataacgca tatatttcat cattttcttt
    19561 tgatgtcttg ctaaacgttt ttgaaaaaaa ctactcatgt gcctgctcct ttgttaaagc
    19621 aatatagatt tcgtccaaag aagaatccgg cagctgaaat tcttttctca acatctcaag
    19681 agaaccgtct gctcgtatct ctccgttatg taagaccaca aaacgatcac aatatttttc
    19741 tgcagtagct aaaatatgcg tagacatcaa aataccggct ccttcttttt tcatttctac
    19801 catcaattca agcaatgcat tgatagctag tggatccaag cctaaaaatg gttcgtcgat
    19861 gatatataaa gaaggctcga tcaaaaaggc gcataagatc atcactttct gtttcattcc
    19921 ctttgaaaaa ttagctggga accagtcaag acgattagat aaccgaaaag tctccaacaa
    19981 cttttctgct cttttcattg ctgtatccat aggtatatcg taggccatcg cagtgacttc
    20041 gatatgttct ctcaatgtca attcttcata taaagaagga gtctctggaa tatacccaat
    20101 ttgtttgcga tattcttccg tatttttctt taaggataga tgattgattt ctatcgttcc
    20161 ttttgaagct tccagcagac cgataatgtg cttgatggtc gtacttttcc ctgctccgtt
    20221 cagtccaatc aaccctacta tctctccagg ctgtacatcg aaactgatat cttttaacac
    20281 agggagatgt ccgtaccccc ctgtgacatg atcgataatt aaactcattc tttttcctcc
    20341 gccatttatt tttatttatt ataccacgaa gaaaatgacg gtattttgtt tttttcctta
    20401 caatagcttt aacttatggt aaagttaagc tgaagtattt attctttata gaaaggatgt
    20461 tttatatgga aaactgtatt ttttgcaaaa tcatcaatca agaaatccca agctataaaa
    20521 tatatgaaga cgacaaggtc tatgcttttt tagacatctc tcaagcaacg aaaggacaca
    20581 cattagttgt gcctaaacgt catgtagcag atatttttga atatgatccc gaacttgcag
    20641 gagaggtttt ttcgcgtgtg cctaagattg ctcaagcatt ggaaaaagct tttccagaaa
    20701 tgaaaggtct taatatcttg aataacaacc gtgaactagc ctatcaatct gtttttcatt
    20761 cacatatcca tttagtccct cgctacagca aagaggatga tttttctatt cactttgtca
    20821 atcatcaaga tagttatgga tcggaagaac taaaagctat tcaagaaaca atcgtaaagc
    20881 aggtgtcctg cgatgattaa aaactttttc aaaggactgt tatttggagc tgctgctggc
    20941 actgtcggag gtctgttatt ggcgcccagg agcgggaatg aaacaagaaa gaaactgatc
    21001 gaagaattgg aagaagcaac aactctgaca aatgatttaa ataacagcct gaatcatttt
    21061 aaaagcgcat tgatcgaaac aaaagaaaca gctgaatctg tgattcctgc tttccaagaa
    21121 gcaatcaaaa aagatatcga agattttaaa ttccaagccg aaccacgtat cacacaaatc
    21181 aatgaacaag tagaaaaact aaaagctgat ctgccagagt atcctgaaca aatataataa
    21241 tgattatttt ctcaacagat ttaactatat aagtgagaaa agtttgcgta tttcctagga
    21301 atgtgatgaa attttgaatt ttgtttgaaa ctaccacgac aacgttttta aaggaaatgg
    21361 gatatggtat agttaatata taaaaataat aactaattac aggagtgcaa attttaatga
    21421 aaaaaaaatc aattattttg gcagctacta gtgcgttggc tgttttaact ctagcagctt
    21481 gttcaggcga tactaataaa gatatcgcaa cgatgaaagg cggaacgatc actgtatctg
    21541 atttctatga tgaagcaaaa cttaaatcat cgaaccaatc cttagtacaa cggatgatca
    21601 tttataaagt attcaataac aaatatggcg ataaagtaac cgataagcaa gtggatgcag
    21661 aatacgacaa acaagcgaaa tcattaggtg atacttttga atcacaatta gaagctgctg
    21721 gatatacgaa agatacttat aaagaatata ttcgtaacaa tttagctttt gaagctggct
    21781 tgaaagctca cgtagatatt acagatgacg atttgaaaac tgcttggaaa tctttccatc
    21841 cagaagtaga agcacaaatc atcaaattat caagtgaaga tgaagcaaaa gatgtgaaaa
    21901 aatcagcgga tgatggagat gatttctcta aattagccaa agacaaatca actgatacag
    21961 agacaaaaga agatggcggt aaagttaaat tcgactcaac gacaacaact attcctgcag
    22021 aagtaaaaga agcagctttc aaattgaaag acggtgagat ctccgatgtt attactacaa
    22081 caaatcctac ttcttatgcg acagaatact acgttgtcaa aatggtgaaa aaccaaaata
    22141 aaggcaatga catggataaa tacaaagatc aactaaaaga tatcgctact gaaactaaac
    22201 taagtgacaa tgcattcaca actaaagtaa tcggtgaaga attaaaagat gcaaatgtaa
    22261 aaatcaaaga tgatgccttt gaaaatgtgt tgagtgcatt cacaacaact tcatcaagta
    22321 cgaaagactc aagtgaaact acagcttcaa caaagagttc tgacacaaaa tcaactgatt
    22381 caacaaaaga atcttcaact gaagaaacaa ctgattcatc aaaataaaag aaaacaaaaa
    22441 aagcgaagac ttatgtctcg ctttttttgt tttgaatgaa aaaaataaat ttttgctata
    22501 aatcttttgg tacatagaaa cttcggttgt ccataccgaa aatccgatcg gtgtattttc
    22561 ctggttctgt ttgacgaatc gaagtcaaca tcatctgcat cgaagcatcg atattgtcga
    22621 tttgatgtaa tatttctgct tccattattc ttggacgtac aggtgagcca tattctaata
    22681 agccatggtg tgctaaaacc atatgacgta atacgagaac atcttcgtca gcttcgctga
    22741 ttttcaacgc aagacaggct ttagttattt cttcgtcaat caataccaga tgaccgatca
    22801 aatttcctgc aagcgtatat tctgtcgaca taggaccgga aagttcaatc acctttccta
    22861 aatcatgcaa aatgatacca gcatacaaca atgctcgatt cagctcagga tattcatcgg
    22921 cgattgattt ccctaagtga agcattgtca aagtatgata agctaatcca ctggcaaatg
    22981 catgatggtt tctttttgct gccggaaact caaaaaattg tttttgatat ttattaagta
    23041 gatagcgaac aatacgattc caatgcgcat tggtgatctc aaacaacaat tgattgattt
    23101 cttcttccat ttcttctttc ttcaaagggg ctctttccat gtataaacta ggctctcctg
    23161 gctcatctac ccttgttaag cgcatatgaa gaatctttac ttgagggttt ccttgataaa
    23221 cctctctttt accgtttaga aaaacgactg tgccggctgt aaatttattg atttcctcct
    23281 ccgaagcatc ccagtattta ccatcgatag tacctgaagt atcttggaaa gtaaaagcaa
    23341 taaatttttt tccgttttta gcgattctta catctgcact tttgatcagg acaaattgtt
    23401 caaactgctc atcgattact aaatcacgaa tttttttcat tatttgctcc ttctaattgt
    23461 tttacgattt ggttcatctc atggtaatag cttaccattt ctttatcaga agaaaaacaa
    23521 attatttgat acttttctcc aaatttagct aaaagttcag ctaattgata ctttcgctga
    23581 tgatcataat gaagccagcc atcatcaatg atgatcgggc atatcgaacg gttagcttct
    23641 aatgagaggt aagcaaatct catcgccata atcagctgat ctttcgtacc tgtagataac
    23701 tcatagatgg gaaattctgt atatccgtct gttaaagtca gtgtatcatt tttcagtaat
    23761 atcttctgat aatttccttt tgttaaaata cctaaataat cacctgctgc tcttaacaac
    23821 tggggtaatt gtctttcact catctctgtt gaaagatcac ctaagaatgt cccggcgatt
    23881 tcaagtgctc cccattcctc cagtaattcc tgtaattttg ctttttgccg gctagcttgt
    23941 tgataaagtt cgtccaatgt gccatctctt tgcaactctt ctacttgaag ttggagtcgt
    24001 tgccgatttt cacttgcttc tttttcttct tgctggagtt gggattgctt ttctttcagt
    24061 tggcgcagtc ttacagataa tttctctttt gtcagctctt caggaaatag tggagtaagc
    24121 atttccgata attcattgta tcttttcatc tgctgttcgg cttgtttggc tttttgtaaa
    24181 aagacaggta cttccgaagg ataagataag cctgcttgag tcaattgttc gctgtattct
    24241 tgtatccata ctttttgttt ctttttcaat tgattgattt cttgcttcaa taaagtattt
    24301 tgctgatagc ttctggcaaa tttcatctgt tccatctctt gtataaaacg ggaaagcaat
    24361 accatttttt ctgaaattgt cttatcttgt atcggtaacc attctttaac aaactcaaat
    24421 ttttggtcga tttgtcgtag cttttcttgt atttcctgtt ctcttttatg tttccgctca
    24481 aatacgccat ataatttctg ataatctatg acttcctgtc catattcttt catgagtttg
    24541 agcgtatcca tctttcctaa gtgccgatga tccgcaaaac gatatacttc ttgctgaaga
    24601 atctgttttt cttctccagt tctcttttgt ttttcttctg cttcagctaa ttgagctaag
    24661 gctagatcaa gttgtcctag tttttcttgc cacattcctt ttatctcaga gaaatcattt
    24721 ttagttttaa tttttggttg ccttttgtag aagaaatagc caatcatgcc tgcaccaatt
    24781 agtaaaagga tcactttcaa gggcattggt acaaatatac ctgcaatgat tattgctccg
    24841 ccagccatca gcatatacgg tttagatgaa gagactggtg ctatctctga ctgattttgt
    24901 aataatgctg gataggtctc ttccaagtca ttcacttctg cttcaatcat ctcttgctgt
    24961 tctttagcta tttggagacg cacagaaatc tggctttcta gatcagcaat ccttctttct
    25021 tcagccagaa gatgcttcca ttcttcttca tataaaaatt cttccggtaa cggatttttc
    25081 tgccaatgcc acctttgttc caaaatcttc aattctgaat ttgtttctct agtctcaatt
    25141 tgtaatcttt gccattcttc gtttaattgt tccgctgtaa attgaagttg ctggagatct
    25201 tttagttcct gctcctgctc taaataaaaa taatatctgg ctgactgctg atccatcccg
    25261 ctatgctttt ccagtgttgc ttctaattct tccaaacgct catttgtttg ctgataccgt
    25321 ttggagaaag actccagatc taaaagaagt tgctcatcag gaagacctgt agtgctgttt
    25381 tttttgagag attgccattc ttcatataaa gaaaaattaa gttcttgctg tctggctaat
    25441 gaaaattgtt tgttcgcctc ttttaattgt gccaataact gctgctgttg cgactcgtat
    25501 tcctgttctt gttggatcaa cttgccaaaa taagcttcct gttcttgttt ttgatgaatt
    25561 ttttcttgca gctctttcca ttcatttaat ttttgattga ttagtagttt ttggcctttt
    25621 ttcttgaata attgttgtgc tttctgataa tattcttgcc gcttttggaa tacttgagaa
    25681 ctgcccgtga tgcctaaaga aatcagcgca tcatgtagct ctttttctct caaatgatct
    25741 agttccatca actgctcttg ctggaaagta aatacctgct ggaataactc tttcgtcaaa
    25801 ggtgcgatca gtttttctag taactcatca cttcccactt gatcccccag ccacaccttc
    25861 gattttccgc gattttgctg cttgtagcgc tcgacagcaa attcaccata tacaggatgt
    25921 ttaagccata ttttgccgcc ataggctgag ccgtcttttg gtgtataatc ctttttttta
    25981 cggccttttg aagggaaacc aaataatatt gcttgaataa attgataaat cgttgatttt
    26041 cctgcttcgt ttcccccata gatcagctga ttgcctgaat aaaagtcaaa acttttttga
    26101 cgccattttc caaaagcagc aatctcgatc cttaaaatct tcattcctga tcctcctcaa
    26161 ataaaaaatc ttgattaagc agctgcttgg ctttttcaag tgtttcttct tgatattccg
    26221 gcagttcgct caatattcta gccgcttctt gatgactata tacttcattt aaaatttgtt
    26281 ggaaatcttg aggtgtctga tagtattgaa ataactgttc catcaattta gcagaagcag
    26341 caagtgggat cttcgtataa aaagtattct ctatgagaga aatacgccaa ataaagaaat
    26401 catttatcct atctgagaat tcctctaaca aatagtctaa tagctctcct gattgtatcc
    26461 gttccaaaac atctgtattg agatggtcat aatcttttaa ttttatctca attaaactaa
    26521 atccatcctc tacttttgaa agctgctgac gaatgtgaac aatgacctct tttgtcgttc
    26581 ttgctttcct caatgatatt tcctttgtct tccaatagac ctctgctact ttaatcggat
    26641 gtatttggca acttccttta cttaattcaa ctagtagaat agatgtagaa gcttgttcct
    26701 ttttagtatg accttgaggc gcacctggat aaacaattgt ttgcttttca tttaagaccg
    26761 ttggcacatg gatatgccct aaagcccaat aatcgtatcc cttttcttgc atctttgagg
    26821 gttggaaagg agcatagttt cctttttgag agacgccagg ttccccgtgg tacaacccaa
    26881 tatggtaatc tgtgagttct ctagaaggaa attccatcac tttgtctttt tgaatccacg
    26941 gttgacgata actaaatcca ctgagagaga cagtttctcc actttttatc gttatttttt
    27001 ttgtctctac catttcggat gtaaataaat ggacattttt aggaaaggag aaccaatacc
    27061 gctctttttg atagaaatca tgatttccga atattataaa tacaggtata tggttctttt
    27121 ctaaccgttt catctgttct gaaaaatgtt tctgtatttt taatgaagga cgattttgat
    27181 gaaaattatc tcctgcaagt aaaacaaaat caacgttatt tattattgcc tgttcaatga
    27241 tatttgataa aacggtcaga ttcgtcttca atagtttttc ctgtacagtt ttatcaagag
    27301 ttgtcagtcc ttcaaatgac cgatccatat gtaaatctgc cgcatggata aatttcacca
    27361 ttaaaaattt cctccttttt ttcttttatc ataccatttt caatgttttt tttcatcaat
    27421 aaccgaaaat ttgttcgtat tatgcaaaga aattacgata aatggccgac ttcaaaaaat
    27481 aagtttattc gaaaaaaaga ttgtgacatg atttttgtca caatcctaaa tatctatgtt
    27541 atattcgctt cgtttgacac tgaagaagta ctatttgtac tggaatttat gctccatttt
    27601 tccgtattta gaattctatg ttctatacta ccttaattag tttatatggt aagaattctt
    27661 tcaaatgctt gagatactcc gtctttatca tttgaaacag tgacataatc agcttttttc
    27721 atgatttctt gatgactatt tcccattgca aaactaatat ctgcactttc aaacatagat
    27781 aagtcattta aactatctcc gatcgcgatt ctcaaaggtt tctttaaatt gtagattgat
    27841 tccaaacgat caaacaccac acctttagat acattttgag gtacgatttc aatatttaca
    27901 ggtgccgatt cagtaacaga cacatttagc atctttttca aatggttaaa tatattagct
    27961 ttcaattgac gattaggaat aaaaataaaa aatttgtaaa tgctgatagc atccttttct
    28021 ataatttcct caatattatt ataagggatt gaacaaatag atttttcata ctcaagctca
    28081 tagtttagct ctttgataga aaattcactc cctacctcgt gaaaaatttt tttcatttta
    28141 tttttataat tctgagattc ataacttccg agatttgtat atattttggt tggaaatttc
    28201 gataggacag aatcaatttt ttttacatca tttttatcga aggaagactc aaatatcttt
    28261 ttttctccat caaatccaac agaaccattt aaccctactg ctggtatcct taaaccagat
    28321 tttttgagaa tattattaat gtcttctact tctcttccag aacaaatgaa tggaaaacag
    28381 tcttccggta aatgtttcat tgttcttgca tttttttgac taatgctctg gtcagaattc
    28441 aaaagagtac cgtctaaatc tataccaatt agtttcattt catccatgtc tcctatacgt
    28501 tgattataaa ttcactgctt attgtaagtc taaatttctc acaaaacata tactaattat
    28561 acctaattta agtaagtaca aacatcattt taagaatagg aaaggacatt tatatactat
    28621 tctctgtatt tttgatttat a
//
LOCUS       DACCSK010000024        28596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCSK010000024 DACCSK010000000
VERSION     DACCSK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.024"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            235..1875
                     /locus_tag="IVP39_000921"
     CDS             235..1875
                     /locus_tag="IVP39_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7586007.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2029..3351
                     /locus_tag="IVP39_000922"
     CDS             2029..3351
                     /locus_tag="IVP39_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7586008.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3413..3844)
                     /locus_tag="IVP39_000923"
     CDS             complement(3413..3844)
                     /locus_tag="IVP39_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7586009.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3908..4744)
                     /locus_tag="IVP39_000924"
     CDS             complement(3908..4744)
                     /locus_tag="IVP39_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7586010.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4934..5236)
                     /locus_tag="IVP39_000925"
     CDS             complement(4934..5236)
                     /locus_tag="IVP39_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7586011.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="IVP39_000926"
     CDS             complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="IVP39_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7586012.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5690..5956)
                     /locus_tag="IVP39_000927"
     CDS             complement(5690..5956)
                     /locus_tag="IVP39_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7586013.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6210..6935)
                     /locus_tag="IVP39_000928"
     CDS             complement(6210..6935)
                     /locus_tag="IVP39_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7586014.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7301..7371)
                     /locus_tag="IVP39_000929"
     tRNA            complement(7301..7371)
                     /locus_tag="IVP39_000929"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7337..7339),aa:Gly,seq:ccc)
     gene            7488..8240
                     /locus_tag="IVP39_000930"
     CDS             7488..8240
                     /locus_tag="IVP39_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7586015.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8256..8735
                     /locus_tag="IVP39_000931"
     CDS             8256..8735
                     /locus_tag="IVP39_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7586016.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8986..10566)
                     /locus_tag="IVP39_000932"
     CDS             complement(8986..10566)
                     /locus_tag="IVP39_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7586017.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10559..11302)
                     /locus_tag="IVP39_000933"
     CDS             complement(10559..11302)
                     /locus_tag="IVP39_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586018.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11478..12254)
                     /locus_tag="IVP39_000934"
     CDS             complement(11478..12254)
                     /locus_tag="IVP39_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586019.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12502..13338)
                     /locus_tag="IVP39_000935"
     CDS             complement(12502..13338)
                     /locus_tag="IVP39_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7586020.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13349..14278)
                     /locus_tag="IVP39_000936"
     CDS             complement(13349..14278)
                     /locus_tag="IVP39_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7586021.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="IVP39_000937"
     CDS             14470..14823
                     /locus_tag="IVP39_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7586022.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="IVP39_000938"
     CDS             complement(14897..15553)
                     /locus_tag="IVP39_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7586023.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15779..17710
                     /locus_tag="IVP39_000939"
     CDS             15779..17710
                     /locus_tag="IVP39_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7586024.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEIAELLAQDEPESAPAPTNKSDYYQSKEQ
                     QKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQDQ
                     ETKLTEWEELSLELEEYEN"
     gene            complement(17796..19019)
                     /locus_tag="IVP39_000940"
     CDS             complement(17796..19019)
                     /locus_tag="IVP39_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7586025.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19013..20911)
                     /locus_tag="IVP39_000941"
     CDS             complement(19013..20911)
                     /locus_tag="IVP39_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7586026.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20905..22023)
                     /locus_tag="IVP39_000942"
     CDS             complement(20905..22023)
                     /locus_tag="IVP39_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7586027.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22106..22939)
                     /locus_tag="IVP39_000943"
     CDS             complement(22106..22939)
                     /locus_tag="IVP39_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7586028.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22932..23849)
                     /locus_tag="IVP39_000944"
     CDS             complement(22932..23849)
                     /locus_tag="IVP39_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7586029.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23872..24051)
                     /locus_tag="IVP39_000945"
     CDS             complement(23872..24051)
                     /locus_tag="IVP39_000945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586030.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24076..25200)
                     /locus_tag="IVP39_000946"
     CDS             complement(24076..25200)
                     /locus_tag="IVP39_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7586031.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTTTVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25383..26291
                     /locus_tag="IVP39_000947"
     CDS             25383..26291
                     /locus_tag="IVP39_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7586032.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26432..28045
                     /locus_tag="IVP39_000948"
     CDS             26432..28045
                     /locus_tag="IVP39_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7586033.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8889 a         5619 c         4867 g         9221 t
ORIGIN      
        1 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
       61 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
      121 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
      181 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
      241 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      301 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
      361 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
      421 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
      481 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      541 aacgaaattt tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat
      601 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
      661 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
      721 ggtaaattga tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta
      781 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
      841 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
      901 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
      961 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
     1021 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
     1081 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
     1141 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1201 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
     1261 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
     1321 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
     1381 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
     1441 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
     1501 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
     1561 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
     1621 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1681 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1741 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
     1801 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
     1861 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1921 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
     1981 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     2041 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
     2101 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
     2161 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
     2221 ttatttcatt ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc
     2281 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
     2341 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
     2401 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2461 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
     2521 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
     2581 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
     2641 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
     2701 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
     2761 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
     2821 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
     2881 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
     2941 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
     3001 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
     3061 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
     3121 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
     3181 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
     3241 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
     3301 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3361 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
     3421 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
     3481 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
     3541 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
     3601 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
     3661 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
     3721 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
     3781 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
     3841 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
     3901 ccttttttca cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt
     3961 ctaatgtttg caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa
     4021 tcgcttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4081 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4141 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     4201 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     4261 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     4321 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     4381 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     4441 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     4501 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     4561 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     4621 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     4681 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     4741 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     4801 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
     4861 gtgctaaacg aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat
     4921 cgattctttt gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat
     4981 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
     5041 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa
     5101 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg
     5161 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat
     5221 ggttatgttc tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct
     5281 gagccgtcta aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt
     5341 cgtttcgctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
     5401 agtcgttcat cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat
     5461 gacatggaag cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact
     5521 acaaaacggt ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct
     5581 tcttctgcct cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg
     5641 ctcacagcaa cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca
     5701 ctccatgatt tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac
     5761 ggcgaatcaa atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca
     5821 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
     5881 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
     5941 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
     6001 acacccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat
     6061 aaattaagaa acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct
     6121 ctttccataa agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata
     6181 aagttcataa aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc
     6241 tcataattga tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
     6301 ttgtcttcaa tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg
     6361 aaatgaggag tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata
     6421 tcattttcgt gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt
     6481 ttaatgttat tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac
     6541 cattgtccgt cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct
     6601 ctcttttcag taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc
     6661 aatttgtcta tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt
     6721 gtaaactccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
     6781 acatccgcta cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag
     6841 atatctgaat ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg
     6901 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc
     6961 cctaaatctt aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa
     7021 aaaataaaac cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct
     7081 ctactcaatc acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta
     7141 atactcaaaa ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca
     7201 tctgcggtaa taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct
     7261 tattacttga tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa
     7321 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa
     7381 acagtatacg cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg
     7441 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     7501 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     7561 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     7621 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     7681 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     7741 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     7801 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     7861 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     7921 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     7981 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     8041 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     8101 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     8161 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     8221 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     8281 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     8341 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     8401 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     8461 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     8521 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     8581 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     8641 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     8701 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     8761 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     8821 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     8881 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     8941 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     9001 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     9061 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     9121 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     9181 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     9241 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     9301 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     9361 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     9421 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     9481 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     9541 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     9601 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     9661 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     9721 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     9781 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     9841 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     9901 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     9961 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
    10021 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
    10081 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
    10141 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
    10201 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
    10261 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
    10321 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
    10381 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
    10441 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
    10501 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
    10561 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
    10621 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
    10681 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
    10741 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
    10801 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
    10861 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
    10921 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
    10981 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
    11041 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
    11101 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
    11161 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
    11221 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
    11281 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
    11341 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
    11401 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
    11461 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
    11521 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
    11581 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
    11641 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
    11701 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
    11761 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
    11821 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
    11881 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
    11941 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
    12001 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
    12061 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
    12121 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
    12181 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
    12241 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
    12301 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
    12361 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
    12421 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
    12481 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
    12541 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
    12601 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
    12661 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
    12721 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
    12781 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
    12841 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
    12901 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
    12961 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
    13021 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
    13081 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
    13141 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
    13201 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
    13261 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
    13321 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
    13381 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
    13441 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
    13501 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
    13561 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
    13621 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
    13681 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
    13741 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
    13801 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
    13861 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
    13921 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
    13981 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
    14041 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
    14101 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
    14161 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
    14221 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
    14281 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    15721 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    15781 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    15841 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    15901 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    15961 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    16021 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    16081 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    16141 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    16201 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    16261 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    16321 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    16381 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    16441 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    16501 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    16561 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    16621 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    16681 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    16741 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    16801 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    16861 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    16921 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    16981 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    17041 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    17101 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    17161 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    17221 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    17281 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    17341 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aaatcgccga
    17401 attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta
    17461 ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga
    17521 agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc
    17581 agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag
    17641 acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata
    17701 tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt
    17761 tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta
    17821 gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc
    17881 ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt
    17941 gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc
    18001 ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc
    18061 accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa
    18121 caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca
    18181 aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct
    18241 tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt
    18301 tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa
    18361 taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt
    18421 tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct
    18481 tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa
    18541 cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt
    18601 attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg
    18661 ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga
    18721 aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct
    18781 gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat
    18841 tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg
    18901 atctcattat tccattcttt ttccattaat tctttataac attcatttat ccatttttct
    18961 ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata
    19021 attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat
    19081 caattttggg acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca
    19141 ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag
    19201 tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt
    19261 taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc accctatagt
    19321 tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc
    19381 ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc
    19441 cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg
    19501 caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat
    19561 aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc
    19621 ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac
    19681 caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg
    19741 gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat
    19801 tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc
    19861 aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag
    19921 ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca
    19981 ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta
    20041 accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa
    20101 agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt
    20161 ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc
    20221 taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag
    20281 gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac
    20341 aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt
    20401 tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat
    20461 attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt
    20521 tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac
    20581 gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt
    20641 ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg
    20701 ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc
    20761 cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat
    20821 ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt
    20881 tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag
    20941 taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct
    21001 atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata
    21061 gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac
    21121 agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt
    21181 atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat
    21241 tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt
    21301 gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga
    21361 attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa
    21421 ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc
    21481 atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag
    21541 taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc
    21601 tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc
    21661 tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa
    21721 taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg
    21781 ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca
    21841 taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc
    21901 tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc
    21961 atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt
    22021 catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact
    22081 agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa
    22141 atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta
    22201 gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa
    22261 ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat
    22321 tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta
    22381 cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca
    22441 gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa
    22501 tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa
    22561 ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca
    22621 acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg
    22681 ctgtagctga aactgtactg attacagcaa taacaatcga attccaaata atcaagggat
    22741 aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat
    22801 tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat
    22861 atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt
    22921 gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata
    22981 acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca
    23041 aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa
    23101 gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact
    23161 gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat
    23221 ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca
    23281 gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat
    23341 attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa
    23401 aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc
    23461 gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt
    23521 gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct
    23581 gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct
    23641 agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca
    23701 aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc
    23761 aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt
    23821 gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt tttaatcacc
    23881 agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc
    23941 aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga
    24001 ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca atagttcata
    24061 gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa
    24121 tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag
    24181 cataaatgta agaaattgtt gtgccacttg tggatttttg gcatctttag ggacataaaa
    24241 acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg
    24301 agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca
    24361 gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta
    24421 gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc
    24481 tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag
    24541 catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt
    24601 tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa
    24661 atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc
    24721 actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg
    24781 atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc
    24841 atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg
    24901 catatcttcg tgtgttacca attttggggt gatcatttgt actgttgttg ttcccgaagc
    24961 tacattcaca atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc
    25021 attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt
    25081 ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact
    25141 taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat
    25201 gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat gtatattaac
    25261 ggaattagta acacaaatat ttatataaaa aaatatttta cttgtcgttt tggcttttca
    25321 tttttgttat aatagtcatt aattataaat agaaagcttt ttatataaaa agagaaagga
    25381 aagtggttct tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa acactaacct
    25441 attccataga tacccatctt catttatctg ctgctcccta ttttcgacta ctaataaatg
    25501 aaagcgctaa tttatatgct gaatttgcac ataaatctat agaactagat aatccttccc
    25561 ttttattcat tgataaacag caagaagtcc aaatcttttc tactcaaaag gaaacacaaa
    25621 cttttatcat tatccagttt tattttgatg agcaattatt agcacatatt acgggacaat
    25681 ctttttctat agatcaagaa ataacatcta tcattacttt tctgaaaaat gaacagttca
    25741 aaattgatta tctaaaacat agtaagccag ctatttccta tgatcaggaa atcaattctt
    25801 ttttcatact atgccaacag atgagcattc atttgctttc cttattagtt acttattgtc
    25861 tgcaaaaaat tcaattgtct tccttaccaa ggcattatca acaggaactt gctttattga
    25921 caataccttc aaatgagaaa gtcattccta aaaaaaagca tattctttat tcagatgaat
    25981 tagttgatcc aatcatacga tatttagaag aacatctttc agaaaatttt ttgcttgaac
    26041 aattagccca aatttttttt atcagtccag ccagcttaaa aaaacatttt agaaaagtga
    26101 atggaaaaag tgttattagc tattttaaag aactaaaaat ggaaaaagca aaacgtctcg
    26161 tgctagaaaa tgaattaacc tatacagaaa ttgcagatat attaggattt cattcgatcc
    26221 atcatttttc ttcagctttt aaaaaatata cagggttatc cccttcaaag tattatctat
    26281 ccttaaaata gaaaaaaatt tttaggtttt atccacaaat tatttctcta tattttctgt
    26341 ctattcaaaa taaacaataa ttttccggca caattatttg ctgtttttta ccataacata
    26401 gtacgatcaa cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc
    26461 tggcggtcct tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg aaatcattat
    26521 ctatgaacgc ggagaaaata tttcttatgc aagttgtgct ttgccttatt atttaggagg
    26581 tgtgatcact gaccgtgact cgctgatcga acgtacacca gaaatattga aaacaaaaaa
    26641 caacatcgat gtctttacta aacacgaagt aacagcaatc gatccttcta ctaagcgatt
    26701 aacagttaaa gacctatcca caaatgaaga aataaaaaca gattacgata agttgatcat
    26761 ctcttctggt gctagaccag attatccgga tattcccgga gtttttgaag cagaaaacgg
    26821 ctttgtcctc cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa
    26881 tccacaacat gtcgtcattc ttggtgcagg tgttatgggt ctggaattag ctgagaattt
    26941 caagcatcgc ggcttagacg tgactttaat cgatcaattg ccacaagtcg ctttccctta
    27001 tgatccagaa attgctaatt tagtttatga caaattgctg aaagaaggat tagccgttca
    27061 tttagaaaca agagttactg agatccgtga taaaggtcga gaaatagtat tatcagatgg
    27121 ctccgtcctt tctgctgata tgctaatttt tgctgttggt gtttctccga agaatgaagt
    27181 ggtgaaagca accggcatac aattatcaga tacaggtcag atcattgttg atgaccagtt
    27241 acaaaccaat ctgccggaca tctatgcgat tggcgatatt atcgaaacga ctagtgtagt
    27301 gactggtcag ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag gacatatgct
    27361 ggcagatatt ttaaatggta cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc
    27421 aaaaatcttt gatcatacag caagttatgc gggaatgaca gaacatgaat taaaagcatc
    27481 aggcataaca aattataaaa ctgtttttat cactcccttt gaccatgcct atttctatcc
    27541 agaagctaca agattaaatc taaagctgat ttttgatgca gatagcggtc gtattttagg
    27601 tggacaagca tttggagaaa aaggtgtcga taaacggatg ggagaacttt ctgtagcaat
    27661 caccggaaac ttgacagtct ttgatttgcc tgatttggag ttgccttact ctccaccgta
    27721 ttctactacc cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa
    27781 tatcgtagaa acgatcaaag caagtgatat acccgaaaac gatttgaaag aagcgttctt
    27841 tttagatata cgtgaaccta ataaagcacc ttctggcagt atctcggcaa caaaaaatat
    27901 tccaatgaat gagttacgag atcgaatcaa tgaaatccca aaagataaaa aagtttacat
    27961 tactttcaga agaggattga atacttatac ttctgcccga atcttggcag gtttgggtat
    28021 caaagcgatt ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag
    28081 ttatttttac ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata
    28141 tagaatagaa gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata
    28201 gtttaagaaa gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa
    28261 aacatgttgt gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc
    28321 ctaatattac gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt
    28381 cgaatatttt ttcttatata ataaagtttg ctttcactcc tttaatctca ccaaatcgac
    28441 tggcttgcgt tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc
    28501 atatttttcg ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta
    28561 ttaaataatt taattttttt gttcctattt atttaa
//
LOCUS       DACCSK010000025        28188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCSK010000025 DACCSK010000000
VERSION     DACCSK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.025"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(130..825)
                     /locus_tag="IVP39_000949"
     CDS             complement(130..825)
                     /locus_tag="IVP39_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7586034.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IVP39_000950"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IVP39_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7586035.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IVP39_000951"
     CDS             complement(2024..2215)
                     /locus_tag="IVP39_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7586036.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IVP39_000952"
     CDS             complement(2236..3126)
                     /locus_tag="IVP39_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7586037.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IVP39_000953"
     CDS             complement(3113..3880)
                     /locus_tag="IVP39_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7586038.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IVP39_000954"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IVP39_000954"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7586039.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IVP39_000955"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IVP39_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7586040.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IVP39_000956"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IVP39_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7586041.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IVP39_000957"
     CDS             8496..8834
                     /locus_tag="IVP39_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586042.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IVP39_000958"
     CDS             complement(9002..9760)
                     /locus_tag="IVP39_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7586043.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IVP39_000959"
     CDS             complement(9778..10596)
                     /locus_tag="IVP39_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7586044.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IVP39_000960"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IVP39_000960"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7586045.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IVP39_000961"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IVP39_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7586046.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IVP39_000962"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IVP39_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7586047.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="IVP39_000963"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="IVP39_000963"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7586048.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="IVP39_000964"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="IVP39_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7586049.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="IVP39_000965"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="IVP39_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7586050.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="IVP39_000966"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="IVP39_000966"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7586051.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="IVP39_000967"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="IVP39_000967"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7586052.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="IVP39_000968"
     CDS             20638..20937
                     /locus_tag="IVP39_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7586053.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="IVP39_000969"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="IVP39_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7586054.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="IVP39_000970"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="IVP39_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7586055.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="IVP39_000971"
     CDS             21904..23877
                     /locus_tag="IVP39_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7586056.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="IVP39_000972"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="IVP39_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7586057.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="IVP39_000973"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="IVP39_000973"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7586058.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26756..28048
                     /locus_tag="IVP39_000974"
     CDS             26756..28048
                     /locus_tag="IVP39_000974"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7586059.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8588 a         5525 c         5600 g         8475 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact
    18181 atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa
    19021 gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa
    19681 taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattggat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacgtcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
    26221 gctgatctta cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag
    26281 tcacaatatc atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt
    26341 aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
    26401 acgcccaatc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
    26461 tgcaggacag ctttttcaca acctctgatt cacggtgttc caaaagtagc ttcttcgaaa
    26521 aataagtcga aatttcacaa aaatttgaga agcaattttc gtacattcct tcttatttct
    26581 tgaagctgac cacttctgtt acaacctctt ttttaataac cattctaaaa agataaagtt
    26641 cgttttttaa gcgtttttat aaaataaaaa taaaaatcat tcgattttat ttgaaatatg
    26701 tatctattat tgatacaatg gttcaggatt aaataatata gaacgaggtg ttcacatgtc
    26761 atctgtagta gtagtaggta cgcaatgggg cgatgaaggg aaaggaaaaa tcacagactt
    26821 tctcagtgaa aatgctgaag tgattgcacg gtaccaaggt ggcgacaatg cagggcacac
    26881 aattaagttt aacggtgtga cttataaact gcatttgatt ccttctggga ttttttataa
    26941 agacaagatc agtgtgatcg gaaacggcgt tgtagtcaat cctaaatcat tggttaaaga
    27001 attagcttat ttacatgaaa aaggtgtaga aacaagtaat ttgcgtattt ctgaccgcgc
    27061 gcacgtaatc ttgccttatc atatcaaatt ggaccaatta caagaagatg ctaagggcga
    27121 aaataagatt ggtacaacga ttaaaggaat cggacctgcc tatatggata aagcagctcg
    27181 tgtaggtatc cgtattgcag acttattaga taaagaaatt tttgaagaac gtcttcgttt
    27241 gaatctagat gacaaaaacc gtcaatttgt aaaaatgttt gattcagaac cacttgattt
    27301 tgacgatatt tttgaagaat attatgaata cggacaacaa atcaaacaat acgtcacaga
    27361 tacttccgtt atcttgaatg atgctttgga tgcaggcaaa cgcgtgttgt ttgaaggcgc
    27421 acaaggtgta atgctggata tcgaccaggg aacttatcct tttgttactt cttctaaccc
    27481 agtagccggt ggggtaacaa tcggaagcgg tgtagggcct tcaaaaatca ataaagttgt
    27541 cggcgtctgc aaagcctata cgtcacgtgt cggtgatgga ccatttccaa ctgaattatt
    27601 tgatgaaaca ggagaacaaa tccgcgaagt aggacgagaa tacggtacga caactggtcg
    27661 tccgcgtcgt gtaggctggt ttgattctgt agttatgcgc cattctaaac gagtttcagg
    27721 tattaccaac ctatctttaa actcgatcga tgtattaagt ggattggaaa cagtaaaaat
    27781 ttgtacggct tatgaattag atggcgaact aatctaccac tatccagcaa gcttgaaaga
    27841 gttgaaccgc tgcaaaccaa tctatgaaga attgccaggc tggtcagaag acattacagg
    27901 atgcaaaaca cttgcagaac tgccagaaaa cgcacgtaac tatgtacgtc gtatctcaga
    27961 gcttgttggt gtacgtatct caacattttc tgtcggacct gatagaacac aaacaaacat
    28021 cttagaaagc gtctgggcgc aaatctaagg ataaaaacga cgactgaatc aggacgttct
    28081 taccagagcg agtcgaagtg tttatctata cgataataat aacttgaaca ctgtttattc
    28141 agaataaggg actgtgacaa aaaccgtgtc acagtccctt tttccgaa
//
LOCUS       DACCSK010000026        28135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCSK010000026 DACCSK010000000
VERSION     DACCSK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.026"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(135..2288)
                     /locus_tag="IVP39_000975"
     CDS             complement(135..2288)
                     /locus_tag="IVP39_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7586060.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2582..3430)
                     /locus_tag="IVP39_000976"
     CDS             complement(2582..3430)
                     /locus_tag="IVP39_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7586061.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="IVP39_000977"
     CDS             complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="IVP39_000977"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7586062.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5931..6110)
                     /locus_tag="IVP39_000978"
     CDS             complement(5931..6110)
                     /locus_tag="IVP39_000978"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7586063.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="IVP39_000979"
     CDS             complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="IVP39_000979"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7586064.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8258..10537
                     /locus_tag="IVP39_000980"
     CDS             8258..10537
                     /locus_tag="IVP39_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7586065.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10630..11277)
                     /locus_tag="IVP39_000981"
     CDS             complement(10630..11277)
                     /locus_tag="IVP39_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586066.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11456..12175)
                     /locus_tag="IVP39_000982"
     CDS             complement(11456..12175)
                     /locus_tag="IVP39_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7586067.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12306..13457)
                     /locus_tag="IVP39_000983"
     CDS             complement(12306..13457)
                     /locus_tag="IVP39_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7586068.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13674..14414)
                     /locus_tag="IVP39_000984"
     CDS             complement(13674..14414)
                     /locus_tag="IVP39_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7586069.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="IVP39_000985"
     CDS             complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="IVP39_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7586070.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14798..15391)
                     /locus_tag="IVP39_000986"
     CDS             complement(14798..15391)
                     /locus_tag="IVP39_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7586071.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15375..16022)
                     /locus_tag="IVP39_000987"
     CDS             complement(15375..16022)
                     /locus_tag="IVP39_000987"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7586072.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="IVP39_000988"
     CDS             complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="IVP39_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7586073.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="IVP39_000989"
     CDS             complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="IVP39_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7586074.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17468..17995)
                     /locus_tag="IVP39_000990"
     CDS             complement(17468..17995)
                     /locus_tag="IVP39_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7586075.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18175..18960)
                     /locus_tag="IVP39_000991"
     CDS             complement(18175..18960)
                     /locus_tag="IVP39_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7586076.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18953..19822)
                     /locus_tag="IVP39_000992"
     CDS             complement(18953..19822)
                     /locus_tag="IVP39_000992"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7586077.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19823..21202)
                     /locus_tag="IVP39_000993"
     CDS             complement(19823..21202)
                     /locus_tag="IVP39_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7586078.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="IVP39_000994"
     CDS             complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="IVP39_000994"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7586079.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21654..22130)
                     /gene="accB"
                     /locus_tag="IVP39_000995"
     CDS             complement(21654..22130)
                     /gene="accB"
                     /locus_tag="IVP39_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7586080.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="IVP39_000996"
     CDS             complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="IVP39_000996"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7586081.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="IVP39_000997"
     CDS             complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="IVP39_000997"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7586082.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="IVP39_000998"
     CDS             complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="IVP39_000998"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7586083.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25160..25384)
                     /locus_tag="IVP39_000999"
     CDS             complement(25160..25384)
                     /locus_tag="IVP39_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7586084.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25437..26399)
                     /locus_tag="IVP39_001000"
     CDS             complement(25437..26399)
                     /locus_tag="IVP39_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7586085.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26409..26849)
                     /locus_tag="IVP39_001001"
     CDS             complement(26409..26849)
                     /locus_tag="IVP39_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7586086.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27155..28120
                     /locus_tag="IVP39_001002"
     CDS             27155..28120
                     /locus_tag="IVP39_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7586087.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         7781 a         6186 c         4877 g         9291 t
ORIGIN      
        1 ttttttgccg agtaaacggt gttcaaaagc cagtttctcg gtatgaagtc aaatttaagc
       61 aaaaatgtga gaagctattt tcacaaaatt tttcttcata ctcgaaacta agcggctttt
      121 tcacgacctc ttttttattt tgccatgaac atatcataat acgcgtccat tatttcttta
      181 gcaatcgtca agttcatgtg gtcttgttcg tctaataagt tcggcaagac aacactgatg
      241 gcaatttccg gattgtctgt tggtccgtat gccacgatat tactattgac tgtcgtaata
      301 tctggatgac cttccgcaac cgtttctgca gtccctgttt ttgcggaaag gtccattttc
      361 gcactggcta atggtttggc tgctgtgtaa ggatcggtac catggacaac ttggtggaat
      421 ccttcacgga ggatcgccat attttctgca gaaatattga ccgtattttc tactgttgtg
      481 ccaatttctt tcttcacttc acccaagttt ccattttcgt cattgccata gatcgctttg
      541 actaggtgcg gctgtactcg tttcccgcca ttagcgactg ttgaagcata ttgagctaat
      601 tggattggtg tatatgtgtc aaactgtcca aatgccaaat cggtaaagtt acctggagta
      661 taccattctt tgccattatc cgcattgaac ttcttcatat aatcaacaga acgtgagatc
      721 ccaggagatt cattcggcaa atcgatccca gtcgttgtac caagaccaaa ctctttgaaa
      781 gagtctctca attgctgata agctttagct tcttcatcta aactaggtag tcccattcca
      841 ggtgtatagt ctagtcctaa catctttaac gccactttga tcatatacgt attagaagac
      901 aattccaatg ctttgactgc atcaagagca acttgtccgc ttcgattaaa gacagagctt
      961 ttttctgaag caccttgcag acgaatcggc tcatcaatca aaacttgatt tccgctgatc
     1021 gcgttacttg cccagcctgc tgtcaaagta ccagccttga ctacagaccc tggagcaaag
     1081 gcgctagtaa tcgtgcctaa agcattctcg gtgatttctt tggaaccttg ttcatgactg
     1141 aaaccagtca ttgctagcac ttctcctgtc tgaggattca tagccactgc ataagcacca
     1201 ggcgaatact gtgctttgcc gttgttgatc aatgtttgat aattcctttt caaaatttct
     1261 tctacttttg attggaattc tgcattcata gataacatta aattagaacc tttttcccca
     1321 gagaagattt ctttttgatt cgaaacattt ccttcattat caagggaaac ttcatattgg
     1381 gttttggttc cttgcaggac atcttcatat tgtttttcca aataactttg tcctacacgg
     1441 tcattccgag aataaccttt tgctaggtat ttctctgctt catctgctgg aagtccttgc
     1501 ttttcagtcg tcacactgcc aagaatactt ttcagtgaac ttgctgcatt atattctcgt
     1561 gtccagtctg tgccagtcga aactcctgga agttcagaag cgcgttctgc cacaacagct
     1621 aactcttgat cagtaacacc agagtttttg atatagacag tgtttaattc atatgcacta
     1681 ttcatccgtt taaagatggt agcaattttt aattgctcct cgtttaaatt aatctcatca
     1741 gctgtgactt tattgaccat cgcactatat tgttcgctgt tccctaattg cttttctttt
     1801 gccgaaagtc tttttgtggc ttctttcaaa tgtttgggat cagccagcca aaaatctttt
     1861 ttatctctat ctgtcagatt ttcatctacc ggaacatcga tcagcgagtt cagcttcgtt
     1921 gcgatttcaa gcaaatcttt agccgtaact tgtgttcctc tcgtatatgt gatggcctga
     1981 ttcgctttat tcgtaactaa agcttttcct gtggagtcgt agatcattcc gcgaggagta
     2041 ctttcttgga ccgtaatcgt agaagtagac ttcactcgcg cctccatctc ttcgccgttc
     2101 acgatctgca aataacccaa tctagcaatc aacgcaacaa ataacgtaaa tataatgaaa
     2161 aaaagaaaat ttaagcgaaa aggaacatgg gaccggcgaa acgttttgtt cttaggttct
     2221 tgtgcacctt ttcttatttt ttctaaccaa gaagtattta atattttttt cataaaatct
     2281 ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc caaaaacttc
     2341 ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc aagataaaat
     2401 caactttttg tttcacagca atcacttaaa gttacatatc caactatttt tttcattaaa
     2461 aaataaactt atttttgaaa attatgtttt cttaagtcgt gaaacacttt gatggatcat
     2521 ttatttgtgg aaaactacgt tttgttattc taaaaaactt atataaaaaa tagtaaggtt
     2581 tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc
     2641 taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg
     2701 ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta
     2761 cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt
     2821 cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt
     2881 ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt
     2941 ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt
     3001 ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac
     3061 actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta
     3121 cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat
     3181 cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg
     3241 tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg
     3301 aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt
     3361 agttggccaa ctttttttct gctgaagaat aatcttccat catactttca atacgactaa
     3421 ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga
     3481 cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat
     3541 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
     3601 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
     3661 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
     3721 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
     3781 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
     3841 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
     3901 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
     3961 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
     4021 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
     4081 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
     4141 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
     4201 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
     4261 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
     4321 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
     4381 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
     4441 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
     4501 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
     4561 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
     4621 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
     4681 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
     4741 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
     4801 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
     4861 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
     4921 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
     4981 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
     5041 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
     5101 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
     5161 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
     5221 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
     5281 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
     5341 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
     5401 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
     5461 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
     5521 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
     5581 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
     5641 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
     5701 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
     5761 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
     5821 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
     5881 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
     5941 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
     6001 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
     6061 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
     6121 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
     6181 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
     6241 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
     6301 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
     6361 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
     6421 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
     6481 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
     6541 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
     6601 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
     6661 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
     6721 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
     6781 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
     6841 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
     6901 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
     6961 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
     7021 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
     7081 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
     7141 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
     7201 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
     7261 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
     7321 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
     7381 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
     7441 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
     7501 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
     7561 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
     7621 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
     7681 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
     7741 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
     7801 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
     7861 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
     7921 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
     7981 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
     8041 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
     8101 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
     8161 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
     8221 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
     8281 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
     8341 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
     8401 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
     8461 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
     8521 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
     8581 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
     8641 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
     8701 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
     8761 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
     8821 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
     8881 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
     8941 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
     9001 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
     9061 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
     9121 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
     9181 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
     9241 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
     9301 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
     9361 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
     9421 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
     9481 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
     9541 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
     9601 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
     9661 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
     9721 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
     9781 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
     9841 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
     9901 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
     9961 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
    10021 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
    10081 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
    10141 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
    10201 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
    10261 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
    10321 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
    10381 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
    10441 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
    10501 tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt
    10561 aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt
    10621 taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag
    10681 cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct
    10741 atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg
    10801 gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg
    10861 aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct
    10921 ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc
    10981 ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag
    11041 cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa
    11101 ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa
    11161 tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac
    11221 tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt
    11281 cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat gctctcatca
    11341 taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag
    11401 tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt
    11461 cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa
    11521 tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca
    11581 tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac
    11641 tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc
    11701 gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa
    11761 aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc
    11821 cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca
    11881 atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt
    11941 atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta
    12001 cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag
    12061 gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat
    12121 tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt
    12181 cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg
    12241 tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca
    12301 attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat
    12361 tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt
    12421 cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat
    12481 ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc
    12541 agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc
    12601 tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct
    12661 attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga
    12721 taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct
    12781 tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg
    12841 gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta
    12901 cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt
    12961 aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga
    13021 aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc
    13081 tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa
    13141 gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact
    13201 gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact
    13261 aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt
    13321 tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt
    13381 tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata
    13441 atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac
    13501 aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac
    13561 acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata
    13621 taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct
    13681 ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca
    13741 gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat
    13801 ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt
    13861 tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta
    13921 tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca
    13981 tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga
    14041 tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca
    14101 gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc
    14161 acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct
    14221 tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct
    14281 ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa
    14341 aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat
    14401 gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac
    14461 catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca
    14521 tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca
    14581 tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg
    14641 ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa
    14701 gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct
    14761 ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat
    14821 tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg
    14881 tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat
    14941 cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt
    15001 caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg
    15061 catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg
    15121 cattattata attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact
    15181 cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag
    15241 gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct
    15301 tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag
    15361 cagtatattc attactatag tccatattca tacaaccctt cattttgaat ataatcaatt
    15421 actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc
    15481 gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt
    15541 gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta
    15601 taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc
    15661 ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct
    15721 aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg
    15781 agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc
    15841 atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt
    15901 gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag
    15961 aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc
    16021 atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt
    16081 gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt
    16141 tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat
    16201 actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg
    16261 atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa
    16321 cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt
    16381 ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg
    16441 atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga
    16501 acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca
    16561 tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt
    16621 gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg
    16681 cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac
    16741 ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata
    16801 attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag
    16861 gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg
    16921 atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt
    16981 ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca
    17041 ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat
    17101 tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa
    17161 agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca
    17221 aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc
    17281 aaaaagtcgt catccgtcaa tgagacatct tggatctcat tataatggcg taatctgaaa
    17341 caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg
    17401 tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat
    17461 tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg
    17521 ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa
    17581 tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa
    17641 tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt
    17701 tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa
    17761 tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat
    17821 ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa
    17881 tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag
    17941 gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt
    18001 ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg
    18061 catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta
    18121 caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata
    18181 ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc
    18241 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    18301 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    18361 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    18421 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    18481 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    18541 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    18601 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg
    18661 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    18721 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    18781 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    18841 aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    18901 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    18961 gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt
    19021 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    19081 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    19141 tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc
    19201 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    19261 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    19321 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    19381 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    19441 tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat
    19501 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    19561 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    19621 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    19681 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    19741 caggaacaga tggcgagtta gcaggtttgc tctctctatt agggttgatc cgaatatatt
    19801 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    19861 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac
    19921 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    19981 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    20041 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc
    20101 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    20161 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    20221 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    20281 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    20341 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    20401 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    20461 agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat
    20521 ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat
    20581 ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    20641 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    20701 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    20761 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    20821 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    20881 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    20941 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    21001 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    21061 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    21121 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    21181 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    21241 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    21301 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    21361 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    21421 gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct
    21481 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    21541 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    21601 atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt
    21661 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    21721 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    21781 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    21841 ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    21901 actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc agatatgaca
    21961 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    22021 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    22081 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    22141 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg
    22201 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    22261 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    22321 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    22381 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    22441 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    22501 caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag
    22561 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt
    22621 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac
    22681 ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    22741 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    22801 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    22861 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    22921 ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    22981 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    23041 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    23101 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    23161 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    23221 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    23281 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    23341 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    23401 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    23461 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    23521 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    23581 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    23641 ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag
    23701 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    23761 gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg
    23821 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    23881 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    23941 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    24001 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    24061 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    24121 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    24181 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    24241 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    24301 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    24361 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    24421 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    24481 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    24541 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    24601 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    24661 cactgcaacc attttcccgc ttccagctgg ggtagcttct gtcatataag cgcctctttt
    24721 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    24781 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    24841 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    24901 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    24961 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    25021 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    25081 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    25141 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    25201 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    25261 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    25321 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    25381 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    25441 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    25501 tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    25561 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    25621 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    25681 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    25741 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    25801 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    25861 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    25921 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    25981 tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    26041 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    26101 cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt
    26161 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    26221 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    26281 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    26341 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    26401 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    26461 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    26521 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    26581 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    26641 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    26701 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    26761 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    26821 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    26881 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    26941 tttttttact atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag
    27001 catttataat ttcttttatt agaaaaatca tctatctcac atattttgat tatcaaagta
    27061 ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca aaatttgtta
    27121 tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct
    27181 ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca tctatgttgg
    27241 taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata aacagcattc
    27301 aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg tagtagacac
    27361 ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc ctttttataa
    27421 ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata agggatttgt
    27481 attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc
    27541 gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt
    27601 gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa
    27661 ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag
    27721 acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat
    27781 cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt taagaacacc
    27841 atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt
    27901 ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc ctgacttcta
    27961 ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga
    28021 tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt ttgaaattga
    28081 actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttc
//
LOCUS       DACCSK010000027        27983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCSK010000027 DACCSK010000000
VERSION     DACCSK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.027"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(78..302)
                     /locus_tag="IVP39_001003"
     CDS             complement(78..302)
                     /locus_tag="IVP39_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7586088.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(500..2080)
                     /locus_tag="IVP39_001004"
     CDS             complement(500..2080)
                     /locus_tag="IVP39_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7586089.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2481..3857)
                     /locus_tag="IVP39_001005"
     CDS             complement(2481..3857)
                     /locus_tag="IVP39_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7586090.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3985..5103)
                     /locus_tag="IVP39_001006"
     CDS             complement(3985..5103)
                     /locus_tag="IVP39_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7586091.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5245..5679
                     /locus_tag="IVP39_001007"
     CDS             5245..5679
                     /locus_tag="IVP39_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7586092.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5757..6290)
                     /locus_tag="IVP39_001008"
     CDS             complement(5757..6290)
                     /locus_tag="IVP39_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7586093.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6384..7562)
                     /gene="mutY"
                     /locus_tag="IVP39_001009"
     CDS             complement(6384..7562)
                     /gene="mutY"
                     /locus_tag="IVP39_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7586094.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7555..8352)
                     /gene="recX"
                     /locus_tag="IVP39_001010"
     CDS             complement(7555..8352)
                     /gene="recX"
                     /locus_tag="IVP39_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7586095.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8459..9841
                     /gene="rlmD"
                     /locus_tag="IVP39_001011"
     CDS             8459..9841
                     /gene="rlmD"
                     /locus_tag="IVP39_001011"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7586096.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10015..10593)
                     /locus_tag="IVP39_001012"
     CDS             complement(10015..10593)
                     /locus_tag="IVP39_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7586097.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10915..11385
                     /locus_tag="IVP39_001013"
     CDS             10915..11385
                     /locus_tag="IVP39_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7586098.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11493..12719)
                     /locus_tag="IVP39_001014"
     CDS             complement(11493..12719)
                     /locus_tag="IVP39_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7586099.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13473..13673)
                     /locus_tag="IVP39_001015"
     CDS             complement(13473..13673)
                     /locus_tag="IVP39_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7586100.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13807..14415)
                     /locus_tag="IVP39_001016"
     CDS             complement(13807..14415)
                     /locus_tag="IVP39_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7586101.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14500..14970)
                     /locus_tag="IVP39_001017"
     CDS             complement(14500..14970)
                     /locus_tag="IVP39_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7586102.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15018..15932)
                     /locus_tag="IVP39_001018"
     CDS             complement(15018..15932)
                     /locus_tag="IVP39_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7586103.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16078..16881
                     /locus_tag="IVP39_001019"
     CDS             16078..16881
                     /locus_tag="IVP39_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7586104.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17059..18006
                     /locus_tag="IVP39_001020"
     CDS             17059..18006
                     /locus_tag="IVP39_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7586105.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18074..18367)
                     /locus_tag="IVP39_001021"
     CDS             complement(18074..18367)
                     /locus_tag="IVP39_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7586106.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18395..19060)
                     /locus_tag="IVP39_001022"
     CDS             complement(18395..19060)
                     /locus_tag="IVP39_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7586107.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19200..19832)
                     /locus_tag="IVP39_001023"
     CDS             complement(19200..19832)
                     /locus_tag="IVP39_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586108.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19839..20906)
                     /locus_tag="IVP39_001024"
     CDS             complement(19839..20906)
                     /locus_tag="IVP39_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7586109.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20903..21634)
                     /locus_tag="IVP39_001025"
     CDS             complement(20903..21634)
                     /locus_tag="IVP39_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7586110.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21803..22282)
                     /gene="greA"
                     /locus_tag="IVP39_001026"
     CDS             complement(21803..22282)
                     /gene="greA"
                     /locus_tag="IVP39_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7586111.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22418..23593)
                     /gene="mltG"
                     /locus_tag="IVP39_001027"
     CDS             complement(22418..23593)
                     /gene="mltG"
                     /locus_tag="IVP39_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7586112.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23797..24966
                     /locus_tag="IVP39_001028"
     CDS             23797..24966
                     /locus_tag="IVP39_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7586113.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25233..26855)
                     /locus_tag="IVP39_001029"
     CDS             complement(25233..26855)
                     /locus_tag="IVP39_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586114.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            27352..27983
                     /locus_tag="IVP39_001030"
                     /pseudo
     CDS             27352..27983
                     /locus_tag="IVP39_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         8601 a         5494 c         4463 g         9425 t
ORIGIN      
        1 aaattgcttt tcaaattttt gtgaattcca gcttatttct tggagttaaa cgcttctgtc
       61 tcagcctctt tttctttcta gctttttgat ttttctcttt tcttttgtac ttttctcttt
      121 tcattttcag ttctacgcgt gtctttattt ttaacttttt cctctttatg gatgattgat
      181 cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt ttacatctaa taatcgacat
      241 aatcgtaaca tctcttcttt tttctgtttt tttctttcac gtctttgcca ttctgtttcc
      301 atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa ataattttta tatttttttg
      361 attagacatt tctatgattt taatatagta aaaagagccg tcacggaaaa tccgtcacgg
      421 ctctaaacaa aatatgagag gctgtttttg tttggctatt cttattactt gatgcagggc
      481 agcttgtgtc aagacttctt tacatcaaac tttttaattt tacattggga tatttatccg
      541 caaaccagcg ttctgcaaat tggttctcga ataagaacaa cggttggtcg aaacggtcac
      601 gagccaagat attgcgactt gaactcatct tctcatccaa atcttctgga tcgatccagc
      661 gagcgatttt gtgtcccatt ggagacatga ttacttcggc gttatactca tttaacatac
      721 gatattggaa tacttcgaat tgtagctgac caacagctcc aatgatatat tcttccgtta
      781 gatatgtttt ataaagctga atcgctcctt cttgaactaa ttgatagatt cctttgtgga
      841 aggatttttg tttcatgaca ttttttgcag tcactttcat aaacagctct ggagtaaatg
      901 aagggagttc ttcataagca acttttagtt ttccttcgaa caatgtatcc ccaatctgat
      961 aattgcctgt atcatataca ccgatgatat cgcctgctac tgctttttcg acgttttctc
     1021 tcgaatctgc cataaattgt gtgacattgc ttaatttcag ttttttattt gtacgttcca
     1081 accagacatc cattcctcgt tcgaatgttc cagaacagat tcggacaaaa gcgattcggt
     1141 cacgatgggc tggattcata tttgcttgga tcttgaatac gaaacctgaa aattcttcct
     1201 cataaggact taccatctga tcatcttgtg ttttgtgtcc ataaggagct ggtgccaatt
     1261 caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa ccaaaaaata
     1321 ctggtgtctg atcgccacga gcaatttttt cttgattaaa tgcatctcca gcctctttga
     1381 ttagttctac ttcttctaag acttgttcat ataaggaatt ttcagttaga ggatgatttt
     1441 cgggaagcag tccatcttca tccaattcta ctaatcgttc atcctgataa ttttccggac
     1501 gataaaactc tacacgatta tggtagatat cataaagacc ttctagccct ttacccatac
     1561 cgattggcca attcatcgga taagattcga tatctaatag ctcttctaat tcttctaaca
     1621 attccaatgg ttcacgacca tcacgatcta gtttgttgat aaaagtaaat atcgggatgc
     1681 cacgtttttt tacaacttgg aataatttct ttgtctgtgc ttcgatccct tttgcactat
     1741 cgattaccat gaccgcacta tccacggcca tcaaagtacg ataagtatct tcagaaaaat
     1801 cttcatgtcc tggtgtatcc aagatattta ctcgtttccc ttgataatca aactgcataa
     1861 cagaactagt aacagaaatc ccacgttgtt tctcgatttc catccagtct gatttggcaa
     1921 aattacctgt ttttttgcct tttacggtcc cggcttgacg gatagcccca ccaaacaata
     1981 gtaattgttc tgtaatcgtt gttttcccag catccggatg ggaaatgatc gcaaaagttc
     2041 tgcgattgtc cacttgttgc tttaattttg gattattcat aacctaactc catttcttcc
     2101 attcaattat ttatattttc tctaggcaca gattgtccga tctcttaaag aaagacaatt
     2161 tcatataggg ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa acggcgttct
     2221 agagacagcc tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc tgtttttgct
     2281 tggctgctct tattacttaa tgcaggatag ctttttcaca acctttgtca actactacaa
     2341 ctgaatcagt atagcatgga ttcagcgaat gaaaaagcca ccaaaccata agagtcaaat
     2401 atttttttgt cagctgacta aaagaaaaga cgtgttgctt gtttattcaa gcaacacgtc
     2461 tttcataacc aagtttccaa ttatcttctt ggctcgtcat cttcgaattc ttttctgaag
     2521 tatcttcgtt cttcttcagg ttccgcttct gtttcttccg gatcatgaaa aatgacacgg
     2581 attttaagga ttcgagaacc ttccatttct tcagaaacca gcgtaatatt atcgacatca
     2641 aacgataact tttcgccttc atccggaatc atgcctaatg ctgtaatcaa ataaccagcc
     2701 atcgtatcta catcactcat atgcagatca ctgtcaaaag cttcattgaa ctcatcaatc
     2761 agcatacgtc cttggatgat atattcgtgt tcatcgattt tttcataaag attctcaact
     2821 tcatctgatt catcatcgat ttctccgacg atttcttcta aaaggtcttc tagtgttgct
     2881 aacccaacga cgccgccata ttcatctaat aaaatagcca tctgattctg cgtctttttc
     2941 atttcatata acagatcatc aataaagacc gtttcaggaa cgaataaagg ctcctgcatg
     3001 attttcttga tatctaaatt atcaaagccg aatttatggg cagcttttag taaattcttt
     3061 gtatgaagga ttccgactac tttatctttg tcttcgttat aaacagggat tcttgaatag
     3121 ttttcggata aaacttcatt cacattttct tccacagtgt cgttgatatc aatcataaat
     3181 gcatccgttc gtgggaccat gacttctcgg gcaactttcg tatcaagaga aaaaacccct
     3241 tggagcattt ctaattcttc attattcaat acgccttcgt tttcaagcat ataacgcatt
     3301 tcatctctgg tcatttttga atcttcatca tcaaaagtca tcggcgtgat acgcgccaat
     3361 aaacttgtgg aagcagaaag cagccagacg aagggcttag ctactacacc aatcaagcgt
     3421 atcagccctg aagtgaactt tgcgacttct tctgatttat ttaaagcaat acgtttaggg
     3481 tacaattctc caaaaacaat cgatacatag gtcaaaatag ccagcacaat gatatttgca
     3541 atattttttg ccgctgtgcc gccacctaat accggagcta accgttccga taaagtatta
     3601 gctagtgaag caccagacaa aatgttgacc aaagtgattc ctacttggat tgtcgatagg
     3661 aaattatttg gattctgcaa tatttttaaa agcttttgag atttcacatc tccttcatca
     3721 gctttttgct cgattcgatt cttattgatc gaaacaacag caatttcgga agctgctaaa
     3781 aaagcattga tcaatgtcaa aacgatcaat aataaaatct gcgctaacag cgactgactc
     3841 tcagggtcag cattcataat cgcattctct ccttataaaa ttaattttta gtttacgata
     3901 gaaaaaaacg tggcactata gccacgttaa aatggtatca caaagcaatt ttctttgcaa
     3961 taaaaacaag tgtactaaaa ctacctacga ggtcgtagca attttcttct tgttttcatt
     4021 ttgcttgtct tctttgacga ttttgattat ataggaaatg atcgtcttgc agattgcgta
     4081 aaaaggaaca cctaagaaga taccaagcaa tccggcaata tttccagcta ctagaagaac
     4141 gataatgatc gttaatggat ggattttcag tgatttccca attacattag gataaatgat
     4201 attcccatcc aactgttgga cgattagcac aaccacacaa caaagtaatg cttttaccgg
     4261 atcattgaag actgttacta gaaatgcagg tgctagtcct agataaggtc ctaaatatgg
     4321 aattaggttt gtcaatcccg caatgacccc aaataagaaa gcataatcca caccaattac
     4381 catatatcct aaaaatgtga aggtaccaac gaataaacat tcgattgctt gtccgctaat
     4441 gtaattagcc aatgttttat tcagttgccc caataactcc acaatttggt ttcttctttt
     4501 ttctgggaaa aactgttgga tgtttggcac tagtttttct ccgtctttca acatatagaa
     4561 caagatgaat ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc caacgatcga
     4621 acctaaacta ttggatactc cactaaggaa ttgctgaatg attgtcccgt aagaaatgtc
     4681 taattgctca aaatatttat tgatatctac ttccttgaac aaaggcaatt cggctacctc
     4741 ttttaaccag ctttctacct gtttgatgaa atcaggcata ttagatgcca atgaggaaat
     4801 ttgctgtact agacttggga tgacactaag taaaatccaa ataattgctg caactaaaag
     4861 gagcagcaca attgcgacag cccatattct tttgattttt gctttcatca acagcttgac
     4921 aataggattc aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa ataacgtgga
     4981 aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct gatgaaatca tgatcaatgc
     5041 agctatgatc aataattcaa gcgaccaaaa cattagttta gactgtctaa gtttttcaaa
     5101 caatagattt cctcctttct ttcttactag tatatattat attcgttaaa aattctttta
     5161 ttcttttttt atttttgaaa acttgccata ctttttattt gttttatttt ccagtatact
     5221 atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa caaaagatac aatgagcaat
     5281 atttacttag atgctgtacg gatcaggcat caagtttttg tagtcgaaca gggtgtgcct
     5341 ttgtctcgag aaatagacaa agatgaagcc cattgtatcc acttcgtcct gtactcagat
     5401 aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa aatgaaatta
     5461 cagcggatgg cgattctttc tgaatacaga caccaagggt taggaaaaat attgattgaa
     5521 gaagcagaaa actttgctaa gaatcaaggc tataatacga ttttattagg ggcacagtcc
     5581 acagctgaaa ctttttacga aaaactagga tatacagctt acggcgatcc ttttgaagat
     5641 gctggcatgc ctcatattta tatgaaaaag actttataaa aatttatttc gaaaaaagca
     5701 aaaaacagca agcttttgag aaaaatctct cactggctta ctgttctttt ttgtgtttac
     5761 ttacgcgaca attgcttgta acggttatac caaatatcaa tatagccttc cgagaacggg
     5821 ccatctccat tattgatcca gtcaaccaga atcttcacat tttcttttag gatgaagtca
     5881 atatcgttcg ggtaattcat cattttgctg tgcatctcat attcatcgac atccaatagt
     5941 cgtttctcgc cgtcaggaaa taccttgata tccaagtcat agtcgatata tttcaaggcc
     6001 tcatcgtcca gcaaaaaagg tgaagctaga ttacaataat aggaaacccc cttttctctg
     6061 atcattgcta ttacattgaa ccagtatttt ttatgaaaat aaacaatcgc tggttcgcgc
     6121 gttacccatc ggcgtccatc agattcagtg actaatgtgt gatcattcac gccgatcata
     6181 gagtgttcgc ttgtttttaa taccatggta tctcgccacg ttcgatgcaa tttgccgtcg
     6241 tgcttgtaac tttggatcgt cacaaactct ccttcttttg gaactcccat agcaaccctt
     6301 ccttaagcag cattttgtgc tttgtgtttg ggtgttcatt acaccgacat cataattatt
     6361 atatcaataa aaatgagctt tgcctagaaa ggattcctat tgtttcgatt tttctctaac
     6421 tgatctacca gtttcatttg gaccttagga aaaacgaccg aatcaaatgc atcaggcttc
     6481 aaccacttcg aagttttgtt ctcggtaaat tcttgttctg ctccctcggt tgcccgtccg
     6541 taaaaaagga gaacatgcca tttcagatga ctaaaaatat gagtaacttc tcctaaatgt
     6601 cgtgtctgcc aaacaaagag ttcctgcttt tcaaatggga gattttgctc ttcttctgct
     6661 actaggtcat caaacagatc gatttctggc tttgtttccc attctttttt caatcgctca
     6721 tactcctctt gtgtcacttc catcattgga aatgtccaca tattcgccag caatttttgg
     6781 ctatctcttt cttcaaaata atacgctcca gaatgatttt gaagagctgc actgatataa
     6841 taaacgtctt ttggctttgc tttttttgtt tttacaggaa aactcgtttg gattcctcgt
     6901 ttgttagcta aacaaaaggc ttggatcgga caagtctcac actttggaga agttggtgta
     6961 cagatagctg aacctagatc catcattgct tggttgaatt cacctggatg cttttcatca
     7021 atgattttcc gcatcgcttc atcaaagatt ttcctgctag aagcttttgc gatgtctgct
     7081 tcaatgcaaa acaatctgct gactactcgc atcacgttgc cgtcaactgc aggttctgga
     7141 agtccaaatg cgatacttgc aatcgcgcct gtcgtatatg gtcctattcc cttcaatgag
     7201 ctaatttctt caggcgtttg cggcatttcc ccatcaaatt ctgacatgat ctgtttagca
     7261 gcagcttgga tgttccgggc tcttgagtaa taaccaaggc cctcccatgc ttttaaaagc
     7321 ttttcttctg gagcatttgc tagctcttca attgtaggaa accattccat gaaacgataa
     7381 aaataatcga tcactgtgtc tactctggtt tgctgaagca tgatctcaga gatccagata
     7441 cgataaggat cacgattata gcgccaaggc aggtttcttt tttcttgttc ataccattga
     7501 atgaactgat cttgaaattc ttttgtttct ttatctgtcc atttttccca ttctttattc
     7561 actttcctct tccttttccg taatgaatcg ctggatcata tccaaatcaa atcctttttg
     7621 atataagctt tgctttactt ttagatttct ttttttaggt tcaaacctta aatttcttcg
     7681 ccatagttta tctccttgtt tgactaaata atcatattct atttcttgat ctgcttcttg
     7741 cggaagatca gctatcgctt gttggatcac ttcttgagaa aagcctttcg tcatcaatgt
     7801 ttgttggatt tttctcaata attctttttg gctttttccg tgattttttc caaacgcttt
     7861 ttcggctgtc tgatgtgcat tttgaacttg gtcttcaaat gtatattggc tgagcgcttg
     7921 ttcaatgatt tcaggtttca aacctttttg ctgcatctgc tgcgctaatt tctttggtcc
     7981 tttgtcactt aagcgcatat tcgtgcgcac ataactttcc ccatagacca aatcatcgac
     8041 aaggtccaat tcttttaagc gggctacgat tttatttcga tcttcttgaa ggatttcctt
     8101 ttcttttaag tatgttcgga tttctttttc cgatcgcaac tgataactta agtaattcat
     8161 tgctaggtga aaaccgaaat cagctccgct actcttcttg atctcttgga tagtttcttc
     8221 gtctaattcc cgtcctttta ataatcgata gcgtactaat acatcttcag atacttgtaa
     8281 agcttctcct tcagataaat ctacgcgata gaaagggcct ttgcctttac tgattctcgt
     8341 aacggttaac ataaacatcc tcctcgaaca tatattctct atcataccac agaaaatccc
     8401 cgtaatcagg tattatatgt tagaataaac cacagaaaaa agaaaaaggg agatacttat
     8461 ggcagaaaca attgtcaaaa ctggacaaac ggttcctttg aaaatcaagc gtttaggcat
     8521 aaatggtgaa ggaatcggtt atttcaaacg gttgatcata tttgttccat atgcactacc
     8581 aaaagaagaa gtactagtga aaatcacgaa agcaactcct cgctatgcag aaggagaatt
     8641 aataaaagtc aaaaaaacaa gcaaagatcg cgtagtggca ccatgtcctg tttactatga
     8701 atgcggtggc tgtcaattac aacacttagc ttatcaagct caattggatt ttaaaaagga
     8761 tctgcttttg caagcattgg aaaaattcaa accagcaggc tttcgatctt atcaactttt
     8821 gccgacgatc gggatggatg agccttggca ttatagaaac aaagcacaat ttcagttacg
     8881 aaaaaataag cagactcaaa aaattgaagc tggtttatac gaagcaaact ctcatcagtt
     8941 agtccctata acagactgct tggttcaaga acctcgaaca caaaaagtaa tgaatactgt
     9001 tgttcaacta ttgaataaat ttgatgcacc catctatgat gaacggacaa acagtggaat
     9061 cttccgcaca atcatggtta gaatcggtgt taaaactggg gagttacaag ttgtttttat
     9121 tactcgcagt aaaaaattcc cgcaaaaaaa cgcaatgatc cgtgagatca atgaacgact
     9181 gcccgaagtt gtttcaatca tgcaaaatgt ccaggcacaa aaaacgtctc tcgtgatggg
     9241 ggatgaaacg atccacttat ggggaaaaga agccattgaa gaacaaatca atgaagtgac
     9301 tttcgatctt tctcctcgtg ccttctttca actcaatcct cagcagaccg aattgcttta
     9361 tggcgaagga atcaaagcat tagatgtcca gcctaatgaa acgatcgtgg atgcttattg
     9421 cggagttggg acgatcggct taagtgtagc aaaacaagcc aaagaagtac ggggaatgga
     9481 taccgttcct caagcaatcg ctgatgcaaa aaaaaatgcg gaaagattag gatatcacaa
     9541 cactcgatat gaagtcggga cagcagagca agttctgcct aaatggttga atgaaggttt
     9601 tcagcctgat ggtatcatcg tagatccacc acgtacgggt ttggataacg cattgatcca
     9661 atcattattg aaataccctc caaaaaaact tgtttatatc tcctgtaatg tttctacact
     9721 tgccagagat ctggttaatt taagtaaaaa gttcaagatt gaatatttac aatccgtcga
     9781 catgtttcct cagactgcac gttgtgaagt tgtcgtgaag atgacgagac tcgctgacta
     9841 aatgaaagag gttgtgaaaa agtctgccct gtatcaagta ataagaacag ccaaacaaaa
     9901 atagctcttc atgttttatg aatactgtac ttgtgaccga agagttaaca ggaggacact
     9961 atttatttaa aaaaagggtt gtgacatagt ttttgtcaca gccctttttg attattattg
    10021 gatattcacg tctatcactt gataaaatgc atttgtcgta tcgctgatat tccatactgc
    10081 taaaatcaca ttatatcctt ttcgatcctg cggaagtgtg atcgtttggt tgactgtttt
    10141 tggtggaaca gttgctttgt cgtctatttt tgtgatcaat tcaaaatcac taaatttcaa
    10201 aggttgatta ggattccaag ttggtttggt aatgtagtaa tcccaagtac ttgtccgatg
    10261 ttgtgctgtt aaattccaag taatatttaa tgttcctgga ttaaccatgt ttttatgcca
    10321 tctgttagat gtctgttcat ctaaaggctc aaagccgctg actcctgcac tagcaatttt
    10381 tccatcaatg aatgtatttt tggtagcttc gatactttgc ggttcccatt gtgctcttcc
    10441 gacattttga tttaagtttc ctgctgctga tgttcctagg tatgcacggc ttcctggaga
    10501 agtaatataa ccatgcgcaa aacctggaat gctaaaaccg aacaacccta aactaactaa
    10561 cccaataaac aataacgtac taatcttttt catgtagaaa cctccttcat ttgataacca
    10621 cattgtaaac gctttattgt attaacacaa acattatgct tttataattt tatgttgaag
    10681 taaaaaaggt gaaaaaagta agatgcagat tataacagaa gtagacgaga aaagtacaaa
    10741 tagaaaacat gagaatcaaa tcatatgatc tttttcaaaa acatcattca tataaacaaa
    10801 tgttcaactt tttaataaca acaattatca atttttttct tacttcacaa actaaataca
    10861 ggtcttgctg tgagccttca tggtaaacta atattataga aaggagttag taaaatgaag
    10921 atgataaaag atattcgttt ttgggtttgt gttataggaa ttgtcatatt aggcttttta
    10981 tcagggttgc ttagcggaaa tcccggaaaa tattactatt ctttgcaact tccaccattt
    11041 gcaccaccta gctggatatt tgggccgatg tggaccttac tgtatatatt gatgggtatt
    11101 tctctttatt tgttacttaa tcacaacaac aaaaaacagc gaaacaatct tgtcggtctt
    11161 tttgtgattc agttcatttt taattttata tggtctgctc tattttttaa tttaagaaat
    11221 attttcattg cagcaatcga tattacactt ttagttattt tcttgtcagt gcttatgtat
    11281 cagttgtggc tgcatcatcg cctggcaatg tggctcatga ttccttatta tttatgggta
    11341 ctattcgcaa ctctattgaa ttattcaatt tattttttaa actaagcgct aaaaagagac
    11401 gtcctgtaat ttaacacata gaaaaagtgc cacctaagtt agattttttg tctaatttag
    11461 gtggcactct ttattaacca aactattttt ttctagtacc cacttgttgt tccatactca
    11521 ttattctggt cataaccata accattttgt tcctgtccag cgtaaggatc ataactttga
    11581 gtatcttgac ttggtgtata gtactgactt gtatcttctg tttgctgata ataggtattc
    11641 ggatcttggt actgatcata tgtgttttct gaataggaag aataatcggt gtacccttca
    11701 ctattcgatc catcaccgtt tgtcgaatcg ccatcttccg aatcatcaaa gaacatcatt
    11761 gaagcattct catttttcaa attaggtaat gtatcgcttg tttttatatt taattgtttc
    11821 ttcagttcat tttggatcga aagaagatca ttttttccta aaatttgata gtagatatca
    11881 ttgatcattt gtccttcgcc ttgcaactgg tcttgtttga ttgtttcaaa cgccggggtg
    11941 tagttcgtcg ctacatcaag catgtcatcc caatctaagt ccgtcgtcac attgttctca
    12001 actgctttta aaatttttct ataattgctt acgccatcta aacttaaaac tttattgacg
    12061 attttcgtca ctacttcccg ctgacgacgt tgtcgaccga tatcgccttc tggatcttcg
    12121 tgtcgcattc gagaataggc taaacctttt tctccattca atgtttgttc acctactggt
    12181 acatggacac catccaaagt aaagtcaatt ttattattca ctttgatgcc accgaccgca
    12241 ttgatcaaat cttccatgcc atccaaattg atcgttacat aatgatcaat aggaatatcg
    12301 agcagttgct caattgaatc catggccatt tcgacaccac caaatgcgta ggcatgattt
    12361 aatttatcta ccgttccata acctacgata ttcgtataaa tatctcggtc caaactgatg
    12421 atcgttgatt ttttttgttt cggattaact gttactacca tcatcgtatc agaacgtcct
    12481 tgttccgttc ttcctaatcc accggtatct aaacctaata gcaaaaccga aaaaggttct
    12541 ttgtcatcaa tgcttactct ctcagaacgt ttggaggata ttcgattaga gtttttagaa
    12601 attttgttta ctgtttggct cgcttctccc ataaatctga ttccgtatgc tgataatccg
    12661 gcaatcagca aaacgaatac ccctaacaag gttaatagaa ctttttgcca tcgcttcata
    12721 atacacctct gtttcacatt ttctaaaata ttgtctcata taactgttca atcagcaaat
    12781 tcacttttta atcataagaa aactttaaaa aacatttttg tgtaacgatt attatttttt
    12841 aaaatttctc aaaattctta actatcatac cataccgtga aaaagatcga aagaccgaat
    12901 acacgcagtt cgcaatcgtt ttatcttttt ttatgtattt tttccagaaa aagcgaacaa
    12961 caaggtattt tccaaataat cgtgcagtaa aatgatcgca catacctttc ccatcttttt
    13021 aacagtcttt gcctaccgtt cagattatca ttcgatttca cgacaatttc ttcctatgac
    13081 tggtcctttt gttaaaccca tcttctattc gttttatttc cagctcactg catggctctt
    13141 aaaatgactc gaactagtga ttgcattacc atcatctttt aatggataga tatgctcctc
    13201 ttcaaagtaa aacgattttc ttttaaagta atagaattga catggtaatg agtatgtata
    13261 ctagtggttt cacaaatggt cttttattca acatccaaaa actgaaattt ctttaaaatc
    13321 cattactaac gaagaaaatg ttccttgctt gataattagt taggacatgt caagtgaaca
    13381 aatgcattta cttagccaaa aaatctaaaa acttcataaa tagacaaaaa gacacgactc
    13441 tctaagaagt cgtgtcttta ttcaacaaat tattataatt tagtcacttc agcagcttga
    13501 ggtccgcgtg caccttcaac aatcgtgaat tctacttctt gaccttcttc taggctcttg
    13561 aagccgtctc cttgaattgc tgagaaatga acaaatacgt catctccgcc gtctactgta
    13621 ataaacccaa atcctttttc attgttgaac catttaaccg ttccgtgttc cataaatgta
    13681 tatcctccaa aaaagttctt gcaagtaacc ttgcaagaac attataccca atttagaaaa
    13741 cgctagcaag ctttcgctct tttattttcc aaattatttt taagactgat atgacggagg
    13801 aaatggttat tttgctgctt cgaaacgttt gtttacttca tcccaattaa taacattcca
    13861 gaatgctgca atataatctg gtcggacatt tttatatttc aaataataag catgttccca
    13921 aacgtccaaa cctagaattg gtgtctttcc ttccatcaat ggagaatctt gatttgcagt
    13981 agaggtaata gctaattttc cattttccat tacaagccaa gcccatccag aaccaaatcg
    14041 tccagcggct gcttttttga attcttcttt aaaagaagaa aaatcaccaa aagcttcatt
    14101 aatcgcttct tttatttctc ctgtaggttc gccaccagca tttggtgcca tgatttccca
    14161 gaaaaatgaa tggttagcat gtccgccacc attgttacgt acagctgtct tgatatctgt
    14221 tggaatagcg tccatatcag ataataattc ttctattgtt ttttcgccta attctgggta
    14281 tttctcaatt gctgaattta aattcgtcac ataagtattg tgatgcttat catgatgcag
    14341 atgcatcgtt tcttcgtcaa tataaggttc caatgcgtca tatgcataag gtagatctgg
    14401 taatgtgtaa gtcataaaaa aattcctcct caatgttttc ttaataatgt ctacattaca
    14461 aagagtaaca aagagaagga aattcttcaa ttgttatgcc tagtcgtctt tttcactttg
    14521 acctacttct tcttcaaaaa cctcagtttc tttaaaaata aacaacttgc tgaaaatata
    14581 gttcgcaagg ataacaataa tttgagtgat gattttcgcc acatactcat tgctgttcat
    14641 aacattgatc aacaatacca ttgcccccat gtccaatccg taagaaagga tacggtagaa
    14701 gaagaactta ccgcattcaa taagtgcatg tgtaaaattc ggtgattttg aatggaatac
    14761 ccaaagtttg ttcgttacaa aagcaaacaa tacagaacaa aaccacgaaa tagaattgga
    14821 taaggccata cttaaatcaa aatattggcg tgcgagagca aaactaacaa aatttacaat
    14881 cgtagccaaa ccaccaaaaa agagatagac tagtatctcc caatatcctt ttgcttctaa
    14941 atatcctttt aactgtcgat actgcttcat gagccaacct accttattaa aaattcttat
    15001 ttatcatact ctatatttca ataactttca tctttattta aaaaaattaa ttttttttga
    15061 tcctttgcct ttgtctctgc ttggatcgtc acatgtgtaa taccatattt taattttaat
    15121 aaagaaatta tacgaggata tatcttttca atctcgctta gtagctgatc ttcaaaatca
    15181 atatgtccgt cgaaaataat tatcttttcg ctatactgcc aaatatggac atgatgaaca
    15241 tcctttaccc catcgattgc ttcaatttct tgttccattt tttcatagtc gatagcaggt
    15301 gcactttgta tcaaaatagc ggcagctttt ttgatcacca ttatcgcttc tatcaatata
    15361 tatatcgaaa tgatcagtgt aataactgga tcgatccagt agatatcgaa aaaacggatc
    15421 aaaattgctc cgataatcac acttaccgaa gacagtgtat cacttaacaa atgcaaataa
    15481 ctagatttta tattcaaact ttcatctgct tcttgtcgta ataataatac tgagaacaaa
    15541 ttcgcgagca atccaataac tgctactgtt agcatcaaat gactatttat cttttctggt
    15601 gaagtgaacc gcttgaatgc ttcaacggtt aaaaacaagg ataatatgat caatatgaat
    15661 gagttgatga aagcactcaa tatttctaaa cgctggtagc catacgtacg ttttttattt
    15721 gcttcttttc gggccatttt ttgtgccccg taagaaaaaa taattgccaa tgtatcacta
    15781 aggttatgga aagcatctga aacaagagac aaactccctg acataatccc tccaataaat
    15841 tctgataaag tgatcaccgc attcaaaacc actactccaa atagtttttt tgatgatctc
    15901 tgtggctgtg taggcactat tccttcctcc attttattta tattggatag tctttagaca
    15961 agaataacat ttttttgata tttcaccaat cattcagctg tttccttaaa gcacttgtat
    16021 ttcacttgat acttcgctag aattgcaagt gatgacacta ctagatgagg agtacttatg
    16081 acaacaaaac aattaattat cagttccttt atacgattca aagacctaaa ggaggcgaga
    16141 aatgtcccat tttggaaagc cattgtctat cttttagttt tgagtataat tatggctttg
    16201 ccaatctctt ttcaagtttt tcaagtctta aacaacattc aattggatgg gcaaaagatt
    16261 gctactcgca ttcctgattt tacaatccaa aacgggcagc ttcaaaccga agaaaaagaa
    16321 ggattcattt accaaacaaa ctccattatc ttcacatttg atccagaggg aaaacgaaca
    16381 gaacaggaca tctctactga tttgatgggg aattttgtta gtgtcgggat gttaaaaaac
    16441 aaacttatca tagctcttcc taatacaggc acgacttctg ctttgttgaa caataaccaa
    16501 ttagaacttc cctacaccaa tgaatcttta aaaaacttaa caggtaagca gctccgttct
    16561 tttttaagtg aagcatctat accactttgg ataaaagtcc tgacatttat attttctatt
    16621 tatcctagtt ttctgaatct gctgatcact ttgctattta ccaacattgc tgcttttctc
    16681 tatgcacgct ttcgactaac aaaagctact tttcttgatt gtttaaaaac attgatctat
    16741 tcaatttcat tgcctactat aattgcaaca atcttgatgg tctttcttcc ttcttttgac
    16801 actagcgcct tcattgcttt tgcaggaatc tttatttttg ctcaagctgt caaaggatgg
    16861 gcaaaaatat cgatcaatta agaaataaag acaactcgtt atttctactt caatagaccc
    16921 ttttagaggt tgtgacattt ttttgtcaca gccccttttt tatttgaaaa atatttcttt
    16981 ctcaaaaaac aagcaaggcg cttcaaagtt cttcttgtat gcgctacact tagagtaaag
    17041 aaaggcggga aattaactat gtcagaaaga atcatttatg cagatcgtcc atttcgtgat
    17101 gaatttatcg aagaaatcca tactatagcg cctggttata tctttaaaac agaattacaa
    17161 ccagaggatc tacaaaatgt agagatcagt ttgggctgga actcagacta ccaagaaaaa
    17221 ctattagcct cttctacctt gaaatgggtc caatctatct cagctggtgt tgatcagcta
    17281 ccccttgaga ctttttcaaa gaaacatatt ttactttcaa atggtagtgg catccacagt
    17341 gaatcgatta cggagcatgt cttaggaatc atccttgggt attctcgtgg tctttttcaa
    17401 gcacaaaagg cacagagtga catgaaatgg ttaggttcat cggtccatta ccaatctatc
    17461 caagggaaaa aaatactaat cattggaact ggccatattg gaaaaatgat tgcaaaaaaa
    17521 tgcaaagcat tagacatgaa ctgctatgga atcaatacat ccggtcatcc agcagaagga
    17581 atggaaaaga ctttttcttt aaatcaacta aaggaagttc ttcctgaagt agatttcgta
    17641 gtcaatatct tacctctaac ggaggaaaca actgggctct ttgatcagca tatgtttgaa
    17701 cactttgatc caaaatctgt attcatcaat gttggccgtg gtgcttctgt taagacacaa
    17761 gatctggtgc aggctttaaa taaccagcaa ctttcctttg cagcattaga cgtttttgag
    17821 gaagagcctc ttccacaaga tcatcctctt tggaaaatgg acaatgtact gattacttcc
    17881 cacattgcag gtttgacacc tgattttcaa aagaaattaa tggctatttt tttaaccaat
    17941 ctgaaaagct actttgccac ccatgagctt caaaccaatc aagtaaaatt gtctgcagga
    18001 tattaatagc tgaaaacaat attttcaaaa gaaaaaagag aaggtgacaa aaattgtgtc
    18061 accctctctt tttttattgt tttttaaagt gataggttgg ctcctccagg ttgggatcat
    18121 aatcaacaag taaatcatat cctttaaaga accattcatc tgcttcctcg ataaaaaaaa
    18181 gtgtcccatc catttccact ttctttacag gatgttctgg ctgttcaact gacattccaa
    18241 tcgaaaaacc atcatgaacc tctgttttgc cgtagacttt tccaaaaaaa cgtattccgt
    18301 aagtttctgg cagatcaatt tctgagtcaa accacttttg tgcttttggc gttaccgtta
    18361 atttcatccc aacaacctac tttctctttt ctttctattg attcatctta tcgtctagaa
    18421 tatccacatc gcctgtctct ccaataacca gtagattatc attttcacga acatattcat
    18481 ctgctgcagg agaagcgata accgtttgat ctactcttcg tatggcgact actgttaagt
    18541 caaaacgttg cctgaaattt aattcagcta atgttttgtt gaaaaattta ggattggaga
    18601 ctcggatttc tgctaaagaa tatttatctg acaactctag gtaatctaaa atattctttg
    18661 acactaaatt atgcgcaatc cttattccca tgtctcgctc gggatggacg acgcgatcag
    18721 ctccgatctt ttcaagtact ctggcatgat actcattttg ggcttttgct aaaatatttg
    18781 gtacgcccat ttcctttacc atcagagtaa ctaagatact ggcttgaata tcttctccaa
    18841 tagcgacaat tacatgatcg aaatttctta tgcctaatga tcgaagtgtc atttcatcct
    18901 gtgcatttgc cacaacagca tgagtggcta tattcatata ttcattgacc cgatcttcat
    18961 tactatcaat agccagtact tcttgaccag cttcgatcag tgttcggcag atacttccgc
    19021 caaatcgtcc aagacctaca attgcaaaat tttgtttcac cgttttcctc ctttagtatc
    19081 ttcttttctc agtataacaa agcctttaca aaaacaaaag caagcatcat tattgtaaaa
    19141 aagccaagaa aaataatagt tattttctca gcttttgatt atcctcttta tcatcgtttt
    19201 tatttcacca aaccatgttt gaatgcatag atagcggctt gtgtacggtc ttccacttct
    19261 agcttagcca atatattgga aacatgtgtt tttactgttt tcagtgtaat aaacaattcg
    19321 tccgctattt cctgattact ttttccttca gaaatcagca ttaaaatttc attttcccga
    19381 ttcgttagtt cttcatgtaa tacaggctca tttcgccgac tcattttttc catcattttt
    19441 gttgtcactt ctggttctaa tactcgctcg ccacgttgcg ttgcccttat agcatcagca
    19501 atttcatgtg cggtagaggt cttcaacaaa taccctgctg cacctgcttc aatggcagga
    19561 taaacttttt cgtcatcaat aaaactggtt acgataatga ttttagcttc tggccaatct
    19621 tttaagattg cttttgttga ttcgatacca tccatttctt ccatgactaa gtccattaaa
    19681 atgacgtccg gtcgaagtgt catcgccttc tcatagcctt gtcgtccatt ttcagcttct
    19741 ccaattactt caatgtcttc ttgaatagat aaataagaag agacgcctaa gcgcaccatt
    19801 tcatggtcat ctactaataa aacttttatc atccttatct atgcctcctt catcaaaggg
    19861 accttgattt ctacacttgt accttgacct ttaaaactaa tgatttttac tgttccacca
    19921 ataccagcta ctcgttcttt gatattgttc aatccataac ttcccgtttt cgtttcattc
    19981 atattaaacc ctgttccgtc gtcaataata cgtaacaaaa gattattgtc tattttgtgc
    20041 aaatatacct ctaattcatt ggctttagca tgtcttaatg tatttgataa caattcttga
    20101 acgattcgga acaagtgatc ctcaatggaa ctagatagtt ttacatcttc tacatcccat
    20161 ttcagcgaaa tctggatttt attttgcaat tccttcaata actgctcgat accttgcttt
    20221 aaacttttct cttctaaatt gactggacgt agatgaagaa gcagcgcacg catctcagac
    20281 tgggaagcgt tgatgatctc tgctaccatt ttcaactgct tgcgaaacat ttctggtgtc
    20341 tcgctttttt ctgcttgctc cgtcaatgcc gacatcatca tcattgctgc aaacaattgt
    20401 tgcgaaactg aatcatgcag ctcacgggct aatcgatgtc tttctaattc taaaatttct
    20461 tctttggttt gtccatctac atattgggga cggctcgtta caatttgtaa ttcactagat
    20521 acttcaatca ttttttcttt tatcttagtg atttccttat caatatcttt gatgtataaa
    20581 tcgtccgatg cacttgggat cggaagaagc aacaatttgg attcataatt gccagaagaa
    20641 agtgcacgca gcttttcttc gattttcccg tattttgtct tttgaataag tgataataat
    20701 agaaagacaa ttaatcctac acctaaggaa atggctaaca aatggaagat aagcggaaca
    20761 tataataaac gtgcacgcag taattctccc caccaatgac tttgattgct ggcataaaaa
    20821 taagtgaaaa gtgaaaatag aataataagg aaagccgcaa tccccgaata aacagctagc
    20881 atcgctctgg atatttttcc catcatattc gtatcacctc gacatctcca atcaaggtat
    20941 tagtaataat cttcaatcta cgagggtttt catcataatc actgctgtag attttgattt
    21001 gttcattttt caaagaaaat tgctcttctt caaacaagac atttcctact aatgtcgcat
    21061 gctctaatcg gatggctacg cccaaaggta ctaagatccg tgttctgcct atcccttttc
    21121 ttacaatcac aatattatca tcttttggca aaagcgtatt gcccaaatct atgatagtat
    21181 caccagaaat gacggcgatg ttgatatcgt cccattcata gacgtcgttt ccaatacgtt
    21241 ggttaccgaa taattgctgt cttttccgct cattattatg tgtttttaat tgctctgttt
    21301 ggatcatcat gatctgtttc tttcgccaaa aagcattttt ggttaaatct atccctgaaa
    21361 tctctacacc tttcagacca ataaataaaa cggcaaatac agccatcatc cataaagcag
    21421 gactgttgac taagctaaaa aaaatgacca aacttcctaa tatcaattga aaattttgaa
    21481 atttagtacg aggatgtttg cgcatagcta gatatatatt gaaaataccg aatatcacta
    21541 aaagcaaaag tccagtattg tttacgattt gccaaacagc aaataacagc agtagtgctt
    21601 caactaccac aaaaaaacgc caagaactat tcatcgctct ttctccctcc tgacacattc
    21661 tcatttcatc atacctattt ttttaactgt tggctatagg actttagatg taagatcgtt
    21721 tgttgaaatc taaaatacac aaagagaaaa agatctagag tgtatcatca gtaagatgaa
    21781 ctctctagat ctttcagtca gactacgctt gttctacttt gacgatttta actaacatat
    21841 cgccacctgg agtagcaata gttacttcat catttttacg tttgccaatc aatgcttgcg
    21901 caataggaga atcattggaa attttgccag aaaacggatc tgcttcagcg cttccgacaa
    21961 tcgtgtactc ttcttcttct ccatctggta gttcgatgaa tgtaactgtt ttaccaattg
    22021 atacctcatc tgcatctaca ccgtcgttat cgatgatttg agcaaaacga atcatatttt
    22081 caattgttgt gattcttcct tcaacaaatg cttgttcatc tttcgctgat tcatattcag
    22141 agttttcgga aagatctccg aaaccgcggg caattttgat tcgttcaatg atttctccgc
    22201 gtttgactgt ttttagttct tctaattctt tttctaattt ttctttacct tctagagtca
    22261 ttggataaac tttctctacc atgtgtaacg tctccttttt tcgataaaat accgcaaaag
    22321 gtaatctttt gaggaatata gtccaaaaaa gattaccacg cttttcttca aatgtaaatg
    22381 tttttctaaa aatgatcaat gtctagagcc tgtttttcta agaattattt acatattttt
    22441 ccactaattc catatgttct tcataagttt tagcgaaata aacatttcca gtgctcgtat
    22501 ctgctacgaa ataataatag tcattttctg ctggttctaa tactgccttg attgcctctt
    22561 cgcttggatt gtcaaaaggt ccaggtccat agccggtatt ggtatacaaa ttatatggtg
    22621 aatctacagc tgtatcttca tacgtcacta attctttgtg ttcacctaat gcgtataaaa
    22681 cagagatatc tgactgaagt ggcatatttg ctttgattcg gttaaagaag acttgtgcaa
    22741 tgtttttacg atcattttct tttacgcctt ctttttcaac tagagaagct agagttaaaa
    22801 cttcttgtac agtcaaattt ttctgcttga tagctgaata atagttttgc atgaccgtat
    22861 tcgtcttgtt caccatttca atcactagat ccttaagcga agtactcgtg taataatcat
    22921 atgtggctgg gaataagtaa ccttccaaac ggtattttgt gttctcagct ttagatgcac
    22981 tttccaacag ttctgggaat ttttggtgca actcgttaaa gaaagtctca tcattcacta
    23041 aatctaaaaa ttctttcttg tctttaccag ttaccttcgc tacccgttca gcaatctgat
    23101 cgatatcgta cccttctgga atggcaattt tcccatctgc aacttttgtt ggctcagaag
    23161 taccgccttc ttgcaactgt ttgccaatct cgtcaagagt catattaggc gctaactgat
    23221 aatagccagc ttgaaagcca gttaaatttt taaattttgt ataataattg aaaacaatcc
    23281 cgcttttaat aatattgtcc ttctccaaaa tctcaccaat ctgcttgtta gaagagccac
    23341 tcgggatctc gacttggact aattggtcat ctgatttatc taaaggcttc aacccgctat
    23401 ctacataacg atacacggta aatccaagaa atccgccaat aatcaataat gtaagagcaa
    23461 tcactaaaat gatcttcctc acaatcttat cttctttttt acgcagattt ctccgcttgt
    23521 cgattatatc tgctgtagct gccttactgg cttctttttt atctttactt gggtgttgtt
    23581 tgtttcctga caaaacaact cctccttact atcaagttcg taactttatt cattatacat
    23641 gatatttgaa ttggcgtgaa tgattttatt taaatattct atcaaaaatg taagtagtaa
    23701 atccattact ataagattaa aataaatgtt atcaagatac aagctatttt agctttattc
    23761 tttctgtaaa aaagaaagga gactaaataa actattatga aaaaaggaaa acatgctaaa
    23821 aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct
    23881 acaggaacta ttcttggatg gcaattttac aataatgaac actctcagat agttgcccat
    23941 gctcaatcag ctcaaaaaaa gttgatactg actaccaatg aacaacagct cgttgaaaaa
    24001 atcgatcaac tattaagaaa aaatgattat attggctcaa tttacgtaaa gaaaaataat
    24061 cgcatcatca tggaaaaagg ctatggctat gcgaataaac tcacgcaaca accaaatgat
    24121 ccttctcttt attatcagat tggttcggtt caaaaagcat tgactactct tttgatttta
    24181 aaacaagtcg agcgtgggca cctttcttta gatactaagc tggctcaatt ctatccacaa
    24241 attgctggca gccagcaaat tactatccgt gacttgctgt atatgcgctc aggcttgcac
    24301 cgaactgctt ctccaaccat accgatgact gatgaagaag tcgttcaatt tgcgattcag
    24361 cacttaaaat ttacagatta tcagacctct cattatgatt cattgaactt taccatattg
    24421 actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac cgatgatatc
    24481 atcgaacctt taggattaca gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat
    24541 catgcactat cctatcaaat gtctaaagag gatgattact ttaaaccact gacagaatct
    24601 gaaacagaga tccataacga attagggact ggtaatatca gtatgtccgt ctatgattta
    24661 aatacgctgt ttacacaagt tttatctgga aaactagttt ccaaagactt actcttctca
    24721 ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg gggtatatag cggagataat
    24781 tatatcctgt cgcaaggaaa tatcgactct tttgatacat ctgctatatt tagcaaagat
    24841 tcacaggatg ctatcatcat ggaaagcaat ataaaagctg acaaaactat caaactgcct
    24901 actgctgatc taagaaacca aatctatgac ctgatggaag gaactactaa gttggcaaat
    24961 agctgatatg atgaaaagat agcaaatact acttctttga atagcatttg cttaaaaaaa
    25021 gtactcgact aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt tttgctacag
    25081 actttaattc caaataaaca gaacagaaag aggtgttctt ctttctgttc tgtccaaatt
    25141 attctaagat ttctctttac caacattatt tgacaaaggg cctattaaat ctaaaatagt
    25201 atctttagat tgctaaaata tgtgctagca aatcattcat ggcttgttct tatttttaat
    25261 tttatgcaat aaaaaagaag aacaattagc gtgattatag aaataaataa tgagatttta
    25321 gaaaaaaatg tgtatgaata cctgattgtt actttattta ttccactttc actttttact
    25381 gtaacaaaag gacctccttg ttttagtact gtagcatttt ttgaattaac agtaaccgtt
    25441 aatcctttat accggaatac aggtaaaact attgttttgc cttttgaaga tttaaacgta
    25501 aatgtaattt catctttatt tggagaaaag ctactataaa taacttgacc atcttctgta
    25561 acttgatggt ttttcaaaag caattgatct tttttatagc ctataggtac ataatcaaaa
    25621 ttctcaaatt cttcaagttt attttccatt tgttcattag aataagttcc ttgaaaattc
    25681 atttctttca ccatgttaac tactgatgaa taatttaaaa ttgaaatagc taatattaat
    25741 aatagtataa aatttcttct ccttgtataa gaaaattttt ccaactgtgg agaaataaat
    25801 agagatactg aaaaagatat gaacaatgtt gaaaatatca taaaacgcca aggagcttga
    25861 ataatattta aaggagtatt ttgtaaggca aaccatggaa atatttttgt acaaagtaga
    25921 atagatatta tacccattat ataagtgatt ttagataaga ttcctaattt tttaaagtta
    25981 aatgttagta taattaaaca aataagcaaa gttaagccaa taccaaatga tagtaattct
    26041 gaatctatac ttccaatcaa aaactcttta cccagataaa ttgctctttt ttgtaatata
    26101 tccacataag gtctattcag atcttgatac aatgtctgct caatcatagg acctataaac
    26161 gctagtgaaa gaagtaaagt aactatagca gcttttatta atttaataat taagaagatt
    26221 tctattttct ttttccgata aaattgaata attaaagcaa ttataatcat taccatagtc
    26281 atagctacag aaagaacatg agtgtaaatt aataaagtca tgctaatcgc caacataacc
    26341 caagattctt tttttgagtc gtaaagcttt ataaacccga gtaaaataag aggaagtatt
    26401 gacattgcga gtaattcccc cattgcccct cgaagaaata tttctacact cctaaaagaa
    26461 gatgttgtat atataatagc gaaaaaggag ctagtaacat gttttttggt aatttcatac
    26521 attacataat gacatataaa tagagtaatg agcgtaacaa tataaagaaa aaggttatag
    26581 cctattgcta aattagtaaa cttgatcaat aaaaataaag gatatagagt taaccatggg
    26641 taaaaattat ttacttgcat gccatatgat ctaaacgttt caaaattaat aggggagcta
    26701 aaaatagatt ctaaacttaa cgcacgatta atgtgaaatg ccgtatcata aaagccaaat
    26761 atttccatgt ttttaaatgc tggaaataaa tataataaac taacaaatgc ataaactata
    26821 aatattttta cagaaaaaat ttttttcaaa ttcatatacc acctcatgtt tttttatatt
    26881 acatatagta tcatttattt ttttaaaaca ctagattagt cactatataa aatgtgatta
    26941 attttattat taataaaata ttttttatat tacaaaagta gtgtttcaaa gttagtagag
    27001 aaaatgtagt tatatattta aatttttacc agaaagaaat gaacaccttc aattatctaa
    27061 atctcgaaaa tggttagatg taatcatcag gaaaaacaat cttaaacgaa ttactattca
    27121 tggtcttaga cacacacatg ctagcttgct tttagcaagc tagtgcaaat attaaagatg
    27181 tgcaagagcg tttaggccac tcttctattc aaatcattat ggatctatat attcatatta
    27241 cagacaaaag aaaagaagaa accgtagcgc aattcgcaaa atataggctc tataatttat
    27301 gggactgatg aatcaaaatt gattcttcag tcccattgtt attttaggta tatgattcgt
    27361 acattgaaag agttgaaaaa ctatcttccg aagccgtcaa aaaggaccct gccaaaagta
    27421 ttgatggtgg atgaatttcg ttcgcacgct tcaattgtgg ataaaatgag ctttatttgt
    27481 gcggatggcg agactggaaa actcattgat attttgccta cacgaaaatt gtctcgatta
    27541 acacactatt ttagtcgctg tgcaaatcct gaagcggtgg aatatttagt gacagatatg
    27601 aacgccgcct acttccaact gacaaaacgt gtcttaccaa atgcgaaagt cgtcattgat
    27661 tgttttcata tcatcaaaca tttgaatcag gcgttcaatg attttcgtgt acaagaaatg
    27721 aaaagattac accagttagg gcaacgacgg gaagccgaga aagtgaaaaa gaactggcgc
    27781 tttcttctaa aaaaccgtca aaaaatcaat cactatgatt acaaaacatg gcgcagtttt
    27841 cgtgcaccta aatacccgct actcactgaa gcaatgatga ttgatcgatt gctggcattt
    27901 tcaacgcctt taaaggacgc ctatactttt ttccatgagc tgaccgaagc ctttagagac
    27961 aaggaatcag acttattttt tac
//
LOCUS       DACCSK010000028        27824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCSK010000028 DACCSK010000000
VERSION     DACCSK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.028"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1232
                     /locus_tag="IVP39_001031"
     CDS             <1..1232
                     /locus_tag="IVP39_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7586115.1"
                     /translation="VAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAPVF
                     HGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGVPH
                     DQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALANTG
                     GAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLVNV
                     PMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTVAT
                     GGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIPFA
                     AADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGAVV
                     SGVIYGLLRKAPAVAEA"
     gene            complement(1343..2902)
                     /locus_tag="IVP39_001032"
     CDS             complement(1343..2902)
                     /locus_tag="IVP39_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7586116.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            3057..5291
                     /gene="pcrA"
                     /locus_tag="IVP39_001033"
     CDS             3057..5291
                     /gene="pcrA"
                     /locus_tag="IVP39_001033"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7586117.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            5308..7344
                     /gene="ligA"
                     /locus_tag="IVP39_001034"
     CDS             5308..7344
                     /gene="ligA"
                     /locus_tag="IVP39_001034"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7586118.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            7450..7755
                     /gene="gatC"
                     /locus_tag="IVP39_001035"
     CDS             7450..7755
                     /gene="gatC"
                     /locus_tag="IVP39_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7586119.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            7755..9227
                     /gene="gatA"
                     /locus_tag="IVP39_001036"
     CDS             7755..9227
                     /gene="gatA"
                     /locus_tag="IVP39_001036"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7586120.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            9224..10654
                     /gene="gatB"
                     /locus_tag="IVP39_001037"
     CDS             9224..10654
                     /gene="gatB"
                     /locus_tag="IVP39_001037"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7586121.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            10664..11719
                     /locus_tag="IVP39_001038"
     CDS             10664..11719
                     /locus_tag="IVP39_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7586122.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            11830..13203
                     /gene="rlmD"
                     /locus_tag="IVP39_001039"
     CDS             11830..13203
                     /gene="rlmD"
                     /locus_tag="IVP39_001039"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7586123.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRAILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            13206..13334
                     /locus_tag="IVP39_001040"
     CDS             13206..13334
                     /locus_tag="IVP39_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7586124.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            13774..13962
                     /locus_tag="IVP39_001041"
     CDS             13774..13962
                     /locus_tag="IVP39_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7586125.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            14040..15053
                     /locus_tag="IVP39_001042"
     CDS             14040..15053
                     /locus_tag="IVP39_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7586126.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(15120..16154)
                     /locus_tag="IVP39_001043"
     CDS             complement(15120..16154)
                     /locus_tag="IVP39_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7586127.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            16268..18493
                     /locus_tag="IVP39_001044"
     CDS             16268..18493
                     /locus_tag="IVP39_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7586128.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            18511..20394
                     /locus_tag="IVP39_001045"
     CDS             18511..20394
                     /locus_tag="IVP39_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7586129.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            20375..21349
                     /locus_tag="IVP39_001046"
     CDS             20375..21349
                     /locus_tag="IVP39_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7586130.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            22008..23030
                     /gene="aroF"
                     /locus_tag="IVP39_001047"
     CDS             22008..23030
                     /gene="aroF"
                     /locus_tag="IVP39_001047"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7586131.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            23063..24127
                     /gene="aroB"
                     /locus_tag="IVP39_001048"
     CDS             23063..24127
                     /gene="aroB"
                     /locus_tag="IVP39_001048"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7586132.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKNLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            24129..25295
                     /gene="aroC"
                     /locus_tag="IVP39_001049"
     CDS             24129..25295
                     /gene="aroC"
                     /locus_tag="IVP39_001049"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7586133.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKVAVKEYRDYLKHY"
     gene            25312..26403
                     /locus_tag="IVP39_001050"
     CDS             25312..26403
                     /locus_tag="IVP39_001050"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7586134.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAI
                     EQQIVDYVANDFETGAEQADVILLAVPVRTTLAYLAVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADDKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMAQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPIHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            26416..27711
                     /gene="aroA"
                     /locus_tag="IVP39_001051"
     CDS             26416..27711
                     /gene="aroA"
                     /locus_tag="IVP39_001051"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7586135.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEMIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFMTTLFGDASLSKRPMGRVMEPLREMGADLQGKENEQYLPITVTGTNSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRISVEGKTITVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            27704..>27824
                     /locus_tag="IVP39_001052"
     CDS             27704..>27824
                     /locus_tag="IVP39_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7586136.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQRLQKEYVDLDTKIEEHI"
BASE COUNT         9206 a         5114 c         6135 g         7369 t
ORIGIN      
        1 tcgttgcagc agacaaaaaa gtagagatga atcgctttga cggaaaagaa ctgatcaatc
       61 gtcctgtcag tgatggtatc cgtaaaccag aagagttgat caatctagcc cttagtggta
      121 ctgcaccagt attccatggt gatggaaaag aaagcagttc agaagaagga aatgcggatg
      181 gtactatcgg acaaagaatt tacaaagatt tgatgaacgg cgtatcacac atgttacctt
      241 tcgttattgg tggaggaatc gctattgcgc tttctttcat gatcgaccaa tttattggcg
      301 tgccacatga tcaattatca aatctaggga attacaatca agctgctagc tggttcaacc
      361 aaatcggaca agctgcattt ggtttcatgc tgccggtatt agcaggattt attgcttcaa
      421 gtatcggtga ccgccctggt ttgatcgttg gatttgctgc tggtgcatta gctaatacag
      481 gcggtgcagg attcttaggt gcactgattg gtggattctt agctggttat gtcatcgtat
      541 ttttacgtaa attattcaag aacttaccaa aatcattgga tggaattaaa acgatcctct
      601 tttatccagt gtttggctta ctgattactg gtttcttgat gttgctggtc aatgtcccta
      661 tgaaagcaat caatgatgca ttgaacggtt tcttgacaag tatgagtggt tcgaatgctg
      721 ctttacttgg tgcattatta gctggaatga tggcggcaga tttaggcggt ccaatcaaca
      781 aagccgctta tgtgtttggt acagcaactt tagctacgac tgttgcaact ggcggaagtg
      841 tcgtgatggc ttctgttatg gctggaggaa tggtacctcc attggcaatt ttcgttgcta
      901 cacgtttgtt taagaataaa ttcagcaaaa cagatcaaga tgctggtttg acgaacatcg
      961 tgatgggact atcgtttgtc acggaaggat cgattccatt tgcagcagct gacccgattc
     1021 gtgccattcc aagttttatt attggttctg ctttaactgg cggattagtc ggagctttcg
     1081 gaatcaaatt actggctcct cacggcggga ttttcgttgt attcttactg agtcacccgg
     1141 tcatgtattt ggtgtttatt gctatcggag cagtcgtatc tggtgtgatt tatggtttat
     1201 tgagaaaagc tcctgcagta gcagaagcat agaaataaaa aagaagaggc tgggttttcc
     1261 cagcctcttc tttttttata gaatcatata gaatcaagta gtgtagatga atagttgttg
     1321 taaaatagtt tttccatgtt cgttaaaatg attgcttcaa atttgtgata gaagatttag
     1381 cctcgatttc ctgcttgatt cttggcaagt ttgcttcatc agtcaagata accaaaatat
     1441 ctcctacatt catgatcgta tcaccgtgag tcaagatttc ctgatttcct ctgcgtatag
     1501 agattagcag catattttta ggccaattga agtctcgtac cattaatccg tctaaagaac
     1561 tctctgccgt tactggaaaa tcaaaagttg tccttgcacc ttttagtgag gcggtctgag
     1621 tatttccaag tagacgttcc aataatgatt cgtaaattgg attaccgccg agaaggtcat
     1681 tgacgatata agcaacaaga gcagccaatc ctaaaggcat cagatgatta agagagccaa
     1741 ccatttctgt cactaaaata attgctgtca gcggtgcttt tccaatcgct gtgaaataac
     1801 cagccatggc aataacgaca aaatccttga tgtagattgg atccatcccg aaatcaacca
     1861 taatgtttcc ataaaagcta ccgattaaag cccccaatgt caatatcgga aggaaaatcc
     1921 cccctggaag acccccaccg taagaaatca tggaaaaaac gaatcgaaga acaaacaaac
     1981 cgataatcat aatgattggc aaggaagcat gtccaaaagc taaaacgatt tgattgcctc
     2041 cgcctagagt actaggccat aaataaccaa ttggcaaaat cagcaagaaa ggaacgaacc
     2101 cgtgaaaata agctggtaaa ggcaatcggt tgtaaaattt tggcaaagcc agcaagacat
     2161 tctgataaat aaaacctaag atgcctagaa gtactcctaa gatcactaag gaaccataat
     2221 atttcaacgg caaagaagga aggtcaccta gatataaaac aggtctcaag ccgaaaaagt
     2281 aaagagagac gaagttagca gtgattgctg aagcaaatga tgtcagccaa acaagtggag
     2341 aaaaggagtg atggatttct tctaaaacaa agagtaatcc tgcgatagga gcattgaatg
     2401 ctgctgcaag tccgctactt gcaccgctag aaattaaaat cttttcttct gaagatggac
     2461 tccgaaacaa ttgtccagct ccttgtccaa tggatgcgcc aagctgtata gaaggtcctt
     2521 ctctacctag aaaaaggccc gcaccaactg aaagtacacc gccaatgaac tttttccata
     2581 aaacagagaa ccagttcatt gataattccc cgcgaagctg tccctctact tgtggaatcc
     2641 cgcttccctt gatatccggt tcatttttga ccagataacc cacgaaaaat gctgcaagga
     2701 tgcagcaaat agctaggcca attaagtaaa tcggctgttc ccgactcatt tcatagatac
     2761 ttcgaactaa atcgcttagc ttttcgatag ataatcgaaa caggctaacg acgattcctg
     2821 tcagcagtcc aacaaatatt ccttttagaa taaaatatat ctgagtctta tctaatttct
     2881 tgatttttcc ttttttttcc attcaaacgc ctcgctttct tttaatttag cgcgaaatca
     2941 acgaattgac aaggaaaaat ccataaaaag aaacaaatga ttttgaacta gctagtttaa
     3001 tgaaagccaa gaaaatgcta taattaacaa gattgataaa agaaaggatg ttgttaatgt
     3061 catccaaagc tgaattatta aacgggatga acccccgtca gaaagaagct gttcttcaca
     3121 ctgacggacc attactgcta atggcaggtg caggaagtgg aaagacgaga gtgctgacac
     3181 accgcattgc ctatttgatt gaggaaaaag aagtcaatcc ttggaacatc ctagcgatta
     3241 cttttaccaa taaggctgca aaagaaatga aagaacgtgt caatgcgatt ctcgcatctg
     3301 gaggcgaaga tgtctgggta tcgacttttc actcgatgtg tgtgcgtatt ttaagaagag
     3361 atgttgattt tatcgggtat aaccggaact ttacaatcat cgattcttct gaacagctga
     3421 ccttgatgaa acggatacta aaagaactga acattgatcc gaaaaaatat gatcctcgaa
     3481 gtatattagg aacaatctct caagcaaaaa acagcctgca gacccctcag gattttgcaa
     3541 aaatgcaagg cagttattac gaagagattg cggcaaaatg ttatgctgct taccaaaaag
     3601 agcttcaata taatcaatgt atggactttg acgacctgat catgaatacg attcgattat
     3661 ttgaagaaca tcctgattca ctcacgtatt atcaaaacaa attccattat attcatgtag
     3721 atgagtacca agatacaaat cacgcacagt atacgctagt caatttattg gcaggtagat
     3781 ttaggaacct gtgcgtcgtg ggagatgcag atcaaagtat ttacggctgg cgcggtgcag
     3841 atatgcagaa catcttggat tttgaaaaag attatccaga tgctgcagta attttgctag
     3901 aacaaaacta tcgctccacg aaaaacatcc ttagcgcagc aaaccaagtc attgaaaaca
     3961 atagcaatcg aaaaccaaaa aacttgtgga cagaaaacaa agaaggaaac aagatcactt
     4021 actaccgtgc agataatgaa cgagacgaaa cccgttttat cgtagaccgg atgcaagaag
     4081 agattcgaag caatcatcgg aattacggcg actttgccat cttgtaccgg acgaacgcac
     4141 aatctcgtgt aatggaagaa actctgttga aagcaaatat tccttataaa atggttggag
     4201 ggcacaagtt ctacgatcgt aaagaaatca aagatatttt agcgtatttg aatgtgctgg
     4261 taaatcctca agattcaatc agttttgagc gtatcgttaa ttcgcccaaa cgaggcatcg
     4321 gaccaggatc tatcgaaaaa ctacgcagtt ttgcttctct tcatgaatgg ccactgcttg
     4381 aagcagcgca aaatgtggat ctggcaaata tcggtggaaa agcaggacag caattaggtg
     4441 catttggaga gatgatccaa gaagtcactc agatgatccc atacttgaca gtgacagaat
     4501 tgacgaaaga ggttttggat cgtagcggat atctagaaga cttaaaaatc caaaatacat
     4561 tagaagcaca agcacgcatc gaaaacttgg aggaattttt aacggtcaca caagaattcg
     4621 acaaacaatt cgaacagcaa aatgaagaag acgctgatgc accagaagaa aaactaacgg
     4681 tcttcttaaa tgatctggca cttgtttccg acattgacaa tttagaagaa gatgcttccc
     4741 aagtgacatt gatgactttg catgcagcca aaggattaga atttccagtt gtattcctga
     4801 ttggtctaga agagggggtt tttcctttat ctcgagcatt gatggaagaa agcgaactgg
     4861 aggaagaacg ccgtctcgca tatgtgggga tcacgcgagc agaagaagcg ttgtatttaa
     4921 caaatgcttt ctccagaaca ttatatggac ggacgcaata taaccgacct tctcgttttg
     4981 tagaagaaat cgatcaggag ttactggaaa tcgaaggcat gcgtcctacg cctaaaaaaa
     5041 cgcctgtatt cgctaaaaaa acagcatata gctataaaca gccagagaca gcggttgttc
     5101 cctctaaaag tgctactgga ggggaaaata acagctggaa acctggagat aaagtcaaac
     5161 acaaaaaatg gggtctagga acagtcgtcc gagtaagcgg gacgtcgaag gatctagaac
     5221 tagatgtggc tttcccaagt caaggagtaa aacgtctttt ggctgccttt gcgccaattg
     5281 aaaaagctta gtagtggagg gtaaatgatg gaacatgaaa tgacgattga cgaagctgct
     5341 aaacgagcag aagaacttcg tacaagactg aaccaatggt ctcgcgaata ctacgtagaa
     5401 gataaaccaa cagtggaaga ctatgtttat gataaagaat acgcagaatt agtagcaatc
     5461 gaggaacaat atcctgattt gatcacgtct gattctccca ctcaacgagt aggcggaaaa
     5521 gtattagaag gttttgaaaa agtcactcat gatattccgc tttacagttt gaacgacgtt
     5581 ttcagcaaag aagagctgat agcttttgat caacgtgtac aaaaagcagt gggacgtgta
     5641 gttgactatt gctgtgaact aaaaatagat ggactttccg tttctttgcg ttatgaagat
     5701 ggaaatttcg tgagaggtgc gactcgcggg gatggaactg taggagaaaa catcactgaa
     5761 aacctaaaaa ctgtccggtc ggtcccaatc aaattaaaag aaccaatgaa catcgaagta
     5821 agaggcgaat gtttcatgcc aaaacggtca tttgtccaat tgaaccaaga cagagaagca
     5881 gagggaaaag atatctttgc gaatcctcgg aacgccgcag ctggttcttt gcgtcagtta
     5941 gattctaaaa tcacagcgaa aagaaatctg gacacttttt tatacacagt agctgatttc
     6001 ggtcctatgc aagcaaaaac ccagtatgat gcgctagaag agctagaaaa aattggattc
     6061 cacacgaatc gggaaaaacg tctatgtcat tcgattgatg aagtgtggtc atacattgaa
     6121 gaatatcatg acaaacgtgt ggatttgccc tatgaaatcg atggaatcgt catcaaagtg
     6181 aatgaattct cgctacaaga tcaacttggt tttactatca aagcaccgag atgggcagca
     6241 gcttacaagt ttccgccaga agaagttgaa acgctaatcg aaaacatcga atggacagtc
     6301 ggtcgaacag gggtagtaac gccaacagct atcatgacac cagtccgagt agcaggaacg
     6361 acagtaagcc gtgccagcct gcataacggt gattatatta agttgaagga tatccgcttg
     6421 aaagatacag ttctgattta taaagcggga gacatcatcc cagaagtttc gcaagtcgtg
     6481 ttagataaac gacctaaaga cagcgaagaa taccaattgc ctacacattg tccagtttgt
     6541 ggaagtgaac ttgtccattt ggatgaagaa gtagccttac ggtgcatcaa tcctaaatgc
     6601 cctgctcaaa tgaaagaagg tttgaaccac tttgtttcaa gaaatgcgat gaatatcgat
     6661 gggttggggc cgcgcgtact agaacagatg tatgataaaa aactagtagc agatgttgct
     6721 gatctttaca aattgacaga agaagagtta ttgacgttgg acaaaataaa agaaaaatca
     6781 gcaaataata ttttgacagc aatcgacaac agcaaagata attcggtcga gcggttgatt
     6841 ttcgggttag gaatccgaca tgtcggagcg aaagcagcaa aaattttagc agaacatttc
     6901 ggtgatctag aaacattgag taaaagtgac tatgagtcga ttattgcgct tgatacgatc
     6961 ggtgatatca ttgccgacag tgtagtcact tatttcagta acgaagaagt ccatgaattg
     7021 atgaatgaat taaaacaagc aggcgtcaat ttcgaataca aagggcttcg aaatgcccaa
     7081 ttgcaagaag tcgagtctcc ttttaaagaa aaaacagtag tccttactgg taaactaaca
     7141 cgatttactc gtgaagaagc aaaagagacg atcgaaaatc ttggcgggaa agtgactggt
     7201 agcgtatcga aaaaaacgga tatcgtggta gcaggagaag atgctggcag caagctgacc
     7261 aaagcacaag aattaggaat cgaagtttgg acagaagatc aaatggcaga tgcattggct
     7321 aaaagtcgct cagtagaaga atgaaaaatg agttttttca gacatatttc aataaacaat
     7381 ttcagacacg gcactcttgt gttaaaatag agagcaaagt ttttaactcg tttagaaaga
     7441 aggggtagta tggcgattag tgaagaacaa gtgaaacacg ttgccaaatt agcaaaactt
     7501 gcgttcgcag atgaagaact tgcaagtttt accgaccaat taggtaaaat catcgatatg
     7561 gttgaaatgc tggaagaagt agatacggaa ggtgttccat ttacgtcgaa tgtggttgaa
     7621 acagtaaacg tgatgcgaca agatcgggcg caagctggat ggaaccgcga tgaattattg
     7681 aaaaatgtgc cggaaacaga agacggcttt atcaaagtac ccgcaatcat cgataatggg
     7741 gaggctggcg cataatgaca gaattacaca atcgttctct agaagaactg catcatatgc
     7801 ttgtatcgaa agaaatcact gtacaagatt tgacaaaaac aacatttgaa cgaatcaaag
     7861 aaacagagcc tgaggtcgag gcattcatta cattgaacga agaaaaagca ttggaacagg
     7921 caaaagcttt agatgaaaaa gggattgatg aaagcaacgt ccttgctggt attccaattg
     7981 gtatcaagga taatatcgta acaaaagata tcttgacaac cgctgcatct aagatccttt
     8041 acaattttga accaatttat gatgcaacgg tcatggataa aatctatcaa gcagatatga
     8101 tccctgttgg taagctaaac atggatgaat ttgctatggg tggaagtaca gaaacttctt
     8161 atttcaagaa aacaaaaaat gcttgggacc caacaaaagt acctggtggc tcttctggtg
     8221 gttctgcagc agcagttgca gctggacaaa ttccagtttc tttaggtagt gatactgggg
     8281 gaagtatccg ccaaccagca gcttttaacg gcatcgtagg gatgaaacct acttatggcc
     8341 gtgtctcacg ttttggtttg attgcctttg cttcatctct tgaccaaatc ggaccaatga
     8401 cccgtacggt gaaagataat gcattagcat tgacagctat cagcggttat gacgaaaaag
     8461 acggtacttc ttcaggcgtt tcagttcctg attttgcaga gggactgaca ggcgacatca
     8521 aaggaatgaa gattgcttta cctaaagaat acttaggcga aggtgtggcc cctggcgtaa
     8581 aagaagctgt attgaaagca gcagaaacat tccgttcatt aggtgctgtt gtggaagaag
     8641 tcagcttgcc tcattcaaaa tacggtgtgg ctgtttatta tatcattgcc tcatcagaag
     8701 caagttccaa tttgcaacga ttcgatggta tccgctacgg ttatcgctct gaaaatgtga
     8761 aatcattaga tgatgtatat gtcaactcac gttcagaagg attcggtgaa gaagtaaaac
     8821 gccgtatcat gttaggtaca ttctccttaa gcgcgggtta ttatgacgct tacttcaaaa
     8881 aagcaggaca agtccgtaca ttgatcaaac gagattttga aaaagtcttt gcagattatg
     8941 acttgatcat cgggccatct tcaccaacag ttgcattcgg aatcggtgag aacattaacg
     9001 atccaatcac aatgtacatg agtgacatct taacgatccc tgtcaacttg gctggacttc
     9061 caggtatgtc tgtaccggca ggtttatcag aaggattacc agttggattg cagatcatcg
     9121 gtaattattt tgatgaaaaa acgatgtatc aagcggcata tgcgtttgaa caagcaacag
     9181 aattccataa acaacaacca gctattttag gagggaaaga cgaatgaatt ttgaaacagt
     9241 cattggacta gaagtccacg tcgagttaaa aaccaactca aaaattttct ctccagcgcc
     9301 agctcatttt ggtgcagaac caaacagcaa tacaaacgtg atcgactggg gttatccagg
     9361 cgtgctgcca gtaatgaata aagctgcgct ggaattcggg atgaaagcag ctttggcatt
     9421 aaactgtgaa atctctaaag atactcattt tgaccggaaa aattactttt acccagataa
     9481 tccaaaagca tatcaaattt cccaatttga tcaaccaatc ggtcatgacg gatggatcga
     9541 aatcgaagta gaaggtaaaa agaaaaaaat ccggatcgaa cgtgtccatt tagaagaaga
     9601 tgctggaaaa aacatgcatg gtattggcgg ttattcttac gttgatttga accgacaagg
     9661 cacgcctttg atcgaaatcg tttctgaagc tgatatgcgt tcaccagaag aagcatacgc
     9721 ttatctagaa gcattgcgat caattatcca atttacagaa gtcagcgatg tgaaaatgga
     9781 agaaggctct atgcgctgtg atgccaatat ttctcttcgc ccttatggac aagaagagtt
     9841 cggtacgaaa gcagaattga aaaacttgaa ctctttatct ttcgtgaaaa aaggtttagc
     9901 ttttgaagaa aaacgtcaag caaaagttct tctttcagga ggagaaatcc aacaagaaac
     9961 acgccgtttt gacgaaacaa cgaataaaac agttttgatg cgtgtcaaag aagggtctag
    10021 tgattatcgt tacttcccag agcctgatat cccgaaattc gtgatcgatg acgaatggat
    10081 tcaaaaagta aaagctagtt tgcctgagat gccagcttct cgtcgcgaac gctatatccg
    10141 tgagtttgga cttccagagt atgacgcaat ggtattgaca cttacaaaag aaatgtctga
    10201 tttttttgaa gctgttttgg aaaatggtgc agatgccaag caagcatcta actggttgat
    10261 gggtgaagtt tctgcttact tgaacagtga gaaactggaa ttagctgaga caaaattgac
    10321 acctgagaac ttagcaggaa tgattcgttt gatcgccgat ggaacgatca gctcaaaaat
    10381 cgccaaaaaa gtcttccgtg aattgatcac aaatggcgga gaagcccaag aagtagttga
    10441 aaaaaatggt tgggtgcaat tatctgatcc gaacaaacta ctgccaatca ttaatgaaat
    10501 tttggataac aaccaacaat ctgtcgatga tttcaaaaat ggaaaagatc gtgcggttgg
    10561 cttcctagtc ggtcaaatca tgaaagcaac taaaggacaa gcaaatcctg gtgtcgtgaa
    10621 taagctactt aaagatgaat tagcgaaacg atagaggctg aaaatgaaaa aagcacgcgt
    10681 aatttataac cccacctcgg gaaaagaatt attgaaaaaa aatttggcgg atatcctaca
    10741 ggcattagaa gaagcaggat ttgaagccag cgcctatgca actacaccag aaccagattc
    10801 agccaagaat gaagcatgta gagtcgcaga actaggtttt gatctagtag tggcagctgg
    10861 tggcgatggc acaatcaacg aagtggttaa tggaatcgct ccgcttgaac accgtcctaa
    10921 aatggccatc atcccagctg gtacaacgaa cgactatgct cgagcattaa aaattcctag
    10981 agataatatt aaagcagcag cagaagtgat aaaaaaacac caaaccgtaa aaatggacat
    11041 cggcctttct gataacagtt acttcatcaa tatcgctgca ggtggctatt tgacagaatt
    11101 gacttatgaa gttccatcag aactaaagag tatttttggt tatcttgctt atcttgcgaa
    11161 aggagcagag atgctccctc gagtaaagcc aatcaaaatg cgcttgaagt atgatgaagg
    11221 ggaatatatc ggaaatgctt cgatgttttt cctagggctg acgaactctg ttggaggatt
    11281 cgaacagatc gcgccagatg ccaaattgga tgatgggaag ttttctttaa tcattgtgaa
    11341 gacagcaaat atctttgaga tcttgcatat tgcagcactc atgctgaatg gcggtcgaca
    11401 tgtcgatgat ccaagaataa tctataccaa aacaagcagt ttgttcgttg aaacagaaga
    11461 tgatatcaat cgaccagtaa tgatcaactt agatggtgaa tacggcgggg aagcaccgat
    11521 gcatttccaa aatcttcatc aacacattga gttctttgcc aataccgatc agattcctga
    11581 tgaagcaatc acaggtacag atgaagaaga attagaggtt gtcagcaaag aattcgtaaa
    11641 agaagtcgaa cgtttaacgg atgaagatat cgacggtgac ggaaaaatag cagacgaaac
    11701 acctaatcaa aagaattaat caaaagccgg tgctgaacgt agtaaggtca gtgccggttt
    11761 tagttgtgta attagctgat ttatagtaga gttcagtagg attgaaaaat aggcttaaag
    11821 gagcaaaata tgacagaaca gcaaatagta aagaaaaacg aacgatatac agtcgaaatc
    11881 gtcgacttga gttatgaagg aatgggggta gcaaaaatcg atggttatcc attatttatc
    11941 gaaaatgcac tccctggaga agaagtagaa atactagtag taaaagcagg caataagttt
    12001 ggatatggaa aagtcgaaac atttattaca caatcacctg atcgtcagcc tgtagaaaac
    12061 aatgcattac ttcgcatagg tatcgcacct ttagcccatt tgaagtatga acaacagctt
    12121 gtcttcaaac aaaaacaagt agagcatgta atggcgaaga ttgctaagat gccagaagtt
    12181 cctgtacttc caacggtcgg aatggcagaa ccattcggct accgtaataa agcacagatc
    12241 cctgttcgcc gtatcgatgg acagttagcc accggttttt acaaaaagaa tagccatgat
    12301 ctagtcccta tcgaagattt ttatatccaa gatcctgtca tcgatcaagc aatcaaagta
    12361 gtcagagcta tcttgcaacg gttccaagta cgaggatata atgaagaaaa aaatgaagga
    12421 cagcttcgtc acatcattat ccgtcgtggt cattattccc atgaaatgat ggtcgtttta
    12481 gttactcgta aagaaaaatt ctttaaatcc aaggaaattg ctgaagctat tcatgaagaa
    12541 ttgccagaag tggtttcggt catccaaaat atcaatgaag aaaaaacaaa tgtgatttta
    12601 ggagacaaag aaaaagtttt atatggcaga gactatatcg aagatcagct attaggaaaa
    12661 acctatcgaa tctcagcaaa gtcattttat caagtaaata ctcaacaagc agagtatctg
    12721 taccaaacag ccatcgagtt tgccgagtta gataaaaaag acattgtgat cgacgcttat
    12781 tcaggaatag gtacgattgg cttgtcttta gcagacagag tggataaggt gtatgggatg
    12841 gaagttattc cagaagcaat tgaagatgca cagttcaatg ctttaaataa taaaatcgaa
    12901 aatgttcatt atgaagtcgg aaaagcagaa aaagttatga aaaaatggca agaaaaaggg
    12961 atcaaaccat ctgtgatcgt agttgaccct ccacgaaaag gattagatca tcattttatt
    13021 gaatcagcaa tcgaaatgtc accagaacga atcgtctata tctcatgtaa tccggcaact
    13081 tttgcaagag atgctaagtt atttgcagaa gcaggttatg ccgtgaagaa agttcagcct
    13141 gtagatcttt tcccgcaaac ccatcatatc gaattagtcg cttcattcgt taaaggagaa
    13201 taaaaatgga ccaaaaagaa aaaatgaaag aattgatcaa aaagaaacaa ggtggaggcc
    13261 gatccaaaca aatggaaaca cctaagaatg atcgtaaaaa catgagaaaa ggccctaaaa
    13321 tctttaataa atagacgtgc taaagaaaga ctaagaagtg ttaacttttg ttattgtttc
    13381 ttagtctttt tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg
    13441 tgcaaaatca aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca
    13501 atttgattaa ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac
    13561 taaatataca tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga
    13621 cgacgtagca gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg
    13681 atagtgaacg tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg
    13741 tcttagattt tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac
    13801 cggatctacg aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt
    13861 ggatttgctt caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt
    13921 ttgtcaaaag cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg
    13981 ataggaaata acactatggt ataatcagaa tgcttaatac taactactga gggagcctta
    14041 tgaaaaagaa aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt
    14101 ttgcaatctt tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata
    14161 tgttttcaaa tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg
    14221 tggcgaaaaa aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct
    14281 actatccaaa aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat
    14341 tcgtcggcgg agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca
    14401 acatcgcagt ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag
    14461 ttcttcaagt gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc
    14521 tagacatgga acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat
    14581 atgcagctat acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc
    14641 caaaagatag tctgaaagga accatttctt attgcggacc tgttgatttg aagctaacag
    14701 cacagcagca ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt
    14761 tgattggaac aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc
    14821 atgtttctaa ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg
    14881 agcaaggtca agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt
    14941 tttatacaga tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag
    15001 aatcgcaaaa ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg
    15061 tgaaaaagcc gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt
    15121 caataccttt ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc
    15181 aaaatgattg gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct
    15241 tgcccaattg atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt
    15301 gtctcgatat tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt
    15361 ttcaatgccc gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga
    15421 agaggggatt cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag
    15481 ccacctagat gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag
    15541 gcacgcatcc tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact
    15601 ttatatcccc cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg
    15661 gttttagtca tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg
    15721 ttatccacga cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta
    15781 acagtcccaa caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta
    15841 tcagatagat tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc
    15901 cctactcgca aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag
    15961 atcacaccaa tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc
    16021 aacgcagcag ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga
    16081 tcgttattca acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct
    16141 ctaattccaa ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt
    16201 gtttagtaaa cagtatacag gaattataaa aaaagtaaac ggtttccgtt ttgttgggag
    16261 tgaaatgatg gatattacgc atagaaaaac agataaaaat ttcatcttat ttgatgaaaa
    16321 gagccgagtc ttccatttac gaaatacatt tctttcttat ctgataaaga tagaagaaag
    16381 caatgtattg gcgcatattt attttggcaa accagtcaag cagtacaaag acaataaaaa
    16441 ttatccccga agagatcgtg gattttcagg aaatgttccc ttaaatcctg accggtctct
    16501 gtcgaaagat acactaccgc aagaatactc ttctcatggc agcatggact ttcgaacgcc
    16561 cgcatcgatc attcagcgca agaatggatc agacttactg gatctgagat atgacagcca
    16621 ttatattaca gatgggaagc cagagatcga aggcttgcct caaacttatg tgatggacaa
    16681 gtcagaggca caaacgttgg tgatttcact taaggataga gaaacagcga tttatttcga
    16741 tttgttctat accatattta ctgatagagc agtgatcaca cgatctgtaa agataagaaa
    16801 tgaaaccgga gaaacaatca aactagaaaa agcggctagt ttccagctag actttgccca
    16861 tactaggcga tttgatgaag tcattgcttt gccaggagca catgtcaatg aaagacagat
    16921 ttcacggcaa tctgttttat ctggaacaaa agtatttgaa agccgtcgag gaacgagcag
    16981 tcaccatatg aataacttta ttgctttagt tcatcatcat acgacagaaa atactggaga
    17041 agcgatcgga cttcaatttg tttattccgg caaccattct ttcgaacttg aaaaagatca
    17101 gatcaatcaa ttgagagtcg tcggtgggat caacagccat cgtttttcgt gggaattgaa
    17161 tgcaggccag tcttttcaaa cacctgaaat gatcctttct tactcaagtc agggattgaa
    17221 caaaatgagt cagatccacc atgagttgct acgtgaaaga atcgcaagag gtcgtcatca
    17281 atttgctgaa cggccgattc tggtcaataa ctgggaagcc acttactttg acttcaatag
    17341 tgaaaaaatc aaggcgatca ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt
    17401 agatgatggc tggtttggaa aaagagatgc ggacaattct tcattaggag actggttcga
    17461 gtatgaaggc aagttgacaa atggattacg tgaaatagca gattatgctc atagcaaagg
    17521 attgaaattt ggtttatggt ttgaacctga aatgatctcg gttgattctg aactgtatcg
    17581 aacacatcct gattttctta tgcaggagcc aggtagaatg ccttctgctt ccagatcaca
    17641 acatgtactg gattttacaa ggttagatgt tcggcagacg attgagaaac aaatgagaaa
    17701 gattttagat acgattcctc ttgactacat caaatgggat atgaaccgtt cgctttcaga
    17761 tgtctattcg attacccttg acccacagag acaaggagaa gtagcgcatc gttatatgct
    17821 cgggctttat gaactacttg aacatttgac aactgattat ccggaaatct tatgggaagg
    17881 ctgttctggt ggaggtggac ggtttgatgc cgggtttatt tattatatgc cgcagtcatg
    17941 gacaagtgat aacacggatg cagtggagcg tatgaaaatc caatatggaa caagccttgc
    18001 ttatccgatt tcgtccatca cagctcatgt atcagctgta ccaaatcatc agaccgacag
    18061 atctacttcg ttgaaaacaa gaggagaaac agctatgagc gctgtttttg gttatgaact
    18121 ggatttgaca aagctgtctc ctgaagaaaa aaagcaagta aaagaccaaa ttatatccta
    18181 tcaaacgatt cgtccggtga tccagtatgg ccattattat cgtttggcga gtccttttga
    18241 agaaaatatt gccgcatgga tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt
    18301 agggaggata ctagcttcag ctcaaccagc gttccatgaa gtatatctga tggggttaga
    18361 tgatgaggca ctttatcagg aacagacctc gaagcggata ttttcggggg ccgaattgat
    18421 gacagttgga ctttacttcc ccgattttca aggtgatttc caaacagaac tgcttcattt
    18481 caaaaagtta tgagagagaa ggaaaaaagt atgaaagcaa atataaaatg gttagatgat
    18541 ccacaagttt ttagagtgaa ccaatcaaat gcacatagcg atcatgtttg ttatgcaaca
    18601 agggaagaag tagaaaccac atcctccctt aaacagtctt taaatggatc atggcgtttt
    18661 tgttattcgc caaatccttc ggtacgacca aaaacatttt ataaaaaaga ttttgatgcc
    18721 tgttcttttg atgaaatcac tgttcctagt catattgaat tagcaaatta tgacaagatc
    18781 cattacatca atacgatgta tccgtgggag ggacatgtat ttcgccgtcc ccctttcacg
    18841 acaagccctg agaaagaggc ggaagggatc ttttcagctg ctaaagacaa tccggttggt
    18901 tcgtatttga cgacttttcg attagaagaa ggtctaaagg gaaaacaaat ctcgattcat
    18961 tttgctggtg tagaacaagc aatgtttatt tggttgaatg gtcattttat cgggtatgcc
    19021 gaagattcgt tcactccgtc tgaatttgat ttaacacctt atttggaaga agaaaatatt
    19081 ctagctgtgg aggtttacaa aagaagtaca gctgcattct tggaagatca ggactttttc
    19141 cgattttttg ggatcttcag agatgtcttc ttagttgctc gaggacaaaa ccatttagaa
    19201 gatctttgga tcaaacctcg cttagatgac aaatgtcaaa atggcaaact tgaagtagat
    19261 ttgtcttttg agcaagtagc caaaaacagt cagctgaatg tagcgatact agatcctgat
    19321 gggaaggaaa tctatcaaca gcaatatccg ttaaaagagc aaatgacgat tcgcatcgat
    19381 ggatttgaaa atgtggcact gtgggggcat tttcagccca atttatataa gcttgaattg
    19441 gagacgatag acgaagaaaa aagagtaacg gagtatattg actattcctt cggttttcgt
    19501 aaaatcgaaa tcaaagataa aatcatctac ttcaatggaa aacggatgat tatcaacggg
    19561 gtaaatcgac atgagtggca tccgaaaaaa ggacgcgcca tcactataga agatatggaa
    19621 aaagatttgg ctatcataaa aaatgcaaag atcaacggcg tacggacatc tcattacccc
    19681 aaccagatca cttggtatta tttatgcgac caagcaggaa tccatgtgat ggcggaaacg
    19741 aacctcgaat cccatggctc atggcaaaaa atgggcgcat ttgagccttc ttacaacgta
    19801 ccaggtgcag tacctcagtg gcaggcggct gttcttgatc gagcaaaaac gaatttcgaa
    19861 acattaaaaa accatccaag tattcttttt tggtctcttg gaaatgaatc ttatgcggaa
    19921 gaaaacattc gacaaatgaa cgattacttc aaaaaagtag accccgatcg acttgtccat
    19981 tatgaaggga attttccaaa tccagcattc gaagattcga tttcagatgt aaaaagctgc
    20041 atgtatgcgc caccagaaga gatcattgac taccttgaaa ataaagcgga aaaaccgttc
    20101 atcttatgtg aatatatgca tgacatgggg aattcgttag gtggaatgga ttcttacatg
    20161 acgttgcttg atcgttatga aatgtaccaa ggaggctata tatgggattt taaagatcag
    20221 gcgatccaag tgactgatcc aatcacaggc agagaagtga tgcgctatgg cggtgacttt
    20281 gacgataaac caagtgacta tgaattctca gggaatggga tcgtttttgc agatggacaa
    20341 gaaaaacccg ccatgcagga ggtaagatat tattatgaaa aatacagtaa ataaaagtca
    20401 tatggatacg gaaaaagttg caatcgtctt cggcgactgt acattaggtg tcaaatcggg
    20461 gaatacgcat tatatttttt cttatacaag aggcggactg gaatcgctca ataaaaacgg
    20521 aaaagaatgg ctataccggg aaacacttcc agttttttgg cgagcaacga ccgataatga
    20581 cagaggtagc ggatttggat ttaaatccgg tatgtggtta ggagccggat tgtatccaaa
    20641 agtcaaagaa atagaagtac tggtggacaa taaaaagatc gatttgccga ttgctccagt
    20701 aaataatcaa tattcaagta atgaatacgc tgaaacggtt gaaatcattt ttacactgga
    20761 aatcaacaca gtaccaaaaa cccaagtgac aatttcctac caaatcgctg gagagggtaa
    20821 aatgcttgtc aatatgcatt attatggaca aaaaggatta ccggaattac ccgcattggg
    20881 tttaaggttc attatgccaa cgaaagcaaa attttttgag tatgaaggcc tatcagggga
    20941 aacatatccg gatcggatgg ctggtggtgt cgaaggtatt tataaagtgg caggactccc
    21001 cgtttcccca taccttgttc cccaagaatg cggcatgcat atggaaacga aatgggtaga
    21061 actaacaaga aatcaaacgt tggataatac ggataaagat caagaagatt tttcgttgcg
    21121 atttgaacag caggatacgc catttgcttt ttccgcttta ccattcaaag cagaagaact
    21181 ggaacaggca acacatatag aagaactacc gccagaaaga cgaacggttc ttacgatttt
    21241 tgcaaaagta agaggagtcg ggggtatcaa tagctgggga gcggatgttg agtcacctta
    21301 ccgaatcagc agcgaagagg accatgaaat cggctttatt attcagtagc aatctttaga
    21361 ttattatttg aaaaacggag tttacaagac aaagctgctt tattctatat gtcatgaaca
    21421 agagagtaaa aaagttctca gatacttttg ttcatgacta ttttcttcaa taaaaccaat
    21481 tgttattttt ctgaaaaaca aagttaacta tcagaaaatt aaaaaaagtt tcataaaaat
    21541 gattgacagt cgattttttt atgtctatac taggtacaat tcttacatca gtttggagcg
    21601 attttttatg aaatctagaa aacttacaaa actgaatacg tattacttta gctattttag
    21661 ataggtttgt attcatgact tcgtgggtac aaacaaacca ggtttgtatc tatgatggct
    21721 aaagattttt aaacacgact tacagccacg tagagaaatt catctatagt ggctatttta
    21781 tatttttgca gcgcttttta aggctcgttg aagatgttca ctgtagaggc aatttcctac
    21841 agggacatct ttttttgttg attatctggg tcataagtag gaaagaagga gcgaaaatga
    21901 gaacaacagg acacggtcat ttagtaggaa ttaatgtaaa accaccttga aaaagtagag
    21961 aaaatcagcc aagatagata agtaaacaaa agaaagcggg aaaaacaatg atagtaatta
    22021 tgaaaaaaga agcaacggaa gaacaaataa aaaaagtcgt cgatcatatc gaaaaagaag
    22081 gattcaaagt ctcaatcgat caaggaacag aacgagtagt gattggacta aaaggtgata
    22141 cgcgaagttt acaagaagga gcatttacac gctatgaagg agtagaaaat gctgtgcgta
    22201 ttttaaacac atacaaattg acttctcgag aattccatcc tggcaataca gtcgtggatg
    22261 tagacggagt aaaaattggt gacggcagtt tcgtgacgat ggctggtcct tgttctatcg
    22321 aaggattaga ccaaatcaga gaatgtgcgc gaatggccaa agccgggggc gcaaaaattc
    22381 tgcgaggtgg tgccttcaaa ccacgtactt ctccgtatgc tttccaaggt ttagaagaag
    22441 aaggattgaa atacatccgt caagcggctg atgaatttgg aatgaaagtg attactgaag
    22501 tgatggatga agggcatatc gacatggtgg ctgagtacag cgatatccta cagattggcg
    22561 cacgtaatat gcaaaatttt aaactgctat ctgctgtcgg taaaacaggt aaacctgttg
    22621 gattgaaacg tggaatttcc ggtacgatca atgaatggct taatgctgca gaatacattg
    22681 cagtcgaaga caaatctcct gtcattttta tcgaacgtgg gattcggact tacgaaacag
    22741 cgacacgtaa tacgtttgat ttaagtgctg tcccattgat gaaaaaatta actcatttcc
    22801 ctgtgattgt tgatccaagt catggtacag gaatttggga acttgtacca ccaatggctc
    22861 gtgcgggtgt ggcaagcgga gcagatggca tgatcgtaga gatccatcct gatcctgcaa
    22921 atgcttggtc agacggtccc caatcgttga atgaaaagac ttactcacgc atgatgaaag
    22981 aagttgcgat catgaaagaa gcaatggaaa agattcagac actcgattaa ttccagcttt
    23041 ttaaaagaaa gaagtgaaat gaatgcttac tgtacatgtt gataaccaaa aatacgatat
    23101 tcatattgaa aaaggcgcac tagaacaggc tggatcatgg cttcaaacga tttggagacc
    23161 tcaaaaagta gctgtgatca ctgatacaaa cgttgctcca ctatatgcag aaaaaatcat
    23221 gttccaattg agacaagcta attttgaacc agtcctttca atcgtaccgg cgggtgagac
    23281 cagtaaatct ttagaacaag cagctgagtt atacgatttt ttagcagata atgattttac
    23341 ccgcagcgat gggatacttg cacttggtgg aggaatggtg ggtgatctag ccgcattcgt
    23401 ggcctcgacg ttcatgcgtg ggatccattt cgttcaaatt ccgactacgc tacttgcaca
    23461 agtagattct agtattggcg ggaagacagc agtcaataca aacaaagcga aaaaccttgt
    23521 cggaacattt agccagccag ctggtgtatt gattgatcct gaggttctaa aaactttgcc
    23581 ggtacgtcgt ttacgagaag gaatcgctga aatcatcaaa tcagcagcta tagcagatgc
    23641 aacgctttgg aaacagctga acgagctgaa agacgaaaaa aatcttctgg aacatgctgt
    23701 tccaatcatt actgctacgt tggaaatcaa acgaaaagta gtggaagaag atgtctttga
    23761 ccaaggaaat cgacttattt taaattttgg tcatacaatt ggccatgcaa ttgaaaatac
    23821 agcaggatat ggggtagtca gtcatggaga aggagtagct attggaatgg tagccatcac
    23881 ttctcatgca gaaaaaatcg gcttgtctcc aactggatcg acagctgctt tagaaaggat
    23941 acttcaaaaa ttccatctgc cgattacgta tgatctaagt catccagatg aattattcca
    24001 cgcgataacc catgataaga aagcaagagg aagccaattg aatatcatct tgctagaaaa
    24061 gattgggcaa gcaaaaatcg ttcctgttcc cacagagaca ttcaaagatt acttaggagg
    24121 actgtaagat gcgttatatg actgcgggag aatcccatgg accagaatta acagcgatta
    24181 ttgaaggact accagcagga ttacctctgt cagaagaaga aatcaatcac gaactcgctc
    24241 gcagacaagc tggttacggc agaggaggaa ggatgaagat cgaaaaagat cgtgttcgga
    24301 tcacgtctgg catccgtcac ggcaaaacaa ttggttcacc tgtcacttta gtggtagaaa
    24361 ataaagattg gaaaaattgg acaactgtca tgtctgtagc agaagtgcct gaaaaagacc
    24421 aatcactgcg gcgagttgca cgtccgcgtc caggacacgc ggatttggta ggcggtatga
    24481 aataccatca tcgagatctt cggaatgtgc ttgaacggtc ctctgcaaga gagacgacca
    24541 tgcgtgtagc aatcggcgca attgccaaga aaattctgga agaacttgac atcatcgtag
    24601 caggacacgt gatttgtctt ggtggaatcc aagcagaaat acctggaaat atctcgattc
    24661 aagaaatcaa agaaatttct gaagcgtcag aagtacgagt tgttgatcca gcagttgagc
    24721 aaagaataaa agatttgatc gatcagacaa agaaaaatgg agatacgatc ggaggaattg
    24781 tagaagtgct cgttggaggt gttccagctg gattaggaag ctacgtacaa tgggacacga
    24841 agctagatgc caaaatcgca caagctgtgg ttagtatcaa tgcttttaaa ggcgtagaat
    24901 ttggggtcgg attcacttct ggttcattac caggctcaga agtgatggat gagatcctat
    24961 gggaagaaga aagaggctat ttccgacgta ccaataatct aggcggcttc gaaggcggaa
    25021 tgaccaatgg cgaaccactt gtcattcgcg gggtcatgaa acccatccct acattgtaca
    25081 agccacttca aagtgttgat atcgatacaa aagaacctta caaagcaagt gtagaacgtt
    25141 cggatagtac agcagttcct gcagctagcg tcgttgcgga aaatgttgta gccactgtgg
    25201 tagcgacaga gctattgtca aaattcccaa gtgattcgct tgttgagttg aaagttgcag
    25261 tgaaggagta ccgtgactac ttgaaacatt attaaaaagg gggcaaataa gatggcacaa
    25321 catgttttta tcgtggggct tggactgatt ggtgcttcta tggcactttg tattcgtcag
    25381 accaatccag aactgatagt agttggttgg gatagtcagt caaaaacccg tgagcaagca
    25441 atagaacagc agatcgttga ttacgttgca aatgattttg aaacaggtgc agaacaagca
    25501 gacgttatct tattagccgt tcccgtccgt acaaccttag cttatcttgc tgtccttgaa
    25561 agaatcaact tgtctgatca cgtcatcatt acggatgtca gcagtacgaa gcagcaagtc
    25621 gtgacatccg ctgagcagaa aaatcttcgg tttgtcggtg gacatccaat ggctggctct
    25681 cataaatcag gtatacaggc agcagatgat aaactatttg aaaatgccta ttacatcttt
    25741 acacctttgg aaaagacgaa aaaagatgtt gaaaaactgc aagaactatt ttgcggaacc
    25801 aaagcaaaat acgtaactct tacagcaaga gaacatgacc gtataacagg aatgctaagt
    25861 cattttccac atattctggc agctggctta gtcaatcagg cagaacaatt caatcaagag
    25921 tatccgcgag ccaaacaact ggcagcagga ggctttcgag atatcactag gatcgcttcc
    25981 tcggatcctg ttatgtggac agatattttg ttgagcaata agcagatatt attggaacga
    26041 ttggcggatt ggcagcaaga aatggcacaa atagcagaat ggatcatgac tgagaaccaa
    26101 tcggaaatct tttctttttt caatcgggca aaagaaagtc gagaccagtt gcctatccat
    26161 aagcaaggcg cgattcctgc cttctttgat ttatttatcg atgtgccaga tgaacctggc
    26221 gttattgcag aagtaactgg actgatcgga aaagcgggag tttctttgat taatttgaag
    26281 atccaagaga ccagggaaga tattttagga atactgcaaa tcagttttaa aaaccaacag
    26341 gatttgttac aagcaaaaag aatcattatg tctaatacca gttatcattg ctggatcaaa
    26401 taggaggctg gataaatgca attattacaa caaatacatg gattaagagg aactgttagg
    26461 ataccagcag acaaatcaat ttctcatcgc agcatcatgt ttggggcaat tgcggaagga
    26521 acgacgacta tacaaaattt tttgcgcgca gaagattgtc tgagtacttt acatgccttc
    26581 cagcaattag gcgttgaaat cgaagaagag gaagagatga tcaagattca tggtcgcggt
    26641 agccactcat ttgtccaacc aaccgaatca atcgacatgg gaaactccgg tacgaccata
    26701 cgtttattga tgggtatttt ggctggacag ccttttatga ctactctgtt cggggatgct
    26761 tcgttgtcta aacgtccaat ggggcgagtg atggaacctt tacgcgagat gggcgctgac
    26821 ttgcaaggaa aagagaatga gcagtatcta ccaatcactg tgacaggaac caactcttta
    26881 tcacctatcc gatacaatat gcctgtagcc agtgcgcaag tcaaatctgc tttgctgttt
    26941 gcggcattac aagcagaagg cacatccgta atcgttgaaa aagaacgttc ccgtaaccat
    27001 acggaagaaa tgattcgtca atttggcgga aggatctcag tagaaggtaa aacaatcacg
    27061 gtaacaggac cacaaaaatt aactggtcaa cagataactg ttccaggcga tatttcatcg
    27121 gctgctttct ttctagcagc agggcttctt gttccggaaa gccaactgtt attaaaaaat
    27181 gtcggggtca atccaacaag aaccggtatc ttagatgtgc tggaggagat gggcgcagcg
    27241 attacccaga cgaatcacaa tgaacagaac caatcggctg atttaagtgt caagacgagt
    27301 catttgaaga aagcaacgat tggtggagaa atcattccac gtctgattga tgaattgccc
    27361 attctagctt tagtggctac tcaagcagaa ggaatcacga tcatcaaaga cgcagaagaa
    27421 ttaaaagtaa aagaaaccaa cagaattgat gcggtggcag aagaattaca gaaaatggga
    27481 gcaaagataa aagcgacagc agacggtctg atcatccatg ggccgacacc tctgcatggc
    27541 gcaaaagtgt ccagcagagg cgatcatcga atcggtatga tgctgcaagt agctgctttg
    27601 atagctgatg gaccttgcga actagaagga gctgaagctg tttcaatctc ttatccggca
    27661 ttttttgacg atttagcaga acttgtgtca ggaggagaag ctcatggcta gcattttgct
    27721 gattggtttc atgggcgctg gaaaaacaac aatcggtaaa gggttagctc aacgtttaca
    27781 aaaagagtat gtagatttag atacaaagat tgaggaacat atcc
//
LOCUS       DACCSK010000029        26651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCSK010000029 DACCSK010000000
VERSION     DACCSK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.029"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(26..328)
                     /locus_tag="IVP39_001053"
     CDS             complement(26..328)
                     /locus_tag="IVP39_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7586137.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IVP39_001054"
     CDS             complement(353..2446)
                     /locus_tag="IVP39_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7586138.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IVP39_001055"
     CDS             complement(2935..3699)
                     /locus_tag="IVP39_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7586139.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IVP39_001056"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IVP39_001056"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP7586140.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IVP39_001057"
     CDS             complement(4704..5780)
                     /locus_tag="IVP39_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP7586141.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IVP39_001058"
     CDS             complement(5804..6397)
                     /locus_tag="IVP39_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP7586142.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IVP39_001059"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IVP39_001059"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP7586143.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IVP39_001060"
     CDS             complement(8067..8366)
                     /locus_tag="IVP39_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP7586144.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..8878)
                     /locus_tag="IVP39_001061"
                     /pseudo
     CDS             complement(8468..8878)
                     /locus_tag="IVP39_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            complement(8878..10533)
                     /locus_tag="IVP39_001062"
                     /pseudo
     CDS             complement(8878..10533)
                     /locus_tag="IVP39_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10520..12157
                     /locus_tag="IVP39_001063"
     CDS             10520..12157
                     /locus_tag="IVP39_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7586145.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLGYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATALTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPENAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(12302..13042)
                     /gene="truA"
                     /locus_tag="IVP39_001064"
     CDS             complement(12302..13042)
                     /gene="truA"
                     /locus_tag="IVP39_001064"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7586146.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEVPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(13233..13868)
                     /locus_tag="IVP39_001065"
     CDS             complement(13233..13868)
                     /locus_tag="IVP39_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7586147.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(14015..15256)
                     /gene="sstT"
                     /locus_tag="IVP39_001066"
     CDS             complement(14015..15256)
                     /gene="sstT"
                     /locus_tag="IVP39_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7586148.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            16465..18483
                     /locus_tag="IVP39_001067"
     CDS             16465..18483
                     /locus_tag="IVP39_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP7586149.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVGAFFYCFIFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKANEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYSNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(18543..19253)
                     /locus_tag="IVP39_001068"
     CDS             complement(18543..19253)
                     /locus_tag="IVP39_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7586150.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATIATTTTTS
                     TESKPTAASTETADTSTSSESSKATETTQTSTSSSSVEASSSESSEQVEQILWNADKS
                     AQLEAFMATWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSE
                     SGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNET
                     ANNELKNGFNQIVYGQAN"
     gene            complement(19480..20022)
                     /locus_tag="IVP39_001069"
     CDS             complement(19480..20022)
                     /locus_tag="IVP39_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7586151.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIDNEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNMFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20027..20254)
                     /locus_tag="IVP39_001070"
     CDS             complement(20027..20254)
                     /locus_tag="IVP39_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7586152.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(20333..22213)
                     /locus_tag="IVP39_001071"
     CDS             complement(20333..22213)
                     /locus_tag="IVP39_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7586153.1"
                     /translation="MKFQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYMTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLVALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFAKIDTFVFDKTGTLTKGTTAVSTIKNYTENE
                     GDSLALVARLETLSDHPLGRAVIHYVQEKDIDFQQLAVKDNQTIKGQGITAEIDGHAV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSFVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(22403..23017)
                     /locus_tag="IVP39_001072"
     CDS             complement(22403..23017)
                     /locus_tag="IVP39_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7586154.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMGNELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKIKGIGTQLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            23159..23719
                     /locus_tag="IVP39_001073"
     CDS             23159..23719
                     /locus_tag="IVP39_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7586155.1"
                     /translation="MKKTDRRVKKTEKALAEALSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(24024..24338)
                     /locus_tag="IVP39_001074"
                     /pseudo
     CDS             complement(24024..24338)
                     /locus_tag="IVP39_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(24592..26232)
                     /locus_tag="IVP39_001075"
     CDS             complement(24592..26232)
                     /locus_tag="IVP39_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586156.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKRFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITTIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIVNSEGLVSIEGTQGETIIEV
                     SSIYPPTMKLAFLISLCSYLFLLISFLIRKLQHIKIMI"
BASE COUNT         8151 a         5246 c         4309 g         8945 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattc
     8881 cacattcact aagccatgtt ttttataaga gtcatttacg actttaccgt ttacttttac
     8941 ctgtgtacct aaataagcat atactggtac tgtcactttt tgctttttgt tgcttgccac
     9001 tgtgtaagta atgttattat ctgcagcttt atacttccct ttttcccacg attctcctac
     9061 ttttacttca tgtttttcta agtgaggcct ttcttttatc gcttctgttg gggcgtaatc
     9121 gtataatcct cccatatctg atgttgtata acgtgggagt gtttcttcat tgataacaat
     9181 atgaccgtgt tttgattgga taacttttgc gtaattttct tttgctgcta tagacgtgac
     9241 gagtagaaac accacagtag cgccaatgat taaccatgaa tattttttat tcatagtctt
     9301 tttcataaaa acaagcgtta tgatcattga agaaaaaaag atttggatac ctaagatacg
     9361 ccaaggaaac tggagcaagc ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt
     9421 agttacgcat acccatgtag aaactacgcc aacatagatg attttttctt ttttctccat
     9481 ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta
     9541 tgttgtaata tcgttattta acgcttgaat gatactatct gaaatagaaa gtgcttctag
     9601 ctgcaaatct gttttgaagg gactgtgtat ttcttgataa ttcatctgct gcaacatagg
     9661 ataccaaaaa tagctagtta atgcacaagt caaaaaaaca gcctttacta aattcacgac
     9721 cacaaatttt atatcgtttt ttatttgctt gcttaaaaac aaacgaacta taatcataca
     9781 agcaatcaat gcactacaca atagtgtagt taagacatga gtatacacaa gtagacttat
     9841 actgacagat aaagcgatga cttttttttc tctattgaat atcaatccat aagcataata
     9901 taaaacagct ggaataatcg taagagcgat tgcttctgca attgcggatc gataatagat
     9961 atcaactgtt cgataagttg caaaagtata taaacaacta aataaaaaag cagcatctat
    10021 ctttttaaaa aacttttctc cataataaaa actaatcaga atcgtacaaa tatttaataa
    10081 ccaaatatat agaacataac ttaatattaa gttatttgaa attccataaa aaacgactgc
    10141 aggaattatt gtaagaaaag gataaaaata gttcagtcca tctccataat tgttaaatgt
    10201 tgtatagttg attggccctt caaaaataga agataaccct ttgattcgtg aaagatgaaa
    10261 agataaatca cctgttgtat aaatttcttg ggcatttctc ataaaattta ctgaatataa
    10321 agtactaagt aagataagaa ctactaaaag aaatgctttt ttacttattt tcatagattt
    10381 gttgttctcc ttttttaatt ctattttcag tatactagaa ttgtcttttt ttaataagct
    10441 ttgcgttaaa tttttcatag cactagtatg atatactaga aaagaatcaa tttttattac
    10501 ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata tcatttttat
    10561 gttatttcta agttttgctt acgtcatccc tctattcatg tctaagggaa tctatcatag
    10621 tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata atgtatggag
    10681 ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata ttttttatcc
    10741 atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc ttgttttagg
    10801 ttataatatc tactattttt ttatcacttt tttaacaatg gtcgtttctt atttttctat
    10861 gaaacaaata aaaaacaatc gttatatttc gctgttattt tcgattattt atacattttc
    10921 cgcctatcgt gccattgaca tattccatcg tgcttcattg ggagaagctg tcgctttaac
    10981 atttttgcct ttgatactaa tggggtgtta cgaaatttat attagagatt accaaaaatg
    11041 gtactggttg tctattggca tgacgctagt agtgtatact catttacttt ctgtcgctat
    11101 ggtttctgta tttattggag gaacgttgtt tctttctttt tatttttggg accaaaaaat
    11161 cgctagactc ttaagtcttt taaaagctac cgccctcacc ttttttttaa gtgcgggatt
    11221 cttaattcct tttattcaac aatctagagc acaagaattg aaagtcccac tgggaaaaga
    11281 attaagtggt atggctccta gtgatatgct gacacatatt ttaaataata actataacaa
    11341 ctatactatt ggattattct tatttctcgg attgattggt gcttttattt ttataaaaaa
    11401 attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt tttgttcaac
    11461 caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac aatttgtctg
    11521 gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag tgatctatta
    11581 tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc ttgcactaat
    11641 attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat taacacttat
    11701 agcacctgaa aacgctgttg ctatagctga aaattacaat catactgatt atgcaaacaa
    11761 agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag gtaaccagga
    11821 aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag ataattcgaa
    11881 taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag tggcttctat
    11941 taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat taactattcc
    12001 tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa ttgcttcaag
    12061 atatttatct atcgttactt tgattttatt tttactttat cgatttactt tttctaaaca
    12121 aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag aaagactttt
    12181 tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat ttgcttcata
    12241 cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta tcaaagtatt
    12301 tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt tctcctgcat
    12361 gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg gcacctgtgc
    12421 cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat ccgtctccga
    12481 caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca atcgtacgca
    12541 ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta
    12601 atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa taaaagctgt
    12661 ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg tattttttgc
    12721 ccaccgcatt atatcttgaa tggaagcgct caggcacttc ttcgattctt ttgatgacaa
    12781 tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca atttctgtgt
    12841 ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc
    12901 caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt tttccttgaa
    12961 tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg tcatattcga
    13021 ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt ggtcttctct
    13081 ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga cagaagctat
    13141 acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc ggatgaagta
    13201 tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa gatactcaac
    13261 caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc
    13321 agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc
    13381 aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc attttttcga
    13441 tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa acctgatccg
    13501 atattatgga cccatgtcga gacattccac gaagattcat gggtattgat actgaacaaa
    13561 cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac tagggtagct
    13621 aatacacgag atgtacttac aaatgcagca tacaatgcag gcaatgacag gatgaaaaag
    13681 atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga aacctctcca
    13741 aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc taatacgtac
    13801 ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa
    13861 aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat tataaacgac
    13921 tttatcttga tttaacagaa acctctattt acacgaacat ctatataact cattaaaaac
    13981 tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat ccatctaaac
    14041 aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct cctattgcag
    14101 taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg ataacaccga
    14161 taataaatcc aataccaacg atttgcatcg cgacatcatt agagatacca aacaaactac
    14221 atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca caagcagcta
    14281 aagcagacaa aatacataaa aggactttca aaacaaatgg tgcttcaatc cctaaagaat
    14341 gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata ttgatcgtag
    14401 cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt tcagccaaca
    14461 tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt
    14521 ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa aacgtgagga
    14581 atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt aaaagctgca
    14641 gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc aatcctaaaa
    14701 tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct atagatatct
    14761 gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc aacaatacag
    14821 cccagaataa aacgccaagg taattcgcat caataagaga ttgaacagga ttttgtgtga
    14881 cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt tcagatactg
    14941 tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct acaagtgcag
    15001 aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc ttattcccaa
    15061 ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt gcgattgcct
    15121 tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga
    15181 taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac gacatatcca
    15241 aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt atcattttat
    15301 gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa ccatttcttg
    15361 ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag ttaggatgtt
    15421 attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg
    15481 tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat ctttgtcgct
    15541 tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc tacatatgca
    15601 acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt tattggtaca
    15661 aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta
    15721 cattatttaa gtcgttttgc ctttatattc ctaacagcca cactagcttg caatatcttt
    15781 ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct aacacctatc
    15841 agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag tattccttat
    15901 acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact attattttta
    15961 acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc attacagaat
    16021 ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt gaaagaatgg
    16081 aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt gattgtttta
    16141 ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa tacagataca
    16201 gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt ctcttcgttt
    16261 ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa ttttcttgct
    16321 gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta tcgcgtatct
    16381 acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa tatcgggcag
    16441 ttttttattt actatttatt agcagtgtcc ctcctagtta tctttttatt ttatatacaa
    16501 aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat tgaacaatta
    16561 acacaacgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta ttttttagtt
    16621 ggagcatttt tttactgctt tatcttcgtg cagattttcg ttttagaacc aaaagaaggt
    16681 tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc acccatgatc
    16741 ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc caatcgtttt
    16801 tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc gacagtaatc
    16861 aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct gaccggattg
    16921 tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt gatccttctc
    16981 ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta ttccttgaag
    17041 attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt ttcgggtgtc
    17101 tttttcatta atcataaaaa ttcgccttct ttcttgctgt ttaatttatt taaagtaaac
    17161 aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat tcaattccta
    17221 aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga aaaaattaat
    17281 ggaataatga aaaagtacaa cacaaaggca aatgaaatca accaaacaag aagtgactgg
    17341 ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc
    17401 cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat aacagaagga
    17461 acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat ggaatgggaa
    17521 ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc ttatacccag
    17581 ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga aaaaatagcc
    17641 atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa atttggtttt
    17701 gacaggttct attatttaga cagtccggat aaattaactt atacggataa aatagaacaa
    17761 agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt acaaagcaat
    17821 acagaaacgt cgcaattcat ccaattatcc actatgcaaa accatatgcc ttatgatgat
    17881 tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa tagaagaaaa
    17941 gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa aaaattcatc
    18001 caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga ccatttaccc
    18061 gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca gacagattat
    18121 ttcatttata gcaacaagta tagtcgtgaa caagcagcta cgttaaataa agaagttgtt
    18181 gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt aactccttac
    18241 tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga tcctacagaa
    18301 agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag tcaaattatc
    18361 caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg tctgatacaa
    18421 tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa aataaaagac
    18481 taacaaataa actgagcaag gggttgtgat aaaagacatg tcacaacccc ttgtttatct
    18541 cattaatttg cttgtccata aacgatttga ttaaagccat ttttcaattc attattcgct
    18601 gtctcgttaa agtaaagata attatcggga ttcccctgat tttgttgtgt caccaatact
    18661 tttggctgat tttgctgaaa cccgaataga tagacgtgct ttttcagata tggttctgtc
    18721 tctgcatcgg aatagacggc tgtgatttga taatctgctt gacctgtccc actttctgac
    18781 cattctgcag aaacaggcac tccaccaata gccatcttcc actccccgtt caaaatagct
    18841 tgagggactt taagaccata taagtccaca ctcatttgat tcgtatagct tttgtattgt
    18901 tgtcccattg tttggcccca tgtagccata aatgcctcta attgcgcgga tttgtctgca
    18961 ttccacagta tttgttcgac ttgttcgctg ctttctgaag atgaagcctc tacagaagaa
    19021 cttgaagtag atgtttgagt tgtttcagtc gctttggaag actctgacga agtagaggta
    19081 tctgctgttt ccgtacttgc tgcagttggc ttgctctctg tgctagttgt tgtagtagtt
    19141 gcaattgttg ctttcgctgt ttcttttgtt gattcttttt tcgattcatt cccgttggta
    19201 caaccagcta acaaaaaagc tagaccaatt gttactaaaa tccttttttt caattaaaaa
    19261 actcctttcg attactccca cttctttatt ctatcaaata agcaatgtga ttttattgat
    19321 tttttgtatt ttctcatcca tcttttaaac cttttaaaaa ctaagaagca cccgctgatg
    19381 ttgtctgtaa tacatcagcg ggtgcttctt ttattttaat cttcgtgatt ttccaattta
    19441 cctcacgctt tatatttgta tttgaagaaa catagacttt tagtcatcat cgtcttcctc
    19501 atccaaatct tgtcggacag tttttccttg gagtctttgt acttggtaga tgatttcttt
    19561 attgaaactt aaaagttcaa cgagtaattg ttgcaagcca tatttttctt ctttttcagc
    19621 taacttgatt gcccgtgtga taaacatatt ttggaaatca aaatcttttg caaagttttc
    19681 caacattgtt gaggctggta aatatttgtt ttgaccatgt tcgtaaattt ttccataacg
    19741 catgacttct tctaaagtag acggaacgat ttcattatca atcactaact catgagagat
    19801 tttattgaac catctcgctt cttgatcagc atattctgaa aaaagggtac ttaattcttg
    19861 cgaagatgta cccaaaacat acaattgtgc ttggatcaat ttcatttctt ctaatttaaa
    19921 atttgctaat acatgcccca ccatggcgcc agcagttggt ttatgatggt cgatatctgc
    19981 ttgttttgtt tcttgagcaa atttttcttc cactatattc actgatttac gcctctttta
    20041 cttttgttga ttggacttca taacctaatt tttccacaac tgcagctaat tgttcagggt
    20101 ggacttcatc tggatcaaag tttgctttga ttttgctcgc attaaacaaa actttcgtat
    20161 tgcttacccc aggctgttga gaaaccgcat gttcgatttt ttgcatacag cttggacaag
    20221 ctagtgtatc taattgcata ataatttttt tcatcgtagt tcttccttcc tttttcttga
    20281 tagtcttatt ctagattaca gcttccataa aaaaattgat tcttatcaag ttttacgatt
    20341 tttcttcttt gctttcgtgt ccaaaataca ataaacgcat cgcgttaaag ataacgatca
    20401 agatactagc ttcgtgaacg aacatcccac tagccatgta aataaacccg gcaaataagc
    20461 cgatgagtaa aaataagacg acaccaatgg caatcactac attttcacgg gtatttaaaa
    20521 cagttttctt agccaagcgg taggcatgaa caaacgatcc aaaactagat tgcatcaaga
    20581 caacgtcact cgtttcgata gcaacatccg ttcctgatcc catagcaatc ccgatatcag
    20641 ccgcagccaa agagggagaa tcattgattc catctccaat aaaagcaacg cgcaatcctt
    20701 gttcttggta ttttttcaca aattgaacct tttcgtctgg taataattca gcatggactt
    20761 catcaatgcc taacatatca gctacataat cagcagtcat ctgattatca cctgtcagca
    20821 tcactaactg ttttgctcct gcttgtttca acttagctaa ttgttcagcg acttctggtc
    20881 gaatcacatc tgatacacct atgatttgag tgattttttt gtcgattgct acaataatga
    20941 ctgagctgcc tgtttgttgt aattgatgta aatcttgtac ttgctttggt gtaagagaaa
    21001 gtttgtgagc ggcaatcaat ttggtgttgc ccgcacaaac tgcatgacca tcaatctcag
    21061 ctgtgatgcc ttgtcccttg attgtctgat tatctttgac tgctaattgt tgaaaatcaa
    21121 tgtctttctc ctgaacataa tggatcacgg cgcgccccag aggatgatcg gataacgttt
    21181 ctaatcgagc cactaaagct aagctgtccc cttcattctc tgtatagttc ttgatagtgg
    21241 atacagcagt agttcctttg gtcaatgtac cggttttatc aaagacaaaa gtatcgattt
    21301 tggcaaaagt atccattgct tcaccgcctt tgactaagat gccccgtttc gctccattac
    21361 cgattcctgc tacattcgaa acaggcgcac caatgactaa agcgccagga caaccaagaa
    21421 ccaaaacagt gattgctaaa cgaaaatcac gactgatcag accaacaagc aacgcaacaa
    21481 gtaaaacggt tggagtataa tactgagaaa atcgatcaat gaatttttcc gcatgggatt
    21541 tggtatcttg tgcttcttct actaattcga taattttggc aaaggtcgta tcatctccga
    21601 ccttagttgc ttcgatcgtc atatagccat ctgctaaaat cgttccagag aagacttcat
    21661 ctccactgat tttgacgact tgtcgtgatt caccagtcac tgcggcttca tccgcataac
    21721 cttttccgtc aatgactcgt ccgtccacag gaatagctcc accagttttt actaagacaa
    21781 tatcgccttc ttccacgtca tcgatttcca cttcttcagt cgttccatct tcttggacaa
    21841 cactagcagt cgatggggcc atctcggtca aagccttgat tgattcccgc gttttggtca
    21901 atgttttttg ttccagatag ctaccaaaca aaaacaagaa tgtaacgata gctgactcct
    21961 tgtattctcc gatcataaag gcaccaagca cagcaatagt aacgagtaaa tcaattgaga
    22021 taactttgac tttcactgcc tgatacgcat ggatcataat aggtatgacc gcaataatag
    22081 aagctatggc tagagcaatg ttatagaaca agacaaatcc agtaaatttt ccgataaaac
    22141 caaaaataat caacacagct gataaggcgg tgatatgatt tcggtgctct tggatccact
    22201 tttgaaattt catctttctc cctcttttcc ttttcgttct tacgagacta agtatagaga
    22261 cttatccatc ataaaaaatt gaccactatc aagtttagtc aaaaaggcgg ttttcttttg
    22321 gtagaatata ctacaaaaaa tagttagcaa gaagactcac taaaagtgat cttgccaact
    22381 attgtcgttt ctatcaaaat tttcattgaa tgattttcct gtacagcata catcggagat
    22441 ctatttcttt atctaagatt tttatcttgc taattttttc ctttagtctt tcaaatccac
    22501 gtcgttcata gaattgatac gtacagccgc tatctgtaaa aaggaatatt tcttttcctt
    22561 tctctctctt ttcaaattct gttagcaact gggtgccgat gccttttatt ttcaaatcag
    22621 gatttgcagc taaaaaaatg atctctccat ctggcgaatg cttctttaaa tatgctgcaa
    22681 gcatttgttc atttgctgtc tcgtaagctc ctttactttc tttagcaaat atattttgta
    22741 aaaaatcgaa tatcttaaca tatatttgct gtccaaaaga acgatgtttc tttttttctc
    22801 cttttatctc cgccagcaaa acacctgcca actcgtcctc tgcatacaaa gcaagaattt
    22861 gagttgctcg attcaactcc atatacaaaa aatatcttcc gtaaagattc agcagaagtt
    22921 catttcccat atatctctca aagttcattc cttcaacagc aaaatagata gcttttttgt
    22981 aatctttctt ttccaagggt ttaaaagtta gtttcatcat ttcgcctcct caacttcaat
    23041 gattgtgtta tacttacatt ataatagaca cagtgtctac tatctataat caatcatcta
    23101 taagattgtc gattaagcga cagctttgct ttgatatgtc ggataaggag ataggtaaat
    23161 gaaaaagaca gatagacgcg tcaaaaaaac agaaaaagct ttggctgaag cactatccac
    23221 attattagta aataaaaaga ttcaagcaat cactatacgc gaactaacag aaacggcaga
    23281 tgtacatcgt tcgacttttt atacacatta taaggatatc tatgatctat atgatcaatt
    23341 agaaagcaac ttttttcggg atctaaatga aattctctct tatgatccag cccatagcta
    23401 tgaggaacta tacactcgct tgattgacta tgtttacgca aatacttttt tatatcgctt
    23461 atttatcctc aaccatggtg atatttcgtt tcagaagaaa gttaaacaag ttattgaaga
    23521 aaactatctg aacatatggc tatatgaaga caagaaaacg attatcacag aagaaatgag
    23581 attcttcact acgtaccacg tccaaggttg tctatctgtt ctcaatcgat gggtagaaag
    23641 caattacaag tactccaaaa aagaattatt agaactctta cagaaactga atgatcacat
    23701 agagaaaatc atgccttgaa taaaaacata ctaagcttag ttaagaagaa ccgtaacctt
    23761 ctcgtataac aactgaatct tatttagcca aattgtttac tgaagatttt ttaagtaaaa
    23821 tttctataat tcctacttat tgaaaagtag tttgtagtgc tgcttctccc tctctaagtg
    23881 atatatcttg ttgtaccaat caaattcacc tcatcattaa taaattactt ttaggctaat
    23941 ttattacaac ttataggttg aagctgtaac caatttatag tcatattata tttttatcat
    24001 ccagtttatt gaattcttct cgtttacctc aattcatttt caagtggaat cagctcatca
    24061 tctaacattt tgcgccatgc ttgcttcatt tcttgtgggt atttaaatgg cttcgcttct
    24121 ggatgtttag ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagga
    24181 attggatctt cacctggcat atattgcaga ttagaaacat gtaatttttt ctcagaaaca
    24241 aaccactcat tggaaaagac atgaatcaaa tcatcactta cttgtgtaaa atagcgacgt
    24301 ttaatgacaa agatatcttc attgctatct agtgtacctt catttctttg tgcttagatt
    24361 caaagataat tttctcaata aagatttttg attccagtaa gattccttta ctccttttac
    24421 aacatagcgt tgtcctttgt tttgaaacat cttcttcccc tctttgccat taaaaatagc
    24481 ccaattctct ataaagaaga ttaagcttca ttattataat ataaagttta aatttggttt
    24541 attacttact ttttcttatt ctctagtaag ctaatattga aaaaaatgta atcagatcat
    24601 tatttttata tgttgtaact tcctgataag aaaactaatt aaaagaaaga ggtaactaca
    24661 taaactaatt aaaaaagcta acttcatagt tggaggataa atggaagaaa cttcaatgat
    24721 tgtttctcct tgtgttcctt caatcgacac tagtccttca ctattaacaa tctctctttt
    24781 ttgtccattt acttttgcga ctgtccccca ataatcaaat acaggaagcg ttattttttg
    24841 tcctttagat tcatgtactg tatagactat tttcgaatcc atcacttgat aaggcatagt
    24901 atgccattcc ttgccaattc ttacttcatg attctgcaaa tgttctttgt attgtaatgc
    24961 ttccgtgggt gcatagtcat aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt
    25021 ttcttgattc attaccaagt gatctctctg agtgacaact ttatgttcat agtttgcttt
    25081 tgttgcagca gatacagcca acagtaataa aacaattgtc gttatattaa ctacttttct
    25141 ttgcttagct gatgtgctct tcatgaaaac gaacgataaa atcaacgagc caaagagtac
    25201 ttgaaatcct aagattcgcc aaggaaattg aatcatttga atgggtgtat tttgtaataa
    25261 agaccatgga aaaaatttgg tggataagag ccaagagact accgcactaa ggtaaatcac
    25321 tttttctttg cgattaaatc ttttaaaaaa gaagagtgga cagatcaaag aaataatacc
    25381 aatgattcct accgtaaagg ttgtcaggtc attattcatc gcaccaatca aactatccga
    25441 gacatttaat gcttggtgtt gtaaggtcgt tttatctggc tcattaatcg attgaaccat
    25501 gatttgtcgg aacatcggat accaaaagta agctgttaaa agaatcgtca ttcctactgc
    25561 ctttgacaac tccacaatca accggatgct ttcttttttt cttgtttcac ttactacaaa
    25621 gccacctaag actagtaagc caacggctat tacactcata aaagtggaaa gaacatgtgt
    25681 ataaatgagt aagctcatac tgactgctaa tggaattgca catggctttt tctcataaat
    25741 cagtgcgtaa gcataataca taactatcgg tataaccgta agtgcaatcg cttcagcaat
    25801 agcagaacga tgataaatat caatcgtacg gtacccataa aatgtatata aacagctaaa
    25861 tataaaagcc gcatcgattc tttttaaaaa ttttagtcca taatgaaaca tcagcatgat
    25921 agtacaaaca ttcaatagcc aaacatataa gatataacta acgattaaat tattacttat
    25981 ccaataaaaa atgactgccg gaaaaaaggt caagtaggga taaaaataat tcactccgct
    26041 cccataatga ttaaacgtcg taaaattgat tggaccagaa aaaattgaag ataacccttt
    26101 gactctagca aaatggaagg caatatcccc cgatgtagaa atagtatgaa catttctaaa
    26161 ataattgatt gaataaagtc cagcaataat tgctaggaga atggacaata ttattttttt
    26221 ctttacttgc attacttaat tatatactcc tttcatggat tgctttatct agtataactt
    26281 aaattttaaa taaatcgtta tttattttta ctctcactta aaaaagcact aaaattataa
    26341 gagagggagg taatggtctg aacaccaaga attgttccaa catcgaattg cttaactaat
    26401 gagttcaaag cttaattatg tggctcttat atttaccgag cgtggattgc gatctcttta
    26461 gcgaaacaaa catctttcat aaaaaaaaca gccatatccg ctttcttgga tatgactatt
    26521 tactactact ttactatctt tcattcttct tgtctcactt atgataaggc gctgcgtatc
    26581 atgagtgaat gatattagga aaacttcata aaataaatct tttatacatt tccacttcta
    26641 gatggttatt c
//
LOCUS       DACCSK010000030        25895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCSK010000030 DACCSK010000000
VERSION     DACCSK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.030"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(178..585)
                     /locus_tag="IVP39_001076"
     CDS             complement(178..585)
                     /locus_tag="IVP39_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7586157.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(774..1601)
                     /locus_tag="IVP39_001077"
     CDS             complement(774..1601)
                     /locus_tag="IVP39_001077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP7586158.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1910..2128
                     /locus_tag="IVP39_001078"
     CDS             1910..2128
                     /locus_tag="IVP39_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7586159.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2149..2784
                     /locus_tag="IVP39_001079"
     CDS             2149..2784
                     /locus_tag="IVP39_001079"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7586160.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2977..3216)
                     /locus_tag="IVP39_001080"
     CDS             complement(2977..3216)
                     /locus_tag="IVP39_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586161.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3240..4763)
                     /locus_tag="IVP39_001081"
     CDS             complement(3240..4763)
                     /locus_tag="IVP39_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7586162.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4781..4903)
                     /locus_tag="IVP39_001082"
     CDS             complement(4781..4903)
                     /locus_tag="IVP39_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7586163.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4933..5916)
                     /locus_tag="IVP39_001083"
     CDS             complement(4933..5916)
                     /locus_tag="IVP39_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7586164.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="IVP39_001084"
     CDS             complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="IVP39_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7586165.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6110..6487)
                     /locus_tag="IVP39_001085"
     CDS             complement(6110..6487)
                     /locus_tag="IVP39_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586166.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="IVP39_001086"
     CDS             complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="IVP39_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7586167.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="IVP39_001087"
     CDS             complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="IVP39_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7586168.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7505..9277)
                     /locus_tag="IVP39_001088"
     CDS             complement(7505..9277)
                     /locus_tag="IVP39_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586169.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9231..10985)
                     /locus_tag="IVP39_001089"
     CDS             complement(9231..10985)
                     /locus_tag="IVP39_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586170.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11095..11664)
                     /locus_tag="IVP39_001090"
     CDS             complement(11095..11664)
                     /locus_tag="IVP39_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7586171.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11682..13106)
                     /locus_tag="IVP39_001091"
     CDS             complement(11682..13106)
                     /locus_tag="IVP39_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7586172.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13108..13776)
                     /locus_tag="IVP39_001092"
     CDS             complement(13108..13776)
                     /locus_tag="IVP39_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7586173.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13907..14491)
                     /locus_tag="IVP39_001093"
     CDS             complement(13907..14491)
                     /locus_tag="IVP39_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7586174.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14822..15055)
                     /locus_tag="IVP39_001094"
     CDS             complement(14822..15055)
                     /locus_tag="IVP39_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7586175.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(15070..16020)
                     /locus_tag="IVP39_001095"
     CDS             complement(15070..16020)
                     /locus_tag="IVP39_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7586176.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16017..16859)
                     /locus_tag="IVP39_001096"
     CDS             complement(16017..16859)
                     /locus_tag="IVP39_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7586177.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(16992..17717)
                     /locus_tag="IVP39_001097"
     CDS             complement(16992..17717)
                     /locus_tag="IVP39_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7586178.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18178..18492)
                     /locus_tag="IVP39_001098"
     CDS             complement(18178..18492)
                     /locus_tag="IVP39_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7586179.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(18508..20103)
                     /locus_tag="IVP39_001099"
     CDS             complement(18508..20103)
                     /locus_tag="IVP39_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP7586180.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20126..21016)
                     /gene="iolE"
                     /locus_tag="IVP39_001100"
     CDS             complement(20126..21016)
                     /gene="iolE"
                     /locus_tag="IVP39_001100"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP7586181.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21033..22058)
                     /gene="iolG"
                     /locus_tag="IVP39_001101"
     CDS             complement(21033..22058)
                     /gene="iolG"
                     /locus_tag="IVP39_001101"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP7586182.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22082..23089)
                     /locus_tag="IVP39_001102"
     CDS             complement(22082..23089)
                     /locus_tag="IVP39_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7586183.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(23202..24362)
                     /locus_tag="IVP39_001103"
     CDS             complement(23202..24362)
                     /locus_tag="IVP39_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP7586184.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(24438..25892)
                     /locus_tag="IVP39_001104"
     CDS             complement(24438..25892)
                     /locus_tag="IVP39_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP7586185.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         7945 a         5296 c         3974 g         8680 t
ORIGIN      
        1 aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa gatacttgtg
       61 cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa taaagcccct
      121 gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat ttccagatta
      181 gtaaatgtag taatctttgc tttctttttt ctcaatacta ataactaact tatggggtaa
      241 acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga tttgatcagg
      301 actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat tatattgtcc
      361 cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga agcgatccat
      421 ttccttgcca tcaatttgaa cgatggcata tttgatagca tcttcagttg tttcattctc
      481 cttttctata gagaatacaa ataagggaat aaaagaggga ataatcaaaa tgccaataat
      541 tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg atatctggct
      601 aaacatatta gaatttttcc atggaaatgg tgaaattttt cggaaattat ctgcattaag
      661 agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca tcttcatacc
      721 aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt ccttcaattc
      781 gggttttttt gtataatcat ttgacactca attttaggta aactagctac ccaataattt
      841 aatgattcaa tacttttatg gctacccgat gtttcaaaga tcgtcgaaaa atcactatgt
      901 tgggcaaacc aaagaaaccc ttttgctctt gtaatattgc caggaaaatt tttatccgta
      961 agatgggcaa atttttgtgc gtcaaggagt aaccgatttc gatagataaa acttgagata
     1021 ccatattctt ctgtttcagg aatatgattt tgatagccaa gctctaactc tttgaaccag
     1081 cccattgaat aggaagtctc atctatatta aaaagatttg tatttagaat tttcgataag
     1141 tctacttcac ctttaattgt cctaattatt ttggcattgg actgtaactt agcaattaga
     1201 ccgttaattt tgtaaatcgt cttggttgac actaagtcac tattatttaa aagtaatatg
     1261 ttacaacact cgaattgatc aattaacaag ccacgaatat cctgttcttc ttcaactaca
     1321 ttaccttcag cttttctttc ctgaatcttt tcacccgttt cgaaattatc ccaaaaacgt
     1381 tttacatcca ctactataat aatggcacca atctctaatc gttgggtcaa atcaatgtct
     1441 aaagggtttt tagctaatat aattgcttga ataattggaa ttggtttgct gattcctgaa
     1501 gcttcaataa caatttgatt caagtcagaa aattgcacta attgatctag ttcaacaatc
     1561 aaatcttcgc gtaatgtaca acaaatgcat ccattcggca tcgaaacaag tttttctcca
     1621 gttcttttaa aacctttctt gtcgactaat accccatcaa catttacttc ccctatgtca
     1681 tttacaatga cagcaatttc ctttccgtga tcaccgtaca gaatatgatt tagaagtatc
     1741 gtttttcctg aaccaagata gccagttaag attgtaatag gtattttttc atagggaacc
     1801 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     1861 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     1921 ttgtcatctt actatactca aacgagaaag aatctttctg cttcacgaag aaggattttc
     1981 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     2041 aagaaacaag aaaaaaacta atcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     2101 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     2161 ttatttgtcg attttcagtg gtcgccagag aaaatctcga atcgtttgaa gcatgaacat
     2221 atggactttt caattagttt tatcacgatc taccgtgcaa tttgcaatga tcttttcgat
     2281 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     2341 acaagacata caaaaaatca tgtcgagtct cgtgggaaga tttgccttac tcattcgatc
     2401 catggccatc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     2461 gtagctggca aaactagtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     2521 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     2581 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     2641 gaattctcca atcatttcgc agttacagaa gtattgaata atatgccctt ttattttcca
     2701 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     2761 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     2821 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     2881 ttgataaagt gttgcgcttg atttgacaat tcaagaaata ataaaaagca gatcaaatcc
     2941 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     3001 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     3061 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     3121 tgtactccca tttaatctca tttgctttgc aaattgataa cgccgataag catcaatatt
     3181 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa gattggattt
     3241 taatgggcca tcatttcttg agcaatctca tgctcagata atgaagctgg atagaaagta
     3301 ggccaattat gcatttcatt ataaagtttt tcttgacttt ctccaccaaa gaaaatatgg
     3361 aaatgctctg ccttacttgg tgcaattcca tgatcgctaa attgaatata tttaaaacga
     3421 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
     3481 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
     3541 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
     3601 taataatctt tgtactcggc agctgtcatg tctttattga gttttgcttt gtaatcgaaa
     3661 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
     3721 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
     3781 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttctct
     3841 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
     3901 tttgtaagac tttcaagagg attcaatact tccaatttta tcccagcttc attggccaaa
     3961 gtttttgcga ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
     4021 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
     4081 ccagcaatag gaacttgtat caatccataa tccaaagcta aatatccaaa ggctgcatgt
     4141 tgtgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
     4201 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
     4261 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
     4321 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
     4381 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
     4441 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
     4501 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
     4561 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
     4621 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
     4681 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
     4741 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
     4801 tattttgttt atgttctttg ataattttta atgccctttt tctagttttg atattcgggc
     4861 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccctaat
     4921 caatacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
     4981 ttcatccaac atttttccaa ttagaataat agtggtttgg tctttttgtc gagtggtgta
     5041 ttgaaaatta acctgttcgt ttaccccttg tatggcaact ataaaatctt gatcttgcaa
     5101 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccggtcaat
     5161 taattggaat aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
     5221 ctcatgttct tgatgatgtt gatgatagtg tttgccatta tagtgatttt ctgatgaatc
     5281 actttcaaaa actaagggat gattgaattt ttccaaatta taaaaatcct ttttttgaat
     5341 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
     5401 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
     5461 tgctatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
     5521 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
     5581 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
     5641 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
     5701 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
     5761 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
     5821 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
     5881 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
     5941 tatttcactt ctttgaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
     6001 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
     6061 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
     6121 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
     6181 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcaata cattgaaata
     6241 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
     6301 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
     6361 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
     6421 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
     6481 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
     6541 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
     6601 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
     6661 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
     6721 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
     6781 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
     6841 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
     6901 ttaattcctg acgtttatct gcgtactttt caatcatcgc catttgtttc tttgctttag
     6961 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacatctc
     7021 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
     7081 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
     7141 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaagctctg aaaaataaga
     7201 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
     7261 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
     7321 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
     7381 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
     7441 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
     7501 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
     7561 ttatgagatt tttgtatatt tctgaccttc gatacaactc actatgagta ccttgatcaa
     7621 ctatttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
     7681 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
     7741 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
     7801 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
     7861 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
     7921 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
     7981 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
     8041 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
     8101 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
     8161 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
     8221 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
     8281 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
     8341 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
     8401 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
     8461 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
     8521 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
     8581 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
     8641 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
     8701 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
     8761 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
     8821 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
     8881 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
     8941 gtaaatttga caaatcgtct gcaatttgca ttcttaaatg cgcactttct ttataagtac
     9001 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacga
     9061 acgatactgt aaaataaagc caaggcggct ttacattttc taaaactaat tcgtctatca
     9121 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaag ctagaaatag
     9181 atgctttaac caaatcttct gcgccctgat tagttaaagc gaatttgttt ttataaaaac
     9241 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
     9301 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
     9361 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
     9421 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
     9481 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
     9541 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
     9601 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
     9661 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
     9721 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
     9781 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
     9841 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
     9901 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
     9961 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    10021 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    10081 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    10141 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    10201 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    10261 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    10321 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattcct
    10381 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    10441 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    10501 agaaccaatc caattttaaa attaatgaaa aagcctaagg caactgataa aacaggtgtt
    10561 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    10621 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    10681 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    10741 atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca
    10801 gtttgaaccg cataactctt agctaaatat tcatctccta caataattag agacttaaga
    10861 aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa
    10921 gtggctaacc atccgtagta tttcatttta gggacataac taaataattt tttgtatact
    10981 ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct
    11041 ttccgtaata gcgtaaatta gagatgtaca aacagataga aggtaaacta cgttctattt
    11101 tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata aacttgctac
    11161 aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt
    11221 tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat
    11281 aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg
    11341 aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat
    11401 cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac
    11461 aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat
    11521 gattccagat aataatccaa aaatccctgg ggatatgctg tgaccaaacg ccccaccaat
    11581 actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat
    11641 aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta
    11701 aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt ttttattcca
    11761 ccatttttta aataaataat tctattgcat gttttataaa gaaattccgg atcatgagtg
    11821 gctataacta ctgcaccctt ttcttttaag tctttaataa ggtcagcaat cctttccata
    11881 ttaatataat ccaacccact tgtaggttca tcaaatagaa ataaattagt ttttgacaat
    11941 gaagctatag aaaccaataa tctttgtttt tctccaccgg ataaatcaaa cggtaattga
    12001 tctttcattt tataaagttc catttttttt aaaatttttt cttgctgatc tttactaact
    12061 atttcattcc ccagggctaa ctcgctcctc actgattctg tgaaaaattg gtaatccatt
    12121 tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat tttcgattat ctttcctttg
    12181 gtagggcgaa taatcttcat gagtattctt agtaaggttg ttttccccac accgtttttc
    12241 cctaccaaac caataacatc tccaactttg acatcaaaat ttatgtcttt tagaatattt
    12301 tcataagtaa tttcttcaac ttttaataca tctgcactta ccttttcgat atagggaact
    12361 tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt tattgttttc agtttttaac
    12421 aactgaatag ttgtagtttt tacagttcta tttttaacaa atagaacttg atcaattaat
    12481 tctttcaaat agtaaaaacg atgttcagct ataacaatag taaatccttt attttttaaa
    12541 tcacaaagta agtcagcaag tttcttagtc gctttgaagt ccaaatttga agacggttca
    12601 tccaatataa ttaatttaga gtttagcgat aaactacatc caatagaaat tttctgccgt
    12661 tcaccacttg atagcgaaaa aatgttttta tctaatatat ttgaaagatc taataattca
    12721 gctaattcat tgattctttt atccatatgc tcttttgtaa atccaaagtt ttccatcgaa
    12781 aatactaact cactcgttgt atttgttgta aagaattgtc ctctaggatc ttgacttacg
    12841 tagccaatat tttttgatat actttcttgt gttaaggatt gtagcgagtt attttcaaat
    12901 aaaatttccc caaccaattt tccgtcataa agtcttggaa taagtccatt cattaacttt
    12961 aatagtgtgg atttcccact cccagaagaa ccagtaatca atgtaagact accttttttt
    13021 attgaaaaat taaggtcaac aagcgcttgt tccttaacat ccttatatga aaaagaaaca
    13081 ttgttaaatt caaacaattc tttcacctca aatcaaaata cttaagaaaa aaatagtaac
    13141 ggaaaacagg atgatatagt ctaataagct catgtttgtt ttatagtaag aacttctttt
    13201 cacaggagat tctatcccct ttgttattgc attagcggtc atttcttcag caatcatagt
    13261 tgcacgtata agaacgggaa ctaacagaaa ttccaaatat tgcagaggct gaaaaaaaga
    13321 caagcgaatg tttctcattt ttaaagattc tttaatcatt cttatttcag aacgataagt
    13381 cggaaagaat ctgatcacta ctgtaatact taacattatt tttttcggta aatgtatttt
    13441 ttctaaagag gataataatt cattgctagt atagacacta accaacaacc atgagatcat
    13501 tacgaccggc aacattcgaa atacaatata taaggatgtc ccaaaaaata tagtaaaaaa
    13561 ctctatcttg atagcatcaa taattttcat tactaaaaac agaactgaat aaatcaagat
    13621 gaaataaata catgtcttta ttttacgaaa catcaatact aaaactgtag caataatgaa
    13681 acaccccact tgaaatatat ttccctttcc taaaaaaata atgaccggaa aaaaaatatt
    13741 taaagtaatc aaggttaacg gattgatttt tgacactctt tcacctccaa aaaataagat
    13801 cgcactgtta tcttaagctg ataaaaatgc accgttattc agataacagt gcgatcttat
    13861 caaaaagcat aactgagaac aattctcaat gcaagctttt ttttaattaa ctcagtaatt
    13921 caatccaccc aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt
    13981 ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt
    14041 ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat
    14101 ctagacaaca gactgtcttt tttgtcacaa atgatgtaat tttttcaaca atatcttgat
    14161 atttggttcc agctgattgg aataaaacca aatcgaatat acgtttattc tcaaaaatat
    14221 aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct atatctatat
    14281 tttctaagtt taagaggtat ttggtaaact cttcattata catattaata agcgcattaa
    14341 tatgcggctg aataatttct tcaaatagcg cttccttaga agtaaaatgt ttataaaaag
    14401 cacccgtagt tacgcctgcg gtcaagcaca gttgccttat cttagtttgc tgaaatccta
    14461 ctttttcaaa atggcttagt ccactttcta ttaatttttg gtgagttgta atataatctc
    14521 tggtttctat gggtacttcc tcctaataag atgaattaac tatattatct tacacaggac
    14581 ttgaagtttc aatcgttaac ctctattact gaactcattc cttagagcta aactaatgag
    14641 aaagagcaaa ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat
    14701 cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg
    14761 ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa aataaagcct
    14821 tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa
    14881 atcctatatc taaatagtcc tcatatctaa gtttagtcag catctcactt acagatgctg
    14941 gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata
    15001 attcctgaat tgctagtaaa taaattagta tagtagttgt tttaatttgt accataccta
    15061 ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc
    15121 atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata
    15181 gataggaata ttggtatctt ttgatactgt tttcattggt ctatcgtcca cactgctttc
    15241 tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc
    15301 tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc
    15361 tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat
    15421 tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc
    15481 tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat
    15541 atttttagcg taaataatac cgttttctaa atttaaccaa gcatggggat cttccttccc
    15601 tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc
    15661 tttgttttcc tctttgttcg cattttttac taattttgta aaccaagcat ttcctccagt
    15721 ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc
    15781 aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat
    15841 tttatctttt gctatatttt cagtaatatc tgctaaaatc gaattagtaa ctactacctt
    15901 taatttttcg ttcgaagcca ctgtatcttt gtctttacta tttgttgaac atgccccgaa
    15961 tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat
    16021 aactttctct ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca
    16081 aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca
    16141 atgaacaaac ctagcaatga tgagcacgct ccgactgtcg ccgaaataaa tatcatagtt
    16201 tttaaactgt ttgcatataa atatgctgta gcagctggtg taattaacat tgcaacaatt
    16261 aatatagttc ccacactttg catagctgtt actgctacta aagttaatag aatcattaat
    16321 agataatgat aaaaactgac attcatcccc attgcttttg ccattaatgg atcaaaagaa
    16381 gtgattaata attctttaaa aaatagaaca actgtaacca acactaaaac agaaacgcca
    16441 attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa gatatgaaat
    16501 aaatccgttg agctatttgc aactcctata aggataactc ctaatgctaa aaaagaactg
    16561 aaagtaatac caattgctgt atctcctttt attacactat tgctttttat aaaagtaata
    16621 atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata
    16681 taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt
    16741 cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta
    16801 attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata
    16861 ttattcccct tgtatattag agttagggtt ttaaccttcc ggttttgatt ggataaccct
    16921 atgaaccatc gttattttaa tttggtatta actcatttct tttgagataa tatatttatt
    16981 ggattgagag gctaagccgt tcccccctct ccaataaata tagtatctcc ataggctttt
    17041 tttaaattct cttcattaaa tacagattct acacttccgt gaacaattag atttcgattc
    17101 aaaagaataa tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc
    17161 actgtttttc cctgatgttt caattctttt aacgtatcca taattattcg ttcactaact
    17221 aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat
    17281 cttgctaaaa gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa
    17341 tattttccca tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta
    17401 attctttgaa aaactttcat tccggcatat gttcccatag atacacattc ttttactttt
    17461 attggaaaag taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt
    17521 ttattgatat cttcattatt aatggagacc tctccctgat gagggataat atttaacatt
    17581 gctttaagca atgtagattt tccagcacca tttggaccaa taatacctgt taaagcacct
    17641 tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc
    17701 tttttcactt ctaacataac tttctccttc taaatgataa tgattatcac gatcatatgg
    17761 tatacaaaaa ttatatttta gtcaacccta aattttataa tttagatttt tttctgttgt
    17821 tgaagattag gatgtcaaaa ttatttgaaa ctactcgtat agtaaatagt aagtaatttt
    17881 caaaacacga agaaaaacaa aaagaaaaaa ggaaacgccg aagcatttcc tttcagtgat
    17941 ttaatgagaa caacacgatt tgacttagag tctcaaaaag tcgaataggt acactcattt
    18001 aagtgtctcc attcgacttt ttggggatga gctggctatt gaggtccgct taccctattg
    18061 aataaataca agattgggta tttctaataa gtccgccctt catattgggg tgatcggggc
    18121 gaacatgcca tcttttcata taccacctcc tcgacactca ataatcaaag attctcgtta
    18181 atcttcttcg atgcttgcaa tgatgttttc aatattcata taacgaaaat cttcttgttt
    18241 gatataaacg atctgagcgg tacatttttc ctttttgatg atttctttta ctttttcctc
    18301 agaacgtgcg acctgcggcg ttaaaagtaa aacgtcatag tctttataat tcagtttttc
    18361 ttcaacgttt cggatcgttg cataatctat ctcatacgat agatttttgg ctctgatcgt
    18421 ttcaagcatt tttgatacga gcagcgacgc actgattccg gtctcgcaaa taatcaatag
    18481 atccttatgc ataataccct cctgttttta gcctgcaaag ccaattggtg aaaatgccac
    18541 ataagttaaa ataacaatga ctaccaatgc tatagcagtc accttggtgt atttccaagg
    18601 ctcgatatcg acctttgtat cgaatgagtt caacacaaat tctccatcag gtttcaattt
    18661 ggaaaccacc atcaaaatga taatgtccag aacgaataat gccgtcaaaa cataaaggaa
    18721 atggatattt tggaaaagca tttttgatag tgcatacaag acgatatgca cggcaatcgt
    18781 cgtttttgcc ccaaaagcag ttgctttttt tgaataaaag gcaaagagaa tgatcacgag
    18841 cagcggcatg ttgtatagtc cattgaattc ttgaacgaac tgatacaatc cagccggtgc
    18901 aaaagaaatg aatggcgcta cgataatgga gataatcccc acaacgaccg ttactgtttt
    18961 accagcacga gcgatttgct tatcatttgc tttcttattg ataagcggtt tataaaaatc
    19021 cagtgaaaac aaggtcgatg tcgcgttcaa agctcccgca aatgatgata agatcgcgcc
    19081 aaagataaca gcggcgaaaa taccaaagat cattttaggc aatacttcac tgactaaaaa
    19141 aggatacgcg ttatctggat ttgacattaa actatcgcca aaaatattgc gagcgacgat
    19201 ccctggaaag acaaggaata acgcacctaa gattttgaag acgccgacta ggaaagcgcc
    19261 cttttgtgcc tcagctaaat tcttgccgga caatgctttt tggacgatca tttgattggt
    19321 acaccagaaa tacagattgt taaacagcat ccctgtaaat agtgtcggcc atggaacaac
    19381 agccgagtcg atcgatccca cagagttaag cagccatggc gtgtttacca caacgtgttc
    19441 gatcccgcca acaaagctgc cgtcaccaag cttcattaag cctaaaattg ggaccagcag
    19501 accacaagcc agcagaccga ctccataaat ggagtcacta ttggcactta acgacaagcc
    19561 gccgatcagc agatagagga tgccgacaat accaattgcc atcgctacaa ttatgatcgc
    19621 gacgatcggc tggacgccca gcagctcatc aatatggaag atcttattga acaccagcga
    19681 tccagaataa agaaccacgg gtaagaaaga agtcatatag gtcacaataa ataagatcga
    19741 aataattcgt ttggtaaccg tatcataacg aatctcgatg aaatctgaga ttgtattgat
    19801 gccgtattta aaatattttg gcaagaatat cacagccaaa gctacgatcg caatcgtagc
    19861 agttacttcc caggccatta cttccatccc tgcatgataa ctttgaccat tttgtccgac
    19921 tatttgttcg gtggacaggt tcgtcatgat gatcgtgccg gcgattggca aagcagttaa
    19981 gctccgccct cccatgaaga acccttctga tccagaagta tcgacacttc tggatctaga
    20041 ataggcaaat aaccatacac agatgacaat cactaggaaa ctgattatag aaaaaagatt
    20101 catcgtctag ctccttttca atagattaaa gaccggtgac ctctcgaatg tagcttctgg
    20161 ctttgactgc atattcaaat ggattggctt tagccgggtc ctgttctgct tcaactacga
    20221 tccagccttc atagtcgctg gcatggattg ctgtccagat tggagtgaaa tcaatcatcc
    20281 catcaccagg aaccgtgaag acgcctttct tcacgccgtt taagaagctg tctttttcct
    20341 tttctacttc ttcagccgtt tcttgacgaa tatctttgaa atggatgtgt ttgatacgat
    20401 cttgatgacg gttgtaaacc gcgattggat tttcaccaga aaagaccaaa tgtcccgtat
    20461 cgaacaataa tttaactttg tctggatcag tcatttccat taaacgatcg atttcttccg
    20521 tcgtttgaac gccggtaccc atgtgatgat ggtaaacgat ctccatatcc ttttcgtggg
    20581 ccaaatcccc taatttttct aatccttccg ccagtttttt ccactcttca tcagtgaaaa
    20641 ctggtttatc atcaaagact ggcgctgtca taatcccttg gatactatga ccctgctcag
    20701 ctactacgat gactttagcg cccatcgcat gtaagaaatc ccgatgtgct ttgaaggctt
    20761 cggcagtttc ttcaaaaggt ttagttgtca gaaacgcact gaaccatgca ctggcaacct
    20821 ttaagccgcg aatattcaaa tattgttcca acacctctgg atcttttgga tatttgttgc
    20881 caatctctgt tccggtgaac ccagcaaggg ccatttcact gatacattgt tcaaaggtat
    20941 tttcactacc aagctcgggc atatcatcat tggtccacgc gattggtgcg atggctaatt
    21001 gaatttgttt ggtcatattc tcacactcct gcttattttt ttagttgtac taaactcttc
    21061 gtatcgaatg attcttgaca agccaacgcg atcttagttg attgcaatcc atcttctgca
    21121 ttaaccgctg gttgacgttt ttcttgaata catgagacaa attcttgggc ttcacgaatg
    21181 aaggcttctc tgaatcgttc tgggaagttt tgtgaacatg gacggatgat cccttgatcg
    21241 ttcatgatcg tcacaagatt tttctcaggc gcttgcgcta cccgcagcat tcctttggtt
    21301 cccatgatct ctgtttcgac atgatagcca tgggctgcgt ttctgccggc aactagtaaa
    21361 gctattgttt tatcgctcag gctcatcatt gcggctccag tttcaagctc accagcctga
    21421 tccaattctg gatacgccgc atttttccca atcgcccaca cttgatcgac ttcttggccg
    21481 gtaaaccagc gaaccaaatc aatatcatgg atcgccatat ctaagaacaa gccgccgctg
    21541 tcgttattca tggcaaattt gacaaagctt tccaagcctt cgctcggatc gatcccataa
    21601 caacgaatca gtgtgatttc gccgatttcg ccatcttcta ccattttctt cgcatactga
    21661 tacgactcat catagcggcg catgaagcct aacatgaaga cctgttcagg atgctcttcg
    21721 atgacttttt gggtcttttt aatatcttca acttcaaggc ctaatggttt ttcagtaaag
    21781 acatgcttgc cattttccat cgccaagcgg acttggtcac agtgaaagcc tgatggtgaa
    21841 acaataaaga ctgcatcgat cacggaactt ttgaccattt cttcatagct ggaaaaagta
    21901 tgcttgacgc ctaatttctt ctgtgcgaat gctaattctg tttcaactaa actgcacgca
    21961 gccgtaagct cacaacaact gacacctgtt gctaaatttt gtgcatgtac ttttcctaat
    22021 cttcctaaac cgacgatgcc tactctaatg ttgttcatga aatacctccg ttttctgaga
    22081 attactcatt gtatagtgct ggtcgttcat ccatttgaat cattactttt gtttttgttt
    22141 cacgagactc atgacaagcc atcatggtga tcgctgctac atacccatcc caagcggtag
    22201 gaccgacaac cgcatcattt ttgatcgaat cgatccacag cttgaattca taatcatagg
    22261 catccaaaaa gcgggtctgc caatctggtg aaacaggtgt gtaatttttt tcttccgatt
    22321 taattcgtgt ataggctggg tccgtcattc ctacttctgc cgtttcgaaa accacatcac
    22381 atttaatatc gtagccaaag cggcaattca caaagatttc caaatcaatc gttacaccgg
    22441 attttgtttg aaagatcagc agctgcggat catgcaaatc ggcatgggag ttgctggttt
    22501 ttttcggcaa gatcatttga acagattcat aatcttcatt tagcaaccag cgaagcacat
    22561 caatctcatg aatcgcggtg ttgctgatcg ccatcggtgt tgtgtagttt tcatctgcgc
    22621 taggatttct atgggcacaa tgcatcatta atgcttctcc gtattgcttc gattcgattg
    22681 cttctttcag ttcaatgtag ccgcggtcat agcggcgcat gaagccaacc tgaaccaatt
    22741 tcttcccata agcaacttcc gcatcaacga tccggcggca gcctgctgca tccatcgcta
    22801 atggcttttc gcagaaaaca tatttcccat gcttgattgc ttctaacaca tactgctcat
    22861 gggtcgggtc ccaagaagtc acaacaacga catccacatc ttcagaagcg atcaatgctt
    22921 ctccggtttc aaagaattta gcatcgacac gtttggcaac ctctttagca gcttttacat
    22981 tgatatctga gactgctgtc acataagccc cttgtgtttt ctgattgatc ctgtcgatat
    23041 gctcgatgcc gattgcacct gttccgataa cgcctacttt tactctcaat tacttatccc
    23101 cctgttctgt tgtttaagca cacatagtat gcgcttacaa aattattcct gtgtagcttt
    23161 tattcttaaa aggtagagcc ctctctttgc tccgcctttt actacccata agcttgtacg
    23221 tataattcct taatttgact atgactaggc acctttttat tattttgcgg acttccgctg
    23281 gtcaacgcat ccgcggccat tttgtctaag gctgccataa acgcttgctg atcaatacca
    23341 taatctcgaa tcgttgggat cgacagctgc tcacagagct cttggatttt tttaatcaga
    23401 taatccgcag cctcttccag cgataaatct tcggctgccg gataaataaa tctggcgatc
    23461 gtcgctaact cagtctgaat agcatccttt gagaattcaa aaaagaccgg caataacatc
    23521 gcatttgata tcccatgagg aatgtgaaaa agtgccccca ccggtcgact cattccatga
    23581 atcaatgtga ctgacgcatt cgaaaaggca atccccgcct ccgttgaagc taagctcatt
    23641 tgttctctgg cttcagtgtc gtcgctattt tcatatgcct gagaaagcgt gttcaaaatt
    23701 ttcttcatcg cttccagtga aaatagttgc gttacttcat tggctttgac tgaaacataa
    23761 gattccaaag cgtgacatag cgcatcgact cccgttgccg cggttacttt tggtggtaca
    23821 gttttcgtaa aatcagagtc aacgattgag atctgtggca tgaaaacgag atccttcatc
    23881 atcattttga ctgctgtttt tgtattggta ataactgcca catcggttac ttccgaacca
    23941 gttccagcag ttgttgacac tgcaatcagc ggaatgcccg cttgcagctg atcaaaattt
    24001 cttgcatgaa tcgcttcgag acttaattga ttagcagcca aaacggctac caccttggct
    24061 gtatccagac agctgccgcc accaaaagca atgatatgat cacattgatg cttctgaaaa
    24121 agcttcagcg catcataaac attttcttgc gtcggttcgg cttgaatgtc tgtatactcc
    24181 gctaattgat catctgtttt tcccaacagc tccttgatct tttgaagatg cccggtctgt
    24241 tccataaccg tatcactaat gattaacggc ttgttgccta gttgttctga ttcttccata
    24301 agaaattgga ttgttccgcc gccaattctt aaattggcag gcattttcag ttgaatattc
    24361 acagaaaaaa cctctctctg gcttatagcc tataacagaa tcctcgaagc tctttaacag
    24421 agcttcgaag caaatcttta aatctcactt tgcgcataac gtgctgtaac aactttccga
    24481 tgggtataaa actcaacact gtctttgcca ttggcatgca aatcaccaaa gaaagagtgc
    24541 ttccagcctg aaaaggcaaa ccaagccatc ggtgcaggca ccccaagatt gacccccagc
    24601 atcccagcat caacattttc tctgaagtag cgaatcgctg atgcgttgtt ggtatacaga
    24661 cacgaaccat tcgcaaattc agattcattc gtcagctcaa cggctgccat caaatctttt
    24721 acccgcacga ttgatgcgac cggggcaaaa atctcatcct gccagatctt catatccatc
    24781 gtgacattat caaaaatcgt tggtccaaca aaatagcctt tttctggaat gttttcgcga
    24841 ccatctagta ccagtttcgc tccttcatcg accccttttt ggatatagtc aaaggtccgc
    24901 tgctgtgcat cttttcgaat cactggtcct aaaaagaccc cttcgtccaa gccattgccg
    24961 atcttgatcg cttttgccgc agccttaaat ttttcaacaa actgatccgc gatttcctct
    25021 tccaaaacaa tgacggaggc tgccatgcag cgttcaccag cagaaccaaa agctccgcca
    25081 ataaggtcct tgactgcccg atcaatattg gcatctttca gcacaacaac atgatttttc
    25141 gctccagtca acgcttgtac ccgttttaga ttttttgttc cttcacggta aacgtattca
    25201 ccgacatttt ttgatcctac aaagctgatg ccttttacca gtggatcttg cagcaatgtg
    25261 ttgacaacat cgtgggcacc atagacaaca ttaaaaacac cgctaggaaa gcctgctttt
    25321 tcaaccagct caacaatttt ctccatcaac aatggtgttt tttctgaagg cttcagcacc
    25381 accgtatttc ctgccgccag agccatcggg aagatccaga agggcaccat cattggaaaa
    25441 ttaaacggtg tgatccctcc gattactccg attgggtaac gataattcgt gatctcaata
    25501 tcagtcgccg ctgttgataa agaatctccc atcattaaat tggtgattcc cgctgcgtgc
    25561 tccacatttt cgattcctcg ttgaacttct cccagtgact cggacaatga tttcccattt
    25621 tccagcgtga tcaaacgcgc caattcactt ttatgatctg ttaaaagctg atgcagcttg
    25681 aacagataac gtccgcgaac aggaacagga acttttttcc aagtttgaaa cgcagcatcg
    25741 gcagctttaa ctgcttcggc tgtctctgct ttgctagaca acggtacatt cgccagcgct
    25801 tcttctgtag ccggattcat tacttcttca aactctgttg agcgactagc gacccaacga
    25861 ccatcaatat aatttcttaa aaatcttgtc attta
//
LOCUS       DACCSK010000031        25348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCSK010000031 DACCSK010000000
VERSION     DACCSK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.031"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVP39_001105"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVP39_001105"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7586186.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IVP39_001106"
     CDS             complement(937..1518)
                     /locus_tag="IVP39_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7586187.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVP39_001107"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVP39_001107"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7586188.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKGFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IVP39_001108"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IVP39_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7586189.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IVP39_001109"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IVP39_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7586190.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IVP39_001110"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IVP39_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7586191.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IVP39_001111"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IVP39_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7586192.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7566..7874
                     /gene="rpsJ"
                     /locus_tag="IVP39_001112"
     CDS             7566..7874
                     /gene="rpsJ"
                     /locus_tag="IVP39_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7586193.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7905..8534
                     /gene="rplC"
                     /locus_tag="IVP39_001113"
     CDS             7905..8534
                     /gene="rplC"
                     /locus_tag="IVP39_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7586194.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8562..9185
                     /gene="rplD"
                     /locus_tag="IVP39_001114"
     CDS             8562..9185
                     /gene="rplD"
                     /locus_tag="IVP39_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7586195.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9185..9475
                     /gene="rplW"
                     /locus_tag="IVP39_001115"
     CDS             9185..9475
                     /gene="rplW"
                     /locus_tag="IVP39_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7586196.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9514..10347
                     /gene="rplB"
                     /locus_tag="IVP39_001116"
     CDS             9514..10347
                     /gene="rplB"
                     /locus_tag="IVP39_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7586197.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10390..10668
                     /gene="rpsS"
                     /locus_tag="IVP39_001117"
     CDS             10390..10668
                     /gene="rpsS"
                     /locus_tag="IVP39_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7586198.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10691..11038
                     /gene="rplV"
                     /locus_tag="IVP39_001118"
     CDS             10691..11038
                     /gene="rplV"
                     /locus_tag="IVP39_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7586199.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11052..11708
                     /gene="rpsC"
                     /locus_tag="IVP39_001119"
     CDS             11052..11708
                     /gene="rpsC"
                     /locus_tag="IVP39_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7586200.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11711..12145
                     /gene="rplP"
                     /locus_tag="IVP39_001120"
     CDS             11711..12145
                     /gene="rplP"
                     /locus_tag="IVP39_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7586201.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12135..12323
                     /gene="rpmC"
                     /locus_tag="IVP39_001121"
     CDS             12135..12323
                     /gene="rpmC"
                     /locus_tag="IVP39_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7586202.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12348..12614
                     /gene="rpsQ"
                     /locus_tag="IVP39_001122"
     CDS             12348..12614
                     /gene="rpsQ"
                     /locus_tag="IVP39_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7586203.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12672..13040
                     /gene="rplN"
                     /locus_tag="IVP39_001123"
     CDS             12672..13040
                     /gene="rplN"
                     /locus_tag="IVP39_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7586204.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13077..13385
                     /gene="rplX"
                     /locus_tag="IVP39_001124"
     CDS             13077..13385
                     /gene="rplX"
                     /locus_tag="IVP39_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7586205.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13413..13952
                     /gene="rplE"
                     /locus_tag="IVP39_001125"
     CDS             13413..13952
                     /gene="rplE"
                     /locus_tag="IVP39_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7586206.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13971..14156
                     /locus_tag="IVP39_001126"
     CDS             13971..14156
                     /locus_tag="IVP39_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7586207.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14192..14590
                     /gene="rpsH"
                     /locus_tag="IVP39_001127"
     CDS             14192..14590
                     /gene="rpsH"
                     /locus_tag="IVP39_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7586208.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14622..15158
                     /gene="rplF"
                     /locus_tag="IVP39_001128"
     CDS             14622..15158
                     /gene="rplF"
                     /locus_tag="IVP39_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7586209.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15319..15675
                     /gene="rplR"
                     /locus_tag="IVP39_001129"
     CDS             15319..15675
                     /gene="rplR"
                     /locus_tag="IVP39_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7586210.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAKAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15696..16196
                     /gene="rpsE"
                     /locus_tag="IVP39_001130"
     CDS             15696..16196
                     /gene="rpsE"
                     /locus_tag="IVP39_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7586211.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16211..16390
                     /gene="rpmD"
                     /locus_tag="IVP39_001131"
     CDS             16211..16390
                     /gene="rpmD"
                     /locus_tag="IVP39_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7586212.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16434..16874
                     /gene="rplO"
                     /locus_tag="IVP39_001132"
     CDS             16434..16874
                     /gene="rplO"
                     /locus_tag="IVP39_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7586213.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16874..18169
                     /gene="secY"
                     /locus_tag="IVP39_001133"
     CDS             16874..18169
                     /gene="secY"
                     /locus_tag="IVP39_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7586214.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18231..18878
                     /locus_tag="IVP39_001134"
     CDS             18231..18878
                     /locus_tag="IVP39_001134"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7586215.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19069..19287
                     /gene="infA"
                     /locus_tag="IVP39_001135"
     CDS             19069..19287
                     /gene="infA"
                     /locus_tag="IVP39_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7586216.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19320..19436
                     /gene="rpmJ"
                     /locus_tag="IVP39_001136"
     CDS             19320..19436
                     /gene="rpmJ"
                     /locus_tag="IVP39_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7586217.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19454..19819
                     /gene="rpsM"
                     /locus_tag="IVP39_001137"
     CDS             19454..19819
                     /gene="rpsM"
                     /locus_tag="IVP39_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7586218.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILANAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19847..20236
                     /gene="rpsK"
                     /locus_tag="IVP39_001138"
     CDS             19847..20236
                     /gene="rpsK"
                     /locus_tag="IVP39_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7586219.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20316..21254
                     /locus_tag="IVP39_001139"
     CDS             20316..21254
                     /locus_tag="IVP39_001139"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7586220.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21282..21662
                     /gene="rplQ"
                     /locus_tag="IVP39_001140"
     CDS             21282..21662
                     /gene="rplQ"
                     /locus_tag="IVP39_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7586221.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21934..22773
                     /locus_tag="IVP39_001141"
     CDS             21934..22773
                     /locus_tag="IVP39_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7586222.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22749..23618
                     /locus_tag="IVP39_001142"
     CDS             22749..23618
                     /locus_tag="IVP39_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7586223.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23608..24405
                     /locus_tag="IVP39_001143"
     CDS             23608..24405
                     /locus_tag="IVP39_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7586224.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24608..>25348
                     /gene="truA"
                     /locus_tag="IVP39_001144"
     CDS             24608..>25348
                     /gene="truA"
                     /locus_tag="IVP39_001144"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7586225.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKY"
BASE COUNT         8227 a         4682 c         5457 g         6982 t
ORIGIN      
        1 agttggcagg agaacaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgtactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catatttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aggacttctc cgttttcgtc
     1861 tgtacgcaag acaggttgga atcctttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatcccttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat tatggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact acactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg atcgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attacttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt ctcaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataatag aaggaacgcg aaataaaaga gtcagtattt
     7321 tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaatcctt gctttgtgag
     7381 tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca gacgtaatca
     7441 gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct ttgccacgaa
     7501 cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc gaaggaggga
     7561 acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac cgtattttag
     7621 atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc gtgtctggtc
     7681 caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact cataaataca
     7741 aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac attgtgaacc
     7801 caacaccaaa aactgttgat gctttgatga agctagactt accatctggt gtaaacatcg
     7861 aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc aaaggaatct
     7921 tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta attccagtaa
     7981 ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa acggatggct
     8041 acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac aaacctgcga
     8101 aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg
     8161 ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc caagcaggag
     8221 acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc
     8281 acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa
     8341 tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg
     8401 gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta
     8461 tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aatcactatc aaatcagctg
     8521 tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat gtagcattat
     8581 tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg
     8641 aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag
     8701 gaacacacgc ggttaaaaac cgtagcgctg ttcgtggcgg tggacgtaaa ccatggcgtc
     8761 aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg cgtggaggtg
     8821 gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa aaagttcgtc
     8881 gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg
     8941 aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt gctaacttat
     9001 ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct gctttatcag
     9061 cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg
     9121 tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg
     9181 cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat caatgcttgc
     9241 catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa
     9301 acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg
     9361 tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat
     9421 cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg aataagcact
     9481 aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta caaacctacc
     9541 tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac atcaacacct
     9601 gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa cggccgcatc
     9661 actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt
     9721 aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa ccgttcagct
     9781 aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc acctaaagga
     9841 ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt cggaaatgct
     9901 cttccattag aaaacattcc agttggtact gttatccaca acattgaaat gaaacctggt
     9961 aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg taaagaaggc
    10021 aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc tgcatgccgt
    10081 gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg taaagctgga
    10141 cgctctcgtt ggatgcgtaa acgcccgact gtacgtggta gcgtaatgaa ccctaatgat
    10201 cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc agtttctcct
    10261 tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc agacaaactt
    10321 attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca ttttgagagg
    10381 aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa
    10441 aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt
    10501 tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg acgtaaacat
    10561 gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt tgcacctaca
    10621 agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt tgaggggagg
    10681 ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa cagttcgcac
    10741 ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc
    10801 aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa aagtattgat
    10861 gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact tggtagtaag
    10921 cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc
    10981 agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag aaaaataagg
    11041 agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc atcatccgcg
    11101 actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac gaagatttaa
    11161 gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg
    11221 aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt atggttattg
    11281 gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa
    11341 gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg
    11401 aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca
    11461 tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa
    11521 acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct cttcatactt
    11581 tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga aaactaggag
    11641 ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact gagaaaggag
    11701 ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat
    11761 gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt tgcaagctgt
    11821 tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa tgactcgtta
    11881 catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt atactgccaa
    11941 agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg ttgcaccagt
    12001 aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc
    12061 gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac gtgaggaaat
    12121 gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg
    12181 atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta gcaacaggtc
    12241 aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc atcaaaactg
    12301 tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg actgaaggaa
    12361 gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat aaaacaatta
    12421 ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg aactattcta
    12481 agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta
    12541 tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta gtcgaagaag
    12601 cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa tctgaaagga
    12661 ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac
    12721 gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct aacatcggtg
    12781 atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg
    12841 tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac ggttcatata
    12901 ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct cgtggaacac
    12961 gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac
    13021 tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa acagttatgt
    13081 ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac aaagaaggcg
    13141 ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta aatgtcgtta
    13201 aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag atggaagcgc
    13261 caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc cgagttggct
    13321 acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa gttttagata
    13381 aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa aaatatctta
    13441 aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg caaacaccta
    13501 aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact
    13561 tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg attacaaaag
    13621 ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt gcaaaagtta
    13681 cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt tcactaccac
    13741 gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga aactatactt
    13801 taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta gataaagtac
    13861 gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct cgtgagttgt
    13921 tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac gtggctaaaa
    13981 aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct tatactcgtt
    14041 gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc cgtatttgct
    14101 tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag
    14161 taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa ttgcagattt
    14221 tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag tacctgcatc
    14281 aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga
    14341 atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg
    14401 cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa
    14461 agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt cgacttctga
    14521 aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta
    14581 cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt atcggaaata
    14641 aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac gttacagtta
    14701 aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg aatatcgaag
    14761 gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca attcatggaa
    14821 caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc caaaaagcgt
    14881 tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt gtattgaacg
    14941 ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt gaagtaccag
    15001 ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa
    15061 acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt tatgttggcg
    15121 aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa attgcataga
    15181 agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat acatttttac
    15241 ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta gtacaataaa
    15301 aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg tcaaaagaga
    15361 catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt gaacgttttt
    15421 cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt aacgctagca
    15481 agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc agcaaaagct
    15541 gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt agtctttgac
    15601 cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat
    15661 ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat ccaaaacatt
    15721 tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt aaaggtggac
    15781 gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac gtaggatttg
    15841 gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa gatgcgaaga
    15901 aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta atcggtgctt
    15961 ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt gctgctggtg
    16021 gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct aaatctttag
    16081 gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa caattgaaac
    16141 gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc ggataaggag
    16201 gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg gacgtcctca
    16261 aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt cagtggttaa
    16321 gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag tggacgttga
    16381 agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa cctatgaaac
    16441 ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta
    16501 cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa gctcgttcag
    16561 gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt cgtttaccaa
    16621 aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt gatgtattga
    16681 accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct ggaatcgtga
    16741 aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag aaattaactg
    16801 tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct ggtggatcaa
    16861 tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag acattagatc
    16921 gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt
    16981 accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt tcaacatgtt
    17041 gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc
    17101 atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt
    17161 tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga ctcgatatct
    17221 gacattagta ttggcattta tccaatctgt cggtatcact gctggattca atcaatacgc
    17281 tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg gtttgattct
    17341 aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa aaggtatcgg
    17401 aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg aaagtatcag
    17461 agaaatttat gaagattact ttataaatat tgatccttca gacatatgga aatctgctat
    17521 ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa catttttcca
    17581 acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg cgcctacaag
    17641 cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct ttgctagttc
    17701 gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg gttctgaaaa
    17761 ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg ctacgattta
    17821 tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa accctgagaa
    17881 attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc ctggtaaggg
    17941 gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg cagtcttcct
    18001 tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc cacaatcaat
    18061 cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca
    18121 gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag aaagcgtgtt
    18181 gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca atgaacctca
    18241 ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt
    18301 atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa aacgaaacag
    18361 ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct gatgaagtaa
    18421 caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc ttcttattag
    18481 atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta aaagatttga
    18541 ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg gtcgaacgtc
    18601 tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt ttcaacccta
    18661 caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa agagaagatg
    18721 ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt gaaccaatct
    18781 tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt gaaatcgatg
    18841 ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg tagccgactt
    18901 gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt cagtccgact
    18961 tctagagaat cctctagatg atccattttg agaattttga ggtgggaact gcagtttccg
    19021 ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa
    19081 gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt
    19141 gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg tatgcattac
    19201 atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga tcttaaccgt
    19261 ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga ggtattatca
    19321 tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag
    19381 gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa ggataattgg
    19441 aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata aacgtgtagt
    19501 agtttcactt acttatatct atggtattgg taacactact gctaaaaaga tcttagctaa
    19561 tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat
    19621 ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag tgaacttgaa
    19681 catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc
    19741 tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat
    19801 cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa
    19861 agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac atatccattc
    19921 tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat tagcgtggtc
    19981 atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg ccgctcaaat
    20041 ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg ttgaagtaac
    20101 agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag ctacaggttt
    20161 agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc gccctccaaa
    20221 acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg tcattgaaca
    20281 agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa
    20341 tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct cttgaaagag
    20401 ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct ttgccaggtg
    20461 ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc atcaaaggtg
    20521 ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg aaaatgtatg
    20581 gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta actgctggcg
    20641 atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct
    20701 ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag
    20761 cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat tcaatctaca
    20821 cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac cgtgaagatt
    20881 ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg gaagcaatga
    20941 gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta actgatgaag
    21001 caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa
    21061 tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa
    21121 tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa gtccgtaact
    21181 taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt ttaggtttac
    21241 gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat cgtaaactag
    21301 gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat ttattaatca
    21361 acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa
    21421 tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac
    21481 gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa tcagctttac
    21541 aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga tacacacgca
    21601 ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct
    21661 aaaatcacac taataacatc actttataga tgattctttt agagtgttat gatgttggaa
    21721 gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag
    21781 cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga tatgtttgaa
    21841 ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt
    21901 tagtaggaac tttaggatga agaaggagta atcatggagc caattattga attagaaaag
    21961 ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt ctcctttaca
    22021 attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa atcgacactt
    22081 gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa
    22141 atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatggt ttttcaaaat
    22201 ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat
    22261 caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga gaaagttaga
    22321 atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt
    22381 gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga agcaaccagt
    22441 atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat caaagaagaa
    22501 agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa tgccaatcgg
    22561 atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga aattttttct
    22621 gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa actgaagagc
    22681 gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga aaggatggtg
    22741 gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg aatacaccat
    22801 ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt tatactgcat
    22861 tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat gcgcttgtta
    22921 agccaacaag cggtacagtc catattggag aacgagatat tcagccggat acggataata
    22981 agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc
    23041 tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg
    23101 aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg gatgaaagct
    23161 atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta gcaatcgctg
    23221 gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca ggacttgatc
    23281 cccagggtag aaaagaaatg atggagatgt tttggcgtct tcataaggaa cagcagatca
    23341 ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat tatgtctatg
    23401 tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt caaaacattg
    23461 aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca gaagaattaa
    23521 tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta gcagacgcta
    23581 tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg tcgttatatg
    23641 cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc cagtttttat
    23701 tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt ggcatttttc
    23761 acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg aggcattcgc
    23821 ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac aagtggcgga
    23881 gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat gaacggcctc
    23941 tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac gcttactaca
    24001 ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa agccgtacgg
    24061 ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt gcctacattg
    24121 atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga ttttggtgaa
    24181 ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt atttgtcagc
    24241 agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta caaaggtggg
    24301 gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat ggttatcatt
    24361 gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt cagtgggccg
    24421 ctatctattc ttttcttgct gatctgtttg caagaaagga tgcgagcagt gtatgacaag
    24481 aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc tgaaaagaaa
    24541 gaacgaaaaa attttataaa aaaatcatta ttccagataa ccttttagga acaaaggaga
    24601 gaaaaacatg gtacgttaca aagcaatcat tgcatacgac ggaacaaatt ttaatggatt
    24661 ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat tacaaaaaat
    24721 ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg gagtacatgc
    24781 gattgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac gaatgcgttt
    24841 cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa ttgtttcaga
    24901 agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag tggatatagg
    24961 gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc ctcttgattt
    25021 ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca cctctttttg
    25081 tgcatcagga agctcgatag aggataaagt acgtacgatc tacgaggcta agatggaggt
    25141 aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct tgtataagat
    25201 gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc ctgaagatag
    25261 tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag cacatcctga
    25321 aggactttat ttgtatgaag tgaaatat
//
LOCUS       DACCSK010000032        24979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCSK010000032 DACCSK010000000
VERSION     DACCSK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.032"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(525..1232)
                     /locus_tag="IVP39_001145"
     CDS             complement(525..1232)
                     /locus_tag="IVP39_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586226.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1297..2133)
                     /locus_tag="IVP39_001146"
     CDS             complement(1297..2133)
                     /locus_tag="IVP39_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7586227.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2151..3560)
                     /locus_tag="IVP39_001147"
     CDS             complement(2151..3560)
                     /locus_tag="IVP39_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7586228.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3574..5397)
                     /locus_tag="IVP39_001148"
     CDS             complement(3574..5397)
                     /locus_tag="IVP39_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7586229.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5677..5904)
                     /locus_tag="IVP39_001149"
     CDS             complement(5677..5904)
                     /locus_tag="IVP39_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586230.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6240..6566)
                     /locus_tag="IVP39_001150"
     CDS             complement(6240..6566)
                     /locus_tag="IVP39_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586231.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6612..6974)
                     /locus_tag="IVP39_001151"
     CDS             complement(6612..6974)
                     /locus_tag="IVP39_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7586232.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7011..7226)
                     /locus_tag="IVP39_001152"
     CDS             complement(7011..7226)
                     /locus_tag="IVP39_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7586233.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7353..7688
                     /locus_tag="IVP39_001153"
     CDS             7353..7688
                     /locus_tag="IVP39_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7586234.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7764..9761)
                     /locus_tag="IVP39_001154"
     CDS             complement(7764..9761)
                     /locus_tag="IVP39_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586235.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9859..10134)
                     /locus_tag="IVP39_001155"
     CDS             complement(9859..10134)
                     /locus_tag="IVP39_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7586236.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10366..11100
                     /locus_tag="IVP39_001156"
     CDS             10366..11100
                     /locus_tag="IVP39_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7586237.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11139..12281
                     /locus_tag="IVP39_001157"
     CDS             11139..12281
                     /locus_tag="IVP39_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7586238.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12376..13767)
                     /gene="sufB"
                     /locus_tag="IVP39_001158"
     CDS             complement(12376..13767)
                     /gene="sufB"
                     /locus_tag="IVP39_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7586239.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13772..14242)
                     /locus_tag="IVP39_001159"
     CDS             complement(13772..14242)
                     /locus_tag="IVP39_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7586240.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14229..15464)
                     /locus_tag="IVP39_001160"
     CDS             complement(14229..15464)
                     /locus_tag="IVP39_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7586241.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15461..16747)
                     /gene="sufD"
                     /locus_tag="IVP39_001161"
     CDS             complement(15461..16747)
                     /gene="sufD"
                     /locus_tag="IVP39_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7586242.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16760..17533)
                     /gene="sufC"
                     /locus_tag="IVP39_001162"
     CDS             complement(16760..17533)
                     /gene="sufC"
                     /locus_tag="IVP39_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7586243.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17730..18578)
                     /locus_tag="IVP39_001163"
     CDS             complement(17730..18578)
                     /locus_tag="IVP39_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7586244.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18602..19288)
                     /locus_tag="IVP39_001164"
     CDS             complement(18602..19288)
                     /locus_tag="IVP39_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586245.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19281..20315)
                     /locus_tag="IVP39_001165"
     CDS             complement(19281..20315)
                     /locus_tag="IVP39_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7586246.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20710..21048)
                     /locus_tag="IVP39_001166"
     CDS             complement(20710..21048)
                     /locus_tag="IVP39_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7586247.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21050..21406)
                     /locus_tag="IVP39_001167"
     CDS             complement(21050..21406)
                     /locus_tag="IVP39_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7586248.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21412..22599)
                     /locus_tag="IVP39_001168"
     CDS             complement(21412..22599)
                     /locus_tag="IVP39_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7586249.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22790..23635)
                     /locus_tag="IVP39_001169"
     CDS             complement(22790..23635)
                     /locus_tag="IVP39_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7586250.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVGSHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23869..24834
                     /locus_tag="IVP39_001170"
     CDS             23869..24834
                     /locus_tag="IVP39_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7586251.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7310 a         5245 c         4238 g         8186 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
       61 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
      121 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
      181 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
      241 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
      301 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
      361 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
      421 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
      481 taatcagtta cctgtccgtt cttaaatacc acagaataat ctttttaccc attttcaagt
      541 aaccgaagtg gaatgtcttt accaacttgc tctgtcactg cattactgat acctgttaga
      601 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
      661 gtgattcctt tatcttcatt tacaagcttc caagtaggtg gtatagctgt gtatgtttcg
      721 atgaatgaat caattctttc atctatcgtc ttcccagtat ctacttctgt ctcattactc
      781 ttagatgaat cgattctttc atccatcgtc tgctccgtaa ctgcttctgt ctcattattc
      841 tcaccggaaa ctctcttcac acccaaactt atatattgtc catctttagt gtgcatagtt
      901 aaaattccca cgcttttact aataaattca ctataaccag ttctttcaat agagacttca
      961 atgtatttca cactatcata ctcaaaagaa aaatagacat gtaaaagcat tttgttgttt
     1021 acatctacat aataatcctt tgactctact tgagaccttc ctatacggaa tacagcttta
     1081 ctgtctgata tctctatacc agcatcctta ttattaatct gccaatctcc aagtagctct
     1141 tctggcaagc tagtagtttc ttgtgaacac tttaccactt cactggtaga ggtcgaaaca
     1201 ttcacctccg cactgttaga agaattttcc aaggaatcag gagccttagc acaaccaaca
     1261 agtctttata tattgtgcag ccgatatttt cttcattcag tacacccttg aaatatgcag
     1321 tgttagataa agcagttcgt cctgatttgc tgaggcaccc atctcttctt gaacgtactc
     1381 cttgattttc aaggccgccc gatactcttt cggaaaacgc tgcttcactg aagccagcaa
     1441 aaaatcatcc ttctccggca actcttcctt cttaatcatt cgggcaataa aaaactgtaa
     1501 atgcgtcaca aatcttaagt aaagcatcga atctttatca accttttttt cgggaaacag
     1561 atatcctagc atagccaaga tatcctttag tgctcttacc gagagcatgg tttcttccat
     1621 atttgcttgt tcactttggg catttacaat gtgaaacgca atgtttccag cctcctcttc
     1681 tggcaactga acattcattt ttttgttgat caattggata gcatagcagc cgatctcaaa
     1741 ttcttttggg taatactttt gaatttcaat cagcaggcga ttttgaaaaa caatgccttt
     1801 ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag gtaaaaaata gacttttatt
     1861 cagcttaaag tcaaacttgg cgttggcata atcgataatt cgcttgacgc aaataattag
     1921 atcatccgga gcagcctcaa acagtttaat gtaatccttt tttagagcat cagaaagtga
     1981 atctaattta aatacttttt ggattttgtc agccggaacc aaatcgcctt tctttagttg
     2041 aaatccaatc cctttcccca tcaaaattac ttctttgcct tgcttatcta agcaaagaat
     2101 taaactgttg tttaacgttt taattactct cattagattc accgttctct ctaagctaag
     2161 ccatttcttg cgattacttt ttgataccaa aagaagcttt ctttttttat tcgttttaat
     2221 tctttaagat tctcatcggt tcggtcaaca tatataaaac cataacgctt attgaagcct
     2281 tgatgggtac taacaacatc aatcgctgac caaggtaaat aaccaaaaac atctacacca
     2341 tctgttaaag ctgcttgaat agccagaata tgtttggcta gataatcaat tcgatcctgg
     2401 tcattaattc gtctatcttc ctcaactatg tcgggtgccc catagccatt ttcagtaatt
     2461 aaaataggta aatcatagcg atcgtacaat ttcctcaatg ttaatcgtag tccagtaggg
     2521 tcaactaccc agccatatgg cgttttatct acataaggat tttcagctgc ccggtagaca
     2581 ccttcttctc ccaacatgat ctgttgatcc cccgctctag cggaaacatc tgatccgtca
     2641 tttttgctgg cagcgatggt ggcagttgaa taataattaa tcgcaataaa atcgggttta
     2701 ccggaggata aaattgcctg atcctctgcc tcaattttag gatataatcc acgatcattc
     2761 aaatagtttt caaacaaata attgtatttt ccccgaactg ctacatctaa gaaagaccag
     2821 cctctcaatg tttcccaatt atgggctgca attgcatctt ctggactgga ggtagcttga
     2881 tacatagccg ttaaattgat tgctggaccg atttttcctt taagattcaa ctgatgatac
     2941 aagtgaatta ctctagcttg agctaataaa acatggtgat taatttgaaa gagttctttt
     3001 tttgaaggca gctctccact tggtggcaaa ccaatcgctc caggatgaag gatcatcgtg
     3061 ttttgctcat taattgtcag ccaatagttc accttgtcac catactcagt aagcaatacc
     3121 tgtacatagt ccacaaaata atcgatagtt gctcgattta accaaccgcc ctgctcctct
     3181 agaaaataag gtaaatcgaa atggtacatg gtgacaatcg gttcgattcc ctgattcagg
     3241 cattcatcaa ttacttgatg ataaaaagaa attccctttt gattggtttt attattttca
     3301 atatccataa ttcgcgtcca agcgatggaa aagcgaaaag cttttaagcc taattcttta
     3361 aacagctgta tatcttcttt atagtgatgg taaaaatccg aggcaacttc aaaattagct
     3421 accccttctg tatgctgata cttgtcaata atagacggtt gtttcccatc ctcctgatag
     3481 gcaccctctg cctggtaggc actaatggag gcaccccata aaaacttctc tggaaatgct
     3541 gaaagctgtt tgtattgcac aaaaatcctc tccttaacta atttgcatca atggttcgtg
     3601 ttcctttaag cttccgcttg cttcactaat tcctgcgtaa ttttcactgt tggtcactac
     3661 cacaatcact gtatcatcgt agccagcgtt tctaatggca gcgaaatcgg cgttgattaa
     3721 taattgtcct gctgacacct gatctcctgc ttttacaaat acttcaaagg gttccccttt
     3781 taaattcacg gtgtcgatcc ccacatggat caataattca ataccttctt tagaacgtag
     3841 accaatcgca tgtttagatt ctaaaatagt aatgacttct ccctcaacag gtgaataaat
     3901 gctgttttct tctggatgaa tggctacgcc ctttcccaat agtcccttag aaaagacagc
     3961 atcgttcact tcagaaatag gacacacggt tccttttacc ggattttcaa caatgactgc
     4021 ttcggctatt gccgtttgct ctgattcatc agaagtatcg tcaaatccaa ttagataagc
     4081 aatgataaag gttgcaacaa aggcgattac acacgtaata attgcgtgaa caatattcat
     4141 tggattttct ccaataaagg ctggtaaggc tgctaagcca ggggaaacaa aggcatatct
     4201 gactaaaccg ctcaagccag catacaaacc agcaattgca ccaccaatca tggaagcaac
     4261 taatggtttt ttcagcttca aggttactcc atagagtgaa ggctcagtaa ttcccattaa
     4321 agcagtgatc ccagtggaca cagctagctg cttcaactct ttgtttttag ttttcgcggc
     4381 tacagcaaag gtagcacctg attgagcaat attagaagcc agcattcctg gcccattgat
     4441 catttcagaa cccgtactgc tcaattgcat agtagcgatt ggcgtcattg cccaagctgt
     4501 acccgtaacc accaaaaatg gctgcaaacc acccatcaaa aatggaatta accaagctgc
     4561 tttgccgtta atccattcag cccccattgc tataagatcg ttcagcaacg ttccaaacgg
     4621 tccaatgaca accaacgcta aaggccccat aattaaaaat accagcatcg gcttagtaaa
     4681 gaatttaata aacgaaggcg aaattcgatc cgcaaagggc tctacatagg acatgatcca
     4741 aacagtaaag ataatcggta aaactgatcc cgcataatct gcagctaata ccggaattcc
     4801 tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa atatttggat gaagcaaaat
     4861 gccagcaaca gtaatcgcca aaattgggtt gacatcaaac tttatggctg ccccataggc
     4921 taatagtact ggcataaaat aaaaggctgc atcagcaata gtattcaaaa tgatgtaagt
     4981 ttgactactg tctgatagta caccagtcaa agtcaagacg gccaaaatgg cttttaccat
     5041 cccggcacct gttatagctg gaatcatcgg tgtaaaggtg gtagaaatga cactaatcaa
     5101 tcttgaaata atactttctt gattgtctga agaggggccc tttttaattg aagttcccac
     5161 ttccttttga atttccttga aggctgtttg gacttcattg ccaatcacca cttggtattg
     5221 tccaccgcta tccaccacac taattacacc aagtgttttt tctaaggctt tcgtatcggc
     5281 tttcctttta tctttgagtt caaaacgcag tctggtggca caatgggtta agctgaccac
     5341 attttctttt cccccaacct gttgcaatac atttcttcct acttctctgt aatccattat
     5401 tctctctcct taaagcagta aaaaagacca agtcaaacca cgccccaatc aacattgaag
     5461 tgttagtttg tcttggtctt gcctgcataa tcagtaacat gccaataacg ttattttgct
     5521 gtaatccaat aatagcacat tatgatagcg caatcaacag ggaatttaat tttttatctt
     5581 ttcgtttgac cgtatagggt aatataacag ttagatcaca tcaactaggg tatatctgcg
     5641 tatacttact gctaaaataa gtatatctaa tattaactat accagtaaaa ccattcctac
     5701 ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa aatgcatata acggatcaca
     5761 caacttcaac aattctgctc ttgggaaaaa ttgatgaatt tggtgagtaa aggatggaat
     5821 aaacagcatt ccaacaatat aggcaacaat agtgatactt gtttttttgg taaaaatcag
     5881 taagcaatag ccaatttcaa tcattccgag atataaaggt atcaatagaa tgatttgggc
     5941 tagtattctt aaaaattaac tagcaaatgt ttaaactgat ttatttctaa tctacatgta
     6001 gtaagtattt caattccacc aattatcaat aaaaatacca aacatatcca taataatttt
     6061 ttctttttca tttcttttat attgttataa cagtgaataa cataaaaatt cccgacaatc
     6121 attgtaatat ttttttgtca cctgtattag tatttaattt ataggaaact ataaaaactc
     6181 cctagtgaat ttacaccttt gggagttcct gtttggacgg tatgcataat acatttaaat
     6241 caatttaaca ttaacacctc agcatatggt atctccacat atatacggtc tttctttgga
     6301 tagtactcaa tatttatgtt gatagcactt ccattgtcta atatagcttt agcccggtat
     6361 gtgttatctc cattttcatg agtaatcgtc acatcatcac atgatcgatt taagttgtac
     6421 tgttcttgta aaatctgtgt taccaatggt tttgatgact tctctaatcc agatgtacct
     6481 gtcatttttg aaaaaataat acctataata gccaagacaa caatacctat aatgacaaac
     6541 ttcttgttca tatttacttt attcaacttt ttatctccta tctccaaaat aatttgttct
     6601 tatccctctt atcagtacat tgacatccct aaaagtacac caaagattgc tccagctacc
     6661 cctgctatca tcaaaatcgt acccattttc tcatcttttt ctcgatataa gtagcccata
     6721 ataacagcaa cagcaccaaa tacaacagga ataaatgcta acgatactac catagaaatc
     6781 caaccaataa tcaaatacag cttccctgtc tcttgtccat gtacgatttg ttccttcatt
     6841 tcattaattg tctgctcagt acttttcttt actgtgtctg ttccggttgt ctcatctgtt
     6901 tcttgtagtg ctccacaaaa ctggcaatac ttactttcat ctgttatttc tttgccacat
     6961 tttctacaat acatgtcttc tcctcctaaa atcattcaat tgcttattag ttaactgttt
     7021 actttattaa atacatcaga aaacagctca ttcattggta cacttaatat ctcagaaagc
     7081 atcttcattt gatagggttt aggaatcgtt ctccctgttt gccacgaact tacacattgc
     7141 tgtgtaacat tcattttttt tgctaactcc gtttgagaaa gtcctttttc ttttctgtat
     7201 cggctgagat tacttacttt tttcatgatt ctcccctcct gttttattat atacaacaat
     7261 atgatgtatt tcaactcata aaatataaaa aacttcaaat accattacaa atacaacaac
     7321 ttgttgtact atactgagat gggggtgtat taatgaaaca gaaacaatat gcaaaggtca
     7381 ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga attagctgaa aaaatcttct
     7441 ctacacaaca ggcagtagca agatgggaaa attctgtaac agagcctaac ctcgatagct
     7501 taacagctct ttctagagcc ctaggaaccc ctgtaagcca ttttctagac aatgttgtcg
     7561 tagattatga ggaagagttt ttggctctct atcgttctct tagcactgaa gatgcagttc
     7621 gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa tgaacgcaat cagctattga
     7681 aagattgaaa aataaataac ccaccgaact ttaacggtgg gtgtattgag atgaaataag
     7741 attaagagct atggataacc tgtctagttt aatacaattg ggtcttttct cttccttact
     7801 ctccgtccat catctcttgt tacacttgta aattgataca gaggaactat cccagctcct
     7861 gataatatca attcttttat tgtttcatct atccctgccc ctttcgctaa atatattaaa
     7921 aaactactac ttgctttcac attgtatagt ttcttaatat ccttagaagc tcgttcaaga
     7981 gtgaatcctt gtttaacaaa gtagcctctg ttttttatta aagcattttt cacgctgtta
     8041 tccccaacat tacgtaggcg ttcacaaaca gtgttaataa gttctttctc tgtcattaga
     8101 tatgtccctt gccacgataa gccctgtaaa acttcatgtg cttcatctaa aatcaattgt
     8161 ttttccttgc tgagttctga tcgggaattt tgaggaaata ctcttttagc ctctttttcg
     8221 ttaaaacata tcttaaccca ttcataagaa aaatattgat atgtaaaatg aattttttcc
     8281 agaagtggaa ggatatcttc attcaatttt tgaatcacag aagttgcatt aaaaacgtct
     8341 aatcgataga cattgcttct cttatgatta aagtttccta acgctttctc ttcctttaac
     8401 ctatttaggt attcgagcat cttcaaagga acatcttcat catctaattt ttttatcaat
     8461 cctataaaag tcataagtga tagtaactta ttacatttat ctatgtcata agttttacca
     8521 tagtaagaac tcattttttt agctaattgt tcccccgaca aaatacaagt cacactttcc
     8581 ttactatcat caaaaaaatt aacatcagaa agcaagacat catagacacg tcgcatttcc
     8641 tctagataat tccttattgt cctgaaaagt ttgtctttca ttgattcaag agtgtcaaaa
     8701 agtacttttg attcctcttt caattttcga aaatgtgtag gatacaagtc taggttctct
     8761 aatagccaat taaatgtttc tacccgactg tctttcttta aaattagcat cacaactcca
     8821 ataaaatttt cactgatatc acaattactg gaatgacaat aaaaaatgga atgcccagtt
     8881 ttactggtaa aaatactagc agagggatta tgatcgttgt gaaacagaca tcttaagttt
     8941 ttacttgact taagtaattt attcatatca atggttaaca atctttcata aatctcatta
     9001 aaagacatgt cggaggaatc aaacaggcag tctttaaacc actgtttcat ttcttctttg
     9061 tcgtcatttt ttatcaactt aacaaaatcg gtttcattga ctagttcata ctctctccct
     9121 gaatcacttt gacaattttt agaacatgtc atggtaaaat caatcggaag ctcagtaata
     9181 tctaacatgt tgtcttcatt aattattatc actgcatctg acttatatcc tccaaaaaac
     9241 attctattcg aagcatttac attgtcatca cacccgggga tgttcttttt caagtattcg
     9301 taaacagcaa ccattttacc atagtcagta attgcttctt ttaatagaaa gacaatcttg
     9361 tatttttcta gattttcaga gtaccgaata cttttttgta tcgcaaacgc agaattatga
     9421 actaaattga tagataaaac cttttctatg cttaagtatc tactatcatc tttactcaaa
     9481 tctactacct tccccttctc atctttaaca gtgttatcga catccagaat aattactctg
     9541 gtttccacaa aactctcctt gtcattgcct ttattctttg tcttgcttgg aataaatgat
     9601 tgtgatgaat ttagtatctc tacaaactca tctcttgtaa cttctctata gttatctaaa
     9661 cacctatctc ttacatcagt tttttctttt tttgtaactg gcttctctaa taatttttct
     9721 tgatctacat aaattggaat attaatcatt gatgtttcca tttgtgtttc ctccttataa
     9781 aattctgtta aagactaaaa catacagata ataaaaacaa actctttatt actatatgtt
     9841 ttagtcttat ttgtttactc aacctttata ccgttccatg aaactatcaa tatcagattt
     9901 tttaaatcta atgaccgtat caatttttat aacaggtaac ccgtattgag aaacatattt
     9961 tctaatcgtt cctactgata cacctagata ttcacttgcc tgctgatagt ccatatactc
    10021 tagagtgact ccagaaataa tgtcttctag cataggcttt agcactgtaa ttatctcttt
    10081 cacttgttgt tcaacttgta tagaaacaat gctatcaatg atttgtagag acatatttcc
    10141 ccctcctttc gtgattgctt gaacaaattt tctaagaatt atcttcgtat tttttgctca
    10201 aaataaaagt attttgttat taaccgatta atcaaacaaa aaatgattac cagttcatcg
    10261 acatacacat aatacctcga taaaaaaggg gtgtcaatag ttttgtaaaa tttatttaca
    10321 acaccccttt tttgaggtat actaatttta ggaggtgtgg tcaatatgat aaaacgtcca
    10381 ccaataaatt atttagaacg aaaaaagata ctcggaacaa aaataaaagc tataagaaag
    10441 agtaaaaaat taacacaacc agcatttggt cttatgatta acaacggtca attaattgac
    10501 aaaaagacta tctacgaatg ggagaaggga acctatttac caataccaga gcgattatcc
    10561 cgaattgctg atttgggaaa tatgagtata gaggagttag tttgtggcaa tgtagaagaa
    10621 tatattttgg gaattattct ttatagagat agcattgtac tagatggtat aacatttcca
    10681 gataaaaacc tgttccagca tcttcgtcaa cagttcccac cagtacactc taatttagat
    10741 acatggctgg atagatattc caaattagaa ccagagatgc aagaatttat tgctaacaag
    10801 acttgtaata aagttaaaaa tgaaaaaatt agtcttttca atatcttaaa aatagaagaa
    10861 ttattcatca atgctattgt agaagaattt gacaataaca ttcttttcct cactagcagt
    10921 atagaagaac tactagaaag aatggtagat gaatggctac ctattcaatt gaaagacatg
    10981 tcttatcccg aagaagctgt gagagaaatt actgataata taaacaagct agaacaaacg
    11041 atttcatcaa ttgggaaaaa atacactaag aaaaaaatga agggaggtga taccatctag
    11101 tggtaagtac tccttgtaaa ctaagaggag atgatttcat ggcaaaaata tcaaagtaca
    11161 aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca cattgccacc gatccacgaa
    11221 ctggtgaaaa aataaatact acgagaagag gttttagtag caaacaagaa gctcaaaaag
    11281 attatgacga gtacagacat caaatattat acggtgttaa aaagaaagca cctgatatga
    11341 catttgaaga cctgtataat gaatggataa ctcatcagcg taccagtgtt aaagcttcaa
    11401 caatcgcaat ctcagtaaga tacgcaaata atcaaatact tccagcattt ggtaagctta
    11461 aactatcgaa tatctctgtc ccatattgtc aaaaagtggt agatgaatgg cacagtaaat
    11521 atgaaagtta cgattatatg agaaaacaga ctgcacaaat tttacgctac ggggttgcaa
    11581 tgcaatatat tgacaataat cctatggaaa aaacacttct tccacgaaaa aaagagtatg
    11641 agaaaaatcg taaattttac tcaaaagagg agcttaataa tttactggat gcttttaaag
    11701 attttggtaa catgaaacaa tacgccttct tcagattatt ggcatacaca ggtatgagaa
    11761 agagtgaagt actcgcttta cagtggaaag acatagacac gtttaataaa gaactacatg
    11821 tcaataaaac tcttgctgta gatgaatttg gtaaagttat aattcaatct ccgaaaacaa
    11881 gagcttctag acgagttatc tctttagata ctgagactct ttctatcctc aacaactgga
    11941 aattacagca aaaagaagag tatttaaaac ttggatacaa tactagctct aaagagcaac
    12001 atgtttttac aacagttaaa aacactctat acattccaaa cactgttaat gattggttaa
    12061 gatatatttt gaaaaaatat aacttgcctc gtattacacc tcatgggttt agacatacac
    12121 atgctagttt attattagaa gcaggagaat ctgtcaaggt agttcaacaa cgattagggc
    12181 atgaaaatag taaagtgaca ttagacatct atgcacatat tacaaataat gcacctaaga
    12241 aaacaggtca agactttgct gatatgatgg ctcatcagta aaagagctgg tcaaaatctg
    12301 gtcatttcta gtatacaggc aaaacaaaaa agctagaacc cttatatatc aagggttcta
    12361 gctcctataa caggtttacc caacactgcc ttccatctca taactgatca atcgattgag
    12421 ttcgactgca tattccattg gaagttcttt ggtgaatggt tcaacaaagc ccatgacgat
    12481 catttcagtt gcttcagatt cggtcaggcc tcggctcatc agatagtaga gttgttcttc
    12541 tgagattttt gagactttgg cttcgtgttc taaagagact tggctgttgt ggatctcatt
    12601 gaatgggatc gtatcggatt tcgaccattc gtccatgatg atcgtgtcac attcgatatg
    12661 agagatcgaa cccgcactgt ttttggcaaa ggttacttgt cctcgatagt ttacttctcc
    12721 gccgtctttt gaaattgatt tagagacgat agagctagat gtatttggtg cattgtggat
    12781 catttttgca cccgtatctt ggatttggtt tgctcctgca aaggcgatgg agagcatggt
    12841 gcctcgtgca ccttttccat ctaagtaaac acttgggtat ttcatcgttg tttttgcacc
    12901 taggtttccg tcgatccatt caacggttgc gccttcgtat gcttttgctc gtttggttac
    12961 taagttgtac acattgtctg accagttttg gatcgttgtg taacggcaat acgcatcttt
    13021 tcttgtaaag atttctacga tggcggcatg caggctgtta cttgaataag taggtgccgt
    13081 acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca atgatcagcg tacgttcgaa
    13141 ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt aatggaacat cgactttgac
    13201 gccttttggc acgtaaataa aggtcccacc tgaccatacg gctgaattca gcgctgctaa
    13261 tttgttgtcg gttggcggta caagttttga aaagtactct ttgaataatt cagggtattc
    13321 tttcaaagca gagtcagtgt cagtaaagac gattcctaac ttttcgaatt catctttcat
    13381 attatggtac acgacttctg attcatattg tgcggaagca ccggctagat aagctcgttc
    13441 agcttctggt ataccaatct tttcaaatgt atctttgatt ttttctggta cgtcttccca
    13501 gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg atggcatcga aatcgatgtc
    13561 tgaaaggtcg gggccccatg tttgcattgg cattttgtta aatgtttcta gagatttcag
    13621 acggaattca agcatccatt ctggttcgcc tttgattctt gacatctcac ggatcacttc
    13681 ttctgtcaag ccttctcctg tactgaaaac cggttcgaca tcatcgtgaa acccgaattt
    13741 atattcttct aattctggta cgcccatgtt ctcactcctc ttccttgtgg taatgatgga
    13801 aagctgcggt accatcgttt tccattgctt ttcctagtgc tttccaggca agtgtcgcac
    13861 atttgatccg agcgggaaat ttagctacac caccaagcat agcagcatcg cctaattggt
    13921 cgggatcttt gacttctttt ccttggacaa gctgtagaaa gtctacagtt aaatgttctg
    13981 cttcttctat tgattttccg atgacggcgt ctgtcatcat cgaggcactt gccgtactga
    14041 tggagcagcc actgccgtca aaggcgatat ttttgatgat tccgtctttt acatcgactt
    14101 gcaattcgat cacatctcca catgtggggt tgttcaattc gatcttgctt gtcgagtcag
    14161 tcaagcttcc acgatgatgc ggatgggaag aatgatccaa gatcacttga cggtataaat
    14221 tgtctaattt agatagtccc atgttggaaa aactcctttg ttgccatgat tgcttcaact
    14281 aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc tcgcgcgagc agtcgctgat
    14341 acttcaaggt acttcaatag cggttgcgca caatgatgac ctgctctgac ggctacccct
    14401 tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc catccaaatt gaatgcaacg
    14461 actcctgtcc gaaaattcgg gtcttgtgga ccataaacag tcaatccatc aattgcttgt
    14521 aattttggca acacataagc ggctagttct tcttcatatc gatggatatt gtccataccg
    14581 agtttttgca aataatcgat tgcatggcct aatgcgatag cgccggcgat atttggtgtc
    14641 cctgcttcga atttccaagg tagttctttc catgtactgt cgtataattc gacaaaatcg
    14701 atcatttcgc caccaaattc aacaggttcc atttcttcta accattttcg tttaccatat
    14761 aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc tgaaagcaaa gaaatcacag
    14821 tctagtgctt ggacatcaat ggccatgtgc ggggcagatt gagcaccatc tactacgatg
    14881 accccgccgt tttcatggac aagttcagct aattcttgaa caggattgaa gacacctaag
    14941 acattggata catgagcaat ggagacgatt ttggttttgt ccgtaatctt tttcgctgca
    15001 tcctccatat ccaggaagcc ttcttctgtt agttcgatat atttcaagac agcacctgtt
    15061 cgttttgcca actgctgcca cgggataaca ttagaatgat gttccatgta agagagcact
    15121 atctcgtctc ctgctttgat atagcggtcg ccgaaggttc tggctaccca attcaagctt
    15181 gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt ttgcatggat gaacgcccgt
    15241 actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt ctgctaacgt atggacaccg
    15301 cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta atgcctggag tacttgtttt
    15361 ggcttttggg tcgtcgctgc attatccaga taaactaaag gttcatcatt gacgtcttga
    15421 aataagatag gaaaatcatt gcgtagcttt ttagcatcga tcatccattc aacttccctt
    15481 cgattacttc gactaattct ttccgaactt gttctacagg gattgctgtt aagacagaac
    15541 cgaggaatcc tcgaatcact aagcgctctg cttcttcttt atgcaatcct ctactcatca
    15601 gatagtacat ttcttctgga tcaactcgtc ctacactggc agcatgtcca gcagttactt
    15661 cgttttcatc gatcaaaaga ataggattcg catcaccacg tgctttatca gaaagcatca
    15721 agacacgact ttcttgttgg gcatctgcac cttttgcccc tttcaagatg tggccgatac
    15781 cattaaaggt caatgttcct ttttctcgga tcactccgtg ttgcaaaata tgcccgatcg
    15841 tatgaggggc tttattggtt actcgtgtat cgattccttg gatttgtctg cccgagctga
    15901 ttgccacgat tttgacttct gcgtgtgcac cttctcctgc taaatctgag tcaaagtcag
    15961 cgatcacatg accgtcattc atgacaccaa ttgcccagtc gacagaagca tctcgtaaaa
    16021 tgtgcccacg gcgattcata taagaagtaa tattttctcc taattgatct acggctgaat
    16081 atttgatttt tgaaccagct ttagcgatga cttccacgat gatattacct gatgattttg
    16141 ctacttgttc tttggttgtc tggaaacgtt ccagataaga aaattcacta tgttcatctg
    16201 ccacgatcaa cacatgcttg aagaagtgct catcggatgc gccatcttga atgaataagg
    16261 actcgagtgg ttcatcgatc acgacatttt taggcacata caggaaaata ccgctgttca
    16321 taaaagctac atggagtgca gtcaattggt cttcgttcat gtttactgct tttttcatat
    16381 aatattcttg gactagttct ggatattcga tcattgcagt gaacaagtcg gtaaaaatga
    16441 ctcctttttc tgctaattcc acaggcagtt gttcaaaaat cgtccatgaa ccttgttgga
    16501 caagcacagg attgtctttc attgcatcaa atgccggaat cgtgcctgtt tcactgatgg
    16561 tttgtgtttc tttcacatca aataacgacc agcgatggaa tttcacccga tcgatatgcg
    16621 gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg gaaggttctc atccaatcag
    16681 gttcgccttt acttaaagaa aaatcattta ccgcgtcaag ataatcgagc cagttttttt
    16741 ctttcattct tgctccccct tacgcttctt cttctttata gtcgattcct aattctttac
    16801 tgataccagc gtagccttct gcttccagac gtttagctag gtcagcattc cctgtcatga
    16861 ctactcggcc ttccatcatg atgtgaacga catcaggtgt gatatagttc aataatcgtt
    16921 gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg catttcattt actcctttag
    16981 caacgacttt caaagcatcg atatccagac ctgaatcgat ttcgtctaaa attgcaaatg
    17041 taggttctaa catcaacaat tgcaggatct cattccgttt cttttctccg ccagaaaaac
    17101 cttcgttcag ataacgttca gccatttctt ctggcatatt tagtaaagcc attttttcat
    17161 ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt ttcatcacgt ttcgcattga
    17221 tcgctgcacg catgaattcc gcattcgtga tacctggtat ctcacttgga tattgcatag
    17281 ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa gtctaaaaca tttttcccgt
    17341 caaacaaaat ttcgccttct gttacttcat agtttggatt gcccatgata gcggcagata
    17401 aggtagattt tcctgtgccg ttaggtccca tgatggcatg gatttctccg gttttcattg
    17461 ttaggtttac gcctttaagg atttcttttt cctcgatcga cacatgtaga ttttttattt
    17521 ctaagacaga catgcattat acccccaaat tctctttcgt tctcactcat tttagactaa
    17581 ttgagaatga agcgcaatga ccttccttac taaaatcaaa tatcttttat ttattattaa
    17641 acaattttga tttctaaatt agaataggta taaaaaagat gagaaataag ccattacaga
    17701 cttatttctc atcttttttg ttccatacct tattttgcat cagcctttac tggtttaaca
    17761 gagccgcccc atttgtctgt gatccattct tggacttctt tgcggtgaag cacttcaatc
    17821 agttttttga ttttgtcact gtcttcatca cctttacgag tcgcaataat attcacgtaa
    17881 ggagagttgt ccttttcaag caatacggca tcttctaatg gattcaaccc tgcgccgtaa
    17941 gcgaagtttg cgttgattgc aactaggtca ccttcatcat tttggtacgt agaagctaaa
    18001 agtgatgggt cgatcgtatg gttgaacttc aagttcttag ggttttcttc aatatcatcg
    18061 aatgttgctg tttccagatc tactccatct ttgactttga ctaagccagc atcttgtaga
    18121 attgtgatga tgcgtcccca gtctgattca gaatttgaag taatgattgt tgcaccatct
    18181 tttaattcgg aaacatcttt gactttttta gagtacaagc ccattggttc gatgtggaca
    18241 gcaccggcat tgacaaagtc atatcctttt tctttgattt ctttgttcaa ataaggaata
    18301 tgctggaagt aattggcatc aatgtcgcct tcttccaaag attggttcgg taatacatag
    18361 tcatcgaatt tgacgatttc aagatcgatt ccttcatctt tcaataatgg tttcacatgt
    18421 tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt tcaatgtcgt gtcgtctgct
    18481 ttatttgctg aatctttgct gtcgctgttg ttaccgcatg ctgctaatcc tacagtcagc
    18541 aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat cctacatccc cctatatcct
    18601 attatctttt gtctgttttc ttagttaaga aatcaccgat tccttgaacg ataaatacga
    18661 ttagtaaaat aattaaagtc gcaacgaatg tcactgttag attaccacgc tggtagcctt
    18721 cctgccaagc aagacctcca agtccacctg cgccgatcgc gccggccatc gcagtaaatc
    18781 cgatcatcga gatcgttgta actgttaatc cagaaatcaa tgcgggaata ctttcaggta
    18841 tcaagacctt ccagatgatt tcccagtaac ttgctcccat tgcatctgct gcttccagta
    18901 caccagggct gacttcacga aaagcaatct ctactagacg ggcataaaat ggtgctgcgg
    18961 ataaaacaag agctggaatg gctgcttgcg cacctagcat agtgcctacg attgctttag
    19021 taaatggaat gatcaaaacc atcaggatca taaatggcgt tgaccggaaa atattactga
    19081 tgatcgatac gattccataa atgattcgag catacggttt gtttttgcgt ccaatagagt
    19141 aaaggatcaa tcccaaaact aatccgatga tcgtcactaa gaccattgag attaatgtca
    19201 tatatagtgt atcaaggata gcctgattca tcgtttccgg gttgatttgt ttaaaatcta
    19261 gatatgtttc tgaaaagctt ttatccacga tggatcacct caattcctac tccttgttct
    19321 ttgatgaaca cttctgcttg atctaattct ttggtttcac cagtcatcaa gacagtcaat
    19381 gaaccaaagg aaccttcttt cacttgtttg atctttccat acacaacgtt gacatcgatg
    19441 ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg catttctttc gctaaaggtc
    19501 aaagttgcca aacggccgct tggattttct ttgatgagtt cagctaatag ttctgtcgaa
    19561 tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg ttacttcttt tttcggttgt
    19621 ctgaagacag tcaatacgtc tccttcttct acgactttcc ccaactccat cacagcgact
    19681 ttgttgcaga ttttgcggat cacattcatt tcatgtgtga tcaagacgat ggtcaaattc
    19741 aatttcttat tgatcgctaa caataagtct aatacttctt ctgttgtttg cgggtccaat
    19801 gcgctagtag cctcatcaca aagcaggatc tcaggatcat ttgccaatgc tcgtgcgata
    19861 ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat atgcttgtcc gcgtccttct
    19921 aacccgacta aacgcaacag ttcttctgcc cgttctttac gtttgctctt tggtacgcca
    19981 gctaattcca gcggcagctg gatattttca agtacagttc ttgaccataa caggttgaaa
    20041 tgctggaaga tcataccgat tttctttcgg aatgtacgta attctttgtt ttttaatttt
    20101 gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg gaagctcaag tccattcagg
    20161 agacggacta atgtactttt tcctgcgcca gaatagccga cgattccata aatatcccct
    20221 ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt ttccgttttt cccattgaat
    20281 gtctttttga cattcgttag ttggattaat gacatgccat tcacatcttt ctcttataat
    20341 ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa aaggacgaaa gcttgaactt
    20401 ccgtgttacc accttgattt accgttactt cgcaataaca gcctcttcca gtacttggac
    20461 ttagcttctt tccgtatact gtcgcgctgt aacgggcact cccgtagtaa gtttgcttac
    20521 gctcgcttac tctgctctaa gaccatcttc catctcattt tcgttaccca ttttcagcaa
    20581 acagggctct cttcaaacgt ccgtgacatg tactcttctt ttcaaagctt tctcgtttca
    20641 tataggattg cttatgatta tagcaactgg aagaatcagt gtcaactttt ttcaagtgat
    20701 tttctgattt tataattcgt caaattcttt tggatcaaca tctttgaaac ttaagatcca
    20761 cgcattcatt tcatctgaat cattcaagat attgatatct tcaccgattt tatcattaac
    20821 agaagaaact gtacccgtta gcgggctggc aaattcattg accgcttttt ccgcttcaac
    20881 ttcgatcaga ggttcatctt tttttagcga ttgtcctgct tttggcagat tggcaaacgt
    20941 gatgtctcca agttcttctt gcgcttcatt tgtcaaacca acacagtatt cttttccatt
    21001 aaataagatc caaaggttgt cctttttctt caaatgcttg cctgccattt tatatcagtt
    21061 ctccttcata aattttttca ttgaatccaa ttgcaacaag ctggtcctct cgtaagatca
    21121 gtggtcgctt gatcagcata ccatctgtcg ataagacttg gctcgctttt gaagccgtaa
    21181 actcgcctac ttgatctttc agtccaagtt cccgatattt catcccgctt gtgttgaaca
    21241 ggcgacggat tggtacttga ctcgtttcta gccaagtctc aatcgttgag gccttcggta
    21301 cttctttgat catatcaacg gtttgataac taacgccatg ctggtctaac cacgctttgg
    21361 cttttttgca tgttgaacat ttcgggtacc agtaaaaggt gtacaatttt cttacctcct
    21421 tgaagatctt gcaggtgctt cattttggta tctcatcgat aatatcaacc cgataccgat
    21481 catgttccct aaaatcgagg atcctccctg actaatgaaa ggcaatggga tccctgttaa
    21541 aggaagtaat ccgatatttg ctccaatatt ctcaaagaca tggaacagca gcatcatgat
    21601 caaaccgctt gcgatatagg cgtaaaattc attattcgta tcgaaacaca cccgaatcat
    21661 acgatagatt aaaataaaat acaagaaaat gacgaatgca ccaccgataa agccgaagtt
    21721 ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca ggcacataga tattgcttac
    21781 gttgaatcct ttaccaaaca agccaccggt accaattgcc aaaagtcctt gggccggctg
    21841 ataactcatt cctgacgtat catggaaagg gtccagccat gaatcaatac gtgcaaactg
    21901 ataagatttg aatccaactt tataaagcaa gtctcttccg ccttcagtcg taaccaagaa
    21961 gatcgttcct gctccaacga ctactgccaa gattactaca ggtgcgatga tgcgccaaga
    22021 gattccagac atcaggaaaa tcccgccaaa gatcgcaaga aagacaagca tcgttccaaa
    22081 gtcatcttgg gccatgatca atgctaaaac cggtattgtc acgatcaaga gcttcccaat
    22141 cagccaaaag tccgatttca tcgtccgttc ttttacttgc gtattatgtt tagttacgat
    22201 cagtgccatc atcaagatgt acgcaatctt catcaactct gctggctgga aagtaaacgc
    22261 accgaatcga aaccagtttt tagaccctgt tgaagcagct aaattctgat catagaattt
    22321 gagcaagagt cccattacca ctaaccccaa accataaagg taaggagtca gtttccagat
    22381 ccatttggaa ttcaaatgca tgatgacaac gattgctgcc gctccaacta aataccagac
    22441 aacttgcatc cctacgcctt tcaagactgc tggattgttc ggatcatggc taagtgctac
    22501 atataatgag agcagaccaa tcaaacataa cagaaagact ggtaaaatca cgccataatc
    22561 aatacgatta tcgttcgaag cttttaattt tttttccatg tttcatccct tttcttaaaa
    22621 accatactgc tgattattat agcggtccag ttagataatg gaaagtcttt ccatagacat
    22681 ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc aaatgcagta caaaagccta
    22741 ctgcttatct ttagtgtatc atagaacttt ttttgataca tgcaattatc taccagctga
    22801 gattctcctc gctgtgagaa gtttctgtgc tttcattgta tcgaaggatc tcttcggcaa
    22861 tctctagcag ctgatcagaa accagattgg ttcgttggat gaaataaaaa tatcgatgga
    22921 aattcaacgg agtcattggc acattttctg gaacagagcg cttagacaaa atcgagcgac
    22981 cgattccttc cttcagcaac gcaacaatta catcgttact tttcacgatc atcttcttat
    23041 cttggatatt catctcttca aaataacgct gcgtgtaatg aaaaacacca gagtttgctt
    23101 ctcttacaag ccacaatttg ctttttgggt ccccagcgat gactagctgg tcttccatca
    23161 aagagtatct ttgtacgccg gaagtctcta atggcttttc gataaaacca aaatcggatt
    23221 catgtttcgc tacttgttct aatgattcat gagaattcgt gatttcgatc tcaaaatgca
    23281 ctttaggaaa ccttttgatc agatgtttca tcaactgagg caaaagatag acagcaaagg
    23341 tgtgggaacc aacgatccga catacttctt cttcattttg ttcattgagc aatttttgct
    23401 gagtttcttc ccattcttct aacaaaacca gtgtccgttc atataataag tcggcttgct
    23461 tagttggaag catctctttt cttccgttac gttgaaaaag atcgatattc aattcccgct
    23521 ccaactgttt gatttgattc gagactgctg gttgagataa aaacagtact tctgctgctc
    23581 tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt taataactta aacatggcac
    23641 cacttccata actttttatg ataattacta taaaaactat ttgatttaaa tatacaaaaa
    23701 accatctata atagaaatgt acaacaaaac agttgctgat ccaactagta acttagatga
    23761 agacaatcag ctgtctgctt gctcgtaatc atacattcct attcatttta caatgtattc
    23821 gttcatcttg gtactaaaat ctattaaata aaaaggagaa tattatatat gaagaacttt
    23881 ttgaaaatta ttccgatccc gatctgcggg ttgatccttg ggttagcttc ccttggaaat
    23941 ctgtttaaag actatcacca tgttgcttta ggcaatctaa ctggcggtat ctctatgatt
    24001 cttatgatat tcatttttgt taaattgttt atcttattcg aacacacgaa acaaacgttg
    24061 catgatccaa tcatcgcctc cgtttcacca acttttacga tgtcactcat ggttatttgt
    24121 acttattttg tttcatatga aagtattgca ccaattgtca aattcatttg gctgtgcgct
    24181 gttatcttcc aagtgatcct tgtcttttat ttcaattatc accatgttat caaagctgat
    24241 ctttcaattg aagcaattta tccaagctgg tttatcattt acgttggatt tggggtcatc
    24301 actgtaactg ccgggaactt cttcccactt attggaaaaa ttttcttttg gatttcattg
    24361 atctgctatg cgttcttatt gccgatcatc atcaaacgtg tctttttcgt aagaaatatg
    24421 gctcatccga cacgtccgct gattacgatc gttgcagcac ctggttctct ttgcctaaca
    24481 ggttatctta aaaactttgc tgaacctaat ttttatcttg tcgtagcatt gttcgcttta
    24541 tctcaagtat tgtatttcgt tgtgctgtct atgcttccac gtttgttcaa gcttcgtttt
    24601 tatccaagct atgcagcctt tacattcccg cttgttatct ctgcaacagc tatatttact
    24661 actgttcatt acttcaacag tttaggtatc tatagtagcg tattggattt cttaaaagtt
    24721 gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc ttgctcatta cgtacgtttc
    24781 ttaattaaag aacatagcaa acatggaaaa tttgtttcta aaaaaatcgc ttaataatcg
    24841 attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacggcc aaacaaaaac
    24901 agctgttcat gtttttgtac tggttgggct tattaccgca gatgcaagct tttgaacacc
    24961 gtttaccaag aggtcgtaa
//
LOCUS       DACCSK010000033        24932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCSK010000033 DACCSK010000000
VERSION     DACCSK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.033"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(12..2156)
                     /locus_tag="IVP39_001171"
     CDS             complement(12..2156)
                     /locus_tag="IVP39_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7586252.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2513..3886)
                     /locus_tag="IVP39_001172"
     CDS             complement(2513..3886)
                     /locus_tag="IVP39_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7586253.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3886..4281)
                     /gene="rbsD"
                     /locus_tag="IVP39_001173"
     CDS             complement(3886..4281)
                     /gene="rbsD"
                     /locus_tag="IVP39_001173"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7586254.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4309..5226)
                     /gene="rbsK"
                     /locus_tag="IVP39_001174"
     CDS             complement(4309..5226)
                     /gene="rbsK"
                     /locus_tag="IVP39_001174"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7586255.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5334..6347)
                     /locus_tag="IVP39_001175"
     CDS             complement(5334..6347)
                     /locus_tag="IVP39_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7586256.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6428..7027)
                     /locus_tag="IVP39_001176"
     CDS             complement(6428..7027)
                     /locus_tag="IVP39_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7586257.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7213..7914
                     /locus_tag="IVP39_001177"
     CDS             7213..7914
                     /locus_tag="IVP39_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586258.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7924..10635
                     /locus_tag="IVP39_001178"
     CDS             7924..10635
                     /locus_tag="IVP39_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7586259.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11086..12714
                     /gene="treC"
                     /locus_tag="IVP39_001179"
     CDS             11086..12714
                     /gene="treC"
                     /locus_tag="IVP39_001179"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7586260.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12774..13955)
                     /locus_tag="IVP39_001180"
     CDS             complement(12774..13955)
                     /locus_tag="IVP39_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7586261.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14207..15730
                     /locus_tag="IVP39_001181"
     CDS             14207..15730
                     /locus_tag="IVP39_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7586262.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15799..16527)
                     /locus_tag="IVP39_001182"
     CDS             complement(15799..16527)
                     /locus_tag="IVP39_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586263.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16540..17820)
                     /locus_tag="IVP39_001183"
     CDS             complement(16540..17820)
                     /locus_tag="IVP39_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7586264.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17961..18563)
                     /locus_tag="IVP39_001184"
     CDS             complement(17961..18563)
                     /locus_tag="IVP39_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7586265.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18808..19014
                     /locus_tag="IVP39_001185"
     CDS             18808..19014
                     /locus_tag="IVP39_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586266.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19326..20045
                     /locus_tag="IVP39_001186"
     CDS             19326..20045
                     /locus_tag="IVP39_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7586267.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20126..20809)
                     /locus_tag="IVP39_001187"
     CDS             complement(20126..20809)
                     /locus_tag="IVP39_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7586268.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21027..23213
                     /locus_tag="IVP39_001188"
     CDS             21027..23213
                     /locus_tag="IVP39_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7586269.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23559..23969
                     /locus_tag="IVP39_001189"
     CDS             23559..23969
                     /locus_tag="IVP39_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7586270.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            23983..24339
                     /locus_tag="IVP39_001190"
     CDS             23983..24339
                     /locus_tag="IVP39_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7586271.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8350 a         4427 c         4863 g         7292 t
ORIGIN      
        1 tttaatttat tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac
       61 aaggttagta caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt
      121 cttgccgtaa aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg
      181 ttgttgacgc gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc
      241 catttgattg gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca
      301 gttgttgctg cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc
      361 gcgttcaatt tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt
      421 gcttctttgt agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca
      481 gtcagtccat ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat
      541 ttacggaatt gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg
      601 aaggtcacgc tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg
      661 cttgataata agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat
      721 gcgtagattc cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaat
      781 tgatcagttg tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat
      841 gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca
      901 aaatcaggcg tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca
      961 tggataaatt gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt
     1021 ccccagaaga cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat
     1081 tgctgttcgc ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa
     1141 gtcaaaccag aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca
     1201 agatgcttga atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat
     1261 acagcatcaa ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata
     1321 ccgctttgat ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa
     1381 ccattgtttg gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg
     1441 tttgtttgat tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt
     1501 gaaccattat tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt
     1561 gtactaggga cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct
     1621 ttaaattctg cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa
     1681 tttacttctt tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta
     1741 atagtgtaag ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata
     1801 tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc
     1861 aaagaaggag caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca
     1921 gccggatcaa tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg
     1981 tcttctgtaa aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt
     2041 gtgactgttt gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca
     2101 gacggagcaa tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata
     2161 aactagtcct tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt
     2221 ggaacagata atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa
     2281 taaaacaatt tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat
     2341 tgcatagacc acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt
     2401 aaattatacc aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag
     2461 aaaaagactg aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat
     2521 aagacattag gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac
     2581 taacagccag tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg
     2641 tcagactacc aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac
     2701 cagcagctgc accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt
     2761 aaacagttgg ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg
     2821 aacgggtttt tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa
     2881 aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga
     2941 tcatgacagg gtttacagcc cattggatac caaatattac caaaatattc caaccaccag
     3001 cgatgattgc gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta
     3061 aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga
     3121 taactaatga aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga
     3181 ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg
     3241 tatactgcat cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg
     3301 aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc
     3361 cattcacttt aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga
     3421 cggcattcga cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa
     3481 tcgtagtcgc taaaacaacc agacctttta atataccaga accggcaaac agcggaatga
     3541 tcggcgtaaa gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt
     3601 tcggagctga atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat
     3661 ccatgattat ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct
     3721 gttttaattt ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg
     3781 cacatggaag gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa
     3841 tctgctcgga cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc
     3901 actcactaat ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca
     3961 gttattcaac tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa
     4021 caataatttg attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc
     4081 aatcgattgt acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag
     4141 accgtttgag acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc
     4201 cccgatactt aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt
     4261 gattactttt ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga
     4321 ttgttctaaa gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac
     4381 agaagaggct agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag
     4441 tgtgctgctt aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac
     4501 tttaaaagca gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc
     4561 tagtgtaatg attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa
     4621 actgttatga tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat
     4681 aatgatatct gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt
     4741 caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa
     4801 gctactttca aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag
     4861 gacttcttgt tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc
     4921 tgctgcgtct accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc
     4981 agatgtatcg atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc
     5041 cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt
     5101 ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt
     5161 gggcatgttg gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt
     5221 gttcatttta caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa
     5281 cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc
     5341 aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct
     5401 ttttctggga attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag
     5461 attggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca
     5521 tagccaataa tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat
     5581 aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt
     5641 tctgctgtga tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg
     5701 tcgtgaggga tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca
     5761 ttttccggtg gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag
     5821 tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct
     5881 tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg
     5941 attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg
     6001 agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg
     6061 acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga
     6121 ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta
     6181 gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt
     6241 ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg
     6301 ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta
     6361 tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg
     6421 ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg
     6481 cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc
     6541 aagcatcttc agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat
     6601 atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa
     6661 gttgaagatc aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca
     6721 tattatctgt atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag
     6781 aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta
     6841 cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt
     6901 ttgtcagaat agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt
     6961 gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat
     7021 cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg
     7081 tttatttgtc aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa
     7141 ataaataagt gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat
     7201 ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga
     7261 gaaacgacca ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg
     7321 attatacttg gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg
     7381 gatacacctg atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat
     7441 aaacagctta ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc
     7501 gttcctaatc taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg
     7561 cttgatcctg tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg
     7621 tcccaattat caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat
     7681 cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa
     7741 gtattgaaat tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca
     7801 cataattctg ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta
     7861 aggtctgttg aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat
     7921 tttatgaaaa caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga
     7981 cgattcattg caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa
     8041 actgctggac ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct
     8101 gatttgcaaa tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag
     8161 atcccagatg caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac
     8221 gaagtgattc aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact
     8281 gatggaaaac tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa
     8341 gggtataaaa tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat
     8401 accatcacgg gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg
     8461 aacgtcggaa atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca
     8521 gatatttatt ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc
     8581 aaagcttaca aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat
     8641 cgacccgaag aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca
     8701 cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat
     8761 gctaaaaaac agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga
     8821 acaatcgctg ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa
     8881 ttagttgaga aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa
     8941 aaaattgaaa atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt
     9001 tttcaagaat ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt
     9061 ttctttttct ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa
     9121 aaccgcaggg aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa
     9181 aaatatgcaa tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta
     9241 ggaacgaatc ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg
     9301 agtgcgctta ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt
     9361 gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca
     9421 ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg
     9481 ctgtggtcac gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag
     9541 tcaagaatgc ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc
     9601 gtgggattga aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac
     9661 tatcaagcaa tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat
     9721 atgctgcaga agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa
     9781 ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct
     9841 gatttggatc gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa
     9901 acaggaatcg ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat
     9961 atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa
    10021 aactatctag gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa
    10081 tcgatgatgg ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa
    10141 atgctttccg agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa
    10201 caaatcgaaa cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc
    10261 gtattatcag gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc
    10321 gagcgtgtac gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg
    10381 atgtacattg ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt
    10441 gtaggatata tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt
    10501 ccattagtga tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg
    10561 gttattgtga tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg
    10621 aaatcgaatg aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa
    10681 acaaacggca aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc
    10741 tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat
    10801 actcaaataa aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa
    10861 agcttttgaa caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat
    10921 agacaaatac atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata
    10981 aagataaatt ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata
    11041 taagttgtgg taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac
    11101 aaagtcattt accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga
    11161 gatttaaaag gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt
    11221 tggctaaacc ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac
    11281 acagccatcg atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca
    11341 aagaaatacc ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat
    11401 gaatggttca aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga
    11461 gaagagccaa cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat
    11521 acggggaaat attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac
    11581 caagcagtaa gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa
    11641 ggattccgtt tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa
    11701 gaaaatgatg gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa
    11761 ttgaaccaag caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct
    11821 acgacgatcg aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc
    11881 tttaatttcc atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca
    11941 tttgattttg cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt
    12001 aacggctgga atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt
    12061 gtagctatcg atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg
    12121 aaccgtggga cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat
    12181 tcatcgattt ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa
    12241 caaggattca cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct
    12301 cgaacaccca tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg
    12361 ttaaaagttg gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat
    12421 tctattttgt cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca
    12481 caaggagatt atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa
    12541 tttgaaggac agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc
    12601 atcccagaag gattcacaga agccaatatt cttatcgata attattccga tactgttctt
    12661 catcaaagtc tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa
    12721 aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta
    12781 aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta
    12841 ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag
    12901 ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg
    12961 attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag
    13021 tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta
    13081 gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga
    13141 atgatacata acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt
    13201 aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga
    13261 aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct
    13321 ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa
    13381 gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg
    13441 ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc
    13501 acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa
    13561 aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc
    13621 tttgcctgtt cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa
    13681 ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg
    13741 atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta
    13801 gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc
    13861 agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg
    13921 atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact
    13981 tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg
    14041 tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt
    14101 aaccatatga taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg
    14161 aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat
    14221 aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa
    14281 agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt
    14341 tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga
    14401 ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt
    14461 tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga
    14521 aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga
    14581 aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca
    14641 tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat
    14701 tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa
    14761 acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt
    14821 tgtcacgcaa catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc
    14881 gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca
    14941 ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat
    15001 tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg
    15061 attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct
    15121 cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa
    15181 aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct
    15241 ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga
    15301 tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga
    15361 atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat
    15421 ggcatttacg tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga
    15481 aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa
    15541 ggaaagtggc gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc
    15601 cgaacatttt catttatatg caggaaatga aagtcaagaa gtattgctga aagaaatgga
    15661 gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac aggaaatgct
    15721 tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctggtcat ttagtcggtc
    15781 caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag aggctgggat
    15841 ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc gcacattttg
    15901 tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt tctcgatttc
    15961 tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca gtttatgtgg
    16021 cgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt tgatctcatc
    16081 attaaactct attttgaaga tatctttttg gatttgggac atttgaaaat cttcgaaaat
    16141 tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt tgacgatata
    16201 atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat gggaagtcac
    16261 atagattatt tttcctaaag gatctaattc acggatcaat ttaccgatcg ctacaccatc
    16321 catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag aagttctgat
    16381 cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat aactgtctaa
    16441 gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt gttttggatc
    16501 gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt gtatataaat
    16561 cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag gattggtctt
    16621 tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg tttttgttga
    16681 gaaccctttt tgtttcaact ggtttaacgg taatgtattt gttgttgtat tgatcacacg
    16741 aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct tctttgcagc
    16801 actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa ggtttaccgt
    16861 atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgatca accctttttc
    16921 ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt tcatattatc
    16981 caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg
    17041 atcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt gaagagaaaa
    17101 aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt tttctagatc
    17161 tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag ctaatttctt
    17221 attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat atagaatgaa
    17281 aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg tataaaaaat
    17341 aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac ggcggtccac
    17401 agtgtgaaac caagtctcta acgatttttt taaacaaaaa gctaataaag cagataaaaa
    17461 agcggataag acaatgtgaa aatactgcaa tgaatctcct ggttcaataa ccgaagattt
    17521 caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc
    17581 aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat
    17641 cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga cccatccaat
    17701 caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga tgtgatggtc
    17761 aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc ataaaaacat
    17821 aaagtacagc tcccattgat ttttaccaaa ttatagcaca attggcgtat tattttttta
    17881 atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat acaaaaaagt
    17941 cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc tccaaaattc
    18001 atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc aagtagcaaa
    18061 taggtagaaa agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat tagaaacatg
    18121 ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt gattgctgta
    18181 cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt
    18241 gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca taaataaagt
    18301 agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc atatctgcgg
    18361 atgcagatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt caccagagaa
    18421 aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa gacaaatttt
    18481 acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc ttctaataat
    18541 ttgttcacaa ctttatcttt catctcatac gtacctccac cacaaatttt atgctaaaaa
    18601 aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc gtcatgatct
    18661 cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa
    18721 aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa aattactgac
    18781 cttataaaag gtgatggagg gattcttatg gaaaatgaac aattgtttga agtcggcgaa
    18841 acatatgcat ttaaatctga tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc
    18901 tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag tagaagacaa tgataccgct
    18961 cataatctgc actggcagtt agttgtacgc aaatcaagta caatagaaga ataaaaacag
    19021 ataaaatgtt gagagcataa agaggttgtg agtctcccgt ttagctctga gttacaagga
    19081 ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg
    19141 caggagaata ctgtttattt gaaaaaaggg ctgcgacaag acttttgtca cagcctttat
    19201 tttcatgaat aggagttact tttggctact tttagagaag tagcttttaa ggagcagata
    19261 aagtcaaggc ttttatttct ataaatgaag tagtaaaatg aagaacagat tttattacag
    19321 gaaggatgcc gataattgtg atcattgttg caggaacgat cggtgcaggg aaaagcacac
    19381 taactgaaat gcttgcccaa gatttagaaa caaaaccttt ttatgagaac gtggaagata
    19441 atgaagtatt gccgctattt tattctaatc cagaaaagta tactttttta ttacagattt
    19501 tctttttgaa caaacgcttt ttggcaatca aagatgcttt cagccatgat gacaacgtac
    19561 tagaccgctc catctacgaa gatagtatgc tgtttcattt gaatgcagat ttagggcgtg
    19621 tttctgaagt agaagtaaag cagtatgaag gtcttttaga aacgatgctg aaggagttag
    19681 aagaaatttc gccacaaaag aaaccagatc tactggttta tattcgtgta tcctttgaaa
    19741 ctatgttagc gagaatcaaa aaaagaggcc gggaatatga acagctagaa caagatcctg
    19801 aactctattc ctactataaa gaactcaatc gacgatacga agagtggtat gaacagtttg
    19861 atatatgtcc caaaatagtg atcgatgggg acaaatatga tttcgtagcc gatccggcat
    19921 gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag
    19981 atgcccagtc gatccgctac actacaaata acccgaaagc aaagggaatc tttacaacta
    20041 aataatataa aaactaagca aattgctcac tcgcagatat ttctctgtga gcagcatctt
    20101 gcttagtttt tttgattcat tgagatcaaa tagtgaaccg attcgtatga taaaattgcc
    20161 ataaatgaaa tgtctgagtg agggccatga tcaaaaataa aatggaaacg atcaaaatcg
    20221 aatgggcgcc aaacagatga tgacagaaaa caaggacaat agctcctgcg ataaaggcaa
    20281 gaacgacact tagatagtgg aaaaatcgat caaaatctgc tttttcttta gaaatacaat
    20341 aatcatataa gttcgttgct acccctttta aatttccagt tgtaaataaa ttagtgtagg
    20401 cacgcccgtt gattttatca aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa
    20461 tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg
    20521 cttcataata tagaacaaac aagcgccaga aatgtttttc tttttttcta aaataatcaa
    20581 ttagatactt tgttagaaga atccctaaga aaaagaaaag ggtagacaat acttttttac
    20641 caacacttag ccattctccg tcaaatgctt ggatgatcga tagaatgata ttgccagttt
    20701 gagcagaggc aaaagcacca tattggatat aagtataact gtccatggct cctccgataa
    20761 atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga agtctgcatt tttttcatca
    20821 ccttttatga atatttttgt cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa
    20881 ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg tctgttagta
    20941 taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca aattcaaaga
    21001 agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga ttacaaaaag
    21061 aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg ctacaagaag
    21121 aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt gaggaagaag
    21181 tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag aaacgttcct
    21241 ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag aaacatcttc
    21301 gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc
    21361 tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt acaaggaacg
    21421 ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc gctgtccata
    21481 tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga aaatttcctg
    21541 aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa aaagtgaatg
    21601 ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa tatggagaaa
    21661 aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta gaagaaatgg
    21721 ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag ccgatatcag
    21781 aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag caattgctag
    21841 agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag atccagcatt
    21901 atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact aatccacttc
    21961 aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt gttgttggag
    22021 atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc aagaactttg
    22081 aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct cgggcagcta
    22141 ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata ccaaaggcga
    22201 tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct gatactaaag
    22261 cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat gggaaatata
    22321 aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc caatctctgc
    22381 tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt atctttggta
    22441 tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc
    22501 aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac tgctacctga
    22561 aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc gttgcagatt
    22621 atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga gagtttgagg
    22681 aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa agctatcttc
    22741 atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata cgagaagaag
    22801 taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc caagcgacag
    22861 ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat gcactatgta
    22921 tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg attggttgta
    22981 acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac tttaatgaaa
    23041 taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat gttgcgatga
    23101 ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca agaaaattga
    23161 aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga
    23221 gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga aaattgcttc
    23281 tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa cacagtttat
    23341 tcggaaaagg gaggtgtgac aagaattttg tcacacctcc cttatttatt ttgaaaatga
    23401 gaatactatt atcaaaatag agaatatata gaaaaattct cattattcaa acgtaaataa
    23461 tcagttattt tgattttttt tatattttat tagctaataa ttggtatttt aaaagaaatt
    23521 actgtagaat tggattataa ttaaaaagga gtgagaaaat gataaaagta tatacttcgc
    23581 ctagctgtac ttcctgtaga aaagcaagag catggttgca agcgaatcaa cttgaatttg
    23641 aagagaagaa tatttttgcc gaaccgttaa cagaaagtga gatcaaaaag atcttggcag
    23701 cgactgaagg aggagttcaa gatattattt ccactcggtc gaaaatttac gaaaagctgg
    23761 gcatagattt taacgaatta actttaaagc aaatgatcgt cttattcaaa gaatatcctt
    23821 cattattgag acgacctatt ttgattgatg aaaaaagaat actaatcgga tttaatgaag
    23881 atgagatccg gtcatttgtc cctcgtgaaa ttcgatgtgt tgccagaaat ctgatctatt
    23941 tacaaaacaa taacatctta aattcatagg gagggagcct tcgtggaagt gaaagtctat
    24001 ggatcagcgc actgtgtaac aagtctgaag gcgaaacaat ggttagaaca ccatcaattg
    24061 ccttatcaat ttgttgactt agaagaacgc gatctaagta cagaagaagc agaagaatta
    24121 tgcagttttg aagaagtgca agcagaacag ctatttgcga cttggtctga ggcttttcac
    24181 aacattgcga ttgacctttc ctgtgtagga aaaaaacaac tgattttttt atgcacacaa
    24241 cataccaatc tcctcagacg tcctttgatt attatcgatg aagttctatt tattggatac
    24301 aatgagcagt tgatggaaca gcttatctta aataaataga ctttaactgt tttaatcaag
    24361 tcactgagca tttcatacag ttgtaacagt cgaacactta gcatcaagcg tgttatgtgt
    24421 tctttttttg actttatttt tctactatag agacatgctc aatttatgtg gtcaaaattc
    24481 aaatctaggt ggggatatag agttaaaaat atattgacat gtgtaaattt acatgatata
    24541 ttcatttctg ttgttttaag tagcaaagca ataaaatagt attttaaaaa acatataaaa
    24601 tagttgttga catgttagaa atgacatgat aagataactg agttgttaaa aaagaaccac
    24661 ttgtgaagct tgattctatc gaaaaaaact tcaaaaaagt tcttgacaaa taacaaatga
    24721 taagttatga taaatgagtt gctgtaagat ttcgaaaaac gaaatcaaaa aaacttttaa
    24781 aaaagagttg acaattactt gtctctctga tatgatataa aagttgctac ggcagccaaa
    24841 aacgaatcgg ggaacactcc tgatgaagta gacctttgaa aactgaacaa agtaagacaa
    24901 accaaatgtg tagggcgtct tgattcaatt ca
//
LOCUS       DACCSK010000034        24371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCSK010000034 DACCSK010000000
VERSION     DACCSK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.034"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IVP39_001191"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IVP39_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7586272.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IVP39_001192"
     CDS             complement(1551..2972)
                     /locus_tag="IVP39_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7586273.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IVP39_001193"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IVP39_001193"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7586274.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IVP39_001194"
     CDS             complement(5383..6639)
                     /locus_tag="IVP39_001194"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7586275.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IVP39_001195"
     CDS             7219..8520
                     /locus_tag="IVP39_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7586276.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IVP39_001196"
     CDS             complement(8589..9188)
                     /locus_tag="IVP39_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7586277.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="IVP39_001197"
     CDS             9442..10323
                     /locus_tag="IVP39_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586278.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11946
                     /locus_tag="IVP39_001198"
     CDS             10336..11946
                     /locus_tag="IVP39_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7586279.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12023..12682)
                     /locus_tag="IVP39_001199"
     CDS             complement(12023..12682)
                     /locus_tag="IVP39_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7586280.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12965..13630)
                     /locus_tag="IVP39_001200"
     CDS             complement(12965..13630)
                     /locus_tag="IVP39_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586281.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IVP39_001201"
     CDS             complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IVP39_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7586282.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IVP39_001202"
     CDS             complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IVP39_001202"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7586283.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IVP39_001203"
     CDS             complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IVP39_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7586284.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15944..16264
                     /locus_tag="IVP39_001204"
     CDS             15944..16264
                     /locus_tag="IVP39_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7586285.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16376..17245)
                     /locus_tag="IVP39_001205"
     CDS             complement(16376..17245)
                     /locus_tag="IVP39_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7586286.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17341..18645)
                     /locus_tag="IVP39_001206"
     CDS             complement(17341..18645)
                     /locus_tag="IVP39_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7586287.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="IVP39_001207"
     CDS             complement(18642..19151)
                     /locus_tag="IVP39_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586288.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21460)
                     /locus_tag="IVP39_001208"
                     /pseudo
     CDS             complement(19326..21460)
                     /locus_tag="IVP39_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(21543..23198)
                     /locus_tag="IVP39_001209"
     CDS             complement(21543..23198)
                     /locus_tag="IVP39_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7586289.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23200..23514)
                     /locus_tag="IVP39_001210"
     CDS             complement(23200..23514)
                     /locus_tag="IVP39_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7586290.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23684..23944
                     /locus_tag="IVP39_001211"
     CDS             23684..23944
                     /locus_tag="IVP39_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7586291.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23958..24272
                     /locus_tag="IVP39_001212"
     CDS             23958..24272
                     /locus_tag="IVP39_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7586292.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6945 a         5036 c         4397 g         7993 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcata tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat gatttttttc
    10801 caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt agtcttgatt
    11401 tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac
    11461 ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat
    11521 atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg
    11581 ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa
    11641 gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc
    11701 ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc ctttgcactc
    11761 aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc
    11821 tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt
    11881 atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa
    11941 ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa
    12001 catttggagg tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga
    12061 cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata
    12121 aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa
    12181 agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta
    12241 ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga
    12301 ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt
    12361 gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa
    12421 cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt
    12481 cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt
    12541 gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa
    12601 gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa
    12661 cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt ctaataaaga
    12721 aacataactt cccttattcc aactgataat tttcttttat catagcataa acgtcaagcg
    12781 aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt ttccttcttt
    12841 tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt gaataatcaa
    12901 aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg
    12961 ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg
    13021 tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca
    13081 tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca
    13141 aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat
    13201 aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg
    13261 ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt
    13321 attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc
    13381 ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag
    13441 gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc
    13501 atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc
    13561 tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc
    13621 caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata
    13681 tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa
    13741 aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa
    13801 aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg
    13861 ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc
    13921 ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca
    13981 aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt
    14041 tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt
    14101 cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt
    14161 cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc
    14221 ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc
    14281 gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa
    14341 ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct
    14401 tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt
    14461 ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt
    14521 accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt
    14581 catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc
    14641 ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac
    14701 tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata
    14761 ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc
    14821 tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa
    14881 ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt
    14941 acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc
    15001 tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa
    15061 gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc cactatcggc
    15121 ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac
    15181 cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc
    15241 tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga
    15301 ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac
    15361 aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat
    15421 tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg
    15481 caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt
    15541 ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa
    15601 aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc
    15661 cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct
    15721 gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac
    15781 tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca
    15841 ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt
    15901 cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac
    15961 gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat
    16021 tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg
    16081 ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc
    16141 cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt
    16201 attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag
    16261 ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa
    16321 gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt
    16381 tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat
    16441 aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg
    16501 gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg
    16561 ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt
    16621 caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg
    16681 ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta
    16741 attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt
    16801 ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt
    16861 tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt
    16921 ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat
    16981 cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt
    17041 ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag
    17101 caccagaaga gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa
    17161 aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca
    17221 ttcttttttt catcactttt cccatcctta ctacttttta actaatttct tattataagc
    17281 aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt
    17341 ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt
    17401 gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat
    17461 atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga
    17521 agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt
    17581 aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc
    17641 aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat
    17701 gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt
    17761 cagccagact tctgttccac cttttgccca acgttttctg tgtcgatata gcatcttcaa
    17821 cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt
    17881 taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc
    17941 cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc
    18001 accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt
    18061 ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc
    18121 aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc
    18181 cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat
    18241 attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta
    18301 ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac
    18361 taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatattcgat
    18421 cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg
    18481 aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat
    18541 aaaccataca aatccgccta aaatcggata agaaacgaga aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttg taaaagtttc cttgcataag
    20161 ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc aactgcctat
    20221 cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt ttcaaaagat
    20281 gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg atcaatttag
    20341 gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg actgtttttc
    20401 catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga tctgattgtt
    20461 ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat acattttctc
    20521 ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa agctgtgaaa
    20581 ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt aggacattcg
    20641 aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg gatgtcatcg
    20701 tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag gttttaccac
    20761 ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg attgtgtttt
    20821 gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct tggcctaaga
    20881 cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg accaaacgat
    20941 ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta ttttgacgaa
    21001 ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc
    21061 aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg
    21121 attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag ggacttttca
    21181 ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata ttcatctgat
    21241 cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct aagtaactgt
    21301 caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct tctatttttg
    21361 ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga tacacgttct
    21421 tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc ctcttttttt
    21481 aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct ttttaacgag
    21541 tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa agaaaatgca
    21601 ccatcaaggt tttttacgtg gtagccagac tgcttcaaga ttctttccgc tagatagcta
    21661 cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc atttaatcgg
    21721 tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt ttccaattct
    21781 tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg caattcatgc
    21841 cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc tagcatgttt
    21901 actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc cggtaaatcg
    21961 aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt atccacacct
    22021 tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa gatcaacttc
    22081 agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga tacatgaatg
    22141 acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt ggatgcggca
    22201 gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct tcgagaaatt
    22261 ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc aagagaaata
    22321 agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac cgcataaata
    22381 tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat gcctaattcg
    22441 attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa aataactaaa
    22501 tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca
    22561 gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt ttctataaaa
    22621 gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat caccatgact
    22681 tctaaacctc ttctttttag gttttctgcc atttccaatc cgatgaatcc tgcaccaacg
    22741 atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat atctggtaca
    22801 tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg aacaactggt
    22861 tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg accctcatgt
    22921 acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt tggacgcaca
    22981 tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt ttcccgttca
    23041 ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac atacggtcct
    23101 ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt agcagcagac
    23161 attccacctg ctacacctcc gacaataacg attttcatct tatttccctc ctactgttcg
    23221 atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag atttctttaa
    23281 ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga cggtcttttc
    23341 tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc cttttatatg
    23401 cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt ccaaaagctt
    23461 ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat tcattttctg
    23521 cacctcattt acatttttat aaatacctca tggggtatat taaacaggag ctttattaat
    23581 atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca taaaataccg
    23641 ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga ttgataataa
    23701 aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat
    23761 cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc gttctagtat
    23821 tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg agaaacctga
    23881 tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga ttattaaaaa
    23941 gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa aaatggtcag
    24001 aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg attgtcgtat
    24061 ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa ttcttagatt
    24121 tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat atttcagaaa
    24181 gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta gttgaactgc
    24241 aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca gaagtgatca
    24301 gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa
    24361 ggttgtgaaa a
//
LOCUS       DACCSK010000035        24201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCSK010000035 DACCSK010000000
VERSION     DACCSK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.035"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            369..998
                     /locus_tag="IVP39_001213"
     CDS             369..998
                     /locus_tag="IVP39_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586293.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1122..2231)
                     /gene="ugpC"
                     /locus_tag="IVP39_001214"
     CDS             complement(1122..2231)
                     /gene="ugpC"
                     /locus_tag="IVP39_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7586294.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2440..2862
                     /gene="mgsA"
                     /locus_tag="IVP39_001215"
     CDS             2440..2862
                     /gene="mgsA"
                     /locus_tag="IVP39_001215"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7586295.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            3185..3721
                     /locus_tag="IVP39_001216"
     CDS             3185..3721
                     /locus_tag="IVP39_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7586296.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3761..5524)
                     /locus_tag="IVP39_001217"
     CDS             complement(3761..5524)
                     /locus_tag="IVP39_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586297.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKNIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5527..7242)
                     /locus_tag="IVP39_001218"
     CDS             complement(5527..7242)
                     /locus_tag="IVP39_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586298.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSIPEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(8021..8245)
                     /locus_tag="IVP39_001219"
     CDS             complement(8021..8245)
                     /locus_tag="IVP39_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586299.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            8445..11213
                     /locus_tag="IVP39_001220"
     CDS             8445..11213
                     /locus_tag="IVP39_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7586300.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            11294..11713
                     /locus_tag="IVP39_001221"
     CDS             11294..11713
                     /locus_tag="IVP39_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7586301.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            11725..12195
                     /locus_tag="IVP39_001222"
     CDS             11725..12195
                     /locus_tag="IVP39_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7586302.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            12212..12991
                     /locus_tag="IVP39_001223"
     CDS             12212..12991
                     /locus_tag="IVP39_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7586303.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            12978..13799
                     /locus_tag="IVP39_001224"
     CDS             12978..13799
                     /locus_tag="IVP39_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7586304.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            13816..14865
                     /locus_tag="IVP39_001225"
     CDS             13816..14865
                     /locus_tag="IVP39_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7586305.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            14878..15882
                     /locus_tag="IVP39_001226"
     CDS             14878..15882
                     /locus_tag="IVP39_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7586306.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            16515..17534
                     /locus_tag="IVP39_001227"
     CDS             16515..17534
                     /locus_tag="IVP39_001227"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7586307.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            17577..18458
                     /gene="galU"
                     /locus_tag="IVP39_001228"
     CDS             17577..18458
                     /gene="galU"
                     /locus_tag="IVP39_001228"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7586308.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            18758..19135
                     /locus_tag="IVP39_001229"
     CDS             18758..19135
                     /locus_tag="IVP39_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586309.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            19741..20355
                     /locus_tag="IVP39_001230"
     CDS             19741..20355
                     /locus_tag="IVP39_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7586310.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            21128..22867
                     /gene="yfcC"
                     /locus_tag="IVP39_001231"
     CDS             21128..22867
                     /gene="yfcC"
                     /locus_tag="IVP39_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7586311.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            23024..>24201
                     /gene="iadA"
                     /locus_tag="IVP39_001232"
     CDS             23024..>24201
                     /gene="iadA"
                     /locus_tag="IVP39_001232"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7586312.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     "
BASE COUNT         7803 a         4132 c         5014 g         7252 t
ORIGIN      
        1 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
       61 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
      121 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
      181 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
      241 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
      301 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
      361 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
      421 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
      481 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
      541 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
      601 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
      661 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
      721 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
      781 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
      841 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
      901 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
      961 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
     1021 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
     1081 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
     1141 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
     1201 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
     1261 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
     1321 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
     1381 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
     1441 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
     1501 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
     1561 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
     1621 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
     1681 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
     1741 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
     1801 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
     1861 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
     1921 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
     1981 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
     2041 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
     2101 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
     2161 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
     2221 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
     2281 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
     2341 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
     2401 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
     2461 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
     2521 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
     2581 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
     2641 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
     2701 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
     2761 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
     2821 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
     2881 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
     2941 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
     3001 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
     3061 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
     3121 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
     3181 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
     3241 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
     3301 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
     3361 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
     3421 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
     3481 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
     3541 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
     3601 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
     3661 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
     3721 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
     3781 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
     3841 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
     3901 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
     3961 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
     4021 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
     4081 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
     4141 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
     4201 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
     4261 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
     4321 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
     4381 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
     4441 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
     4501 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
     4561 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
     4621 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
     4681 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
     4741 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
     4801 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
     4861 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
     4921 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
     4981 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
     5041 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
     5101 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
     5161 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
     5221 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
     5281 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
     5341 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
     5401 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgttttttg
     5461 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
     5521 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
     5581 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
     5641 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
     5701 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
     5761 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
     5821 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
     5881 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
     5941 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
     6001 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
     6061 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
     6121 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
     6181 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
     6241 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caggaatcga
     6301 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
     6361 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
     6421 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
     6481 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
     6541 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
     6601 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
     6661 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
     6721 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
     6781 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
     6841 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
     6901 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
     6961 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
     7021 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
     7081 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
     7141 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
     7201 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
     7261 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
     7321 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
     7381 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
     7441 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
     7501 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
     7561 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
     7621 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
     7681 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
     7741 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
     7801 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
     7861 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
     7921 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
     7981 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
     8041 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
     8101 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
     8161 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
     8221 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
     8281 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
     8341 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
     8401 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
     8461 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
     8521 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
     8581 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
     8641 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
     8701 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
     8761 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
     8821 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
     8881 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
     8941 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
     9001 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
     9061 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
     9121 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
     9181 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
     9241 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
     9301 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
     9361 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
     9421 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
     9481 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
     9541 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
     9601 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
     9661 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
     9721 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
     9781 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
     9841 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
     9901 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
     9961 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    10021 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    10081 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    10141 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    10201 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    10261 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    10321 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    10381 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    10441 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    10501 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    10561 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    10621 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    10681 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    10741 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    10801 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    10861 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    10921 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    10981 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    11041 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    11101 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    11161 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    11221 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    11281 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    11341 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    11401 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    11461 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    11521 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    11581 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    11641 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    11701 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    11761 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    11821 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    11881 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    11941 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    12001 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    12061 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    12121 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    12181 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    12241 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    12301 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    12361 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    12421 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    12481 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    12541 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    12601 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    12661 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    12721 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    12781 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    12841 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    12901 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    12961 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    13021 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    13081 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    13141 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    13201 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    13261 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    13321 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    13381 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    13441 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    13501 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    13561 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    13621 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    13681 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    13741 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    13801 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    13861 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    13921 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    13981 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    14041 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    14101 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    14161 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    14221 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    14281 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    14341 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    14401 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    14461 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    14521 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    14581 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    14641 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    14701 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    14761 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    14821 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    14881 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    14941 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    15001 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    15061 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    15121 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    15181 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    15241 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    15301 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    15361 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    15421 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    15481 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    15541 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    15601 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    15661 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    15721 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    15781 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    15841 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    15901 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    15961 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    16021 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    16081 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    16141 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    16201 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    16261 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    16321 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    16381 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    16441 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    16501 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    16561 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    16621 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    16681 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    16741 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    16801 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    16861 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    16921 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    16981 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    17041 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    17101 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    17161 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    17221 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    17281 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    17341 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    17401 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    17461 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    17521 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    17581 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    17641 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    17701 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    17761 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    17821 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    17881 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    17941 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    18001 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    18061 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    18121 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    18181 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    18241 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    18301 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    18361 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    18421 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    18481 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    18541 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    18601 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    18661 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    18721 cgaatttttt aattataaaa aaaggtgtgt gttcactatg ggaaaattag tcgctgttgc
    18781 acagtcgctg cttgatatac tcaagtggct gatggaagga agcaattgga tatttgtgtt
    18841 gccactattg cttattagtc tatttatcat gtttcgcatg gtcagaaaaa aagaaacgtt
    18901 tctgattttt atctggtatg cagcatccgt tggggttgct accttgatgg ttgaattggc
    18961 gttaggtagt tgggaagctt gcgtatttgc tttatttttt gctgtactgt atctaatcat
    19021 ttatatccca tatatgaaaa gaaagtctcg gataggcaga aaaggtagac atacaactac
    19081 tttttctagt acagattctc tcgaacaaag tagaagaacg agaagaagac ggtaatattt
    19141 agtaggtata cgcaaaaaaa gataaaataa tggataaact attgagatga gagatttatt
    19201 taattggtgg aaaaaagctt ttttcactta tataaaagaa tcttttttaa aggaatgctt
    19261 gttgtatcgg gataagccat tccttttttt atgttattta tatatatata aatcatacca
    19321 atgttttttt gtattttctt gcttgcactt ggataagaga ttacttacaa taaattcaga
    19381 tagaaacagc tgaataaaaa aaggctccct gacccttagt tggcgctaag aatcagggaa
    19441 tgaccagtca cacacataca ccctgaccga tcattttgcc atagtcaatg cagtgcatcg
    19501 acctaattta ttgtacctaa tctagggtga aaattctacc cctatgtaga tattttccat
    19561 ggtctgcacg ttgttttatt tgctatgtca aggtattggg cgtattcgtt caatgccttt
    19621 tttgtgtttg aaacagcaaa gaccacatgt gttactgtct aactactgtt tttttatgta
    19681 cttccttaga ttaggcagct gtttctattc atgtcaagta tagctaggcg ggagagataa
    19741 atgagtttat gttattttga ctattatcac caattggaag agaaattaag gatagaagag
    19801 ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa ttaatcaagg tggtagattg
    19861 cagatatatg ctagccgcac actaagtggt gtagcatttg aattttggga tcaaaccccg
    19921 gatcttgtac caagtatatt gattgaaaat ccagccggag gtgtctatga aacgctagaa
    19981 ggtgcaggac aggcaattat tgatcagctt tctgtcaaat cagaagacat ttttcttctt
    20041 ctttcgaatg aaggaagaaa cccggcaatc atcgaactag ctcagtggat caaagaaaat
    20101 ggtcatccat taatcattgt tactggattt gatttgtcac gaagtatcaa atcgcaacat
    20161 agcagtggtt tgcgattatt tgaatttgct gatttggttt tggacaacca cgccaattta
    20221 catgatgcag tcttgacttt gccaaatcta gatccagcga tctgcggaac agcctctctt
    20281 gcaacgatct ttttgctgca gcaagttttg tattttacag caaaacagct agtccaggac
    20341 acaagtggaa aatagttatc cacatgttga caaagttcct taaaaaaata atgttccaca
    20401 taaaaatgga gcgtttgaaa aaaagaaaac gattattaat gaggattaac aacgactact
    20461 ttttttgttt tagttatttt aagatggatc atctttgatt ttaagcttgt tttgtttaaa
    20521 gtttgttatt tttataagtt gtttcacagt tatgagaaat gagttataag aatatgtagt
    20581 gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag aatccatatg gattctttct
    20641 tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa ctctttacaa agtaattata
    20701 gtacatttgt tatcattatt gcaatgaagt ttacctgata taacaaaatt gtccaacagg
    20761 ataccttttt ctaatagaaa taaaatacac aaaaattgaa ggatagataa tggatattta
    20821 taagtgttta ttattttttt tgcaatgaaa ttcaattttt caatgattgt aaaatgaaaa
    20881 aaacaggacg agattttctc taatctaaaa ataaaaaaag aattttaagg aaaaacagag
    20941 aaataaaata cttaatataa atggaaagag aaaaaaataa tcatacatag gagaaaaaaa
    21001 acaaaataga aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg
    21061 tataattcat ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga
    21121 aataaatttg aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc
    21181 gacaccccat acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt
    21241 tgtcgttcca gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga
    21301 aacaagtact cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa
    21361 gtcgtatgtg tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact
    21421 ggatgttccg gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca
    21481 ggaaaaacta gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa
    21541 tatctatgat acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt
    21601 tggcggcttt ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg
    21661 atctgctgta gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat
    21721 gcgtaccgga gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga
    21781 gcgtttagct ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat
    21841 ggcagaagag gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata
    21901 tgactcgatc gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc
    21961 ttggatgagc ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc
    22021 tggtgcgaca ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat
    22081 gatgatatat gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga
    22141 tgattatttc cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg
    22201 gcataaattg atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac
    22261 tcaaaaaggc tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc
    22321 tggaatcatt gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc
    22381 tttccaatct ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg
    22441 gattcttctt gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt
    22501 gcatgggatc gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta
    22561 catcttccaa agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac
    22621 tatgccaatc atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct
    22681 tgcttatcag cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat
    22741 tggtgtgcta ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat
    22801 gcaaggattc ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct
    22861 gcagtagtac tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa
    22921 gaaccgtcat tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt
    22981 aagatcctat catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca
    23041 aattgaagtg tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa
    23101 gattttggct gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact
    23161 ttcaggtgag ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg
    23221 tggaggagga gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac
    23281 tacagctggt gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat
    23341 gactgcgttg atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata
    23401 cgaaggttcg taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt
    23461 gacaattgat aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca
    23521 gccaagtttt gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc
    23581 cggaaaagct gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt
    23641 gacacgaatc gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa
    23701 tcgcacacca gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga
    23761 cttcactgct agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag
    23821 caaagcactg aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc
    23881 agatggacaa ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt
    23941 aggaagtgcc aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc
    24001 tttggaaatc gccttacgag ctataaccag taatccagca cgtatattga aattggataa
    24061 aaaaggaaaa atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct
    24121 tgatattgat acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt
    24181 atgggggact ttcgaaaaaa g
//
LOCUS       DACCSK010000036        23782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCSK010000036 DACCSK010000000
VERSION     DACCSK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.036"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            782..1822
                     /locus_tag="IVP39_001233"
     CDS             782..1822
                     /locus_tag="IVP39_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7586313.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2647..2979
                     /locus_tag="IVP39_001234"
     CDS             2647..2979
                     /locus_tag="IVP39_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7586314.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3598..3753
                     /locus_tag="IVP39_001235"
     CDS             3598..3753
                     /locus_tag="IVP39_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7586315.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYD"
     gene            3757..3939
                     /locus_tag="IVP39_001236"
     CDS             3757..3939
                     /locus_tag="IVP39_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7586316.1"
                     /translation="MTDNPIGFGLLPEDDEGDEWFKMTLTNDKGDELSVEDTWSYLSD
                     YIVSVEIIDFVADKEE"
     gene            4106..4345
                     /locus_tag="IVP39_001237"
     CDS             4106..4345
                     /locus_tag="IVP39_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586317.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARSYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRIYNGIKDLKPARPVVKRKKKITQE"
     gene            4455..4649
                     /locus_tag="IVP39_001238"
     CDS             4455..4649
                     /locus_tag="IVP39_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586318.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYISDMQITGKEKACLLGSMKNFE
                     QLRRTYVKIRSNCQLLLEVS"
     gene            4776..5465
                     /locus_tag="IVP39_001239"
     CDS             4776..5465
                     /locus_tag="IVP39_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP7586319.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGHQKDKIAEKVDGVTK
                     TIHIQEQDKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            5471..6637
                     /locus_tag="IVP39_001240"
     CDS             5471..6637
                     /locus_tag="IVP39_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7586320.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            6719..7390
                     /locus_tag="IVP39_001241"
     CDS             6719..7390
                     /locus_tag="IVP39_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7586321.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            7443..9419
                     /locus_tag="IVP39_001242"
     CDS             7443..9419
                     /locus_tag="IVP39_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7586322.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            9432..10184
                     /locus_tag="IVP39_001243"
     CDS             9432..10184
                     /locus_tag="IVP39_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7586323.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            10200..10961
                     /locus_tag="IVP39_001244"
     CDS             10200..10961
                     /locus_tag="IVP39_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586324.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            10974..11234
                     /locus_tag="IVP39_001245"
     CDS             10974..11234
                     /locus_tag="IVP39_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586325.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            11231..13321
                     /locus_tag="IVP39_001246"
     CDS             11231..13321
                     /locus_tag="IVP39_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7586326.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAVSDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(13357..15414)
                     /locus_tag="IVP39_001247"
     CDS             complement(13357..15414)
                     /locus_tag="IVP39_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7586327.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(15559..15750)
                     /locus_tag="IVP39_001248"
     CDS             complement(15559..15750)
                     /locus_tag="IVP39_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586328.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            15938..16393
                     /locus_tag="IVP39_001249"
     CDS             15938..16393
                     /locus_tag="IVP39_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586329.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            16390..16632
                     /locus_tag="IVP39_001250"
     CDS             16390..16632
                     /locus_tag="IVP39_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586330.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            16650..16952
                     /locus_tag="IVP39_001251"
     CDS             16650..16952
                     /locus_tag="IVP39_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586331.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            17021..19075
                     /locus_tag="IVP39_001252"
     CDS             17021..19075
                     /locus_tag="IVP39_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586332.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            19075..21687
                     /locus_tag="IVP39_001253"
     CDS             19075..21687
                     /locus_tag="IVP39_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7586333.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            21684..>23782
                     /locus_tag="IVP39_001254"
     CDS             21684..>23782
                     /locus_tag="IVP39_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586334.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQS"
BASE COUNT         8461 a         3621 c         4693 g         7007 t
ORIGIN      
        1 aagtatatat atttaaaaat ttaagattaa tttttttatg acttatacaa atttatctga
       61 tattctctta ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt
      121 atttaattgc aatttggtag tattttttta ttatcttatt cctagaacgg tagtacttaa
      181 tataaatata ggtagtatct aatttaagta ttaatggtag tatctaattt aacaggtagt
      241 atgtaactac tttaaagtaa ttattttcag attaacacag tagtatctta ttaaataagt
      301 taattttaat ggtagtacat aattattata tatatctgac attggtagta cttattgtat
      361 tttttagtag tatataatct attgttttct agcctaaagc cttgtcttat aagaataata
      421 taatatatac tactgaaata aggtagtata tatcactagt aatggtagta tgttttatat
      481 gcataaggta gttcttaatt tattttttgt tttgacacta gtagtactta attatcttta
      541 attttaaaac taaattttat tcatacgttc ttctggtagt atttaattat aaataatatt
      601 tatttaaagt agttcttaat gactttaaag gtagtacata attcatttta tttctgattt
      661 taggtagtat ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct
      721 attttcgaaa agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac
      781 aatgactgat tttaattttt ttaaggcgga tagagtttat aacgaattat tttatcaatt
      841 tccaaaagtc tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc
      901 ctatatgctt ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga
      961 aggtaatgtt tattttactt atacgacaaa tgaactatgt agcgtattaa actgccaaaa
     1021 acaaaaagcg atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca
     1081 gatgggattt aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt
     1141 aaaagtctca gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa
     1201 cgttgataaa tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga
     1261 atcccttggg gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga
     1321 gggtatgaaa atcataccag aacttaataa taatatatta gacactaata gacacaatat
     1381 agacactgaa aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat
     1441 gacaaacgac agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt
     1501 ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa
     1561 agctgaaata gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa
     1621 tgcagaacaa gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa
     1681 agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc
     1741 aatagctgct aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat
     1801 tacgaaagac tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa
     1861 cattaaaatt aaaaattttt tattattcta attatattac gaatattaca aaataatagc
     1921 tattttaata gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta
     1981 cgtcataagt gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc
     2041 atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca
     2101 agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt
     2161 tttgtataaa tttaattgtc tgctatgcct gtgatgaatt gattttcgtt ctgcactttt
     2221 ggacgataaa tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag
     2281 gctggtaatc ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac
     2341 ctttcagtgt tacggtatta cccgcaacgg atttagtata acagatattc aaaagtgata
     2401 ccactttttt tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta
     2461 agcataatgt tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag
     2521 agggaacgca tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc
     2581 tctatttttg aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt
     2641 tattttatgt catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg
     2701 tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg
     2761 cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt
     2821 gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca
     2881 atcaaacatg tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg
     2941 cgttgtaccg caaaagggat ggggcgtttg atcagctgaa ttgatcattc tcataacttg
     3001 tattttatcg tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt
     3061 cactagaaaa ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg
     3121 taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga
     3181 tagtggtgaa atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa
     3241 gttagacaag ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact
     3301 ttaatgcaaa ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa
     3361 attgtgtcgt gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat
     3421 cgagcattta ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat
     3481 tttttatgta tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt
     3541 gaaaaaattg atataaaaca tagagaaaat gaaatttacg taatggagga atgacaaatg
     3601 aaaaattatg atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt
     3661 tgggattaca aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt
     3721 ttagcattag acgaggatga tttatacgat taaacattga ctgataatcc aattggattc
     3781 ggcttgctac ctgaagatga tgaaggcgat gagtggttta aaatgacgct tacgaacgac
     3841 aaaggagacg aattgtctgt agaagatacc tggtcatatt tgagtgatta tattgtcagt
     3901 gttgaaatca ttgactttgt agcggataaa gaagaataaa aattggagga atagatcatg
     3961 aaatttttcg aagtaaaatc accatgctat tatgcgttaa tcgcagaaaa taatgaagaa
     4021 gaatgtgcag aaattacgac gaataataaa aagctagtat tatcattaga catcgagtaa
     4081 agaatttatt taggaggcaa attagatggc gcgaccagtt ttagatgcga tggatatgga
     4141 tactttgaca aaaggaaggt atgtacagct gttagctcgt agttattcac ctaaagatgt
     4201 atttaaagcc ttatccaaag gaacagatat ggaaaaagaa agattacgaa aagagtttga
     4261 ttattggaga atctataatg gaatcaagga tttgaaacca gcaaggccag tagttaaaag
     4321 aaagaaaaag ataacccaag agtgaagtgt agagcgtatg tgatcatagg cttttttatt
     4381 tttatcaaaa caaggaaggt aggaaaactc atgaattgta aagaaatcga aaatagaaaa
     4441 aagtgtcgaa agaaatggaa gaaaagttac taaaaacgat gaaacaaaaa caccttaaaa
     4501 ggttatctgt catgcaatat atcagtgaca tgcagataac aggaaaggag aaagcctgtc
     4561 tattgggatc aatgaaaaat tttgaacagt tgagacgaac ttatgtgaaa ataagatcta
     4621 attgccaact attattagaa gtttcttgac gattatgata tgaaaatgat caattatgat
     4681 gtggcattgt tatttaataa aaagaatagt aaaatttaac gcggtttatc ttggttagat
     4741 gtaggtaaat taattcaaat atagaaggta gatagatgaa taaaaacaat aaaactttat
     4801 ttaatcctta cgtgggcgta atcattgttt ttctcctttt tagttatttt gcttatcaag
     4861 gtcatcaaaa ggacaaaatc gctgaaaaag tagatggtgt aacaaaaaca atccatattc
     4921 aggaacagga taaaacctat caagataatt taaccagttt aacggatatt tctaagagac
     4981 aacttattac taatcaaaaa caggcattag aaaaggatct caaaggtgaa attgctggaa
     5041 aacttgtttt ttcttctaca ggatttattc tccctgtttt tcaaggagcg aatagcgata
     5101 cgcttagttt aggagtagct tctacttatt atccagatgc tgaaatggga aatggaaatt
     5161 atattcttgc tggttataac gtagatacac caagcgtttt attatccgat atgattgatt
     5221 tatctgaagg agaagaaatt gatctaatcg atgctcatta caagtatcag tatgaagtct
     5281 ccaaaagagg aaaaatttat gattacagcc aggtagttca atctaaggaa aacaattcct
     5341 ttttatcctt gccaaaagca ggtaaaaaac cactactcac actgttagcc tttcaagcaa
     5401 ataatatgaa aaagtgcgat gtgatccagt gtgaattagt caatgtagaa aaaatcaata
     5461 aataagggaa gtgagtcaca ttcaagaact tgtagaaaaa ttgatcaata aaatgagtgc
     5521 catttttctg ttactgttgg tatccattct atatttaata tggacggtgg ggttaaatcc
     5581 tcctacgata ttatttattt atgataatgc tgcacagtta gtgacgtttt tcgtaggctg
     5641 tttgtttctt ttgaatctgt acaaggtaaa gagaaacgat ctattcttca ttgcagcagg
     5701 cattcttttt taccttttct tttctttttt tagaagctac cgaggagata ctttctatgg
     5761 tgatctgatc gttccgacaa ttattgctat tattttgatt gtaaaatgga cgaaatttac
     5821 tgtaattgat cgtagcttgt atcttgtagt attctatgtc tttttactcg taacggttta
     5881 tcgagtattt actgagatac cagtaccaga aggacaaagt atttggaatc catcgaacaa
     5941 attatcagat atttggatca atacaaatac gataggtagt tctttaatga cattagccct
     6001 tcttatcaca ggatttgcta gttcatttgg aaaatggtac atgcgattgc tatcattacc
     6061 agcgttgatt cttgctgggg caacgatttg ggtttgccaa tcgaaagcag cattatatgc
     6121 gcttgttatc ttcattatct tagaaatttt acctaagaag gtttttcgaa atacacactt
     6181 gtccttttgg atgtatgcag cagtttcggt tctagcttta ccgatttctt attttgctgc
     6241 aatgtcagac aaagttcact tatttacagg acgagaagaa atttggcgca aattctatga
     6301 aacattaggc gaaaaaacag gacaaatatt tgtagggatg aaacctttct ttttccatag
     6361 aggaaatcag attctaggta atcataatac gtatcacgca ctcgtcaatc actatggatt
     6421 gattggtttt gttctatcga ttattctttt gcttagtttc ttatggttgc tcgtaagaaa
     6481 acgtgagctg acaaatggac aactaacatt cttgtgggct tttttagcga tcatgtgtca
     6541 atcgttcatg gaagaaacat taacgacaat cagttgggtg ccaattgtat tcttaatgtt
     6601 agcaatggct acacaccaat ataaaaccac cagataaatg aaatagaata gtaataagag
     6661 aaaacacttt ttcgttgtga aaaagtgttt tttgttttgt ctgaaaggag gcagatggat
     6721 gaaaaaatgg ttatttggtt ttttaggagt cgctttaatc gtcgtttgca gtgtgtttgg
     6781 ctatgttagc taccaaaaac atgaaggaga ggtgtttaag caaaatatag aaaaaaagat
     6841 gcccgtagat caaatcaatg cacacgcaaa gagttataag gaggatgcaa cgaacgtcaa
     6901 taatgatatg tctttggggc aaatgttgtc catacaaaaa gaagcaatca aaatgggcgt
     6961 caataaacaa gtatttgcac aaattcagat tcctgcactt ggtttagcat tgccaatttt
     7021 taaaggggca aatcaataca ctttgagtct tggagcggct acttattttt atgaagatgc
     7081 caaaatgggc aaaggaaact atgtcctagc aggtcataac atggaaatgc caggtgtttt
     7141 attttctgat attcaaaagt tgtcattagg ggaggtgatg gacttagtta gtaacgatgg
     7201 cgtctatcgt tacaaggtaa cgcgtaaatt tatcgtacct gagtacttta aattaatcga
     7261 tggcgttcct gaagagaact ctttcttgtc tttaccaaag aaaggagaga aacccttatt
     7321 aacgttgttt acttgtgttt atacgtcaca aggaaaagaa agatacgtgg tacagggaga
     7381 gctacaataa caagaattta atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa
     7441 aaatggcaat aaatcggtat gtaaaaaaag ctggaatgat tgttggaatg ttaggaatca
     7501 ttgcaggctg ctttcagggg tatcgagctt atgcagaaga tgtcacgcaa aaaacgccac
     7561 cagaaagggt caatatcacg gttcataaat taatgtacga tcaaagtacg caattgaacg
     7621 ttgatattga cggaatcaaa aatgatggtt atacgcacga tcaatatcct acaggtgtaa
     7681 cgaaatacaa caaggcagat tatggtgatg ttgagtttac gttaggaaat attacggatc
     7741 aagtattacc gactgaagat tctgatctaa ccaacgcgaa agtggacgaa atcgttaaag
     7801 atgtagaaga caagggatct aacagtgagt acgttaagaa tgcaaccaat aaaattactt
     7861 cagctgtaga tgaaaatggt gagatcactt ttgctgatca accagcctat gtcaattcca
     7921 aagggaatgt atatgtggtt tatgaaagta aatccgctgc tggattagtc acacaaaaag
     7981 caaaaccaat ggttgtgatt gcgccaatga cagacaatac aggatcagga tttttaaaag
     8041 atattcattt gtatccaaag aacattgttt ctaagttgag ttttgaatta acgaaatttg
     8101 gtgacgatgg cacagctcaa agcaaacaaa cacctttaaa aggggcaaaa tttgagttat
     8161 acaagggcga acctggaaaa ggaacaaaat taggagagct tgtatcggat gatcaaggaa
     8221 agctgactgc aactgatttg acgttaggaa aatactactt tgtggaagtt ccttcagaag
     8281 ttgtagtggg atcagatcaa gaaccaacag cagaccaata tttgttaggc gcagacgctc
     8341 gcaacgatgc gcacaataag ttaacctttg aaattacgaa tgacggcgtg acaagtgatt
     8401 taaaagcttc ctacgtgaac tataaagcac cagtgatcga taaaacagtg acaaatggaa
     8461 caggagaaga acattctttc caaattggtg acgcagtgaa ttatcaaggt acaattcata
     8521 tccctacaga tatcgcaggt ggaacagatg ggattacagt aaatggtgta aaaagtgaaa
     8581 cttctcctta cagtgtattt aaatggggcg acacagcagg taaaggactt tcttatgttg
     8641 ctgaaaaagc agatattaaa gtaacaaata atgatggttc agtcgtatta aaagaaaata
     8701 ctgactataa aatcgaaaat tctgatagtg gttttgtgat cgattttatc gtaaataatg
     8761 gacaagtaag tgatacagtt gcgaatttac atggtcaaga tctaaaaatg acttataaca
     8821 tgtttgtgaa tgatagtgct gaaatcgcaa atccattaac aaatagcgtg gatttcgttt
     8881 ataacaataa tccatttaat caggaagaac accacgaaaa aacgaaagct gacgttgtga
     8941 cttatggcgc aaaattctta aaagtcgata gcggcctatt tggaacaggt atcaaagcaa
     9001 caccgcttga aggcgcagaa tttgcagcta agaatgcaga aggtaagtac tacggtggac
     9061 ttactgatac agataaagat ggtgtcaaag aagcagtttg ggtagacgat gtagcaaatg
     9121 cagcaatcct aaaatcagat aaagagggtc attttgaaat cactggttta actgaaggag
     9181 attactcact ggaagaaaca aaagctcctg aaaactatca aaaattaaca aaagagatct
     9241 ctttcaaagt agacaaagat tcatacaaag aagaaaaccg aatcacgatc aaaaataatc
     9301 aaaaagcttc cgtaccaatg actggtagca acggtttcca atcttacgta ttgatctctt
     9361 gcttgttgct tggggcaggc gcaatttctg cagttgttta ctttaagaag aaagcctaga
     9421 aggatcagct aatgattatc agacacccta agaaaaagcg aatcatgggt aaatggatca
     9481 ttgctttctg gttactttca gctgttggtg tcttactttt gatgcctgct gaagcgagcg
     9541 tggcgaaata ccaacaaaat cagcagattg ccgcaatcga tcgaacagga acggcagcag
     9601 aaacggattc ttctttggat gttgctaaga ttgagttagg cgatcctgtt ggtattctta
     9661 caattccaag tatttcttta aaactaccaa tttatgacgg aacttccgac aaaattttag
     9721 agaatggtgt agggattacg gaaggaactg gcgatattac aggaggaaac ggtaaaaatc
     9781 ctttaatcgc agggcacagt ggactttata aagataatct tttcgatgat ttgccttcag
     9841 ttaaaaaggg agaaaagttt tatatcaaag ttgatggtga gcagcacgct tatcaaatcg
     9901 atcggattga ggaagtgcaa aaagacgaac tacaaagaaa ctttgtcact tacttagaac
     9961 caaatcccaa tgaagatcga gtaacgctaa tgacatgtac gcctaagggg atcaacacac
    10021 accgtttcct agtgtatggg aagcgagtaa cctttacaaa atcagaatta aaagatgaag
    10081 aaaataagaa acaaaaatta tcttggaaat ggcttttagg aagtactgtt tttctttcag
    10141 tcatgatcat tggttcgcta tttgtttata aaaagaaaaa ataaaggagg gtaaccaaca
    10201 tgagaaatag aaaatggcta tttgtttgtg cagccgctct tttttctttc actgcctaca
    10261 cacagttgac gtttgcagac agacaagaaa gcacggatag cacagaacaa acaatccaaa
    10321 cgtcagatag cagcacgatt caaagcagcg atgaaaccgt cgattcgtca acagttgatt
    10381 cttcagaaga acctaaaatt gctgaagacg atcaaatagt caaacagacc attcacgttc
    10441 aaaaaattat ttcttccgaa caattagaca accaggggca aaagcttgaa aaacaagaag
    10501 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
    10561 ttaaggatct cactactgac caaattgaat cccaattaaa agatcgagtg aaaaaactat
    10621 ccagtgatca actgaagcta atttctaatg gagaaacaaa gacaattgat caacaaacag
    10681 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
    10741 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
    10801 ctgataaaaa tggtgaattc ttaaaggatg tgtggattta tcctaaatca gaagcttcgc
    10861 aacctaaaga agaagtaaaa aaaatcgtat ctactggcgt aaagaaaaac ttctttgaga
    10921 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
    10981 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
    11041 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
    11101 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
    11161 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
    11221 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
    11281 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
    11341 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
    11401 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
    11461 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
    11521 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
    11581 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
    11641 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
    11701 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
    11761 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
    11821 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
    11881 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
    11941 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
    12001 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
    12061 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
    12121 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
    12181 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
    12241 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
    12301 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
    12361 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
    12421 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
    12481 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
    12541 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
    12601 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
    12661 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
    12721 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
    12781 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
    12841 acaaaaatgg cacacaaaag gcgtagacgc tgtgtcagat cactacaaac aatttagtat
    12901 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
    12961 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
    13021 gctgaagtgg gaagcttccg ctgacttctt aaataacaat cttttagatg gacgagaaat
    13081 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
    13141 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
    13201 taacttgcca caagttattg ttcctaaaac aggttctaca catttagtaa cgattttagc
    13261 ggtcagctta ctattacttg tgttagcaac ttttggctac gcagttctga agttcaatta
    13321 aacaaaaaaa caaggctgaa aatcagcctt gtttttctat ttgaaaatgg gattggcact
    13381 aataaatacg ttgtctgaag aaagtgatcc gtctgcagaa acttcataag catgggataa
    13441 accaacgata ctttgattgt tgtaaagcaa gtttgcgtta tgagcagcgc tatctttcca
    13501 cataaccata gcatttactg ctcctgtatc tgaaaggatc tcgtcaacgt aaaggtagaa
    13561 actattgata ttttctcctt tagcaccgta gcagcctaag ctttctaaga gatcttggaa
    13621 agtaccgccg ccaggtcggt aatgactgaa gcttacttgt aattcttttg cacgaatatc
    13681 tgcagcttgt tgaacgatat cattgaattg cattggggca acgccatttt gttgacggta
    13741 gctatttaac caagcaagtt ctttttgacg cacatagttg tttaaatcac ttagtgagat
    13801 atttttgtat ttgaaagtga tcgttcgatt ttggtcaaca ttattgatta cacgttgtcc
    13861 atttttatca acggtaacag atgaatcgtc atttctacac aagaagtagt tttgatcggt
    13921 catatttttc caagaagtag agaaactatc tccctcgtaa accgtttgtg tttcagtaga
    13981 taaaacagcg tttgtgtcaa cgtttatatg gttcacagtg acgttgtatt gatgagtata
    14041 ggcaaaattg attccttgat tacttgtaac gttgtttaaa gtcacagtgt tgctagagct
    14101 ttctctagga tcaagaacaa aattagtgaa cataggtgct tcaattgtga tgctttgtcc
    14161 ataaacgacg ggataactct tactgtaatt atagagttct tggatatttg gatcagtatc
    14221 cgagacttta tttggacgag ctttcacttc aactagaaag acttcatcat agtaaaaagt
    14281 aaatgtggta tttgtgttaa tcgtttgtgt ttgtgtttca ttcccattta cttgatactg
    14341 tggtgcttca aagttagtca agtcggttct agcttttgca gtgtaacttg taccagcttt
    14401 tactgagaca tgttcttctt ttagcagcgt tccagtttgt cgattcatat gacggacagt
    14461 taccgcatat tctgaaggtt taggtttgtg ttgtaaccct ttaaatgcag aatcaagact
    14521 atttttagca ttatttactt gggtttgaga agctttagaa tcgtctagta cttttttagc
    14581 attggataaa gcagtctgaa aattattcca agatccttct gtaaaatctc ctttagcagt
    14641 tgattttact tggttgtaca aggcttgtaa ttccgttttg ttcacagtag gagctggttt
    14701 tttctttaat cccttgtaag cagaatctaa tgtgtttttg gcactatcca cttgtttttg
    14761 agtcgctttt gagtcattta acactttttt agcattgttt aaagcggttt ggaatgcgtt
    14821 ccaggtattg tcggtatagt cacctttttt agttccctta acgctcgtat aaagagcttc
    14881 taaagctgat ttattcactt tgggtttaga ggtgtcagca ccataccaac caataccttc
    14941 gtatttccag ccatagtctt tggttaacca ttgtacttct ttccaacttg cagtgtagtg
    15001 atgcgaacca gaggtaacac ctggaaggaa aaggcggtag atcggtttat tatttttttc
    15061 ggcagaacgc caggcaggcc cttcataagt ccaaccgtaa tttttggtca accatttcac
    15121 ttcatcccaa ctagctgtgt aatggtgatc acgaaggcct ttattgtata aacgatagac
    15181 aaggtggcca ccgctagtcg gtgcttccca agcggggcct tcataattac cccacttaat
    15241 tttcactagg tgatcacgtt cttttgtgct agaggtgtaa aaatgttcat ggttatttgg
    15301 attataaagg cgatacatga cgttagttga agcagcgtca gcagatccag caagagcaac
    15361 tgtggcagca aatgttccaa tagctataac agtttttggg tttaataatt tcatgttttc
    15421 aactccctat taaatgatta aatattttgt ttacattcat aatataccac ggtataccga
    15481 ggtagtcaac ggtataccgt ggaaattttg aaataaaaaa gccgccctat gaagagtgta
    15541 aggatggctg tttatatgct atgaagaaat ggattcggcg tatttggtta gtttggcagc
    15601 attatcaaat cgcatttttt caatcgcact ttttttcttt attaaatccg caattgttga
    15661 ttctgcgact gaagaatgtt tgctaatttc atatgctgtc ttgccagttt caaatagtaa
    15721 ccattcgatt ttctctttat cagcgtacat tgcaatgctc cctcttgatt ttttctttat
    15781 tatagctctt ttttgtaata aatcacaata tcaagaggaa aagtttctat agaaaggtgg
    15841 gaaattatga gtatccgttc cccgtgtatc aaaatgaggt ttgttgctac tttcgttatt
    15901 taatatacta aaataaaaca acgaaagaag gaatagaatg aaaaagttaa taatggtatt
    15961 tacttcgttg attgccatga ttgttatttt aggtggatgt ggtagtgaaa aaaataagca
    16021 agatagtcaa gataatggcg tgttaaaacc aggaacagtg tggaaagaag aagtaggcgg
    16081 acttgttcat aatttaaaaa ttatagatga tacaacatgg gaatattccg aaagtgtttg
    16141 gcatcctgaa caagtacaaa taacagttga gaagctaaaa gactataaag gattagaaag
    16201 atataaggta atcgattcag gtggagtaga tttattcata ggtaaaacag ccttaatagt
    16261 tccctataac aaagacggtt tagaaacgtt taaatttatt ccaacaaacg aaaaagaaca
    16321 tcgagataaa agtgaaatat taaaagaata cagtactaaa tctggataca agttacaaaa
    16381 gactagcgag tgattgctag tctttttatt tatcctgaag gaggaatcgc agtggcaaaa
    16441 gctaaaacgc catttgaaaa agccgcgtct aaaatcttag aacgtcgcgg tgtatctgaa
    16501 acacagtgga aacaagaagt ccttgaaaaa gcacaatttg acctattacg gggaaaagac
    16561 aaggacttag aaggatatat ctatcaacgt gaacgtgaaa agcttgtttt cgatgaaatt
    16621 attaaacact aaaaggagcg aacaatttta tgtttgaatc aattacaatg aattttttaa
    16681 cacaaccgaa agtagtagct gcggttgata tttttagagc attacaaggt gcagcaacta
    16741 gtttaaaaaa caatctgatg atttttgcgg ctgtagctgc cgttgcctcc attgttcttg
    16801 gggggatttt cttcttcttt ggccgaagag gagctgaaaa cgggaagatg cacgtaggtt
    16861 atgtattgat gggggtagga gttatattag ctgccgcctc tattgtggtt tcgttatttg
    16921 gaatgtttgg acaaacccca cagggactat aaaaaataaa tagaaggagt aaaaagtacg
    16981 tggctacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgatttcagg
    17041 caataccaaa aaagataaag agttaataaa aaaagcaacg aagaaagtgg gaaagcgtac
    17101 tgccttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
    17161 gttaggacaa gtgttagaac aagagaatca atcttctgtt aaattgactc tcacgttaca
    17221 tgataaagaa ggaaaaatag aaaaggctgg cacagaatac gtttttggcc cactggatta
    17281 tcaaatttca gaaggttttt atcctacatt gatggatttg atccgccttg atgtgtttaa
    17341 ggaaacggat gaaaccatga gtgtggaaga gtcttatcga aacgaaaaac tgctacaagt
    17401 tttagaagaa gagttagaga gcgaccaaag aaaagtgagt caagtgagtg aagtcgaagt
    17461 aaatgatgaa gtaccatggg aacgaccaat ctttccaaag aaggagtcaa aagaaaaaga
    17521 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
    17581 gaacgatgta atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
    17641 gacggccgaa gtagaagttg atctagttga agctaatgaa gagccaacat ggactgaagg
    17701 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagagaaaa acgaaacaga
    17761 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
    17821 gatgaaagaa aaaaataaat tatttttaaa tgagctttat tcgaaatatc aattacccca
    17881 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
    17941 tttagaacag ttagagtctg ttcaatcaac aaattggcat gagcttgcac aagaagattt
    18001 attagccact tttgaagaac gtcaacaaga aacagatcaa gaaataaatg agtatgcgac
    18061 ggaacagtca tctaattggg agagaacaaa agaggaacta aataaagaag aacaacgagt
    18121 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
    18181 ttccttgtgt atagagaaaa ttaataactt tactaaaact aatcaagaag ctttatcgaa
    18241 agaaaaagaa aacttgttaa aagaacgtat aaaagaacga aaagaagagt tctaccgagg
    18301 tttacgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataaacttta
    18361 tcaaacaacg actgaagttc ttatggaaag acacgggcag atccagcaag aattacaagc
    18421 acaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    18481 agaaaaacaa cgacaagaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgagca
    18541 gcatcttgca gaacaaaaag tggcgataga aaaacaacgg ttagagcaag aaaaggaaaa
    18601 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagcacagtt
    18661 gaattatcta gcacaacctc aaccagtagc tgctgttcaa acgccagtga acaaccaagc
    18721 aaatttaact ccttcaccac agcgttcctt taacggatgg atggtgggat gtattgcaag
    18781 tttaagcttg cttctaggtg gaggaacagt atttgctttt aatcatatca atcaatcttc
    18841 acaggcagat tctgtagcca caacggcgtc aatcaaggaa gaagcccagg aacaagcaag
    18901 aaaagagtat gaacaacaat tggaagaatt attgaatcgt accaatcagt caacagcaga
    18961 gtcaagtatg gaaaatagca aagagagtga tcctagtaag gctaaagaag gatcaagcga
    19021 ttcaactgta cactcaaaca cttccgaaag ttcagaatca aatgaggtga aataatgcgt
    19081 attagagaaa caacgagacg aaaagacaaa cgattcaatt tagaaaagaa taggtatctt
    19141 tctcatttct atcgctatac tccttcagga gaagagctac cagaaaaaca tgacaaacgt
    19201 atttctcaaa agatttttta tagtggctta gcttatcttc ctttaaacta tgttgttaca
    19261 agaataccag aagcatttca tcgatttgag attattccag gaagtttaca aggagtgaat
    19321 atttttcagt tacaagaaat gccgcagtta ttccattttg attcaactgg tttttggaat
    19381 cctttgaccc caacaacaag tttaggcaca cgtttagttg tttctacgat ttgtgttgtt
    19441 ccagctgtac ttatgggatt gaaaatggac atgtggtttc gtccgctagc agatgggcaa
    19501 aaaggagata accgattaaa aacgttaaag gaaatccaac aaatttatcc gcaaatacca
    19561 gaatcaaagg gaagttttcc tggttatggc ggaataccaa ttactcatta taaaaaatac
    19621 tggtatattc agacggatac agtaaatact tgtattgttg gtacttcccg ctcagggaaa
    19681 ggccagatag aagttttagc aaccattgac aatttgtcgc gtgcagaaag acaaagttca
    19741 atggttgtta atgatccaaa aacagaatta tatgttgcta gtaaagatga actagaagct
    19801 agagggtatg atgtgtatgc ctttaatatc accgatccac tgcaatcaat gagtgataat
    19861 ccattggcac tcattgtgaa atactggaag cgaggagaca tagacaccgc aacacagtta
    19921 accaatactt ttacgaatac gatttattat gatgcaaatg cttcagacaa taaatacttt
    19981 aacgaaaatg cgcagaaagc tgtcaatgcg cttatttttg cgttattgga acaagcggat
    20041 caaacagggg attactccaa attaacacca aataatctcg ttgaactact ttcagaaatt
    20101 ggcggtttta attattatcc agatcctgag aatgaattca agcagctcaa tgccttagat
    20161 gagttcatga atcagttacc ccctggaaat atagcaaaaa aacaatatgg ggcaacaaaa
    20221 attggatctg ataaagcgaa agggaatatc ttgtctacgg caattacagg attgaatcca
    20281 tttaccctaa cgaaaattgc gaagatgact tctcaatcga ccattgatta caagtccatt
    20341 ggttttccga agtatttgga tctcaaatta catgaaagcc tattaaatca acgcattgtc
    20401 gtggaatttt atcataaggg aaagaaattt cacgaggaac gagtcaaagt tggctatcgt
    20461 ggtttttgtg aaatcaactt tgattgtcat ttagagcagg gagatcatgt ttgcttaaaa
    20521 tacaatcatc aaggaaaaca atatgttgct tggtatgagt tttctcaaaa agtattgaaa
    20581 gacgaagagg gaaaaatcgt ttatcgaaaa aaacgaggag aaactcattt gccagaacta
    20641 gaaaaagaag ccgttctaac attagatgag gaaaaaagta acttattagt agagtctatt
    20701 cggatgcact ataccgataa accaatggct atttttatgt taacgcctga ttatgatcca
    20761 tctaaccata tcattgttag catctttctt agtcagttgt ataaagaatt atctacgcaa
    20821 tgtgttaaga ctaaaggaga taaatgtcac cgtaggattc attttatttt agatgaattt
    20881 ggaaatatgc caccgatgga caacatggaa ggaatcatga cggttacggc aggtcgtaat
    20941 atgttatggg atctgtttat tcaatcttat cagcaacttt atgcgaagta tggagaacaa
    21001 aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc gacagatgat
    21061 aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca agagaacagt
    21121 accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc agatcgtatc
    21181 ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact cgctccttta
    21241 aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa tcatggggaa
    21301 acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg ggatatcaat
    21361 gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca aattgattat
    21421 gattatttcc taccagatga atatgctaga aatctctatc atggactgca tgatcaatta
    21481 gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatcttttga gaatgagacg
    21541 gctaaaaaat tgttaattca attttacgaa gaacaagcaa cggatcgtct tcaagaatat
    21601 gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttaggaaacg gctgttattg
    21661 gaagctgaaa aggcggtgaa agaatgatca ttggaggaat tttctctggc atagccgact
    21721 ggtggtcagg tttattcgga gaaggggatg cagatcccaa aaccgtaacg aaagtcttac
    21781 aagattatgg taattatctg cagtatcaag atgtattcag ttggatttgt aatacttttt
    21841 tatggggatt gatcaaaggg ttatataatc tcaatacttt attagaaaaa acaatttatc
    21901 aaagctttga tttaaaagac ttattgaacg ccgctggtgt taatgaacta tttcaaagtt
    21961 tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttgaaat
    22021 tttttatgag taaacaccca ccgaaagtca aaaatatctt ggttaattta tttatggcca
    22081 tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc agtaaaaact
    22141 tttatggaga cgtcactgca gcaaataaag cagataaaac gggttcacct gcttttcaaa
    22201 ttattcaaaa taatgtgatt gatgtcagta cattactagc gactgacccg aataaagtag
    22261 aatccttacc aacagataaa agaaatgctt taacagcgaa aaatttcaaa acagcgaata
    22321 tcaatgggat tattacacct gatcaagcta agaaaatgtc tgaatcaagt gatattagta
    22381 cagtacaaaa agatcggtta aaggcattac agtatcgact agagttagac gataatggga
    22441 aagaaatccc tgttcaaatt agtgatgaag gtttagcaaa atatgtgtat acgtcaggct
    22501 atcgtcgata cattagtagt ccagggatta ttttggctgg ggaatcttct ttgtcggtag
    22561 cctatttatt tattttgttt acaatcgtta cttgcattat tgagctagta tttaaaaaat
    22621 tctatttagt aattgccgcg tcaacggact ttgagacagg acaacggatg aaaagtgcga
    22681 tagaagatat ttctcaatcc ttcttgttat tggcgtttac gattttagaa ttacgaatct
    22741 atacattaat gctttcaggt atcggtgatt tgcatgcggt aggaaagatc aatggattct
    22801 tgtatgttgt tgctttaatt gtgctaacta ttgcgctatt caaaggctct caatcggtta
    22861 cgaagatttt cggtgttgat acgagtctga aaaacggcag caattctctt cttagcatgt
    22921 ttgccttagg aaatattgcg aaaaatgtgg gtagcggagc taaaaacctt gcaagtgctg
    22981 gtaagagtgg tttaagccat ttaaatgata ttcgtaagaa tggatttggc catcgaaaag
    23041 atattaccca tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata
    23101 gtaagaagac agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag
    23161 aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac agttgaagat cttacagaaa
    23221 aagccaaaga caccttagaa gagaaattag acggcacgag agctaaagaa tttgctgaag
    23281 ctgtgaagaa tccatccgaa gcactagaaa aacaaaaaga aaaagcttca gcagtcaaag
    23341 aaggactaaa agatgcgtat ggtgaaggac aagtcaatgc agcagtgaag gcaaatacaa
    23401 caagtgaaaa agaaaatcaa gaaggtgcag cgattccttc agccaaagat caccaagatg
    23461 agttacattc taacgaaaat tcgatcggag aaaaagattc agaagaagga caacatggtc
    23521 agttaccaaa aaccgatcca gaacaatatc gttataaatc taacattaat tctgaaaacg
    23581 aaaaacgcat ttcagattta ccaaaacgaa atcaagaagt acaaacggat gaggattcca
    23641 tcgatctgcc aaatgataat ggcatcgaag ttggaactga aaaatcaacg aaaattggtt
    23701 tgcctaagac acaaaattta ccaatgaaag atagtcgttt gaatcaattc aaagagaatg
    23761 atcaaagctt tgttcatcaa tc
//
LOCUS       DACCSK010000037        23353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCSK010000037 DACCSK010000000
VERSION     DACCSK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.037"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IVP39_001255"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IVP39_001255"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7586335.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IVP39_001256"
     CDS             complement(1173..1868)
                     /locus_tag="IVP39_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7586336.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHAELYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IVP39_001257"
     CDS             complement(1881..2597)
                     /locus_tag="IVP39_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7586337.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTSADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3535
                     /locus_tag="IVP39_001258"
     CDS             2927..3535
                     /locus_tag="IVP39_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7586338.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3862..4185
                     /locus_tag="IVP39_001259"
     CDS             3862..4185
                     /locus_tag="IVP39_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7586339.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4367..5737
                     /locus_tag="IVP39_001260"
     CDS             4367..5737
                     /locus_tag="IVP39_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7586340.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5867..7168)
                     /locus_tag="IVP39_001261"
     CDS             complement(5867..7168)
                     /locus_tag="IVP39_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7586341.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALVAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7522..9513)
                     /locus_tag="IVP39_001262"
     CDS             complement(7522..9513)
                     /locus_tag="IVP39_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7586342.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9657..9923)
                     /locus_tag="IVP39_001263"
     CDS             complement(9657..9923)
                     /locus_tag="IVP39_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7586343.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10433..12559)
                     /gene="pnp"
                     /locus_tag="IVP39_001264"
     CDS             complement(10433..12559)
                     /gene="pnp"
                     /locus_tag="IVP39_001264"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7586344.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12747..13016)
                     /gene="rpsO"
                     /locus_tag="IVP39_001265"
     CDS             complement(12747..13016)
                     /gene="rpsO"
                     /locus_tag="IVP39_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7586345.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13267..13830
                     /locus_tag="IVP39_001266"
     CDS             13267..13830
                     /locus_tag="IVP39_001266"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7586346.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14073..14594)
                     /locus_tag="IVP39_001267"
     CDS             complement(14073..14594)
                     /locus_tag="IVP39_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7586347.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14597..15439)
                     /locus_tag="IVP39_001268"
     CDS             complement(14597..15439)
                     /locus_tag="IVP39_001268"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7586348.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEEPLEESLETLRQKIDTIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15432..16157)
                     /locus_tag="IVP39_001269"
     CDS             complement(15432..16157)
                     /locus_tag="IVP39_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7586349.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16333..16629
                     /locus_tag="IVP39_001270"
     CDS             16333..16629
                     /locus_tag="IVP39_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586350.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17197..18789
                     /locus_tag="IVP39_001271"
     CDS             17197..18789
                     /locus_tag="IVP39_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7586351.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18802..19542
                     /locus_tag="IVP39_001272"
     CDS             18802..19542
                     /locus_tag="IVP39_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7586352.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19710..21059
                     /locus_tag="IVP39_001273"
     CDS             19710..21059
                     /locus_tag="IVP39_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7586353.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21187..21777)
                     /locus_tag="IVP39_001274"
     CDS             complement(21187..21777)
                     /locus_tag="IVP39_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7586354.1"
                     /translation="MTKSRLEAFTDGVVAIVLTVLVLEIKIPNPSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21924..23276
                     /gene="brnQ"
                     /locus_tag="IVP39_001275"
     CDS             21924..23276
                     /gene="brnQ"
                     /locus_tag="IVP39_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7586355.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVDQAPIQPNYQDHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPILMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLHVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6668 a         4722 c         4457 g         7506 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagacg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttggatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgtcct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag ttcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ctgatgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagttatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 agaaagcaca ggtgaaggtg gcgaaccaga agtaatcggt tcaacagaag aataatactg
     3541 aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc tttttttctt
     3601 tctaatcgtt tattttcata tgaggcttcc acttttacaa atagctttgc aaacgcaaaa
     3661 atcctaattt accaagcttt tcctcgcata cttttatcgt acctttataa aaatttatac
     3721 tttgaaaaaa agtattcaat tattgttgca tttttcctga ttctttatta gaatgaaccc
     3781 tcgttgaagt aagtatttct agtttatcct aggattaatt ataaaaataa aacattcgtt
     3841 gattcgaagg aggttaaaca gatgtttgaa agttttgata gcagccgcaa ccgttacgct
     3901 tcagttggtg tcgtatcaag tttgccagat gaattgattg acagtatatg gtttattatt
     3961 gacctagatc ttaagggagt cattccatta gaaaatatat taacctttga tttgatcgaa
     4021 aatcgtggaa aagtgacgat gcatttttct caagcaggaa gcaatgtgga aatggatatt
     4081 gatttgccgt ttgcctattc gaaccaattt cctgctcaag tatacgccta tgatgacggt
     4141 acgagagaaa cgatcctttt accaagtgag atcagacaat attaaggtga tagataaacg
     4201 tggatgtccc ttattttaca gaccaggcca agttgttgat tctttgactc aattcttggc
     4261 ttctttttat ggagtttcat aaattatcat tgatttctaa ttggtattct caggataaac
     4321 gctatatact actaactaaa ttttaaagat ataaggagtt taaaagatgc gcgatttaac
     4381 aaaaggaagc cccgctaaac tgattttgat gttcactgtt cctttactga tcggaaatgt
     4441 ttttcagcag ttctacaata tgatcgatat gatcatcgtg ggtcagacgc taggtaaaaa
     4501 tgcgctggca gcggttggtg caactggaag cctgacattt ttgatcattg gctttgccca
     4561 aggtttaaca gctggtttag ccattattac cgcacaacgt tacggtgcaa aagattatcg
     4621 gggtctgaag aaaagttttg ctgctagtgt tgtcattagt cttgtcgtca ctgtgatttt
     4681 aactgtccta agtctcctgt ttattcgtcc gatgcttcaa ttgatgcaaa ctcctcctga
     4741 aatacttgat caagcacaaa catttatttc aattatttta ttagggatct ttgcatcagt
     4801 cagtttcaat ttgctttcta atgtcattcg cgcacttggt gacagccgca caccactatt
     4861 ctttctgatc attgcggtca tcatcaacgt cgtacttgat ttgatcttta ttatctattt
     4921 tggcatgggt gtggaaggtg cagctattgc tacagtaatc gcacaagttt cttccagcgt
     4981 actgtgtcta gtgtatatca agaaaaaaat gcctttgtta cagttacgca aaaaagattt
     5041 caaatttgaa aaagaagagt ttgctgtaca cctaaatgct gctttgccaa tggcgtttca
     5101 atcttcgatc atcgctgttg gtgctatcgt gttacaagca gcactaaata gtctagggac
     5161 agatgtcgta gctgctcaag ccgctgctgg tcgtatcgac caatttgcaa atcagccgat
     5221 gatgtcattt ggtattgcaa tggcaacttt cactgcacaa aactatggtg cgaaagaata
     5281 tggccgaatc ttgcaagggg tacgtcaaac tttactgatg agtgtcggat ttagtctctt
     5341 agcaggtgct atcgtcattt tcttcggtca tttctttgtt caattgtttg ttagcccatc
     5401 agaaacacgt gtatttgaat tagcacaaac ctacttcaat atcaatgggt ctctttactg
     5461 gatcttagca gtcctgttca ttttacgcta cacacttcaa gggcttggac aaagcaagat
     5521 ccctacgatc gccggtatga tggaattgct tatgcgttct tttgcagcta tcgttcttac
     5581 aggaatgtgg ggatacccag gagcagcagc agcttctcct ttagcttgga tcggttctgt
     5641 tgccgtgttg ctttactctt atatgcgttc gatgaaacaa ttgaaacaca tgggtgaaga
     5701 ggaacatttc acattagaac atgcagaagc aagatagcct tgattaaaag aaatcgttgc
     5761 ggctaaaaat gaaaactttt attgaaaata tcttcttcgt ttaccgaaaa caggtttttt
     5821 agataacaaa aaggctgtga caaaaaatgt cacagccttt tttcttttat tctgtttttc
     5881 tttccgtgga ctctactttg aaataatcga tatgtgcatg ctggtattct aataccgtga
     5941 aacggaatga tgcaatctca atgattgttc ctgcttttat ttctttatat cggctagaag
     6001 caaagcccgc aagtgtagta aaattcgttt cttcaaactc tggaatctct acatgaaaat
     6061 aacgctggaa atcatctaat gttagtttgc cggagatgat ataagagcca tctgactgtt
     6121 ccgctaccaa tgcttcgtcc aaatctgtct catctcggat ttcgccgacg atttcttcta
     6181 aaatatcttc aatcgtcact aagccgcttg tccctccgta ttcatctttt aagatagcaa
     6241 tatgctttcc cttttttcgc atgatcgtca ataatttttt tatagggatc gtttcagttg
     6301 ttacgatcgg ctcttcaatc aactgttcta ctgtcagctg gtcatcttta taagattgct
     6361 tgatcaaatc tggcaaggtg acataaccta aaacattgtc ttttgattct aagataaccg
     6421 gatagcgagt atgtccttgt tcaatcgaag cttttatcgc ttctttgacg gtttctgaag
     6481 cttcgaatac ttgcatggac gtacggtcta cttgtatatc ttttgccaag gtatcatcaa
     6541 attcaaacac gttttccaaa taatgatatt cttctttggt caactctcct ttttcataag
     6601 aattcaatgc gactaatgtc agctcttctt gagacaatgt ctcttctcct tcagatacga
     6661 gacgcacacc taatagcttc cctagccctg cagctgaatg attcaatacc caaataaaag
     6721 gaaacattac tttataaaaa taatgaagtg gtttaactac gaacaagacg accttctccg
     6781 tattgacgat actatagctc ttaggaagca gttcgccaat aaccacatcg atatacgtaa
     6841 ctaaaataaa tcccagcgcc acagagattg tactgctaat agccgaacta aaaggtagtt
     6901 taccgatcac tggttctagt aaagccgcga cagatccttc accgacccac ccgatgatca
     6961 aaccggttag cgtaattcct aattgactag ctgataaata gttatctaaa tggtgtgtaa
     7021 catgaagagc tagcttggcg ttctttacat tttccttttc tagttgttcc aatcttgatc
     7081 gtctgatttt cactaaagca aattcacttg ctacaaaaaa tgaagtaatt gcaatcatca
     7141 aaaataaaat aatgaaattg ataaccatat tctcccgcct ttctcttttt ctattgtacc
     7201 ctttttctac aaaaagattg aaaataatgc gtattcatat ttaggaataa aaaaggagat
     7261 gcggcatttt tcagccacat ctccttatca cttcaaaatc attgcgttac ttgttgctaa
     7321 acagcttttt catgacctct gataaaaacg gtattcaaaa gcttgcatct gcggcattaa
     7381 gcccaaacag atgaaaaaca tgagaagctg tttttatctg ctcgttctta ctgcttgttg
     7441 cagtacagct ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta
     7501 gttcaggtca aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc
     7561 aatgataagc ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc
     7621 tggcgaggta gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt
     7681 tcctagagtt gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac
     7741 ttctcgccct ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat
     7801 caaatcatgg atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa
     7861 gaacagattc acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt
     7921 atatggctca tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg
     7981 tttatctaaa atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac
     8041 caagaagacc acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag
     8101 ctgcgggata taatccctca aagacacatc ctcggttgac gattcgcgaa taatgttccc
     8161 ttgttcccaa ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc
     8221 gcctcggaag aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa
     8281 aaaggtaact aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag
     8341 cagtgctgtt gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg
     8401 atgagacgtt cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac
     8461 tgctgggata tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag
     8521 cagtttcaac ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga
     8581 agcaatgact gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat
     8641 catttggaag aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt
     8701 caacaaccac gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca
     8761 gctcagacga atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc
     8821 tgtcgtagcc aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa
     8881 aagcagctgg atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa
     8941 gttcattaat cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc
     9001 ttttccgaca atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa
     9061 agtgataatg acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc
     9121 ccgcaagcct tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag
     9181 taaggcagct cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag
     9241 tgtgtagagt gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac
     9301 atatttgatt gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac
     9361 aaaatcagga ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta
     9421 aagcggactt gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat
     9481 tgatatcttt ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg
     9541 atatccttta ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct
     9601 aaattgcttc taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat
     9661 tctttctttg gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa
     9721 attgcaccca ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct
     9781 agtaaaaatt cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct
     9841 cccgggactt cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat
     9901 aacgaccata aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat
     9961 tttacgcttt attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa
    10021 gttgtaacaa aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca
    10081 gaggttgtga aatcagcgat ttgacctgag tattagagaa ataaacggca aaatagttcc
    10141 tcatattttg ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg
    10201 gagaggttgt aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct
    10261 cctaatgttt ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt
    10321 attcgcaaaa aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta
    10381 ttgtccccct gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt
    10441 cttcattttt cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc
    10501 cttgtttatc gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt
    10561 cgactttatt gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt
    10621 tgatcaagtt gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt
    10681 caccaacttt gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt
    10741 ctgcatcagc tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag
    10801 tttcatcgat gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag
    10861 caggttcgat ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag
    10921 cagcaatcgt tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta
    10981 atgcttcagt taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag
    11041 tgataccatc ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc
    11101 cttggatatc agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac
    11161 cggcgactgg cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga
    11221 tacttgcttg ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat
    11281 aaggaaattc ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat
    11341 gtccgatttc ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag
    11401 ggaaattgta gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt
    11461 ggtgctctcc tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga
    11521 acaaacctga tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt
    11581 catccaattt acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat
    11641 ctttttcaag atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat
    11701 gacctgcaaa tttctcagca taagcttcgc ggatatcgat cttcacttgc tcgatattat
    11761 cttcgcgagc aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat
    11821 caaagatttc ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt
    11881 tccctactgc ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac
    11941 caaacagcaa tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca
    12001 tattgatggc ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt
    12061 ctactgttgg attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg
    12121 ggccgtcaaa tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca
    12181 tagctggcga gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac
    12241 ggaagccttc tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg
    12301 cttccgtact tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat
    12361 acattttttc ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag
    12421 aggccacagc tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg
    12481 cttgttttgc tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga
    12541 atacttgttt ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa
    12601 acaaatgaaa actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc
    12661 atctagcttg atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat
    12721 gtgaatccca ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg
    12781 cggtaacgtt gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact
    12841 tttttcatta ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc
    12901 aagtggttga tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct
    12961 tcatggcgag cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct
    13021 ttcacctctt tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa
    13081 gtggcaggtc atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc
    13141 aagtagttaa tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt
    13201 ttgtggtaaa ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca
    13261 tattccatga ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt
    13321 gcaaaagaag tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt
    13381 gagttcttaa aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt
    13441 gttggcttag ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca
    13501 agtcctgacc cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt
    13561 ttgagccatt ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat
    13621 cgagaagtac caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg
    13681 aatggtgaaa aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa
    13741 atcgaccata tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca
    13801 ctaaaagaag gcgtattggt aatcgagtag agaattggca ggttccttcg atactatgct
    13861 acaagaaagc tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt
    13921 ctccactaat cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta
    13981 ttggatacgc atcttttctt attttttcca tatgggtgtt tatctcagag atgatagaat
    14041 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    14101 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14161 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14221 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14281 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14341 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14401 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14461 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14521 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14581 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14641 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14701 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14761 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14821 ctcgatcgat cgtatcgatc ttttgacgta atgtttcaag agactcttct aacggttctt
    14881 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14941 atttggtcac aactgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    15001 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    15061 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15121 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15181 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15241 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15301 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15361 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15421 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15481 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15541 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15601 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15661 actccatctt gaaatccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15721 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15781 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15841 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15901 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15961 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    16021 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    16081 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16141 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16201 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16261 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16321 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16381 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16441 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16501 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16561 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16621 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16681 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16741 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16801 aaaaacagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16861 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16921 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16981 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    17041 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    17101 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17161 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17221 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17281 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17341 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17401 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17461 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17521 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17581 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17641 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17701 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17761 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17821 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17881 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17941 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    18001 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    18061 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggcgc tggacttacc
    18121 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18181 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18241 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18301 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18361 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18421 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18481 acaatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18541 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18601 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18661 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18721 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18781 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18841 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18901 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18961 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    19021 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    19081 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19141 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19201 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19261 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19321 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19381 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19441 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19501 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggataaata gctgattggg
    19561 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19621 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19681 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19741 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19801 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19861 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19921 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19981 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    20041 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    20101 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20161 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20221 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20281 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20341 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20401 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20461 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20521 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20581 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20641 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20701 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20761 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20821 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20881 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20941 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    21001 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    21061 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21121 ttcttaaatt tttgtgaaat ttcggcttat ttcttggagt taaaagcttc tgtctcagcc
    21181 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21241 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21301 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21361 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21421 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21481 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21541 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21601 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21661 gatgacccat aaagaatgaa agctagatgg attagggatc ttgatctcta atacaagaac
    21721 tgtcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21781 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21841 gttttatata ggaaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21901 aaaaaaccct aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21961 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    22021 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    22081 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22141 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22201 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22261 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22321 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22381 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22441 cattaggctc tgtcgatcaa gcgcctattc agccaaatta ccaagaccat gcttttttca
    22501 caggatttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22561 tcatcgttac gacgatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22621 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22681 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22741 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22801 tattagcttg tttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22861 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22921 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctatt ttgatgttca
    22981 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    23041 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    23101 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttacatgttg
    23161 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23221 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23281 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23341 ctcacatttt tgc
//
LOCUS       DACCSK010000038        23067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCSK010000038 DACCSK010000000
VERSION     DACCSK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.038"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            493..1200
                     /locus_tag="IVP39_001276"
     CDS             493..1200
                     /locus_tag="IVP39_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7586356.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IVP39_001277"
     CDS             complement(1259..1903)
                     /locus_tag="IVP39_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7586357.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IVP39_001278"
     CDS             2030..2317
                     /locus_tag="IVP39_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586358.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IVP39_001279"
     CDS             2425..2745
                     /locus_tag="IVP39_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7586359.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IVP39_001280"
     CDS             2826..4139
                     /locus_tag="IVP39_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7586360.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IVP39_001281"
     CDS             4136..4855
                     /locus_tag="IVP39_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7586361.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IVP39_001282"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IVP39_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7586362.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IVP39_001283"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IVP39_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7586363.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IVP39_001284"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IVP39_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7586364.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IVP39_001285"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IVP39_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7586365.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IVP39_001286"
     CDS             10292..11185
                     /locus_tag="IVP39_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586366.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IVP39_001287"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IVP39_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7586367.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IVP39_001288"
     CDS             13122..13979
                     /locus_tag="IVP39_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7586368.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IVP39_001289"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IVP39_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7586369.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IVP39_001290"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IVP39_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7586370.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IVP39_001291"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IVP39_001291"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7586371.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IVP39_001292"
     CDS             16735..17493
                     /locus_tag="IVP39_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7586372.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IVP39_001293"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IVP39_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7586373.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="IVP39_001294"
     CDS             18406..19437
                     /locus_tag="IVP39_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7586374.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="IVP39_001295"
     CDS             19434..20201
                     /locus_tag="IVP39_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7586375.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="IVP39_001296"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="IVP39_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7586376.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQCQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22048..22353
                     /locus_tag="IVP39_001297"
     CDS             22048..22353
                     /locus_tag="IVP39_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7586377.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            22353..22703
                     /locus_tag="IVP39_001298"
     CDS             22353..22703
                     /locus_tag="IVP39_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7586378.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            22841..>23067
                     /locus_tag="IVP39_001299"
     CDS             22841..>23067
                     /locus_tag="IVP39_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7586379.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAH"
BASE COUNT         7597 a         3971 c         4964 g         6535 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagt gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 ttatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat
    22801 tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac
    22861 catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat
    22921 cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca
    22981 aggtaagact gctcttgatt attggcacca gtataaagaa aataagaaaa aatgtgggag
    23041 aaaagtcatt caattacctg ctcatga
//
LOCUS       DACCSK010000039        22992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCSK010000039 DACCSK010000000
VERSION     DACCSK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.039"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IVP39_001300"
     CDS             <1..411
                     /locus_tag="IVP39_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586380.1"
                     /translation="AELLDKDKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRT
                     VDNFYVCGLNTLLDGIKIPWKKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVY
                     KTKYMNLFVSGSYIGEGIVVLDHKWEEGTHSNEI"
     gene            401..1987
                     /locus_tag="IVP39_001301"
     CDS             401..1987
                     /locus_tag="IVP39_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7586381.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            2026..4164
                     /locus_tag="IVP39_001302"
     CDS             2026..4164
                     /locus_tag="IVP39_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7586382.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(4532..5515)
                     /locus_tag="IVP39_001303"
     CDS             complement(4532..5515)
                     /locus_tag="IVP39_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP7586383.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            5893..7284
                     /locus_tag="IVP39_001304"
     CDS             5893..7284
                     /locus_tag="IVP39_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7586384.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            7300..8340
                     /locus_tag="IVP39_001305"
     CDS             7300..8340
                     /locus_tag="IVP39_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7586385.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            8359..9444
                     /locus_tag="IVP39_001306"
     CDS             8359..9444
                     /locus_tag="IVP39_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7586386.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            9477..10439
                     /locus_tag="IVP39_001307"
     CDS             9477..10439
                     /locus_tag="IVP39_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7586387.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            10432..11859
                     /locus_tag="IVP39_001308"
     CDS             10432..11859
                     /locus_tag="IVP39_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7586388.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            11861..12931
                     /locus_tag="IVP39_001309"
     CDS             11861..12931
                     /locus_tag="IVP39_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586389.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            12918..14339
                     /locus_tag="IVP39_001310"
     CDS             12918..14339
                     /locus_tag="IVP39_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7586390.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            14352..15359
                     /locus_tag="IVP39_001311"
     CDS             14352..15359
                     /locus_tag="IVP39_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7586391.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            15371..16375
                     /gene="neuB"
                     /locus_tag="IVP39_001312"
     CDS             15371..16375
                     /gene="neuB"
                     /locus_tag="IVP39_001312"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP7586392.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            16372..17520
                     /gene="neuC"
                     /locus_tag="IVP39_001313"
     CDS             16372..17520
                     /gene="neuC"
                     /locus_tag="IVP39_001313"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7586393.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            17493..18170
                     /locus_tag="IVP39_001314"
     CDS             17493..18170
                     /locus_tag="IVP39_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7586394.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            18160..19302
                     /locus_tag="IVP39_001315"
     CDS             18160..19302
                     /locus_tag="IVP39_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7586395.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            19315..20292
                     /locus_tag="IVP39_001316"
     CDS             19315..20292
                     /locus_tag="IVP39_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7586396.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            20292..21200
                     /locus_tag="IVP39_001317"
     CDS             20292..21200
                     /locus_tag="IVP39_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7586397.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            21201..21953
                     /locus_tag="IVP39_001318"
     CDS             21201..21953
                     /locus_tag="IVP39_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7586398.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            21958..22905
                     /locus_tag="IVP39_001319"
     CDS             21958..22905
                     /locus_tag="IVP39_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7586399.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         8151 a         3174 c         4263 g         7404 t
ORIGIN      
        1 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
       61 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
      121 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
      181 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
      241 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
      301 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
      361 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
      421 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
      481 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
      541 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
      601 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
      661 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
      721 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
      781 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
      841 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
      901 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
      961 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
     1021 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
     1081 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
     1141 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
     1201 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
     1261 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
     1321 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
     1381 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
     1441 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
     1501 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
     1561 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
     1621 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
     1681 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
     1741 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
     1801 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
     1861 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
     1921 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
     1981 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
     2041 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
     2101 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
     2161 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
     2221 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
     2281 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
     2341 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
     2401 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
     2461 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
     2521 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
     2581 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
     2641 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
     2701 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
     2761 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
     2821 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
     2881 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
     2941 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
     3001 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
     3061 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
     3121 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
     3181 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
     3241 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
     3301 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
     3361 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
     3421 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
     3481 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
     3541 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
     3601 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
     3661 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
     3721 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
     3781 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
     3841 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
     3901 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
     3961 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
     4021 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
     4081 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
     4141 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
     4201 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
     4261 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
     4321 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
     4381 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
     4441 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
     4501 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
     4561 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
     4621 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
     4681 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
     4741 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
     4801 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
     4861 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
     4921 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
     4981 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
     5041 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
     5101 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
     5161 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
     5221 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
     5281 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
     5341 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
     5401 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
     5461 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
     5521 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
     5581 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
     5641 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
     5701 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
     5761 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
     5821 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
     5881 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
     5941 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
     6001 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
     6061 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
     6121 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
     6181 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
     6241 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
     6301 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
     6361 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
     6421 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
     6481 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
     6541 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
     6601 agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
     6661 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
     6721 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
     6781 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
     6841 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
     6901 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
     6961 tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac
     7021 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
     7081 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
     7141 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
     7201 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
     7261 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
     7321 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
     7381 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
     7441 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
     7501 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
     7561 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
     7621 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
     7681 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
     7741 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
     7801 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
     7861 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
     7921 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
     7981 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
     8041 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
     8101 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
     8161 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
     8221 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
     8281 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
     8341 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
     8401 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
     8461 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
     8521 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
     8581 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
     8641 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
     8701 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
     8761 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
     8821 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
     8881 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
     8941 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
     9001 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
     9061 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
     9121 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
     9181 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
     9241 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
     9301 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
     9361 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag
     9421 agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga
     9481 aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc
     9541 gattgaacga aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat
     9601 ttattgaagc atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta
     9661 aaatttattg gggtggtttt tccattgtac aagcagaaat caatttagta aagagagcat
     9721 tacaaaatga gaaatattta aaatatgtat tattatctgg tgctgattat ccaatcaaag
     9781 ataatgagta tatttataat tattttaaaa agaatagttc agtggagttt attcgtggaa
     9841 tagatctaga tcaaattaaa cataaagaat tttattataa acatatagat gtatatcaaa
     9901 aacatgatta tcctagaatt aataggaata acacaactgc atttaaaatt tttagagcta
     9961 taattaatcg ttgtctgagg atgattaaac ttcccccaaa aattagacac cataaatttg
    10021 atttgtatca tggttctcaa tggtgggctt tgtcgaaaga atgcctaaca gaattaatac
    10081 aaatgtatga acaaaatcaa caagactacc tgaattttaa aataggtatg tttgccccag
    10141 atgaaaaatt ttttcataca ctttttttta attcctcgtt caaaaataaa aatgttattg
    10201 gaggaccaga tttaccactt gaattgaaaa atattgaaga aactacttta caaacttcga
    10261 aattagcaaa tattcatatt attgatcctt ccatgaacaa atggtttgat gaaagggata
    10321 tagatcaaat aaaagaaagt gaaaaattat ttgtaagaaa agttaaatcg gattactctg
    10381 ataaactatt aaatagaatt gataaagaaa ttttgaaagt tggaaatata gatgaataga
    10441 atagtacctg ttttagagaa gagtttatta agtcgagatg cgtttcgagt aataaattta
    10501 atcatttgtc tgctttattt tatgcctagt acttttaatg taactaatat cccgatgaaa
    10561 atagcttttg cttggggtgg aattatagtt ttgtatgagg tttttataaa gaaaaatttt
    10621 tttaaaatgc gtttttcttg gctactgatt ggagcaatga ttagttttat cttaactata
    10681 atcattaaca gagaaaattt tttaattcca tctatttata attttggata tttgctgatt
    10741 actttgatag taatttttcc tgcagacttt tcacaaagtg aagaggaaaa aaagaaaaaa
    10801 atggtgtatt ttaataatgt tttcattatc ataatttttt tagctgcgct aatatctatc
    10861 tatcaattta tttttttgat ttcttatcat gtcccaacag gaacgccagg attacttgca
    10921 agacaaggtt ttattgaaca tcggttattt ggtgtctata caagtccaaa tgttggagct
    10981 atgtttggat atacttctat tattttaagt ttaattagtg tattaatttc aaaaataaca
    11041 aaaacgattc gtgtttttta tatgatgaat tttatcgtac aaatatttta tattacactt
    11101 tcttcgtcta gaggtgccca ggtagtaatt tgttcttttt tagtattatt tcttttgttg
    11161 tttgccaatt caaaatttag aaagagcatt agagaagtac tacctgtaaa aatcaaatgg
    11221 atggtaagca tattattagt tctattggta tatttttctt ttggtactaa ttatgctaag
    11281 gattttttag caacaattcc tgtatcgatt gatagaaaag tagaagttat agagtcaaat
    11341 gaatcagata aaactccact tccagaaaaa gaagtaatat tacagcattc tagtgataac
    11401 tccgaaattt cagctggtcg ttttacgatt tggaaagctg cgcttaaagt tataagacca
    11461 actttgttat ttggttatgg agagaccgat ttctatggga atagcgatgc aaaatttttg
    11521 aaaaaagtag atcttgatac tacagatgtg aatgaactaa aaagagcaca tggaaacatg
    11581 cataatggat ttcttcaagt acttgtatca agtggtataa ttgcttttat atgtatctat
    11641 gctttctatg tgttaaatat ctttagttta ctcaaaacat tttttagaag taaagtgaat
    11701 tatgcgtttc ttggtattgt tttaattttt atactatcta tttttataga tgaaatggtg
    11761 gaggctcatg tacttttcaa taagcgcgat gtcatttcaa ttgttttctg gtattatttg
    11821 ggaagcattt cttacttgta tttaaaaaag gagaattaat atgaagacag ctttcattgc
    11881 ttggactcct ttacatttga ttaatatatt aaatgtttat caaaattttt accgagaatc
    11941 aaaaggagat ttatttattt attctgaatt tgaaaacgct agaagatata gtgagcgtat
    12001 aaaagaatta gggctattta atgatgttgt ttttgtgaat caccggaaaa tcggttcttc
    12061 ctttaaacgt aaaattaact tgttaactaa ccataatcaa tttttctcgg atgatttatc
    12121 cgtttatgat gaaattttta tacaggggga aaattatttc acaaaaatac ttttctccaa
    12181 tgtttacaaa aaaaatccag atgtaaaact aaattatata gaagatggtt tgggtactta
    12241 tttgaagaag agtatatttt caacaagtaa aaacgttcaa agagttttta aaatattaaa
    12301 taaaaatagc atcttcaaaa aaaaaattga cgattatttt gtctattgtc cggaattgtt
    12361 actttatgat gtaaaaaata taaaaaaaat tcccaaaata gatgaaaaca gtaaattgat
    12421 gaaaaacata caatcaatct ttgggataga agaagttttt gtcggagata aatgtatctt
    12481 ttttgatcaa cctttattat ctgatggtta tggcatagat gaaacagata ttttcgctaa
    12541 agtaaatagt atattttctg gaaaaaaaga aactttaatt aagcttcatc ctagagcaag
    12601 gacaaatcgt tatggtgaaa aaaacatatt gaattccagt ttaccgtttg aagtcttgct
    12661 acttgaaaat gacttctctt caacactttt gattagtcct atatctacaa tttctttttc
    12721 accatctatg atgtttgaaa agacgatcaa ctctattatg ttggctaaat taattttaaa
    12781 tgaatataca gcattatctg aagataaaaa agaaacattg acaaatattg ttcttttctg
    12841 tgaacattat aaccgtctat cagaagaaaa aattttactc ccagaaaact gggcagaatt
    12901 aagaaagttg gtagagtatg ggattattta acacactttc caagtcaaaa ttagttaagt
    12961 ccagtttctg gtatactata gggaattttt ttattaaggg aatttcattc ataactattc
    13021 ctatttttgt aaatattact actcaagcag aatatggttt gattaataat ttcacttcat
    13081 ttgtatcaat tttttcgatt cttgttgggc taagcctaaa ttcggcaatt aataatgcaa
    13141 gttttgacta taaagatgac ataaatgaat ttatgtcatc ggtactttct ctagctacac
    13201 tttcgttaat tgtgttctta ttaataggga acatatattt tttgttttca aatgcttttt
    13261 ttgatatgaa ccaacttgta tttaatttga tgttattaca aagttttgct agttttgtaa
    13321 ttagttatat ccagtcttat tttacgatta actttaacca ttttaaatat ttgattttat
    13381 cttttatatc tacttttttg aatattgcaa tctctgtagc tttgatgcag acattatatt
    13441 ttgatgataa atatgttggc agagtagtcg gcggaacact gtcatttgtg ttgataggtt
    13501 tagtaattta tttaagaata atgataaagg gtgctcattt ttttgataaa aaacagtgga
    13561 gatacgcttt aaaaatctct ttgccgctaa tcccacattc cttagcaaat atagttttag
    13621 cacaattcga tagaattatg attaattctt atttaggtcc tgctgcgggg ggaatatatt
    13681 cctacattag taacataggt attattctaa gcgtaatatg gatgtctact aataacgcct
    13741 gggttccatg gttctatggt gagatgagta aaaataatga aaaaaaagtg aagaaagtta
    13801 gtaactatta tttagtagtc tttactttga ttacgatgat tgtaatggta gtttcgattg
    13861 atttagcaaa atttatggct ccgaaagact attattctgg gttatcttta gtggttccga
    13921 taaccttagg ctatttcttt cagtttttat atagtttacc agttaatgct gaattttatg
    13981 agaaaaaaac atcgtttatt gcgattggta cagtagcaag tgctatagtt aatatactat
    14041 taaacatgat tttcatacct aggttaggaa taattgctgc tggatacaca accgtattta
    14101 cgtatttttt actttttatt tttcattata atatcgctaa aaaaattacg aataaacaat
    14161 tatttgacac aaaaaaaata tgtatcataa taatttttgt atcaattttt tctttgatac
    14221 ttcatatttt aatcaatcaa atgttgattc gttacgtttt gacatcatta ttgataatta
    14281 gtttagttat atatttttta aagattaaga atcgaattat agatgactat aattcatagg
    14341 aggagatata agtgtttaaa ggaaaaacaa tattagtaac tggcggaact ggttccattg
    14401 gaagtgaaat tgttcgtcag cttttaaaat atgaaccaaa agctatcaga atttatagta
    14461 gaggtgaaga aaaacaattt tatatgcaac aagagctctc aaatcacact aatttgagat
    14521 ttttaattgg tgatatcaga gactctcaaa ggattgatta tgcatgtcgt gatgtggata
    14581 ttatttttca tgctgccgca atgaaacacg ttccagcaag tgaatataat cctatggaag
    14641 ctgtaaaaac aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc aaaaataatg
    14701 taaaacattt tgtaggtatc agtacagata aagtagtgag tcctactaac accatgggtg
    14761 cgacaaagtt attgagtgaa aaattaataa tagctgccaa tcagtataaa ggaaaaacag
    14821 aaacaatatt ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga tctgttattc
    14881 ctctttttag taaacaaata aaaaacaata aagatattac tttgacagat gttgatatga
    14941 cacgatttat gatgtcaata ccggatgctg caagattagt tctgtctgct gctatgaatt
    15001 caaaatttgg agaaacatat gtcttaaaaa tgccggcaat caatatttta actttagctc
    15061 aatcattaat aaaaatatat aatgatacac atgatgaaaa atatagaggt gaaataaaag
    15121 ttgtaggtat caggccagga gaaaaattat atgaagagct aatgactaca gacgaaattg
    15181 aacgctcata tgaaaatgat aaaatgtata tcattccacc acactatcat gctactgacg
    15241 aactgtacaa agattatgaa aaaattaaaa tcactgcata ctcttcagat gcaacagaat
    15301 taatgagtgg acaggaattt attagaatcg caaaagagaa taatgtcgtg acttggtaag
    15361 gagttttatg atgaaatcag aagtatatgt aattgcagaa attggtgtaa accataatgg
    15421 aagtctagat ttagctttag aaagtattga taaagctaaa gaatgtggag ctaacgcggt
    15481 taaatttcaa acatttaaaa cgaataagct agtttccaaa aaggctccaa tggcaaaata
    15541 tcagaaggat aatcttaaag aggacagttc tcaatttgaa atgctaaaaa aattagaatt
    15601 atctcaatct gattttatta gaataaaaga ttattgtata caaaataaaa ttgatttttt
    15661 gtctactcct tttgatgaag aaagtgcaga ctttctaaaa caaattggtg tacaaggatt
    15721 taaaattggc tcaggagatt taacaaattt accttttctt agaaaaatag atatgtatgg
    15781 gttgccagtt ttattatcta caggtatggc tgatattcat gaggtcagtg aggctgtaga
    15841 ttgtttcata gatagtccag ttactatttt acattgtact tcaaattatc cggctagttc
    15901 tgaagatatt aatttattgg cgattaacac attaaaagaa atgttcggga tacccgttgg
    15961 gtactccgat cattcattgg gatatgatgt tggtatttgt gctgttgcga tgggtgctaa
    16021 ggtaattgag aaacatttta ccttggataa ggaactacct ggtccagatc ataaagcttc
    16081 tttagatcct caggaatttg cagattttgt aaagcatatt agaaacactg aaatattttt
    16141 aggtgatggt gttaagcgag caatgccttc tgaacttagt acaaaagaag tatcaagaaa
    16201 aagcctagtg gtcactgaga atttaaatgt gggagatatt cttacagaga aatctcttac
    16261 aataaaacgt cctggaaatg gaatagctcc taagttttta tatcaatata ttggcaaaaa
    16321 agttaaaaga ccaataagtg cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa
    16381 atacttatag taagtggaac acgagctgat tatggaatat ataaacctgt tgctgagaat
    16441 ttactacaag ataattggga tgtatcattt gttgtttctg gaatgcattt atcccataaa
    16501 tatggattta ctaaagaatt gatcttagct gacggattta gaattagtgg agaagttcaa
    16561 agtttgtttt tagagaacaa taagggaaac atggctcggt ctgtggcatt agagataaat
    16621 ggtttcacaa atatatttga acaaactagc cctgattttg tattattact aggggataga
    16681 ggagaaatgt tggcagctgc gatagcttgt ttgtatcttg gaatcaaaac agcacatatt
    16741 cacggaggcg aagtgtctgg tactgtagat gaatcaataa gacatgccat aacgaaaata
    16801 gcatctgttc attttgctgc tacggaaaaa agcaaagagc gtttaataaa aatgggggaa
    16861 gattcttgga gagtttttcc cgtcggagct cctagaatag atactatttt aaataataga
    16921 ttacctagtt ttagagatat aaaagaaaaa tatcatctaa aatttgaaaa gaaaaaatac
    16981 gttttgcaag tatttcatcc agttgtaacc gaactagaaa atatagaaaa acaaatttta
    17041 gctttgatca attcaatgga acaacaatct caaccaatta tttgcatttt acctaatagt
    17101 gatgctggta gcgaaattat cagaaaaatg taccagaaat atttaaaaac ggatattatt
    17161 tatattacga atttagagcc ggaggaatac ctgactatat taaaagagtg caaatttatg
    17221 atcggaaatt cttcttctgg gataattgaa gctgcatcat tcaaaatacc agtaataaat
    17281 ttaggaacca gacaaaatgg acgagaacaa tctaaaaata ttataaatgc aaatctaaat
    17341 gaaaatgaaa ttgaaactgc tttgaataaa atacaagatc ctcagtttat aaaaagcttg
    17401 aaaaatgtta agaacatcta cggtgacggc aacagttcta gaagaataac aaaaacattg
    17461 aatcaaatat tgaatgatgg ggaagatatt aagtggattc aaaaacaaat agcttattaa
    17521 aatatactag tgatccatac attattattg gggctggcgg acacgcaaaa gtcattattg
    17581 aaaccttaag attagaaaat aagttaatat atggattttt tgatgataag caaaataaaa
    17641 tgcaagatct gaaatatctt gggaaaataa acgaattgaa tagctgggcg gatagcaaaa
    17701 aaaaaatcta taaatttatt gtagctattg gtgactgtgt agtaagaaaa aagattataa
    17761 aaaaattgaa tatgccaagt gagcaatatg gaatagctgt tcatccaagt gctatagtgg
    17821 caaaagaaat ttcaattggt tatggaactg ttatttttgc gaatgctgta attaacccag
    17881 atgctgtaat tggagagcat gctataataa acacaggtag cattgttgag catgattgta
    17941 gaattaataa ctatgttcat ttatctccag gtgtatgctt agcgggtgga gtacatgtag
    18001 gtgtaggaac tcaaatagga attggttctc aatgtatcca gatgaagaaa attggatctt
    18061 ggtgtatgat aggcgcaggg agcactattg ttaaggatat tccctcacat tccttagctt
    18121 atggcaatcc agctaaaatt aagaaagaag gaattaattt tgaattctag aattttatta
    18181 tcaagtcctc atatgggagg aacagaagaa aaatatgttg ctaaggcttt taaagataat
    18241 tggatagcgc cattaggtga aaatgtggat aaatttgaag aaagtattac aaaatatact
    18301 ggttctaagg gcggagcttc tgcgttaagt tcaggtacag ctgccataca tcttgcctta
    18361 agactgctta atgttggaaa aaacgatatt gtctttgctc cttcatttac ttttatagct
    18421 acagtaaatc ctattttata tcaaggggct gtaccagtgt tcattgactc agaacctaat
    18481 acatggaata tgagccctgt tgcactagag agcgcattag aagaagctga aagaaacaac
    18541 caattaccta aggcaatcat cgtagtgaat ttatatggtc aaatggctga ttatgaacga
    18601 attttagcta ttgcaaacca atttaatgtt ccagtgatag aagatgcagc ggaatcgtta
    18661 ggttctactt ataagggaat ttcgtcaggc ttatttggga caattgggat ctattcattt
    18721 aacggtaata agatcatcac aacctcaggt ggaggagctc tagtgtctag tgataaaaaa
    18781 tatgttgagc attcaagatt tttagcaaca caagcaagag atcaagcacc gtattatcaa
    18841 catagcgaaa taggttataa ttaccgtcta agcaatattt ctgccggtat cggacgtgga
    18901 caaatggaag tattgaatga tagagtagct aaacgaagag aaattttcaa tcgatataag
    18961 gaagcttttt ctggaattga tagaattgaa atgatgccag aagtggatgg catggtgcca
    19021 aatcgttggt tgtctacttt aacgatcaat gattataatt ctattgatca agtagttaac
    19081 gtaataaata aactaaatga aaaaaacatt gaaacaagag ctttgtggaa accactacat
    19141 ttgcaaccag tatttcaagg ggtaaaaatg tattaccatg ataataatct tagtgtgtca
    19201 gaaaagttat ttaatagcgg cttatgctta ccttctggaa gtaatatgac agatagtgaa
    19261 caagaatttg ttattgatga gtttaaaaaa atattcaagt aggtgtgata ttttatgaac
    19321 ataggaatta ttggtttagg ttccatgggg aaaagaagac ttagactcat tcaagaacac
    19381 tttaatgata ttttactatg tggtgttgat actaatgagg cgagacgaaa ggaagttgaa
    19441 aaggaatttg gaataccaac ttataatagt ctaacagagg caaaaaattt gtttgaaata
    19501 aattcagttt ttgtttgtac atcccctata acccatgaat ttattataaa agaggcttta
    19561 aatttagatt gtaatatctt tacggaaatc aatttattaa atgaatatta tgatgaaata
    19621 atcgaagtag ctaaaaagaa agatagatta ctttatctat cttctacatt tttgaagaga
    19681 aaagaaattc aatatattaa gtctaaagtg acgcataata aaaaaatttc ttatcaatac
    19741 catgtaggac agtatttacc cgactggcat ccttgggaag attatcgcaa tttttttgtt
    19801 tcaaacaaaa aaactaatgg gtgtagagaa atattcgcta ttgaattacc ttggattata
    19861 gatacatttg ggaaagttaa atcgtttact gtaatgaccc agaaattatc caatcttgat
    19921 attgattatt cggacagttt cactcttatt attgagcatg aaactggtat agttggaact
    19981 ttaagtgtag atgttgtttc aagagtagct aaaagaaatt taactataat tggtgaaaag
    20041 attttaatag aatgggaagg taacccggat tctctatttt catggaatga agaaacttct
    20101 agtatgacag atattcaatt gtacgatgag gttatgcatg ataataatta ttcacatact
    20161 attattgaag atgcatattt agaggagatt aaagaatttt tcacgctttt ggaaaagaaa
    20221 atggaacccc aatatacttt tgaaaaagat aagtatatta ttaacttaat taacgacatt
    20281 gaggtaaatt aatgatgaaa gtaatttttt ttgggttagg gtcaattgga aagagacatc
    20341 taaaaaatct aataagctat ggaaaaaaga attctattaa ttttgatata acagcttata
    20401 gtagcagtgg aaaaaaagag tttgaagaaa taacgtatat tcaaaataga gacgatttgg
    20461 atacacatta tgatatagct tttattacaa atccgacttt taagcatctg gaaacactaa
    20521 atgtaattgt tgaaaaagtt gattacatat ttttagaaaa acctgctttt tctgagagta
    20581 ttgatcttac tcaatataat tatgattaca aaaatattta tgttgctgct ccactgaggt
    20641 ataaaaatat catggaagtt gttcgtaata gtattcaaag taaaaaagta tattctgtaa
    20701 gagctatttg ttctacttat cttcctgatt ggagaaaagg tactgattat agaaataatt
    20761 attctgcctt taaatctatg gggggaggag tagaattaga ctgtatacat gaattggatt
    20821 atattataaa cctatttggt ttccctaaat cagtaaaaaa acagtttggt aaatattcga
    20881 atcttgaaat taacagtaat gatttagcaa catatatatt gagatacgat gataaaatta
    20941 tagaaattca tttagattat tttggaagag ttcctacaag aaaatttcaa ttgtttactt
    21001 tagatgaatg tatcaatgtt gacttattaa ataataaaat aagttttgaa gaaaacattg
    21061 ttaaagaaat gcctgaatct gctaatgata tgtatgagaa ggagttgaaa tatttctttg
    21121 aaaacgtagt aacaggcaaa gaaaactgga atgatttata tcatgcaaac gaagtattga
    21181 aaatagctga ggagaaataa atggaaattt tatttactat ttgtggtaga gctgggtcta
    21241 aggggtttaa aaataaaaat ttgaaatatt tggcaggaaa gcccctagtc catttcgctt
    21301 tagcaatgat agatatctat caacaaaaac atcctgaaca taattgcacg acttgtttga
    21361 atacggacag taatgagtta gtagaattga cgactaagtt aaatccgtct gtgaaaattg
    21421 tagaacgtaa acctgaatta gctacagata ctgtagggaa aattgaagtg atacgtgatt
    21481 cattcttaca actagaaaga gaagataata tatttgattt aattatagat ttagatttga
    21541 cttcaccttt acgtgaagtt tctaatcttg aagaattaat agatgaacat ctaaaaaatt
    21601 tagataaaga cgtctacttt tcagttgttt ctgcaagacg taatccctat tttaatatgg
    21661 taaaaagaat tgaaaatgaa gttactatag ttaataaatc aagatacaca tctaggcaac
    21721 agacacctga agtttttgaa atgaatgcct caatgtattc ttttaaacct gaatttctaa
    21781 aaaaaagtaa ttatattttt gatgggcaat gtgggataat aaaaatgaga gattatctaa
    21841 ttttagatat tgatagtgaa gaagatttta aatggttaga atttttgttt cctaaatttt
    21901 tagaagattc ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg
    21961 agctgttatc ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta
    22021 ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt
    22081 gatatgacga aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa
    22141 gagatcatga aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg
    22201 gcagattcag ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg
    22261 attttagaat tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca
    22321 gcggctgttt atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca
    22381 ttgacgcctt atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat
    22441 ttatatgatg tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat
    22501 ccgaattctc catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc
    22561 gtaggagaag caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt
    22621 tatatagatg atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg
    22681 aaacaattca atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc
    22741 aatgaaattc tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc
    22801 catgactctc tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt
    22861 attttaagtg gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa
    22921 gattagaggg taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa
    22981 tctgggatag aa
//
LOCUS       DACCSK010000040        22750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCSK010000040 DACCSK010000000
VERSION     DACCSK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.040"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IVP39_001320"
     CDS             complement(218..1015)
                     /locus_tag="IVP39_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7586400.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IVP39_001321"
     CDS             complement(1012..2196)
                     /locus_tag="IVP39_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7586401.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IVP39_001322"
     CDS             complement(2221..3468)
                     /locus_tag="IVP39_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7586402.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IVP39_001323"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IVP39_001323"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7586403.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IVP39_001324"
     CDS             5448..5762
                     /locus_tag="IVP39_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7586404.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IVP39_001325"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IVP39_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7586405.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IVP39_001326"
     CDS             6447..7091
                     /locus_tag="IVP39_001326"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7586406.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IVP39_001327"
     CDS             7106..7435
                     /locus_tag="IVP39_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586407.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IVP39_001328"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IVP39_001328"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7586408.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IVP39_001329"
     CDS             8423..9247
                     /locus_tag="IVP39_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7586409.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IVP39_001330"
     CDS             9240..9587
                     /locus_tag="IVP39_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7586410.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IVP39_001331"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IVP39_001331"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7586411.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IVP39_001332"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IVP39_001332"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7586412.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVP39_001333"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVP39_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7586413.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVP39_001334"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVP39_001334"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7586414.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IVP39_001335"
     CDS             15244..16011
                     /locus_tag="IVP39_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586415.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IVP39_001336"
     CDS             16001..18043
                     /locus_tag="IVP39_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586416.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IVP39_001337"
     CDS             18324..18473
                     /locus_tag="IVP39_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7586417.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IVP39_001338"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IVP39_001338"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7586418.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IVP39_001339"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IVP39_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7586419.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IVP39_001340"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IVP39_001340"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7586420.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IVP39_001341"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IVP39_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7586421.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
BASE COUNT         7127 a         4095 c         4701 g         6827 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa
//
LOCUS       DACCSK010000041        22636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCSK010000041 DACCSK010000000
VERSION     DACCSK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.041"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..1029)
                     /locus_tag="IVP39_001342"
     CDS             complement(<1..1029)
                     /locus_tag="IVP39_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7586422.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1111..1800)
                     /locus_tag="IVP39_001343"
     CDS             complement(1111..1800)
                     /locus_tag="IVP39_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7586423.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="IVP39_001344"
     CDS             complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="IVP39_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7586424.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2327..2830)
                     /locus_tag="IVP39_001345"
     CDS             complement(2327..2830)
                     /locus_tag="IVP39_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7586425.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDAVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="IVP39_001346"
     CDS             complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="IVP39_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7586426.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="IVP39_001347"
     CDS             complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="IVP39_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7586427.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4798..5466
                     /gene="sdaAB"
                     /locus_tag="IVP39_001348"
     CDS             4798..5466
                     /gene="sdaAB"
                     /locus_tag="IVP39_001348"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7586428.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5479..6351
                     /gene="sdaAA"
                     /locus_tag="IVP39_001349"
     CDS             5479..6351
                     /gene="sdaAA"
                     /locus_tag="IVP39_001349"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7586429.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6442..7461)
                     /locus_tag="IVP39_001350"
     CDS             complement(6442..7461)
                     /locus_tag="IVP39_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7586430.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7634..8521)
                     /locus_tag="IVP39_001351"
     CDS             complement(7634..8521)
                     /locus_tag="IVP39_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7586431.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IVP39_001352"
     CDS             complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IVP39_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7586432.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IVP39_001353"
     CDS             complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IVP39_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7586433.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IVP39_001354"
     CDS             complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IVP39_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7586434.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9752..10396
                     /locus_tag="IVP39_001355"
     CDS             9752..10396
                     /locus_tag="IVP39_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7586435.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10452..10955)
                     /locus_tag="IVP39_001356"
     CDS             complement(10452..10955)
                     /locus_tag="IVP39_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7586436.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10968..11014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11552..12517
                     /locus_tag="IVP39_001357"
     CDS             11552..12517
                     /locus_tag="IVP39_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7586437.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12616..13788)
                     /locus_tag="IVP39_001358"
     CDS             complement(12616..13788)
                     /locus_tag="IVP39_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586438.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13775..14920)
                     /locus_tag="IVP39_001359"
     CDS             complement(13775..14920)
                     /locus_tag="IVP39_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586439.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14934..15866)
                     /locus_tag="IVP39_001360"
     CDS             complement(14934..15866)
                     /locus_tag="IVP39_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586440.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15983..17062
                     /locus_tag="IVP39_001361"
     CDS             15983..17062
                     /locus_tag="IVP39_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7586441.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17059..17688
                     /locus_tag="IVP39_001362"
     CDS             17059..17688
                     /locus_tag="IVP39_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586442.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17798..18025)
                     /locus_tag="IVP39_001363"
     CDS             complement(17798..18025)
                     /locus_tag="IVP39_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7586443.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18128..20716)
                     /locus_tag="IVP39_001364"
     CDS             complement(18128..20716)
                     /locus_tag="IVP39_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7586444.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20736..21395)
                     /locus_tag="IVP39_001365"
     CDS             complement(20736..21395)
                     /locus_tag="IVP39_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7586445.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21444..21947)
                     /gene="trmL"
                     /locus_tag="IVP39_001366"
     CDS             complement(21444..21947)
                     /gene="trmL"
                     /locus_tag="IVP39_001366"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7586446.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22168..>22636)
                     /locus_tag="IVP39_001367"
     CDS             complement(22168..>22636)
                     /locus_tag="IVP39_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HAP7586447.1"
                     /translation="EQSAEAFWCVADLTNLPFADQTFNAVLNIFSPSHYQEFQRVLSE
                     DGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFAEAMKLTERQRVTYEFEI
                     PEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELLKGTKRKRFSSSF"
BASE COUNT         6633 a         4766 c         4050 g         7187 t
ORIGIN      
        1 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
       61 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
      121 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
      181 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
      241 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
      301 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
      361 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
      421 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
      481 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
      541 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
      601 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
      661 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
      721 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctac
      781 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
      841 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
      901 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
      961 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt
     1021 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
     1081 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
     1141 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
     1201 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
     1261 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
     1321 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
     1381 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
     1441 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
     1501 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
     1561 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
     1621 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
     1681 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
     1741 taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt tctttttcat
     1801 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
     1861 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
     1921 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
     1981 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
     2041 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
     2101 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
     2161 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
     2221 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
     2281 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
     2341 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
     2401 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
     2461 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
     2521 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tgcatcttcg ttactgaatg
     2581 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
     2641 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
     2701 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
     2761 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
     2821 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
     2881 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
     2941 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
     3001 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
     3061 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
     3121 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
     3181 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
     3241 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
     3301 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
     3361 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
     3421 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
     3481 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
     3541 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
     3601 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
     3661 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
     3721 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
     3781 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
     3841 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
     3901 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
     3961 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
     4021 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
     4081 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
     4141 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
     4201 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
     4261 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
     4321 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
     4381 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
     4441 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
     4501 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
     4561 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
     4621 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
     4681 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
     4741 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
     4801 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
     4861 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
     4921 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
     4981 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
     5041 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
     5101 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
     5161 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
     5221 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
     5281 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
     5341 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
     5401 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
     5461 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
     5521 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
     5581 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
     5641 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
     5701 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
     5761 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
     5821 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
     5881 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
     5941 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
     6001 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
     6061 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
     6121 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
     6181 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
     6241 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
     6301 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
     6361 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
     6421 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
     6481 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
     6541 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
     6601 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
     6661 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
     6721 cgcaggacct ccatctaatt gacgtccacc atgattggat acccagatac catctgcccc
     6781 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
     6841 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
     6901 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
     6961 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
     7021 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
     7081 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
     7141 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
     7201 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
     7261 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
     7321 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
     7381 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
     7441 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
     7501 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
     7561 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
     7621 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
     7681 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
     7741 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
     7801 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
     7861 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
     7921 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
     7981 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
     8041 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
     8101 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
     8161 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
     8221 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
     8281 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
     8341 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
     8401 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
     8461 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
     8521 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
     8581 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
     8641 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
     8701 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
     8761 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
     8821 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
     8881 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
     8941 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
     9001 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
     9061 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
     9121 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
     9181 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
     9241 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
     9301 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
     9361 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
     9421 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
     9481 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
     9541 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
     9601 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
     9661 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
     9721 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
     9781 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
     9841 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
     9901 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
     9961 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    10021 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    10081 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    10141 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    10201 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    10261 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    10321 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    10381 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    10441 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    10501 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    10561 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    10621 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    10681 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    10741 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    10801 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    10861 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    10921 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    10981 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    11041 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    11101 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    11161 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    11221 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    11281 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    11341 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    11401 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    11461 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat
    11521 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
    11581 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
    11641 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
    11701 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
    11761 cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga
    11821 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaaa
    11881 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
    11941 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
    12001 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
    12061 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
    12121 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
    12181 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
    12241 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
    12301 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
    12361 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
    12421 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
    12481 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
    12541 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
    12601 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
    12661 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
    12721 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
    12781 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
    12841 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
    12901 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
    12961 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
    13021 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
    13081 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
    13141 gtttcgttta ctgatctctt tgcgattaaa cgctagattg atgacgctga aaccgctaaa
    13201 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
    13261 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
    13321 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
    13381 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
    13441 tatcttgatc gtcgccatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
    13501 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
    13561 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
    13621 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
    13681 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
    13741 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
    13801 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
    13861 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
    13921 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
    13981 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
    14041 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
    14101 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
    14161 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
    14221 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
    14281 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
    14341 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
    14401 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
    14461 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
    14521 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
    14581 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
    14641 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
    14701 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
    14761 ttccagtatt aatcgtttca agattgccac cttgatcaat ctcgccaaag gccgccataa
    14821 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
    14881 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
    14941 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
    15001 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
    15061 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
    15121 attgttcgct gaatcgctga caacacattt tctgggagta atgggatgtc gatcgtcaaa
    15181 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
    15241 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
    15301 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
    15361 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
    15421 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
    15481 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
    15541 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
    15601 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
    15661 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
    15721 agcatacagt tgatggcagt cgatttccca ctgccgtttg gacctaacag acctaagact
    15781 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
    15841 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
    15901 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
    15961 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
    16021 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
    16081 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
    16141 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
    16201 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
    16261 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
    16321 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
    16381 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
    16441 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
    16501 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
    16561 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
    16621 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
    16681 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
    16741 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
    16801 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
    16861 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
    16921 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
    16981 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
    17041 accaagggag ggacaccaat gataacagta gtcattattg atgatgatcc atttgtgacg
    17101 aaatcgttac aaaccatttt agaaagcacg aaagagatcc gtgtgttagg cattggtcat
    17161 tgtgccaagg atgcgctagt cttatacgaa acccatcgtc ccgatgtttt gctgacggac
    17221 atccgcatgc ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc
    17281 aaagccatcc ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt
    17341 tccatcggtg tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc
    17401 aaatccgctt ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa
    17461 atgatgaaga ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag
    17521 cttgcaatca tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg
    17581 tacttgagtg acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta
    17641 cgggatcgca cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac
    17701 acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc
    17761 aattgtcccg caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag
    17821 gattcgttgg attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt
    17881 ctagttttga ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt
    17941 ctttttccat ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta
    18001 atttatcttc ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat
    18061 atcacactga aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc
    18121 tttaaaatca agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa
    18181 tcatcggatc tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa
    18241 cagcaggttc tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt
    18301 ccactgtttc cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta
    18361 gcttttcttt tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc
    18421 cgaccaaaga atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat
    18481 tatccatgat ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag
    18541 tgacataggc ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg
    18601 tgtagagcaa attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca
    18661 tcttgaattc actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat
    18721 tccattcgtt tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca
    18781 ctttatcttc agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga
    18841 atacattcaa ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg
    18901 tgtcgttcca atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca
    18961 ttttatttaa cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat
    19021 cttgtgcacg gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa
    19081 tttgataagc atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg
    19141 gcagacgtcc ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc
    19201 gataaatctg attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta
    19261 cttgaccagg accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat
    19321 tcgtcggaat cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat
    19381 caacaatcaa aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc
    19441 cattcagccc cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc
    19501 gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt
    19561 ttgaaggatc caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca
    19621 cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt
    19681 cagcgatagc ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc
    19741 gaatattctt ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc
    19801 gctggatcga attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag
    19861 tctcttcttg ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat
    19921 caggctggat ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg
    19981 tctccgccgg tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga
    20041 tagctgctcg aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat
    20101 ctgcacgttt gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga
    20161 tgatatccaa agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga
    20221 acccgtaacg attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct
    20281 cagcaagcat ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat
    20341 cagcaatgat tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt
    20401 tcttcccgat tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg
    20461 ttggcttttc ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca
    20521 ctaattctcc ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga
    20581 ctacaatctc tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt
    20641 tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca
    20701 tttcttctgg ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt
    20761 tcagttccta aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa
    20821 ggctcttcag tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac
    20881 tcacttagct cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct
    20941 acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata
    21001 cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa
    21061 tgattacgca aattcggcag attatccgga tatttctggt acatttcatc gatcgactga
    21121 ccttctaaag tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga
    21181 cgagcaagct ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa
    21241 taaattgctt taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa
    21301 ctttgtggca aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat
    21361 tgtgtcttcc catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt
    21421 tattttactc tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat
    21481 tctaaattag ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc
    21541 gataaattca atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg
    21601 ttttcacgca taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc
    21661 tcttctccat cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa
    21721 tgtagtttac gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata
    21781 tttacatcat tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa
    21841 cctagtggct cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt
    21901 ccagtgtttg cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct
    21961 ctcatattta tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta
    22021 tcaactcggt gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt
    22081 atcagaacag gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc
    22141 tcaacttttt tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt
    22201 tccttttaat aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc
    22261 tttgattcgc tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt
    22321 ttcctctggt atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc
    22381 agcaaacttg gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata
    22441 aaaagcctga cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc
    22501 atcttctgag agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac
    22561 cgcgttgaat gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc
    22621 ttcggcagat tgttct
//
LOCUS       DACCSK010000042        22038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCSK010000042 DACCSK010000000
VERSION     DACCSK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.042"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(97..474)
                     /locus_tag="IVP39_001368"
     CDS             complement(97..474)
                     /locus_tag="IVP39_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586448.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            730..1458
                     /locus_tag="IVP39_001369"
     CDS             730..1458
                     /locus_tag="IVP39_001369"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7586449.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1458..1712
                     /gene="purS"
                     /locus_tag="IVP39_001370"
     CDS             1458..1712
                     /gene="purS"
                     /locus_tag="IVP39_001370"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7586450.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1714..2385
                     /gene="purQ"
                     /locus_tag="IVP39_001371"
     CDS             1714..2385
                     /gene="purQ"
                     /locus_tag="IVP39_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7586451.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2386..4608
                     /gene="purL"
                     /locus_tag="IVP39_001372"
     CDS             2386..4608
                     /gene="purL"
                     /locus_tag="IVP39_001372"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7586452.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4593..6032
                     /locus_tag="IVP39_001373"
     CDS             4593..6032
                     /locus_tag="IVP39_001373"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7586453.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6064..7107
                     /locus_tag="IVP39_001374"
     CDS             6064..7107
                     /locus_tag="IVP39_001374"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7586454.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7104..7682
                     /gene="purN"
                     /locus_tag="IVP39_001375"
     CDS             7104..7682
                     /gene="purN"
                     /locus_tag="IVP39_001375"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7586455.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7679..9214
                     /gene="purH"
                     /locus_tag="IVP39_001376"
     CDS             7679..9214
                     /gene="purH"
                     /locus_tag="IVP39_001376"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7586456.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9230..10504
                     /gene="purD"
                     /locus_tag="IVP39_001377"
     CDS             9230..10504
                     /gene="purD"
                     /locus_tag="IVP39_001377"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7586457.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10626..11540
                     /locus_tag="IVP39_001378"
     CDS             10626..11540
                     /locus_tag="IVP39_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7586458.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11673..13238)
                     /locus_tag="IVP39_001379"
     CDS             complement(11673..13238)
                     /locus_tag="IVP39_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7586459.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13231..14682)
                     /locus_tag="IVP39_001380"
     CDS             complement(13231..14682)
                     /locus_tag="IVP39_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7586460.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14696..15712)
                     /locus_tag="IVP39_001381"
     CDS             complement(14696..15712)
                     /locus_tag="IVP39_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7586461.1"
                     /translation="MKRVLFFLALVVTAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15932..17020
                     /locus_tag="IVP39_001382"
     CDS             15932..17020
                     /locus_tag="IVP39_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7586462.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17074..18600
                     /locus_tag="IVP39_001383"
     CDS             17074..18600
                     /locus_tag="IVP39_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7586463.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18602..19807
                     /locus_tag="IVP39_001384"
     CDS             18602..19807
                     /locus_tag="IVP39_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7586464.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20064..21041
                     /gene="guaC"
                     /locus_tag="IVP39_001385"
     CDS             20064..21041
                     /gene="guaC"
                     /locus_tag="IVP39_001385"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7586465.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21305..>22038
                     /locus_tag="IVP39_001386"
     CDS             21305..>22038
                     /locus_tag="IVP39_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7586466.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKES"
BASE COUNT         7069 a         3724 c         5005 g         6240 t
ORIGIN      
        1 ttttagttta ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc
       61 tttaccgtgc ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt
      121 ataactaaaa tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat
      181 ctcttgtcca ttgaataaga tctggatacg gtaatgattt ttgaaacgct tctgttcatg
      241 aatctgactg attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg
      301 gcattcagca cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc
      361 tttattgcat aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc
      421 ttcagctgtt aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa
      481 gtgcctcctc gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta
      541 taaatgtaaa cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa
      601 aagaatctaa tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa
      661 atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga
      721 gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt
      781 acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc
      841 gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt
      901 ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc
      961 gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca
     1021 gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc
     1081 attgaattct actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc
     1141 tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca agcgttggct
     1201 attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag
     1261 ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact
     1321 tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat
     1381 ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa
     1441 gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt
     1501 tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg
     1561 aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg
     1621 atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc
     1681 gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca
     1741 ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc
     1801 gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga
     1861 ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg
     1921 gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc
     1981 cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat
     2041 tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat
     2101 gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac
     2161 gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac
     2221 atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga
     2281 atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg
     2341 ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa
     2401 gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat
     2461 gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact
     2521 ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta
     2581 cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc
     2641 gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca
     2701 gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc
     2761 agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat
     2821 ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt
     2881 atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca
     2941 ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc
     3001 gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac
     3061 ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta
     3121 caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg
     3181 catcattcgg attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct
     3241 agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca
     3301 caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg
     3361 ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat
     3421 ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa
     3481 ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat
     3541 aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca
     3601 gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca
     3661 aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca
     3721 ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca
     3781 gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca
     3841 gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg
     3901 aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga
     3961 attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat
     4021 aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt
     4081 gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata
     4141 ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga
     4201 atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta
     4261 ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg
     4321 gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa
     4381 ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg
     4441 gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg
     4501 acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca
     4561 gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg
     4621 aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg
     4681 ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag
     4741 gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa
     4801 aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca
     4861 atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct
     4921 tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg
     4981 gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg
     5041 aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg
     5101 cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc
     5161 cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat
     5221 tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca
     5281 atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga
     5341 tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg
     5401 cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg
     5461 ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc
     5521 cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc
     5581 ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag
     5641 gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg
     5701 tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt
     5761 tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa
     5821 atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg
     5881 aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta
     5941 tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg
     6001 aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat
     6061 tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta
     6121 gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga
     6181 ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg
     6241 gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca
     6301 atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg
     6361 ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta
     6421 gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca
     6481 gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg
     6541 atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt
     6601 ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt
     6661 gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa
     6721 gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag
     6781 aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg
     6841 ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc
     6901 ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg
     6961 ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt
     7021 gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt
     7081 gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca
     7141 actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt
     7201 tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg
     7261 cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc
     7321 ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg
     7381 gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac
     7441 ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg
     7501 gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa
     7561 aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag
     7621 agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat
     7681 gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt
     7741 gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc
     7801 tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg
     7861 tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag
     7921 ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt
     7981 atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa
     8041 cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac
     8101 ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca
     8161 tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata
     8221 tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt
     8281 gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc
     8341 tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca
     8401 tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa
     8461 ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac
     8521 agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt
     8581 tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa
     8641 gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca
     8701 aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg
     8761 agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga
     8821 aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc
     8881 attagcgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa
     8941 cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat
     9001 tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt
     9061 tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc
     9121 aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat
     9181 ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt
     9241 ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa
     9301 ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg
     9361 tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat
     9421 tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat
     9481 ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa
     9541 aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt
     9601 tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa
     9661 agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct
     9721 ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt
     9781 cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa
     9841 ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc
     9901 aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga
     9961 aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc
    10021 tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga
    10081 attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga
    10141 ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca
    10201 agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac
    10261 aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc
    10321 ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg
    10381 tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga
    10441 tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa
    10501 gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt
    10561 agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag
    10621 ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag
    10681 gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa
    10741 acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct
    10801 ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat
    10861 gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag
    10921 aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag
    10981 atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa
    11041 aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat
    11101 atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga
    11161 tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg
    11221 tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg
    11281 gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag
    11341 aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct tggattttac
    11401 gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa
    11461 tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg
    11521 ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa
    11581 gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa
    11641 tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc
    11701 tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata actgaaatag
    11761 ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc
    11821 aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta
    11881 aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc
    11941 caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc
    12001 aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg
    12061 aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg
    12121 gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg
    12181 agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt
    12241 ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt
    12301 gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca
    12361 tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca
    12421 aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta
    12481 tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg
    12541 atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca
    12601 gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg
    12661 taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct
    12721 gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct
    12781 ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg
    12841 tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat
    12901 tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc
    12961 atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc
    13021 cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg
    13081 atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg
    13141 acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga
    13201 atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt
    13261 gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt
    13321 gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc
    13381 gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag
    13441 tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat
    13501 tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac
    13561 agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc
    13621 tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata
    13681 atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga
    13741 cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg
    13801 ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag taatctgagc
    13861 ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa
    13921 agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag
    13981 tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc
    14041 gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc
    14101 atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg
    14161 gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa
    14221 gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc
    14281 cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc
    14341 gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt
    14401 gatctctcga atatcattcc taagttcttc taggatattt tcctcattga actcatctac
    14461 ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa
    14521 agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg
    14581 cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt
    14641 tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat
    14701 aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa
    14761 tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg
    14821 ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta
    14881 gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt
    14941 tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt
    15001 ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc
    15061 ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt
    15121 cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg
    15181 agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct
    15241 gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt
    15301 tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt
    15361 cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg
    15421 attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat
    15481 cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt
    15541 tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg
    15601 attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca
    15661 tcatttggta agcggttaca acaagggcta aaaaaaataa aacacgtttc actttaaatc
    15721 actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag
    15781 gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta
    15841 aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac
    15901 tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg
    15961 gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc
    16021 agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat
    16081 cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca
    16141 atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg
    16201 ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa
    16261 agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa
    16321 tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat
    16381 tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg
    16441 ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc
    16501 atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc
    16561 tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa
    16621 ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat
    16681 catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac
    16741 tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac
    16801 gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag
    16861 tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt
    16921 ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat
    16981 cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact
    17041 aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca
    17101 gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa
    17161 cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat
    17221 gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag
    17281 ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa
    17341 gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa
    17401 gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat
    17461 aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag
    17521 caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag
    17581 cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg
    17641 aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc
    17701 gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg
    17761 atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga tcgttatcct
    17821 gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac
    17881 cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag
    17941 attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt
    18001 ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca
    18061 gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa
    18121 tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat
    18181 aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc
    18241 cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt
    18301 gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt
    18361 ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc
    18421 gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata
    18481 ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg
    18541 cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa
    18601 gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag
    18661 agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc
    18721 gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta accttgttct
    18781 gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca
    18841 tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat
    18901 ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct
    18961 gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac
    19021 tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct
    19081 agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga
    19141 aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt
    19201 tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc
    19261 aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat
    19321 ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc
    19381 atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa
    19441 tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt
    19501 caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc
    19561 aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg
    19621 tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat
    19681 gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat
    19741 caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac
    19801 aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct
    19861 ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt
    19921 ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa
    19981 attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat
    20041 gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc
    20101 ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac
    20161 atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg
    20221 cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc
    20281 gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg
    20341 tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt
    20401 atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata
    20461 ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg
    20521 tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag
    20581 tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta
    20641 gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg
    20701 gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg
    20761 ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat
    20821 actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa
    20881 tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc
    20941 aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg
    21001 tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag
    21061 gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct
    21121 ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt
    21181 cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt
    21241 tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa
    21301 gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca
    21361 gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg
    21421 aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct
    21481 tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga
    21541 atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga
    21601 gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt
    21661 tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc
    21721 ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc
    21781 gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa
    21841 ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct
    21901 ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt
    21961 caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga
    22021 aaaactgaaa gaatcgat
//
LOCUS       DACCSK010000043        21758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCSK010000043 DACCSK010000000
VERSION     DACCSK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.043"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(135..854)
                     /locus_tag="IVP39_001387"
     CDS             complement(135..854)
                     /locus_tag="IVP39_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7586467.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            1191..1523
                     /locus_tag="IVP39_001388"
     CDS             1191..1523
                     /locus_tag="IVP39_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586468.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1510..3513
                     /locus_tag="IVP39_001389"
     CDS             1510..3513
                     /locus_tag="IVP39_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7586469.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3532..4002
                     /locus_tag="IVP39_001390"
     CDS             3532..4002
                     /locus_tag="IVP39_001390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7586470.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            4023..4607
                     /locus_tag="IVP39_001391"
     CDS             4023..4607
                     /locus_tag="IVP39_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7586471.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            4622..5608
                     /locus_tag="IVP39_001392"
     CDS             4622..5608
                     /locus_tag="IVP39_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7586472.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            5598..5909
                     /locus_tag="IVP39_001393"
     CDS             5598..5909
                     /locus_tag="IVP39_001393"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7586473.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            5931..7712
                     /locus_tag="IVP39_001394"
     CDS             5931..7712
                     /locus_tag="IVP39_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7586474.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            7705..9081
                     /locus_tag="IVP39_001395"
     CDS             7705..9081
                     /locus_tag="IVP39_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7586475.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            9088..9723
                     /locus_tag="IVP39_001396"
     CDS             9088..9723
                     /locus_tag="IVP39_001396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7586476.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            9753..11108
                     /locus_tag="IVP39_001397"
     CDS             9753..11108
                     /locus_tag="IVP39_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7586477.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(11300..12694)
                     /locus_tag="IVP39_001398"
     CDS             complement(11300..12694)
                     /locus_tag="IVP39_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7586478.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            12786..13757
                     /locus_tag="IVP39_001399"
     CDS             12786..13757
                     /locus_tag="IVP39_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7586479.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13754..14536
                     /locus_tag="IVP39_001400"
     CDS             13754..14536
                     /locus_tag="IVP39_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7586480.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(14705..15076)
                     /locus_tag="IVP39_001401"
     CDS             complement(14705..15076)
                     /locus_tag="IVP39_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7586481.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            15210..15653
                     /locus_tag="IVP39_001402"
     CDS             15210..15653
                     /locus_tag="IVP39_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7586482.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(15696..16505)
                     /gene="yidA"
                     /locus_tag="IVP39_001403"
     CDS             complement(15696..16505)
                     /gene="yidA"
                     /locus_tag="IVP39_001403"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7586483.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16524..17891)
                     /locus_tag="IVP39_001404"
     CDS             complement(16524..17891)
                     /locus_tag="IVP39_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7586484.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(17875..18705)
                     /locus_tag="IVP39_001405"
     CDS             complement(17875..18705)
                     /locus_tag="IVP39_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7586485.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPKSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            18868..19305
                     /locus_tag="IVP39_001406"
     CDS             18868..19305
                     /locus_tag="IVP39_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7586486.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            19381..20001
                     /locus_tag="IVP39_001407"
     CDS             19381..20001
                     /locus_tag="IVP39_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7586487.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            20190..20696
                     /locus_tag="IVP39_001408"
     CDS             20190..20696
                     /locus_tag="IVP39_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7586488.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            20713..21612
                     /locus_tag="IVP39_001409"
     CDS             20713..21612
                     /locus_tag="IVP39_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7586489.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
BASE COUNT         7022 a         3731 c         4684 g         6321 t
ORIGIN      
        1 gtataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
       61 aaaagccatt ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaaccc
      121 cctcagaaag tctattaaaa ttcattcaca aagaaatcga ataagcgctg ttcagatgga
      181 tgtacacggt gggtaagttc cggatgccat tgaacaccga ggattttatg catatcatct
      241 ttgctttgaa ccgcttcaac gagtccatcg ttagaaaaag cgatggcttt taataaaggt
      301 gaaagatctt tgactgcctg atgatggtaa gaattcacaa aatatggatc ggggagaagg
      361 ttgcttaaca aactgtctga aacaatcttg acttcgtgag tcgtaaattg cggggcagtg
      421 ggctgttgtt catgttttac tgaccacttt ggatataatg aaagatcttg ataaagtgta
      481 ccttccaaaa cgacattcaa tagttggagt ccccggcaga ctgcaaaaat tggttttctt
      541 tgtttcaatg cttccaagat cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc
      601 gttagctttg gatgaggatc ttccatgtaa agttgtggac tgacatcttg accacctgca
      661 agaataagtt tgtcgatttg tccaatcagt tgaggagctg aatctggaga catgactggc
      721 agaataagag gcaatccttg agcatctaaa actgcatcca cgaatccttg cggtgtatag
      781 ctcacttgat ttccttggaa agtatctgtt gctcgaatca gttgatttcc ggcaatgccg
      841 atgattggtt tcatttgtat tccccctaaa gtagacttat tgtaacaagg atttaaaaaa
      901 aattaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
      961 aagagaaatt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
     1021 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
     1081 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
     1141 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
     1201 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa
     1261 cactgcggca gcagctgaag atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc
     1321 aaacaaacta tcgacaacaa ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac
     1381 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
     1441 tgaaagatca atatcaaaag catcagagtc aagcgatcgg tgcgatcatt gaaagggtga
     1501 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
     1561 caggagagga ttttacaagc agtacaagga atccaaaacg ttgaaatccg ggatctgtat
     1621 caaaatagaa caaacaacca gtgggtggag aagtattttc cagataccac gattattgat
     1681 aaagaagcaa aattaagcgc tttaggtaat cggttgaaag acatccaaga agctatccaa
     1741 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
     1801 tccttacaag atttggaagc caattattcc gaagaaaatt tcataaataa attgaaagaa
     1861 gtccttgatt taaaagaaaa gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa
     1921 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
     1981 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
     2041 gaactgagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
     2101 tatctttcgt atgttgcttt aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa
     2161 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa actgcaagag
     2221 accaagcagc aattatccat tgttcacgag cagcaaaaga agataacgac agcgttaggt
     2281 cagtgcagtg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
     2341 gaacgagaaa aaataaaaga acggtttatc catgcgactt atctgatcgt tttgcaaggt
     2401 tgggtagatg cagaagaaaa acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa
     2461 gaagatatat acgtcagttt cgaaacacca aactcagaag agattcagca ggaagtccct
     2521 actaaattga aaaaccatcc gcttgtcgaa ccttttgaat tgctgactga aatgtacagt
     2581 ctgccaaaat atgaagaagt agatccaaca ccttggatga caccatttta tcttgtcttc
     2641 ttcgggatga tggtcgcaga tgttggttat ggtttgctga tgctcattgg gacaatacta
     2701 gctcaaaaac tacttgttct accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt
     2761 ctctcgatac catcgatcat ctggggattg atctacagtt catttttcgg acagtcatta
     2821 ccaaaagaac tgtttggcat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
     2881 aatacgattt tgattttatc cgtgattttc ggtctgatac agattttggt tggattattt
     2941 gtggcagcaa aggaaaatat caggagaaaa gattacttga gtgctgtcag tgatggattt
     3001 gcctggcaag ggatcctaat tgggatcgtt attgcgttgg ttggtgccat gttgataaaa
     3061 aatacagcat ttgtttacct tggcgctggg ctatcgatca ttgctgcctt atgtatcttg
     3121 atcgttccga ttatccaaac gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat
     3181 ctttacggac tgaccggcta tatcggtgat ttagtcagtt atacacgttt gatggcattg
     3241 ggtatttcag gcggaagtat tggagcggca ttcaatatgc tagttgcttt catgccgcca
     3301 gctgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
     3361 ttgacactgc taagtgccta tgtccatggc gcacgattgc agtatgtcga attttttggg
     3421 aagttttata caggaggcgg acgtgccttt gatccgctga aaacggcgga aaaatacgtc
     3481 aacatcaatc acaagaaaaa aaacaataaa taattggagg atttttatat aatgatggat
     3541 tacttaatta gtcaaaatgg tggaatggta tttgcggtat tagcgatggc aacagcgacg
     3601 attttttcag ggatcggttc agctaaaggt gttggaatga caggtgaggc agcagcagca
     3661 ttgacaacaa gtcaacccga aaaattcggg caggctctga ttctgcaatt gcttccaggc
     3721 acgcaaggtt tgtatggatt cgttattgcc tttttgattt ttatcaactt aggaaatgac
     3781 atgtcagttg tacaaggact gaactttcta ggtgcttctc tgccgattgc ttttacaggc
     3841 ttgttttcag gtattgccca aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa
     3901 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atacgcgatt
     3961 ttaggtttcg ttatttcctt cctattagtg ttgaatgcct aagcataaag gaggaaaagc
     4021 agatgaacgc cattgaaaaa atcatctctc agatgaacga agcagcagag caggaaagag
     4081 cggcgttaga acaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
     4141 aagttgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat
     4201 accgtcagct aaggaatcgc cagcaagtag aagtacgaca agaaaatttg aatgcgaagc
     4261 aggaattttt aagacgattg tttgcagatg cggtaactga aatggaaaat tgggatgaat
     4321 ctgaacaaat ccagtttatc aaaaatgcat tgtattcatt acctttgaca ggaaaagtag
     4381 cattcattgc tggagaaaaa tcagcagcct atctttctca gacgttgttg gatgaatgga
     4441 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
     4501 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
     4561 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
     4621 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
     4681 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
     4741 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
     4801 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
     4861 cgtttggatc tatacgatgc gttatacttt ccataatctg aaggttttga caaaagctga
     4921 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
     4981 cttgaaggat gccattcata cgcaagtttc aaatgaactt cctgaaagca ttatggacag
     5041 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
     5101 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
     5161 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
     5221 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
     5281 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
     5341 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct
     5401 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
     5461 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
     5521 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa
     5581 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
     5641 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
     5701 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
     5761 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
     5821 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
     5881 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
     5941 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
     6001 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
     6061 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
     6121 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
     6181 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
     6241 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
     6301 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
     6361 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
     6421 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
     6481 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
     6541 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
     6601 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
     6661 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
     6721 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
     6781 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
     6841 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
     6901 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
     6961 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
     7021 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
     7081 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
     7141 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
     7201 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
     7261 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
     7321 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
     7381 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
     7441 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
     7501 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
     7561 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
     7621 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
     7681 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
     7741 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
     7801 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
     7861 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
     7921 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
     7981 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
     8041 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
     8101 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
     8161 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
     8221 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
     8281 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
     8341 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
     8401 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
     8461 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
     8521 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
     8581 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
     8641 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
     8701 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
     8761 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
     8821 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
     8881 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
     8941 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
     9001 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
     9061 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
     9121 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
     9181 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
     9241 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
     9301 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
     9361 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
     9421 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
     9481 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
     9541 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
     9601 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
     9661 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
     9721 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
     9781 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
     9841 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
     9901 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
     9961 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
    10021 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
    10081 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    10141 tgctttacat cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt
    10201 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    10261 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    10321 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    10381 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
    10441 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    10501 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    10561 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    10621 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
    10681 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
    10741 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    10801 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    10861 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    10921 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    10981 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    11041 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    11101 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    11161 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    11221 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
    11281 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    11341 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    11401 gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    11461 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
    11521 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
    11581 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    11641 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    11701 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    11761 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
    11821 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    11881 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    11941 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    12001 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    12061 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    12121 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    12181 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    12241 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    12301 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    12361 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    12421 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    12481 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    12541 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    12601 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    12661 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    12721 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    12781 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
    12841 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
    12901 gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt
    12961 atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc
    13021 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
    13081 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
    13141 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
    13201 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
    13261 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
    13321 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
    13381 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
    13441 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
    13501 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
    13561 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
    13621 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
    13681 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
    13741 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
    13801 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
    13861 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
    13921 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
    13981 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
    14041 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
    14101 tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa
    14161 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
    14221 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat
    14281 cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac
    14341 aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga
    14401 aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga
    14461 atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg
    14521 tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac
    14581 cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt
    14641 gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata
    14701 ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag
    14761 aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccagca gctttatgtt
    14821 gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta
    14881 gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa
    14941 gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa
    15001 aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg
    15061 tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga
    15121 agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga
    15181 aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc
    15241 aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga
    15301 ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag
    15361 aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag
    15421 aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt
    15481 attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa
    15541 ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac
    15601 gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag
    15661 cgagtagatc agctttctct cagttttttt atttattaca gtacatattt tttgattgct
    15721 tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga
    15781 gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc
    15841 tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc
    15901 gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt
    15961 ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa
    16021 atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca
    16081 ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg
    16141 atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca
    16201 cggcgtgcca ttacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg
    16261 attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta
    16321 tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg
    16381 cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt
    16441 gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata
    16501 gacataattt cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg
    16561 ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca
    16621 accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca
    16681 acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt
    16741 tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa
    16801 ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct
    16861 tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc
    16921 aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag
    16981 taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca
    17041 agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac
    17101 tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg
    17161 aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg
    17221 ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta
    17281 agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt
    17341 cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt
    17401 tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac
    17461 acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag
    17521 atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat
    17581 aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa
    17641 tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta
    17701 aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt
    17761 cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg
    17821 atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa
    17881 ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt
    17941 gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact
    18001 tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag
    18061 gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc
    18121 atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccacg
    18181 tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg
    18241 acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca
    18301 ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa
    18361 taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac ttttggggaa
    18421 aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct
    18481 gatcactgga gcgtaacctg catcttccag tgtttggatc ccgtcactta gaaaaggagt
    18541 tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat
    18601 ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc
    18661 gtttttttga taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt
    18721 gtataaattc atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa
    18781 aggttttctg taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta
    18841 gctatgagaa gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc
    18901 aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa
    18961 gggcaaatgg tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag
    19021 acgggcgatg agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc
    19081 cgtgcaggag aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc
    19141 tatcgaacac catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt
    19201 ttgaaagacc gtcccacagc agggaaggtt cgaatcgatt atgatctgtt catgggaccg
    19261 gaaaaaatcg gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt
    19321 ttgatttttt gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga
    19381 ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca
    19441 cacgcgatct tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc
    19501 caaaactatc ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact
    19561 gacttgaata ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca
    19621 tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa
    19681 gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac
    19741 gcgatcgatt acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat
    19801 gatctgtttg atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg
    19861 caagaaatcg gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa
    19921 gaagatttaa gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt
    19981 tctgactctg aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca
    20041 gcctatcaga ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa
    20101 aatcccaagt aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc
    20161 atattaagtg aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc
    20221 agatcgaaaa ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt
    20281 tccgtatcac gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata
    20341 gttttcataa agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat
    20401 ggttttatcg gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc
    20461 ctgtggactt atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg
    20521 cagagctcca tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct
    20581 acttttttga aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca
    20641 cagttaaaac gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga
    20701 gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa
    20761 gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag
    20821 caatcattta taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg
    20881 gctatcgtaa tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca
    20941 gaagatttac ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat
    21001 cgatctgaaa aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat
    21061 aaaacaattc atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa
    21121 gctgaaaaag aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag
    21181 aaaatcgtag acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca
    21241 gatagccatg ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca
    21301 cttccacttt tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg
    21361 cagatggccg aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt
    21421 caagaaggga cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac
    21481 aaagagcatc aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt
    21541 acaccagaat ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat
    21601 ttggatcatt agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag
    21661 taaataataa gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta
    21721 tcagtattat tgctttatcc gtgcttttgt ttttcgaa
//
LOCUS       DACCSK010000044        21394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCSK010000044 DACCSK010000000
VERSION     DACCSK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.044"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IVP39_001410"
     CDS             185..1183
                     /locus_tag="IVP39_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7586490.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IVP39_001411"
     CDS             1295..1804
                     /locus_tag="IVP39_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7586491.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IVP39_001412"
     CDS             complement(1834..2493)
                     /locus_tag="IVP39_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7586492.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IVP39_001413"
     CDS             complement(2495..3259)
                     /locus_tag="IVP39_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7586493.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IVP39_001414"
     CDS             complement(3267..3944)
                     /locus_tag="IVP39_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7586494.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IVP39_001415"
     CDS             complement(3981..5366)
                     /locus_tag="IVP39_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7586495.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5335..5589)
                     /locus_tag="IVP39_001416"
     CDS             complement(5335..5589)
                     /locus_tag="IVP39_001416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586496.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IVP39_001417"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVP39_001417"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVP39_001418"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVP39_001418"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7586497.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IVP39_001419"
     CDS             6305..7162
                     /locus_tag="IVP39_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7586498.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IVP39_001420"
     CDS             complement(7243..7578)
                     /locus_tag="IVP39_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586499.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IVP39_001421"
     CDS             complement(7645..9015)
                     /locus_tag="IVP39_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7586500.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IVP39_001422"
     CDS             complement(9093..9629)
                     /locus_tag="IVP39_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7586501.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IVP39_001423"
     CDS             9938..11419
                     /locus_tag="IVP39_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7586502.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="IVP39_001424"
     CDS             complement(11522..12229)
                     /locus_tag="IVP39_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7586503.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="IVP39_001425"
     CDS             complement(12308..12646)
                     /locus_tag="IVP39_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7586504.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="IVP39_001426"
     CDS             complement(12757..13719)
                     /locus_tag="IVP39_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7586505.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="IVP39_001427"
     CDS             complement(13734..15065)
                     /locus_tag="IVP39_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7586506.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="IVP39_001428"
     CDS             complement(15073..15753)
                     /locus_tag="IVP39_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7586507.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="IVP39_001429"
     CDS             complement(15972..16451)
                     /locus_tag="IVP39_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7586508.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="IVP39_001430"
     CDS             complement(16578..18257)
                     /locus_tag="IVP39_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7586509.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="IVP39_001431"
     CDS             18805..20307
                     /locus_tag="IVP39_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586510.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="IVP39_001432"
     CDS             20320..20982
                     /locus_tag="IVP39_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586511.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="IVP39_001433"
     CDS             complement(21083..21286)
                     /locus_tag="IVP39_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7586512.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         6354 a         4435 c         3635 g         6970 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaac caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca taaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag agccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctgtcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgtt
//
LOCUS       DACCSK010000045        21226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCSK010000045 DACCSK010000000
VERSION     DACCSK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.045"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..456)
                     /locus_tag="IVP39_001434"
     CDS             complement(<1..456)
                     /locus_tag="IVP39_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7586513.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQ"
     gene            complement(474..1388)
                     /gene="pfkB"
                     /locus_tag="IVP39_001435"
     CDS             complement(474..1388)
                     /gene="pfkB"
                     /locus_tag="IVP39_001435"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7586514.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKKDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(1385..2137)
                     /locus_tag="IVP39_001436"
     CDS             complement(1385..2137)
                     /locus_tag="IVP39_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7586515.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(2297..2506)
                     /locus_tag="IVP39_001437"
     CDS             complement(2297..2506)
                     /locus_tag="IVP39_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586516.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(2517..3845)
                     /locus_tag="IVP39_001438"
     CDS             complement(2517..3845)
                     /locus_tag="IVP39_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586517.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(4210..4797)
                     /locus_tag="IVP39_001439"
     CDS             complement(4210..4797)
                     /locus_tag="IVP39_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7586518.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(4880..6130)
                     /gene="clpX"
                     /locus_tag="IVP39_001440"
     CDS             complement(4880..6130)
                     /gene="clpX"
                     /locus_tag="IVP39_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7586519.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(6377..7666)
                     /locus_tag="IVP39_001441"
     CDS             complement(6377..7666)
                     /locus_tag="IVP39_001441"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7586520.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(7820..8752)
                     /locus_tag="IVP39_001442"
     CDS             complement(7820..8752)
                     /locus_tag="IVP39_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586521.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(8909..9568)
                     /locus_tag="IVP39_001443"
     CDS             complement(8909..9568)
                     /locus_tag="IVP39_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7586522.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(9571..10596)
                     /gene="sppA"
                     /locus_tag="IVP39_001444"
     CDS             complement(9571..10596)
                     /gene="sppA"
                     /locus_tag="IVP39_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7586523.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(10609..10899)
                     /locus_tag="IVP39_001445"
     CDS             complement(10609..10899)
                     /locus_tag="IVP39_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586524.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            11308..13113
                     /gene="glmS"
                     /locus_tag="IVP39_001446"
     CDS             11308..13113
                     /gene="glmS"
                     /locus_tag="IVP39_001446"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7586525.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(13185..14540)
                     /locus_tag="IVP39_001447"
     CDS             complement(13185..14540)
                     /locus_tag="IVP39_001447"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7586526.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(14564..15733)
                     /locus_tag="IVP39_001448"
     CDS             complement(14564..15733)
                     /locus_tag="IVP39_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586527.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(15730..16617)
                     /locus_tag="IVP39_001449"
     CDS             complement(15730..16617)
                     /locus_tag="IVP39_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7586528.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(16806..20498)
                     /gene="nifJ"
                     /locus_tag="IVP39_001450"
     CDS             complement(16806..20498)
                     /gene="nifJ"
                     /locus_tag="IVP39_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7586529.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLNLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            20697..21218
                     /locus_tag="IVP39_001451"
     CDS             20697..21218
                     /locus_tag="IVP39_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7586530.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         5893 a         4565 c         3769 g         6999 t
ORIGIN      
        1 ttgctcttca gctgtttgga acaagtcttg gacttcttct ggtgtatttg cttcttttaa
       61 ttttcctacg aaatctgcat caatcagcaa tcgagatagt gctgctaatg cttgcaaatg
      121 tgtgtcgttt gcgccttctg gagctgcgat catgaaaaac agataagttg gctgtccgtc
      181 taaggcttca taatctactc ctttttggct ttttgcgaat aaaactgttg cttctttaac
      241 tgcactgttt ttcgcgtgag gcatggcgat tccgtcacca agacctgtag aagtttgtgc
      301 ttcgcgggct agaattcctt ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa
      361 cattttttgg accatttcat cgattgcgcc ttttttatca gtcgcttgca aatccatgat
      421 catcgcttct ttgaccatta aatccttgat attcatgatt atcctccttg aaactaatca
      481 atttttgtga tttcaacttc tggcagcaac tcatcaatca attcgcgagc tgccaagtca
      541 tcagaaaaag ctgttgcgct tccgcaagca actccccatt taaaggcttc gattggatct
      601 ttcgtacggc tgtaagcccc aataaagcca gcaatcattg aatcccctgc acctactgaa
      661 tttttcaatg ggcgaactaa tacattcgtt ttataagttc cttgttccgt gaagagcaaa
      721 gcaccgtctc cagccattga gatcagcaca ttctgtgccc cttcagctag taattttttc
      781 ccatacggaa tgatttcttc tacagattgg aaggttgtgt tgtaaagctc agctaattcg
      841 tgattgtttg gtttgatcaa caacggtttc ttaacaagtg cattttttag gtcatcacct
      901 gttgtatcaa tcacgaaatc tgcacctttt tctcgaatga tatcaatcag ttcttgataa
      961 aatccttttc gcaggctagc tggcgcgctt cctgacaata cgacgatatc tccttgtcct
     1021 actgttccta atgcttgctt caactcatcg atttcttcat ctgaaataga tgggcctaag
     1081 ccattgatct cagtttcagt gtctgatttt agtttgatat tgattcttgt atcttttttt
     1141 acggttgtga acaaagactg gatttgttct ttttcaagcc acttcgaaat aaattcgcca
     1201 gtgaaccctc ctaaaaaccc tagtgctgta gattgagaat cgatccgttt caaaatacgg
     1261 gaaacattga tcccttttcc tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg
     1321 cctagtttta acccatctac gcgtacgata taatcaattg atggattaag tgttactgtg
     1381 taaatcattg tattgcctcc ttaattgttg tcttttcacg gaattcatta acgaattcta
     1441 atggacaacg ctcggttatg atcgtggctg cttccaaagg tgcaacagag gtgaaggaca
     1501 actggttgaa tttggtatga tcaatcaata caaatgtctc ttcggaattt ttcatggcta
     1561 aacgtttaac agctgcctct tctggatccg gagtcgtgaa tccttgttca agatgggcac
     1621 cgttcattcc cataaatgct ttattaaaat gaaaattttt tagttgttct atcgcattag
     1681 atcctaaaac agcattagtt gccagcttta tcgttccacc taaaataatg gtagcaagct
     1741 ccaaatcaac taaatttgcc gcatgttgaa cggaatttgt cactactgtg atctgttttc
     1801 cttgcagaaa aggaatcatt tctaacgtag tagagccagc gtctaaataa atgacatctt
     1861 cgtcattaat ggtttcagct gccgatttgg cgatttgctg tttttcgtga acgtttttga
     1921 gtgatttttc actcatattc tgctcaaaat tcaataattg ctctttctta gctccgccat
     1981 gaactctttc tagaaaccca ctattctcca gctcttgtaa atcacgtctt atcgttgatt
     2041 cagaagcatt caaaaggttt acaagatctt gtgatttcac gattttctgc tgttcaagca
     2101 agtctaggat tttttgtctg cgttcctctg taagcatttt cacgatcacc tcaatacaaa
     2161 taatagcata ttttttttct actagcaata taaatcagtc aaaaacgttc ataaaaatta
     2221 taaatcagtc aaaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt
     2281 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     2341 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     2401 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     2461 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     2521 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     2581 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     2641 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     2701 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     2761 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     2821 gtcggatcga taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa
     2881 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata
     2941 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     3001 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     3061 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     3121 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     3181 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     3241 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     3301 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     3361 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     3421 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     3481 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     3541 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     3601 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     3661 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     3721 cggataaatg ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt
     3781 tttttttctt cttcatttaa gtctgtgagc tggatagtca tcaataattt atcttcgcca
     3841 atcattttct cacctctttc aaatcttttt ataatttaat tataaccaat gataaagaaa
     3901 aactaaaact ttatgtctta gaaaccaatt tggacgatta catgatagac taagtaaaaa
     3961 gaaacaaaga ggtgtttaca atggggttga aatttgaacg tacagataat ttagataaac
     4021 ttttcgaatc atttgctgtc gatccggaaa aaacagttgc accaaaagaa gagatcgatc
     4081 gatttctaaa accacagcca caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta
     4141 agctcaaaaa aagacttacc agccaaactt ttatgaaaga gtttgactgg taagtctttt
     4201 ttattctttt tatgattcaa ttgcttgttc gattgctgcc cacgcttctt cttttccctg
     4261 ttttgtaacg gaagaaaaga gaataaacgt gtcattgcga tcaaaatcca acgctttttt
     4321 gatttcgctc tcatgcttgt tccatttacc acgagggatc ttatctgcct tagtggcaac
     4381 aacaattaca gggatatcat aatacttcaa aaattgatac atctggatat cttcttgaga
     4441 aggcttatga cgcaaatcta ctaaagaaac gaccgcacgt agctggtctc tagtcgttaa
     4501 atatgtctcg atcatctttc cccattttgc tcgctcagtt ttggatactt ttgcataccc
     4561 atatcccgga acatcgacaa aatgtaatgc gttctcgatc aaataaaaat tcaatgtctg
     4621 ggtctttcct ggcttaccgg aagtacgggc taaattcttt cgatcaatca atgtgttgat
     4681 aaaagaagat ttccctacat ttgatcgtcc tgccagagct atttctggca attgtgtatc
     4741 tggatattgt tttggtgcca ctgcacttat gataatttca gcttgatgga ctttcatggt
     4801 ctctctcctt aacaaggtga tgaataaagc aagcggctgt cgcaaaagcc aaaatgactt
     4861 ttacggcagc ctcttttcat caacccgctt ttttctctga atctttatag attactgttg
     4921 gctcaccaga caattcagct gcttcttttg tgataatcac tttttcgatg ttttcatttg
     4981 aagggatatc aaacatcaca tccatcatga tttcttcaat gatggaacga agtcctcgag
     5041 caccggtatt gcgttcgatt gcttttttcg caatggctct caacgcttca ggttcgaatt
     5101 caagttcggt attatctaga gaaagaagtt tttgatattg tttgactagc gcatttttcg
     5161 gctcagtcaa gatccgtacc agatcatctg ttgtcaattt ctctaaagct gccataacag
     5221 gtaatcgtcc gataaactca gggatcaatc cgaattttag taaatcttca ggaatgatat
     5281 gctgcatgac actttcgtct tcagaaagtt tttggttttg tgtaccaaat ccaatcgtct
     5341 tttcgcccat acgattcttc acaatcgttt cgatgccatc aaatgcacca cctacgatga
     5401 acaatatgtt cgtcgtatca atctgaataa attcttgatg tggatgttta cgtccgccct
     5461 gaggtggcac acttgcaaca gttccttcta gaattttcag caaggcttgt tggacacctt
     5521 cgccggaaac gtcacgagta atagatacat tttcgctttt acgcgcaatt ttatcgatct
     5581 catcaatata gatgatccct ttttctgcaa gttccacatt gtaatcagca gattgaagca
     5641 atttaagcaa aatgttttct acatcttccc ccacataacc agcttctgtc aaacttgtcg
     5701 catctgcgat tgcaaaaggt acattcaatg tttttgctaa tgtttgtgct aaaaatgttt
     5761 taccagaccc tgtaggtccg atcaagcaga tattgctttt ttgcagttca acatcatctg
     5821 tgttttcttg tgcttgctga ttgacacgtt tatagtgatt gtatacggca acagctaacg
     5881 agcgttttgc gcgatcttgt ccaatcacgt agttattcaa tacttcaaga atttctagtg
     5941 gtttagggac ttcagtgaat tcgcgtacag cttcttcata aaattcttca tcaatgattt
     6001 ctttacatag gtcgatacat tcattacaga tgtagacccc tggaccagct acgatctttt
     6061 tcacttcttc ttgggttttc ccgcaaaaag aacagcgtac ggtctcattg ctacttggat
     6121 tgtcatacat ggtcttcacc ccttaacttg caaaaagtat gcaagaaact atcaaagact
     6181 ttacagtctt ttactatcat aacatagttt agtaaaaaga aaaagtgttt aacaaaggat
     6241 caactgacct atacctttgc attttttact cttaacaaaa aataaggaac tgtgacaaaa
     6301 tcgtgtcaca gtcccttatt tcgaataaac aattttctag aagtagcttc ttcagaaatg
     6361 atttattact tgatttctat tctttcgctt ctgtcgctgt acctgtgatc aattcgattg
     6421 cttttttcat tgtcacatca tgttccagca tatcttctgt caacacgcgt ttgatttgtt
     6481 cgattggcat gttgtatgtt tctgataatt cttggatttc tgcattgatt tcttcttctg
     6541 ttgcttgaag attttcagca gctgcaattg cttcgatcac aagatttgtt cttgtgcgca
     6601 tttctgcttc accttcgaat tgtttatgca agtcttcttc tgtagaacca gttaattgat
     6661 agtacatttc aggagagata ccttgacgtt gcatgttgtt caagaattcg tccattgaac
     6721 gatgtacttc atcatgaacc atcacgtgtg gcaattcaac gatttctgcg ttttcaacag
     6781 ccatacggat cgctgcttca tcttttgcat cgtccgctgc tttttctttt gtttctacta
     6841 attctttgcg atatttttct ttcaattcgt ctaatgttga tacttcgtca tcaacatctt
     6901 ttgcgaattc gtcatctaat tcaggcactt cttttgcttt tacttcatgg attgtcactt
     6961 tgaagactgc ttcttttcca gcaagatctt ctgcttgata atcttctggg aaagttacat
     7021 ttacatctag actttcacct gatttatgac caactaattg atcttcaaaa cctggaatga
     7081 atgagcctga acctaattca agtgagtaat tttcgccttt tccgccatca aacgcttcgc
     7141 catctacaaa gccttcgaag tcgattacta ctgtatcgcc attttcagct gcagcatctt
     7201 ctttgataac taattcagct tgtgcttctt gttcacgttt gatacgtgca tccacgtcag
     7261 catctgttac ttcacggtct tgtttttcaa caactagatc tttatattgt cctaatttta
     7321 cttctggttt cactgttact tcagcagtga ttacccagtc ttgacctttt tccatgcttt
     7381 caacatcgat ttttggttga gaaacaggat cgatgccagc ttctttgaca gcagcttcat
     7441 aagcttctgg taatacagca ttcaatgcat cttcatataa agcttcttca ccgtacatac
     7501 ggttgaatac ttgacgagaa actttccctt tacggaatcc aggtacgttc aagctttttt
     7561 tcactttatt aaacgcagtt gttaatcctt tttggatttc gtcttgcgca atcgtaaatt
     7621 ttaaaacacc atcattcgtg cctttttttt caaatgttgc agtcatttaa ttccctccga
     7681 gtttagaaat tcattttata catcaaaaac gtttcatgca tacctttaaa tagtacacta
     7741 accctttcaa attgtaaaca tattgaatgg atagaatcct tgtttttcat aacattttta
     7801 tctttttcac tgataaaatc taatggaaga agatcatttg ctgcaaaatt ttttctcttt
     7861 tttttaaaaa atcttgtttt tcatcttctg taccttcata catcgttttt cctgtacacg
     7921 cctctacagt cagttgaaac cagcgcttcg ggtcttcgat gtatgtatca tagacaggat
     7981 aaacaattgc agattgcaac gtgaattctt cttttaacac ggaactaagc agcgcatctt
     8041 cattcccgta acgatctgaa aatacacata attgctggta acttgcctgc tgctgcggct
     8101 ttggcagatc ttttggtatg actttgacta acttttcttc aatcgtcaaa tagaaaacag
     8161 gatggcattc gttcaattgc ctaagtgtct ccagtaattt tgcccttgct aaaatatgga
     8221 cctctggcga tacaataagt ttactcgctg tttggataaa ctcatgataa ggaagctgtt
     8281 cgatctcatc aatagacgca agctgacgtg caggttccaa cgaaggaagt tcctccgatt
     8341 tattgtggag tgccttgatc ttctgcctct ccatttgtcc ggataattct tctgcaaatt
     8401 ggattttttc aatgacccgc tgctcgaaag cttcataaaa atctgtactt gctatcaatt
     8461 ttctagcata taaaaaatct tgtgtactga ttaacaagtc aaaataaaat tcagcaaact
     8521 cctcattttg aagatactgt ttttcatgca agagtgcttg cttcaatgct tcttttttct
     8581 cgtccaactc gtaaagacaa aaaacaagca ggctattagc ctcaaagctt tctttcaact
     8641 cgtaagctcg ttgaaataat tcttttgcct gctgatattg ctggtttgct agtgcttgtt
     8701 gtgccactcg cattaaattc tcatattttt ctggaaaatc aatcggcttc acgcagtgtc
     8761 ctcctcttgc tcatattcag ctcatgttca atagtagcaa aacaaaaaaa gcatgacaat
     8821 taaaaataac tgaagcaccc tctaaaagct agattttaga tctgactttt agaaggtgct
     8881 ttcaactgtt actgcttttt attgctcatc agctgttgac ttcctggttg aacgctttga
     8941 tcagattgat cgtaacaaca cttgtattag tgaaaaaatc gcccagatgt tgctttttct
     9001 gattaaagac tgctggaaga taccctaaca tcagcaaagg aaattttaga atgaaccggc
     9061 aagcaccttc tctaactaaa acggttgacc agctaagttc ttcttcatca aagctgatga
     9121 ctcgaatacc aaagatcatt ttcccaatcg tttggccatg attgaatttc gtcaataaaa
     9181 taaaatacgc tagataaata agtaaggctg tgaatccgta gagactcaga taagaatctg
     9241 aaaaattaag ccaacctaga tttttggcaa taccaagtgt taaacgcgta atagctccga
     9301 tacacaaaag atcgaccaag aaggcaaaca ttcggataaa aaaacctgca tagaaatact
     9361 taggaaaatc atttgctttt ggcggttcat ttcttgtgtc cgtatacttt ttccatttac
     9421 gttgtttctt tttgatttct tcgggcgtca aaggctctct ttcaaaagag ctcagtacct
     9481 tttgggaaaa gttggagtca ggtcctacag caggctgctg ttgcttatct ttttcttgtt
     9541 ccttattttc atttgaaagc tctgacatgc ttactcacct ccgtaataat acattgcttt
     9601 tggtgattcc gctgtcccta gattctcaag aacggataag atctgactag tctcagaagg
     9661 cttcaatccc tggaattctg ctagtttact tcctaaccat gtactagcaa acccagatgg
     9721 cgtggaagaa tattctacta gttccgcatc ttcaagctgt tgttcctttc gtatagcttt
     9781 taaagcatct tcaggaaatc ccaattcatc gactaaacct acttcttttg cttggacacc
     9841 atcataaatc cttccatcag caattttttt gacttcttct tcagatttat tccgtccctc
     9901 gctgacgatt gctacaaaac gactatatgc gctgtcgata tatgcttgaa gcaccgcttc
     9961 ttcttctttt gtctctggtc gcgttcctga acccatatct ttcaacgctc cgcttttaac
    10021 tgttgtatct tccacaccca gtttttctag aagtcctgaa taattcaatc ctgacatgat
    10081 cacaccaatt gagccggtca ctgtttcttc tgttgcaaag attttatctg cttgagcaga
    10141 aatatagtag ccaccgctag ctgccatgtt tttcatgctg acatacattg ggatatcatg
    10201 ttctttacga atcgtatcca atttcttagc gatttccgca ctttcgtaga caccgccacc
    10261 aggagaattt acttctaaaa agataccttt gactgttgga tcttcttcaa tcgccttcag
    10321 ttgttccata aaatttttat gattgtaacc ttcagaagca aataaacctg aagaccctcc
    10381 cgcagcaatc gttccatcta ctgataattt tacgattttt tgcgtactgt ctccctcttc
    10441 caacacattt ggagctagtt cttcatcccc atataaccaa ctgttcaatc cctcaatttg
    10501 atcgttttcc tgaggcttag tcaaactagc tgaaactaac gaaaccacaa ataatcctgc
    10561 agcaatcaga actgctgccc aacgtctttt attcattctt tctcctttct aatacaagtt
    10621 ttttatgacg atcacagttt ctgactgctc agaagtgtcg aaccgctctg ctttttcttc
    10681 ccgcgcatac ccggaaaacg actcttttgt taattcatct tctaccgtaa actcacgagc
    10741 ttgagcaact tccaaggcat caggtgcgat ttcttggatg ctatcccagc cagtatctaa
    10801 aaatattttc gtatcatctg atacaccagc tgatgcttcg aatttggaca attgtttttt
    10861 taacattccc agtgtcatga tctcaatttt tgaatccatt tttactcctc ctctatccga
    10921 ttactttcta ttgtaccgaa ctcctgaaaa agatgataga taaaaaccca ttattttttg
    10981 acataaaaag gacatttgac aaagaatttg atatatacca atttattttt tattcttttt
    11041 ttgttgacaa gaacattcgc aaaaggttat gattttaaat gtttccagat aaaaaacaaa
    11101 aaggaggata gttatacctt ttaggatagc gccaggtctg tgtaaacaga tgacgaggag
    11161 agggtttatc gaaagattcg gcggatggcc ctagggactg cactctacag aaaatccaca
    11221 aaaaatgtct gcaaagataa aacaaaagga ttttcctgca gctggaagca ttcatacgaa
    11281 ttcattatag aaagtaggat gttttttatg tgcggaattg ttggaatgat tggattaaaa
    11341 aatgtaacac caggattgat tgacggtcta gaaaaattag aataccgtgg ttatgattca
    11401 gctggtatct ttgtctcaga tggcacaact gattatctgg tcaaagcaca aggcagaatt
    11461 caaaatttga aaaacaaaat aaatattgat actacaggaa atataggtat tggtcataca
    11521 cgatgggcaa ctcacgggca accttctgaa gaaaacgccc atccacatac ttcccaaagt
    11581 ggacgcttcg tgctcgttca caacggcgta atcgaaaact ttgaagaact taaaattgct
    11641 tatttagcaa atgatcactt tattggtgag accgatactg agattatcgc tcatctaatt
    11701 gaaactttcg cgaaggatac tacaacgcaa gaggcgtttt taaaagcttt gcgggtcatc
    11761 aaaggttctt acgctttcgc tttgattgat cggacagcac ctgatgttat ttacgtcgct
    11821 aaaaataaaa gtccactatt aatcggactc ggcgatggat tcaatgtgat tgccagcgat
    11881 gcaatggcta tgcttgctca tacgaaggaa ttcgttgaga ttgaagatga agagatggtt
    11941 accgtcacat cagaaaaagt tattattcaa aattttgcag gtgacatcgt agaacgttct
    12001 tcattcgaag cacaagtaga tgcttcagat atcgagaaag gtacctatcc tttttatatg
    12061 ctgaaagaaa tcgacgaaca gcctattatc atgcgtcgta tcgctcaaaa atacgtaaca
    12121 gaagacaatc gtgtctctct tgatcaaaag ctggtcgata cattgtctga tagcgatcga
    12181 atctatatcg ttgcttgcgg aactagttac catgcggggt tagcaggtaa acagactttg
    12241 gaaaaattga cacagattcc tgttgaggtt catttagcca gtgaatttgg ctataacaca
    12301 cctcttcttt caaaaaaacc attctttatt ttcttgagtc aaagtggcga aacagcagac
    12361 agccgccaag tgctggtaaa aatcaaccgt ctcggctacc cttcacttac gattacaaat
    12421 gttgctggtt ctactctttc gcgggaagca tcctttacac tgctattgca tgctggaccc
    12481 gaaatcgcag tagcatcaac gaaagcttat actgcacaaa tagctgtact aactttatta
    12541 gccaaagcta tcggtgataa aaaagaattt gaaacttctc ttgtattcga tatcactcat
    12601 gaattgagtt tagttgctaa tgcgatggaa tcggtcatcg cccaaaaaga ttatttagaa
    12661 gagttggcag aagtatatct cagcgataca agaaatgcct tttacattgg acgtggtgct
    12721 gactacaacg tttctcttga agctgctttg aaattgaaag aaatcagtta tatccaggca
    12781 gaaggttttg ccgctggcga attgaaacat ggaacgattg ctttgatcga agaaggaaca
    12841 ccagtgatcg gaatcatcag tgaagaagtg actggtgcgc atactcgagg aaacctgaaa
    12901 gaagtcgaaa gccgtggcgc taaaacacta gttattgtct cagagggatt agaaaaggaa
    12961 agtgaccagc tggtgctacc aactgtccac ccttatctaa ctactctaac cacagtcgta
    13021 ccgactcaat tacttgcata ttacgcaacg ctattacggg gattcgacgt tgacaaacca
    13081 cgtaatttag cgaaatcagt gacagtagaa taataaaaga acaaggacca gtgtcagaca
    13141 tctgacactg gtccttgttc ttttattttt tatgaaaata tctcttaatc cagtccgatt
    13201 tcttctttga ctactgccgc gattttatcg acatagtaat taactttctc atcagtaggc
    13261 gcttctgcca tcactcgaag taatggttct gtaccggaag gacgaactag gattcttcct
    13321 tctgatccca tttcagcttc tgcctcttca atcacttttt tgattgccgg aacatccatc
    13381 gcaccgtttt tatcacttac gcggatattc actaattttt gcggatagat tgttacctct
    13441 gctgcaagct ctgataattt cttacctgtc tgtttcatga tattcagtaa ctgaattcct
    13501 gaaagcatcc catctccagt tgtgttaaaa tctaagaaaa tcatgtgccc agactgctcg
    13561 ccgccaaagt tataatcgtt tttgcgcatt tcttctacaa cgtaacgatc accaacttgt
    13621 gtaacgacat ctttcaaacc gattccttcc accgctttgt ggaatcctaa gttactcatg
    13681 acagtcgtaa cgatcgtatc ttttttcaat cgattttttt ctgccaagta tttagcacag
    13741 ataaacatga ttttatcacc gtcgacaatg tttccaagtt catccaccgc aataatacga
    13801 tcgccatcgc catcgaaagc tagtccagca tctgctcctt tttcaacgac catctcagct
    13861 aaacgttctg gatgggtaga gcctacgccg tcattgatat ttaacccgtc tggatttgtg
    13921 cccattgtat aaaaatctgt ttcaagatcg gcaaacaaac gattaacact tgttgaagtt
    13981 gctccgtttg ctgcatcgac acaaatggtc aaaccggaaa ggtcaccgtt gatcgtttgt
    14041 acaaggaatt gagaatattt caataagcct tctggaaatt cttctacagt tcccaatcct
    14101 tcagctgatg gacgaggcaa ttcatccact tcagcatcta ataatgcttc aatttctagc
    14161 tcttggtcat ctactagttt aaaaccatct gcaccgaaaa atttgattcc attatcttgt
    14221 gctggattat gagaagcaga aatcatgacc cctgcgcttg ctttttgcaa acgtgtcaga
    14281 taagcaacac ctggtgtaga gatcacacct agctggaaca cttcgatacc caccgataaa
    14341 agtcctgaaa tcagcgcttc ttctaacatt tgacctgaga ttcttgtatc ccgccctact
    14401 aacacgcgtg ggcgttttcc ttcttcttca tgctggctta ggacataacc tccgcaccgt
    14461 cccagcttaa atgcaagttc tggcgtaagt tccttgttgg cttcaccgcg tacgccgtct
    14521 gttccaaaat atttacccat agttgtaaaa ttactccttt aacttaattt tctgaggaat
    14581 ttgttctgct catttgtgtt gattcgcttg tttctgatgt ttcttctgta cttgtggtcg
    14641 agctgcttgt cccagattcc gaactggaag aaatggttgt tgtcgatggt tccgttcctg
    14701 aatcagcaga tgaaatactt ctactcgtac ttgtattttc ttgtgtccga ctgctcgtcg
    14761 aagcagagct actgcttgac gtttgcaaca cgggtgtaat ctccacacta actgattcgg
    14821 gcttgatact taacccctct tctactggta tttcaatttt acgagttgtt cgatgattga
    14881 tacctgagac attgatcggt acagcaatac tgtcaccgat cgatgcaagc aaatcgctag
    14941 agccgcttac ttctgcttcc agttttgaaa aactgaaatc atagctttct accccggcag
    15001 ctggtgttcc ttgctgtatc ccataaagtc gtaccgactt gcttggcttg atcgaatcag
    15061 ctctcactgt tacttgcact ggatcagaga cgatacttaa tacttcccct gctgcattca
    15121 gcgcctgtat tgggacctca ctattaatcc catcatctgt gaccatcgaa gcgtccacat
    15181 cagctactac tcgtttgatt tctgccaatg ttttgtcacc tgtagtgata gagacttttt
    15241 taggactaac ggaagtatca tcaagttcat atccagatgg tgtgaacgtt gaaggaatca
    15301 acgtttccac atcgaaatct tttattacct ttttctcaac cgttactgta atagtcgatg
    15361 gttcgatctt tgctgtgaca gcactgctca aattttgaat ttttagcgga acttcatgtg
    15421 ttccttcgcc cagcttagtg atatctgctg tgacgcggaa catccgtgta tcttcgtttg
    15481 cctctgcatt caactgaact cggtttgcac tggaaagttt gacagtcact gcattttcat
    15541 atccatggat atagtagcgc tttgaatcat aagaaatctt gatcggcacc cccgtaactg
    15601 tttgattata cgcttcattt ccagaaagcg tatttggaac ggactggcca tttgcattga
    15661 agtacagcgc aatactgaac agcaatgcaa taaaactgta aagaatgctt gtccgtttct
    15721 cttttgagat catttgcgat cccctccctt agtcataccg tctaggaaag attgaatcac
    15781 gtttttcttg ttttttgttt tctcttcagc aattagttct ctacgcaaga ttgccaaata
    15841 ttcttgctgg gataagccag ccatcagttc attatctaac gtgatgctga cgtccccggt
    15901 ttcttcagag acaacgatgg taatcgcgtc actgacttcg cttataccga cagctgcccg
    15961 gtgtcgagtc ccgaattctt tagggatcaa catactttct gatagaggaa gataggcgct
    16021 tgctactgcg atctttcctt cttttacaat aactgcaccg tcatgcagcg gtgtattagg
    16081 aataaagata ttgatcagca gctccccagt gatatctgca tctaaagcaa tccctgtctc
    16141 aatatactca tctaatccag tatgacgttc gatagtgatc aacgcaccga tttttcgctt
    16201 ggacatatat tggatggctt tgtcaaaaga caagatcatc ttctcatctt cctgctgttc
    16261 gctttttgat tgtttgaaaa gcgaactgcg tccaagatgt tccagtcctc tccggatttc
    16321 cggctgaaaa atcacgatag cagctatcac cccgtacgta atgacttggt tcatcagcca
    16381 agacaaggtg tgcagaccaa tgatttccgc tacgattcgg ataacaataa aaatagctac
    16441 acctttcaga agctgaatcg cttttgtgcc tcgcagcagt tggatcagtt tgtatacaag
    16501 ataccagaca accagaatat caagtatgtt caccaaaaaa ttcagagaga acaagtcacc
    16561 ggaaaacatt tgctgccaat agtccaaatt aaaaagttcc ccgatttgaa atgacataaa
    16621 aagcctctct tctcttttga gatttcctct ctagtatatc atatgtttta attttagagg
    16681 ctgatatgct cgtatttttc ttgagaaaaa cgcataattt tcaagaaaat tgcaagtatg
    16741 cgttttattt ttctctgcaa taaatatttt taaacttcag agaattattc attatatttt
    16801 ttctgttatt tttccttttt ttcttttgta gcttgttcca tttttttacg tcgttctgct
    16861 ctggcagctc ttctagcagc tcttctttct tctgcttcaa ggtccgtcgt tttttctttt
    16921 ctagtcttaa cgggtacatc gatagattcc tctggttcaa gtcgtttctt gattttttct
    16981 aattctcccg tcagtgcagc atagttgtaa aaacgtgttt tagcgtcgtg gacagtcttt
    17041 tcaaataaag cttttgcaag atttggattt gcgttatcta gcgaggaaaa gcgaacttgt
    17101 ttcctcatga aatcaggcat agcagagaaa tcaggttttt tgtagtccaa agtcattggg
    17161 ttcttgtttt tttcgaccat tgtagggtta tatcgatata atgaccaata accagaagaa
    17221 acagcttctt ttgcttcttt aagcgattct ttcattccgc caagcaagcc atgtgtgata
    17281 caaggcgtat aggcaataat cagagaagga ccaggatact tttctgcttc ttcaaatgcc
    17341 ttgatcgttt gcatttgatt ggcacctgaa gcaatttgcg ccacataaac atttccatat
    17401 gtcatcgcca tcattcctag atctttcttt gaagcatact ttccactagc cgaaaattta
    17461 gcgattgccg aagctggtgt tgcttttgag gtttgaccgc cagtattgga atagacttcg
    17521 ttatccaaaa caaggatatt cacgtctgct ccgctggcaa gtacatgatc gatcccgcca
    17581 aaaccaatgt cgtaagccca tccgtctcca ccaatcatcc attggctgtt ttttacgaat
    17641 agatcctttc tcttgtatat atcctccagt tcagggaaaa ttgatcgttc gctttcgatc
    17701 gcagcttgca gttttgctgc tctttgctgc gttccttcac tctcaaacat gtgtactaac
    17761 cagtcttcca tcaattctcg tagttcttct gatacttttg gcaatacttc tttgattgaa
    17821 cttgccaaag cctcgcgacg agtctgtgcc gctagaagca taccataacc aaattcagca
    17881 ttatcttcca ataaagaatt tgaccatgca ggcccttgac cacattcatt ggtcgtataa
    17941 ggagaagcag gagaagcacc gccccaaata gaagaacagc ctgtcgcatt cgcgatcatc
    18001 attcgatcac cgaacatttg tgtcaataat tttacataag gtgtttcgcc acagcctgag
    18061 caagcgcccg agaattcaag aagcggttga ttgaattgtg tagacaagac agtattcttt
    18121 cctttgactg gatttgcttt ttgtcttaat gtcattgcaa aagcccaatt cacagcttgt
    18181 tctttctgtt cttcataagg cttcatcact aaagcttttt cttttgcggg acaagcctct
    18241 acacacagac cacaacctgt acagtcttcc actgaaactt ggatacggta ttttacgccg
    18301 tctgcgcctc gcatttctct cacgataaat ccttctggcg cttcttccat ttcctcttca
    18361 tctgctaaaa atggacggat ggcagcatga ggacaaacaa aggcacattc attacacatc
    18421 gtacagcgat cactaatcca ctcaggtact tctaaagcga cgccccgctt ttccatagaa
    18481 gccgttccta aaggcatttc gccagttgtc attccatttg ctacaagtgt accaacagag
    18541 agttgatttc cttcctgtgc attcactggt tcaagtattt cttgtatata accagtgttc
    18601 cttttattga ttttttcagg catctccaac gttctccaat ggtctgggat cgcgacttga
    18661 tgaagcagtt cggtcgtccg atcgattgct tgtatgtttt tttctacgat agccattgat
    18721 ttatgtccat agcttttcag ggcttcttct tttagcagcg gaagcacctc atcaaaagga
    18781 atgatgtcag ctaatttaaa aaaagccgtt tccattgccg tattgatccg gcgtcccaaa
    18841 ccgacttctt gggccaatct catggcattg atcgtataga atctaatgtt attttcagcc
    18901 aagtaacgtt ttaattttaa gggcagatgc gtttctaatt gttcgtcgct ccaaagagta
    18961 ttcaataaaa aaatgccacc tggcttcaat cctttgacca agtcatagct atgcaggtaa
    19021 gctggtgtgt gacaggcaac aaggtccgca tgctcgacaa gatacgctga acgtatcggc
    19081 gtatctccaa aacgaagatg agagacagtc agaccgccag attttttcga atcataataa
    19141 aaatagcctt gcgcatattt atcggtgtga tctccaataa tcttgatagc tgatttattt
    19201 gctccaactg ttccgtcaga tccaaatccc caaaacttgg cctggaatgt ttgcggttct
    19261 gtcaaattca gactttcctt tggttccaaa gattgataag tcacatcatc cacgatccct
    19321 atggtaaacc gttttttgcg tatgcttggg tcttttttca attcatcaaa aactgctgag
    19381 atttggtcgg gagtcacatc tttagacccc aatccgtagc gaccaccaat aacagccggg
    19441 cgaagttcag aatcatataa cgcactttga acgtctaata agagaggttc tccacctgcc
    19501 cctggttctt tacttcgatc aagaacagca attgttttta ctgtatgagg caacttttgc
    19561 acgaatgttt ccactggaaa aggacgataa agatggatat tcaaaaaacc tgttttccgt
    19621 ccgtttgcat tcaagtaatc aatcgtttgt tcaatcgtct gtgcaactga tcccatagaa
    19681 acaatgactt cttccgcatc gtctgctccg taataagtca ccaagtcata agaagttcct
    19741 cgtaatcggt tgatctcttt catatatttt tgaacgattt caggtaattt atcataatag
    19801 gggttgatcg tttcccgttg ttgaaaatgg atgtctggat tctggttcgt accactaaca
    19861 gtcggatgat tgggattcat ccctcgttta cgaaatgaag ctaatttctc ctgattgatc
    19921 agaggagcta attcttcata ttccaaaact tcgatttttt gaatctcgtg actcgtacgg
    19981 aatccatcaa agaaattcaa aaaagggact tctccttcaa tagaagcaag atgggcaaca
    20041 ggtgctaaat ccatcacttc ttgtacactg ctttcagcta acatagcaaa tcctgtttgt
    20101 cttgcagcca tcacgtctcc gtgatctccg aaaatattca acgcatttgt cgtcactgcc
    20161 cgactagcaa cgtggaaaac acttggcagt agttccccag caatcttgta catattcggt
    20221 atcatcaaaa gcagcccttg tgaagcggta tatgtagttg tcagtgcacc agttttcaat
    20281 gatccatgga ccacaccagc tgctcctgct tcggattgca tttctactac ttttactggt
    20341 tgaccgaata cgttttcttt tcctgctgct gaccattgat ctactaattc agccatggtc
    20401 gaactgggtg ttattggata tattgcagca atctcggtaa atgcataaga aatatatgcg
    20461 gctgccgtat tgccatccat tgttttcatt tttcgcatga ttcatcatcc ttgttcatat
    20521 tctctcttat tatctgttcc attataacgt aaaacatgcc atttttttct tttatttata
    20581 aaaatatttt acatgtatct atttgaattg gtcatctaat ttttagacaa acagtctaaa
    20641 tcgttgcctt tttttgtcag acatgtataa actataagcg aaagaggtgt tttaacatgg
    20701 atatatcagt gattgatgca acgaaaacaa atacacaaaa aggaatcctt tatggttcat
    20761 cgaatgctcc taaaaaaatg gtcgaattca tcaacttagc ttgtccgtat tgccgacaat
    20821 ggtttgaaga atcttatgag ctattagaag aagcagtcca aagcgggcaa ttgcagcgag
    20881 tgatcaaatt gttcgataaa gaaaaagaaa gcttacagcg agggaacgtg atgcaccggt
    20941 atctcacaat cagcgatggc caaaaagcga tcaaagagat caaacaaata ttcgatactc
    21001 aagacgagtg gaaacatctt tctctccaag aagtagcgga ttttgcagta gataaactaa
    21061 agctgacaga acaaaaagac gaacagctga gccaagctgt catcaacgaa gctgagcaag
    21121 cacatattcg atttgttcca actgtcatct tgggtaaaga aatctttgat gaatctattt
    21181 caatagagga attaaaagaa ttgatccaaa caaaataaaa aaataa
//
LOCUS       DACCSK010000046        21066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCSK010000046 DACCSK010000000
VERSION     DACCSK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.046"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(23..604)
                     /locus_tag="IVP39_001452"
     CDS             complement(23..604)
                     /locus_tag="IVP39_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7586531.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(610..1869)
                     /locus_tag="IVP39_001453"
     CDS             complement(610..1869)
                     /locus_tag="IVP39_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7586532.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1871..2917)
                     /locus_tag="IVP39_001454"
     CDS             complement(1871..2917)
                     /locus_tag="IVP39_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586533.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3152..3427)
                     /locus_tag="IVP39_001455"
     CDS             complement(3152..3427)
                     /locus_tag="IVP39_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7586534.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3670..4980)
                     /locus_tag="IVP39_001456"
     CDS             complement(3670..4980)
                     /locus_tag="IVP39_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7586535.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5162..6391)
                     /gene="rpsA"
                     /locus_tag="IVP39_001457"
     CDS             complement(5162..6391)
                     /gene="rpsA"
                     /locus_tag="IVP39_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7586536.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6526..7206)
                     /locus_tag="IVP39_001458"
     CDS             complement(6526..7206)
                     /locus_tag="IVP39_001458"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7586537.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7255..7890)
                     /locus_tag="IVP39_001459"
     CDS             complement(7255..7890)
                     /locus_tag="IVP39_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7586538.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7956..9359)
                     /locus_tag="IVP39_001460"
     CDS             complement(7956..9359)
                     /locus_tag="IVP39_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7586539.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(9346..10377)
                     /locus_tag="IVP39_001461"
     CDS             complement(9346..10377)
                     /locus_tag="IVP39_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586540.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10408..10629
                     /locus_tag="IVP39_001462"
     CDS             10408..10629
                     /locus_tag="IVP39_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7586541.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10794..11531)
                     /locus_tag="IVP39_001463"
     CDS             complement(10794..11531)
                     /locus_tag="IVP39_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7586542.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11684..13024)
                     /locus_tag="IVP39_001464"
     CDS             complement(11684..13024)
                     /locus_tag="IVP39_001464"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7586543.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13261..14784)
                     /locus_tag="IVP39_001465"
     CDS             complement(13261..14784)
                     /locus_tag="IVP39_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7586544.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(15162..15773)
                     /locus_tag="IVP39_001466"
     CDS             complement(15162..15773)
                     /locus_tag="IVP39_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7586545.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15896..16021)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16103..16819)
                     /locus_tag="IVP39_001467"
     CDS             complement(16103..16819)
                     /locus_tag="IVP39_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7586546.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16825..17394)
                     /gene="scpB"
                     /locus_tag="IVP39_001468"
     CDS             complement(16825..17394)
                     /gene="scpB"
                     /locus_tag="IVP39_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7586547.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(17378..18175)
                     /locus_tag="IVP39_001469"
     CDS             complement(17378..18175)
                     /locus_tag="IVP39_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7586548.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(18145..18516)
                     /locus_tag="IVP39_001470"
     CDS             complement(18145..18516)
                     /locus_tag="IVP39_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7586549.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18535..19422)
                     /gene="xerD"
                     /locus_tag="IVP39_001471"
     CDS             complement(18535..19422)
                     /gene="xerD"
                     /locus_tag="IVP39_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7586550.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19518..19991)
                     /locus_tag="IVP39_001472"
     CDS             complement(19518..19991)
                     /locus_tag="IVP39_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7586551.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(20096..20959)
                     /locus_tag="IVP39_001473"
     CDS             complement(20096..20959)
                     /locus_tag="IVP39_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7586552.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
BASE COUNT         5936 a         4505 c         3521 g         7104 t
ORIGIN      
        1 tttagtacta tactattttt aactaattgt tatgttcact tacccggtta cttttctttt
       61 gtgtctgcaa aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat
      121 ctccatcttc tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt
      181 ctttttcttg aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc
      241 aagaagaaaa tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt
      301 cgaatataca ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct
      361 ggtctgtgtc cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag
      421 atagatcact gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat
      481 tgcttaaaat cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc
      541 taaaagatac atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta
      601 gcatttttct cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg
      661 ttcaagtgtt aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct
      721 taagaaaaag gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg
      781 ttcatagtgt acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt
      841 ccattctgca taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc
      901 atatctttca tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc
      961 tcctacttct aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa
     1021 agcattttcc gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc
     1081 ttctaacact gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata
     1141 tagatataac gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc
     1201 ctgctgattt tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt
     1261 cctttcttct ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat
     1321 acttaaggat tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct
     1381 tgccaacaat gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa
     1441 ttcagctagc gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa
     1501 ttttgcttca ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa
     1561 gctttgcgga tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc
     1621 gatctcatta tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg
     1681 gtattggttg atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag
     1741 caattcttcg cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa
     1801 aaagatttgt gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt
     1861 tgtattcatt tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc
     1921 tcaataattc tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa
     1981 caagtatttt tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg
     2041 aaatctgttc cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt
     2101 ttagttggaa atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta
     2161 aaaatttttt catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt
     2221 caaacagttt accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt
     2281 cttcgatcat cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat
     2341 acagattttg caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt
     2401 caatctgctt tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg
     2461 gcagattgat ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg
     2521 ttaatgctcg gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg
     2581 ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga
     2641 taaacatgga ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg
     2701 ttaaatgatt gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa
     2761 ataacgggtt gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga
     2821 ttggctgatc aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt
     2881 tcgtcaacgc atcttctact tgataatcaa gttccatacg catccctcct cttcttattt
     2941 ttattttacc attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact
     3001 tgcaaataaa taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg
     3061 gaatctctgt ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag
     3121 ccctagagcc acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac
     3181 ctggtttgaa tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt
     3241 tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa
     3301 ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag
     3361 ttgcatcttt tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt
     3421 ttgccatgga tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa
     3481 actttctgag tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat
     3541 cacgatatca tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt
     3601 agtcatagaa acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg
     3661 gtaaaatgcc tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa
     3721 tgcttttctg atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc
     3781 attgacaaaa acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt
     3841 caggcgtttt cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc
     3901 attcaacaca gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc
     3961 aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc
     4021 aagataccgg aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt
     4081 ctttacaaga tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta
     4141 acccgctact cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac
     4201 gatatcagaa cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc
     4261 gtatactttt ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact
     4321 ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat
     4381 tacacgttct tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc
     4441 aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt
     4501 cactgcttca tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg
     4561 atcgcctagc cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac
     4621 tttattgact gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc
     4681 atctgcgtct gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc
     4741 aatcgcgatt tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat
     4801 tccgcctgtg tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg
     4861 atcacgagtt actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt
     4921 aaaaatcgta gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat
     4981 attatttcct cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt
     5041 caagcataga ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt
     5101 ttcaaaagaa aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca
     5161 attatttttc agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta
     5221 aaatgtctcc catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt
     5281 ttggttcttc ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat
     5341 gttcttctgg atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt
     5401 catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc
     5461 cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata
     5521 ctgtgccaac tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga
     5581 ttgacaagga aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat
     5641 cattgacagc caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat
     5701 ggactagacc gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa
     5761 cacgtccact gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt
     5821 ctctttcgtt ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga
     5881 tttcgatgat tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat
     5941 gatcttccac cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc
     6001 ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga
     6061 tatcttccca aactttttta gcatctaaac ggcgttttga aagtagatag ctgccgtttt
     6121 ctttatcttt gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta
     6181 cttcgctgat atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa
     6241 ctccagcccc ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct
     6301 taacaacatc gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt
     6361 tgttctcttg attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc
     6421 cgtaagtacg gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt
     6481 tcgttttcct tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata
     6541 accttttttc aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt
     6601 cgtatcgata cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc
     6661 ataatggtct ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt
     6721 ttcttggttc tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat
     6781 tttcacttct gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc
     6841 accgcgttcc cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc
     6901 agagacttca gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt
     6961 tgatgctatg aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt
     7021 gatcagcgaa actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata
     7081 agttactgcg cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa
     7141 tatcttggca acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg
     7201 tgtcatgata taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt
     7261 aattcttaat tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc
     7321 aaacaattga tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc
     7381 ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt
     7441 gctgcttgaa tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc
     7501 tgcaatactg ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat
     7561 tgtactagat tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact
     7621 ttctactact gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc
     7681 atttctgatc cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa
     7741 aaatagcgaa agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga
     7801 aacttcatca tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc
     7861 tgtagattga tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta
     7921 ttataccata ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat
     7981 aaatttagta aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt
     8041 ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt
     8101 ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga
     8161 agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa
     8221 ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag
     8281 ggagcttgct ctgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa
     8341 agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca
     8401 ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct
     8461 ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat accaaatgca
     8521 ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt
     8581 tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga
     8641 tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt
     8701 tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata
     8761 ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt
     8821 atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct
     8881 aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga
     8941 gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct
     9001 aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga
     9061 tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt
     9121 ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac
     9181 acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc
     9241 gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct
     9301 tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt
     9361 ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa
     9421 ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc
     9481 caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa
     9541 ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat
     9601 ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa
     9661 attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat
     9721 ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaaccgctg actcatcatt
     9781 gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata attggttagc
     9841 taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc
     9901 agtatcaatc aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa
     9961 agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga
    10021 aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg
    10081 gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg
    10141 cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa
    10201 aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc
    10261 gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag
    10321 agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt
    10381 attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc
    10441 tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa
    10501 ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa
    10561 gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat
    10621 aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata
    10681 agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc
    10741 tatctatagt taattgacta gttttttcac ggaccctata ggacccattt atattatttc
    10801 aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa
    10861 atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg
    10921 ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct
    10981 tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct
    11041 gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg
    11101 tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg
    11161 atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc
    11221 actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct
    11281 tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa aataagtaat
    11341 ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt
    11401 ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag
    11461 taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta
    11521 aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat
    11581 gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga
    11641 cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac
    11701 gatacacagc ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt
    11761 cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact
    11821 ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag
    11881 taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata
    11941 atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta
    12001 atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt
    12061 ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt
    12121 catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta
    12181 agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag
    12241 cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt
    12301 caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct
    12361 tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag
    12421 aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa
    12481 ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat
    12541 atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg
    12601 tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag
    12661 gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga
    12721 atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga
    12781 taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt
    12841 taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca
    12901 cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc
    12961 ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt
    13021 tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc
    13081 tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac
    13141 aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttatccac gtgcttatcg
    13201 atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt
    13261 ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa
    13321 tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat
    13381 tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt
    13441 gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt
    13501 tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa
    13561 tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa
    13621 gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac
    13681 tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga
    13741 ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata acatagtaat
    13801 ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac
    13861 cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt
    13921 tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac
    13981 atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt
    14041 catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga
    14101 tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg
    14161 aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa
    14221 aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc
    14281 tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat
    14341 cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg
    14401 aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag
    14461 tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat
    14521 tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt
    14581 gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga
    14641 tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt
    14701 aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa
    14761 ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat
    14821 tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt
    14881 tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt
    14941 taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc
    15001 ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca
    15061 aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt
    15121 ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga
    15181 cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact
    15241 aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt
    15301 acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag
    15361 taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt
    15421 cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa
    15481 taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa
    15541 ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca
    15601 actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg
    15661 atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg
    15721 atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat
    15781 cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact
    15841 ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta
    15901 tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg
    15961 caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa
    16021 ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct
    16081 aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt
    16141 tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt
    16201 cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat
    16261 tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata
    16321 aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag
    16381 gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt
    16441 catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc
    16501 ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa
    16561 gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt
    16621 tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag
    16681 tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac
    16741 gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta
    16801 ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa agaatagatc
    16861 tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa
    16921 tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc
    16981 tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc
    17041 tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata
    17101 agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc
    17161 atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt
    17221 ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac
    17281 taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat
    17341 cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg
    17401 tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga
    17461 tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag
    17521 aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt
    17581 cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt
    17641 tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt
    17701 tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt
    17761 cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt
    17821 tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata
    17881 attcttcatc gtctgtgacg aattcttgga caggaagcaa tgtcttgctt ttgattgcca
    17941 tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg
    18001 tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga
    18061 tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt
    18121 taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc
    18181 aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa
    18241 ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc
    18301 atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt
    18361 tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat
    18421 ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc
    18481 aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc
    18541 ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat
    18601 ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc
    18661 attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta
    18721 aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc
    18781 tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg
    18841 tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg
    18901 ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa
    18961 tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc
    19021 acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc
    19081 tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat
    19141 cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga
    19201 gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg
    19261 gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg
    19321 caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc
    19381 aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc
    19441 tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga
    19501 aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata gcattcctga
    19561 cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact
    19621 acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag
    19681 catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat
    19741 ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga
    19801 taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct
    19861 gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt
    19921 gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca
    19981 ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa aaagatataa
    20041 cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg gattctcaat
    20101 tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa
    20161 gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc atgatttctt
    20221 ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct aaaatggtta
    20281 aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat gaaagattca
    20341 gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct agacgaggtt
    20401 cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt aaaacgtacg
    20461 tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc atttcttctg
    20521 ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc caaatacggt
    20581 cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc catagctctg
    20641 tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga ccgatatcga
    20701 caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac
    20761 ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg taaccgaatc
    20821 cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca aaggtttgtc
    20881 cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct gtaaatatag
    20941 ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct taatacattt
    21001 ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt tggaaaggtg
    21061 aaaaaa
//
LOCUS       DACCSK010000047        19463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCSK010000047 DACCSK010000000
VERSION     DACCSK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.048"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(42..1243)
                     /locus_tag="IVP39_001474"
                     /pseudo
     CDS             complement(42..1243)
                     /locus_tag="IVP39_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1360..1875
                     /locus_tag="IVP39_001475"
     CDS             1360..1875
                     /locus_tag="IVP39_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7586553.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2074..2520)
                     /locus_tag="IVP39_001476"
     CDS             complement(2074..2520)
                     /locus_tag="IVP39_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7586554.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2567..4045)
                     /gene="msr"
                     /locus_tag="IVP39_001477"
     CDS             complement(2567..4045)
                     /gene="msr"
                     /locus_tag="IVP39_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7586555.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4399..5028)
                     /locus_tag="IVP39_001478"
     CDS             complement(4399..5028)
                     /locus_tag="IVP39_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7586556.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5012..5476)
                     /locus_tag="IVP39_001479"
     CDS             complement(5012..5476)
                     /locus_tag="IVP39_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7586557.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5963..7231)
                     /locus_tag="IVP39_001480"
     CDS             complement(5963..7231)
                     /locus_tag="IVP39_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7586558.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7462..8199)
                     /locus_tag="IVP39_001481"
     CDS             complement(7462..8199)
                     /locus_tag="IVP39_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7586559.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYSDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8266..8835)
                     /locus_tag="IVP39_001482"
     CDS             complement(8266..8835)
                     /locus_tag="IVP39_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7586560.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            9013..9834
                     /locus_tag="IVP39_001483"
     CDS             9013..9834
                     /locus_tag="IVP39_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7586561.1"
                     /translation="MSRAAKSLHITQPTLSKQLKDLEEELGVTLFTRGSREIQLTDSG
                     RYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAISFISDSLHSLIKAHPDI
                     QFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLPAQDRWGLLIHRNHPLAK
                     QTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVGTYNLLYNASLMVDNGTF
                     GALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSPAARKFLDVLTEKYS"
     gene            10176..11048
                     /locus_tag="IVP39_001484"
     CDS             10176..11048
                     /locus_tag="IVP39_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7586562.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHTETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11045..13003
                     /locus_tag="IVP39_001485"
     CDS             11045..13003
                     /locus_tag="IVP39_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7586563.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSDKA"
     gene            complement(13254..14105)
                     /locus_tag="IVP39_001486"
     CDS             complement(13254..14105)
                     /locus_tag="IVP39_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7586564.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14228..14695
                     /locus_tag="IVP39_001487"
     CDS             14228..14695
                     /locus_tag="IVP39_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7586565.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14958..15440)
                     /locus_tag="IVP39_001488"
     CDS             complement(14958..15440)
                     /locus_tag="IVP39_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7586566.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LSESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15540..16403)
                     /locus_tag="IVP39_001489"
     CDS             complement(15540..16403)
                     /locus_tag="IVP39_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7586567.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17001..17849
                     /locus_tag="IVP39_001490"
     CDS             17001..17849
                     /locus_tag="IVP39_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586568.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18020..18361)
                     /locus_tag="IVP39_001491"
     CDS             complement(18020..18361)
                     /locus_tag="IVP39_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP7586569.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18447..19154
                     /locus_tag="IVP39_001492"
     CDS             18447..19154
                     /locus_tag="IVP39_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7586570.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19141..>19463
                     /locus_tag="IVP39_001493"
     CDS             19141..>19463
                     /locus_tag="IVP39_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7586571.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
BASE COUNT         5830 a         4003 c         3468 g         6162 t
ORIGIN      
        1 atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc ttcatttgta
       61 gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat ggcacctgca
      121 aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat aaatggtgct
      181 aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt tcgttgcaga
      241 cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg gaaaaatcct
      301 gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc taataacagc
      361 aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt aaacttccgc
      421 tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca gccaaaaaat
      481 acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata cgcaagtaaa
      541 gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag tttatgattg
      601 cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc agtttcaggt
      661 aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga
      721 taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc cattccgcag
      781 ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa aatactgacg
      841 atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc ggcacgtaac
      901 ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac aacgatgata
      961 aacaatcctg tcatcaatgt tttttcaaac cgatccgact agataagaaa cctgtaaata
     1021 gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt tcagctggcg
     1081 tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc actcccataa
     1141 aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt gtttcttcat
     1201 ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc tctctattag
     1261 catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga aactttactg
     1321 tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt agcctatata
     1381 ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca gctgatcaca
     1441 attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt agaccagcag
     1501 ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta ccacttgact
     1561 gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc tcctacgcaa
     1621 aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac aacagctact
     1681 ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa tcaaaaaaaa
     1741 ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt gatccagcgc
     1801 agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga acaaatgcag
     1861 cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga agagcaaatt
     1921 atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg acaggccaaa
     1981 acctatcgtt cggatcacta tttttctaga actgcattca agagactatg acaaaaagca
     2041 tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa ttgcttttag
     2101 cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg caatcacatt
     2161 cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt gttccatgat
     2221 cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg aaatagttaa
     2281 gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt ttttcgccag
     2341 cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca ctagaccaat
     2401 caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc tcatccccaa
     2461 gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat ttgtaggcaa
     2521 tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa aaatttctcg
     2581 taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc gtacgctcga
     2641 caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc attagcttct
     2701 ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac agcaacacat
     2761 tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc ccacttaatg
     2821 tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc aaactacgga
     2881 caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat tcttcagatg
     2941 tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag agaaggattc
     3001 cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca ttttcaccga
     3061 caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt aatgttctcc
     3121 cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg agttcaagag
     3181 attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt tctcctattt
     3241 tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt acactgtctt
     3301 tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt ttactggaca
     3361 ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc aatcgttttt
     3421 tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat tccttttgag
     3481 tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt acttcccaga
     3541 ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc agcgtaccat
     3601 aataataacg aagctcttca atcagtcgat caatactttt tctatctaga tgggttgttg
     3661 gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac tcgatctttc
     3721 gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac tggaaatgac
     3781 tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga ggcaggtagt
     3841 tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg gctattagtt
     3901 tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga tcttgctgat
     3961 agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact tgtagatttg
     4021 ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta aaacaacaat
     4081 tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc acatcttttc
     4141 ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt tcacctattc
     4201 tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta tacatgtctt
     4261 ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat gcagtcattt
     4321 ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa tagctactga
     4381 atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta aacgaacaga
     4441 ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa cataacggtc
     4501 tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa ttggatttgt
     4561 ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc tagaatcgac
     4621 tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa tgatcgcagc
     4681 taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt ggttatagat
     4741 ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg cagaagaagt
     4801 atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg cttcctcgct
     4861 ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag tcgtcgcttc
     4921 tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat atacgcgaac
     4981 ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta ttattaacct
     5041 ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa agtgttagaa
     5101 attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta tctttatggt
     5161 ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca tacacttccc
     5221 ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat accttcagat
     5281 gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt tgtagcaatg
     5341 gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata ccgatatttt
     5401 cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa aggtgactaa
     5461 tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg attgacttgt
     5521 caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa aaaaaacgct
     5581 ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa aaatgataaa
     5641 gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa gatagctctt
     5701 ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc aagaatcccg
     5761 tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta tgacaagatg
     5821 ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg taataagttc
     5881 aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac ttgatgcagg
     5941 gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca tatctccagc
     6001 ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg gtaccagttt
     6061 tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa aaacttagac
     6121 cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc attgtgtaat
     6181 gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg tttggtgctt
     6241 gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt tctccggcat
     6301 tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc ggagaagttg
     6361 aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg ttcaatgtag
     6421 aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca tctttgtaag
     6481 agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga aaagaagtcg
     6541 tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat tttctaaaag
     6601 cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc gtaacagatt
     6661 ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa gccaaagtac
     6721 tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca tataagtcat
     6781 tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct tgttgagaat
     6841 tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca gacaaatctg
     6901 cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt tcttctgcat
     6961 tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt tgttctgttg
     7021 tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct gactgcaaag
     7081 tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga gttgcgacag
     7141 tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt cctaaaagaa
     7201 taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt atgaaacttt
     7261 tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat ttaaataaca
     7321 tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg aagaatattc
     7381 ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa gtaattctct
     7441 ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa tcatgttact
     7501 gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta tatcttcgga
     7561 ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt tgtcgctgat
     7621 ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt tgatgttatt
     7681 ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt ttgttccgca
     7741 ataaacggcg gaatcagaat aaacaacatg tcccgccaca gaatcagttg aaatgatatg
     7801 cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta acacacccat
     7861 gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta atggagcggt
     7921 tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt ccatcgctaa
     7981 atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa tatatagctc
     8041 agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg cagctataac
     8101 tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc cgcttgaagc
     8161 gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct cctgttttga
     8221 aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa taatccgttc
     8281 gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg ggacatcttt
     8341 cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc ctggcagaat
     8401 agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta ctgacaagcc
     8461 tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac gaggaccaat
     8521 caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt gacgattgat
     8581 aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag gcagcagaac
     8641 agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc ggatatctgc
     8701 tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag cttggatctg
     8761 atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga tttgtttttc
     8821 gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga cggcttatca
     8881 tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag tggtatgctt
     8941 ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt agcagtagct
     9001 agaaaaaaaa atatcagccg agcagcaaaa tctctgcata tcacgcaacc tactctctca
     9061 aaacaactga aagatttaga agaagaactt ggtgtgacac ttttcactcg gggctcccga
     9121 gagatacagt tgacggattc tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg
     9181 ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa tcatctctgg agaaatctac
     9241 attggcggag gcgaaactaa agcaatctct ttcatttctg atagccttca ttcgttgatc
     9301 aaggcgcatc ctgatatcca gtttcattta tatagcggga atgcagatga tatcatagac
     9361 aaactggata gcggattact tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa
     9421 taccagtcat ttcgactgcc agcccaagat cgttggggac ttttgataca tcgaaatcat
     9481 cctttagcaa agcaaaccgt catccatccg caggaactga aaactgttcc tttgatcatt
     9541 tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc taggtttcag catagatcag
     9601 ttatcgatcg tcggaacata taacttattg tacaatgctt ctttgatggt cgataacggg
     9661 acttttggtg cattagctct agatgggatc atcaatactt atgggacgga aaaaatattc
     9721 atcccactcg atccgccatt acaagcttca ctgaatctca tttggaaaaa agaccagcct
     9781 ctttctcctg ctgctcgtaa atttttagat gtcctaacgg agaaatacag ttaagtagag
     9841 tttttttcct tcgctttcag ctttttaaca acgaataaga taagcaaaag atccaacgat
     9901 taaataacat aagtttctta atttatttct tccttttatt tctgataatt ccagtaaatt
     9961 actataatta taaaaaaaga attgacaaaa cataactgtc tatataaaat tgattacgtt
    10021 aacaataatt cgtttcatgt gactagatca tactaggcat gaaaaatctc tgaggaacat
    10081 ggtttcccta ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac tggtctagca
    10141 aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc
    10201 aaaatgcagt attggtagat gacggtggac aggaaaaagt agctattgga aaagggattg
    10261 gatttgacag aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg atatttgtca
    10321 tggaaccaga aggtcaaatc aagttgcaaa atttacttaa tcagattgat gaacgctttt
    10381 tatttgcagc agaaacgatc attgatcata cggaaactgt tttgatggaa aagttgaatg
    10441 aacatgtatt gatcgctttg accgaccaca ttgctttttc tgcggagaac attaataacg
    10501 gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt gctttacagt gatgaattca
    10561 gtattgctca atgggcagta gagtatctga caaaagagct tgggattcca tatacttatg
    10621 acgaagcagg atatatcgca atccatattc acagcggacg aagcggtcgg aacaataacc
    10681 atcgcagtat ccgtgaagtg acgattgtat ctgacatcat ccatttagtg gaatcagaat
    10741 tggaaacaga tatccattca gaaccatttt cactgaatta ttcaagactg gtgaatcatt
    10801 tacgactgct tctacaacga acccatgcgc aacaatatgc tgttttagac acggaaatcg
    10861 tagaaatggt caaacgaaaa tacccagaaa gctataagat ttctaaaaaa atccgtgtgt
    10921 tgctgaccaa agaataccag ctcgcgatca cgaaagaaga actgggttac ttggccatcc
    10981 atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa
    11041 aaaattgaaa gcatatctac aacgaatggg acgttccctt caacttccag ttgcggtctt
    11101 acctgctgct gctttactag tagggatcgg aaactggtgg gcatcttata gtaacgacat
    11161 tgtcgctcat tttttacaag ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt
    11221 tgccgttggg ttagcactag ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg
    11281 tttggtcgca tttgaagttc cagtaaatgt tttaaaaact gattcagtag ctactttatt
    11341 gaatatcaaa ccagaatcag ttgatccatc ttttgctcag atcagtaacg tatttattgg
    11401 tattattgcc ggattgattg cggcagctct ttataatcgt ttccacgaag caaaattacc
    11461 gatggcttta tctttcttta gcggcaagcg tctagtgcca atcttggctg cctttgtcat
    11521 gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg ctttagtttc
    11581 ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg gcttcttcaa
    11641 ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct ggtttgacgt
    11701 agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg
    11761 aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag gagcatatgc
    11821 tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga tgctagcagc
    11881 agcttttgct tcattcttta ctggtgtgac agaaccactt gaattttcat ttatgttcgt
    11941 tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag cagtgagtgc
    12001 attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt acttcctatc
    12061 tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat tagtaacagc
    12121 agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga tgactcctgg
    12181 ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag acaataaata
    12241 tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg taacttcgat
    12301 cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa
    12361 taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa atgcgcaagt
    12421 catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac atggtggtgc
    12481 tgcagcacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg ccggtgaaac
    12541 gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca ctgaagtttc
    12601 agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac cagaaaatgg
    12661 agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga aacatgctgt
    12721 cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca acactgttga
    12781 cttaaaaggc gaaccattca ctttatatgt agaagaaggt caaaaagtcg caagaggtca
    12841 attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca cggatatggt
    12901 cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg cacgtgatgt
    12961 aaaagcaaat gataaaatcg gctctgtttc cgacaaagcc tgagccatcg tcttccccag
    13021 acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag ctcctttttc
    13081 cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcca aacgaaacat
    13141 atgaagagct attttttatt tgactcttct tattacttga tgcagaacag ctttttcaca
    13201 aaatttttct tcatactcaa aactaatcgg ctttttcaca acctctttat acattattcc
    13261 tgatccaata tttcttttaa tgctgcttca agagaagtta atggttttcc caatactttt
    13321 tctaaatcat cagaggaaat agctaactga tcctttttaa tatcatgttg gatcgctaaa
    13381 aacatgtcag ctgcttcttg agggaatccg gcttctttta gattttcgac aaacgctgca
    13441 tcacttgctt caataatttc taactcttta cctgtcgcac gctctaatgc cttggctaat
    13501 tcttcatagt cagttagatt gccggataat tctaagattt ctggagagtc actttctact
    13561 agtacttttg ctgcagcttc tgcatattca cgtttcaatg cccaacctgt ttttccattt
    13621 cctgcagcat aaacgaattg accagttttt aaagcctgcc caatgatagg taattcattc
    13681 tctacatacc aattattccg taaaaatgta tgggaaatac ctgaatcctt aatcattttt
    13741 tcagtgaatt ggtgatctgg cgctaataca ctgatcgagt gatcggcatc tgcaaaactt
    13801 gtataagcga tataagatac cccagctgct tgtgctgcct tcactacatt cttatgttcg
    13861 gcttgacgat ttcctggaac gccagagaca aaaagcaatc gatctattcc tttcaacgct
    13921 tctttcattg agtctaaatc atcataatca ccaatacgta tattaaatcc agcttctttc
    13981 agtgcttctc ctttttcttc actacgtact agtacataga tttcattttg tggaaccatc
    14041 ttttttaaga attccagcgc ataaccacca aaacctccag ttgctcctgt tactaaataa
    14101 tgcatatttg ccactcccta ttctttttat ttgttaagat ttacagctgt acatttataa
    14161 attacacctt tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg
    14221 acaactgatg aagtattctc tgcaatttag tgatgcaatc catgtacttg cctatattga
    14281 aatgtttcaa gatacccgat cactttctag tgagatgatt gccagtagta tcgaaacaaa
    14341 tgcagcaaat gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga tctttacaaa
    14401 aaccggaaaa ccgcagcctt cccttgccaa aagtccagat cagatttctt tactagatgt
    14461 ctatcgcagc atcgaaggta atacacagct tatccaagtc gatccaaaaa caaatccaaa
    14521 ttgcgtcgtt ggtgcgaata tccaagaagc tttagaagaa agctacgctc ggctgcagaa
    14581 aaaggcagaa gaagaaatgg ctaagatcac acttgatagt ctgatacata aaatagccgt
    14641 tttggaaaaa gaaaaaagac cagaaaacac aggcctcgta gagagatttt tataattctc
    14701 tctactgtgc ctgcattttt ctgatcggaa tcgtctacat aaattctttt attctccggg
    14761 ggacaaaagc cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt
    14821 catgacgtct tgataaacgg tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg
    14881 caaaatatgg aaagctattt cccatttttc ctctctaatg ctcaggtcaa agcgctactt
    14941 tcacgacctc tatattatca tggtttattg actaccggta tccagatttc tcccatatat
    15001 ttcccgttgg taactcccat ttcgactaca gcatttggac ccccaacata ggcgtaatct
    15061 tctacttctc ttaaaacatg cccaaaggcc gtattcgtca aatcatttct taattcttca
    15121 ggcgattctg ataaagcgct tacaacaaga tacgttcctt ttgggaatgg gatgattcgc
    15181 gtagtgtttg gaacatcctg actcgtttga acaccgacat agtacatcat tttcccgtta
    15241 tagggttcgt tgactgtaaa gaaataatga ttttcagcag cttgtttcaa tgcatctatt
    15301 gttccattac cgataacttt ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa
    15361 tccgtattgc tactcttcaa ttccacacca atacccgtca ccgtaaaatc tggtttttct
    15421 tcgatcctat actttgccat ttctcccacc ctttctatta tattaagttc atcttacata
    15481 aaattaaagg gaaatgacac gattacgctc ttttttctag ggatattttt attttttaac
    15541 tatttttttg ctttcactgc aatccaaatc tcactgtaaa cattactgaa atcctctccc
    15601 cattttgtaa aagagatctc gggagcttcg actaactcat aatctgaaga aggaagccat
    15661 tctgcataga ttttccccca agtatcttgt aaagtttggg gaaatggccc tttattagga
    15721 aaaatggccc aagtgtgttc ttctatagca agcatttcta aatcagcgta cggattttct
    15781 tttgtcgtag caaagccaat catgtgcatc aaactgccct tttcttccaa gcggtctcca
    15841 tcaaatccat aagaaacgtt gattacttga tttggatata aatctcctaa cttatgcatc
    15901 tcttcccttt gagtctccgt gatcgtctgc gctaactttt gtatttcttg attgactcct
    15961 tcaaatttga caggaacttt tttcgccaca cctgccacat aaaactgttc tttcttttcg
    16021 atacgaaatt ccatcgattt tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa
    16081 gatttttgta tgccgtatct gctgatttct gaaggcagat atcctgacca atcacgaaaa
    16141 gctcttgaaa aaccttcgac tgtttcatat ccatatctaa acgccgtttc tgtcactctt
    16201 gatccatcta gaagatcgcg attagccaaa gccaatctac ggtttttgat atagtccatt
    16261 agcgacatcc ctgcaataaa cgtaaagatg cgttttaatt gatattcaga gattcctgta
    16321 ttttcggcta ttattttata ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc
    16381 aattggttca gatctttaag catatttatc ttcctccttg cttgcctttt tatcatatta
    16441 tcaaagaaag tcaattactt gatgaatcag gaaccaattt agtcagatgt agaaaatgtt
    16501 gtatggatct aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg tattatctgc
    16561 ctatctatgt tttaccttta gaaaaataaa ttttggggaa gacagattct aagtaaacaa
    16621 agcggtaaaa atatctttta ttcccttagt taatccgctt actacctact gtacaaagca
    16681 aattgttgtt caaactgctg atcagaacgc tgcttggatt tttctttgcg atacatcata
    16741 cctcttttat caattggaac aattatttat ttctaacaaa atcacttcat ttttgtcaga
    16801 taaataaaaa acaaaaaaag ttccatcttt aaaacacgct attcttttac tttttctcac
    16861 attctctaag aaaagaaaag gagaatgaat ttttgacaat tcgagcaaaa gtgattatca
    16921 aatatgtaaa gtcagctact tttttcttaa acgtttcttt atactaaata taaataagtg
    16981 tttcatgaaa ggagaactag atgaataatt ggcttgagta ttttcctgaa aacgttttgg
    17041 aacgcggtta cagctatcat ctgcacggtt ttgtccgtca tttgaactat acaagcaagt
    17101 acttgtccgc aacagtttct ggcacggaag attataaggt agtgatcact tgggatgaaa
    17161 agaccaacat gacttgtgat tgtctatatg ccatagaagg aaaaaaatgc aaacacatgg
    17221 ctgctgtctt attcgcttac gaagaacgtc caataaaaaa atccaactac tcactttccg
    17281 aactctcgtc ccttgtttcc tccgctagtt cctctcttgt acgggagctt ttaacagaga
    17341 ttcttataga acatccgcac tttatcgaac gcttcaaagt taagatgcca tttcatgcaa
    17401 taaattattc agataaatta acaactatca tccataaata cgatcatatt atcaagaaaa
    17461 acaaaaacag gaaaacagcg aagttcatca tggagatgcg aaagttcatc caagaagcag
    17521 ttgaatcgct tattcagcaa aatgcctatc ttcccgcatt cgaattgatc aatgaagtga
    17581 ttgctactct cgaaacattc tattgggagc ctgaggatga acgaaccctt cttctgatag
    17641 aggactgtta ttatttatgg aaagaattat tagcagaagc acctcatgca gaaaaaaggc
    17701 aaatgttctc ttggttcgtc tgccaagtcg atcatactga cgcctcttat tcaaaacgct
    17761 actccataaa aatcctgaaa gaagactttc gggaaaaaga attttctaac caaaagaaaa
    17821 aaatagataa aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt
    17881 aaacgataac aaccatccta aatccacaag aaatacagca gaccttgctt taaaaacacg
    17941 aatcatgcaa aggatgaagt aaagaagact taacgcgtac ccaatattag tcatttgact
    18001 attaggtacg cgttttgttt tataagtcat gacaaatttc cttcgttgct ggatagattt
    18061 tccggcattg ttgatagatt ttatgatact tttctacatt ttgtaagaga ggttccactg
    18121 catctgtata ttggataaaa ttctgacaac aactttcagg tgtgtcgaac caccctaacc
    18181 ctagtgcagc aatcattgtt gcgcctaatg caggtccttc ctccacactt aaacaataga
    18241 ccgttgcatc gaaaatatca gcttggattt gtagccatgc aagatttttc gcgcctcctc
    18301 ccaccgaaac aatacgtgta aatctgcgac ccgctagttc tttacgtttc aatcccacca
    18361 ttgacaaatg ctcattaaaa atttagaata ttcacaagtt taacgacaat tatgaaaatt
    18421 tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca
    18481 aagacacgtt acctaccgtt ttcggttata tcggtattgg acttgcattt ggtatcgttg
    18541 ggaaagctgc cggatttcat ccactagtcg tcacgttgat gtccctgctg gtctatgctg
    18601 gttctgccca atttatcaca gtcagcatgc ttgctagtca cagcccattg ctttccatcg
    18661 ttttctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt
    18721 atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa
    18781 gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga
    18841 tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac
    18901 ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta
    18961 tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac
    19021 agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa
    19081 gcaacttagt gattgtagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac
    19141 atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt
    19201 tctagggtcc taccatttgt cttactgaaa aaattcgatt taccgcaacc gttgcttgag
    19261 tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc
    19321 caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca
    19381 cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggcagg aattctttcc
    19441 ctttcgttga ttcgcttatt ata
//
LOCUS       DACCSK010000048        19450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCSK010000048 DACCSK010000000
VERSION     DACCSK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.049"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            131..3658
                     /locus_tag="IVP39_001494"
     CDS             131..3658
                     /locus_tag="IVP39_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7586572.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="IVP39_001495"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="IVP39_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7586573.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="IVP39_001496"
     CDS             7446..7787
                     /locus_tag="IVP39_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7586574.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="IVP39_001497"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="IVP39_001497"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7586575.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="IVP39_001498"
     CDS             9144..11564
                     /locus_tag="IVP39_001498"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7586576.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IVP39_001499"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IVP39_001499"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7586577.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="IVP39_001500"
     CDS             12998..13735
                     /locus_tag="IVP39_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7586578.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="IVP39_001501"
     CDS             13749..14564
                     /locus_tag="IVP39_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7586579.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="IVP39_001502"
     CDS             14577..15224
                     /locus_tag="IVP39_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7586580.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="IVP39_001503"
     CDS             15240..15896
                     /locus_tag="IVP39_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7586581.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="IVP39_001504"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="IVP39_001504"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7586582.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="IVP39_001505"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="IVP39_001505"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7586583.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="IVP39_001506"
     CDS             18238..18756
                     /locus_tag="IVP39_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7586584.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="IVP39_001507"
     CDS             18863..19363
                     /locus_tag="IVP39_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7586585.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5395 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca
//
LOCUS       DACCSK010000049        19411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCSK010000049 DACCSK010000000
VERSION     DACCSK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.050"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IVP39_001508"
     CDS             168..362
                     /locus_tag="IVP39_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586586.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IVP39_001509"
     CDS             631..930
                     /locus_tag="IVP39_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586587.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IVP39_001510"
     CDS             complement(1027..1461)
                     /locus_tag="IVP39_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7586588.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IVP39_001511"
     CDS             complement(1693..2403)
                     /locus_tag="IVP39_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7586589.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IVP39_001512"
     CDS             complement(2393..3253)
                     /locus_tag="IVP39_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7586590.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IVP39_001513"
     CDS             complement(3258..3896)
                     /locus_tag="IVP39_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7586591.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IVP39_001514"
     CDS             complement(3911..4210)
                     /locus_tag="IVP39_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7586592.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IVP39_001515"
     CDS             complement(4253..5737)
                     /locus_tag="IVP39_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7586593.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IVP39_001516"
     CDS             complement(5757..6218)
                     /locus_tag="IVP39_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7586594.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVP39_001517"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVP39_001517"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7586595.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IVP39_001518"
     CDS             7521..8291
                     /locus_tag="IVP39_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7586596.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IVP39_001519"
     CDS             complement(8564..9391)
                     /locus_tag="IVP39_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7586597.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IVP39_001520"
     CDS             9959..11194
                     /locus_tag="IVP39_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7586598.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IVP39_001521"
     CDS             complement(12193..12453)
                     /locus_tag="IVP39_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7586599.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IVP39_001522"
     CDS             complement(12480..13781)
                     /locus_tag="IVP39_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7586600.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IVP39_001523"
     CDS             complement(14073..14531)
                     /locus_tag="IVP39_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7586601.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EETLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IVP39_001524"
     CDS             complement(14544..15869)
                     /locus_tag="IVP39_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7586602.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IVP39_001525"
     CDS             complement(15886..17298)
                     /locus_tag="IVP39_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7586603.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IVP39_001526"
     CDS             complement(17315..18079)
                     /locus_tag="IVP39_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7586604.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IVP39_001527"
     CDS             complement(18183..19058)
                     /locus_tag="IVP39_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7586605.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IVP39_001528"
     tRNA            complement(19313..19397)
                     /locus_tag="IVP39_001528"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
BASE COUNT         6178 a         3992 c         2985 g         6256 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tactctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 tcttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata a
//
LOCUS       DACCSK010000050        18680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCSK010000050 DACCSK010000000
VERSION     DACCSK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.051"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..944
                     /locus_tag="IVP39_001529"
     CDS             <1..944
                     /locus_tag="IVP39_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7586606.1"
                     /translation="TIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKTGQTELQVGIQ
                     AVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            1005..2465
                     /locus_tag="IVP39_001530"
     CDS             1005..2465
                     /locus_tag="IVP39_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7586607.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2476..4680
                     /gene="topB"
                     /locus_tag="IVP39_001531"
     CDS             2476..4680
                     /gene="topB"
                     /locus_tag="IVP39_001531"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7586608.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESYRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            4926..5522
                     /locus_tag="IVP39_001532"
     CDS             4926..5522
                     /locus_tag="IVP39_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7586609.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            5535..6434
                     /locus_tag="IVP39_001533"
     CDS             5535..6434
                     /locus_tag="IVP39_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7586610.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            6437..6922
                     /gene="ssb"
                     /locus_tag="IVP39_001534"
     CDS             6437..6922
                     /gene="ssb"
                     /locus_tag="IVP39_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7586611.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            7288..7548
                     /locus_tag="IVP39_001535"
     CDS             7288..7548
                     /locus_tag="IVP39_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586612.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            7995..8201
                     /locus_tag="IVP39_001536"
     CDS             7995..8201
                     /locus_tag="IVP39_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7586613.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            8201..8425
                     /locus_tag="IVP39_001537"
     CDS             8201..8425
                     /locus_tag="IVP39_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586614.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            8440..8877
                     /locus_tag="IVP39_001538"
     CDS             8440..8877
                     /locus_tag="IVP39_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586615.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            8870..9577
                     /locus_tag="IVP39_001539"
     CDS             8870..9577
                     /locus_tag="IVP39_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586616.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            9958..10137
                     /locus_tag="IVP39_001540"
     CDS             9958..10137
                     /locus_tag="IVP39_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586617.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            10230..10415
                     /locus_tag="IVP39_001541"
     CDS             10230..10415
                     /locus_tag="IVP39_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAP7586618.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            10449..10880
                     /locus_tag="IVP39_001542"
     CDS             10449..10880
                     /locus_tag="IVP39_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP7586619.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            11817..13091
                     /locus_tag="IVP39_001543"
     CDS             11817..13091
                     /locus_tag="IVP39_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7586620.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            13145..14572
                     /locus_tag="IVP39_001544"
     CDS             13145..14572
                     /locus_tag="IVP39_001544"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7586621.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            14585..15457
                     /locus_tag="IVP39_001545"
     CDS             14585..15457
                     /locus_tag="IVP39_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7586622.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            15552..16547
                     /locus_tag="IVP39_001546"
     CDS             15552..16547
                     /locus_tag="IVP39_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7586623.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(17152..17259)
                     /locus_tag="IVP39_001547"
                     /pseudo
     CDS             complement(17152..17259)
                     /locus_tag="IVP39_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            17375..>18680
                     /locus_tag="IVP39_001548"
     CDS             17375..>18680
                     /locus_tag="IVP39_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7586624.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTEIEPHLQNSLNILLVSKDSIRKKFNNDAKKFLEENLKKIAVRNFKV
                     SENNFKNLSFTISDSEISELTGDIFNYEIKRYSISGIVITIQNVGEKLKISFEPTDSF
                     QYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPAERTGINVFKNELNESRLKTYDTL
                     LNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTDNSSYPKPISDYINYLNNMKNRYE
                     QKNVNNIASYIRDEIIMGKYEIDEKDNSVSFRQKFGKNRYRKERIPFHVVSSSIKSLY
                     GLDYYLENSSKEGDYLIIDEPELSLHPENQVKLAKVITDIVESKIKLITSTHSDLFVR
                     ELTNIVIENKLNSKNNKKNKLSDKNIGIYNFVGNNDINELEKI"
BASE COUNT         6746 a         2899 c         3693 g         5342 t
ORIGIN      
        1 acacgattga aaaagtgttt gatatcacac aaacacaatt gaagcctgaa gactatccga
       61 aaatcttccc gaaccgcgtc tttgattttc aattggataa aacagggcag acagaacttc
      121 aagttgggat tcaagcggta gccaaaaata ttggaattca aattcgagat atgacagaaa
      181 gtgaagtcta tcaaagagaa ttaggacaag cacgtggggc gtacgttcgt aacgaattta
      241 ctggcgaaga agaaatcgtc atgaatactc gaaatactcc tacgcagcat ttagctacaa
      301 gtattcatga attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta
      361 aagaattgca ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacacgt
      421 cggagaaagc tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata
      481 aggaagctgc ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca
      541 ttcaaaccat ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag
      601 cagaagtgaa gcaggagcat aaaaatactg tagaaacggc agaacaaaag aatattgttc
      661 ctgttggttc actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc
      721 gaacgttaca tttgaaggat gcggcgttta ttgaagatcc gcagcataat caagtgattt
      781 taaaaggaaa aagttatgga aaagagattc aaacggcgtt accttcagaa gaattttcgc
      841 taggaaatcc attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg
      901 aaatctatta tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac
      961 tatctgaaaa acggatagtc ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa
     1021 tgagtcttat tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa
     1081 tggctgtagt caataaagga cgagactatc gcttagaaga ccatgattca tttgtttttg
     1141 atcgaagaaa acaacgtttt tattggaaca gtcaaaatat tagtggcgac attattgaat
     1201 tagctaaact ttttttcata gacaaagaga tccaagactc taagcaacaa tttaaagcag
     1261 ccacagattt tattctaaag aatgaagaca aaacagaacg agtagaaaat cttcattttg
     1321 agacagaaaa gtacaaagat caccccgttg attatcaacc actcacagaa aaaggaagga
     1381 attatctgaa ggaagaacga aaactaccag agtggctgat tgattatgca gaaaaagaag
     1441 ggttgattgc cgaactaaag ccaaaacatg aacggcaaaa tttcttagtt cgagacgatc
     1501 gtttagatca tgcggtcgcc tttttatgga aagatccaca gacaaatgag acagtagggg
     1561 cgagttatca aggaacgttc attgattatg aacgttttgg tgagagaggc acatataagc
     1621 atatagataa aaattcgaca gcgaatcatg gctttaacct aaagatcggt gaccctaagc
     1681 agttaaaatt ttttgagagt agtattgatt tacttagcta tgctgcactg aatcgtgatc
     1741 agttaaacga tacgtggtta gtttcgatgg aagggttaaa acaccatgtg atcagtcatt
     1801 attttggtga ggcagtatct gaattgagaa agaaacaagc ctttcctcaa tccatcgaga
     1861 tttgtgtgga taatgaccgt gcaggacata ttttctacga aaaagaacaa ttaatgggcg
     1921 cagttgatcc atttacgaat cagaaagtac gttgtgaacg aggaatcgct aatgattggc
     1981 aagtgccaaa agaatacaaa gtcatttatg aagaagtcgc aaaagaagaa aagatcacac
     2041 cagaagctat tatggccata cacaagacag aaaacaatct gcaattaacg aaccaattgg
     2101 tttccgctca caaagtaaat gcgtcttttg gtcagcagct ttctgttaac gattcaatag
     2161 aagctattaa tttgaaagat atttgtagag aagtcgcaaa agaattaaaa gcttgtgaac
     2221 gctcgaatgg tacgtacgat tttgatcgtt tttatcaagg aaaaggggat attaatgctc
     2281 agattctttt ctcttataaa gcagaacaat actacaaagg ctataaaaag catgaacatg
     2341 aatttgtacc tgaagtgaaa aaggattgga acgatcaatt gaagcatgag atccagcaac
     2401 aagaaattag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg
     2461 agtgagatcg tcacgatgaa aactgtcatt ttagcagaaa aaccgtcaca agcgaaggct
     2521 tatgccgata gcttttctaa agccacccga aaagacgggt attttgagat ccaagatcga
     2581 ttgtttcctg gtgaaactgt gattacgtat ggttttggtc atttagtcga gttagattcg
     2641 cccgatatgt acgatgaaaa ttggaaacaa tggtcattag aacatttacc tattttccct
     2701 acacattatc actatcatgt tcctaaagat aagaaaaagc aattcaacgt ggtgaaacag
     2761 caacttcaat cggccgacac cattattatt gcgacagata gcgatcgcga aggggaactg
     2821 atcgcttgga cgatcattca acaagcaggc gccgatcatg gaaagatatt taaaagactt
     2881 tggatcaact ccttagaaaa agaagccatt tatcagggat tccagcagtt acgagatgca
     2941 gaagagacct acccaaaatt tgaagaagca caagcacgcc agatcgccga ttggttgatc
     3001 ggcatgaacg gtagcccgct gtatagttta ctcttgcagc aaaaaggcat accaggaagt
     3061 ttttccttag gcagagtcca aaccccaacg ctttacatga tttatcaatt acaggaaaag
     3121 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
     3181 ggcgcatttg atgcgaagct tgatccgaat gaaacccaag cgacgcaaga agcatttgaa
     3241 gattatttaa aagaaaaggg ggttcaattg ggaaaacagc caggtacgat ccaccaagtt
     3301 gaaaccgaga aaaaaagtgc ggcaagtcca cgtctttttt ctctatccag ccttcaatct
     3361 aaaatgaatc agctcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
     3421 tatgaaggga aatatctaag ctacccacga acagataccc cttatattac agaaggggag
     3481 tatgcgtatc tgttggatca cttagacgaa tacaaacact ttttaaaggc cgaagccatt
     3541 cctactccta ttcatacacc gaatagtcga tatgtgaata ataagaaagt ccaggaacat
     3601 tacgcgatca ttcctacaaa gacggtcatg acggcagcgg cttttgaaca attaagtcca
     3661 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     3721 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagct aaagacagtc
     3781 ggaaaagtac cagtagatct aggttggaaa aagctgtttg gcaaagagag ctacagaaaa
     3841 gaaaaggaag aagaaccgct acttcctaaa gtgacaaaag gagaagcggt gacggtagat
     3901 ctacaagtcc ttgaaaaaga aacgaagcca ccacaacctt ataccgaagg tacgttgatc
     3961 acagcgatga aaacagctgg aaaaaccgtg gatagcgaag aagcacaatc catattgaaa
     4021 gaagtagaag gaatcggtac agaagcaaca cgagcaaata ttattgaaac tttgaaacaa
     4081 aaggaatata tcaaggtaga aaaaaataag cttgtggtga caaataaagg gattttactt
     4141 tgccaagccg tggaaaaaga gccgctgcta acaagtgccg aaatgaccgc caaatgggaa
     4201 agttacttat taaagattgg tgagcagaaa ggaacacaag ctactttctt agctaatatc
     4261 caaaaatttg tctctcactt gttggaagtc gtaccaggac aaatccaatc cactgatttt
     4321 ggatcgacgt tacaagaggt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
     4381 gggatttgtc caaaatgcca ggaacaagaa gttttactct atcataaggc ggccgcatgt
     4441 acgtctgaag cttgtgattt tagattatgg acaacgattg ccaaaaagaa actcacagcg
     4501 acacagctga aggaaatcat tcaaaatggc cgaacaagcc agccagtgaa aggattaaaa
     4561 ggtcaaaagg gttcgtttga agcaacgatt gttctaaaag aagacttcac aacgagcttt
     4621 gaattttctg aaaagaaaaa aacgaactat aagaaaagaa cacgccgcac tacaaaataa
     4681 tggaggggtt atcttatgcg aaatacaaca cacaaacaac aagaaatcga aaatcaaaaa
     4741 gcaaaaattc gttcagaaca agagaaatta cgtgccagta aaagaagact tcagcaatta
     4801 aagaaccagc aacggattca aaaagaaatt gaattagatc gataaaatct tcctggctat
     4861 tgtatacgca taaacttatg ttatactata tttataagtt tatacgtata caaaggaggg
     4921 agaaaatgac aaaagcaatg gtttacaagc tgctagaaga atttaacgga actttggctt
     4981 tgaaggatgc caaaaaagcg ggcatttctc ctgtaacgat caaacgtatg gtagatcgtg
     5041 gagagctgga tcgtgagtat ccaggttttt tcaccttacc tggtcaattt cctgatgaat
     5101 tgtttatggc acaaaagaaa tatgagcgcg gcattatttc gcatatcacc gcgttggatt
     5161 tatatgattt aacggatatg atccctagac agatcgatct aaccgtacct tacggttatc
     5221 acgtttctga aaaggggttg aaagaattcg cagttgagtt acactattca aaagcggaat
     5281 ggtatgaatt aggtaagatc gaaataaaga gtcgttatgg gaatcctgtt atagcttatg
     5341 atccagaaag aaccttgtgt gacatttgga atccctggta caacgttgag gatgaaatca
     5401 aagtaaaagc aatcaaaaac tacatggagt cggacagaaa aaatcttaga aaattgaatg
     5461 agtatcgacg aattttgcct acagacaaaa cgatacgctc ttatattatg gctttaaatt
     5521 aaaggagaag acacatgaca cctacgcaat taaaagcaaa agtgaagaat atttccagag
     5581 aaaaagaagt tgatccgcag cttgttttaa gacattttat gatggaaaaa ttcttggaaa
     5641 aaatctcaga gtctccttat cgtgaaaatt ttgtgttaaa aggcggcttc ttaattggct
     5701 caaagtatgg cattgaaaat cgaacaacaa aagacattga tacgacttta cgggaaatga
     5761 aagtaaccaa agaaacattg acaacggttc taaacgatat tttttcaacg ccgacgaaag
     5821 aaggcattca atttgagatt caaggaatga aagaaacaag ggaagctgat tattatccag
     5881 ggtttagctt acgtgtactg gcacatttgg aaaacatgcg gccagatttt aaggtagacg
     5941 tgacgacagg tgactcgatc tatcctgcaa cgatcactca cagtcataaa ttgatgtttg
     6001 aaaatcgaac gattcctttg gaatcttatc ctacagaaca aattattgcc gaaaaattaa
     6061 gtgcaacctt tgatttttta acagataatt cgagaggaaa agatttttat gatctctata
     6121 ctattcctaa aatggaaaag atcgatgaga agaccttgaa agatagtgtg agaaatacat
     6181 ttacacgtcg aggaaaaact gatccgttaa agatttatta cgaaaaggat atggatgtac
     6241 tgaaaaatag tcatgagcta aaagatacct ggaagaaata tcaaaaaagc aatccttatg
     6301 ctgcgtcgat cacgtacgaa gagacaatgg attcgattag taatttgatg gaaaaagtga
     6361 ttcacgtcga agaaaaagag cgaaaaatag cttatcaaag acaccaacag atgaacagag
     6421 aaatggagag ataaaaatga tcaataatgt aactttagta ggacggttaa ccaaagatcc
     6481 agatttacgt tataccgcaa gtggcacagc agtggctact tttaccctag ccgttaatcg
     6541 taatttcacg aatcaaaacg gcaatcgaga agctgatttt atcaattgcg tgatttggcg
     6601 caaaccagca gaaacgatgg cgacattagc taaaaaaggc atcttgatcg gcgttgtcgg
     6661 acggattcaa acacggactt atgacaacca gcaaggacaa cgtgtgtatg tgacagaagt
     6721 tgtagctgat aacttccaac tattagaaag caaagcagcg acagagagcc gtgcgcacgc
     6781 tgatcaatcg tccacgagtc caagcaccac cacttttgaa caacgggata cagcgacgcc
     6841 taacaacaat ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag
     6901 cgatgatgat ctaccgtttt aagtagcatt ttctattggt tgcatttgct acggtttatg
     6961 ttataattta gtcaacaaaa agaattttca atctataacc aaaaaccact agctggcggg
     7021 ctagtggttt tcttttacgt ttctattata agaattaacg agtaggctta ctgtctttct
     7081 tggcaaattt acaatcaagc catttgcaga tatagtgcgc ggctacttct gccatgattg
     7141 caaaaacaaa atcaaaaaga attttcaaac ctataacctc ctccccttgc cagattaggc
     7201 cggggtagca gacagcaaga ctattatagc ataattgaaa tatccaaatg attggatttt
     7261 tgctataatt ttagcgaggt gattgtgatg aacaaatatg ttggtttgct tgataaaatc
     7321 cgagtaataa aaacgcaacc gttattggtt cgctttactt tacaaacgat tcatgaatcg
     7381 attaattgtg tagttgcaga tattgaaatt atcgataaat tgctaattat ggatgatgga
     7441 aaatacaata ttgccgtcac aggtcacttc aataaacgaa atcagctggt tattgctagc
     7501 atgtatgtta gaaatcccga tcattttaca cgatcgatgg gcatataaat aaaagaagag
     7561 ttgtagtcct aaatgaggat tgcagctctt tttgttttac atagaaacgg gaacaggaaa
     7621 aggcctgttt aggaaggcac aagcgccttc tcaattaaaa aacctttctc tcactccaac
     7681 cgccaccgcc gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc
     7741 ttgcaaagca agacaaaata ggaaaagctt cctctcacca aaaggttcgt tcctgttcgc
     7801 tatctcttta ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca
     7861 aggacaaggg taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgattcatt
     7921 ctggccaaca aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg
     7981 aggcagaatc aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat
     8041 tcccaaacta tcatgcacca catactgtcg aatgcgattg tggagaaaga gcgaaatgta
     8101 tcaaaagcta tcgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg
     8161 gcgattacat tttgattgat gagaaaggaa agaaacgctg atgaaaacac aacacaaatt
     8221 attgaatgta aaagaagtca tttctgaaaa agtagcagaa gctttactta ttccgcggta
     8281 ctggacgata gaaacccctt tagtagagga cgcaccgact atgctatttg aagacctagt
     8341 gaagattgca ggatatgaac tgcattatca agaaaacttg gaaacttatg aaaacgaata
     8401 ttggttaacc aaattagaga actaaaaagg agaaaaaata tggctcagaa ttactatgac
     8461 gaatttgtca aattaccttt ggataaaatg gcacaaaaaa tggaggatat gacgttccta
     8521 taccatgaaa cacgtgtacc caaaaaacat tacaaggaaa aattgtcagt cgcagtggaa
     8581 gaaatgattg aatcaggtgt cgaaatgaat ctcattgcga cgtactatcg aacattggaa
     8641 gaactgaaaa aacaaaatgg aaaatggttt ttccaagcct tactatgctt ggaagcaggc
     8701 gtcaaaccaa gcacaatcaa accttctgaa taccaagctt tagaactaac gtatggaaaa
     8761 tttatcgaaa cgaaaaaagc caagactgtt tcaagtgaat ggttggacta ctttgaaaat
     8821 gtcaacaagt atggcgcatg ttacatcatg aaaaaggagg acaacgaaaa tgagtaatgt
     8881 ggtttatcaa ctaacagtgg aagatgtttc tcacttttta acaaacaaag aacatttgaa
     8941 tgaagtagtc agacggattc aaaaagactt tagttgtatg gaagaaatga agtactttat
     9001 tcaaagagtg gaagaacagt tgtatatgga aagcgaaacg agtcaaacga aggtcacagt
     9061 cagccataat catttagacg tgtataaacg tcgtcctgaa acggtaaaaa aaggaacaga
     9121 gcttctacga ctagaaacag aaaaatacat catgaatgtg tacctagaaa ggagtgagct
     9181 ggtcgaaaac tttgtcagct acacgatttg taggaaacac tattactcag aatcagaata
     9241 ttattcaatt tttgagagca tggaattaat ggaagaagaa atggaagagc taaacagcaa
     9301 aaatgcgacg gtagacgatt attaccttgt atttactaga tttctgacgt gtgggacgga
     9361 atctatcgtg ttaatgggaa tggattatga actcgctgta cccgaagcat ttttgaggga
     9421 atatgtagaa gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt
     9481 agatacctac acttacgacg atacagaaga aatccaaaat gagcttgact gtaaacgaat
     9541 gccatatcaa cgatttgaaa ggaaaaaagg tcaataacca tttataccga aaagtgagaa
     9601 tgtgtgaaat aagaacaaaa aaccttgaaa ttaagggaat ggttcttatt tcgcaccaat
     9661 cgctccaacc gcccccgccg ctcaggtggt gcgaaataag cgggcaacga ccgataacct
     9721 aagagaaaga aggaaatcca tatgaataaa atcattcctc tgttgttcgt actgtttgtt
     9781 ggcgagatac ttatgcgtca tgctgcagct gtacatttaa acttcgtagt attcttagga
     9841 atactggtat tttgtttgat cttttggcga tttgtattag cccttgtgag tctactattc
     9901 aagatgattg cagccatatt tttcataggg ttatttcgat gaaaggagag agtactaatg
     9961 ccaattgcaa taggaaataa acgtttaccc gtgacgttgg atgaaaagag acaaaaagaa
    10021 ttgcaggaac tcaaacagaa gtacggcaaa agtgaatcca ggattatgtg tattgcgtta
    10081 gatttattga ttgcacaaga aaaagcaggg tttgatgtac cagcactcaa aaagtgacgt
    10141 cacttttcat ttgaagaaac aaaagtgata gcactttacg cacgtgtcga tacgtgttat
    10201 gattattttg taaggaggta tagtgcacaa tgccacttac gggaaaagaa atgcttaagt
    10261 tattgaaaaa aaatggctgg gttgagcgaa ggcaagaggg atctcatcat catctttata
    10321 aggatggtgt tagaatcact gttcctgttc atgccaatca agatcttggc cgaggacttg
    10381 agcaaaaaat tttaaaggat gcgggattga aataatctcg cattcgatta aagaaaggat
    10441 tgactgaaat gttaagtgag cctttagcaa aaacctatcc tgctattttt agcccagaag
    10501 aaggtggtgg gtactttatt gaatttcctg atgttcaagg tgcttacaca ggaattaacg
    10561 aagacgatat atcttatgga attgctatgg cacaagaggt gttaggtatg gttttagcag
    10621 attatattga gcatgaagat ctacttccag aaccaacacc aatcaataag atttctgttg
    10681 aagatgattc ttttactact ttaattcgtg tagatgttgc caaatatttg aaagacacag
    10741 aacttgtcaa aaaaacctta acaattcctc aatgggcgga taagctaggc aaaagggctg
    10801 gcataaattt ttcagtcctt ctaacagaat caattgcaga taaagcagat aacattcttc
    10861 gttctggaag aaataattag aactcaaaag agtcagaaac ttaaaaatct ttggcttctt
    10921 tttttactac aaagatggct tctttaaatt atttgcctga ttgatttaaa aaaattgcgc
    10981 aaagactacg agctaattaa gttgctcgtg gtctttcttt atgaaaactt tttagatcta
    11041 gataaaaaca ttgagacact ttgaaaaatt tttttaatag taatgggcgc ttctatgtca
    11101 agctgattct tctttcggta aattaatttt acttagaata tcactatctt gataaaaaat
    11161 ggtgtattca caaattctgg cataaaaaat acctactttc tagttaaggt tcctgaataa
    11221 gtcgcaatcc aattatttcc agctgattta taacttttta aatataattt tccataatac
    11281 caataaccat tacttttttc tgtccaattt cgttattgag aagatctaga aggccagcca
    11341 actttatcaa aaatcatcat tcctgtcact cgtatcgttt tagaagctgc tcgagtaagt
    11401 aatggagaaa ctgttaaata attttaagta acagttaagt cgttagttgg gttatcaatg
    11461 gctattccgt ccttatctga acctcgaaac ccaatcatag aaaaagtttt ttgaacaagt
    11521 tttaacagat aaatcaagtt atatactaga aaattcgtaa aatatatttt taatgtcata
    11581 atcgtttgat ttttctggat tgacatttat ttataaaaaa tagtgccatt aaaaaagaaa
    11641 agtgacacac caataaaagt catatcctgt gacattatat ggcattatcc aaatagaata
    11701 accggttgac atgtaagcgg attcctttta taattttagg agtataagtc cttttctaaa
    11761 aatattaacc ggttatcatg aaaggggata caaatatatc gttggaggat aaaaatatgt
    11821 caaaggttaa cgtatttaaa aatcaatcct atttacaaag ttcagctaca ttattactat
    11881 tttttgcttc ttggggtgtt tggtggtcat tttttcaact ttggctaaca tctgaatcaa
    11941 atggtttagg gttatctggc agtgctgtag gaacagtatt ctcggcaaat tcgttagtta
    12001 ccttaatttt gatgtttatt tatggaacat tacaagataa attgtatatt aaacgaaatt
    12061 tattaatttt tgcttctgta ttagcgacac ttgttggacc attttttata tggatatacg
    12121 ggccattgct agataacaat tttaatttag gcattattat gggagcacta tttttgtcag
    12181 ctggatattt agcttctgta ggagtttttg aagctgtgtc agaaaggttt agtcgtttat
    12241 ttggctttga atatggacaa gcaagggcgt ggggatcatt tggttatgcc ttggtagcgc
    12301 ttttggcagg atttttattt gtaaaaaatc ctcatttaaa cttttgggcg ggatctttct
    12361 ttggttcttt actattgtta aatttattat tttggaaccc taaagttgaa cgggaagcaa
    12421 atcaaaattt taatcaagaa caagctgaat caaatagtat tccttcttta aaagaaatgt
    12481 ttgatctaat gaaactgcct caattatgga cgataatcat ctttattgtt tttacatgga
    12541 cattttatac ggtattcgat caacaaatgt ttccgggatt ttatactggt ttgttttcaa
    12601 catcagctaa tggtgaaaaa atatatggga cattgaatgc tattcaagta ttttgtgaag
    12661 cgttaatgat gggaattgtt ccaatcatta tgagaaaatt aggggttcga aatactttgt
    12721 tattaggtgt aaccattatg tgtgtacgaa ttggattgtg cgggtttgcc tcgacaccat
    12781 tatctgtttc atgcataaaa atgttgcatg ctttagaagt accattattt acattaccaa
    12841 tgtttcgcta ttttacactt cattttgata caaagctatc agcaaccctc tatatgatag
    12901 gatttcagat agctgctcaa attgggcaag tgattttatc aacaccattg ggaatattaa
    12961 gagacaacgt tggctatcaa ccaacattta aaattatttc tcttattgta ttactagcag
    13021 gcatatatgc attctttatt cttaaacaag atgatagaga tgttcaaggg gatccattta
    13081 ttcgaggata atggaaacca acagaataaa aaacaatttt atctagaaag tgagcgaata
    13141 tattatgaca aaaagagcta tagaattaac aaatacacgt tatcgtttgg gatatcatgt
    13201 gtctgcacca tcaggatgga ttaatgatcc aaatggattt tgctattttg atgggtatta
    13261 tcatgtgttt tatcaacatc atccatatag tgctgaatgg ggaccaatgc actgggcaca
    13321 tgctagaagt aaggatttag ttcattggga atctttacca cttgctttga ctcctggaga
    13381 tcaagaagat gaaggtggtt gtttttcagg aagcgctatt gaaaaaaatg gtgtgttgta
    13441 tctgttctat acaggacatc actattatgg agacaatgat ccagatcatt tttggcaaaa
    13501 tcaaaacatg gcgtatagta cggatggcat tcactttact aaatatgaaa aaaatcctgt
    13561 aatagcaaaa gcaccagaag ataacacaca tcatttcaga gatccaaaag tatggaaaca
    13621 cgatgaaaaa tattacatgg ttttgggtag tcaagaaaaa gatggattag gtcgtgctat
    13681 tgtttatcag tcgttagatt tattaaattg ggattacatt ggaccaattt ctaaagcgaa
    13741 ggaagtactt acagaaggat ttatgtggga atgcccagac ttttttgaat taaatggaaa
    13801 atctattctt ttattatccc ctcagggaat tgaagcaaat ggaaaagatt atttaaatct
    13861 atatgaaaca ggctacttta tcggtgatta tgattatgag acagcaaaat tcattagagg
    13921 aaatttttat gaattagata agggacacga tttctatgca acacaaacaa ctttatctcc
    13981 agatggtaga cgtattgtta tggcttggat ggatatgtgg gaaagcccta tgcctgaaaa
    14041 agtagacgga tgggcaggag cattaacgct tcctcgagaa ttagagttaa ttggagatca
    14101 tttgtatatg aaacctgtga gagaattaga acaacttcga gttggcagcg gcgttgaaac
    14161 aaaagttaat ttggcaggag aaataataat cgctcaagat gcttcattca aagaagctct
    14221 aattgatgta ctattgacta aatcaaaaaa tcaagaaatc aaattttcat taaaaacaaa
    14281 agatgaagag ttaattaata ttttttattc taaggataaa aatgaatgta ccttaactcg
    14341 taacggcaaa aatgatccaa ggtatggaac cattcagatg tgtgatagat tatcacttcg
    14401 agtgtttatc gataaaagtt cggcagagat ttttattaat gatggcgagc ttgtttttac
    14461 agagcgttat tatacagaaa atcaaccaag tatgcatttg agtgttagtg aagaagcttt
    14521 tgtctcgagt acaatttatc atctaaaaaa agatgcaatt tcttacgagt aaggaggttt
    14581 tatgatgtta attggtagca tagaagcagg tgggactaaa tttgtatgtg ccgtgggaga
    14641 cgaggattac cgagtacaag accaaataat ttgtccaacg accaccccca gggaaacatt
    14701 gaaaaaaact gttgactatt ttaaacagtt tgatataaat gcattaggta ttgcatcatt
    14761 tggtccaata gaaattcgaa aaaattcacc aaaatcagga tatattacgt ctacaccaaa
    14821 ggtgggatgg cgaaatgtta atgtgttagg ttatttacaa gatgagctga atgttccaat
    14881 gtcttggaca acggatgtga acggatcagc ttatggagag tacatcatgt ccactttatc
    14941 gaatgaaaaa ataaattcat tagtttatta caccgttggt actggcgttg gtggtggagc
    15001 tgttgttgac ggaaaactat taggatcgtt tggtcattca gaaatgggcc atacgtttgt
    15061 aaaacgccat cctgatgata ttgattttaa aggaatttgt ccttatcatg gtgattgttt
    15121 agaaggctta gttgcgggac caacgtttga agcacgtttt ggaaaaaagg gtgaagacgt
    15181 ttctttaacg gatcctattt gggatgtaat ggcctattat ctagcacaag ctgtgattca
    15241 aacaacattg attatacgac cggataaaat agtatttggt ggaagcgtag taaatgaaaa
    15301 attgttagaa aaaattcgtg ttaatgttaa tgaattattg gatgattacg tggaaatacc
    15361 accgttaagt aaatatatta cgcgtccttt aattaaaaat aatggatcgg caacactagg
    15421 aaactttgct ttgggcttga atcaattaca agaataaaac atagaaaaaa tatcgattat
    15481 caggtatagt agttctatag agttcttttt ttcgatagtt aattattgca ttatttagga
    15541 gagacaagag aatgcgccca aaattagaag atgttgccaa aagagcaaat gtatcaaaaa
    15601 caactgtttc acgtgtatta aacaatcgtg gctatttaag tcaaaaaacg attgataatg
    15661 tttataaggc tatagaagaa ctcaattatc aaccgaacgt tgtagcgcgt caattatttc
    15721 aaaagaaaac taacattgtc gggttactat ttcctacagt agccaatcct ttttttagtg
    15781 aactcgttga ggcattggaa aaaaagttat atgaaattgg ttataaggta ttaataggca
    15841 attcgatgaa taataaagaa aaggaaacaa attatttaaa tcagttattg tctgatcaag
    15901 tagatggttt aattgttggt acacataacc aagggattca ggaatataaa tatcagaatt
    15961 taccgattgt tgcaattgat cgagtgatga atgaagatat tccagttgtt gaatccgata
    16021 actacaatgg tggaaaactt gcaaccaagt tattaattgc acaaggtgca aaaaatatta
    16081 ttcatacaaa cggtccaatt gacttacaga ctccggctaa tcgtcggaga ctagcctatg
    16141 aagatactat gaaagcatat cagttaattc cacgaactgt gacattagat ttcaatattt
    16201 cttatgtaaa gaaaaaacag atttttttcc aaatgtttga ggattatcct aaaatagatg
    16261 gtatttttgc ttcaaatgac atcgatgcgg ccctaatatt acaagtagca aaagaaaagg
    16321 ggttaaatgt accagctgat ttattagtag tcggatatga tggtacttta atgactcgga
    16381 gtatattacc agatctgaca acagtaatac aaccaattaa tgatatagca gacacagctg
    16441 ttgctatttt gatgaaacga ataaataaag aagaaacaaa aaaggaatat attttacctg
    16501 tcacattatg gatggggaag acatcagtaa agaaaaagga caattaaatt tataaagact
    16561 atcaatgata atttcattat taattcgtga tataaaaagt tgttaaatct cttataatag
    16621 actatgatac aaaatcatgc ttttaatctg tgtagatgtt gccaaatatt tgaaagacat
    16681 agaacttgtc agaaaaacct taacaattcc tcaatgggcg gataagctag gcaaaagggc
    16741 tggcataaac ttttcagtcc ttctaacaga atcaattgca gataaagcag ataacattct
    16801 tctttctgga agaaataatt agaactcaaa agggtcagaa acttaaaagt ttttgatcct
    16861 ttattttcaa gtctgatttg acagattttc ggcagctcat ttatttatgg tcagaaatag
    16921 ttgctgaaat tttaatctat tagcaaatag tccagaacgt atgaagagga ttcggatatc
    16981 atttgtcatt aacaccataa atatttataa aattcgtgtt cacctacaat taaatttaat
    17041 atataatatt cgttgtaagg gcttataatt ttttgttaat gaacaagata tgtaattgat
    17101 agtgtcaata gctattcaaa aatgtaggct tttggcccat aaataacaag gttacttata
    17161 tgataacttt ttttctttat aatctttaat ccgatagagc tttccagtag tttgaataat
    17221 cttagcgtgg tgcactaatc gattgattag tgcatttgtg ttcagtccat tgagacataa
    17281 tagtggatag ttttactgag aaaattgata aaaaatagag atataattca aatacgtata
    17341 taatgaaact aaagataaat acggaggaaa atatatgatt ataaaaaact taggaaatgt
    17401 taaatcaata gatatacacg ttaaagattt gctgattttt actgggaaaa ataatacggg
    17461 aaaaacatat accagctata ttttatatgg aattttatca gaactctttg gagcaagatt
    17521 tttcaattat ttttctcata cagaaattga accacattta caaaatagtt taaatatttt
    17581 attagttagc aaagactcta taaggaaaaa atttaataat gatgcaaaaa aatttttaga
    17641 agaaaattta aaaaaaattg cagttcgtaa ctttaaagta agtgaaaata acttcaaaaa
    17701 tctttctttt actatttcag attcagaaat tagtgagctc actggtgata tctttaatta
    17761 tgagatcaaa agatacagta taagtggcat tgttataaca attcaaaatg taggagagaa
    17821 attaaaaatt agctttgagc caactgattc atttcagtat cttagcaaag atattgaaat
    17881 tgaagatatt tataaaattt tagctcaaga gctatctaaa aatgttatta gtttgccaaa
    17941 ggttttttat tttccagccg aaagaactgg tataaatgtc tttaagaacg aactaaatga
    18001 aagtcgctta aaaacatatg ataccttgct gaatactgtg caatttacta atttaactaa
    18061 ccaaaaaaag aaagctgccc tcaagtacga tctgttaaaa aagaatattg agctgttaat
    18121 ggagacaacg gataatagtt cttatccaaa gccaatatcg gattatatta attatctgaa
    18181 taacatgaaa aatagatatg aacagaaaaa tgtcaataat atagcatcat atattagaga
    18241 tgagattatt atgggtaaat atgaaattga cgaaaaagat aattctgtaa gttttagaca
    18301 aaagtttggt aaaaatagat atagaaaaga acgaattcct tttcacgttg tgtcttcttc
    18361 gattaaatct ctatatggat tagattatta cttagaaaat tcaagtaaag aaggagacta
    18421 tcttattatt gatgagccag aattgagttt acatccagaa aatcaggtta agttagcaaa
    18481 agtaattaca gatatagttg agagtaaaat taagctaatt acttctaccc atagtgattt
    18541 atttgtacgt gagcttacaa atattgttat tgaaaataaa ttaaatagta agaataataa
    18601 aaaaaataaa ttgtctgata aaaatatagg catatataat tttgtaggaa ataatgatat
    18661 aaatgaatta gaaaaaattt
//
LOCUS       DACCSK010000051        18562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCSK010000051 DACCSK010000000
VERSION     DACCSK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.052"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(360..926)
                     /locus_tag="IVP39_001549"
     CDS             complement(360..926)
                     /locus_tag="IVP39_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7586625.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(926..1882)
                     /locus_tag="IVP39_001550"
     CDS             complement(926..1882)
                     /locus_tag="IVP39_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7586626.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1882..2538)
                     /locus_tag="IVP39_001551"
     CDS             complement(1882..2538)
                     /locus_tag="IVP39_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7586627.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2908..4926
                     /locus_tag="IVP39_001552"
     CDS             2908..4926
                     /locus_tag="IVP39_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7586628.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5353..7767
                     /locus_tag="IVP39_001553"
     CDS             5353..7767
                     /locus_tag="IVP39_001553"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7586629.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8115..9668
                     /locus_tag="IVP39_001554"
     CDS             8115..9668
                     /locus_tag="IVP39_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7586630.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10049..11833)
                     /locus_tag="IVP39_001555"
     CDS             complement(10049..11833)
                     /locus_tag="IVP39_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586631.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11848..13569)
                     /locus_tag="IVP39_001556"
     CDS             complement(11848..13569)
                     /locus_tag="IVP39_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586632.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13669..14430
                     /locus_tag="IVP39_001557"
     CDS             13669..14430
                     /locus_tag="IVP39_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7586633.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15397..15665
                     /locus_tag="IVP39_001558"
                     /pseudo
     CDS             15397..15665
                     /locus_tag="IVP39_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16337..>18562
                     /locus_tag="IVP39_001559"
     CDS             16337..>18562
                     /locus_tag="IVP39_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586634.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQ"
BASE COUNT         6101 a         3297 c         3740 g         5424 t
ORIGIN      
        1 aatttgactg atattaagtt agctattgat atatgaatga catacaaaaa tataaaatct
       61 tttaaaaatg cttatatatc aatgtttttg ccattctttt atatatctgt tatatgtcaa
      121 aaaaatgtgt tcaatttcaa tttgtatttg aataaagagg ttgtgaaaaa gctgtcctgc
      181 atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgatt tggttggact
      241 tattaccgca gatataagct tttgagcacc gtgaataaag aggttatgaa atcaacgctc
      301 taacctgagt attagagaaa aaaacgcaaa acatatcatt tatgttttgc gttttttatt
      361 tatctttttt tctcaataca atataaaata ggcggctggt tcttttggtt gataaaccga
      421 taattcagca cattatatgt ttcctgtggc agtttggaac aaaaagaatc aaccatatcc
      481 agttcttttt ctccaccttc atgaccatag tagacgacaa ggatcaatcg tccgcgaggc
      541 acaagtcgtt tcaatatttc ttccatagct gtgcgtgtag tctctggcat agtgatgatt
      601 gctttatcac tttttgggag ataccctaga ttgaaaatcc ctgctttgag attttgttgt
      661 tcagctatca cttctcctaa cgtttcgtgg ccttgtagaa acaatgagac acgttcttct
      721 aactgacgct gttctagcct ttcacgagta gcatcgattg cttgttgctg gatatcaaaa
      781 ctatagacat gaccagtttt tccgatgtgt tctgctagaa aaaccgtatc gtggccattg
      841 cccatcgttg catctacgac gtgatcccct ggttgcaaga tttcttgcag taatgtatgg
      901 ctgaaatgca aagcagtttg caacatcaga cgctcacctt tttccgtaca tcatattttc
      961 cttggaagct atctctgcga agaagttctt catcaattgc attcaagact tcccattttt
     1021 tcaagctcca cattggtcca atcaaggaat cccacggcgc atcccctgtc aaacgatgga
     1081 tgatgatctc ttttggtatc atttccagct ggtcgcaaat gacattgaca tagtcaggac
     1141 ggctcattaa ttgaaggcga ccttcatgat agtcccgcaa cattctcgtt ttgcgcatca
     1201 gatgcatcaa atggagcttg attccttgga tatctgaatc taaaatggtg cgtctgacat
     1261 tttctttcat catttctaaa ctttcgcctg gtaatccgtt gatcaaatgc gtacaaacac
     1321 ggatattatg tttgcgtaat ttagccacgc catctaaata agtttgataa tcatgtgctc
     1381 ggttgatcag ttttgaagtt tcttcaaaag ttgtttgcaa tccaagttct acccataaat
     1441 acatccgctc gttcagttca gcaaggtaat caactacttc atctggcaga caatccggtc
     1501 gtgtaccgac tgataatcct acgacacctg gtaaattgac tacttgctca aatcgttctt
     1561 tgattatttc aactggcgca tgggtgtttg taaaattttg gaaataaacg atgtattgtt
     1621 ggacatgagg ccatttttga tgcatcatgt cgatttcttt ctggaattgg attggaagag
     1681 ggtcttcagg tgcaacgatc atgtctcccg aaccagatac actgcaaaaa gtacaaccgc
     1741 catgggcaac agtcccatct cgattcggac agtcaaatcc tccatcgatc ggcaccttga
     1801 aaattttccc gccaaactca tttcgtaaag cgtaattcca agaatgatat cttttatttc
     1861 cttctgaata aggaaaagtc attaaaagtc cttctttctg taagagttat ctatttgttt
     1921 tcttttttct ctatacactg gataagtcag gcacagccaa gaaagactga gtaagtagcc
     1981 gccgatgata tcagtaggaa aatgaacgcc cagataaatg cggcttatcc cgacacttac
     2041 tataataaac atcatcagca attgaagcaa tgtgcgtaag gttctttttt gaacaaaagc
     2101 tggaagcaga aatagtaaag aaccgtaaag aatcatactg cctgctgcat gtccactagg
     2161 gaaactgtaa cttgtttcaa ctactaagtg cgccaatgaa ggacgctctc gcatgaaaaa
     2221 tagtttgatc accgggttga tgattccagc aattacaata aatccagaaa cgaaccaaaa
     2281 tgtttctgcc ctttttttct tataccacat ccataaagcc gcacaagcaa ataaaatgat
     2341 gacggtaaga ggattaccga atttagtcat aaagagttgg tagctgttca gatcaggata
     2401 agaagctctg acaatgcctg taaaccactg atcgaaacca gaaagccacg acagatggaa
     2461 ctttacgatc cagcccaaaa tgaaaaacag gacaagaaag aagctaccag ccacctgaaa
     2521 tgatgtttta tatttcatac aattcctcct ttgaagcaat gacttattat agcatttttg
     2581 gtccagcgaa aaaaacggta aagagataaa accaatgtat gattcctctt tgaataacca
     2641 atattcttat aaaaaaaata actgtaatcg gaaagacatt ttttggtaat gcaaatgaaa
     2701 tgttagggaa ataaaatcag tgtttttcga aatcctttta aacaagaacg tagacgtagg
     2761 aagaacttga aaatgaacag aagttgttca ttacggtaaa ttacaatatg attacaaaaa
     2821 acaacaaagc tcttaaataa cttttaataa atattaagag aagttataat tattaataat
     2881 agataaattg aagttggagg atagaagatg gaaaacattg caagaaaaga acggagacgt
     2941 ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt gggaactgcg
     3001 atggtaggct gttctgtcgc tgccccatta gtacagccgg ttcctgttca agcggatgaa
     3061 acacctgttc agttcggcgc aagaatcaat acagcggcat ttatcacaga aattgctact
     3121 tatgctcaac caatcgctca agccaacgat ctatatgcat cagtgatgat cgcacaagca
     3181 gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag caccttatta taatcttttt
     3241 gggataaaag gcagctatca aggacaaacc gtctacatgg atacgcttga atttttaaat
     3301 aatcaatggg taaataaaaa ggagcctttc cgacaatatc cttcttttgc tgaatctttc
     3361 aatgacaatg cctatgtact tagaaatact tcatttggca acggttatta ctatgccggt
     3421 acttggaaga gcaacacgaa atcctataca gatgcgacag cttgtttgac agggcgttat
     3481 gcgactgatc ctggttacgc aggcaaatta aataatatca tcgcaactta cggattgacc
     3541 caatatgata caccagctag cggaaacgca ggcggtggag cgactacagg tggcaataat
     3601 actggaagtg caggtagtac tggcggttca agcaattctg gtaatacaaa ctcaggttca
     3661 gcaacttcga ccacttatac tgtccaatct ggtgattctg tttggggaat ctcaaataaa
     3721 ttcggtatca caatggatca attgatccaa tggaacaaca tccaaaacaa tttcatttat
     3781 ccaggacaaa aactgacaat caaaggtagc caggaaaatg gctcttcgac gaataattcc
     3841 ggtaacaata ccaactcttc aggaaatgct ggtacatcaa acggaggaca aacaactggt
     3901 gcgaaataca ctgttcaatc tggtgattct gtttggaaga tctcaaatga tcacggaatt
     3961 acgatggctc aattgattga atggaacaat atcaagaaca atttcgttta tccaggacaa
     4021 cagctaatcg tatcaaatgg cggttcttct aatgctggag caaatactgg ctcaaatact
     4081 gggaatacaa gtggctctag ttcgtccagc tcaagttctt ctaatacagc ttcaggaacg
     4141 aaatatacag ttaaagccgg agactctgtt tggagcgtct caaataaata cggaattacg
     4201 atggctcagc taatccaatg gaacaatatc caaaataact ttatctaccc aggacaacaa
     4261 ctgactgttt caaacagtgg atcaacaaac agcacgtcag gtaatacagg ttcgtcaacc
     4321 aatacaaata acagcagttc agttaatact ggcagcagtt cgtctcaagg cacttatacc
     4381 gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac gatggatcag
     4441 ttaatcgaat ggaataatat caaaaacaac ttcatttatc caggacagca attgatcgtt
     4501 aaaggtggaa cttcggcaaa tacaaataca ggttcaacta cttctgcagc gaaaccaaat
     4561 acaccgaata cgtctgctac gacaagtaca tcatccactg gtaatacgat gtataccgtg
     4621 aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc atatcacgat ggatcagtta
     4681 atcgaatgga ataatatcaa aaataatttc atctatccag gacagcaagt gattgtgaag
     4741 aaaggttcat ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac ctatactgtg
     4801 aaatctggag aatctgtttg gagtgttgct gacagtcatg gaattacaat ggctcaacta
     4861 atcgaatgga acaatattaa gaacaacttt atttatccag gtcaaacgtt gattgtagct
     4921 aaataaaata gctgttcgaa gaaataacga ttgaattcca caaaaatatt tgttactata
     4981 aggctactta aatatgacaa cacttgaaaa ggaagtagta aaaagactga agtttcagag
     5041 agcgcatggc tggtgagaat gtgcacggat gcttttgaac tcgcctttga gttgcccttg
     5101 ctgaataccc gagtaagcaa gcggcggtga cggccgttac aacgttcgag gccttttcac
     5161 taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat aaaaagagca atctttttat
     5221 aggatttttt tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa ataaaaataa
     5281 acagattctg ccggtttttt cgttatattt ataatagaaa caagtgtttt caatagaaca
     5341 ggaggacagc aaatgagtta caatcataaa gaaattgaga aaaaatggca aaaatactgg
     5401 gcaaaaacga atactttcaa cacccatgat gatcctgaaa agccaaaatt ttacgcactg
     5461 gatatgtttc cctatccatc cggtcaaggg ctacatgtag ggcatcctga aggttatacg
     5521 gcaacggata tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct gcatccaatg
     5581 gggtgggatg cattcggtct gcctgctgaa caatatgcgc tagatacagg aaacgatcca
     5641 gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc tttaggattt
     5701 agttatgact ggaatcgcga agtgaataca actgatccgg agtattataa atggacacaa
     5761 tggatcttta cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt agcggtaaac
     5821 tgggtacctg aattaggaac agttatttca aatgaagaag tgatcgatgg gaaaagtgaa
     5881 cgtggtggct atgatgtagt cagaaaacca atgcgccaat ggatgctgaa aatcactgct
     5941 tatgctgatc gcttattgga tgatctagaa ttagttgatt ggccagaaag cattaaagaa
     6001 atgcaaagaa attggatcgg tcgttcagta ggagcaaata ttgaatttaa agtggctggt
     6061 acggataaat cctataccgt atttacgaca cgaccagata ctctattcgg tgcaacttac
     6121 tcggttcttg cacctgaact agatcttgtt agagaaatta cgacccctga acaaaaagct
     6181 gctgtagaag cttatatcga agagacagct aaaaaatcag atctaaagag aacggatctt
     6241 gcaaaagaaa aaacaggagt atttactggt gcttatgcaa tcaatccagt taatggcaag
     6301 gaaatcccaa tctggatcgc cgattatgta ttatcttcat acggcacagg agccatcatg
     6361 gctgttccag cacatgatga acgtgacttt gaattcgctc aaactttcgg actagaaatt
     6421 cttccagtta tcgaaggtgg agatgttgaa aaagctgctt acacagaaga tggtacgcac
     6481 atcaactctg aatttctaga cggcatgaac aaacaagaag caatcgataa aatgaatgaa
     6541 tggctagaag aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg tgactggcta
     6601 ttctcccgtc aacgttactg gggtgaacca atcccaatca tccattggga ggacggaaca
     6661 gtcacagctg tcccagaaga agagctacca ttacggttgc ctaagacaaa aaacatcaag
     6721 ccaagcggta ctggagaatc tccattagca aatatcgagg agtgggtcaa tgtcgttgat
     6781 cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg ggcaggaagc
     6841 tcatggtatt acctgcgtta tatcgaccct cataacaaaa aagaactagc agattatgaa
     6901 aagttgaagc gttggctacc agttgacatc tacattggtg gagcagaaca tgcggtactt
     6961 catttactat atgcacgatt ctggcataaa ttcttatacg atatcggtgt tgttcctaca
     7021 aaagaaccat tccaaaaact atacaatcaa ggaatgatct taggcgaaaa caacgaaaaa
     7081 atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac ttacggtgca
     7141 gatacactgc gcatgtacga aatgttcatg gggccattag atgcttcgat tgcttggagc
     7201 gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt gatcgttgat
     7261 gagaacaata aaatgcgtga ccgtatcact acattgaatg atggcaagct tgataaagtg
     7321 tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa atcttcattt caatacagca
     7381 atctcccaat tgatggtctt tatcaatgaa gcctataaag tggacgctct gccttatgaa
     7441 tatatcgaag gatttgttca actactcgca ccgattgctc ctcacatggg tgaagaacta
     7501 tgggcaatcc ttggaaatga tggtggtatt tcttacgcac catggccaac atacgatgaa
     7561 gcagcacttg ttgaagacga agtagaagtt gttttccaag taaacggtaa agttcgtgcg
     7621 aaaagcaatg ttccccgaga cttaggaaaa gatgagctag aaaaagcagc attagccaat
     7681 gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa aagttattgc ggtaccaaac
     7741 aagttagtca atatcgttgc taattaattg gctctttgtc aataaggact gatgagttgt
     7801 gcaaaatcaa atctgggcca gaatgaactt cattctgtct cagatttttt gttatttcaa
     7861 attgattaat tgattgatca aaaaggtttt gattttcatg ttctcaaaca cgttcaatgt
     7921 ctttttcttc tgaatggtct tgtaagagat ctctgtaatc atcaaatttt tgtctattaa
     7981 tcttagtatt ttttgatgta taaactacct gaaattgaga gtacatacca aattcataaa
     8041 ttttttatta gtgggactag tgattgtatg gattttgacg cataatgaat gaagtatgag
     8101 aaaaggagag acaaatggat caagaactat ttattatcga agtattatca gctaaacaaa
     8161 aaattatcga gaaagcgaaa aaaagagcga aaagaataat gttcaagctt ttaagcttag
     8221 tatttttctc tggtttgctt tttttcggag ctgtatcgtt cctatatcgc catatgagtg
     8281 cagtagtccg ttcagctgaa gggatgcaaa aattaaataa tttgtatcac ttttcgtggg
     8341 gagttggcgg aataacaatt gttgtgctta ttgtttttct tctacttatt tggctccgtt
     8401 accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa cagtttttac
     8461 ataataaacc aacttctcaa gatgatgact atctttattt tgagaagact tcgacgagtc
     8521 aagcttttcg gatacaaaaa agtgattgcc agttattgct gagatcactt gatgatttac
     8581 ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa cacattccat atttttttac
     8641 tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc tggtggatac ctgacaacta
     8701 agatgagtac attagtcact ggttttttcc tcattctttt agctggaaca atctattatg
     8761 ggagatacac tcagccaaaa atgtcttctc atataaatgc tgcttctcac atgacaatga
     8821 actcaactaa cgatacgcat atacaaaaga caaatgaagg gttgttagct gatcctaagc
     8881 aaaaggtaaa tttgacaaca caaaagataa atgatctcaa acttgataca gagacgcact
     8941 ctctctatat gacaacagat agtggtgcta attgggtatt tgtgccgatt gattcgcaat
     9001 ggttgcgttt tggcgaatac accctgacaa cggggctgat tcctgaaggt tattggatgg
     9061 acaacacata tgatatttcc gccgatttct catggtttat ctattcttct ggggatgatg
     9121 tacgtctgct gacttcaaga gataatggta aaacatggca aacgtctatt atcacccaat
     9181 cagttcagcc gatacgttat cgaaaagcgg tattttatgg caacgatggt ctatttatct
     9241 gtacatcagc cagtgggatg tctgcagaaa ctttgtacat ttacactact atggatggcg
     9301 gaatcacttg gataaaaagt ggaagtacaa tgatcaatca gccagtacaa aatgtcagct
     9361 tcgttacaac ttctttaggt tttgtttcga cacgtgaaag tttgttttat acaaacaacg
     9421 gaggacgctc attcaaagaa tcagttctgt cgattccgga aaactatcag acagggggga
     9481 tagatctttt tcaatcacca aatgaagtta ctcaaataag cgcgaatacg ttagagacaa
     9541 aattttattt gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc tgcctcttcc
     9601 gttcaactga taacggggaa acatggcaat tcgttcagca actatcgcaa gtagaacaag
     9661 gagattaaag aagagagatt gtgagcctga cgttcagcac cgagtcacaa gaaacaaatt
     9721 caaaaaacag cttctcatat ttgttgaata gtgtaactcg tgattcggag ttgacaagag
     9781 caacaagaag cgattttgaa gtatagcttc tcgtatttta caagatcgtg tcttattgcc
     9841 gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa gtcttttgtc
     9901 actacttacc gaacgaaact gaaggagcta tccaaaaagt atcattagta ttattctttg
     9961 tgtttctagt ggcaccacta gtacatttaa aaaagctatc gatcatggac taatcataca
    10021 tgatcgatag ctttttagat aatgtttgtt acgctatctg aaattgacta ttataaagac
    10081 tggcataaag cgttggtgat tggagtagtt cagaatgtgt tcctttttcg acgatatcgc
    10141 cattcttcat tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg tgtgcaatca
    10201 caaaacttgt cctatctttt gtcacagctt ccattgcttg ctgaatcaat gcttctgttc
    10261 gtgtatctac gctagaagtt gcttcatcta aaataacgac aggaggattc gctagaatga
    10321 ctcgagcaat tgtcaataat tgttgctgtc cttgagataa cgtgccgttt tcgctagaaa
    10381 tgatcgtatc atatccgtct ggcagtgttc gaataaagtg atcacattgt gcgatttcag
    10441 ccgcttcgat aatttgttct cttgtggcat ccattttccc gtaggcgata ttatcagcta
    10501 ccgttccttc aaacagccag gtattttgta aaaccatccc aaaagaacga cgtagttctc
    10561 cacgtgaaag cttggtaata tcaagaccgt cgaattcgat ctttccttta ttcagttcat
    10621 aaaaacgcat cagcagattg actaaagtcg tttttcctgc accagttgga ccaacgatcg
    10681 ctaccgtttg ttttggcttt actgaaaaat cgacttgatt catcaatatt ttatctgatg
    10741 tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt cggttgttct aaaagtcttg
    10801 catctggaag gtctggaatt tcatctgttt catctaatat ctcaaatatc ctatcgattg
    10861 ctgccattgc ggattggatc gagttgatca cataagaagc ggtagaaatt ggttcagaaa
    10921 tctgattgat gtactgaaga taagcttgta agaagccgat cgtcacacca cctgatagaa
    10981 caagcatagc accgaatacc gcacttatga tgaaggcgag ttgattaatg aaacgaatgg
    11041 ccggatagat agcacagttc ataaattgag cacgtttaaa agcgttgtat tgctttttat
    11101 ttacttcgtg tatagcagct tctgcatgag cttgctgatt gaatgttttg accactagat
    11161 taccagctaa gatttcttcc attcgattat ttaaaacacc cagttcatac tgattctgtt
    11221 cagaaatctg tttgtttttg ttcgcaatct tagttgtaac aattgaactg ccaacgatca
    11281 gcaataagac gagcaaagta agcttgcttt cgatataaaa cagcatcaga ccagcagcag
    11341 ctattgttac gacagaagta aaaaattgat tgatacctgt caataacact tgagaaagtt
    11401 gattgagacc ggtagttgtc cgactaatga tgtcacctac ttgatgattg tcaaaaaacg
    11461 acatcggtaa tgttttgaat ttttttgtga tttcttttcg caccctcaat gtgatctttt
    11521 cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt gatgctgctt aagactgaac
    11581 agccgatcag caataagaca gggaacaaca atgcttgttt gatatcactg aaagaaagct
    11641 gtccaatacc tgtttttctt atttctgata agagatcatc gatcccgatt cccatgatca
    11701 agggcatggc tacgactaat aggttcccga ataaactgca tagaaacaat ccgtaaaaat
    11761 atttatgttc aggtttgatc attttccaaa aacgtatcaa agcacgcgtg ttcaacgttt
    11821 ttttcttttc catcgtatcc cttctttcta tattccttgt gattttgcaa actcctgata
    11881 agtttggcaa ttagacagta gttcttcatg agtaccttgt ccaacgattt ttccttcatc
    11941 taaaacaatg atgttatctg cttgttggat tgtactgagg cgttgagcaa caatcacgag
    12001 agttttctcc gacatgtgtt tatgaagggc tgctcttaat gctgcatctg ttttatagtc
    12061 taaagcggag aaactatcat cgaatacata gatctctgct ggtttgatca acgctcgagc
    12121 aatacatatc cgctgtttct ggccccctga ataattagaa ccgccttgag caacaaagct
    12181 gtccaatccg tcaggaaggg tgtccactag ttcttctaat tgagcgatct tgatcgcttt
    12241 agacaactct tctgttgagg catattcatt tcccatctgc aagttgctta aaattgtccc
    12301 gctgaataag aaagccttct gagggacata actgatgtgg gagcggatct gttcttgagt
    12361 catttgagta agaggaatac caccaagggt tacttttcct atcgtagggt cactaagtct
    12421 caagagaagt ttcgccaaac tactttttcc agatccggtc ccgccaacga tcgctgttgt
    12481 ttttccttta ggtattgtga aatcaatatc ttctagaact ggatcagaga tttcagtata
    12541 tttaaatgtg acatgttcaa aagcagctgc atttgtctga tcaaaggttg gttcagctac
    12601 tgttttgatc gtctcttcct caatttcgat ctctgcttgc agtatttctt ctatccgtcg
    12661 taatgaggca aacgatttag ggataatgac caataccata gctgcgatca tcaagtaagc
    12721 aagtgtcagc atagagtatt cgactacagc agtcacgaca ccgatttgta atgctccttc
    12781 tgctacaaga tttccgccaa accacaaaat agaagcataa acgattccca tgagcaaaaa
    12841 agcaattgga gtgacccaag caaatattcg gttcactttg atcatttgat tagcatagtc
    12901 agcaaagctc ttattcgttc gcttttcttc atatgattga ttatcaaagg cacggatcat
    12961 attaatccca gtaaagaatt ctctcaatgt gatggtgatc cgatccattt ttggctggat
    13021 actcaaagat aaaggtgccc ctcgtttcat cagtcgataa acaatccata cataaaaaac
    13081 aacagagatc aatgtaaccc atgttagtaa tagagaataa cgaaacatca tgagaatggt
    13141 gaaaacgcaa ataaccggtg ctggtaagat catttggcaa aataaaacaa tcatttgctg
    13201 gacattatcg acatcattcg tcattcttgt caaaagagat ccagaactaa aacgactgat
    13261 atccttcatt gacagctgtt ggaatttacg gaaatatttc tcccgcgttt ggaatccaaa
    13321 cctagcggaa gccaatgctg aaaagtaact agaggcaact gcagcgattg ttcctagaaa
    13381 cgctacaagc agcatttcaa ctccaatttt ttttataact gaggcttcgc cacttgctat
    13441 acctgtgtcg atcagctttg ctactaattt tggtacacct aatgtaccgg cgatttctaa
    13501 acagatcatc acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga atgtttttaa
    13561 gatagacata ttattcctcc ttttttcctg atacttttcg attctatacc ttcaagtaac
    13621 ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat gaaaaaaatg
    13681 tatacgattg gccaagtatc ggaactattt gatttgccaa aatcaacgat ccgctattgg
    13741 gatgaacaag gattaatccg ttcaagtaga caagaagaga atgattatcg attgtttgat
    13801 atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga tataccgatc
    13861 aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac acttgatgaa
    13921 acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa agagataagg
    13981 gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt tcctgtagaa
    14041 gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga aatcaaaaaa
    14101 tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag ttcacagacg
    14161 atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga cgtgatttgg
    14221 gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat gaaccatgcg
    14281 gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg gaaaacagga
    14341 gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag aactgaatat
    14401 tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa ctcgaaaatg
    14461 atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta atgtggggga
    14521 ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct ttaaaaatgg
    14581 aatggttgca attcttagcg atatatacga tgtaattgtc ctcttaaaaa agtatggaaa
    14641 aataattgta ttgggtgtta tgtataattc atggtgaact gagactaatg agaagaaaaa
    14701 ttcatattgt tatgagtgag attgtatcag atgagaatga taaattaaat aggtgcataa
    14761 aagcattaca cttaaaatac attgttgctt acattattac aatatgttaa taaggcaaaa
    14821 ataaatagat ggattgaata aacatatttt ttaatcaaat agtccctact gtgactttcc
    14881 tcagctatcg gtgatactac cgatagcatt tttttgctta taaaataatt ataagtattt
    14941 agagattttt cttctaacat tcttcaaaca agaaaaatca caaaggttat tgttattttt
    15001 atatattctt tttaagaatg attgtttttt tatagaaatt ttaaaaaata aaacacgtta
    15061 ttgatggata gttagtttgt tatttggtaa tattatagaa gttgactggt agtgatttat
    15121 ttttcgtcgt tttataaaaa tgttattttt tggaaaaatt cacaaatatg taacgaatgt
    15181 ttttctggaa tttgctgtgt taaaaagcag tatgactaat ataaatacta ttgtcattta
    15241 tgcaattttt tatggatagt aattttagag gggaatcaat gaaggaagaa tagaagggaa
    15301 aaatagttgc aaatacgcca gttagcaaaa atagcaatac atcttcaagc tttaaaattc
    15361 gagagaatca tcttggtagt ggaatcaact acaacaaaag gattaaacca attaatagat
    15421 agaattacca atggaaaaat agaaaaggta gttattctat acaaaagttg tttaattcgc
    15481 ttcggttatg aacttagtga aaccctttgc aatatatggt acagaatata gaaattattg
    15541 acattacaga aaatgatgaa gatttaatac agattgtcac tatttgtaga taccttttac
    15601 aaggaaaccg agccaataaa gctaagaaga tgattaagga gttgatggaa gacgatacaa
    15661 gttaagaaag tgaaactacg cccaaataaa gagcaaaaag agtaattgga cgtggaaaat
    15721 gcaagaaatg aaccatcgat tcggtggaca gtttattagt aatgacggtt tgagaacatc
    15781 catgacgaga atgaaggaac atcctaaatg cgtttagttg aaccaagtgc caaataatat
    15841 ggccaaataa gcgatcagtt atcgatgaat cccaagtatt ctaaactata tattcatttt
    15901 gatgctaagc attgatatat ttttttggga ttgatgaagg ttaaccaata cagggactca
    15961 ctgatgaaat tctagagtct gaccttggta tcaacgattt agtgattgtt tctaatggaa
    16021 aagtataaaa gtatataagt acattaacaa aataactatt agggttacgc tccagcttgt
    16081 gcaaggcact ggcaggagga atcgtgaaat cataattatg gttaagccac acggacaact
    16141 aaaagtcata caaagctatc gaatcaatca acagaagaca atcggcaatc attagctttg
    16201 tttacagctt acattgcaac gcttgagttg aaccgaaaat acgagggagg taacgcagcc
    16261 attttgagtt ggggaatctt tttagaaaat tatcgggtac cttgttgttt agtcaaaatt
    16321 ataaggggga gtaatagtga ggaacaaatt tcataaactg ttaagtatgt ttctagttat
    16381 gcttattgtg atcaatcaat ttgggaacat aacggttcta gctgagtctt tagcagaaca
    16441 gccagagcgt tcgctgacga tcgaagttga tcaagaggag gctgctttgg gtgagcagtt
    16501 ccagttgcga gtcagaggaa caccagacct gctaaatgca ttggtcattg ataagagtga
    16561 ggatttgatt gaaggggaag tgaagcaact caatgattac gaacgtctga ttacttattc
    16621 ttcccagcat tcaggtaaat acgaattgac cgcaagaagc ggtgatattc aatcaaatac
    16681 ggtctcaata gaaatcagtg taccagaaga aagcacaagt gagtctttta atgtaccaga
    16741 agaaagcaca agtgaatctt ttaatatacc agaagaaagc ttaagtgaat cttttaatga
    16801 agccagatca tctgagccat ctataacgca ggaatctcag attgaaagac aagaggagct
    16861 cttgaattat gacatcggca tctctgctga gcgccccact ttgccgagca ggcatacaca
    16921 aacgttaaag ggatacattc aaggaggcgc tggcgatatt cttgaagaat atggggagat
    16981 ggtcgtacga gttcctagag catcgattgg cttaattgag ttcattagag tggaggtcgc
    17041 tgatgattca cctttaaagc cactggcatt ttaccaagaa ggcacagacg ttgtttttcc
    17101 atttaccttt gattatgaag gattaaatga agccgctcag tcgtatacat tcagtatcac
    17161 gtttgaagtt gctttctttc aatcaggcgg tactgagcca aataatgact ttcaagtgag
    17221 tcttacttat gctgagcgag cagcaaacac ggaattttca attacgccta ttccttcatc
    17281 aagacccatt ttttcgaaat tatactatgg ctctagagaa atcgacagcc aaggaaatct
    17341 tgggaatctg ctgattgacg ggcaagacgt gaccaacagt aataaattta ttttgcaggt
    17401 aaattatgaa ggatcacgaa aagacggtgt agtggttacc gatgtactac cgcaaggaac
    17461 aactttggca ccgaatccga ctacatggag ttccactgga aatagagaac ctatttcagg
    17521 tggggttcgg atcgcagaag taacgaatcc aggtgaagca aacgttagaa ctcattatgt
    17581 ttccgaggaa tttgtggaca aagtatcttt taataaggaa acgaatacac ttgttgtgga
    17641 ctttggtaat ctagcagagg gtcaatcgta ttttattgaa tatgctcttg aagtgaccga
    17701 tgtcgacttg ttcaatgaag gaagacaaga ggatgggttt tctcggaatc gagcgagtat
    17761 gacctacaat aatcaggtaa tgagccgaga ctaccgcttg agaatccctg aaagtaatct
    17821 tccggcaatc tctttaagaa aaagtgtgga caaaaatgtt atcaatctca atgaaaatga
    17881 actaacgtat actctgcatg tctcgatgtt ttctggaaca agaaatacgc ttgttattga
    17941 agatgttttg gaagaaggca tggaatttgt gtcatttcta tcagatattc cctctttcat
    18001 tgatgccgaa gtcaataatg gaatagttcg tttcagtgcg aataatacga ttggtggtcc
    18061 gacaagtttt accctctctt ttaaggtcaa tgtagaggcg tatgcggtcg gcgatcaaat
    18121 tttaaacttt gcaacgcttg tgaatagtga caatcgattg aatacgagta ctgtaagaac
    18181 aaggaaaatc gatgggcgta ttaaagtaat taaaactgat gagaatggcc aaaggctggc
    18241 aggagcaaaa tttgaaattt tatcaagcga ttcacaagtg atacaagaag gtataacggc
    18301 agaaaacgga gaatttttaa gtgaaccgct tcctgtaggt agctatcaag tccgagaaac
    18361 agaagcaccg gaagggtatc ttttagatgc gacggtacat gatgttttta taaatgaata
    18421 ctccatagaa gaagtgacgt taaccattga aaacatcctt gatacgggtg gtgttgaact
    18481 aattaaacat gctgaaggca gtggtgaagt ccttcaagga gctgtttttg agttacagaa
    18541 ccgagaagga gaaacgctac aa
//
LOCUS       DACCSK010000052        18504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCSK010000052 DACCSK010000000
VERSION     DACCSK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.053"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IVP39_001560"
     CDS             99..746
                     /locus_tag="IVP39_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7586635.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IVP39_001561"
     CDS             1139..3784
                     /locus_tag="IVP39_001561"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7586636.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IVP39_001562"
     CDS             4094..5407
                     /locus_tag="IVP39_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7586637.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IVP39_001563"
     CDS             5397..6050
                     /locus_tag="IVP39_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7586638.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IVP39_001564"
     CDS             6105..6797
                     /locus_tag="IVP39_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7586639.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IVP39_001565"
     CDS             6947..7147
                     /locus_tag="IVP39_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7586640.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="IVP39_001566"
     CDS             complement(7401..9080)
                     /locus_tag="IVP39_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7586641.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="IVP39_001567"
     CDS             complement(9082..9294)
                     /locus_tag="IVP39_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7586642.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="IVP39_001568"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="IVP39_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7586643.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="IVP39_001569"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="IVP39_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7586644.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="IVP39_001570"
     CDS             13269..13466
                     /locus_tag="IVP39_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586645.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="IVP39_001571"
     CDS             13619..14104
                     /locus_tag="IVP39_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7586646.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IVP39_001572"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IVP39_001572"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7586647.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="IVP39_001573"
     CDS             14981..15508
                     /locus_tag="IVP39_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7586648.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="IVP39_001574"
     CDS             15599..16336
                     /locus_tag="IVP39_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7586649.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="IVP39_001575"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="IVP39_001575"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7586650.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="IVP39_001576"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="IVP39_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7586651.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5983 a         3201 c         4023 g         5297 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt atcttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acgaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DACCSK010000053        17184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCSK010000053 DACCSK010000000
VERSION     DACCSK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.054"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IVP39_001577"
     CDS             complement(543..2048)
                     /locus_tag="IVP39_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7586652.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLFENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVP39_001578"
     CDS             complement(2045..2731)
                     /locus_tag="IVP39_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586653.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVP39_001579"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVP39_001579"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7586654.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVP39_001580"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVP39_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7586655.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IVP39_001581"
     CDS             complement(4752..5321)
                     /locus_tag="IVP39_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7586656.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IVP39_001582"
     CDS             5507..6409
                     /locus_tag="IVP39_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7586657.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IVP39_001583"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IVP39_001583"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7586658.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IVP39_001584"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IVP39_001584"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7586659.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IVP39_001585"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IVP39_001585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7586660.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IVP39_001586"
     CDS             12977..13162
                     /locus_tag="IVP39_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7586661.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IVP39_001587"
     CDS             complement(13224..14420)
                     /locus_tag="IVP39_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7586662.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IVP39_001588"
     CDS             complement(14590..16026)
                     /locus_tag="IVP39_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7586663.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IVP39_001589"
     CDS             16319..17128
                     /locus_tag="IVP39_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7586664.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5043 a         3594 c         2983 g         5564 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa acagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct tttt
//
LOCUS       DACCSK010000054        17165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCSK010000054 DACCSK010000000
VERSION     DACCSK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.055"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(180..2087)
                     /locus_tag="IVP39_001590"
     CDS             complement(180..2087)
                     /locus_tag="IVP39_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7586665.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2217..3092
                     /locus_tag="IVP39_001591"
     CDS             2217..3092
                     /locus_tag="IVP39_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7586666.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3172..4146
                     /locus_tag="IVP39_001592"
     CDS             3172..4146
                     /locus_tag="IVP39_001592"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7586667.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4335..5471
                     /locus_tag="IVP39_001593"
     CDS             4335..5471
                     /locus_tag="IVP39_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7586668.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5608..5952
                     /locus_tag="IVP39_001594"
     CDS             5608..5952
                     /locus_tag="IVP39_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7586669.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6223..7509
                     /gene="efmA"
                     /locus_tag="IVP39_001595"
     CDS             6223..7509
                     /gene="efmA"
                     /locus_tag="IVP39_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7586670.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7647..8246)
                     /locus_tag="IVP39_001596"
     CDS             complement(7647..8246)
                     /locus_tag="IVP39_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7586671.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8650..9771
                     /gene="mnmA"
                     /locus_tag="IVP39_001597"
     CDS             8650..9771
                     /gene="mnmA"
                     /locus_tag="IVP39_001597"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7586672.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            10017..11438
                     /gene="arcD"
                     /locus_tag="IVP39_001598"
     CDS             10017..11438
                     /gene="arcD"
                     /locus_tag="IVP39_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7586673.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11452..12570
                     /locus_tag="IVP39_001599"
     CDS             11452..12570
                     /locus_tag="IVP39_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7586674.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12630..13922)
                     /locus_tag="IVP39_001600"
     CDS             complement(12630..13922)
                     /locus_tag="IVP39_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7586675.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14072..14632
                     /locus_tag="IVP39_001601"
     CDS             14072..14632
                     /locus_tag="IVP39_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7586676.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14761..15138
                     /locus_tag="IVP39_001602"
     CDS             14761..15138
                     /locus_tag="IVP39_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7586677.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15071..17143
                     /locus_tag="IVP39_001603"
     CDS             15071..17143
                     /locus_tag="IVP39_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7586678.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5480 a         3112 c         3652 g         4921 t
ORIGIN      
        1 ccattttcac aaaatttttc ttactacttg aaactaagca gctttttcac aacctctaat
       61 acatggtatt ccagaagtaa cttcttcgaa aaataagccg aaatgtcaca aaaatttgag
      121 aagcgatttt cccaaattcc ttcttatttc ttgaagctga ccacttctgg cacaacctct
      181 taccagattc gcactctttt ttctggcgct agatacatag cgtcttgttc cgtgatacca
      241 aatgtttcat agaattcgtc aaggtttttt ggttgcacgt ttgctcggag tttagcaggg
      301 gcatggacat cgatttgcag taaaagttgt tggtattctt tttttgcttt tgtacgccaa
      361 atcgttgccc aattgacaaa gaattcctcg atagaaagat ctgattccgt ttttgctgct
      421 tccaatgcac agcttagacc gcctgcatca gcaatatttt ccgaaacggt taattttccg
      481 tttacttttc cgtcagcaaa aggcagacca tcgaattctt cgatcattgc ttgtgctttg
      541 ttttgaaatt gtgtaagatc ttcttctgtc caccagttgt tcaagttgcc atattcgtca
      601 aataaagcgc cgttattatc aaatgcatgg gaaatctcat gagcgataac agctccgatt
      661 ccaccataat tagcagagct tgattgttct agactgtaaa atggtgcttg aaggatcgct
      721 gccggaaaga cgatcacatt ccgaaatgga tgataataag cattgaccgt gtcagcactc
      781 atttcccatt ctgaacgatc cactggtttg ttccatttgc tgaaacgatc ttttaatgtg
      841 agtttgctga aaacaagagt atttgaaagc aaagtaccac cctgatctgc aggtactgtt
      901 ttgaactggt cgaataatgc tgggatctta tcggggtagc ctacttggat acctaagtta
      961 tttaatttta tgacggcttt ttcttttgtt gcatccccaa gccagtcatt ttcttctaga
     1021 cgttttttat aaacggcgat catttttttc accatttgtt ctacgtcttt tttagcagct
     1081 tcgccaaaat atttttttcc gtagtaatcg ccaactactt gatcgaattg tccagaggca
     1141 aggtaatacg ctgctttttt ctgaggcatt gcttcgtctg ttccagacaa tacgcgagaa
     1201 tagattccgc tgacttggcg gaattcttct gatagataac cactaagaga acgtactgtt
     1261 ttaatgatca tccagctttt gaataacgga aaatgagctt ctgtcaggat gtcattcaaa
     1321 tgatcgaagt acactggatc agttacgatc actttatctg gagttgtccc aatcaaaccc
     1381 gtgacaagtt ttttcaagtc cagtttatct gtgtatttaa taaatgtatc aaaagattgc
     1441 ggattataca ttttgctgta atctgcgttt tcttctgcac tttttacatg aggagcaatc
     1501 aatttatcaa attgcactgc ttgttccgtg attgcttctg cctgactttt ttctttgccg
     1561 gcaagctcta ataactgaac agccatatca aagaaaacgc ctattagttg tgcaccgttg
     1621 ggatggtctg atgcataata cgtcttatct ggaaggaata aagaaggcgg ataagcaaag
     1681 aaggcgttcg tttgcgcatt cttcatatct gcatctacat ccagattaaa tggcaatggt
     1741 aatgtatcca acagccattc tggaaattgc ttattcaatt cgtcgaaaga ctgcaatgat
     1801 tcaacttttt cgattatagg ttgaagcggt tgagcatgca actcatttct tcggtcataa
     1861 tcgtttgcca gttgataata tgcaaggaaa tgtttcattt tttctgaaga cactttttct
     1921 ggatgggcaa gcagatcgtc tgtttctccc atcaaaagtt ggtcgatccc gtcaactaaa
     1981 tcttggaaac cgcctgtagc tggtttatct gcagggattt ttgctgtttt ttgccattct
     2041 tcattgactg cttcatagaa atcttgtttc aatcgttctt tgttcattca tcgatcgctc
     2101 cttttttgtt tcgtgtgccc attatcttac aaaatgaagg aattaacaaa tagaacataa
     2161 aaaattgaaa attcgaacaa tcaaactgat ttgttttttt caatccttca cttataatga
     2221 aaaaaaggag gcgatcccaa ctgaatcaag tagtggataa acctttatat ttcaaagtag
     2281 ataaaaaaat taaaaaatta gccagcactc agcagctgca gtcaagaaaa agtaaattgc
     2341 tttttttagc tctggttttt gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc
     2401 cggtagaata ttctccagca aaatatactt atcaagaggg tctttcttgt aaaaaatgga
     2461 agctgatcaa tgaagagcca atcgagttat ctagatggat caacagaaaa gaagatattc
     2521 ccgttttgat cgaagaaaaa aggtcaggaa aggaactcgc aaattgctgg gtgggattgc
     2581 cagaagaacg ttttttgagg tataagaaat gggctacccc ctcaggctat ctttgtggta
     2641 cttacgcggc agcagttctc cttgcctatt atcaagacta tcgtaagggg tggatgcttc
     2701 caaatgagat tcgcaagaaa aatacggctg attcgttggt tttgacgaag gcattgcgta
     2761 gccagatcca accactagga cttcctacga ttccttttca ggttagtaca gggatttcta
     2821 gttttttaaa gaagaatggc aatcatgagc gtgcacgagc aactcttcta ggttcatggc
     2881 agcgagctac aaaacgtata cgtgaaggca aacctgtgat gattggtatt ttgaaagtac
     2941 ttggcagtac atatggcaat cattgggtga cagcgtacgc ttattttgaa actgaaacag
     3001 gagaacgtta ttacaaagta cacgacaact ggggcgatta tcataaagtc attcctgcca
     3061 gttggagtaa cggaacagtt tcacttcctt aagaatttgc tgaaattaac agaaaatctt
     3121 ggaaaaatat gttaaactat cgaagaatag agaagttttg gggaggacta catgagtaat
     3181 aagtatcagg atgacacgtt gaagattttt agtttgaatg gcaaccgtcc attagctgaa
     3241 aaaatcgcga aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca atttagtgat
     3301 ggcgagattt ccatcaatat cgaagaaagt attcgagggg atcatgtgta tatcgttcaa
     3361 tcaacgaatg cacctgtcaa tgattactat atggaactac tgatcatgat cgatgcaatg
     3421 aagcgagcaa gtgcgaaaac gatcaatgtc gtattgcctt actatgggta tgctaggcaa
     3481 gaccgcacgg cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa
     3541 gaagcagggg caactcgagt actgacttta gatctgcaca cggttcaagt acaaggattc
     3601 tttgatattc ctgtagataa tttatttacg atgccgttgt ttgctcatta ttaccgtcaa
     3661 ttaggtctga cgggagatga catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt
     3721 gcacgtagtc tttcagaata tttgaacagc acattagcca tcgtagatca tgcagatgat
     3781 gcttcaactg agggtggtta tgtgatcggt gatgttcaag gaaagacatg tatcatggta
     3841 gatgatatct tgaacacagg agcaacgctt gcacgagcag caaatgtatt aaaagaaaac
     3901 ggcgcaaaag aagtatatgc ctgtgcgtca catggattat tgtccgatca tgcgaaaagt
     3961 attttagaca atgcaccaat caaagatatt tgtatcactg attctgtttt cacagaagaa
     4021 aatcgtcatc cagaaaactt aacaatcgtt acatgttcag aattaatggg tgaagcggtc
     4081 aaacgcatcc atgaaaatac accgatgagt ccgttattcc gtctggaaga aaaagaattc
     4141 gaataaacaa aaaaaagagg gaaagaagtt gtgacagaag tgagcagctt caaggagctg
     4201 cgacaagact tttgtcacag ctcctttttt ctatgcccat gaaacgtcct ctagacaaaa
     4261 atgagctctt ctttggtttg ttcgctattt tatgctagaa tgtgcctagc aataatttgg
     4321 aagggatgaa tgaagtgaaa actatttatc tggatcatgc ggcaactacc cccatgcatc
     4381 cacaagtaat tgaagcgatg acggcaatca tgcaagacac gtttggaaat ccttcaagca
     4441 tacatggatt tggtcgccac gcgcatgaaa aattggaaac agcaagacaa atcattgcag
     4501 acagtctgca agcaaaacct cacgagatca tctttaacag tgggggaaca gaaggggata
     4561 atacagctat cttagaaaca gcgttctcgc gtcaaaaaga aggcagacat ctaatcacta
     4621 cagctatcga acatcctgct gttcttaata ccatgaaata tttagagaca aaggggtttg
     4681 acgttacata tctgcctgta gatgaaaaag ggaatctgtc gatcgaagag gtcaaaaaag
     4741 cgttacgaga agatacgatc ttggtctctg tgatgtacgg gaacaatgaa atcggaaatt
     4801 tgatgccaat caaagaaatc ggtgaacttt tagcggatca tccagcttat ttccatacag
     4861 atgcggtaca agcgtacgga aaccaaacga tccatccaga agaattagga atcgatttat
     4921 taagtatctc tgctcataaa atcaatgggc caaaaggcgt cggtttctta tataaacgag
     4981 acggagtaaa tatccctgca ttgcttttag gcggggagca ggaagaaaag cggcgtgcag
     5041 gtacagaaaa tttagcaggg atctggggta tggcaaaagc agttgaactg ctgtcacctg
     5101 aagaacgtga agcacgtaac aacaaatata aaggattcca gcaattgatt cttgatacat
     5161 tagaagcaaa cgaaatccct tatcaagtaa atggtgattt aacgaataaa ctccctcatg
     5221 tgttgaatct tcgcttaatt ggcgaagcaa atgatttgct actgatgaag cttgatctta
     5281 atggggtcgc tatttctact ggttcagctt gtacagcagg aaatatcgag ccttcacacg
     5341 tattagaagc aatgtatgga gaagatacac cgatactaaa agaatccatc cgtatcagct
     5401 ttggtttggg aaataccgaa gacgacatcc agtatttcct tgatcaatta gttcgtgctg
     5461 tcaaaaaata actagtgaag tcattcttat atgaacagat gtctgagaga acgccttcat
     5521 tttttaggga acttaactga aaaacctagt aaaaatcagg ataaactttt ataataaaga
     5581 gaaactaatt ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac ggtactgggc
     5641 gcaccaatta gctatcgttt gcatcctgat gcaaaaagat atacattgcg cgataatgga
     5701 tttacagaaa caaacggagg caattatcaa ttgatccgac cattagatgc cacgccgcaa
     5761 agcaaagaag gattcaaatt aaaaatcact gtatcaaaag atattcaaac attgaaaatg
     5821 tcgatcacta cagcaaatgg attgaaagca gtcaatattt tcaaagatcc aaaacagaaa
     5881 atgagccaag ataaatttta ttttctgatg gatgggatga ttagccgtgg gttattcgaa
     5941 aaagtcgaat gatctgtgga tataaaaaga caactagttt tgaaacggcc tattgttaga
     6001 tgactaactt tgtaaggacg acgcaaatta gttgtctttt ttatttttaa atttaacaca
     6061 tgttgttatt tttaatattt tgctgattta ttgtttctaa gtaaagaaat ttaaattgaa
     6121 acgattgtta tttagggaaa aaggtatttt ttgaaaaaag aatcccgtat aatgattgtt
     6181 gtgcaaacgc tatacaaaat gaaaaagaaa agagggatgt ttatggaaaa tgaacagtcg
     6241 gtcgttttaa cgaattggaa gagaaattat ttgttctttt tatcgggaca gtttttgtca
     6301 ggaatcacta gtatggtcgt ccaatatgcg atcatctggt atttgacaag agaaaccggt
     6361 tcggcgacaa ttttgagttt tgctacttta ctaggaatga ttcccatggt tttactaagc
     6421 ccatttgtag gaccgctagt ggatcgctgg gataaaaaag ctcttttgat tgtcacggat
     6481 attattgtag cgattttcgc gcttatttta gcagtagtcg gaacgatttc agaatccttc
     6541 ccgatttggc tagtttttgt gtctttgttt atgcgttcgg tcgcacaaac attccaaatg
     6601 ccaacgatcc agtcgattat gccgacaatc gttccatctt ctcacataac aaggacgaat
     6661 ggacagttag gaatggtcca atcagcgaat ttcatcattg cgccagctct aggtgcggct
     6721 ttgttttcag tcgttcctgt caattacctt attttattag atgtattggg ggcagttttt
     6781 ggtgtcggct tattgatttt tgtgaagatc ccaaaagttt ctcctgaaat attggaagtt
     6841 ccgcttacta tttttaaaga tgcaaaattt gggctgcagc aattgatgga taataaaggg
     6901 ttatggtaca taacgatcaa tggtgcattt gtgatgctgt tgttcatgcc agcaatcagt
     6961 ctgtatccat tgatgacgct agattacttt ggtggttccg ttggacaggc gggagcagtg
     7021 gaagtagtct acgcagtcgg aatgctctta ggcggtgcac tgatcagttt tataggaaca
     7081 tggaaagacc gaatgaaacc tattattata gcctacatca ttatgggcct gacgatcggt
     7141 gcaagcggat tggttccaaa cgatagtcaa gggttcttgt actttcttat cttgaatgca
     7201 ggagcaggtt gtgcaacgcc atatttcaat acactgctga tggcaatgat ccagcagagt
     7261 tatgaatcga atgttttagg tcgtgtctta gggaacttca actctttgat gaacctggca
     7321 ggtccaattg gattattatt tgcaggacca ttagctgatc gtttaggggt agaaaaaatg
     7381 ttcttgtttt ccggaatcgg gattttatta tgcggaatcg ttttattttt aacgtcagct
     7441 gcccgaaatt atgataaaga attacagaaa aaactggtaa aagaacacca tgagcaaaaa
     7501 gacgaatagt atggaatgga gttgtgacag aagtcttgtt acaactccat ttctgatcct
     7561 aatgttcctg tggcaataag cctactcaaa tgaaaaatat gagtaattat ctttatttgt
     7621 ttaatacttg atatagggca gttttttcac gacctatcgt acttcctctt tgcttcatag
     7681 atttctttga tttgttgctc agataacgga gtgccaaatg gtagtttttc ctttaagagt
     7741 tctctttccg gtgcgtctac gcctacattg acatcgttct cgataggtac gctgtaatga
     7801 tggatatgtc catgaagatt gcggatagtg tcgttaggcc cgagcaacat cggataatga
     7861 gtcaagtaat attgttgatg gtcgaattta acgatggcac ctacatcata aaaataaaat
     7921 ttgggacttc cgtcagtaag cctaggatca tttttttcta aataacggaa aaaagcgcga
     7981 ctgtcatggt tccctttgat caagtggatt gttccttgca gttgagtgac cagttccaaa
     8041 atttctgcat ttgctttttc atattgcgga tgcatagcaa ggtcgcctag atgataaaca
     8101 tggtcggttt cttttattac tgcattccag ctagtgatca actgttggtt catttcatcc
     8161 acatttgcaa agagccgcgg agcaaagtcg ttttttccta ataagcgttc atggaaaaag
     8221 tgcatatcac tgataaagta attcatgtat cttttgcctc ccttttttct tagagtaaac
     8281 aaaatcactt atctgagcaa ctcataacat ctaaaaagaa aaggttgtaa atgagacgtg
     8341 cagctttaag cgataagaag caattttgaa aaatagtttt tcacaattta taaaattatt
     8401 ccttattgcc gaaaggctgg ccctttttat gagtatgcca attttttata tcgaatgctc
     8461 aaaagatgag tgattttttg gttgactatc atgtttgtgt actgaatagg tccagtagaa
     8521 cagtattttt acgacttgat aattaaggga tgaaattttc agaaatatga cgtataatgt
     8581 aaaatactga aaagttgcga ccttcaactc gtagatgttt cagaatctaa ttgaaatgat
     8641 ggtgatatca tgacagacaa cagcaaaaca cgcgttgtcg tagggatgag cggcggtgtc
     8701 gactcatctg taacagcact tttgttaaag gaacaaggat acgacgtgat cggaatattc
     8761 atgaaaaatt gggatgacac ggacgaaaac ggtgtatgca cggccacgga agattataag
     8821 gatgtagcga aagtagctgc tcaaatcggg attccttatt actctgtgaa ttttgaaaaa
     8881 gaatattggg atcgtgtatt tgaatatttc ctagccgaat accgcgcagg acgtacacct
     8941 aacccagacg tgatgtgcaa caaagaaatc aaattcaaag cattcttgga ttatgcaatg
     9001 gatcttggcg ccgattatgt agctactgga cattatgcgc aagtcactcg cgatgaaaac
     9061 ggcgtggtcc acatgcttcg tggagtagac aataataaag atcaaactta cttccttagc
     9121 caattatcac aagaacagct ttcaaaaacg atgttccctc taggcggcat ggaaaaatca
     9181 gaagtacggg caattgctga acgcgcaggc cttgcgactg ctaagaaaaa agattcaacg
     9241 ggaatctgct ttattggtga aaagaacttc aaacaatttt tgagcaacta tctgccagca
     9301 aaaaaaggaa atatggtcac tcttgatggt gaagtcaaag gacaacatgc tggcttgatg
     9361 tattatacga ttggtcaacg tcaaggacta gggatcggtg gcggaggaga ttcgcaagaa
     9421 ccctggtttg ttgtaggaaa agatctagcg acaaacacat tatatgtggg gcaagggttc
     9481 catcatcctg cattgtatgc cacaagcttg gatgcaagtg aaatccactt tacgacgaat
     9541 gaaccaatgc caaaagaatt caagtgtaca gcgaaattcc gttatcgtca gcaagacgtt
     9601 cctgtgacag ttcgcttatt ggatgataat cgtgcagaag tcgtattcga cgaacctgta
     9661 cgtgcaatca ctcctggaca agcagttgta ttttatgatg gaatggaatg tctgggtggt
     9721 ggtttgatcg accacgcata tcaagaaaca aaagttttac aatacgttta agcgatatta
     9781 aatgaatcaa aacagctatg gctaacaaaa gaaacacctt ttcttttgga tagcatagct
     9841 gtttttttgc aataaaatta atttttattc attttatact tgaaatactt gtgtaaaaag
     9901 ttcataatat ccaataatat gaataaagtt gctttttgtt aagcaagagc agttgcaaaa
     9961 tagaagggaa tcttttgcaa gtgatttgtt caattttata agtgagggtg atggaaatgg
    10021 aaaagaaatc gacgggtatt agcaaatggg gactggttgc tctggttgtc agttcgtcaa
    10081 tcggaagcgg tgtgtttggg atcacaagtg atctggctag ttctgcagct ccagggccag
    10141 cgatcctttc atggataatc gttgggattg gtattttagc tttggtatta tcgctgaatc
    10201 atttaggaga aaagcgtcca gatctagatg gtggtatttt cggttacgcg gaagcttcat
    10261 ttggtaaatt aggtggcttc atcagtggtt ggggttactg gttgtctgcg tggctgggaa
    10321 atgttgcttt tgcaacgatg ctgatgagcg ccatgggaga atttttcccg atattcaaag
    10381 gtggacaaaa tgttccttct attttatttg caagtatttt tatttggggt ctgactttat
    10441 tagtaaacaa tggagttgaa ggagcgagcg tcatcaatac aatcgtgacg atctgtaaat
    10501 tagtgccatt attcctattt ttaatatttg tatttatcgc atttaaagtg aatatattca
    10561 ctgctgattt ttggggtaac gtatctgata atctagtgaa tggaacaggg cagcaaggaa
    10621 cgatctggtt gcagattaaa ggttgtttga tggttatgat gtgggtcttt gtagggatcg
    10681 aaggcgcaag tgttttagcc aaccgcgcga agaaacgttc agaagcccag caagcatcga
    10741 tcattggtct gctttgttta ttgtttatct atatccttgc ttcgatcttg ccatacggcg
    10801 tcttatctca agaagagtta gcaactattt cacaacctgc tatggcaaat attttaaaag
    10861 gaatcgtggg aaattgggga gcagcgctga tcaatatcgg cttgattatt tcgatcattg
    10921 gttcttggtt gtcttggacg atgctcccag cagaaacaac gatgttgatg gctcgagacg
    10981 gtctgttgcc gaaaggttgg ggaaaaacga ataaaaaaaa tgccccaact tattcgttag
    11041 ttgttactgc aatcttgact aatctattct tattgacttt cttagttact gattatgcct
    11101 atcaatttgc ctattcttta tgtacagcgg caattttgat ttgttatcta cttgtcggga
    11161 tctatcaagt ccagttttct ttaaaagaag gaatcaaaaa acaagtagtg atcggtggga
    11221 ttgctgtagt ttttgaattg atgggcatca ttttagcagg attttcttat gtccttttat
    11281 gtagtattgc ctatcttcct ggtttttgct tctactggat cgcttgtcgt gaaaccaaac
    11341 atcaaattac gggaaaagaa aaagtaatga ttgggctgat ttgtgtggcc gcactgcttt
    11401 caatcgggtt gcttgctgta ggctggatca agatataagg gggaaagaaa catgaaaatc
    11461 gcaaatttcg gtgtagaaga atggctaaat aaatgggaaa agaaagcggt atatgatatt
    11521 gcacaaagct cgatcgaagc attgacatta gacgaactcg ttggcttaga tgggactagc
    11581 gtaagtaaat ttttcgaaaa acgcaaaaac gaaccattgg actatggctg gatcgaagga
    11641 tcagatgttt tcaaacaaga agtcgcttct ctttatcaaa ccgtcgatcc ggaaaacatc
    11701 ttacaaacga atggagcgac tggtgctaat cttttagctc tatacgcgtt agtggaagca
    11761 ggagaccatg ttgtttcgat gcttcctact tatcagcaat tatatgacat ccccaaatct
    11821 ttaggtgccg ctgttgactt tgttcatttg aaggaagaag aagattggca gtttgattta
    11881 gaacagctag aagccttagt caaacctgaa acaaaaatca tctgtttgaa tagtgcaaac
    11941 aatcctacag gcacattgct agatcggaaa actttagaaa aaatcgcaga aattgctcgt
    12001 acagtagatg catatgtatt gatagatgaa gtatatgcgc ctttaacgga taaaggtgaa
    12061 ttcttgtcga tcgtagatgt ctatgaaaaa ggtatagcaa ccaattccct atccaaaacc
    12121 tactccattc caggtattcg tattggctgg acagctactg gacctgagtt ggcagaagtt
    12181 tttcgaaagt atcgagacta cacgatgatt tgcggcggag tcctgtcaga tgatcttgct
    12241 gtacatgcat tgaagaataa agaaaaaata ttggcgcgaa acaaaaagat cattacagag
    12301 aaccttgtga ttttaaaaca atgggtagcc aatgaaccga aagttgaact tgtcgctccc
    12361 aattacgtct ctacttcttt cattaaatta gccatcgaag aagatgacca gacattttgt
    12421 atcaatttat tagaagaaac aggagtacta ttagtaccag gaagtgcctt tgatttaccg
    12481 aagcatgccc gactaggtta ctgttgtaaa aaagaaacct tggaaaaggg attattcctc
    12541 ttatctgagt tcttgaaaaa acaggcctaa acaaaaacac tgcaaggaca gtctgtgaca
    12601 actgttcaag cagtgttttt catttacgct tattgtctat gagaaggaga gtgccctgaa
    12661 gctatggaga ctcctgaagc tttttcttct gcagtttctt gctgatggat ctcttgtgta
    12721 ttgtgtcgta caagcaagaa gttgaccaaa acaagaagag aagtcgaaag aaagacgccg
    12781 cgataaccaa atgcactgga aacactggag ccgatcatag gccctactac gttaccagtt
    12841 gcttggaaag attggttata actgaagatc cgtccagcag cttgatgagg cgaatatttt
    12901 gtgatcaatg cttggacagc agggagcaga caagcatcag agataccaat caaaaaacgt
    12961 aaggcagcta attgccaaac attcgtgacg aatgccattg ggatataaac cagaatcgca
    13021 aaacccaaac caatcgcaag tattctttca ctcccaatct tgtcacctaa gcgaccaaat
    13081 cttggagcgg ctatcaaagt ggcaatccca ggtatcgaag caatgattcc gctgatcaac
    13141 gtaacattcc catgtccatg cagcaattgg cgaatgtaca gactgatgat tggactaatg
    13201 gaattgttgg atgcttggat gatcatcgtt gtgacgaaca taccgatgac gacgtgtgga
    13261 tatttcaagt ctttgaaaat ctgtttggca gaaaccatgt tttctttttt cactgggaca
    13321 aatttttcat ggacgaaaaa cagactcaac aagaagacga aaaataagat gatccctgta
    13381 atgaaaaagg tagggcgata gccaaacgct gaagcagaga tcccgccaag aagcggtcca
    13441 agtaatgttc ctgtaacaga accagttgct aaggttccaa gaacttgacc acttttttct
    13501 tttggcgtcc cagttgcgac aagggcagtg gcattactga tataaccaga aaaaatccct
    13561 tgaagcagac gcaatgcgac taattgatac acactagtca cgcaacccat caaagaaatg
    13621 acgattgcca ttcctaatga agcacgcaac agcatcaatt ttcttccttt ttggtcggct
    13681 aattttcccc accaaggaga gataagcgta gtcaccaaga aagtagatga aaaagtcaat
    13741 ccgctccaaa agttcaattg tgacgtacta taattaccta acgtatctat gtaaagtgac
    13801 ataaaaggca tgactaaact aaatccgata ccagccataa atgttccaaa ccataagaca
    13861 aacaagtttt gttcccaagt ttctcttttc ctaaataatt tttcctttat tcttgcagcc
    13921 aattagactc cttctttctg aaaattttca tctacagctc tttattataa actacaatat
    13981 gtagtttatc aatgaaagtc tcttttttct ctttcttttt cgtggtaaac tgaaagagtg
    14041 aaggatttat tttatgagag gaaacatgga aatgaaaaaa agaaatctgt ctcaagagaa
    14101 aatcatcgac tgctttcgag aactagcaga agagatggat gttcagcaaa taacctttca
    14161 gcatttagcc aaacgcttgg atatcaaggc tccctcgctg tataaccatt tcaaaaatat
    14221 tcgtgatgtc aaaacagcgc taacagcgaa acttctgcag gaacttaatg accagctgcg
    14281 acgggctctt gtgggaaaaa gtggtcagga agcactgcag atctatgcag aaacttatcg
    14341 atcctttgct ttttcaaatc aagcagtgta tgagttactg atcagtgtac cgcataccaa
    14401 tgaagctgtt ttattagatg gaatccatga aacgaatcag atcattttgc agatatttga
    14461 ggcttttcaa ttaacgaaag aagaaaaagt tcatcgaagc agggaacttc gtagtatgat
    14521 ccacggttat ctttccttac gttttttagg atactttaca aaagaaccaa caatcgatcc
    14581 agaagacagc tatttttgga tgatcaataa ctttatcgct actttgcctt aaacaatcag
    14641 attcatttgt aacaatttta agatgttcat taaatgaccc ttttataaga gaaaattctt
    14701 gcaactttct tgaaaagtga ctaagatgag tctagaaaag ctgaaagaag gagcacgagt
    14761 atgtatcaag taattaccat gttcggggac aatgagcctt ggtggttttt tgaagactgg
    14821 gaagaagata ttgaagaaga agaaacattc gagtctttcg aagaagcacg acaagcgtac
    14881 gaaaaacaat ggaacgaaat ccatcaaaat tatgaatata tcaatgcaaa aagtaacttt
    14941 ctcagcgcct tttggaatga cggagatgag cgctggtgtg aagagtgtga cgatgattta
    15001 cagcaataca agggacttgc gttgttgaaa aatcatcagc ccatcacagt agaaagcaga
    15061 aaggaatttt atgaaacaac taattctagc ggaaaaacca agcgttgcga aagacctaag
    15121 caaggtgctt ggtgctaatc aaaagcacaa aaattattat gaaggaccca aggtgatcgt
    15181 tacttgggcc cttggtcatt tattaggatt gaaaatgccg gaagatctga ataaggaatg
    15241 gcagagctgg caaatggaca cgttgccaat gatccctaaa aaactaggaa tcaaaccact
    15301 tcctaaaaca ggacatcaat taaaagcaat caagcagctg gctcagcgaa aagatgtctc
    15361 agaagcagtc attgcgacag atgccggaag agaaggggaa cttgttgctc gctggatctt
    15421 agagtatgtg catttcaata aaccagtgaa gcggttatgg atctcttctc aaacagataa
    15481 agcaatcaaa actggattca agcaactaag acctgcgaaa gaatatgatg cattgtatga
    15541 atctgctctt gcccgggcaa aagcggactg gctggtagga ttgaatgtga ccagagcctt
    15601 aactgtgaag tacgcggata atttatcagc aggccgtgtg cagacaccga cactagcatt
    15661 agtgagagac caagaaaaga aaatcgagca gttccgtcca caaacgtatt tcacgatcac
    15721 gttgcaagct ggaaaagaac aagccaaaat gcttcaaaaa aatcaatttg ctttaaaaac
    15781 acaagaagaa gcacaaaaac ttgttcaatt gatcagtaca agcaaaggaa ctgtcacaga
    15841 agtccaagaa aaattaaaaa cggaatctgc gccattgcct tatgatttaa cagagatcca
    15901 aagagaagcc aatcaacgct atggctattc tgcaaagaaa acattaagtc ttgtacaaag
    15961 tttgtatgag acccataaga tcgttactta tccgcgtaca gacagcaaat atctgacaaa
    16021 tgacatgaaa agtacgatga aagaacgatt gcaagcagtc agcgactttg ctcctgaggt
    16081 caagcagtat ttgaaaaacg gagccatcgt ccaacagaca aaagtattcc aagacagtaa
    16141 agtgaccgat caccatgcac ttttgccaac tgaaaacaga gcacgttatg aaaaattaag
    16201 taatgaagag cgaaaaatct atcaaatgat tgtctctcgt tttttaggac tgtttgctca
    16261 accccataaa gtcagccaaa caaaagttac ggttgaattt ggtaaagaac gattcatttt
    16321 tcggcaaaat cgtgtcgtcc aagcaggatg gaaaggcgaa actgaaactg agacggaaac
    16381 agtcaaatgg gaaaaaggaa tgagaatcaa tcccgatttc gccatcaaaa aagaactatc
    16441 tgcaccgcct aaaccattgt cagaagcaag cttgcttggt caaatggaaa aatacagtct
    16501 gggaacccca gctacgcgtg cagagattat cgagaaacta atcaaatctg aactgatgga
    16561 acgtacccct caaggactgc aagtatctcc taaaggtaag caattattag tattggtaaa
    16621 tccctcttta gtaacaccag atttgacaga aagctgggaa aaagaccttg aagctattgc
    16681 tgcaggaaag aaacaagcag gaaccttctt gaatggaatc gaaaaagaaa caaaacgctt
    16741 ggtgaatgag atcaaatcaa gcaaacaaga atatcaagat ttctcgatta cccaaaagaa
    16801 atgcccagaa tgtggggcca atctgcgcga gaaaaacaca cgtgacggca aaatctatgt
    16861 ctgcaccaat tcagattgca actaccgcag aagaaaagat ccaaaagtct ctaatcatcg
    16921 atgcccgcaa tgtcaccgga aaatggaaat catcgaaggt aaaaacggtg cttatttccg
    16981 ttgtaaatac gatggaacga cagaaaaatt gcttgataaa aaagagcaga agaagaaaat
    17041 gaccaaacat gaagaacgac gcttgctgaa aaaatatgca caagcagaag aaccagaaga
    17101 aagcccatta gcagcagcac taaaagcagc aatgaagaag taataagata tgaaagaggt
    17161 tgtga
//
LOCUS       DACCSK010000055        17004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCSK010000055 DACCSK010000000
VERSION     DACCSK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.056"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..124)
                     /locus_tag="IVP39_001604"
     CDS             complement(<1..124)
                     /locus_tag="IVP39_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP7586679.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..518)
                     /locus_tag="IVP39_001605"
     CDS             complement(228..518)
                     /locus_tag="IVP39_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7586680.1"
                     /translation="MVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYELDPSPLI
                     NRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            complement(540..737)
                     /locus_tag="IVP39_001606"
     CDS             complement(540..737)
                     /locus_tag="IVP39_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7586681.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1131..2858)
                     /locus_tag="IVP39_001607"
     CDS             complement(1131..2858)
                     /locus_tag="IVP39_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7586682.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2851..4044)
                     /locus_tag="IVP39_001608"
     CDS             complement(2851..4044)
                     /locus_tag="IVP39_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7586683.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4231..4875)
                     /locus_tag="IVP39_001609"
     CDS             complement(4231..4875)
                     /locus_tag="IVP39_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7586684.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4969..5103)
                     /locus_tag="IVP39_001610"
     CDS             complement(4969..5103)
                     /locus_tag="IVP39_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7586685.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5105..7237)
                     /gene="feoB"
                     /locus_tag="IVP39_001611"
     CDS             complement(5105..7237)
                     /gene="feoB"
                     /locus_tag="IVP39_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7586686.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7234..7707)
                     /locus_tag="IVP39_001612"
     CDS             complement(7234..7707)
                     /locus_tag="IVP39_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7586687.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8108..8332
                     /gene="nrdH"
                     /locus_tag="IVP39_001613"
     CDS             8108..8332
                     /gene="nrdH"
                     /locus_tag="IVP39_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7586688.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8491..10650
                     /gene="nrdE"
                     /locus_tag="IVP39_001614"
     CDS             8491..10650
                     /gene="nrdE"
                     /locus_tag="IVP39_001614"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7586689.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10700..11665
                     /gene="nrdF"
                     /locus_tag="IVP39_001615"
     CDS             10700..11665
                     /gene="nrdF"
                     /locus_tag="IVP39_001615"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7586690.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11754..12893)
                     /locus_tag="IVP39_001616"
     CDS             complement(11754..12893)
                     /locus_tag="IVP39_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7586691.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13202..13915)
                     /locus_tag="IVP39_001617"
     CDS             complement(13202..13915)
                     /locus_tag="IVP39_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7586692.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14169..14870)
                     /gene="nagB"
                     /locus_tag="IVP39_001618"
     CDS             complement(14169..14870)
                     /gene="nagB"
                     /locus_tag="IVP39_001618"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7586693.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15104..16636
                     /locus_tag="IVP39_001619"
     CDS             15104..16636
                     /locus_tag="IVP39_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7586694.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16827..>17004)
                     /locus_tag="IVP39_001620"
     CDS             complement(16827..>17004)
                     /locus_tag="IVP39_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7586695.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5322 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt tttttcattt
      541 tagcacttcc ctggaattgc tccacctaaa aaaccaccta tagacattcc tgcaatacaa
      601 gtagttgcct tggcccaatc gaccgtacat ttatttttag tgcaatacac tccatttcca
      661 taatattttc cactatgagt ggtaccccca tagataagtt gtgtctcttt aatagacaaa
      721 atttttaaat gtttcataat ctaatcaccc ctaaattaat gtttttagac tattattcta
      781 acattcatta aatttcaaaa aacaattcct tcttgaaacg tagcaaaata attcctgaat
      841 aagaatttaa tcacttttaa gtatataaac tatgtatatt ctttaatatt ttttgagaat
      901 gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt
      961 tgactcgcac ccataaattc ataatagttt gtcggcgtct ttgaacgttt cgaagatgag
     1021 ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt
     1081 tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc
     1141 cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct
     1201 tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg
     1261 tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg
     1321 atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg
     1381 gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct
     1441 tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag
     1501 ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt
     1561 cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag
     1621 gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga
     1681 tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca
     1741 tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt
     1801 acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta
     1861 tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct
     1921 ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt
     1981 ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt
     2041 gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc
     2101 ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc
     2161 atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg
     2221 atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg
     2281 aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa
     2341 cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata
     2401 ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca
     2461 aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt
     2521 accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa
     2581 tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt
     2641 cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga
     2701 gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt
     2761 aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag
     2821 tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc
     2881 tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac
     2941 aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc
     3001 tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct
     3061 agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc
     3121 catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga
     3181 ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg
     3241 atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc
     3301 acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg
     3361 taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag
     3421 gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc
     3481 gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg
     3541 catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc
     3601 taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc
     3661 gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat
     3721 attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc
     3781 tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa
     3841 tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc
     3901 aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg
     3961 ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa
     4021 atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt
     4081 tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga
     4141 aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc
     4201 aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa
     4261 aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc
     4321 cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat
     4381 aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat
     4441 tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt
     4501 cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt
     4561 ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg
     4621 caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac
     4681 ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc
     4741 attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt
     4801 ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt
     4861 tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct
     4921 ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat
     4981 ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa
     5041 acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc
     5101 catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct
     5161 gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg
     5221 ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta actaaataag
     5281 ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat
     5341 ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat
     5401 aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg
     5461 atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca
     5521 atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa
     5581 ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat
     5641 aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg
     5701 cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag
     5761 gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg
     5821 tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag
     5881 gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact
     5941 ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt
     6001 caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa
     6061 tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc
     6121 tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg
     6181 gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca
     6241 tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac
     6301 caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat
     6361 aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa
     6421 tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg
     6481 tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt
     6541 ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta
     6601 tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt
     6661 tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg
     6721 gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt
     6781 cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa
     6841 gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg acatccatca
     6901 tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat
     6961 ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca
     7021 aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta
     7081 ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta
     7141 cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt
     7201 tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa
     7261 gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat
     7321 tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat
     7381 atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc
     7441 tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac
     7501 aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat
     7561 ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt
     7621 ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt
     7681 ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat
     7741 taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat
     7801 tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg
     7861 aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat
     7921 tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa
     7981 aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga
     8041 cttttttact aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc
     8101 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
     8161 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
     8221 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
     8281 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
     8341 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
     8401 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
     8461 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
     8521 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
     8581 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
     8641 ccatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
     8701 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
     8761 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
     8821 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
     8881 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
     8941 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
     9001 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
     9061 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
     9121 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
     9181 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
     9241 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
     9301 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
     9361 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
     9421 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
     9481 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
     9541 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
     9601 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
     9661 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacagatat ctcatgtaac
     9721 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
     9781 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
     9841 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
     9901 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
     9961 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
    10021 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
    10081 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
    10141 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
    10201 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
    10261 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
    10321 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
    10381 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa cggcacaaca acacgtcgat
    10441 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
    10501 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
    10561 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
    10621 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
    10681 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
    10741 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
    10801 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
    10861 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
    10921 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
    10981 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
    11041 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
    11101 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
    11161 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
    11221 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
    11281 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
    11341 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
    11401 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
    11461 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
    11521 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
    11581 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
    11641 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
    11701 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
    11761 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
    11821 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
    11881 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
    11941 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
    12001 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
    12061 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
    12121 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
    12181 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
    12241 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
    12301 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
    12361 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
    12421 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
    12481 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
    12541 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
    12601 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
    12661 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
    12721 aacatataat agataaaacc agcaacaagc aatggtaaaa aaattgccga aagcattttc
    12781 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
    12841 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
    12901 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
    12961 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
    13021 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
    13081 cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact
    13141 atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca
    13201 tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct
    13261 cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca
    13321 ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct
    13381 cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta
    13441 actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc
    13501 cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag
    13561 caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga
    13621 tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat
    13681 ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag
    13741 cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac
    13801 gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca
    13861 tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa
    13921 caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac
    13981 ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg
    14041 tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt
    14101 tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact
    14161 attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga
    14221 ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt
    14281 gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa
    14341 ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg
    14401 tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca
    14461 ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt
    14521 ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt
    14581 ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa
    14641 tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa
    14701 gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg
    14761 cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca
    14821 aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct
    14881 cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt
    14941 atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    15001 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    15061 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    15121 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    15181 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    15241 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    15301 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    15361 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    15421 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    15481 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    15541 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    15601 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    15661 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    15721 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    15781 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    15841 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    15901 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    15961 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    16021 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    16081 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    16141 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
    16201 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    16261 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    16321 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    16381 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    16441 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    16501 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    16561 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    16621 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    16681 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    16741 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    16801 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    16861 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    16921 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    16981 ataaagtaca taaacataaa taca
//
LOCUS       DACCSK010000056        16974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCSK010000056 DACCSK010000000
VERSION     DACCSK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.057"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            125..1168
                     /locus_tag="IVP39_001621"
     CDS             125..1168
                     /locus_tag="IVP39_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7586696.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1230..1694)
                     /locus_tag="IVP39_001622"
     CDS             complement(1230..1694)
                     /locus_tag="IVP39_001622"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7586697.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1940..2887
                     /locus_tag="IVP39_001623"
     CDS             1940..2887
                     /locus_tag="IVP39_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7586698.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3264..4550)
                     /locus_tag="IVP39_001624"
     CDS             complement(3264..4550)
                     /locus_tag="IVP39_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7586699.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(4543..5811)
                     /locus_tag="IVP39_001625"
     CDS             complement(4543..5811)
                     /locus_tag="IVP39_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7586700.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            6063..6524
                     /gene="mscL"
                     /locus_tag="IVP39_001626"
     CDS             6063..6524
                     /gene="mscL"
                     /locus_tag="IVP39_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7586701.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(6583..7029)
                     /locus_tag="IVP39_001627"
     CDS             complement(6583..7029)
                     /locus_tag="IVP39_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7586702.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(7122..7367)
                     /locus_tag="IVP39_001628"
     CDS             complement(7122..7367)
                     /locus_tag="IVP39_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586703.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(7482..8060)
                     /locus_tag="IVP39_001629"
     CDS             complement(7482..8060)
                     /locus_tag="IVP39_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7586704.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(8137..8667)
                     /locus_tag="IVP39_001630"
     CDS             complement(8137..8667)
                     /locus_tag="IVP39_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7586705.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(8671..9531)
                     /gene="rlmB"
                     /locus_tag="IVP39_001631"
     CDS             complement(8671..9531)
                     /gene="rlmB"
                     /locus_tag="IVP39_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7586706.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(9524..9919)
                     /locus_tag="IVP39_001632"
     CDS             complement(9524..9919)
                     /locus_tag="IVP39_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7586707.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(9916..11328)
                     /locus_tag="IVP39_001633"
     CDS             complement(9916..11328)
                     /locus_tag="IVP39_001633"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7586708.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(11325..11861)
                     /gene="cysE"
                     /locus_tag="IVP39_001634"
     CDS             complement(11325..11861)
                     /gene="cysE"
                     /locus_tag="IVP39_001634"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7586709.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(12183..13643)
                     /locus_tag="IVP39_001635"
     CDS             complement(12183..13643)
                     /locus_tag="IVP39_001635"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7586710.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(13813..14970)
                     /locus_tag="IVP39_001636"
     CDS             complement(13813..14970)
                     /locus_tag="IVP39_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7586711.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(15004..16374)
                     /gene="radA"
                     /locus_tag="IVP39_001637"
     CDS             complement(15004..16374)
                     /gene="radA"
                     /locus_tag="IVP39_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7586712.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(16437..16964)
                     /locus_tag="IVP39_001638"
     CDS             complement(16437..16964)
                     /locus_tag="IVP39_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7586713.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         4512 a         3622 c         3096 g         5744 t
ORIGIN      
        1 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
       61 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
      121 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
      181 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
      241 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
      301 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
      361 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
      421 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
      481 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
      541 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
      601 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
      661 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
      721 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
      781 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
      841 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
      901 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
      961 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
     1021 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
     1081 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
     1141 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
     1201 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
     1261 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
     1321 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
     1381 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
     1441 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
     1501 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
     1561 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
     1621 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
     1681 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
     1741 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
     1801 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
     1861 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
     1921 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
     1981 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
     2041 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
     2101 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
     2161 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
     2221 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     2281 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     2341 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     2401 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     2461 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     2521 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     2581 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     2641 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     2701 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     2761 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     2821 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     2881 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     2941 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     3001 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     3061 agctgaccac ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag
     3121 ttgattaaaa gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag
     3181 aataccataa aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag
     3241 acttttcctc ataaaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct
     3301 tcacttataa atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt
     3361 tcaggaagat caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata
     3421 tattcgatcg aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg
     3481 agatttgttt ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt
     3541 tctgctgctt tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga
     3601 tccaagctga attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga
     3661 tagtttcgag aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag
     3721 cgtaacagtt ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat
     3781 ttatctcgtg taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca
     3841 gggaaataac gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga
     3901 ataagtttca tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt
     3961 ggctgataaa acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt
     4021 tcaaccgtgc ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg
     4081 ccaaaaaaca tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt
     4141 cctttttcct tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga
     4201 gtcgtcagat tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa
     4261 cttgtgaaag cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct
     4321 tctttttcaa acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt
     4381 tctttttcac catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa
     4441 ccatatgtct tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt
     4501 acttcatgat aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc
     4561 tcctgctaaa aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt
     4621 gatcgttacg ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag
     4681 ccagctgtcc aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata
     4741 ttggtttctc agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg
     4801 caaactttct aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat
     4861 accggtttgg acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc
     4921 tagactttct ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa
     4981 accgttaaag accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata
     5041 agctaggttc agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt
     5101 cacttgaggt tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa
     5161 ttgcccaact gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat
     5221 ttgatcattt gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc
     5281 caaatcttcg actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag
     5341 ttcttgcaag gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag
     5401 acgcagattg ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt
     5461 tggataaaat aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt
     5521 gacatattca ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt
     5581 tctaactgat aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt
     5641 ctgatccgga taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt
     5701 gatgacttct tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg
     5761 ttttgttggt aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg
     5821 ctccttttca cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact
     5881 tattcctctg cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta
     5941 cactttatag tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata
     6001 ttgttttttt attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat
     6061 tcatgatcaa agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg
     6121 gcgttgttat tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca
     6181 caccactaat aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac
     6241 tttcaattct tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta
     6301 gtgctatcat tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa
     6361 accgtgcgaa gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg
     6421 cagaagatta tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata
     6481 cacatccagt agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa
     6541 aagaaagccg attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc
     6601 ctttttcatg tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa
     6661 tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc
     6721 tggaaaattt ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc
     6781 tttacggttg tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata
     6841 tactcgcgtg taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttg
     6901 tccatcaatg tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat
     6961 tcgttctttg acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg
     7021 atattccact aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa
     7081 aaaagcaaac gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa
     7141 aattcgatct caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa
     7201 gaattttctt caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg
     7261 ccgcggcgtt ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg
     7321 atacgacctt ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct
     7381 actatcaact ataccacata tttttaaaat tttcaagttt accataaaaa aaaaagaaat
     7441 gcaataaaaa taaataagaa gctcattaag atttttctaa attattcctc attataaaat
     7501 ttcaagtgtt tttttatttt gagttttact ctcccgtaag cgctcattac ttgcttgtga
     7561 tccattgcta aacattcagc catgtctaca aaatcaacgc cttgcatata ataatgaaaa
     7621 attttagctt ctatttttga aaactggatt tcgcaatttt cgattgcttc ttttaataaa
     7681 agatgctgaa tcgctaaagg ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct
     7741 gtctctattt tttggtcgat tgagacttca tctttttgat cacgacgctt cagcgcgttc
     7801 tgcttcctca acagagaata gatttgattg cttataatgc ttttataata acttccaaag
     7861 ctaacacctt tgcttttttc gtaagttctc aatgcctgat ataaagcgat ccttgcttct
     7921 tgttcccaat cctcagaaac ataatttctt aaataatagg tgtttttcaa acgtagtaaa
     7981 agtggaaaat atttttgata cagggcagca aatgcttctt cgtcaccatt acgtgcagcg
     8041 cgcaataact cctcagacat aaaataattc ctccataaat tcgtttatgg aggaatctta
     8101 tcatcaatga ttcatcgaac tgattagaac gatatgttat ttattttttc tcattaaatc
     8161 gtccaatttt tgggacaatt tttttaactg ctcttcattc catggagaat tacgtcggaa
     8221 atcaataaaa tgcatatctt tattttcttg ggctattgtt ttttccgttt tttttatcgt
     8281 cttatataat tcataagcag aagttcgtaa tgctccttgg gaaaagatca tccactgttc
     8341 tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat
     8401 tctctcgata taactgtcag ccgtttcgtc ttctttcgta aaaacaacat ccaactggta
     8461 tttcttataa ttttgttgga tgcccggaac tagttgggca tcgaagacaa cgatgacttc
     8521 cattccttca tatttcgcat aatttgataa acgatgtaaa agagcttctc ttgcatccgc
     8581 cattttgtcc tgctttttaa gctggacaag ttccggccac gaaccaatca tgttgtagcc
     8641 gtctacaatc aacagctgtt ttttcatggt ctactccccc tgacgttggg aaaaagcctg
     8701 atacataagc aaaccagcag caacactggc atttaagctc tggacgtgcc cggtcatcgg
     8761 gatcgtcagc aattcatcca tttctttttt caatcctgga ctcattcctt tgccttcatt
     8821 accaatgact aaagcaattg atcctttggc attccatttt tggaagtttg tgccggtcat
     8881 atctgtgcca aatacccaaa atcctgtttc tttgagctgt tgcacagctt gtaccaaatt
     8941 cgtcacgcgt gcaacaggga catgttcgac tgccccagtg gaagtctttg ttacgatagg
     9001 tgtgattcca actgctcggt gttttggtat gatcacacca tcgacgccac ttgcgtctgc
     9061 tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa
     9121 ggggtcttct tttttctctt ttgttcgttc aatcagttgg tcaagggata aatattcata
     9181 aggtgtaatc gcaagaacga ctccttgatg cacgccgtga tcgctcaaag tttctaattt
     9241 ttgtttagga acccatttga ctggcacagc ttgttcacgt gcgatttctt tcagttcttc
     9301 tactttttct cctcgtgaat cttcttgaag aaagagttta ttccctcgcc cttgttgtag
     9361 cgcttctact gcggcatggt gcccaaagac gaaattatct tctaatgctt cttcattttg
     9421 atcagaaact gactgatttc gtccagtttg ctttttattt gaatatctat tttgtttttt
     9481 gtcttcaaat ccacgtttcg attgatgttt gtttgttctt tctttaccca tttttttcac
     9541 caactttctg aatacaccag cggatcaatt cttccatccg ctcggtttgt tttgtcaaat
     9601 gcagatagcc catgagcgct tcgaaccctg ttgcgacccg atacgtcgta acatcagcat
     9661 tttttgcgct cgtatggctt ttagcatttc ttcctctgcg ataataaagt tcttcttctt
     9721 ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa
     9781 cataattcgt tgccgcacga tgcagcttat tgggttttgt ttctccttga tcaatcaggt
     9841 ggtcacgaac atagatttcg taaatcgcat ctccaacata agccaaggct aaaccgttca
     9901 attgtgtgta atctctcatg cttctcttct ccatcgggtt ccttgcggtg tatcttctaa
     9961 taagatccct ttttctttca acaaatcacg aatttcgtcg cttcggacaa aatcacgatt
    10021 gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta ataactcttc
    10081 tgctgtaaat aaaataccga agatcgaaag ccattgcgag aataaagctt ctccttgtgc
    10141 caagacagcg gcagaaactt tcggctggtc tagataagtg ttcagccact tagcaagctc
    10201 atagaccact gtgatcccat ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg
    10261 ctgttctaaa ttttctaaca aagcgatttt atccgcatca tctgccaaat tctctgaggc
    10321 agtttcttgg cggaatttca aattgtcaaa cgtattcttc agtcgttgta aattggtggc
    10381 tgcttctttc atcgtcgttt cactgtagcg gatcggacga cggtattgtg tggtcgccat
    10441 gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt
    10501 ccctagagat ttactcattt tttcatcgtc ttcgccaatc gttacatagc cattgtgcat
    10561 ccagtaatta gcgaattttt tccctgtttt cgcttcactt tgtgcgattt cattttcatg
    10621 atgaggaaat tctaaatctt gcccgccacc atggatatcg atcgtttctc ctaaatgttt
    10681 tgttgccata acggaacatt caatatgcca accgggacgt cctttgcccc atggtgaatc
    10741 ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt
    10801 ttgctgctgt tcttcccctg tacgctgact tgctccgact tccagttcat cgatggattg
    10861 gtcacttaat ttcccataat tttcaaattt acgggtacgg taatacacat cacctgaaga
    10921 ttcataagca tagccttttt caaccaacac ttgaataaat gctaaaatat ctggcatatg
    10981 atccatgact cttggatgca gtgtagcagg ctcgacattc aacgctttcg tatcttcttc
    11041 aaatgctcgg atgaaacgat cagcaacttc tggtgctgta atacctaatt cgtttgctgt
    11101 acggatgatt ttatcgtcta catccgtgaa attcgatacg aaatcgactt tatagcctcg
    11161 atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg
    11221 gatataatta tacacagtcg gtccgcagac atacataccg acatgtccct tttgtatagg
    11281 gatgaattct tctttttttc tggtcaatgt attgtagatt tttatcattc tgatctcctc
    11341 actttttctc ctttgattcg aacgactttt gctggtactc ctaccgctgt agcgtcgtct
    11401 ggaacatctt tcaacacaac ggcacctgca ccgattttgg cgttttttcc gatcgttacc
    11461 ggtcctaata tctgcgcatt cgcagagagc atggctcctt gtttaacagt tgggtgtctt
    11521 ttacccgttt cttttcctgt gccgcctaat gttacgccat ggaataaaat cacatcgtcc
    11581 tcgatttctg cggtttcgcc aatgacgatt cccattccat ggtcgatgaa cacgccagtt
    11641 ccaatcgttg ctcctggatg gatctcaaca ccagttagga aacgccagaa ttgagcattg
    11701 atcttcgcca ataaaaagag ccggtgctga tataaaaaat gagagaaacg atgccaaaac
    11761 aaggcatgaa aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg
    11821 tttctctttg ctgcatcaat cgctcgggta aaccaactca tgactttcca ctcctttcgt
    11881 aagaatttta tgtaggaaat catcttctta taaagaaaag aagcgccttt taaataagtt
    11941 tccttatcaa aagacgcttt ccgcgtggtt ccacttttct tcataatcaa tgattatctc
    12001 tgacagctaa cgcctgttat acgttcttgg ctactttgtt tcctataaaa cagtttttcc
    12061 tgttttctgt gtttcgccaa gaccactccc agaggcattt cggcaaccat tcattgatac
    12121 cttgcaccac ccggtatctc tcttgtaatg atcagctgct tacttttttc tgttcatcgt
    12181 gtttagttca gtacttgatt taaatgtgct aatgattttt cacgaccaag caattcgatc
    12241 gtttcaggca attctggacc atgcatttgt cctgatacag ctacacggat tggcatgaaa
    12301 agattttttc cttttacgcc agtttctttt tgaacagctt tgatggcagc tttgacagtt
    12361 ggagcatcta ctacttccat ttgttctagc tgtgctttga atgcgtggag aacagtagga
    12421 actgtttcac cagcaagtac ttcttttgct gcttcattca atacaggatg ttcattgaaa
    12481 aatagcgtag acagatcaac gatttctgca gcatagctca tttgaggttg atacaggcta
    12541 acgatcttct tcacccattc gattgtcttt tcatctgggt tttctgctac acgtccatca
    12601 gcgattagat aaggcagaca catgtcagta aatgcatcta gatccattgc tttgatgtaa
    12661 tggttgttta cccattgcag ttttttcgca tcgaaagctg cgggtgattt acttaggcgt
    12721 tttggatcga acattttgat caattcttgg tgttcgaaga tttcgtcttc gcccacaggt
    12781 gaccagccca ataatgcaat aaagttgaac attgcatctg gtagatagcc taattcgcga
    12841 tattgttcga taaattgcaa gattgattcg tcacgtttgc ttagtttttt acctgtttca
    12901 ctgttgatga tcaatgtcat atgtccgaag gttggtggtg tccagccaaa tgcttcatag
    12961 atcatcagtt gttttggtgt attggcaata tggtcatctc cacgtaaaac gtgagtgatt
    13021 ttcatcaaat gatcatcgac cgcaacagca aaattataag ttggcatacc atcgcgtttt
    13081 tgtatgacaa agtcgccacc gatattatcg gattcgaacg aaatagcgcc tttcaccata
    13141 tctgtaaatg cgtattctgt attgcgaggc acacggaaac gtacaacaga ttctagacct
    13201 tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga
    13261 ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa
    13321 tagcatttgt atgctcgatt gctagcaagc agctgatcaa tcagcggttg atagatttct
    13381 ttacgttcag attgacggta agggccgtat tctccaggat tttcaggaga ttcatcccaa
    13441 ttcattccca accaagcaag gttttccaac tggctttttt cgccatcttc gatattacgt
    13501 ttttggtcag tatcctcaat acgaataata aagtctccat cattgtggcg ggcaaacaaa
    13561 taattgaata atgcagttct tgcatttcca atatgcaggt gcccagttgg acttggtgca
    13621 tagcgtacgc gtactttcgt cattttcttc ctcttctttc aaactagact ttgactaaca
    13681 gcaaaaattc cgtcagttac ggcttctcaa atatcattta tttaaagcca ctcagcacaa
    13741 attttacctt ttctaaggta aatagtaaag ccaactggtt gaatttcctt accagttggc
    13801 tgaatttctt ttttattttg attgtttagt ctttttctct gtctcttttt tattgtcgat
    13861 tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc
    13921 acttgttaca acgacttcga tacgttcatt catgtaatgc tgtccatctt ctacgacaac
    13981 catcgtgccg tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg cttttactac
    14041 tagaacattc atcgtttcac cagggatgac gactggtttt acggcatttg ccaacgcatt
    14101 gatattaaga acaggtacat tttggaattc agagacttta ttcaagttat agtcattggt
    14161 tacaatgaca ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta cttcactgat
    14221 atcttcaaag tctccatcgt acatttcgac agatataccc tcttcttttt gcaaggcatt
    14281 taggatatct aatccgcgac gaccacgcac acgtttcaaa ctatcccctg agtccgcgat
    14341 gtattgtagt tcgtaaagaa caaaattagg gatcatcaag gttccttcta agaatccagt
    14401 ctttgtaata tcataaatcc gaccgtcaat gatcacgctt gtatccaaga ttttatattt
    14461 atggaaattt tctcctgatt ttcgttctaa cagttgactc tcaacttgac tagctgtttc
    14521 ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt
    14581 tcccattctc aatcccagat agcctaagat gatcatcagt aaaatcggca atacactatt
    14641 gacgaatgga atttccatat tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc
    14701 gatgatcgca ccgatcgcac cgaagagaag ataagtcaag ctgacctcac ttagtttttg
    14761 ttctacttgt ttgataatag ctgagatatg tttggccaac agcaaagata ataaaaagaa
    14821 aataagtgcg cctaacaggc tgttggtaaa tgtattattc agccatccgt ttgactgttg
    14881 accgattgcc tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat
    14941 catcagcaaa gtaatgacac gtttttgcat cgtttttcct ccgtttcgac aatcatttat
    15001 tagttagcgg aacacacgct tcaacgtttc agccaaagtt gctactccaa ctatctcgat
    15061 tccttttggc gcttcccagt tacctaagtt gtttttaggt aggtatactt ttgtgaagcc
    15121 gagtttttgg acttctttga ctcgttgttc aatatgactg acacgtctga tttctccagt
    15181 cagtccaatt tcaccgatga aacattccgt tggctgtgtt cctttttctt tatagcttga
    15241 agcaatactg acagcgatag ctaaatcaat cgctggttca tttaatttta ccccacctgc
    15301 agcttttaag taagcatctt gattctgaag aagcaaccca gctctttttt ccagcactgc
    15361 catgatcaga gatacccgat tgaaatcaag acctgttgtt gttcgtttgg catttccgaa
    15421 catagtaggt gtgactaatg cttggatctc taccaaaatt ggacgtgtcc cttccattgc
    15481 cacaacgata gcagagccag ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg
    15541 attagcgact tcttccaatc catgctcatg catttcaaaa atcccaatct cattcgttga
    15601 gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa
    15661 atataacacc gtatccacca tatgttctaa cattcttggg ccggcgatcg agccttcttt
    15721 ggtaacatga ccgacgataa aaatggctat cccatttgtt ttagcgattt tgagtagttc
    15781 tgcagtggtt tctcttactt ggctgacact tcctgccaca cttgtgatgt ctggctgcgt
    15841 catcgtttgg atggaatcaa tgataacata atcaggggag atatgttcga tggcacgact
    15901 gatttcgttc atatctgttt cagcgtatag ataaaattct gtgttgatac ttccgaggcg
    15961 ttccgcgcgt aattttatct gctctgcgct ttcttcgccg gatacatata agacttttcc
    16021 gcctatggct gctaattgct gagaaacttg taacagcaaa gtggatttcc cgatacctgg
    16081 atcgccacca atcaagacca tagaccctgg caccacacct ccgcctaaca cacggttcag
    16141 ttcttctaat tttgttttga tccgaggttc ttttttaggg acgacgtctg cgatttttgt
    16201 tggttttgct ttttctcctg tcaaacttgt ccgagttctg cggtccgttg tatcttgttc
    16261 gatttcttcg accatcgtat tccattttcc gcaatttggg cagcgtccca agaacttagg
    16321 cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg ccattgtacc
    16381 gcctcctttt atcctaatca tgagacatta aatcaagctt aagtatcttt aacgttttat
    16441 ttactggaag aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg atctgctttt
    16501 aaaaacggca aaaagatccc ttggccgata cgctcgcctt ttttgatcac tacatccttg
    16561 aaaccaaagt tggtgaattg gaacatgata tggccttcat ttgcctcatt attgtaataa
    16621 tcactgtcaa tcacacctac tccgtttgtc aacactaaaa accgcttcaa cggattgctt
    16681 gaacggttag caagctgcaa aaattcatct tctcccatgt aagctttgat tccagtaggc
    16741 acgagggttg gtttcaattg tttttcattt tctgatacat cttttttcaa tccatctgcg
    16801 actgtttcca accccttttt ccagcctgct ttccagatac ttggaatcac gacctcttct
    16861 gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc gctgcggtag attgatattt
    16921 tgatctttat atttagaaat aacttcaaat cctctttttc tcaccggttt tcct
//
LOCUS       DACCSK010000057        16847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCSK010000057 DACCSK010000000
VERSION     DACCSK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.058"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(31..1263)
                     /locus_tag="IVP39_001639"
     CDS             complement(31..1263)
                     /locus_tag="IVP39_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7586714.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1517..2086
                     /locus_tag="IVP39_001640"
     CDS             1517..2086
                     /locus_tag="IVP39_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7586715.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2264..2704)
                     /locus_tag="IVP39_001641"
     CDS             complement(2264..2704)
                     /locus_tag="IVP39_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7586716.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2862..3626
                     /gene="map"
                     /locus_tag="IVP39_001642"
     CDS             2862..3626
                     /gene="map"
                     /locus_tag="IVP39_001642"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7586717.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3658..4581
                     /locus_tag="IVP39_001643"
     CDS             3658..4581
                     /locus_tag="IVP39_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7586718.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4657..5796
                     /locus_tag="IVP39_001644"
     CDS             4657..5796
                     /locus_tag="IVP39_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7586719.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5789..6589
                     /locus_tag="IVP39_001645"
     CDS             5789..6589
                     /locus_tag="IVP39_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7586720.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6589..7416
                     /locus_tag="IVP39_001646"
     CDS             6589..7416
                     /locus_tag="IVP39_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7586721.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7394..8128
                     /locus_tag="IVP39_001647"
     CDS             7394..8128
                     /locus_tag="IVP39_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7586722.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8228..9094
                     /gene="rfbA"
                     /locus_tag="IVP39_001648"
     CDS             8228..9094
                     /gene="rfbA"
                     /locus_tag="IVP39_001648"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7586723.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9108..9680
                     /gene="rfbC"
                     /locus_tag="IVP39_001649"
     CDS             9108..9680
                     /gene="rfbC"
                     /locus_tag="IVP39_001649"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7586724.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9702..10730
                     /gene="rfbB"
                     /locus_tag="IVP39_001650"
     CDS             9702..10730
                     /gene="rfbB"
                     /locus_tag="IVP39_001650"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7586725.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10828..11679
                     /gene="rfbD"
                     /locus_tag="IVP39_001651"
     CDS             10828..11679
                     /gene="rfbD"
                     /locus_tag="IVP39_001651"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7586726.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11713..13746
                     /locus_tag="IVP39_001652"
     CDS             11713..13746
                     /locus_tag="IVP39_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586727.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13790..15055
                     /locus_tag="IVP39_001653"
     CDS             13790..15055
                     /locus_tag="IVP39_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7586728.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNHNSYNSILNIYGLIGFGIAALLLILFIGRLTLKAD
                     LSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEEFYVME
                     EAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15265..16071
                     /locus_tag="IVP39_001654"
     CDS             15265..16071
                     /locus_tag="IVP39_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586729.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16083..>16847
                     /locus_tag="IVP39_001655"
     CDS             16083..>16847
                     /locus_tag="IVP39_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586730.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKN"
BASE COUNT         5147 a         2892 c         3513 g         5295 t
ORIGIN      
        1 attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg
       61 tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc ctaccatgaa
      121 atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat cggacatttc
      181 tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt
      241 ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg
      301 tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa
      361 gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa aagtaaattt
      421 catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc
      481 tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt
      541 ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat
      601 aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc gattcaattc
      661 ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc
      721 atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg
      781 cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact
      841 gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat tcgctgattg
      901 ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg cagaaacaac
      961 gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg
     1021 tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc
     1081 agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg
     1141 agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc
     1201 tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa
     1261 cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat
     1321 tatgattcaa acaatgaggc tttaaataaa aaaaggaggt tattttttta aggttaagtt
     1381 tcatgatata tgttactact tgctatttca gcttgtgaag aaaatcaaaa actaattttg
     1441 catatcaaaa aaacatgtta caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt
     1501 aaggagatta aaaaatatgg caagaaaaaa gacgattact agagaacaga ttttggcagc
     1561 tgcttatgaa gtagctgcca ccgatggatt tacacgattt actgcgagaa acatcgcgaa
     1621 caaaatgaat tgttccacgc aaccgattta tcttgagttt aaaaatatgg atgacttgag
     1681 acaagctctt tttgagcaga tctatgatca tttacaatat gaagtgttca ctgttgagca
     1741 tacagggaat acgattatcg atcttgcttt taattatatc cattttgcaa gaagagagaa
     1801 gaagttgtat cgttcactgt acttagaaga atatggtgga ggaaaagaga tgcagaattt
     1861 ctcttatact tactttgtga atacagtgaa acgcgatcca gcttatgtga atctttcaga
     1921 agagcagatc gaatctttgt ataatgggac atggatcata gctactggga tcgcagcttt
     1981 gatgtcttca agcatcatcc atccaactga tgaacagatt gaaaagatga tccaagacag
     2041 tatagatgct atcttgaaat tcaaagaacc aatcgaaatc gattgaatat actcaaaagt
     2101 tccacaaatc agtaggtaac aagagatgtg tcaaaataag gaaaagttcg tttttcttac
     2161 agcgtatgca tttttaactc ccgaagaact cttccatgaa tgcaatgaat aaaagagcca
     2221 tacagattga ataaatctgg atggctcttt tattgcaagt atattaaatt tttgcgacta
     2281 gttctttagc gaaagcttct aaattttcga tatcttcttc ttcagcattc agatccactt
     2341 taacattttc ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca
     2401 ccgatttgca gaattcatcg taaaaagtat caccagatcc gcagacaccg taaatttttc
     2461 ccaatagatc caattcttgc aagtcttcat aaaagtctac gatctcatca ggcagatcgc
     2521 catcatcgta tgtataagta gctactacgc aaatatctgc ctcctcgaat tcatctgcat
     2581 ctacttgggt acattcgttg atttcgactt cgatatccag attttcaaat gcttctgcaa
     2641 caatatcggc gatttcttca gtgtttcctg tcatgctagc atatacgatt tttgctaaag
     2701 ccatgatgtg cctcctcgtt tataattatt taacaattat gaattatacc aaaacatcat
     2761 tgaaaagaaa ataaaaccta aagacacctg gggaatgtca tttggctaaa tgtatgccgc
     2821 tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag
     2881 cacgtgaaat tgaaatgatg gcagaatctg gagctttatt agcagatgta cataaaaatc
     2941 tgcgtgattt tataaaacca ggaatcacaa gttgggatat tgaggtattt gtcagaaact
     3001 atatcgagag tcatggcgga attgctgcgc aaattggctt tgaagggtat gaatatgcga
     3061 cttgtatcag tataaatgat gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa
     3121 acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt tctgattctt
     3181 gttgggccta tgtagttgga gaatctacac ctgagatcga ccgattgatg gaagtaacga
     3241 agaaagcttt gtacatcggt attgagcaag cacaagtagg gaatcggatt ggtgacatcg
     3301 gtcatgctat ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt gattttattg
     3361 gtcatggcgt tggtcctacg attcatgaag aaccagcagt tcctcattac ggagaagctg
     3421 gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg gttaataccg
     3481 gaacttggaa aatgaaaatg gatcctaacg gctggactgc gtatacaaga gacggcggac
     3541 tttcttgcca atatgaacat acattggcta ttactaaaga tggtccaaga attttgacat
     3601 cacaaggaga agaaggcact tattgatcta tccagtcaat aggtacggga gaggtacatg
     3661 aaagtttgga acaaaataaa acaaaatgaa aatctgatgc gctttattga gacgactcaa
     3721 aagcgcatca ctgattcaga aatgagtacg acctctgtcg tcgttgctta ttatttactc
     3781 ttgtctttat ttccattgat tattgctttt ggtaatattt taccgtttct ccacatcgat
     3841 caagagactg tactgaccta tttacgagaa gtgattcctg agaccgttta ccagtttatc
     3901 ggtcctgcaa tcaaagacct tttgacacaa agttcaggag gcttgttatc cgtttctgca
     3961 ttagcggctt tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa
     4021 gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc
     4081 attgtcttgt tcatgattgc tattagtggg gtaactatcg ttcttgggtt gggacagctg
     4141 attttggatg ctattcagcc aatcgtacaa atacccgtga actttatcga tcagttccaa
     4201 actttgaagt ggccaatcac attggtagct ttatttgtca tcatgttctt gatttattta
     4261 attgtcccta atgctcagtt gaagttgaag acgatcattc cgggcacgat cttcgctact
     4321 gtcggctgga tgctgttatc acaagtgttt agtatctatg ctaggtattt tgcaacgagg
     4381 atcagcggtt atcagatcat aggaagtttt attgtactca tgctttggct taattttgct
     4441 gctacgatca tcattttggg cggaatcatc aatgcagttg ttcaagaata cgtgacgggt
     4501 catcaaatca aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt gaaaactaaa
     4561 ttttctcata aaaaaaagta gaaacaagag ttcttattgt aaatttatat cagtaattta
     4621 taataattat gactcttaat taaagtaggt gcccatatgc ttgtttcttt tagtatggtt
     4681 ataagatttt ttctgacaat tttattatca ctgatattaa cacctgtatt caaaatcatt
     4741 tcagtacaaa caggtatggt cgacaaacca aatgaaagaa gaataaataa agttccaatg
     4801 ccttcagcgg gcggactgcc tatctttatt tcatttgtta tatcgacttt attcttattc
     4861 cgtaaaatta tccctcaatc gtatattgta ccgatcttga ttgcttcttt agtaatcata
     4921 gtcacaggag tgctcgatga taaatatgag ctgacaccta aacaaaaaag tatagggatc
     4981 ttgattgctt ctcttattat ttattttgta gcagatatac gtattgattc cttcacgctt
     5041 ccttttattg gatatatcca actaggctgg cttagttttc ccgtaacgat tttttggatc
     5101 ttcggtatca cgaatgcggt caatctgatt gacggtttgg atgggctggc ggcagggatt
     5161 tcattgatcg gtttaattac gataggaatc attggatatt ttttcctcca tgcttctaca
     5221 gtgtatattc cagttgttat tttctgtctt gtagcaagta ttatcggttt ttttccttac
     5281 aatttttatc cggcaaagat ttatttaggc gatacaggag ctctattcct agggtttatg
     5341 atggccgtcc tttcactaca agggctgaag aacgtgactt ttgtttcatc gatttctttg
     5401 ctgatcgtca tgggagtgcc ggtaacagac acgttttttg ctatcattag gagaaaagca
     5461 aaccgtgttt ctttttctac agcagataaa aaacatcttc atcaccgact aatcagttta
     5521 ggctttactc ataaaggtgc tgtcctgacg atttatgcga tggctttgat gttttctttc
     5581 acagctatgg tcatgaatta tactggaaga atcggaacga tcatattgat tattgccatg
     5641 ttgtttgctg caattttatt atttgaattg attggtctga tcaatgagaa acatcagttt
     5701 attttaaaaa cattgcgatt tttagggaat aaagagtacc gtacgcaagt aaaacagaaa
     5761 tatttgaaga aatggaaccg tcatatagat gaataacaaa ttaaaccagc cgatccttat
     5821 tagtattgtg acggcaaaca gtgataaaat ttttcggaca ctagataatt ttatttcaac
     5881 tgtaaaagat gaaacagcag taaaaatccg aatttttgac aacaattcac ttcccgagta
     5941 caaaaaaaga ctaacagagt ataggaaata tccctacatc gatatctatt ttcatgatga
     6001 aaattccgga tttggttatg gacataatca caatctgata gctagcgatt ctcgctatgg
     6061 cattatttgt aacccagata ttttagtgga tgaagagaca gttacagcat tggtcttgtt
     6121 attgaaagaa catccggaat gtgcgatgtt agcacctaag attcttaatg aagatggaac
     6181 aactcagcac ctcatccgtg agcagctggc tgtttttgat tacgttttgc gctttgtgcc
     6241 tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat gccgaggatt
     6301 gcctactgat gctaatagtt atgtacggat gatctcaggt agttttatgg tagctgatat
     6361 tgataaattt aaagagataa aggggtttga tgagcgtttc ttcatgtact ttgaagacaa
     6421 cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta ttgtacacac ccttttatgc
     6481 agttatccat ttttacggga agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt
     6541 gacttctatg atgaaatttt tcaataagtg ggggtggcgt tttttctgat gaaaccagaa
     6601 atattagtag tcgttgtgct ttatcagcaa ctattcagtc aatctccagc ttatggacca
     6661 ttaagcaaag cacttagcga aaagcaaatt cagcttatta tttacgataa tagtcctcaa
     6721 aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat
     6781 cctggtttag cggtggctta taattttgcg ttgtctcaag cagatgaagg aacacgttgt
     6841 ctcgtcactc tggatcagga tacgaggcta gtcgacgatt attttgaact attgcgtaaa
     6901 gtaaccttta cagatgaatg tgttgctgca gttccgatga ttttttctgg gagccgacaa
     6961 gtttctcccc tatattcaga tcgttatatc aatcgtacag ctcaagcagt agaagttgaa
     7021 ggcccaacag ctgaacgaat catggcaatc aattcaggtg ttgcctggtc tgtcaaattt
     7081 ctaaaaaaga ttggcgggtt taatcctgct ttttctttag attttttgga tcattggctg
     7141 ttttggaaag tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg aatggaacat
     7201 gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc gataccgttc tattttacaa
     7261 gcagaaaatc gattttatca tgaatatgat caaaggcatt tatccagtca ccgacgacaa
     7321 ttattgttaa gaacagctaa acagttcttg accgtcaaaa accggcatat ctggcggatg
     7381 actttgcgct catatgttaa gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc
     7441 tacctttaat ggaggcagat atttaagaga gcagttagat agtattttat ctcagctttc
     7501 agcagaagat gaagtagtca tttctgatga tgggtcagta gatgatacat gggatatatt
     7561 aactgaatac gcagaaaaag ataagcgaat ccagcttttt tcggggcctc gtcaaggatt
     7621 gatcgctaat tttggttatg cgatccagca gacgaaagga gatctaatct ttttggcaga
     7681 ccaagatgat gtttggttgg agacaaaagt gaaaaagatc actgactatt ttgagcagaa
     7741 tccggatcag ctagtagtag tttctgattt aatcattgtg gacagcgagc tgcaagagat
     7801 ccatgattcg tatttttcgt ttcgaaattc caaagaagga tggcttaata atgtgataag
     7861 aagtcactat attggtgctg gtatggcttt ccggtcttct ttaaaacccg atattttgcc
     7921 gatcccgtca gaagtgccga tgcatgatat gtggattggt ttgctgggag gaaagaagac
     7981 tgggttccta agagaaccgt taactctgta ccgaagacat gatttaaacg cgagtgagat
     8041 tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt
     8101 gatcaaacga aaatggttta aacgttgata aaagatgttt aaatccttaa tatttccatg
     8161 acaaatataa caattgatga gataataaca aagtgaaatt tgaaatcaaa aaggagagca
     8221 gatatccatg aagggaatca ttcttgctgg gggcagcgga acacgtctgt accctttaac
     8281 aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt attatccaat
     8341 gtctactctg atgttggcgg gaatcaaaga aattctgatt atttcaacgc cacaagatac
     8401 accacgtttc aaggaattat ttggaaacgg aaatgattta ggactgcaga tcgaatatgc
     8461 agtacaagaa agtccagatg gattggcaca agcgtttatc attggagaag aatttattgg
     8521 agatgatagt gtctgcttgg tattagggga caatatttac tatggtggcg gtctgtctaa
     8581 aatgttgcaa cgagcagctt caaaagatac aggcgcgaca gtatttggct atcatgtcaa
     8641 tgatccagaa cgctttggtg tagtagaatt tgatgagaat atgcaagctt tgtcgattga
     8701 agaaaagcct gctcagccaa aatcaaatta cgctgtaact ggcctttatt tttatgacaa
     8761 tgaggtaatt tctatcgcta aaggtatcaa accttcagaa cgtggagaac tagagattac
     8821 ggatgtcaat aaagcatatt tggataaagg aaaactatcc gtagaactta tgggacgcgg
     8881 atttgcttgg cttgatacag gaacacatga atcattgctt gaagcatcta cctttatcga
     8941 aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg cttaccgtat
     9001 gggctacatc gataaggaac aattgctggc ccttgcacag ccattgaaaa aaaatcaata
     9061 cggtcaatat ttattgaatt tggccgctga ataaggagtc cttacagatg aaagtaacag
     9121 atacaaaatt aacagatgtc aaaatcgtag aaatggacgt ctttggcgat catcgcggtt
     9181 tcttcacaga aagttattca aaagcaaaat ttgcagaaca tggattgaaa tatgactttg
     9241 ttcaagacaa ccattcacta tcagctgaag ctggtgtcct tcgggggctt catttccaaa
     9301 aaggagaagc tgcacagaca aaattgattc gggtagtcac tggagcagtt ttagatgtag
     9361 tagtggatat ccgcaaaggt agcccgactt atggtcaatg ggacggattt atcttatctg
     9421 aacataatca tcgacagttg cttgtaccta aaggatatgc ccatggattc gttactttga
     9481 caccaaatgt gaacttcctt tataagtgtg ataattacta taatgcagaa gcagatgggg
     9541 gcatcgcatt tgatgatcca gatttagcga ttgattggcc aattgatcca tcaagagcga
     9601 ttatgtcgga aaaagatcac cgtcatccga cattaaaaga atttgaagca gaaaatccat
     9661 ttgtttacgg agaaatctaa gaattaagga gaaattttct tatgaaaaac atcattgtta
     9721 ccggaggtgc cggattcatc ggctctaatt tcgttcatta cgtggtaaac aatcatccag
     9781 aagtgcatgt cactgttttg gataaactga cttatgcagg aaacaaagag aacttggctg
     9841 gacttcctag cgatcgcgta gaattggtcg taggagatgt tgcagatgca gaattagtgg
     9901 accgtctagt aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc cataacgata
     9961 attcattaaa agatcctttc ccatttgtac aaacgaatat cattggtacg tatacacttt
    10021 tagaagcttg ccgcaagtat gatgtacgct atcatcacgt atctactgac gaggtttacg
    10081 gcgatcttcc attacgtgaa gacctgccag gacatgggga aggcgaagga gaaaaattca
    10141 ctgcggaaac accatataat ccttcaagtc catattcttc tacaaaagct ggttcagatc
    10201 tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc aaccatttca aactgttcaa
    10261 acaactatgg tccttatcag catattgaaa aatttattcc tcgccaagta acgaatatct
    10321 taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac tggatccata
    10381 cgaatgatca ttcttcagca gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct
    10441 atctgatcgg tgcagacggt gaagaagata ataagaccgt gatggaattg atcttagaaa
    10501 tgatgggtca accaaaagat gcgtacgacc atgtaaacga ccgtgcagga cacgacttgc
    10561 gttatgcaat cgattcgaca aaattacggg aagagttagg atggaagcct gagtttacga
    10621 acttccgtga tgggttggct gacacgatca aatggtacga agaacatgaa gactggtgga
    10681 aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa tggacaataa tacagagaaa
    10741 atgtcactgt attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa
    10801 aataaaaatc aaggagcgga taatcatatg tttttattaa ctggaggaaa tgggcaacta
    10861 ggtacagaat tgcgccattt attagatgaa aaaaatctgc aatatgtttc aacagatgca
    10921 aaagacttgg atatcacaga tgccgagaaa acaatggctt acatcacaga attaaaacct
    10981 gatgttattt tccattgtgc agcttacaca gcagtagata aagcagaaga agaagcaaaa
    11041 gaattagatg agaagatcaa tgttgatggt acaagaaatg tagcacttgc agcaaaagct
    11101 gtgaatgctc aattcgtcta tatcagtaca gactatgttt tcgatggaaa aaacaaaaat
    11161 gtagaatacg aagtagacga ccaaacgaat ccactaaatg aatatggtcg tacgaaatta
    11221 ttaggcgaaa aagcagtaga agagatttta gaagactatt atatcatacg aacttcttgg
    11281 gttttcggga tctatggaca caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc
    11341 gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg tactttggca
    11401 gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac
    11461 gaaaatagct gttcttggta tgaattcgca aaagaaatat tgaaagatac agatgtagaa
    11521 gttgcacctg ttacgtcaga agaatttccg caaaaagcaa cacgtccgca gtattcagtc
    11581 atgagcctga aaaagacaga agagttagga tttgtcatac caacttggca agaagcactt
    11641 gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat ttccttttat caaatagaat
    11701 ttggagcatg atatggaaaa acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg
    11761 aaccaggcag attcgaattt ggtgcttgac ctgtttctaa aactccgcta tcttattggc
    11821 gttttagtca tcgtggtcat cgttactttt aatttaaacg gaagttcact tggtgtttgg
    11881 gataaatatg tgtcgcaaag agacgatggg aaaaaaactg atgtgatttt tggacagaat
    11941 cgggaagtgc ggtcagacga atggctggtg cagacacctt tttacctctc acaagctgaa
    12001 gaggactttc cgttggtgaa cgaggattat tcattaaacg gacaaaacat gatcattgcg
    12061 tataattctc ctgtaaagga tattacggtt atcggaaaac cattcaactg gggattcttt
    12121 ttcctcggaa gagatcgagg actttccttt tattgggcga tgaaattagt cgtcatggtc
    12181 ttgcttggat tcgaagtact gatgatatta acgaagagaa aaaaagctct atcgctaatc
    12241 ggtgcatttg cgctgacatt ttctccagca gtccagtggt ggttcatgca acatgtgggc
    12301 gatttgattt tctttacttt gggactgatg actgcttttt atcattattt ctacaatcat
    12361 gataaaaaat ggctacgaac gttgatgatg cttttggtgg ttattttcgg tattgggttt
    12421 attttagtga tttacccagc ccatcaagtg atgctggcgt acttgctgat tttttatttt
    12481 gttggcctgc ttatttacta tagcaagaaa gtttcatggg atctgttgga tgtagtgttg
    12541 attggagggg cagttttatt tattggcgga gtcatggttc atttctggct aacgtcaaaa
    12601 gacgcattgt tagcttcttt gaatacctta tatccaggaa aacgggtaag tactggagga
    12661 aagtggacga ttggcgaatt tttttctttc cttacaaatt ggaaaattcc ttttgaagat
    12721 atcaactatt cgaataactc tgaagtggcc ctgttctatc acttttttcc aactgtattc
    12781 ctagcaagtc catttgtgtt atttggcaaa aaagatagcg aacaaaagtt actgggacgt
    12841 attttgatga ttttttgtct gttttctatc ttttggatta ccgttgggtt gccaaaatgg
    12901 ctagcagaaa tcactctgct gtcttatgtt cctccattaa gagccatcct tacttttagt
    12961 tttgcggctt gtttattatc gatctggttt atcgcttata tttggagaga agatgttatt
    13021 cctatttggt ataaattcat tttgttggca gtaaatgcat ggtcttattt ctatgcgatt
    13081 acgcattcga aaatggataa ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg
    13141 ggaacagtga ttttagcttt tgcattattt ggctggagac gcttgtttta cctcgctttt
    13201 gccgccttag ttatcgtatc tggctttttc gtcaatccag tcgtagaagg tacaggagcg
    13261 atttttgaaa aaacactggc aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa
    13321 gtttggttgt ctgaagatga attatataac ttcaccccaa cattgggagt gaaatccttc
    13381 aatacagtac gcttttatcc agatatagct gcatggaaga aaatcgatcc gaaagaagaa
    13441 tacgagaagt attataatcg gtatgctcat acaagagctt ttatcgctaa ggaagataca
    13501 aagttcattt tagatcgtcc agatatgttt tcggtcacgt tgaattttga agacgcagaa
    13561 aaattaggga tcgactatgt gatcagtaaa cgaccattaa ctgattacaa ccaattatat
    13621 gaagcgcagt tcacgcagct atatggaccg gataaagacg gttatatgat ttttaaagta
    13681 gaatatccgg attatccaga tgtaccacaa acacaaactc aggaaaatca gctagtcgta
    13741 cagtaatagg ctagctgaga ataatcctga aatgttagta gaaaggagag tggagataat
    13801 tcaagcaatc gtacaaaaat ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttact
    13861 gacgctagtc ttttttgttt ggacagcagg gttaggtttt ccagcaactt tgtttatata
    13921 taacaatagt tcattactta tcgcttttag tgtggctatt ttgctcattt taaatgttta
    13981 taaagcaaaa ggcgtcgatt ttctttatct aggtgttgcg cttgcccttt tcattttctt
    14041 ttcttacttt cgggaattaa gaaatgagtc tttatttgga gacttactgt ttcctttgat
    14101 tattttattt tgtttagtaa ttaaatggat cgagtggaca aaatgggatc gcggtattta
    14161 tctgattgtc ttttcggtct tcctcggaat cacgttatat cgtgtattta cagagatcca
    14221 agtaccagaa ggacaaagca tctgggctcc aggcaattcg ctttctgata tttggatcaa
    14281 tacgaataca atcggaagtt ctttgatgac tcttgcttta ttgatcagtg gatttgccag
    14341 ttcttttgaa cgatggtatg tacgaatcat cggtgcagta gcagttgttg cagcttttct
    14401 cgggatttgg gtctgccaat ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt
    14461 ggatctttta cctaaatcgt tgatgcgtgt cattcgtgcg ccatttattg cttacacggt
    14521 tacgattctt ttagctttgc cgatttctta tctggcggct gtctctgaaa aagtcaattt
    14581 gtttaccggt cgagaagata tttggcataa attctatcag acgctaggag aaaaatctga
    14641 acaaatttta ctaggaatga aaacatttat cttccaacga ggaaaccaca atagttataa
    14701 ttctattttg aatatctatg gattgatagg atttgggatt gctgctttgc tgcttatcct
    14761 ttttattggt cgcttaacgt tgaaagcaga tttatccaac gggcagatga cttttatctg
    14821 ggcctttttt gcggtgatga tgcagtcatt tatggaggat acattgactt catacccatg
    14881 ggttccgatt atttatatcc tgttggctat ggccgttcat cgatatgatg aaccagaagt
    14941 tgcggaggaa ttctatgtta tggaagaggc atatgacgat atgacttatg atgatacatc
    15001 ttatgaggaa caaccagata gcgctcattc ttccaggatc caacgctatc attgataggc
    15061 acaaaatacg agagaggctg tgacaaccgt cgcagccttt tttggtttat tgcaattgtc
    15121 tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg tatcatagaa aattaagaaa
    15181 gaaataagaa cgcagctcgg taccaaaaat atggtaaaat ggcacttgac tgtaattcac
    15241 tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt atttatttat gaaggacatt
    15301 tatcagaaca gaaaattatt gctacagttt tcgatcaatg acttcaaatc aagatatgca
    15361 ggatcgtttt taggaatctt atgggcgttt atcaatccgc tatttactgt attgatttat
    15421 tggctggtat ttggtgttgg actgaaagca caaatgatcg atgcaaaata cccatttatc
    15481 gtatatttag ttaccgggat gattccctgg ttcttcttct cagatgcatt tgcatcaact
    15541 acggttgttt ttcgggaata tacttattta gtaaagaaag tcgtattcaa tattcgtatc
    15601 ttgcccacga cgaaaatcct atccaatctg tacacccatt tattcttcat tctgattggc
    15661 atagctgtcg cgcttataaa tggtatatat ccttcagcaa tgtctttaca gctgatttat
    15721 tatttatttt gcatgtgtgc tttcttgaca ggattgactt ggctgacggc atcgatccaa
    15781 ccatttttac ctgatatcat gcaattaatt acgatcgtgc tgcagttgat catgtggaca
    15841 ttaccgatat tatggagccc taatcagttt gattcaggtc ttgtaagcat attgaaatta
    15901 aatccgctgt actatgttgt tcaaggttat cgtgagtcat ttttaagtga agcttggttc
    15961 tgggagcatg gaacatatac tttgtatttc tgggttttca cgttagtaat gttcgtaatc
    16021 ggctctgcag tgttccgtag attgaaacca catttttcag atgtgctgta aggagaatag
    16081 ctatgacaga atatgcaatt agactaaaag atatcacaaa atcttataat atgtacgcaa
    16141 aaccttcgga tcgatttcgt gaagccctca atccttttaa gaaatcctat catgatgtat
    16201 tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg tgagatgatc ggtttcgtgg
    16261 gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat tacgggagta ctgacaccaa
    16321 catccggcac gatggaaatc gaaggaaaaa tctcagcttt gctagaacta ggatcaggat
    16381 tcaatcctga gtattctggc tatgaaaata tctatttaaa tggaatggtt ctaggatttt
    16441 caagagaaga agtcgatgaa atggtcgatg atatcattga atttgctgac atcggagacc
    16501 atatttatca acctgtaaaa acttattcaa gcgggatgtt cgttcgtcta gcttttgctg
    16561 tagcaatcaa tgtgaatccc gatattttga ttgtggatga agcattggca gtaggcgatc
    16621 ttgaatttca actaaaatgt atggaaaaat ttacagaaat caagaactcg ggaaagacga
    16681 ttttattcgt ttctcatgat atcagttcga ttcgtcgttt ttgtgatcgt tcctattggt
    16741 tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga agtaacgacg aattatgaaa
    16801 actttttaaa gaaaaaatca gttaaaacag tggatcggaa taaaaac
//
LOCUS       DACCSK010000058        16627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCSK010000058 DACCSK010000000
VERSION     DACCSK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.059"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..832)
                     /locus_tag="IVP39_001656"
     CDS             complement(<1..832)
                     /locus_tag="IVP39_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7586731.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1644)
                     /locus_tag="IVP39_001657"
                     /pseudo
     CDS             complement(846..1644)
                     /locus_tag="IVP39_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
     gene            complement(1677..1961)
                     /locus_tag="IVP39_001658"
     CDS             complement(1677..1961)
                     /locus_tag="IVP39_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7586732.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1958..2959)
                     /locus_tag="IVP39_001659"
     CDS             complement(1958..2959)
                     /locus_tag="IVP39_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7586733.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2961..3863)
                     /gene="gnd"
                     /locus_tag="IVP39_001660"
     CDS             complement(2961..3863)
                     /gene="gnd"
                     /locus_tag="IVP39_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7586734.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            4027..4875
                     /locus_tag="IVP39_001661"
     CDS             4027..4875
                     /locus_tag="IVP39_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7586735.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5521..5727
                     /locus_tag="IVP39_001662"
     CDS             5521..5727
                     /locus_tag="IVP39_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7586736.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5929..6930)
                     /locus_tag="IVP39_001663"
     CDS             complement(5929..6930)
                     /locus_tag="IVP39_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7586737.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6935..8848)
                     /locus_tag="IVP39_001664"
     CDS             complement(6935..8848)
                     /locus_tag="IVP39_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7586738.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(9016..9522)
                     /locus_tag="IVP39_001665"
     CDS             complement(9016..9522)
                     /locus_tag="IVP39_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7586739.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYFIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9682..10122
                     /locus_tag="IVP39_001666"
     CDS             9682..10122
                     /locus_tag="IVP39_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7586740.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(10148..11305)
                     /locus_tag="IVP39_001667"
     CDS             complement(10148..11305)
                     /locus_tag="IVP39_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586741.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(11308..11664)
                     /locus_tag="IVP39_001668"
     CDS             complement(11308..11664)
                     /locus_tag="IVP39_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586742.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(11962..12855)
                     /gene="sgrA"
                     /locus_tag="IVP39_001669"
     CDS             complement(11962..12855)
                     /gene="sgrA"
                     /locus_tag="IVP39_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7586743.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            complement(13051..13890)
                     /locus_tag="IVP39_001670"
     CDS             complement(13051..13890)
                     /locus_tag="IVP39_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7586744.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            14075..14962
                     /locus_tag="IVP39_001671"
     CDS             14075..14962
                     /locus_tag="IVP39_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7586745.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(15546..16241)
                     /locus_tag="IVP39_001672"
     CDS             complement(15546..16241)
                     /locus_tag="IVP39_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7586746.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGAYYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(16225..16623)
                     /locus_tag="IVP39_001673"
     CDS             complement(16225..16623)
                     /locus_tag="IVP39_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7586747.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         4983 a         3376 c         2561 g         5707 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgccg catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat cctgttataa aaaaagcagc
     1501 ttgtctgtaa aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc
     1561 aagaggtgtc catggagcac aattactagc taaagtaggg atgactccga aatcgattct
     1621 tgcaagattg gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac
     1681 gaaatcgatt tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat
     1741 tctccagaat aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta
     1801 ggtagcgcct tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat
     1861 gatttcaaga gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg
     1921 cctgttttca cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct
     1981 ttgtctggac agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac
     2041 atttttcacc cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca
     2101 ctacttgttc aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat
     2161 ctgtggcata tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa
     2221 ctagagcgcc tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt
     2281 tatcagaaat catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg
     2341 attgttccag taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat
     2401 ctgatttgta aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga
     2461 ttactccgct tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc
     2521 taccgacgaa ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg
     2581 tcgtgcgtcg aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt
     2641 caactaaagc aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa
     2701 tattgttgta accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa
     2761 aatctcgtcg aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact
     2821 ccgctagttc ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg
     2881 actttaatct ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg
     2941 ctatatgata atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca
     3001 tttcttagtg ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc
     3061 atgaacaaac tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc
     3121 gtccattttg cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt
     3181 tcgtttaact gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc
     3241 caaacacgtg cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat
     3301 ccttcaccga ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag
     3361 tgtccgcttc cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat
     3421 aattgttcca cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt
     3481 cgtgctcctt cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga
     3541 aactcttctg ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg
     3601 tctcctggtc cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca
     3661 ggaagcatta accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct
     3721 tgataagcag gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt
     3781 ccttgtactt tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc
     3841 aaaccaatta caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa
     3901 aaaatttttt tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc
     3961 cttgtatctt taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga
     4021 attctcatga gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca
     4081 aaagaacagg ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct
     4141 attagcaatt tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa
     4201 aaacttggtt ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat
     4261 ctatctgcta tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg
     4321 caaaaggtgt tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa
     4381 aaggatctaa aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt
     4441 gttggcggtt cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc
     4501 gcaacttctc acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa
     4561 aaagactgtg ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct
     4621 gaaaccgtaa gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc
     4681 cttgctaaac ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct
     4741 gaagctttag cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg
     4801 atgttttaca acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct
     4861 gaaatcaaag aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca
     4921 ctaccaaata tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag
     4981 tcggtgggtt agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga
     5041 gttacttgtt agatgcgata acaaagttag tatacagtag taaaaaattt atcttttctt
     5101 ttttttatta tttttgtttc ttattttcta aaaaattaaa aaaaattttg gtgaattaag
     5161 tatttccttg ataacactgg cttttggcct cattttaggt tatcatttgt aattttattt
     5221 cacagttgat atgattgtta tgtaaaataa aaaagtttag gatacatcga atttttgggg
     5281 aaattctttt gggggtatcc taaatctttt ctattatatt cccatatttt ctattattgg
     5341 tctagacgta tgatggtaaa ctttgtacct atctgattgc tgaatcatta attgcttatc
     5401 attagccatc tttgtttcaa gttcgctaga tgatcatttt attcacatcc taacaaactt
     5461 gtcacattta tagattctta atctatttat catacaatta taaaaaagga ggtgctgctt
     5521 atgaaagcta tccatatttc tttgcttttt ttcgctagtg cattgatttt atttgctatt
     5581 catctttgtt cccagcctta tatcaataac aacggaatat tagtggaacc agcattcttt
     5641 tgcttaccac tcggcttttt gtcccttctt gcctccgcag gcttcggatt tgttgctttt
     5701 tttaaacgat caaaacaaca aatctgactt ataaaattta cgtaaaagag atcgtgacaa
     5761 taatcgtgtg atattcccaa atttcgaata aaaggtgccc cttgccatct ctaactcctc
     5821 agtcacaagt tacaatattc ataaaccatg aaaagcttgt ttattgaaat tgctccttgt
     5881 gacttggagc taagtgacag attacaacct cttttgcatc ataagacttt actccttgaa
     5941 aaacattact aacttttgat atgtttcttg cgcttgttta ttgctgaaat caaactgata
     6001 ttcatggttc aaaacagcat ttttctccgc ataatctact tttgtgactt tcacccccgc
     6061 tttctcaaat acagaagtta cttcttccat ttgtttcgta aacgtatttg tatttccatc
     6121 cgttaagaat gtgttttgcg tccaacgatc cgcatgtctg accaaggcta aacttttgac
     6181 cttctctgat tgttcaaagt ccttattatc aaagtaagcc catccacatt tttcataaag
     6241 aaagctagag attttatcac ttacttgagt aagtgcagaa ggatccaaca gaccagacaa
     6301 agaaataaac ttcgtaatct gcttgttctc caaagtcgga tcaagatgat cagctttttt
     6361 aatttcttca ttttgaagtg ctaacaaata ttctccacta atttgtgcac ctgccgaatt
     6421 tccaccaatc gaaatattcg accaatctgc ttgatctttt atctcttttt tagctgcttg
     6481 aatggcttcg ttcaattgga taactggtgt aggatgtttt gcttccggtg atcgttcata
     6541 atctatattt acgatgattt gctgcgtgtc tgaacataga tattccaaat aatcacgaca
     6601 atcattttta tcccctccta catatgcgcc tccatgcatc cagaaaagaa tcggtaattt
     6661 tttcttttcc actttcggtg tgtagatatc catattatta tttttgtact ttgaaggata
     6721 ggatatttct ttttttatat cgatttgttc gattttttgc tggaaatcat ctggtcgggc
     6781 atatacttgt tctttttcac ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt
     6841 tggtgtgatt tgattaatca aaaatacagt gactaaaata actacgatga taactggtac
     6901 tgcaatcact attcgcttga accatttcat tcatttaccc tctctttctt cgccaaatcc
     6961 atgtgataat cgtgctaaca gataaaaatc cacctgaata aatgagaagc gaatgtttct
     7021 tttctccagt tttcggcaac attctttttt ttgcatctgt ctttttttgt actttcactg
     7081 tcatctgaat cggttgcgta tcatcacttg attgatcctc tcgaggttca ttctttccct
     7141 tattgtctgc attctctgtc ggatcttgct tttttggtgg agctactggg atttttttgt
     7201 aatagaaaat aatagttggt atcgtctcat taaaaatccc ttcagtcggt ccttcaacat
     7261 ttgctaattc gtatccttcg aatgtctttt gctctgtcat ataattttct ccataagctc
     7321 cgtaaagctg gatttcgtcc tggtctttga gtagactttt attgttttca tccacatact
     7381 ttacgtttat ctgatcatgg aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg
     7441 cagcatggtc aacttcctgt gcctttccat gccctgctcc ttctactaat aaaagatctt
     7501 tataccctcc tgataactca tataattttt gcgcgttaga cacaggtact acatcatctg
     7561 ctgttccatg gataatcaat ttaggcaatg aatctgacat tccaatcgta ggtagagcag
     7621 cttttgtatc caaatgcatc tcctgatcaa aatagtattg tttcaaaagg cccataaata
     7681 aatcttcatg ctcttgatcc ccaatgatgt ctaactgatt ttttatggca tccggtagtg
     7741 cgggggctac tgtctggctg tacgtaaaac gtagttgttc atcaattgat gcaaatccgc
     7801 aattttcaat aattcctttg acattgacag gaatatcttt cgctaataca gacatcatcg
     7861 tagctgctcc catcgaacca ccataaagta aaatcttttg agatggccgc tctcgaactt
     7921 cttgatttat ccagtaaagg acgtcatctg actcatattg tccataggtc acataatcgc
     7981 ctccgctatt accagtcgct ctggaatcaa caaataataa attatacccg gccttataaa
     8041 aatcattgta ttcttctgta acgatcccgt tattccaatt ccctcgaaat ccgccatgga
     8101 tcatgactgt tttatctgaa ccttgatcga cataatatgc atatacatcc atcattttgc
     8161 catcttgttt gtttagtaca gtttgatgaa gttgttttgt atttggatag aagcctgtgt
     8221 tttctgtagg cgttgtccca tcatctgtac ctccaagcat cgacatcaac acactgtttt
     8281 ttgcaaaagc gatatcgtac aatagataag tagcaatcag atgtttgata atctctttat
     8341 ctacaccata atcattaaca gcaagctcag tgactaataa ctctattaat tttgacttga
     8401 tgtcttctgt tggtccatta agtatttctg tcatctgttt accgaagtaa ttcaaatatt
     8461 ctttaccatt gaaattttca cctttaccga tatcatcatt ttctgtccca ttctctttgt
     8521 cacttacttt agcagaagag gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg
     8581 tttgttggtt atctgtggta gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa
     8641 ccgttgtttc agtatttgtt tctgtttgtg aagtactcga tttcgtttca tttccagcat
     8701 cagtagtttc tgtggtttct acctcccgag attccgattc ggaagaaatg ctctctccat
     8761 atactgtcca ttgcgggacg cttgtactta cagcaaataa gatgaccaac gctcccttta
     8821 atacttgttt tgttctcaag ctattcactc ctttttatgt ttctaggaga attatatgta
     8881 aacgcaaaca ttttttatac aaaaaataaa tggtttatcc cataaaaata gaacaggatt
     8941 attttctcat tttacgtaat aaagcagaat ggataaacca gtatccattc tgcttttttt
     9001 aaacgaagaa acaacttatt gctccaaaaa tgtcagaaat cgtttatccc aaattttctc
     9061 gtaaaaagcg attgtttcct ttgctttcat aaaaggatta tcataagttg tcgttgctcc
     9121 tctacacatc ataagctggt atcctaatcc atgagcaaac ttcacagtcg tatctataaa
     9181 atactctgct tgtgccccac ataattcgat cgaatgacag tccaattcag ttaataaaac
     9241 actcaaatta gtcttataaa atgaattcgc atgggttttt ctcacaaaat aactgtgctc
     9301 aggaatagat aattccgaca atagctccca tggttttgtt tgtggcacta attgagcatc
     9361 ttcgtgttga acgaaaataa ctggtttccc aatcgtttcg tatgttttga ttcttttatt
     9421 cactaatgct gtcactgttt ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt
     9481 ttgtaaatcg atcactacta ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt
     9541 ttttcttgat ttaatagtat ttcttatcta gctagttcca ttatacgtga caacattttc
     9601 ttattttaca cctctaaatg gcacttttcg actatttctt catgttatta tttcataaat
     9661 acttagctat tggaggaatg tatgatgatt aaattgtata caggttctgg ctgctcgtct
     9721 tcaagacgcg caaaacgttg gcttatcgtc catgatatac cgtttatcga acagaactta
     9781 attcgtcaac cactaaccaa aaatgaattt atccacttac taaaattgac aaacaacggc
     9841 gtgaccgaaa tgctctctac tcattctatt agttataaaa atcttgttca cagaatagat
     9901 gaactcagtt tgagtgaact atatattgag attcgtacat ccccttcttt attgaaaaaa
     9961 ccgattatct acgaccaatc tgcaaaactt ttgactggat ttagcgaaga taaaatcagg
    10021 atgttccttt ctaaaactaa aagaaagcaa gagctcaacg aactcttgct ttctggtcat
    10081 cttgtcgatt ttaaagaaaa taatcttttt gcaatgtctt aattttggca taaacaatct
    10141 gcttttctta attaacgaaa tccaattttt ccaatatgga tctatttttc tcattcctga
    10201 tttttatgat ttcagctttc agacgcgccc aatcattttt tgtaggttgt ccattccagt
    10261 aaaatatctg tgtattcgtt tgatcatatg cctttttgtc gataggataa tcgctaatga
    10321 caatatcagg taactgatca gcggatgcac tatattttat tgggcgttca gtcatagata
    10381 atatcctttt ctttgcccat aattcctgtg ttttaccata ctttgattga atatagaata
    10441 tgacaggttg ttcgtgtttt ataaaaatat ccctgactaa catacagtat tgaaaaatca
    10501 accgtctgtt cttttcataa taagtattaa gctctggaat tctgcgagct accccactaa
    10561 aaaactgttg catgattgga tacatcactg gaaatatatg ctgaaaatct ctgtcttctg
    10621 ctgattttcc aaaagcatcc accttctttt ttgtcccaaa aacccttgaa tacaaatgga
    10681 tcattgccaa attgacatat agataatttt tttctgaagt tgtaaaacaa atcttgaagt
    10741 ttttttcaaa attttctaca atagacgatt caatccagcc ccaactttct tgtactttat
    10801 ctggagtatc caacaaatct tcttccaaat aggtgttcat tacaccaaac ataaaataaa
    10861 ggtaagcttg ctctgttgga gtgatgtgat tcatcgacca gaagtttgct tcaaaaaaag
    10921 gctctagtat gtaatgcgta aactcttctt ttgttatcaa tggatgcaca aatttgttca
    10981 tctcttctgg gacatacgta atcgagtggc cttgtttcat tcgttgcaac gaaattctta
    11041 gtaggctgat ccatttcttt ctatctatac tacgcgtata tggcgcatgt ttcgcaaatt
    11101 tttctaatag ttgacgttta ttttttatac ttgataatat atcagaatga ttttcgtcga
    11161 atgactgcca atacatcaag tgaaaaaagt atcggatacg gtattcttct ccttctatcg
    11221 gattaccttt tttcaagctg caagataaat gatagttttt caaaatagta ttaagttctc
    11281 ttaattttct ttgaatcgta cgcaaactta tataattttt tgactaagat tttctaagga
    11341 ttgataagta cctttgtaaa gtgaatccag taaaatataa ccaatcgttc cctctaaaag
    11401 tatagaataa actttactca gtaaaaactg agaagaaaac tcgactttta tcatcttgtt
    11461 ttctgaaaga aaagataatt tatattctgg atacctttcg caaaacgatt ctgcttgttg
    11521 aatcaccgaa ttcatcttag ccagggtgta gtttagatct aaagcgatat ccttcaccac
    11581 atataatttc tcgtctcttt cataacaata gttcaaaaat tgatatactt gcctttcatt
    11641 gatatcaata aattcttcaa acaaagtaca tactcctttt tatctattat ttagacttat
    11701 tccgacagct acatgaataa cgtacaaata gattcgttac tgatttttca gagatactac
    11761 catgcgcagt gtgcattgtc aattattctt gctcttattt tccatttgtg ttcctgttat
    11821 ttcctgtttc tcaaatatgt tagtaaacaa aaatagatct ggcaaagcta caacttcgcc
    11881 aggcaataca gcactctcga atgaaatatt cactcaaaaa tctatcgttt tattcaattt
    11941 tagatctgtt gcttttattc tttatttcac tcgatggaag agagaacgaa aatagcttgc
    12001 tcctacaatc aaaatgagta acacacccat ccatgtaatt agcttgttca aggttctact
    12061 accagtttct ggtaaatcgc tttttctatt atctgtttct ttcgcttttt cttgatgatc
    12121 aattccacga gaaacaaaat caggctgttt tagcacactt tttaggttgc tccctgcatt
    12181 tctcttttgt tccttagttg gtatgatagg taccaaatca agttcattat ttacttttcc
    12241 tgtgctgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac tctccgtact
    12301 gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg tactgctttc
    12361 accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc ttggcggtcc
    12421 aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc catcataaaa
    12481 aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt ctgcagaaat
    12541 cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa cttccccaat
    12601 aactttttct gaacttccat taccatccca accatatgtt agaacagcat gtacatcact
    12661 agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg tcatgtcata
    12721 aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt catttgcaga
    12781 agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa ataatacggt
    12841 cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga taaaggaatt
    12901 atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc
    12961 gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa
    13021 gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat
    13081 ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt
    13141 agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg
    13201 gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc
    13261 tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact
    13321 gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt
    13381 catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat
    13441 taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga
    13501 tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc
    13561 tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc
    13621 aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc
    13681 ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac
    13741 agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat
    13801 atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga ctattaaaac
    13861 agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac
    13921 aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata
    13981 gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta
    14041 attcttctgt tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact
    14101 actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa
    14161 tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc
    14221 aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga
    14281 tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat
    14341 caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac
    14401 atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa
    14461 attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca
    14521 gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg
    14581 ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga
    14641 tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc
    14701 atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta
    14761 tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaaga
    14821 caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg atacggaatt
    14881 catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatta aagatcctta
    14941 tgtaaaaaat atattcaaat aaacaaattt gacccaattg gggatgtggc aaaatttgag
    15001 aagtgatttt tggaaattcc ttcttatttt tgaggcaaac cacttttgcc acaacttcat
    15061 cggggccttt ttttatttgt accaaaacaa atcactggat ttgttacaat catgaggata
    15121 taatttaaaa aagtttttta tttctatcac atgaatcact gcgccattaa gtagattctt
    15181 tcatgcttcc ctattttttc acttgccgta tcgcttcaaa aacaactatc ataaactcaa
    15241 actcgactga aaagttattt taaaaaagac taaaaatatc ccttctggac ttgttcttct
    15301 tattctgtta tactagagaa acacgaaaga tatttttatg cgacactttt catttgtttt
    15361 tatgaaattt attcaaaact ggcgtaactt cagagttaca aggatgtatt agaggttagg
    15421 gaatacatcg tttaaatgag aaataagtaa tagtcaccca aactttaaat taatgaatat
    15481 tgaatcgtca gatagcctcc tcgagttttc gagaaggcta tcgtttattt tatggagaaa
    15541 ttgattcaga agtaatcagc gacctttttt agttcaccaa tcgtttctgc ttggaaagtt
    15601 tgcacccaat cttcttggaa gcccctcact gctttatcaa tcgagggcat ctctaatact
    15661 tgatctagta gctctttacc taacgttatc gcatgagctc cagatcgaat ggctgtagtg
    15721 acttgttcac aatttttgaa actagctgct aatatttttg ttggtctttg cgtacgttcg
    15781 atctcagtcc gcaacgaatc aatcactttc atagcatcaa tgccttgatt ttgcatcctg
    15841 ttataataag gtgcaatata atatgctcca ttattaatag ctaaataccc ttgtaattct
    15901 gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt tcaacttttt gatcgcttga
    15961 aggcctgctt gattcaccgg caccttgata aacgtatctt ttcccagttc ttctatgata
    16021 actcgagcat cctctatcat ttcttcttcc gttcttccta ttgtctgaat atggatagga
    16081 atctgtccga ttattttttt gatttctttc atatgcgaga agaagtttga cgctccttct
    16141 tttttcagaa ttgtaggatt tgttgttacg cctgttaaat tcaaaaactg ttgacaagaa
    16201 taaatacttt ttgtatttgc tgtatcaagc atgatttcca tatagctttc ccccattctg
    16261 atcctcttga tcgattttaa tgattgtccc ctttcttaaa tgcggcattt ccttatcttc
    16321 tacatacatt gttcctggaa gttccggctc atgagatcca tcaaatttta tcgtgatatg
    16381 tcctaattca ttcaaattag tttggacaac atctcctact gctgtgatta catacatttg
    16441 tttatcaaaa cagagcttca tatcacaagt tatttctttt cttacctgat tcaattcaat
    16501 cttataacaa taatctgtta atgtttccgg tgcttcatct ccaaataaaa tcattatttt
    16561 ttctttcctg aatacttctg cttctatacc aacatctgtt acaaccgttt caaagatcgt
    16621 catctct
//
LOCUS       DACCSK010000059        16197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCSK010000059 DACCSK010000000
VERSION     DACCSK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.060"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IVP39_001674"
     CDS             complement(20..904)
                     /gene="rsmA"
                     /locus_tag="IVP39_001674"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7586748.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IVP39_001675"
     CDS             complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="IVP39_001675"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7586749.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1459..2235)
                     /locus_tag="IVP39_001676"
     CDS             complement(1459..2235)
                     /locus_tag="IVP39_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7586750.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2390..3712)
                     /locus_tag="IVP39_001677"
     CDS             complement(2390..3712)
                     /locus_tag="IVP39_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7586751.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3887..4474
                     /locus_tag="IVP39_001678"
     CDS             3887..4474
                     /locus_tag="IVP39_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586752.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IVP39_001679"
     CDS             complement(4953..6962)
                     /gene="metG"
                     /locus_tag="IVP39_001679"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7586753.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7230..7508
                     /locus_tag="IVP39_001680"
     CDS             7230..7508
                     /locus_tag="IVP39_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586754.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7605..8426
                     /locus_tag="IVP39_001681"
     CDS             7605..8426
                     /locus_tag="IVP39_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7586755.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8711..9721)
                     /locus_tag="IVP39_001682"
     CDS             complement(8711..9721)
                     /locus_tag="IVP39_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7586756.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9733..10686)
                     /locus_tag="IVP39_001683"
     CDS             complement(9733..10686)
                     /locus_tag="IVP39_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7586757.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10676..11746)
                     /locus_tag="IVP39_001684"
     CDS             complement(10676..11746)
                     /locus_tag="IVP39_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586758.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(11752..12795)
                     /locus_tag="IVP39_001685"
     CDS             complement(11752..12795)
                     /locus_tag="IVP39_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586759.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(12796..13737)
                     /locus_tag="IVP39_001686"
     CDS             complement(12796..13737)
                     /locus_tag="IVP39_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586760.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            13821..14168
                     /locus_tag="IVP39_001687"
     CDS             13821..14168
                     /locus_tag="IVP39_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7586761.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14238..15899)
                     /locus_tag="IVP39_001688"
     CDS             complement(14238..15899)
                     /locus_tag="IVP39_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7586762.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4581 a         3329 c         2897 g         5390 t
ORIGIN      
        1 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
       61 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
      121 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
      181 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
      241 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
      301 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
      361 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
      421 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
      481 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
      541 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
      601 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
      661 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
      721 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
      781 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
      841 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
      901 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
      961 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     1021 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     1081 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     1141 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     1201 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     1261 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     1321 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     1381 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     1441 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     1501 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     1561 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     1621 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     1681 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     1741 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     1801 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     1861 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     1921 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     1981 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     2041 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     2101 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     2161 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     2221 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     2281 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     2341 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     2401 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     2461 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     2521 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     2581 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     2641 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     2701 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     2761 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     2821 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     2881 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     2941 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     3001 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     3061 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     3121 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     3181 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     3241 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     3301 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     3361 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     3421 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     3481 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     3541 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     3601 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     3661 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccatcc
     3721 tctttcttct ttgaaatttg gtataatttt cagtataact gagaatattt taatttacca
     3781 ttcttttttc tccactttca gtgaaaaact ccctcttaag acggatgtat ttctttttta
     3841 tttttcgtat actaaaccta ccttttccac gaaggaggaa cagtttatgg attttaagcg
     3901 ttcttatccg aaattagcag attatttatc caaccgggtc gatttttccc gtaaagaaca
     3961 acagcagtcc cattggcatt ggttcacatt aattttattt ggattaatga ctgttgcaac
     4021 gttatcacaa aaactaatat tgctgacttt gttcatcgca atcgttgcat tgatcaaagg
     4081 tcctgtgatg atcttgtggg ggatgttgta ttcctttttg gttgggcttt ttccgcctat
     4141 cggtattttg ctttcagcga ttttcttttt attgaatatc ggaacattta caaagaactg
     4201 gcggatgacc ttagtcggga tctattttta tttctatcct tttggagtga tggcacttag
     4261 tgaagtcatg cattgggata atcattggtt tatagctggc tccttgcttt tagggttgat
     4321 cttgttacac gtgatgttga caaagctcta tcaacattat ggtatcggac gtacgatttt
     4381 ctggtatgtc ttttcgatcc catttgcact gcttactgct ctcttaccaa gccgtttgaa
     4441 gacaaaaata aaaggttacc ataaaatcaa gtgataggat ttttacaaaa taaagaaaac
     4501 aatcatcgaa gtcaaagccg atgattgttt ttttatctat attttcaatc acacatataa
     4561 gagcacaaac taattgaaca taaagtgata gaacttataa ttaacacatc aacgctactg
     4621 ttagatataa atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca
     4681 ataaaaaaga gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt
     4741 ttttctgttt ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc
     4801 gtacttcttt ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc
     4861 ttcaagtaat aagaaggaat ttacgaaaat tgcttctcaa atttcggaaa aagggggtat
     4921 gacacaacta gtgtcatacc ccctttgaat ttctatgcga tttctgagcc atttggcatg
     4981 gattttggtg cttcgattac ttgtaaagag ccatctggtg cttctgcaga aaggatcatt
     5041 ccttgactga tttgtccacg catctttcgt ggtttcagat ttgctacaat gacgactttt
     5101 ttcccgatca attcgcttgg atcaggataa aattcagcga ttcctgacaa gatttgacgg
     5161 tcttggctgt ctccagcatc tagtcggaat tgcagcaact tatctgcccc ttctactttt
     5221 tgacagttga tgacttctgc tacttttagt tctacttttt caaatacatc aaatttgatt
     5281 tgtttttctt tcgttgaaac aagttctgtt tcttctggat cccatttgac tgtatcttcg
     5341 tttgtttgag taccttcaga cattttttct tgaataaaag caacttcttc ttccatatct
     5401 aaacgtggga agattggtgt accttttgca actacttttg ttcctgaagg aaattcgcca
     5461 aagtgtaatc cttcaagatt cattgtttca gagtccaatc ctagttgatt gaagatcttc
     5521 tctggcgttt ctgtcatgat tggctgaagt aatatcgctg cgatacgcaa agattcagct
     5581 aaatggatca tgacactatt caattcagct ttcttttctt cgtcttttgc taatacccat
     5641 ggttctgttt catcaatgta tttgtttgca cgagaaatca gcacccagat ttcagccaat
     5701 gctgtattga attccatttt ttccattgca tcatggtaac gtccgacaac gttagcagcc
     5761 gtcgtagaca gttcactatc aaatggagtg acaagtgagg catagtcagg aacgagtcca
     5821 tcacaatatt tgttgatcat agcaatcgtt cgattcaata agttccctag atcatttgcc
     5881 agatcataat tgacacggga aacaaaatct tctggcgtaa agacaccatc tgacccaaac
     5941 ggaacagcac gaagcaaata ataacggagt gcatctaaac cataacgttc cacaagcatt
     6001 tcaggataaa cgacatttcc ttttgattta gacatttttc cgtctttcat caatagccag
     6061 ccatgcccaa atactttttt aggtagaggc aaatcaaggg ccatcagcat gattggccaa
     6121 tagatcgtat gaaagcgaac gatttcttta ccgaccatct gaacatctgc aggccagtat
     6181 ttttcgaata gactttcatc ttcggagcca tatcctagtg cagtaatata attggataat
     6241 gcatcgatcc atacatacac cacatgtttc ggatcactct tgactggtac tccccaagta
     6301 aaagttgttc gtgaaactgc aagatcctct aatcctggtt tgataaagtt gttgatcatt
     6361 tcgtttttac gagattctgg ctggatgaat tcgggatgtt cttcatagta ttccaataga
     6421 cggtctgcgt atttgctcat gcggaagaag tatgattctt cttttaccaa ttctacttcg
     6481 tgtccacttg gcgctttacc accgatcacg ttaccttcgt catctttgta cacttctgca
     6541 agttgtgttt ctgtaaagaa ttcttcgtca gaaacagagt accagccttc atattcacct
     6601 aagtaaatat ctccctgttc taataaacga tcaaatattt tttgaacagc tgctttatgg
     6661 taatcatctg tcgtgcgaat gaatttatca taactgatat caagcgtttt ccataatttc
     6721 ttgacatcag ccgccatttt atctacatat tcttgcggtt ttacacctag ttcttctgct
     6781 tttgtttcga ttttttgacc atgttcatcc actccagtga gataaaacac atcaaagccc
     6841 atcaaccgtt tataacgagc aattgcgtca catgcaatcg tagtatacga gttgccgata
     6901 tgaagtttgc cgcttggata atagattggc gtagtgatat aaaaagtttc cttttctgcc
     6961 acggggctct tcctccttta tcgcatgatg cgaattcgtt tccttgctag tataccataa
     7021 aaaaagcatg ctgttagctt tttttcaaaa tgaaaaagac tggataaacg acttctatct
     7081 acgttcaatc tatcctttaa aaggatgaat aggttgaata aatgcgggtt tttgttttgt
     7141 ttcatatcta ctaaaaaacg gctttttatt tctcccctca cacgtggtat aataaagact
     7201 atctatctta gtaagggact ggaacaatca tgcagaagaa acatcttttt tttacactta
     7261 gtattgcttt tctttcatta gcacatttaa tattcagcta tttttatatt cgtatgtatg
     7321 gttattttaa cctccatggg catttgaaca gttttatgac agcagcttgg attttgcgtt
     7381 ttatcattga cgtatatatc gttatttgtg gcttctttgc cattcgtgaa gagcggtata
     7441 aagtattgcc gttttatctg cttttctttt tattcaatct catacttcca tttatttttc
     7501 atatttaaat gataacctag atttttcaag taaatccttg gttttttttc agagtctgct
     7561 acaataaggt aaaaagtact tttactgtca atggaggtgg tcagatgaac cgttttgctt
     7621 ctaaagaaga agaaaaatta tattatgaac ataaagcaag tgaagaagaa tttcttaact
     7681 ggtatcaaaa acaggaacgt ccagaatatg acaagccctc tttgactgtc gatatcgtac
     7741 tgatgtgcta taacaaagaa gaagatcagc tgaaagtttt gttgatccaa agaaaagggc
     7801 atccttatcg aaattcatgg gcattgcctg gtggctttgt ccaaaaggat gagtcaactg
     7861 gagaaagcgt cttaagagaa acaaaggaag aaactggtgt ggttatttca aaagaaaata
     7921 ttgagcaact gcatacattc agtacgccta atcgagatcc gcgaggctgg gtcgtcacag
     7981 tcagctactt agcctttatt ggagaagaac cgctaatcgc cggagatgat gccaaggaag
     8041 tacgctggtt cacactagaa cgtcatagta acaagatcca tttatctagc ggagaagtgg
     8101 ctatctcgct tgatttagtc actggagagt cttctggcaa agatactttg gcttttgacc
     8161 atagtcaaat catcttgaaa gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc
     8221 aagttctaca agttttaggt aaagatttca cgattacgga agctcgaaaa gtatttgcta
     8281 aattcttagg gatcgattat aaaacgatcg atcattccaa ttttaagaaa gctatgctcc
     8341 aatatttcga tgagatcggt gaacgtcctg taggaatcgg ccgtccttca aaaatctatc
     8401 agctcaagcc ggggcaccat gaataaggtt gtgagcaaaa gattcagacc taatcattca
     8461 agaaaaaacg caaaaacaac tctttgtgag ttacgttttt tgtttcagaa ttttgatgta
     8521 ttcgtaaact ttctgaataa aaaattaaca atagcagaaa aagctcgtac gaactttttc
     8581 tgctattgtt ttattggttt gtcataatta gtgagctttt cttcaaaaga aaaattccta
     8641 gaaacaaaag tcattaagac tttttattaa caaggctaac cctaacgctc ctatcagttt
     8701 cattcatttt ttatcccttg ataaccgaat gtctgcccat tttttctact acttggctct
     8761 ttcttacgga attgctttct tctgctccgt cggtattgat caaaagaaat tttttattat
     8821 accattggga cttataacgc aattttgtta ttaatttatg ccagtcgtga atagaaaaat
     8881 cagcatgtgc ctcttctttt ggaaaagatt ctacaaataa gtagtcatat tgtttgacca
     8941 atttcccaga taattgttca agataatctt ctcgctggtt cttttgtttc agataaagat
     9001 ccattacttt tctcttttgt ttctgataat tgcttgcctg atctaacctg atctttctat
     9061 ggtgagcgga gcggactttc acattcaatt tccgctgtgc atgttgcagc ttttcttctg
     9121 tctggtgaaa ttttggtaaa gttacttcta gaggtcgaga tgtttggatc aatgtcttcg
     9181 gatgataggc gatcccgatc catttagcta aagattcttt ttttactggc gaaacatcac
     9241 aaagtagtgc gacataaaat tcttcattat atcgagcaga aatcgtagcg gagcgaatcg
     9301 atccttcaac tggccgatgc tgatttatct tgattagttc tttttgtttt ggtaacttta
     9361 agtagccatt ttccagatag atcgtacggg tttggttgtt ggtcgtataa gattgccaca
     9421 tacttttttt cgttttaagc tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag
     9481 cccgatccaa attaagctgt gcatttgcta aagcaagact gtctacttct tttaagaatg
     9541 ggtattcttt ttttaatgta gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc
     9601 aacttttctg ccgctttttc agcaaatggt tataggtaaa tcgagcacat ccaaaagtac
     9661 gaatcaattg ctgtttttgt tcttctgtcg gatacatacg gaatttaaaa gcttttagca
     9721 tctatgtccc tcctagttag caaccagaag agattccttt tgtcgtttta gtttttttgc
     9781 gtaatatggg acttcctgct cagttgcata gacaaaatgt tctggtgcga tttcacgaag
     9841 ttgacgtact tgtccgtcat ctggttcagg ttgatatttg attcttctgc gttttctctc
     9901 atggtctgga tctggtagag gaatagcaga taacaaactt tctgtataag gatgaacacc
     9961 gtgattatag atttcatcac ttgttcccat ctctaataac aagccgttat gcattacacc
    10021 gattcggtca ctaatatgtt taaccatcga caaatcatgt gcaataaata gataggtcaa
    10081 ttgatgttct ttttggagat cttgcaacaa attgaccact tgggcttgga tcgacacatc
    10141 tagtgcagaa attggttcat cacaaatgat aaaacgcggt tttactgcca atgctcgtgc
    10201 aataccaata cgctggcgtt gcccacctga aaattcatga ggataacgag taccgtggct
    10261 tggattcaat ccaacagttc tcaatagatc atcgactttt tttgcacgct cttcagatga
    10321 tttagctagt ccattaacat caattccttc ggcaatgata tctctgactt tcatacgagg
    10381 attcaacgaa gcgtatggat cttgaaagat catctgaaca tcatgccgga atttcgtcaa
    10441 acctttttta tctttgatct tcgttatatc ttgaccgtcg aataatatct caccatcagt
    10501 aggttcattc aagcgaatga tcgtacggcc agtagtggat tttccgcttc cagattctcc
    10561 aactaaaccg aatgtttctc cttcataaat gtgaaagctg atgtcattta cagcatgtac
    10621 ttcattcttt tttcctacat tgaaatactg tttcaatcct ttaacttcaa gtagtttatt
    10681 tggcatcagc aattcctcct tgaccgtaag tatgtttttg tttctcttta aagatagccc
    10741 agcgacgttg gatttcttct ggcggtgtca ctttgggtgc ctgcggagca agtagccaag
    10801 ttgctgcctt gtgtgttttt gatagttcaa aaaatggtgg ttcttgttct gcatcgattt
    10861 tcatcgcgaa ttcatttctt ggatagaatg cgtctccttt tggcgggttc aataaatccg
    10921 gtggagaacc aggaatagca tacaatcgat cgttcgcact atctaatgtc ggcattgaac
    10981 caagcagtcc ccaagtatag ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa
    11041 cttcaacgat tcttcctcca tacatgacag cgacacgatc agcgacattc gctactacac
    11101 caaggtcatg agtgataaag ataattgatg tatcgatttt tgtctgaatt tctttcagca
    11161 gctccaaaat ctgtgcttga atagtcacat caagcgctgt tgtaggttca tcagcaatca
    11221 ggatttccgg ataacatacc agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg
    11281 agaactgatg cggataactg ttgatacgtt tctcagcttc gggtattcca actaatttaa
    11341 gcaattctaa tgcagctttt tgtccctctt tttttgaaac tttattatgc tttctaaggg
    11401 actcagcgac ttgcttccca atgggcatcg tcggatctaa agaggtcatt ggatcttgga
    11461 aaatcatcgc tatttcttta ccacggattt tttgcatctc tctttctgat tttccaacga
    11521 tatcgttacc tttaaagagg atttgaccag catctatatt ggcgttgctt gacagcaagc
    11581 gcatgatgct tctggtcgtc actgattttc cacttccaga ttccccaacg atcgccaatg
    11641 tttctccttt attcaaatga aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg
    11701 tatcaaaaga gatttctaag tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt
    11761 cattttagga tcaaaggcat cacgcaatcc atcagcaagt aagttaaagc caatcatgat
    11821 aaccgataaa gttactgccg gaatccataa tagataaggc agatacagga atgtcttgta
    11881 cccttcgctc aacatcgttc ctaatgaagc tgacggaggc gtaagaccta aaccgataaa
    11941 gcttaaaaag gcttcgaaga aaatagcaga tggaatacta aacatcattt gaataataat
    12001 gacactagag atattcggca aaatatgttt gaatgcaatt ttcattcttg attcacctaa
    12061 tgtcaatcca gctaaaacat attcttgatc ttttaatttc attgtttgtg cacgaacgat
    12121 acgtgccatt ggtatccagt ttgtgatggc catagcagcc acgattgaaa aaattcctgg
    12181 ttcaaaaacg accaacatca aaatcatgac aacgaggttt ggaattcctg ataagatttc
    12241 caaaaaacgt tgcatcacgt tatcgacacg tccgcctaac aaaccagaaa ccaacccgta
    12301 agctacacca atcgttacat ctagtaatgc agcaataaat gcgatcagca acgagattct
    12361 cattcccata aataagcggc ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa
    12421 atttacgttt gaagggacgt ttgcttgagc atatttatca acaagttcac caccaaccat
    12481 tgcttttcca ttcaatccat cgatattgat tcccggaata cgaggaggca aattgatata
    12541 agccacgttt tgggcagttg gatcatgagg agaaacaaag attgtgatca cagagatcaa
    12601 aatgatgacc aatagtaaaa ccattgaaat aacggcagct ttgtttttct ttaaacggcg
    12661 ccatgaatct tggataaaat tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt
    12721 atttttttgt aatggaagaa attcatccgc agggatatct gcaactgttt cataacgctt
    12781 tggtatcatt tccatttatc ctcggctccc ttctgaaaca cgaattcttg gatcaactag
    12841 accgtacaat agatccacga tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt
    12901 tacagccatg atcgtaggat aatcatttgt tgtgattgac ttaacgaatt gttccccgat
    12961 accaggaata gcaaagatat tttctaccac cagtgatccg gtcatcaatg ctacagctaa
    13021 cggtcctaat aaagtcatca atggaatcaa actatttcga agaccatgtt taaaggcgat
    13081 ttcccaacgg ctcaatcctt ttgcttttgc taattccaca taatcactat gcagtacttc
    13141 caccatttcc gtacggataa accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat
    13201 tgttggtaag attgtgtaca caaagccatc ccattttgcg attggcaaaa cttgtaattt
    13261 gattgcaaag atatattgta ataaaacagc aaatacaaag tttggaatgg aacgacctaa
    13321 tatcgctacg agtgtagaag atgtatctgc ccatgtattt tgcttcatgg cagagatcgt
    13381 tcctaaaatc gtccctagga ctgttccaaa gataattgcc tgtaacccta actgcaagga
    13441 tggtccgatt cttccagcta acaaatgtgc gactggctgg tttttaaatt ggaaggaaat
    13501 tccaaaatct ccttgaagca aattagaaag ataaatccca tattgtacga taactggttt
    13561 atccaaaccg acttgtttat ttagcatctc gattgtttca ggactcaaac gttcctgatt
    13621 cgtatatggc gtgcccggca gcaattgcat caagaaaaag gtgattgttg caattaacca
    13681 aagcgtgatg atcatgaaaa agacacgttt taatacatat ttgatataac tattcactaa
    13741 tgtttccccc tagcttttga cttctgacag atgtttcgct acattattag ataatgaacc
    13801 aaaaataaag gagcaatcaa atgaaaaaaa aatctatccc ctacgcagta gcttttctac
    13861 tgattctggt tattttaata aaaaatctca tcaaccattc ctttacactc attcaattat
    13921 ctaatgatct attcttatgg tcattgccgt ttctgattat cggcggtttt ttatgggtct
    13981 tttcttcggg tttcttcgat cattttcagc gatcggtcca tcttgcacga acaaggaacc
    14041 gaaagaaaaa accagaattt tcctcgctct cgtctgccag ctacggtatg tacagctttt
    14101 ggctgatcat tgccggaata ctaattgctc tttctgctat tttcatgctt ttttctttgt
    14161 taggataagt gaatatgaga acgaggttgc gacatttgcc gcagcctcgt tttttctcat
    14221 gctcacggta ttaacgatta ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct
    14281 gccgcatggt gaacgatacc tttgattttt tcattcacta ggtgaccttc tgctttttgg
    14341 tacaccggaa cgacacccat atcatcagag atgatcttga ttgctttttg gaaatcatcc
    14401 caacgggctt gttcatcgcc agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat
    14461 tctgcattgc tccagcgacc acggttgtat gaattacctg tgacaaacaa atcagtaaag
    14521 ctgcttgggt ctgcatagtc agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa
    14581 gttgagcggt caagacgtac agagaatgga acaggtgttg gtttgactgt tacgccatcc
    14641 aagttttctt ggattgagtt ttgaacatat tcgattactt ttttcgatga atcatcatca
    14701 gaagccatca aatcaaattc taaagaatca acgcccaatt cttttttcgc tttttcccaa
    14761 tattcttttg ctttatcttt gtcataagaa actaattttc cagcttcttt tgcaaagtct
    14821 tcgttcgttg ttggactttt taccatatct gatgggatca atcctgtaga gacaactgat
    14881 ccgtcaccta gtacttgttt tactaaagct tcacgatcga tagaataaga aatagctttt
    14941 cttaggttca cattgttgaa tggtgaattc ttttcacgtt gattgaattc gatatagctt
    15001 gtccgcgctt ctttaacaga agtataagct ggatcgtttg cgttttgctg tgctaactca
    15061 ccagacaaga tcacgtcatc tgcttggcca tctttgaata agttcaatgc agtagaagat
    15121 tcttttacta cactgacttt gatttcagaa agttttactg tatctttgtc ccagtaatga
    15181 tcatttttct tataagtcca ttctgtatct gttcctggtc catcaaaatc ttctaatgtg
    15241 aatggtccat tataaacagc tttatcactt gcagatgcat aggcatcccc attttcttcc
    15301 actacggatt gtttttgtgg gaaaaatgat gggaaagcaa gcagatattg gaaatatggt
    15361 gtttgtttcg tcaatttgat ttctaattca tagtcaccaa ctgctttgat ccctagttct
    15421 gatttatctt tctttccagc tgtaatgtct tctgcatttt caactggcgc atacaggtaa
    15481 gcatattcag aagctgtagc tgggtctact gttcgttgcc agccataaac gtaatccgct
    15541 gctgtaacag gatcaccatt tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt
    15601 aatccgtctt cactgacttc tgccaactcg ctagctcctg ccggctgtgg tttgctgtct
    15661 tcatcaagac ggtaaattcc ttcataaacg ttattcaatg ctgtaaaact gattgtatct
    15721 gttgcaactg ataagtcagc tgtaggcatt tcttgaggca cgactaagtt aaatacgcct
    15781 cctccatctg ctgaaccgct ttttgctgaa ctactgcttg tggctgaact gccgccataa
    15841 catccagcaa gtacaaggcc gcataccgca accaaaccaa atgtaaattt ctttttcatt
    15901 ttacgtaccc ctttattgga attttcagaa taattaatat tttctgaagt tgtttaagag
    15961 tttaatgaat aattattaaa aaatcaacac ttttttttgt ctttttcata aaataatgat
    16021 tttttcgtga ggtttcaagg aatttttcag aaaaaatagc tttgtacaag gttttccatg
    16081 gtcttccaaa agccattaaa taattttcgt tatattgtct attattgcca tacaaagata
    16141 aaaaagaata aacaaaaaga aactgtgaca aaagtcttgt cacagtccct tatctga
//
LOCUS       DACCSK010000060        16143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCSK010000060 DACCSK010000000
VERSION     DACCSK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.061"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(182..601)
                     /locus_tag="IVP39_001689"
     CDS             complement(182..601)
                     /locus_tag="IVP39_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7586763.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(603..1187)
                     /locus_tag="IVP39_001690"
     CDS             complement(603..1187)
                     /locus_tag="IVP39_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7586764.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1234..2709)
                     /locus_tag="IVP39_001691"
     CDS             complement(1234..2709)
                     /locus_tag="IVP39_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7586765.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2890..3201
                     /locus_tag="IVP39_001692"
     CDS             2890..3201
                     /locus_tag="IVP39_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586766.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3316..5652)
                     /locus_tag="IVP39_001693"
     CDS             complement(3316..5652)
                     /locus_tag="IVP39_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7586767.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5794..6462
                     /locus_tag="IVP39_001694"
     CDS             5794..6462
                     /locus_tag="IVP39_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586768.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6465..7508
                     /locus_tag="IVP39_001695"
     CDS             6465..7508
                     /locus_tag="IVP39_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7586769.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7745..8626)
                     /locus_tag="IVP39_001696"
     CDS             complement(7745..8626)
                     /locus_tag="IVP39_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7586770.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8785..9513
                     /locus_tag="IVP39_001697"
     CDS             8785..9513
                     /locus_tag="IVP39_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7586771.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9669..9986
                     /locus_tag="IVP39_001698"
     CDS             9669..9986
                     /locus_tag="IVP39_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7586772.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9979..10587
                     /locus_tag="IVP39_001699"
     CDS             9979..10587
                     /locus_tag="IVP39_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7586773.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10603..11433
                     /locus_tag="IVP39_001700"
     CDS             10603..11433
                     /locus_tag="IVP39_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7586774.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            11571..12059
                     /locus_tag="IVP39_001701"
     CDS             11571..12059
                     /locus_tag="IVP39_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7586775.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12110..12982
                     /locus_tag="IVP39_001702"
     CDS             12110..12982
                     /locus_tag="IVP39_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7586776.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13158..13520
                     /locus_tag="IVP39_001703"
     CDS             13158..13520
                     /locus_tag="IVP39_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7586777.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13529..14281
                     /locus_tag="IVP39_001704"
     CDS             13529..14281
                     /locus_tag="IVP39_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586778.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14295..16097
                     /locus_tag="IVP39_001705"
     CDS             14295..16097
                     /locus_tag="IVP39_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586779.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT         5025 a         2811 c         3306 g         5001 t
ORIGIN      
        1 ccatgtttac aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga
       61 taaacggcgt tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag
      121 ctatttttcc atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct
      181 ttcataatgg tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt
      241 tttccaacca atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga
      301 tttgtgcatt ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa
      361 cggcagtttt gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac
      421 catcgtggct agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg
      481 ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt
      541 taacagcagc ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca
      601 tattaatcac cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt
      661 ttcattgtat ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga
      721 attggtggta tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg
      781 gcatttaatg gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca
      841 tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc
      901 atacttgccg gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca
      961 cctaaatctt tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag
     1021 tcggattcca gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc
     1081 atcggatatt cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca
     1141 cttacaccaa tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct
     1201 ttttatgatg tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc
     1261 tacttcttgg aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc
     1321 aaagatagtt tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa
     1381 ttctgatggg tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc
     1441 ttggaaacga gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc
     1501 atcgactaaa atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg
     1561 acgctgaata ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt
     1621 accaccacct gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac
     1681 caaggcaaga atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg
     1741 accaatcgtt gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac
     1801 tacttgtaca gctggattag catccccagt atagccagga aattctggca ttgcttttcc
     1861 tgagttcgtg ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat
     1921 gaattcagca cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg
     1981 ttcgttacgc aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg
     2041 ttcgaaagtc gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat
     2101 tggtttattc aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat
     2161 gatgtacata ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct
     2221 cattgttttt tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc
     2281 ttcaggagta tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc
     2341 tagatgaacg acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg
     2401 attttctaca gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc
     2461 acctacacgt ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt
     2521 gatattgttg aaagtcatag ctggtccttc ttgagtcgga cgttttactg taccgccagc
     2581 accgatataa cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc
     2641 gtgatattga cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg
     2701 atctgtcatg agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa
     2761 gaggaaaaca attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt
     2821 gataatagaa taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga
     2881 ggaaggaaag tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata
     2941 gcgaatattt tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg
     3001 acagaagaga taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat
     3061 cttctcgcac tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga
     3121 acagtattca taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag
     3181 aaaagtatac cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat
     3241 tacttcctcg tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat
     3301 gtggttgtca caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat
     3361 tcgtgcagga agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat
     3421 cgcatggaca ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct
     3481 attgataggg aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc
     3541 taaaatacac gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc
     3601 ttttagtaca ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat
     3661 catgatcata cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc
     3721 atccatcaat ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt
     3781 atcttcatct gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc
     3841 ttcaaaattg ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact
     3901 aagcaatgtt tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact
     3961 gtctctttga gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa
     4021 ttgatcgcta taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg
     4081 taggatcccc gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt
     4141 attagggata aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt
     4201 gaatgttgta ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc
     4261 aagattcttc aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc
     4321 tgttgctgag gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc
     4381 tttgctagtg tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat
     4441 cgtactagat aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg
     4501 tactttgcca tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt
     4561 attacttaag ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt
     4621 cgtgtgctgt gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata
     4681 atccgctttg cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga
     4741 aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa
     4801 tccagaagaa agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga
     4861 ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt
     4921 ccaaaaactc atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg
     4981 aggatgggaa tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg
     5041 atgggccagt tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg
     5101 gatcaattcc atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca
     5161 caaaaggata tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc
     5221 accagataac tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc
     5281 atcttctgcc cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc
     5341 aacgttttca ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat
     5401 agagttgttt cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt
     5461 gattaccata tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt
     5521 tttaccagaa ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga
     5581 cacaccatcc aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt
     5641 taattgaagc ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat
     5701 cagaacaatg atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa
     5761 tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat
     5821 gatccgcaat tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc
     5881 accgcattga atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc
     5941 attacagata tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc
     6001 accagatttg acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa
     6061 cgcgggttca aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg
     6121 atcctacgtg tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata
     6181 atcggacaaa caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg
     6241 attctgcctc aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc
     6301 ttcactcgcc aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga
     6361 acgatcgatg tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa
     6421 atcatcacag tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat
     6481 tttcacaatt atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc
     6541 tgtgttttct cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg
     6601 ctggaaaacc aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta
     6661 caggaatatc gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa
     6721 atatgagtct ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag
     6781 cagaagtaca gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg
     6841 agacactgag gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa
     6901 caatccaagg atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa
     6961 tatttttata tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct
     7021 taaaattgaa caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag
     7081 atgaacaaat cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag
     7141 aattggatat cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag
     7201 tgtggctgaa tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata
     7261 tcaaactatt tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa
     7321 tgaatgaaga gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc
     7381 aaatttctgg gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg
     7441 gacgtattga ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta
     7501 aacaatagga aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta
     7561 gtcgtttttt tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct
     7621 ttcttattga actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa
     7681 agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta
     7741 gggcttagtt ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct
     7801 caccgacttt acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca
     7861 cagtattttc ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt
     7921 catttgcaat catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt
     7981 caaatccagc ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa
     8041 ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag
     8101 gatgattttt aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg
     8161 aacttggtgg ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt
     8221 cattgtaagt taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc
     8281 gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta
     8341 agatatttaa tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat
     8401 ggtattgttc tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt
     8461 cgatttttct atgggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa
     8521 tctgtttaga aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca
     8581 ttgtttcggc taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac
     8641 ttattccaat aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt
     8701 tacaatcttt cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc
     8761 gtagaaaaag agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt
     8821 ttgtttggcg gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca
     8881 cagctgacga tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg
     8941 atgaaaaata tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt
     9001 gttcatttag gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa
     9061 atcttcccta gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg
     9121 gtcattgttc tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg
     9181 acaggagaca gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt
     9241 tttgcgtgta atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt
     9301 tatattttgt tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta
     9361 gattttaaag cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg
     9421 aaaatgttcc cagtagctga catgatacct gcaatgatcg ttattatgcc actgagctgg
     9481 atgtggacaa attggattat gccactgtta taataaaaat caaagagaag ccattttttg
     9541 ctgactaaaa aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga
     9601 aactatatta tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag
     9661 gtgacgtaat gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat
     9721 taagcaagga agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg
     9781 tcagtgaatc tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga
     9841 cctatgatga accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa
     9901 gtcgtcattt agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt
     9961 tattggagga gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa
    10021 attcctgaag acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct
    10081 ggaaaaacat tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa
    10141 ttaaaaatca gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca
    10201 aaaagacaaa ttcgcctgat ttggttaatc attatcggtt tatttgcttc tcctgtcctc
    10261 attccactag cactggggtt tatattggtt attgggggac tattggttgc tttactagcg
    10321 ctcattttta gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc
    10381 atctcaggaa tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc
    10441 ggtctgttcg ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa
    10501 gggctttttt atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa
    10561 agagatctgc attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt
    10621 ttgattaccg gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca
    10681 ttaggcgctc aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact
    10741 gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt
    10801 tcggtcatac gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca
    10861 gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca
    10921 atcattagtt tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa
    10981 ttatctgaat tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc
    11041 aaaaaagtga atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga
    11101 acaataactg cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac
    11161 ttgatcgtca caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa
    11221 ggaaaagtca tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat
    11281 actatccaaa cgtctggtga atcttatatt gaaaaagact accagccaat aacaaatact
    11341 ttgttagaac aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt
    11401 acgacggatc aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga
    11461 aaaaacagca aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat
    11521 agtaaaataa gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag
    11581 aagaaggtca aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc
    11641 aactaagcca aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca
    11701 attcagaagg tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg
    11761 tgaagaaaga gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg
    11821 atgggaagaa ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag
    11881 ggatggatct agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa
    11941 caaaattgga ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca
    12001 ctgaagacaa agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa
    12061 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12121 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12181 tggattgttt ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag
    12241 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12301 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12361 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    12421 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    12481 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    12541 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    12601 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    12661 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    12721 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    12781 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    12841 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    12901 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    12961 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13021 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13081 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13141 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13201 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13261 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13321 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13381 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    13441 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg
    13501 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    13561 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    13621 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact
    13681 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    13741 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    13801 tattttccaa gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    13861 tctttcttta caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    13921 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa
    13981 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14041 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14101 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14161 ttgccaacgt attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga
    14221 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14281 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14341 actacttggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    14401 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    14461 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    14521 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa
    14581 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    14641 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    14701 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    14761 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    14821 tcaaaaaacc gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    14881 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    14941 atatgatgaa acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga
    15001 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15061 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15121 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15181 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15241 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15301 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15361 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    15421 cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag
    15481 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    15541 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    15601 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    15661 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    15721 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    15781 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    15841 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    15901 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    15961 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat
    16021 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16081 tgaaaaagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa
    16141 tag
//
LOCUS       DACCSK010000061        16098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCSK010000061 DACCSK010000000
VERSION     DACCSK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.062"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(297..3575)
                     /locus_tag="IVP39_001706"
     CDS             complement(297..3575)
                     /locus_tag="IVP39_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7586780.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3846..4391)
                     /locus_tag="IVP39_001707"
     CDS             complement(3846..4391)
                     /locus_tag="IVP39_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7586781.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4403..5095)
                     /locus_tag="IVP39_001708"
     CDS             complement(4403..5095)
                     /locus_tag="IVP39_001708"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7586782.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5114..5398)
                     /locus_tag="IVP39_001709"
     CDS             complement(5114..5398)
                     /locus_tag="IVP39_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586783.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5411..5974)
                     /locus_tag="IVP39_001710"
     CDS             complement(5411..5974)
                     /locus_tag="IVP39_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7586784.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6202..6864
                     /locus_tag="IVP39_001711"
     CDS             6202..6864
                     /locus_tag="IVP39_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7586785.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6988..8184
                     /locus_tag="IVP39_001712"
     CDS             6988..8184
                     /locus_tag="IVP39_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7586786.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8285..9661)
                     /locus_tag="IVP39_001713"
     CDS             complement(8285..9661)
                     /locus_tag="IVP39_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7586787.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9698..10564)
                     /locus_tag="IVP39_001714"
     CDS             complement(9698..10564)
                     /locus_tag="IVP39_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7586788.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10653..11678)
                     /locus_tag="IVP39_001715"
     CDS             complement(10653..11678)
                     /locus_tag="IVP39_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7586789.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11696..12370)
                     /locus_tag="IVP39_001716"
     CDS             complement(11696..12370)
                     /locus_tag="IVP39_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586790.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12554..13447)
                     /locus_tag="IVP39_001717"
     CDS             complement(12554..13447)
                     /locus_tag="IVP39_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7586791.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13521..15218)
                     /locus_tag="IVP39_001718"
     CDS             complement(13521..15218)
                     /locus_tag="IVP39_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7586792.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSKWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15211..16065)
                     /locus_tag="IVP39_001719"
     CDS             complement(15211..16065)
                     /locus_tag="IVP39_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7586793.1"
                     /translation="MKAPKKNQDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPI
                     YHPTESTEELQEIHIYPKNVGYLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGG
                     KYYLDMAPANDLKNQWIKPADNDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEEL
                     KGVEGYEMDEASKRIEVIIPEFWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATP
                     RVYNETVKETTDKPKEPSGPSNPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIK
                     KERGEKNE"
BASE COUNT         4604 a         3517 c         2804 g         5173 t
ORIGIN      
        1 agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga aaaatagttc
       61 ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt
      121 ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaagcagc
      181 aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg tctcatttcc
      241 ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa ttccacttat
      301 ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc tccagctaat
      361 gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc atttttttca
      421 ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc atttggtgat
      481 tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc ggcagtttct
      541 cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca tttttcccca
      601 tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga aggtgctgaa
      661 aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat ttggatcttt
      721 tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt gaaagttgac
      781 acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc cgccacacca
      841 tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg atttggttct
      901 tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc aaaaatagca
      961 cctacagata atggaagacc atccactctt tccttttctg tttgacgatt ccccggttta
     1021 atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac aggctgctga
     1081 ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt atgtccaaga
     1141 gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg gtagatttga
     1201 tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc tgttcggaaa
     1261 gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata aagagccaca
     1321 ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt tccgctattg
     1381 gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc atgagggccc
     1441 gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc aattcctggt
     1501 acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat cgaacctgtt
     1561 tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat acaaccgtca
     1621 aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc ttcaaatcct
     1681 ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg ccatgcttcg
     1741 aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg ataagcatga
     1801 atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat agctgcacta
     1861 tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat cccatttcca
     1921 gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc aatcaactga
     1981 atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct ttcgaaggaa
     2041 taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa tgcttgtgtc
     2101 agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg tgttacataa
     2161 actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt tgtttcataa
     2221 ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa actgaaagta
     2281 aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt cgcagtgata
     2341 gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat tgtcaataat
     2401 ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg attactagag
     2461 gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg aggatctgtc
     2521 gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt ccttgctgca
     2581 gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca gtcatacatg
     2641 atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc taaaccagta
     2701 ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg aaacagattc
     2761 ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa ccctgctgaa
     2821 gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc ttgcggatct
     2881 tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt atatttgctg
     2941 ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat ccgactgcct
     3001 agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt tggtactgcg
     3061 tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga ttctatttcg
     3121 atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata ataggtcatt
     3181 ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag ccgcacgccg
     3241 tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa aacaacccct
     3301 ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc tgattctttc
     3361 atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg tatttcctcc
     3421 attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga ctgttgttcg
     3481 ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag gagattcatc
     3541 attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt aacaaattag
     3601 aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa acaaaattat
     3661 aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat aaagagattt
     3721 attaacggaa aaagaataag tttttttaaa ataacgtata aaaaagagca taaaacaaag
     3781 tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca aatcactttg
     3841 tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt
     3901 ccaacgggat cgaaacttgc cacggaatct ttgaaaatat ggagtttgta tcctagatta
     3961 tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt caaataaata
     4021 tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa agcggaataa
     4081 tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta taaacttttt
     4141 aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg aaataagcgg
     4201 ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc
     4261 ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac ttgtcctgct
     4321 gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc aatcgagatc
     4381 aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc accatttctg
     4441 ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg
     4501 ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga gctgtttgtc
     4561 caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt aagatttctt
     4621 tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct tcttttgcgt
     4681 tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg cttgcttcat
     4741 aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt gcatctgcat
     4801 caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct tcaccgattc
     4861 cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc aacagacttg
     4921 tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact tcgtggttac
     4981 ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt tcgcttaggt
     5041 tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt ttcatctttt
     5101 taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac attaggataa
     5161 caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc cgttcgctga
     5221 tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag ctggcaatct
     5281 ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat gactcgcttg
     5341 cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa
     5401 gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca taaatcgtct
     5461 ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga gttagatgat
     5521 acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt
     5581 gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac atcgaagtaa
     5641 gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta gctgctgtca
     5701 tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc aaaatcactt
     5761 tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa
     5821 tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct ccatctggca
     5881 aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct ttgcgactga
     5941 ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc tttgtaatca
     6001 taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt ttaccataga
     6061 taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac tctttctagt
     6121 agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat actcaacgat
     6181 aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct agcattggtc
     6241 ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact tttattgata
     6301 ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa
     6361 gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg tatgacagcg
     6421 aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat caatcaattg
     6481 caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca tgatccagtc
     6541 aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact gcatgaagca
     6601 agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa gtataatgag
     6661 atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga aagcgcgcag
     6721 atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt tgctgaggat
     6781 ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg tgataataaa
     6841 tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg gaacattcag
     6901 atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt aagaatcgag
     6961 aacagattca acaaggagga attttttatg gatcataaac cgactgaaac aaaagatgtt
     7021 acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac tccaacagat
     7081 acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca gacagctgct
     7141 cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct agcagcaaaa
     7201 atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca agcaaaatta
     7261 agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg tcctgtagga
     7321 gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga actgcgtgcc
     7381 ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg
     7441 acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa attagataaa
     7501 gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca gaaaaacaaa
     7561 gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat ggaagaactt
     7621 caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga tcagatggat
     7681 gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg tacgcatgat
     7741 cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt gatccaaaac
     7801 acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat tccgctgtgg
     7861 aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt aactgctcaa
     7921 cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat gctgaaaatc
     7981 tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga gacccttcaa
     8041 accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca aaaagaagga
     8101 aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga tctgaaacaa
     8161 aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca aacgcccaac
     8221 aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac ttctccattg
     8281 gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa atgatcagtc
     8341 caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga agcatcaata
     8401 caatacaagt caagatggag acgattggca atagcggaac gaatggcact ttgaattctc
     8461 cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta acaagcatca
     8521 aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg aaaaatccgg
     8581 caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta cgatgctttt
     8641 gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca aacagtaaac
     8701 gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt gcccctacag
     8761 cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc actgggtcat
     8821 tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa
     8881 ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt tgcgggtttt
     8941 tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa aacacaagag
     9001 ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt
     9061 taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg acatatacca
     9121 tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa gtgacgaata
     9181 tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg tacccgccag
     9241 ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata tagccagacc
     9301 atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac caccctgtca
     9361 gccaggcagc aaattcacca aatacaacat acagataggc atatggccca ccaatcactg
     9421 gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg attgcagcta
     9481 agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc cctgtcacca
     9541 caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca gacgttttta
     9601 gttctttttt catctcactc gtatgattga gattgatctc tttcttacga aacaaatcca
     9661 ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc atatacccta
     9721 aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg agctcttttt
     9781 tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg atccccattt
     9841 gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc atgcagatgt
     9901 ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga tcgactttaa
     9961 ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga ggtaagataa
    10021 ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca taaccaatcg
    10081 tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta tctgtatcta
    10141 tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga agagctaaag
    10201 caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa acggctcccg
    10261 caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca gaaacaccca
    10321 tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag ccggataaaa
    10381 atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag acaactaaag
    10441 aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg ccaccaattt
    10501 taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct attaaaattt
    10561 ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa acgttaacaa
    10621 cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata aaaatttaag
    10681 attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc cgtcaaatgg
    10741 atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt tgaatgtgtt
    10801 ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt caagtcttcc
    10861 gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc ttccttttct
    10921 atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact aagcaattgg
    10981 ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc accaacgatc
    11041 tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac aggctgaatg
    11101 attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc gagtcttgca
    11161 taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc tagtaatgta
    11221 tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga agcggaaagt
    11281 ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg ttgattactg
    11341 attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata ttcttctacc
    11401 agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga agttttttgt
    11461 atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag cagataaaaa
    11521 atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc aactttgatg
    11581 tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca tcccaagatc
    11641 aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca tcctttcagg
    11701 gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct gcctgttcga
    11761 tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca aatcgttcgt
    11821 catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg tgcttcatca
    11881 aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg ttgatttcca
    11941 tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca ctagataatt
    12001 ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt acatcgattt
    12061 ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc atatccatat
    12121 tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt attttttgac
    12181 accaataata gccatccaaa acgggaagat taatatctag taatacaagc tgtggttctt
    12241 ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt tcaaattgcc
    12301 atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg actaccatga
    12361 tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca aagaagccct
    12421 tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa aaaaactgga
    12481 aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta tgttattctt
    12541 aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta aattttcgat
    12601 aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt ataagtaatc
    12661 gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct gtaaagggaa
    12721 ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta caagtgataa
    12781 gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa
    12841 caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga tagaatcggt
    12901 catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata ccgctatggg
    12961 cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct tgcaggactg
    13021 tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag ctaacattga
    13081 ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt cctggatttt
    13141 ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct gggattactg
    13201 atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc tcatgttttt
    13261 cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga tccaaataat
    13321 tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca agaaacatca
    13381 ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta cctattttct
    13441 ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata aaaccgattg
    13501 ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg gtaccagctg
    13561 tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc gttcccacca
    13621 gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg attttctact
    13681 cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc agtaaaggct
    13741 gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat ttgataggtt
    13801 ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct
    13861 tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc taaaggcgac
    13921 tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc gaatcttgca
    13981 tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc acctgttgtg
    14041 actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg tccgatttca
    14101 ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta
    14161 tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt atctactttg
    14221 gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac tacttctact
    14281 atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat ttcctgatca
    14341 taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat aacagcgctt
    14401 ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc
    14461 gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc ttcattaata
    14521 atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc ttctctgatt
    14581 tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccattt agaaccatcc
    14641 cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat tggagacgcg
    14701 attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt atctctagaa
    14761 gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc ttcattcgtt
    14821 gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt gtgccgtttt
    14881 tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg tgttaaagga
    14941 ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa atcgactcct
    15001 ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg ttcttccgga
    15061 acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt ttgtgcgact
    15121 tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt aacgagtaac
    15181 agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc gttctttttt
    15241 tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca acgttcttgc
    15301 ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc ttggaccact
    15361 cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac gtggtgttgc
    15421 ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct cctgtccgtt
    15481 aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga tgatgacctc
    15541 gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt cttcaaaata
    15601 ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt tatccgaaat
    15661 gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga tccattgatt
    15721 tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta tcatctgata
    15781 tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc tttgacgtcc
    15841 atacttgaag aagtatggat ctcttagata accgacattt ttaggataga tatgaatctc
    15901 ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag caattgggat
    15961 agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt cttcgaaaat
    16021 caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg caatcccgtc
    16081 tcctaatccg ccaaccgt
//
LOCUS       DACCSK010000062        15617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCSK010000062 DACCSK010000000
VERSION     DACCSK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.063"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            291..1430
                     /locus_tag="IVP39_001720"
     CDS             291..1430
                     /locus_tag="IVP39_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7586794.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="IVP39_001721"
     CDS             complement(1497..2072)
                     /locus_tag="IVP39_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7586795.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="IVP39_001722"
     CDS             complement(2077..3051)
                     /locus_tag="IVP39_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP7586796.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="IVP39_001723"
     CDS             3334..4302
                     /locus_tag="IVP39_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7586797.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="IVP39_001724"
     CDS             4415..4693
                     /locus_tag="IVP39_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7586798.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="IVP39_001725"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="IVP39_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7586799.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5159..5905
                     /locus_tag="IVP39_001726"
     CDS             5159..5905
                     /locus_tag="IVP39_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7586800.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="IVP39_001727"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="IVP39_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7586801.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="IVP39_001728"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="IVP39_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7586802.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="IVP39_001729"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="IVP39_001729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7586803.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="IVP39_001730"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="IVP39_001730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7586804.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="IVP39_001731"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="IVP39_001731"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7586805.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="IVP39_001732"
     CDS             10642..11184
                     /locus_tag="IVP39_001732"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7586806.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="IVP39_001733"
     CDS             11207..12763
                     /locus_tag="IVP39_001733"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7586807.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="IVP39_001734"
     CDS             12777..13679
                     /locus_tag="IVP39_001734"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7586808.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="IVP39_001735"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="IVP39_001735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7586809.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="IVP39_001736"
     CDS             15122..15544
                     /locus_tag="IVP39_001736"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7586810.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5170 a         2826 c         3384 g         4237 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaaga
//
LOCUS       DACCSK010000063        15423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCSK010000063 DACCSK010000000
VERSION     DACCSK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.064"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(12..482)
                     /locus_tag="IVP39_001737"
     CDS             complement(12..482)
                     /locus_tag="IVP39_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7586811.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IVP39_001738"
     CDS             complement(641..1099)
                     /locus_tag="IVP39_001738"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7586812.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IVP39_001739"
     CDS             complement(1270..2415)
                     /locus_tag="IVP39_001739"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7586813.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IVP39_001740"
     CDS             complement(2428..3345)
                     /locus_tag="IVP39_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7586814.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IVP39_001741"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IVP39_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7586815.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IVP39_001742"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IVP39_001742"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7586816.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IVP39_001743"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IVP39_001743"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7586817.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IVP39_001744"
     CDS             6871..7752
                     /locus_tag="IVP39_001744"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7586818.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IVP39_001745"
     CDS             7749..8465
                     /locus_tag="IVP39_001745"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7586819.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IVP39_001746"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IVP39_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7586820.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IVP39_001747"
     CDS             9182..10183
                     /locus_tag="IVP39_001747"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7586821.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IVP39_001748"
     CDS             10180..11541
                     /locus_tag="IVP39_001748"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7586822.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IVP39_001749"
     CDS             11501..12652
                     /locus_tag="IVP39_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7586823.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IVP39_001750"
     CDS             12627..13796
                     /locus_tag="IVP39_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7586824.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGVVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IVP39_001751"
     CDS             13971..15350
                     /locus_tag="IVP39_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7586825.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5036 a         2725 c         3288 g         4374 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca ggggttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaa
//
LOCUS       DACCSK010000064        15402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCSK010000064 DACCSK010000000
VERSION     DACCSK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.065"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..552)
                     /locus_tag="IVP39_001752"
     CDS             complement(<1..552)
                     /locus_tag="IVP39_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7586826.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICL"
     gene            complement(638..1597)
                     /locus_tag="IVP39_001753"
     CDS             complement(638..1597)
                     /locus_tag="IVP39_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7586827.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(1609..3132)
                     /locus_tag="IVP39_001754"
     CDS             complement(1609..3132)
                     /locus_tag="IVP39_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7586828.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(3142..3705)
                     /locus_tag="IVP39_001755"
     CDS             complement(3142..3705)
                     /locus_tag="IVP39_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7586829.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(3711..4574)
                     /locus_tag="IVP39_001756"
     CDS             complement(3711..4574)
                     /locus_tag="IVP39_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7586830.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(4588..5484)
                     /locus_tag="IVP39_001757"
     CDS             complement(4588..5484)
                     /locus_tag="IVP39_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7586831.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(5659..6930)
                     /locus_tag="IVP39_001758"
     CDS             complement(5659..6930)
                     /locus_tag="IVP39_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7586832.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(6920..8437)
                     /locus_tag="IVP39_001759"
     CDS             complement(6920..8437)
                     /locus_tag="IVP39_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7586833.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8635..9525
                     /locus_tag="IVP39_001760"
     CDS             8635..9525
                     /locus_tag="IVP39_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7586834.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(9598..10422)
                     /gene="nadE"
                     /locus_tag="IVP39_001761"
     CDS             complement(9598..10422)
                     /gene="nadE"
                     /locus_tag="IVP39_001761"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7586835.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(10434..11906)
                     /locus_tag="IVP39_001762"
     CDS             complement(10434..11906)
                     /locus_tag="IVP39_001762"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7586836.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(12543..12905)
                     /locus_tag="IVP39_001763"
     CDS             complement(12543..12905)
                     /locus_tag="IVP39_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7586837.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            13128..13880
                     /locus_tag="IVP39_001764"
     CDS             13128..13880
                     /locus_tag="IVP39_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7586838.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(14020..14943)
                     /locus_tag="IVP39_001765"
     CDS             complement(14020..14943)
                     /locus_tag="IVP39_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7586839.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(14958..15230)
                     /locus_tag="IVP39_001766"
     CDS             complement(14958..15230)
                     /locus_tag="IVP39_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586840.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         4748 a         3157 c         2487 g         5010 t
ORIGIN      
        1 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
       61 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
      121 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
      181 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
      241 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
      301 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
      361 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
      421 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
      481 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
      541 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
      601 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
      661 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
      721 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
      781 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
      841 ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
      901 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
      961 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
     1021 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
     1081 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
     1141 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
     1201 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
     1261 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
     1321 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
     1381 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
     1441 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
     1501 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
     1561 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
     1621 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
     1681 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
     1741 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
     1801 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
     1861 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
     1921 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
     1981 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
     2041 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
     2101 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
     2161 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
     2221 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
     2281 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
     2341 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
     2401 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
     2461 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
     2521 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
     2581 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
     2641 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
     2701 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
     2761 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
     2821 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
     2881 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
     2941 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
     3001 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
     3061 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
     3121 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
     3181 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
     3241 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
     3301 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
     3361 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
     3421 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
     3481 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
     3541 tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg tagcaggcac
     3601 gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga
     3661 aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc
     3721 attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt
     3781 aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc
     3841 cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc
     3901 aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga
     3961 gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca
     4021 atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag
     4081 ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca
     4141 agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc
     4201 agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac
     4261 cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta
     4321 tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta
     4381 tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa
     4441 gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc
     4501 gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag
     4561 ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta
     4621 cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga
     4681 tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt
     4741 ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat
     4801 cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt
     4861 aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga
     4921 gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca
     4981 aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat
     5041 cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat
     5101 agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg
     5161 acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat
     5221 aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc
     5281 tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa
     5341 gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa
     5401 taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt
     5461 ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac
     5521 aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata
     5581 acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat
     5641 cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt
     5701 catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc
     5761 gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg
     5821 tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc
     5881 caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca
     5941 atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc
     6001 atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt
     6061 agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat
     6121 ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc
     6181 tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc
     6241 aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa
     6301 catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt
     6361 actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg
     6421 ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg
     6481 tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc
     6541 tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc
     6601 tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt
     6661 tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat
     6721 ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat
     6781 ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc
     6841 agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa
     6901 agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac
     6961 atctgaccac attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt
     7021 ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg
     7081 tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt
     7141 gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt
     7201 catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt
     7261 ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa
     7321 atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa
     7381 taacatttac caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag
     7441 tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag
     7501 gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat
     7561 tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat
     7621 cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat
     7681 gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat
     7741 cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg
     7801 aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt
     7861 cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca
     7921 tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc
     7981 ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg
     8041 catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac
     8101 accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat
     8161 ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca
     8221 caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga
     8281 cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat
     8341 atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta
     8401 cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt taatttatac
     8461 atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac ttacttattt
     8521 tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat ttaatataac
     8581 ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat atatatgcaa
     8641 ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa aacaaggatc
     8701 aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc aaaagaactt
     8761 ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc aaacatcatc
     8821 aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa aatcgatatc
     8881 agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat acctattggt
     8941 cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca tgattttatc
     9001 gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc ccgcattctc
     9061 tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac agaggataca
     9121 ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc aaatgacaat
     9181 tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg gacgagccct
     9241 ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga taatcttaat
     9301 caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat gaatggagaa
     9361 cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg gcatttgcga
     9421 atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg taaaggattc
     9481 ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt ttcttttcta
     9541 ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc tttattgtta
     9601 tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg tcttgttcca
     9661 ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta gataatcatc
     9721 aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt tcccatcttc
     9781 aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg cttgaagcaa
     9841 ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc caccatcacc
     9901 aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta atacggctcc
     9961 tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt tgatatttcc
    10021 tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt gtgcatcaac
    10081 agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa gagcagcttt
    10141 tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa attgatagtt
    10201 ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac ggcctgctaa
    10261 agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg gatatttttt
    10321 caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat caattgttgg
    10381 tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc ctcctatgct
    10441 tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat tttatggttc
    10501 cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg tttatactct
    10561 tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag tgcaggtaat
    10621 tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc tgtaaagtct
    10681 cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc ttcttcttct
    10741 cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc aaaatttcga
    10801 gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt gcttgataac
    10861 ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt gaatactgca
    10921 ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca tacatcaatt
    10981 ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt cgaagcatag
    11041 atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt agatatatag
    11101 gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc taattctttc
    11161 gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc gactaagaac
    11221 acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt tccataagat
    11281 tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt cccagcacgt
    11341 acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca gactgctgca
    11401 tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc gatgacagat
    11461 ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt caacagtgct
    11521 gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg ttcatttgca
    11581 aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa attttgtaaa
    11641 tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat atcggaatca
    11701 ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa aaccgcgtat
    11761 ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa atcagctcga
    11821 cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg cagcgtcaaa
    11881 ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt tttcatttcc
    11941 tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt ctgaaaaagc
    12001 acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac tatcctttct
    12061 tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat ccgagacacg
    12121 aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt cctactacaa
    12181 tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct acaacataat
    12241 caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa
    12301 gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata
    12361 taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt
    12421 aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct
    12481 tgttttttat tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt
    12541 tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt
    12601 ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct
    12661 tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt
    12721 ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt
    12781 gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc
    12841 actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc
    12901 ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca
    12961 tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct
    13021 tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca
    13081 tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa
    13141 taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga
    13201 ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag
    13261 aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata
    13321 tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc
    13381 aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac
    13441 ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac
    13501 aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa
    13561 aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag
    13621 aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag
    13681 aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct
    13741 ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac
    13801 ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa
    13861 ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac
    13921 ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc
    13981 cattctttca caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata
    14041 aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca
    14101 agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc
    14161 gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac
    14221 catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg
    14281 ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa
    14341 aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc
    14401 caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat
    14461 tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa
    14521 aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt
    14581 aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc
    14641 tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat
    14701 gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat
    14761 gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc
    14821 tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat
    14881 tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc
    14941 cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga
    15001 ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc
    15061 caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc
    15121 ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca
    15181 taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct
    15241 ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc
    15301 caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac
    15361 attataatga tactgtaaat atatctttat tattagtgaa tg
//
LOCUS       DACCSK010000065        15354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCSK010000065 DACCSK010000000
VERSION     DACCSK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.066"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(85..1380)
                     /gene="purB"
                     /locus_tag="IVP39_001767"
     CDS             complement(85..1380)
                     /gene="purB"
                     /locus_tag="IVP39_001767"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7586841.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1393..2526)
                     /gene="purK"
                     /locus_tag="IVP39_001768"
     CDS             complement(1393..2526)
                     /gene="purK"
                     /locus_tag="IVP39_001768"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7586842.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2510..2998)
                     /gene="purE"
                     /locus_tag="IVP39_001769"
     CDS             complement(2510..2998)
                     /gene="purE"
                     /locus_tag="IVP39_001769"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7586843.1"
                     /translation="MAAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3414..4721)
                     /locus_tag="IVP39_001770"
     CDS             complement(3414..4721)
                     /locus_tag="IVP39_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7586844.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4724..5305)
                     /locus_tag="IVP39_001771"
     CDS             complement(4724..5305)
                     /locus_tag="IVP39_001771"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7586845.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5421..5518)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5877..7169)
                     /locus_tag="IVP39_001772"
     CDS             complement(5877..7169)
                     /locus_tag="IVP39_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7586846.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7166..7789)
                     /locus_tag="IVP39_001773"
     CDS             complement(7166..7789)
                     /locus_tag="IVP39_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586847.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7792..8628)
                     /locus_tag="IVP39_001774"
     CDS             complement(7792..8628)
                     /locus_tag="IVP39_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7586848.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8645..9532)
                     /locus_tag="IVP39_001775"
     CDS             complement(8645..9532)
                     /locus_tag="IVP39_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7586849.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9548..10831)
                     /locus_tag="IVP39_001776"
     CDS             complement(9548..10831)
                     /locus_tag="IVP39_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7586850.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11514..12197)
                     /locus_tag="IVP39_001777"
     CDS             complement(11514..12197)
                     /locus_tag="IVP39_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7586851.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12215..12832)
                     /locus_tag="IVP39_001778"
     CDS             complement(12215..12832)
                     /locus_tag="IVP39_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7586852.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12842..13666)
                     /locus_tag="IVP39_001779"
     CDS             complement(12842..13666)
                     /locus_tag="IVP39_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7586853.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13659..14219)
                     /locus_tag="IVP39_001780"
     CDS             complement(13659..14219)
                     /locus_tag="IVP39_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586854.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14392..15348
                     /locus_tag="IVP39_001781"
     CDS             14392..15348
                     /locus_tag="IVP39_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7586855.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4505 a         3426 c         2631 g         4792 t
ORIGIN      
        1 agaagcagaa ccgctaacgg atctgcttct tttttggtta tttttgctat tctattttaa
       61 ttgaattttg caactcactc ttctttattc caaacctact cgtttgaata tcacgtcaac
      121 gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt tcttctttag ataataccga
      181 tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc acttggttgt cccaagcgta
      241 cgcagttttt ggctgaacaa gatcataagc ttcttctctt gtcattccct tatcgatcaa
      301 tttcagcata acgcgctggc tataaatcaa accaaatgtt gcagacatat tccgcttcat
      361 gttttcagga aagaccgtta gatttttaac gatattgcca aaacgattta acatgtaatt
      421 caataaaatc gtggtatcag gcaagatgat tcgttctgct gaagaatgag aaatatctct
      481 ttcatgccat aatgtgacat tttcataggc agtgaccatg tgtccgcgca ccactctggc
      541 tagaccagcc atattttctg aaccgatcgg attccttttg tgcggcatag cagaagaacc
      601 tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt tcggattttt gcaatccgcg
      661 tatctctgtt gcaaaacgtt caatacttgt agcaataagc gccatcgctg agaaatattc
      721 agcatgtaga tcccgaggga gaacttgagt agagatttct tgtggacgga tccccaattg
      781 tttacataca tactcttcaa taaatggcgg gatatttgcg aacgttccca ctgctcctga
      841 gatttttcct gcttctactc cttttgccgc atgttcaaat cgttcgatgt tccgtttcat
      901 ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt ggttcagcat ggacaccgtg
      961 tgtccgaccc atcatcactg tatatttgtg ttcttttgct ttttcgccaa tgatttccgt
     1021 aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc ttgatgagat agccataagc
     1081 agtatctacg acatccgtac ttgttagacc ataatggacc cattttcttt cttctcccaa
     1141 tgtttcagaa accgctcgtg taaacgcaac gacatcatgg cgtgttaatt tttcgatttc
     1201 caagatccga tcgatatcaa atgatgcatg ctcacgaatt tttttcacat cttcttgcgg
     1261 gatttcacca agttctgccc aagcttcatc tgctaaaatc tcgacttcga gccacgcacg
     1321 gtatttgttc tcttccgtcc agatattccc catttcaggg cgtgtataac gttctaacat
     1381 gttttttctc ctctaatccc aaatattcgt ttgtttgatt tcttccagcg tttgctggat
     1441 gtcttctgtc aagatagtga tatgtcccat cttgcgacct ttttttgctt gtttttttcc
     1501 atagaaatgg aattgccagt ctggcttatt ctcaatcaag cgatagcttt ccagcagttg
     1561 ttctcccaaa acattgacca ttactgcatt gcttatcaaa cgtacttttt ctcctaatgg
     1621 ccaaccacaa atacctcgaa tatgggcatc gaactgactc atagaacaag cttcaataga
     1681 ataatgtcca gaattgtgag gacgaggagc taattcattc acatatagcc cgcctgtttt
     1741 tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc actgcttctg caatcacacg
     1801 ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc gcggctggag caatcgtttc
     1861 atgaagaata ttgttgtggt ggatgttttc cacgaccgga aaagtcgtga aatctccttg
     1921 accatttcct gccaccatga tcgaaatctc tttttcaaac gggatccatg cttctaaaac
     1981 acaggttcct tctcttaaaa ggtccatggc aggagctaga tccgcccgac ttttcaaaac
     2041 atactgcccc ttgccatcat agccgcctcg agtggttttc aaaacacaag gatagccgat
     2101 cccgtcaatc gcatcttgga tatccgtcgg actgacgatc gttgcataag gagcaatcac
     2161 gatgttattc gtttccaaaa aagatttttc taacaggcgg tcttgcgtaa tcgcaagcaa
     2221 gtctgtccct tgaggaataa atgacatggg taaaatcgca tttaacgcct ctacactgac
     2281 gttttcgaat tcataggtaa tgacatctgt tcttctggca agctctttca gcgcaaaggt
     2341 gtcatcatag acactttcaa tgtgccaatc tgcaacttgt gcggctggac aatcgctcac
     2401 aggatccagc acacctactt taaagcccat ttcttttgca ctaatcgcca tcatacgacc
     2461 taattgaccg ccaccgacga tcccgatcgt tgaaccaggc aacaaaggtt taaccaagtt
     2521 gatcactact ttctataaca gttttagcca acattgaacg tctttcagcc aatttagcct
     2581 ccaattctaa atcatacatg gaaagcatct gtatcgcaag tagtccagca ttaattgctc
     2641 ccgcttttcc gattgctgta gtagctacag gtactcctcc aggcatttgg acaatcgaaa
     2701 gaagcgaatc caaaccattt aatgttcgtg attggaccgg tacaccgata accggcaaag
     2761 ttgtttttga tgcgaccata cccggcagat gcgcggctcc ccctgctcct gcaataatga
     2821 ttttgattcc tttttttcga gcgttttgtg catattcaaa catcaaatct ggggttcgat
     2881 gagcagaaac gacctttttt tcataagaca caccgaattc atccaatatt tgacatgctt
     2941 ctttcattgt ttcccaatct gaagtactcc ccataatcac agagattttt gctgccattg
     3001 tttttcctcc cagtttttct acattcatat tttaacaatc actttttttg atgtcaaaga
     3061 aatttcgaat gttaacaaag aaaaacattt tattgttcgt gtttattaaa gataaaaaaa
     3121 aggaatgtga caaaagttag gtcacattcc tttttatgga taaacggtgt tctcctgcca
     3181 actcctcggt cacaaggaac aatattcaac aaacatgaga agcttttttt tgaaattgtt
     3241 ccttgtgact cggagatgaa cggcaggctc acgacctctt ccttcaacgg tgttcaatca
     3301 gctgaccctc ggtattagct caaacaaacg gcaaaatacg aggaactatt tttccgtttg
     3361 tttctctaac aatcaggtca aaacgctgat ttcacaacct ttcttttgct ctattacttc
     3421 ttgaaggctt cttccaatat ttcttcatct tttttcaatc cattaaagat caaattcaat
     3481 atgatagccg taatgctact cattacgatg ccatttccag tgaacatttg caatgtttgc
     3541 ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca gcccaaatcc aacagaaaca
     3601 gcaacaatca atagattttt atcattgctg aaatcaactt ccaacagcat acggattcct
     3661 tggactgcca ccatcccgaa catgactaac attcctccac caagtaccgg ctcagggatg
     3721 atttgtgcta acgcaccgat tttagggaat aatcctaaaa caatcaagaa gaatgcagag
     3781 aaatagatgg gttttcttgt tttgattccc gaaagttgaa ccaatccgac attttgagaa
     3841 aagccagtat aagggaatgt attgaaaata ccgcctaaga taacggcaag tccttctgca
     3901 cgatatccct ttttcaattc ttcttctcca acatttttcc cagtaatgtc accaagtgca
     3961 aaatatacac ctgttgattc taccatgctg accatggaaa tgatgatcat tagtaaaatc
     4021 gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt gtggaacatg gaacaacggc
     4081 gcatctccaa tcgcgctcag atcaatcatt cccatgaaag cagctaagat gcttcctccg
     4141 actaaaccaa tcaataccgc gattgaacga atgaaccctt ttgccagcat ttgcacgcct
     4201 acaattagga gaatcgtcac aaatgcaagt agcagatttg ttttgtcacc aaaatcagct
     4261 gctgttgcat tacctccacc cattttctcg attgctacag gaatcaaagt caaaccaata
     4321 acggtgataa ccgttcctgt tactatagga gggaatagtt ttttaatttt agaaaagaca
     4381 ccagaaacta acacaacaaa gatcccagaa gcgataatag atccgtagat agctcctact
     4441 ccttcattgg ttccgatcaa gatcagtgga gctactgctt ggatcgcaca tcctaaaacg
     4501 acaggcaagc caattccgaa aaatcggtta actgtcaatt gcaataatgt cgctaatcca
     4561 cacataaaga tatcaattga aatcaaataa gtcatctgtt cttgattaaa gcctagtcct
     4621 gtaccgatca aaagaggcac cgctactgct cctgcataca tcgctagcag atgctgcagc
     4681 cctaaaaccg cagcttttcc attttgtgtt tctttttcca cgattatgcg tcctcctcaa
     4741 gaaactcaac ttgtccattc gtcaaggaag caatacgcgc aagagaaacg actctcaatc
     4801 ccatttcttc aagtaactga cgtccatctt ggaaagactt ttcgatgacg atcccgattc
     4861 cttctacttg cgctccagct tgctggcata attcgatcaa tccttttgcc gcttgtccgt
     4921 ttgccaagaa atcgtcgata atcaatactt tgtcttctga agataaaaat ttacgagaaa
     4981 tagaaatcgt acttgtcact tgtttcgtaa aagaataaac agaagctgtc aataattctt
     5041 catccatcgt caaacttttt gcttttctag cgaagatcat tggaacatct aattctcttg
     5101 cagcaaataa agctggtgcg atcccagaag cttcaatggt cacaactttc gtgattcctt
     5161 gttctttaaa tacttctgca aaccgttttc ccatttgttc catcaataca gggtctactt
     5221 ggtgagtgac gaagctgtcg acttttaata cgccctcgcc taatatacgt ccgtcttttt
     5281 gaatacgttc aactaattct ttcactttat ttgctccttt atcatttatt aataccgaaa
     5341 taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa gaaaaacacc ctccacttta
     5401 tcataagaaa agaagggtga aatagccaat cttttactta tagtccagca tttacggtac
     5461 tggctagaaa ctgtcatcca tattatgacg atatataagt aggtatgtaa aactaataat
     5521 agtgtaaatt cattttcttg caactgtttt gcacgtgaag gcagtttgcc atatttgaat
     5581 aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta ctttctgaaa ctgacaacgt
     5641 ggtttactta tttttttgta aaatctaatg atttttaatg cattattcct ttttgttcgg
     5701 gtttaaatag ttatttttat tttctcctat ctaaaaaagc agcccacata aaaagaaggc
     5761 atgacaaaaa gcatgtcata gccccttttt ccgaataaat agtgttcaaa agacagccta
     5821 tcggcaataa ggcaagattt tataaactac gggaagtaat ttccgtaaat ctattcttat
     5881 tcttttaagc tgacaagctc gtcaatcact actgcttttc ctgaagcaat agaatgattt
     5941 gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt gagttgctgc ccgattccac
     6001 ggatcttctt ctttttcttc accaaataga tcatctaata aacgcggatc tcctccgcca
     6061 tgtccgcccg cttcttgttc gacgattacc tcatatggct caccgaacat cggatagaca
     6121 tggatggact gaagaacggc tgctccttca tcctttttat ctccacctgc attgatgtaa
     6181 gacttttcga tcactttcat ttcgatccgt cctttgctgc cattgaaaga aacattgaat
     6241 ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa tcgcaccagt ttcatatttg
     6301 accgttacgc ctagtgtatc ttcaatagag atatcatcgg aaaatacact gcggtcacga
     6361 atatatccac tgtctttttc tgcttctaaa tacatttctc gcaattcctc attggctgac
     6421 aggtctatag caaatggatc agctttagct agttcgcttc catagcatcg actgtaaaat
     6481 atttctactc cgcgtttctc cgcattatgc atcccataaa agtttaatcc accgaatgca
     6541 aatacctttt ggggacggct gttcaaccaa aaattcacta gatcaaaatg atgggtagat
     6601 ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat gccatcttct gaaataatct
     6661 gccccgtgtt gcgtatttaa cagccattcg aagtggatag aaaacacatc accaatcaca
     6721 tcactttcta tcagttcctt tatcttcgtg tgatacggtg cataacgata attaaaagtg
     6781 actcgcaggc ttcttcccgt ctgtttttgg accgttatta tttcttgagc tttttcctca
     6841 tcgatagtca gaggtttttc gcaaataaca tcatatcctt ttttcattgc acgaatgata
     6901 taatcatgat gcgttcgatc tactgacgta ataatgactt cagccggccg tacgtcatcc
     6961 agtagttgct caaattgtga tggcatacat gtctttactt ttttctgtcc atacttgttc
     7021 acaagtatcc gatttgcata atccatcctt gtttgagata catcacaaaa accgactagt
     7081 tcacaagttt gatgatacgt agaggcaatt gcctcataaa aaaaacgcgc tctcccacca
     7141 agtccgactt gtacatattt tttcttcatt taaagtcatt ctccttttct tttaagcaaa
     7201 atactgtgac aaatggcagc cctggataca gtaatgcagt gagtaaaaaa tcatgctgta
     7261 tacaccagaa cagtccatac aaaaggcata aataccacag aaaccccttg acgttttttg
     7321 acaacagcca gatattcctt ttgatcaaat aaacaagact atctgcttta tcttctttgc
     7381 agatatcaat tagcgtacta aaaaaactat gggatacaaa aagcaacaga cacaatagaa
     7441 aaggaatcag ataccaagct ccaatctttc ccactaataa ataccaatca actgcaaaaa
     7501 taatgacaaa cagctgaaac aacgtaatga tgaaacacat ccgtttgatt tttcctttcc
     7561 tccaacatac ctttttttct gccaagtaat ggacagatag ataataagaa ggatacatca
     7621 tcaaaaaata aatcagatag acaacacgag cagacagcac taatcctaaa gtgaacaaac
     7681 atcctagaac taaagcgaaa agagcattac tgataagact ggttaaaaaa atccgacaga
     7741 catagtagtt gagcttgtaa agaaacatct cttctattgg ctttttcata ttcagccctt
     7801 cagaccgctt gtactgatcc cctggacaag atatttattg aagaataaga aaatcagaaa
     7861 aaccggtagc agcgacaacg tagacatcgc aaacatactc gcgtaagtcg tactagatcc
     7921 agcgtctgca aataatttga tggcaatcga taccgtgtaa gatctcggct tgtttaaata
     7981 aatcaatggt cccatgaagt cgtcccattt ccaataaaac tggatgatga tcgtcgtgat
     8041 gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag acactatact tgctgcatcc
     8101 gtcaataatc gctgattcat ctaactcttt ggggatgctg accatgaatt gcatgatctg
     8161 gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc agcggcacat acgtcccaac
     8221 caaattcagc cgattgtaaa taataaactg cggaatcaaa atcacttgtg caggaatcat
     8281 catcgttact aacatgacga cgaaccagaa tctttttcct ttgaaatcga ttctcgataa
     8341 tgcgtaagca atacaagtcg agctcagaac tgtccctatc gtcgaaactg cagagatgac
     8401 aaacgaattc ctaaagaaag taaaaaatga aattccagca aaccccgccc atccttttgc
     8461 gtaattttcc cattgaaaag tctctggaat caaattcagc gaaccactca taatctcttg
     8521 atccgtttta aaagaacctg cgatcatcca taagaccgga taaatcatga ctagacctaa
     8581 tagcaaaata aaaccgtatt gaaaccattt cgttactgta ttattcattt cttcctcctc
     8641 tcattcacac aacgacccat ttttttgatg atttgaaaat cagaaaggtg acagctccca
     8701 taatcaacag catgatccac gaaatagcac tagcataccc catagaatta aaagaaaaag
     8761 cttggttata cacataaaga gcataaaagt tcgtggcatc attcggacca ccttgggtaa
     8821 tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt ctgcataacc aagttataca
     8881 aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa cgtctgccat ttgttcgctc
     8941 catcgatctc tgccgcttca taatacgagc ctgggatctc tttgatacct gcagcaaaga
     9001 tgatcattgc cgaaccgaat tgccaagcag acatcaaaac aagagggatc atgaccagtt
     9061 tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg ttccaggaaa atattcacta
     9121 acccgttttt agaaaacaac tcccgccaaa ctagagcgat agaaatactg ccaccgatca
     9181 aactaggcac ataatacaaa gaacgaaaga aagtcgttcc tttgatattc ctcgttaaaa
     9241 gataagcaat caatagacca aagatcagtt tcacaggtac agatgcaaat acatagagaa
     9301 aagtgactcg gatggagttc aaaaaacggg catcactcag catctttata taattatcca
     9361 agccgatcca tgagactgga tttcccaaac gataatcggt gaacgaataa taaagtgaca
     9421 agatcatcgg tatcactgtt atcaatagaa atcctaggat gaatggagaa gcaaatacgt
     9481 aaccgaccac attgtcttga tccaagaacc gttttagttt ttcttttttc atgccttcct
     9541 cctgctgcta tcctagaatg tttgacgcaa actcataaag actctctgct gcttcttctg
     9601 ctgttacttc ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg
     9661 gattcaaagc actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta
     9721 aataatcaaa tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat
     9781 tcgacatgat tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt
     9841 gaaagaaatc aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca
     9901 gcatttgcga agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat
     9961 taatcaactg aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac
    10021 ttgatagaaa caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg
    10081 tacttgcagc tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca
    10141 gctggaaaaa atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt
    10201 cccgattata aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta
    10261 taaaatcttc gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga
    10321 tttgttcaaa ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa
    10381 atatattttt attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt
    10441 gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc
    10501 cttgctccac atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt
    10561 tcagttggaa aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat
    10621 atccttcata atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa
    10681 gctcgattac ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca
    10741 gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta
    10801 aagctaagat gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt
    10861 ttcaatatac tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat
    10921 aaaaaccaag acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa
    10981 aattagatag tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga
    11041 ggataattac gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta
    11101 aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc
    11161 tgtatgaaat taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat
    11221 tggtcagttc ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt
    11281 gcctttttta gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca
    11341 tttttttgtt agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag
    11401 gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca
    11461 aaagacgagg aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca
    11521 tgattctttt tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta
    11581 cttggtggcg tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct
    11641 tccgttggac cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt
    11701 actgcttctg cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac
    11761 ttttcatcta tttctgtata tttcgacgtt accgtactga taactggaat atcaactact
    11821 cgacaaactt cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa
    11881 ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca
    11941 cctacaccgg agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga
    12001 tgaggtgtaa aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca
    12061 atatccgtag taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct
    12121 tctaaaatga tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc
    12181 gtaaattgtt ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt
    12241 gatacgtctc gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg
    12301 catcttgcaa tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca
    12361 gcgcaggatt ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt
    12421 cccatgaatc atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt
    12481 cctttgtttc caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat
    12541 tgtatttgct agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa
    12601 tgatactagg aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt
    12661 caataaattg ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag
    12721 acagattctt caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg
    12781 ctggccaacg gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt
    12841 cctacttttc atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat
    12901 actcagcgtt ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta
    12961 tatcttcgtg tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga
    13021 tccatttagc tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt
    13081 tgtaccagtt ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg
    13141 gctccgtaat cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac
    13201 tcatcacatc ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag
    13261 ctgagaccat cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt
    13321 ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact
    13381 catcaatttc tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg
    13441 ttttaacatc tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc
    13501 tgctggttgt ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga
    13561 ctgcttgatt gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg
    13621 ctgcgatcat aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt
    13681 cgttgttctt cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc
    13741 acccaatcga gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga
    13801 cgaaaattca aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca
    13861 aggtatctac cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg
    13921 ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg
    13981 tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca
    14041 taatcagtga aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca
    14101 tctttccatt tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt
    14161 tcagtttcaa actgttctaa taatacactc ataataagat caatatatac ttgatgcacg
    14221 tttatcccct ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg
    14281 ctgctttcat ttttttaatg tttcaagtcc tctcctcttt attcttcgtg atttttgtta
    14341 taataataga cagacaagaa actcaatcca actatgaagg agcgttttca gatgaaaaaa
    14401 tatcaatggg gaatcgttgg tcttggtacc atcgcacatg agtttgccga aagcttcaat
    14461 caagagacga gcgaactaac agcagttgct tccagaactt cagaaaaagc ggaaaacttc
    14521 gctcaccgtt acaatattcc aaaagcttac ggaagctatc aagaaatgtt agacgatgct
    14581 gaaatcgaca tcgtgtacat tgctgtacca aatagacagc atatcgacca tatcttggca
    14641 gcattggaag caggcaaaca cgttctatgt gaaaaagcca tcacgatgaa taaaaaagaa
    14701 ctggctgacg ctatgcgctt agcagaagaa aagaatttga tcttagcaga agcgatgaca
    14761 atcttcaaca tgccacttta tcagcagtta cgctcaataa tggatacagg aaaactgggg
    14821 gctttaaaaa tgatccaagc acctttcggc agttacaaag aaccagaccc taaaaaccgg
    14881 ttcttcaatc cagaattagc tggaggagct ctgttggata tcggtacgta tgctgtatct
    14941 ttcgctcgtt tctttttgag ctctcaacct gaagtagttg cctctacaat ggttcctttt
    15001 gaaacaggcg tggacgaaca atccgtcact atcttgcgta acaaagaaaa tgaacttgct
    15061 gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg gtatcgttgc ctttgagaac
    15121 ggttatgtga cgattgctga ttacccgcgt gctgatcgtg cggagatctt atttacagat
    15181 ggtacaaagg aattcatcga aagcgggaat acatctgaag caatgaatta cgaaataaag
    15241 aatatggtac acatgattga aggtgacttg cccaatcgct ctctcttctt gacaaaagat
    15301 gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa atatgtaatt ttaa
//
LOCUS       DACCSK010000066        15317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCSK010000066 DACCSK010000000
VERSION     DACCSK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.067"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IVP39_001782"
     CDS             complement(150..1337)
                     /locus_tag="IVP39_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7586856.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(1430..2749)
                     /locus_tag="IVP39_001783"
     CDS             complement(1430..2749)
                     /locus_tag="IVP39_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7586857.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKMITSI"
     gene            complement(2746..3399)
                     /locus_tag="IVP39_001784"
     CDS             complement(2746..3399)
                     /locus_tag="IVP39_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586858.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            3687..4620
                     /locus_tag="IVP39_001785"
                     /pseudo
     CDS             3687..4620
                     /locus_tag="IVP39_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4528..5244)
                     /locus_tag="IVP39_001786"
     CDS             complement(4528..5244)
                     /locus_tag="IVP39_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7586859.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(5294..5503)
                     /locus_tag="IVP39_001787"
     CDS             complement(5294..5503)
                     /locus_tag="IVP39_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586860.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            5780..7053
                     /locus_tag="IVP39_001788"
                     /pseudo
     CDS             5780..7053
                     /locus_tag="IVP39_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7160..8191)
                     /locus_tag="IVP39_001789"
     CDS             complement(7160..8191)
                     /locus_tag="IVP39_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586861.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8198..9028)
                     /locus_tag="IVP39_001790"
     CDS             complement(8198..9028)
                     /locus_tag="IVP39_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586862.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(9025..9831)
                     /locus_tag="IVP39_001791"
     CDS             complement(9025..9831)
                     /locus_tag="IVP39_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7586863.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(9837..10661)
                     /locus_tag="IVP39_001792"
     CDS             complement(9837..10661)
                     /locus_tag="IVP39_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP7586864.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(10651..11751)
                     /locus_tag="IVP39_001793"
     CDS             complement(10651..11751)
                     /locus_tag="IVP39_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7586865.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            11929..12714
                     /locus_tag="IVP39_001794"
     CDS             11929..12714
                     /locus_tag="IVP39_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP7586866.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            12747..13130
                     /locus_tag="IVP39_001795"
     CDS             12747..13130
                     /locus_tag="IVP39_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7586867.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            13206..13337
                     /locus_tag="IVP39_001796"
     CDS             13206..13337
                     /locus_tag="IVP39_001796"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7586868.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            13306..13542
                     /locus_tag="IVP39_001797"
     CDS             13306..13542
                     /locus_tag="IVP39_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7586869.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(13620..14591)
                     /locus_tag="IVP39_001798"
     CDS             complement(13620..14591)
                     /locus_tag="IVP39_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP7586870.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4624 a         3339 c         2672 g         4682 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcatttt aaagataaaa aattcttcat cctgcaatac tttatattct atattgtaat
     1501 tattcagaat attacttaca atatataaac ctaatccatt actattttcc ttatttaaat
     1561 caaatttaac atcaaatatt ttatccatat tcgaaatttt attgttaccg tatgaattct
     1621 ctatatataa ccaatcatta actatcccaa tattaattac cccatttaca tcagtatatt
     1681 ttaccgcatt actaatcaaa ttagatagaa taatctttaa agctgttttt cctatataaa
     1741 cattctcgtc taatatataa ttattgacag ttattttctt ttgatttgct aatatttcat
     1801 acttttttaa tatgtcttct aatgtatcat ttattttcaa atattcttca tcattattca
     1861 aattttctat agaatgaact gataatattt gagaaatatt ttttgttaaa ctatcaacga
     1921 tttcaataca ttcattaata taaatatctc tttctttata ttttccaata ttgtatttca
     1981 tgttttccag tattatttta agactagcaa gaggggtttt aagttcatgg gatgcacctt
     2041 taaaaaagtc atattttaac ttttctaatt ttaaaatttc tttattttta aattccaaat
     2101 cgtcaattgt tcttaataaa gtagaataca aatcattaat ttgttgtttt aattctccaa
     2161 cttcatcatt agagctaact tttaaactca tttttttatc aagtttcatc attttatcag
     2221 taacaatttt tatttcttgt atattatttt ttattgattt tgcataaatt aaagaaataa
     2281 tagcagaaaa taaaattgat attaataaag aatatggtaa aaatttaagg cttaaatctt
     2341 tcgcatcttt ttgcatatca gctgtagaaa caaattgtag atttgttttt ttaccatcat
     2401 taagctttat ttctctttct tcaattatca aagaattact atcactttct aaattaacat
     2461 ttatattatc tttgatttgt atttcattct tattattttt ttcttttatg aatactttta
     2521 tttcactact cttggaataa aactccaaag tttgttctat atattttatt tcttttccat
     2581 tcatattact agaaatttca tttgccatat tatgaatctt ttcttttctc gtctccaaat
     2641 atgttttagg aaagataaaa aaaattaatg aatgaactaa tataattatt attccaagaa
     2701 cagaaaatat ttgtatgaac atttttggaa atatctttaa ttttttcatt ttctctccaa
     2761 tttatatcct acatttctta tagtagttat acagtctaat tgtaattttt ttctaagttc
     2821 ttttatatat acatctatta ctcgatcata aggagtttct tcgctatctt tccatacata
     2881 atcaataatt tgcattcttg ttaacacttg tccattatta tccaataaac attttaatac
     2941 ttctagttct tttgcattta catctatttc ttcaccattt attttagctg tatagctatt
     3001 aaaattaacc gatagatcct tatattcaaa cttctctaaa tgtccataat tcttcttaat
     3061 taaagaatct attctagctt ttaagaccgg caatgaaaat ggcttttcta catatccgtc
     3121 tactaaatta gtaaatgcat caattttata ttcttcatca ctaaatgcag tcaagattag
     3181 aattggtaaa ttgcttttct ttctaatttc tttcaacact tctaaaccat ttataaaagg
     3241 tatctgaatg tctaagataa ccaaatttat atcgctatta aattttgaca aggcttctct
     3301 tccatcttta gcttgaataa cagtatatcc aaattctgaa agatattcac ttattccctc
     3361 tcttatcata ttatcatctt caacagttaa tattttcata tatacctcca cttaagccta
     3421 tacactagat attatacagc atattatggc tctataatat ctagtgttgg tgacgccttc
     3481 tgttgccaat actttttctt ttttttaata aatgcaaaca gacaccttga aatcctttag
     3541 aaaaaaggct tctataatat tttgtgttgt tggaaaatcg aaggcgattt tctactaaca
     3601 ctttttcttc gtttcaactc aaaaagagac tcacttttgg taaaatttag tcgaccaaaa
     3661 catcacccta ccaaaggagt cgctctatgg ataatcatac tagaaaatta ctcggattaa
     3721 cagataaaca tttatttttt gatgaagaat ggctgattga aaaagaatat aaaggcgttc
     3781 aagcgcagtt tattaaaggg agattggatg actcaccttc ccattgtgaa cactgtggat
     3841 cgattcgtat tatccgcaac ggttcctata cgacacgtac tcagatgttg aaagtcaaag
     3901 aaaagttgac tattttagaa ttaaaacgta cccgttttct atgctatgat tgtgggcaga
     3961 ccttttctgc gaaaacggac ttagttgatg aacatcatca actaacaaag gaactgaagc
     4021 aggcgattct catagaactg tacgaaaatc aatcacgtaa actaatcgct aagaagtact
     4081 tcgtctcgga tgggacggtg acacggatct tgcgtgaagc aacgaagcat taccagccac
     4141 gtatgaactt ccttccgacg gtgttgtgta tggatgagtt caaatcaatg aagtctgttt
     4201 ctagttccat gagttttatt tgtgttgatg ggacaaccca tcagctattt acgatccttg
     4261 aagatcgcag actttacaaa ctaactcaat acttcctgca ctttcctaga aaagcccgtt
     4321 taaaggtcaa gtacctagaa tgccagctat tgtcagctgc ttaaaacggt ctttccaaat
     4381 gcggagatcg taactgatcg ctttcatatt gtccaacata tcaatcgcag cttcaatcaa
     4441 ttgcgtatcc agatcatgaa tcgttttcga gcctcgcact ctgaaaatca gaagaagtac
     4501 cgtcggctca agcgatattg gaaactctta ctcaaagatt ccacgacatt ggagccgctc
     4561 aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat cgtcgatgaa
     4621 ccgttgtgca caatggtgtc ctgaacgtac agcgacacct tccatatcca gagcagtcgc
     4681 cacatcacgg ggatggatgc catccaaatt aaaagcaacc acgccagtat gatgttcagg
     4741 atcttgcggt ccgtaaatgg tgataccgcc gatcgcttgt aatttaggta aaacgtaggc
     4801 aaccagttct tgctcataac gatgaatatt ctccatacca atctcattca agtaatcgat
     4861 tgctcctgca atgttcggag taccagcttc aaatttccat ggcagctctt tccatgaact
     4921 atcgtaaaga ttgacaaaat ctatcatttc accgccgaat tcgactggct ccatttgttc
     4981 taacaattga cgtttgccat ataatacacc gattcctgtc ggcccgcaca ttttatgccc
     5041 gctaaaggcg aaaaaatctg catccaacgc ttgtacatca actgtcatat gcggtgctgc
     5101 ttgggcaccg actggactat cctcaactac aacagcctgt tcaaccctca gccgtttcaa
     5161 gcacaacaaa taaccttctg gatctggttt gccgacctga agatcatcga tcgtcacaaa
     5221 ggaatcaatt ttttccttaa tcatcggatg tgcttcaaaa aaatagtcta gctcccgcag
     5281 ttcagaattg agactactcc gattttccag tgctgctgat ccgctgctca aaatgttcag
     5341 catccactgg tctcccaacc gcttgactta tcccttcata aaaggacagt tctgctgccg
     5401 tcccaacacc ttgttgatag tctgcaaaag gaattttata gccgaactgc tccagtaaat
     5461 aggcttcagt aaatagtgat gaatactctc gtaaaacatc cattcactgt caatcatcgt
     5521 cccatcaaaa tcaaaaataa gtgctttggt catcttcttt cccccttacc caaaacaagc
     5581 aaaagctgtg tcctgctctc tctacagtat cagcataaaa actgccgtct gaaaagtcaa
     5641 aggtgaaaga aatgtggcgc tataatttct aggactgata gtaaatcatt ctatcagtcc
     5701 ttttgagcat aaaaaaacaa aacatacaga agctccgtta gaattgaatc accacaacac
     5761 aatcaaagga gcgttctgta tgcctgatct tcatcttatc aaactattac taggaatcaa
     5821 agatggaaac attcaaatca ctcatgtcga gaagaaaaaa atacattcaa tcaacagtat
     5881 cgttgtttct gccacgctca ttaaaacgat tcagccctgt cctcagtgta aacaggtgaa
     5941 tcaacatggg ctgatcgtta agaacggaaa gaaaaaatcg ctggtccttc tcaataagtg
     6001 tgcgaatcaa cggacttatc ttgctttggc aaagcaacgg tacctctgtc gtggctgtca
     6061 cacctatttt acggcgaata cctacattgt tgaaaaaaac tgttttatcg ccaaacaagt
     6121 acattataaa atattggagg aattggctga aaaacaagca atgacgacga tttctaaaca
     6181 ttgcggcgtt tcttggtcaa ccgtttcgcg aacactcgct tctctgctcc ccatgacaaa
     6241 cgtgaaaata aattggttgc ctcgctgttc attggtagat gaatttcgtt cattgaaaaa
     6301 tcaagtcggt ccctattctt tttcttgtat ggatggcgat actgggaaat tactggatat
     6361 tcttccttca cgtaagaaga aagatctcat ttcttatttt atgcaatttg aacgacgtgc
     6421 acgtttaaac gtaaaaatac tcgtaacaga tatgaacgcc tcttatgctt tgctgataaa
     6481 agagtgcttt cctcatgcca aactcgttgt ggatcgcttt cacatcgtca agcacctcat
     6541 tcgcagtttt aaggatattt gactatgagt catgaaaagc ttcgaccgaa acgatccaat
     6601 acaagccaaa cactatcgac aagtgaaggc tttatctcga cttctgatca aacgacagga
     6661 cacgcttgtt tatgataaat ggacaaaatg gcgtaatttt ggttgggcct atttaaccga
     6721 aagtgaagtc gtagaacgtc tactttctac ttctgatgaa ctacgaatag cttatgctta
     6781 ctatcaagaa attcttcaag ctttttatga caaagaagcg gacatcttct ttcagcttgt
     6841 aaaaacaatg cccaagtcgg ttccaagaga actgcatcat ataaaaaaag cgtttataaa
     6901 ctatgaatcc ggcattcgtt tagccttaga gttgccttac tcaaatggca aaatagaaaa
     6961 tctgcatacc cacatcaaag cattgaaacg aatagcttac gggttaggat gagaaccaga
     7021 attttcttaa tcaatcatct gatcacttat taaacataaa aaaacatcta aagcagaaaa
     7081 tcaattctac cttagatgtt cttatatcac tctatcagtc ctatttgaca aagagccaga
     7141 aatgtttgcc gactatacac tagctttgtt gtgcaaaatg ctgagtatca atttccacag
     7201 ggattgctag aatatctttc agctgaatgt ataaggcatc gctaattttc tcttcccgat
     7261 cgttcaaccg atccactaaa atttctttgc cctccagcgt ttgaaaaaca taccgttgaa
     7321 tgttgcctaa aataaatgat tccttccata gaaccggaat cttaaccccc tgaggaaccg
     7381 gttcaaattg aatcagctcc ggtcttaaat aataaatgca gcgattgccc ttagcggaaa
     7441 actcgtgggt atactgattc agctcatacc attcgaaacg attatagttg ccgataaatt
     7501 ccgcaacaaa tcggctctga ggattctgat acaattcctt cgaggtggaa acctgagcca
     7561 cccgcccagc ttccatcaca aaaatccgat cactgattcg catcgcctct tcttggtcat
     7621 gagtcacaaa aatcatcgtc atccctagcg attgctggat agcgcgcaaa tcccgctgca
     7681 gctgttttct aattcgggca tccaaagcac ttaatggctc atccagcagc aggactttcg
     7741 gttcagtgac caatgagcga gccagtgcca cccgttgtcg ttgaccgcca gaaagaaact
     7801 ggacattttt atctgcctgc tccgtcatat ccaccaatga tagcatctca aaaacctttt
     7861 cctgaatcac ctcaggactc agcttcttta ccttcaaacc aaaggctaca ttttcaaaaa
     7921 cagtcatagt tggaaacaat gcatactgtt gaaaaacaaa gccgatattg cgatccttcg
     7981 taggcttatc ggcaatattc tcattgtcca gcacaatctc accgctcttt ggttgttcca
     8041 atcccgtgat acagcgcagc aacgtagact ttccgcaccc gctgggacct agtaaagtaa
     8101 ccagctcacc cttttcaatc tgaatttcat caatagctaa aatctgtttt tcatcaaaaa
     8161 acagattgag atcatgtatt tctaaaaaca ttttccgtca caactccttt aagggcttct
     8221 ttttcgactg cttctcttcc ctcgtctgct taagggacag ctgttgcttt tgtcgataag
     8281 tcagcataaa ggtagttaaa gcaattccca ccataaacag caaatacaaa accatcacag
     8341 cgctagccaa atggccgttt tcgttcatcc gccgcagcat atagatgcgt accgtctgaa
     8401 atcgaccgcc aatcaacagg ttcgtcagca tgtactcccc aaaaaccgaa gaaaaaatca
     8461 tcagagaaac tagggtcaca ccgatacgga tgtttggaaa cagcacctgg ataataatcg
     8521 tagacatcgg ggcgcccaag gtttctgctg catccactaa ttccttcaga ttaattaagc
     8581 gcatctggtt gttaagacta agatagacaa tcggcaacgc cgtaataaac aatgcaccta
     8641 ccaacactac aaacattggg ataccagttt cagcatacgt tcgaagcaaa gccgttacca
     8701 aaatcactcc tggtaaagca tacggcagca ggagcagctt ttccagccaa cgattcaaac
     8761 gagggaaata caaatgaatc caaataatgg tgggaatcat caacaacaga atcgtcacca
     8821 agaccacacc tgctaataaa agtgagcgac ccaccgcagc aataaattcc ctgtccgtca
     8881 gcagctgttg gaaccattgc aaggtaaaat cactaggtaa aagcgatttt gaccatttag
     8941 tggagctggc atacaaaatc gttccaataa tcggcagcaa gtaatacagg acaaagcaga
     9001 acaaaatcgg ttttattaac tttttcacgt ctgcaccacc ttgcctttct tttgaataat
     9061 caacattgcc gttaccagca ggacacctaa agcgactgcc aacgcactgc ctaaattagg
     9121 cttggcgaac acatctcccg acactaaagc cgcaattcga atcgttaaaa gatttacatt
     9181 actgctggtc agggcatacg ctgtttcata ggtccccatg ccattagcaa ataagatgac
     9241 aaaggtgctg gcaatcgatg gtcgcaaaaa aggcaaagcc accttgaacc aagcatacgc
     9301 tgatgaagcg cccagcaatt gtgcggactc cagccattcc tttttcactt ccttcatgct
     9361 gggatacaaa aacaagaccc ctaacggaat ttggaaatag gtatacgtga tggtcaatcc
     9421 ccaccaagaa tacaaattga agtccgctag aaatggcaaa atcattttca gcacacctac
     9481 attccccaac aaaataataa aagagaatgc cagcggaatc ccagcaaaat tagctgccaa
     9541 attggaaaca aaggtgattt tctcctgcaa tttagtcgat agattcatta agcataaaca
     9601 taaaataaac gtcccgatta acccaatcac actggagctg acagcaatca gtacgctgtt
     9661 aacaaaggcc tgtgtgtaat atttgttgct gaaaatatca ctgtaattgg ccaatgtcca
     9721 ctggttagtt aaatcactgt gcaagctgca cagaaccata aaaagcagcg gcagcagtaa
     9781 aaatgcacag aagataaagg aggcaggaac cagaaccaag cgttttttca tctctttcat
     9841 cccctcatca attcttgaat gtcctgcatt ttctctgacg gtgcaattcc taataaccag
     9901 caaaacaatg gagccgccag cagctgtggt acagtttgtt ccttcagctt ttggcgttta
     9961 tttgaaatca aataaaaagg aacttcacga tgtgcagcca agctgccgcc atgcagtccg
    10021 tgcgcatcca tcccgtgatc cgcagtcacc acgacttgat aacctttttg cagccattca
    10081 ggaaggtaaa tagccaataa tctgtccgcc tgattaaccg ctgctgcata ttcatgagaa
    10141 tcggcggtaa atttgtgacc gacatcatca atattcatcg aatgtacgac caaaaagtct
    10201 ggctgatact gattcactaa ataatgggca tccgcaaata aatgcgaatc agggtaagtg
    10261 tcctcactat aaaagatgcc atgctgaatc agtcgctcct cctgatgctg gatgcgatca
    10321 accaaacgat tatagggggc ttgattgtac aactcgctgt accagtggta agcggcggct
    10381 cctgtttttc cgccagcttc cttaactaat tcaaacagcg accactcttt ggatcgctga
    10441 gcatggtaat tactatagat gccattttca taggctggaa cgccggtcat cagtacttcg
    10501 tataacggac gtgaattgct gggcagctca ctttttacct tcaataagct tgcttgctgg
    10561 tgctcaacta aatgattcat gaacccaagc tgggcgacgg ctgtgtcgta gcgacagccg
    10621 tctagtataa ctacaatcag tttgtcaagt ctactttgca taggtcagta cctccgcctg
    10681 ccaaagctct ggaagctcta cagaggtctt gttccaagct tcattgtctt caactggcac
    10741 cgccgcttca taagcatcct ccggcagtaa tttttcagct acttctttag gcagctctac
    10801 cttgcgaata ggtcgcgcat agccctttgc caaattgatt tgcccttcat cagataaaat
    10861 atattcccgt gctagctttg ccgcattggg atgaggggca tttttattga ttaaggttgt
    10921 atacccgcta atgatggagc cgtctgttgg cacagttacc tcaaaacgct cggagtcaat
    10981 ttggtcacga taatttagtc cattgaagtc ccaaacaact gccacatcga tttcaccttt
    11041 ttcaagattt gctacgctgg catcaacggt ggacaaacgg cctttttctg ccattttttt
    11101 gtagtaattc agacctggct gaatatcctt ttcgtcacct ccgttggcaa tcgccgcagc
    11161 caatacagca aattgtgctt ggttggcaat cgtcacatcc cccacgctaa cttgatagtc
    11221 accctcttgc agctccttcc agctagtcgg tgcttgcttc acattctctt tatcggtgat
    11281 aaaggaaatg gttcccgtat agcttaacaa ccaatcccca tcatcatcct ttgcccaatc
    11341 cggaatttcc tcccaatagt tggtcttata aggtaaggtt aactctttgc tttcagccaa
    11401 cggaccgaag ctgataccga catcaccaat atcggcggtt ccattttttc cttctgattc
    11461 aaacttagcc aattcttctg cactggacat atccgtatcc acgtgactta agccgtattc
    11521 tttttcaagg tcgttccaag tgcctaccca atttgcccaa gtatccggca ttcccaagct
    11581 ggcaatctct ccttcttcct tggctttttc tataatcgta tctaaagagt cgtctgccgc
    11641 agccgattgc ttcgttgctt cacctgagcc gcagcctgtc agcaggatac ttactgttgc
    11701 tgcacaaaat aaaccgatag tcatgttacg aattttttca ttcattttca ttcattcttc
    11761 tcccctttcg ataattccat tctaacaaag ctttgtaatg agagaatcag tttcttgaaa
    11821 aggtttcatg aacaatgtaa aggttgtata tggtttgtaa atttcgacga attcccgtta
    11881 accttctaaa gcttttgcgg tatgatagta gaaaaaagga ggctccctat gcagtattat
    11941 gatcagcatc tccacacgta tttttccttt gattcagagg aaaaatttga aaactattta
    12001 gcctatcagc cggagttttt cgtttctacg gatcactttg atttaaacaa tccatctacc
    12061 ggaaaagatg acattccgga ttatccagcc tatataaaca aactggactc tttaaaagaa
    12121 acctattcca ccaaatttct gcgaggtatc gagattggcg ttgttccagg acaagaagag
    12181 cggatcgtgg attacctcgc tcagcatcct tatgatttaa agctggtaag tattcatcaa
    12241 aatggccgct ttgattatat ggacgatgtg gttttaacca aagatcccat tcaaactacc
    12301 aaggaatatt ttgatcagat gttgcaggtc ttgacggatt ttccagaagg agatattttg
    12361 actcactttg attatggatt acgccgcttc accttcacag cggaagaatt cgcggagcat
    12421 tttgaggctc agctgctaac gattttcaaa aaggttgttg aacgagaatt ggcgatggag
    12481 ttaaatgcca agagcttctt gaaatacggc aacgaagacc tttaccgtta tgccgttcct
    12541 ttgtatcaaa gtgtcggcgg aaagctcttt accttaggct cggatgctca tgttgccgaa
    12601 gattatcagc tgggctttaa aaaaatgagt cagctgttgg cagactgtca ggtagaaaag
    12661 ttgctagtca ttcaaggaaa agaacgccgc ctgacgccgc tgccgatttt gtgattccta
    12721 tataatagaa gaaagtggtg aaaaaaatga gcaacctgtg ggactacaat caagaggcac
    12781 ccatccatta tttaattgcg cgacattggg atgcgttgaa aattgaggcg gtttgccgat
    12841 ccttgttagc agccgttcca aaacaacaac tggaaaattt tttggtggct gacagcttgc
    12901 agcgggaaaa agttcaggct tattttgcag cctttaaaga tcagcctttg gaatatttgc
    12961 atgcgcagtt tcatcttttt tatcaagtag ccgccccaga tgactacaac gatttacgag
    13021 gacagctgca attgaccttt caagcggatg aaaccgccta taccgtttta ttgggaatgg
    13081 ctcgccttgg tgatcaggcg aaggtcgagt ggcggatttt cgatatttga tggcaggatc
    13141 aaggcgtttc agttgcaaac cttcctctct ttcaattaga aaaaccgcag caggaagaag
    13201 tttgtgtgct ggtcaccgat gctggtgaaa ttcgtctccg cttatttcct gaccaagctc
    13261 ccctggctgt agccaactgg cgctccctag ctgagcaagg cgtttatgac aacacacctt
    13321 tcggctcgtg tcattaagga atttgttatt caaggtggcg ctttggatgg ttctggtgat
    13381 gaagctgttt ccagctatga gggctttttc gctgatgaag tgcatcgcgg actttatcat
    13441 tttaatggcg ccttggcctt aggggaccac ggcccccaca ccaacggcaa ccagtttttt
    13501 attgtgcaaa acacaaaggc gcaggctgac ctgctaatgt aaagcaagca tacttggagc
    13561 ttggaggact accggagctg gatggtcgtt atactgtttt tggacaggta taccaagggt
    13621 tagaaatcgt agcagcgatc gcagctcagc cgacaaacag tgaagatgta ccgattgagc
    13681 cagtgttcat tcgacaaatt aacttcgaaa aaacatcgta aaaagacact cactgacttc
    13741 agcaaaagct ctattcttga gctggctgca gctagtgagt gtctttcatt tatccataag
    13801 ataaagcttg gattcaataa ataaataaat gttttggtta aagctgtgac cagcaaatga
    13861 ggaaccgtgc gagccagttg atctgccaat gcaaacattt ctttaggtga aggcgctggc
    13921 gtgtctgcca acggatactc cagtcccaat gcttggtaac cgtgcatacc atagcagtgg
    13981 tacggtagta attctaacgg tacgaaccga tcaagatctg ccacaaaaca agcaatcgtc
    14041 agcagctcgg tcaggttcat attgatacct ggaaccacag gaactcgaat tcgcacttcg
    14101 cagttaggat actcaattaa aaagcgacga atattcttca aaatttgttg gttatctaag
    14161 ccggtccatt gtttgtggag cttgctggta aacattttta catccacaaa caatgtatcc
    14221 acatagggag ctaccttttg gatttcatta taagcaccac aaaagctggt ttccagtacg
    14281 gtattgattc caatgtattt cgatttttgc aaaatttctt tagcaaaatc cgcctgaacc
    14341 agtgcctcgc ctccgctaat ggtcactccg ccatccgagt gaaagtaaaa aacagcatcc
    14401 ttctcaatct catccattac ctcttcagcg gtcatggtct ggccaaatcc tttttcaatc
    14461 tgcttgcttt gtgattcggg aatcgaacac caggcacagc gtaatgggca gccctttaaa
    14521 taaacaacgg tccgtaaacc agcaccatca tgtagagaac cctgttctat tcttaatact
    14581 gttccttcca tatagatttt tgaattacac actagtttaa actaaaacac acaaggaaaa
    14641 acttaccatt taatcataaa attagctcat ataaatatac taggatgtaa aatagactgg
    14701 tcaaaatctg gtcaaaaaag aggactaata caggtagtcc tcttttttgt tattttaata
    14761 ttctaaatca ttttttattg gtttattccc ttattcgatg tagactcaaa tcactatatc
    14821 actagatatc aaacggcgtc atacggaatc aaaaacatca aatttgacct gttaaatggt
    14881 cattgaataa cattcaaaat caataaaacg gagtcagaca cggagtcaaa aacgctgaga
    14941 gtaaaaaata tatacagcct tgtaggtaaa gtaccacagg gctgtatatt ttttatttca
    15001 gattacctta aatgattcca tttacttttt atctcatttt ttcctactag ttagtatcaa
    15061 atactctcct attttctcaa taaactattt ttggatacat ggtatctgaa acagctaaaa
    15121 ccttgtcatt acagcaaaaa atcacgttat ccagactgta gacaaagtcc attttatgga
    15181 tgatgtctac agtcttttct tttataatag atttatgaaa acgaatgcaa ggagtgatag
    15241 ctatgatgtc taaaaacaat acgaatggta gaaatcaatt tgcgatgctt acgattgatg
    15301 atctagtttc tcaagat
//
LOCUS       DACCSK010000067        15032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCSK010000067 DACCSK010000000
VERSION     DACCSK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.068"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..301)
                     /locus_tag="IVP39_001799"
     CDS             complement(<1..301)
                     /locus_tag="IVP39_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7586871.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKA"
     gene            complement(301..4338)
                     /locus_tag="IVP39_001800"
     CDS             complement(301..4338)
                     /locus_tag="IVP39_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7586872.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(4351..4809)
                     /locus_tag="IVP39_001801"
     CDS             complement(4351..4809)
                     /locus_tag="IVP39_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7586873.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(5107..5625)
                     /locus_tag="IVP39_001802"
     CDS             complement(5107..5625)
                     /locus_tag="IVP39_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7586874.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(5627..6244)
                     /locus_tag="IVP39_001803"
     CDS             complement(5627..6244)
                     /locus_tag="IVP39_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7586875.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(6241..7095)
                     /locus_tag="IVP39_001804"
     CDS             complement(6241..7095)
                     /locus_tag="IVP39_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7586876.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(7092..8003)
                     /locus_tag="IVP39_001805"
     CDS             complement(7092..8003)
                     /locus_tag="IVP39_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7586877.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(8044..9378)
                     /locus_tag="IVP39_001806"
     CDS             complement(8044..9378)
                     /locus_tag="IVP39_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7586878.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            9586..10416
                     /locus_tag="IVP39_001807"
     CDS             9586..10416
                     /locus_tag="IVP39_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7586879.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(10660..10909)
                     /locus_tag="IVP39_001808"
                     /pseudo
     CDS             complement(10660..10909)
                     /locus_tag="IVP39_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11100..11294)
                     /locus_tag="IVP39_001809"
     CDS             complement(11100..11294)
                     /locus_tag="IVP39_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586880.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(11799..11984)
                     /locus_tag="IVP39_001810"
     CDS             complement(11799..11984)
                     /locus_tag="IVP39_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586881.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(12834..13175)
                     /locus_tag="IVP39_001811"
     CDS             complement(12834..13175)
                     /locus_tag="IVP39_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7586882.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(13175..13405)
                     /locus_tag="IVP39_001812"
     CDS             complement(13175..13405)
                     /locus_tag="IVP39_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7586883.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            13692..14030
                     /locus_tag="IVP39_001813"
     CDS             13692..14030
                     /locus_tag="IVP39_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586884.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(14235..14474)
                     /locus_tag="IVP39_001814"
     CDS             complement(14235..14474)
                     /locus_tag="IVP39_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7586885.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(14450..14797)
                     /locus_tag="IVP39_001815"
     CDS             complement(14450..14797)
                     /locus_tag="IVP39_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7586886.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         4515 a         3128 c         2544 g         4845 t
ORIGIN      
        1 cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc gctccattta
       61 gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag atagccaagc
      121 catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc atccgtttat
      181 gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca tgtggaagaa
      241 ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac agctttttca
      301 ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg ctcctgcaag
      361 cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc ctgtagcagg
      421 caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat ttgtctccaa
      481 aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt ttggataata
      541 cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat cattttctat
      601 gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt ttctatagct
      661 gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg aagcattgaa
      721 tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat actctcctgc
      781 actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat tcatactgtc
      841 aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta cttgtagtac
      901 accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc
      961 ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat cagatagccg
     1021 atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca gatgcagagg
     1081 ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat agaagtatcc
     1141 gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt atgctcgata
     1201 cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat ctaatgtatg
     1261 gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat tgtaagcagt
     1321 gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt gccaattttc
     1381 ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc gcaagatcca
     1441 tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa tgattttgta
     1501 ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg gctctccgat
     1561 tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat ctactgaata
     1621 gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc ctttcgctac
     1681 tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact ctgcgccatt
     1741 gattggcata ttactgcctt caattagttt attgatattc atgtggattg gcttgaattc
     1801 atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta tgttaggctc
     1861 ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat ctttaccttc
     1921 cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt gacctaacat
     1981 ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat ctactaactg
     2041 ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg ctccagtttc
     2101 atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact
     2161 tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg caaattcgac
     2221 tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac ggcctttatg
     2281 ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat cattctcata
     2341 atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc cgtcttgagc
     2401 tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac ctaaagaaat
     2461 cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag tcgccactac
     2521 ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt ggtacttcat
     2581 gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc cagctccaaa
     2641 tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg ttctcttgaa
     2701 ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg cattgtaata
     2761 tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat ttcgtccaat
     2821 agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa tggagaagat
     2881 acttggcggg ttctcttgat acttattttt cagccaatga tgataagcaa ctgtgttgat
     2941 cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag aagaaccatc
     3001 accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac gatagtgtac
     3061 atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat gagtaaaccc
     3121 tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt cattatcttc
     3181 agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc tcgtacgata
     3241 acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa cgattaggat
     3301 tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat agccattttt
     3361 aagccctagt gtaagcggag ttcctccgat tggtgtacga tttattgctg tattctcact
     3421 tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga tttgctcatg
     3481 gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa tatccagact
     3541 cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg cagcattttt
     3601 agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat ctagaacaaa
     3661 aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta gatagacatt
     3721 gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg gcagaacagg
     3781 ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt caaaattccg
     3841 ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt cgcctgcagt
     3901 tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt ttgtataatc
     3961 actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt gatagcgttc
     4021 attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa acttcgggta
     4081 gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg ggtcttgcgt
     4141 agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa tgcgctggag
     4201 tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt cttcgctacg
     4261 ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt ggttatctat
     4321 tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg ggatcttttc
     4381 tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc cgacatacca
     4441 ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat aggaggatag
     4501 acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat cgattgctaa
     4561 acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc attcaagggt
     4621 ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta agaatacctc
     4681 atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat gatagactga
     4741 ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct tcaattttaa
     4801 ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa caatcaacaa
     4861 aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa tgatatcctt
     4921 tttagttaaa aaacaccaat tatacaacat ttattattaa aattaaatta taaaatttta
     4981 taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca actacgcttt
     5041 cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt tattcgtaca
     5101 attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa tagctcctcc
     5161 taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga tcaaacaagc
     5221 aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag ctactgataa
     5281 aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac ttgtgtaaag
     5341 gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt tgataaaaag
     5401 caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat attctctagt
     5461 atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac cattagagat
     5521 attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt gcttggcgaa
     5581 cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat tcctcctttt
     5641 taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa aacaagaaac
     5701 tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca agaaggatca
     5761 acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag aaaaatgcag
     5821 taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta aagagaagcg
     5881 cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt cccccacccc
     5941 aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt gctaaactat
     6001 cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt gaaccgaact
     6061 ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga cttgcagttc
     6121 ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg ataagacgtg
     6181 gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc gatcgattca
     6241 tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt gtgcaaaaaa
     6301 gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat aattccattg
     6361 tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt tttcttgatc
     6421 ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg aattgatcgc
     6481 aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa tatacaaatg
     6541 attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata agtaaaattg
     6601 acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca ggggcaaata
     6661 agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg ttacaaaccc
     6721 cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag gaaaatcaag
     6781 cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca ctactagcac
     6841 agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa tcgtatccag
     6901 atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa cgatttcttc
     6961 cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa tgattcctat
     7021 tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac tggtcttttt
     7081 gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg tgcaagcttc
     7141 aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat agccatcgca
     7201 taacccatat caaaagctac gaacgccttg ttccacatat gcagattata aaaaagcaaa
     7261 gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg aaaaatttgg
     7321 aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat taatggcaaa
     7381 gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga ttcatataaa
     7441 gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc agaactccat
     7501 attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca agctggtcca
     7561 gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga aagcagctgc
     7621 atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa agctgtacgg
     7681 aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac actgaaaatc
     7741 gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga tttccaaaat
     7801 aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt catcttggat
     7861 gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc tagcataaat
     7921 accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc ttcttttttc
     7981 tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac agtcaaaaat
     8041 ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg ccaatccttc
     8101 ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta atgtcgtgta
     8161 atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt cgtccgccat
     8221 ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat tctcattggc
     8281 tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta cttctggaga
     8341 agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt atggtacttc
     8401 caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt ctgggagctg
     8461 catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt aataattgat
     8521 attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac cagacccaaa
     8581 ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt cttggatcca
     8641 ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt tgtcaaacca
     8701 actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc ataatggata
     8761 aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg ctgtttcttc
     8821 taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt taaataactt
     8881 tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag gatcaccttc
     8941 atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt tttctacata
     9001 tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg
     9061 tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag tccagatgtc
     9121 accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat tgaaatcctc
     9181 aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc agaaatcaat
     9241 attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac tggcatcgct
     9301 tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca gcaaccctgt
     9361 aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc cggttttaat
     9421 ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct aaaaaaatta
     9481 ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt ttaatctttc
     9541 gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt tagacaccag
     9601 aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc gaaatatctg
     9661 caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta cctgttccaa
     9721 ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat tgcaacagaa
     9781 gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga gggtgcgcct
     9841 gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat tatctctgga
     9901 atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga cagcgtgggc
     9961 aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga ctttagagct
    10021 aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga aatgatcgat
    10081 gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat
    10141 ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt agtcccaagt
    10201 actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa cttctcagag
    10261 ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg cgaaggcgaa
    10321 tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga accagtaaca
    10381 ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag aaacacccgt
    10441 tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat aaccaccctt
    10501 caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc ctaaagacgc
    10561 tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc acctcttaaa
    10621 aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta cactcagctt
    10681 atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt
    10741 ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc caaatttata
    10801 ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat tgattataaa
    10861 ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc tactttgtac
    10921 gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg tatttcaatc
    10981 gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg cttcactgac
    11041 agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg caatctattt
    11101 tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa taaagccgta
    11161 taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat cccgataaca
    11221 ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata gaatagaact
    11281 agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa attcacggca
    11341 ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt tccctacaaa
    11401 ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa tctaaattcc
    11461 atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta tacataacct
    11521 tgatacttcg accacttata tatcgaacac acaaatacct atccattgca ggagtactac
    11581 ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt actcttagta
    11641 aagtatctga actattaatt tttaaactat catttttatc ctcaactaca tgcacatctg
    11701 ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga attgcaatat
    11761 cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat tataaaataa
    11821 ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt aataaatgtt
    11881 gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat ataccctata
    11941 aaagctaata agatggttag gaatccttta gaatttttac tcattcatag acacttcctt
    12001 aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag tgactcaaaa
    12061 taaaaaaaac cttacattat actggaatga actgtattga tagtgtacct aaaagctcta
    12121 ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga tgtaatcaat
    12181 tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct ttgagattct
    12241 actttggttc actatctgac ccaattgcac attttcgatc attacaagta ttggtgtcgt
    12301 tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa tttctgtatg
    12361 gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat gatatgcctt
    12421 tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga tcccaaattt
    12481 tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa gagtttagat
    12541 tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt attgtcgcga
    12601 cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa
    12661 tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt tagtttaaga
    12721 tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact tggccttttt
    12781 ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa tgcttaaaac
    12841 atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt ttctattttt
    12901 ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac ttgtcctttt
    12961 atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt
    13021 ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac gactagtcct
    13081 ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca tacaatatca
    13141 ccttttttag gaatataaat tctttcttct gacactataa aatttctctc ccttcaggag
    13201 ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca ctaaatggat
    13261 cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg atatattctt
    13321 ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca gaacttcctt
    13381 gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta tactatataa
    13441 cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt gtttaaagta
    13501 tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct ttaactaaaa
    13561 aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac tatagctaac
    13621 cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca aagacaacag
    13681 gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc tctgacaaat
    13741 ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt cggatcaaag
    13801 aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa gactttgcta
    13861 ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat gatccaactc
    13921 atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat acaactgcca
    13981 accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa tgataaacat
    14041 cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga agctatacat
    14101 tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc atttcttctg
    14161 ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc tctttctttg
    14221 aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg agtaatgatt
    14281 tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt caagacaagc
    14341 ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat tatgattgat
    14401 tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac tattttttcc
    14461 ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa acacaacgat
    14521 tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca cgggcattaa
    14581 tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc ggagtgagga
    14641 tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact tctccagctg
    14701 ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa ttctgaagga
    14761 gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc aatctttttt
    14821 tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt tttgtagaac
    14881 taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctc
    14941 ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt gttccttgtg
    15001 actcggagtg aaacggcagg cttacgacct ct
//
LOCUS       DACCSK010000068        14861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCSK010000068 DACCSK010000000
VERSION     DACCSK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.069"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(5..823)
                     /gene="purR"
                     /locus_tag="IVP39_001816"
     CDS             complement(5..823)
                     /gene="purR"
                     /locus_tag="IVP39_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7586887.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(990..1814)
                     /locus_tag="IVP39_001817"
     CDS             complement(990..1814)
                     /locus_tag="IVP39_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7586888.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(1787..2467)
                     /locus_tag="IVP39_001818"
     CDS             complement(1787..2467)
                     /locus_tag="IVP39_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7586889.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(2490..3428)
                     /locus_tag="IVP39_001819"
     CDS             complement(2490..3428)
                     /locus_tag="IVP39_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7586890.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(3673..4524)
                     /locus_tag="IVP39_001820"
     CDS             complement(3673..4524)
                     /locus_tag="IVP39_001820"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7586891.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(4678..7251)
                     /gene="mprF"
                     /locus_tag="IVP39_001821"
     CDS             complement(4678..7251)
                     /gene="mprF"
                     /locus_tag="IVP39_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7586892.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7392..8198
                     /gene="proC"
                     /locus_tag="IVP39_001822"
     CDS             7392..8198
                     /gene="proC"
                     /locus_tag="IVP39_001822"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7586893.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(8277..9833)
                     /gene="rny"
                     /locus_tag="IVP39_001823"
     CDS             complement(8277..9833)
                     /gene="rny"
                     /locus_tag="IVP39_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7586894.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10244..11293)
                     /gene="recA"
                     /locus_tag="IVP39_001824"
     CDS             complement(10244..11293)
                     /gene="recA"
                     /locus_tag="IVP39_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7586895.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(11402..12643)
                     /locus_tag="IVP39_001825"
     CDS             complement(11402..12643)
                     /locus_tag="IVP39_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7586896.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(12883..13461)
                     /gene="pgsA"
                     /locus_tag="IVP39_001826"
     CDS             complement(12883..13461)
                     /gene="pgsA"
                     /locus_tag="IVP39_001826"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7586897.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(13670..14554)
                     /locus_tag="IVP39_001827"
     CDS             complement(13670..14554)
                     /locus_tag="IVP39_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7586898.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         4260 a         3300 c         2588 g         4713 t
ORIGIN      
        1 cctcttattc tgaaaaataa tttcctggca caaccgtaat atttttcgtt tgagtatcta
       61 catctttgac ataaagcagt gacgtataat catcgatggc gcgatgaccg ttgaattttg
      121 attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa ttcttcaatc aagctcttca
      181 ttccgttgac tgttccgccg cctttcatga aatcatccac tacaaggaca cgtgaaccac
      241 gtttcaaact tctcttagac agttccatct tttcaattct ttcagaagat ccagacacat
      301 agttgacact tactgttgat ccttctgtta tttttgagtc ccggcgtaca ataacgaatg
      361 gcgcattcag ataataggac acagcttggg caataggtac gccttttgtg gctacagtca
      421 ttacagcatc gattttttca ccaaggtatt tagaagcaat gattcttcca acttgacgca
      481 gcagttcagg ttctcctaac aaatcagaga gataaacata tcctcctgga agcagacggt
      541 cttgttctga tagacgttct gctagagatt caatatattt tttcgcctct tcatatgaaa
      601 tttcagggat gaacaataca ccacctgcag cacccgggat tgtttccaag gttccatacc
      661 ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc actgatagaa gatttagcag
      721 attcataacg ttcggcaaag cttgttaagg aaatcaattc atgcgggtga ttcaataaat
      781 actgtgtcat gtcaactaga cgttcactgc gacgtacttt cacctattcc aactcctttt
      841 aagaaatttt atcgttttac attatataag tttttcttca aaaattcgag cattttatga
      901 aaaaataaga aaatagttcg gtatttatta aaataaaaag aagcctataa aaaatttttc
      961 gtttttataa ggcttctttt ctttataatt tagttatttt ttcttctgaa ggcaaccaat
     1021 attcgtttga tgatatttac aatcaggaac aaagcaataa agactaatgt gattgttgct
     1081 cctggaggtg tatctagata aaacgagcta gttagtcctg acagcatccc tagaaatcct
     1141 acacaaacac tgatagcaat cacgacattg aagcttttgc ctaatcgcat accgattgct
     1201 gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg caatcgggat catcacagca
     1261 atagccacac cagtaatcac attgaacatc atcgacatca aatgaacggg tagcccatcc
     1321 acgtgggcgg tatcttcatc aaaagtcaga acatacatcg gccgtttgaa taaaaagaac
     1381 aatataccga tggcaacgaa taagattcct aaaaagatga cttgctccca tgtaatggtc
     1441 acgatcgaac caaacagata ggattggata cttgttgctg aattccctcc gctcaaattc
     1501 atcaatacta acgctagcgc caaaccacca gccattaaaa tggcaatgga gatttctgaa
     1561 taggttcggt agatcgttcg cagatactcc aatatcacag ctgctaatac aacgactagc
     1621 atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta acgctacccc tgctaaagag
     1681 acatgagaaa gcgtatcagc catcagcgat tgtcttctga tcactaaaaa cacacctagc
     1741 atcggggcga ttccggcaat gaagatagcg gcaagaaacg ctcttctcat gaattcatat
     1801 gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg gatctgtctg tccgcatact
     1861 gtttgatatc ttcatgatca tgggtgatca ttagaactga tttcccatgg acatgcgcac
     1921 tatgtcggag aagacgataa aattcatttc tggattcttc atccatacct gttgttggtt
     1981 catcaagaac aaataagtca gggtcagtag caaaaatacg cgctaaactg attcgctgct
     2041 tttgtccgcc agacaattca cctattcgcc gatttcgcat atcccacatc cctacagctt
     2101 ctaaagcacg ctgtacatgt tgatgatctt tttcagagag acgcttgaac cagcggcctc
     2161 ttggatatct tcctgaacgg actaattcga tcaccgtact aggaaaacct gcattgaaag
     2221 atgcaacttg ttgaggaata taaccgatac ttaatttttt tccttcttga tttttctcag
     2281 caatctttat cattcctttt gaaggtttca gtaatcctaa cgatgctttc accaaggttg
     2341 acttggctgc tccattttct ccagttaaaa tgacaaattc acccgcatct acatgataag
     2401 agacatcttc tagtacaggt tcatcatcat agtaaaaggt caaatcagat atttcgatat
     2461 aacgcacctt acaacctcca ttctaaaact taattgatac tttcttttaa agctttcaga
     2521 tttttttcca tgacagaaat ataattttcg cctgctttga tttgttcatt agtcaagctt
     2581 tctaaaggat tcaacacttc tagcttcact cctgtttcat tcgacaatgt ctcagctacc
     2641 ttcgaagagg cattttcttc aaaataaatc actttgatgt tgtttttctt cacaaattct
     2701 tttagctcag ctaagcgact tggtgtgggt tcctgatctg gtgaaacgcc cgaaatcgct
     2761 acttgtttta agtcgtattg attcgctaaa taagcaaaag cagcatgctg agtgacaaat
     2821 gtgcggtttg ttgcatcttt tagctcatcg gagtattttt gatccagtgc ttcaagcttt
     2881 ttgatataag catcggcatt cttcgtaaaa gtttcctttt tatctggata tttttcgcct
     2941 aattcttcag caatcgttct gacttctttt atagctaata ccggatcgag ccaaacatgt
     3001 ggatcgtatt catgtgaatg accatgccct tcttctgatt cttcatgttc gtctgcttct
     3061 tgcgattcta aacggtcgat tcctttagct gcttcgatca tcaaggtctg tttgctatcc
     3121 actgaatcct ttaacgaatc cacaaaaaat tccatgtctg aactattata cacgaaaaca
     3181 tctgcatcgg aaatttctgc tctttctttt gcacttggtt cgaaatcatg cggctccgtt
     3241 ccagcaggga tcaaaagttt cacgtttcct tcatccccta cgatctcttt tgtaaaatca
     3301 tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt ttttgtctgc ttccttgttc
     3361 gtatttccac atgctgcaaa cagtaaagca cctatcatta ccgtagctgt caaaagatat
     3421 tttttcattt atatgctcct ttcaatgaac aaatcggaat tttacgattt attaacgtga
     3481 actaatgtat caaaataaaa aaacacagtc aatagaaatc catcaatttc tgatttaagc
     3541 atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct agccatttca
     3601 ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat cacctatttt
     3661 acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa aaccttttaa
     3721 cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa cggttggtcc
     3781 gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt tgatttgatg
     3841 gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt ttttgatcat
     3901 ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt gatgttggat
     3961 ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc ttggtttgac
     4021 caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc ctttgccacc
     4081 gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt ccaaaccgat
     4141 ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc ctcttagagc
     4201 agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat tcttcttaac
     4261 aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag cagcttgata
     4321 aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa tgattttgtc
     4381 ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca tcaccatttc
     4441 caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat ttatcttggc
     4501 tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag tcgcaagtat
     4561 tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga ttttgtcatc
     4621 gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta gtgattctta
     4681 taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt ttcctatcag
     4741 actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag caaaataacg
     4801 tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga aggaatagaa
     4861 aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag aaaaatctgt
     4921 ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc ccattttctc
     4981 gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt ctttaggtaa
     5041 gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc
     5101 aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat atgatttatc
     5161 aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt gtgaaacttc
     5221 atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat ggtatccttc
     5281 tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt ctgttgctag
     5341 atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat gaagcatcac
     5401 tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat ctgcttgttt
     5461 cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat ctcctaaaac
     5521 aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt cgtcttcttg
     5581 gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg tgcctccgta
     5641 tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct tcgtagtatc
     5701 ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca aaccaataag
     5761 ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc
     5821 gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca aaaagccgaa
     5881 caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc gataaaattc
     5941 ttttctggat aaccacacag ctaacagaat caagacatat accaatatca actgccaaga
     6001 taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa
     6061 tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa agccaaccaa
     6121 tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt gaaatagccg
     6181 actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc ccgcaaaata
     6241 gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc
     6301 taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa
     6361 aggtagaaca taataaaaca accgataata taatagccaa actactgcta catcttgccg
     6421 aacaggcaat tgactcattc ctaagatcat caaaacgtca aaagtcccca ttccacctgg
     6481 gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga tcgaaagcat
     6541 cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg ctcccgtcca
     6601 ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact cgaagaacaa
     6661 tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac taccgccgaa
     6721 aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga catcaataaa
     6781 tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt ttgatacagt
     6841 ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca gacttgctcc
     6901 taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg cagcaagaag
     6961 aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga tcgtcaccca
     7021 atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga gcaggatcgt
     7081 catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc cattagcaat
     7141 atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca gttttaaaat
     7201 cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca cattttccca
     7261 ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt ttaatttttc
     7321 cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct aatgctaaag
     7381 gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa gctattattg
     7441 atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc agtggacatt
     7501 ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg
     7561 aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc attgaaaaag
     7621 tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt gcttcaggaa
     7681 aatcattaac agatttagaa acagcgcttg aagatcctca ggcctccatc gtacgtgtga
     7741 tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg
     7801 ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc agcgtctatc
     7861 ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca cccgcattcg
     7921 tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg tccaaacaga
     7981 cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg
     8041 ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca actgttgcag
     8101 gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga atcaatacga
     8161 cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt tttttcgtgc
     8221 cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat tttttattat
     8281 ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg tccaggataa
     8341 tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc gtctaaatcc
     8401 gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac tgcgtaactt
     8461 gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg acggatgtag
     8521 ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc tgctgcagct
     8581 acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc gatcgcgttg
     8641 attactactg ggttttcttt gtacttggca gctaattccg caccgatctc aacgtgagat
     8701 ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc acgttttgct
     8761 aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc aacctcgacc
     8821 gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc gatgatcttg
     8881 attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc cccgtattcg
     8941 cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat acgcgctgga
     9001 tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt
     9061 ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa atcgatacca
     9121 gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg acctttcatt
     9181 tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag
     9241 cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc tttggcacgc
     9301 tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc agtcgatttc
     9361 atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag ttctttttgt
     9421 tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg ctgttttgaa
     9481 ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta
     9541 cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa ttctaactta
     9601 ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc cttagcttct
     9661 aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat gattccagca
     9721 gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac cataaaccct
     9781 aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac cataaaatcc
     9841 acctccatac tatctttttc atttttatca tctatacagc ttctatgata aattattcta
     9901 aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa ctttattcta
     9961 atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc tacttattca
    10021 ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc tttaaattga
    10081 gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt tatgtaccca
    10141 ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca ggtcaaggag
    10201 agtacctact cttcagacca gccgtttttg tttatcataa tttttattct tctactaaag
    10261 gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca ataccataag
    10321 ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc atatagattt
    10381 tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa gcaccgcttt
    10441 tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt gagatcccta
    10501 aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg ttttttacga
    10561 ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt tctgcacgac
    10621 gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg
    10681 gattcccaaa catcacgcct actttttcac ggatttggtt gatgaaaata gcgattgttt
    10741 ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa cgtgcttgta
    10801 acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact aaagcagcaa
    10861 ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca gcgatctcta
    10921 gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc acacctaatt
    10981 tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc gttccgccat
    11041 ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc
    11101 catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg acatctaacg
    11161 caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc aatttcataa
    11221 tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag gctgctttac
    11281 gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac atttcattat
    11341 ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt
    11401 atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc gcattatatc
    11461 tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg tctttagatg
    11521 aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt ccagccactc
    11581 cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg gccatctgct
    11641 ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca atctccagaa
    11701 aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact tcagatacac
    11761 cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc gctgctgtta
    11821 cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa ttatcatcgc
    11881 cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat gctaaaagag
    11941 cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc acttcatttg
    12001 gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct tttagctcag
    12061 tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt agtttttctt
    12121 cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt ggtttcaact
    12181 cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa aaagtcccta
    12241 aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg gactgttgca
    12301 gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat tttttgtatc
    12361 cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct tttgtcaggt
    12421 catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt gtttcagcta
    12481 gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga taataaactt
    12541 cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc acgacttgtc
    12601 ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc cgctccctta
    12661 tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg agctgtgaca
    12721 aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc
    12781 ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc cttgtgactc
    12841 agagttagac ggcaggctca caacctctcc actaaacgat acttacattg atcctttgaa
    12901 cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga tcagacaagc
    12961 ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat tattgataaa
    13021 cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc aagctgcagc
    13081 catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta attcacgaca
    13141 gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca tgataaaagc
    13201 agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag
    13261 gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta aagcaaaaat
    13321 aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa attgtcccca
    13381 atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc taagtacagt
    13441 taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac gtgactatca
    13501 gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag gagtattttt
    13561 attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt gtcacgatcc
    13621 ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat cattctgcgt
    13681 attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga ttaactggta
    13741 cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg atggtcgcat
    13801 ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt gtcccttgat
    13861 aaacataggc attgtctact agcacaccta accatacacg attgacagca gtaaattcaa
    13921 gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca gaatcttctc
    13981 ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct actgtagaag
    14041 actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat ccttctactt
    14101 taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc caagtcatat
    14161 agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg
    14221 tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa gtacttactg
    14281 tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca tcaaaaacat
    14341 cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata aatgcccgca
    14401 cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc aaataacgtt
    14461 tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc aggcgtgctt
    14521 gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca gtctgtttga
    14581 ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt
    14641 catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc tcggtcacaa
    14701 gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg tgactcggag
    14761 ttaaacggca ggctcacgac ctctttctta aacggtgttc aatcagctgt tcctcggtat
    14821 tagctcaaaa accggcaaaa tataaggaga tatttttccg t
//
LOCUS       DACCSK010000069        14834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCSK010000069 DACCSK010000000
VERSION     DACCSK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.070"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(78..150)
                     /locus_tag="IVP39_001828"
     tRNA            complement(78..150)
                     /locus_tag="IVP39_001828"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IVP39_001829"
     CDS             531..1100
                     /locus_tag="IVP39_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7586899.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IVP39_001830"
     CDS             1087..1947
                     /locus_tag="IVP39_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7586900.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IVP39_001831"
     CDS             2137..2841
                     /locus_tag="IVP39_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7586901.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IVP39_001832"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IVP39_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7586902.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IVP39_001833"
     CDS             4678..5994
                     /locus_tag="IVP39_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586903.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IVP39_001834"
     CDS             5995..6864
                     /locus_tag="IVP39_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586904.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IVP39_001835"
     CDS             6919..7728
                     /locus_tag="IVP39_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7586905.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IVP39_001836"
     CDS             complement(7831..8460)
                     /locus_tag="IVP39_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP7586906.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IVP39_001837"
     CDS             8630..9922
                     /locus_tag="IVP39_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7586907.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IVP39_001838"
     CDS             complement(9998..10660)
                     /locus_tag="IVP39_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7586908.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IVP39_001839"
     CDS             10885..11169
                     /locus_tag="IVP39_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7586909.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IVP39_001840"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IVP39_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7586910.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(12934..14082)
                     /locus_tag="IVP39_001841"
     CDS             complement(12934..14082)
                     /locus_tag="IVP39_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7586911.1"
                     /translation="MLKVWVEQTKDGRFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFDELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYSSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRLTDPDQYIFISTSGTPLTLHSFNAVLHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14184..14768)
                     /locus_tag="IVP39_001842"
     CDS             complement(14184..14768)
                     /locus_tag="IVP39_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP7586912.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         4805 a         2566 c         3107 g         4356 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaagt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggcatga
    12841 tgtagtaatt aagaaagaac cctattttaa cagggttctt tcttcttttt ttgttttatt
    12901 ttaagtaaaa ggggcaaaga aggggcaaaa atatcaaagg ctatttagtt tatcaattac
    12961 ttgttgtttg gctcttttgg ttacatggtt atagatagac agagttgtat ttgcatcaga
    13021 atgtcctaca cgttccataa tggctttaag aggtacgccc aattcagata atagtgaaac
    13081 atggctatgt ctaaatatat gcgatgatag acttttttct aactccaatt cttcctctac
    13141 tttatggagt acagcgttaa atgagtgcag cgtaagcgga gtgccacttg tagatataaa
    13201 tatatattga tcgggatctg tgagtctacc cgcaagaatg ttgtcagcta tcacactttc
    13261 aatcaattct tttgcacgat tgggcaattg tacttcgcgt tgcgaataag tattttttgg
    13321 agttgttttt atagcattat ccattttcac agatgagtaa tctaaggtac cgttaatgga
    13381 aattttccca tcctcatagt ccttcatttg taaagctagc aattccccat atctcaaacc
    13441 agtcaaatat aaaaattcag ctattatgcc gtgtagtttt ctgcgaggat tggagtatag
    13501 ctgtttcagt atttgatcaa tttcttcttt ctccagatat tttttatcca tagaaagctt
    13561 tcttttttct tcttccactt ttttagggtg gattttaact gctagagcag ggtttctttg
    13621 gatgtatttt ctatcgattg catagtttag cataacagat agagttgttt ttgtttgttt
    13681 tgtgtagttc agtgagaggt caccaaacgt atacatatct tcaattatct tattaatgag
    13741 tgtctcatct atgtttctaa cgattgtatc atcgcttatg tgcttagaaa catgtttcat
    13801 catcattggc accttcagat agctagtacg tttaacatgc tgcttataat attcatacca
    13861 ttctttatac agctcatcaa aagtgatatc tgatttattg taattttcga gtgcttcttt
    13921 aattttttta tctagaattt tctgagcttt tttccacgct tgtggtgaat tacttgtaag
    13981 tgttgtagat ttttttcgtg ttttttctgt ataaggatct acatatcttt caataaactt
    14041 gaatcgccca tctttggttt gttcaaccca cacttttaac atctcctatc atttgctata
    14101 ataggcataa caaatagacc tatataggtt tgttttctaa aagcacgctc ttactctgga
    14161 cggtggggcg tgttttttat ttattataca gttgctttaa aaatagctgc tttttcaaat
    14221 gatactaaag gagagaagtg gatttctatt tctccctttt ggtttaacgt gaaatgagca
    14281 gttacatcca tttgttttcc tggagcaaca gatcccattg tattttcgtt ggcatatgtc
    14341 tctgattttt tgtcatctgg tccatatact tccacatctg tacctacagg gatatctgaa
    14401 tcgccatcat tttttacggt ataagtaatt tttactactt gtgcaggttg gttttcttca
    14461 aattgatttc tttcatcagt tagttctaca ctatttagtg tatattcagc atcaccaact
    14521 tttacagtgt caccaatctt atagaaggta tcgctttttt cttctttaga agatgaagca
    14581 gaggttgaag atttagttac tttttcgcca ccattatcac ttgctttatt actatctgat
    14641 ccaccattta aagcagaacc aataattata attaaaatta ctgctaatac ccaaaaccat
    14701 acttttttgt aaaaaggttt actaacttta tacatttttc cgtcttgacc cataactttt
    14761 tttgccattt aaatattcct cacttcttgt tataatatat ttgcgatctc agaaatgagg
    14821 tatgagtccg tgtg
//
LOCUS       DACCSK010000070        14567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCSK010000070 DACCSK010000000
VERSION     DACCSK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.071"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IVP39_001843"
     CDS             complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IVP39_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7586913.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1993..3216)
                     /locus_tag="IVP39_001844"
     CDS             complement(1993..3216)
                     /locus_tag="IVP39_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7586914.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3247..4086)
                     /locus_tag="IVP39_001845"
     CDS             complement(3247..4086)
                     /locus_tag="IVP39_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7586915.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4103..4783)
                     /locus_tag="IVP39_001846"
     CDS             complement(4103..4783)
                     /locus_tag="IVP39_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586916.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4785..5858)
                     /locus_tag="IVP39_001847"
     CDS             complement(4785..5858)
                     /locus_tag="IVP39_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7586917.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6505..7176)
                     /locus_tag="IVP39_001848"
     CDS             complement(6505..7176)
                     /locus_tag="IVP39_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7586918.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IVP39_001849"
     CDS             complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IVP39_001849"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7586919.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10600..11037
                     /gene="ndk"
                     /locus_tag="IVP39_001850"
     CDS             10600..11037
                     /gene="ndk"
                     /locus_tag="IVP39_001850"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7586920.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11059..11925)
                     /locus_tag="IVP39_001851"
     CDS             complement(11059..11925)
                     /locus_tag="IVP39_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7586921.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12085..14178)
                     /locus_tag="IVP39_001852"
     CDS             complement(12085..14178)
                     /locus_tag="IVP39_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7586922.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4229 a         3109 c         2480 g         4749 t
ORIGIN      
        1 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
       61 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
      121 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
      181 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
      241 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
      301 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
      361 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
      421 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
      481 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
      541 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
      601 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
      661 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
      721 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
      781 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
      841 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
      901 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
      961 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     1021 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     1081 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     1141 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     1201 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     1261 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     1321 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     1381 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     1441 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     1501 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     1561 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     1621 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     1681 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     1741 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     1801 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     1861 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     1921 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     1981 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     2041 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     2101 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     2161 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     2221 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     2281 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     2341 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     2401 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     2461 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     2521 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     2581 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     2641 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     2701 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     2761 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     2821 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     2881 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     2941 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     3001 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     3061 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     3121 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     3181 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     3241 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     3301 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     3361 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     3421 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     3481 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     3541 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     3601 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     3661 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     3721 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     3781 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
     3841 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
     3901 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
     3961 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
     4021 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
     4081 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
     4141 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
     4201 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
     4261 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
     4321 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
     4381 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
     4441 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
     4501 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
     4561 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
     4621 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
     4681 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
     4741 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
     4801 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
     4861 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
     4921 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
     4981 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
     5041 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
     5101 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
     5161 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
     5221 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
     5281 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
     5341 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
     5401 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
     5461 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
     5521 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
     5581 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
     5641 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
     5701 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
     5761 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
     5821 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
     5881 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
     5941 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
     6001 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
     6061 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
     6121 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
     6181 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
     6241 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
     6301 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
     6361 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
     6421 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
     6481 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
     6541 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
     6601 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
     6661 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
     6721 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
     6781 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
     6841 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
     6901 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
     6961 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
     7021 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
     7081 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
     7141 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
     7201 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
     7261 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
     7321 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
     7381 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
     7441 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
     7501 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
     7561 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
     7621 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
     7681 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
     7741 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
     7801 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
     7861 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
     7921 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
     7981 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
     8041 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
     8101 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
     8161 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
     8221 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
     8281 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
     8341 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
     8401 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
     8461 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
     8521 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
     8581 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
     8641 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
     8701 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
     8761 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
     8821 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
     8881 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
     8941 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
     9001 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
     9061 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
     9121 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
     9181 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
     9241 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
     9301 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
     9361 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
     9421 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
     9481 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
     9541 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
     9601 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
     9661 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
     9721 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
     9781 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
     9841 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
     9901 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
     9961 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    10021 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    10081 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    10141 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    10201 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    10261 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    10321 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    10381 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    10441 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    10501 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    10561 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    10621 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    10681 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    10741 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    10801 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    10861 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    10921 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    10981 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    11041 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    11101 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    11161 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    11221 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    11281 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    11341 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    11401 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    11461 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    11521 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    11581 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    11641 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    11701 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    11761 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    11821 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    11881 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    11941 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    12001 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    12061 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    12121 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    12181 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    12241 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    12301 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    12361 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    12421 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    12481 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    12541 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    12601 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    12661 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    12721 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    12781 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    12841 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    12901 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    12961 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    13021 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    13081 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    13141 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    13201 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    13261 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    13321 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    13381 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    13441 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    13501 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    13561 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    13621 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    13681 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    13741 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    13801 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    13861 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    13921 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    13981 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    14041 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    14101 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    14161 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    14221 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    14281 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    14341 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    14401 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    14461 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    14521 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattg
//
LOCUS       DACCSK010000071        14288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCSK010000071 DACCSK010000000
VERSION     DACCSK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.072"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..201)
                     /locus_tag="IVP39_001853"
     CDS             complement(<1..201)
                     /locus_tag="IVP39_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7586923.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFT"
     gene            complement(223..1197)
                     /locus_tag="IVP39_001854"
     CDS             complement(223..1197)
                     /locus_tag="IVP39_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7586924.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1497..2369
                     /locus_tag="IVP39_001855"
     CDS             1497..2369
                     /locus_tag="IVP39_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7586925.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIIIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(2951..5149)
                     /locus_tag="IVP39_001856"
     CDS             complement(2951..5149)
                     /locus_tag="IVP39_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7586926.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            5381..6373
                     /locus_tag="IVP39_001857"
     CDS             5381..6373
                     /locus_tag="IVP39_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7586927.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            6433..7854
                     /locus_tag="IVP39_001858"
     CDS             6433..7854
                     /locus_tag="IVP39_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7586928.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPDKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            7847..9346
                     /locus_tag="IVP39_001859"
     CDS             7847..9346
                     /locus_tag="IVP39_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7586929.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            9385..10779
                     /gene="uxaC"
                     /locus_tag="IVP39_001860"
     CDS             9385..10779
                     /gene="uxaC"
                     /locus_tag="IVP39_001860"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7586930.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            10938..11621
                     /locus_tag="IVP39_001861"
     CDS             10938..11621
                     /locus_tag="IVP39_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7586931.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            11623..12459
                     /locus_tag="IVP39_001862"
     CDS             11623..12459
                     /locus_tag="IVP39_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7586932.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            12470..14224
                     /locus_tag="IVP39_001863"
     CDS             12470..14224
                     /locus_tag="IVP39_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586933.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         4463 a         2769 c         2646 g         4410 t
ORIGIN      
        1 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
       61 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
      121 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
      181 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
      241 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
      301 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
      361 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
      421 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
      481 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
      541 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaagataga tgatcgtaga
      601 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
      661 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
      721 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
      781 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
      841 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaaaatcaa
      901 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
      961 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
     1021 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
     1081 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
     1141 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
     1201 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
     1261 ttacgtatat aatcattttt atcgtatgta aaaacgctta catagaaatg atttttgtgc
     1321 tatgcaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
     1381 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
     1441 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
     1501 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
     1561 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
     1621 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
     1681 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
     1741 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
     1801 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
     1861 gcggggggat gattccaacg ttcttagttg ttcgttctct aggactggtc aattcttact
     1921 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
     1981 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
     2041 gtgtattttt caaaattatg ctgccattag cgttaccgtc tattgctact atctcgcttt
     2101 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
     2161 ctaaatggcc gatacagatt cttttacgcc aaatcatcat tgtatcaagt gggatgcaag
     2221 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
     2281 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
     2341 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
     2401 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
     2461 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
     2521 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
     2581 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
     2641 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
     2701 gacttttgtc acagctcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
     2761 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
     2821 cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc agcagcaaaa
     2881 catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac aagaaaaaca
     2941 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
     3001 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
     3061 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
     3121 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
     3181 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
     3241 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
     3301 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
     3361 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
     3421 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
     3481 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
     3541 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
     3601 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
     3661 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
     3721 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
     3781 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
     3841 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
     3901 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
     3961 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
     4021 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
     4081 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
     4141 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
     4201 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
     4261 gggcaaatct gtccgaaaat aagtaggaaa gaagaccaat gagtcctacc agtgttaaag
     4321 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
     4381 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
     4441 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
     4501 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
     4561 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
     4621 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
     4681 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
     4741 caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
     4801 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
     4861 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
     4921 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
     4981 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
     5041 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
     5101 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
     5161 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
     5221 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
     5281 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
     5341 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatat
     5401 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
     5461 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
     5521 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
     5581 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
     5641 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
     5701 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
     5761 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
     5821 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
     5881 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
     5941 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
     6001 caatccagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
     6061 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
     6121 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
     6181 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
     6241 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
     6301 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
     6361 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
     6421 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
     6481 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
     6541 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
     6601 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
     6661 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
     6721 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
     6781 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
     6841 cccccacaga gcttcccgga caaactgaca gcctttttat accgacgttt caaattaaac
     6901 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
     6961 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
     7021 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
     7081 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
     7141 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
     7201 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
     7261 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
     7321 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
     7381 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
     7441 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
     7501 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
     7561 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
     7621 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
     7681 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
     7741 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
     7801 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
     7861 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
     7921 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
     7981 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
     8041 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
     8101 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
     8161 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
     8221 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
     8281 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
     8341 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
     8401 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
     8461 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
     8521 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
     8581 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
     8641 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
     8701 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
     8761 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
     8821 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
     8881 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
     8941 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
     9001 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
     9061 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
     9121 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
     9181 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
     9241 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
     9301 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
     9361 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
     9421 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
     9481 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
     9541 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
     9601 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
     9661 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
     9721 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
     9781 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
     9841 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
     9901 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
     9961 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    10021 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    10081 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    10141 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatcgt
    10201 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    10261 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    10321 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaatcgt
    10381 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    10441 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    10501 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    10561 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    10621 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    10681 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    10741 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    10801 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    10861 cggaccaaat ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt
    10921 atgaaagaag agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa
    10981 aagatgaaaa caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac
    11041 ccgagatcca gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg
    11101 aaggaaggtt agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat
    11161 ttggacataa cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt
    11221 taaaaaatct gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac
    11281 tgacatctgt gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg
    11341 gagactatcc aaaagcaatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg
    11401 tgttctcaag atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct
    11461 acctgcatct tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc
    11521 actttagtcc agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat
    11581 cccgtctgcc tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta
    11641 ggcatccgtg cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca
    11701 cagcttcact tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa
    11761 ttaactgaca tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat
    11821 atcgaacttt ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt
    11881 gcacaagcat tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag
    11941 atgattgcta cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa
    12001 gaagcttatc agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt
    12061 ggcgtaactg ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct
    12121 aaacgtttaa tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat
    12181 ttgatgcata aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat
    12241 ttacatggtt atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc
    12301 cgcatggtac ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa
    12361 tatgagcgtc ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa
    12421 gcaatagaac acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg
    12481 aatacagaaa agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta
    12541 tcagggaaat tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc
    12601 atcttggtta atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc
    12661 ttctcagttg caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa
    12721 tatattgacg aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc
    12781 tggactaaga gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga
    12841 attgcaatct ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt
    12901 gtcctctcaa gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg
    12961 tatcacgctt tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc
    13021 cgcattcgtt actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca
    13081 agaaatggaa gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg
    13141 agctcatggg ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta
    13201 tgaaaaagga ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag
    13261 ttttcagata tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg
    13321 catccaaccc ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt
    13381 ttctggaatg atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat
    13441 tggcgaactg ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca
    13501 tttggatgga aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc
    13561 gattatcgat catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc
    13621 atctggctct ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc
    13681 aggaaaaatt tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa
    13741 ttatttagcc gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat
    13801 cacttatggc ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca
    13861 actttcttca ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg
    13921 caacaagctg tctggtggcc aaaagcaacg tatctccatt gcacgagcgt tgatacgtca
    13981 gccaaaaatc attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa
    14041 aatccagcaa gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg
    14101 gctttcaacg atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaattgttga
    14161 aataggtact tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga
    14221 atgacaaaaa ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg
    14281 gtgttctc
//
LOCUS       DACCSK010000072        14030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCSK010000072 DACCSK010000000
VERSION     DACCSK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.073"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(74..1030)
                     /locus_tag="IVP39_001864"
     CDS             complement(74..1030)
                     /locus_tag="IVP39_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7586934.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="IVP39_001865"
     CDS             1134..1736
                     /locus_tag="IVP39_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7586935.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="IVP39_001866"
     CDS             complement(1824..3323)
                     /locus_tag="IVP39_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7586936.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="IVP39_001867"
     CDS             complement(3339..3863)
                     /locus_tag="IVP39_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586937.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="IVP39_001868"
     CDS             4161..4910
                     /locus_tag="IVP39_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7586938.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="IVP39_001869"
     CDS             4901..5686
                     /locus_tag="IVP39_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7586939.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="IVP39_001870"
     CDS             5655..6371
                     /locus_tag="IVP39_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7586940.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="IVP39_001871"
     CDS             complement(6473..7492)
                     /locus_tag="IVP39_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7586941.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8569)
                     /locus_tag="IVP39_001872"
     CDS             complement(7511..8569)
                     /locus_tag="IVP39_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7586942.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8790..9938)
                     /gene="nagA"
                     /locus_tag="IVP39_001873"
     CDS             complement(8790..9938)
                     /gene="nagA"
                     /locus_tag="IVP39_001873"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7586943.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10014..10850)
                     /locus_tag="IVP39_001874"
     CDS             complement(10014..10850)
                     /locus_tag="IVP39_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7586944.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11034..12251)
                     /locus_tag="IVP39_001875"
     CDS             complement(11034..12251)
                     /locus_tag="IVP39_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7586945.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12634..12717
                     /locus_tag="IVP39_001876"
     tRNA            12634..12717
                     /locus_tag="IVP39_001876"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12668..12670,aa:Leu,seq:gag)
     gene            complement(12957..13955)
                     /locus_tag="IVP39_001877"
     CDS             complement(12957..13955)
                     /locus_tag="IVP39_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586946.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4278 a         2845 c         2391 g         4516 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
     8641 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
     8701 aacttatgta aagtgatttt tactagaaca caaaaaaaac cttaaaacca aacttaagaa
     8761 gtttagtttt aaggttaact taaattatat tatgcgtgga aacgttccac gccatctaga
     8821 taagttgctt ccaatgtcat atcagggttc aatacgataa agtctgcatc gcgtccttga
     8881 gcgatttttc cacatgtatc atcgatttta cagctgattg ctggaatcag actagccatc
     8941 atgactgctt gttctggtgt tgctaatccc cagtctacaa catttttaat tgcttctttc
     9001 aatttcaata tacttccagc taagttgcca gattctagac gtgctgttcc ttcttttact
     9061 actactggaa attcgcctaa attgtaattt ccatcaggca tcccaccggc catcatacag
     9121 tctgtaatta atgcgacatg atcatgtcct gctttttcca ttaggattcc cgcagcattt
     9181 ggatgtacat gatggccatc acaaatcaat tcagaaaaga catgttctaa agataataac
     9241 gcacccacca tacctggttc gcgatgattc aatccacgca taccattgaa agcatggaca
     9301 aatacacttg cgcctgcttc aacagctttt tgcgcttctt ctagtgtagc attactgtgt
     9361 cctagagaaa caactactcc ttcttcagtc acttgtttga caaactcttc tacaccttga
     9421 cgttccggtg ctaaagcaat ttttttgatt aaaccacctg aagcttcttg ccattcatgg
     9481 aaagtatcca aatcaggatc cccgaaataa cttgggtttt gtgccccttt gtgttcttct
     9541 gtgaagaaag gaccttcaaa atagattcct ttgattttag ctcctggtgc ttcctgatac
     9601 attttgccga ttgtctcagc tacatctttt aaacgttctt tgcttgacgt caatgttgtt
     9661 ggtaaaaatg aagtcactcc acaagataac agaccttcag acatcacttt gatgccttct
     9721 gcatcattat ccattacatc atgattcata tagccgtgaa tatgtgtatc taccaaacca
     9781 ggagcaatcc atttgccgct ttggtcaatg atgttgactt ctccctcagg tagttctttg
     9841 gtgtattcac caaaaagtcc atcggtaatt tcaagatagc ctgcatgttt gacacctgac
     9901 gctaaaaaga atttgtcagc atagataaaa gttctcattc tattctctcc ctttgttttt
     9961 ctacctctaa agtactctta tttagagaag aagtcaagaa tggtctacac catttacaaa
    10021 ttttttgaaa tttgtttcac agcttgtgct aagccttcat agaatgctgc tctttctggt
    10081 tcttctttac gcttgtatat taagctttta agcacaaata aatcattgag gtaatagtag
    10141 ctattcttct cttgagccca atagattcct tgattcacct gttctaacgc ttcgtcgatt
    10201 tcattttgat ggaataagaa atttccataa ttataaaaaa tttgcgaaaa ttcacttttt
    10261 gatcgttcat cagctgattg atgcagcagt tggatgcttc gactcaaata gtagcgtgct
    10321 tcagccgttt ttcctgccac accatatatt tttccgatac aactaatcag ttgtatttct
    10381 gtgtcagaaa catggattct ttctttttgg ctagagtagc ttaatgcttc tttcaaataa
    10441 taaagggact gagagatatt ttgacgcaac gtgaattcgc aaacaccgag ataataataa
    10501 tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa tatcagaaga ttttagtact
    10561 tgttctaact tccgataatc acgtttttgg aaacaactat ccaacagttc aaataattta
    10621 tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat gatcaatcgt tacccctagc
    10681 cgttgacaaa tctgcatcat caccaataca ttcggcagtt cctcattatt ttcgatccga
    10741 cttaatacac tttgtgaaca aatatcatgc gaaagcattt tttgcgtcat tttttgttct
    10801 tttcgaatct ctttaatgat cgttccaaag gactcgatta acgtttccat aagtatcctc
    10861 cccaaaatat gcgtattcgc attctattcg tattttactt taggaagtcg caaacttcaa
    10921 gaaaagactt gcaactaaat aatatacttt agttatttaa gaaaactcca ctagaaagag
    10981 agcctcagtg tgtttgtctg tggccctcct actgaaaaat ttggttttta aaatcatttt
    11041 tggcgatagg ttgctaggaa ttctctcatt ttcttagctg tttgtttcaa atcatccatt
    11101 ttaggaagat aaacgattcg aaaatgatct ggctggttcc aattaaatcc accaccatgt
    11161 acaagtagaa tatgatgttc atgtaaaaag tccaaaacga atttttcatc atccaaaata
    11221 ttgaatcgct ttgtatcaat ttttgggaat atataaaaag cagcttttgg ttttacggct
    11281 gaaagaccag gaatatcatt gattgcatta tagatatatt ctctttgttc atagatcctt
    11341 ccgcctggca ggagcagctc atcgacactt tgataaccgc ctagcgcggt ttggataatt
    11401 tgctgagaca atacattgga acacaaacgc atggaggaca acatattcag cccttctata
    11461 tagcctttga cgtgtgactt atctccactt aggaccatcc agcctactcg aaatcctgct
    11521 acacgatgag atttggatag accgttcagc gtaacaacga acaaatcagg cgctaatgta
    11581 gcaatcggaa tatgttctag accatccatc actaagcgat catagatttc gtcggaataa
    11641 ataatcaggt tattttgtcg agctacttca acgatttctt ctaataattc ttttggataa
    11701 agtgccccag taggattatt aggattaatg ataacaatag cttttgtccg actcgttatt
    11761 tttgatttga tatcatcaat atcaggatac cattctgcct cttcatcgca aatataatga
    11821 acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca acggatagtc cggcattgga
    11881 accagtactt catctccatt gtccagcaac ccctgcatac acatcgtgat caattcgctc
    11941 actccgttgc cggtgtagat atcattgatc gtcacattcg gaaaatgttt cagttggcaa
    12001 tattgctcaa ttgcttttct agcagaaaag atccccttgg aatctgagta gccttctgag
    12061 tcacgggcat tcatgatcaa atcacgaatg acttcgtttg gtgcttcaaa tccaaaaggt
    12121 gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct cttcttgcat ccgctccgct
    12181 tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt ctaatttgtt ggatttttca
    12241 aattctctca tcacttagcc aatccctcat ttcaaaaagt tagaactttc caaatttttt
    12301 gttatttact atttcaatat catactccat cctagaaaat tttgccactt atttaggaaa
    12361 aacgtaagta ttaaaaaaac acaaacaagc gttgagaact taattgtcat gatagtcatt
    12421 ccatagattc gctgcccaat ccattgcaat tccacaaatg attcctagca taaaaccaag
    12481 caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag atcattccca gtcctgaaaa
    12541 ttctccgtca tatttctcca tcttaataag tcctcccatt tttgagcttg acaactatgt
    12601 aaaacctcgc tatcattagt gtgttattat tacgcagatg tgttggaatt ggcagacaag
    12661 catgattgag ggtcatgtga gcgttagctc gtgtgggttc gagtcccatc atctgcacta
    12721 caactcgtga agttaatttt gcttcacgag tttttttgtt tgaacgtttc tttatgccat
    12781 tatatacata tcactgtatc ctttacaaaa agaacgtaca atcatgtaaa cagcctgagg
    12841 tgagtatatg aaggtcggaa acaagctact cgtagaaact tgcaagtgtt aaaacaatta
    12901 atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg agggattttt tgtatattat
    12961 ctgtttatca ccgttattat aaaggtacaa ataccaagca cggttaatcc aaattgcata
    13021 aattcatttt tttgtacact catgcttttc attagctctt taactgccat tagtactaag
    13081 atggcgataa caacataaag tatatagtac gtccgcattc cggaagccca tactgtggga
    13141 gaaaatccca taattaacct tgaacaaaat cccattatca tcaaaataac taaaaaaata
    13201 gaagttttct tattgtcaaa acttaaataa attccaacaa ttaaacatag taataatgcc
    13261 aagactacta aagtagctat ccaagtacct ggataaaaaa gtgacagttt cgttcctaac
    13321 tctgtaaaga gattattcag gttactacta ctccaaatca ttgccctttt atttccattg
    13381 acgtaagtaa agctttgacc cattgtatta ccaaaataaa taatcaggtt taagaaaaac
    13441 ggtaatgcag tcaatatacg cacataatag ttttgacatt tcctatatgt tagaaggaaa
    13501 attaagaaaa atagcaataa aaataagata ttcatgtcta aaaaaaacgg ctttccaaat
    13561 gatgaaatac ctaaatctaa cttattaaga aagttgaaat ttttatattc aggaaaccat
    13621 ttattaattt catgtgcagc acgtaatgca ttccctggag ttgtcaaaga gaaaattaat
    13681 tctgctaagc ttattattga aaatactgat aacttataat tatatcgtcc attgacaatc
    13741 aaatataaac taactatact tgttagaaca aaaaaacagg cgttgacttg ttcttgatta
    13801 gttaaaaata tcaataaagg aatacttacc caatatattt ttctatttat ttcttctcct
    13861 cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa atgctactgg ccactgatag
    13921 ttcaaggtgg ttgctctcca accggtttcc cccattactg tcaatggtat taaacagtat
    13981 atagagcaaa ccaataataa atttttaaca tttattttct caaaaacata
//
LOCUS       DACCSK010000073        13992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCSK010000073 DACCSK010000000
VERSION     DACCSK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.074"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1469
                     /locus_tag="IVP39_001878"
     CDS             <1..1469
                     /locus_tag="IVP39_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7586947.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
     gene            complement(1599..4295)
                     /locus_tag="IVP39_001879"
     CDS             complement(1599..4295)
                     /locus_tag="IVP39_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7586948.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(4723..5796)
                     /locus_tag="IVP39_001880"
     CDS             complement(4723..5796)
                     /locus_tag="IVP39_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7586949.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(5793..6620)
                     /locus_tag="IVP39_001881"
     CDS             complement(5793..6620)
                     /locus_tag="IVP39_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586950.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(6621..7427)
                     /locus_tag="IVP39_001882"
     CDS             complement(6621..7427)
                     /locus_tag="IVP39_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7586951.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(7439..8524)
                     /locus_tag="IVP39_001883"
     CDS             complement(7439..8524)
                     /locus_tag="IVP39_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7586952.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(8541..9083)
                     /locus_tag="IVP39_001884"
     CDS             complement(8541..9083)
                     /locus_tag="IVP39_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7586953.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            9341..10330
                     /locus_tag="IVP39_001885"
     CDS             9341..10330
                     /locus_tag="IVP39_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7586954.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10327..11064
                     /locus_tag="IVP39_001886"
     CDS             10327..11064
                     /locus_tag="IVP39_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7586955.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            11061..11612
                     /gene="coaC"
                     /locus_tag="IVP39_001887"
     CDS             11061..11612
                     /gene="coaC"
                     /locus_tag="IVP39_001887"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7586956.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            11628..12260
                     /locus_tag="IVP39_001888"
     CDS             11628..12260
                     /locus_tag="IVP39_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7586957.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            12345..12992
                     /locus_tag="IVP39_001889"
     CDS             12345..12992
                     /locus_tag="IVP39_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7586958.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            12997..13980
                     /locus_tag="IVP39_001890"
     CDS             12997..13980
                     /locus_tag="IVP39_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7586959.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         4354 a         2833 c         2542 g         4263 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgtac aggtactgtt ctatggggcg cttacttaaa aaaagtgtct
     1561 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
     1621 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
     1681 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
     1741 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
     1801 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
     1861 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
     1921 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
     1981 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
     2041 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
     2101 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
     2161 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
     2221 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
     2281 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
     2341 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
     2401 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
     2461 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
     2521 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
     2581 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
     2641 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
     2701 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
     2761 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
     2821 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
     2881 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
     2941 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
     3001 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
     3061 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
     3121 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
     3181 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
     3241 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
     3301 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
     3361 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
     3421 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
     3481 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
     3541 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
     3601 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
     3661 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
     3721 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
     3781 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
     3841 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
     3901 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
     3961 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
     4021 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
     4081 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
     4141 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
     4201 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
     4261 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
     4321 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
     4381 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
     4441 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
     4501 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
     4561 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
     4621 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
     4681 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
     4741 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
     4801 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
     4861 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
     4921 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
     4981 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
     5041 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
     5101 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
     5161 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
     5221 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
     5281 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
     5341 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
     5401 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
     5461 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
     5521 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
     5581 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
     5641 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
     5701 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
     5761 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
     5821 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
     5881 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
     5941 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
     6001 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
     6061 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
     6121 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
     6181 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
     6241 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
     6301 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
     6361 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
     6421 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
     6481 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
     6541 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
     6601 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
     6661 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
     6721 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
     6781 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
     6841 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
     6901 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
     6961 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
     7021 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
     7081 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
     7141 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
     7201 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
     7261 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
     7321 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
     7381 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
     7441 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
     7501 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
     7561 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
     7621 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
     7681 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
     7741 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
     7801 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
     7861 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
     7921 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
     7981 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
     8041 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
     8101 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
     8161 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
     8221 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
     8281 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
     8341 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
     8401 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
     8461 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
     8521 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
     8581 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
     8641 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
     8701 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
     8761 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
     8821 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
     8881 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
     8941 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
     9001 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
     9061 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
     9121 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
     9181 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
     9241 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
     9301 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
     9361 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
     9421 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
     9481 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
     9541 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
     9601 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
     9661 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
     9721 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
     9781 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
     9841 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
     9901 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
     9961 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    10021 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    10081 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    10141 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    10201 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    10261 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    10321 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    10381 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    10441 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    10501 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    10561 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    10621 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    10681 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    10741 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    10801 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    10861 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    10921 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    10981 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    11041 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    11101 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    11161 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    11221 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    11281 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    11341 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    11401 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    11461 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    11521 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    11581 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    11641 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    11701 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    11761 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    11821 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    11881 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    11941 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    12001 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    12061 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    12121 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    12181 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    12241 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    12301 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    12361 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    12421 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    12481 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    12541 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    12601 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    12661 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    12721 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    12781 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    12841 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    12901 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    12961 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    13021 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    13081 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    13141 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    13201 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    13261 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    13321 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    13381 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    13441 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    13501 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    13561 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    13621 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    13681 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    13741 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    13801 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    13861 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    13921 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    13981 aatcatgctg at
//
LOCUS       DACCSK010000074        13910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCSK010000074 DACCSK010000000
VERSION     DACCSK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.075"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IVP39_001891"
     CDS             complement(99..1229)
                     /locus_tag="IVP39_001891"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7586960.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVP39_001892"
     CDS             complement(1878..3080)
                     /locus_tag="IVP39_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7586961.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVP39_001893"
     CDS             complement(3140..3343)
                     /locus_tag="IVP39_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7586962.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVP39_001894"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVP39_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVP39_001895"
     CDS             4880..5692
                     /locus_tag="IVP39_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7586963.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="IVP39_001896"
     CDS             complement(6716..6913)
                     /locus_tag="IVP39_001896"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586964.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVP39_001897"
     CDS             complement(7059..7937)
                     /locus_tag="IVP39_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7586965.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVP39_001898"
     CDS             complement(7976..9094)
                     /locus_tag="IVP39_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7586966.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVP39_001899"
     CDS             complement(9091..11190)
                     /locus_tag="IVP39_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7586967.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVP39_001900"
     CDS             complement(11241..12062)
                     /locus_tag="IVP39_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7586968.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVP39_001901"
     CDS             complement(12059..12868)
                     /locus_tag="IVP39_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7586969.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVP39_001902"
     CDS             complement(12889..13383)
                     /locus_tag="IVP39_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7586970.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVP39_001903"
     CDS             complement(13395..13883)
                     /locus_tag="IVP39_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7586971.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         4222 a         3016 c         2248 g         4424 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa
//
LOCUS       DACCSK010000075        13896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCSK010000075 DACCSK010000000
VERSION     DACCSK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.076"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            133..507
                     /locus_tag="IVP39_001904"
     CDS             133..507
                     /locus_tag="IVP39_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7586972.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IVP39_001905"
     CDS             complement(621..1250)
                     /locus_tag="IVP39_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7586973.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IVP39_001906"
     tRNA            complement(1384..1456)
                     /locus_tag="IVP39_001906"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IVP39_001907"
     tRNA            1670..1743
                     /locus_tag="IVP39_001907"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            1698..1847
                     /locus_tag="IVP39_001908"
     CDS             1698..1847
                     /locus_tag="IVP39_001908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586974.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2340..3668
                     /locus_tag="IVP39_001909"
     CDS             2340..3668
                     /locus_tag="IVP39_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7586975.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="IVP39_001910"
     CDS             3870..4703
                     /locus_tag="IVP39_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7586976.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="IVP39_001911"
     CDS             4719..5456
                     /locus_tag="IVP39_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7586977.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="IVP39_001912"
     CDS             complement(5611..6579)
                     /locus_tag="IVP39_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7586978.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="IVP39_001913"
     CDS             6811..7644
                     /locus_tag="IVP39_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7586979.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="IVP39_001914"
     CDS             7678..8517
                     /locus_tag="IVP39_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7586980.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="IVP39_001915"
     CDS             8543..9103
                     /locus_tag="IVP39_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7586981.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="IVP39_001916"
     CDS             9302..11023
                     /locus_tag="IVP39_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7586982.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11188..12330
                     /locus_tag="IVP39_001917"
     CDS             11188..12330
                     /locus_tag="IVP39_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7586983.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12346..13557
                     /gene="thiI"
                     /locus_tag="IVP39_001918"
     CDS             12346..13557
                     /gene="thiI"
                     /locus_tag="IVP39_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7586984.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4745 a         2294 c         2826 g         4031 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat
    11101 tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa
    11161 aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca
    11221 atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca
    11281 tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag
    11341 atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa
    11401 ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg
    11461 atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta
    11521 ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg
    11581 aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta
    11641 ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat
    11701 ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa
    11761 agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc
    11821 atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga
    11881 aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt
    11941 ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta
    12001 atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca
    12061 ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta
    12121 gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca
    12181 agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc
    12241 agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag
    12301 ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa
    12361 attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg
    12421 caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct
    12481 gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa
    12541 ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc
    12601 gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca
    12661 tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat
    12721 caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa
    12781 ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg
    12841 atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct
    12901 ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa
    12961 gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat
    13021 ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc
    13081 gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt
    13141 cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg
    13201 aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat
    13261 gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt
    13321 gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt
    13381 acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat
    13441 gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa
    13501 gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag
    13561 gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat
    13621 gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga
    13681 agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca
    13741 agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac
    13801 agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg
    13861 tgaaatttcg gcttattttt cgaagaagtt acttct
//
LOCUS       DACCSK010000076        13865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCSK010000076 DACCSK010000000
VERSION     DACCSK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.077"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(6..662)
                     /locus_tag="IVP39_001919"
     CDS             complement(6..662)
                     /locus_tag="IVP39_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7586985.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            855..1133
                     /locus_tag="IVP39_001920"
     CDS             855..1133
                     /locus_tag="IVP39_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586986.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1152..2360
                     /locus_tag="IVP39_001921"
     CDS             1152..2360
                     /locus_tag="IVP39_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7586987.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2508..2873)
                     /locus_tag="IVP39_001922"
     CDS             complement(2508..2873)
                     /locus_tag="IVP39_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7586988.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3020..3793)
                     /locus_tag="IVP39_001923"
     CDS             complement(3020..3793)
                     /locus_tag="IVP39_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7586989.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3967..4827)
                     /locus_tag="IVP39_001924"
     CDS             complement(3967..4827)
                     /locus_tag="IVP39_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7586990.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4827..6245)
                     /locus_tag="IVP39_001925"
     CDS             complement(4827..6245)
                     /locus_tag="IVP39_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7586991.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6302..6751)
                     /locus_tag="IVP39_001926"
     CDS             complement(6302..6751)
                     /locus_tag="IVP39_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7586992.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7196..7459)
                     /locus_tag="IVP39_001927"
     CDS             complement(7196..7459)
                     /locus_tag="IVP39_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7586993.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7558..8853)
                     /locus_tag="IVP39_001928"
     CDS             complement(7558..8853)
                     /locus_tag="IVP39_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7586994.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8850..10121)
                     /locus_tag="IVP39_001929"
     CDS             complement(8850..10121)
                     /locus_tag="IVP39_001929"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7586995.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10286..11155)
                     /locus_tag="IVP39_001930"
     CDS             complement(10286..11155)
                     /locus_tag="IVP39_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7586996.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11447..13057)
                     /locus_tag="IVP39_001931"
     CDS             complement(11447..13057)
                     /locus_tag="IVP39_001931"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7586997.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3944 a         2920 c         2367 g         4634 t
ORIGIN      
        1 gttcatcaaa agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg
       61 tctttaacgg tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta
      121 gcagttgaga tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc
      181 atcccttctt ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca
      241 gctcccagct ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga
      301 tagcggtaat actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa
      361 aagcgcacac gattgattcg ataaaactct gcatattctg attcaactcg aatattatac
      421 ggcgaatcga tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg
      481 atatagcgaa ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc
      541 tctttcaaga tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat
      601 cctttgcgta taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc
      661 aatgatgctc ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt
      721 taaagtccaa aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac
      781 ttatgcgatt tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa
      841 tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat
      901 atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact
      961 cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa
     1021 atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca
     1081 attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat
     1141 gtataacata catggaaaag aaattattta ataaatattt tattggaatc acattgatca
     1201 attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag
     1261 acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa
     1321 cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct
     1381 taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta
     1441 tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag
     1501 ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt
     1561 attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc
     1621 tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta
     1681 cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat
     1741 ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa
     1801 ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa
     1861 aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca
     1921 catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt
     1981 atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg
     2041 tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg
     2101 gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct
     2161 actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat
     2221 ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag
     2281 cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat
     2341 cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa
     2401 taatctgagc ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt
     2461 tatttttaaa gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc
     2521 cgttcatctg gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca
     2581 ccgatcaata gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa
     2641 tatagataga gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg
     2701 ttccctactt tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt
     2761 accccaacaa tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac
     2821 agaatcgccc agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc
     2881 actcctttct cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat
     2941 accatcggct gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt
     3001 agaacattca cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg
     3061 catactgttc ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa
     3121 tggcttgatg catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt
     3181 ctgattgttt tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc
     3241 cttggctgaa atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg
     3301 catcgttata ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca
     3361 atacagatac ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg
     3421 taatgatctg caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac
     3481 tggaatccaa gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct
     3541 tacgtttttc tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt
     3601 ttcgattgtc aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca
     3661 gaaaaacaag gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg
     3721 tgactcttgc aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca
     3781 cttcattcaa cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt
     3841 gacagaagtg attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac
     3901 atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt
     3961 ttatttttag tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac
     4021 cacttcttgt acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg
     4081 attttcactc aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa
     4141 attcactttg gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga
     4201 accgccatgt aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat
     4261 atttaatttt ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc
     4321 aagtgtatcg attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat
     4381 gattgtttca gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc
     4441 cactgagaca tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc
     4501 gtcgaatgat ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg
     4561 taagacatct tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt
     4621 ataagcatag gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg
     4681 atgaatttct aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat
     4741 catatcatag ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt
     4801 caataactct ttcattgtat ataacattac gcttccacct catttgttag attttcattt
     4861 ttcgtccact tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag
     4921 gctgtagtga ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca
     4981 tttggaatta gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc
     5041 atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg
     5101 tcagataccg caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat
     5161 gattgacctg aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa
     5221 gcaataccta ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact
     5281 ccagctgaca tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata
     5341 tcagctgcca tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag
     5401 ctattcagcc aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat
     5461 accatcaaaa gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata
     5521 gcagaaaatt ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct
     5581 agcacgccac caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg
     5641 gcaacaattc ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct
     5701 aacgcaggaa ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct
     5761 aacacattat agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt
     5821 gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa
     5881 tgattataga ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg
     5941 aatacgccaa tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg
     6001 cttgtagaga cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct
     6061 actgcaagaa tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt
     6121 tttacacctt gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat
     6181 ttttctgccg acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt
     6241 ttcatgatta ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt
     6301 ttcattcttg actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct
     6361 ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat
     6421 ctgctgattt ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc
     6481 cgctatagtc cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt
     6541 tgctttttgc atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc
     6601 gtttgaatag tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca
     6661 catcccctat tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca
     6721 gttcttctgc gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta
     6781 acgttttctt ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa
     6841 tgaacagagg aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc
     6901 tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc
     6961 ttctaatttt aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat
     7021 aaggtgtttt tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca
     7081 ttatgatcct acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa
     7141 aaacagacta tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt
     7201 ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg
     7261 cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac
     7321 ggatgactgg gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg
     7381 aacctgacaa gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg
     7441 gatggatgtt ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag
     7501 agttgatttg tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta
     7561 tcttttcaga tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa
     7621 aaattgctga ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa
     7681 tgaatctcca gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat
     7741 caataattct tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg
     7801 ttcagataat tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata
     7861 gatcacgtca tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact
     7921 gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc
     7981 tgggtcaatc ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg
     8041 agcaagtcta gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg
     8101 cattgcgcgt tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa
     8161 tgtagaagat accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg
     8221 ttcatcaatc agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc
     8281 gatttctttt aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg
     8341 accaaaaccg attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt
     8401 ttccagttga agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc
     8461 ttttgcttct tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg
     8521 ttccgattct ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa
     8581 tcgtctgatt tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg
     8641 ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa
     8701 tccttgtttc tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt
     8761 actgtaataa ggaattttaa attctttggc ataactataa acttctttta acgttttgtt
     8821 ctctagttct gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca
     8881 attgatgccc ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa
     8941 tctacattgt aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca
     9001 cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg
     9061 atgatcatat ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg
     9121 ttcacacgtt tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt
     9181 aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg
     9241 tttgcgggtt ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata
     9301 tggacaccaa tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt
     9361 ttcactttga taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg
     9421 cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc
     9481 acatctgtac ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct
     9541 atgatttccg gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca
     9601 gctaacatca cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca
     9661 cgcaatcctt tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca
     9721 cctagagctt caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca
     9781 cctggcaggc ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa
     9841 gaagcacgca aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga
     9901 tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca
     9961 attaatgaat gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct
    10021 aagattgcag ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca
    10081 ccagctaaag gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat
    10141 taatcttttt tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg
    10201 gtctgccgtt ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta
    10261 tatatcgtat ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat
    10321 gagatttagc atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga
    10381 atcctttacc ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg
    10441 ataattggaa ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga
    10501 tttgatcttg aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag
    10561 catcagcaat tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac
    10621 catggatgtt accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc
    10681 attcttcaat atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg
    10741 aaccaacttc acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag
    10801 ctaatttcaa gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc
    10861 ctaattcgat acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg
    10921 gaacagtgat gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac
    10981 cacccatgta tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt
    11041 cagccgcttc aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag
    11101 cgtaaccgcc tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa
    11161 aattcctcct aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta
    11221 ttttaccaaa catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa
    11281 gttttttgca aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc
    11341 tatctttttt cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc
    11401 ttatcccctt ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt
    11461 gatctgctaa tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag
    11521 aaatcaattc tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga
    11581 tttctactaa acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca
    11641 attgacgata tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg
    11701 ctccatcata tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa
    11761 ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat
    11821 tgtttgctgt atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac
    11881 gtgcaaattc gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat
    11941 tttcgcgagc ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac
    12001 caggaaccaa gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt
    12061 cttgtgcttt gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca
    12121 atgcttcaac tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa
    12181 tccgtacttt tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt
    12241 ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt
    12301 gtaaaactaa tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg
    12361 gttcgacatc acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg
    12421 tacgtaccac caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct
    12481 gcgttggttt agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga
    12541 tatacataac attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg
    12601 gcaaagattc tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag
    12661 tcattttagc tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt
    12721 gaacagttgc tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt
    12781 ttcctgtcgt cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc
    12841 ccagatccaa atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg
    12901 ggctcatcgt ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta
    12961 agccgcggtt tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg
    13021 aaacgacacc gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat
    13081 ttatgtgcag gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg
    13141 gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa
    13201 gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt
    13261 tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc
    13321 acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt
    13381 actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa
    13441 tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc
    13501 gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca
    13561 cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc
    13621 actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa
    13681 ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt
    13741 ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca
    13801 tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc
    13861 tctgg
//
LOCUS       DACCSK010000077        13076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCSK010000077 DACCSK010000000
VERSION     DACCSK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.078"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(92..715)
                     /locus_tag="IVP39_001932"
     CDS             complement(92..715)
                     /locus_tag="IVP39_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7586998.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(874..2271)
                     /locus_tag="IVP39_001933"
     CDS             complement(874..2271)
                     /locus_tag="IVP39_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7586999.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2303..3274)
                     /locus_tag="IVP39_001934"
     CDS             complement(2303..3274)
                     /locus_tag="IVP39_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7587000.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3648..4073
                     /locus_tag="IVP39_001935"
     CDS             3648..4073
                     /locus_tag="IVP39_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587001.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4561..5262)
                     /locus_tag="IVP39_001936"
     CDS             complement(4561..5262)
                     /locus_tag="IVP39_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7587002.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5278..5718)
                     /locus_tag="IVP39_001937"
     CDS             complement(5278..5718)
                     /locus_tag="IVP39_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7587003.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5718..6293)
                     /locus_tag="IVP39_001938"
     CDS             complement(5718..6293)
                     /locus_tag="IVP39_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7587004.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7141..7620)
                     /locus_tag="IVP39_001939"
                     /pseudo
     CDS             complement(7141..7620)
                     /locus_tag="IVP39_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7779..8480)
                     /locus_tag="IVP39_001940"
     CDS             complement(7779..8480)
                     /locus_tag="IVP39_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7587005.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8588..9997)
                     /locus_tag="IVP39_001941"
     CDS             complement(8588..9997)
                     /locus_tag="IVP39_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7587006.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(10045..11910)
                     /locus_tag="IVP39_001942"
     CDS             complement(10045..11910)
                     /locus_tag="IVP39_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7587007.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(12031..12882)
                     /locus_tag="IVP39_001943"
     CDS             complement(12031..12882)
                     /locus_tag="IVP39_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7587008.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         3748 a         2814 c         2129 g         4385 t
ORIGIN      
        1 tagctcaaaa aacggcaaaa tatgaggaac tattttgccg tttgtttctc taatactcag
       61 gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc ataggattga tattgtcctt
      121 gtgatgactc attcgtaata attgaggctt gattttcttc acttcccgtt atcgtttgtc
      181 cactgccaga atctgaagga cttgttcctg tcgactcgtt tttttgagaa gagacagttg
      241 ttttcttgac ttgttcattt gtctttttcc attccgctaa acgatcggcg accacttttt
      301 tgtctacttt ttcagaataa gcggtgatca cttttccatt tttcaaacgg gctactttgt
      361 cgccatcatg agtcaaaagc aaaacggtca aatgcttgtc tttaaattga tattgtctga
      421 gagaaatcgc actaagtacg tcatcgatat taataaattg attttcttct tttccttttg
      481 gcaaataaat gtccaattcg cctttttcgt aattggcagt tattttatac tccgcccacc
      541 aataagttga gaattctttt ttgaaattat ctactgattc tttgcttgct acgatttttt
      601 ctgtttcttt ctgagcaaga tctgctgtca tttttcctga aaatgctgcg gataaaataa
      661 gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg tctttttttc ctcatgcttt
      721 cctccactcc tatttaattt tgactattct attatataca aaaaatgaaa tagaaagaca
      781 aaaaagttga aaaagatcgt aaataattga acttcttttt cacaaaatgg taagaatgga
      841 gaatttcacg catttgctct caactattct ttcttaagcc tcaaatccgt ttttatctga
      901 tacgtctttg aaccaatgac ccgatttttt gattgttctt tttccatctt gtgctaagtc
      961 tactgaaata aagccatatc gattcttata tgcattggtc cacgaccagt tatccataca
     1021 tgtccacata tgatagccta aacaattggc accttcttga atggcttgat ggacatattt
     1081 cagatgagat tggacaaatt cgatccgata atcatcgtgg atcatgccat cttctttgat
     1141 aaatcgtgct tcgttttcta cacccatacc gttttcagaa ataaagcagt cgatatttcc
     1201 ataatgatct cgtacattga tcaaaatatc atagattcct tgttcataga tctcccatcc
     1261 gcgataagga ttcattttac gaccaggcat ttcataattg tcgaaataat cctcaggcat
     1321 tggaccgttt ttagtatcga ttggtgtttc ttttgctttt acccggcgtg gctggtaata
     1381 attgatacct aaaagatcta ctgtattttc tttgataacc gtcaaatcct ctgcatggat
     1441 cacaggcatg agatcaagtt cttttactat ctcaatcaat tcacttggaa actcgccttt
     1501 tacagctgga tcaaggaatg aacgattgaa gaacgcatct gctatttgcg ctgctttaac
     1561 atcttcaggg tttgattcgt ctcttggata gcttggtgtc agattcaaga tgataccaat
     1621 aacaccacct tgctccattt catggtaaga tttgatggct aacgctgaag ccaacgcttc
     1681 atgatagcca acttggatcg cttttttcat atctgactct tctggataat gccatcgata
     1741 taaataacct gcttcgactg gaacgattgg ttcattatga gtaaaccatt tttttacttt
     1801 cgaaccaaac aattcaaaac aacttttggc atatttttca taggcttcaa ccgtttctcg
     1861 gttcaaccat ccgccttttt cttgcagata catcggcata tcgaaatgat acagattcac
     1921 aaagggttcg ataccgtttt caatcaactc atcaaaataa cgatgataga aacgaacggc
     1981 ttcttcgttg attgttccag attcatcagg tatcaaacgg ctccattgga tagatgttcg
     2041 aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc tcttgatact tcgtataaac
     2101 ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt tgcggttctt ttttgtacca
     2161 ataatcaaaa atgttctctc cttttccatc gcctgaaatc cttccttctg tttgtgggcc
     2221 acttgctgca gaaccccacc aaaaatttgc tggaaattga tattttttca tcaaagcacc
     2281 tctttttctt ttttagttcg ttttagttga ttccatgtat gatattttcc atcacaatca
     2341 ctttttcatt ttcttcctct tcttttttga ttcgttttaa cagctgttct gctgctcgtt
     2401 ccccgatttt atctgttggt ggcgtgatcg tgattttcgg aaggatactc agatcgtttt
     2461 tatctaaata atcatagtga ctgaacgcaa tatccttccc gattctgaca ccttcttgat
     2521 tgaaacattc gatggcacca atcgacatat tgtaattgct gacaaaaata gcagttgccc
     2581 ctttttctaa taatgctttt gccccgtaat agccgctttt tctagaatag tctcctgtaa
     2641 agacctctaa ttggatcgat ggatcatttt ggaccgcttc tttggctcct gctaaacgtt
     2701 ctcttgctac gtagtctgtt tgagtggcag caataaatcc gattttttta tgtccttgtt
     2761 caccatgatg agcgatcact tttgctacac tttctcgatc attcactaat attgtatcaa
     2821 tacctgctgg tccttctgga caattcaatg cgataactgg aatactcatt tttgtgagca
     2881 tttcaaatcc agaccactct tcttcggata agaagacaat cagcccatcg atcacatgct
     2941 tcatcaggta ttcaattttt tgttcaattt tttctggctg atcggcaaac ccgcttaata
     3001 ataagctata cccattttct tcagctactc tttcgatccc tgagacaact tccgacccaa
     3061 aacggctgga aatcccgttt atcaaaagtc caatagagaa gcttcgatta ttcttcaacc
     3121 ctttagcaat gatgttttct ttatagtcca acgctgaaat cgcttctgct atttttttca
     3181 tgttcttttc cttcagttgc tgaccattca agtaacggga aactgttccc actgaaacac
     3241 cagccatctt agccacatca cggatcgatg ccatacattc tcactcctgc cagttacatt
     3301 tgatttctct ttcttgttgt tcgattatga tgaccgcatt ctttgcatag tgttgcttgt
     3361 catcaatatc catataacta aagtcttcag tagagcaact tcctctcact attaattata
     3421 cttaaacaag tatttgaacc ggttcatttt taatatagca tttttttaaa agattgcaac
     3481 acttttttaa aacgctttct gaaagtggcg gattttttgc ctttttaaaa ttttattcta
     3541 aaatttactc tatttgtggg tgttttgtgc gtttttctct tccatccgtt tgaagtttta
     3601 cattccaatg gtataatttc tttatcctat caaatggggg aagtattttg aagaaattaa
     3661 tcgtactatt tattttgtct gcgggacttt tgacattgac tggctgtgat ttgtttggcg
     3721 gctctgacca atcctcggac gaaacaaccg attcttctgt cttaaaagcg gaaaaagcag
     3781 ataccaccaa cagtgtatat acgctaaaag tacgtaaaga tatggattta tccgcgggac
     3841 agttgacatt tacagataaa aaaatggaat ggacacgcac gtatcagaca caaggaactg
     3901 attcttctga atcatctgaa gaaaaaacag tgctgacaga tatcaagatc actaccaaag
     3961 atgacgaata tattatttca ggaaaagaaa ataaaaaaga aacaacagtc atatttacaa
     4021 aaatcggtaa ttaccgtttg aaggatgagg atggcaacat ctacagtcta tagtgccgtc
     4081 tgttggatct tcttttgttt ctccccttct tttctgctat agtaaagaag caagatgatt
     4141 gtaaaggaga cgaaatggat gatcgatagt cctgattatt gtacatgtaa taacaacaaa
     4201 aatatccata cagaatttaa tgaccaagga agcttgagca tatgcgacaa ctgtcacaaa
     4261 ctagtcaaag attcatttaa aacatttgaa tcttatcctg ttcaaaatta tgtaaaaagt
     4321 agaagaagat aaacaaatcc aagcaaaact cactatggct gatttttatg aacttcaaac
     4381 tcagcttctt cctttgatca agcttcatca gtaaaacaaa cagtggatca tttcagttta
     4441 caaatcaaag agtaaagatt tatttatttc aaatatttga atataaaata accaaacgat
     4501 agaaaggctt attctacatc acgtagtaat ttttctttcc cgtcgtttgg ttttttattt
     4561 ttagcctaat tcttgacctt ttgcatgctg gttatccgta aaatattttc cagcaggctt
     4621 tccttccaat tcagctaatt tttcttggat ctgacggatc accttttttg catcttctgg
     4681 attttctaca aaatcgaatt tatccccatc cacttgtatt ttaggacata catcaaaatc
     4741 ttcataccat tgattatagc gggtgttcaa cgtttcataa taggtatata acgtctcatc
     4801 gctttctagc tgttcatagt cacgcccacg ttttttgata cgttctagca tcgtgtccaa
     4861 agaaactttg atatggacca tcaaatcagg ccgttttttt ggagcaacat catctaactc
     4921 gtttagcatc gtatctaata agctatcgta ctgctgtact tcgatatccg ttacccgacc
     4981 taaatcagcg tttagatgga acaacaggct atcttcatag atggatcggt ctagcacatt
     5041 gtcatcgttc actaaagcat ttttcattgc taaaaatcgc ttgttcaaaa agaagatttg
     5101 caataaaaaa gcgtactgct caggatttga ataaaagagc ggcaatactt cgttatcttc
     5161 gacattttca taaaatggtt tagaatcaat ctcttttgca agcatatccg tcaaactgct
     5221 cttacctgca ccaatcgttc ctgaaatcaa aatcactgac atacaaccct tcctcttcta
     5281 taagtactcg cctacgtaag tttcaacagg caatgtttca aaatcatatt cacgtacagc
     5341 ttggtctgat tttaaatacg tatggagact ttgtgtaatc atcaagttcg taggtaccgt
     5401 ctcttcttgt acaatgatca tcggtttttt caacatagtt gccacaccta attcgtaagc
     5461 cgttcctgga tcgattgttt gatgctcaaa gtcaaggata gcaacaacaa attctgcatt
     5521 tgtcagttct tccatatctt tttcgtatac ttcctgcgcc catccagcgc tgaatagttc
     5581 ataattgctt tcttggtgat gacgtggaga gtagaaactt gtgactgttt tgttttcctc
     5641 caacgctttt tcgatacgac ttactcgatc gatttgctcc tctgaaaaaa atggtcctgc
     5701 taaatatatt tgactcatta ttttgcctcc ttcaataata actgattttt aatagatact
     5761 gctcgtaatt cattgaccag ttccaatgga ttgttggaaa agattacttg atccaaccat
     5821 tttttatcca taaaatcctc ttttgcaaaa gtttctaata attgttgcaa gccgctgtaa
     5881 taaccgccaa tattcaatat tccgattggc ttgtcgggcg tcagaccgat tgctatccaa
     5941 gaaagaagtt gtgagaattc ctccaatgta cctagacctc cagggaaaac gaggacagca
     6001 tctccctttt caaataataa tacctttctt tcatcgatcg tttctgtagg aataaatgta
     6061 gtaacatctt ttccaggcaa ttcagtttca aaaagcccat tcggatagat acctgtgact
     6121 tttccgttat tttccagtac tgttttacta atcactccca taatcccgga gtatgaacca
     6181 ccgtaaacca gttcgatttc ttcttgtgcc atgtattttc ctaacgtttg cgcaataaat
     6241 ttgtaacttt ctttattacc aaaacgggaa ccgcaaaaaa cagtaagttt cataaaaaaa
     6301 cactccctaa ctcaaaagtc tatcttcatt ctacacgtgt ttttttaaaa taaaagacta
     6361 gattttgtag ttttttagtg gtatatagga tagacgccac tatatatagt atttagaaaa
     6421 aaatcatttt tcagccattt tatagataaa acaaaaataa ataacgtgtt ttcatcgata
     6481 tgaaatgact tacttataga agatactggg atttcgtcct atatgccaat aacgtgtatt
     6541 attttaaaca acaaatactt gtttctttta ttaacgaaaa aatcattttg ttctcgtgtt
     6601 atgtttcact gataaatgat gtttctctaa ataaaaaagc cgacgaacta aacgatcaaa
     6661 atttgatcat ttttgttcat cagcttctcc taaaaatctt tttgttaacg ataaatagct
     6721 ggatttgtaa aaatatcact tgaatcgtca ctgctggaag aaaattcagt gataaccgca
     6781 cctgttgcgc ctgcctggaa ctagtgtttg atattgggct cgaacgtgta ctgatctcta
     6841 gcatgaagaa taatttcttt ttttactgta taataacttt ctttcctttt tggaatagta
     6901 caggaaagtt tttttgtttc ctttccttcg acataaggta taccagaccc tttcgacaac
     6961 gaaaggttct catttttcct tcatcgtcat ttcaaggagg gtgccgatgc tctggacagt
     7021 ttcgatcgct cctaaaccaa aatctgtata ttcgattctt tctaactctg attctttcat
     7081 accattcctg tctcaaggta catctattgt acttttttct tgtaatccat tctgcctctc
     7141 ctaactaatt gtcaaatcat cccaagatat gtcttcttcc tctttttctt cttggatgat
     7201 ttctgcattc ggatctactg gttttttcaa taataaaatg gttattcctg tgacaaaaga
     7261 accagctaac aatgcaccaa tgaatgccca tggtctggtc atcgtagcga ctgcaaacat
     7321 tcccgatgcc ggaatacggc tatctgcgcc ccaaaacatg cttaatccgc cacctactgt
     7381 cccaccgact cccgttgcta tgacattgcg tgccaagtcg ttcaatacga tagggaaaca
     7441 tccctctgtg atctgacaga tccccatagg aaaagcggtc ttcaatgtat cgatttcttg
     7501 acgattgagt atatttttac gaatgatttt ccctaagaaa taagcgatcg tcaagccgaa
     7561 tggggttacc atcgaagcta aaatcaatac agtgattggt tcatagattc cttctgaaaa
     7621 tcgtgccaca cccccttatc cagaataaat ggtaatccag aaacagccgt gtatctgcgg
     7681 taataagcct aaccaaacga aaaatatgag aagctatttt tgtttggctg ttcttattac
     7741 ttgatgcagg gcagcttttt cacaacctct ttttttattt atgtttcata atcaatatgg
     7801 taaggatgca gctccgtatg gtagaccgcc tccgaatatt caatcatttc cccaatttgc
     7861 gtagacgatt tccgtatccg tttgatgccg atctgtgcgt ctgttttcag aattccttgt
     7921 tcttcttctg ttaaaaatac tgccgagaaa tcatcagaaa atgagtcaat agtatatcct
     7981 tttttcgtca gcaaacgata caaagattct ttgccaaatt cttctctgct gaattgcatc
     8041 atttcttgtg gaagataatg aacaaaataa atataaggct gattgttcaa ataatataag
     8101 cgcttaaaac aaacagcttg ctcaccgaaa aaggaaaaca aaggatcttc cttctttata
     8161 tggaccaatt gcaaatccag cgtttctttc cggacttcat atcgggagtt ctccaagatt
     8221 tgcgtaaagc ttgccgcttt ggataatcga ttgtaaggac gattgcttag aaccgtcgtc
     8281 ccacgaccac ttttctttgc gacatattca tctgatgcca gcagttcgat tgcctttcgg
     8341 acagtgattt tgctgacttg aaacagttct tctaattcac tttctgtcgg caacatcgaa
     8401 ccgactggat attttcctga gaaaatatct tctttaattt tatctgcgac ttccaagtat
     8461 agtacacgtg tccttttcat aacgtcaccc tttgctttac tattactaaa agaatagcac
     8521 ataaaaaata aaaaaagaac acaaatcaca tgtgagaagt gtgttctttt tcattcagct
     8581 gctttaatta caagtttacg ccatttgagg caattacttc tttataccaa tcaaaagatt
     8641 ttttctttga tcgattcagt gtcccatttc cttcatcgtc tagatccaca tagataaagc
     8701 cgtaacgttt actcatttct cctgttgatg cactgacaag atcgatacat ccccaaggcg
     8761 tatagcccat caaatctaca ccgtctgcta ctgcattagc catcgcttcg atatgacgac
     8821 gtaaataatc gatccgataa tcatcaataa tcgtgccatc ctcttccata tgatcaatgg
     8881 ctccaagacc gttttcaaca atgaataatg gtttctggta acgattgtat aattcattca
     8941 atgcgattct cagcccttct ggatcgatct gccagcccca ttcacttgcc tcaagaaacg
     9001 gattttttac tcctcccagc aagttccctt ctgaagttgc tgcttgtgga tcagtctcat
     9061 tgattgccgt agacatataa taactaaatc cgatgtagtc cactggatat tcttttagca
     9121 actggaagtc tttttccgtt tgctctaatt tttccggatc gactccatat tttttatggg
     9181 tacgcgctgt gtatgcaggg tattctccac gtacttggac atccgtgcag aaaaaattga
     9241 attcttggtt ttggatcatt gttgctgcct gattgactgg attggcatcg atgctgtaag
     9301 ttgttgcata gataatcatg cagccgattt ggatatccga acgcaattcg tggccgattt
     9361 ttacggcttt gctacttgca acgaattgat tatgccatgc ttggaaaata ttttggtact
     9421 ctcctgctcc attgcttttt actaatcctt gacttaaagc tggaaagtga aaggccgaat
     9481 tgatttcatt gaaagtcatc caatatttga ctttactgct gtatctctct aatactgtct
     9541 gagcaaatcg ttcatagaag tcgatcagtt ttcgattttt ccagccaccg tattcttttg
     9601 ccaaatgaag aggcatttcg taatgagaaa tcgtgatcac aggttcaata tcatatttta
     9661 agcactcatc aatcagctga tcataaaatt ctaatcccgc ttcgttagga gtaccttcgt
     9721 ctccatttgg aaaaattcgt gtccaggcaa tcgaaaaacg ataacatttg aatcccattt
     9781 ccgcaaacaa ggcaagatct tctttgaaac gatcgtaatg atcgattcct cgatgattcg
     9841 ggtaaatata tttttcttga tcaatcgtcc agtcaaattc ttcactgccg ataatcgcaa
     9901 agcgctgctt acctccaggc atagcatctg caacggatag tccttttccg tctcgatcat
     9961 aagccccttc taattgattt ggcagcagtt gctccacccc ataaaaattt ttctgaaaaa
    10021 cgtgattgca tcctgatttc ctccttattt tacaattgcc agaacatctt cgtttgcaat
    10081 aatctctttt tggtctaatt caagtatatc ggcaaatgca tcagtattcg taatgacaac
    10141 tggagtgatt acgctatagc cagctgctct aattgcttcg atgtcaaaac gaatcaataa
    10201 atctcctgct ttgacgagat ccccttgttt gactaagatc tcaaagccat tcccatttaa
    10261 ttctactgta tccatcccga tatgaatcaa aatctccgct ccgtcagtcg ttgtgatccc
    10321 tacagcatgt ccagttggaa aaatagtcgt gatctctccg tctgcaggtg caactagttc
    10381 tcctacttct ggatcaatag cgataccttt tcccatcacg ccagaagaaa aaacttggtc
    10441 tggtacttca ctcaatggca gtactttacc cgttaaagga ctttttaagg tatgccgtgc
    10501 tgaagtaatc ggttctcctg cattcgcatg tttgttttct aattgattgt cttgtgtttg
    10561 ttcgtcaaat cctaaaatca atgtacctac gaatgcaaga acaaaagcaa ttcctgtagc
    10621 tattactgct actgtcacat ttgactctac accatccacc gtattaatga aagtcggaat
    10681 cgtcaataag ctgaccaaac tgctggagaa agcttttact ccggaaaatc cgataatagc
    10741 tccaccaata cctccagaaa tacaagcggc aatgaatggt ttcttcaaag gtaatgtcac
    10801 accatacaca gttggttcag tgattccaaa taacgaagtc actgtagaag aaatccctaa
    10861 tgctcgaagt ttcgtatctt ttgttcgcaa tgctacagct aaagcagccc caccttgtgc
    10921 caagatggct ggaagaagca ttggcatcaa tacatcaaac ccatattgtt cgatattcaa
    10981 aaacatgatc ggtacgaatc cccaatgcat gccgaacatc acgaatactt gccataaact
    11041 tcccatgatt agacctgcta cgattggact aaatccataa atcgagttga agagaccgcc
    11101 taaagcatta ccggcaaccg tcccgattgg tcctaaagca agaaatgtta atggaaccat
    11161 cactactaaa acgacaagcg gaactaaaat catttgcaga aattgcggaa tcactttttt
    11221 tacgaatggc tctaatttac tttgaaccca tacggctaga atgattggta tgacagaaga
    11281 agtataacca gatggtgaaa gaatcactgg gatcccaaag aaactgatag cttctcccgc
    11341 accagcaact gctgtgatag ctggatacac aagtgccata gcaattgcta ctgctaaaaa
    11401 ctggttggct ttgaactttt tagctgctgt aaaagcaagc atgacaggta agaaggtaaa
    11461 gacaccatct gctgcagcaa acaatatttg ataagtaccg ctatcagctg tcagccagcc
    11521 catcaccgtt gccaatgata agaatccttt caataccccg gcggctgcca tcgcccctag
    11581 aaaaggtgta aatatactag agataatatc gatcagacga ttcaaaatat ttttggactc
    11641 ttcttttcca tcctccgaaa ctgcaagacc ttcctcatcc acgatcgatt gataaacatc
    11701 tgctacatta ctgccaatca ctacttgata ctgaccgcca ctttgtacga cttggatcac
    11761 gtccgggtct tcttttaact tttgcgtatc tgctaaagat tcatctttta atctgaatcg
    11821 caaccgcgta gcacaatgta ctaaactttt gacattcttt tgtcctccta ccgcttccca
    11881 tacacgacga ccaactgctt gattatccat tgttttttcc ttctttcttt tttatttaaa
    11941 aaataaaaac ccagattgaa aaaaacagtc acaaacattc gtgaacctat tttttcaatc
    12001 caggttttgc ctgccataca gtaacaatcc ttatgattgg atgctttttt tctcacttac
    12061 tactctttga atatgaatcg ttaaataaat ctgctctgcg atccccattt cctgtttttg
    12121 cgtttgtcga agatactcat tgatccgttc gacacaatga aacgcttttg gatactttcc
    12181 ttgaactaat gcgtagagaa attcatcttg ctcgtcagat ccttcccctt tgagataacg
    12241 ctgggcaaaa aactgcagat gagtaatgat acgctgataa ttcaaagatt cttcatcgaa
    12301 cacgatgccg aagaatttac tgatgatact cagtatatct cttaccattg tggtagcggt
    12361 catcgtcact tgcatgtctt cgtttccttg ttggctattc acaaaatgaa aggcaataaa
    12421 accggcttca tcctcagaaa gttccacacc cgtcttttgt ttcacgaaca aaagagcctc
    12481 tttcgctgct tgatattctt tcggataaaa tttttttgtt tcaaataata atggattagg
    12541 cagttggata ttttcttttg ctcgtgtgat cgcataatga agatgatcgg tcaatgtcag
    12601 ataaatatta ggcgaaaatt caacttttag tttatcttca gccagttcga ctacttgttt
    12661 gacccattcg atctcctctg ccggaatatc agccatcagc tgctggaatt ggtttttagc
    12721 aaaagaatct cttaaaacaa attctttttg gaccgcttct ggcgaaatct cgtccccaat
    12781 ctttttttga aaagctagtc cttttcccat acaaacgact tcttccccga atccattttt
    12841 cgaaatgacg acattgttat tcaaaatttt atcaattttc attttgcttc gccctctcaa
    12901 gaaaaataaa aaaacctaga attcgcctac tgtttcttag ctgaattctg gttttgcctg
    12961 ccgtacagta acaatccatt tcttattcaa aatcatcata ccatcgaatt tttatttatg
    13021 caagcctttt catataaaaa ataaaaaaac tggaacacaa gtctatctag aaaaca
//
LOCUS       DACCSK010000078        13049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCSK010000078 DACCSK010000000
VERSION     DACCSK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.079"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IVP39_001944"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IVP39_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7587009.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IVP39_001945"
     CDS             1873..4539
                     /locus_tag="IVP39_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7587010.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IVP39_001946"
     CDS             4903..5655
                     /locus_tag="IVP39_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7587011.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IVP39_001947"
     CDS             complement(5677..6831)
                     /locus_tag="IVP39_001947"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7587012.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="IVP39_001948"
     CDS             7016..9457
                     /locus_tag="IVP39_001948"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7587013.1"
                     /translation="MKEVVMIDAARTPIGKYRGGLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="IVP39_001949"
     CDS             complement(9787..10491)
                     /locus_tag="IVP39_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587014.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10678..10923)
                     /locus_tag="IVP39_001950"
     CDS             complement(10678..10923)
                     /locus_tag="IVP39_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587015.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10940..11791)
                     /locus_tag="IVP39_001951"
     CDS             complement(10940..11791)
                     /locus_tag="IVP39_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7587016.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11957..12961
                     /locus_tag="IVP39_001952"
     CDS             11957..12961
                     /locus_tag="IVP39_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7587017.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4168 a         2315 c         2717 g         3849 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaggtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc attctttcta
    10681 ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt catgttcgtg
    10741 ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga tgatttgatc
    10801 atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca aaaagacaaa
    10861 atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt cgattggatc
    10921 cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct ttgccgacta
    10981 caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa tcaaataatt
    11041 cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg aattcgtgtt
    11101 gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt
    11161 tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct tcgaggatca
    11221 tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa
    11281 cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga tggtggggta
    11341 agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct tcaaatgctc
    11401 gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg tttggccagt
    11461 ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct tcaaaagaag
    11521 acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag aatatctctt
    11581 ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt
    11641 gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag gaaacagcga
    11701 catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg taaccatttg
    11761 atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta ttcgttcaac
    11821 tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc ttgaaatttt
    11881 tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt aaactaagac
    11941 agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata ttattgacag
    12001 agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag aatatattcg
    12061 tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa ctggacaact
    12121 agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt ttgtggaagc
    12181 aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag aagcacctag
    12241 tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat ctttattgaa
    12301 agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag ggatcgacaa
    12361 ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag atgaagcatt
    12421 cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg agttttctgg
    12481 caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac cttctttaga
    12541 agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga
    12601 agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag aagaaaaagg
    12661 tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat
    12721 ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc ctgtaaaaga
    12781 agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat
    12841 cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat ttgtgaataa
    12901 tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga atcgtttata
    12961 aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag
    13021 agaaggatcg gaaaagacga atataacaa
//
LOCUS       DACCSK010000079        12967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCSK010000079 DACCSK010000000
VERSION     DACCSK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.080"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(260..979)
                     /locus_tag="IVP39_001953"
     CDS             complement(260..979)
                     /locus_tag="IVP39_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7587018.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IVP39_001954"
     CDS             complement(1028..2674)
                     /locus_tag="IVP39_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7587019.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IVP39_001955"
     CDS             2853..3443
                     /locus_tag="IVP39_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7587020.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IVP39_001956"
     CDS             3535..5040
                     /locus_tag="IVP39_001956"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7587021.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IVP39_001957"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IVP39_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7587022.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IVP39_001958"
     CDS             complement(6476..6994)
                     /locus_tag="IVP39_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587023.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IVP39_001959"
     CDS             complement(7010..7336)
                     /locus_tag="IVP39_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7587024.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IVP39_001960"
     CDS             complement(7349..9328)
                     /locus_tag="IVP39_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7587025.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IVP39_001961"
     CDS             complement(9349..9663)
                     /locus_tag="IVP39_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7587026.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IVP39_001962"
     CDS             9831..10124
                     /locus_tag="IVP39_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7587027.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IVP39_001963"
     CDS             complement(10202..11314)
                     /locus_tag="IVP39_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7587028.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11515..11691
                     /locus_tag="IVP39_001964"
     CDS             11515..11691
                     /locus_tag="IVP39_001964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587029.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            11701..11880
                     /locus_tag="IVP39_001965"
     CDS             11701..11880
                     /locus_tag="IVP39_001965"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587030.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IVP39_001966"
     CDS             12074..12244
                     /locus_tag="IVP39_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7587031.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12967)
                     /locus_tag="IVP39_001967"
     CDS             complement(12668..>12967)
                     /locus_tag="IVP39_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7587032.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4089 a         2609 c         2204 g         4065 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 caatactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catccaattc ataccccgtt ggcgcttgag tttctaccag ctgatatgct cctggtgcta
    12961 agccatc
//
LOCUS       DACCSK010000080        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCSK010000080 DACCSK010000000
VERSION     DACCSK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.081"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IVP39_001968"
     CDS             6..305
                     /locus_tag="IVP39_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7587033.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVP39_001969"
     CDS             378..1739
                     /locus_tag="IVP39_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7587034.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVP39_001970"
     CDS             1761..2021
                     /locus_tag="IVP39_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587035.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVP39_001971"
     CDS             2035..2787
                     /locus_tag="IVP39_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7587036.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVP39_001972"
     CDS             complement(2881..3486)
                     /locus_tag="IVP39_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7587037.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVP39_001973"
     CDS             complement(3539..4513)
                     /locus_tag="IVP39_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7587038.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVP39_001974"
     CDS             complement(4891..5670)
                     /locus_tag="IVP39_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7587039.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVP39_001975"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVP39_001975"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7587040.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVP39_001976"
     CDS             7161..8558
                     /locus_tag="IVP39_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7587041.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVP39_001977"
     CDS             8639..9079
                     /locus_tag="IVP39_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7587042.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVP39_001978"
     CDS             9227..9553
                     /locus_tag="IVP39_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7587043.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVP39_001979"
     CDS             9554..10303
                     /locus_tag="IVP39_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587044.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVP39_001980"
     CDS             complement(10383..10838)
                     /locus_tag="IVP39_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7587045.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVP39_001981"
     CDS             complement(11265..11540)
                     /locus_tag="IVP39_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587046.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVP39_001982"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVP39_001982"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7587047.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACCSK010000081        12469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCSK010000081 DACCSK010000000
VERSION     DACCSK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.082"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(131..1939)
                     /gene="pepF"
                     /locus_tag="IVP39_001983"
     CDS             complement(131..1939)
                     /gene="pepF"
                     /locus_tag="IVP39_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7587048.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2115..3113)
                     /locus_tag="IVP39_001984"
     CDS             complement(2115..3113)
                     /locus_tag="IVP39_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7587049.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3339..3992)
                     /locus_tag="IVP39_001985"
     CDS             complement(3339..3992)
                     /locus_tag="IVP39_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7587050.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4324..4722)
                     /gene="spxA"
                     /locus_tag="IVP39_001986"
     CDS             complement(4324..4722)
                     /gene="spxA"
                     /locus_tag="IVP39_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7587051.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            5487..6194
                     /locus_tag="IVP39_001987"
     CDS             5487..6194
                     /locus_tag="IVP39_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7587052.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            complement(6340..9534)
                     /locus_tag="IVP39_001988"
     CDS             complement(6340..9534)
                     /locus_tag="IVP39_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7587053.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(9553..>12469)
                     /locus_tag="IVP39_001989"
     CDS             complement(9553..>12469)
                     /locus_tag="IVP39_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7587054.1"
                     /translation="PDEETSVTFSFEHQQMSYEIERKPEQVLAKRKGNGTKKQAAKVT
                     LTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGEFRNFLVSSSSDKE
                     TVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFVLVKEEEPAAGFEA
                     VLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKKSYEEKTILINKQK
                     ELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQKSSEQTKKLAMTTQ
                     NYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQDAEQLTISLDKEEL
                     NAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEKQQEIEKQKAKDTG
                     SLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARLQLFLRPGEPCLVC
                     GSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSEAHENNLQQQLSDL
                     EQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSLDRRKSKMANARIE
                     LEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQDTDSNCLEEEIRRL
                     EKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEKIEKEQERIAKKLE
                     SQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQLENIQLTETFPDTE
                     EISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQTKLDELTQLQQLS
                     QTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFELADTIGSYRGKTGL
                     EINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGVAIDALFIDEGFGS
                     LDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTEGSGQSKIRYQLG"
BASE COUNT         3426 a         2545 c         2054 g         4444 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagcctt aaaaaaatac
       61 gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa
      121 atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt tgttcaaaca
      181 tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct tttttcatta
      241 cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc tctagtgcct
      301 ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt
      361 attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg aatttaattt
      421 ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca taactatcac
      481 ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg aaatgctcga
      541 attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag taatggttga
      601 gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc gttaggatat
      661 tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg tacacataag
      721 gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg acaagcgtaa
      781 atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg tcatagcttc
      841 ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc caacgttcag
      901 agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt gcttctaaag
      961 ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag accggtgtat
     1021 aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat tccatgtatc
     1081 gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat acactttcag
     1141 gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg actttcgctt
     1201 taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt
     1261 cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta
     1321 gcaactgacc atacacgcca tgagataatt ggactatttc accattttct ccttcaatcg
     1381 ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga
     1441 tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg gctcgattat
     1501 ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct ttgaaatatt
     1561 gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa acggcttcac
     1621 taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct gtatttccag
     1681 tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg cgataaaccc
     1741 tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt
     1801 gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca aactcttggt
     1861 cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct tctcgttttg
     1921 gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta tctatcaaaa
     1981 tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca ttcatgtgtc
     2041 tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg attcctagcc
     2101 tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc
     2161 tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg ttcttttatt
     2221 tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc ttcgtacaac
     2281 attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt ttgatagatc
     2341 cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg tatggatact
     2401 gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc ttcactcact
     2461 ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat ttctttaagg
     2521 tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga tacagtctct
     2581 gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca gcaatgcact
     2641 cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg cgattgtcca
     2701 agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata attttgggtt
     2761 ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc tagattcgcc
     2821 aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa tactggttct
     2881 actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc ttttgattct
     2941 ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc acattttttc
     3001 tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt ctttcctttt
     3061 tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag catttttctc
     3121 cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc gtgaaaaagc
     3181 tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat ttttcgtttg
     3241 gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg
     3301 acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata aaatttcgtt
     3361 cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt ttctggtgta
     3421 cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac atccgcttgg
     3481 ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa taaatacaaa
     3541 tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct
     3601 tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga taattcttcc
     3661 tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc tgaatcaaca
     3721 gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat aaacagttct
     3781 aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg gaactcctct
     3841 tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt gtgattgcca
     3901 agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat tagtacacgg
     3961 atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt gccacaatgt
     4021 ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt
     4081 aagcgaattt aataaaacca gcggattttt caacctttca agctatctta tttaactata
     4141 acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc ctctctattt
     4201 ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat actttttgta
     4261 aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt tcgtattatc
     4321 cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta catcgcgcgg
     4381 taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat catcaatcat
     4441 gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt cttgtaacgg
     4501 cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg agatgatttc
     4561 ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt tcaatggttc
     4621 tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg cacgagcctt
     4681 tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc actccttttc
     4741 gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct gtttctatta
     4801 tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa aaatgaaagt
     4861 tatctaaggt taatatacag taaaaccagc attctcgctt tttttatcat ttaaaaatta
     4921 gagttttcaa atctaattca aattttctaa acatatgaaa cagcgtataa aactgtacta
     4981 ttattttatc tcaaaagcta aatgaaaccg atttcttaag attttttatg ttttttattt
     5041 ctttaatagt aatgttttct gtcattttaa ataatactgt tctttattat aacgcttatt
     5101 ttctctgtta tataagcgta gtattttaaa gcaatgatta ctaaataaaa tttactataa
     5161 acaaataaat caaataacgt tattttatgt ttgaatagtt atattgagac tgtattaaaa
     5221 aaataaatct ttcgaaaatt ccacagagat tgcattaaaa taaatcaatc gacttctacc
     5281 ataatgatta atttgttcat caaacttatt gacgtgtttc acgattttta gtgaaaaaaa
     5341 ctgaaatata aaaaccttat tctgtcaagg aaatagaaat atagtaagaa aaacctttat
     5401 ttatcagctt ttttagacaa ttttttctgt tacaatagtc aaggatttgt gaaaaaatca
     5461 atgaaaaccg aaaggcgtgt aaaattttga ttaaatcagt tgtgtttgat gttgatgata
     5521 cgatgtatga tcagcagcag ccatttcgaa acgcggtcaa aagagtcgtt ccgcttgttt
     5581 cagatgcaga tatgcatcca ctatatatcc ggttccgcca tcatagtgat gaaaacttcc
     5641 caaaagtaat ggcaggagat tggacactag aatatatgcg agcacatcgg atcagccaat
     5701 cactaaaaga tttagactac cctcatatta cggaagaaga tggcctctta tttcaaaaaa
     5761 tttacgagga agaacttgat aatatctgct tgcatgagga ggtcaaaaaa accctcgatt
     5821 tcttgaagga aaagaatgtc ccattaggaa ttatcacaaa tgggccgact gaccatcaga
     5881 caaaaaaact gaagcaactt caattgaaca attggattcc ttcccgcaat atgctcattt
     5941 ctcaagcaac aggtttccaa aaaccagaaa aagagatttt tcagctagct gagaaagaat
     6001 ttcatatgct tccagaagag accttatacg ttggcgataa ttacgacaat gatgttctag
     6061 gtgcaaaaag tgctaactgg caagcgcttt ggttcaacca tcgagaacgc aaaattgaac
     6121 agactccaat ttgcgatatc gagatttctt cttttgatca gctgttagag acaatgaaga
     6181 caatctttgt ataaccttta aaaatagaaa taaaaaaatg atagcaagga agctgggata
     6241 aaatggaatc ccagcctcct tgctattttt taaatcaaat aaacaatcgc ttctttagaa
     6301 aggctttctt acccattact gaacgctttt tcgagtctct tactcctcga tttctcctac
     6361 acttaaaatc atgcggatgt cttcttcagt cagtttcgtc aactgttctt cattaccttg
     6421 gatcactttt tggaacagtt cgcgtttttc ttgctggagt gaatccatgc gttcctcaat
     6481 cgttccttca gagatcatac gccacacttc taccacattt ttttgtccga tccgatgtgc
     6541 acgtccagca gcttgttctt caatggcagg attccaccaa aggtcataca agatgaccgt
     6601 atctgctccc gtgagattca atccagttcc cccagccttt aaggaaatca gaaaaacatc
     6661 tttttcgcca gcattaaatg cgtctaccat acttaaccga tcttgtggtt tcgtactgcc
     6721 tcttaaataa aatgtcgaga tccctagctc ggctaactct tcttgcaaga tagtcagcat
     6781 ccctgtaaat tgagaaaaca gcaaaactct tcgtttgttc tctttggcag ctaccagtag
     6841 atcttttact tgttcgagct tgccggaacc gccttggtaa tcttcaataa acagacgagg
     6901 atcgcagcaa atttgtcgca gtcttgtcag accagcaagt atcccgatcc ggtttttctt
     6961 gaatgcttca gaatccattg atgtgatttc ttcacgcatt tgacgcaagt aagctaaata
     7021 aacagttttt tgttcttctg taagtgcaga atatagattc atctctgttt tttccggcaa
     7081 gtctttcaac acagtttgtt tatctcgccg taaaataaac ggtcgaatca tttgcgcaat
     7141 ttcttctgcg gcaagttccc tgaattttgt tttagtcggg aaaaatcctg gcataatcgt
     7201 tgcaaacaat gaccacaatt cttctaaatt attctcgatt ggtgtcccac tcagtgcaaa
     7261 ccgctgaggt acagctaatt ctctcaatgc ttgtgcagtt ttcgtactgc tatttttgac
     7321 catttgtgcc tcatcaagaa tcaaataatc aattttttgc gactgataat tagctagatc
     7381 ctgtcttaag ctggcataag aagtcactcg gatatctgtg tctttagcca gtaattcttc
     7441 acgttctttt ttattcccat tgattacttg gatacttaaa gaaggggcga attttctcac
     7501 ctctgcctgc cagttgtaaa ttaagctcgc aggcgctacg atcaaagcag agaagctttt
     7561 ccgttcttct ttttccgaaa gtaaaaaggc aattgtctgt agtgttttcc caagtcccat
     7621 ttcatcggct aaaataccgc caaactgata atgacctaac atcttcaacc atcgaaaacc
     7681 ttgcttttga taatctctca tcgttgcatt caatcctttg ggaagctgtg cctggtatct
     7741 ttctggatga atgagatctt gtaccatcgt ttggaacgat tcactaaaag tagcattgct
     7801 tttttccaat tgattttgaa tgatcaatcc ttgattttta ggtacatgga tcgttccttc
     7861 ttctgtacgt atcgattccc gtaattgttg caacacttgg cttgtttgtt gaaattcttc
     7921 tgaatcaaaa gaaagaacct gaccattttc taatgtataa aaagcatcat ttctcagcaa
     7981 gctttgcagg acatgatcga tttcttgctc ctctatccct gtcacatcga atcgaatatc
     8041 gagccatgaa ccggtctcgg ttacttcgat tttcggttga tgtttatgcg catctaaata
     8101 caactctcgc aattttcttc caagtctgac ttcaccaaac tgacggaaga cagatagttc
     8161 tgtgcggaaa aaggcatata gttcttcccc gcttggcaat ggacgttcat aaccagtttc
     8221 atttttctgg tagcgataca ttctgaatag atcaagtact cgtttctctt ctactgtgtc
     8281 acgtaacacc tgaaaagcct gttctgtcga gacctcatgc ttcgggtcag tcgaatagat
     8341 gacttcgcca tagacgaaat cgactcgagc ttggattttt ccttttgctt ttcggaaaaa
     8401 gaaaataaat tcgacgggga tatctgctac ttcctcttct actgcagggg caatagctac
     8461 gtcgccgatc tctgacagca gagggataat ctctttaaac aaagacggta attctttttt
     8521 cttgaacact acttctggtt tctccaatcg gcgaatcaat tgtttccaag tttgataaac
     8581 cgtttgttga tatttcgtca gagaataaaa tgtcccttgg ataactcccc actgataatg
     8641 cataaagaaa aaatcaaaat cattcaatgc ttgcagcaaa taatcttctc caaatttttc
     8701 cactttgaat gataaaggtt tgttttgatc ggaaaattgt attccttgat cgttttgttc
     8761 atttatctgc agatgcaaac gtgtagtctg attcatctgc tctaacagga agcgggcttg
     8821 ttctactggg agaagcagat atcttttatc tagttttcct ttgacttgta agccgttcgt
     8881 acctaataat tgatgcgtct gagcacttcc tgcaagatgc gtgagtattt tttgatcttc
     8941 tttttgaaac gcccgtttat ctagacgaaa ctggtactgt ttattaacag tgtaagtttc
     9001 tttttctgtg taagcttgta aaaaatcata gatgtttttt acgatatatg tttttttcgg
     9061 tgatgccaat gaaccgatac gcaagtaaag tgccaaaata gaaagctctg ggtgatactg
     9121 attagtctca atcgtttcta cttggtattc aataatcaaa ggttgcaccg cttgaggatt
     9181 gttttgtgtt tttagacgag aaaaaccttt tgcaatgatt tctccctcac ttgttgcttt
     9241 taccggttct agcattggct ggtctttttt catgatacga gatagatttt tcgaccgtaa
     9301 ataaagttcc acagcaactg tatgcttaca atatttatgt tcctcccaat agggacattg
     9361 gcagtaatct atctcttttg ctgtaccatc caaatcaacc atatacaatt cactgcccaa
     9421 gacttctgca tgccacacgt tttcttcttg gtcagggaca accgataaca ctcggccttc
     9481 ttgcaagtat tgacgtccac gtgcaatgac tttttctgga atactccatt tcatagttac
     9541 caatctccct ttttatccaa gttgatagcg tattttgctt tgtccgctac cttctgtatc
     9601 aattcttatt tgttgtaata ctcgttcttt caattctctt acatgactaa tgatgccgat
     9661 cattcttcct tcactttcga tcgtttctaa agcttccatt gccatttcca aagcttcttc
     9721 atctaatgac ccaaatcctt catcaataaa caatgcatcg attgctacac caccagcttg
     9781 tgtctggatc acatcggcaa gagctaatgc taaagaaagt gctgcaataa aactctcacc
     9841 gccagacagg gtatgcgcac ttcgagacat acctgcctga tcatcataga tattgatttc
     9901 taatccggtt ttccctcgat aactgccaat ggtatccgct agctcaaatt gataacgatt
     9961 tttcgttaaa cgttggagat gatcatttgc tacttgtaac acttcttgaa gataaacctg
    10021 aagcacatac cgttccaaac tagtcttctt aggattttct ccgttgaatg tttgcgaaag
    10081 ctgctgtaat tgcgtaagct catctaattt cgtctggctt gtgtgataaa tcgaaagaca
    10141 ttcatcgtag attttttgat tactaatttt ttgttcttgt aaaccgtata tcttttcttg
    10201 ctttatttcg atcatacgtc ttatttcttc taactgttgg ctgatctctt ctgtatctgg
    10261 gaatgtctca gttagctgga tattttctaa ttgacgcagc cggtcagctg tcactaattg
    10321 ctcttcatga taagaagtaa ctgtacgttg gttttcttta tattgtgctt gctgttgtgc
    10381 aaatgcacag atttcttcaa ttgttgaaaa atacgactgg ctttctaact ttttagctat
    10441 tctttcttgt tccttttcta ttttttcatc tattttttct atttgttcat gttgaagatc
    10501 aagctgtgtc cgaagcgtat gaagatcttg caacaattgc actcctgctt tctgctcaac
    10561 agctagttga ttcgagtctt cttctaactg tttttcaagt cgtcgtatct cctcttctaa
    10621 acagttacta tccgtatctt gcaattgttg ttctaaactt ttgatttctc cttgcagttg
    10681 ctggagctga ttattttttt ctgtctgctg ctgtttgtag acagctaatt gttgttcgag
    10741 cttttctgct tgttgtctta tttcttccag ctctattctt gcattggcca tcttgctttt
    10801 tcgtctatct aaagattgtt cgattactac aagagttgtt tttgtttttt gttctgttat
    10861 ctcaggaaat gcctttttca aagcctgttg caagtgttct tcttgttcag ccaattgtat
    10921 attcgttgcc atgatttttt gttctaaatc tgataattgt tgttgaagat tattttcatg
    10981 tgcttcactt ttggcaatga tttgctgagt tctttctaca gcagactcag cttctgccag
    11041 ttgttgttct acttttaaga tatcttcctt atttggctgt tcgtgtttta attgttgtat
    11101 ggggtgttcc gttgatccac aaacaagaca tggctcccct ggacgcaaaa aaagctgaag
    11161 acgagcaatt tgctgctttg cccattggct tttcagctgt tctgcttgtt ctgtctgttc
    11221 tttcaatagc tgactatttt ttgttatttc ttcagttgct tttttgagtt cttctcttat
    11281 ctgtgcgagc gacccagtgt cttttgcttt ttgtttttct atttcttgct gcttttcatc
    11341 tagatatttt aactgtccaa aagcaagacg atcttcttgc agtttttcct gatccagtaa
    11401 attttcttga agaattcgag ttctttcctg atagatagcg agattttgat acagcttctc
    11461 ggcattcaat tcttctttat ccaatgaaat tgttaattgt tctgcgtctt gttttttact
    11521 acgtaaggat tcgacaattg gcaccatcat ctgtttgttt tgcagttttt gagcagcttc
    11581 tttgattttt gtttgtcggc gtacgcggtt tgcttcattt ttttgccact cttcataatt
    11641 ctgcgtagtc atcgctagtt tctttgtttg ctcactactt ttttgtgaca gttctatttt
    11701 ttcaactttt atttcttgta ggcgctggat tggttcgact aatcgttgcg taaattcata
    11761 atacccaatc catcgtttct tttcctctat ttcgtctttt tgagcagcta attccttttg
    11821 cttgtttatt aatatcgttt tttcttcata actttttttc aagctttcaa aacgatagta
    11881 ggaattttct agttgctttt gtttttcctg aagttccgtt agagcttctt tttcttccgt
    11941 gatttgtttt tcgagtattg cctgatggtt tgtccattgg gctaaaacgg cttcaaaccc
    12001 tgctgctggt tcctcttctt tgactaaaac aaaccttttt tgcagaagat gcagctcttg
    12061 ctctaaatga tccaattttt gttgctgttg ttttgctttt tcttttaatc gctcattgaa
    12121 ctgttggaaa aattgagtac cgaataaatg acgtaaaaca gtctctttat cactgcttga
    12181 tgaaaccaaa aaattacgga attctccttg gggtagtaaa acgatttgag aaaactgttt
    12241 cgcatcgaga ttcatcagtt ctctgatcaa ctgatctgct tctgtacgtt ttgtgatttg
    12301 ttttatttct tttcctttcc catcaaaaat ggtcaacgtt actttggctg cctgtttttt
    12361 tgttccgttt ccttttcgtt ttgctaaaac ttgttctggt tttcgttcga tttcatagct
    12421 catctgctga tgttcaaaag aaaaagtcac gcttgtttct tcgtctggt
//
LOCUS       DACCSK010000082        12336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCSK010000082 DACCSK010000000
VERSION     DACCSK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.083"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            124..1383
                     /locus_tag="IVP39_001990"
     CDS             124..1383
                     /locus_tag="IVP39_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7587055.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGDFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1471..2418)
                     /locus_tag="IVP39_001991"
     CDS             complement(1471..2418)
                     /locus_tag="IVP39_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7587056.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDHRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2812..4104
                     /locus_tag="IVP39_001992"
     CDS             2812..4104
                     /locus_tag="IVP39_001992"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7587057.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4119..5888
                     /gene="aspS"
                     /locus_tag="IVP39_001993"
     CDS             4119..5888
                     /gene="aspS"
                     /locus_tag="IVP39_001993"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7587058.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5997..6872
                     /locus_tag="IVP39_001994"
     CDS             5997..6872
                     /locus_tag="IVP39_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7587059.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7138..9021
                     /locus_tag="IVP39_001995"
     CDS             7138..9021
                     /locus_tag="IVP39_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7587060.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9096..9380)
                     /locus_tag="IVP39_001996"
     CDS             complement(9096..9380)
                     /locus_tag="IVP39_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7587061.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9370..10104)
                     /locus_tag="IVP39_001997"
     CDS             complement(9370..10104)
                     /locus_tag="IVP39_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7587062.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10233..10856
                     /locus_tag="IVP39_001998"
     CDS             10233..10856
                     /locus_tag="IVP39_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7587063.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            11048..12169
                     /locus_tag="IVP39_001999"
     CDS             11048..12169
                     /locus_tag="IVP39_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7587064.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12176..>12336
                     /locus_tag="IVP39_002000"
     CDS             12176..>12336
                     /locus_tag="IVP39_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7587065.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFG"
BASE COUNT         3896 a         2194 c         2655 g         3591 t
ORIGIN      
        1 ttagactgaa aaactctaac ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc
       61 agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag gaggttgctg
      121 aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa attgtatcaa
      181 aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt cgtcaaagca
      241 aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa agtattttat
      301 gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa gacacttttt
      361 ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa aaatattttt
      421 atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga taagaatctg
      481 acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag taagatggtt
      541 ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc taaagatgag
      601 gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac ttctccgatt
      661 gatcacataa aaggtgtcga aaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt
      721 gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata tgcttttgcc
      781 aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt tgaattattg
      841 aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg acatgcactt
      901 ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt gcaagatccg
      961 tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc gatttcgctg
     1021 aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat tttagattta
     1081 tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat gatcaaacgg
     1141 tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat tgcacaaggc
     1201 gggggagatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct tcatagtcta
     1261 cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga acaagattgg
     1321 acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact ttacaagaat
     1381 tagattagca taaacttatc gaaaaaagga agatcaaaca gtgctgatct tccttttttc
     1441 atattactat aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt
     1501 ttgataatct tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc
     1561 tggacgctta gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa
     1621 aacaagttgc gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc
     1681 ggttaaaatt tctctgaaac gttctccttg gaaatctgga tttctttttc gatgatctcc
     1741 ttcaccaaat tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag
     1801 aggaaaatag ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc
     1861 agcctgccga tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt
     1921 cgcttctttc aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc
     1981 ttcttttaat tcatgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa
     2041 gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga
     2101 ttgtttgaga aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc
     2161 ttttgtagcg atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat
     2221 cagttcttcg gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct
     2281 attcaaagct gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt
     2341 atgtcctcca acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc
     2401 gattcttatt tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa
     2461 aaagctgaac taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac
     2521 tggtttttag taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga
     2581 aactttacaa gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga
     2641 cactctggag atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa
     2701 agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga
     2761 tgaacaaggg tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc
     2821 caaagaccaa aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc
     2881 gaagaaacag cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt
     2941 tttgaacact atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa
     3001 gagatgtatg atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca
     3061 gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg
     3121 ttcaaaactt tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta
     3181 cgtcaattcc atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg
     3241 gagagcatgg tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta
     3301 gtcatcaatt cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac
     3361 tatcttttac cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat
     3421 ccattacgcg tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca
     3481 tctatcttag attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg
     3541 ctggatgcat tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat
     3601 tacacacata cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg
     3661 atttgcgcag gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct
     3721 ggcttcggat ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta
     3781 gttatccctg catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat
     3841 atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac
     3901 ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta
     3961 gtattggtga tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca
     4021 agccgaaaag aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat
     4081 gatgaaatga ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt
     4141 gcggattagt ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa
     4201 aaagacgaga ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc
     4261 aaatcgtctt taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca
     4321 gtgaatacgt gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc
     4381 ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag
     4441 caaaaacccc gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga
     4501 aatatcgtta tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg
     4561 cgacaacaaa agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac
     4621 cttacctagg gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc
     4681 atgctggaca tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga
     4741 atgctggatt cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg
     4801 gagaccgtca gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag
     4861 aaatccaaac ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg
     4921 aagtcacatt gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg
     4981 acaaaccgga tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag
     5041 tagaattcaa agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg
     5101 ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag
     5161 gacaattcgg agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc
     5221 caattgctaa attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac
     5281 caggtgactt gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg
     5341 ctatccggac acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct
     5401 tatgggtaac tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg
     5461 ctcaccatcc attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg
     5521 caaaagtcta tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt
     5581 ctatccgtat tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa
     5641 aagaacaaat ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc
     5701 cacatggcgg tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata
     5761 atattcgtga agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg
     5821 caccaagtat ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag
     5881 aagaatagcc gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg
     5941 atttaattga aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga
     6001 aaaaaacatt cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg
     6061 tttatgcgtt gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt
     6121 caagtggtat tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat
     6181 tcgaagtacc ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt
     6241 ggttaaaaat cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc
     6301 tttgtatcca tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt
     6361 ttgggggggc tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg
     6421 gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta
     6481 tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat
     6541 atatgtttta cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata
     6601 ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg
     6661 aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata
     6721 agcatgataa gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta
     6781 gagaagcaat gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga
     6841 tacttggtcg tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata
     6901 ctgctgtaga cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa
     6961 gaaaaactgc ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt
     7021 taaatttatg ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt
     7081 tcacttaata cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg
     7141 gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca
     7201 ttagctgtgg tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat
     7261 ttgggcgttc cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag
     7321 gaatgggaac aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga
     7381 ctctatccgg cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag
     7441 cgagggctgt taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac
     7501 tggtggcaaa tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt
     7561 acgcatatct ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt
     7621 gtattgttga tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca
     7681 tccttcctct ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt
     7741 gtttattacg gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt
     7801 gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat
     7861 atcgctggag gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat
     7921 tacaatttca tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt
     7981 gcagtgacag cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact
     8041 tggattggca aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat
     8101 gaagcaaagg tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca
     8161 ttgattctaa tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt
     8221 gattatggtc gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag
     8281 agcaaaccat gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt
     8341 acggcaccga aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa
     8401 agtaaaaaat tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac
     8461 caagcgatcc ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta
     8521 gaactaacgg catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt
     8581 tatccaaaag tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac
     8641 attgaaaaat taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg
     8701 aatggctcat tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa
     8761 gtatactggg tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg
     8821 cttgaacaga tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt
     8881 aatgaccata gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca
     8941 cattacattc acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt
     9001 gagacaaacg tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc
     9061 ctgaagtttt tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg
     9121 gttgagtgtg tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct
     9181 tctgcttttg ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt
     9241 cttctttttt gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt
     9301 ctagaaagat cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa
     9361 aaataatgtt catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat
     9421 attgtaagtg atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc
     9481 ttcaactaac aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt
     9541 aggtgcaatt cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg
     9601 gaccatagta aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc
     9661 taacgtcgta tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt
     9721 tgttggcagc cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg
     9781 aacgacagat aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg
     9841 gttaagcgcg atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc
     9901 gatagccgcc tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag
     9961 gtcaacaatc ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa
    10021 agaaaaagaa aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc
    10081 gattcgttcg tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta
    10141 ttatactatt tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat
    10201 actatgacca taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag
    10261 ttcgagtact attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac
    10321 cgcaagatga gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc
    10381 tggcggtagc tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa
    10441 atccagttct gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc
    10501 caggaccaag cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga
    10561 tcatgtttcc cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt
    10621 ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg
    10681 atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata
    10741 tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg
    10801 acaaattgga caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg
    10861 ataagtttat gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca
    10921 atgaaaacga gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag
    10981 aagacaggtg tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg
    11041 tgattgaatg cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta
    11101 tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga
    11161 aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga
    11221 tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat
    11281 ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt
    11341 cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt
    11401 taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt
    11461 tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca
    11521 ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag
    11581 tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga
    11641 actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag
    11701 acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca
    11761 aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat
    11821 cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc
    11881 cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat
    11941 catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg
    12001 ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc
    12061 tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca
    12121 agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa
    12181 accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc
    12241 acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac
    12301 gatttttgat gggatcacct tcgcactttt tggtga
//
LOCUS       DACCSK010000083        12237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCSK010000083 DACCSK010000000
VERSION     DACCSK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.084"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(78..335)
                     /locus_tag="IVP39_002001"
     CDS             complement(78..335)
                     /locus_tag="IVP39_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587066.1"
                     /translation="MEQILTAASIITPLVIAVTGLIKTQVNNYKILPVINVIAGIFLG
                     VLYALSFLQDAVVLYAWAGAVSGLAAGGLFDLGVSVIKKEK"
     gene            complement(338..571)
                     /locus_tag="IVP39_002002"
     CDS             complement(338..571)
                     /locus_tag="IVP39_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587067.1"
                     /translation="MSAVSITIAEWIAILTLASTVIFGVAKFYAVFTDLRKTLDRLNG
                     TIQGLQESQSEYGNRLVAIEEQIKTLFKQIGGE"
     gene            complement(617..1426)
                     /locus_tag="IVP39_002003"
     CDS             complement(617..1426)
                     /locus_tag="IVP39_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HAP7587068.1"
                     /translation="MKKFVKLASLSISIILFSACNLTTGFNWLNNKETILSAHRGAHT
                     AAPENTIEALEAAIDMNYGAIEIDPRMSSDGNMFLMHDDTVDRTTDGAGPLEKMTSEQ
                     IENLKIDTTDYPEFSNKTVKVPTFDEAVKVISKSDLILNVDGSKGDWLNKEFVDNIVH
                     TVKSHDMFEKTFFVLSDKKEREFFNKLYPEACVSWLFDSNSTIEKEIEKAKNYKHAML
                     SVSNDFATKEVIEKLNKSKIYYQVYSVNTQERFEELKSWYAKMIETDTLKP"
     gene            complement(2028..2342)
                     /locus_tag="IVP39_002004"
     CDS             complement(2028..2342)
                     /locus_tag="IVP39_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587069.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VFSQTIFYGGIISISLLSSTLYSLNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NK"
     gene            2628..2867
                     /locus_tag="IVP39_002005"
     CDS             2628..2867
                     /locus_tag="IVP39_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587070.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(3230..3490)
                     /locus_tag="IVP39_002006"
     CDS             complement(3230..3490)
                     /locus_tag="IVP39_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587071.1"
                     /translation="MNNETIILGRKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(3676..4173)
                     /locus_tag="IVP39_002007"
     CDS             complement(3676..4173)
                     /locus_tag="IVP39_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7587072.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(4303..5013)
                     /gene="budA"
                     /locus_tag="IVP39_002008"
     CDS             complement(4303..5013)
                     /gene="budA"
                     /locus_tag="IVP39_002008"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7587073.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(5026..6690)
                     /gene="alsS"
                     /locus_tag="IVP39_002009"
     CDS             complement(5026..6690)
                     /gene="alsS"
                     /locus_tag="IVP39_002009"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7587074.1"
                     /translation="MAKKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(6902..7498)
                     /locus_tag="IVP39_002010"
     CDS             complement(6902..7498)
                     /locus_tag="IVP39_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587075.1"
                     /translation="MKIKLYLLSAFFLVFSAGLFFLPDILASSITAKEQRTVDISSLS
                     SASSPSIETAMSQAQSFSIMEESDQSQLDMVNRLREELNQLFQKLSMPKEPVIVQDYE
                     VLISEDSVFWQMEFLTQTGYGTAIIDETNKKVVRLSYFDEDQNNRGKKQIIDDYLNYL
                     PLKADKIEEDGEMITVTLKNGQKLNFLIDETRLLLNYI"
     gene            complement(7488..8870)
                     /locus_tag="IVP39_002011"
     CDS             complement(7488..8870)
                     /locus_tag="IVP39_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7587076.1"
                     /translation="MKYTSKVFFSLLFFFLLIYLVVSGFTLHAIYQRTVSQEKEVLVN
                     QQKNLLLLLSTKTDGDETTDEMRKNGEFVAAAKIGEFEILDKKGKTVFSTKPTLFEKY
                     DSPKELSNHIVEISDMVTVKNKKILYSTSKIPDTTYRLATYHQAKNYEEIAKDSLVYF
                     RLILLFLLLAIIPVTYFLTSMILKPINQLISVAQHITEGKTKERAIVNTQDEIGKLGN
                     SLNNMADKLNYDLETLRQAKENQDLFVSSFSHEIRTPLTSIIGYAQMLKWEKLDQSAE
                     ESVDYILEESNRLKHLSNDILKLIELERTNVSLEEISTSMISNDLNKFLKGMKQGFTY
                     TIELEPANILLDINLFKLLAMNILANSLEAIAEDGKLVVKGIKKEKRYILQFSDNGRG
                     IPAENLSKVTDEFFTGNPSRTTKHLGLGLALVKKITELHNSSFDIESTLDSGTMITID
                     FALGGETDEN"
     gene            complement(8873..9529)
                     /locus_tag="IVP39_002012"
     CDS             complement(8873..9529)
                     /locus_tag="IVP39_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7587077.1"
                     /translation="MKILVVEDNLAINKLLVRTLEKNGYTVYSVFTGKEAADIIEQQE
                     FDLALLDIMLPEIDGYEVFAYMREYDLPVIFLTAKDQISDRVSGLRSGAEDYITKPFD
                     LEELLARVEVVLRRNGKSSSNKLLKWKYIEINEKTRAVTARNQAVSLTPKEYELLLYL
                     IRNKAIILPREKIYQKIWGREEEPNSRTLDLHIQRIRNKLQLGQDLRTIYGVGYLLEG
                     "
     gene            complement(9504..10202)
                     /locus_tag="IVP39_002013"
     CDS             complement(9504..10202)
                     /locus_tag="IVP39_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HAP7587078.1"
                     /translation="MIQKQMYFIRGYYILLLLVLYLSFYEKSVFYLFFTNTILAYIPI
                     ELAMWIKSSRSKLVSLVLILFWLLFFPNNFYLFTDLFHLSLLSPYDPSTMMISMEMEI
                     WYKLLLVVLSVFPTTFLGCESLWIVTDIIVNKIGIGVYQWLVVSIILFINAIGIYFGR
                     FLRLNSTDVFTDSKETLMRLIEAFTRIDSPSFLLIVLIFFFSLIFVYIYKLGIKFALL
                     AEGEDLYEDTSSRR"
     gene            complement(10225..11078)
                     /locus_tag="IVP39_002014"
                     /pseudo
     CDS             complement(10225..11078)
                     /locus_tag="IVP39_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(11095..11985)
                     /locus_tag="IVP39_002015"
     CDS             complement(11095..11985)
                     /locus_tag="IVP39_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7587079.1"
                     /translation="MYKTLKIISIFLLMFIIAAGAYGVSEYMKLQNLSEVSKKTAANI
                     NVKYSADTDTQLPDRFNVLLLGVDTGEFGRTERGRSDSMIAAHVDLKNEAIKLISLER
                     DTYVQIAGQNRFDKLNAAYAYGGEQTAIQTTESMLDTTIPYYMAMNMKGLESILSIVG
                     DIEVVNDFAFSFDHYDFPKGSLTLSPEEVLAWSRMRYDDPNGDYGRQIRQQKVIKAIM
                     QKLLTLESIPKVDKIINVLGNNMKTNLPLGELLKLYITKNNKFKMSTDQLKGEEEIID
                     GISYQKMTDAEIKRISQQLQ"
     gene            complement(12148..>12237)
                     /locus_tag="IVP39_002016"
     CDS             complement(12148..>12237)
                     /locus_tag="IVP39_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7587080.1"
                     /translation="YRITTVGDLLGHPTAVENYYGRNDHRPVQ"
BASE COUNT         3630 a         2418 c         1943 g         4246 t
ORIGIN      
        1 ctcttaaatc ttgaatcgta gtcataaata gccctcctaa ttttatttat agaaaaagag
       61 cagtcaagcg actgctctca cttttctttc ttgatgacac tgacacctaa atcgaataac
      121 ccaccagcgg caagccctga aacggctcct gcccatgcat ataaaacgac tgcatcttgc
      181 aggaaagata atgcgtataa gacaccaaga aaaataccag caatcacatt gatcactggc
      241 aagattttgt aattgttcac ctgcgttttg attaaccccg tcaccgcgat cactaacggt
      301 gtgatgattg atgctgcagt taaaatttgt tccatcttta ttctcctccg atttgtttaa
      361 atagtgtttt gatttgttct tcaattgcaa ccagtcgatt gccatactcg ctttgacttt
      421 cttgcagacc ttggatggta ccattcagcc gatccaacgt ttttctcaaa tcagtaaaaa
      481 ccgcatagaa ttttgccacg ccaaaaataa ctgtactagc aagcgtaaga atagcgatcc
      541 attctgcaat tgttatagat accgcactca tttgccacct ccaactagaa aataaaaaac
      601 cactaaaaat agtggtttaa ggctttaatg tatctgtttc aatcattttt gcataccaag
      661 atttcaattc ttcaaatctt tcctgtgtat ttacagaata tacttggtaa tagattttgg
      721 atttattcaa cttttctatc acttcttttg tcgcaaaatc atttgaaaca gaaagcatag
      781 catgtttgta attttttgct ttttcaattt ctttctcaat tgtactgttt gaatcgaata
      841 gccaagagac gcacgcttct ggatataact tgttgaaaaa ttctcgttct ttcttatcgc
      901 ttaagacaaa aaatgttttt tcaaacatat catgtgattt cactgtatgg acgatattat
      961 ccacaaattc tttattcagc cagtctcctt ttgatccatc cacattcaag attaaatcac
     1021 ttttagaaat aacttttact gcttcatcga aagtaggaac ttttactgtt ttatttgaga
     1081 attcaggata atcagtagta tcaattttaa gattttcaat ttgttccgat gtcatttttt
     1141 ctaaaggacc tgcgccgtca gttgttcggt ctactgtgtc atcatgcatg agaaacatgt
     1201 ttccatcgct actcatgcgc ggatcgattt caatggctcc gtaattcata tcgattgctg
     1261 cttcaagcgc ttcaatagta ttttctggtg ccgctgtatg ggctcctctg tgtgccgata
     1321 aaatagtttc tttgttgttt aaccaattaa accctgtggt taaattacaa gcagaaaata
     1381 aaataatgct tatactcaaa ctagctaatt taacaaactt tttcactaat attctcctta
     1441 actcattatt aacattagta tagcaaagac ttttcataca aaacagttaa ttcttatttt
     1501 ggaaaaggat cacctgtata ccaacgtccg taaaaatacg catttccaga aacattgcac
     1561 ccctttctta atagatttgt ttgcatccct tctagattcc tgccgtctcc attgtgagat
     1621 ttcagcagat ttcgctagat gcttaaatcc ttattttata agggtttgag cattttttat
     1681 ttttgctgat tttcgttaaa tgaatgaaaa tggcattcaa aaaaacacat tttttaatat
     1741 aaattacttc ctccatatta tcaacgctat tcctacttca caaaaatact agttcgtata
     1801 tcctttttga ggtgaacatt aagttaatga aaggtaaaat atagttatgg tttaagtatg
     1861 ttccttcaaa taagtttttc aattataaac tctagagaaa ttaattcatc ctaacataaa
     1921 ataattgttc actcaaaagt ttttctagaa caattaaaac agcaggcttc acattcgcta
     1981 tacatttgta agaagcaata attcttgtac taaaaggcat aattgcttta tttatttttt
     2041 gacttctctt caaacacaag aagtagttgt gtaataattg cagaaataag gattccaata
     2101 atagatataa aatttgacat ggaaacgtct ataacattta aagaataaag tgttgaagaa
     2161 agtaaactga ttgaaataat ccctccgtaa aaaatagtct gtgaaaatac ccactttttt
     2221 tcagaagacg tacttcgttt ggttctaaaa cctatccctg catttctact cttatcaaaa
     2281 aaccaaatat ttagaagtaa taatattgct attattaaga taaaattact gtaaaaatcc
     2341 aaaagctatc ctcctagcaa ctatttaaat gcccaaaaaa attacgtcta gataatatca
     2401 tacctacgtt ttcacttata aattttttca taaaaaaagc gtttagaagc ataatcgtta
     2461 atacagatgg taaccaatgt aactggtttg aactgggata tcaccctctc catatatgta
     2521 ttgctaagac taatagatac tataaaagac ttgatagttt tgcattgatt ttgcctaata
     2581 ctgagcatag gtgtatgcta aacgatacta taaggagtgt gtacataatg aaaagaattc
     2641 tgtttatcag tttatcaaat ttattgttac ttactgcttg tgggacttct gcatcaaaga
     2701 acataccttc agatacgcaa atttcgaata gtatcgattc aacaatttct acaaatgaga
     2761 aaaattctcc ggtagaaact tccgatataa aagtttctac ctctgcaaaa aacagggtaa
     2821 agaaactgtc agcacgtctc ctttatttca aacaaactct gaattagctt cttcatcttc
     2881 ttcagcaaat aatgatgata gtttcgcgtt tactcctaac taaatgcaac cttttacaaa
     2941 tgctttacga taacaagcct gagctaaatg atcctgatat cagttttgca ttttttgaaa
     3001 tgattggatc ggatgatctt tttaaaacta ctagtttgtc tatccgaaaa taggacagtt
     3061 ctggcagtat aggtttttat cgagtttctc caccaggcct cgttacagaa acagattctc
     3121 taggcgaacc ttttgaataa atcaaaaatc atgaacaatc actgccaact aaataaagtg
     3181 ccttcaaaag cttggtcttc tcgcttttga aggcacttta tttaaatctt tatgaaacta
     3241 aagtatgttc atatttgttg ctgtagttat atagatattc tgttgtttcc aactcataaa
     3301 cgtgagtata ttttgccact acttttccgc atttttctaa tatttcattg cgatcgacgt
     3361 catgatattc gtctacgcag atcacaacac aattttctaa tttactgatt acttctccaa
     3421 ccactttttg tttcaagcca ataggttggc aaatatattt tcttcccaaa atgatagttt
     3481 cgttattcat atttcatcca tccttaatgt ttttttcatt attcaaaatg ataataccat
     3541 taaaatatga ttatgaaaac caataacact tgtgatttta taaaaaaata aaaaaaccga
     3601 aaaacagaaa tgcccactgt ctttccggct ctttttattc ttgatttctc ttcttttttg
     3661 tttcaataga cgattttagt tattaataaa tgaaaaagca tttatattaa aatttgctaa
     3721 taaaagatct acatattttt ctagaaatgc ttgatcgatt tcatcatacc ctttcgttac
     3781 tccgcaatcg atatctaaaa caccgattaa attaccttct tttaccattg gcactacgat
     3841 ttctgattgt gcggctgaat cgcaggaaat gtaatttgca tgggttttga catcatcaac
     3901 gatcaatgtt tcttgttttt cagctgactc tccacaaact ccttttccca tcttgatacg
     3961 tgtgcaagaa actcgccctt ggaacggtcc taaaatcaat tcattaccat cgaataaata
     4021 atatcctgca aagacagtct caggcaaagt ctcattcaac aatgcagatg aatttgctaa
     4081 atttgctaac cagttatctt ctgcttctaa taatccttgt tgctgtaata ataaaatttc
     4141 gtatgcatct tttttctgtt tttctgtcca catgataaaa accgctcctt tttgatctat
     4201 ggagaagatt atagctcagt ttttttgtaa acgctaggtt tcctcctcaa aaaaacgaga
     4261 tacgtcaggt ttatcacctt gaacgcatct cgctgtcttt ttctattctg cttgttggat
     4321 ttctgctgct aaatcagcat aatcaatcgt ttcgtgaaca aaagtcggat catcaactgg
     4381 aaagtgctgt acaagggacg agatgcggct aatctcaact tttccttcag ccacttcaaa
     4441 atccattaca tgtccgccaa atgtatgtgt atcatcgata aaatgcaggt gaaatcccgc
     4501 tgcagcaact ccttggaata attcaggcgt aaagaacccg actaatgttc cttggacatt
     4561 gctttgttgg aattctggtt gagactctga aatttttgct agtcttctgt aaggtttttc
     4621 ttgcttcggc atgatacgga tgtgcatatt tttgaataat ccagttatct tcactgccaa
     4681 aaataggttt tccccggctt ctcttactaa tatgtctttt ttcaattgtt ctccatctgc
     4741 agtttttgag acagaaaaac tagtatctgg cgtaaaatct gtaattgctg catagggggt
     4801 aagttcactt ccatctaatt gggcaactga gccatctgat cttccttgat aagctacgcc
     4861 atctaaaaag atgacttcac catcaagtcc atgcaatgtt cccaatccca gagttccttt
     4921 ttctaatata tgagcaatcg tttctgttcc atccatcaag ccggccatca aagctcctaa
     4981 tgtcccgtgc tggtataaga ttttttcagt catgtcgaca cttccttaat agaattgatc
     5041 tggtaagatt gtttctccca atttctcatt atcacggtaa tcaatcggaa tatcaataat
     5101 tacgggtcct tctgtcgcaa atccttcttc taaagctttt tctaattcct ctacacttgt
     5161 agctcggatt cctttagcgc caaaagcttc tgcatatttc acaaaatcaa ctggaccaaa
     5221 gtctacacct gacgcccgtt gatatttcat tttttcttgg aactctacca tattgtagtg
     5281 tccatcattc caaatcaaat gaacgatatt caatttttta cgtacagccg tctctaaatc
     5341 ctgggcagag aataaaaatc ccccatcgcc ggaaactgat acgatttgtg tatttggacg
     5401 caccaatgca gcagaaatcg cccatggcaa tgcaacacca agcgtttgca taccattact
     5461 gaataacaaa tgacgaggtt cataacttct aaagtggcgt gccatccaaa tataatgact
     5521 acctacatct acggtaacag tcatatcatc tgttactttt gactgaagcg tgttaatgac
     5581 ttctaaagga tgcaagatac ctgcttctcc ttttgattga acaatgtccc gctccgttaa
     5641 ttttgcttga agagatgcta aatattcttt tgcatcttct gacacttgtt gaggctctaa
     5701 tgaacctgtc aataaatcaa gagtggctga gatatcaccg atcaattctc tttcaggctg
     5761 cataaaagga tcgatttcag ctggcgcttc atcgatcaca atgatccgcg catctttttc
     5821 cgcattccag tttcttgctt catattcgat tggatcatag ccaattgcaa taactagatc
     5881 acttcgtttc aaaagcatat ctcctggttg gtttctaaat aaacccacac gaccaaagaa
     5941 atgcgcttct aattcacgtg agatcactcc agctgcttgg aatgtctcta caacaggcaa
     6001 tgctgtcttt tcaactaatt gcctgatggc taacgtttct ttttcacttg atcctcgcat
     6061 acctactaat agtaccggta gttttgcttc tctaatgcgt tcagctaggt aacggatatc
     6121 atctgcagaa gcagaaccta atttaggatc gctcaatggc ttgatgacat taccttggac
     6181 aggtgcatcc actacatctt gcgggatact gataaagctc gcgccttttt ttgaactctt
     6241 agccatacga taagcattgg caatcacttc tgaaattgtt tctggatctt ggatttctgc
     6301 actgtatttt gtgattggct ggaacagtgc tgcattatcc atgctttgat gggtcaactt
     6361 caatagatcg cttcgtttta cttgtccggc aattgctaaa actgggtcac cttctgctgt
     6421 cgctgttacc aaacctgtag ctaagttact agcaccagga ccacttgttg cgataactac
     6481 tcctggctct cctgtaatac gtccgattgc ttgtgccata aatgcagcat tttgttcgtg
     6541 acgagtcacg attaattcag gaccttgatc ctctaattca ttaaatacac catcgatttt
     6601 tgcacctggt ataccgaata cataatcaac gtgatgatta atcaaacttt ctactacaga
     6661 tttcgaacct tgtttcattt ttttagccat tgaacatcgt cctttttatg gtttattttt
     6721 aattattagt tagaaataat cacaaataag tgatacagtt tctaaacctg tttcattcag
     6781 aagtatagca cgttttattt catttgttta gccatatgtt tcaaaaaatg aaaaagaatg
     6841 acagttttta cataaaaaac tgtcattctt aattgaaagt ttcaaccaat ttcacaaaag
     6901 gtcatatata attcagcaac aatcgtgtct catcaatcag aaagtttaat ttctgtccat
     6961 ttttcaaagt gacagtaatc atctcaccat cttcttcgat tttatctgct tttagaggaa
     7021 gatagttcaa gtaatcatct ataatttgtt ttttccctcg gttattttga tcttcatcaa
     7081 aataagataa ccgtacaact tttttgttcg tttcatcaat gattgccgtt ccgtagcctg
     7141 tttgcgtgag gaattccatt tgccaaaata cgctgtcctc tgagatcaaa acttcatagt
     7201 cttgaacaat tactggctct tttggcatgg atagcttttg aaatagctga tttaattcct
     7261 ctcgcagccg attgaccata tctaattgac tctgatctga ttcttccata atagaaaaag
     7321 attgggcttg gctcattgct gtttcgatag agggagaaga agctgaactt aaagaagaga
     7381 tatctactgt tctttgttct tttgcagtaa tactactcgc taaaatatct ggtaaaaaaa
     7441 acaatcctgc gctgaatacc agaaagaaag cagaaagaag atataattta attttcatct
     7501 gtttcccctc ctaaagcaaa atcgatcgtg atcattgtcc ctgagtctag cgtactttct
     7561 atgtcaaaag aactattatg caattcggtt attttcttta ccaaagctaa tccaagtcct
     7621 aaatgtttcg ttgtccttga aggatttcct gtaaaaaatt catctgttac tttagacaaa
     7681 ttttctgctg gtatccctct cccattatcg gaaaactgca gaatataacg cttttctttc
     7741 tttattcctt tgaccactaa ttttccatct tctgctatag cttctaatga attcgccaga
     7801 atattcattg ctagtaactt gaaaagattg atgtctagca aaatattggc aggttctagt
     7861 tcgatggtat aagtaaatcc ttgtttcatt cctttaagaa acttgtttag atcattgctg
     7921 atcatgctgg tcgatatttc ttcaagagag acatttgttc tttccagttc aatcaatttc
     7981 aaaatatcat tggacaaatg ttttagccgg ttgctttctt caaggatata atcgacagat
     8041 tcttcggctg attggtccaa tttttcccat ttcagcattt gagcatagcc aataatggaa
     8101 gtcagcggcg ttcgaatctc gtgagaaaag ctagaaacaa ataaatcttg attttccttt
     8161 gcctgacgga gagtttctaa atcgtagttc aatttatcag ccatattgtt caaactgttc
     8221 cctaatttcc caatttcgtc ttgagtattg actattgccc gttctttcgt cttaccttct
     8281 gtgatatgct gggctacaga aatcagctga ttgatgggct tcaagatcat gcttgttaga
     8341 aaataagtga cgggaataat agccaaaagc aaaaagagta aaatcagccg aaaatagaca
     8401 agggaatctt tcgcgatttc ttcatagttc tttgcttgat ggtaagtagc taaacgatag
     8461 gtagtgtcgg gtatctttga agtcgaataa agtatctttt tgtttttaac agtaaccata
     8521 tcactgattt ctacaatatg attagacaac tcttttggag aatcgtattt ttcaaacaaa
     8581 gtaggctttg tactaaaaac cgttttacct tttttgtcta gaatctcaaa ttctccgatt
     8641 ttggctgcag ctacgaattc accatttttt ctcatttcat ctgttgtttc atctccgtct
     8701 gttttcgtcg acaaaagcag taagagattt ttctgctggt tgactagcac ttctttttct
     8761 tgagaaactg tgcgctggta aatcgcatgc agtgtaaaac cactgaccac caaataaatg
     8821 agtaaaaaga agaataataa tgaaaagaaa actttgctcg tatatttcaa gttcacccct
     8881 ccaataggta acctacacca taaatcgtac gcaagtcctg tcctaattgc aatttatttc
     8941 tgattcgttg gatgtgtaaa tcaagcgttc tggagttggg ttcttcttcc cttccccaga
     9001 ttttttgata gattttttct cttggcaaaa taattgcttt atttctaatt aagtaaagaa
     9061 gtaattcata ttctttagga gtcaaagaaa cagcttgatt tcttgcagtg acggctcttg
     9121 ttttttcatt gatttcgata tacttccatt tcaataattt atttgagcta gattttccgt
     9181 ttcttcgaag aacaacttcc acccgtgcca gcaattcttc tagatcaaat ggctttgtta
     9241 tataatcttc agctcctgac cttaatccac tcactcggtc ggaaatctgg tctttggctg
     9301 tcaagaaaat aacggggaga tcatactcac gcatatacgc aaatacttca taaccatcga
     9361 tttcaggaag catgatatcc aacaacgcca aatcaaattc ctgctgctcg atgatatctg
     9421 ccgcttcctt tcctgtgaat actgaataga ccgtatagcc atttttttct aatgtacgaa
     9481 ctaaaagttt attgattgca agattatctt ctactactag tatcttcata cagatcctca
     9541 ccttctgcca atagagcaaa ttttatccct aacttataaa tatacacgaa tatcagtgaa
     9601 aagaaaaaga tgagtacgat caataaaaaa gatggactgt ctatcctggt aaatgcttcg
     9661 atcaatctca tgagcgtttc tttgctgtcg gtgaagacgt ctgtactgtt caatcttaaa
     9721 aagcgaccga agtaaatccc tatggcgttg ataaacaaga tgatgctgac aactaaccat
     9781 tgatagacgc ctattccgat tttgttgacg attatatcgg taactatcca taagctttca
     9841 caacccaaaa atgtggttgg aaagacagaa agaacgacta gcaacaattt ataccagatt
     9901 tccatctcca tacttatcat catagttgat ggatcataag gtgaaagcaa agacagatgg
     9961 aataaatcgg taaacaaata aaagttattg ggaaaaaaca gtaaccagaa taagatcagc
    10021 acgagagaga ctaatttgga tctgctggat tttatccaca tcgctaattc aatcgggata
    10081 tatgccagaa tcgtattagt aaaaaataaa taaaaaacac ttttttcata aaaagaaaga
    10141 taaagaacga gcagcaacaa aatgtaatat cctctaataa aatacatctg cttttgaatc
    10201 attggacatc tctccttttt taaactaaac gggtcggtga tcattgcgcc cataataatt
    10261 ttctgctttt gatggattat ctaataaatc gctgatggtc gtaaaaaagt atatccttct
    10321 ctttttaatt catcaatcag cacaggaata gcatctacgg tctcaggata gatatcatgt
    10381 agtaagataa ttgcaccgtc atatactgtg ctttttactt gctgcagaat tttttccttt
    10441 tgtctagatt gccaatcttt cgagtcaaca gaccattcga tcattgggcg attaatgatt
    10501 cctgctcctt tttttgtgat agagccatac ggaggtcgga catatgctgg ctttttaccg
    10561 attgctttca aaatagcgtt atctgtttga gtaatttcag cactgatttc ctggtcagat
    10621 agacctgcta aatctttatg tgaaaatgta tggctgccga tttgatgacc atcttgtacc
    10681 tcttttttca aaagttgttc gttctgggaa acattttctc ctagaacaaa aaaagtggca
    10741 tgtatatctt tttgttttaa aacttctaaa agtttcggcg ttgtggacgg attaggcccg
    10801 tcatcaaagg taagagcaat ggtctttgat gatttcattg tatcttccgt ttctttattt
    10861 ttttcatttt ttgtttgctg ttcctttgtg gtgatcgttg gactggtaga attttgtatt
    10921 tctgtttgct cttgtttcct ggagctttca gtattttcta ttgttttgtc ttcgttcgtt
    10981 gagggcgtga agactctgtc tgccatatag acgcttcccc ctgtgatccc tattagaagg
    11041 atgattattc caaaggctat caaacttttt ttaatcatat tcctctctcc tgctttactg
    11101 taattgctga ctgattcgtt tgatttctgc atcagtcatt ttttggtaag atatgccgtc
    11161 aattatttct tcttcccctt ttaattgatc cgtactcatt ttaaatttgt tgttcttcgt
    11221 tatatataat ttcagcaatt ctcccaaagg taaattggtt ttcatattgt tgcctaaaac
    11281 attgatgatt ttgtctactt taggtatgga ttccagagtc aatagcttct gcataatcgc
    11341 tttgatcact ttttgctgtc gaatttgtct cccatagtca ccattaggat cgtcatatct
    11401 cattcttgac caggcaagta cttcttctgg agataacgta agggagcctt ttgggaaatc
    11461 atagtgatca aagctgaaag caaaatcatt gaccacttca atatcgccaa cgatacttag
    11521 aatactctcc agccctttca tattcattgc catgtagtag ggaatagtag tatcaagcat
    11581 actctctgtc gtttgaatag cagtttgttc tcctccgtaa gcataagcgg cattcaattt
    11641 gtcaaatctg ttttgtcctg cgatttgaac ataggtatct cgctctagac taatcaattt
    11701 tattgcttca tttttcagat caacatgagc agcaatcatt gaatccgatc ttcctcgttc
    11761 cgtccttcca aattctccag tatctacacc taatagcagt acattgaacc gatcgggtaa
    11821 ttgtgtgtct gtatcggctg aatatttcac atttatgttg gcggctgttt tttttgaaac
    11881 ctcagacagg ttttgcaatt tcatatattc gcttacacca taagcacctg ctgctataat
    11941 aaacatcaat agaaaaatag atatgatttt taacgtttta tacatttatc ttctcctcct
    12001 ttgctgttaa ggatagaagc aaaatctatc tgatttgtat ctaaatacca tactttagag
    12061 aaattaaaaa aaggttgtgc caaaattctt gtcacaacct cttgataaac ggtattctaa
    12121 aagtagcttt ttcgaaaaat aatttattta ctgaactggc cgatggtcat ttctgccata
    12181 atagttctct acagccgttg gatgccctaa taaatcgcca actgtcgtga tgcgata
//
LOCUS       DACCSK010000084        12237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCSK010000084 DACCSK010000000
VERSION     DACCSK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.085"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(147..488)
                     /locus_tag="IVP39_002017"
     CDS             complement(147..488)
                     /locus_tag="IVP39_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7587081.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(556..2727)
                     /locus_tag="IVP39_002018"
     CDS             complement(556..2727)
                     /locus_tag="IVP39_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7587082.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2938..3795
                     /locus_tag="IVP39_002019"
     CDS             2938..3795
                     /locus_tag="IVP39_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7587083.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3866..4723)
                     /locus_tag="IVP39_002020"
     CDS             complement(3866..4723)
                     /locus_tag="IVP39_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7587084.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4708..7410)
                     /locus_tag="IVP39_002021"
     CDS             complement(4708..7410)
                     /locus_tag="IVP39_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7587085.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7423..8712)
                     /locus_tag="IVP39_002022"
     CDS             complement(7423..8712)
                     /locus_tag="IVP39_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7587086.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8862..9908
                     /locus_tag="IVP39_002023"
     CDS             8862..9908
                     /locus_tag="IVP39_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7587087.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            9910..12063
                     /locus_tag="IVP39_002024"
     CDS             9910..12063
                     /locus_tag="IVP39_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7587088.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         3372 a         2581 c         2240 g         4044 t
ORIGIN      
        1 tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag
       61 acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac
      121 accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg
      181 atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca
      241 ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg
      301 tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag
      361 tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac
      421 gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg
      481 tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac atggggttat
      541 tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc
      601 aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg
      661 ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt
      721 gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact
      781 aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc
      841 gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat
      901 ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt
      961 ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac ctgcaggatt
     1021 cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc
     1081 tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc
     1141 agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa
     1201 tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc
     1261 taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata
     1321 tcctttttgt aaaccgatct catgcaaaag ccatacagca ggcaaattca aactttgtgc
     1381 aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta
     1441 ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg
     1501 tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa
     1561 gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt
     1621 attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg
     1681 gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc
     1741 tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac
     1801 tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat ctgaattatc
     1861 gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt
     1921 tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt
     1981 ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc
     2041 agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg
     2101 gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc
     2161 tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa
     2221 cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc
     2281 gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat
     2341 atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat
     2401 atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc
     2461 ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc
     2521 gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa
     2581 accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg
     2641 atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat
     2701 cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt
     2761 cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt
     2821 ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt
     2881 aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag tacacttgtg
     2941 aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa
     3001 aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg
     3061 atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg
     3121 ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc
     3181 cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact
     3241 catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac
     3301 actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt
     3361 ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg
     3421 atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc
     3481 aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt
     3541 cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat
     3601 tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca taagggacat
     3661 ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca
     3721 ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct
     3781 ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc
     3841 tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac
     3901 taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag
     3961 gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat
     4021 gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat
     4081 agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg
     4141 gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt
     4201 ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca
     4261 cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga
     4321 cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg
     4381 ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa
     4441 tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag
     4501 ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc
     4561 caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt
     4621 cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg
     4681 cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa
     4741 tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc
     4801 ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa
     4861 atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa
     4921 agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt
     4981 tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa
     5041 ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc
     5101 acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact
     5161 gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa
     5221 gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg
     5281 cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa
     5341 aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg
     5401 atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct
     5461 gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg
     5521 ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg
     5581 gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt
     5641 tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa
     5701 gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg
     5761 tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg
     5821 gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc
     5881 ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa
     5941 agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata
     6001 accaaattgg acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc
     6061 tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc
     6121 ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat
     6181 tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat
     6241 tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt
     6301 ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc
     6361 gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt
     6421 tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg
     6481 gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata
     6541 ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac
     6601 atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg
     6661 aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc
     6721 aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt
     6781 ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata
     6841 ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc
     6901 tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt
     6961 tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg
     7021 tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg
     7081 gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc
     7141 atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg cttgttggat
     7201 tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc
     7261 caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa
     7321 ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct
     7381 atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat
     7441 atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct
     7501 cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc
     7561 agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc
     7621 ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc
     7681 ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt
     7741 ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt
     7801 gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata
     7861 aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc
     7921 tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat
     7981 ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata
     8041 acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt
     8101 tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt
     8161 ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc
     8221 ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc
     8281 caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt
     8341 catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc
     8401 atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc
     8461 taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct
     8521 taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt
     8581 gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg
     8641 gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc
     8701 tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca
     8761 tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta
     8821 tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc
     8881 agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc
     8941 aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac
     9001 gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt
     9061 tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc
     9121 tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc
     9181 ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata
     9241 aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag
     9301 aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag
     9361 ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg
     9421 cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta
     9481 cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca
     9541 aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc
     9601 tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta
     9661 tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta
     9721 tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac
     9781 aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa
     9841 aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg
     9901 agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat
     9961 ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca
    10021 ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa
    10081 ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt
    10141 acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc
    10201 ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc
    10261 tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat
    10321 gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac
    10381 acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat
    10441 tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat
    10501 agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca
    10561 tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa
    10621 gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc
    10681 acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta
    10741 tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact
    10801 agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa
    10861 acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt
    10921 tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt
    10981 ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc
    11041 cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc
    11101 aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct
    11161 aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt
    11221 agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca
    11281 ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat
    11341 ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt
    11401 gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt
    11461 ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac
    11521 ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga
    11581 caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga
    11641 caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct
    11701 ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat
    11761 agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta
    11821 attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct
    11881 aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa
    11941 ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc
    12001 atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat
    12061 taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat
    12121 agatcctcat atttttcgtt tggttggtct tattaccgca gatgcaagct tttgaacacc
    12181 gtttattgag aggttgtgaa atcagcgttc tgacctgagt attagagaga caaatgg
//
LOCUS       DACCSK010000085        11612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCSK010000085 DACCSK010000000
VERSION     DACCSK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.087"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            104..967
                     /locus_tag="IVP39_002025"
     CDS             104..967
                     /locus_tag="IVP39_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7587089.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1096..2718)
                     /locus_tag="IVP39_002026"
     CDS             complement(1096..2718)
                     /locus_tag="IVP39_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7587090.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2967..3488
                     /locus_tag="IVP39_002027"
     CDS             2967..3488
                     /locus_tag="IVP39_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587091.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3674..5404)
                     /gene="spxB"
                     /locus_tag="IVP39_002028"
     CDS             complement(3674..5404)
                     /gene="spxB"
                     /locus_tag="IVP39_002028"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7587092.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5569..6387
                     /locus_tag="IVP39_002029"
     CDS             5569..6387
                     /locus_tag="IVP39_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7587093.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6490..6687)
                     /locus_tag="IVP39_002030"
     CDS             complement(6490..6687)
                     /locus_tag="IVP39_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587094.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6856..7434
                     /locus_tag="IVP39_002031"
     CDS             6856..7434
                     /locus_tag="IVP39_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7587095.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8479..8982)
                     /locus_tag="IVP39_002032"
     CDS             complement(8479..8982)
                     /locus_tag="IVP39_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7587096.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9341..10366
                     /locus_tag="IVP39_002033"
     CDS             9341..10366
                     /locus_tag="IVP39_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7587097.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10713..11591
                     /locus_tag="IVP39_002034"
     CDS             10713..11591
                     /locus_tag="IVP39_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587098.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3598 a         1975 c         2258 g         3781 t
ORIGIN      
        1 tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag atttgctata
       61 atagacgata agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac
      121 agattcttgt tgtgatctgc cataccatgt attagcaaaa gaaaaagttg attttttatc
      181 aatgaatctc caattgaatg atcaagagtt agtagatgac ctcggaaaga cattcaatta
      241 tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt
      301 tggccgatat ttagaatttt tccgttctta tgcggaaaaa cagattcctg ttttatatgt
      361 ggctttttcc agtggattaa gcggctctta cagtagtgcg cttcaagcgg ttgaattgct
      421 gaaggaagaa tatgaagacc ctgagatcta tgtttttgat acaaaagctg caagtattgg
      481 ggaagggctt ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga
      541 aaccctagag tggctggaaa gtaattattt gaaagtccat tcatgggtaa cagtagacga
      601 tctgaaatat ctagaacgag gcggtaggat ttccaaagca agtgctactc ttggcagtct
      661 attgagtgta aagcctatta ttacggtgga taaagaagga aggctgacga atgttgacaa
      721 agttcgagga agaaacaagt cgatccagac aatcgtcaat gagacggtaa aaacaatcgt
      781 tgcaccactt gatcaaatcg tttatgtggc ttacgctggg gatctagaga gcgcggagaa
      841 ggcaaaacgg cttttggaag agcagataaa gatgaaagat gtgaagcttt atcccttagg
      901 accaacgatc gccagtcaca caggctatgg ctgtatcgcg attttttcga tgggaatcag
      961 tcgataaaag cagtaactca ttctgtaaaa aagcccagaa attgattgta acaaaaacag
     1021 cgactcttaa aagatagatg agcatcctat ctcttagagg tcgctgtttt tttatttttt
     1081 aagctaaata ggccattatg cccataactc gttcaattgt ttttgaatat ctgcattttc
     1141 caaaaattca tcatatgtcg tatcagctcg atcgatcaca cctttatccg aaacggcaat
     1201 gatgcggttt gccaatgttt ggataaattg atggtcatgt gacgcaaata agattgagcc
     1261 agtaaatgcc atcaagccgt cattcaatgc tgtgattgat tctaagtcta aatggttcgt
     1321 tggatcatct aatacaagta cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg
     1381 gactttctct ccaccagata aaacattcac tggtttcaat acttcttcac cagaaaacaa
     1441 cattcttcct aagaagcttc ttaagaaggt gttatcatct tcttctttac ttgcatattg
     1501 acgcaaccag tctaaaatcg ttagatcagt atcgaattct ttcgttgtat cctttggtaa
     1561 ataagaatta cttgttgtca caccccaacg tactgttcca gtatctggcg taatttcacc
     1621 catgataact ttgaatagcg ttgttgttgt tatatcattt tctgcgacga aagcgacctt
     1681 gtcttctttg ctcaaattaa aagatatatc atctaaaatt ttctttccat caatcgttac
     1741 acttacattt tctacctgca ataagtcgtt tccaatctca cgttcaggtt tgaatccaac
     1801 gaatggataa cgtctagaag aaggttggat atcgtctaaa gtaatttttt caagcatttt
     1861 tttacgtgaa gttgcttgtt ttgattttga tgcattcgca ctaaagcggg caataaattc
     1921 ttggagttct ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa gtttagccgc
     1981 tagttgacta gattccaacc aaaaatcata atttcctaca taaagtttga ttttaccaaa
     2041 atctagatcc gccatatgtg tacatacttt gttcaaaaag tgacggtcat gggatacaac
     2101 gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg attggatatc
     2161 cagaccgttt gttggttcgt ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc
     2221 taataacact tttacctttt gtccaccagt taattcactc atttgctgat ggtgcaattc
     2281 ttcagggatg ttcagacctt gcagtaaaac agctgcttct ggttcagctt cccatccatt
     2341 aagctcagca aattctcctt ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc
     2401 ctctttcatg taaatcgcat ctttttcttt cagcacttca tacagacgct tgtgtcccat
     2461 gatgactgtt tcaagtacag tatagtcttc atagtcgtaa tggttctgtt tcaatacggc
     2521 catccgttca tctggtccca tagtaacgga acctgtggaa ggctgcagat cccctgataa
     2581 cactttaagg aacgttgatt tacctgcacc atttgcacca atcaagccat agcagtttcc
     2641 gggggtaaat ttaatattca catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac
     2701 atcatttaca gtaatcaatt tttttcctca cttttatcat tattagtttt gacaagtctt
     2761 atagaattat ataggggaaa ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca
     2821 cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta ctataggatt
     2881 tattagtttg caacagcata atttttgacg ttatttataa gagggagtac tataaaactg
     2941 taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat
     3001 tgattttggg aattttattt gtacttgtct cattgttatc atttcaagac cctgtcggaa
     3061 atttagttgc aattgtagta gtctttgcga tttttgcctt tattaaaggg atatttgaac
     3121 tgtttgtacg gaacagatta aaagagctaa caggatataa aggaaagatg cccttaataa
     3181 tcgggatcat cgatattcta gttggcgtct ttttcttatt caatattggt gcaggagtcg
     3241 cagcattgcc ttttgttttt gcagtatggt ttattgctga ttcagtttta gcgttattta
     3301 ctgctgactt ggcaagagga gtaagtgaag gatattattg gtttacgatc atcgtcaata
     3361 ttttaggaat tttattagga attttcttat tgtttaatcc aatttcttct gcgttgacat
     3421 taagcttttt agttggattc tattttatgt tatttggtat cacacatatc gtctatgcat
     3481 ttagataagt aaaatcaaac aaataaaaaa gccggcttcc gattacagtt ttactgttgt
     3541 aattggaagc cggctttttt atcatggaca aatatgcacg aggtgatttt taggtgtcga
     3601 gacaaattta tgcaaaaatg tgagaagcta tatacacaaa ttttatctta atactcgaag
     3661 catagcgcag ttgtcacaac ctcttttcat ataattcatt caatactggc aaatcatgga
     3721 cttcatattt ttctttgaat gcttggattt cttcttcaga atattttctt ggatctaaat
     3781 gcagatcttc tactggcaat gggcgcttgt tattgatctt cacatcgatt actacaggac
     3841 gatctgattt tgcagctgca tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa
     3901 agctatttgc cccgagcgct tctccaactt taccgaaatc tgcatcttct aaaaatacac
     3961 caaatttctt ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg
     4021 aaaagacaac attaataatt ggaagctgat atttgacttg tgtaatgata tcttgcatga
     4081 ccatagcaaa cccgccatca ccacttaagg taaatacttg tttttctggg tagcttagtt
     4141 gtgcggcaat tcctcctggc acaccattac ccatagttgc gaaccatcca gatgtcgtat
     4201 gtttttgttt accgttcata ttcaataaac gaatagaatg gatagtcgtg ttaccgacat
     4261 ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat ttctttatag acagcctctg
     4321 gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa ccaatttacc catttttctt
     4381 tattttcttg atttgctagg taccagctat cagcagggcg agcatcccct aactcgacaa
     4441 gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc
     4501 caaattttga cgcatcgata tcgatttgga taaattcagc agttggatta aaaaatgcac
     4561 gtccaaatgg gaagtcgcta ccgacaaata agatcagatc ggcttctgcc aatgcttcat
     4621 ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa
     4681 taattccttt tgctagaacg gaagcgacaa caggaactga aaaatgttct gagaattctt
     4741 tgatttgcgg aaatccgttt cgcgttcctt gaccaacata aagaactggt ttttttgctt
     4801 tttcaagata aggcaaagca gctagcaaat ctttttcttc tggtaatatc acacttgttt
     4861 tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag
     4921 ggatagtcac gatagccacg cctttatgct cataggcggc tttaatggct tcatctacta
     4981 catgcggaag actttgtggt gtcatgactg tacgattgta tacgctaaca tctgcgaaaa
     5041 taggattttc atttagttcc tggaaagagt tatagttcat ggatgtagaa gcaacttgtc
     5101 ctaagaacgc tactagaggc acatggtcca tctgtgcatc atacaaacca ttgatcaaat
     5161 gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac tttccccgtt aattttgcat
     5221 ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag ttgatttcgg
     5281 ttttttcttg atatagcgca tccataattg aattgaaaga tcctccggga atgccgtaaa
     5341 tatggtcgat accccagctt tcaagaactt ttaccattgc gattcctgca ttgatttttg
     5401 acatggtgtt tcactccttt agcaatgatg atacataaat tttttgtatc tagagtatag
     5461 aactttttat cgaaaatccc aactaattcg tcttttcaca aaaatgtagt catgtttttg
     5521 ggaatatgct agagtagtac atagatagat tcaagagagg ggaatggcat ggttgaatgg
     5581 ctattgagac ttgaaccttg gcagcaagca cttgctggga cagcttttac ttattttatg
     5641 acagctcttg gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac
     5701 ttgatgttag gatttgcttc tggtgtcatg attgctgcaa gtttttggtc tttgttagat
     5761 ccggctatta caaaagcaga agaaaatgga gatattgctt ggctagttgt aagtattggg
     5821 tttggtttag gcggtgtgtt tttatatatg gcagataaaa cactgcccca tatgcatttt
     5881 ggaccacaac atgaagcaga aggattgcct acccatttaa aacggacgat cttgcttgtt
     5941 ttttctatta cattgcataa cattcctgaa ggtttagccg tgggggtagc attcggtgca
     6001 gcagcaactg cggataatcc gacagccgct atattagccg ctgtttcggt ggcgctaggt
     6061 attggtatcc aaaacttccc agaaggagca gctgtatcaa tcccgttgcg acaagaaggt
     6121 ctgagtcgta aaaaggcctt tgtttacgga caagcttctg gaatcgttga accaattgct
     6181 ggagtgattg gagcgctact agtaacgaaa gtggagctgc tgcttcctta tgcacttgct
     6241 tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat tgatccctga agcacagcag
     6301 acattgtcca gcaaacgtca ttttgcagtt tttggcgtca tgagtggatt tatcattatg
     6361 atgattttgg atgtggcatt aggataaata aaaaaaatat atatgatata tcaaataggg
     6421 ccgcgaactt atatcgcgac cctatttttt tagtaagtta tatcataatg aagtgaatgg
     6481 tatcatacat taataatagt tttttctatg tgtatttcct ttgatccagc gaacaagcca
     6541 gatgataagt gcgattggga ttagaacttt gaccgcaaac cataaaagcc cgcttaggac
     6601 actgacaaaa agactgatga cgaagctagc tgcaattagc actaaaagaa ccatgaagat
     6661 atttaagaac atattttttt gacgcataca aaaccctcct cttgttcatt ctttactata
     6721 gaaaaagtat aacagggatt gtttaaaaac acctccaact gaagactgat ttttacttca
     6781 tctacttaaa tgatcaaaca attgcttttt ctctggttta tcatctaaaa tagagcggaa
     6841 aaggaggaga atatcatgcc taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg
     6901 attatcatga ctattttagt tttagaactt catgaaccta acggagacac gtgggaggcg
     6961 ctcgctgcat taggttaccg attttttatc tatattgtca gttttatcat tttagcgatt
     7021 tattggaaca atcatcatca tttatttcaa gttgtacata agataaatgg acgggtctta
     7081 tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc
     7141 agtgagtaca tcaatagcct ggctccagaa atgacttatg gttttgttac cttgaccgct
     7201 aatattgcgt attatatctt ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa
     7261 gcattgaagg cgatgttgaa taattacaag aaatcgtatc tatcgattgg tttgaatgtg
     7321 ttaggaattg tcttagggta tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg
     7381 attctagctg cttggatcat cccaagtcgt accatcgaat cacaatatcg gtaaaatgat
     7441 ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa taaacgatca aatatccgag
     7501 tgatactagt attcaaagag ggatgcagat ggatcaaaaa agaattcatg taggactgat
     7561 tactagtttt gtcacgatgc ttatttattt ggatcaaggt catttgactt ggacagtagt
     7621 tggtgtcgta ggatttttga cattattcac agcaataaaa aaataaacat ctttatgtaa
     7681 tctgatgttc tatctattta agaaaagcat gtttacagca gtatgattct gttgtaaacg
     7741 tgtttttatt tgtggaaaat ttaattttta gaaaagtgta tcgaaaaata gatagttagt
     7801 ttttccacag tggataactt cgtaagagat aaaaataaaa aattagtcaa agaagggcgt
     7861 atagatttct tttgatagga gatatacaac agaaatgaat aaatattaac gtgttttcaa
     7921 acagtataaa acggtgtggt ttttggaata aaaaaataac atatctcatt ttacgctttt
     7981 gttattttat acttaaaaaa taacgacaca gaaaataccg tgtctcagat ttctctacta
     8041 cttctaaaaa ctactgtttt tttcctttga aatgccttca aaaagtagga tcaggcagaa
     8101 tatactggca gaatcaagga gaagggagcc taaataagga gaaaaaccat agaaaatata
     8161 aagtaattac ttataaaaca agctttttat agaaaaataa tatacctgag tagggtataa
     8221 attaagacgg aattcctttc tagattgaga tattgactag gaagaatgtt aagattttaa
     8281 gcctttaagc taaaaaaaaa ccctttttgt taaataaggg gaatatagcc tgtcagtttc
     8341 aaactgcaag tcgtcctgct gcaataatat agtagattag agatgaaaat agattttttt
     8401 aaaccaatga ctgaataaaa agaccgtcat agatacacat tggaatgtgt ctattacggt
     8461 ctttttttac gaacgatttt attgaagacg gacagttcgg gtcaaaaata aaaaagctac
     8521 gatcagaaat aagaataaaa tgtatcccaa aataatggct agacggtcta acttatttgt
     8581 cgatagatgt ttgtttagaa gttgataggc ccataaacct atcagtcctc ctgttgtatt
     8641 agttatcaaa tcagtgatgt ccgctacgcc aataccgaat aggaattgaa tcaattctgc
     8701 caagaagctt acaaggaaaa taatccctaa ttttttagaa aaagaaagtc tcttgaaatt
     8761 gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa
     8821 taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg agattgacat tgctgtaaga
     8881 ataagtgaga aattgtggaa gattaaatga tagcttgaat aaaatcaacc ataatagaaa
     8941 gaataaataa atccctaata aaaattttga tacataccgc atagacatct cctccattta
     9001 aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa
     9061 cgtagttatc gtacgaaaat tagaagcttt tttttgttgg gaatccggtt tctttaacaa
     9121 agctaagata gcgagttgct tggaaaagtt gctatctttt ttctgtcaaa acgactcgga
     9181 atgttcgttt tttgttctaa tttctccaag ttgtttactt tttgatacta agtttatgaa
     9241 aaatcaagaa atagttgtta tactaggtaa tgctgacaaa cgggattggc atttcatctt
     9301 ggttcagctt tttttccaat tactaggaag gagggataga ttgttcaaga cttattttcg
     9361 tttcaataaa atacaagata atccttttcg tatcatgaat ctgatgctac ttatctttat
     9421 tgtattatgg ttaggttaca taaatcatga ttttttgatt tcaagttttt catcattagg
     9481 gatctttact tttttctatt atcaaaacat accgatgaaa tatttgctga aacgtatgtt
     9541 tctgatcggt tgcggactgt tggttgcttt cgttttgggg atcttatcga cttatgtttt
     9601 atggcttgaa ccatttgccg tagctttggt tgctttttcc agcagattcg tgttgcgatt
     9661 attccatatt tctaaaccag gtggcttgtt ttttgcgatg ctttctgcca tgggaacgag
     9721 catgcagctg ccaatcgctc aattaccgat cgtctcactt tattttttta tgggtgtcgt
     9781 ttttgccctg attgcggcag tgattacaaa attactggat tcacggcctg agcagacaat
     9841 tgaaaaagca actttaaaag aaagatttca cgaagagcct ttagtgatca ttgattctgt
     9901 tttctacagc gctgccttat ttctttccgt ttatgtcagt cacggcttga atctgcataa
     9961 tccttattgg ttgaccttgt catgtgcatc gattttgcta gcagaaaact tggatgcgat
    10021 gaagcatcga caagtccaat atctgattgg atcgatgggt gggttatgtg tgtcggcatt
    10081 cttgtcattt gtgccgttta cgcagttgca gaccattttt ctgatcactt ttctatatgg
    10141 aatcgcacaa tttctagttg ctagaaatta tgcagttgct aatatttttc ttaatccgat
    10201 ggcgttgatg ctatctacac ttattcgtgg agcatacttg atttctttga tcgaatatcg
    10261 ttttttaggg atcgtcatag gaagtttcat cggtttaggt gttgcgtggg taatgacggt
    10321 tggtttgcaa cattatttaa cggttgttcg tcaaaaatta gaatagaagg acaaatatat
    10381 gtgccaaaag aggttatgac aagatttttg tcacaacctc taattcaagg tgttcaaaag
    10441 ccagattctc agcattaagt caaattcaca caaaaatgtg gaaaggaact ttcaaaaaaa
    10501 ttttatattt attctatcaa aaaattttat atttattcta tcaaaaaatt ttgtttatga
    10561 tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc ttacagatat
    10621 tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta tgtttatacc
    10681 atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg aaaaggggaa
    10741 atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta actcttccca
    10801 aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag gtgtttgagc
    10861 aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag aaaatagaca
    10921 aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca gcttctcaaa
    10981 gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg gcaacaatga
    11041 atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat cgctttgacc
    11101 gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt gaattggagt
    11161 acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga aagcagtgcc
    11221 tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga gatagttacg
    11281 aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa gtgatgtaca
    11341 cagatacttg gttactatca cccaatctta ctaaatggtt gaaaaaagag tcaaaaatct
    11401 gtctttttgc agccgattat cgattattat cagttgatga acaggacgat agtggagtcc
    11461 cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt acgagtttac
    11521 agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc ttaggtattc
    11581 ttacttcata agtttagtac ttcgaaatag tt
//
LOCUS       DACCSK010000086        11601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCSK010000086 DACCSK010000000
VERSION     DACCSK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.088"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(53..295)
                     /gene="acpP"
                     /locus_tag="IVP39_002035"
     CDS             complement(53..295)
                     /gene="acpP"
                     /locus_tag="IVP39_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7587099.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(417..1418)
                     /gene="plsX"
                     /locus_tag="IVP39_002036"
     CDS             complement(417..1418)
                     /gene="plsX"
                     /locus_tag="IVP39_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7587100.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1467..3503)
                     /gene="recG"
                     /locus_tag="IVP39_002037"
     CDS             complement(1467..3503)
                     /gene="recG"
                     /locus_tag="IVP39_002037"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7587101.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3620..5302)
                     /locus_tag="IVP39_002038"
     CDS             complement(3620..5302)
                     /locus_tag="IVP39_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7587102.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5354..5716)
                     /locus_tag="IVP39_002039"
     CDS             complement(5354..5716)
                     /locus_tag="IVP39_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7587103.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5986..6174
                     /locus_tag="IVP39_002040"
     CDS             5986..6174
                     /locus_tag="IVP39_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7587104.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6412..9081
                     /locus_tag="IVP39_002041"
     CDS             6412..9081
                     /locus_tag="IVP39_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7587105.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(9172..9792)
                     /locus_tag="IVP39_002042"
     CDS             complement(9172..9792)
                     /locus_tag="IVP39_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7587106.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            9905..10444
                     /locus_tag="IVP39_002043"
     CDS             9905..10444
                     /locus_tag="IVP39_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587107.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            10441..10779
                     /locus_tag="IVP39_002044"
     CDS             10441..10779
                     /locus_tag="IVP39_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP7587108.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(11160..11588)
                     /locus_tag="IVP39_002045"
     CDS             complement(11160..11588)
                     /locus_tag="IVP39_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7587109.1"
                     /translation="MDFFIYTKYFTLSINIIEELSRNSRITMKELGEKVHLTGPAVSA
                     RVTKLEESGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNY
                     KISGGGCYLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         3323 a         2327 c         2049 g         3902 t
ORIGIN      
        1 aaggataaaa agctgtatgc agctttttat ccttctttgc ttttatttga aattatttaa
       61 gatggctgct gatataatct actgcgccac cgactgtttc aatttgttca gcgtcttcat
      121 ctgaaatttc cgtaccgaat tcatcttcaa gctctaaaac gaattccata atactaattg
      181 aatcagcttt taaatcatct ttgatgttca attcatccgt cactttatcg gtatctatct
      241 caaaatgatt tgaaataatt ttcgctactt tgttaaatac ttcttcgcga gtcaactttt
      301 tcacctccaa gaatcataca cttgtcaatt acctacacca tggctaattt tactggcatt
      361 tttcaggaat gtccaccttt ttttcaatgg aacggattgc ttcagatatc caaatactat
      421 tcttcgcttt tttcaaattg ttcgactaat tgtccaacta catctgtctc aagcattgtg
      481 tggatttggc ggatcgtata gcgaacagca tcggggcctg ttgctccatg agttttaatg
      541 actggtgctt tcaagccaaa caaaacggca ccgccatgtt tggaataatc catttctgct
      601 ttcaaagaac gtaagccgtc ttttaaaagc atagcgccca tttttccttt gatcccttca
      661 tttagaatag cagattttaa tagattcatc atattcattg ctgttccttc aatagatttc
      721 aaaacagcgt tgcctgtaaa tccatctgtg accactacat cagccacacc attgagtagc
      781 tcacgagctt ctacgtttcc aataaaattc aagcttgttt cattctgaag gagttcatag
      841 gcttttttcg tcacttcact accttttgtt gcttctgttc cgttgtttaa gagtgcgaca
      901 cgaggacgtc cgatacctcg gactttacgt gcataaaaag aacctaaaat accgtattgc
      961 aacaaatgtt ctggtttgtt ttctgcgtta gcgccaagat ccagcatatc gaaaccggca
     1021 ccttctttcc ctactactgg caaggtagac atcaagccag ggcgttcgat atttttgata
     1081 cgtcctacga tgaacaaccc tgctgctagt agcgcgcctg tattccctgc tgaaaataca
     1141 gcatctgctt caccgttttt cactgcttgt gcagcaagga ccattgatgc agtctttttt
     1201 cgtcgaattg cttttactgg ctcatcatca ctattgattt tttcgtctgt atggataatc
     1261 gtgatgtttt tttcatttgt taagtatttc ttgatttctg cttcttttcc gtaaagtaag
     1321 aattctactt ctggaaaatc ttgctgcgct aaagccacgc cttctacaat tgcttgaggt
     1381 gcgtgatctc cgcccattgc atctacggca attttcattg tttccactcc tttttccgtc
     1441 atcagtataa agtatagcac agtctgctaa tcgaaataac gattatcctg attttctatt
     1501 tggaggcttt gcgctaaagg ctgatactca ggaagcatct gccaatcctt tatctgccaa
     1561 agggctgcag cctcttctct ggctacctcc aaaacatttc catccgtcac caagtcagca
     1621 atcgcaaatt gaggcagacc agattgacgg aagccaaata cttcaccagg accacgtaat
     1681 tccagatctt tttcgcttag tacaaaacca ttggtggtct ctgtcatgat tttcatccgt
     1741 tccacaccta gttcattttt aggattagca actaagatac agtaggaagc atcacttccg
     1801 cgtccaacac gtccacgaag ttgatgcagc tgtgccaatc caaatcgatc cgcatccatg
     1861 atcagcatga cggtcgcgtt cggcacgttt acccctactt caattacagt agtcgaaact
     1921 aatatctgca tctggttgtc tttgaaggct tccatgatta cttctttttc ctgattcttc
     1981 attttaccat gtaacaagcc aacttcatat tttggtgcaa atagttcacg aagtttttca
     2041 tagatttcca ctgcattttt tacatctaat gcttctgatt cttcaatcag cggacagatg
     2101 acatacattt gatgtcctct tgccagttct tttttcgtcc aatccagtac actgtccaat
     2161 tgaggtgttc ttacccagcg tgtttcgatc gggatacgtc cagctggaag ttcatcaatg
     2221 atagatacat ccatctcacc atatgctgta atagctaatg ttctaggaat cggagtcgct
     2281 gtcataaata agacatctgg tcgccagcct ttttcacgaa gtactttccg ctggttcacg
     2341 ccaaaacggt gctgctcatc cgtaataact agacctaaat tctgaaattc gactccatct
     2401 tggatcaatg catgtgtgcc gatcacgatg ttaatttccc cttgctctag tttttcaagg
     2461 agctcccgac gttcttttgc tttcgttgac cccgtaagca atgcgatggt tacttcattg
     2521 ggatcaaaca gctgttgcaa gctttccata tgttgttgtg ccaagatttc tgtaggcacc
     2581 atcaacgcac cttggaaacc tgctgtaacg gtagcgtata acgcaatcgc ggcaaccact
     2641 gtttttccgc tacctacatc tccctgcaaa agtcgctgca tatgctgcgg actgcgaaga
     2701 tcccgacaga tttcattcgt tacacgtttt tgtgcatttg tcagttcaaa aggtagcttt
     2761 tgagtaaatt ctttcaattt ttggacatca taaaggatct ccaaaccatt cgtttctgct
     2821 ttttctgctt ttttcaatcc ttgaagacgc atttgaaata aaaagaactc ttcaaaaacg
     2881 acacgtcgtt tggcttgatg gttttcttcg tggtcttttg gaaaatgcat acttcttaca
     2941 gcagaagctc ggtcaagcag tcgatatttc tccacaaggt tgtttggcag attttcttca
     3001 atcaatgtgc cgtattcttg aaaccctttt ttgattagat caactaatgt cgtttgacga
     3061 attgatttgt tgacgtgata tatcggcgaa aagtcatcca ttgtttgaga acctaaaatc
     3121 ttcatcccgt tcaatgcttt acgctttgca tcccatttac cgtatacggc gatttcctcc
     3181 gataaaatga ctttttcttt tagataaggt tgattaaaaa aagaaacgtt gaacacatca
     3241 tgttcttgca tcatacgaaa ttgcaatcgg ctttttttat aaccaaatcg attcatcaca
     3301 ggaggcgaga ctacgattcc tttcaaagta accttttctt gatcttggat ctcgtttaga
     3361 tttctttcct gaatatcttc gtagcggaaa ggataataag tcagtaagtc ttcaatcgtt
     3421 tggatgccca agctggcaag actagctgct cttttttctc cgactcctgc cagcatagtg
     3481 attggatcat ctaaagaacg cataaatttt tcctccctcc tttaacaaaa aaaggacttg
     3541 gtcagcgatc atacctgatc actaaccgaa ggccttttat gaatgaatac tgtgttagaa
     3601 agatattctg acacagaaat tattcagcag aaaatagata aggatagaca ggctgatctc
     3661 cttggtggat ctcgatttcc aaatcaggat gtttttctgt tagtttctca gcaaattcat
     3721 ttgcttgttc aactgttcct tcatcacctg ccaagatcgt tacgatctct gtgtcctcat
     3781 tgatcatttg ttctagtgta cttaacgctg ttgctgtgat atctgggtta gagatcagga
     3841 tttttccatc gatcatgccc aaaaagtctc cttctttgat ctctacaccg tcaattgagg
     3901 tatctctgat cgcgtgagtg acttgaccgc tcacaacgct agacaacatt tctgtcatcg
     3961 tttctttgtt ttcttctaaa gaagcttgat cattgaatga aagcatagct gtcaatcctt
     4021 gggaaatcgt tcttgaagga acaactgcta ctgggatatc tgctacttct gctgcttgat
     4081 cagctgccat gaagatgttc ttattgtttg gcaagacaat cactttatct gcgtttactt
     4141 cttgaatcgc tttaacaatg tcttccgtac ttggattcat tgtttgccca ccactgataa
     4201 cataattagc gcctaaactt tcaaacagct cttggacgcc tttaccagca gcgatcgcta
     4261 cgataccata cgggatacgt ggtgtttctt ttttcgcaaa ctcgttcact tcttcatcgt
     4321 gttccaaaat cgtctcatgc tgcaagcgca tgttgtcgac tttgattttg actaatgaac
     4381 cgaatttttg tccataattc aagacttctc ctgggttttc tgtatgcacg tggactttga
     4441 tgatctcgtc atcattgacc acaagtaaag aatctcctat accatctaaa tagttacgga
     4501 attcttcgta atcgaatgta ctatcgactg taggtccttc tccgattttt accatgattt
     4561 ctgtacagta accaaaatgg atatcttctg tcgccatttg accttggaca ctgcgatgat
     4621 gttctgcatt gaccatatca tccatttctg ctggagaagg ttcatatgtt tcatccgctt
     4681 ggaattctcc attcaatgca cttaaaaagc cctcataaac aaacagcagt ccttgccctc
     4741 cactgtctac gacaccgact tcttttagga ctggaagcaa gtctggagtt ttagctaaag
     4801 cacgctttgc cccttttaca acagctgtca ttacttcaac gacatcatca gtactttttg
     4861 ctttcttttc tccagctttc gctgcttctc tagctactgt caaaatcgtt ccttctactg
     4921 gtttcattac tgctttatac gcagtttcaa ctccatgagt gaaggctgca gcaagatcgg
     4981 aagcatttaa tgagacaaca tctggaattt gcttagaaaa tccacgaaac aattgagaca
     5041 aaataacgcc ggaatttccg cgcgcaccca tcagcaatcc ttttgaaagc attgctgcta
     5101 attctcctac tttttctgag gttgaatcta ctactgcctt agctccacta gtcatagata
     5161 gattcatatt tgttcccgta tcgccatctg gcacagggaa tacatttaat gaattgacgt
     5221 attctgcatt tacttgcaga cgattcgcac ctgcctggac catttcctgg aactggcttg
     5281 cacttatttc tgttacactc acctagataa tcctccttca gatcgactgc tgagttacca
     5341 gccagtctcc tgcctagtct ggcagtacac gaacaccttg cacaaagaca ttaactgaat
     5401 tggcagttac gcctaataat gcctctagat tgtatttcac tttttcttgg acgttgcggg
     5461 aaacttcaga gattttcgta ccatagctga taatcgtata aacatctaca gcaataccat
     5521 tatcttcttg gcggacaact actcctcttg agtaattttc tttacgcaaa atctcagtaa
     5581 tattatcttt aatttgattt ttgctggcca ttcctacaac accgaacaca tctgtcgcgg
     5641 cgccaccaac aactgttgca attacatcat tggtaatttc aatggttcca gctgacgttt
     5701 gaatttttac agccatgaat gagcctcctt aacgggtaaa aaactcccat ttttcttcta
     5761 tcatatttta tcatagcgca acttgaaata aaaggcagtt acccaaagta ctcaaaaacc
     5821 ttgcaaaatt tcaggtataa ccttgtttaa tttacaatta ttcttctcaa ttgtcaattg
     5881 tatctatgaa aatgtacttg caaatttcca ttctatatga tatgttagtc tggtatgagc
     5941 tgaattattc tattgctcca aatcaaggca aaggaggaaa ttaccatggc aaaagtttgt
     6001 tactttacag gtcgcaagac aaaaagtgga aacaaccgtt cacacgctat gaactcaaca
     6061 aaacgtactg ttaaacctaa cttacaaaaa gttcgcgtta tggtcgacgg aaaacctaaa
     6121 aaagtttggg tgtcaactcg tgctttgaaa tcaggaaaag ttgaacgcgt ttaattgaat
     6181 gatacttgga ccgttcagat agtgaacggt tttttattta ataaaaattt ttaagggtgg
     6241 gacttttgat tcctaccctt tttttgcgtt tatttttgtt atacgaacaa attatgaagt
     6301 tatttattct aaattttatt tttttgtgaa aactagactc tttttaagca gagaaaaaca
     6361 aggatatgat aaactgaata caacttcata gaaatttggt ggaaaataaa tatgaaaaaa
     6421 aatttaaagt cattctttag agaaaacgga cgctacgcag cagctagttt cctgattccg
     6481 tttttgattt tagcacttgt ttatctaagt atcgggatct atcctggaag cagcagaagt
     6541 attttggcaa gtgatgcctt ttcacagttt tctaattttc atgccagctt ccgaaatgtt
     6601 cttcttggaa agcaaagtat tttttataca tggaatgctt ctctaggatt gaattattta
     6661 tctttaattt cttactatct aggcgggatc ttcacgccac tggtgttctt ttttccaaac
     6721 cagatgatgc cagatgcact gtatgttctg actttgctta aggtcggctg tgccggattg
     6781 gctttttggt ttcttgcacg tacgttcaag attcctcgct gggggcatgt tgcacttagc
     6841 gtcagttacg cattgatctc atttatcact gcacattcgg agatcatcat gtggctggat
     6901 gcatttgttt atcttccatt agtgattttg gggattcatc gtgttatgga tcttaaacgg
     6961 ccgaaacttt tatttatcag ttatttactc ttgtttttat ccagttttta tatgggtttt
     7021 atgattggcg tattttcttt cttatatttt atcgcccgtc tattgacaaa ctggtcagaa
     7081 tataaaaaaa cgattgttcc ttatggtatc acttctttac tggcaggcgg cgcttcaatg
     7141 atcattgtat tgccagcagt gctggatctt cggtcaaatg gcgaggcact gagtcagatc
     7201 actacgttga aaacagaagc gacttcttat ctggatctaa tcatgaaaaa tatgattggt
     7261 gtttatgaca cgacaaaata cggatcgatt ccgttcgttt acgcaggtct gctaccatta
     7321 gtcctctgtc tgttttattt tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac
     7381 ggcagtcttt tcgccatact tatcgcaagt ttctatcttg ttccattgaa tttgttctgg
     7441 catggtatgc atgcaccgaa tatgttttta ttccgttatg cctatctgtt ctctttctta
     7501 gtgctcctgc ttgcaggaaa aggatgggaa aagatagaac aagacgatcg tgggctgttg
     7561 ttaggaatca ttttggtcgt agctgctctc tttgcggtgg catggggcat caaacctgaa
     7621 ggaagctaca cctatgtgac gatgacttcc tttgtattga cagtggtttt cttagggatt
     7681 tatgcttttg ctgtgggatt ttatcagcat aaaaaacttt cattcaatca tctgagtata
     7741 ttgttattat tgatcatgtc tgctgaagca ctggtcaata cgaacagtat gatccatggc
     7801 attcttgatg attggaatta tgcttcccgc agtttatata ctgatcctta tcctgacttg
     7861 aaagctcttg taaatgaaac aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg
     7921 gatccagtat ctgcaaacga ttcgatcaac tatggttaca gtggtatcag tttgttttct
     7981 tctataagaa atcgtaattc ttcctcttac ctggataaat taggtttccg ttcaagaggg
     8041 acaaatttga atatccgtta taacaataat actttgctaa tggatgggtt tacaggaatc
     8101 aaatataaca tttctaaaaa cgaaagcacg ttcagtaaat atggcttccg ccaagctggt
     8161 caaagtggcg actatacact gtatcagaat gtcaatgcat tgccactagc atttactgca
     8221 ccgttgtccg ttaatgatgt tgagcagcca gtgacagata atttgaccag tcagacaaac
     8281 ttgatcaata cattgtctgg tttaaatgaa cagtatttta cattctatac acctactttg
     8341 aagaatcagt tgaatacgac gatcacaaag acaacgactg gcgtcactta ctcagaaaca
     8401 gcagataacc agccaaaaga gctcacttgg gaagtccatg tgcctgcaaa tacacaagct
     8461 tacttaagtt tgttcccaac caattttgca caattagaaa gttctagtgc gacgatcact
     8521 gtcaatggta catcgcgaaa aacgcaaatc aacatttctg gtcaatacta tgatcttggc
     8581 tattatcctc aagagacaac agtgacattt actgcaagtt tttatggaac gaaagaagta
     8641 agtttcatgg agcctaaagt cgttggtcta gatgttgtag cctatcaagc cgcaatgaat
     8701 cagatcaaag aaaaaggtgt cgaaatggaa gccactggca gaaaagcttc tgggactgta
     8761 aataccgctg aagattccat gttggtgaca actattcctt atgacaaagg atggaaagct
     8821 aaaattgatg ggaaaccagt caaggtcagc aatttcaaag acgcattctt gatgataaaa
     8881 gttccagcag gtaaacatac tgtcactttc agttacttgc ctgaaggatt tacgataggt
     8941 gcaatccttt ttgtcctatg tattgcattg tttattcttt atgtctggta ccttgaccgc
     9001 aatcgcccct tttatggcac gattgatcca gcaatagaag aaaaaccgcg aaatacacac
     9061 agaagaagac gaagaaaata aagaaacgat ggaatctgtg tcactcttct atttaaatgg
     9121 caaaaatttc aatctttaaa tgacaaaaac cacctgattt ttggtggttt tttatatacc
     9181 tatgactaaa ctatctcttg ctgcttcgac aacttctaca gtgatcttat ccatttgatt
     9241 gattcttaaa atccgatcaa tctgcgcaaa taaacgatga ataagcctaa aatttccttt
     9301 tgtgatctta atgatagttg tgatagcctc ataatcagaa aaatcttcta gcttgatatc
     9361 aattcctaaa tctgcccatt tgtattccaa aatatgatgt gtttcgtcct tactaaggtt
     9421 atcaaattca tgtgcaaatc ctatgcgaga gtaaagctgc ggataacgag acaatctttt
     9481 ttcaatacca ggcataccga taagaaccat tgccagatca tttctatcgt aaatatctct
     9541 tagctgttct aaatgctgaa gctttaaacg atcaatttca tcgacaataa ttaagtcaat
     9601 tgcttcatag gttttaggca caggaggatc ttcttgattg tattttagta ccatatgcat
     9661 tgctttagtc agacctattc tactagttat tgtgttaata tccgaactga cacgagacat
     9721 cttttcagct ggtacagtat aaaaaacttt atttacactt aatatttcat ctgttgcatt
     9781 tatcaagaac aagaaaaagt ctgaatattc aaaaaaataa cggtgtacat ttttaaaagg
     9841 agatgtataa tcgagattga gaaagcgttt tcctcaaaat ttaatgaatt aggaagtgat
     9901 tactatgaaa aaattattta gcgcactttt tattctgtta tttggtttta ttggtattgg
     9961 gacttctgga aatatagtta aagctagcac gatggttgaa caaacaagtg aaatggcaaa
    10021 aacttttagt caattaacac cagaagaaca aagttacttc aaatccaaag gatttagtga
    10081 taaagatact ttttacactc aaaagataga gcctcaagat ccgaaaacac gtttttctgc
    10141 aaatgtattg cggttaaccg catctactaa aaaaattact aatacgcagg ggcggacaga
    10201 atatattata acaagtccca attcggcgat gtatcgtatt tctacacgct tgagtttagg
    10261 tagtgcaaaa tcatatactc aaacagtaaa tctagctaat tcttatggtt atggtggagg
    10321 aatgtatttt acatttactg ggaaaagaca atattacagt gtaaaattag ctatgcaagt
    10381 ttataataca tttggtgtag ggtcagtctc ttgcactgcg ggaggattga caataggaaa
    10441 atgataattt cttactatgt cttaaataga agtgatgatg aaggactctt cgtttttcaa
    10501 tcattacatg aggatacgat gctaaaagag ttttataatc ggattaagaa taaacagata
    10561 gaaacaaatt tatatttttt aagtgactct atcttgcctt ttattagcaa ccctaaaatt
    10621 cttaacttgt tgcagacaaa agatttgaaa agtttaccga ttattgtcat tgatgaggaa
    10681 attgtaaaat atggcgctta tctaagcgta gaagaattag aaaaattaac taacattgga
    10741 atgagtattc aggaggataa tggggtacat ggaacataga atgaatatgc acagttgaga
    10801 acggaatttg acatttattt agaccaacag ggtataccct gttggtcttt ttgtgcgttc
    10861 atgatatact tggtgtcatt tttatattta aatggcacaa atatttctaa ttatttgtca
    10921 tttaacgctg aaaatttttg ctatttatct aaaaaagtga caggatactc atttggcctt
    10981 catagaattt tctaaatatg aatcatcaat gaatttaatt aaaaaaataa gtagggcaaa
    11041 atataaatgt ttttgcctta cttgtttcca tcagtttagt tttaaattga taagttatat
    11101 agttatggtt gaatgtgtgt ctcgcatatt agtattttta ccatctgcta gttttattat
    11161 taatctataa caatagataa tttataattc gcatattcat taagctgttc caaaaagcta
    11221 ttcattgatt tttttgaagt aaatctacat tccaaaagat aacagccccc tccactaatc
    11281 ttataatttc taatcaagaa tttttcatga tttttaataa atgataaata gggtttatga
    11341 tttaaatttt gcgtaattat aattaaaaaa gcgtgaatcg aaaaccctag cttttccata
    11401 tttaatttta ctgtataatt ttcaataact ccactttcct ctagttttgt cactctcgcc
    11461 gagacagcgg gtccagtcaa atgtactttt tctccaagtt ctttcattgt gattcgacta
    11521 tttcttgata gttcctcaat gatatttata gagagtgtaa aatattttgt gtaaatgaaa
    11581 aaatccatac aaaaaaggaa g
//
LOCUS       DACCSK010000087        11282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCSK010000087 DACCSK010000000
VERSION     DACCSK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.089"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(173..430)
                     /locus_tag="IVP39_002046"
     CDS             complement(173..430)
                     /locus_tag="IVP39_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP7587110.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(555..1487)
                     /locus_tag="IVP39_002047"
     CDS             complement(555..1487)
                     /locus_tag="IVP39_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587111.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            complement(1893..2648)
                     /locus_tag="IVP39_002048"
     CDS             complement(1893..2648)
                     /locus_tag="IVP39_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7587112.1"
                     /translation="MDFEQNVRLNFDLLSDNEKDMVFFIRTHKAQVVHMSIVELGEQL
                     LSSKSSVLRLAKKLGFRGYSEMKYSLEQSIVQSVIPPTDLIALLKDEINRTFQLADQT
                     NFQPILEQLKAAKTVVVYATGFTQNNFSKDFVNDLILSGRPAMLISGETNFEMLSHTL
                     TKDDFVIVTSLSGETPSIKSTIKNLNMNRIPLCGVTELGKNFLSEHSDFLLYYETREL
                     PSNLIEGSRSMIGLNILLSILSRKYREFILFDE"
     gene            complement(2755..4425)
                     /locus_tag="IVP39_002049"
     CDS             complement(2755..4425)
                     /locus_tag="IVP39_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7587113.1"
                     /translation="MNEWWKSSVVYQVYPQSFKDSNNDGIGDLMGIVEKLPYLAKLGV
                     DVIWLTPIFDSPLIDNGYDIRDYEKISENYGTLEDFKRLLEQAHQLNIRVILDLVVNH
                     TSNQHAWFIESQKNKENPYSDYYIWKDPKEDGSAPTNWGSTFGGSAWEYVESRKQYYL
                     HCFAKEQPDLNWENPKVRQSIYKMMKKWFDLGIDGFRMDVISLISKHPDYPDAPNSMP
                     YTKSYYVGASNGPRVHEFLQEMNREVLSKYDSLTVGETPNTNSRQAQLYTFPERKELN
                     MVFHFDHMHLDYGKYGKFSDVRFKLSDLRATLSEWQEALSDGWNSLYWSNHDQPRAVT
                     RFGDENEYRIESSKMLGTLLHMMKGTPFIFQGEEIGMKNVPFSSIDEYQDIETNYFYK
                     KMKEEGESDEYIKRAIYLKSRDNARTPMPWDHSKGYGFSNSDPWISFSPDNQQINVED
                     ALKNPNSVFYHYQKLISLRKNYPIIQHGTYKLINENDSAVFGYIREYKNELLVVICSF
                     SKESVPYELPKSLQGRKGVLLLSNYEQSPSELEDKFILRPYEALVYHI"
     gene            complement(4429..4848)
                     /locus_tag="IVP39_002050"
     CDS             complement(4429..4848)
                     /locus_tag="IVP39_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HAP7587114.1"
                     /translation="MKKIIFASHHNLAVGLKDTVQYILPNVGEITAIAGYIDNSSIEE
                     KINEVMVRDNEDTDILIFTDMLGGSVNQAFIKYINQPNVYLITGMNLPVVLTILLNTL
                     NKRLTDNHIREAIQEAQDQLVYVNDYLKNIVVDEEDE"
     gene            complement(4861..5679)
                     /locus_tag="IVP39_002051"
     CDS             complement(4861..5679)
                     /locus_tag="IVP39_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7587115.1"
                     /translation="MILMNNEGNKLSKKDIRRAAFRYMFMACNIFNYENQQGPAVVYG
                     LEKALRKIYPNDEDYRASLNNHFKYFNTTTWMANLLLGASLAMEEKDGVSSLDAVQSF
                     KTGMMGPLAGVGDTLVWVLYPTIMGSIAAYMGLEGNPTGAIIWLCLNLFFLFFRFKLF
                     SIGYTSGLKLVTALGSKLSIFTESASIMGLTVVGALIPSVVKMNVAVVFQSGKVKLPI
                     QTEILDKIMPALLPAVLTIIVYKLIASKKMTVIQIIFSVIIFALILSFFGILKV"
     gene            complement(5663..6463)
                     /locus_tag="IVP39_002052"
     CDS             complement(5663..6463)
                     /locus_tag="IVP39_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7587116.1"
                     /translation="MEIIQGILIIILAVWVVLDQQGLVITTWFPIMIALFAGLIMGDM
                     TTAMVIGGTFQLMALGVANIGGSSVPNWGLAALVGIYVAIRTTNNIEEAKAVALAVGV
                     PVGMLGIQLDVLAKILNTYVTHAAQKAANKMDFKKMNQILWLGPVIFGLSTAIPTALC
                     VVFGDRIVRLILDFVPKWFTDGLSIAGSMLPVVGIALLLQVMPVKKYMTMLLIGFVLS
                     AYLNMPILGISIVGFALAYYFFTTEIKKAETASPVAANIVDEGDDFDE"
     gene            complement(6478..6954)
                     /locus_tag="IVP39_002053"
     CDS             complement(6478..6954)
                     /locus_tag="IVP39_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7587117.1"
                     /translation="MGLVLARIDQRLIHGIVVTQWAGATKAKRLMVVDDQVSKDDTQK
                     AAMRMSKPAGTGMSIIDTDTAIINFKLGKYDSHNVLMIVKEPSTLVKLAENGIDIPKV
                     NIGIMFDGEGKQTVKKMVSVDEQEIADLKKLEAMGIPVTFHFVPSDPEEPLDVRIK"
     gene            complement(7349..7828)
                     /gene="rlmH"
                     /locus_tag="IVP39_002054"
     CDS             complement(7349..7828)
                     /gene="rlmH"
                     /locus_tag="IVP39_002054"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7587118.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            8256..9542
                     /locus_tag="IVP39_002055"
     CDS             8256..9542
                     /locus_tag="IVP39_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7587119.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTTSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSGF
                     GGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKDG
                     TKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANS
                     VTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKI
                     ASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQE
                     QILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKV
                     GDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(9581..10186)
                     /locus_tag="IVP39_002056"
     CDS             complement(9581..10186)
                     /locus_tag="IVP39_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7587120.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLGADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWSGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(10391..10855)
                     /locus_tag="IVP39_002057"
     CDS             complement(10391..10855)
                     /locus_tag="IVP39_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7587121.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(10877..11197)
                     /locus_tag="IVP39_002058"
     CDS             complement(10877..11197)
                     /locus_tag="IVP39_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7587122.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
BASE COUNT         3444 a         2265 c         1767 g         3806 t
ORIGIN      
        1 cagttttaga aaaagcaaaa aaataaagaa acttattctt gataacaagc tacgtgacgt
       61 atatccctaa cacaaaaata gccatatcct ttggtttgga tacggctatt tgatattact
      121 cattcaatta tcaaatcaca gtaggtgtga atctcatcgg gatcggcaat atctacttct
      181 gattaggatc aaaggtgatt ttctcaataa aggtgtgata caaagcttta acttgcttct
      241 tctcactctg gataagaaag caatccaaac tagtcaatat ctgttggaca tctttgactt
      301 gaatcggttc cccttgttgt tgaaagatcc ctaagatttc attctctcga atttgtaact
      361 gtcttcgttt ttcagtcaat tcatttaaac gatcttttaa catcggaaac aattcgggac
      421 tatcttccaa agcagcttcc tgtttttgct ctaatagtta gattttgcgt accattttct
      481 tcgtttattt tccttcgatt gctgatttta atatagtcta aaggaaattt atatttattg
      541 tattctttat aacttcaaga gaacttcgtt tgagtattac tgtatttttt taattccata
      601 agaaaattag ttattacctg ccacctaatc agcaaatctg aacctgagtc aatataatta
      661 atattatttt ccatctccaa cattgtaagt actgttggtg ttaatatagg gactttgtac
      721 aattgtattt ttttgatttt atcatctgca caaaggagat tttttaaatc taatccttct
      781 tttatttcaa aagttgaatt ttttaaatct ataagagtaa aaccaattcc ttcatactga
      841 atttctgaaa atcgaatttt tctcccagtt gatagatcaa ggggcataaa accttttatt
      901 acattgtgag tgtcaccatc atttacaatg tcattaggaa ttttgatttt agaattctgt
      961 ggagcaccta ttggaagata ataattcatt ataattttgt agtcttcatg aggaaataaa
     1021 gtacttttag gattgctaag atattctcgt accgaatcta agaatggctt atccattgaa
     1081 tcttcatccc catttaaact aaaaaacatt gctacaattg cttttgcaac tctaagtgaa
     1141 ttcattttct ttattgaatg tgttaaatca gacttaccat tctttaaact tgtattgtta
     1201 taaaggaaat ttttattgtc ttctgcaaat ctaatatatt cctcaacata ctcactacca
     1261 aaaaaactat tacatttact acataacgta taataaacat ttccttgagg tttcctttta
     1321 atccccttcg gaaaatcacc ttttgtaaaa tcgctcttaa ggaaatcatc taaattacca
     1381 gttctggtct ttttacctcc agtattccct gattttttag gtggtacatg ttctttagta
     1441 agttcagtgt atttttcaca taaaatacac ttacccatct ttttcatatc tatccccact
     1501 ctttttaatt cttatataca attatatttc aaatattcat taataaatat ccctatttat
     1561 gataaggacc tacataactt tccccctaga tattacgaat atccaggggg tgttattgca
     1621 tgcgtaacag cctatgctaa tttacaacat agatcgaagt ggcttagctc taaaaaataa
     1681 gccgcatctt tgtcagatac gactgtttag cattgctcat tcaattagtt gatgattttt
     1741 tcatcaaatt tcagatatag ttgaatattt cctttgttca gcttatcaaa tgtcacttta
     1801 tcaatgaatg ttcgatagat ttccttgttc tcactcttga aacgattggc taacaacttt
     1861 tcaacacttg ctcctttgtt tcgaaatgga ggctactcat caaaaagaat aaattcacgg
     1921 tactttctag agagtattga tagcaaaata tttaatccaa tcattgaacg acttccttca
     1981 attaaattgc ttggaagctc tcttgtctca taatataaca aaaagtcgga atgctcgctg
     2041 aggaaatttt ttcccagctc agtaactccg caaagtggaa ttcggttcat attcaaattt
     2101 ttgattgttg atttgatgct tggtgtctca ccgcttagag acgtaacgat cacaaaatca
     2161 tcttttgtca aagtatggct taacatttca aagtttgttt cacctgaaat taacatggct
     2221 ggtcgtcccg ataaaatcaa atcgtttaca aagtcttttg aaaagttatt ttgcgtaaag
     2281 cctgtcgcat agacaactac tgtttttgcc gcttttagct gttctaatat cggttggaaa
     2341 tttgtttggt ccgctaattg aaaagttcgg ttgatttcat ctttcaatag tgcgataaga
     2401 tctgtcggtg gaataaccga ttgaacgatg ctctgttcaa gagaatattt catttcagaa
     2461 tacccacgaa atcccaattt ttttgctaaa cgtaagacag aacttttaga agacaataat
     2521 tgttctccca attctacaat ggacatgtga actacttgag ctttatgcgt acgaataaaa
     2581 aaaaccatat ctttttcatt atctgagagc aaatcaaagt ttagtcgaac attttgttca
     2641 aaatccattt cctcacctct catatataca gaaggaattt cggataaaac ttgcgctgtt
     2701 ttatccgaaa ttctttctta ttgcccaaat tctaacacag tttttacgtg cttgctatat
     2761 atgataaaca agtgcttcgt aaggtcgtaa tataaactta tcttccaatt cagatggtga
     2821 ttgctcataa tttgatagaa gaagaacccc ttttcgacct tgcaatgatt taggtaattc
     2881 atagggaaca gactcttttg aaaaagaaca gatcaccact aatagttcat ttttatactc
     2941 acgtatataa ccaaacactg ctgaatcatt ctcattgatc aatttatacg taccatgctg
     3001 aatgattgga tagtttttac gcagtgaaat cagtttttga taatgataaa aaactgaatt
     3061 tggatttttt aaagcatctt ctacattgat ttgttgatta tctggcgaaa aagagatcca
     3121 aggatcactg ttcgagaatc catacccttt tgaatgatcc caaggcattg gtgttcgtgc
     3181 gttatcacga gatttcaaat aaatagcacg tttgatgtac tcatcactct cgccttcttc
     3241 tttcatcttt ttataaaaat agtttgtttc aatgtcttga tactcatcaa ttgaagaaaa
     3301 tggaacattt ttcattccaa tttcttcccc ttgaaaaata aatggggttc ctttcatcat
     3361 gtgaagtagt gttcctaaca tcttactaga ctctatccga tattcgtttt catcaccaaa
     3421 tctagtaaca gctcgcggtt gatcatggtt actccagtaa agagaattcc aaccatctga
     3481 aagcgcttct tgccactcac ttaacgtagc tcttaaatcg ctaagcttaa accttacatc
     3541 tgaaaacttt ccatactttc cataatcaag atgcatatga tcaaaatgaa aaaccatatt
     3601 taattcttta cgctcaggaa acgtataaag ctgtgcctgt ctgctatttg tgttcggtgt
     3661 ttcaccgaca gttaagctgt catatttact caaaacttct cgattcatct cttgtaaaaa
     3721 ttcatgcact cgcggaccgt tggatgctcc gacataataa gactttgtat agggcatgct
     3781 atttggagca tcaggataat caggatgctt gctaatcaag ctgataacat ccattcggaa
     3841 tccatcaatt ccaagatcaa accatttttt catcatttta taaatagact gacgaacttt
     3901 cgggttctcc cagtttaaat ctggttgttc ttttgcaaaa caatgtaagt aatactgctt
     3961 tctactctca acatactccc aagccgaccc gccaaatgtc gatccccaat tcgtaggtgc
     4021 actcccatct tctttgggat ctttccaaat ataataatca gaatatggat tttctttatt
     4081 tttttgtgat tctataaacc aagcgtgctg attacttgta tgattgacga caaggtctaa
     4141 aataactcga atattcagtt gatgcgcctg ttccaataat ctcttaaaat cttctaacgt
     4201 tccataattt tctgaaattt tctcatagtc tcgaatatca tatccgttat caattagcgg
     4261 agagtcaaaa attggagtta gccagataac atcaacccct aactttgcta aataaggtaa
     4321 tttttcaacg atccccataa gatctccgat accatcattg tttgaatcct tgaaactttg
     4381 aggatacact tggtagacaa cactactttt ccaccattca ttcatccgtt actcatcctc
     4441 ctcatcaacg actatatttt ttaagtaatc attgacataa accaattggt cttgtgcttc
     4501 ttggattgcc tcacggatgt ggttgtctgt caatcttttg tttaatgtat tcaataaaat
     4561 tgtcaatacc acaggtagat tcatcccggt aatcagatag acattaggtt gattgatata
     4621 ttttataaat gcttgattga ctgaaccacc tagcatatca gtaaaaatca atatatcagt
     4681 atcctcattg tctcttacca ttacttcgtt aatcttttct tctatagatg agttatcaat
     4741 ataacctgcg attgcagtta tttcaccaac attcggtaaa atatattgga cagtatcttt
     4801 caatcctaca gctaaattat gatgactagc aaaaataatt tttttcattc tttattctcc
     4861 ttaaaccttt aaaataccga aaaatgatag aatcaatgcg aatatgatca ctgaaaaaat
     4921 aatttgaatg accgtcattt tttttgaagc tatcaattta taaacaataa ttgttaaaac
     4981 tgcaggtaag agtgctggca tgattttatc aagaatttct gtttgaatag gtaattttac
     5041 ttttccagat tgaaaaacca ctgcaacatt catttttaca acagatggaa tcaaagctcc
     5101 aacgactgtc aaacccataa ttgaagcaga ttctgtaaat atcgataatt tacttcctaa
     5161 agcagtcacc aactttaacc ctgaagtata cccaatcgaa aaaagtttaa atcggaagaa
     5221 caaaaagaat agattcaaac ataaccaaat aatggcacca gtcggatttc cttctagacc
     5281 catataagct gcaatcgagc ccataatcgt tggataaagg acccaaacca acgtgtctcc
     5341 cacaccagct aaaggaccca tcatcccagt cttaaaactt tgaactgcat ctaatgaact
     5401 gactccatct ttttcttcca ttgctaagct ggcacctaat aataaattcg ccatccaagt
     5461 cgttgtattg aaatatttaa aatggttatt cagagaagct ctgtagtctt catcatttgg
     5521 ataaattttt cttagtgctt tttcaagtcc ataaacaact gccggacctt gttgattttc
     5581 gtaattgaag atattacatg ccataaacat atagcgaaat gctgctctac gtatatcttt
     5641 ctttgataat ttatttcctt cattattcat caaaatcatc tccttcatcg actatattgg
     5701 cagctacagg tgatgccgtc tctgcttttt ttatttctgt cgtgaaaaag taatacgcta
     5761 aagcaaagcc gacgattgaa attcccaaaa taggcatgtt taaataggcg gaaagtacaa
     5821 aaccaatcaa gagcatcgtc atatattttt tcaccggcat aacttgtaga agtaatgcta
     5881 ttccgactac aggcaacatg cttccagcaa tcgataatcc atctgtaaac cacttaggca
     5941 caaaatctaa aatcaatcga acaattctat caccaaaaac aacacacaat gcagttggaa
     6001 tggctgtaga taatccgaaa atcactggtc ctaaccacaa aatctgattc attttcttaa
     6061 aatccatttt atttgctgcc ttttgtgccg catgagtaac atatgtgttc aatattttag
     6121 ccaacacatc taattgaatt cccagcatcc caacaggaac acctactgct aatgcaacag
     6181 cttttgcttc ttcaatatta tttgtggtac ggatggctac gtagatacct acaagagcag
     6241 ctaaacccca gttaggaacg gacgatcccc caatattcgc tacacccaaa gccattaatt
     6301 gaaacgtacc gccaataacc attgcggttg tcatatctcc cataatcagc cctgcaaaca
     6361 aagcaatcat gatgggaaac catgttgtaa tgaccaatcc ttgttgatct aatacaaccc
     6421 acactgctaa aataataatt aaaattcctt ggataatttc cattccaatt atctccttta
     6481 ttttattctc acatctaatg gttcttcagg atctgaagga acaaaatgaa aagtaactgg
     6541 tattcccata gcctctaatt tttttaaatc agcaatttct tgttcatcaa ctgaaaccat
     6601 tttttttact gtttgtttcc cttcaccatc aaacatgatc ccgatattca cttttggtat
     6661 atcgataccg ttctcagcta gttttactaa tgtactcggt tcttttacga tcataagcac
     6721 attatgactg tcatacttgc caagtttgaa attaataata gctgtatctg tatcaataat
     6781 tgacatcccc gttccagctg gcttactcat tcgcattgct gctttttgtg tgtcatcctt
     6841 actgacttga tcatctacga ccatcaatcg ttttgctttt gtcgcacctg cccactgtgt
     6901 taccacaata ccgtgaatca atcgttgatc gattcttgct aatactaatc ccatatctat
     6961 cgtctccttt tttgttatcg tttacatccc aaagtatatc tacgccttgt tactttaaca
     7021 atagttttag aagtatcagg gaaacagtcg ttatcttagg aaatagtttc tttattaaga
     7081 gaaatttccc acaaaaaaat agcccaacca tcgattagat gactgagctc cctaaattta
     7141 cgctatcttt tattactatt tacttctttt ttatcgcaat ccaaatttcg ccatcacaat
     7201 ccgtcaactc ttttgagaaa tcagtaaagg agagttctga tgcttgaacc aactcatatt
     7261 ctgaagttgg caaccactcc gcgtatattg acccactgac atccctaaaa aaggtgcgag
     7321 gtggagaata tttttgatac aactatcctt atttatgata tggagccccc gcattgatcc
     7381 ggaaggcacg gtagatttgc tcgacaagga tcagacgcat caattgatgt ggatacgtca
     7441 tttttccaaa agagatctga gcattgcttc ttttcatgac ggcttcgctg agtcctaaag
     7501 agccgccgat aacaaaaaca aggtggcttt ttccttggat acctaattga tcgatttgtt
     7561 tagcaaaaga ttcacttgta ggatttttcc cttcaattgc taaggcatag acgtattctt
     7621 gttctttgat tttcgctaag atccgttccc cttctttttc ttttacttgt ctcatttgcg
     7681 cttcgcttaa attctccggg gctttttcgt caggaacttc gatcaattcg atttttgcat
     7741 aggcattcag ccgtttgaca tactcattga taccttggat caggtatttt tcttttaatt
     7801 ttcctactga gataattttg atattcatgt tttctctcct ttggtcttca cgctcacttt
     7861 gtttattata atcaattttt tcgtctatat gtttattttg gctatttttg ttaaaaagat
     7921 ttccccaaca gttatgcaca acatccacag gtttatacac agaaaacagt gttattttac
     7981 cttatttcat cacattcatt aggcaaacat acgttttata agaatttatc cactttatac
     8041 acaggctgtg gatatgtttt atcccttatt tttccaatcc tatccgcgta gttttacaca
     8101 gtatctgtgg aaaaagaaaa tcaaatattt tcatctgaaa acaatgattt ttcctaaagt
     8161 ttttcataaa aagttcacag tctcaaagta gactaaagtc aacaaataag actatactaa
     8221 cagtagataa ataaatgtaa ttggaggagg aacacatgga tagaaaaaat gtgacaccaa
     8281 agatgaaaaa aaataaaaat aatagcctct ggcgtaagtt aggtctgggg cttgttggag
     8341 ggatcgttgg aggactactg acagctggca ttttttatgc agcgatgggc actggcaaca
     8401 atagttctac tacaagtggc aatcaaaatt ctgctggtga aacagttgtg gaaaacgtca
     8461 aagtcaatgt agattctgat atcaccaaag cggtagataa agttcaagac gctgtcgttt
     8521 ctgttatcaa cttacaaagc caaaaccaat caagcggttt tggcggacta ttcgggcagc
     8581 aagaagaaag tagcagctca gatgatagta atttggaagc ttatagtgaa ggaagtggcg
     8641 tgatctacaa aaaagatgga aatactgctt atgtagtcac aaacaaccac gtagtagatg
     8701 gacagcaagg tctagaagtc ttgatgaaag acggtacgaa ggtaaaagct gaattagttg
     8761 gaacagatgc ttattcagat ttagcggtgt tgaagatcaa ttcagataaa gtcgaaacgg
     8821 ttgcctcttt tggtgactca agtgctctga aggttggtga acctgcgatt gcgattggtt
     8881 ccccattagg atctgaatat gcgaattctg tcacttcagg aatcatttct tccttgaacc
     8941 gacaagtaac aagtacaaat gaatcgaacg aaacagtcaa tatcaatgcg atccaaacag
     9001 atgcagcaat caaccctgga aattctggtg gtcctttagt gaatattgaa ggacaagtta
     9061 tcggtatcaa ctcaagtaag atcgcaagta cttctgaatc tacttctaac gtaagcgtag
     9121 aaggaatggg atttgctatt ccaagtaacg atgtagtgaa tatcatcaat caactggaaa
     9181 aagacggaaa agtgacacgt ccagctctag gtatcacaat ggtcgactta tctgccgttt
     9241 ctactcagca acaagaacaa atcttgaaga ttccttcatc tgtaactaac ggtgtcatcg
     9301 tgacaaatgt ccaaacagct acaccggctg aaaaagctgg attgaaacaa tacgacgtca
     9361 ttactaagat cgatgacaaa gaagtttctt ctggtgtaga acttcaatct gctttgtata
     9421 agaaaaaagt gggagattct gtcaaaatca cttactatga aggctcgaaa gaaaaaacaa
     9481 ctactgttga attgacgatc gatcaatcgg cattaaaaca aaatcaaaat agtgaaaatt
     9541 aataaaaaag aggttgtgac aaagacttgt cacaacctct ttattttgga aatgcttctc
     9601 cgactacggc atcctcaggc aatactaaga ttcctttttt tgctggagca tttggcaaat
     9661 gcaattcacg agcggaacag atcattccat agctatctac ccctcgcaaa gttcctgacc
     9721 agatcatcat tccatctggc atcatagctc ctggttttgc aacgacaact ttttgtccag
     9781 cttcgatatt gggtgcaccg caaacgattt gcaatactgt accattgtct acttctgtct
     9841 cagtaatcga taaatggtca gaatcaggat gtgggacgca tgtttttacg tacccgacaa
     9901 cgatcttcgg ttcatgatca gctccaagct gttcttcgaa tcctgcttct ttgatcaatc
     9961 gattcaattt cgctaattcc tcatctgtta agcttacttg gccgttccct tcgattgtta
    10021 gatgatcgga gatatggaaa aagttccaag caacaacttg accttgttca tctttgatcc
    10081 gagcaacatt ccatttgcgt tcaacggtgt tttctgttcc tttatcgtcg gctatgataa
    10141 ccatcaaggt gtctccgacg tgtgctttgt tataagcaaa aatcatgttc tttcctccat
    10201 tatcctttgc tgagatttat cttaacaaaa aattgcattc actgcatgag atttttttca
    10261 tgctttattt ccgaagaagc ctcttctgga acaccattta ttcgtaacaa gggactgtga
    10321 caagaatttt gtcacagtcc ctttactgtt ttagttaatt gattaaaaag gtcgtacaag
    10381 ttccacttgc ttaattactt ggctgttctt cttcgtcaga aacgatcaag acatcacaag
    10441 gtgaatgacg tatcacgtag ctggctacac ttcctgtcat aaagcgttcg actgcattta
    10501 gacctgactg cccaaccatg attagatcga tctcatattt ttttggcaat ttgactgcca
    10561 tggattcacg aacagaacca tatgcaataa ttccttctat atttgtgtaa tcaacagatt
    10621 tcccatattc tttacattct tcgatcattt ccttggctgt ttcagtttgc tgatcgacta
    10681 gattttgatc aataggtgca tagcccataa agttataaaa ttgctgatcg attacgtttg
    10741 ctacatatac tttcccatta ttacggcgag ccacttctac cgcttttttg aaagcagcaa
    10801 gggcttgttc actaccgtcg atacctacta aaatattctt atattcttga ttcatcttga
    10861 cccctcctaa cagatgtcag cctttcgtta cactttctag gaacgattcg atttcctgtt
    10921 ttgttttacg atctttattc actagacgac ctaattcttt tccgtcttgg atcacgacaa
    10981 aacttgggat accaaaaatg ttccattcag cggctacatc tatgtacttg tcacggtcta
    11041 cttcaataaa ttgccaatca gaaaagtcat tttcaatttc aggcatttgc ggtttgataa
    11101 aacggcagtc tccgcaccag ccggcagtaa agaaaaatac actctttcca gtttctacgt
    11161 agcctgctaa ttcttctaaa ctatttggga taatcattct ttgtcctcta ctttctgttt
    11221 atgtattttt caattaaatt atacaccttt ctttcgaact aacaaacatt gatcctcttt
    11281 tt
//
LOCUS       DACCSK010000088        11231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCSK010000088 DACCSK010000000
VERSION     DACCSK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.090"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(133..2031)
                     /locus_tag="IVP39_002059"
     CDS             complement(133..2031)
                     /locus_tag="IVP39_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7587123.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2192..3454)
                     /locus_tag="IVP39_002060"
     CDS             complement(2192..3454)
                     /locus_tag="IVP39_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7587124.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(3447..4346)
                     /locus_tag="IVP39_002061"
     CDS             complement(3447..4346)
                     /locus_tag="IVP39_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587125.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(4491..6269)
                     /gene="uvrC"
                     /locus_tag="IVP39_002062"
     CDS             complement(4491..6269)
                     /gene="uvrC"
                     /locus_tag="IVP39_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7587126.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(6451..6765)
                     /gene="trxA"
                     /locus_tag="IVP39_002063"
     CDS             complement(6451..6765)
                     /gene="trxA"
                     /locus_tag="IVP39_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7587127.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(6852..9212)
                     /locus_tag="IVP39_002064"
     CDS             complement(6852..9212)
                     /locus_tag="IVP39_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7587128.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(9327..9875)
                     /locus_tag="IVP39_002065"
     CDS             complement(9327..9875)
                     /locus_tag="IVP39_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7587129.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(9933..10370)
                     /gene="zapA"
                     /locus_tag="IVP39_002066"
     CDS             complement(9933..10370)
                     /gene="zapA"
                     /locus_tag="IVP39_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7587130.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            10572..>11231
                     /gene="rnhC"
                     /locus_tag="IVP39_002067"
     CDS             10572..>11231
                     /gene="rnhC"
                     /locus_tag="IVP39_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7587131.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QE"
BASE COUNT         3169 a         2385 c         1889 g         3788 t
ORIGIN      
        1 tcatgtttgt tgaatagtgt gacttgtgac cgaggagttg gcagaagaac accgtttatc
       61 cggaaaaaga gactgcgaca cgacttttgt ctcagtctcc agtctatgga acaatgttca
      121 ctggaattct tctcaatcgt cctttcgata gtccaacagc cttttgagct gggtaatctg
      181 ctgctgcagt tccatacctt ttgttgtatc acgattgagt ttgcgtggta gttgctgttc
      241 gattactttt ttcacatttg tttggtcgga tttttttgca aattgttgtt caataggcaa
      301 aaacggataa tgtgtcacga tttggaaacc ataagaattg cataatagtg aataaccggc
      361 aattcctgtc gttttttgat aagctttgga taaacctccg tcaatgacaa atacttttcc
      421 atttgctttg atcggtgact cccctttttt tacttttact ggcgtgtgcc cattaatgat
      481 tgctgaccct tcttcttttg tgtcgaattc tgctaaaatt ttttcactga accactcttc
      541 atctcgcaag tgataataag gattgctttt ctcgacgtgc gtatgaggct ctgtgagaaa
      601 ataacgttca aaagtcgcca ttttactctt gccgaataaa ggcgaaaatg gcccactcca
      661 cgcataccaa atcaaatcgg ttgctaaatc ttcttttgca gtcagatctt ttgcgctttc
      721 agttatctgt ttttcaaaaa agcccagcag ttcttttccg cgataagatt ggtctaattc
      781 aaatgctaag agctgtccct tctcgtctac aggtatacag ccatgaaaca gcacatgctg
      841 attgttcgtg agatacattc cccctttttc gatcaaaaac gtcatatgtt tttgcattct
      901 caaagagtgc tggaatgaat acatcaaact atcaatcact ttttgttcct cttccaagaa
      961 tttttttgga tcatctggct ggatcgtttg aaaacaagtt cctttcaact catagagctg
     1021 accttctaaa tcaatcgttg atgcttcata gtcgattttt tccagcaatt tacgatcgtc
     1081 cattttaaat tcaggtcgac gatccaataa ctgatgttct gatttgaatt gaaggataga
     1141 aagtgcctga tggatttttt ctaattcttc ttgctctctt tcggatagat ttttactgtt
     1201 tttaggcgca aatactggat tcttctgata agtttgatga gcaaaagaaa acaatggacg
     1261 taaattcaac ccatattctt tctctaattc aaataaataa ttatagcgtg ctgcaatccg
     1321 taaaagtgtc agtaaacatg cctcagagcc gcaatacgca ccaatccaca aaatatcatg
     1381 atttccccat tggatatcta cagaagaaaa atccatgatt cgatccatca ctttggctgc
     1441 ttgagttcct cggtcaaaaa tatctccaac gatatgcaaa tgatcgatga cagatttttg
     1501 gatcgtctgt gcaagtttcg ttaaaaattt ttcttcttcc ttcatcaaaa caagttgttc
     1561 aatgattttt tttacatagt ctttttttcc attgatacgg tcatcgatat acaagagttc
     1621 ctcaatgata tagctatatt ctttcggcaa tccttttcga acttttgacc gggtatattt
     1681 gactgtcgta aaccgtaaaa gatcgatcaa atgatggatc agctgcgatt tatcttgttc
     1741 tttgtaccaa tcagtcgtta atgcatattc tggataagcc gtaaaaatag tgaattggct
     1801 gatatccgtt tccgtcattt gttcagaaaa aaggtctttg atcttttctt taagatttcc
     1861 tgaaccgata cgcagaatat gatcaaaagc aggaaattcg ccatgtatat cactcaaaaa
     1921 taactctgtt ccttttggaa gctgcaaaat tgcttcgaga ttgatgattt ccgtgatgac
     1981 atcctccgtt gaggggaatt tttctgaaag taaatctaaa taacggtcca ttaaaacgcc
     2041 tccaaaaatt ggtatactca atattataaa gttgttatac actattttca atgaatatat
     2101 atccaatata gtaaaataaa taaaataggt ctatacaaaa agaagctggg aacaatagat
     2161 gtccagcttc cttctgtatt ttaggattgt tttatgcttt ttttcgttca ttcctcataa
     2221 ttgaaatcga ttgtttcaat gaactccata atccgccttc gctataaacg agtacattcg
     2281 atttgtacat gtttgcagac aagatcatca ataagaatgt tacggctaca agtaccaaca
     2341 aggaaatcaa tgctccaccc ataccgactg tctcatttgc taagcgaatc ggcataatat
     2401 atgaagataa gaatggaata tacgaagtga tccgaataac gatattgttt ggatctgaag
     2461 ctcctagtat caatccaagc atgtatccgc ctagagatag ataagtcact ggcaaaattg
     2521 cttttgctgt atcttctgct ttgttgacca aagagccaca taaagctgct aaaacagaaa
     2581 aaatcaaaat gccaaagatc atgaatagaa gagaatacca caagaatggt ccaatcaagt
     2641 tatccaatga gataccgtct aatatatttt tgaccatttc catatctttg aattgactgt
     2701 atcctatacc aaaaataata ccgtataatg ccatttgcgt taatgcaaca agtaaaacac
     2761 ctgttaattt tccgtaaaaa tgcttttgtg cagtcgtact ggacaggatc acttccatga
     2821 tccgcgtgcc tttttcagaa gcgatctctt gtgcaatgat ctgcgcatac gtcaaaataa
     2881 taatgaacaa gacaatcgtt gctacataac ttacgacgta ttggactgtc gagttatctt
     2941 ctccgactgt catttttcct tttgaatcaa aattgacttt ttgtcgagtc aaacttgctt
     3001 gttggctcaa gctagctact tcttctggtg aaatgcctaa ttgactcgct cgaagcatcg
     3061 attgcaagcc agtcagttgt tgttgtatca gtagttgtgt actttgtcct aatgaatttt
     3121 cgctataaag catcccattt atcttatcat tctcagttga gactactaaa tacgcatcga
     3181 ttttttcttc cgaaagctct ttttgtgctt tttcttcaga atcaactgct ttgaactcaa
     3241 aatcatctgt tttgacttgg ctcatttgct gactgatttg ctcattatca gccaatacgc
     3301 cgattttatt gacatcacta ttttgttgag caaagtttcc tgctacataa atgataccca
     3361 gcatgataaa agggactaat atcatgatca agaaagaaat cgacttaaca ttttttaaat
     3421 agacatcttt ggtaataatc caaaacttac gcatcgattt cacctgcttt caatttgaaa
     3481 atctcttcca aagttggcgg ttgctggtta aacataggaa tgtagccgaa ctgagtcgca
     3541 cgagtaaaga tttctttccc agcttcagga tcttctaagg taatctctaa cgtattgtct
     3601 tcatgaagga tcactttttt cacgccttcg atttcttcta cttcctgttg gcttaaacca
     3661 gattccaaga ataacttcgt acggccaaat gattcacgaa tctcatgtac ttttccattc
     3721 aaaaccattt ttccgtttcg caacatgatc aaatgatcgc agattttttc tacattatcc
     3781 atattgtggc tagaaaaaat gacacatgaa ccatgttttt tcaattccaa tattccatct
     3841 tttagcaatt cagcatttac cggatctaac ccgctgaatg gctcatccaa aatgatcaat
     3901 ttaggttcat ggattaacgt ggcgatcagc tggacttttt gctgatttcc tttagacaaa
     3961 gattttactt tatccgtctt tttccctttt acctgaaatt tttccatcca aaagtcgatt
     4021 tttggttcaa tctcttttct tgatttccct ctaagttctg caaaataaag cagttggtct
     4081 tgaatcgtta ctttcggata aagtccccgt tcctccggaa gataaccgat gacatcataa
     4141 tctttctccc caagatgatg gccattccat aacacttctc cttggtcttg tgtcaaaaaa
     4201 tccaagatca aacgaaaagt cgttgtcttc ccagccccgt tttggccaat tagccccatg
     4261 atttttccat cggggatttg gaaagagaca tgatcgacag ctgtgtagtt cccaaaagtt
     4321 ttaacaagat ccttgacttc taacatttct tcactccttc ttcaagaaat cacatatatc
     4381 cttagacacc agacgtaagc gatttattat ttaccaacaa aacgtaaacg ctctttttgt
     4441 tgtacataaa atgataggac actttacttt ctttgtaaag tgccctttcc tcattcctga
     4501 tgtaatttat caaatacgtt ttgtgcgaca tttttcggca gtccgatttc ttgtaattct
     4561 tctgtcgtgg ctgctgtgat atttttcaac gatttgaact ctttaagcag catttttttt
     4621 cgttttggtc ctaacccttc aatttcatct aatcgggaag caaaactgtt tttactgcgt
     4681 agctgccgat ggaaagtgat tgcgaatcga tggacttcat cttggattcg ctggagtaag
     4741 aaaaactctg gtgaatttcg ctcaagcggt actacagcca aatctgtacc gaatagcaac
     4801 tcatttgttt tatgtttatc gttttttacc atgccagcaa taggaatatc gattcctaat
     4861 tgattttcta agacatcttt tgccacatct acttgtcctt ttcctccatc gatcaagatc
     4921 aaatcaggta aaggcaaccc ttcgcgaatc actcgcgaat atctgcgata gatgacttct
     4981 ctcattgatg cataatcatc cggtccgttg actgttttta ttttatattt ccgatattct
     5041 tttcttgccg gttttccatc aatgaatacg accatcgcag ccacaggatt cgtccccatg
     5101 atattagagt tatcaaaaga ctcgatacgt attggggtcg ggatattcat cgcgtttcct
     5161 aaacgttcaa ctgctccgat cgtccgttcc tgttttctgg caatcagatc aaaccgttca
     5221 ttcaaagcaa ctgaggcatt cttactcgcc aattcaacta attttttctt ttctcctcgt
     5281 tttggttgca gcactttcgt agctaacaaa gcttctacac tgggcttgtc gatcgtatca
     5341 ggaatcaaga cttctttagg aatgaaatgc tcattttcct gataaaactg accgaggtaa
     5401 gtcaagaaat cttcttctgc ttcgttatag aaaggaaaca ttgaaacatc tcgttcaatt
     5461 agctttcctt gtcttacgaa aaacacttgg acacacatcc atcctttatc aatcgcataa
     5521 ccaaatacat ctcgatccag taaatcagta tttgtcattt tttggcgagt catgattgtc
     5581 tcaatggcac ggatctggtc tcggtattct gcagcttttt caaactccat gttttcagca
     5641 gctttttcca ttttctgatg gatctctgac tcgatttttt cgtgtcctcc gtttaaaaag
     5701 tttttcactt catcaacgat tttcgtatag actttcggat ctacgtcgaa ataataagga
     5761 cacaggcatt gtcccaaatg atagtaaaga caaggctcct tcgtctgact aggtttgcat
     5821 tttctcagag gaaataatcg atcgaggatc ttctttgtct cattcgccgc accgacatcg
     5881 ggatatggtc caaaatacag tgctttgtct tttagtacct tacgagtaat caccaaacgg
     5941 ggatattttt cattcgttat cttaaggaaa ggataggttt tatcatcctt taacataatg
     6001 ttgtatttag gatcattttt ctttatcaga ttgatctcta agagcaatgc ttcgatattc
     6061 gattctgtaa cgatgtattc aaagtcttcg atttcactaa caagccgttc cgttttcgta
     6121 ttatggctac cagtaaaata tgagcggaca cgatttttta atattttggc tttgccgaca
     6181 taaatgatcg ttccattttt atctttcatc aagtaacagc cgggctgatc aggaagtaaa
     6241 gctaatttgt ttttgattcg ttcgttcatc tggtactccc cttcatattc aattaaagtt
     6301 attatagcat gtctgtcaaa aaaacaatcc agcctgacaa cgtaattata ctcaaaaaat
     6361 attaatttga ataaccttaa ctcaaccgca aaaaaaccga ggacggaact ggattcagtc
     6421 ccaccctcag cttatttttg ctgtaagttt ttataaatat tgatcgatca tttgacgtag
     6481 ttgttctttt gaatgaacac caacagcttt ttctactact tgaccatctt ttttcaacat
     6541 taatgttgga atgctcatga taccgaattg ttgaggagtt tctggatttt catccacatc
     6601 cattttagca attctgaatt cttcttcatc atattcttgt tccaactgat ccaaaatcgg
     6661 tgcttgcata cgacatggac cacaccaagt tgcccaaaaa tcaatcaata caagtccttt
     6721 atctgtttct tcattaaatg tcttatctgt aattacttgt gacatatcaa agacctccat
     6781 ttttaaaatc tatgactagt atagcatttc tatacttgga tagcgaacca tctgcttacc
     6841 tttagtaagc tttacttaaa tttaacaatt gtcgcaccat ttccgccttg gtttgccggt
     6901 gcaaactcaa agcttttgac gctccgatgg tttttaagat aatcagtaat tcccttgcga
     6961 agtgcgcctg ttccttttcc gtggacaata gtcacttgag gataaccagc tagaatggca
     7021 gaatccaaat attgatctac ctcacttagc gcttcttcgt atcgttttcc tcgaaggtcc
     7081 aattgattcg gcacgtgact actagattca gatcgtacag catggataac tcgttgtgtc
     7141 ggctcttttt gaggagcaac aggcgtcagc tcgtcttcgg acacattcat ttttaatata
     7201 ccgatttcta cttgccactg attcccgttt ttacgtaaaa gcgtccctct ttggccgtaa
     7261 gtcgtaacca acacttcgtc tcccggtttc aacgtttttt gttctttcgc cttcttcaat
     7321 actttatttt tcttcaaagt ttcttcttga tgtagatttg ctaattgggt ctttgcatcg
     7381 atcaactgat gctctttcac atttccttgc ccaatctgct gttgcatttt acgaatatca
     7441 gcaatgattt tttctgcttt ttcttccgcc tctgacacga cttcatttgc ttttttcttt
     7501 gctttttcca tttctttttc acgttcttca aagaaatagc tgtaagcttc tttcaaatca
     7561 tcgtgaagtt cttgtgcctc agaaacaaaa tgacgcattt ccagatattc ggtctctgcc
     7621 attttccggc gattctctaa atctgtgatc atttcgttta aatcttggct ctcatcattc
     7681 atcaactgct tcgcttcatc aataacttct gtatcaagac ctaatcggct agagatttca
     7741 aacgcattgc ttcgtccagg aacgccaatc agcaaacgat acgttggact taatgtatcc
     7801 acatcaaact ccatgctggc attaatcgta tttgcccgat tatatccata aactttcaat
     7861 tcaggataat gcgttgttgc catcacataa gcagactttt taccaagatc atctaagatt
     7921 gcgattgcta gagcagcacc ttcttgcgga tctgttcctg caccgagctc gtcaaataat
     7981 accagacttt tttcattgac ttttgataaa atatctacag tgttcgtcat atgagaagaa
     8041 aaggtcgata gactttgctc aatcgactgt tcgtctccta tatccgcaaa tacttcctca
     8101 aaaataccga tttgactttc ttcacctgca ggaatcggta atcctgcttg ccccatcagt
     8161 tgtaacagac ctaaagtctt caacgtgatc gttttcccac ctgtgttagg tcctgtaatg
     8221 acgatggctt gatagtcttt tccgattgtg atgtcattag aaactacttt ttcctgatcg
     8281 atcagtggat gtcttgcttg tttcaagacg acatgattgt cttcactgat tcccggcacg
     8341 atggctttta cttctttacc aaatcgtgct tttgcattca tcagatccat tttaccaatc
     8401 acataagtat tgtggatgat ttcttgacga tggggaacaa gttcagcaga aagttcactt
     8461 aaaatacgtt gaatctcatt tcgttcagcg atttggtatt gacgcaaacg attattcaaa
     8521 tccaccactt gttttggttc aataaataaa gtctgacctg aagcactttg atcgtggact
     8581 acaccgccaa atacaccgcg gtattcttgt ttgactggga taacgtagcg gtcatttcgc
     8641 atggtgatga tcgcatcact taaatacttt gcatttttcc cacggacgat cccatctaac
     8701 tgttcacgaa cagcttgttc acttcgccga atgttctgtc ttatgctctt taactcagga
     8761 gaagcgtcat cagtcacgcg tccgtcttca tcgattgctt cctttaaacg gcggctcaaa
     8821 acagggatcg tgactaactg atctgcccac atatacaaac gtgcaaattc gatttcactg
     8881 tcagaaagat catcaatgaa acgattcaat tcgcttgtcg tagacaatac tctggaaact
     8941 tgtgcaagct ccactccatt caagtctgct ccgatctcaa tccgtttcat atggggacgg
     9001 atattttcaa ttttaggtat agggatcccg cctcgcagac gttgcacttt caatccatcc
     9061 tcagtttctt gaagccagtt tgtgatcgtt tgtttctcac tcactggtac caattctgcc
     9121 agttcttctt ttccttgtgc agtcactaca aattgtctga ccatctgttt gactttttca
     9181 aattccaatg tttcaaggat tcggttattc attttatcac cttatctttc actatgaaaa
     9241 aagagctgtg ccggacatac gctacgcagc cttcttaaat aaactactct gtacaaaaaa
     9301 cgaaatattt tccagcacta ttttccttaa ttcaaaatat tcgttaccca aagctcatac
     9361 atcttgttgg taagaaatgg cgttctctca acgataaaac gagcgaaact atgctcgccg
     9421 aattgatttt ggaccacatc taatggaaca aaggcaagta actgcaaaat caaaaatata
     9481 gtaacgtaag ccatgattag tgacaggatg cctcctgcaa tccagtctac ctgtctcaag
     9541 atcggcacat acgtcaatcc atggacaaaa acccccacaa atcgggtaag cagccagcca
     9601 attagcaaaa taatcagaaa agcagttcct gcataaaaag cttcatctag gtgaaaggca
     9661 aatacctgat caaaaaatgc taactttgat gttggggtga cagctgggta tggaatgtat
     9721 aattccaaat gacttgctaa tggtttgtaa aaatgctggg cagcaataaa tgacaataca
     9781 tatccaatcg cgaatatgat ttgcaaagca aacccgcgac gtgctccaga aaaaaacgca
     9841 attagcaata aaaataaaat cagcaaactc agcatgctac gttcctttct cttctaactt
     9901 ttttaaattt ttcttatttt gataaaagga tcttaattag aagatttttg tttaatttgt
     9961 ccttttcgtt tctcgtttaa aatctgttgt gcttccacat gatttttaat ggtatgatct
    10021 gcttgaccat tttctttcaa tacttcccga gcttcgttct cgattgcttc aatgcgtttc
    10081 acacgatttt caagctctgt aaatttgatc atttttttct ttaattctgc tgtttcttct
    10141 tccagctcca atattctttc ttgtttcttc aattgatccg aaagcgcatt gacagccatc
    10201 aagattgctg cttgttcatt atccatttgt ggtgataatt gtttcaattc tgctaactgt
    10261 tcattgatca gcttcgttac gatatccata tgatgcttgg tttcccgtcc gataatagta
    10321 tatgtttggt tggcaatcac cgctttgtag cgtgtttttt cgtgcgccat acgaatcatt
    10381 tcctcctatt tgatgaaagt tttatttagt gtaccatgaa gttggttctt tcaccgttct
    10441 ccacaaatat ttttcacata cttaactatt atatgataaa ctttaaaaaa atgctatgtc
    10501 tcgcaatctc ttagaaaacg aaaagtcgtg agttgcggta tagttttatg tataaaaacg
    10561 aggagaattt tatgagcaac gtcattatca aagcaacaaa cgaccaactt agtcaaatga
    10621 aagcatacta tcgctcttat ttgttaaata aacagattcc ttatactagc tttgctgcaa
    10681 aaaaagacgg gacgaccatt accgcttata catccggaaa agtgatgttt caaggtacga
    10741 atgcagcaat agaagctgat aaatgggggg aggcacagga taaacaagcc aagccagcaa
    10801 aaacagcctc ttctttgcca aaaaatatca gtcaattatc cgttttaggt agtgatgagg
    10861 tcggaaatgg cagctatttc ggtccgctaa cagtatgtgc tgcttatgtg aaacgtgatc
    10921 agcttgcaga attacgtaaa cttggtgtta gagattcaaa agagttaaaa gatagccaaa
    10981 tcatccagct tgctgacatc ttaaaaaaaa ccatccccta taaactgctt gtcttatctc
    11041 cagaaaaata caatgagatc caaccaaaat acaatgccgt tcggatgaaa gtcgctttgc
    11101 acaatcaagc gatccatttg ctgctaaaaa aaatcgagcc agaaaaacca gaagcgattt
    11161 tgatcgatca gtttacgccg gaagcaaatt ttaaaaaata tgcgcgttta gaaaaaaatc
    11221 aattacaaga a
//
LOCUS       DACCSK010000089        10947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCSK010000089 DACCSK010000000
VERSION     DACCSK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.091"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(112..1023)
                     /locus_tag="IVP39_002068"
     CDS             complement(112..1023)
                     /locus_tag="IVP39_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7587132.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1424..2002)
                     /locus_tag="IVP39_002069"
     CDS             complement(1424..2002)
                     /locus_tag="IVP39_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7587133.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2022..2690)
                     /locus_tag="IVP39_002070"
     CDS             complement(2022..2690)
                     /locus_tag="IVP39_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587134.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2705..3775)
                     /locus_tag="IVP39_002071"
     CDS             complement(2705..3775)
                     /locus_tag="IVP39_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7587135.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3946..5310)
                     /locus_tag="IVP39_002072"
     CDS             complement(3946..5310)
                     /locus_tag="IVP39_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7587136.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5307..6866)
                     /locus_tag="IVP39_002073"
     CDS             complement(5307..6866)
                     /locus_tag="IVP39_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587137.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6922..9402)
                     /locus_tag="IVP39_002074"
     CDS             complement(6922..9402)
                     /locus_tag="IVP39_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587138.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(9386..9721)
                     /locus_tag="IVP39_002075"
     CDS             complement(9386..9721)
                     /locus_tag="IVP39_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587139.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9735..10769)
                     /locus_tag="IVP39_002076"
     CDS             complement(9735..10769)
                     /locus_tag="IVP39_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7587140.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(10859..>10947)
                     /locus_tag="IVP39_002077"
     CDS             complement(10859..>10947)
                     /locus_tag="IVP39_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7587141.1"
                     /translation="NITLKVPKAAAGTFTADLTWNLVAGPVA"
BASE COUNT         3148 a         2223 c         1884 g         3692 t
ORIGIN      
        1 gcaggctcac gacctcttcc ttaaacggtg tttaaaagct tgcatctgcg ataataagcc
       61 cggccaaacg aaaaatatga ggagctattt ttgtttggct gggcttattt attactgaaa
      121 ataagtggtc aatgcttgtg tcaaacttgc agcaatctgc tgctgataac tgtctgttac
      181 taattccttt aaatcactat cattattcat ataacccatc tcgattaaaa tagaaggttg
      241 aagattttct cgaagaactt gatagttccc agtggagatt cctcttgaat ccagaggcag
      301 atcagtgaag gagtcagcaa ttgtctgtgc taatgtttcc tcttgtccat agtaataata
      361 tgtcgtgatt ccagttgctt cattcgcata ttccgtagag tctgcgtgta aactgataaa
      421 gacatctgcc tttttttctt cactgtagtc gcatatatct ccaagctgta ctaattcatc
      481 tgtagtatgg gtcaaataaa ccgttgctcc agcatcctct agcgcctttt ttacttttat
      541 ggctgtactt aatgttatat ctttttcttc tgtttgttca tcattgctta atgccccagt
      601 atcatttccg ccatgcccag gatctaatac aatcactgca tcagatagct cttccggttt
      661 tgtttgtata ttctttgtga ctttggttcc atcttcgagg gctaaatatc cagtatagcc
      721 atcatttgtc ttgatctgat aagcattttt tgtttcagaa agaaactccg ccagctctcc
      781 gccgtttata gttgcaagtg gggtagttag cgagtcatct gtatatagtg tacggtcatt
      841 ttttccgatt ttgattaatg tctcttgttc tgtatcatca aatgattcct ttgtctttgt
      901 aaccgtactg ttcgtactgc tgaccgtttg tccaactatc tttttttgac caaaaacatc
      961 tatttgaaaa cagataataa tgataatgat tgcaaataca aaagctccaa ttattttttt
     1021 cattcttgac tctcacctcg tagatccaga ctaagggcca aaacttaaaa tcagcttaag
     1081 taagcaaaaa aggggctgta aaagatataa ccgtagaaaa taacattact gctaaaatga
     1141 tttttttcat ttttggcata tcctgaaatc tccttttgac tattcgtggt gattcatcta
     1201 cccaataaat agtttccttc attctacaaa caattaattt cttcgttatt aaattaaaac
     1261 acttcctgga acaccgacat aataaataaa aatataccct gataatcatg agagaataac
     1321 aaatttgtat ttatgaaaaa ctacctatat aaagaagcaa gatataacga tcttcgtcat
     1381 atcttgcttc ttcacctaac tattttctcc ctatataaca gctttacgct tctcctaata
     1441 caccattcaa atataaatcg atcaattgcg ccacatttag cggttcttcc acaggtcttt
     1501 tcgaccgagt gatgtaaggt gctaacacta agaaaaaatg ccttgctgcg atttcttttg
     1561 aaatcccatt tcttagcaca gattggtttg ctgaaaagat ttcttcaaaa ggttcacgat
     1621 aatttttctt ccatagaaag aaaagatgat ctcttgtttc ctttgaaaga ttgctttcaa
     1681 gatcgtgaag catcagaaac aaatcgacca gatgtgtctc aatcaaaaac tgcgccattt
     1741 ttgtcaaggt tacttgaaat cccgtttcgc ttgtttgaac gatttctctc aaagcttttc
     1801 caacatcttt taagtggttt tctaaaactg ccgtatatag tttttcttta tcaccgaaat
     1861 aatgatagag attgggctgg gtgatcccaa ctttttgtgc gatttctctt gttgaagtgc
     1921 cgagatatcc cttggacata aacaagtcag tcgcttcttt caaaattttt tgctgtgtat
     1981 ctttgttgta tcgatggacc atttttccac ctgctttttg gttattcttt ttctttcaat
     2041 acaccatctt ccatcataaa tattctatcg catttgtcaa tcagccgtgt atcgtgagta
     2101 accatgatcg ttgctttatt tttgtctttt gattcttttg ccaagatatc gactacttct
     2161 cgtgcctttt ctgaatccaa actagcagtt ggttcatctg ctagaataat ggacggatca
     2221 ttgtacaatg cacgagcgat tgcgaccctt tgccgttctc caccagatag ttcttctgga
     2281 tacttgttga tcaacttatc cacgccaagc tgctcaaata attcgtttgc ttctttttga
     2341 cgatgttcat gtttaatctt atctactaat aataactgct tttttactgt cagaaaagga
     2401 accagattgg aagcttgcaa aataaagcca atttctttaa aacgtagttt aacgcgttcg
     2461 ctttcttttt gattactgaa ttcttgatca ttgataaata cagagccact gctaggcgtc
     2521 tgcaatccgc ctgccaaagt caaaaaagta cttttacctg aaccagaagg accgatgatt
     2581 gcaacaaatt cccctttatc gatactcatg gttgtttctt tcaatgcttt aactacttgg
     2641 tcgccatctt ggaaactttt ttctacttct ttaaattcta ttgctttcat gttcttcctc
     2701 cttcttatcc aattgccttc aacggatcga tttttacgat tgtccgaaca gaaaatagtg
     2761 cgcctaacac agctactact aacatcaaca agctaatgac gccaaaaaac agccaattgc
     2821 tttggaatgg aaccgcatca ggtagaacaa gggatgttcc cactgttcct aatagaccaa
     2881 gaagaatacc aacaaatgat aaaatgaatg tttgtgccac tacagaacgc gcaataaatc
     2941 ctcctgtgat cccttgtgct ttcattacac caaagatatt aatcttttgc atcgtcagta
     3001 cataaatgaa aatcccgatc acaatagccg caataacgat caaaaatccg atcataaatc
     3061 caaaagtcaa tacttgcgca ttatatccag gtaattcatt gatgaattta gaaattttga
     3121 tctgttctaa atcttcaggt aaattactga ttttacctct gacaactatt gcgttaatcc
     3181 gtgcattttc acttgtatct tctttttcga agcggatttc ttggtaagca ttgatggttg
     3241 tatagagaac tggtgagaca ttgaatttgg catgatccgt aaagccagtt agtttaaacg
     3301 tcttgtcact accagataac ttaactgtat ctcctatggc tagaccatat tcttctttca
     3361 aactaatatc gcctactgcc tcatcatcgt tatcaaaagc ttttccttca acaagattgg
     3421 gcatgatgaa ttgattttta tcgataccga agaagctaac attgattttt ccttcttctg
     3481 tactgtcttt actagtcacg actccaggtg tttgggcaag atacgccact ttcccacctt
     3541 cgacttcttc tgctatcttc ttagtaatca tggacatccc tagatttgaa tttgcatctt
     3601 tcgaaagcac aatcgaatct gcctcccact tgtcgactgc tgttcgatta tcttgagcta
     3661 agccataagc taacccagtc aaaaaaaata ccaggtaggc gatcaaaaac atcacccctg
     3721 caactaacgc ataacgtaac tttgaatgca taatttcatt taatgctaaa aacatttaac
     3781 tcctcctcct gaaataatca ttttatacaa caattttatc acttgataaa tttttggcga
     3841 atattatgct caaaaaattc ttccgctatt tcattatatg aattttttat tctcgattga
     3901 ttaatattta ttatatcccc acacaataaa agaccgtgat atttctcaca gtcttttatc
     3961 tactctattg ctatttgctc tcttagctag tactttaaaa gctaactcat tgaggtagtg
     4021 aaaacgagag gtgtattcag acagaaatcc aaaatcctca actgctagct gtggaatcgt
     4081 agttttgtgt ttcacgatat ccgcaagcag gttcaaatct gataataaga attcttggct
     4141 tatcagctgt ccgccaatta tttctaatgt atccggatgg tagatcagct ttgcatttac
     4201 tgttttgttt tcttcgtcgt attgtggaaa tagatcaaat tctcctgaac acgaaccggt
     4261 attttgttcc aagaatggcg cttcatcttc tgtcagccct acagttccta aaaagtagtc
     4321 tataatgact gctgctgtta tcttttgtct gaggtcgtaa gcggttggct gaccactcaa
     4381 agttagcgct actgctcttg ccatatgtac agctcgagta actaaaggta aaaagagatg
     4441 ccgtttattt ttgtcaaaac tgactgggac gatatctcca atcgcataga tgtccggatc
     4501 acttgtctgc agatattcat tgactatcac agtctcatcc tcatacaaag ccactttttc
     4561 ttttaacaat gtcgtattag gacgagtttg ggcagaaaaa atgaccatat ctgcctttat
     4621 tgtttgagac agaagttgta cggatactac tttctcttct gattttttcc aatcgatcaa
     4681 gaattcattt ggataaaaat ttatttgctt agtttgcagc tgattttgaa aactgttgat
     4741 catatcctta tcgaaatatc ttgataaaac attcggcata taatcaacta gatgaataat
     4801 gtatccttta tttctcaaag catctgctaa ttccaatcca atgtaaccag caccgaagat
     4861 cactacttct tttgcttctt ttaaagcggc aagtacttgc ttagattgca ctagattttt
     4921 ataggtgtag acattttcag cttcaatttg tttatacaag gtgacaggag aggaacctgt
     4981 cgctacgatt aatttatcat aggttatggt ctcgtttttt ccttctttgg atgcgaggat
     5041 gagctgatgt ttgtctggat caagttcagt tactctccat tcggtgatca gttttgcccc
     5101 actttcttga agagtccgca tgttatgggc aacttcagct aaactacgta tctttccttt
     5161 taaataaagg ttgattccat ttgaaacata gcctagtcca tctttatgat cttcatcgat
     5221 cagtaaaact tctgctttcg gttgaagttt tcttagattt agggcagctg tgatgccttc
     5281 atgggaagca ccaatgatca cgattttcat gttttattca tccttaaatt caatttttct
     5341 aaataacatt cttttaaaaa ttggttcaaa taggggaaat cataattata ttcgttgcta
     5401 gtgaatacaa aaactttagc ggacttctgt gacgggtact ctttcaattg gctagtcaaa
     5461 atgatatcat actgcttttc tttctcgtat tcgactaaat catatttgat cgtattcagc
     5521 aattgttcct ggatgatttc tttcacgata tccgccatta gcctttcata aggaagatca
     5581 taagcaattt gcagacgtaa ttctaactgc ttgatcagcg tccgtaaaat agatgcatag
     5641 cgagcaaata attcaaagcg ggcattttcc tcgtctactt cagtaaaatc aacaatagca
     5701 aatgtttgtc tgaccaatct ttcacataaa accaggaatt gtttatcttc tacttcagtt
     5761 aaccgctgtt ttaatccttc ttggccaaaa atattcatcc atcctctaaa ataggcaaaa
     5821 tgagcgtgat tttgcatcac tgtcaacgca atcttttgcg aaatctcttc tggaaaaaca
     5881 taaaaagaga aatattcttc tatcgattgt ttaaacgttc gattcaattg agctactacg
     5941 tcgtctttgt atttttcgct tatcaaaaac tcctggacat gcgagttatg tgtatctaat
     6001 gtaaagttac tgatcatgaa aagataaaaa accaacgttt cattttgatt ccaaggaaag
     6061 gcatattgaa agaagtaacg ggtcaaaaat gtatggaggt ccaataaaaa ttgtgtcgtt
     6121 tgaaacaatc caacaggaag attgctataa tctggtttgg attttttcgt acagcgcatt
     6181 cgcttcttca taatggaaag ccatatttca aatttcaatc gtccttcttc tgtaatagca
     6241 aaactaaact ctttttgcag cagatctaaa aaagtgtttc ctgataaatc actgtattct
     6301 tttttgtttt caagataggg acgtccaaac caaaataatt ggaaataaaa ataacgaatt
     6361 tgactttctg gaccggtcag ccgcccgctt tttattttca gatgaaactc atttaaagaa
     6421 tcatttaatt ccttcactct gcgagacaaa gtagccgaac tgatattaag ttccatgaca
     6481 agtttctgcc gatcaaatga gccattttcc aacagataaa gaagtatctg atattttgta
     6541 gaatagcgca agtaacaatg ataataaaca tcgactgctg ctatatttcc atgtctgatt
     6601 aataaaatat tcccttcttg gataacaatt tccaattgtt tgatattttc caagttagct
     6661 tgtaaataaa acagatcttc tttcaaggta gcccgatcaa ctgataagcg ggtaagtacg
     6721 ttattaacgg aaacctctcc tttttcctct aataaaagtt ggaaaagttt gatttgccgt
     6781 tgttcatttt tttcaagcaa actgaaaagt tccaagtcca ctccttcaag catttctgat
     6841 tttaacagca gaagtttcct tctcatcctt gaaaaagagg atctgtgaca gttatcacag
     6901 atccatacaa attctaatca cttagctggt tggcccagct gtcagttccc atgtgaccat
     6961 gccttgatac gttgcgccag gttgagcatt cgtactgcta tttacctcta agagtaaacc
     7021 atcggtatcg ggaaagtcaa cgagaacctc tcctttagct gttccatcac tctcataaat
     7081 caacgtgttt tgaccttgct gaatcaattg ctttgatcca gaatgattga agtataattt
     7141 tgttccgctt aattcttgac cttgatcatt cttaaataaa gaagtcaatg tacctgtcag
     7201 ttgccaagga gacgtgcttg gaagccgagt atcccgcaca tcaaaagaaa aatcgccttt
     7261 tgattcataa taacttgtct tcaacggttt ggggagatcg ttaccaaatg taaaatcatc
     7321 tggtacatta actaatgtta attcttgttt ggggacaaga attgttttag cagatgtttg
     7381 gacaactggg ttaggaagct tagcaccatc tgctaatgta atattgctgg aaagtacagc
     7441 tgcctttgtc agtatgctga cggattgtaa agaagtatct acagtattta attgatagtt
     7501 gtaagtaaga tgaatggtat ctcctatttt gctcaataga tctgctggga cagaaactcc
     7561 ttgttgaatg ccggtatctg cgatagttgt accatttaat tttaaggaac cattgatata
     7621 agtagtattt tcaggaagtg agatcgtgat ttttttgtcg gttatcgcat ccccagattt
     7681 tgttaacgtg aatgtcacat tttcttgaat cgtatctcct gatagagcag acaggggatc
     7741 tcctgtctca ggttgtctgc taaggttttt tagctgacta tctgcactca cagatggaaa
     7801 attagccact gttatttgag gaagagtggt gattccagct tgttcagact gccatttgta
     7861 cgcaaaagac ggatacatgt tagtaagtac atcgtccacc aagcgataat caaaataata
     7921 ttgccctgaa gccaatgcag gtagtgtaga agtagctgta tttacacccc cgttatttga
     7981 ttggatcgtt tggaaaagtt gatctgtcca tcctgtcgct aatttttggg ttcctttcga
     8041 ataaagcctg taatggatcg aacccttttt tagttcagtt gctgaccaat tggccgtgac
     8101 ttggtacgat gcagaagaaa ggagctttcc ccactgacta tcggctgtta agtttgttgg
     8161 taaaaccgtt ccgtctagct tgtactgctc caataccttt aaagcaacag gagaaagcaa
     8221 tccgccttgc gcatctttcc cactgtacac gatattggct gtctgattca atccacttgt
     8281 gcttgctgga gtatgagtca gtattgctcc ttgtgtaacg ttttttcctg taaaatagtt
     8341 gactacgcga tcaaagcgac taaatggcaa ctgatcaaaa cgtccttcag tcagccccat
     8401 cgtcaatgtc cgactattgg tagagcctgg aaggttctgg ttatttccat ccacgtattc
     8461 tgtggtatca gcaaatgtat tatattttcc tgttgaatca cttggtgaga tgtaactatt
     8521 ccacgtattt atctctgcac ttgcagaaaa tgcatatgct cctggattca agtttccgcc
     8581 agttaattta ggataaaatc tacctgcagc tggagctaaa atcgaattga acatacgatc
     8641 ttggataata ttattcgaat cataaaaatc aacagaaaaa acggcctttt tagacataga
     8701 aggttgtaat ttacccttgg tcaaaatttt gtttggactc gtatcatatt tatctccgtt
     8761 ttctgtcatc ttcgcgacat tagctgtaac atcaaattga agcgggatat cgtccacgtt
     8821 gaattcgcct cgcgtaataa tagcaataat atctgtcaat agagtatttg tatcccacga
     8881 ccccatccca aaacgcaagg tatggcttcc tgctgataat aaacttccgc tagtaacctt
     8941 gatgggggca ccggcaatat acaagtaggc gttgttccag tcaattgctt ttaatacatc
     9001 actgcttgat acatgttccg gcaaattgat gatcgccaca aatgcaaatc cttgtgcagt
     9061 gatattcacc aaatcaagta catttgttag tttcacatgg ctttcccaat gaaaacggac
     9121 cgtgccacct ttccaaggat catttgaacc atcgttcaaa taagtagcag tcaatttagg
     9181 caattggtta gccccagtta gttggacaag cttcccgcca ttgactgcac tacgtccacc
     9241 atttggaggt acgatatttt catacgtgta tggatcataa gaaatggcag gaggcgttgc
     9301 tccaacagct tctgccgaag gtgaaaagat aaatatactt aaaacaaaaa gcgaagtaaa
     9361 aaagaaggag cgaaaaaatg aaaatttatt ttgtagcaac atgttcatcc tccttttcct
     9421 ttcttctcca taacaacaac cacgcaagaa gccataattc tataccaatg atcaaataaa
     9481 gaaaacggat cttttgttca ttcgtctccg ggaatacgcc atgttgatta gattctttat
     9541 aaatcggtag ctgtgtctgc tgttttgatg cgccattgac tgaaccactt ggatgcggaa
     9601 ttggacttag atcataaaat gtaatcgtac ctgaactttt ggtatctgct tggactggct
     9661 cttgccagct gaagaacagc aaacttgcaa taatccctcc caataagatc gatgttttca
     9721 acgaaatcac ctcatcatgt tgtttctcct tttttctttt tcttgtggac ccacaaaaac
     9781 agcaaaatca accataacac tagaactgca gcaaccaatc ctgcataaag ccatgggaaa
     9841 gattgttctt gtactgcaaa aacggaattt tcatttactt tttgtgcttt ttcttttttg
     9901 atcgtaaagt tttgtgtaaa tcgccattca ttactgtcgc ctgattttgc tatgatttct
     9961 gcgcggtaat cgccggcttt tgctttatca ccagctaaat caagattcaa gtgaaaattc
    10021 gaatttggtg caactaaaaa tgcattagac gtattttcaa tataaagctg attttcataa
    10081 aagatcttgc tcgtaaatat caaattgctg ataatcctag gctgaggatt ttggaaacga
    10141 agctgaacag ttggctttgc gttgacctcg tccaaatctc ctgataaaag tttcagatcc
    10201 ggttctatcg tctcttgact ttcttgtaaa acgactgcga gattataagc aatacgattt
    10261 ttgatttgtg ctccttctgt agcattttct gtttcagctt cttctttagg tgaaacatga
    10321 atacccgcta atacacgtcc cttaaagctt tttgctggga ttttcacttc tataggtaca
    10381 gttgtttctg catgtgctga tacattgatt tcctttttcg caatgagcaa aacgtcacgg
    10441 acatcaaacg gcaatgtctc atctaacgtt gggttttttt gactataatc gatcgttcca
    10501 ccatcactcg ttaccgccgg attgacagag acctcaaatg ttcgttcttc atctgaagaa
    10561 ttgctgataa ttacttctag tgtctgattt tgttccggtg ccactaataa atcaaaataa
    10621 ccgattgctt tatctatttg gttctctgga aataccggtt tgattccaaa gtctcctgct
    10681 gaatcctttg cttggctgat tgacggaaaa atgaaaaata gacttaaaat agaaaaaaac
    10741 gaaacaaaca gtcgtttact ttgattcatt gtccaccaca tcctttcaac aacaactgcg
    10801 gtaaaaaatc cttcacctat agtattttca ccgcagtttg ttgaatttat ttcatactct
    10861 atgctactgg gccagccacg aggttccaag tcaaatcagc cgtgaatgtt ccagctgctg
    10921 ctttcggcac tttcagtgtg atatttg
//
LOCUS       DACCSK010000090        10398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCSK010000090 DACCSK010000000
VERSION     DACCSK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.092"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(126..878)
                     /gene="xth"
                     /locus_tag="IVP39_002078"
     CDS             complement(126..878)
                     /gene="xth"
                     /locus_tag="IVP39_002078"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7587142.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            991..1911
                     /locus_tag="IVP39_002079"
     CDS             991..1911
                     /locus_tag="IVP39_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7587143.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1984..2919
                     /gene="murB"
                     /locus_tag="IVP39_002080"
     CDS             1984..2919
                     /gene="murB"
                     /locus_tag="IVP39_002080"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7587144.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3217..4695
                     /locus_tag="IVP39_002081"
     CDS             3217..4695
                     /locus_tag="IVP39_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7587145.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4972..7032
                     /locus_tag="IVP39_002082"
     CDS             4972..7032
                     /locus_tag="IVP39_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587146.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7115..7684
                     /locus_tag="IVP39_002083"
     CDS             7115..7684
                     /locus_tag="IVP39_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7587147.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7859..8665)
                     /locus_tag="IVP39_002084"
     CDS             complement(7859..8665)
                     /locus_tag="IVP39_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587148.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8803..10317
                     /locus_tag="IVP39_002085"
     CDS             8803..10317
                     /locus_tag="IVP39_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7587149.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3689 a         1735 c         2047 g         2927 t
ORIGIN      
        1 ctctcgataa acagtgttca aaagccagtt tctcggtatt atgccaaatt tatgcaaaat
       61 gtggaaagcc attttcacaa aatttttctt aatactcgaa actaaacgac tttttcacaa
      121 cctctttatt tcaaatctaa ttctactgga caatgatcac tgcccataat gtccgtatgg
      181 atctttgcat cagctagtcc tggctctaaa tctttgctcg tcaaaaagta atcgatccgc
      241 caaccagcat tgtttttcct tgaattgaat cggtagttcc accaagagta gacaccttcc
      301 tgggttggat aaaaataacg gaatgtatcg ataaacccgt tatccaacag tcgagaaaga
      361 gccgctcttt cttctggagt gaatccagca tttttttgat tcgttttcca gtttttcaaa
      421 tcaatctttt gatgagcgac atttagatcc ccacacacga tgactggttt ttgttttttt
      481 agattttcca aataattata aaaggcttct tcccattcta atcggtaatc taaacgtttt
      541 aattcgcttt gagaatttgg cgtgtaacag gttacaagaa agaaatcgga atattccaaa
      601 gtaatcagac ggccttctgt atcatggata tcgataccca ttccgtaagt ggcattcaat
      661 gctggttttt tagcaaaaat cgctgtgccg gaatagcctt ttcgttccgc gtaattccaa
      721 tattgataat aacctggaag atctaaatca atttgtcctg cttggagttt cgtttcctgc
      781 agacagaaaa aatcggcgtc taactcatga aaaacttcta aaaaattttt atttacgatg
      841 gcacgaagcc cgtttacgtt ccaagagata aatttcaata aaagcacact ccttcgaatt
      901 aatttcctta cctttgcgat actattgtac ctcattttag taaaaaatgg tgtaagatga
      961 aagtaactaa atgatccagg aggaaagtag atgaagattg gtgtaatcgg tgtagggaat
     1021 atcgcagaaa aagcatattt accaacttat gcgaaaaaac aaggaacact tgatttttat
     1081 tttgcaacaa gaaataaaga aacaaaagaa agaattcaaa aagagtacgg gttccaacat
     1141 ctatatgaaa cgatcgatga gcttttagaa gaaaaaatcg aagcatgtat gatccacgca
     1201 gcaacaaaag tacattatga attagccaaa aaatgtctag aaaatggtat ccatgtttat
     1261 attgacaaac cactgagtgt ccatctaaat gaagttcgtg aattgcaaga attagctgat
     1321 aagaaccaaa taattttgat gatcggtttc aaccggcgct ttgctcctat ggtagaagaa
     1381 ttgaaacgta tcccagataa gcggttgatc cacctgcaaa aaaatcgaat agcggcaaaa
     1441 gaaacgacag agttcgtcct ttatgatttg tttttacatc ttattgatac agctgtgtat
     1501 ttattagatg aacccatttt gagaacaaaa taccagataa gagaaacaaa ggaatttttg
     1561 gaatatgcca ttttacaatt agaaacacaa aatcaaacgg ctattttaac gatggattta
     1621 gcaagtggcg ctaatataga aaaatatcaa gtaacaagta aaaatggtac ttatgaagta
     1681 aacgatctga cccagcttgt cattcaagat gctgatggaa aaaaagtgaa agaatttggc
     1741 gactggacaa acacgctagt caaacgcggg tttgagccaa tggtagaggc cttcttttct
     1801 cagatccgtt taggtaaatc ggaaacagaa ttgcttaaac aaaaagaagt tgtaagaagt
     1861 catgaattat gtgaggaagt gcttaaagat cactaccgtc atcaactttg aacaagactg
     1921 gcaagattgt attaagcgtg atagaataat gaggacgttt gagaaaagaa aggaaaatcc
     1981 tcagtgaata aagaagaaat agttgaaagc ttagccacga ttactatatt gtttgatgaa
     2041 ccattgatga actatacgtt tacgaaaaca ggtggaccag ctgatgtcct tgcattcccg
     2101 aaaaaacaag aagaagtaaa gcaaatcatc gattattgcc gaatccatga tataccttgg
     2161 atggtattag gaaatgcaag caatctgatc gttcaagatg gcgggattcg cggtgtagtc
     2221 atcatgctga ctgaaatgaa acagattcat gtcaaaggaa cgatggtcat tgcggaagca
     2281 ggtgcctcac tgattgatac gacttatgca gctttggcag aatcattgac tggatttgaa
     2341 tttgcttgcg gcatcccggg aagtgtcggt ggagcggttt atatgaatgc aggtgcgtat
     2401 ggcggagaga tcaaagatgt ttttgctgaa gtggatcttt tgcttgaaga tggaacattg
     2461 aaaactttga caaaagacga aatggtattt tcttatcgtc acagtaaagt ccaagaatta
     2521 cgtgctatcg tattggaagc tcgattcagt ttgcaaacag gggattatga agcaatcaaa
     2581 gcaagaatgg atgagttgac cgaattgcgt caatcaaaac agccattaga atacccttca
     2641 tgcggaagtg tctttaagcg cccagtagga cactatacag gtcagctgat ccaacaagca
     2701 ggattgcaag gcctgaaatg gggcggtgca caagtctctg aaaaacatgc tggattcatc
     2761 gtcaatatcg atcatgcgac agcgacagat tatattgaat tgattgcgca catccaagaa
     2821 gtaatcaaag aaaaatttga tgtgtcttta gaaacagaag tgcggatcat tggagaaaaa
     2881 caagcaatat cagaaacaag cagaagttta gccaattaat aagtaatgga acttaaaata
     2941 agtaaaagag caggacggtg gccgtcctgc tcttttttgt ggttgtttac gcaaatggcg
     3001 caaatcttgt acattttaac acaataaaac aaaaaatata gaatatttcc tcataaattt
     3061 ttccattttt tgcaatattc tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa
     3121 caagcataat ccatcataaa atcagtatag gtgaaaagat gaactttcat ctgcatatca
     3181 agtaattggt atgaggcatt ttttgaggtg taggatatga agttggatta ccttatagaa
     3241 gaggaagata gagaaaaact gctgctagct agatatttag aatttgagaa aaagaattat
     3301 gtagtgctgc ctaagttata tgaaacatta ggtttgtcta aatttaaaac aagaaattat
     3361 atcatagaat taaataaaga atttactgaa ttcaaaacaa agccagagat ccaaatttca
     3421 gaagaagcag agatccatgt cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga
     3481 atctactttc aacgatcaaa aattgctcga ctgttaacgg agatcgtgga ttcaaaagta
     3541 acgatcgaaa aatttgcaga gaatcatttt ttaagcatat caagagccta tgtaaaaaga
     3601 agagaattag tctcttttct tgaacagcat caaataaagt taaagaaaaa catattagtt
     3661 ggggaagaaa taaacatacg gaacgtatta tttggggtat atttcggggt ctttaatggt
     3721 ttttctatgc catttagaga aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt
     3781 gaatatctgt tcaatttaaa actgtctgca acaaaacgaa tcaaattcaa tttgctaacc
     3841 actattagtt tgttgagggc ctatgggaat caaccaatcg gaaaacgttt cttttcagat
     3901 gaatacttta agagctcaga aggaatgaag atcatcaagg atttatcaca tatttctttt
     3961 cttcatatcc ctaaagagca gatcattgat gagatttctt atatcatgtt gtttttgatc
     4021 acggaagaag aatttgaatt agatggcgca tttggacttg cggaagctca ttttcatgat
     4081 atccacatga ttagtcaaaa aatgtcagtg gaattgattg atcaattacc attttcgatt
     4141 gaaatctctg aagaagaaag actatatata cgaaaagaac ttcgtatggg tatgatgcat
     4201 gtcaatcgaa aaaattggat attcgacttt gagatgacta gctttacgtc taaaaagcag
     4261 ctacagtttt tcttcgagac gtatccaata ttcagtacag caatccaaaa ggtcatacaa
     4321 gagaataaac gctatttctt gggaaaagat gaacgtttca tgattcgctt attttatgat
     4381 tatctctttt tattggtcgg tatttgtccg gtatcatatt ttgaagcacc gattcatgtc
     4441 tgtattgatt tttcgcaagg atctgcttat accgattaca ttatttctca agtgaaagga
     4501 tttaaaaatt acaatattgt gattgagagt cgaatcactt ctcgtacgaa tatttattta
     4561 tctgattgtt tacttgaaaa atttaataaa gaacagataa tttggaaaaa tccaccgact
     4621 ccagatgact gggaatattt tggaaatacc atcatcagag aaaaaaatag acatttaact
     4681 gaaaaaatca gctagtattt atggatggca gctttgtctc acatatttat tagctgattt
     4741 atgttttttt caaagaaaag gtaactagat ataactagat ataactagat atattactta
     4801 acaaaattaa aaagtacata agttattatt ccgattttct tgaaacaata aaaaatcaaa
     4861 aaaaatactc tcctaatagt ccatcttctt ttgattttat ttatataatg aaatcatctt
     4921 atgaaaatta ctacttcatg tttagacaag agtagggagg actaagaata aatgtgttca
     4981 aaaagacgtt caaaacaact aaataaacac gcttgtcgtt tagataagga acggtcgtgt
     5041 tctgaaaaac actacgttaa gtttatgatg cgcacgatgc tgatcagttt atctgtaggt
     5101 gtctatatgc atgtacccgt tcaggcaacg acacaaacca tcaaggtcgt acgtacctct
     5161 gtttctgaga ccactgattc ccctatcact attactcata ctttcactga tgcaaatgga
     5221 aaacagctac ttcctgcatt gacttcctat aataaagtca gttatcctcc aacgataaac
     5281 cactactggt taattcctaa caaaacatta tggacatcgg aaaccggaag cagtgtgccg
     5341 ctttctatct tttccgatat gactgatcct acaaaaggtg atcagttaaa acagatcact
     5401 gatattttga atcatgcttt gacagatata aaggacggtg gaagcgcaca agtttcttat
     5461 gtatatgaaa aagattattc acagtttgaa agaaagaaaa tcgatgttct ggcagggaca
     5521 aaagtttcga tatctgatct ggtagaaagg ctgaagaacg cagatggaac acaaggagat
     5581 atgtccaaag taatttcaaa caatgggaat atcgtggata cgactaatac aggaacatct
     5641 actatttcct tgtcctattt tgatccaatt gctttaaaaa caatgactgc atctgctgtg
     5701 atccatgttc gtgctgatca aacagctatc aaaggaaaag atcttcactt aagaacagga
     5761 gagagttacg cccctaaaga tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga
     5821 aataaagaag aaatcaaaat gaacgggaca gtagatacta caactccggg agaatatccc
     5881 gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt
     5941 tatgtagaag catctgctgc ttcatcgact gtcatcactc attttataga tagtcaaaca
     6001 aataaagaac ttcaagcaga aacacatatg agtagtgtaa atcgtattcc tgatttgcgc
     6061 ctacctgctg aattttctga tcgagtgcca aatatagatt tatcgactta tgaacaaaat
     6121 gggaaagtca cttctatttc tgattggatg caagcaaacg gattagacag aaaaagtcac
     6181 tggtcggaga tccttgaagt acttcactca gaactgatga ataataggaa gcaatcagga
     6241 ggggaaatta cctttactta tgtttatata ccagacaatc gttcattaga aggtcatgat
     6301 cttattgttc atccaaacga aaaaataaac aaagaagagt tgatccaatc agcaacaaat
     6361 tccttaggta aacccgtacc gttaaaggat gtatcggtta caatcaatga agtaccattg
     6421 gaggatggtt atttgaccaa acaaacaggt gattttacga ttatgtatag ttatcatgac
     6481 ccgtattcac tccaagaact ccatgctcag tcactattac atgttttacc attaaacaaa
     6541 cagatagatc aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa
     6601 atggctcatg cagtttctcc aacagggaaa gtaaaacaaa caagaagcct agagttagca
     6661 agaatatcta gaaaaatctc agaatctaat aaaacgataa ggcaagaagc tcatttggcc
     6721 ccacaagttt ctggattgga aaatgaaatt ttagagccta aaataaaaca acaatcagaa
     6781 aaagaatttg ttgctaaaca gtcggaagat cagaaggaat cagatgacca gaaggaaacg
     6841 aagaaaaaac gcttgtcaac tattgattcc accgatgagc atctgcagcc gcaagtacca
     6901 cttcctagtc cgggaacacc cgcacctggt ggaggtattc cttcttctgc tttaggagat
     6961 ttcatgcgag gtatggcagg aacatgggta tacgctaatc gaaatgaatt tgacgcagat
     7021 gactggttct gatttatatc tctatctgtt atagtcaaat aagtagctga gtcatatgtt
     7081 atttggcata atggagacga ggagggatag aaaaatgtta gataaaacgt taccttacgc
     7141 agaaatctgg atgacaagac cacttcaaca aaatgttcca atcacgcctt tagcggaagg
     7201 ttttgaattt cgattttatc aaaaagaaga tgaatctgct tgggcggaga ttgagacttc
     7261 cgttggggaa tttcaaacga cagatgaagc acttgagtat tttcaacaaa actttgctcc
     7321 ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt
     7381 agcaacctgt accgcttggc gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg
     7441 gttagcagtg aaaccagatt accaaggaaa agggctcgca aaagcattga ctgccagagt
     7501 cttgcaagaa tttcctaccc tccatacgga cggaccaatc tatcttcaca cacagacatg
     7561 gagccatcag gcgattgctc tttataaaaa aatgggtttt acttttattg ctgaaaatct
     7621 agacggcaca cctaatccag attatgaaaa agttatgcag gttttaagta aattagataa
     7681 ataacgaaat ggtgaaataa gtcctcagta ttgggataaa aaacgaaata gtcattcctc
     7741 atatcttact tttttgagtt aatactatcg gataactaat ggcatgccat acattcaaaa
     7801 aagaaggggc agtgacaaaa gtattgtcac tgccccttct cttgtttttt agcatgtttt
     7861 atcgtttgat ttttttagga ataaaattct tgatgaacgt aaatttttgg taatcgtttc
     7921 gactatagag gtaaccaaat acactgaaaa caactgctcc aataaaatta acaaataaat
     7981 ccttcatagt atccatgatt cccacgtcta agtaaccatt ttggatgatt gttttatctg
     8041 ttttaccatc tgcattttga ctttcaatga ttgtccgagt gatatctgga acatgataga
     8101 cagtgtttcc gtctttactt aactccacac tattgatttg ctggatgatc gtatcttttt
     8161 gcatatcgaa ttgcagtaat ttatctgctg aaaattccac aaactcccac ataacaccaa
     8221 ctgtcatcga aaaacagaag gaaacaactg ctagtaataa aggagtcaag ttcatggttt
     8281 ttgtattttt attgagcaaa taaacaaggg aaaagccgac acccccagct aaaaatccgc
     8341 tgaatgcatg tagaaaagta tcccacattg aaaaatagcc ataaaaatca cttaactctc
     8401 ctaaaactgt tgaactaaaa ataaaagata acaaaatgat ttctagcata tttggaatct
     8461 gtatctttaa tatcttttcc agaaaataag ggatttcaaa taaaattaat gtcagtaata
     8521 aaagcattgt atttccccag tgttggttcc agacctgatc cactagcata ataacgacga
     8581 tgatacgtaa taaaatatag attccgtttg tgattctacc tgcagttttt gtactgttcg
     8641 taaaaaaggt tttcaaatga ttcataaaat aactccttca tgtatatcct taattatact
     8701 aagaagcgtg tgtttaccaa agctatatga cttaaagaaa aacagagttg atattgtgaa
     8761 aaaaatcact taatatagta taaggagaaa agggggatac aaatgaaaaa attagttgct
     8821 ggtttattaa cagccgttgc acttcttgct atgagcgcat gcggcgcaga tcagaaaaat
     8881 acagcgcaga caagttctag tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa
     8941 caagtatata cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat tattggagct
     9001 ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt
     9061 ttggaaaaga tgcctgttgc cggaggaaac acatctaaat catcaagcgg tatgaatgct
     9121 tcagaaacaa aatttcaaaa agaagaagga atcaaagact cgaatgatgc cttctatgaa
     9181 gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa tgcttcgata ctttgtcgat
     9241 cactctgctg atgcggtcga ttggctagat caaaacggta tcaaactgga caacttaacg
     9301 atcactggag ggatgagtga aaagaggaca catcgtccgt ctgatggctc ggctattggt
     9361 ggatacttag tggaggggtt attgaataac gtccatgagc gggaaatccc tatttttgtc
     9421 aacgcagatg tgacagacat taagaaaaat ggggaaacgg tcaatgaagt gactgtacat
     9481 gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt
     9541 tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg atttagctga ttatattacg
     9601 acaaatcaag aaggatcaac tggagatggt atcaccatga ttgaaaaatt aggtggacaa
     9661 accgttgata tggataaaat acaaattcat ccaactgtgc agcaggatga gggggtcttg
     9721 attggtgagg ttgttcgcgg agaaggagca atcttggtcg atcaagaggg gaaaagattt
     9781 gtcaatgaaa tggatacaag ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa
     9841 tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat
     9901 gaccaaaaag gatatgtcac aactggaaag acaatggaag aattagcaga aaaaatcgat
     9961 ttaccaaaag aaacactagc aaaaacagtt gatggatgga atacggatgt tgcggagaaa
    10021 aaagatgctc aattcaaccg tgccacagcc atggaacatg acttatcgtc tccgaattat
    10081 tatgcgatca aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa aatcaatacg
    10141 aacactgaag tattagataa agaaaaccag ccaattaggg ggttatatgc tgctggtgaa
    10201 gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa attcagttgc ggagatcatt
    10261 atttttgggc gacaagcggg catacaagca gctcgatttg cttcggaaaa tgaatgaaat
    10321 agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga gctaataccg agggtcagct
    10381 gattgaacac tgtttagt
//
LOCUS       DACCSK010000091        10245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCSK010000091 DACCSK010000000
VERSION     DACCSK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.093"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            110..592
                     /locus_tag="IVP39_002086"
     CDS             110..592
                     /locus_tag="IVP39_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587150.1"
                     /translation="MRRKTVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            789..1679
                     /gene="pstS"
                     /locus_tag="IVP39_002087"
     CDS             789..1679
                     /gene="pstS"
                     /locus_tag="IVP39_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7587151.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSTTQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2004..4475
                     /locus_tag="IVP39_002088"
     CDS             2004..4475
                     /locus_tag="IVP39_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7587152.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLETCIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4578..6314)
                     /locus_tag="IVP39_002089"
     CDS             complement(4578..6314)
                     /locus_tag="IVP39_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7587153.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKVQLLIWQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6311..7015)
                     /locus_tag="IVP39_002090"
     CDS             complement(6311..7015)
                     /locus_tag="IVP39_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7587154.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7146..7649
                     /locus_tag="IVP39_002091"
     CDS             7146..7649
                     /locus_tag="IVP39_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7587155.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7609..7947
                     /locus_tag="IVP39_002092"
     CDS             7609..7947
                     /locus_tag="IVP39_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7587156.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7968..9386
                     /gene="ffh"
                     /locus_tag="IVP39_002093"
     CDS             7968..9386
                     /gene="ffh"
                     /locus_tag="IVP39_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7587157.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKNFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9498..10076
                     /locus_tag="IVP39_002094"
     CDS             9498..10076
                     /locus_tag="IVP39_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7587158.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10080..>10245
                     /locus_tag="IVP39_002095"
     CDS             10080..>10245
                     /locus_tag="IVP39_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HAP7587159.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTSIMGRKTYQ"
BASE COUNT         3340 a         1857 c         2150 g         2898 t
ORIGIN      
        1 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
       61 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
      121 aacagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
      181 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
      241 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
      301 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
      361 cttattagta tttacatcta tgttttcttt gctgcctcgc ttgattatcg gccctaatgg
      421 tttgagcgag agtggattga aggattcagc ttattcctcc atgtatttct tgacgatcaa
      481 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
      541 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
      601 catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
      661 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
      721 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
      781 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
      841 gcggctcagg atcaggaaat acagatggct ctaccacaca aaataattca agtgactcaa
      901 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
      961 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1021 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     1081 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     1141 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     1201 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     1261 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     1321 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     1381 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     1441 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     1501 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     1561 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     1621 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     1681 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     1741 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     1801 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     1861 aactgtcgta gctttttttt gctatatgac aggaatgaaa ggtgtttgca agcttaatgt
     1921 gaggaacact ttccatatct gttctatact ggataggaga agtttggaaa tatccagaaa
     1981 acgaaatcaa tcgaggtagc cacatgaaga ataaatggat aaatcgaagt aaaaaaaaga
     2041 aagtaacaca aagatcatca gataaagtca tccgtctttt tccatcgtat aaagaaggat
     2101 taacggcaga tcaagtcaag gaacgaatag aaaaaggtgc agcaaataat tcagtcgatc
     2161 ccacattcaa aacaaatcag caaatcgtat tggaaaacat atttacgtat ttcaatttga
     2221 tctttcttat cttagccatt ttgctttgtt tagtagaatc ctataagaac ttgacttttt
     2281 taccagttat catagccaat acaggaatcg ccatttttca ggaaattcga tccaaaaaga
     2341 tcttggacga actaaatgtt ttaaatacag caaaagtcaa tgtcatacga gacgggatag
     2401 aacaaacaat agatatagaa gaccttgtaa tagatgacat tgttatttta gagactggac
     2461 atcaaattcc agcagatgca gaggtcgtgg aagggaaact gcaagttaat gaggctttat
     2521 tgacaggcga agcagatgaa ataaccaaag aaataggcga tccattaatg tccggcagtt
     2581 tcattgtttc cggaaaagct tacgcccgat tagacaaagt tggagcagat tcgtatattt
     2641 cccagctgac agtcaaagcc aagacaatgg gagaaggaga acagtcggag atgattgctt
     2701 ctcttaatca attgatcaaa tggattggga tcatcattat tcctatcggt atcctgcttt
     2761 tttctcaaag ttactttttt aatggaaaca cgatcaaaga aagtgtcgtt gcaatggaag
     2821 cagcattgat cggtatgata cctgaaggac tgtacttgct gacgacgatt gcattagcat
     2881 taagtgcgac taaactagca aagcaaagag ttcttttaca caatatgaaa agcattgaga
     2941 cattggcaag ggtaaatgtc ctttgtgtag ataaaacagg aacgatcaca gaaaataaaa
     3001 tgtctgttca aaaagtgatc gtctctaaaa aacaagaaat aatcagtcag tcagatcaat
     3061 tggaaacatg cattgctgac tatgcaaaag cgatggcagg agacaatgca acaatggaag
     3121 ctgttaaagc atattttaca aaaaccactg atcaatccta tcaccatgtt attccttttt
     3181 cttctgtcca aaaattcagc agtgtagcgt tggcagacaa ggtgtatgtt ttaggagcgc
     3241 ccgaaatggt tttgcgcgat caattgccgg aatatgcgga agagtttgtt tctttcgctg
     3301 aaaaaggcta tcgcgtgttg gtctttggtg tgtacgatgg gattcttgaa gaaccagtat
     3361 tgacagaagc agtcacacca ttaggctata ttctgattgc taatcctatc agaaaagaag
     3421 caagagccac atttgattac ttcaaaaaac agcaagtagc catcaaagtg atttctggag
     3481 ataatccgct caccgtatcc aatgtagctg tccaagcagg gatttcacat gctgatcagt
     3541 acgttgatgc atctaaatta tcagaagaag aatatgaagc agctgtcgaa aaatatacag
     3601 tttttggacg cgtcaagcca gaacaaaaga aaatcttcgt tcaattgcta aagcgtaaca
     3661 atacagtagc tatgacaggt gatggcgtga atgatattct agccatgaaa gaagcagact
     3721 gcagtatagc gatggcttcc ggaaatgaag ccgccatgca ggcctcacaa gtggtcttac
     3781 ttgattctga cttttcacgc atgccagaag ttgttgcaga aggacggcgc gttgtgaaca
     3841 atattgagcg atcagccagc ttgtttttag tgaagaatat tttctccttt ttgctctcac
     3901 tgttttcagt gatctttgct ctgacctatc ctttagaacc atcacaaatc acattgatta
     3961 gtttgtttac tatcggactt ccatctttct tgcttgctct agaagaaaac aaaaagcgaa
     4021 tcagagggaa attcatcatg aatgtgatgg aaaaagctgt gccaggtgga ctaacagata
     4081 tgatcgtagt cggtgcgttg gtgatctgcg gggtgacttt gaatctaaat aaaacagatg
     4141 tttctacagc atctaccatg cttttgattg cagtagggtt cctcgttctc tataaaatct
     4201 gttcaccgct gaataaattc agaagtcaga tcattctttt ctgtgctagc ggtaactttt
     4261 tttcggtcat ctttttgcat aagctgtttt caatcaccgg tatctcagct gtatcacttt
     4321 tactgttgag cattctattt ttctcggcag attcgatttt taggcatttg accacattgg
     4381 tagaatattt gtttgaacgt aaaaatgaaa aagagcaaaa gccgtttagt cgcgtagtta
     4441 aaaaatttct atcgatattt aaacgagaaa attgacgaaa agtatctcaa aaaagagtaa
     4501 aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag tcatccaact ttaaaacgca
     4561 cgagcagttg ctctttttta cattttcgga atggtaacgg tgaaagtcga acctgttcca
     4621 agatgactgt ctacagtaat cgtaccatga aggagttgca cgtagtcttt tacaatcgct
     4681 aaccctaatc cggtcccgcc tgaatgtcgg cttcgcgcct tgtctacgcg gtaaaaacgc
     4741 tcaaagatcc gttcctgatc ctcaatgtcg atacctatac caaagtcttg gacagaaaac
     4801 gatagatcat ctgttgcctg atagcggata ataatctcgc tgtcgctttt tgaatattgg
     4861 acagcatttt caatcagatt tttgataatt ggataaagga tatctgtttt agtagtaaat
     4921 ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct tttcgatgat tggttgataa
     4981 ttttgaacga tttgctggaa ataaggttct gtataaattg tctggatctc gtaagaattc
     5041 tcgccatctt ttgacagctg gatgatttcc cgaatcagtt tatctaaccg gatcgcatct
     5101 ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt catcttttgc accatccagt
     5161 aaagtctcgg taaaaccaat cagtgaagtc acaggagttt ttaattcgtg agagacgttg
     5221 ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc atacgccacc
     5281 cctagaatct gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa gtctaatgtc
     5341 tgccgacctt ctgtaagggt gatctcctga tggatcagcg gatgctcagg agtcacttga
     5401 tgaatcagct gaatgagttt tggctcttga atgacttctg tgtaccgctg ttcaggttga
     5461 tagcttacca cgtttaaatg tagctgcatc tttggattaa gcagtagtaa acgagaatca
     5521 gcatcatcaa tcaggaatac gccaatcatc agttcgttca gtaacgtata taattgctct
     5581 tctgtggtag tatacgcccg atacatctta ctcatttgtt cgctcaacgc attgatcgtc
     5641 tgatacaatt cttcccattg atcgggtgtt tgcatgatta cttccgcctg attaggattt
     5701 tgcaccattt tttttagtac gggcagcacg gttttcaatg gctggttttt ttgatagatc
     5761 agataataga tcattcctgc aagaacaagg aagaaagaca gaaagaagaa aaagacccaa
     5821 cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg gttctgcgat acgtaaaatg
     5881 gcttctaact ggccactttt tttgactggt aatgcgacat ataaaagatc ctcattcaaa
     5941 gtagtacttt tgcgcaaagc gctacctagg ttcccaccat tcaatacggc tttgatttcg
     6001 ggacgggagt ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa gatctttcca
     6061 gtagcatcca ttaaagtaat ccgttcgttc gactgatgga cgaattcttc caaagcagtt
     6121 ttgttttgcg ctgcttggag gttttcgaca tctaattgcc agatcagcag ttgtactttt
     6181 tttgtcaggt agctttcttg ctgttccacg atctgctgtt gaaaataatg ggaaatcaat
     6241 tgccagccac caaaaaacaa actaactaaa agaataagaa agaagagacg ttcataaatt
     6301 tttttccatt tcatcgtttt ggttcctgaa acttatagcc aaatcctcta atagtcacca
     6361 agtactttgg atgttttgga tctcgctcga ttttttctcg caaatgacta acgtgtacat
     6421 ctacgattcg gctttgtcct gcgaagtcaa aattccaaat gcggtctagc aatgtgtctc
     6481 ggtcgatcac acggtctttt cgtttcatga aataaacaag taattcaaat tctttcggtg
     6541 ttagttcaat cagatggtcg tctacggtca cttgataatt ggtcaaatca gcagtgattt
     6601 gtccaatcga tagatattca ggctcagctt catcttttga ttgcttacga ggttcaatcc
     6661 gtctgaatat tgctttcata cgggctaata cttcgcgagg actaaatggt ttggtcaaat
     6721 agtcgtcagc acctatttct aggccaatga tccgatccac ttggtcatct ttagccgtca
     6781 gcatcaaaat cggtgtttca tttttttctt gacgcagccg ctgagtgatc tccatgccat
     6841 ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa tgattgttct aatgctaatt
     6901 ctaatccttc gcctccatct gtggcactgg ttaccttgta gccttctttt tccaaattga
     6961 acgtcaagag cgttacaata gaaggttcat catcaacaac tagtattttt ttcattcaaa
     7021 tctcctttaa ataggtttca ttctgtttat attttagcat agaatcagta caaagggcta
     7081 ttttacaaaa gaaataaatg gtataatagg aaggcacttt gctgaataga aaggaagagc
     7141 agtcaatgat tggaatcagt gcatgtttag gcggtctggc ctgtcgctat gatggacaga
     7201 aaaaagaagt accagaattg atggaactag ttgaaactgg agaagctgtc atggtctgtc
     7261 cagaagtgat cggcggatta ccaattccta gagatcccgc tgaaatcatt ggtggaaatg
     7321 gattcgatgt gtggaaagat caagcaaaag tatggacgac aagcggcgaa gatgtaacgg
     7381 aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa cacattgatc
     7441 tagtcatcat gaaggaaaaa agcccttcat gcggaaccca ttcgatttat gacgggagtt
     7501 tttccggaat aaaaaaaaat ggtgccggag tggctactgc ttattttatt cagcaaaaaa
     7561 tgactgtgct ctctgatgaa gaatggctga aaactagagg tgagcagaat ggaaatcgaa
     7621 aagaccaatc gaatgaacgc gctctttgaa ttttattcga ccttattgac agaaaaacaa
     7681 atgaactaca tggaattata ttatgcagat gatttttcat taggtgagat cgcggaagaa
     7741 tacgaagtaa gccgacaagc agtatatgac aatatcaagc ggacaagcaa gatcttggag
     7801 gattatgaaa aaaaactcca tcttttttct aattatattg ttcgagaaca ggtattggaa
     7861 aatatgactg catatattac agataagtac ccggaagata aaaaactact tgaatatgta
     7921 cagcaaattc aagcaatcga agaataaata aaaggaatga agaaaatatg gcatttgaaa
     7981 gcttaactga acgtctccag caggcgatgg gaaaaataag aaaaaaagga aaagtatcag
     8041 aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc cttactagaa gccgatgtta
     8101 acttgcaagt agtcaaaaat tttactaagc gcgtacgcga acgtgcggta ggggcagaag
     8161 tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa gaattgacga
     8221 tcacacttgg ttctgaaaca gcagagttaa ataaatcacc aaaaatacca actgttatca
     8281 tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta gccaattatt
     8341 taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat cgaccagctg
     8401 cgatcgacca attgaaagta ttaggtcaac agctagacgt tcctgtattt gatatgggaa
     8461 ctgaagtcag cccggtagag atcgtccgtc aagggatgga gttagcaaaa gaaaagaaaa
     8521 acgactatgt attgatcgat acggcaggac gtctgcatat cgatgaaaca ttgatggatg
     8581 agttgaaaca aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt gtcgatgcca
     8641 tgactggtca agatgccgtc aacgtagctg atagcttcaa ccagcaatta ggtattacag
     8701 gggtcgtcat cactaaactt gatggggata ctcgaggcgg ggcagcacta tcgatccgct
     8761 cagtgacagg cgcaccaatc aaattcatcg gttctggtga aaaattgact gacttagaag
     8821 tattccaccc agatcgtatg gctagccgta tccttggtat gggggatatg ctgacattga
     8881 ttgaaaaagc gcagcaagac tatgacgaga aaaaagcgga agaacttgca cagaaaatgc
     8941 gggaaaattc attcgatttc aatgacttta ttgaacagct tgatcaagtg atgggtatgg
     9001 gcccactgga agatctaatc aaaatgatcc caggaatgaa tcaagtccct ggaatcgaaa
     9061 acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc gatggtttat tctatgacgc
     9121 cagcagaaag agcaaatcct gatctgttga acccaagccg tcgtcgccga attgctgccg
     9181 gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag gaatcaagaa
     9241 aaatgatgca gcaaatgtcc aaaggggata tgaacatccc aggtatggac caaatgtttg
     9301 gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc aagaaaaata
     9361 agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca gactgaaaat
     9421 gtctactgcc ggagcttttc tatcagcaat ttttattctt tactaataca attactctaa
     9481 acgaagaggt ggcacagatg agatgtccgt ggagtgaaca aacagaaagt atgcggcaat
     9541 atcatgatac tgtctggggt gttcctgaat atgatgacca aaagctgttc cgaaaattga
     9601 tgcttgatat taatcaagcc ggattgagct ggcagacgat tttaaataaa agtgaagcat
     9661 tcgatgaagc atatgatcga ttcgatatca aaaaagtcgc aaattatgga gaaacaaaaa
     9721 tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc gaagcagccg
     9781 tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat cggcagtttt tcagagtatc
     9841 tttggtcgtt ttctaatgga aaaatcgttg attcttctta tcattcacaa gaagaagtcc
     9901 caacaacaag tcctctttca gatgaattag caaaagatct aaagaaaaga ggatttaaat
     9961 ttatcggaag cacaacaatc tatgcttttt tagaagctgt cgggatcatc aatgatcacc
    10021 ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc gaataaagca
    10081 tgcgaaaaat catattttat ggtgctataa gtttagatgg ctatttggca acgaaaacgg
    10141 atgatcttca atggcttttt gacactccaa caggtaagca aaccacttat gaagcttttt
    10201 atgaaacaat cgatacgtcc atcatgggca gaaaaactta tcagg
//
LOCUS       DACCSK010000092        10155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCSK010000092 DACCSK010000000
VERSION     DACCSK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.094"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(311..2203)
                     /locus_tag="IVP39_002096"
     CDS             complement(311..2203)
                     /locus_tag="IVP39_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587160.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2200..3927)
                     /locus_tag="IVP39_002097"
     CDS             complement(2200..3927)
                     /locus_tag="IVP39_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587161.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3943..4593)
                     /locus_tag="IVP39_002098"
     CDS             complement(3943..4593)
                     /locus_tag="IVP39_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7587162.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4797..5093
                     /locus_tag="IVP39_002099"
     CDS             4797..5093
                     /locus_tag="IVP39_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7587163.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5285..5905
                     /locus_tag="IVP39_002100"
     CDS             5285..5905
                     /locus_tag="IVP39_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7587164.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5997..7163)
                     /locus_tag="IVP39_002101"
     CDS             complement(5997..7163)
                     /locus_tag="IVP39_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7587165.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7175..7528)
                     /locus_tag="IVP39_002102"
     CDS             complement(7175..7528)
                     /locus_tag="IVP39_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7587166.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7656..8678
                     /locus_tag="IVP39_002103"
     CDS             7656..8678
                     /locus_tag="IVP39_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7587167.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9117..9551)
                     /locus_tag="IVP39_002104"
     CDS             complement(9117..9551)
                     /locus_tag="IVP39_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587168.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9661..>10155)
                     /locus_tag="IVP39_002105"
     CDS             complement(9661..>10155)
                     /locus_tag="IVP39_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP7587169.1"
                     /translation="LENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSD
                     TIQAKNDKQTLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQL
                     LLSLEQELPAFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGF
                     FEHE"
BASE COUNT         3028 a         2058 c         1845 g         3224 t
ORIGIN      
        1 agaggttgtg agcctgccgt ttaactccga gtcacaagga gcaatttcaa aaaacagctt
       61 ctcaggtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa
      121 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agatacaaaa cggaaaaata
      181 gcttctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccgtt
      241 tattcggaaa aagggactgt gacacgactt ttgtcacagc ccctaaaaaa aataataaat
      301 ttgtactatt ctatgaagct tcttcttcag aaaactgact gttataaaga tctgcataga
      361 atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca
      421 tcacaataat gttgtctgca tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg
      481 tccggttttc caataggcgg ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta
      541 cactagatgt tgcttcatca aggatcagga catctggatt tgctaagaat gcccgcgcaa
      601 ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt
      661 tatatccttc tggcaattta cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa
      721 tgatttgttc gtctgttgca tcgtcgttcc catatttgat attgtcatag atacttcctg
      781 taaacagcca agtatcttgc agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa
      841 gatcacgggt atctacacca tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt
      901 caagaaggtt gatcagtgtc gtcttccctg caccagtcgg acctacaata gcgaccatct
      961 caccaggttt gacatccaag ttaaaatcag tcattagcaa gttatctggt gtgtagccga
     1021 attgaacatt ttcaaaacga actttgtatg gtgtatcttt ttcttctgga aggccagatt
     1081 tttcattgga catctcttct tcatccaaca cttcaaatac ccgttcggct gaagcaactg
     1141 ttgattggat agtgttcatc aaacttgcaa gttgagtgat tggttgagag aactgtgttg
     1201 tatattgcaa gaacgcttga acatccccta atgtcatgtt cccatttgca acttttactc
     1261 cgcctagtac agcgacgaac acatagccta gatttttaac gaaattcatc aacggcatga
     1321 tgattgccga aataaattga gctttccaac cagcggcata cagattctga ttttcttttt
     1381 caaatacttc ctggtctttt tcagtatgat tgaatgtttt aacaatggta tgaccaccat
     1441 atgtttcttc tacttggtta ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca
     1501 cttgtgattt cggcgctacg atcatcacga tgatcaagct taaagggatc atcaataacg
     1561 cgactaacgt caattgccag ctgattgaaa gcatcatcac gatgacccca atcaatgtga
     1621 ctgcactagt tacaaattga gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat
     1681 cattgattgc tcgagacatg atatccccat ttgtatgtgt atcataatag gaaatcggta
     1741 cacgattcat tttggcttcc aaatcatgac gtaattcata aactgttcgt tgggaaacac
     1801 gtgtcataat aaattgctgt aaaaagttga atacagcaga aatcaaatac atcacgatta
     1861 ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg aaattttgtt acttgttggc
     1921 cttgttgcat ctgttgagcg ccagccatta cacctttgaa tatttctgtg gttgctttcc
     1981 ccaagacctt tggtgtttgg atttggaaga tcacggctgc tattgctagt acaaatacag
     2041 cgatgatcgc aaccatacgt ttagacatat agccaaacaa tcggcgtacc gttccccaga
     2101 aatttttagg cttttcacct tttacaccga gatttctgcc aggtccacca tgtcctggac
     2161 cgcctgctgg tccatgagag ggacgtgtgt ttgttttact catgctaagt cctcctctct
     2221 tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt
     2281 tcctttgcca accagttttc cttcatctag cacaaggatc atatctgcat ccattactgt
     2341 acttactcgt tgagcaacta aaacggtgat actttctttc atattcgttt tcaatgcttg
     2401 gcgaagattg gcatccgttt tgaaatccaa cgctgagaag gaatcatcaa acacatagat
     2461 atctgctgtt ttgactaatg cccgtgcaat tgccagacgc tgtctttgtc caccagagaa
     2521 gtttcctcct ccttgttcca catggctatc taagccatct tccatctctg aaacaaaatc
     2581 tttagcttgt gcgatttcta atgctttcca aatcatttca tctgttgcat ccggcttacc
     2641 atactgcata ttgtctcgga tcgttccaga gaacagaact gctttttgag gaacaaagcc
     2701 cattaattct cgcaaattat attgagtcat gtcactaatg tccgttccat ttatttggat
     2761 cgaaccagat tcgatgtcat ataatcgagg gatcaaattg actaaggtcg ttttacctga
     2821 accagtcccc cctatgattg cgacgatctc gcccgacttg gcttggaaat caatatcttc
     2881 caatgcaagt ttttctgctc catgatatcg ataattgaca tgattgaagg ccaatgttgc
     2941 gttttttcca gcaaaagaaa ctgttttagg attttcaggg tcatggatac cgattttttc
     3001 atccaacact tcattaatac ggtcagctga agcttgtgca cgaggcacca tgataaaaat
     3061 agcggaaagc atcataaagc tgatcaagat ctgcatcgca taagtcataa atgcaatcag
     3121 attaccgact tccaaagtca tatctgctat gtagtgtcca ccaaaccaag tgattgctac
     3181 gtttgtaccg ctcatgatca atgtcatcat tggcatcatc aatgcgacga tcgtattaac
     3241 tttgattgcc gtatttgtat aatctttatt cgctaaatca aaacgatttt cttcaaagcg
     3301 tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat ccttctcgaa ataccaggtt
     3361 taaacggtca gttttctttt gcatactttt gaataatgga acagcaaagt acatgattcc
     3421 gccgatcaaa ataatcatta ctgggatgac aactaagaag atttttgtca actgatgatc
     3481 tttttgataa gcaagtatgc tcgcgccaat caacgtgatt ggtgcattga tcatcatacg
     3541 taaaaacatt tgtgtaacca tttggatttg agtcacatca ttggtcgttc ttgtgatcaa
     3601 agaggccgtt ccatatttat caaacgcatc ttttgtcaga ctctctgatt ttttatagac
     3661 atccgtacgc aattgcttac ccagtttctg tgattcacga gtagcaaaaa atgtatttgc
     3721 gatagcagct aagatactga tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat
     3781 ataatcgaca tctccttgcg ctaccccttt atcaataata ttggaggtaa gtgtcggcaa
     3841 atataaatct gccagtactt ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa
     3901 ggagatccgt tttacgattt ttagcatcta gttcccttct ttctatttat atactccgtt
     3961 tttcagccaa gaaagtttca aattgaattc actgattgct tgctctactg agtaatcttc
     4021 agatgttgaa aataagcgat acgaatgcgc aattgttgta aatacagtat tcatcactaa
     4081 ttggatcagt aattccaatt catgatcatt ttttactttc agcttcgtcc gatcgatcaa
     4141 ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg
     4201 gcgactcgta tgaggagcga cttttcttga ggcacggtaa tccatgttca taaacaagtt
     4261 acgataaaaa gaagcattct cgccttccaa aatctcataa atcattttag aaaaatagga
     4321 ttcgaatcct ttgaatagat caccatcgga ttctttcatt agttgtaaca tatctttaga
     4381 gctgtcacgt ctcattgtct caaagtaata aaaataaaga tcctctttat cttcaaaata
     4441 ctgatagaaa ctgcctctag ggatatccgc taatttgacg atattagcaa tcgaagcttc
     4501 ttttaagggt actctagaaa actcgattct cgcagcctcg attagtcttt tttgtttttc
     4561 tttaggcaaa tggaaaaatg tctgtgtcgg catatggatt ctcctttctt tcaagatgac
     4621 acattgtcat atgacgctat gtcattataa cacctcctac ctgttttgca agacatttgc
     4681 atagtttttt tcattatcat ccgatacatt aaaaaagtga taagaggaat ccttccattt
     4741 gaattaatga gagttgccca ttaaaatagc cacatactaa acaaaggatg agataaatgg
     4801 cccaatcaat tagtgtccaa gacttcaata aattgcctct cgatgctgac agcatcgttt
     4861 tagatacaag aaatacctta gattacacaa aaaaccactt aagccactcg ctttctatcc
     4921 ccgctgctat cttgccaaga caattaaaaa atctagatcc aaagaaactt tattatattc
     4981 taagctatac aggcaggcga tcagaagcat tagccgatca acttactgct aatggttttc
     5041 gtgctgttta tatcattggt gggatggaag ctcttcagtc gagtgttgct tgaatattgc
     5101 ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt
     5161 ctttttttag ttatttattc ttcaacctta tcaatatgac ttgatagcct tttctttttc
     5221 gagtaaactg attttgttag tcatagttat aacaatcata gcaataaaag gagaaggtaa
     5281 gcatatggca tctgttttag tagtcaaagg tcatccccta actgcagaag aatcgcgcac
     5341 agttaaggca ttgacgtctt ttttaacaag ttataaagaa aatcatccag atgatgaagt
     5401 gactgttttg gaactttatc gcgatgatat tccggaaatc gatgaagaat tattatcagg
     5461 ttgggaagca ttacgtgctg gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa
     5521 aattgctcgt ttcaatgaat tgactgatca gttccttgct gctgataaaa tcgttatcgc
     5581 aaacgcctta tggaatctaa atatcccaac aaaattaaaa gcttggttcg ataccgtcaa
     5641 tgtagctgga aaaacgttcc gctatacaga aaacgggcca gaaggattag tgacaggtaa
     5701 aaaagcactg cacatccaat ctaatggcgg tgtatacaac ggacaagact ttgcctctca
     5761 atatgtaaaa ggtattttaa actttgtagg catcgatcaa gtagatcagc tattcatcga
     5821 aggaatcgat tatgaccctg accgtgcaga tgagttgatg caaaatgcat tagataaagc
     5881 tgctgcttta ggtaaatcat tttaaaaatt gagataaatg catacaaaag agacagagga
     5941 aaaaatcttc aaagcagcat ttgtgccttg gatattttcc catctgtctc tttttgttat
     6001 tctttctgct ttttcaacgg attttcttca taaagagaag agattgttgt gtaccattca
     6061 aaaacatgag taatcactac tttagctgct gcataaacag gaatccccaa gatcacaccg
     6121 atgattccga acaatttccc agaagtcagc agaactaata aaatcgttac tggatggatt
     6181 gctaactgat tccctagtac taatggagag atcaagcgtc cttctattgt ctgctctact
     6241 gcaaaaacga tcagtacttt gatgatcatg aatggacctg ctacaatggc taaaaagata
     6301 gccggaatca tcgctaaaaa tgatcctaag taagggatca ggtttaaaaa acccgcaata
     6361 atccccaacg tgacagcata ctctaaacca atgaccgcaa aaccaatcat aaacatgatt
     6421 gccacagcaa aagccacagt tagttgtcct cttatataag aagaaacttg actattcatt
     6481 tcactcagca cattcaacgt cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa
     6541 ggtgctaatt gtttaccgtc tttcaataga taaaataaga taaacggcat cgtcagcaga
     6601 gccactaaaa tagtcgctac cgcgccgaca aaacttccaa gactctgaac tgtactctta
     6661 gatatatctt ggatcatatc gcctgccgaa ttcatgaact tatcacctgc tgtttccaac
     6721 tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct cggttaattt attatcaatc
     6781 gtatctacat attgagggaa gttactgatg aagctcactg tttgttcctg aatcttaggg
     6841 atgatcacta cgctccccca tgcgattagt gcaacaacca aaacaaacag cccaatgata
     6901 ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa ctaccggatt catcaagtaa
     6961 tacaagattc ctgctaaaat gataggcaga cctacgattg ctaaaaattg ccagattggt
     7021 gcaaacaaat atgaaacttt tgtaaataaa aagacgatca gcaatattaa caatacaaca
     7081 agtaaggctg tgactacttg attatttaaa aaccagcgcc aaaaccacga taccggtctg
     7141 ttcttcttat tttttccttc cataattcct cctgttagtt ataaaaaatt tggcttccca
     7201 aaccaatagc aggcagtcta tgcggatgaa gatagattcc atttgcaatt tgtccttctt
     7261 ctggacaagc atcaaaaatc aatgatacgt gagctactgt ctttaaattc ccattggcca
     7321 tcggaccgac ataatcgatc gtatatacct gatcatcaaa agcaattgtt ccgcctgctt
     7381 tcaaagaaaa aggttcgtct tttgatactt cttgaatcac cgaatatttt cgtaaggcag
     7441 ctgttgctgt tttatcgaac aaaatcagca tgggttcttc cttgtttatt gcttctgtcc
     7501 caatttctgt aatctttgcc tgaatcattg cactctctcc tttaattgct ttcaaaaaca
     7561 gtataacaca ttctcattct ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa
     7621 aactatttca ttacaattag gaggaatcag ctcatatgat cgaaaagata attttaggaa
     7681 cctatacgcg tcgcgaaagt aaaggaatct atacgatcga ccttgataca gaaaaagaag
     7741 aattagccaa cctttcatta gctgttgaag aaaatagccc tacctatgta gcaaaaagta
     7801 aagctggcaa tctttacaca gttactagtg tggatggatt aggtggtgcc ggtgcgtacg
     7861 ataaggattt tcattttttg aatgcggtaa cagagtctgg cgctcctttg tgttatgtag
     7921 cggttgatga agcacgtcaa ttactatacg gggcgaacta tcataaaggt gaagtaaatg
     7981 tctaccatat attaggagat ggcgaactga aagcagcaga ttcaatattc catcaagaag
     8041 cgacaggtcc tcataaaaac caagatcatg cgcatgtcca ctatacggat ctgactcctg
     8101 accaacgatt agtcgtatgt gatcttggta cagatcgtgt gtatacttat gatgtgtccg
     8161 aaaacggcaa attgaatctt gtaactacct tcactgcaga gccaggaaca ggccctcgcc
     8221 atcttgtctt tcatccaaat ggacaattcg cttatttatt tggtgaatta gacagcacag
     8281 tccgcgtgct ttcttataat aaagaaacag gtgcttttga agaattacag aaaatatcga
     8341 ccttgccaga agaattcgac ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg
     8401 gcaagttcct ttatgcttct aaccgaggac ataactcgat tgctgtattc gcagtttttg
     8461 aagatggttc ccacattcaa aacaagcaaa tcatctcaac agaaggggat tttccccgcg
     8521 atttcgcact agatccaagc aataattttg tcgtgtgtgc caatcagaac accgataatc
     8581 tgacattata tcgccgtcat cctgaaacag gattactgga attactgaaa aaagatgtct
     8641 atgtaccaga atgtgtctgt gtttattttg atcgttagta agaaaaggag aatgaaatga
     8701 atcagcaaga cgtattagac agactacggg aagaactaaa tattcctttc tttgatggga
     8761 aaatcgaaga taaagagtat agcgaagagg aatatcagaa actcaaagcc gatttacaga
     8821 actactttga acaatatgta cagaatgtag aaaactaatc agctttccat actatctgaa
     8881 aagaacatca ggacaagact tttccgtcta tcctgatgtt cttttctaca aataaaaaac
     8941 cgccgagatg gttagactcg acggaaaagg agtatttact cgattttgag taagtacaag
     9001 gttgttgttg gttataggta tgtcattatc ataaaggatt ttgatcactc tatcataaag
     9061 gtaatattac atttatatta gataactcta gtttttattg ttatcaaaga tttcttttat
     9121 tttttcaaca acaaattagt cattttttgg atggcagctg tcaattggtc tgtcgcttgg
     9181 tcttgttttg cttcagatgt attgaataga tcttcatcta cccctgattc aagatcgata
     9241 ctttggatca tttcttcaca gcctgctaca tatgaaagat aagctttttc aaaattttta
     9301 tgaatgccca ttgcttttgc tggtggacgc aaagtactga ctttcataag cattgcccga
     9361 tatttttttg ttccttcttc aaataacagg atgatctctt caagagtcga tttgtccagt
     9421 tcacttgttt ttccttcatc aattgcgttt ctgatcactt catatttcgg atgcattcct
     9481 tctccgattg cttctgtttc cttgacgatt tgatcgatgg actgcacata atagccaata
     9541 ttcggtaaca tgttcgtttt cctactttct ccattgttgt tattatttat tctacttgtt
     9601 tttatcctca aatgacaagt acttgaaaaa atttaatggt ttctatacag ctcgccattc
     9661 ttattcgtgc tcaaagaaac catctgctaa actatattgt ttccctgcga caatgtcata
     9721 ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt
     9781 ataccactgt ccgttttgat agtacagttc tcgttcaaac gccggaagtt cctgttctaa
     9841 tgataaaagc agctgataaa agccatttgt tgtctgattc gtctgttcca ttagattcgc
     9901 tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt tgcttttcta attctccttt
     9961 actgtcgaac ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat
    10021 cgtatctgaa tagatccctg gaagatgatc cccccaaaaa acaactaaag tccttctaga
    10081 aagattcttc aattcttccg tgaactgctt cagtgccgtg ctgctatagg aaatatcttg
    10141 cagataattc tctaa
//
LOCUS       DACCSK010000093        10148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCSK010000093 DACCSK010000000
VERSION     DACCSK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.095"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(644..1624)
                     /locus_tag="IVP39_002106"
     CDS             complement(644..1624)
                     /locus_tag="IVP39_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7587170.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1637..2515)
                     /locus_tag="IVP39_002107"
     CDS             complement(1637..2515)
                     /locus_tag="IVP39_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7587171.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2531..3331)
                     /locus_tag="IVP39_002108"
     CDS             complement(2531..3331)
                     /locus_tag="IVP39_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP7587172.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            4723..5049
                     /locus_tag="IVP39_002109"
     CDS             4723..5049
                     /locus_tag="IVP39_002109"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7587173.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            5057..5965
                     /locus_tag="IVP39_002110"
     CDS             5057..5965
                     /locus_tag="IVP39_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7587174.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6594..6845
                     /locus_tag="IVP39_002111"
     CDS             6594..6845
                     /locus_tag="IVP39_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP7587175.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            7387..7545
                     /locus_tag="IVP39_002112"
     CDS             7387..7545
                     /locus_tag="IVP39_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587176.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7617..8843
                     /locus_tag="IVP39_002113"
     CDS             7617..8843
                     /locus_tag="IVP39_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7587177.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(8932..9324)
                     /gene="rpsI"
                     /locus_tag="IVP39_002114"
     CDS             complement(8932..9324)
                     /gene="rpsI"
                     /locus_tag="IVP39_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7587178.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(9340..9783)
                     /gene="rplM"
                     /locus_tag="IVP39_002115"
     CDS             complement(9340..9783)
                     /gene="rplM"
                     /locus_tag="IVP39_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7587179.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         3159 a         1958 c         1726 g         3305 t
ORIGIN      
        1 taaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt
       61 cttcgccaac acaatttgac gaagagcctt ttctttcagg aagacttcgg taatttttag
      121 gagttctacc tattctttta accgtttatt ctctttttct aagcgtgtaa tttcatttct
      181 gcgaatggat ttcatgactt cctctaccga gaaaagtacg ttatccatat ttttcaactt
      241 cttgaactca tcaatacacg ctattagagc gagcttctag ctatagacta acttctttaa
      301 cagaaaaccc ttcttccgtt accagtttta cagcggtaat tttaaatatt tcatcaaatg
      361 aacgtcttgg cataatccag atcttcgctt tctcttaatt tggtgtcaat tttagtataa
      421 cccgtccaac ttatttttta actaagtgcc cggtttttct tgacaactcc acccaaaagt
      481 tagatttttt ggtctaagtt ttagggtcgc ttcattacta gaattcaaca agagatgttt
      541 tttattttat ttaaattatg gggtcagttt ccatttgatt acaaacttct ttgttttctt
      601 tttttaaacg caataataaa ggttttaatt tcagcctttt taattagaaa atcaattttc
      661 ccaaattatt ttctttagct ttcttaacga tttctgtcgc aactaccaca tctaatacag
      721 ccgaaccaac tgttttgaag atcgtgattt gttctccact tgtccgaccg atcaattctt
      781 cattgatcag ttggcctagt tccccatgat agctgtctct ttgaatatac ccatcttgca
      841 gcggtgaaat gaagtcccca gcttctttta gaacaccgtc catggtatca aaaataacaa
      901 catctgcggc tttgattatt tctcgtggaa tttcacacat ttccggtgtg taagcaccga
      961 ctccattgat gtgcgcacct tttttgaccc actcagcaga gaatgtcgca cgttttgaag
     1021 tggtcacact tgtaatgatg tctgctcctt caacgcattc ttgactggtt tttgttgggc
     1081 gcatcgttac agaaaattgt tgcatcattt tttcagcaaa ttggctggca cgctcaaaat
     1141 caatatcaaa aatccgaact tcttctaatt tacgaacggt caacattgct tctaattgtg
     1201 actgtgcttg tccgccggtt ccaatcagcg cgccaatttt agcatcttct cttgcaagaa
     1261 gttctgttgc tgctccttga acagcgcctg ttcttaattg tgtcaaataa gtaccatcca
     1321 aacaagcgga taccataccc gtttctggat ccaatacgat catcgttgct ggtacacttg
     1381 gtaagttttt cttgatgttt tcaggataca cggaaacgat tttgacacct agcgcgcctt
     1441 cgccaccttc tacataagca ggcatatata agctttgtcc gttgcttttt ggcacatcga
     1501 tattggtgcg aagaggcaca cttgcctttc ctgctgaata caatgacagt gcttttttgt
     1561 ccgcatcgat ggcttctctc attgaaaaac ttttgataat atcttctttt ttaagaaata
     1621 acatttgaat cgctccttaa ttagctgctg taactttttt taattgactg cccaacatcg
     1681 tatatgcact gtatagtaag accaaaatca cgatccattt caggatatag gtatctaaag
     1741 atttgactag gaatacagcg accaataccc ccaatacacc gaaggttgaa gtaaaaagtg
     1801 tgatttttct gctgtattca ttaaatttaa tgaattgaac actgccgatc ggtactgaga
     1861 aggtacaagc ccccatcata ataggaaagg ctgcaactgg attcaagcct agcaagtaga
     1921 cagttaccat cgttaacgca taagaaccaa tgccgatatt gttcaatgca ccatagacaa
     1981 agagtaaaaa tcctgccaag attaattttc cgccatacaa ttcactggct gttccgtttg
     2041 atggaatcca gttcatttgt cctgcaaaaa tcaacaatgc agcaatgacc aatcctatgc
     2101 cgacaaacag tttgatcgtt ttctctggta acttcacgac aaatcttggt cctaaatatg
     2161 caccgataat ttggcagata atacaaacag ctagtgtctt gatccctacg ttgatggcag
     2221 tgatgtaaga caatgccatg acggctaccg gtactacaca ttgggtattc aacgttccgg
     2281 gtagtttttt catgctgacc catttcaact ttggataaag gacggttccg atagcaaagt
     2341 cggaaacgcc aaatgttgaa agaaagaaga tgataaatga cgtgaagggc aacagacgca
     2401 tatcccctgg ttcttctttg aaattttgtt tgtttttgaa aaggtctctt agaaagtaaa
     2461 taataaaata tagattgatt gcgacaatta gtgtcagcaa aatattccga atcataaaaa
     2521 attcctccta ctagtaaatc tttctgattc cctcaaagcc ctcaccatat tttgctttaa
     2581 gttcttcagg agattctgca atcactttta gtgtatatgc atcatgtttg acatattctt
     2641 ttgctagatt gaccatggcg tggttttttg tccaagtgac atcggttgat tgcagttctt
     2701 cgttaatggt accaaacacg acttcttgct catcggcgac gatgttgatc catcttgctc
     2761 caaagtcagc aagtttgtct gtttcaaaac catgaatata atacttttct aagggaagat
     2821 catattcatg ggtagaactg aataaaatca gtacaaactt atcaactctt ttttcagctt
     2881 gctgcaatgc ctttagtagt gaatccgtga aattctcatg ccaaacttga atgagcaaac
     2941 tttcttttgc gtttttcacc aaatgctcta ctttatccag aacatactcg ataccatcaa
     3001 ctttccacag catttcttct tcatcttttt ctttgatcat ccctagattc ttggtaagaa
     3061 aagaaagatc atttttaaca cttttttcta atttctctaa aaactcattt gccgagatgg
     3121 catgataaag tttaggttct gatttattaa cgacaatcaa attctttttt aatagttttt
     3181 ccaaaatatt atatactttc gagcgtggca cacctgagat tttacttact tcatatcctg
     3241 taagattacc tttttctaat agtgtggtat acactcttgt ctccatctct gaaaaatcat
     3301 attttttcat gatttgaata atcgtttcca ttgattaatc ctcactttat ttttagtagc
     3361 tactacggta acaactataa attctttttg attatctgtc aagagttagt tcaataacac
     3421 acatataata attagcttaa aaacaatctt tttattaaaa agaaacatta taatatgaat
     3481 gtttgtgaaa aaataataca acttagtcat tctttacaac gatttataga aaaacaacct
     3541 ctttaaatat aaaaggtacg ggttactgat tacttgtaaa tctttacgtt ccagttttat
     3601 tctttctaaa aaatatactc acttcttttc ttagacttct ttttttgatt ctcctcttta
     3661 tggtcttgtc cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa agcatttcta
     3721 ggttttggac aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga
     3781 tatcatcaca ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag
     3841 atttcactgt gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta
     3901 atctaagcga tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg
     3961 gaaaaacttt tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttgttttgtc
     4021 ctaccacacg ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg aaaatgacct
     4081 ccaaaaacaa aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc
     4141 gtccgcctcg gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat
     4201 aagataaatc atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg
     4261 taaaaactta tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt
     4321 acccacctgc acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc
     4381 attataatga ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa
     4441 ggcaccaatg aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac
     4501 cctttccttg caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta
     4561 tagcggcaac cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc
     4621 gcaaacccgt agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc
     4681 agtccctacc aaacctgtac tattttttta caggaggttg ctatgttcaa aatctttcgt
     4741 aaaaattgtt gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca
     4801 gatgcgaatg gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc
     4861 aatgaattag ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc
     4921 ggtaaatttt ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct
     4981 catccgaagt acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg
     5041 gatttgtgat ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat
     5101 ccgccagctt cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga
     5161 aaagaatcgt gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag
     5221 tgatgtgttc ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga
     5281 atcatttgat gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca
     5341 agcggcagtt gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa
     5401 ccatattaac gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc
     5461 agaacccttc tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc
     5521 aataacagcg attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc
     5581 gaaacacttt atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac
     5641 aaaatcaaca cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc
     5701 caatgctctt ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa
     5761 agcgaatcgt ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat
     5821 ctggaatgtc ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc
     5881 tgcgaaagaa gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg
     5941 atataccatt atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg
     6001 ttgtcttcca tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg
     6061 tatcttcaaa tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct
     6121 tagcatttaa gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc
     6181 tcctagtata taggcagact gccaattata actgattcaa tccacattat gataaaatcc
     6241 accttttcgt ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg agtcttttca
     6301 ttcagtgctt tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt
     6361 acaaaatcag aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg
     6421 caacagattg gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg
     6481 ctgtttccca gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag
     6541 caattgacta cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat
     6601 caaatcaaaa atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg
     6661 actgtatcat aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat
     6721 tggacagttt tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct
     6781 cgactcttgt ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa
     6841 aatagaaaag agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct
     6901 ttgtggatcc attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg
     6961 tatttgataa tttatcttaa caaccagata attttattca gcaccgtatt atctatattg
     7021 aattctcgga atcaagataa atttttttcc gtttttgttt cttttttaat cttcttcttg
     7081 ttattaatag tgattaggat gatattccta tattgatctt tgataattag atataatttt
     7141 tgaataacgt tccctgctat tcgagctaga aatacaagta cgccatgacc tagttagcga
     7201 caactactta gagatttctt ttgttctggg acaacaaaat gccatgacaa tagcagtaaa
     7261 taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta
     7321 cccaaagcgg ttcaaaaatt ttaaactatt attttaggag atgattttat aaaatcgaaa
     7381 gcctacttga ataaaaaaca agcctgtgaa tttctaggaa ttgataataa tactttagat
     7441 aagtggattc gtgtatataa ttttccagta ataaaaattg atggcgtagt tcgctttgac
     7501 gtagaggata tcaaagattt catgaataca ttcaaaaaaa attgaaattt tcttctcgtc
     7561 acatatacta taaatagcga aatgtactgc cttacttttt aaaggagtga ggaagtttgc
     7621 ctacaaagct ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag
     7681 aaatcactgg aattgcatca gagaagtatc aaaaagtatt tccaactagg caattagcta
     7741 ttaaggccga aagggaaatg aaaaagaaga ttgaaacagt tttaagagag gagaatgcaa
     7801 actcactaga attgaaagga aaaataaaat ttaaagattt ttatgaatca aaatggcttc
     7861 ctagatatga actaggtcaa actactaggt caaacagggc tccaagtaat gtaacaatta
     7921 gtaatactaa ggatatattc cgattgcaca tccttccaat gtttggtgaa tatgcaatga
     7981 attatctgaa ttcaaatact gagataatat cagatgaatt aacaaagaaa tcgaaagaat
     8041 acgcaaatat aaaaattatc aaaggctatg ttcgctctat gtttgatatt gccgaaatac
     8101 tatgttatat cgaattcaac cgaactacca aaattattca atgtattacc gcgcctaaga
     8161 aaatggcttt agaagaaaaa aggataaggg aagggaatca agcattatca tcagaagagc
     8221 tgactgattg gatagaagca gtaaacaatg acttaaataa tcacttgctt acacttcacg
     8281 attacacact tttcatgctt accttatatc taggtgatag aaaaagtgag acttatgctc
     8341 tgcaatggaa atatattgat tttaataaaa atacggttcg tctcaaatat gctttagata
     8401 aatatcaaag aaagacacat acaaaaggtt ggaaagacac cgttattcag gcaccagaag
     8461 ttgtgatgac attattaaga gaatggaaat cagctcaggc tgaacaattg ttgaaactaa
     8521 aaattataca aacacctgac cagtacctct ttacctattc caaaccttct ggagaagtaa
     8581 attgtcctgt tcacgctgac tacttaaact atcgaatcaa tgcaataaaa aaaaggcatc
     8641 ctgaattggc tcaattatct ccacacaaac ttcgtcatac ctatgcaact atagctaggg
     8701 aaggtggagc aaatatgaat caaatctcta atgctttaac gcactcggat atttctacta
     8761 caaaaatata cgtgaacaca cctgacgtag ttgataaatc tgtttttgaa gcatttcaaa
     8821 aaggattgaa taagtgtgat taacatgtgt tctcaaatgt gttcttatta taaaaaaaga
     8881 cactttccaa ataattgaaa agtgtcctga aatattgata taaaaagaat attaacgttt
     8941 tgagaattgt gaagctttac gagctttttt aagacctggt tttttacgtt cgaccatacg
     9001 tgcgtcacga gtaagcaagc cagcacgttt aagcgctaaa cggaagtcag gatcaacttc
     9061 tagcaatgca cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc
     9121 gtttacgtta acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt
     9181 gattacttca cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt
     9241 aattttgcca gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt
     9301 gccgatatat tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc
     9361 taaaacttct ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt
     9421 tgtaaattgt ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat
     9481 caaacgacga gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg
     9541 gtagttgctg tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt
     9601 gtctgcattg atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt
     9661 atttttacca cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt
     9721 tgcgtctact acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg
     9781 cacgttattt tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc
     9841 tctttgtggg gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg
     9901 tttttctgct tgattgaaaa atttatatga aactttactt ctttttcttt cttttatata
     9961 aaagggtcgt ttcctgttaa aataaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac
    10021 aacctcttat tttaaataaa cggcggtctc ctgccaactc ctcggtcaca agtcacaata
    10081 tccataaaac ttaagaagct gttttttgaa attgtttctt gtgactcgga gctgaacggc
    10141 aggtttac
//
LOCUS       DACCSK010000094        10132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCSK010000094 DACCSK010000000
VERSION     DACCSK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.096"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(203..1222)
                     /locus_tag="IVP39_002116"
     CDS             complement(203..1222)
                     /locus_tag="IVP39_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7587180.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGNVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYTFIMDYNTGKELARVRADGIY
                     RPDVNQGYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1233..1430)
                     /locus_tag="IVP39_002117"
     CDS             complement(1233..1430)
                     /locus_tag="IVP39_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7587181.1"
                     /translation="MILPDKYYQVIKWTVLTVLPATSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(1446..1688)
                     /locus_tag="IVP39_002118"
     CDS             complement(1446..1688)
                     /locus_tag="IVP39_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7587182.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(1745..3856)
                     /locus_tag="IVP39_002119"
     CDS             complement(1745..3856)
                     /locus_tag="IVP39_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587183.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLFVNKLESSDF
                     YMGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     NKLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFS
                     NRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSA
                     YEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLT
                     FTPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSN
                     NPKYYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAK
                     DAQTKANANETAIKSTNSRVNEIENIITALTKEETYLLPASIVSGGSLKFTSRGGMTV
                     ATGSFKLAKDIAFGEVLVNDLPDSMINIDNNAIGTGAVTGNSVSQIYVESNTRKVVAN
                     TAMKKDQWFTVGFSYHSKLSI"
     gene            complement(3880..6171)
                     /locus_tag="IVP39_002120"
     CDS             complement(3880..6171)
                     /locus_tag="IVP39_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587184.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIIEGRIIQCYVDENRAKQRLRIYYAKTSVIGNMIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHETRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVNNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKIA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            complement(6181..6918)
                     /locus_tag="IVP39_002121"
     CDS             complement(6181..6918)
                     /locus_tag="IVP39_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7587185.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDAEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFSLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            complement(6968..>10132)
                     /locus_tag="IVP39_002122"
     CDS             complement(6968..>10132)
                     /locus_tag="IVP39_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7587186.1"
                     /translation="STEVQKLERELINQQTAQQRLSNEIDKTSNALAQAKGEIQTYES
                     TMQQLDSEQKNVQASASLIESEYKKWQATAGQSASEAEKLAKAQEYVSQQSENAEKTI
                     DILRRQLEATQSEFGATSTEAMQMEAKLNDAEREFEELGQAAKNVDTTNLDDIGSKID
                     MNNLMEASDVLSDIGDKLTELGKQAVDSANSVGSSQSKIQANFGLSKQEAEELTNVAR
                     DIYYKGFGESLDQSTDALILVKRNLGDLNNQDLQNITEQAMVLENTMGADMDETLRGV
                     NGLMVNFGLSAQDAMDLMVSGTQNGLDKTHELGDNMAEYSQLWSQMGYSADETFGMLQ
                     NGLDAGAYNLDKVNDLVKEMGISLTDGRFEQNMDMFSESTRKAFEEWKNGGGTQKDVI
                     NSMIQDFSNMDGQYDQLNKASTIWSALGEDNAMKVVQSLTDVNHTFDDVSGSAQKMNE
                     DSTTPLQELNGKIAELKDSLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDFIVA
                     IGGLTAAFGILLPAILAVSYLFGPMMLIIGGIIAVIAGVIVAIKNWGAITDWFSGLWK
                     KFTDWLGDTWESIKDGASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGAS
                     NIWQGVQETWQTFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIV
                     GIFDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWW
                     NGIWTAISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGI
                     WNSISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNG
                     ITSTISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRD
                     KISGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMN
                     GVGEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVT
                     MKQTTQQIIDSITKVQNDNASAWR"
BASE COUNT         2913 a         2183 c         1566 g         3470 t
ORIGIN      
        1 gagctaaggg tactggttgt tgccacataa agccataaac aatcaaccag tagaatgtgt
       61 ggcaacaaac ctgttatcgc atagcttgga gtgtgactat ttatgggtga tagtgaagat
      121 atgcgataac atcactattt tatcgaataa tttttatagt tgtcaatata gttatgtact
      181 gctcctcaac gaggagctat ttttatcgtt taggaatatt taaataccaa cgtttgtcat
      241 ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc attcgttgcc cgcatcatta
      301 catagacttt cttactagga aaattacgca tattgaaaga tacatgataa ccaacgtttc
      361 cagaagtatt ataaccttga tttacatctg gtctataaat tccatcagct cttactcgag
      421 ctaattcttt cccagtattg tagtccataa tgaaagtata ctcgtattta tagttagcaa
      481 tgtgccatcc agccacatgc aagtttgcgt tttcgatttc tccaaactga tcaatgtggg
      541 cgtaatttgt tccatctgtt agtgtaggat ttgcagcacc tgctcgagtt ggatcaatta
      601 ctggtttatc ctccgaagta gttggattat catccgtaaa accatgagcc aaatcatagg
      661 ctagtttttc tttacttaca cccatttcag aaagataacc gtaaggatct gtatgatcgc
      721 cccaaatatt ttgtgttacc cataaatgcg atttgattcc tggttggtta taaggagtgt
      781 ctaatgttaa tggaatacca tatttcattg ctgaatctct agctaattca acgtatgcct
      841 tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg taactcaatc tgcacgggac
      901 tgttggcatt agcatacgaa ccagccccat actgcacata accaggttga ccgacttgat
      961 aaacatttcc gccgtctccc acaatgtaag cagtgtaagc actagtccat gaacgttgca
     1021 tatactgtgc ttcattgcgt cctgttgctg tttcgttagc tgtttcatgc agtaaaatat
     1081 actgattatt cgctacttga gagctacctt cgtttggccc taaattaaat tcattgttaa
     1141 tcgtataggc aaacccatta attggcaata aaaaaagagc cgttaatagg ctcatcgcag
     1201 taatagtaat tttctttttc atttgtttcc tcctattttt tcaaattata agccgacaca
     1261 ccagtgataa cacctaaaaa tgttgctgct gcattgatag tcagaactgc catatctgtt
     1321 ccattccatc catatgcttt gcctagtgtt gcaactaata cagatgtagc cggaagtacc
     1381 gtaagtaccg tccatttaat gacttgataa tacttatcgg gtaaaatcat ttcttctcac
     1441 ctcctttaca atttagtcaa gaaatagcca atgatcgtaa tgcctaaacc gatcatgtaa
     1501 ccccacgacc atttattatt ggctttcatt tctttgatat cttccgcatt attaagcgca
     1561 atagaatatg cctgatccgc tctatctttt gcactttccg ctttttcgcg taatgattcg
     1621 taattatcca acttcgtttc aatacgcact aaacgttcta ccacgtcttg tattgcttca
     1681 tctttcaacc aactagcctc ctttcattgc aaaataaaaa cgtactcgaa tgagtacgct
     1741 aaatctatat agataattta ctgtgatacg aaaatccaac tgtaaaccat tgatcttttt
     1801 tcatagcggt attcgcaacc acctttctcg tattagattc aacatatatt tgcgatacag
     1861 aattacctgt gacagctcca gtccctatag cattgttatc aatattaatc attgaatctg
     1921 gcaaatcatt aactaaaact tctccgaatg ctatgtcctt agccaactta aatgatcctg
     1981 tagctactgt cattccacca cgacttgtga atttaagaga tccgccactt actatactag
     2041 caggtagcaa atatgtttct tcttttgtta gggccgtaat aatgttttct atctcattaa
     2101 ccctactatt cgtactcttt atagcagtct cattagcgtt cgcttttgtt tgagcatcct
     2161 tagctgtggt atctacttca ttaattgaag ctgtcaactg cgaattaatc tccgatactt
     2221 tcccatcggt ataattgttt gctttacctg tgatttcagc aaccttgata tctgtagcta
     2281 ggttattctc aacatagcta ggttctagat cccatacata atattttgga ttgtttgagt
     2341 ctcgcatgcc gattcctcgg tatttatatt gctcaatatt cggggttcga gtgtcacctt
     2401 tttcaatctt gagccagtca attcggcatg cgccggccgt ttctttaggc gactgaaaaa
     2461 tgcgaaggtc tttaggcaaa cccggttcaa gtttcgttgg tgtgaatgtt agagaccata
     2521 cgtctgtcaa tccctcaact ggttttagat ctccaaaatt aacgttccaa taattatatg
     2581 ctacaaaggt ttgacttgcg ggttttgttc ctttaagcgt gatagtatac gtttgaccta
     2641 ttataagctc ttcttccgtg ttacctttat atatttcgta cgcgctagat ttaattggga
     2701 acgtcgtacc tttatttgca atattcttac ccaacgatgt tttacctacc cagtaagggt
     2761 catctagtaa gtttggctga tacggagtgg ctgttgagcc ttcttcgatt ttgatgtcgt
     2821 acaatttaaa tccaccattt attttatccc tgttagaaaa actaacacta atataaaaac
     2881 gatctaaatt tgtgatttga taattaacgt tagctgtgcc tttgattgtt atttctttcc
     2941 ctacatcatc tattgtaata gttgtattat ttgcttccaa taatatattt tctcctggtg
     3001 atgtacgata taccaaacgt aatttattaa tagctcctgt agttccttca tcaaatcgaa
     3061 cttttgcact cagagtatat gttttgccac tagcaagctg aggtgtatta atacgcgtaa
     3121 acatatcaat gtcccctgta ccatcagaca taaaatgtaa cattccgtta acattagtta
     3181 gtgacccatg gtaacccata taaaaatcgc tagattccaa tttatttaca aataagttgg
     3241 gattcccact ataatcatag tcaccgaagt cgatgctgtt actgtacatc acttgtaagt
     3301 tacctaactt agaaatttct tctttcagag catctaactt gtcttgtagt gttttagctt
     3361 gaccagttaa atcagtaatc tgttgattta agctatccac tctaccttta atttcagcca
     3421 taaaagcatc aaaagtttcg ttatactttc gaatcaactc ttccaattgc gaaacgtatt
     3481 catcagcttg gccttgcgaa atgtcagaca ctcctagtga gaaaaaaatg atgtcttgcg
     3541 ttgttaaaat ttgattacct tttctatatt ctacgtagca gtgtttataa tatcctgctt
     3601 cactcataaa tgtgccatca agagaaaatg tgacttcttc actagttata ctagttgcaa
     3661 cactatctac ataacggtta gatggcgttg ttccttttaa agtaaatgtt ccgccactcg
     3721 tatccatctg caagccattt aaaaatggtt taacagtcac cgtaattcct ttgtcacctt
     3781 gacgagccat aatagctttg gtgtagttta attctttact gaaatctaga gccaaattat
     3841 ataaactgct agccatttat atacctcctt gtcttcgttt taaaaacgtt tttggtcaag
     3901 cactgtgcta tcatatgctg tatcctcttt taatctaaca tcttcatacc ctagtcgatg
     3961 tgccactaaa ttccatctaa ccaatacgtt tggcttacta gtttcaatga tgaaatggtc
     4021 aacatcttca tgagtaacag cacacaaaac tagttctgta ggtgtcacat gtgtcatata
     4081 ccgacttaga tttactgtct cagcaaacat gggatcaata tcaacacgaa ctttaccatc
     4141 atcacctgta acggcttctc cataatcagc gaaataatat tctggagttt cataagcgtt
     4201 caatagtcgt tgtccataat gttctgtcgg tacggttgag tttttagtac ctctaacagt
     4261 aaaattttta tatacttgta ccgttgattg ttcaaacctt gcaattttcc catcttccca
     4321 tgaaccaaaa aaacaacctg gcaacgttag cataccatca ctagtaaatt tcatagtcct
     4381 gccagctact ttaaactccc atgaactacc cgcactacca ttaatgctta aagaactacc
     4441 gtcgccatca gttatataac tagcattgct atacctgaaa ttgggcgcac caaaagatag
     4501 aaacggtttg ttattgccat tatcccacgt actaaaaacc aaattcccct gtggatttct
     4561 aatcatgaaa ccaccaccag ttttcatggt gtatgataca ataccggcat cagcacttac
     4621 ataatcacgt gcttctagct ccataatatc tttgttaact ttttttgagt accaagtcat
     4681 tttgccattg gcaatacttg ttctataatc agtaccatca ctaattaatg tagtacctct
     4741 aatagtaatc cctactatct caccagctgt aataaacgag gcattaaatc cgccatctaa
     4801 cgtccatgcg gtgtcatacg ttccattaat accagtttta gagaaaccaa taccagcatt
     4861 gttgatttgt aaaacattcc ttgcggtatt cttatctggc gtgtccataa tcaaaatacg
     4921 actaggtgct tctttaggat ctagtaaaac atacccacca tcttgaccag taatcatgtc
     4981 agtttgatga tctacaatat cattgattaa atcactgatt tcgccaccat ttttcaattg
     5041 atcaatggca tcattaatca aattgttgac attattctct gtattttcta agaagtttgt
     5101 tttgacgttt cctacaacta atttatcgta tgaattggtt aaaacattaa acgtatattc
     5161 cacaattctt gctgacatat tcacttttaa ctgtggatga tacacatcta ctccgtcacc
     5221 catcgaaact ttttctagat caacaaattt ttcatagcct ctttgatgcc tcaatggtac
     5281 taattcaatc gaaccactca cttgtggttt ctgtttatct atgtttgttt tcaaccagtc
     5341 tttagcagct tcccttaatg tggccacatc agtcgctttg tctttaaaat caacaaaaga
     5401 aacatatcca gcaggataat cttccacgta atccgtgaaa atgacttctt ctggtagagt
     5461 gatctcatcc tctccttctg acgaactgct aatgaatgga taaactccaa ctaaaacact
     5521 ttgagcatct atctctaagt cgagaccagt taagttttta gtataaatcg ctttgatttt
     5581 atgatccgta cctagccttg tttcatgacg taatgtgtta ttatctttaa gaaattcccc
     5641 atgaaatcga tctaggatag atccttcctt tccgccaaag aattctaaaa aattcgcctt
     5701 ttctatcttc acattagcaa gcgtatctac taacgatgag aaagaaaact gcgaaggaat
     5761 agctggtttt gctaaaactt ttgcgttttg ccatgcctga gtagcagtga tcttttctgt
     5821 tccgctgtca tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt
     5881 tacttctatc atatttccta ttacagaagt ctttgcataa taaatccgta aacgttgctt
     5941 tgctcgattt tcatccacat agcactgaat aatacgtcct tctataatca agtctgcatt
     6001 agttccgctt attggataag taccctgaaa tatctcggct ccgtttaatt tattgctaac
     6061 agtggctgtt aaccagtctg acaaagcgcc taaaccttgt gtatcatata aatgttcagc
     6121 taaattattc gcgtcatttt tatcgtaaat agttattaaa ttatcgatca tctatttcac
     6181 ctaccttaac ccattacgat aaatttgtat tttgctcaaa ccagtacaat taaaataatt
     6241 aatatccact tgcaatgtcg gatattgcat ggtcttcatt ttgttggatc gatctaaaat
     6301 atctccgtcc gattgctctt cgtagcaaag cattaaatca ctatcaatga ctacatcagt
     6361 tcctactact aagccttcaa aactaaacac ataatcattt aagatgaact ggcatgaagt
     6421 agctgaagga gtgatgataa tctttggaaa actttcttct aaactattat tcagcaaact
     6481 aaatgactgt ggtttatcta cggttatagg cacatcttct tgaactcttg cgaatggttt
     6541 cgcagtaata tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat
     6601 atcgatagtc tggataacat aatagacgtt gggatcatca gagaattcta atttctttgc
     6661 atagtttaac caatgacgca tgatataaaa cgattgcttg aacgcttgat ggtcttccgc
     6721 atcctctaaa tagttatagt gcaaagtaaa cgacatatct tcaaacgaat aatcttgtac
     6781 taagccacct aaccgtccta aaacagaagt ttcaactctc tgtctttttg gagatggtat
     6841 ggttggtctt tcagctaaag ccaatttatg caaataatca ggaaatccat cgattataga
     6901 atgtatacaa tcagtcattt tttcacatcc tttttaatac taaaaaacag gagaaatact
     6961 ctcctgttta acgccatgcc gaagcattat cattttgaac ttttgtaatg ctgtcaatga
     7021 tttgttgagt tgtttgcttc atagtaacct catctgcgtt accatcaatt gtgaaattga
     7081 attcgtagtt attcacaggt tgaatcgttt gtgccctaga tgaaactgat gtgctactca
     7141 agatacgatc accaatttct tgcaaaacag atcttttcaa aggtaaaact gcttcaggtc
     7201 ctgcttcacc gacaccgttc attccaccta gtaaagttgg tttagtaaag atacctcctt
     7261 tagcatgcca ttttacacgc aaatggggga tttgaccttt tagcgggtta aagctgcctt
     7321 ccatgataaa ttccggtaac ggaatatgtg gtatagaaat attcaaatta tcaaaaatgc
     7381 cactgatttt gtctctaatc caatcaattg gagcgctaac agtctttttg atgccttccc
     7441 atatgttagc aattgtactt ttaacattat tgaatatgtc ggaaacaata cctgttagat
     7501 tggaccaacc gcttgaaatt gcattttttc catcgtttac tttagagcta atagtgcttg
     7561 taattccatt ccaaagattc aaagcagtat ttttgatacc gttccaaatt ccgctaatcc
     7621 acgaagatat actattccaa acactttgaa tggcactttt agctgcgttt atagcattgc
     7681 ttatactact agtcacacta ttccagatat ttgatgctgt agagctgatt gaattccaaa
     7741 ttccacctaa ccaactagat acagttgacc aaatattttg aattactgta gcagctgctt
     7801 gtaccaagct agtgattgta ttcttgatac tgttccaaat actagaagct gttgcactaa
     7861 ttgaattcca aatatttgaa gccgtagtac taatagctgt ccatatacca ttccaccatg
     7921 ccactactgg atcaaatata gtatggaatg tagttacaat cccattccaa gcgatactta
     7981 tccattgtgt catagtatcc caagtatttt taaggaaatc agaaataggt gtccaaacag
     8041 cttgccaagc tgcgcccaat aactgtccag ctacatcaaa aatacccacg ataatattaa
     8101 taccagcttg aatcaatgac gttattaatg tccatggtat ttgaacaatt cctacaatgt
     8161 ctgcccaaat aatcgaccat acttctttga ctccgttcca aatatttgaa acccaatcaa
     8221 cgaatgtttg ccaagtttct tggactcctt gccagatgtt ggaagctcct tcaactaatc
     8281 cgctccatag ctctccaaac caatcagaaa ctccttgcca aatatcttga acccaatcta
     8341 caaatccaga ccaggtttct ttaaccccat cccaaactga tgaggctccg tcttttatac
     8401 tttcccaagt atcacccaac caatcagtaa attttttcca taaaccacta aaccagtcag
     8461 taattgcacc ccagttctta attgccacaa taacgcctgc tataacagct ataattcctc
     8521 caattataag catcatcgga ccaaataaat atgacacggc tagtattgcc ggtaacagta
     8581 tgccaaatgc tgctgtaaga ccacctatag caacaatgaa atcctgtact ggttgtggaa
     8641 gattattaaa cgcatcagcc atctttccta gaaaatcaat tactggttcg agtgcatcta
     8701 tgattgtgtt gcctatagga gctaatgaat cctttaattc agctattttc ccgttcaact
     8761 cttgcaacgg agtagtagaa tcttcattca ttttttgtgc agatccacta acatcatcaa
     8821 atgtatggtt aacatcagtt aaagattgga caactttcat cgcattatct tcgccaagcg
     8881 cagaccaaat tgtcgaagct ttatttaatt ggtcgtattg accatccata ttgctaaaat
     8941 cttgaatcat ggaattaata acgtcttttt gtgttcctcc gccatttttc cactcttcaa
     9001 aagcttttct agtactttca ctaaacatat ccatgttttg ctcaaatcga ccatctgtta
     9061 acgatattcc catttcctta actaagtcat tgactttatc aaggttataa gcacccgcat
     9121 ctaaaccatt ttgaagcatt ccgaacgttt catcagctga atatcccatt tgactccata
     9181 attggctata ttctgccata ttgtcgccta attcgtgcgt tttatctaaa ccgttttgag
     9241 tacccgaaac cattaaatcc attgcatctt gagcactcaa gccgaaattg accattaagc
     9301 catttacacc acgtaacgtt tcatccatat cagcgcccat ggtgttttct aggaccatag
     9361 cttgttccgt gatattttgt aaatcttgat tatttaaatc tcctaaatta cgctttacca
     9421 aaatcaatgc atctgtggac tgatctaacg attctccaaa acctttataa taaatgtctc
     9481 tggctacatt cgttaattct tcagcctctt gtttagacaa accaaaatta gcttgtatct
     9541 tactctggga actacctaca ctgttagcag agtccactgc ttgtttccct aattctgtaa
     9601 gcttatcgcc aatgtcgctt aaaacgtcag aagcttccat taaattattc atatctattt
     9661 tgcttccgat atcgtccaag ttagttgtat ctacattttt agcagcttgt cctaactctt
     9721 caaattcacg ttcagcatca ttaagcttcg cttccatctg cattgcttct gtggatgtag
     9781 cgccaaattc agactgtgta gcttctaact gtcgtcttag gatatctatc gttttctccg
     9841 cattttcaga ttgttgagaa acatattctt gggctttcgc taatttctcg gcttcagaag
     9901 ctgattgacc agcagttgct tgccattttt tgtattcgga ttcaatcaga gaagcactag
     9961 cttgaacatt tttttgttcg ctatccaact gttgcattgt agactcgtac gtctgtattt
    10021 cgccttttgc ttgagctagt gcattactcg ttttatcaat ttcgtttgac aaacgttgtt
    10081 gcgctgtttg ttgattaatc agttctcttt caagtttctg aacttcggtg ga
//
LOCUS       DACCSK010000095         9992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCSK010000095 DACCSK010000000
VERSION     DACCSK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.097"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(338..1291)
                     /locus_tag="IVP39_002123"
     CDS             complement(338..1291)
                     /locus_tag="IVP39_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7587187.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="IVP39_002124"
     CDS             complement(1314..2069)
                     /locus_tag="IVP39_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7587188.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="IVP39_002125"
     CDS             complement(2066..3025)
                     /locus_tag="IVP39_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7587189.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTFLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="IVP39_002126"
     CDS             complement(3018..3968)
                     /locus_tag="IVP39_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7587190.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="IVP39_002127"
     CDS             4118..4570
                     /locus_tag="IVP39_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7587191.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            complement(4658..6925)
                     /gene="gshAB"
                     /locus_tag="IVP39_002128"
     CDS             complement(4658..6925)
                     /gene="gshAB"
                     /locus_tag="IVP39_002128"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7587192.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7112..8110)
                     /gene="ftsY"
                     /locus_tag="IVP39_002129"
     CDS             complement(7112..8110)
                     /gene="ftsY"
                     /locus_tag="IVP39_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7587193.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8122..8931)
                     /locus_tag="IVP39_002130"
     CDS             complement(8122..8931)
                     /locus_tag="IVP39_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7587194.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8940..>9992)
                     /locus_tag="IVP39_002131"
     CDS             complement(8940..>9992)
                     /locus_tag="IVP39_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7587195.1"
                     /translation="ATERQEAIEKQLATLTADFSDHEVTEESLEKQINELSAQRETLK
                     AELAKAKEQRDRRQKEIDKLEAVLAERNREQKARLSEQSKLEVQKDRAEMILDNHLTY
                     LQSEYQLTFEKASQDYQETTDIEDSRTKVSSLKEQIEKLGPVNLNSIEQYEQVSERHT
                     FLATQRDDLLAAKNQLFETMDEMDDEVRTRFKEVFEAIRQEFKIVFPNMFGGGKAELV
                     LTEPTDLLKTGIEIEVQPPGKKLQSLSLLSGGERALTAIALLFSIIRVCPVPFCILDE
                     VEAALDEANVKRFGRYLSDFQDDTQFIVVTHRKGTMVAADVLYGVTMQESGVSKIVSV
                     RMEDINEEAKINETKV"
BASE COUNT         2917 a         2185 c         1699 g         3191 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt cacgttgaaa
      181 aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc tgattgattt
      961 tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt ggcacctcga
     1141 tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta ctgcttttct
     1201 ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc gtacaattcg
     1381 tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa ctgcccatct
     1441 ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggaa cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa aacgaaaaaa cacataaatt tttacatgca aaaaagcttt tgatcagatt
     4621 gacatcaaat aggacaatct gtcaaaagct ttttctttta aaatacttca ggatataaca
     4681 atttcaatac attcatcgtc aatcttctgc cttttcctgc aaagggataa acatgcatat
     4741 gcatggctgg attgaaattt gcttcgatga tcccatatgc ttttttatca gttgtaggat
     4801 cgatttcttt atctggtata atcaaatcga taccactaat cttagctcct agtgcttcta
     4861 ccgcagaaac ggcgattttt ttgtaagctt cagaaaattc atcagtcata tcgatcgaat
     4921 caccacctgt actgatattt gagttctccc ttagataaac aatttgattg gcttggggaa
     4981 ctgattcgat cgtcaaccct tgttctttca acatcaattg ttctaattca cctaattgga
     5041 tcaattctaa aggtgcacga tgattcgttc cacgcaaagg gtcactattt ttttcttcaa
     5101 caagttcttt cactgttcgg ataccgtcac caataacatt agcagggact cgcaacatga
     5161 tggcttggac ttctccgtcg atcacaaaga aacgatattc cgttcccggc ataaattctt
     5221 ctactaaaac cgaactgtct tcacgaaaag caatggctaa acctgcttga taatcttcca
     5281 gactagctcc ttccttgaaa atcgtgatac ctaacccgta attggttgat ttaggtttga
     5341 tcacaaatgc ctgctcggca aatctcggat aagcttttac cgcttcttcc atggatgaaa
     5401 attctgcgcc tcctggtacc cggaacccag cttcttttaa gacttttttc gttactgttt
     5461 tgttttccat gataagtggg acgatatagc tgtccttact tgtcatgttt gcatttttca
     5521 catattctac atggtcttgg aattgcagac gcaaaaattg atccgactca tctaatactt
     5581 tgacttggac acctttttgg atggcatcga acataaatat ctgagtagaa agttccattt
     5641 cgcgaaaacc ggccaactga tagggacgtt catggctttc tttataatat tgatgtccca
     5701 atgccacagc aacttcttgc tgactcgata gctgtgcttc ttgatagagt ttcgctgcta
     5761 acgtttgttc aggatgatcc atttgagtca atgcttcctc tactaaataa agagaatcca
     5821 accaatccaa ttcaaccagc atttgcttca tcccttcaag aatttctcta ccttcttgat
     5881 gaaattctgt ttgatctgat ggttgttcta gtgctacttt gttatttcga gtcgtacctt
     5941 ctgctaccca ttggtcacag tcttcttttt cttctgtcca taacatgtac atcaggaata
     6001 attgtaagaa ccgaacttgc tctggattga tacccaatct tgcataagga ttcaaatcga
     6061 tattccgcaa ttcaatataa cgaacacctg ttgttgctaa atctgcgact ttctttccgc
     6121 ctcggaaacg aagcggtgta taaaattctt tctcctccga aagtttccct tcttcgatca
     6181 ttcgctcgat atctgctaaa tactgtttca tagaagcata ggaaactttc acatttggat
     6241 gattcgtata gcccagcgca ctgttacgaa tactgcgaac aggttcttgc ggatgtgatt
     6301 catctaaaaa gtagttcttc tcactcattg gtgaagcacc gaataaataa gtgatcatcc
     6361 agcgatagcg cataaagttt ctcgccgttt tcaaataaag ttctgttttg aattcggaaa
     6421 aatcttgaaa ttcttcctga tggctgaaca atgttcttag caactcatcg ccaaattcaa
     6481 aattgaaatg gataccgctt accatctgtt ttcgcttccc gtattctttt gctaaataac
     6541 gtcgatacaa gacatcttcg aaatttttta attttgcaat aatgatcgct tcgtcttttt
     6601 ccggtaaggc aggtggcata cttaatggcc agatcatctc ttctttcggt atagaacgag
     6661 cagtcacatc ataaacagca gcgagatact gaaataattc gggaatagaa tcggtaacag
     6721 gagtgatcat ctctgtttgt gtttcgctaa aatctgtttg gatatagggg tgataggatc
     6781 gatcgccaaa gattgctgga tgatcggttt ttgctagatt tcctgcaaga tcaacccgct
     6841 gtccttcccg ttctatacca aatcgagctt ggtcgataaa agggcgcgca ttgacatgca
     6901 ataataattg cttaaaattc atcattaagt gacctccaat tcatatgaat ctaattataa
     6961 tcaaaaagtc taagaaaaag aacgaaattg tttgagaacc gcttacaaat aagtataaaa
     7021 atgaaggcat caaaaacaca gctatgttaa aaacaatcat tcaaacaagg agctgcaact
     7081 tttgtcacag ctcctgcttt ttagacatgg gttattcttc ttttagtaat cctttgaaca
     7141 atccaacgac aaaatcattt ggatcaaacg gctccaagtc atcgattcct tcaccaagac
     7201 cgaccaattt cactggtaaa tgcaattcat tacggatagc aagtacgata ccgccctttg
     7261 ctgtaccatc taattttgtc aaaacaagac cggtcacatc tgttgtttct ttgaattgtt
     7321 ttgcttgaac catcgcgttt tgaccagttg ttgcatctag tacaagaagt gtttcttggg
     7381 gtgcttctgg taattcccgc tggattactc gttttatttt ttctaattcg ttcatcaagt
     7441 tcactttatt ttgcagtcgg cctgcagtat caactagtaa aacatctgcc tgctctatct
     7501 ttgctctttc caaagcatcg aatacgacag ctgctggatc gccgcctgca ttgccacgaa
     7561 caacttcaac accagcgcgt tcgccccaga caactaactg gtcgattgct ccagcgcgga
     7621 aggtatctgc tgctgccatc aatacttttt taccttcgtt tttgaactga tgggcaagtt
     7681 taccaatact tgttgttttc cctacaccat taacaccgac aaacaaaata actgttagtc
     7741 cgtcttcttg gatatttagt tcatttactt cattgattcc ttccgcttca tagagatcca
     7801 caagtttctc gatgatcgca ttttggactt ctgccgattt tttcgcattt ctgagtttca
     7861 cttcttgacg taatgcttcg gtgattcgta tcgctgtttc aaaacctaca tctgcaccga
     7921 taagtgtttc ttctaattct tcaaaaaagt cttcatcgac tgttcgaaaa ttggcaaaca
     7981 actcattcat ccgttcacca aatgttttac gcgtcttttc taaacccttg tcgtacttct
     8041 cctgtacttc aacttcttct gttttctctt cgccagtaaa agcacgcttg attttatcaa
     8101 aaaatcccat ttgactcccc cctactctaa tacaaatttt tggatcactt tggctacgcc
     8161 attttcttca ttgctggctg tcacgaaatc tgctgcttct ttgacgaaag gcacagcatt
     8221 ctccatcgct acgcctagtc cagcgtattg gatcatcggt aaatcatttt cttcatcacc
     8281 gattgccatg atttcttctt gtttcaaacc aagatctttt gccaataaag aaataccata
     8341 agcttttgta acaccttttg gcataaattc caaaagcata cttctcgttt tgatgatctc
     8401 atacatttca tggaattctg ctggtatttc tttgatttta ggatctaatt cttcttggtc
     8461 ataagcgatt actgccttat taagcacaaa ttcatctgta atatcgctta tatccgctgg
     8521 ttggaattgc agcagcttat tcaacacatt gtacaaggat tgcttccttg gggcactagg
     8581 cagctgtaaa accacattgt cagacaaaac atctagtggc agatccaatt gctgagccag
     8641 tttgtataag cgttggatat ctgatacttc catcactttc ttttccatga tttcaccagt
     8701 atcatttttt tgtaccaaac cgccgttgaa agtgatgctg tagtcacctt catctaccag
     8761 tcctaattct tgcagataat gcgccatcgc agctaagggg cgtcctgtac atagaacgat
     8821 cttgacccct tgctgttttg cttgtgctaa tgcttgttta ttctcatctg aaatcagctt
     8881 ttgttcattt aataatgtac cgtccaaatc gattgctact aatttgatca ttttcgttcc
     8941 tacactttcg tttcgttgat tttcgcctct tcattgatat cttccatccg tacagaaacg
     9001 atttttgata caccggattc ctgcatcgtc actccgtaca gtacatctgc tgcgaccatc
     9061 gttcctttgc ggtgtgtgac tacgataaat tgtgtgtcat cttggaaatc gctcaagtaa
     9121 cgaccaaaac gtttcacgtt ggcttcatct aacgctgctt ctacctcatc taagatacaa
     9181 aacggaacag gacagacacg gataatagaa aagagcaacg cgatggctgt aagtgctcgt
     9241 tctcctccag aaagcaaact taaactttgc agttttttgc caggaggttg tacttcaatc
     9301 tcaatacctg ttttcagcaa atcagttggt tctgtcagta ctaattcagc tttaccgccg
     9361 ccaaacatat ttggaaagac aattttgaat tcttgacgga tagcttcaaa tacttctttg
     9421 aatcttgtcc gcacttcatc atccatctca tccatcgttt caaacaattg gtttttcgct
     9481 gctaacagat catctcgctg tgttgctaag aatgtatgtc tttcactgac ttgttcgtat
     9541 tgttcgattg aatttaagtt tactggtccc agtttttcaa tctgttcttt taaggatgaa
     9601 acctttgttc tactgtcttc gatatcggtt gtttcttgat agtcttggga agctttctca
     9661 aatgtcagct gatattctga ctgcagatag gtcaaatgat tatctaaaat catttcagct
     9721 cggtcttttt ggacttctaa tttggattgt tccgacagtc gtgctttttg ctcacgatta
     9781 cgctctgcca agactgcttc aagcttatcg atctcctttt gccttctatc tcgctgctct
     9841 ttggcttttg ccaactcggc tttcaatgtt tctcgctgag cagataattc gttaatttgt
     9901 ttttccaaac tttcttctgt gacttcatga tcagaaaagt ctgctgtcaa tgtcgccaac
     9961 tgcttttcta tcgcttcttg acgttcggtt gc
//
LOCUS       DACCSK010000096         9711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCSK010000096 DACCSK010000000
VERSION     DACCSK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.098"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1421
                     /gene="lsa"
                     /locus_tag="IVP39_002132"
     CDS             <1..1421
                     /gene="lsa"
                     /locus_tag="IVP39_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7587196.1"
                     /translation="DTQWKLGLIGRNGRGKTTLLNILQNKLPYQGQVIHQQEFAYFPQ
                     QTKDKERLTYYVLNDITDFEIWEIERELQLMQTDPEILWREFSTLSGGEKTKVLLALL
                     FVDDTHFPLIDEPTNHLDISGRKQVAAYLKKKKQGFIVVSHDRGFIDEVVDHVLAIEK
                     SQLELYQGNFSIYEEQKKLRDEFEMAQNEKLKKEVSRLKKTAAEKAEWSRSREGDKTK
                     KQVGFIDTESRRVNKGAVGADAARTMKRSKAIVNRMETQISEKEKLLKDIEYIDSLTM
                     NSQASHHKRLLSVEDLQLGYENLLFEPIHFTIEPHQRVAISGPNGAGKSSIIHYLLGA
                     FNGKVIGEKYQPKHLSISYASQNYEDNRGTLAEFAEKNQVDYQAFLNNLRKLGMERDV
                     FHNKIEQMSMGQRKKVELAKSLSQPAELYTWDEPLNYLDVFNQEQLEQLILNVKPAML
                     LVEHDQTFLDKVSTEIISLERI"
     gene            1488..2015
                     /locus_tag="IVP39_002133"
     CDS             1488..2015
                     /locus_tag="IVP39_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7587197.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2226..3287
                     /locus_tag="IVP39_002134"
     CDS             2226..3287
                     /locus_tag="IVP39_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7587198.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3290..4186
                     /gene="murQ"
                     /locus_tag="IVP39_002135"
     CDS             3290..4186
                     /gene="murQ"
                     /locus_tag="IVP39_002135"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7587199.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4200..6182
                     /locus_tag="IVP39_002136"
     CDS             4200..6182
                     /locus_tag="IVP39_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7587200.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6247..6693)
                     /gene="nrdI"
                     /locus_tag="IVP39_002137"
     CDS             complement(6247..6693)
                     /gene="nrdI"
                     /locus_tag="IVP39_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7587201.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            6904..8328
                     /locus_tag="IVP39_002138"
     CDS             6904..8328
                     /locus_tag="IVP39_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7587202.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(8509..9282)
                     /locus_tag="IVP39_002139"
     CDS             complement(8509..9282)
                     /locus_tag="IVP39_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7587203.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9279..>9711)
                     /locus_tag="IVP39_002140"
     CDS             complement(9279..>9711)
                     /locus_tag="IVP39_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587204.1"
                     /translation="LDPQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILIN
                     QGKIIKQCTMAELNQTAQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELL
                     GTVIQLLSAQNIHVTDVQHLTNDLESSLLEILDSVKGEEEK"
BASE COUNT         3107 a         1707 c         2133 g         2764 t
ORIGIN      
        1 ttgacacaca atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac
       61 tgaatattct acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg
      121 cctatttccc gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata
      181 ttacggattt tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg
      241 aaatcttatg gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg
      301 cacttttatt tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg
      361 atatctctgg tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg
      421 tggtcagcca tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa
      481 aaagtcaact ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc
      541 gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga
      601 aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac
      661 aagtcggatt catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg
      721 ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg
      781 agaaagaaaa actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag
      841 cgtctcacca taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt
      901 tattcgagcc aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta
      961 acggtgcagg aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta
     1021 taggagaaaa ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag
     1081 acaatcgagg aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt
     1141 tgaacaacct ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga
     1201 tgagtatggg ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac
     1261 tatatacatg ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac
     1321 aactgatctt gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg
     1381 ataaagtatc tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac
     1441 aacaaggtat ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa
     1501 cgagacacgg agaaacaact tggaacgcac aaggattagt ttgcggacgg gcagatgtgc
     1561 cgctgactga aaaaggacag atgcaggcac agaaactagc agaaaaggta gtagatttgc
     1621 ctgttccaat cacgaaaatc atccattccc ctcttcaacg agcaagagat acagcacaag
     1681 cagttgctga tcgtctcagc ttgccgctga ccgttgatga acgattggta gaaatggatt
     1741 ttggggatta tgatggactg ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt
     1801 tcgctgtacg attcccaaat ggggagtcag tcttagatgt gtatgcacgg atcgttcctt
     1861 tattgaaaga atgcatcgaa gacgaagaaa atgtctatct gttagtttgc cataatgcct
     1921 tgataagagt gatcaatgct tattttcatc cgatgcctaa tgaggggttc tttacattca
     1981 tggtagataa tacagaattg gtttcttatg agtaattatg tttttgtgtt atttaaataa
     2041 aatatttcaa ggcactgctt ttgcttagca gtgccttttt taggttatga caattctttt
     2101 gataatataa actcaacctt tagaaatgaa atttaattat tttaataaaa gtattgaaat
     2161 tttgtttctt tagatatata ctctagtcat agaaatgaag acggatacag aaaaagaggg
     2221 ataatatgtt tggcttttca atatttatga acgattcttt gtcagaagag aagaaacagt
     2281 atatccgcca aatggcatcc aatggctttg tcggtatttt cacttccatg catataccag
     2341 aagatgatgt ttcggcatat caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga
     2401 ataaactaga attgatggtc gatatttcag gagaggcatt ggaacgagcg ggtttttcat
     2461 tagaagatct aacgcctttg aaagcaattg gtgtaacagg tttaagaatg gattatcata
     2521 tttccaatca gcagattgcc gactggtcac atcaattgaa aatcagtttg aatgctagca
     2581 cgatcacgcc taaagatatc gatgaattga aagaagcaga agctgatttc tcacagattg
     2641 aagcttggca taattattat ccacgtcccg aaacagggct ggacaaagaa tggtatcaga
     2701 aaaaaaatca atggctcaaa attcaaggtc tccttgtcca aggttttgta ccaggagata
     2761 cagagttgag aggcccacta tatcaaggat tgccaacttt ggaagaacat cgagggatcc
     2821 atcctcttgc aggcgctcta gatctattag caagtaacac ggatattgtt tacatcggag
     2881 atgcaggtct gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa gaacagacag
     2941 tcttgctcca tacggaacct gtagatgaag aattttacga atatatatta gggaagcata
     3001 cgaatcggca agacgatgca agagatgtca tacgaagcgt agatgcacgt tttcgagaga
     3061 ttccgcctat cccagcaaga aatacagcta cgcgtatgaa agggtctatt actttagata
     3121 acgaaaagta tttgcgctat atgggtgaga tccaattaac caaatatgat ttgccagcgg
     3181 atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga gctaccattg atcaaccaaa
     3241 tcaaagctgg tatgaactat caatttataa gaaaagaagg aagataaaaa tgagcaaaat
     3301 caatttagag aacctaacaa cggaacgcag aaaccaagaa acttttggat tagatgagat
     3361 gagtatttct caagccgttc aactaatgaa caaagaagat cagaaagtgg ccgctgcggt
     3421 tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac acgattgagt cctttaaaaa
     3481 aggtggacgt ctgatttata tgggagccgg aacaagtgga agactaggtg tgttagatgc
     3541 tgctgaatgc gtaccgacat tcggtgtaga accggaaatg gtcataggtc taattgctgg
     3601 tggagaagaa gcaatgactg tagctgtcga aggggccgaa gattcttatg agctagggca
     3661 agaagatcta atcaagctga atttgacaaa aaatgatata gttattggta tcgcagctag
     3721 cggtcgtaca ccatatgtga tcggtggatt agattacgca aaatcgatcg gtgctgtcac
     3781 aggaacgatt tcttgcaata aacaagccga aatcagtaaa cacgcggatt tcccaatcga
     3841 agtcgatgca ggtccagaat ttttgacagg ctctactcga ttgaagtcag gaacagcaca
     3901 aaaacttatc ttgaatatga tttctactat cagtatgatt ggtataggta aagtattcaa
     3961 taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat
     4021 catcatgcaa gcgacagaag ctgattacga aacagctgaa aagtactttg aagaagcaga
     4081 tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat agtgataaag aggaagctca
     4141 agcaaaatta gctgaagcaa acggatttgt aaaagggacc ttatgagggg gaaaagacca
     4201 tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca aatttataaa ggggcaggcg
     4261 gcatgggcaa tgtagaaagc attgtccact gcatgactcg tgtgcgtatg aagatcatca
     4321 atgatgatct tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt ggtgtagtag
     4381 aagatgaaca gcttcaagtc atcatcggac ctgggaaagt gaacaaagta gcacagcaaa
     4441 tggttgattt ggcaggcgtg aaattaggag aaaaactttc ttctgtaagt acagctacag
     4501 caaattcatc agcatctgga aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg
     4561 cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac atttctaata
     4621 tctttgtgcc aatgattcct gcatttgtcg gtactgggat cgtggcaggg attgccgcag
     4681 ttttagcaaa cttagtcaca gccggaacac ttgatgcagc aacatggcaa caatatatcg
     4741 atgtgatgaa catcttgaaa aacgggatgt ttagctacct agttatctat acaggtatca
     4801 atgctgcaca agtttttggt gcaacaccaa ctcttggtgg agttatcggg gccgttgtga
     4861 tgctgacagg gatgaatccg gaagcaccac tgaaaaactt atttactggt gaagctcttg
     4921 cagctggaca aggtgggatc ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg
     4981 agaaaaaact gcataaagta gtacctgatg cggtagatat catcgttaca ccaactcttt
     5041 cattgattgc catcggtctg attgaaattt tcttcatcat gcctttagca ggtttcattt
     5101 ctgatggcat ggtcggtggg atcaactggg tacttggcgt tggtggagca tttgccggct
     5161 tcgttctagg cacattattc ttgccgatgg ttatgttcgg actgcatcaa attttgactc
     5221 caatccatgt gcaaatgatt gatgaaactg gtcgtacgct attgcttcca attcttgcga
     5281 tggctggtgc aggacaagtg ggtgcagcat tggcattatg gattcgctgc aaaaaagata
     5341 aagaattaac agaaatgatc aaaggagctt taccagtcgg gatcctagga atcggcgaac
     5401 cattgattta cggagtaaca ttacctctag gacgcccatt tattactgct tgtatcggtg
     5461 gaggtatcgg cggggcagtg atcggaatga tcggaaatgt cggggctatc gctattggac
     5521 cttcaggagc cgctttgatc ccattgatct ctgacggtaa atggttaggc tacatccttg
     5581 gattattggc agcttatgct ggtggcttcg ttgctacatt cttcttcggt attcctaaag
     5641 accgtttaga aaaagaagaa ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa
     5701 aagtagtacc tcaaacttca gctgaaagtg tgactatcct ctcatcagtt gctgacggaa
     5761 cagttgtttc tttagataaa gcaagtgatc cagtttttgc acaaaagatg atgggtgaag
     5821 gctactttgt agaaccagaa aacggacata tctattctcc agttactggg gtagtcagct
     5881 ctgtcttccc aacgaaacac gctattggga tcacaacgcc aaatggttta gaaatcttgc
     5941 ttcatatggg aatcaacaca gttgacttag gcggtacgcc atttgatgtg aaagtaacag
     6001 aagggcagca agtgacacct gatacgcttg tagcagaagc tgacttagaa gcgatcaaag
     6061 cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact
     6121 ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt
     6181 aatatgatag aaacaataga aacgaaagaa gaaactgaaa tctatttttc agcttcttct
     6241 tttttattat ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt
     6301 tccccgtaat tcataatctg ccaagaaagg aaagccaaat gtttgtgcat attgtttggc
     6361 tgttagacaa tactgattat tgaagttttt atttccgcta ccgactacac caagacaaag
     6421 tttatgattg tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga tttctgtatc
     6481 accgttatct aaaccatttc cgccgtctaa ataagtagga acgaaagtaa aaaatggttc
     6541 tgtttctttc gtaaaatcac tattttcatg gatttctttt aaagtaactt caggattgac
     6601 aggatcttct gcatgcattt tttcagcata ttctgctaga tgtttggcaa aggcacgcgt
     6661 atttcctgaa atcgatatat ataaaatgtt catggatcat cctccaattc tttcctttta
     6721 gtatagcatc ttttttaagt tttttggtgg tgtaatttgg aaagggacta ggcgctttgc
     6781 ttgtataatg agaaaaaagc cggtagaatt atatttctat taaagttttt attaattaag
     6841 taaaaataaa aaaggtttgt tatacttttt gataaaagcg gttttatttt ggaggatgct
     6901 tacatggata cagagcaaca gaaaaaagga attggtttaa ttccacttgc tgccttagta
     6961 attggttcag cgattggagg tggcgtattt ggtattatga cggacatctc cggatcggct
     7021 ggcggcccag ctattttcag ttgggtactc gtaggaacag caatgttgat gctctccttg
     7081 tcgatcaata atatcaataa aaaacgtcca gatttaaaag gcgggatctt tagttacgca
     7141 gaagaaggtt ttggccgttt cgctggtttt atcagcggtt ggggttactg gctcacatgc
     7201 tggttaggca acgtagcttt tgcaacattg ttgatgagcg cattaggata tttcttccca
     7261 gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt tttatgggga
     7321 tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca
     7381 ttctgtaaat tagtgccatt atttttattt attgtagtag ctatcagcac gttttcgatc
     7441 caacaattca ttgataactt ttctagcgac attatattga atgcgaatgg acagccgatc
     7501 ccgttttcca gtcaagtatt gagttgcatc atggtgatgc tttgggtatt tgttggtatt
     7561 gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg
     7621 attctaggga tattcggctt gattattatt tacgtcttgg tttccatgct tccatatggg
     7681 acaatgacga tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt
     7741 gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat catttcttta
     7801 gttggtgttt ggctctcatg gacgatctta ccgattgaaa caatgggaaa catggctgaa
     7861 gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc aacttatgct
     7921 atcgtattga cgacgttatg tacgaatgtc tttttacttt cattattatt cactgacagt
     7981 gcttataact ttgcttacac acttggtaca gcagctattt tcttcacgtg gcttttctta
     8041 ggattgtacc aaatgaaact ttcttatcag cggaaagaat gggtccaatt tttagtaggg
     8101 cttttagcaa gtgcctttca agtatgggct atgctagtcg tagcctttaa tgaagtaatg
     8161 cttgttcttg ttcttttctt gccaggcatt tatttttacc tcaaagcacg taaggagcaa
     8221 aacgagcctg tacaacatat ccacttagat aagattttac ttataggtgt gacgatggtc
     8281 ggagtgattg caatggtttt atttgccact gggatgcttc acgtatagaa gtataaaaga
     8341 agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa catactagga aaggagctgt
     8401 gacacaaatg ttgtcacagc tcctttttca gaataaacgg tgtttaagag acagccaatc
     8461 ggcaataagg ctgaacgtct ctttcacagc ctcttttttg atgagtcatc atacattttt
     8521 tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa
     8581 tagcatttga ttagtagaaa gatgagtcaa ttgcttcatt atctcttcgt ttccaatttg
     8641 gttttgcaga ttcaacatac tgatcggatt ccatttgatc cactcccatt tttggatgag
     8701 tgcaataagg ataccagata tgattgacga agcaaaataa aagacgattc ctgctgagat
     8761 ggatgcgccg gaactattga taaagcaagc aatcatcaac actaaactta aaatcagcca
     8821 taaaccgata taactaccca aagtataagt gaacaagctt tggatcagtg tttgaccagt
     8881 agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca acacagtgac
     8941 aataatagct aaaaggtata aataaacaga ataaataact aatgtgatcc atttgcttac
     9001 taatatttct cctcgagaat actttcgata aaggagattt ttcaatgtac catattgtgc
     9061 ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata aagacaatcc aagatgtggc
     9121 actaaaaagc tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt ttttggctag
     9181 aagtgctgtt cctatgagca aaacaactaa tagaacagat aaaacagcag ttgacttttt
     9241 cttgatcaat ttaaataatt cttgttttac aagcaatgtc atttttcttc ttcccccttt
     9301 actgaatcca gaatttccaa tagagaagat tccaaatcat tcgtcagatg ctggacgtct
     9361 gtcacatgga tattttgtgc agacaatagc tggatcacgg ttcctaataa ttcttcgttt
     9421 tcttttaaaa gcttgatttt atcaggatct tccaaagtat atccagcttc tgtcaagatc
     9481 gttcgagcag ctagatcatt tgttgtagag ataacgatga aatcttgcgc tgtttggttc
     9541 aattcagcca ttgtacattg tttgatgatc tttccttgat tgatcaggat cagctcatcg
     9601 accatttttt caagctcgct gagtaagtga ctggagatca agaaagaggt gccctgagta
     9661 gccaagtcgg taatgatttt tcgcacatct cttgttgctt gtggatccaa a
//
LOCUS       DACCSK010000097         9389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCSK010000097 DACCSK010000000
VERSION     DACCSK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.099"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            205..1209
                     /locus_tag="IVP39_002141"
     CDS             205..1209
                     /locus_tag="IVP39_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587205.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1210..2154
                     /locus_tag="IVP39_002142"
     CDS             1210..2154
                     /locus_tag="IVP39_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587206.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2154..3116
                     /locus_tag="IVP39_002143"
     CDS             2154..3116
                     /locus_tag="IVP39_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7587207.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3143..4057
                     /locus_tag="IVP39_002144"
     CDS             3143..4057
                     /locus_tag="IVP39_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7587208.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4083..5864
                     /locus_tag="IVP39_002145"
     CDS             4083..5864
                     /locus_tag="IVP39_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7587209.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6212..6898
                     /locus_tag="IVP39_002146"
     CDS             6212..6898
                     /locus_tag="IVP39_002146"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7587210.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6918..>9389
                     /gene="smc"
                     /locus_tag="IVP39_002147"
     CDS             6918..>9389
                     /gene="smc"
                     /locus_tag="IVP39_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7587211.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPNQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLK"
BASE COUNT         3216 a         1726 c         2016 g         2431 t
ORIGIN      
        1 ctttttgaca cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg
       61 caaagtaaag cgattttcta tataattatt aataattcat tacacaaaga taagaataac
      121 ctttgttata aaacgcttta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt
      181 taggaagcag agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta
      241 catacaggat tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact
      301 ttagacaaaa acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg
      361 gctactacga ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta
      421 tataacgatc tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat
      481 gatatcggaa tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa
      541 gaccagatca aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct
      601 cgtgcactag aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa
      661 tatccccatg aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc
      721 tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa
      781 gcacagattc ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg
      841 atcactcatg acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc
      901 ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat
      961 acacgctctt tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg
     1021 atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca
     1081 cgtattccat ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca
     1141 ccgaaccact tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga
     1201 gaggtgtaga tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc
     1261 agtggattct tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt
     1321 atcgaaaaag gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg
     1381 ggaaaagccg ttgtgggatt agaaaaggtg acagccggac aaatcatgta tgaaggaaaa
     1441 gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc
     1501 ttccaagatt caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa
     1561 cccatccgaa attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg
     1621 ctagatatcg ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc
     1681 ggacaacgtc aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta
     1741 gccgatgaac ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg
     1801 aaacgaatcc agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta
     1861 gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt
     1921 acaagagaag acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct
     1981 attccgcaaa tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa
     2041 caagagtata tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg
     2101 cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga
     2161 aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga
     2221 tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc
     2281 aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg
     2341 tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat
     2401 acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga
     2461 tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt
     2521 atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc
     2581 ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc
     2641 caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc
     2701 gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa
     2761 tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc
     2821 gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt
     2881 tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta
     2941 tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac
     3001 gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga
     3061 cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc
     3121 aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat
     3181 tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt
     3241 ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga
     3301 tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc
     3361 gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca
     3421 attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc
     3481 gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat
     3541 cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt
     3601 cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta
     3661 ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta
     3721 tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat
     3781 gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg
     3841 tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg
     3901 tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt
     3961 acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt
     4021 caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag
     4081 gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat
     4141 gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag
     4201 aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat
     4261 tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc
     4321 aagattcata tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg
     4381 acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa
     4441 aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg
     4501 acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg
     4561 acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa
     4621 tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc
     4681 caagcatgct tcaatcgggt ggtggtattt ggtcatatgc agcaccaaaa catacgttag
     4741 aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt
     4801 ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg
     4861 agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg
     4921 caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag
     4981 atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt
     5041 cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat
     5101 acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag
     5161 ataataatgc agtaggtgag cgtttctatg caggtttgag aagcaatgcg acttcattga
     5221 ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca caagatctag
     5281 acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa
     5341 gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa
     5401 cagcgcaacc attggcagat tactatatcc aacagtggaa agaaatcggt ttggatgtcc
     5461 aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg
     5521 atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg
     5581 gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa
     5641 aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat
     5701 atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta
     5761 atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa
     5821 acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg
     5881 aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc
     5941 aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg
     6001 gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat
     6061 actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt
     6121 acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat
     6181 cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa
     6241 agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc
     6301 atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt
     6361 aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt
     6421 gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa
     6481 atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc
     6541 aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt
     6601 atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa
     6661 agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt
     6721 gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca
     6781 tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg
     6841 aaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa
     6901 gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg
     6961 cagacaagac agtcattgac tttgaaaata gcgtcacagc agtggttgga ccaaatggca
     7021 gtggaaaaag caatattaca gaagctatcc gctgggtatt aggagaacaa tctgctaaaa
     7081 gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc
     7141 tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt
     7201 acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca
     7261 ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagactct ggattaggaa
     7321 aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg
     7381 aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa
     7441 agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca
     7501 tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt
     7561 ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gccgaaatca
     7621 agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat
     7681 taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag
     7741 aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg
     7801 aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac
     7861 aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt
     7921 ttgaaaagct tcaagaaagc ttgttgaaag aagtagcaga aaaagaaaca gaaatccaaa
     7981 aagctgaagc aagtctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga
     8041 aagaattact ggcagaacta agagatcaat atgttgattt gatgcaagag caagcagctg
     8101 taggaaacga actgaagtat ctagaacgtc aatatgtaca agaaacagca aaaagtaagc
     8161 agacgttagc caagcaatcg gaagtagagg catcagtaga ccgattgatc ttacaaaaag
     8221 aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt
     8281 tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta
     8341 aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag
     8401 aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac
     8461 agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca
     8521 cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa
     8581 aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc
     8641 ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag
     8701 ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccaat caaatccaaa
     8761 ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg
     8821 caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga
     8881 atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg
     8941 tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc
     9001 aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac
     9061 aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc
     9121 agctgcaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt
     9181 ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga
     9241 aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt
     9301 taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac
     9361 aagaacaagc agatcttgct gtcttgaaa
//
LOCUS       DACCSK010000098         9055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCSK010000098 DACCSK010000000
VERSION     DACCSK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.100"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            390..599
                     /locus_tag="IVP39_002148"
     CDS             390..599
                     /locus_tag="IVP39_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587212.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            806..1006
                     /locus_tag="IVP39_002149"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IVP39_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IVP39_002150"
     CDS             1493..1672
                     /locus_tag="IVP39_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587213.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IVP39_002151"
     CDS             complement(1718..2323)
                     /locus_tag="IVP39_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7587214.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IVP39_002152"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IVP39_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IVP39_002153"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IVP39_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IVP39_002154"
     CDS             3358..4329
                     /locus_tag="IVP39_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7587215.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IVP39_002155"
     CDS             4469..5782
                     /locus_tag="IVP39_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7587216.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IVP39_002156"
     CDS             5825..6691
                     /locus_tag="IVP39_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7587217.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IVP39_002157"
     CDS             6688..7536
                     /locus_tag="IVP39_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7587218.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IVP39_002158"
     CDS             7550..9022
                     /locus_tag="IVP39_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7587219.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
BASE COUNT         2883 a         1510 c         1842 g         2820 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg tgaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaactttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaacgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgttttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggcaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 gaaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttctaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaat
//
LOCUS       DACCSK010000099         8980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCSK010000099 DACCSK010000000
VERSION     DACCSK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.101"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            141..377
                     /locus_tag="IVP39_002159"
     CDS             141..377
                     /locus_tag="IVP39_002159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587220.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASK"
     gene            396..1649
                     /locus_tag="IVP39_002160"
     CDS             396..1649
                     /locus_tag="IVP39_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7587221.1"
                     /translation="MASDELGDIVTLQIAMLKNSSVRNALKSGVFWDVSEYLKDYKNL
                     SQISEDRIEAASIDGALYGVPIQKDYARAGLVIRKDWLDNLGLDVPTTMDELYEVAKQ
                     FTENDPDGNGVDDTVGFGDRAPSDIRYTSFKLFTSYFGAPNGWAVDDNGKFTPEFDTE
                     EYMKALNFSRDLFENGYLAQDFAVTQKTDQQEQFAQGRTGIYTGMVDIKNLKTMGQGI
                     QDDMELVPVNKISDGEGGGYHVWSEGNGIGGLLAFPKSQVKDEAQLKKLLQFVDDLLE
                     EDVYKLMTGGIEGTHYELNDDGSIEFIDMDLWQQEVQPLSSSRPSEVTFSFKDTDPEK
                     EFSNQLIKENEEFAVMDPSVPLDSPTNNEVGSEIEKIVIDATVQYIMGQLDEDGFKKA
                     VEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            1723..2826
                     /locus_tag="IVP39_002161"
     CDS             1723..2826
                     /locus_tag="IVP39_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7587222.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            2829..4166
                     /locus_tag="IVP39_002162"
     CDS             2829..4166
                     /locus_tag="IVP39_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7587223.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETGRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKTAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSKQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            4153..5031
                     /locus_tag="IVP39_002163"
     CDS             4153..5031
                     /locus_tag="IVP39_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7587224.1"
                     /translation="MQLNDFLGAPSSRRTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVAALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            5028..5951
                     /locus_tag="IVP39_002164"
     CDS             5028..5951
                     /locus_tag="IVP39_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7587225.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKLLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTIKFSEHNNNYETSIKRPEWIHLEKQGVQK"
     gene            5948..6358
                     /locus_tag="IVP39_002165"
     CDS             5948..6358
                     /locus_tag="IVP39_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7587226.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHPTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            6362..7630
                     /locus_tag="IVP39_002166"
     CDS             6362..7630
                     /locus_tag="IVP39_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7587227.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     LAKNITGPYELDPGFPMLTASDKPASTLQCTGYGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNAGRILPNADWCDTKCRPSMCRSGRRIYAGTHWLPIVYNRWSHPISFSTRKDLYDG
                     KLVTSILLICIRNHFASRLPTESFKNCIYW"
     gene            7670..8980
                     /locus_tag="IVP39_002167"
     CDS             7670..8980
                     /locus_tag="IVP39_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7587228.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIRD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT         2951 a         1513 c         1996 g         2520 t
ORIGIN      
        1 ctttatcggt ggtagtctct tcttttccgc agtcacgtgg acagcaaatt ctatattttg
       61 ccatttggaa tggaagaact catatacaga aaaaacagaa gaaaacaaaa atattattaa
      121 aacagtgaag gagaaaattt atgaaaaaag caacgctcgt aaaattaggt cttagttttg
      181 caggaattct agcacttgcc gcatgcggca gcagttcaag taacagttct tcagagacag
      241 aagatggaaa agctaaaatc acttggttaa atattttgca tcatgcttca ccgcctacag
      301 acacgattgt gaacttgatc gaagaaaaaa ccgatacgga actgacattc aactggattc
      361 ctgatgcttc aaaataagaa agattaacaa ctgcattggc ttctgatgaa ctaggagata
      421 tcgttacact tcaaattgcg atgttgaaaa actcttctgt ccgcaatgcg ttaaaatcag
      481 gcgtattctg ggatgtatca gaatatttaa aagactataa aaatttaagt caaatttctg
      541 aagatagaat cgaagctgcg agtatcgacg gtgccttgta cggcgtaccg atacaaaaag
      601 actacgctcg tgctggctta gtcattcgaa aagactggtt ggataatcta ggactagatg
      661 ttccaactac catggatgag ctttatgaag tagctaaaca atttacagaa aacgatcctg
      721 atggaaatgg tgtggacgac acagttggtt ttggcgatcg tgcgcctagt gatattcgtt
      781 ataccagctt taaactgttc acttcttatt ttggagcacc aaatggctgg gctgtagacg
      841 ataatggcaa attcactcca gaatttgata cagaagagta tatgaaagct ttgaattttt
      901 caagagatct atttgagaat ggctatttgg cacaagactt tgcagttacc caaaagactg
      961 atcagcaaga acaatttgca caaggaagaa ctggaatcta cactggaatg gtggatatca
     1021 agaatctgaa gacgatgggt caaggtattc aagatgatat ggaattagtg cctgtaaaca
     1081 agatctcaga tggtgaaggc ggcggttatc atgtttggtc agaaggaaat ggtattggcg
     1141 gcttgctagc atttcccaaa tctcaagtaa aagacgaagc acaattgaag aaattacttc
     1201 aatttgttga tgatctttta gaagaagatg tgtataaatt gatgactggc ggtatcgaag
     1261 gaacgcatta tgaattgaat gatgatggtt ctatcgaatt tatcgatatg gatctttggc
     1321 aacaagaggt gcaaccgtta tcaagctctc gtcctagtga agtgacgttc tctttcaaag
     1381 atacagaccc tgaaaaagaa ttctctaatc aattgatcaa agaaaatgaa gaatttgctg
     1441 taatggatcc ttctgtgcca ttggattcac cgacgaacaa tgaagtagga tcagaaattg
     1501 aaaaaatcgt cattgatgct acggtgcaat acatcatggg acagcttgac gaagatggat
     1561 tcaaaaaagc tgtagaagac tggaaagcac aaggaggcga tcgaatcaca caagagtacg
     1621 aggaagcata caaagcagcg gcacagtaat atagacgatt gggacaaata cttcttggta
     1681 tttgtcccaa atcctatttt aatttttaaa ggagagcaaa taatggaaaa ctggtcaaag
     1741 aaattagcaa aaagtatcat ggaaagaaca cctcgcttat atgaagaaaa gtggtataaa
     1801 ggaaaatggt cttatgacta tggcgtcgta cttaaagggt tccaattact ttgggaacga
     1861 acccaagaaa aaatttattt tgacttcata aaagacaata tcgactattt cgtgcaagaa
     1921 gatggaacga tccgtggcta cagcgttgaa gaatacaata tcgaccatgt aaatacagga
     1981 aagcttttct ttttattgta caaagaaaca ggcgaagaaa aatataaaaa agcagcagaa
     2041 ctcctttccc agcagttggc aaaccatcca cgcacatccg aaggagcttt ttggcataaa
     2101 gaaatttatc cttatcaaat ttggttggat ggcttatata tgggttctcc tttttatgct
     2161 gaatttatcc agaattttag tgaagaaaaa gattaccaag atgttttgag acagtttgaa
     2221 atcagttata aacatctatt cgatccaaaa acaaatttgc tgattcatgc atgggatgaa
     2281 aagaaggtcc aaccatgggc tgatccaaaa acaggtctat cccatcattt ttggagccga
     2341 tcgattggct ggtatttgat ggctgcagca gatacctatg aattacttca tgaagaaaca
     2401 gattgttctt tgctgaaaga aattctagaa aagacactgg ctgcattact tccatatcaa
     2461 gaagaaaaca gcgggacatg gtatcaagta acaacagaaa caaaacggaa aggaaattat
     2521 ctggaagctt ccggcagtag tatgtttgtt tacgcaatgg ctaaaggaat acgtttgggc
     2581 ctgctgaaca aagaagaatg gcttccacaa gtgaagaaat cacatcaagg attacttgat
     2641 gaatttgttc tggaaacaaa agaaggctgg ctgaacttga ataaaaactg tcaagtagca
     2701 gggttaggcg gagcagatca gcgagatggt tcatatgctt actacatcag tgaaccaatt
     2761 atttgtaatg atcaaaaagg cgttggtgca ttcttgcaag cattgattga agtggaggca
     2821 ttataaatat gtatgatatt ttgacatttg gcgcatcaat tgatgaattg aatactgaag
     2881 caatacaaca agccattgat gctgcagcaa gtgacggagg cgggaccgta gttgttccag
     2941 caggtgaatt tctaacagga gctttatttt taaaaagcaa tgtcgaactt catttaagcg
     3001 caggagcggt tctgaaattc tctgacgatc caaaagatta tcctgttgtt cattcacgtt
     3061 gggaaggagt ccatcgaaaa gtttatgctt cctgtatcta tgctcaaaat gtagagaaca
     3121 tctctgtcac tggtttcggt acattagatg gaaatggaaa aaaatggtgg catactttta
     3181 gaaatgaacc agataatctc gcttatcctc gtcctaaatt gatgagtttc cataactgtc
     3241 atcgaattac tgtgaaagat attaaattga tccagtcacc aagttggacg atcaacccga
     3301 ttctttgcag taatgcaacg tttgataact tgacgatctt gaacccagcg gattcaccaa
     3361 atacagacgg aattgatcca gaatcatgca aaaatgtacg tattagtaat tgccatattg
     3421 acgtgggaga tgactgtatt gcaatcaagg cagggactga agatacgtac gaaaggatcg
     3481 cttgcgaaaa tatcacgatc acaaattgca cgatggtcca tggacatggc ggtgtcgtat
     3541 taggaagtga aatgagtggt agtattcgta atattaccat ttcaaattgt atcttccaag
     3601 aaactggtcg aggaatacga ttgaaatcta gacgaggtcg aggcgggatc gttgaagata
     3661 ttcgagtatc caatatagtc atggataatg tgatgtgtcc atttatactg aatctttatt
     3721 atttctgtgg tccgcgaggg aaagaaccgt acgtatggga aaaaacggct tatccgatag
     3781 atgagcggac accagcattc agaaggatcc atttttcgaa tatcacggca aggaatgtcc
     3841 atgcttctgc aggatttatt tatggattag cagaacagtt tatacaagaa atcacatttg
     3901 acaatattga tgtttcaatg gcccaagatg cagttcccgg tgtccctgct atgatgacag
     3961 ggatagagga aatgagcaag caaggatttt acattggctt tgcaggtaag gtttccttta
     4021 accagatgtc gattgaaaat cacgagggac cagcatttct attagaaaat acgaaagaaa
     4081 tcagtattga gaaattccaa tcaaatcaat cagaaaatat ggatgaactg gttgttcgta
     4141 aaaactttgc ttatgcagtt gaatgatttt ttaggtgctc catctagcag acgtacccct
     4201 agtggaaaac gattataccg agaaaaacaa aacggtttta tgcttgaata ctatcaattg
     4261 tacttaaatg aattggaaac agtaccagcc ctctatgctt atccagaaaa agaaggaccg
     4321 tttccgacgg tcctttacct gcattctcac ggcggagatt tttcagtcgg gaaaagtgaa
     4381 ttgatccatg gtgctcctta cttagcttct ccatcttttg cagaagtact gacaggaatg
     4441 ggatatgctg tttggtcgat tgatgcttgg ggatttgaag aacgcggagg tatttctgaa
     4501 agcgagttgt tcaaacaatt tttattaaca ggaaaaacgt tatggggaat gcgtatttat
     4561 gatgtgatca gcttgatcga ttatttggag acaagggaag acacagatac tcaacggctc
     4621 gcaacgattg gtctttctat gggaggctta ttaagctggt gggtggcagc attggattct
     4681 cgtgtcaaag tttgtattga tctagcggca caggtggaca tcgaaacact tttgctacat
     4741 cgccgcctag atcatcatgg gttttattac tatgtgccaa atctattgac agcttataca
     4801 acgttagcaa tccaagaaaa aatcgctccg cgccctcgtc ttagcttagt tggaaaaaac
     4861 gatacgatgt gcttggacga aggcgtactg aagctgagaa aagggctgga caaaaagtat
     4921 caatcaatgg gtagtccaga aaacttttct tctttttctt taacaggcgg tcatatggaa
     4981 acacaagaaa tgagaaacat ttggaaacaa ttcataaggg agcatttatg aggatgaaag
     5041 acaaactgat aattggaaag aatgctatag ataccccgac ggattttgaa actatccaag
     5101 aaggaatcga ttatttggca ggcctgccgg atagccatcc aaagctcttg atagtaaaag
     5161 aaggaagata tgatgaatat atagaaagca gattatccaa ttttaaactt gtgggagcag
     5221 gaacagtagt tatctccggt aatcgttttg caaaacagga gtatcttgac ggtagccagt
     5281 tagggacttt ccgaagtgct acttttttct tagaaggatc gaatgtcgta ttagaaaatc
     5341 ttcacatcga aaatacagca ggtccaggag aaaaggtagg acaggctgtt gctttattca
     5401 attcaggaga tcgcgtatct ttaaaaaact gtcgactatc tggttatcag gataccttat
     5461 gcacgggtcc gttgcctgat ttgcaaaaag atggtactcc tttttgtaca ccggcttctg
     5521 ccaatcctga gtattgtcgt cagacctatg aatattgctg gattgaagga acggtggatt
     5581 tcatatttgg tggggcagat gccgtttttc atcagtgtca tatccatagc ttagccagta
     5641 aaaatcctgg atacatcaca gcagcatcta cgccgaaaaa tcaaaaaaca gggtatcgtt
     5701 ttgaaagctg taccttgact gcagaacctg atactagaaa cgtctattta ggaagaccgt
     5761 ggcgtccgta tgcaaatgtc ttatttgaag cgtgtaagat aggtgggcat gttcacccaa
     5821 ctggttggga taattgggat gacaagaaaa atgaacagac tatcaaattc tctgaacata
     5881 ataacaatta tgaaacatcg atcaaaagac ctgaatggat acatttagaa aagcaggggg
     5941 ttcagaaatg aaaagaaaat gggaactatt gctaggaatg gtcggtggca gtctatcttt
     6001 gattttcttt ggcgggttag cagttacttt aagtaatatg tctgcgtcag agtttaaaaa
     6061 aagttatcag tcgcttgctg tagatcatcc aactttgtct ttagaaaata catttggatt
     6121 attacaggac atgactggtc tgtttgccgt tgtgttgttt atttcacttg cttttttagc
     6181 agtagcattg tttttgactg ctaaaggaaa atacttaaca actgccactg gtttgtattt
     6241 tataacagga ttcatattgt tgattggcac tcagtttatt gcgtttcctt ttgcattttt
     6301 ttatttcgca gctggggcat tcagtttata tcgagtaaga atgagaaaag gagcctgaat
     6361 catgcagact atcaaagtga aaaatcctat tttaagagga ttcaatccag atcctaatat
     6421 attaagagtg aaagagcggt attacattat tgtctcctct tttgaatggc ttccaggaat
     6481 ccgagtatat gagtcgaaaa atttagtcaa ctggtcacat acaacggata ttttgaccac
     6541 tcaagtatct ttgcaaggaa atcctacaaa tggaagtata tgggcgccac agatcagtta
     6601 tgctgatgga ttgttttatc ttgtttacac agatgtcaaa agtgcaggcc gtccgtttaa
     6661 agatgtccac aattatttga tcactgcatc tgagatcaca ggtccttggt ccgaaccaat
     6721 ttatttaaat agtaacggct ttgatccttc tttgttccat gatcttgaaa cgggaaaaaa
     6781 atggcttgtc aatgaactat gggattatcg tctggacaca ccgaataaat ccagcggtat
     6841 cgtattacaa gaatatgatg cagagaagaa gaagcttgat ggtcccatct ataaaatatt
     6901 tgcaggtaca gaattagcga aaacggaggc accacatttg tattttcgta acgggtatta
     6961 ttatttgatg acggcagaag gcggtacagg aagaggacat tcagttacag tttgcctagc
     7021 aaagaatatt actggtcctt acgagcttga tcctggtttt cctatgctga cagctagtga
     7081 caaacctgct tctacattgc aatgtacagg atatgggagc ttagtacaaa ccccgtcagg
     7141 aagatggtac atgacatatc tttgtaccag accattaaac ggtgcggcta ttttgggaag
     7201 agaaaccgct attcaagaag tgtattggac gtccgacgat tggttaagac ttaaagatag
     7261 ccagacaact ccgatggaag aaataatgat tgagacaaat gaagaggtac aacaagaaaa
     7321 agatcatcag tttatggata catttgaagg agagctaaag aagaactgga atgccggacg
     7381 cattttgcca aatgctgatt ggtgtgatac aaaatgtaga ccaagcatgt gcaggagcgg
     7441 taggcggatt tatgctggca ctcattggtt accaatcgtc tacaacaggt ggagccatcc
     7501 aatcagtttt agtacaagaa aggatttata cgatggcaaa cttgttacca gcattctgct
     7561 tatttgtatc cgcaatcatt ttgctagtag gttacccact gaatcgttca aaaactgtat
     7621 ttattggtga agaattgaaa aaaatcaatg aaagataggg agaaacgtat tgcggacaaa
     7681 aaaagggatt atcaatgctt tgaactttaa aacaaacgac actcaacaag atgatacaat
     7741 aatactgcaa aaagcaatcg atcaaggtgc gaaagaacac ttacctgtat ttattccaaa
     7801 aggcatttat ttagtcggag cccttttttt aaaagataag agtcatctta tctttgaaga
     7861 aggtgcagtc ctcaagggga gaacagatat cgaagcattt ccagaaatcg atacacgagt
     7921 agccggcgta gaaatgaaat ggccagcagc gattttgaat gttctctcgg cgaaagatat
     7981 tcttatagaa ggaaaaggaa taatcgacgg tcaaggagac cattggtggg agctgtattg
     8041 gggaaaagat caaaaaagcg ggacaagagc tgaatacgat caaaagggac ttcgttggat
     8101 tgcggattat gctattaaac gtccacgcgc ttgtttactg tatcatgcag aacatgttgt
     8161 tgttcgtgat ttgatttttc agaaatcagg tttttggaac ttgcaaatca cttattctaa
     8221 cgatgtatta gtagaaaaag taatcatccg caacaatgat ggaccaagta cagatgggat
     8281 tgatattgat tcttcaacaa atgtgcgtgt ctatgaatgt gatttggcct gtggtgatga
     8341 ttgtattgct attaagtctg gcagggatgg caatggtgca agagtcaatc gaaaatcatc
     8401 aagaatcgag gttgctagat gtaaaatccg ttcaggatac ggtgtaacta ttggtagcga
     8461 agttagtgct ggagttagtg acgtatatat tcgcgatatc gatttttttc agtcagactg
     8521 tggtattcga atgaaatctt caaaagaaag agggggagtg atcgaaaata tcagggtaga
     8581 aaatctgaat atgattgatg tacaatttcc tttttcttgg ataatggact ggcataatga
     8641 atacaataga aaaaatagca atgaccttgc tcaaatgcca gaaagttggc aagcagtggc
     8701 tgaagaaata cctgaatcaa agcaaatgac gaaagtcaaa gacatacaca tcaaaaacgt
     8761 acaggcatgc ctttctccag gttactcact tccttcccga gcttttgatt tagtagcatt
     8821 tcctgaaaag cctatagaag atgtctgctt ttctcattgc caaataactg caaaagaatt
     8881 cgggcgaatc gaagcagtac aaaacttatg tttcaaaagc tgtgatttat ctatagaaac
     8941 tggcaataca gctgcaaaca atacttttga caatcgttga
//
LOCUS       DACCSK010000100         8979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCSK010000100 DACCSK010000000
VERSION     DACCSK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.102"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(19..339)
                     /locus_tag="IVP39_002168"
     CDS             complement(19..339)
                     /locus_tag="IVP39_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7587229.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(487..2721)
                     /locus_tag="IVP39_002169"
     CDS             complement(487..2721)
                     /locus_tag="IVP39_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7587230.1"
                     /translation="MLCQNCGKNEATIHLYATINGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3200..3466
                     /locus_tag="IVP39_002170"
     CDS             3200..3466
                     /locus_tag="IVP39_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7587231.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3466..5193
                     /gene="ptsP"
                     /locus_tag="IVP39_002171"
     CDS             3466..5193
                     /gene="ptsP"
                     /locus_tag="IVP39_002171"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7587232.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5324..6403
                     /locus_tag="IVP39_002172"
     CDS             5324..6403
                     /locus_tag="IVP39_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7587233.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            6420..7643
                     /locus_tag="IVP39_002173"
     CDS             6420..7643
                     /locus_tag="IVP39_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7587234.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7732..8619
                     /locus_tag="IVP39_002174"
     CDS             7732..8619
                     /locus_tag="IVP39_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7587235.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8705..8935
                     /locus_tag="IVP39_002175"
     CDS             8705..8935
                     /locus_tag="IVP39_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7587236.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
BASE COUNT         2887 a         1591 c         1845 g         2656 t
ORIGIN      
        1 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
       61 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
      121 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
      181 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
      241 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
      301 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
      361 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
      421 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
      481 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
      541 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
      601 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
      661 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
      721 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
      781 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
      841 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
      901 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
      961 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     1021 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     1081 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     1141 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     1201 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     1261 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     1321 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     1381 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     1441 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     1501 atctttttcg gtaatcacag gtgtttcctc ttcagtaagc tgacgttctt tcattttttg
     1561 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     1621 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     1681 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     1741 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     1801 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     1861 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     1921 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     1981 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     2041 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     2101 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     2161 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     2221 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     2281 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     2341 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     2401 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     2461 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     2521 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     2581 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     2641 atcgagttgt gtccgctgac cattgatagt ggcatacaag tgaatcgttg cctcattctt
     2701 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     2761 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     2821 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     2881 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     2941 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     3001 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     3061 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     3121 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     3181 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     3241 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     3301 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     3361 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     3421 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     3481 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     3541 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     3601 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     3661 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     3721 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     3781 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     3841 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     3901 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     3961 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     4021 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     4081 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     4141 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     4201 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     4261 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     4321 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
     4381 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
     4441 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
     4501 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
     4561 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
     4621 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
     4681 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
     4741 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
     4801 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
     4861 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
     4921 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
     4981 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
     5041 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
     5101 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
     5161 gtcgttgaac ttgttcatga atatgtaaaa taaataacgt atcaaaagct ggtgtggctt
     5221 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
     5281 tagacaaaaa tagggcttaa gtttgagtga cagtttgtag gctatgttat actatataag
     5341 tgaaggggga cgtaaggtga aggtattatt atattttgaa agtgaaaaga tgcttgcaaa
     5401 atctggaatc gggcgtgcgc ttgatcatca aaaacgtgct ctgacggaag tcggtatctc
     5461 ttatacacta gatgaaaaag aagattatga catcctgcac atcaatacat acggaatcaa
     5521 tagtcataat atggtcaata aagccagaag agaaggaaaa aaagttgtct atcatgcgca
     5581 ttctacagag gaagatttcc gaaattcctt tatcggatcg aaccagcttt ctcctatcgt
     5641 aaaaaaatat ctggtaggat tgtaccaaaa agcggactat ctgatcacgc caacacctta
     5701 ttcaaaacag ttgttggaaa gctatggtat ccgtgtgccg attcaagcta tttccaatgg
     5761 gatcgactta gagaaatatc accctgatcc aatgaaagaa caaaagttca gagaatattt
     5821 caaactgtct ccggatcaaa aagtaatcat ttgtgtcggt ttgtttttcg aacgtaaagg
     5881 aatcattgat ttcgtagaaa ttgctaagaa gatgcccgaa tacacattta tttggtttgg
     5941 tcatgttccc atgtactcga ttcctcggaa catccggaaa attgtaaaag aagatcatcc
     6001 tgaaaatgtc ctgtttcctg gttacattcg tggagagatc atagaaggtg cgtattcagg
     6061 tgcagatcta ttctttttcc cttcttatga agagacagaa gggatcgttg ttttagaagc
     6121 tttagcaagc aaacaaaacg tacttgttcg agatattcca gtgtataatg gctggttaga
     6181 agatagcaag aactgctata tggggaagac aaacgaagaa tttatccgac tgatccaaca
     6241 gattaccaat caagaactac cgagtacgac acaagccggt tatcaaacag caaaagagcg
     6301 aagtatcaat aaaatcggtg aagaacttaa aaatgtatac gaaagtgtac tgaatgaaaa
     6361 agtctctagc aaattaaaaa aagtaaatca ggtcaaggac tagtttggag gcgaaaaaca
     6421 tgaaaatcgg cttttttacc gatacctact ttccgcaagt cagcggagtt gctacatcga
     6481 tcaaaacatt aaaacaagaa ttagaaagac atgggcatga agtctatatt tttacaacga
     6541 ccgatcctaa tgcggataag ttggaaaaag atgtgattcg aatgccaagt gtaccattta
     6601 tttctttcaa ggatcgaaga gttgttgtta gaggcatgtg gtatgcttat ttggttgcca
     6661 aagaactaga actagatctt attcataccc atacagaatt cggagcaggg atacttggga
     6721 agatggtagc taagaagttg aaaatacctc tgatccatac ttaccatacg atgtatgaag
     6781 actaccttca ttatatcggt aaaggaaaag tgatcaggca atcccatgta aaatacttgt
     6841 ctagattatt tgcaaatcat acgacaggtg tagtttgtcc aagtgaacgt gttattgata
     6901 cattacgcag ctatggtgta atagcaccgc ttcgcgtgat tcctactggt atcgacattg
     6961 aaaaattcaa acgttctgat atcactcagc aggatatcag caaattaagg gagtcactag
     7021 gtcttcagga aaaccatttg atgattcttt ctttaagcag gatttcttat gagaaaaata
     7081 tccaagcctt gattaatggg tttcctcagg tcatctccgc ttatcctaac gccagattag
     7141 ttatcgtagg aaaaggacct tatgtagaag agttagagga attgatctca gaactgcagt
     7201 tagaagaatt tgtgcagttc acaggagaag tggacaacaa tgatgtagca ctttattata
     7261 aagcggcaga ttattttgtc agtgcttcta cttcagaaac acaagggctt acttatacag
     7321 aagcgatggc agccggaaca ccttgtgtcg tggaaggaaa tgcttatttg aaccgattgt
     7381 tcgatcatcc ttcattaggg aaaacttttg aaaccgacga agactttgca ccagcactta
     7441 ttgattatat ttccagtgaa gttcctaaag atccaaaagt tttagaagct aaactttatg
     7501 atatttcatc tacacatttc ggcgaagcta tgatcgaatt ttatcaagac atggttgttt
     7561 atcatgaaga agagatgcga agaaaagaag aagaagactc gattgaacga atcaaaataa
     7621 aattaaattc atttaaaaga taaaattcaa acatttttta tgaaaaaatc cttgtttcta
     7681 gcgtatacta gcatagaagc aaggattttt gatttttagg aggtcgaaaa aatgaaactt
     7741 ggttttatcg gaacaggcgt catgggaagt gctgtcgcac gccatctact agaagcagga
     7801 cacgaagtag ctgtctataa tcggacaaaa gcaaaagctg atcctttagt aacagaagga
     7861 gctatctggg cagatacacc aaaagcggta gcagaacaaa gtaatattct ctttacaatg
     7921 gtaggttacc ccaaagatgt agaagaaatc tactatggac agtcggggat tttttctgca
     7981 gatatctcag gacatatttt agttgatttg actacaagta caccaagcct ggcagaaaaa
     8041 attgcaaaaa cagcaaaaga aaaaggcgca gatgcgttag atgcaccggt atccggtggt
     8101 gacttaggag caaagaatgg tacactgacg atcatggtcg gtggagaaga agccgtttat
     8161 gatcaagtac tgcctttatt caaagaattt ggtacgacat ttacattgca tggttctgct
     8221 gggaaaggtc agcatacaaa aatggctaat cagatcatga ttgcaggtac gatgacagga
     8281 atgacggaaa tgcttgtcta tgcacaaaaa aacggacttg atttaaagaa agtgattgag
     8341 acgctttcag gtggaagtgc agctaactgg tctttaagca attattctcc tcgtatttta
     8401 aaagaagatt acacacctgg tttttttgtc aaacatttta tcaaagattt aaagattgca
     8461 ttagaagaag cagaaaaaat ggatcttgta ttgccagcaa ctacccaagc attgaaacta
     8521 tatgaagagt tggcagataa aggttttgag aatgacggaa cccaagcttt gatcaaactt
     8581 tggtggcctg aaggaaaaat accagaaaaa aaatcataaa aaacgagttt ttttagagaa
     8641 aatcaatgca agtaggctat gcttttgata caatataaca gaaataagaa aaggaggacc
     8701 tccgatgaca cattcacaat tagtcgcaat cataaaacga ctggaggcta tggtcgagtc
     8761 agcagataat gaactgcaag ttcgccgttt tgaaaaagaa ggcgtagaga agtgtacagt
     8821 aacttatgat aaagcgaccg atacatttga attaactgaa acagatactc atcagcaata
     8881 cgaatttgat aatatcgata tcgtagcgat ggaaatctat gacttaatcc aataaatttt
     8941 aggataattg acatttgaag ttgaagatgc ctaaaagaa
//
LOCUS       DACCSK010000101         8940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCSK010000101 DACCSK010000000
VERSION     DACCSK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.103"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            269..664
                     /locus_tag="IVP39_002176"
     CDS             269..664
                     /locus_tag="IVP39_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587237.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            661..915
                     /locus_tag="IVP39_002177"
     CDS             661..915
                     /locus_tag="IVP39_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587238.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            958..1353
                     /locus_tag="IVP39_002178"
     CDS             958..1353
                     /locus_tag="IVP39_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587239.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            1353..2408
                     /locus_tag="IVP39_002179"
     CDS             1353..2408
                     /locus_tag="IVP39_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP7587240.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            2405..2611
                     /locus_tag="IVP39_002180"
     CDS             2405..2611
                     /locus_tag="IVP39_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587241.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            2623..2946
                     /locus_tag="IVP39_002181"
     CDS             2623..2946
                     /locus_tag="IVP39_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587242.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            2939..3205
                     /locus_tag="IVP39_002182"
     CDS             2939..3205
                     /locus_tag="IVP39_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587243.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(3368..5713)
                     /locus_tag="IVP39_002183"
     CDS             complement(3368..5713)
                     /locus_tag="IVP39_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7587244.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            5992..6894
                     /locus_tag="IVP39_002184"
     CDS             5992..6894
                     /locus_tag="IVP39_002184"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7587245.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            7105..8880
                     /locus_tag="IVP39_002185"
     CDS             7105..8880
                     /locus_tag="IVP39_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7587246.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         2937 a         1594 c         1799 g         2610 t
ORIGIN      
        1 tttgtcataa cccctttttt ttgtgacttt aaaaaaagag aaaatggcgg aaacaaacct
       61 tacgtccatc gtatacgaca caatctgtgc gtagatgcta cattttgttc actactatca
      121 aaattacttt aaaatgaaaa cgtgataaaa aagaaagtag aaaagtagaa aagtaactct
      181 aaaaaatcca aaaaaatcca ggctcgattg cgtacttatc ttaaaggaga aacaatccat
      241 catacataac ccgaaaggag acttctattt gcctaagaaa agacgaaaga ccatagtcat
      301 cattttgacc attcttgcta taggaatcgg aggaaaattt tacatggaca aaagagaagc
      361 acaaagacag aaagatttac tcgcagtaga aaaacaatcc gtcaaagttc tgaaaaatac
      421 ctttgcagat atcagggaag tgaaggtaga agaatttaaa aaaaaccctg ttactggatg
      481 ttatggtgca cttgtgacaa tgacaaataa tgaagggaaa tctgtttatt ttgattattt
      541 tttttcaaaa caaatgaatg aaattagcag ttatggtgta gaaaatgaag aaatacaaaa
      601 cgaaggggtt actactccta aaataagagt tatttattct aatggagagg cggaggaagt
      661 gtgacagtaa aagacgaaga gtatagatat ataagtgaac atgtttattg gctagatcca
      721 aaacatcctc aatatagtaa aagttacaaa gaaggcgcca ttaaaaactt tggagaaaca
      781 aaattccaaa tcctaaaaat caaagactca ttggatggca tgcaggcgat ggctgcagcg
      841 ccaatcgtcc atagcaaatt ggagaaaaat ttcaaaaata aaaaaattcc tacaaacttt
      901 cgcgtactta aataatgaag gtatcaatac ttacataaaa agaaaggagg cacgttcttg
      961 tctcggaaaa taagaaaact cagcttagcc attttattaa tcgtcatatt aggaatcgga
     1021 gggaaagtct atatggacaa aagagaagca caaagacaga aagatttact cgcagtagaa
     1081 aaacagtcag tcaaagtttt gaaaaatact tttgcggata tcaaggaagt gaagattgaa
     1141 aaatctgctc gaaatgaaat gacgggagca tatcgtatgt ttgtgacaat gataaataat
     1201 cacgatgagt ctgtttattt tacgtttgga ttttggaaag aacgaaatga attaggagat
     1261 tatggcgtag aaaataaaag tatacaaaaa gaggggatta cagtaaataa aatcagagta
     1321 attttttcta ataataagga ggaatatatt taatgatggc agacaaacaa tacagtgtta
     1381 ttagtgaggc tgtttactgg ctagatccta aacacagaga ctatgatcat acttataaag
     1441 aaaattctat taaaaaaatt gatagttata attttaaaat cctaaaaatc aaagactcat
     1501 tggacggcat gcaggcgatg ggtgtagcgc caatcaagaa cggtaaagtg gatctttctc
     1561 aagtagtgat tgcttttgca ggaacgaact taagtgattg gaaagatttg gaaacggatg
     1621 caaggagtat catgaatctc tatgggaata gaccaggagc aagtttttct gaaaaagcaa
     1681 caacaaacaa gattttatcc gttagtggat tgaaaacagc ggggcaaatc aattcagcga
     1741 ttgcatttgc taatgaagta aaaaaggaat atggagaagc tgctatttct actaccggcc
     1801 attcattagg tggattctta gcattatatg tagcagcaga gaaacaatgg aaaaatgtcg
     1861 gcttaaacgg gccggacccg tatgatctct tatcaccaga agcaaaaaaa tgggtggaag
     1921 aaaatcccgg tatgctcacc aattaccgaa atcacgccga ttttatcggt aattttggtg
     1981 gtaatggcac aggtgctgaa gttcggatca gtatggaaac agcattcttg aatctgcttg
     2041 cttcccacag tttaacctct tggaagtttg atgaaaatgg aaagctgatt attcctgaaa
     2101 atgaaaataa tgttaaagct cgaaaacaac agaaagaaaa ccaagcgacc cagttgtttt
     2161 cctatcagtt gcaagaactg aacaacatac gtaaaaaact acgattaagt ggcggccggc
     2221 tgacagttaa tgaacaaatt tacttagatg acacacaagc taggttagta gtggaaaagt
     2281 tatcagcaaa aatgaaaagc gctatggaag aagttatccg catcaatcaa agaggaattt
     2341 ttgagctaga gaaaaaatgg catggcaaga aggcttgcgg caagcacgtg ttgtcgcccc
     2401 ttcgttgact gaacaggaaa tcatagaagc attagctagt gttgtcgcca caaaagagaa
     2461 cttagtcgat tctgcgaaag aagtctatct gctgcgaatc aataaggcaa gacgtatggg
     2521 ggaggccttt gatacattgg tgaaggagat ccaagacaaa atcaatgaaa tcgtgacacg
     2581 agatcgagaa ttagcacagc aattcaatta aaggagaagt agatgaaact agaagaattg
     2641 gaaagagatt atcgaagtga acgaagacaa ttagaagctc aagaagatga attgatccac
     2701 tttaagaatc aaggattacg attgatggag caagaggagc agcgactgca tcatgtatta
     2761 aagaacttag ctttagataa tgagcctctg tttcaagcaa gacgaagtta ttcgaattat
     2821 caggagcatt atcaacaagc cctacaagta gaacgacgaa aactctatca gcagcaagac
     2881 gaactagaac gttcctatcg tcaaaaaatc agacaaataa ctatggagaa atcatcttat
     2941 gaatgaattt ttaatgcaat tactaaaaga attagagaaa aatctacatc gatcaaatgt
     3001 acgttcagtt ggtatgggga tatctcagca aatttgggca gaaaaacgat tgacagatca
     3061 aaaccaccaa atgactgcag aagaagctgc tcaagaattt tctaaccgac atgtacactt
     3121 gaaagaatca gtaaaagggc aatttgcaga aaaactaacc gaattattcc aaagacaaga
     3181 aaagaaatta aaacagtttc gttagtaaag ttatatagaa aaattttgtg aaaatggctt
     3241 ttcacatttt tacataaatt tgtcttaata tcgtagatgc aagcttttga acaccattta
     3301 ttcggaaaaa gggagctgtg acaagatttt tgtcgcagct ccctttaatt ctgattaatc
     3361 tattagatca atcatcttta tcgtctgatt ctttggtctc tggattcgct ctagcataag
     3421 ctgaagcggt gttccagtaa tctgtatagt tttcatcagt accgccaacg ccgaatttaa
     3481 aggcgctttt cttcggacca ttttttgccc aaagtgattc cactgttttt cctggcgttt
     3541 ggatagattt tttattgaca gtaacttttc cagctgtttg tcctgtgaac ccagatactt
     3601 ttttcttcat cacatcagat gataagtcaa atttctcatc agtgccgaaa atctcagggt
     3661 ttgcttggta agcttgattg attagattgg caagataatt agccgaacgt atccttgctt
     3721 ggctgtccat tccagtattg tcatcgtgtc ctgcccagct actgatagtg atagacggag
     3781 tagagacgat cagccatgaa tctctgtatt cgttggtaga accagttttt ccgacccaat
     3841 ctgcttttcc tagatttccg tttaagcttg aaatgacatc tttgaatggt gtagtgattt
     3901 tttcattgat cacactgcgc atcatgtcat tcatgatcga ggccgttgct tcggaataaa
     3961 tacggacaga attactttca tgtttataaa tcacattacc tgcattatct gtgatggaat
     4021 caatgatata gccttcttcg tagacgccac cattagctaa ggcctggaat ccatttgtct
     4081 gttgaagcgt cgttacatct actgtcccta aaggagcaga ttcaacgccc caattatcat
     4141 ttgctgggta gttcatcttc gccaagtagt gctcgtaagc atattgtttc gatccacccg
     4201 aattcaatac gtcttggtat aagtgataag ctgggatatt gttggaccat tccagtgact
     4261 cacggacggt ttggaaggtg ttcgaccctt cattggtcgc attgacgatt ttctcgccag
     4321 cattttctcc ttcttgccaa gtcgttgcat aatctgatac acgggattct gatccgacaa
     4381 gtcccatatc gattgcagga ccgtaaacaa gaaccggttt gatggatgaa ccagcttgac
     4441 gttctgtatc aaaggcgtgg ttattttgat tctggctgta attccggcca cccacgaatc
     4501 catagatacg tccagtccta ttgtccatca atacatttcc agtctccact tggctaccag
     4561 caccatcatc taacatatag ccgtaatttt ctacgccggt ttgcaatgca gcatagatat
     4621 ctttatcgat cgtcgaatgg atcgtatatc ctttgttttg gatcgtttgc tgtgcttttt
     4681 gataataagc ttcactgacc gatgaatctg acatatcagc attatctttt tctgccaatt
     4741 gttgtgcaat aatccttgtt gcttcgttca tcacggtata atacaagaat tctctgtcag
     4801 tctgttccgc gatttgttgt gggagaaagt cttttgtaag atcataggct ttcgcttctt
     4861 cgtattgttc tttcgtaatt tgtccttcac ggtacatact gaataaaaca aaatcttttc
     4921 tggcaagacc ggcagaaaga tcttctttta gcgcgcctgt attcgtgtag ggactatacg
     4981 tgatagggct ttgaggcaaa ccagcgatat aagcagcttg tggcagagtc acatcttttg
     5041 catctacacc aaagattcct cttgcagctt cctggactcc agcaatattt tgccccttat
     5101 tattccgacc aaatggcgag acattcaaat acgtagcaat gatctcatct tttgaaaagt
     5161 atttttctac ttgtgcagaa agaacgatct cgtttgcttt acgtttgaat gttgtttcat
     5221 cggttaggat ctgctgtttg actaattgct gtgtcaaagt ggatcctcca gacgagccga
     5281 tgccagtagc ttctgaaact aatgctcgta aaacagcttt agggacataa ccttggtgtt
     5341 tatcgaagta ttcatcttct gtactgataa tcgcggtttt caataaaggc gaaatcttat
     5401 cagatgaaat agtcgtccgc atcagatctg tctggatttt tgatatctct gtattatctg
     5461 cataaacaat ctttgacgtt tccgtaatgt ttcctagttc tgtctgcagt tcttttttag
     5521 tcggcaattg tgtgtcttcg acaagatagg caaagtagcc agcacctatt ccgataccta
     5581 gagctgcgaa caacaaaaga agaacagtaa taaaaacagt caatgattga aacactctaa
     5641 aaataacctt aggaacaaac catttttctt tttttcttct attctgtttt tttcgagtat
     5701 ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt atagcaaata
     5761 ataagacaaa aaaacagaca aggctgtttt tgacaggaat ttctttttcg aatgagataa
     5821 aacgtgagga aatgatcttt tttatgaaag agttcttaag cttttcttta gagaaagttt
     5881 cagttactat ggagaacagg gcaaagaaaa acaaaccatt ttctttgtac atcatcttac
     5941 caatatatag gaattgttcg aaacaattgc ctttcaagga ggaatccatc catgttatta
     6001 ggctcacacg tcagcatgag cggaaagaaa atgctgttag gcgcggcaga agaagcagcc
     6061 agttacggat cttcgacctt tatgatctat acaggcgctc ctcaaaatac tagaagaaaa
     6121 gctatcgaag agatgaatat tgatgccgga aaagcattta tggcagaaaa cggactaaca
     6181 aatatcgtcg ttcatgcgcc ctatatcatt aatttgggaa atacgatcaa accagaaatg
     6241 tttccatttg ctgtacaatt tctgagagaa gagattgttc gtgcggaagc tttaggtgct
     6301 actcaaatca cccttcaccc aggcgctcat gtaggagcag gggcagaagg tgggattgct
     6361 agaattattg agggtttgaa tgaagttttg acaaaagaac agacggcaca gatcgctttg
     6421 gagacgatgg cagggaaagg tacggaaatc ggtcgaacat ttgaggaact agcgcaaatc
     6481 atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgatacatg tcatacgaat
     6541 gatgccggat ataatataaa agaagatttc tcaggtgtga tggaagagtt tgataaggtc
     6601 atcggattag atcggcttaa agtactccat atcaacgatt ctaaaaatcc acaaggttct
     6661 cataaagatc ggcatgccaa tatcggtttt ggaacaattg gatttgacac attgaacaaa
     6721 atcgtccatg atccaatgtt tgacaacatt tcgaaaatct tagaaactcc atatgtcggt
     6781 aaagataaaa agaaagctta cgcaccatat ggaaaagaaa ttgccatgtt caaagcgcaa
     6841 gcattcgatc ccgaagtgtt tcctgagtta ttagcagaag aagtcacact ttaagttttc
     6901 tatctgacag caacaaaaaa ggattgggca gtctgccttg tccttttttg tttaagaaac
     6961 aaaggagtgc tttttgcagt cgttttcgcc atgctccttt cgccccctct ttttaaattc
     7021 agacaaacag ggtatactga tagaaaagta aaaaacgaaa agattcacaa gcaagtagca
     7081 aaacgtgatt gaaaggacga aaagatgctg aacactttaa aacccttgat caaccaacta
     7141 aaaaagaaac atacatactt tattcttggc atagccgtat tatttacaat tattcggttg
     7201 tatttatata gtaaagcaat ctatggaaca gatatgtcgg caacctatga tgatcagctg
     7261 ctgattcact atgctcaaaa tattttggat ggtaaatggc taggggagta cacgactacg
     7321 acactttcaa aagggatctc ctacagcttg tttttagtac ttgcacataa actgcatctt
     7381 tcttatgcag ttttgttcgg catgttcagt attcttgcca gcatattggt tgcactagcg
     7441 tttaaaccga tcgtgaagaa caagtatctt cttttaagta tctattttta ttttttgttt
     7501 tctccgatta gttttacaca tgagtattcc actcgaacgt atcgtaatgc tctcgtgatc
     7561 ccggctgttg agatcattat cgcttgttta ctagctattt attaccgtaa atatagttcc
     7621 cgcagaaaat tgctgccgtg gtttttaggg ttagctgttt cttttccttt cttttggttc
     7681 attcgcgaag attccatctg gcttcttcct tttactgtag tcgcgttagg catcagtttc
     7741 gttcagatct tgctaggaca agacttttct ttcaaagaag caaagaagtt aaaagaacga
     7801 ccatttctcc tttcgaaaca agaatggctg aaactttcat tgtttttact ccctctagtc
     7861 ggtttctcca tcgtctatca gtggatcgaa tataaaaacg agacaacata tggtattgcc
     7921 accatcaatg accgttcgag tggtgaattt ggacgtttga tgaaacagct gatccgaata
     7981 gatgacgaaa cgaacttaaa taaagaaaac agtgagatat gggtctcgca tgctgcatta
     8041 aataaagcat tagacgcatc tcctacattt catactgttt ctactcgtat tgacgaattg
     8101 tatacgactc atgcgtggac ccaaggcgga aaagtaaaag aattaccagg agatatcatt
     8161 ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa tgcaaaagaa
     8221 accaatgatt tttggaaaaa agtcaatcaa gaattagaag aagcctatca aaagggaact
     8281 atcaaaaaga aaaaagaatt ctatctgatg ggatcagggg atggaaagac gatgaatgat
     8341 ctgccaatcg tatttgcttt tctgaaatca ggatatgcct atgcatggcg atacaaagat
     8401 ttccatcaag gagggaatta tagtttcggg acgcaagaac aagtcgaaca ggcccaagaa
     8461 ttattgaatc ttccactaat ggataattgg atcgatagca gtgatccgag aacacctgat
     8521 ttccagttga caaaaacagc aaaagtcgcg aatgtcatga taaaaattta ccaagttctg
     8581 gccattcccg tgctgattgc ttcaatagca gggatattac ttatgatact aggaaccgta
     8641 tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtagtcac cggattaata
     8701 ctgacacaat tggtttttct tttcggtgtc tcgtggtttt gttcttatgc gccagaaaaa
     8761 aaggattatt ttttaagtgt ctacactggc gcaggcgtgc cattaacaca attcatcgta
     8821 gtaacaagca tgctagtttt cagtaaattg aatctgaaaa aagctttatc ttctaaataa
     8881 aaagggaact gtgacaaaag tcttgtcgca gttccttttt ccgaataaac ggtgttcaaa
//
LOCUS       DACCSK010000102         8622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCSK010000102 DACCSK010000000
VERSION     DACCSK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.104"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..425
                     /locus_tag="IVP39_002186"
     CDS             <1..425
                     /locus_tag="IVP39_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7587247.1"
                     /translation="DKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFYYQE
                     ILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKIENL
                     HTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(514..714)
                     /locus_tag="IVP39_002187"
     CDS             complement(514..714)
                     /locus_tag="IVP39_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7587248.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(1166..1486)
                     /locus_tag="IVP39_002188"
     CDS             complement(1166..1486)
                     /locus_tag="IVP39_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587249.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFASTHTQKYDGHHPV
                     IYEV"
     gene            complement(1989..3107)
                     /locus_tag="IVP39_002189"
     CDS             complement(1989..3107)
                     /locus_tag="IVP39_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587250.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPEIEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            3578..4885
                     /locus_tag="IVP39_002190"
     CDS             3578..4885
                     /locus_tag="IVP39_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7587251.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTVENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            5004..6203
                     /locus_tag="IVP39_002191"
     CDS             5004..6203
                     /locus_tag="IVP39_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587252.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTISFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(6230..7498)
                     /locus_tag="IVP39_002192"
     CDS             complement(6230..7498)
                     /locus_tag="IVP39_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7587253.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(7661..8308)
                     /locus_tag="IVP39_002193"
     CDS             complement(7661..8308)
                     /locus_tag="IVP39_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7587254.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVLALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         2626 a         1774 c         1517 g         2705 t
ORIGIN      
        1 aggacaagct tgtttatgac aaatggacca aatggcgcaa ttttggttgg gcgtatctca
       61 ccgaaagtga agtggtggat cgtctgcttt cgatctctga cgaattacga atcgcctatt
      121 tttactatca agaaattctt caagcgtttc acgacaaaga ggcggacact ttctttaaac
      181 ttgtaagaac gatgcccaat tccgttccaa aagaactgca ttctataaaa aaagctttta
      241 taaactatga atccggcatt cgtttagccc tcgaattgcc ttattcaaat gccaaaatag
      301 aaaatctgca tacccacatc aaagcattga aacgagtcgc ttatggcttt cgaagctttc
      361 ggaaaatgaa gaccagaatt ttcttactaa ataatctgat tacctatgaa tcaaaaaaca
      421 tctaaagtag aaaaggaatt ctaccttaga tgttcttatc tcactctatc agtcctattt
      481 gacaaagagc ctttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
      541 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
      601 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
      661 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
      721 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
      781 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
      841 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
      901 gattacataa agttaaaaac cgaacttttt ttaaaataaa cgacatattg ttcagttggt
      961 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
     1021 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
     1081 tatttctgat agcctatgac agtgaccaga agaacaaggc aaaccaatac gatcaacgag
     1141 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
     1201 atttctgagt atgagtagaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
     1261 tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
     1321 cttctgtttc aggaatcgca gaatcaagat ctgttgtctg tacataaaca tttttgtaca
     1381 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
     1441 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acctccttat
     1501 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
     1561 ataaatgatg ttttttgtat cataatcgtc aaaaagagtt cgtgaccatg tcacaacctc
     1621 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
     1681 ttgagaaata atttttgtaa atctcttctt gtttcttgaa gctgactact tctatcacaa
     1741 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
     1801 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
     1861 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggcaaa
     1921 atatgtgaaa ctatttttcc gtttgtttct ctaacactca ggtcaaaaca ctgatttcac
     1981 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
     2041 tccaaaataa atactttccc gccattaatg atcacatcat ctgctaatgc attcaacact
     2101 tgacgccgat catattctat ttcaggatct tcaccgaaac catcagctaa gtttgacgta
     2161 gcgataaata gatgcgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
     2221 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
     2281 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
     2341 gaaatcgcaa cgtctgcaca atagtaagga tttttggcga tttttttgaa catcgtctga
     2401 ttttctggta gagcgaataa ataaatcggc cattgttctg gattttcgaa agaatccttc
     2461 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
     2521 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
     2581 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggcgcccct
     2641 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     2701 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     2761 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     2821 taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     2881 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     2941 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ttgatctttt
     3001 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacaaatgg tcctttagcg
     3061 ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa cgtacccctc
     3121 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     3181 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     3241 agttatcttc ataatttcct caattatata gccccagtct tcaactattc ttcatgaaac
     3301 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     3361 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     3421 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat aacgtgcgtt
     3481 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     3541 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     3601 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     3661 gcaagctgaa gatacattca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     3721 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     3781 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactat catgggatga
     3841 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     3901 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     3961 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     4021 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     4081 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctgtagaaaa
     4141 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     4201 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     4261 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     4321 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     4381 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     4441 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     4501 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agcctaaaaa ccattgatgt
     4561 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     4621 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     4681 cactgacaac gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     4741 caccttggca gacgataaat taggttattt agaagataac aatgcgccat cgacagagat
     4801 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     4861 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     4921 atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg
     4981 tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct
     5041 atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt
     5101 tcttttacgg cagtacatgg acatttacgg taatcttaac ttattttgtc gtgttggaaa
     5161 ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt
     5221 cttatttcac agaaaaacga aggctgctga atggtctttt attcaatgtt ttcctcatca
     5281 gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttc cttggcggac
     5341 tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga
     5401 ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta
     5461 atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct
     5521 tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga
     5581 tctacctatt tatcgtattc tacaactttt tgactatctc attcttgtat cagttcaacc
     5641 ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tgggttgatc aatggagaaa
     5701 cagtttctcc tcttctagcc aagcgaatca ataaggcaat tgctttttat cgggctcaaa
     5761 gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa
     5821 aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag
     5881 acattcttgt cgaaaccaat tcgacaacca ctttggagaa catgctctat tcaaaggaaa
     5941 tcatggatca gcaaatgaaa gggccatacc gcgcgatctt tagttccaac aattaccaca
     6001 tttttagagc cggtctttat gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa
     6061 aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacatcgcc atcttgatga
     6121 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
     6181 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
     6241 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
     6301 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
     6361 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caagcaatcg
     6421 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
     6481 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
     6541 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
     6601 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
     6661 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
     6721 tatttcgcca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg
     6781 aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
     6841 acagcaacat actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat
     6901 agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
     6961 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
     7021 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgctt
     7081 tatccaatcg gaacaaacgt gctgtattat ctgctaacgc tccccccatt tggactgctg
     7141 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
     7201 gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
     7261 tgtttccacg actagaaaga ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
     7321 taactggtag tagataaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
     7381 ggctatttaa gaaaaaagca gataagctcc ccatgaatat accgagaatc gctgtgatca
     7441 ccagccactt cagcatatac actaaaatgc gcactggact cggtaattgt ttttccataa
     7501 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
     7561 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
     7621 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
     7681 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
     7741 catgacaaat ataatttccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
     7801 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
     7861 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
     7921 ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat
     7981 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
     8041 taggctgcat aaacggctgg acgatttgct tcagctcctc caatgctctt tctgcttgcg
     8101 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgatttct ggcagttcgt
     8161 gttctaatat gtctcgaatc ttttcagctg ctagtgccag aactgtttgt accgtttttg
     8221 gatcattgac agatgagtac gcatttaata actgttctac ttctcttttt ataaaaaaat
     8281 attggtatgg ttttattgtt tgttccatga ttttaaagtc ctttcttcta accaaagtgc
     8341 atactttgta gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga tcccggactc
     8401 ttttaaataa gatacgtttt gcatcttatc atcaccccct ttaatgctta ttcttctatg
     8461 tacatgattc attcgataaa taaaagtaaa ccgtatcttg aacagatagt caactgcaaa
     8521 cagaaaaaag gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat gaatggtgtt
     8581 caaaagcttg catctgcggt aataagccga aacttcacaa aa
//
LOCUS       DACCSK010000103         8574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCSK010000103 DACCSK010000000
VERSION     DACCSK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.105"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..1218)
                     /locus_tag="IVP39_002194"
     CDS             complement(<1..1218)
                     /locus_tag="IVP39_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7587255.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAP"
     gene            complement(1325..2185)
                     /locus_tag="IVP39_002195"
     CDS             complement(1325..2185)
                     /locus_tag="IVP39_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7587256.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(2244..2874)
                     /locus_tag="IVP39_002196"
                     /pseudo
     CDS             complement(2244..2874)
                     /locus_tag="IVP39_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            3083..3802
                     /locus_tag="IVP39_002197"
     CDS             3083..3802
                     /locus_tag="IVP39_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7587257.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            3828..6419
                     /gene="mprF"
                     /locus_tag="IVP39_002198"
     CDS             3828..6419
                     /gene="mprF"
                     /locus_tag="IVP39_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7587258.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(6607..7794)
                     /gene="tuf"
                     /locus_tag="IVP39_002199"
     CDS             complement(6607..7794)
                     /gene="tuf"
                     /locus_tag="IVP39_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7587259.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            8162..8518
                     /locus_tag="IVP39_002200"
     CDS             8162..8518
                     /locus_tag="IVP39_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7587260.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         2525 a         1737 c         1535 g         2777 t
ORIGIN      
        1 aggcgctcct acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc
       61 agaagaaaat gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct
      121 catagcccgc atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc
      181 tgggatggct ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc
      241 ttcagtaatg gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat
      301 tgaatacatg tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa
      361 tgcaatactt attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga
      421 tgcaataaca gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat
      481 gatccccaaa ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac
      541 aagttcagaa cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt
      601 atctgcacct actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga
      661 aagcaattca gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc
      721 atttgcttct gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc
      781 gatttttacc aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc
      841 ccggatcact agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc
      901 atgtatttct tgataaattc caataacgat attgacgaca ataattgcta aatacgccat
      961 gttggaatac gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa
     1021 taacgtcaat atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta
     1081 gtcattttgc tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat
     1141 tttctttttc tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg
     1201 ttccaagatt aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt
     1261 acaaaaaaga tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct
     1321 atctttactt atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta
     1381 taaaaagaac aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa
     1441 tgatcataat cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg
     1501 cctttgagaa aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa
     1561 ttccgatcgg cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat
     1621 acaatcccat atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac
     1681 caatcaatcc gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata
     1741 attgagcgcc gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca
     1801 catcagaagt atctcttcct tttttccctg cttctttcca taacgcagga acagttccag
     1861 cgatacatcc gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga
     1921 ctaaaaaaga aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca
     1981 aaacattttc tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca
     2041 tgccaaagac agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga
     2101 taaacattcc cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat
     2161 tttccatttt ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc
     2221 atgattgaac atttcctgct attttactta aagacttttt cataggtctc tattattttt
     2281 tcattcattt tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg
     2341 ccattgtaaa gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct
     2401 aaaagtgggc tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg
     2461 ccatgatttt ttacataggc aatataatct ttcccaaaat tatacttgga cagctgtcca
     2521 taaatcgcat ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg
     2581 atcaatactg tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct
     2641 ctgcatgaga tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt
     2701 ctcgtagttt tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc
     2761 cactcgtttt accgaacgat aagttcttcc tcctaaaaac aacaccgcac caataaccgt
     2821 cagaatgatc atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc
     2881 acctagttat tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct
     2941 tccttgattt ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt
     3001 acgaaaatga tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat
     3061 actttacaca gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg
     3121 gagcgaaatt tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa
     3181 tgacggcagc ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt
     3241 gtatctagta gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt
     3301 tcagttaatg gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga
     3361 ttttcttgga tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa
     3421 aaaagttcat cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc
     3481 tgccagagtc atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc
     3541 aagtcttcgc aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga
     3601 aaatgattta aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat
     3661 caagctaaaa cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt
     3721 gaaagaacaa ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct
     3781 tcaatttctt tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata
     3841 ccatacaatg gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca
     3901 ttgtcattat tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag
     3961 gagaaacgtt tgccactata cctgtatgga aaagcggact aatgctattg attggtctta
     4021 tctctgtgtt acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc
     4081 agaatcctcg ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg
     4141 gtttcggcgg gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga
     4201 gcagaaacgt gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta
     4261 tttacagtct gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc
     4321 aatattggat atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta
     4381 ctttcaaaaa gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc
     4441 tagtttcatt gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa
     4501 tggaaatccg gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag
     4561 gaatcgtgtc tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt
     4621 tgtccgctat tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt
     4681 tctactacat cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca
     4741 cttttgatca gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa
     4801 tcgtggttgt cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc
     4861 aagcttttac agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta
     4921 tccaatttcc tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag
     4981 cacgtgtgaa acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg
     5041 ttttattaag tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca
     5101 ttcttgcttc taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta
     5161 cgatcgatgg aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca
     5221 atctgccaga tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg
     5281 aaaagatttg gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat
     5341 ttgttcattt ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg
     5401 ccctgaagat ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag
     5461 ataagcggat gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt
     5521 gcttcaataa taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag
     5581 aagcaatcga agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg
     5641 aaacaagcga agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg
     5701 gggaagaagc ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca
     5761 ctcgtgctgt cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac
     5821 cattttctac tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac
     5881 gaaaggaaaa agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta
     5941 ttgcagttgt caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt
     6001 acacgaacaa taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt
     6061 ctggcagtat ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc
     6121 aatggttcaa tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt
     6181 tacaggaaag gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg
     6241 gattaagaga atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt
     6301 ctagagatag ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag
     6361 ttgagcagac acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa
     6421 aactgcatac cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa
     6481 agttcatgct tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga
     6541 aatcctgttt ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg
     6601 cgtttcttat gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg
     6661 aattgagaat gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt
     6721 cacgttgtca ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac
     6781 atcagttgtg cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc
     6841 accttcttct tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga
     6901 acctggttta gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa
     6961 cacgcctacg ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc
     7021 tgttacaact gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac
     7081 acgaacagca ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa
     7141 gacgtcttcc actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata
     7201 ttcatctact gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa
     7261 tgcttttaat gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct
     7321 caataactcg cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc
     7381 aactttgttc aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg
     7441 ttcacgtgtt tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat
     7501 ttgtgctgcc cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg
     7561 cgcgtagtgg cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg
     7621 ttcacgttct tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt
     7681 tttgcctaat actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc
     7741 aatcgtacca atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta
     7801 caaacctcct aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt
     7861 ctttttttga ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa
     7921 gcttattata cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg
     7981 aaaaaagtac cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc
     8041 ttaactttct tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc
     8101 caacaacttt caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta
     8161 catgaaaaaa ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc
     8221 agcttatcat tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga
     8281 aatgtcagct acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc
     8341 ggcttataat aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc
     8401 attaaagatg aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg
     8461 ggaagaaata aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta
     8521 aatggtctta aaattttatt agataaaaag aggctgggac agaagcgttt aact
//
LOCUS       DACCSK010000104         8466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCSK010000104 DACCSK010000000
VERSION     DACCSK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.106"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(177..1130)
                     /gene="menA"
                     /locus_tag="IVP39_002201"
     CDS             complement(177..1130)
                     /gene="menA"
                     /locus_tag="IVP39_002201"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7587261.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1143..2246)
                     /locus_tag="IVP39_002202"
     CDS             complement(1143..2246)
                     /locus_tag="IVP39_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7587262.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2336..3271)
                     /locus_tag="IVP39_002203"
     CDS             complement(2336..3271)
                     /locus_tag="IVP39_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7587263.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3573..5507
                     /locus_tag="IVP39_002204"
     CDS             3573..5507
                     /locus_tag="IVP39_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7587264.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5964..6375
                     /locus_tag="IVP39_002205"
                     /pseudo
     CDS             5964..6375
                     /locus_tag="IVP39_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
     gene            6389..6955
                     /locus_tag="IVP39_002206"
     CDS             6389..6955
                     /locus_tag="IVP39_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7587265.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6978..7964
                     /locus_tag="IVP39_002207"
     CDS             6978..7964
                     /locus_tag="IVP39_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7587266.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8119..>8466)
                     /locus_tag="IVP39_002208"
     CDS             complement(8119..>8466)
                     /locus_tag="IVP39_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7587267.1"
                     /translation="TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDT
                     RHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIG
                     LRITFMSYKKSKR"
BASE COUNT         2503 a         1636 c         1703 g         2624 t
ORIGIN      
        1 aatttttctt aatactcgaa actaagcgac tttttcacaa cctcctgata aacggtgttc
       61 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatataagga gctattttta
      121 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
      181 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      241 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      301 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      361 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      421 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      481 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      541 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaatc aaattgtcca
      601 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      661 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      721 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      781 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      841 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      901 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      961 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
     1021 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
     1081 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1141 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1201 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1261 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1321 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1381 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1441 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1501 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1561 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1621 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1681 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1741 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1801 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1861 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1921 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1981 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     2041 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     2101 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2161 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2221 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2281 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2341 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2401 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2461 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2521 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2581 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2641 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2701 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2761 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2821 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2881 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2941 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     3001 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     3061 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3121 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3181 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3241 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3301 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3361 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3421 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3481 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3541 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3601 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3661 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3721 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3781 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3841 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3901 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3961 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     4021 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     4081 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4141 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4201 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4261 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4321 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4381 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4441 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4501 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4561 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4621 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4681 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4741 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4801 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4861 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4921 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4981 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     5041 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     5101 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5161 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5221 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5281 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5341 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5401 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5461 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5521 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5581 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5641 tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc ttcttcgaaa
     5701 aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct tcttatttct
     5761 tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc tgctgcttct
     5821 cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct tcgttcttat
     5881 tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat tgggtgagtt
     5941 aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa agtctgctca
     6001 aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta ccagtcatga
     6061 tttttggtct tcagcaaaac accgcaagaa aaagaagcgg tactgcgtgt ggatggcaca
     6121 gaaatcaaaa cattcccttt gatggctgga gaagagagca acacatatac ttacaaagat
     6181 gaacacggag attataactt gatcgagata gatggcgatc gagtacggat aaaagaagca
     6241 gattgcgatg accagatctg cgtacgtcga ggctgggcaa cgaaaaatgg cgaaaccatc
     6301 gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa cagatggagg tgacgaagac
     6361 agtctgattt attagacctt cacacaccat gagtaaatta caaaaaatga tttatatttc
     6421 gctacttgtt gcacaaggag tgatcattgg tttattggaa aatatgatcc cttatccttt
     6481 ttcttttgcg cctggtgcaa aactaggctt agccaatcta atcacgatcg ttgccatttt
     6541 taccatgaaa aaacaagaca gcttcttact actttggcta cgactattcc tgactacttt
     6601 gctaggcgga acgatttcca cattccttta tagtatgagt ggtgctttgc ttagttattt
     6661 tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc agtatcatcg gaattagtgc
     6721 ggccggcggt ttcatgcata atgtcggaca gcttttgatg gcttctttca ttgcacaatc
     6781 atggacagtc atgttgtact tgccagtcct ttcagtttta gggattttat cagggattgc
     6841 aatcgggatc gcagcgaatt atcttttacg acatgtgcag acacttcaac gattccaaaa
     6901 agattatgag cagggaacca aacaaaaatg gagcccaatg tttaccaaaa aataatcgtc
     6961 acacaaggag tttttttatg aagctacatc caatgtggga aaactatcct gctttaagta
     7021 aagaattgac agaaacacta aaacggatgg aagaaactgt ccgcctgaaa aacaaacctg
     7081 tcgaacaagc catcaaagaa acgatccatg ccggcggaaa gctgttgcgt cctgcctatc
     7141 agctattgtt ctcacaattt ggaccagagc aagatcgaga caaagccatc gctttggcag
     7201 cttctatcga aatgctccat acggccacac tgatccatga tgatatcgtt gatgaagcgg
     7261 atctgcgacg tggtcagcct aatatccgta gtcagtttgg taatgtcgtt tctgtctatg
     7321 caggagacta tttatttgtc tgctgtttta aactattgtc tgattactcc acttccttga
     7381 aaagtctcca attgaattct cgcagtatgg aaaaagtatt gaatggcgaa ttagggcaga
     7441 tggatgatcg ctataattat gaactttctg taaaagaata tttggaaaat atttcaggta
     7501 agacagctga actgttccaa ttaagttgtt ctgttggcgc ttttgaaagt ggaacaagcg
     7561 agcgcttcgc aaaaaaagca ggagatatcg gactttctat cgggatggcc tttcagatta
     7621 tcgatgatat tttagattat acaaaagaga gccaggaaat cggcaaacct gtactagaag
     7681 acatgcgtca aggggtctat tctttaccgt tgatctactc tttgcaaaaa aacaaaccaa
     7741 agctactacc ttatttagag aagaaagcgg caatgacaga agaagatgtc gacgccgtac
     7801 gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag gaagttagcg gcctcttaca
     7861 cagaaaaggc tttgaaggaa ataaagaaat tacctgccac ctctcttcga acaaaagaga
     7921 atctcttttc ccttacccaa ctgattttgg atcgcaaaga ctgaataggg aaaggttcga
     7981 tacgtaaata aatttctcag aaggaaatct acgtgttttt tgacaaattg aattgtagtt
     8041 cttgtattta tgataaaaag caaaaataaa gagcaacgtc cgttagcgac gttgctcttt
     8101 tttatcttga atattttttt accttttcga tttcttataa ctcataaaag tgattcttaa
     8161 accgattaca agtaatataa cgcctgcgag tgttatccaa ttattcgatt gacttccagt
     8221 tttaggaagt gctctttttg atttgtttcc attgtcattg cttgttttat tagttaagtt
     8281 aaagttcgtc ctgttatccg aacttgtttt tgagaaggag acacttgcat gacgtgtatc
     8341 gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt gatgaatctg agctatcact
     8401 tgtggtagag gatgtatctg tactgtcact tgaagctgac gtgtccgtgc tatcacttgt
     8461 agtggt
//
LOCUS       DACCSK010000105         8335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCSK010000105 DACCSK010000000
VERSION     DACCSK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.108"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            264..1457
                     /locus_tag="IVP39_002209"
     CDS             264..1457
                     /locus_tag="IVP39_002209"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7587268.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IVP39_002210"
     CDS             1587..3059
                     /locus_tag="IVP39_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7587269.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IVP39_002211"
     CDS             3142..3930
                     /locus_tag="IVP39_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7587270.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IVP39_002212"
     CDS             complement(3997..4665)
                     /locus_tag="IVP39_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7587271.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IVP39_002213"
     CDS             4780..6492
                     /locus_tag="IVP39_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587272.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IVP39_002214"
     CDS             6495..8267
                     /locus_tag="IVP39_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587273.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2551 a         1391 c         1828 g         2565 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggca
//
LOCUS       DACCSK010000106         8297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCSK010000106 DACCSK010000000
VERSION     DACCSK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.109"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..665)
                     /gene="nth"
                     /locus_tag="IVP39_002215"
     CDS             complement(<1..665)
                     /gene="nth"
                     /locus_tag="IVP39_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7587274.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKK"
     gene            complement(672..1370)
                     /locus_tag="IVP39_002216"
     CDS             complement(672..1370)
                     /locus_tag="IVP39_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7587275.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1638..2822
                     /locus_tag="IVP39_002217"
     CDS             1638..2822
                     /locus_tag="IVP39_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7587276.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2828..3271)
                     /locus_tag="IVP39_002218"
     CDS             complement(2828..3271)
                     /locus_tag="IVP39_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7587277.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3497..3784)
                     /locus_tag="IVP39_002219"
     CDS             complement(3497..3784)
                     /locus_tag="IVP39_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587278.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(3955..5307)
                     /locus_tag="IVP39_002220"
     CDS             complement(3955..5307)
                     /locus_tag="IVP39_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7587279.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5580..6026)
                     /locus_tag="IVP39_002221"
     CDS             complement(5580..6026)
                     /locus_tag="IVP39_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7587280.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6262..7188)
                     /gene="cysK"
                     /locus_tag="IVP39_002222"
     CDS             complement(6262..7188)
                     /gene="cysK"
                     /locus_tag="IVP39_002222"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7587281.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7328..8215)
                     /locus_tag="IVP39_002223"
     CDS             complement(7328..8215)
                     /locus_tag="IVP39_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7587282.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2238 a         1713 c         1388 g         2958 t
ORIGIN      
        1 ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat
       61 agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa
      121 atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct
      181 tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc
      241 acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct
      301 gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca
      361 tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac
      421 aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg
      481 ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc
      541 tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca
      601 tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt
      661 aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt
      721 tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct
      781 tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg
      841 taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc
      901 ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc
      961 gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt
     1021 ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg
     1081 taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat
     1141 ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc
     1201 atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt
     1261 agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga
     1321 ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct
     1381 ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat
     1441 ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt
     1501 tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag
     1561 aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt aacattattg
     1621 cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg
     1681 tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa
     1741 cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg
     1801 tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg
     1861 ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg
     1921 gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag
     1981 gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca
     2041 aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag
     2101 ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt
     2161 tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt
     2221 cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga
     2281 tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc
     2341 ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg
     2401 gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc
     2461 gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc
     2521 tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg
     2581 ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat
     2641 ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat
     2701 ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc
     2761 ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat
     2821 agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg
     2881 tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt
     2941 gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga
     3001 tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt
     3061 ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt
     3121 taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg
     3181 caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat
     3241 attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat
     3301 tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa
     3361 accaagaaaa aaaggggttg acaaagcttg tcataacccc tttttctgat taaaagatag
     3421 taaagagaca gtcatcggta attgtgatga ttttttgtca tcacttaagg atgagcggcc
     3481 ttttcaaaaa ctgtctttaa agatcatcat cagtttctag cacatatggc tgaccttcat
     3541 atgtgtactc tgtattcaat gtgccgtcat tttcatctga gtagatgcgt ctactgaaag
     3601 gaaaacgatg agaaacatta tctttggcgt catttgctgg catcgtgtaa tattcctctt
     3661 gcgtttcatt caactgagag agtacttgat ctgccaactc atcaatattg atcgttaatt
     3721 tcttcgaagc acttttataa tcttctaaaa atgcatcttg ttggacaaaa tattctttat
     3781 tcatcttctt tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc ttttatgtaa
     3841 cagctttatc tttgctgctt attgtgacaa aaaattcgaa gaaagacaac taattgtact
     3901 tttcacaaaa aaagacagag ggtcgtgaca aaattgtgtc acaacccttt ttctttattg
     3961 tgcagtttcg atttcgccta gcgctgcttg tgcaagtagg ttcaagtaat tccatgggcg
     4021 gtcaaaatgt ggttggaaga agaaatctga aagtgcgaga tcttctacag tcattttatt
     4081 ttgaacagct aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg acatgaactg
     4141 agcacctacg atacgattcg ttcctttttc atagactagc tccatcatta ctttttcagt
     4201 agttggcatg aactctggac gataattatc ttcaaataca gtcgcttgga cttctagatc
     4261 gttcaaagtt gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc caaataggta
     4321 aagtcctgaa gttccttgag taccacgata cgccatttta tgatctgtca agttatttcc
     4381 cacaagcaaa ccttgacgaa cggcatttgt tgccaaagga atatagtttt gcgtgccact
     4441 tggattgtag ttgactactg cagagtctcc agctgcaaaa atgtctggat tacttgtctg
     4501 catgtattca ttcactttga ttgccccgtt tggcagtgta tctacttttc cttcgatcag
     4561 ttttgtattt ggacggaagc ccacacataa aatgaccata tctgtatcaa atgtatctgt
     4621 tggtgtagct acttgtttca cttttccttc ttcatctgtg ataaattcag taacattttc
     4681 gcctagcgca agagtaacac cgcggtctgt caattctttt tctaagatat ctgtgaatgg
     4741 tttatctaga tatttattca agatgcgatc taatccatca atcaatgtca cttgttttcc
     4801 agattcgaca aaagcttcta ctaattcaat accgatatat ccaccaccaa caattactac
     4861 acgttttgct tctttcgctt gtgcaataat ttcatttgct tgattgtagt ttttgcatag
     4921 gagtacattt ttcgcatcga ttccactaat cggtgggata ataggccatg aaccagttgt
     4981 catcactaat ttatcataag aaaccgtttc tgtttctcct gttttcagat cttttgccgt
     5041 tactgtttta ttttctgtat cgacgtctgt tacatcgtgt tccattttaa ctgttgctcc
     5101 taatgaagat aattcttcag gatttgagta gaacaagcct gctgggtcct ttactactcc
     5161 gccgacataa agtgcgatac cgcaagataa gaaagaaata ttgtcatttc tttcatatac
     5221 tgtcacttct gcttctggat gattttttaa aatacttttt acagctgcag tacctgcatg
     5281 agtacagcca attactacta ctttcattta tggtccctcc gattaacatt ttgtccttcg
     5341 acaaaactac tataacagaa ataaaacgga tacaacagtt taatgcttgt gaattcacaa
     5401 aggtttgttt gtagtttcat tccttttcaa gcataagaac ccaatatcta caagattctt
     5461 attttaataa tattcacttt tatatctaag attgtgaata aaagacaaaa aaataaaaaa
     5521 gctagcgttt tttttacaca gctagctttt tttaggtata acacttgtag aaactgtttt
     5581 tacatttgcg ccttttttgc tgactcttta caatccggac agagtccata caattccaac
     5641 cgatgttcat taatctcaaa atcagtcagt ctgcttgcag ccatttctat atcttcaagc
     5701 cctgggtaat gaaaatcaac aatcttaccg cagttttcac aaattacatg ataatgacgc
     5761 tttgaactga aatcaaatcg gctagaagca tcgccgtact tcatttcttg aacaaatcca
     5821 atcgaagtaa ataagcgaag gttattgtaa acagtcgcaa cactcatatt tggaaaccga
     5881 tgttcaagtg ctttataaat ctcatcagcc gttggatggg aatgatgttc aatcaaatat
     5941 tctaaaaccg cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa cttttcgata
     6001 acctcttcga ctaacttatt ttccatgtaa acaccccacg ttgttattct cgtcttatca
     6061 tactattttt acattttaaa atccacttaa catgaaaaga aaattcaatt ttattgtatt
     6121 ttaatgaaaa aagacgtaaa acagtcgcgt tcactattct acgtctctat tcaaataatt
     6181 tttctcatca taaatttttg tgtgttcttt tcatctttaa aagtttttta cataaaccta
     6241 tctggagaaa aaccaagtgc cttatatctc ttggtaaagg gctgtagata aataacgttc
     6301 accattgtcg ggaaccacag ctaatacctt cttgcctttt cctaattctt ttgcgacttt
     6361 tagcgcggca gcaattgcag cgccagatga gatgcctact aaaatccctt cttttcgccc
     6421 aacttctcta gctgtttcca tcgcttcgtc tccactaata gaaaggactt tatcatacac
     6481 agaagtatcc agtgttttag gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc
     6541 cgcttctttc ccttctaaaa tagcagattc tgctggttct accccgatta attcgatttt
     6601 cggatacaca cgttttaatt ctcggccagc gccagtgatc gttcctcctg taccgatacc
     6661 tgcaacaaac gcgtccaagc catttactcc aaatgcttga tgtatctcag gaccagtcgt
     6721 cttctcatgc acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt
     6781 ttcttttgcc aaacgttctg cttctctgat tgatccagaa ataccatccg caccaggagt
     6841 caaaatcaac tctgcgccgt accctttcat caataaacgt ctttcgatac tcatcgtctc
     6901 aggcatcacg atgattactt tatatccttt tgcaactcct accatcgaca atccgattcc
     6961 tgtatttcca gaagtaggtt caatgatagt atcacctggt ttcagtagac catcgtgttc
     7021 tgctttttct atcatactta aagcaatacg atcttttaca cttcctcctg gattaaaaaa
     7081 ctcaagcttt acaaaaacat ctgctgaatc ttctggtacg attttattta atttgacgat
     7141 tggcgttttg ccaatcaatt cggtgactga gttatagata gctgtcatct tcaattcctc
     7201 cttgtctttg ctcttcttac ttatcataat acaatctgtt catttatggc gagtgattcg
     7261 tctttgacta gcaaaaaaaa gacaaagata taccatatat ggtaatttct ttgccttttt
     7321 atctaaatca aacgtttagt tcttgaccaa ctaataaaga ataactggac atagacatgc
     7381 catttttcgc cattagatca tcaacagaca tgccaaattt ttgtgcaata tcccatagcg
     7441 tatcacctga cacgactgta tatgtctgac cattatttgt atttacatct gttgttgaag
     7501 atgatgacgt tgaagtcgtc gtagtagtcg ttgttgttgt acttgtggtt gttgtagccg
     7561 aacctgttga tggcgtatca aaacgggtta aattataagt attaatcaat gagttcaatt
     7621 tggccccata atttggatcg gtagcataac gacctgtcaa gtacgctgtt gcatcataaa
     7681 aacttgttgt attgcttttc cacacgccag cataatgata atcgcccgtg gaaaaactgg
     7741 ttgaacgaag aacattcgca tggtcttgga aggattccca ataagaagaa tattttcgga
     7801 atggctcatt catcgttacc cattgtccgt ttacatattc tttggttgcc atataaacga
     7861 cctgtccgtt atagcttcct ttcacaccga atagattata atttggcgca ctagctaatc
     7921 cggaagtgcc atatgcactt tccaacaacg cttgagcgat catgactgaa gcatacaagt
     7981 cattacttgc agcaacttct gacgcagacc agccgatttg atcgataaac gcttgaggat
     8041 cagcgactgt ttgagatcct tgttcatctg catgaacatg ttgcggtacc gctgtaacca
     8101 tcatcggccc aactaaaaga ctcgttccaa ttaaaggagc aatcgctttt ttattgatca
     8161 ccatttttct tttaggtcta tggtgccggg cactgcgggt cttcagctct tgcattcaaa
     8221 ttcctcctca aaataaaaaa acttaataaa aagttctctt cttaaaacaa acttaattaa
     8281 ttaaattata cttttaa
//
LOCUS       DACCSK010000107         8290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCSK010000107 DACCSK010000000
VERSION     DACCSK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.110"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IVP39_002224"
     CDS             complement(45..1511)
                     /locus_tag="IVP39_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7587283.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IVP39_002225"
     CDS             1898..2494
                     /locus_tag="IVP39_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7587284.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IVP39_002226"
     CDS             complement(2552..3034)
                     /locus_tag="IVP39_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587285.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IVP39_002227"
     CDS             complement(3140..3337)
                     /locus_tag="IVP39_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587286.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IVP39_002228"
     CDS             3632..4390
                     /locus_tag="IVP39_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7587287.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IVP39_002229"
     CDS             4450..4878
                     /locus_tag="IVP39_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587288.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IVP39_002230"
     tRNA            4924..4996
                     /locus_tag="IVP39_002230"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IVP39_002231"
     CDS             complement(5146..5517)
                     /locus_tag="IVP39_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7587289.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IVP39_002232"
     CDS             5732..5917
                     /locus_tag="IVP39_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587290.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IVP39_002233"
     CDS             complement(6000..6188)
                     /locus_tag="IVP39_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587291.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IVP39_002234"
     CDS             complement(6212..6475)
                     /locus_tag="IVP39_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587292.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IVP39_002235"
     CDS             6633..6890
                     /locus_tag="IVP39_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7587293.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IVP39_002236"
     CDS             complement(6961..7152)
                     /locus_tag="IVP39_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587294.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IVP39_002237"
     CDS             complement(7293..7613)
                     /locus_tag="IVP39_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587295.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IVP39_002238"
     CDS             complement(7636..8070)
                     /locus_tag="IVP39_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7587296.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2628 a         1635 c         1314 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DACCSK010000108         8253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCSK010000108 DACCSK010000000
VERSION     DACCSK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.111"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            1..357
                     /locus_tag="IVP39_002239"
     CDS             1..357
                     /locus_tag="IVP39_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587297.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            373..723
                     /locus_tag="IVP39_002240"
     CDS             373..723
                     /locus_tag="IVP39_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP7587298.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            752..1531
                     /locus_tag="IVP39_002241"
     CDS             752..1531
                     /locus_tag="IVP39_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP7587299.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1542..2219
                     /locus_tag="IVP39_002242"
     CDS             1542..2219
                     /locus_tag="IVP39_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP7587300.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2238..3344
                     /locus_tag="IVP39_002243"
     CDS             2238..3344
                     /locus_tag="IVP39_002243"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP7587301.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3334..4425
                     /locus_tag="IVP39_002244"
     CDS             3334..4425
                     /locus_tag="IVP39_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP7587302.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4438..5178
                     /locus_tag="IVP39_002245"
     CDS             4438..5178
                     /locus_tag="IVP39_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP7587303.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5466..7406
                     /locus_tag="IVP39_002246"
     CDS             5466..7406
                     /locus_tag="IVP39_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7587304.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7493..>8253
                     /locus_tag="IVP39_002247"
     CDS             7493..>8253
                     /locus_tag="IVP39_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7587305.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILA"
BASE COUNT         2697 a         1343 c         1919 g         2294 t
ORIGIN      
        1 atgattaaag tagttattgc agaccgtatg ggtaaaggac aaaatgtggc caaaggggta
       61 gaagcagctg gtggaaaagc agttgtcgta ccaggaatgg gggcagatat gcgtttgggc
      121 gatgtgatgc aacaagagca tgcagatatg ggtatctctt tttgtggtag tggtggtgca
      181 ggtgcattaa cagcagcaac aaaatatggc tatccagaac gacatggtat gcgttcgatt
      241 gaagaaggga tcacagctat caaggatggc aaaactgttc taggtttcgg ctttatggat
      301 caagaggaat tagggaaacg tctgacagaa gaatttatca aaaagcatgg aaaatagaaa
      361 gagggacatg aaatgagcgt tcaaaagaca attcagcaaa aagttatcgt ctccggtaaa
      421 ggggaaacaa agcaacatgc ttttgcatca gcgttagcag aaattcaaaa caaaataatg
      481 gaacaatcaa acgtattatt gcgaatcgaa ccagtcaatg tggatatttt aaaggcggtc
      541 gaaaacaaat acacagaacg ctttctattt ttcttcttcc cacggcaacg tattagctat
      601 gaagtcgaac tctctgttac tgtggctgta acagaaataa aattggcaga catatcgttc
      661 agccagcaaa tgactgaaga tccaaatggg attgccattc catttgtaac taaaaaaatc
      721 taattttgaa tcataagggg gaaagaataa cgtggatttc gtaatcattc tagtcaaatc
      781 ggtcattatc gggggattac ttggttttgc agcatctgtc ggggcagctc gaatgtttca
      841 tgcacctaat acacaaggtt taggagcttt tagaacatta ggcgaaatga atgcctgcat
      901 gggtgatcca gcttctcatt tttcatttgg tttaggcttt ttctttaatg catgggcttc
      961 ttcagttggt gctggtgcat tcacacaaga tgtgacgcat cgaatcattc ccaattgggc
     1021 agcggcagca cttctagcaa aaaacaaaga tgtcacccag accatgcatg atccgaaaaa
     1081 aatggggatc attggagcgg ttatcggggc agtggtggta gccttcttaa acacaacagc
     1141 tgcagcaatt ccagaggcgt tacaagtgac ggcggtttcc gttttggtac cagcagcgac
     1201 gattctgatc aatacagtaa tgccagttat tttttggttg gcagctttag acgcagggaa
     1261 acgaactggt ttttggggca ctctttttgg tggtatcgcg caattgatta tgggaaatgc
     1321 ggtacctggt gttgttctag gaatcttgat tggtaaaggc gtcgatgaga gcggttggaa
     1381 tcgcgtcacg aagagcatga tgatcgctat tgttttatta tttattttca gtggattttt
     1441 ccgtggattt gatatggaaa tgatcaagag cttccagctt caagtgccta attggcttga
     1501 taacttgcac aacatcttta gtgtgaaata ggaggagaat catggagaca acagaagaac
     1561 aagcaaaagt attagaagca aagcggcaaa agagcttttg gtttgcagac tggtcttttc
     1621 cgctaatcgt aggaattatg tccgctgctg tatttgcagg aacacacatg tatgtgatgt
     1681 atggtgtggg tgcattcaat gaagtttcga tcgtggcgat gttaaaagcc gggctggatg
     1741 gcggatccta tggcgcagcg gcagcgtttg gtgctagttt cttatttgcc agaattttag
     1801 aaggctcttt agttgggatc ttggacttag gcggttcgat cctaactggg atcggaattg
     1861 gggtaccagc aattctctta agtatgaaaa tagcggcacc aatcgataat tttgcactct
     1921 ctttattgac tggtttggtt ctaggtttga ttgttggtgg catcattgtc ttgattcgta
     1981 aatttacgat caatcaatca aattccactt ttggtgctga cgtcatgatg ggggcaggaa
     2041 attcatctgg acgttttttg ggtccattaa ttatattaag cgcgtgtgca gcatctattc
     2101 caatcggcat tggcgctaca ttaggagcac taggattcta tgcttggaaa aaaccaattg
     2161 ctggaggtgc tattttagga gcaatgattt taggagcaat cttcccggca gtaaattaat
     2221 agaaagaggg tcattaaatg tttgatactt tgattaaaaa tggaaggtta gttgatggat
     2281 ctaaaataga ggtcgctata gaaaaaggca tgattaaagc agttgcagca gaaataaatc
     2341 agccggcgca agaagtagtt gacttggagg gaaagtatta tttatccgct gggtggatcg
     2401 atgatcatgt ccactgctat gaaaagatga atctttatta tgactatcct gatcagatag
     2461 gggtcgaaaa aggtgtgacg acggtcattg atgctggaac tacgggagca gaaaacatcc
     2521 gtgaatttta tcaattgacg aaatcggtca agacaaatgt gtatgcgcta atgaatatct
     2581 ctaaatgggg cattgtcaaa caagatgaac ttgctgatct aacaaagatt caagaagatc
     2641 tagtaagtac tgcgttagca gaactgccgg aatttattgt cgggattaaa gcgagaatga
     2701 gtaagacagt tgttggtgaa aatggcatta agccattaga attagctaaa aaaatccaga
     2761 cagaaaacaa tgatctgcca ttgatggttc atatcggttc tgcacctcct gagttagaag
     2821 aagtattgtc aagaatggaa agaggcgatg tattgaccca ttgttttaat ggaaaaccaa
     2881 acggtatttt agatccaaag acagatcaaa ttaaaaactt tgtctgggat gcttacaata
     2941 atggtgtcgt ctttgatatt ggacatggga cggatagctt taatttccat gtggcagaaa
     3001 ccgcactaaa agcaggaatg aaagctacct ctattagtac ggatatctac atccgcaatc
     3061 gtacaaatgg cccagttttt gatctagcaa ctactttaga aaaattgagg gtagtcggct
     3121 attcttggga agaaatcatt gaaaaagtta cagcagttcc tgcacggaat tttcatttga
     3181 agaataaggg ccagttagct gtcaattatg atgcagattt gacgatattt gagattgttg
     3241 aaaatgaaaa ggaattgaaa gattccaacg gatttacacg tatagctaaa gaacaaattt
     3301 taccgattaa aacaattatt ggaggcgtca tatatgacaa ttagctatgg aaagtttgat
     3361 ttaaaagaag tcattaatgc atcgggaaaa atgacgatcc taggggtttc taaggtatct
     3421 gaaagtgtcc tagcagcaca acgatttgga ggcgagcatt tttttgagat gagtgatctt
     3481 gccatcaaaa ccggcgctta tctttctcaa ttactgaaag tggaagatgc gcaaatcgtt
     3541 tcttctgctt cagcagggat tgctcaatct gttgctgctt taatcggaca gggcgacatg
     3601 taccatgtct atcatcccta tacagaaaaa atcgctagac gggaaatcat catacctaag
     3661 ggacataatg tagattatgg gacaccggtt gaagtaatgg ttgctcaagg tggaggaaaa
     3721 gtcatagaag ctggctatgc aaatatgtgt acaccggaac atgtttccat gatgattacg
     3781 gaaaaaactg ctgcgcttct atatattaaa agtcatcatg cagttcagaa aagtatgctg
     3841 acggtggcag aaatggtaga agttgccaaa gctcatcgtt tgccattgat cgttgatgca
     3901 gctgcggaag aagatttatt caaatatagc gaaatgggcg ttgacttagt gatttactct
     3961 ggggcaaaag caatcgaagg tccgagtgcc ggactggtca taggaaaaaa gaagtatatt
     4021 caatggattc ggctacagtc taaaggtatt ggccgagcaa tgaaaatcgg taaagataat
     4081 attttaggct ttacccaggc actagaagat tatttaaaaa atgggagcga atccggtgac
     4141 tccatgaagc aacgattagc gccttttgtt aacgcaatca atcaacttcc tgacctttca
     4201 gcgaaaatag ttcaagatgg tgccggcaga gatatttatc gagcaagtgt aacgattaat
     4261 ggcgataaaa gtgcgaaaga agtcattcag gaattaaaag cacgaaatcc agcagtctac
     4321 acccgagaat atcaagcaaa taacggaatc attgagttcg acattcgttc cgtcagccaa
     4381 aacgaaatgg aaaaaattat aaaaagattg agagaaatca tgtaggaggc ttttaaattg
     4441 tcagttatcc caaattatta taaaaatcgt gtatgtttaa atgtattagc tcattcagtt
     4501 gaaaatgcaa aagcatgcta tgaagcagcg gaagggcatg tcgtactagg tgtgctatcg
     4561 aaaaattatg ctagcgatga agcagcaatc gaagatatga aaaggtacca agacgcaacc
     4621 aataatgcgt tgtcagttgg attaggcgct ggagatccaa accaaagtca gatggtttcg
     4681 cgtttatctg gggttttaca accacaacat gtcaatcaag tatttactgg cgtggggact
     4741 tctcgtgctt tattgggcca aaatgataca gtcatcaatg gattggtttc accaacaggt
     4801 aagatcggct atgtcaatat tgctacaggt ccgttaagtt cacaagcgcc agcagctgaa
     4861 gtgcctattg aaactgccat tcaaatgctg caagacatgg gaggtacttc aatcaagtat
     4921 ttcccgatga aaggcttagc acatagggaa gaatttaaag cagttgcgga agcttgcgca
     4981 aaatatgaat tctatttaga gccaacagga ggaattgatc tttcaaactt tgaagaaatt
     5041 ctgaaaattg caatcgatgc aggtgtaaaa aaagtgattc ctcatgttta tagttcaatt
     5101 atagataaag aaacaggaga tacgcgtata ggagacgtaa agatactatt aagcatggta
     5161 aaaaaaatta ttcactaact cattagaaaa taagaagctt attccagaat gaaattaaat
     5221 ggaataagct ttttataatt aattacggaa attgttaatg tagataataa tttagatgta
     5281 cgtaagcaga agtacagaaa tgtaagattt tccaatatta ttttattcaa cataataaat
     5341 cgtatatcaa tttttttagc gaaatttcta tttattccaa gaatttcagt tattacggaa
     5401 tcattttgtg cttaataaaa agaaaccgtt tttatatact ataaatgtag aaaggcggta
     5461 ggaaaatgaa aaaaattatt gcaatcacat cctgtccagt tggtattgcc catacataca
     5521 tggcagcaga aaacttagag aaagcgggga aagcagttgg cgccgaggta aaagtcgaaa
     5581 cacatggttc catcggtatt gaaaatgaat tgactgcaag agatatcgaa gaagcagctg
     5641 gtgtaatcat cgcagcagat actaaaatag ataaatcacg attcggcggt aaacctttga
     5701 taaccgtagg tgtgcaagaa ggaatccacc atgcagatga gttggtgaga gatattttag
     5761 caggaaaagc gccagtctat aaaagtacgg aggcaattat ttcttccgaa aataaaagtg
     5821 aatcagaaaa tttaggtaaa aaaatctata agagtttgat gaatggcgtt tcgtacatgg
     5881 tacctttcgt agttacagga gggttattga tcgcgatttc tttaacatta ggaggaacag
     5941 ctacacctga agggatcaag ataccagaag gaacgatttg ggcaacgatg aatagtatcg
     6001 gaagtatcgc tatggggttg atggttccaa ttctatctgc atttattgcg caaagtattg
     6061 cagatcgtcc aggacttgtt cctggatttg ttggtgggat gttagcggca aacggagcat
     6121 tatatggtag tgatgcgaat gcgggattct taggcgggat catcactgga tttttagcag
     6181 gattcattgc tttaggaatc aagaaagtgc gagtaccgaa agctttacaa tcgatcatgc
     6241 caattattgt tatcccaatt ttaggatcat tggcagttgg ttttgtgttt atctatgtta
     6301 ttggagaacc agtagctctt ctatttagtt cattgactca ctttttagca gggatgcaag
     6361 gaggaagtga aattgttcta gctatgatct taggagcgat gattgctttt gatatggggg
     6421 ggccgttcaa taaagtagcc tttttgttcg gatctggctt gattgcagaa ggcaattatt
     6481 ccattatggg gccaattgct gtagctatct gtatccctcc attagcattg gggctggcat
     6541 cattgatttt aaaaaccaaa tttacgaaaa cagaacgaga agctggtaag gcgtccttag
     6601 caatgggact gtttgggatt acagaaggcg ctattccgtt tgcttcaaca gatccactac
     6661 gggtgattcc aagcattgta atcggggcaa tgacaggaag tgtgatcgcg atgctttcag
     6721 gagtgacaga tcatgtagct catggcggtc cgatcgtagc ggttttagga gcagtggatc
     6781 acgtagccat gttttttgta gcagtgattg ctggtattgc tgtaaccgtg ctaatgttaa
     6841 gaatccttaa accgacatta caacaaactg cggttgctgc agcaaatggt gtgacagtag
     6901 cagatctatc agagactgaa acaagtgatg tttatacgaa aactgaaact tttgattcta
     6961 tcacggatat tatgtcagat gaagcaatcg ttttaggtga aacagcaaca acaaaagacg
     7021 gagtaatcga agatttagtc caacgtttag aaaaaacagc ggcgatcaca gataaaacca
     7081 gatttaaaga agtcatctat gatagagaac atgaaagtac aacgggaatc ggtatgggga
     7141 ttgctattcc acatggaaaa tcagcttatg ttcttaaacc aacagtggca tttgccagaa
     7201 gtcaatctgg ggtagattgg cattctttag atggaaaacc tgcacatatg atctttatga
     7261 ttgctgtacc tgaaaacagc caaggtgata tgcacttgaa aatactgcaa cgtctatcac
     7321 gtaaattgat ggatgatgac ttccgtcaag cactgatgga agcgccagat aaaacaacag
     7381 ttcaccaatt attaagcgaa atgtaacata agatgaaaaa tcgaccgtga tgagacataa
     7441 tagatgtgtt atcatgatcg attttttatt tagaagggag tttctgcaca ttatgaatca
     7501 gcgtcagcaa tctttgatct atcaattaag tgagaacaat aattatataa caacaaaaaa
     7561 gctagcagag catttccatg tgtcagaaag aacgattcat agcgatttga ataccattga
     7621 aacttggttg agagacaaac atctgccttt aatgtttgaa agaaaaaaag gtacaggtat
     7681 cttgttgaat ggtctgcctc acgaaaggga acaactgaaa cgagcgctta atgaagagac
     7741 tcaaacagaa atggataaaa aacagcttcg tcagttattg attttccatc tgctagtagc
     7801 aaaagacgga tttagtcttg aagaactttc agaaaaacta tatgttggaa aacgtacgat
     7861 tcgtaaagaa ttgacagagt tgcaaagctt ttttgagtat caccatttac agctagtatc
     7921 caaaacaaaa ctaggaactt ttcttaaagg cgatgaacaa gaaaaaagac agttgcttgt
     7981 taagacatta cgaaatatgc agaaagaaga tccacaaagt ccgtcgttga aagaattctt
     8041 cgctaaagat acattaaaag ttatccaaca tacattacaa gatgtttttt atgaaaacca
     8101 tttagaacag ccgaatggac tggctagtgt ggagattcac atctacttta tgctcgagcg
     8161 aatgaaacag taccagaaag tgaaattaag taaagacgaa aacgaagtcg tggaacacac
     8221 ccaagcacaa aagttaagta gtcaaatatt agc
//
LOCUS       DACCSK010000109         7992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCSK010000109 DACCSK010000000
VERSION     DACCSK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.112"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            432..1805
                     /gene="glmU"
                     /locus_tag="IVP39_002248"
     CDS             432..1805
                     /gene="glmU"
                     /locus_tag="IVP39_002248"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7587306.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1899..2870
                     /locus_tag="IVP39_002249"
     CDS             1899..2870
                     /locus_tag="IVP39_002249"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7587307.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2876..2978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3044..3613
                     /gene="thiT"
                     /locus_tag="IVP39_002250"
     CDS             3044..3613
                     /gene="thiT"
                     /locus_tag="IVP39_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7587308.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3694..4467)
                     /locus_tag="IVP39_002251"
     CDS             complement(3694..4467)
                     /locus_tag="IVP39_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587309.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            4734..6434
                     /locus_tag="IVP39_002252"
     CDS             4734..6434
                     /locus_tag="IVP39_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7587310.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            6404..6622
                     /locus_tag="IVP39_002253"
     CDS             6404..6622
                     /locus_tag="IVP39_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587311.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            6642..7532
                     /locus_tag="IVP39_002254"
     CDS             6642..7532
                     /locus_tag="IVP39_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7587312.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            7550..7834
                     /locus_tag="IVP39_002255"
     CDS             7550..7834
                     /locus_tag="IVP39_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587313.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2707 a         1374 c         1677 g         2234 t
ORIGIN      
        1 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatct agaggttgtg
       61 aaatcagcgt cctgtcctga gtattagctc aataaacgga aaaattgctc ctcatatttt
      121 gccgtttatt gagctaatac cgaaggtcag ctgattgaaa aacgtttaat gaagaggttg
      181 taagcctacc gtttaactct aagtcacaag aaacgatttc aaaaaacagc ttttcatgtt
      241 ttatgagtag tgtgacttgt gaccgaggag ttggcaggag agcaccggtt accaaataga
      301 taggcccgtt atggtctatc tattttttta tccagaaaac gctgggataa aagaaaatca
      361 cttgttttgt tgaaaaaatg ggtatactgg gataaactga aatcagcaaa ctatatttag
      421 gagtgacgca gttggacgca cgttatgcaa ttattttggc ggcagggaaa ggtacccgca
      481 tgaagtcaaa attatataaa gttttacatc ccgtttctgg taaacctatg gtagaacata
      541 tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt ggacacggag
      601 cagaacaagt taaagcacaa ctaggagaac gcagtaaata tgcgttacaa gcagaacaat
      661 tgggaacagg acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa aaaggaacaa
      721 cgttagttat cagtggcgat acaccattat tgactactga aacgctgaac aatctttttg
      781 aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca gaaaacccaa
      841 caggatatgg acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc gtggaacaaa
      901 aagatgcaac accagaagaa gctcttgtac aagaaatcaa tacaggaact tactgttttg
      961 acaacgaagc tttgttcgat gcgttaagca aagttggaac aaacaatgct caaggtgaat
     1021 actatctaac agatatcatc gaaatcttaa aagaagaagg ccatacagtt gctgcttatc
     1081 aaacggatga ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta gctaaagcaa
     1141 atgagatcat gagaaagcga attaaccaga tgcatatggt caatggcgta agctttgtcg
     1201 attcagcaac gacatacatc gatgcaggcg tagaaatcgg tccagacaca ttgatcgaag
     1261 caggtgtaca aatccaagga aatacagtca ttggttctga ttgtgtcatc gggtcacatt
     1321 caaaaatcgt agacagtagg attgaggatc acgtcgtcat tgaaaactca gtcattgaaa
     1381 gcagccatgt caagaaacat gcagatgtgg gaccttatgc acatttacga ccaaaagctg
     1441 aaattggcga aaacgtccat atcggaaact ttgtggaagt gaaaaatgct caaatcggca
     1501 aaggaactaa agtcggtcac ttgacttatg ttggtgatgc tacgctggga gaagaaatca
     1561 atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat catacgactg
     1621 tcggcgacca cagctttatt ggttctagca cgaatatcat cggacctgtc gaggtagcta
     1681 aaaacagttc gattgctgcc gggtcaacaa ttacagacaa tattccagaa tatgcgctgg
     1741 caattgcgag agcaagacaa gtcaataaag aaggatatgc aaaaaaactc ccttatctaa
     1801 actaagactt ttgcaaaatc acttgattta tttaaaaaat atgactacta tttattaggt
     1861 agaattctag agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat tactttgacc
     1921 caagattaaa aatttttgcg ttaaattcca atcgcccatt agcagagaaa attgcagcag
     1981 cagtcggcgt cgagttagga aaatcatctg ttactcaatt tagtgatgga gaaattcaag
     2041 taaacattga agaaagtatt cgtggatcgc atgtctatgt tattcaatca acgagcagcc
     2101 cagtcaatga taacttgatg gagttgctga tcatgattga cgcactgaaa cgagcaagtg
     2161 caaaaacaat caacgtagtc atgccttatt atggctatgc gcgtcaagac cgtaaagcac
     2221 gtgcacggga accaatcaca gcgaaattag tagcaaacat gattcaaaaa gctggtgcaa
     2281 caagaatgct gactttggac cttcatgcag ttcaaatcca aggtttcttt gatattccag
     2341 tcgatcattt gatgggtgcg ccacttattg ccaattattt cttagaaaaa ggaatcaaag
     2401 gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt cacacgggca cgtaaattag
     2461 cggaattttt gaaatctccg attgcgatca tagacaaacg ccgtccaaaa gcaaatgttg
     2521 cagaagtaat gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg atcgatgata
     2581 tgatcgatac agcaggtacg atcacattgg ctgccaatgc tttgaaagaa gctggtgcaa
     2641 ctagtgtgta tgcttcatgt acacatccag tattatccgg ccctgcttta caaagaatag
     2701 aagattcagc tatcgagcgt ctagttgtta cagactcgat ctatcttcca gaagatcgta
     2761 aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat gggagatgca atcaaacgta
     2821 tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa aaataaataa taaataaact
     2881 cacaaagggg agtccaattg ggctgagatt gaatctattt ctaaaccctt cgtacctgta
     2941 tcggttatgc gagcgtagga attgtgaata aacagctgtt tgactatttt cgaaagctgc
     3001 ttcctccttt gtgacttttt tattcaaagg aggaagtttt tttatgggaa aaaatagagt
     3061 ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga ttccgatcca
     3121 gatcggcagc agtttttcta tttcattagg gcagattcca ttgactcttt ttgcgttaag
     3181 aagagggaca aaggcaggga tgctggcagg tttcatttgg ggcatcctac attttccgtt
     3241 gggacaagtt tattatttaa gtgtcgttca agtgttgatt gagtacccga ttgcctatac
     3301 ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa aaaggctatg tagacgataa
     3361 tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg attggcgcac tttccagata
     3421 tttttggcat tttattgcag gagtggtttt ctggggagcg tatgcactat gggggatgaa
     3481 tccttggctc ttttctttcg taatgaatgg tgccagcggg ttgagtacag ctattgtaac
     3541 gatcatcgtt ttattaggga tccaacaaac gactccttcg atttttgtgc cagataaacg
     3601 acaggagctg taaataaaaa agaagcaaga aacttgagac agaatcatgt cctagtttct
     3661 tgtttccttt ttattttttg ataagaaata gatctacttc aatggcagat aagaaagatc
     3721 ggcatcttct accaattgcc agtgtcgatc ggtgacagag tagattaaag tagcgatgat
     3781 ttcctcttct cgataccggg tgccttttaa tttccaaata taatcttcaa tcacatagat
     3841 tgggatacga tatttaccat cgatcaaacc aacttgatca aaagaaaaaa tgatcccttc
     3901 ctgcatcaaa ttacgcattt ctttcaataa ttccacactg attttttcaa caggtgtttc
     3961 ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac ttaatttact
     4021 agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca gctgatcatt
     4081 aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat gaagatgtgt
     4141 tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt ctttcagcat
     4201 cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt tacgagaaga
     4261 ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg tatgggattc
     4321 atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa aatgcacgat
     4381 actgtttgca aagtcagtag cagtcagccc ttttgttaaa acagcattac ttgtggtatc
     4441 aatatgtaca taaactgact cagtcatcat gttcctcctc ttatttcctc ttaatctctt
     4501 ttattttaca acatttgcat taaaaaagct tgtttctttt attttaaaat tccattacaa
     4561 aatcacgttt tcattgaaaa cactctcatt ttcaattaat ttttgtgatt tttgtatttt
     4621 tttatttaaa ggaggtttta tgaattgttt tcttatggaa tctacaaagt cgaatacgag
     4681 actcatgacc tttttatgac gtactggtat acttgaagaa aaaggagagc gcgatggaga
     4741 taaaaaaagt ttacttttca cagaaaatgt cgctgtattc gcctatccag caagcaaaac
     4801 aaccccttcc attttttcaa agtcatcaag aaagtagaga cgctttttgg cgttctgcaa
     4861 aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac cgtctatttt actattgcta
     4921 aaagtagagg agatgttatt tttaataatc tgttgattga aggtgaaact tttgaatgga
     4981 aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt tattaaagaa aactgttgtt
     5041 ttttatttca atctcctcta tcagaagaat ggttgcgtat ttttcataaa aacggctatc
     5101 aaggaaccac acaattaaat aaaaagctgg tctatcatac agcacttgtt ttaggtggag
     5161 gaggagcgca cggtgcttat cagattggtg tgtggcaggc gctgaaggaa catgatatca
     5221 actttgagat tatcacaggt acctcggtag gtgcgttgaa cggcgcattg attttacaag
     5281 gagatatgaa aaaagctgtg aatttatgga aaaagctgtc gacaagacaa gtgcttgcgt
     5341 taccagaaat ggctgcagtt gaagaccttc gtgaacgctt ctatcgagaa agacgccaaa
     5401 tgacgaaaac agcactaatt gaaggcgggg tttcttctgc tccattggaa aagatggtca
     5461 aagacaattt ggatttgtcc ttactcaaac ataatcccaa aatacgttta tatacggtgt
     5521 ctactaaact gccagaacta gcggaagtag tgactgatat ccaacagaca aaaacagaag
     5581 aaataccaga ttggatactc gcctctgctt cattttatcc cgctatggct tatcgaaaga
     5641 tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa aatacctatt gatattgcga
     5701 ttaacaaagg agcaacagag gcatttgtcg tagatgtgca agggccaggc ccagcgaaaa
     5761 aaattcgtat gccagacatt tttatccatt ggaaatgtca aacattgtgg acactaggca
     5821 gctttctgct ttttgatagc caaaggaacc aattaaattt acaattaggc tatctggaaa
     5881 tgaaaaaacg attaggctat tactatggga actggtatac atttcattct gttaaacctg
     5941 caagaacata ctggcgagga tttttgtctt acttaacaaa agaaattcag ttagaattat
     6001 cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa tttatataag aacagggtcg
     6061 taccagagac atgcggattg gcgatgctag aacttttagc aaaaaaacat tttttactgc
     6121 ccaatgaaat ctatcaagta gacatgatga ttcaaatgat ctgccaacaa gatataaaaa
     6181 gtatgccgga tgatttattg gcacaaattg gtcaattaag tactgaagaa tggcgaaggt
     6241 atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa aactgtttat tttgattatc
     6301 tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt gcttaagcaa ccagttgaca
     6361 cattactgat cctatattta tattatctca aggaggaaca accatggcac aagaattttc
     6421 atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac aacaaggggt ggcgtaaaga
     6481 actgaaccta gtcagctgga atggtcgccc gccaaaattc gatcttcgag attgggcgcc
     6541 agaccatgaa aaaatgggga aaggcctgac attgacgaac gaagagtttg aacaactgca
     6601 aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg aatgaaaagt atgactggtt
     6661 ttggcaaagc catccgcgaa acaaaagcgt atcagttaga tattgaaata aaaagtgtca
     6721 accagcgttt tctagatgtt caaatacgaa gtcctaaaat cctgaacttt gttgaaaacg
     6781 attttcgtca gcttattaaa cagcagttaa gccgcggtcg aatcgaagta tttgtgaatc
     6841 tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa ctgggaattg attgattcac
     6901 ttatcactca attgacagac ggaattcaac aacgatacgg tagtcaaaat caagtgtcaa
     6961 ttagtcggat ggcggaacgc ttcgcgctgg acgaaaactt cgtaacggta gaagaaaaac
     7021 cggaagagag catggaagaa ttggcacagt tagctctaga tacattgaaa agcgcacttg
     7081 attctattga ggcgagtcga gaaaaagaag gtcaagcttt agctttagtg ttgaaaaaga
     7141 atactgaaga attcaaagaa gtactaaaaa aactcgatag ttttgtagaa atctatgaaa
     7201 aggaatttca aataaggtat caaaaaaagc tggaggaatt tcttggagcg aaagtcgatg
     7261 agcaacggtt gttgacggag ctagccatct tgttggaacg cggagatatt cacgaggaac
     7321 tggatcgtct ggcgatccat gtacaaaaac tagatgaact tatcgaagca caatgtcctg
     7381 taggtcggga attagatttc ctgatccaag aaatgaatcg ggaaatcaac acgatcggtt
     7441 ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca gtctaagacg atcttagaaa
     7501 aaatcagaga acaagtacaa aatattgaat gacgaaagag gtagagataa tgaaatatta
     7561 tgacgtaacg ttccacgaat taagtggaaa aactgtggtt aaacgggata tcccttctga
     7621 aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac tataatgaga acgaactgtt
     7681 tattttgatc aacgacggtg cttacgtgac gatgaatcgg aaatttattg ttcgaattga
     7741 cacagaagag gtagaagatc cgacagaaaa agcacgttct cgaaaagatg agatcatggg
     7801 tgtagtgaat actttgtcca atatgggctt ttagcaagga tgctgaacaa agaaatacaa
     7861 taaataatca gaattggatt tcttcgctta gcttctttgc tctaggaata catttaaagg
     7921 ttgtgacaaa attcgtgtca caatcttttt ttattttgaa taagtgatgt tcaaaaacca
     7981 ttttctcggt at
//
LOCUS       DACCSK010000110         7979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCSK010000110 DACCSK010000000
VERSION     DACCSK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.113"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IVP39_002256"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IVP39_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7587314.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IVP39_002257"
     CDS             1228..2331
                     /locus_tag="IVP39_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7587315.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IVP39_002258"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IVP39_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7587316.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="IVP39_002259"
     CDS             complement(3821..6268)
                     /locus_tag="IVP39_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7587317.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="IVP39_002260"
     CDS             complement(6457..7893)
                     /locus_tag="IVP39_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7587318.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2214 a         1736 c         1358 g         2671 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa ttatgtaaa
//
LOCUS       DACCSK010000111         7922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCSK010000111 DACCSK010000000
VERSION     DACCSK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.114"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(61..936)
                     /locus_tag="IVP39_002261"
     CDS             complement(61..936)
                     /locus_tag="IVP39_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7587319.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1084..1416
                     /locus_tag="IVP39_002262"
     CDS             1084..1416
                     /locus_tag="IVP39_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7587320.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1528..2736
                     /locus_tag="IVP39_002263"
     CDS             1528..2736
                     /locus_tag="IVP39_002263"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7587321.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2890..3345
                     /locus_tag="IVP39_002264"
     CDS             2890..3345
                     /locus_tag="IVP39_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587322.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3707..3982
                     /locus_tag="IVP39_002265"
     CDS             3707..3982
                     /locus_tag="IVP39_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587323.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3995..6073
                     /locus_tag="IVP39_002266"
     CDS             3995..6073
                     /locus_tag="IVP39_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7587324.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6236..>7922
                     /locus_tag="IVP39_002267"
     CDS             6236..>7922
                     /locus_tag="IVP39_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7587325.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYME"
BASE COUNT         2642 a         1336 c         1677 g         2267 t
ORIGIN      
        1 aaaagacaaa ggtcagctgg atgtggaatc tttcctcata gcctatcttt gtcttttttc
       61 ttatagtttt ttcagtaatc ttgtctgtgg cttcaaataa gtaaagcctt gcccttccac
      121 ttcatgcaca ttgatgattg aaaggaaagc tttttcgtca atctcgtgga cgatcctctt
      181 agcttcagtt aattcactag agctaacgac catatagatg attttttttg aattaccaga
      241 gaatgcgcct tctccagaaa ggaaagtcac acctctttgc agaccggtca tcagtagtgg
      301 tgcaatctct tcgcttttgt tggagacgac aagtagacct tttgcagaat atccgccatc
      361 taagatcgaa tcaatcacac gactgaaaac atacgaaaca atcaacgtat acatcatacg
      421 tttcaagtcg atataggtta atgaagcaag taagacgatg atatcaaatg ccaataggga
      481 acgccccata gtgatgccgt aattttgttc taaaatccga gcgataatat cactgccgcc
      541 tgttgttcca cccatgcggt agacgatccc gcttcctaca cctgcaacta aacccgcaag
      601 tagagaaacg atcagtagat catgctgtaa attgatttct agtggaaccc tttgccagat
      661 ccataaaaag aatgacaaag aaagtgtacc tagaacagta taggccaagg aacgtttacc
      721 gaggatcttt cctcctagta aaatcaatgg gatgttgatt aataaagtcg tataagctgg
      781 atcaatacca aataaagctc ttaagatcaa ggtgatccct gagactcccc cttctgctaa
      841 atgattagcg atgttcaagt aaacaagacc aaatgcataa atacttgttc ctataatgat
      901 aaaaagaact tcttttactg tttttcgttt gaccatatga attccagctt tcaattatgt
      961 attttcacag aatttccatt tagaaattcc ttcctaaatg tagcatggaa attcgatttt
     1021 ctcaattgct cttatctagg atttgcgtta aagttttggt aagatagttt tggagggaaa
     1081 aagatggata aaaagcgttc tttatacagt atgcagcagg aagtggatga atatatccag
     1141 caattcaagg tggggtattt ctctccatta gctcagatgg ctcgattaac tgaagaagta
     1201 ggtgaattgg caagagaagt caaccacact tatggagaaa aaagcaaaaa ggcttctgaa
     1261 ccagttaact cggtagcaga agaacttggt gatgttttat tcgttacgat gatcatggct
     1321 aattcattga atatcgactt aactgaagtt tttgaaaaaa acatggaaaa attcaatcaa
     1381 agagaccata atcgctttga gcgtaaggac gagtaaacaa gatgaagtgg tcctggcctt
     1441 gaagactctg aaacggactt gtcaaacagt caccatgcaa ctgagtcttt atctcaggac
     1501 aagaaacacg caggaaaggt tggaaaaatg aaattaaaac aattacctct ggaataccaa
     1561 aaggcaattc cagttctgaa aaaattagag aatgcaggat atgaagctta ttttgtaggt
     1621 ggaagtgttc gtgatatctt gctgaaccag ccaatccatg atgtggatat cgcaacaagc
     1681 gcatttccag aagaaatcaa acagcttttt ccaaaaacga tagatattgg gatcgaacat
     1741 ggaactgtgt tggtattaga aaatgatgaa caatatgaga tcaccacatt tcggacggaa
     1801 tcaacttatc aggactaccg tagaccagat catgtcgaat tcgttcgttc gttggcagaa
     1861 gatttgaaaa gaagagattt tacgatcaat gcgtttgctt tgaaagaaga tggagaaatc
     1921 atcgatttgt tcgatggtct gcaagatcta gaagaacaga tcttgcgagc tgtaggcaat
     1981 cctcatgagc ggttccatga agatgcctta cgaatgatgc gcgggttgcg ttttgtcagt
     2041 caactagggt tcaaattaga ggaagaaacc ttctacgcca tcaaagaaaa ccacgagcta
     2101 ttaggtaaaa tatctgtaga acgtataaat gtggaattca tcaagatgct cttgggaaat
     2161 gaacgggccg cgggaatcca tgcatttatt gaaacagagt gctacatttg ctgtcccggt
     2221 ttgaaagaag aaggcgaagc acttttacgt tttgctgaat taccatccgt cccattgaaa
     2281 gaagagagcc aagcatgggc attattgatc agccaactgg atctaaaaga aaatgagatt
     2341 cgttcctttt tgaaatcgtg gaagtgttct aaccaaatga ttaaagacgt tcaagcactt
     2401 gtccaaggac tacgacaacg agaaactgga gctttggaac cattcgaatt gtacttctta
     2461 ggcaaaaaac atgcactgca agtagaagaa ttgatgatct attttgacga aacacctcag
     2521 atggatgccg taggagaatc ctatgatcgt ttacctatca agtcattaag cgaattagca
     2581 gtagatggta aaatcttgct agaagaaact ggaaaaaaac ctggtaagtg ggttagtgaa
     2641 gctttgcaat tagctgaaaa agccgtcgta gaacaaagaa taaaaaatga taaaaaacag
     2701 gttttggatt ttttatcgaa agaaaaattg atttaaaaaa cactagactg tatctgctca
     2761 gctttgttgg ctacttaaag tgggcagata cgtcattaga agaaaaaaca taagatgtga
     2821 ccacttacat aaatttgtgc taaactgagg tagtacaaga ataaattaca tcataaagga
     2881 gtctttttaa tgagtaaaga aatgacagcg ctaaaatttt attttagaaa tggagaaacc
     2941 tggacgatca accgccgtca tatcggcgat ctttggatca aacaaatcac aacaagtttt
     3001 ggacgaatca atggcagtga atttgttgaa atccatccgt gtgctggatt taaaatcgaa
     3061 atcttccatg aaggggacgc agttgcgaca catgatatca acctaggcgg actggaaatg
     3121 ggcatgttta atcgcgcatt gaaatacgaa gatatcgagc gaatggaaat cctttaccga
     3181 aatggcacac ctgatcttgt gtacttccca tatctagata aaggaacaga aggattagac
     3241 aatcaatatc aatcaacaaa aatcagcgaa aaaacaggta acttgtatat cgtgatcaat
     3301 ccagatcaac gtgtagaaga tgtatacggg gaatttttcg aataaatcga tttttacgca
     3361 aaaatgaggc tgtgaaagag atgtgtctta ttactactga taggctgtct cttccacacc
     3421 gtttattcag aataagggac tgtgacaaga tttttgtcgc agccccattt ttttgttgtg
     3481 ataaaagaac aagaaaggca ggaatgggaa agtaaacatc cttttgtcag aaacttttat
     3541 ctttcttaag ttttacttga tatctgatac tatttggaat agaatgaggc gttgaaaaat
     3601 ttatggaaat ttctaactag caataaacga tgaaacaatg aaatattttt catccaaaaa
     3661 gaactttgct ataatgatgg aagtttgtgt ataggagatc tggaaaatga gagagagttt
     3721 tgaacaacaa aaaaaactgt tgcatgatcg ctatggcgcc ctttcgatgg atgaccgccg
     3781 tcaaatactt tgtaagttgc gtaaaagaaa tatattgatg tatcgtcagt tggaacgtct
     3841 gaaacatgat ctgcttcgtt tggagtcaaa acgtgtacag tgtgaattag aggggaacca
     3901 aacacaggtt gaagtagtcg aaaccaaaat tttaaagaaa aaagaacaat ttttaaaaat
     3961 gcttacacaa aataaaaaat aaaggggatg gacgatggaa tttgctgagt tagttattgt
     4021 atttgcagta acgatcacct tatcgaacat agctagccgc attttgccaa tgattccagc
     4081 tccactgatc caaattttcc ttggagtggt attaggtctg acagagtggg gacaatcaat
     4141 cgattttgaa cctgaattgt tcttagttat gattattgca ccattgttat ttagagaagg
     4201 agaaaaagca gatatttctt cgattctcaa aaatttcggg acaattttgt tcttggcatt
     4261 tggaggagtc attttgaccc ttgtcggggt aggtgcgacg ttatcattcc tgttgccaag
     4321 tgtcccactt gctgcctgct ttgcttttgg agcagcgtta ggtccgacag atgcggtagc
     4381 agtcagttct ctttctggac gagtgaatat ccctaaaaag gcgatgcata ttttggaagg
     4441 agaaggtctt ttgaatgatg catctggtgt tactgccttc cagtttgctc ttggtgcgct
     4501 gatcaccgga tctttttcag cagtaaacgc ggggatgagt ctagtagttt ctagtattgg
     4561 gggagctttg atcggctttt tgcttgtctg gttcaaacaa aaaataattc atttgatcga
     4621 aaaagcatca gcacaagatg tcacagggta tctgctgatc gaacttttgc ttccttttct
     4681 agcctatgta ttggcagagg catttgatgt atctggaatc attgctgcag tggcagcagg
     4741 tattctccaa gcttctggtt ttaggaaaat ctctgtgttt gatgcagaat tgtcgagctt
     4801 gtcccacagt acatggacaa ctattgcttt tacactgaat gcattagtat ttatattttt
     4861 aggaatcgaa ttgacgcaag tgttctcgcc agtatgggga gacggtttat atccaaacgg
     4921 tcttttatta gcgattatcg tattaatctc tgtcatgctt tttgtgatca gattcatttc
     4981 tatcagtctt ttttatgtat tcaaagatgg cagtaaaaag ttcaaaaaac agttgaacga
     5041 gatcttgatc ttaacatttg gcggtgtaaa gggaacagtc agtttggcta cgatttttat
     5101 tttgccattt tcaatcaata atatgatgtt ttaccagcgg tctttgcttt tgtttctgac
     5161 ggcgggtgtc atcttagtga ctttggtcat agggatcatt gtcttgccta tgctgacgga
     5221 aacagaggaa gcgaaaagca cggatttgaa tgctttgatg attttagaag aggtcgttga
     5281 agttttgcga aaagaaataa aggaaattga tcgtaataca aaagaatttt tagccactga
     5341 agcagtgata gaaaattatc aagaacggat acgtgatctg tacttggaag atttaaccga
     5401 tgatgaaaag caagaagtac aagaaataca agcactgatt ttgtcgatcg aacgggatgg
     5461 attggatgaa agttatcgtt cgggcaagct ttcttctaat ggttatcgtt tttactcccg
     5521 ttttctttct cgattcgaac attctatcac aagccagatt ttgtctttta tcggcttttg
     5581 gttcattgtt gtccgtaggt taatgcgaat cgttctccat ccgaaaatgt tttggcaaag
     5641 aagatatgct gatcgtcaga catttattag tgatgaagat atccaagaaa tcagagagac
     5701 atatcaaaaa aatactcagt tgattattga aagtttagat aacttgactg atatttatga
     5761 tgaaacgatg gttcaatttt ttatccagca acgcaaactt gaaggaataa aaatgaactc
     5821 ggggaatttg atttcttcgt ggatgatcca gcaagatagt ctgtttacga aaaaaatgct
     5881 aagaggttat tatcttgaaa gaaaaatcat tgatgaatac gaagttgctg aaaaaatcac
     5941 gacgttttct gcgaacagct atcgcaggaa tatcaatttg ttagaatctt atacgatgaa
     6001 caaaccatct gataactttt ctttccgatt tgcgtttcga gcaaagtcaa aaaaaacgag
     6061 tccaataaaa taaaaaggta aaaattttca agttttataa aaaacaaaac atatcagaac
     6121 aggatcgaat cggtcctgtt ctttttttga gaacacaaaa agaacaaata ccaaaaaaat
     6181 tttcctgaag ttcactattt ttatgataca atatccaaga taaggaggga acatgttgaa
     6241 agaattaaaa gtaactgacc tcaaaaaaac ttatggtgaa aaagatttgt ttgaccagat
     6301 ttcttttttg gtccacgaaa aagaccgtat cgggttgatt gggacgaacg ggacgggtaa
     6361 aacaagtctc ttaaatatta ttgccggtat cgatagcagc gacggtgatc ggcaaacagt
     6421 gttttatcca actgattacc gtattggtta tctttctcaa gcagctgaat tttccgatga
     6481 attaactgtt ctccaagcag tctttcaagg aaacagcccc ttgatccaaa ctgttcgagc
     6541 atatgaggaa gcattgatcg aactagggga aaacggagaa gaccctgatg tgcaaaaacg
     6601 ttatgctaaa gcagaagaac aaatgaataa agaagatgct tggactacag ataccaacgc
     6661 aaagattatt ttgcaaaaat taggaatcag tgaattagat aaaaaaatca gtactttatc
     6721 aggtggtcag aaaaaacgag ttagtctggc gcaagtgttg attgatgaac ccgatttact
     6781 attactagac gaaccaacaa accatttaga ttatgaagcg atcgagtggt tggaaaacta
     6841 tttgaaacaa taccgtggcg cactattgat ggttacccac gatcgttatt ttctagatag
     6901 agtagcaaac cgaatcttcg aactttcatt tggaaaatta tacgagtata aaggaaatta
     6961 tgaagcttac gtcttagaaa aagcagaacg tgatcgtgta gaagtagaac aagaagaaaa
     7021 gcgcaagcgg ctttataaac aggaattagc ttggatgagg gctggcgtac aggcacgtgg
     7081 caccaagcag caagcacgga tcaaccgttt cgaagatcta aaggagaact tgtaccaagt
     7141 aaaccaagaa gatgatttag agctgaacct agcgacacaa cgtttaggga aaaaagtact
     7201 ggaaatcaaa gatggcagtt accgtataaa tgaccaaaca ctgctagaac atttagattt
     7261 attgattcaa tctcgggaaa gactaggtat tactggtaaa aatggtgcgg gaaaatcgac
     7321 attgcttaat attctagctg gcagaattcc cctggatagc ggtacaatgt ctatcggtga
     7381 aacggttcat cttgcgtatt atacacaaga aaatgaagaa atggcaccag ataaacggat
     7441 gattgcttac ttacaagaag ctgctgaaga agcaaaaaca gcagatggca gtcaaatcgg
     7501 agtagcagaa ttacttgaac gatttttatt tccaagattt atgcatggga cactgatagg
     7561 caaactttca ggcggagaaa aacgccggtt atttttgttg aaactgctga ttcagcaacc
     7621 taatgtttta ttattggatg aaccgaccaa tgatttggac atagcaacat tgacgatttt
     7681 ggaagattat tttagaagtt ttccaggtgc tgtgatcact gtttcccatg accgttattt
     7741 cttggacaag gtagcagata agttattagt attccaagga aacggaaaac aagaacttta
     7801 ttacggaaac atgtccagtt atttactgaa gcaaaaagag acccagcagc ctgctgaaaa
     7861 agcgaaacca aaaatacaga gcaaggaacc tgctgaaaag aagaaattat cttacatgga
     7921 ac
//
LOCUS       DACCSK010000112         7914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCSK010000112 DACCSK010000000
VERSION     DACCSK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.115"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(90..1388)
                     /gene="eno"
                     /locus_tag="IVP39_002268"
     CDS             complement(90..1388)
                     /gene="eno"
                     /locus_tag="IVP39_002268"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7587326.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1504..2259)
                     /locus_tag="IVP39_002269"
     CDS             complement(1504..2259)
                     /locus_tag="IVP39_002269"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7587327.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2363..3553)
                     /locus_tag="IVP39_002270"
     CDS             complement(2363..3553)
                     /locus_tag="IVP39_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7587328.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3663..4664)
                     /gene="gap"
                     /locus_tag="IVP39_002271"
     CDS             complement(3663..4664)
                     /gene="gap"
                     /locus_tag="IVP39_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7587329.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4705..5742)
                     /locus_tag="IVP39_002272"
     CDS             complement(4705..5742)
                     /locus_tag="IVP39_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7587330.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(5945..7279)
                     /gene="rpoN"
                     /locus_tag="IVP39_002273"
     CDS             complement(5945..7279)
                     /gene="rpoN"
                     /locus_tag="IVP39_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7587331.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7339..7410
                     /locus_tag="IVP39_002274"
     tRNA            7339..7410
                     /locus_tag="IVP39_002274"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7372..7374,aa:Arg,seq:ccg)
     gene            complement(7602..>7914)
                     /locus_tag="IVP39_002275"
     CDS             complement(7602..>7914)
                     /locus_tag="IVP39_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7587332.1"
                     /translation="PPYGSVTNVGATIIQRPIIEWSVDSEDWKTRNPDLILQKIQATV
                     YDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLGHPTAVENYYGRNDHRPV
                     Q"
BASE COUNT         2059 a         1666 c         1464 g         2725 t
ORIGIN      
        1 ttcagcaagt agagagttcg attacttaac taagtgatca tactcgacta tctttcaata
       61 gtcgatcatg tatcttcatt agaaaagaat tatttgtttt ttaagttgta gaaagatttc
      121 aaacctttgt attctgcaac ttcaccaagt tggtcttcga tacgtagtaa ttggttgtat
      181 ttagcaatac ggtcagtacg tgatagagaa ccagttttga tttggccagc atttgttgca
      241 acagcgatat cagagattgt tgaatcttct gtttcaccag aacggtgaga tacaactgct
      301 gtgtagccag cttctttagc catttcgata gcttcgaaag tttctgttaa tgtaccgatt
      361 tggttcactt tgataaggat tgagttagca attccttttt cgataccttc agataatttt
      421 tgagtgtttg ttacgaacaa gtcgtcacca actaattgaa ctttgtcgcc taatacgtca
      481 gttaatttct tgaatccgtc ccagtcgttt tcgtctaatc catcttcgat tgagatgatt
      541 ggatatttag aaactaattc ttcgtagaat ttgatcattt cgtcagttgt tttttcgcct
      601 tcgcctgagt cagctagtac gtaaacacct ttttctttgt cgtagaattc tgaagaagca
      661 gcatccatag caagaacaac gtctttacca ggtacatagc cagctttttc gattgcttcg
      721 atgattactt caaaaccttc ttcgtttgaa ccaaggtttg gagcaaatcc accttcgtca
      781 ccaacagaag ttgctaatcc gcgagctttc aagattgaag ctaatgcgtg gaatacttca
      841 gcacccatac gtagagcttc tttgaatgtt ggagcgccaa caggcatgat catgaattct
      901 tggaagtcga ttgagttatc agcatgtgat ccgccgttga tgatgttcat cattggtgtt
      961 ggcaatactt ttgtgttgaa tccgcctagg tagtggtata aaggtacttc taggtagtca
     1021 gcagctgcac gtgcaacagc gatagaaaca cctaaaatag cgttcgcacc taatttacct
     1081 ttgttaggag ttccgtctaa agcgatcata gctttgtcga tagccatttg gtcacgaaca
     1141 tcgtagccaa tgattgcttc agcaatgatg ttgtttacgt tgtcgacagc tttagttaca
     1201 cctttaccgc cataacgagc tttgtctccg tcacgtaatt caactgcttc gtattcacca
     1261 gttgaagcac ctgaaggaac cattccgcgg ccaaaagctc cgctttctgt gtatacttct
     1321 acttcgattg ttgggttacc acgtgagtct aagatctcgc gagcgtaaac atcagtaata
     1381 attgacatgt tttgtctctc ctttgagttt gttttttcct aagggatttc cctcatatac
     1441 gattttagtt aattttgctc attagtgcaa acatttcttg catttttcgc aaattttcaa
     1501 caattatttt acagcgtcta acaatgacaa aaatgaatct gcttgcaagc ttgcgccacc
     1561 gactaaagca ccgtcaacat tttcttttgc catgtattca gcaatatttt ctggttttac
     1621 agaaccgccg tattggatac gaactgcttc tgatacttct tttccataaa gtttttcaac
     1681 tgtttgacgt acaactccac aaatttcgtc agcgatttgt gcgtcagctg atttaccagt
     1741 accgattgcc cagattggtt cataagcaat aaccatgttt gacacttgtt cagctgaaag
     1801 atcagctaaa ccagctgtaa tttgtccttc gatccattca gcagttttgc ctgcttcata
     1861 ggtttctaaa gattcgccgc agcataggat tggtgtcata ccatttgcaa agatagcttt
     1921 tgctttttta ttgatgtctt cgtctgtttc atggaaatat tcacgacgtt ctgaatgtcc
     1981 gatgatcaca taatgaacac ctagatcagc aagagctgct ggtgaagttt caccagtgaa
     2041 cgcacctgag ttttcccagt agcagttttg tgctgagatt tttagttctg taccttcagc
     2101 tgatgtcatt aattcttgta agaataatgc aggagatccg atgactgaat caactttgtc
     2161 atttgaaggg attttgtttt ttactgcttc agcaaattct tttgcttctg atgcagtttt
     2221 gttcattttc cagttacctg cgataatagg tttacgcatt gaggaacaca ctccttaatt
     2281 tattgaaaaa tggctaggga gtcatgaaaa ctcccttcac cttttgatcg attactcgtc
     2341 agaattgagc aaacttggtc gtttatttat cgttgattgc tgctagtcct ggtaattctt
     2401 taccttcaag caattccaag cttgcgccac cacctgttga gatgtgtgtg aatttgtcag
     2461 caaatcctaa ttgttctgct gctgcagctg agtctccgcc accgatgatt gttgttgcgc
     2521 cttctaagtt agcgattgct tcacaaacac cgattgtacc tttagcaaag ttactcattt
     2581 caaatacacc cataggtccg ttccaaacaa ctgtttttgc accttgtaat gtatcagcaa
     2641 ataattttac tgatgctggg ccgatgtcta aagccatcaa accatcagga atgtctcctt
     2701 cgattacttg tgtttcaaca tcatttgaaa attctggtgc acagacattg tcgattggca
     2761 agacaagttt gtcgcctgct ttttcgatta attcttttgc tagttccact ttatcagctt
     2821 ctactagaga gttaccgatc ttgattcctt ttgcttcgta gaatgtgtaa gtcattccgc
     2881 caccgatcaa gattttatct gctttaggaa tcaagttttc gattacgccg attttatcag
     2941 aaacttttgc gccaccaagg attgcaatca ttggtcgttt tggttcttct actgcttcac
     3001 cgatgaactt gatttctttt tccatcaaga atccagcaac tgttgggata ccagttgaag
     3061 cgatacctac gttagaagca tgtgcacggt gagctgtacc gaaagcatcg ttaacaaata
     3121 catcgcctaa tgaagcccag tatttaccta gttctgtatc atttttgctt tcttttttac
     3181 cgtcaatatc ttcaaatcgt gtattttcga atacgacaac gtcgccgtca ttcatgttgc
     3241 ggattgcttc ttctaattgt tctccgcgag tttcaggtac gaatgttact tctttaccta
     3301 ataattcgcc tagacgtttt gcaactggag ctaaagattt tccttcttta tcttcttctg
     3361 tttttacacg tccaaggtga gagaaaagga ttgcttttcc gccttgttct agaacgtagt
     3421 tgattgttgg caatgctgct ttgatacgag tgtcgtcagt gatcacgccg tctttcaaag
     3481 gaacgttgaa gtcaacacgg acaagaactt ttttgtcttt taaatcgata tctttcactg
     3541 ttttcttagc cattttattt cctcctgaat aaacttatta tcaaaaaaag cggaagaagc
     3601 gcgattgctt ccccgctttt aaatgtacat gtctataatt tacacttgta cgtttgtcta
     3661 tcttataagt tagcgaagta ttctaaagta cgtactaatt gtgcagtgta tgacatttcg
     3721 ttgtcgtacc aagcaacagt tttaactaat tgtttgtcgc caactgtcat tactttagtt
     3781 tgtgtagcat caaataatga accgtaagtc ataccaacga tatctgaaga aacgatttcg
     3841 tctgtgttgt atccgtaaga ttcgtttgaa gcttctttca ttgctgcatt gatttcgtca
     3901 acagttactt ctttttcaag aactgttact aattctgtta atgaacctgt tggtacagga
     3961 acacgttgag cagctccgtc taatttaccg ttcaattcag gaattactaa accgatagct
     4021 ttagcagcac ctgttgagtt aggaacgatg tttgcagcag cagcgcgtgc acggcggaag
     4081 tcacctttag gatgaggtcc gtctagagtc atttggtcac ctgtgtaagc gtggatagtt
     4141 gtcattaatc cttcaacaac accaaatttg tcgttcaatg ttttagccat aggagctaaa
     4201 cagttagtag tacatgaagc acctgagata actgtttctt taccagttaa tgtttcgtgg
     4261 tttgtgttat aaacgattgt tggaacatcg tttccgccag gagcagagat aacaacgcgt
     4321 ttagcaccag ctgttaaatg tttttcagct gctgatttag aagtgaagaa accagtacat
     4381 tctagaacga tatctacgcc tagttcgccc catggtaatt cttctgggtt gcggttagct
     4441 aatactttaa cttcttttcc gtttacgttg aatgaacctt catgaacttc aactgttccg
     4501 ttgaaacgtc cttgagttgt gtcgtatttt aacaagtgtg ccaacatttt agcatctgtc
     4561 aagtcgttga ttgctactac ttcgattcca tctacatctt ggatacgacg gaatgctaga
     4621 cgtccaatac gtccaaaacc attaatacct actttaactg tcatttaaga tttcctcctt
     4681 atgaaaatca aaaaaatatt tattttaaag ggttacccct tttaaaatct catttgcagc
     4741 agcttcatca gtgatgagcc acgtttgttt tggtgcattt ttcatataag ctcgaattgc
     4801 tttagctttt gattttccgc ccgcgattgc catgatgatc gggacttttt ccaaatcttg
     4861 caattctatg ccgattctag ggaccttata aacgacttca cctttttcat tgaaaaagta
     4921 gccaaaagat tccgcaacag catttgcttg tttcagcatg actaattctt tttcagtcat
     4981 cttacgacgt gctgccatgt gtaatgcacg cccaatacta tgaataacac aattcgcttc
     5041 actaatcaag tttaagactt cttggataga tggctcattg agtaaagaat tgtaagttgc
     5101 cagacttaat tgttccggaa cataaagtgc acggtggtta ccgtttgctt tagcagccat
     5161 tcttgcactg acagaatttg cctgtacagt tatcgcttca ccaatcccgc ctcttgcagg
     5221 gacaaacagg ttatgccgtt tcttattttc cagattagac aattgttcgg ctaccacagc
     5281 catagtagtt cctcccatca cagcgatgat attctcgcct tctggaagta gtcggtctag
     5341 ggcttcgctc agcgcttcgc cgaatgcatc agccactttg gtctgctctt cgctgtttcc
     5401 tgaaacaacg atacagcgtt ggttaccaaa atattctgcc aactgttgtt cggtttgatg
     5461 cgtacctaaa agctgatcca tgaagttttc aaggctttga taaacttctt cgcccttagt
     5521 tgtcagcgtc ataccgcttt ttgaggtgtc gatcaaattc agatttttca aaagatctgt
     5581 ttctgttcgc aatactcgtt ctgtgatccc catactttcc gataaacttc gacgtccaat
     5641 cggctgcatc caatagatat ttcgtaaaat tttatagcgt tcttgcaaca cttcgataat
     5701 atctggtgca acagattcaa tcatttttat ttcatccagc atagcgatct ccttagttgg
     5761 acactttacg tccaatgtag acttaaaacg acccatatac gataaaaaaa gaaattaact
     5821 attcgaattt cacatcaata aagggaaatc tcaacctcac gactgtaagt ctaacagttt
     5881 tgctctcgtt tttcaactaa aaagactaga aaaagtgaaa tactttttct agtcttttga
     5941 ctgattagtt atcataacgt tttctttttg atgaactcgg gattcctaat aaatcacgat
     6001 attttgctac tgttcgtctt gagatcgcta tctcatcttt tttcagcaat tcaaccaatt
     6061 tctggtcaga caacggcttg tttttgtctt cttgatcgac taattcctgc agctttttct
     6121 tcgctgtatc tgctgaaagg tcttctgtct gttctgtact attcgtagga attcgtgtag
     6181 tgaaaaattt ttttaattca aatacaccga agtctgtttc caaatatttc ccgttgactg
     6241 cacgactgac cgtggattca tgcacatcta tctcagttgc aacttccttt aacgtaagag
     6301 gcttgattgg acgttccttg tcgataaaga aagctttttg ccgggaaaca atgacttgag
     6361 caacccgcaa aattgtatcc ccacgttgta ggatcgtttt cttcaaccat tcgaattcct
     6421 gtttccgttc ttttaaataa ttttcagttt ctttatctgc ttgttgtgac atttgtttga
     6481 agtaactctc ttgaaagcgt atatttggct gattttttcg gtttgagata acttgtacat
     6541 ttttatcgtc gtccactaat acccggacat caggaataat gtacaattcg ctgctacggt
     6601 cgaaaatccg gccagggctt ggactcaacg tttgtaaata gtcgaatatc tgctgaacag
     6661 catgtagatc aacagaaaaa cgttgtgcga tttctttcca ttttcgctcc accagttcct
     6721 caaaacattc ttccaacacg atataagcta attccggcgc ataatcatct cgttctgttt
     6781 gaagcatgag acattcttgc aaactacgtg cacctactcc agctggttct aattgctgaa
     6841 tcagagtcaa cgcatcgagc atttggatag gcgtagctcc aatctctttt ccggcttctt
     6901 ctaaactaat cgtcaaaaat ccgttcaaat cgatgtattc tacaagataa agaacgattt
     6961 tcctcaaaaa agtatctcgg taattcaaat ggacttgttc gatcaatgaa tcaaataaag
     7021 acccttttgt atcaggaatc tgatttaggt aatttgtctc ttctccttca taagaagatg
     7081 aagaagcttt tatgtaatct ggctgccatt ccggctcaac aacttccacc aatggatttt
     7141 ccattgcttg attctcaacg aatgccaaca gctcctcact gttatattgt aaaacttgga
     7201 tggactgttg caactgttgc gtcatggcta gtttctgtgt ctgctgctgt ttttgtgaaa
     7261 aattttgctc aaatttcatt tttttgtcca atccccttgt aattttttat ctgtttagtt
     7321 acaattatcc cagtgcttgc gtccttagtg tagtggatat cacgtaagat tccggttctt
     7381 gagacggggg ttcgattccc tcaggacgcg ttctactgct attttagcat aatttacgaa
     7441 tagttgcttc tttattttta agtgaaaact gtccgccaaa gaaaattggt ggacagtttt
     7501 ttgttgtacc tgtatagata ttgttaagcc tttgcaagta aggtagttat gtgttcaact
     7561 acttattaaa atgtgttatt tattaaagat acttgtctca cttactgaac tggccgatga
     7621 tcgtttctgc cataatagtt ctcgacagcc gttggatgcc ctaataaatc cccaactgtc
     7681 gtgatgcgat acccttgctc tttcagataa tcgatcactt gaggaacggc acgaatcgtt
     7741 tcaggataga tatcatggaa taaaataatt gccccatcgt atactgttgc ttggatcttc
     7801 tgcaaaatca aatcaggatt tcttgtcttc caatcttctg aatcaacaga ccattcgatg
     7861 atcggtcgct ggatgatcgt tgctcctaca ttcgtgacag aaccataggg cgga
//
LOCUS       DACCSK010000113         7778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCSK010000113 DACCSK010000000
VERSION     DACCSK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.116"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(144..1013)
                     /locus_tag="IVP39_002276"
     CDS             complement(144..1013)
                     /locus_tag="IVP39_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7587333.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1033..2154)
                     /locus_tag="IVP39_002277"
     CDS             complement(1033..2154)
                     /locus_tag="IVP39_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7587334.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2156..2551)
                     /locus_tag="IVP39_002278"
     CDS             complement(2156..2551)
                     /locus_tag="IVP39_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7587335.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2570..3367)
                     /locus_tag="IVP39_002279"
     CDS             complement(2570..3367)
                     /locus_tag="IVP39_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7587336.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3369..4157)
                     /locus_tag="IVP39_002280"
     CDS             complement(3369..4157)
                     /locus_tag="IVP39_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7587337.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4171..4647)
                     /locus_tag="IVP39_002281"
     CDS             complement(4171..4647)
                     /locus_tag="IVP39_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7587338.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4719..7376)
                     /locus_tag="IVP39_002282"
     CDS             complement(4719..7376)
                     /locus_tag="IVP39_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7587339.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(7395..>7778)
                     /locus_tag="IVP39_002283"
     CDS             complement(7395..>7778)
                     /locus_tag="IVP39_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAP7587340.1"
                     /translation="KLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLEQVVLLAD
                     ESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQAT
                     FQLNDLNKAFLASHIYGKILSAKFE"
BASE COUNT         2454 a         1597 c         1103 g         2624 t
ORIGIN      
        1 ggataatttt tctcttaaaa aatatcatta tttgtggtat ggagctgggt atcatgagtg
       61 aatgatattg ctgaatacaa atagtccgac cacattaagt tgatcggact tcataagcat
      121 tttattgact aatacttttt tgcttaagaa ttgattctaa catcttttgc taaagttaag
      181 catcctatag ttcctgcatt atctgctaac ttaggtgtga caatgtattc ttcgatattt
      241 ggcgtatcga catagccgtt aattagttct aaaaaacgtc tttgtacttt ctttttcaga
      301 tgttcttgtt gcatgacacc accaccaaaa attataatat ctggtgaaag catcagggtc
      361 gtattacagg cacattgtgc aatataatcc gcctcgattt cccagaacgg atcttctata
      421 gaaagatctt gtcccttggt tcccgtacgc atctcgatag cgggtccaga tgccaaccct
      481 tcgagacaat catgatgaaa aggacaagta ccagagaaag tatctcctga atgtctctta
      541 actaacgtat gccccatttc tggatggcta aacccttcaa caaaaatccc atcttgtata
      601 gctcctccac caattccagt tccaacagtg taataaacaa cactggataa accttttcca
      661 tgacctgaaa catactcccc gtaacaagca gcattcacat cggttgtcca ggcaattgga
      721 atttcgaaat gcttattcaa cgttccaaca aaatcaaaat ttttccaggc agtttttgga
      781 gtagaggtga tatgtccata ggtctttgat tcacgatgga tatcaatcgg tccaaatgac
      841 cctactccaa tgctttctaa ttgttcttta tagtgattaa agaaatcaat taccaaactc
      901 attgtttcat tcggtgtagt tgtaggaaaa ctcactcttt ctaaaacctt taaatcatca
      961 ttgccaatgg cacaaacaaa tttagtccca cctgcttcta ttgagccata catttcttac
     1021 tccaccgtcc ttttatctag gaattcgttc ttccctgtct aatagttttt cgaaaaactc
     1081 atgtggttcc ttttggtacc aataggcaac agaagaaata tcgtcttgac gctcataaag
     1141 cttgatgtca tcatttccta gggtttggaa agtaacacgt aaattttctt tgaaactaat
     1201 ggggtcgggt aaatgccagc ggtacaaccc atgcatcggt aaagcatcat cgccaaaacc
     1261 atgaactgga tttggattag gttttccagc actaaaataa tcccgggtgt gatctctttt
     1321 agattgaaaa ggatagccca taaatagcgt attaaaattt tgagcatatg ccctttctcc
     1381 aaagttcttt ttatggaaag cccaagcgcc gccaaaataa tcttctgctc ctgtagatgt
     1441 tattgtaggg aaatcttcat ctccatctat ataaaactta aattctcctt ccccccacca
     1501 gtatctttcc aatgcgcaca aagcaaagta ggttcctaca taatacccag tgcctttgat
     1561 tccatcaata agggtgtaat cttttccttt tgtcgtgatg ggttcacggt tccatttagc
     1621 atgaaaatac atcgcatcat ccggtacttc agtcaatgca taattaaatg tataaaaaat
     1681 gtgcttaata aactcaggat gttcattcgt taaagtaatc ttagcttttt ttcgaaatgg
     1741 catctcaaaa tagcaattca tccctgatgt tggattcact acgattggta aggaattcac
     1801 ttcgcatctt gtcccaaacc cattacaaaa aaaatctcct aatggacttt ctacagaagg
     1861 agtggattca tcatcccaat aaatacgcaa aattaaatca cgtaagacga aactcccagc
     1921 gattgttttg tctgtcactg tcatccaaaa atgacgtaaa attccagggc cttccatttc
     1981 tgctaaagta attgtctctc cagcatttat cggaacagaa ggtgaccctt ttctcccaat
     2041 tcctagatga ctcgctgtca ttgccgcttt ccctttttca ccatttttat tttctggtga
     2101 aatagttcta ctttcctctc ccttgtggag ccatatatta tttaaacaat tcattttatt
     2161 cgtcctctat cctttcttca cttttttgat tattcatata gatgatttgt tgttgtgcct
     2221 ccgcaataat tgctccaata tctaaattat catttcctaa ctgcaaagcg attgacattg
     2281 ccaagggaag attcattcct gcaattacgt gcactctgtc agaaatatag gtaaaaaact
     2341 tttgatttac acttcctcca gccatgtcag ttaaaacaat tatttcgtct gtagtatcca
     2401 ctaactcttt aatttgttca tctatatcat caatttcttc gacatatgca cataagctac
     2461 taatttgatt atgcatacct gttaaaaact ctagtgtctc tttaaaaccc tcagctagta
     2521 ttgaatggct agcaatgatc aatcttctca taattcaaat ccttttcctt tacgctaata
     2581 ttccaaatgc agccataacc atcgcaaaaa caataacgag tagaatcaaa atggtcatac
     2641 tgattttttt cttactgagt agataatatg ctgcagcagt aataattact ggaattaatg
     2701 ttggtaaaat tttgtccaac attggttgga ttttcatact aacttcacca aaagtgaatt
     2761 taagtggcgt tgaaacactg ataactgaag caattaatga tccaactact gttagcccca
     2821 atactgaagc agcttcagtt aaataattaa tcttttcacc gtattcagta acaatcttca
     2881 ttccctgctt ataaccaaat tcaaagcctc tcattcggaa aaagaagatt accatgatta
     2941 aaatgaacca taaaaagatt cctacaggat tattttctaa agccatataa gccgcaattg
     3001 atcccatgat ggttgggatc atcgtcatca aaagagagtc cccaatacca gcaattggac
     3061 ccatcgtacc aactttaaag tcattaatcg catcttttgc ttctaatcct tgcttttcct
     3121 ccattgcaac acaagcacct aaaataatgg ctgccagcca aggctgtgta ttgtaatact
     3181 gaaagtgatt atttaaagct tcgatgtatt catcatcatc tttgtataat tttcttaaaa
     3241 taggtgacaa tgcgtaagtc acagatgcac cttgctgtgt ttgataatta aaggtacaaa
     3301 ctgacatcaa ccaacgatag ttcgctttcc taagatcctt tttcgttact tgatattttt
     3361 cgctcatttt attcatcctc cccaataaac ccaacatcag aagctcccgc agaagcagtg
     3421 atttgtgcat ctttttcagc tctatgatac acattaaatg ctaaagcgaa tcctatgatc
     3481 gccgctccaa taataggtac cttcaaaaaa gcggctaaga aaaatccaac aagtaaataa
     3541 ctaatgtatc gacctgtagg cataaagcgt aacaacatcg caataccaac agcaggcaac
     3601 atttttccgg ctaatgttag cccatccata aaccaattag gtaaaacatc caaaacgtaa
     3661 ttaacagcag actgtcctaa agtaacggca ataaatactg gaattgctgc agataaacca
     3721 aagaaaattg gccccaacca caaaacacta ttcattttct taaattctcg tttattcgca
     3781 tattcctgtg atttgcgaac aacaaaacca tttaagattt tgacaattac atctagttgc
     3841 acccctaaca tacctacagg tagtcctatt gctaatccgg cttctactcc aagctggcta
     3901 ccaaatgccg ttgcaataat tgcgcacgtt ccatagtccg gtacagatga gccaccaaaa
     3961 ccggcaactc ctaatgacat caattgaact gtaccgccaa ctatcaaacc tgtttttata
     4021 tcacccatca aaattcctgt gaataatccc caaaaaactg cgtaatttac aaagatcatc
     4081 ggaatcccat aataatcaac atgtttaata aatgctaata tagtcagcat aataatttga
     4141 aaaatagtaa aatgcattat ccttactcct ttatcctaat tttgattgaa tttcctgtgc
     4201 cttatccgcc ggaacaaatt gtgtaacaat ttctacacca gaatccaaca attttttata
     4261 cgtgctgata ttttcatctg atgcataggc tctagaagat agctgtaatg cattatcttt
     4321 aacaacagta ccgccaacaa ttagtcggtc aattctaata ccgttatcta ctaaatgaag
     4381 gattgtttcc ggctctttca cgatgacaaa tactttttcc tgatcgtatt tccccttttt
     4441 aaaattcgca atcgctgttt cttcagtgat aattgataca gccattccag caggtctagc
     4501 taaacgcata cttgattttt ggatgtcgtt atttgcgaca gaatcatcga tcaccatcac
     4561 tctgtttgct ttagacattg gtgcccattg cgtgacaata atcccatgca ataaacggtt
     4621 atcaactctc gacataacaa tactcataaa tacttcctcc tttatctttc attttcttac
     4681 aagcaattac catgccaaaa caaaaagaac ttgataaatc aagctctttt tacataatca
     4741 taaacgtatt taacctcatg aatcgggatg tcgacactat aataatgttc aacgacactg
     4801 aaagattgct taaaatgtgt ctgaaatttt tgaaattcct cactcgtttc attgaaataa
     4861 atttcatctt ccaaagtatt ttgttttact aatcgttcaa ttaagcaact aatgtgaaca
     4921 tataatccga aacacgtatt attatcaaat gaaattccta gatcattttg taagatgtat
     4981 atagcatctg aaacccgttc taataattta gtcggattta atatggtcaa ttgctccatt
     5041 aaattcgtta gactaaaatt cttcagaata ttattgttca aaactttcag ttcattatca
     5101 ttaaagtgtc tattcaattg attggagaat ttacttatat tcattcctaa aatcatatct
     5161 tcaattgaaa agaattctac atcttctaga tgtggattta gtgttccaac aataaaaaga
     5221 atattgttct ctttacgaag ctccgataaa tagttttcat tcactaaaga gtaatagtca
     5281 cacgtttcta tgacaagatc agtatccctt ggaaagctat cttcaagtat cttttttaat
     5341 ttaaacgaag ttcctaatcc gcttgcacaa gaacagacaa tcatatcctt cttaatattt
     5401 gatgattcat aaatataatc cacattttga ttttctttta cttgttcaag aatctgttta
     5461 agcggtaaat ctaataatat ctgatttgca acatcaatag ccattcttgt tgaaacattg
     5521 tttagtagtc cgaatttttt ttgagcgtct agttccaaat ttagataaat tttctccagt
     5581 gaacccatat caacaagtaa aataatttct tcatatccgg tacactcttg taagtatcta
     5641 ttaattttgt ctgcaatctc actacttgaa gtcattagtg gcatatcaat ggcgtcgtat
     5701 atatatctct caacgatcct attagcagta cttgctatac aggttgccgt tagatctcca
     5761 tgagccaaaa tcagtgctaa acgtttttgc tgaaattctt tactacacat taacttaaat
     5821 aaaataacca aaacattttt ttgaattttt gttaaataaa tatcccctat ttgaataaaa
     5881 cgattgacta attgaacttc tcgactacac caactattaa tatcattgat gaaggaatat
     5941 aatttatcgt ccgatgttaa ttggatttga taaatccaag aattcaaaaa gatagacgtt
     6001 atttgaagat aataatagtc aaattctatt tgaaaatctc tctctatttc cttaacatac
     6061 tgtctaaatc gaatctcttg ataggtggga tatgttaaag ctgaattatt ttggatatca
     6121 acattctttt gtctaaaata tctatcatat ttctcaaaaa gctgctgctt atcagtgtct
     6181 ttcttccacg tttgataaag atactctata agttcgttga tattaaaaac ttcgtctact
     6241 ttttccaaat catccatttg aatatatttt aattcattct gtcttttcat aaccttcatg
     6301 ccgttgaaat tcacccattg tggtaaatga tacatctgaa tataaagttc ttccaaatca
     6361 tctaagaaat aaaaggcatt ggcacaggtc aattgaatga tatttttcaa attgccaaca
     6421 ttttcactaa agcgatagtt taataaaaaa tctaataatt gttgagatat cagtattttc
     6481 tttgctagtc tttcttcttc tgatttaaaa aatatcttga ttaactgttt tctttcataa
     6541 agagaacgac tgtctaatgt tggaagtgtt agctttacag gtattcttct caacaaagtt
     6601 gataatagtt tatcctcagg tggttctgat gttgcaaata taatccagca acttgatgtg
     6661 taccagtttt cattatcacc catccgatga aatttcctcg tatccataaa ctgaaaaagt
     6721 ttctcctgac attcattgct caatgcatga atttcatcta aaaataagac tccattatta
     6781 gctaattctg ctaaaccctt tcttccatct tcggctcctg tatatgcacc ttttacatgc
     6841 ccaaaaaaat tggacaagaa aagttctgga ttattggcat attcactaca attaactgaa
     6901 atgaaagatg ccttctcttc tatataatta ttggccaaca aatccttata cacataattt
     6961 gctaaatagc tttttccagt tccagtctcg ccatgaagta aaatgggtaa gccatttggc
     7021 ggatagtaga ttgctgcttt aatttgagca attacatttc gcagacttcc ttgacttccg
     7081 ataacccttt ctattccaac cctaaaagag ctatcatacc atttttccgc ttcctctgaa
     7141 gagctaaaaa tttgttgatt tggaactttc tccggtaaag aaaagaagat tactggacga
     7201 gtattgattt ttccaaattc tccatctttt acgaattcat tcaaatattg agagacaata
     7261 ttacgactaa catttaattt ttcagatatt ttctgtgctg tattgttccc ccatttattt
     7321 ttaatctcag agttctctat gacataattg acaatgcgtt cttttaattc ttccatcaca
     7381 gcacctcctc gacactattc gaattttgcc gacagtattt ttccataaat atgacttgct
     7441 aaaaaggctt tgtttagatc attcaattgg aacgttgctt gttttgcttg atccgctaag
     7501 acgttgaaaa gatactctgg cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc
     7561 agatcttctt ttgattcttc atctgaaagt aattcacgat acaacatcgt ttgtttgctt
     7621 actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg
     7681 tcggataaat atttatagaa aatcagcccc aataaataat ttttatattc tgacgcatcc
     7741 attttactac ggagattatc tgccgcacta aataactt
//
LOCUS       DACCSK010000114         7658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCSK010000114 DACCSK010000000
VERSION     DACCSK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.117"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..803)
                     /locus_tag="IVP39_002284"
     CDS             complement(<1..803)
                     /locus_tag="IVP39_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7587341.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKS"
     gene            1061..3850
                     /locus_tag="IVP39_002285"
     CDS             1061..3850
                     /locus_tag="IVP39_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7587342.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQKLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDCGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3897..4424
                     /locus_tag="IVP39_002286"
     CDS             3897..4424
                     /locus_tag="IVP39_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7587343.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4445..5635
                     /locus_tag="IVP39_002287"
     CDS             4445..5635
                     /locus_tag="IVP39_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7587344.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRISYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5675..6973
                     /gene="asnS"
                     /locus_tag="IVP39_002288"
     CDS             5675..6973
                     /gene="asnS"
                     /locus_tag="IVP39_002288"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7587345.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2521 a         1419 c         1646 g         2072 t
ORIGIN      
        1 aagcttttca tgattcgtac gcgaatatcc tcaaatttac gaatgagatg tttgacaatg
       61 tgaaaacgat ccacaacgag ttttgccttt ggaaaacatt cctttatcag agaggcataa
      121 gaaacattca tgtccgtcac gatgaccttt accttcaatc gtgcgcgtcg ttcaaattgc
      181 ataaaataag aaacgagagc ctttttcttc cgtgaaggaa gaatatccag taattttcca
      241 gtatcgccat ccatacaaga aaaggagtag ggaccgactt gatttttcag tgaacgaaat
      301 tcatccatta ataaacaacg cggtaaccaa tttcttttta cttttgtcat ggggatcaga
      361 gaagcgagtg tccgagaaac ggtcgaccaa gaaacgccgc aatgcttagc aattgtcgtc
      421 attgcttgtt tttcagttag ctcttctaaa attttatagc gcacttgttt agcgataaag
      481 cagtttctat cgacaatgta ggtattcgcc gtaaaatagg tgtggcagcc acgacagtgg
      541 tagcgttgtt tcgctaaagc aagataagtc agttggttcg cacatttatt aagttgaatg
      601 agcgattttt tctttccgtt cttaacgatc atgccttcat gattcatctg tttacacaaa
      661 ggacagcgac gaatttcttt acttaacgtc gcagaaacaa caagactctt gatattctta
      721 atgctcttcg attccacatt tgtgatgaaa atgtttttat ctttgattcc aagtagtagt
      781 ttgatatgat gaagatcagg catacagaac gctcctttga ttgtgttgtg gtgattcaat
      841 tctaacggag cttctgtatg ttttgttttt tcatgctcaa aagggaccga tagaatgatt
      901 ttctatcagt cctagaaatt atagagccaa aataaacgcc cagacatttg cgtgtctggg
      961 cgtttttgtc tattttcttt aaaaagcgat gagagtattt ttctgcactt tgtagtataa
     1021 tttcaaaaga acattagagg caggagaaga ggaagatatt ttgaaaccaa gaacttatgc
     1081 agtcgttgat atcgaaacga ctgggacgaa tccaaaagaa gatcggatca tccaatttgg
     1141 ctgcgtcatg attgaatcag gtcggatcac ttcacgtttt tcgatcgata ttcatccagg
     1201 gcggaagatt tcaaaacaaa tccagcattt aacagggatc acgaatcaac gcgtccaaaa
     1261 agcgccttat ttcgaagatg tcgcccaaac gatttacaat cttttggcag acactatatt
     1321 tgtcgcgcat aatatttatt ttgattataa ttttttgaac catgaactca tgcgatgcgg
     1381 acttcctagt ttgaagatac caggcatcga tacagtagaa cttgcacaaa ttttcttgcc
     1441 gacggaacca agctttcgct tagctgattt gtcagaaagc cttggattta ttcatgagaa
     1501 tcctcatcag gcagatagtg atgccgaagt gacaggtcaa cttcttttgc tgatcgaaga
     1561 acggatgaga aaattgccaa tcatcacgtt agagcagatt gcgcgattaa gtagacacac
     1621 aggtatggat acgagtcgtt tcatttatca tgtgattgaa gaaatgaaag aaaaaccaga
     1681 gccacttgat ccctcattag aaatcgtcga tgggctagct ttgcagaaaa aagaagtgga
     1741 actattcaca tccgtccatt atggcgagcg gacatatcca agaaaaaaac aagcaaaaga
     1801 aaaaatgttt ggtaaaacac tgatgtatcg aaaagaacag aacagactga tgaatgcggt
     1861 ctacgaccat ttcacaaaag atgaatcaaa agatttgatg atcgaagcag ctacaggaat
     1921 gggtaaaaca atcggttatc tgctgccatt gagttatttg gctacacctg aaaaacccgc
     1981 tgtcatcagt accgtttcgc tagttttgca gcagcagatc atagaaaaag acattccatt
     2041 gctgaatcaa ttgctagatc agcccataca acctgtaatt atcaaaagtt atcgccacta
     2101 tatcgacctg caacgattta aaggaacact tgtggaacca ccagaacaaa aacaatatgc
     2161 tttgtatcaa atggcggtct tagtttggct gacgcaaacg aagaccggtg atttggatga
     2221 attacattta acaaacttga accatgcatt ctttgcagat atcgcgcaca gaggtactgg
     2281 gtttttagca agaaatcagt cgttttacga acaagacttt gttcgtttct tgcaaaagaa
     2341 aatcaggcaa agtaattttt tgattgtcaa tcatgcgttt ttagtacaag aaacacagcg
     2401 aaaagaacca ttgcttccag ctagtccatt tttgttgatc gatgaagtac atcatttgcc
     2461 ggaaatcgct gaaaaagtaa atgacaaacg attaagtatt aactatttcc ataaacaaat
     2521 ccagcaatta gaagaaccaa atcagctgtt tgaccgaata caaagctatt taccgaaaga
     2581 acatgaagcc ttacgctggt tatcgattta tcgagaagaa ctttctgcgc tatgcgaagc
     2641 acagatttgt atggcagaag gattaatgag tttagtgagc ggcaagcaac ccaacggaca
     2701 gtatccagaa gaagtcatga tgacaaaaga gatgttggat cagctgccac tggaatctgg
     2761 gacagccatt gatcacctgc agctttttta tcaggagatc cagaagctac agtcacaaat
     2821 gcgtctgact tttgagatta tccaagaaag ctggacaaac cagatgcgga tcgatttagc
     2881 aagtttatat agcttatttg atcaattaga caagcagtac ttgctgatgg atcaatggct
     2941 ggaagactgg cgagatcatt tgatccattg gttcgttcct gggaacacag cgcagcagat
     3001 gatttttcat ctccatgatt ttcaagcggc tatgttgccg tcgactgttt ggtatcctcg
     3061 atatgatcgt attctttata ttggaggaac gctgaagatc ggccctaatc gacactatct
     3121 ctctaagcgg cttggcattg aagcagcacc tcttaaagtg attgcctctc cttatgatta
     3181 tgagaaacag gcaaggatct atgtgccaaa agaggcacct gccgtccatc aagtcagcaa
     3241 tcaagcatat atcgattatt tagagacagc acttacaggt ctgatcgaac aagaagatcg
     3301 gccaatactt gtgttattca cttctcatga tgttttgcag aaggtctatc agaaaatgca
     3361 tctgtctatg ctagaaaaag gacgagaaat actagctcaa gggtttggcg gaagccggga
     3421 gaagcttttg aaacgcttca ttttgtcaga aaaaagcgta ttgtttggcg ctgacagctt
     3481 ttgggaaggt gtagatttgc ctggagatgc tttgcagatc gttgtagtca cacgtttacc
     3541 cttcgataat ccgcttcgcc cgatggtcaa agctaggaac gcttatttag aatcagaggg
     3601 gatcaatcct ttttaccagg aatccgtacc taaagcagct ttgaagttac gccaagcatt
     3661 aggtcgcttg ataaggacgg agacagattg tggcgtattg cttattttgg atcgaagact
     3721 agtcactgca aaatatggtg cacggatcat ccatgcatta ccgaaaaaag taccgatcaa
     3781 agagctagct atggaggaaa tctatcagga catccatgaa ttcttaaaga aaaatgaaga
     3841 aggaagttag aggagctgtc ttttttcata attaaaatct gctataatga aaaggaatga
     3901 agataaagga gcgacgaccc ttgaagaaaa aacagacaaa ggaaaaaacg attaatcgcg
     3961 tgttacttgg cttgatcgta tttttattag cggtcatcgt gatcagctcg attttttact
     4021 tgcgttccag tcgtccggcg gtgcaggcga agaaagaagc agtagaaatc gcaaagaaat
     4081 atgcaaatct tgaggaagcc gatcattttt attggtttac tcgaaagaag acatactttt
     4141 cactgacagg tacaaatgat aaaggaaaag aaatcgccgt tatcattcct aaatctggtg
     4201 aaaaagtaaa agtattggat caaacaaagg gactgactga aaacgaagca aaacagacaa
     4261 ttgctgcggc ccatcctgaa atccaaatcg aaaaagctgc tttagggatg tatgatgatc
     4321 agcctgtgtg ggaagtagta ggaaaagaaa caaatgggaa actgaattat tacctgattg
     4381 gctttgaatc tggagatgaa gtcaagacaa tcaagggaat ttaaaaggta aaggaatgaa
     4441 tgggatggag ttatcaaaaa gaacaaaacg attagaaccg tctgtcactt tggcagctgc
     4501 ggcaaaggcc aaggcattaa aagcaaaagg cagagatgtg ctgagtttga ctgtgggaga
     4561 accggatttt gcgacaccag aaaacatcca agaagcagca atcgaagcaa tccgcaatgg
     4621 gaaagctagc tattatacac caacagcagg aatcccagag cttcgacagg caatcgttga
     4681 ttatctgaag aaaaactatg gacttgtcta tgaaccttca caaacagtcg taacagacgg
     4741 agcgaaattt gctttgtata cccttttcca aacgatcctt gacccgcaag atgaagtcat
     4801 cattcctgtg ccttactggg tcagttacgg tgagcaagta aaactggcag aaggacttcc
     4861 tgtctttgtg aaaggggaag aatccaatag ctggaaagtt actgttgaac agctagaaca
     4921 agcacgttca gaaaaaacaa aagccgtaat catcaattct ccatcgaatc cgactggaat
     4981 gatttatagc aagaatgaac ttcaggcaat cggtgaatgg gcagtaaaaa atgatatttt
     5041 gatagttgct gatgatattt atggtcgttt agtttataat ggcaatgagt ttactccaat
     5101 tgcaacgatt tcagaagcaa tcaaaaatca gaccatcatc atcaatggcg tgtctaaaac
     5161 atatgcgatg acaggctggc gcattggtta tgcggtgggg aattctgaga tcatcaatgg
     5221 aatgattgct gttgcttccc agtcaaccag taatccgaca gcagtcagtc aatatgcagc
     5281 tgttgaagca ttgaaaggtg agcaagatac agtagaagaa atgcggaacg cctttgaaga
     5341 acgcctgaat acgttatatc cgcttgtggc agaactgcca ggtgtttctc ttgaaaagcc
     5401 gcaaggagct ttttatcttt ttcctaatgt aaaagaaacg ctacgcatgt gtaaatatga
     5461 aaatgtcaca caatgggtag aagatctttt agaagaaaca ggggtagcgc ttgtgactgg
     5521 cgaaggcttt ggggcaccag aaaatgtccg cataagttat gctactgatc gcgctacgtt
     5581 agaagaagcg gtacgcagaa tcaagcaatt tattgagagt aaaagtcaaa attaatcctg
     5641 aagaaattca aggaatataa aaggagagtc atacgtggaa caaattcaaa tcatcgattc
     5701 gaaaaaccat gtgggagaaa cagtaacaat cggcgcttgg gtcgcaaata agcgatcaag
     5761 cggaaaaatc gcatttttac aattaagaga cggaacagcc tttttccaag gtgtagttgt
     5821 aaaaagcgaa gtgtcggaag aagttttcca aacagctaaa aatctacacc aagaaacatc
     5881 cgtgttagta actggagaaa tccgtgaaga cagtcgttca aaatttggtt atgaaatcgg
     5941 aattacaaat gtggaagtgg taagcgagag ccacgattat ccgatcacac caaaagaaca
     6001 cggaacagac ttcttgatgg atcatcgtca tttatggctg cgttcttcaa gacaacatgc
     6061 gattatgcag atcagaaatg aaatcattcg tgcaacgtat gaattcttta atcaacatca
     6121 ttttgtcaaa attgatccgc cgatcttgac tggttctgca ccagaaggca ctacggatct
     6181 attcgaaaca aattactttg atcaaaaagc ttatttatct caaagtggcc aattatacat
     6241 ggaagctgct gctatggctt tcggaaaagt cttttcattc ggtccgactt tccgtgctga
     6301 aaaatcaaaa acacgacgtc atttgatcga attctggatg atcgaaccag aaatggcttt
     6361 cactcatcaa gaagaaagtt tagaagtaca ggaacaatat gtagcattct tagtacaaaa
     6421 tgttttagat cattgcgaat acgcattaga tgtactagga cgagacaaag aagtcttaaa
     6481 aacttataca aaattgccat tccctcgtat tacttacgat gatgcaatcg aattactgaa
     6541 gaaaaatgga tttgatgaca tcgaatgggg cgaagacttt ggttcacctc atgaaacgtt
     6601 cattgcgaat tcattcgaac agcctgtctt tatcctaaac tatccaaaag ccatcaagcc
     6661 attctacatg aaaccacatc caacaagaga agacgtggta atttgtgcag atatgatcgc
     6721 accagaaggt tacggtgaaa ttattggcgg aagcgaacgg gcaacggact atgatttctt
     6781 gctagaacaa atccgtaatc acggtttgga tgaaaaagaa tattcatggt atcttgatct
     6841 acgtaaatac ggttctgttc cgcactctgg ttttggtcta ggtcttgaac gaacagtcac
     6901 ttggattgct ggtatcgacc atgtacgtga agctattcca ttcccacgtt tgttgaaccg
     6961 aatttatcca taagaggttg tgcgtctgcc gttcagctcc gagtcacaag gagtaatttc
     7021 aaaaaacagt ttttcatgtt tgatgattat tgtgacttgt gaccaaggaa ttggccggag
     7081 agcaacgttt atttaaaaaa aagaactgcg aactctcgca gttctttttt ttaaatacgg
     7141 gataaaggaa gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac
     7201 aaggttctat aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga
     7261 aatctaattt gatgatgctt ctgctgttga aataaagcag catctagcac ctgaaaacaa
     7321 actaatagta acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat
     7381 gtaagttcaa ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca
     7441 agcaatctag aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata
     7501 aatatatgtt tcgatggcgt catcagtggt ctgattgacg gaagcttggg tgtgatcgat
     7561 ctctctggac caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc
     7621 ataaaaaact agacacttga tgagcgttca ttcaattg
//
LOCUS       DACCSK010000115         7594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCSK010000115 DACCSK010000000
VERSION     DACCSK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.118"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            259..1797
                     /locus_tag="IVP39_002289"
     CDS             259..1797
                     /locus_tag="IVP39_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7587346.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1794..2759
                     /locus_tag="IVP39_002290"
     CDS             1794..2759
                     /locus_tag="IVP39_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587347.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2746..4068
                     /locus_tag="IVP39_002291"
     CDS             2746..4068
                     /locus_tag="IVP39_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7587348.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4131..4235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4309..5052
                     /locus_tag="IVP39_002292"
     CDS             4309..5052
                     /locus_tag="IVP39_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7587349.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5067..6059
                     /locus_tag="IVP39_002293"
     CDS             5067..6059
                     /locus_tag="IVP39_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7587350.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6071..6808
                     /locus_tag="IVP39_002294"
     CDS             6071..6808
                     /locus_tag="IVP39_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587351.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLPDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6798..7520
                     /locus_tag="IVP39_002295"
     CDS             6798..7520
                     /locus_tag="IVP39_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7587352.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2481 a         1301 c         1647 g         2165 t
ORIGIN      
        1 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
       61 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
      121 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
      181 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
      241 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
      301 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
      361 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
      421 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
      481 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
      541 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
      601 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
      661 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
      721 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
      781 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
      841 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
      901 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
      961 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     1021 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     1081 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     1141 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     1201 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     1261 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     1321 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     1381 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     1441 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     1501 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     1561 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     1621 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     1681 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     1741 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     1801 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     1861 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     1921 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     1981 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     2041 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     2101 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     2161 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     2221 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     2281 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     2341 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     2401 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     2461 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     2521 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     2581 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     2641 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     2701 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     2761 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     2821 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     2881 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     2941 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     3001 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     3061 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     3121 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     3181 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     3241 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     3301 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     3361 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     3421 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     3481 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     3541 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     3601 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     3661 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     3721 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     3781 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     3841 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     3901 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     3961 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     4021 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     4081 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     4141 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     4201 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     4261 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     4321 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     4381 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     4441 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     4501 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     4561 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     4621 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     4681 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     4741 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     4801 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     4861 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     4921 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     4981 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     5041 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     5101 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     5161 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     5221 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     5281 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     5341 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     5401 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     5461 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     5521 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     5581 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     5641 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     5701 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     5761 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     5821 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     5881 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     5941 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     6001 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     6061 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     6121 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     6181 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     6241 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     6301 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     6361 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     6421 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     6481 cttgttacga acaatcatga atccttcttc ttacttgctt cctgatgaac catttggagc
     6541 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     6601 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     6661 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     6721 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     6781 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     6841 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     6901 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     6961 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     7021 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     7081 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     7141 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     7201 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     7261 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     7321 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     7381 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     7441 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     7501 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     7561 ctttctttgt ataaaagtaa actgtttgat taat
//
LOCUS       DACCSK010000116         7567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCSK010000116 DACCSK010000000
VERSION     DACCSK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.119"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(89..1090)
                     /gene="ccpA"
                     /locus_tag="IVP39_002296"
     CDS             complement(89..1090)
                     /gene="ccpA"
                     /locus_tag="IVP39_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7587353.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            1300..2403
                     /locus_tag="IVP39_002297"
     CDS             1300..2403
                     /locus_tag="IVP39_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7587354.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(2465..3067)
                     /locus_tag="IVP39_002298"
     CDS             complement(2465..3067)
                     /locus_tag="IVP39_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7587355.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(3070..3510)
                     /locus_tag="IVP39_002299"
     CDS             complement(3070..3510)
                     /locus_tag="IVP39_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7587356.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(3636..4574)
                     /gene="galU"
                     /locus_tag="IVP39_002300"
     CDS             complement(3636..4574)
                     /gene="galU"
                     /locus_tag="IVP39_002300"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7587357.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(4589..5614)
                     /locus_tag="IVP39_002301"
     CDS             complement(4589..5614)
                     /locus_tag="IVP39_002301"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7587358.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(5658..6488)
                     /locus_tag="IVP39_002302"
     CDS             complement(5658..6488)
                     /locus_tag="IVP39_002302"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7587359.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(6503..7441)
                     /locus_tag="IVP39_002303"
     CDS             complement(6503..7441)
                     /locus_tag="IVP39_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7587360.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         2034 a         1659 c         1363 g         2511 t
ORIGIN      
        1 aaaattttgt cataacccct tttctcgaat aaacgttgtt ccagaagttg gccacttttg
       61 gaacaacctc taaatgattg atcttttatt atttagttga tcctttttct tcgattccat
      121 aaggcaagat aatcgttttt tcttcgactt cttctttatt catcattttc gtcaataggc
      181 gcatggatac cgctcctaaa tcgtataatg gttgtgtgat acttgtcaaa cgaggacgag
      241 ctacttctgt cagtaaagaa ttgttgcttg tgatgatttc gaattcttct ggtactttta
      301 cgccacggtc taataaacca tccaagatac caatggctaa ttcatcgtct gaaacataga
      361 cagctgttgc accgctatta tggatccggt caactagaga cagaccatct ttaaagttgt
      421 attgtgcctc aaagattaat ccttcgctat atggtagatt gttttcgctt aatgcttctt
      481 tatagccttt tagacggttt tgaccattga taggatcaat cagcgcaccg ctgacaaaag
      541 caattttttt attgccgctt tttgctaatt tatttactgc atcttttgtt gctgatacat
      601 aatcaatgtt tacgctacct acttgttcat ctggatcaat ggaaccagct agtacaacag
      661 gtgtttttga acgtgaaaat tctccacgga tttcgtctgt gatatggtga cccataaaga
      721 tcacgccgtc cacttgctta gccaataaat tattcaatac attgacttct ttttgatcgt
      781 tgccatctga gtttgctaag atgatattgt atttgtacat tgttgctaca tcgtcgatac
      841 cacgtgctaa agaggcaaag aacatattgc tgacatcagg aatgattacc ccaacagttg
      901 ttgttttctt acttgccaag ccacgtgcga ctgcatttgg acgataatcc aagcgatcga
      961 taacttccaa gacttttttt cttgttgctg gtttgacgtt tggattgcca tttacgacac
     1021 gtgaaacagt agccatcgaa acatttgcct ctctcgctac atcatagata gtgattgttt
     1081 gcttttccat ttgtgttctc ctattcatat tttatatgtt ttctgaaaat acgtcttaca
     1141 tttctaaaat aggatatcaa ataatctcaa caattgcaac cgttttatct ctgttttcat
     1201 gaaaattttt acttttttca gttttaagaa cgttggtctt ttaatcaatc agtggtacaa
     1261 ttattgaata caaaattaat ttagaaagta ggttgccaaa tgaataacgt aaaaatcaat
     1321 gaactacgtc aatggatggc acaacaccaa gtagaattgg cttatatcag cgaccctggc
     1381 cacatcgctt acttttccgg atatgaaagt gatcctcacg agcgtgtact agctcttttt
     1441 atccctcttg aacaagatcc tttccttttc actcctgctc tggaagttga agatgctcaa
     1501 aacagcagct gggcgtacga tgtacgtggc tacttagaca gcgaaaaccc ttgggagatc
     1561 atcggaaaag agcttcgcgc acgctatggg caacctggta atgtagggat tgaaaagaat
     1621 gctctgactg tcgatcgctt cgatgctatc tctgatatct tgacaggtgc agattcttac
     1681 actgatctaa cttctattat ccagcgtctg caactgatca agacggtaga agaaaaagac
     1741 aaactgatcg aagctggtaa atgggccgat gttgcctttg aaattggctt caaaacagta
     1801 aaagaaggcg tgactgaaca agcaattatc gcagaaatcg aataccaatt aaaaaaacaa
     1861 ggtgtttcac aaatgtcttt cgacacctta gtccttgcag gagcaaatgc tgcaagccct
     1921 catggtacac caggaagcac gaaggtttca ccaaatgaac tagtgttatt cgatcttggc
     1981 gttgtttgga atggctattg cagtgatgct actcgtacag ttgcttatca aaaaccaaca
     2041 gaattccaag aaaaaatcta caacatcact cttgaagcac agcttgccgc acaagaggca
     2101 gttcgcccag gtgtgacagc aggtgaactt gatcaaatcg cacgaaacgt aatcaacagc
     2161 tatggctacg gcgaatattt caatcaccgt ttaggtcacg gaattgggac aaccgtccac
     2221 gaattccctt ctcttgtaga aggaaacgac ttagtgatcg aagaagggat gtgcttctct
     2281 cttgaaccag gaatctatat tcctgaaaaa gtaggggtac gtatcgaaga ttgtgtgtat
     2341 gtgacaagtg atggttgtgt gccattcacg actacaccaa aagaattact tgttttagag
     2401 taatgaaaat aaaaaaggtg ggagctggtc cgctctcacc tttttattct agaaatgagt
     2461 attcttagtg attggtagtc gaatctgtca ttgtatcttt catttctttt ttagcttctt
     2521 ttgcatcttc ttttgccatt ttcaacattt cttttgtgtc atctgcaact ggttttgctt
     2581 ctgacattcc ttcattgacc gtctctcctg ctttatctgc agcatctttt acatcgatca
     2641 tgatatcttc tgaagtatct ttagcaatac tacctaattc gtctgctggt tttttcagat
     2701 cttctgctgt ttttttgaaa cgattagata aatcagctga ttgttctttg aggtttaaca
     2761 agatatcatc tgattcactt cctgcttcct ctgctgattc acttaccttt tgcttaacat
     2821 tacctgctag atcccctgct tgttgagaaa gatcttgcgc tttttctttt gcaatgcctg
     2881 ttaactcagc tgatttttct tttgccatat ctgtgtaatc tgaagccatg tccatcaaat
     2941 catctgcttg agcagccaat tcatcccgaa gttctttacc agatttgggt gctaacaata
     3001 atgcggcgat tgctgcggct gtacctccaa ctactgcacc taataaaaat ccaccttttt
     3061 tagccattct tattttcctc ctgtcgtttc agtatgtttt ttgtttttaa aaaatttcat
     3121 cgctgttcca ccgacttttc cggcaacagt cgctttagct gtcgtttttc cgattccgcc
     3181 gactttcgtc acgatgtttt tactagaatg attaagttcc gatacacttt cacttaaatc
     3241 agcaactgct gtgaacaatg gatcgatcgt tgcgactttt ccattgatat ccaagagcag
     3301 ctcattactt ttgaccagca agccttctac ttgtctagat aaaagatcta cgtcttttgt
     3361 gacgatctca attgtcgtat ttgcttctgc gacagtctgg tctacttttt ctaaagtttt
     3421 ggaaacttgc aacaatacgc gaatgatgaa aacgacaagt atcgcaaagg cgattgctgc
     3481 gatcaatcct gcaatttctc ctcctgacat gatcaaactc ccttcttgtc tctcttttct
     3541 ataaggatag cattttcgtg ttttttaaac aaataaaaac gagcttcaac tactttttat
     3601 tcaattagca gctgaagctc gttagaaaaa aatgattatt ttttcttttc ttctttgatt
     3661 tctgttattc cttttggatt atgatcgatc ttgcttcctt ctgcttctaa tgttttacct
     3721 aaagacatga tgtattcacg caaaggtgcc cctacttctg gatgtgtcag accatattca
     3781 atacttgttt tcataaaacc aaatttgtca ccaacgtcat aacgtttacc tgtgaattta
     3841 cgggcaaata cgcgttgtgt tttattcaac gtatcgattg cgtcagttag ttggatctca
     3901 tttccagcac ctggttgttg ttctgataat acatcaaaga tttctggagt caataagtaa
     3961 cgcccaataa tagctaaatc acttggtgct ttgcttggat ctggtttttc aacaaagttt
     4021 ttgacattgt ataacccatc ttccagtact tcccctgggt tgataattcc gtattttgaa
     4081 gtttcatcgt gaggaacttg cataacggca atcgtagaag cgtgtgtacg ttcatagtca
     4141 tcgatcaatt gctgtgtcaa tggtacttcg tcttccatga tatcatcgcc tagcataacg
     4201 acaaacggtt catttccaat aaaggctctt gcttgaagaa cggcgtgtcc taaccccttt
     4261 ggatgagatt gacggatgaa atgcaagttg acatctgtcg tttcttctac taatttcaat
     4321 aattcgattt ttcctttttc acttaggttt gtttctaatt cgatatttga atcaaagtga
     4381 tcttcgattg gtcgtttagc ttttcctgta acgatcaaga tgtcttcgat tcctgatttc
     4441 aacgcttctt caacgatgaa ttggatcgtt ggtttgtcca cgattggcaa catttccttt
     4501 gccatcgcct ttgttgctgg taaaaatctt gttcctaatc ctgctgctgg aataactgct
     4561 ttttttactt tcatatacat tcgctccttc aaaaaattgt taatgagaat tcattttctt
     4621 ttttcccatc acgtaacatg atttcttttg cggctgtttt gatttctttt tgttcataca
     4681 gcacttcata gattgtttga gtgatgggca tttcgatttc catcatttgg gctagttcca
     4741 tcgctgcttg agtagtattc acaccttcaa cgaccattcc catattggct agcacctctt
     4801 ctagaggctg tccttgtcct aacaagttgc ctgcgcgcca gtttctggaa tgaacacttg
     4861 tgcaagtaac gactagatcg cctactccgc ttaagccaat aaaggtcagc gggttggcac
     4921 ccattgctac acctaaacgg ctgatctcag ctaatccgcg cgtcatgatt gccgcttttg
     4981 cattatcgcc aaaccctaag ccgtgaatag caccagctcc aatggcaata atatttttca
     5041 aagctgcccc catctctaca ccaaccacat cagggttagt atagattcga aaataatggt
     5101 tcatgaacag ctcttgtaca tattttgctg cttttgcatc tgtgcttgcc gctgtgatcg
     5161 tcgtaatgtc atgctttgct acttcttccg catggcttgg cccagaaaga acagcaacag
     5221 cttttctttt ttctgtcggg atttcctcta acaagatttc agagattctt ttatgcgttc
     5281 cctgttctaa tcccttactt gcgtggatga tcactggctt cgtattcaat ttggcaacta
     5341 gttcttttgc aacagaacgt atcgctttcg tcgggataac gaatagtacc gcatctgcat
     5401 cttttactgc atcttccata tcggtgaatg ctcggatgga atcgggtaga tgatgatctg
     5461 gtaaataacg ttgattggtg tgttgggtgt tgatttcttc tacttgtgaa gcacgatgtc
     5521 cccaaatccg aacgtcatgt ccgttttcag cgaggacttg tgctaaagca gtcccccaag
     5581 agcctggtcc taaaaccgct actctataat tcattttacc actcctgaaa tttttttcat
     5641 ttttaacgct ctaaaaatca aataatttgt tgattttttc ctaatgagcg gtcataatcc
     5701 ggtaaatttc ctttttttct acgccaaata aacagaatca atgtgccgac aagtaaaaca
     5761 gcagacaata attgagaaat gcggatcaca cctaaaaagt aaaggctgtc tgtgcgtaat
     5821 ccttcgataa agaaacgtcc gaaactatac cacattagat aacataaaag gacttctcct
     5881 tttttcaaaa gatgcggttt cttccgcaaa accaaaagca gaataaatcc aatcaaactc
     5941 caaagtgatt cataaagaaa ggttggttgt cgatagatcc cttcgatata catgttatca
     6001 atgatgaatt gcggtaaatg cagactttct aagaaacttc gtgtcgtgtc tggaccatac
     6061 gcttcgtggt tcatgaaatt tccccatcgt ccgattgcct gcgccagtaa tacacttggt
     6121 gcagcgatat ctaaaaaggt ccacaaagaa ataaaatgat atttggtaaa aaagtacagt
     6181 gtgattgcac ctgcaatcaa tcccccgtaa atcgctaatc ctcctgaacg gatattgaat
     6241 atttggattg gatcagctat atattgggga agatcgaata aaacataata caaacgggca
     6301 ccaataatgg aaatcggcaa tccccacaac ataaaatctg tcacgtcatc tgcttttaat
     6361 cctacccgaa cagcttctct gctgctgagc cacatggcaa taatgattcc tgatacgata
     6421 atcaatgcat accagtagat aggaattcct acaagctcaa aagcaactcg attgatttgt
     6481 gctaacatat tgtcactcct tgctagtcat tgcctgagtt ttcttcaatc aagcgactta
     6541 atctttcttc aaatgttttc gttgcgtcaa agcccattgt acgcgcgcgg aaattcatcg
     6601 cggctacttc aatgatgatt gcaacgtttc gtcctgtttt gacagggatt tttatttgtg
     6661 gaatgcccac ttccgcgatt tcaacggtcg tgtcttcaga acccaaacgg tcgtatttct
     6721 tatctttcgc ccatgcttct agataaacag ctaactggac ttgcatagag cctcgtaccg
     6781 cactcgcacc gaaaagattc attacatcta tgatcccgat ccctcgaatc tcaatcagat
     6841 gttccaggat tttaggaggt tctccgatta ccgtcagttc atcttgctga tagacatcta
     6901 ctcgatcgtc tgcgatcaat cgatgtccgc gcttaatcag ttccaacgcc gtttcacttt
     6961 tccctatccc actgtcccct tgaattagta cacctaatcc gtagacatcg actaacacac
     7021 cgtggacact tgttcgaggg gctaagcgcc catctaaata actagataat tcacctagca
     7081 gccgagacgt tgagataggc gaacgcagca cagctatccc attttcattg gctgcttgga
     7141 tcatttcttc cggagccggc aatcctcttg aaataataaa tgcgggcata tcttcactac
     7201 ataatcttct catgatgatc aaacgctctt ctgggatcat ccgttccgca aaactgattt
     7261 ctttactgcc aaatagctgt aaacgattat gagaataata attaaaataa ccggtcaact
     7321 ctagtcctgg gcgtgagatg tccccagtca cgatgacgcg gtctaaactt ttctcgtcgc
     7381 ctgtgacgat ttccaaagat aattttttta ctaattggct aacttttaca acttctgaca
     7441 ttcatgctac ctcatttcta ttagagtgtt ctctaccatt ttatcattgt ctagccaaaa
     7501 gtactaccaa ttaaaaaaaa cgtttttatt taattaccaa tgtaatagaa aacgcattct
     7561 taattat
//
LOCUS       DACCSK010000117         7554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCSK010000117 DACCSK010000000
VERSION     DACCSK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.120"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(102..626)
                     /locus_tag="IVP39_002304"
     CDS             complement(102..626)
                     /locus_tag="IVP39_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7587361.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IVP39_002305"
     CDS             complement(619..1092)
                     /gene="tsaE"
                     /locus_tag="IVP39_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7587362.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IVP39_002306"
     CDS             complement(1215..2195)
                     /gene="pta"
                     /locus_tag="IVP39_002306"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7587363.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2348..3028)
                     /locus_tag="IVP39_002307"
     CDS             complement(2348..3028)
                     /locus_tag="IVP39_002307"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7587364.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3154..4023
                     /locus_tag="IVP39_002308"
     CDS             3154..4023
                     /locus_tag="IVP39_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7587365.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4049..4564
                     /locus_tag="IVP39_002309"
     CDS             4049..4564
                     /locus_tag="IVP39_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587366.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4611..5174)
                     /locus_tag="IVP39_002310"
     CDS             complement(4611..5174)
                     /locus_tag="IVP39_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7587367.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5229..5384)
                     /locus_tag="IVP39_002311"
     CDS             complement(5229..5384)
                     /locus_tag="IVP39_002311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587368.1"
                     /translation="MRKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIVTHGYFITE
                     KIEDFLK"
     gene            complement(5484..5993)
                     /locus_tag="IVP39_002312"
     CDS             complement(5484..5993)
                     /locus_tag="IVP39_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587369.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6089..6469)
                     /locus_tag="IVP39_002313"
     CDS             complement(6089..6469)
                     /locus_tag="IVP39_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7587370.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6799..7548
                     /locus_tag="IVP39_002314"
     CDS             6799..7548
                     /locus_tag="IVP39_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7587371.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2144 a         1567 c         1339 g         2504 t
ORIGIN      
        1 tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct
       61 tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc
      121 attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct
      181 tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg
      241 tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc
      301 ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc
      361 acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct
      421 tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct
      481 tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc
      541 ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc
      601 aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt
      661 gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt
      721 atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt
      781 tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc
      841 tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata
      901 ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc
      961 aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc
     1021 acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga
     1081 tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa
     1141 tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag
     1201 ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat
     1261 acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga
     1321 attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct
     1381 gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct
     1441 ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg
     1501 tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc
     1561 gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac
     1621 aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc
     1681 acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc
     1741 atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga
     1801 acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac
     1861 gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat
     1921 gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta
     1981 tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt
     2041 atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca
     2101 gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga
     2161 acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata
     2221 tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa
     2281 aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc
     2341 tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga
     2401 gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg
     2461 gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt
     2521 cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt
     2581 caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt
     2641 cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat
     2701 ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag atatttctta
     2761 acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt
     2821 ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt
     2881 ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt
     2941 ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt
     3001 gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca
     3061 cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact
     3121 aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg
     3181 gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt
     3241 tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct
     3301 cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag
     3361 gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc
     3421 tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt
     3481 ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat
     3541 ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg
     3601 cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta
     3661 aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt
     3721 tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat
     3781 aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta
     3841 gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga
     3901 gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg
     3961 gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg
     4021 tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct
     4081 tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt
     4141 tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt
     4201 tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt
     4261 cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg
     4321 tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt
     4381 caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt
     4441 ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta
     4501 ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag
     4561 ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc
     4621 ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca
     4681 tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg
     4741 gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct
     4801 tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt
     4861 tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc
     4921 agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact
     4981 ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg
     5041 attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt
     5101 tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc
     5161 gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg
     5221 caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg
     5281 attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt
     5341 tcgactacat tattgacttc ttcgtatgca aattttttcc gcaattaggg catttcattt
     5401 tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa
     5461 aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca
     5521 tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc
     5581 acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg
     5641 aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt
     5701 acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg
     5761 tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca
     5821 gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc
     5881 cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata
     5941 ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat
     6001 tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa
     6061 cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg
     6121 gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt
     6181 cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa
     6241 gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa
     6301 aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc
     6361 ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct
     6421 gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc
     6481 tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat
     6541 tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac agaaagtagg
     6601 tcctccaaga cactcttcct gttcccaata taataaatga aagtaagaaa ttatattttc
     6661 aagaaaacaa acagaaaatt atcataacac aaattattta taattgaaag actagttttt
     6721 tttcaagaaa agcatacaat agattgtgtt agtaaataca gaacacacta catttagtgt
     6781 cttaaggagt cctcatacat gaagtcatat tttcagtttt tagctcatca gctaaaaggc
     6841 tggccgcaac aaaattatta tcttttcttt ttcagtcttg gctgccaggt catgactctt
     6901 gtcaatagtc ccatcacttg gctgtccgtt attacattta tcggtaccac gcttggtgta
     6961 ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg tattttgtct
     7021 gcgatttgtt ttattatcgt tgggttttca gcaaagaact atttaagtat cggtgaacag
     7081 attgcttata tgatcacatt ggatatcccc gtattgttaa gtaaagaatg gaatcataat
     7141 atggcttcaa aaatccgaaa attcactgga aaaagctggt ttatcgcaat catcagtact
     7201 ttccttgtct atcttgtttc aggattcctg attggtcgtt atacagatga tcctcggccg
     7261 tgggtagatg ccattgcttt ttctattagc ttgactgctg gtatcatcag tttcttgcgt
     7321 tacaacaacc aatattactg gtggttagct tcagggcttg ctcaaatggt cctttggtat
     7381 atttcattta cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc tgtttacttg
     7441 ctgaatgatg tccttgcatt taccgtttca ccttggtata accaaaaaga acgtgaacgt
     7501 atgcaaaaaa tggaagaaga atactacaac gcacaacaac acgcataaaa aaac
//
LOCUS       DACCSK010000118         7550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCSK010000118 DACCSK010000000
VERSION     DACCSK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.121"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(42..2207)
                     /locus_tag="IVP39_002315"
     CDS             complement(42..2207)
                     /locus_tag="IVP39_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7587372.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2467..4761
                     /locus_tag="IVP39_002316"
     CDS             2467..4761
                     /locus_tag="IVP39_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7587373.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4754..5419
                     /gene="pgmB"
                     /locus_tag="IVP39_002317"
     CDS             4754..5419
                     /gene="pgmB"
                     /locus_tag="IVP39_002317"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7587374.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5412..6419
                     /locus_tag="IVP39_002318"
     CDS             5412..6419
                     /locus_tag="IVP39_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7587375.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6441..7460
                     /locus_tag="IVP39_002319"
     CDS             6441..7460
                     /locus_tag="IVP39_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7587376.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2349 a         1650 c         1416 g         2135 t
ORIGIN      
        1 tcttatgact tgatgcagga cagctttttc acaacctctc cttacatttt aatggagccg
       61 atcactttac cagctgtcac tgtacctaat tgcttgatca ctacttcttc tactttatcg
      121 tttgtaaata cgatcatgat cgttggatct ttttcttcgt tttggatcac atcaagatcc
      181 atctcagcta atttcgttcc tgttcctact tcttggcctt ctttgacagc aatctcaaaa
      241 gctggctgat tcatttggac tgtatcgatt cccatatgga tcaaaacttc gataccatct
      301 ttcgtttcca gcccaattgc atgtttgcta gggaaaatac ttgtcacttt tccagcgatc
      361 ggcgcaacaa cttctcttga tgttggaata acagcaaagc cgtcacccat cattttttgt
      421 gagaataccg gatcttttac ttcagataaa ggaatcactt ctccagaagc tgcggaaact
      481 agttcttgtt ctatacctaa gtattgctta ctacctgctt gtccatttcc tgtagaaacc
      541 atttctggct ctggaatctc tgctcctgat tgcagcagat cttcgatatc tgatttcaag
      601 acatctgctt ttggtccata aactgcttgg actccattgt ctttgacgat caatcccatc
      661 gcccctgctc gtttccaggc ttcttcagtc cctacttttt ctcgatcatt tacacttacg
      721 cgtaaccgag tcatacaagc atcgacatct ttgatatttt cttttcctcc cagcaaatga
      781 atgatctgga ccacttgctg atctgctgtt cctgttccgc tgttagatac aacggtttct
      841 tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag caaagttgaa ttttttgatc
      901 aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga tcacacacaa cacgaagttg
      961 ataagatctc cacctagacc cgctttgata gctaacggtg tccgagtcag caattcgata
     1021 ttcccaaatg aatggacccg caatggcaag atatctgcca tcgcaaatgc agccccttgg
     1081 atcactgaat aaatgacata taatggcaca gcagcaaaca taaacatgaa ctccaatggt
     1141 tctgttacac ctgttagaaa gacagccaac gctgctgaaa aatacattga tttatatctt
     1201 gctttcttat caggatcgac attacgatac atcgcaaaag ccattcccat caagatacct
     1261 gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg tccagttatg caacacaaac
     1321 tcatattgcg acatatcccc tgcaccttta agattcacta aatcagtggc ccaagccaac
     1381 cataacggat cttgcccaaa tacttgtgtc cccgcctgtg cgcctgataa aatctcatac
     1441 gatccgccaa gctgtgtata gttgatagga atcgtcagca tatgatgcaa tccaaatggc
     1501 aatagtaaac gttctaatgt tccaaataaa aacggtgcta gaatcggtgc gctttcttgt
     1561 gattctgcaa tccataaacc aaaattattg attccagcct gtacgtttgg ccacaccaca
     1621 gcaagaatca atgccacgat tgttgaccat aagataacaa caaacggaac aaagcgcttt
     1681 ccattgaaaa atgacaaagc atccggcaat tttctataat tatagtactt attataagcc
     1741 attgccccga caaatccagc gatgatccct acgaatactc ccatattcaa agctggtgct
     1801 tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg taccgaacag agtgtgggta
     1861 aatgcatctt cattcgcaag catgtcactt gtgacaccaa agatagcccc agtgatccga
     1921 ttgattaaaa taaatgagat cccagctgca aatgctccgc ctgctctttc ttttgcccaa
     1981 cttcctccta ttgctaaagc aaataaaata tgaagattac caataattgc ccaaccaata
     2041 ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat caggattgat caagggaatt
     2101 gatttcccta tactgatcat caacccagct gccggcataa ctgctaccac aaccattaaa
     2161 gcctttccaa atttttgcca aaactcaaaa ctaaatatct ttttcattcc gtttagctcc
     2221 tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt tttctttttt gtaaatgctt
     2281 tattttttat ttttctgtct ataccattct ataaaaccta taatacgtgt gttcccttct
     2341 ataattttct cctaaaaatt tattttttta agcttttttc tatgaaatat tttcttttgt
     2401 ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct cattaggagg
     2461 agaaaaatga aacagatcaa acgactattt gctattgatc catggaaaat cagcacgaca
     2521 caattagata aaaaaaatct tcgactgcaa gaatctttaa caagtattgg aaacggttat
     2581 atggggatgc gcgggaactt tgaagaaact tactcagggg accaccatca aggtacttat
     2641 ttggcgggtg tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa tggttatcca
     2701 gaatactttg gaaaagtgat caacgccttg aactttatcg cgatagatat ttcgatcaac
     2761 gatcagctga tcgacttggc ttctttggaa ccagaagact tttactggga attagatatg
     2821 aaaaatggtg tattatctcg tacattcaca atcactactt ctactaataa agtacgcatt
     2881 tccttcgaac gttttctgag cattgtcaaa aaagaatcag cttatatcta tttaactata
     2941 gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa
     3001 aacgaagaca gcaactatga tgaacatttt tggaatgaga ttgatcgtgg aactatagaa
     3061 gaaatcggct atttgacgac taaaactatt ccaaacaact ttgggattga acaatttact
     3121 gttactgcag caatgcgcca tttcgttaat ggaaaatcag tcatccctga ctatcacgaa
     3181 gatactttag cattacatgg ttgcttgtct gcagaattat ctcaaggtga gacatggtca
     3241 gtggaaaaag aaattatcgt tcttactagt agagatattc cagaagcgca gcaagcagaa
     3301 aaagctgtct cagaattaac gaaccagaca aacaattata tccaagcaaa agaagagcaa
     3361 acagcggctt gggcagaacg atggaagctt gccgatgtgg tcatcgaagg agacgaagaa
     3421 gcacaacaag gtattcgatt caatttgttc caactattct ctacttacta cggcgaagat
     3481 gaacgattga atatcggccc caaaggattc acaggagaaa aatacggcgg agccacttac
     3541 tgggatacag aagcgtatgc tgtccctctt tatcttgctt tagccaaacc tgaagtaact
     3601 aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag caatccacaa cgctcagcaa
     3661 caaggattaa aaggcgcgct ttatcctatg gtcaccttca ctggtgtcga atgccataac
     3721 gagtgggaaa tcacattcga ggagattcac cgaaatggcg cgattgccta tgcaatctac
     3781 aattatacga attacaccgg cgatacggaa tatttgaaaa atgaaggact ggaagtttta
     3841 gcagaaatcg cacgcttctg ggcagaccgt gtccattttt caaaacgagc aaataaatac
     3901 atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa caattggtat
     3961 acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt aacttatcaa
     4021 aaagaagctg ctatcactat cagcgaagaa gaactagcca aatggaaaga tatcgcagag
     4081 aacatgtact tcccagttga cgaagaatta ggaatctttg tccagcatga cacattctta
     4141 gataaagatc tcatgcctgt atccgatatt cccacaagcg atcttcctct gaatcagcat
     4201 tggtcttggg acaaaatcct tagatcttgc ttcatcaagc aggcagatgt actacagggg
     4261 atttatttct tcaatgatac ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat
     4321 gaaccaatga ctgtccatga gtcttccctt tcgccaagta tccattcgat tttagcagca
     4381 gaattaggaa tggaaagcaa agctgtagaa atgtaccagc gaacagctcg cttggatttg
     4441 gataattaca ataacgacac agaagacggc ttgcacatca cttccatgac tggtagctgg
     4501 ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc atgatcagtt gagattcgca
     4561 ccatttctgc caaaaacttg gaaagcttat tctttccata tcaattatcg cggacgttta
     4621 ttgttcatct cagtcacaga tgaacaagtt actttcgagt tattagaagg ttctcctcta
     4681 gaaatttacg tctatgaaca gtcttattct ttagcggatc gtctagaaat aacgctaaga
     4741 aaggaggaag caaatgttta aaggggtatt gtttgattta gacggtgtca ttaccgatac
     4801 tgctgaattc cattatcgtg cttggcgaaa gcttggtcag gaaatcggca ttacgatcga
     4861 ccacacattc aacgaacagc taaaaggcgt cagccgagaa gactcactct ctttgctttt
     4921 agcttatggc ggaaaagaac actctttttc aaaggaagaa tttgcagaat tagcgaaaag
     4981 aaaaaacgat tattatttag agatgatcca gacgatcgaa ccaaaagatg tcttccccgg
     5041 ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc
     5101 aagtaaaaac gggccattcc tacttgaaaa aatgggtctt acaccttact ttgatgctat
     5161 tgccgacccg gcagaagcaa caaacggaaa acctgcgcca gatatcttcc tccttgcagc
     5221 aaaagctgtc ggactaaaag cagaagaatg tatcgggatc gaagacgcgc aagccggtat
     5281 ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg
     5341 agaggagatc ccgcttgtcc ctgatacgac ttttcttacg atcgattact tgaaagaaaa
     5401 gtggcatgac catggataat ccagagatct ccgtcgtacc agttccagag actgaaataa
     5461 agaaaatcac attgaagaac gactacttag aagtcgttct tctcaattac ggtgcccgcc
     5521 tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg
     5581 atcgtttcga agatgttcta acagacaaaa gcttttttgg agcaactgtc ggacctgtag
     5641 ccggaaggat ccgggatgct tcttggggtg agcatagatt ggagaaaaac tgcggatcac
     5701 accatatcca cggtggcacg aaaggctggt ctttccaatt ttgggaagta gaagttttca
     5761 aaactccatc atcaataggt gtggtctttt acttaaaaga tgaattttct acctatccag
     5821 gaccaatcac tgctacgatc acttaccgtt taacaaaaaa cgaattagag atgacaacct
     5881 ctgctagtag ccttgttgaa accatctgca atccgaccaa ccatgcctac ttcaaccttt
     5941 caggaaatgg aacaagagac atctatgaac atcagttaac ggtcttttta gatggtgtgc
     6001 ttgaattaga tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa aaactgccaa
     6061 tcgactttag aaaatcccca acactgcaag agatccttgc atgttatcca gatggactag
     6121 atgatgtatt tcttttgcat catcctaggc tttccaaaac ttccttacaa ttattcgaga
     6181 agcattctgg tcgccaaatg actatagcaa cttctaataa aagtatggta ttattttcta
     6241 cgactggatt cgaagctgat ttctctgtca atggaaaaca gatgcattca aactacggat
     6301 tagcaatcga accgcaagaa atccctgata tcgttcattt ccccaaattt ggatcgatca
     6361 acctccatcc tggtcaagaa cggatcagtc agacgattta tcggttctct gcacaataac
     6421 aagagaaagg tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg
     6481 ttgccacctc gaccgtctct cgtgtcatca acgatcatcc tagtatttcc gagacaacaa
     6541 agaagaaagt ccgaaaaatt atggatgaac taggttatgt tcctaacatg actgcccgca
     6601 atctaggaaa acgcatttcc agtgccatcg gcgtaatctt accccctctt gattcaaaag
     6661 agcggatcgg taacccattt tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga
     6721 attatgatat gactacagca atcgctactg ctaaaaattt tgaattgctg ttagagaacg
     6781 tccagcgtat gcatttacaa aaacaagtag atggttttat tctagtgtat tcagatagtc
     6841 aagatactgt cattgattat ttataccaaa accacatccc tttcactttg atcggacagc
     6901 cttatcatca tgagaacgaa atcgtttatg tagataatga taaccagctt ctaggtaaac
     6961 aagcaacaga atttttgatc cagaatggac ataagaatat cctgtttgtg acaaacacga
     7021 ctcatgaaag tttgtatttc gaacgatatt tcggttatca aaaagcaatg atgatggctg
     7081 gtcttcctgc gttatccagt gtagtactag agcatccgga ggattatctg tcctttgaga
     7141 aaacgttgaa agaaaacgaa gccacagcat tggtggtcat tgacgatatc ttcgcccttc
     7201 gcacgatgca gttggcgcaa atgtatggct atgtcattcc agagacactc tccgtcatta
     7261 gcttcaacaa ctcgatcttc tcaacattaa cacaccctta ccttacaagt atcgatatcg
     7321 atatctccga attaggacgt gtcgctatgc aaaagctgcg cgaattgatc catgaagaat
     7381 tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat caaaagagag acagtcgttc
     7441 ctttagaaaa cccatactaa atagctgact cactaaatac gctgaccatt tatcctatgg
     7501 tcagcttttt atgtaaaaga agatactact tctaacacat tgtttatctg
//
LOCUS       DACCSK010000119         7513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCSK010000119 DACCSK010000000
VERSION     DACCSK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.122"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            369..878
                     /locus_tag="IVP39_002320"
     CDS             369..878
                     /locus_tag="IVP39_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP7587377.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            978..1628
                     /locus_tag="IVP39_002321"
     CDS             978..1628
                     /locus_tag="IVP39_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587378.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            2079..3017
                     /locus_tag="IVP39_002322"
     CDS             2079..3017
                     /locus_tag="IVP39_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7587379.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            3030..4082
                     /locus_tag="IVP39_002323"
     CDS             3030..4082
                     /locus_tag="IVP39_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7587380.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            4348..4983
                     /locus_tag="IVP39_002324"
     CDS             4348..4983
                     /locus_tag="IVP39_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7587381.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            5216..5620
                     /locus_tag="IVP39_002325"
     CDS             5216..5620
                     /locus_tag="IVP39_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7587382.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            5753..6796
                     /locus_tag="IVP39_002326"
     CDS             5753..6796
                     /locus_tag="IVP39_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587383.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            6811..7374
                     /locus_tag="IVP39_002327"
     CDS             6811..7374
                     /locus_tag="IVP39_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7587384.1"
                     /translation="MITVVHKERQEKSSSDVQLWVYLGSISTKIQYGYTDSAKKKGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
BASE COUNT         2470 a         1149 c         1561 g         2333 t
ORIGIN      
        1 cggcgaaaaa aataggcata ttaagcgggc taggaggaga aatattcaca tatcattttt
       61 tcattccgca agctgcgtca agttatttgg aatttgtaga acagatgaga gaggtagaag
      121 cagcactaca aactactttt caataagtag gaattataga aattttactc aaaaaatctt
      181 cattaaaaaa tctgactaaa taagttgaga aaacagaata tacttcaata tccagtgatt
      241 tataaatatc attcactcat tacccagcgc cttatcataa gtgatgcggt ttttatcatt
      301 gatcctaaaa atcttagcaa gacaaaaagt acgtaagaaa gtaaagacta aatacttcgg
      361 aggaacaaat gaataacaat gataggttat tgcgattgcg ttatgcgatt gatttgaaag
      421 atacggattt gattaaagct tttgagctag gcggtgtacc actgactaaa gaagaagctc
      481 aggcggtact gaccaaagta caggataaga acaaagataa tgcagaaaat aatgtgtatg
      541 aaaaaaccat caacaaccaa gtttttgacg cattcctaaa tggcctgatt ctcttggcgc
      601 gtggtccgca aaaagataag ccgatagtag acacgccgca aaaaagcaag gaaatcaaat
      661 atatcaacaa tgtgttgcta aaaaaattaa aaatcgcctt gtctatgacc actgatgata
      721 ttttagacgt ttttgctgag gcagaaatct atccatcaaa aggcgaaatc ggggcatttt
      781 tgagaaaaga aggccaacga aattttaagc cttgcggaga taaatatatg cgtaattttc
      841 tcaaggggtt aggaatttat aatcggagaa aagtataaag aaatttcatg agaaatcaaa
      901 acaatggttg ttttgatgag agctagtttt tgtatgagat taaaatatta tcttaatcga
      961 ggagctggat tggttttatg aatgatgaat ataagaatga tgaagataag atgctttttg
     1021 aagagataga gaatcgatgt aggctcaatt ttgagcttcg gggaaagatg tctttaatac
     1081 aacaaaaaaa gtatttagcg aataaatcag agtttactct gggacatgtt gaaaaattaa
     1141 tctcggattg gattagtagt agaagcgaat ttacaaaaat aaaacagccg attaaatttg
     1201 acatgaaaaa acttttgtta aataagagtg agataggtaa tagagatcaa tatattagag
     1261 ctaaagggca agaaataatt gattcattag gtgaaatgag gtcatacaat tatttgtatg
     1321 taacacatag agctgatgga atggtcatta ctgtggggaa atcttcttct aatgatattt
     1381 ttttagatgg agatcttttt tatcaactca atataaatca cttgtctggc actgaaaata
     1441 taattttaag gacggaatat ggcaatgaaa tatttgcaaa atatgatgag attttaaaaa
     1501 attatttaga ctgggcttgg ataattccgg ttgagtcagg agatgctaag aaactggaga
     1561 gattattggg cgatgaacta attaataaaa aagtaccaat tttaaattat tattcccata
     1621 gacaataaaa ggctattttt catgataata ctagttataa ctgtaaaaat ggaagaaaat
     1681 ctgggaaaga agtttttaaa taagtgtatt gttcgatttt aataaataat aagcaatacc
     1741 atccgtctgt gttgcgcaac atcgtactac aatgaatatt ttttcacaaa aagaaaatca
     1801 tccatcgaat gaatggaagg ttttagtata cacgaggtta atgccccctc ttttataatc
     1861 agaggagtga gattttttct atgaatgttt ctattttctt attgctagtc tagtttctct
     1921 tacgatgaaa ttgggattct attccagttt ttattgattt gctgataaaa taacttgaat
     1981 ggtggatata aacatgataa ataagagaag aataacaaaa cgaggttgtg aaaagattct
     2041 caaatgagta taatagaaag aaaaaggagg aagaatcaat gaaaatagca attgcaggcg
     2101 ctggcgcaat gggaagtcgc tttgggttga tgcttcacca aagtgggaac gaagtattat
     2161 taatcgatgg ctgggcagaa catgtccaac aaatcaaaga acatggatta caagcaaatt
     2221 ttaatggtaa agaggtagaa gcaaaactac caatcgttct tcaatccgaa gtagaaaaag
     2281 aagatcaagt tgatctgatt attctattta ccaaagcgat gcagctggaa aaaatgctgc
     2341 aggatatcca atcattaatc aaaaaagata cagaggtctt atgtctatta aatggtatcg
     2401 ggcatgaaga tattattgag aaatttgtac caatggaaaa tatctatatt ggaaatacca
     2461 tgtggacggc aggtcttgaa ggtcctggtc aggtcaaatt atttggaagt ggttcagtag
     2521 aattacaaaa tttaggtgat ggaaaagaag cagctgcaaa aaaattagca gacaaattgt
     2581 ctgaatcagg attaaatgct catttttctg acaatattca ctattctatt tatcgcaaag
     2641 catgtgtgaa cggaacaatg aacggactat gcacaatctt agacgttaat atggctgagc
     2701 taggaaaaac atcgactgct cataaaatgg tggcgacgat tgtcaatgag tttgccaaag
     2761 tagcagcagt agagaagatt gaactagatg tcccagaagt cattgcacat tgtgaatctt
     2821 gttttgaccc ggaaacaatt gggttacatt atccttcaat gtatcaagac ttgattaaga
     2881 accatcgatt aacagaaatc gattatatca atggcgcaat ttctagaaaa gggaagaaat
     2941 atggtgttgc gacgccttat tgtgatttct tgactgagct tgtccatgca aaagaagata
     3001 gtttgaacgt aaaataaagg agagattgaa tggaaacttc acaaaaaata tcaccgaaaa
     3061 tttttctgaa taaagtcctc gctggtacag caacaggaat cattattgga ttgattccta
     3121 atgcagtatt aggtgccatt ttaaaatatt ttagtgcgta tccctttgcg gtaacgatcg
     3181 cacagttagc agttattttt caactagcaa cacctttgat cattggtggt ttgattgcct
     3241 tacaatttgg atttgatccg atgagaatga tggttgtagc cggagcttct tttgttggtt
     3301 ctggtgtagt caaattcaca cctgatttgg gtgaaacggg tgtttacgtt ggtgctggta
     3361 caggcgatat tattaatacg atgctgacag catcgattgc tgtcgggctg attttactga
     3421 ttggaaatag ttttgggtca gtgtcgattg tgttaacacc gattgttgta ggtgtaggtg
     3481 caggtttact tggtttttat ttattccctt ttgtaacagc aattacttct gcaattggat
     3541 cattgattaa taacttcact accttacaac cgctgttaat gtctatcttg attggttgtt
     3601 cctttgcctt tttgattatc tctcctattt caacagtagc aatcggaatg gcaatccaac
     3661 tagatggtgt ttctgctggt gcggctgcga tgggcgtagc tgcaaccact tttgttttag
     3721 tagtgaactc ttggaaggtt aataagtcag gtgtgacaat tgcgattgct ttaggcgcta
     3781 tgaagatgat gatgcctaat ctatttagaa aacctgtgat tcttattcct tgtttagtta
     3841 gtgcagtgat tacagctatt cctgtcgctc tattttctat ttcaggtacg cctgcttctg
     3901 ctggtttcgg cgtggtcgga cttgttggtc cgttggcttc tttagatgca gggctaaatg
     3961 gctttcttgt tgttctttgt tggttagtca tccctgtttt tgcggcacta attactcaat
     4021 ttttatgcga gaaagtattt aaactgtatg atcgagaaga agtcttcaaa tttttaggct
     4081 agtagatttt actaagaaat tttcaatcaa tggatgcgta agtgagatgt caacaacatc
     4141 tgacttacgt ttttttgcct ttttgagtag gagattgatg ctgacaactg aactcttcag
     4201 tgccattcga ttttttaaaa tgtagaagaa actcaccttt atgttattga ttgttcaaaa
     4261 ggcaactaga ataaaaatat gtgttaaaat gagaaaaaat ttgattgttg gatgtatcga
     4321 ataatcacta aaaagtaggg gaagaaaatg atttttttgc aaaaatatgg tttttatttt
     4381 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
     4441 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
     4501 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
     4561 ttgtatcacc tatttgttgg aacatcaaaa acacttgcaa tacttgttgt tgcaacagtt
     4621 ggtttatatg ggataggtga ttgtattttt actggcttat tcagtatcaa tacgaatgaa
     4681 agctcttgga atctatcgac ttggatccat aatataggtt ctgggctagg ctatgcaggc
     4741 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaagtggtca aggtacgctt
     4801 agccattttt atcttgtttt aacagttatt agtttactaa ttgcaggtct ttacggatta
     4861 gcacggattc cgtcgattag tcagtttgcc ttctttaagc aattgggctt ctttcaacga
     4921 ttaagttttt tctttaacta tttgccgatg atttgtttaa gtttttttca actacgaaag
     4981 tagaaaaagc agtgtgaagc tgattaagtg gttctatgat atttggtgtt gttactcaag
     5041 gcgagtaagg acaccaaata tcatagaacc cttctttttt tgtatagata aactgcttct
     5101 atcactgatc atcgatgttg tctaactgat gtcgccgaat gcaagcatta cctttgtcag
     5161 attaaaaaat actcgttaca cttaatatag aacgacgaaa gaaaggatga tctcaatgaa
     5221 aaaaatgtac gaaacagcaa tgatcaatcg tggaggccgt gatggcgaag ttgaggcacc
     5281 aaacggaagt atgcacatga agatcgaccg cccaggaatc catagtgaag ggacaaatcc
     5341 ggaacaactt tttgcggctg gatacgcatc ttgcttcaac ggcgcggttc aacacatgct
     5401 ggaagaaaat aatcttgagt ctgattctga agtcaaagca cgggtatctt tatttcaatt
     5461 agaagatggg ggctaccaaa tcggggtcgt tttagaagtt tctttgccag gacttgagaa
     5521 ggaccaagca gagaaaatcg cagaagaagc ccacgcattt tgcccatact ctaaagcaac
     5581 aagaggcaat attgatgtgg aagtgactat agtagaataa gggattctta atgttactat
     5641 aaatgagatt atgaaagatc ctataaagaa acgctagtaa aagaaacatg tagttacggt
     5701 aattaatagc ggtatatgga cgattgatcg aatagaacaa ggaggcaaaa aaatgacaca
     5761 gcgggtagca ataatgattt tagtgttgct ggtcattggc ctccttgttt actatgtcct
     5821 aaagtttaag cattggaagc agcaacggat acatcaggat attgaaaaaa agttaaaacg
     5881 gtatcctatt gttcaagcag cttgggaaaa ggctgaagca aaacaatata acattcctgg
     5941 attaacagaa acaagaatgg tggtgccgga aaccggcgaa aatgaggttt gtcaatggat
     6001 gacgccgcaa gggttggctt tttctcaaga ttttgtgttt atttctgcct actgctatga
     6061 ccatcagcat cattctatta ttcatgtact ggatagagaa acaggacagc cgatcaaact
     6121 gctgatttta cctaaacggc cacatgtagg gggcttagta tatgatacaa aacgggaact
     6181 tttatggttg acaatcacag gatctgctac tggacgagta gccgctttac gtttgatcga
     6241 tatcttagca gatacatctg aagagacggg acagccaatc gcttattggc tgacaactga
     6301 tctctcagag attccacaag catcgtattt gactcaaaac aatgatcaac tggtatctgg
     6361 caacttcaca ctaaaaggtg aaggacagtt gactttctat ctattgccga cgatagctga
     6421 aatgaaaaca gcaattcgcc gcaaagacaa aatccaacca accgtgacaa tgagcagaaa
     6481 aacatctgat aagattcaag gtgtggcttt ttatggccat tatctcttag ccagtcgttc
     6541 ttatggtcct tatacctcgg aactcttagt gtccgagcgg gactctccta gccggatcct
     6601 aaaacgaatc aaatttccac cgtatttgga acaaatcgtt gttgtagaag atcgtctagc
     6661 agtattgttt gaaagtggcg ccgcagcgta tcgcgagaaa gccaatcctg tgttagccaa
     6721 tgttttatta ctagacttag caacgttgct tcatgcaaat aagcgtccca aaaagattgc
     6781 tgcaactaaa aaatagtcgg acaactcaag atgatcactg ttgttcataa agaacgacaa
     6841 gaaaaatcat ctagcgatgt tcaactatgg gtttatttag gaagtatttc tactaagatt
     6901 caatatggct atacagattc ggcgaaaaaa aaaggcaatg ttaagttttt aagaatcaca
     6961 gatatacaag agggacgtgt aaattggtcc agtgtgcctt actgtgatat tagtaattct
     7021 aaactagttg atttaagatt ggaagaaaat gacattttga tagcaaggac aggaggaact
     7081 atgggaaaaa gtttcttggt taaggaaata agtgaggaat ctgtttttgc ttcttacttg
     7141 attcgaatta ggttagttga gaaattatta tcagaatacg tagactgctt cttagactcg
     7201 ccattatatt ggaagttatt agaaaaaatt tcttacggaa ctgggcaacc aaatgtaaat
     7261 ggtacaaatt tatctaaact acttatacca ttaccacctt tagaagaaca acaacgcatg
     7321 actactaaaa tagaaatgat aagaagatct attcgtagaa tcaattttga gtaaatataa
     7381 cggacctagc atccatttta taccagatga tgctaggtct attatttata ttttatggaa
     7441 tttaattgat ttgttctagt gttttaatat tattacgaca gtaaaaaaat agtttgattc
     7501 ttctaacatg aat
//
LOCUS       DACCSK010000120         7418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCSK010000120 DACCSK010000000
VERSION     DACCSK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.123"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..751
                     /locus_tag="IVP39_002328"
     CDS             <1..751
                     /locus_tag="IVP39_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7587385.1"
                     /translation="GWENFKFLFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMS
                     ELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNE
                     PKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQL
                     IPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTA
                     AAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            763..1683
                     /locus_tag="IVP39_002329"
     CDS             763..1683
                     /locus_tag="IVP39_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7587386.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1724..3181
                     /locus_tag="IVP39_002330"
     CDS             1724..3181
                     /locus_tag="IVP39_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7587387.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3526..4140
                     /locus_tag="IVP39_002331"
     CDS             3526..4140
                     /locus_tag="IVP39_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7587388.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4133..5857
                     /locus_tag="IVP39_002332"
     CDS             4133..5857
                     /locus_tag="IVP39_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7587389.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5854..7305
                     /locus_tag="IVP39_002333"
     CDS             5854..7305
                     /locus_tag="IVP39_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7587390.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2531 a         1232 c         1477 g         2178 t
ORIGIN      
        1 gggttgggaa aatttcaaat ttttattttc ttcggatcaa gcatttctca tcactaaaaa
       61 tacaatttta tataatgtag catttatcct tttgaatctg ctgatctcag tcgtatttgc
      121 aatcattatg agcgagttga gaaataaacg actcgttaaa gtttatcaaa cgatgtcctt
      181 actaccttat tttctatctt gggtcattat cagttacttt gtttacgctt ttttaagtcc
      241 tgacaaagga atattcaatc agtggatcac tggacatgga ggagaagctg tcaattggta
      301 taatgaaccg aaatattggc cgtttatctt ggtgttcatt ggtacatgga aagggattgg
      361 ttataacagc attatttact ttgcatctgt tatgggaatc gatccgactt attatgaagc
      421 tgctatggtt gatggagcta gcaaatggca gcagatcaaa catgtgacga taccacaact
      481 tattccacta atgacgattt taacgatttt agccgtagga aacattttcc gagctgattt
      541 tggcctgttt tataacattc ctaggaattc cggtgcatta tatgatgtga ctcaagtttt
      601 ggatacgtat atctacaatg gcctgacatc cacaggagat tttggtatga cggctgctgc
      661 aggactttac caatcagttg tcggctttgt cttgttgatg atcacaaata cgatcgcccg
      721 acgttttgat agcgattctg cattattcta aggaggaaaa aaatgaaaaa aaagaaagtg
      781 gagagagtcc aagtccgctc tttcaataag acggctaatt ttttctctaa tataatgatt
      841 gcactctttg ctatttcctg tattttgcca tttatatttg ttatcgccat ctcttttacc
      901 gatgagtcca tattgtcgac tcacgggtac agtttttggc cagaaaagtt cagtatattt
      961 ggttatacgt acttgttcga ccagatgcag gataaaattt tccaagcgct tttcgtcact
     1021 gtacttgtta ctgttttagg tacactgatc aatagcacgg ctacttcttt atatgcttat
     1081 gcgatttcac ggtcaaactt tccattccgt cgttttttta ccgttttttg tttgattacg
     1141 atgttgttct caccagggat ggtggccaat tatcttgtta tgacaaatct tcttcagtta
     1201 aaagatacga tttgggcatt gatcttgcca atggctgtca gtcccttcaa tattattgtg
     1261 atgcgcacat tctttaaacg ttcggtctct gattcgatta ttgaatcggc acggatcgat
     1321 ggagctagcg aactgcgaat atttgttcag atcgttttac cgttagctgt tccaggtatt
     1381 gcgacaatca gtttatttgc cgcattgggt tactggaatg actggttcaa tgctttacta
     1441 tacatccaag atgataagct agttccacta cagtatcttt tgatgaaaat ccaaagtaat
     1501 attcaatatc tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg attagcctca
     1561 gttccaggag aatctgccag aatggcgatc gtagtcatct cgacgttgcc tattgcgatc
     1621 agctatccat ttttccagaa gcattttgtc aaaggtctaa caatcggcgg agtaaaagaa
     1681 taaagaaaca aaaatataaa tcataaaatt aggaggaaga aaaatgaagt tatggaagaa
     1741 actagccttt acaggtgttt cagcgttgtt gttgggaaca ttagcagcat gcggaggttc
     1801 tggttctaaa gaccaggcag aagctagcaa ttcggacact ttgcagatgt atcaaatcgg
     1861 agataaacca gataatttcg atcaattgat ggaagtagca aacaaacgaa tcgaaaaaga
     1921 gatcggtgta aaagtcaata tcaattacat tggctggggc gattacgaaa agaaaatgaa
     1981 cgtcatcatt tcttccgggg aaaattatga tatcgccttt gcgaacaact atgtttccaa
     2041 tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg cctaagtacg ctgaaaaagc
     2101 ttataatgat ttagatgaag catatattaa aggaaatctg gtcaatggaa aattatatgc
     2161 tttcccagtc aatggaaatg tctttgctca acaagtactt acttttaaca aagctttact
     2221 agataaatac gatctttcaa ttgatggaat ccaatcttac gctgatgcag aaaaagtctt
     2281 gaaggaattc catgaaaaag aaccaaacac ggcagctttt gccattggac aaggcttcaa
     2341 agtccaaggg aactttgatt atcctttagg gaatacgtta ccatttgcta ttgatctgga
     2401 tggagatgat tcgaaaatca tcaaccaata cgataatgaa acgtatatcg gactgttacg
     2461 tacaatgcat agctggtaca aacaaggact cattccaagt gacgctgcaa cgagcaataa
     2521 ggattatcca ctagaaggaa atacttggct aatgcgtgga gaaacacaag gaccgtatga
     2581 ttatggcgat acaattttga caaatgcagc aaaacaagaa cttgtctcaa aagcaattac
     2641 tgttccacta aaatctacag ctcaagcaca aatggcaaat tttgtcgtat caaatacttc
     2701 aaaaaataaa gaaaaatcag tagaacttct agggctgctt aacagtgatc cagaattatt
     2761 gaatgggctt gtttggggga tcgagggaga agcatgggaa aaagtcgatg gttcagatga
     2821 taaaatcaaa ttgctagatg gttatcagcc aaatacacat atgtctgctt ggaatacagg
     2881 aaacaacaag attctatata cacaagagtc tattacggat gacatgattg ccaaacgtga
     2941 tcaatcgatt gctgatgcag aaacttcacc gattttagga ttcagcttca acactgacag
     3001 tgtcaaaaca gaattaagta atatttctaa tgtcatgaat caatatttag atggattgaa
     3061 tacagggact gttgatccag atgaaacatt accgaaattg aaagacgctt tggacaaggc
     3121 tggctatgat aaggtattga aagaaatgca aaaacaatat gatgagttcc gacaagaata
     3181 ataatgaata aagacaaagg ggttgtgaca aaagacgtgt cacaacctct tgttccgaat
     3241 aaacggtgtt caaaagccag tttctcggta ttaagctaaa tatatgtaaa aatgtggaaa
     3301 gccatattca caaaattttt cttactactc gaaactaagc ggctatttca caaactcttg
     3361 gtaaacggtg ttcaatcagc taaccctcgg tattcgctca caaaacgaca aaatatgagg
     3421 aactattttt ccgtttgttt ctctaataat caggtcaagg tgctgattgc ccaacctttt
     3481 tttgtataat gagaaagtat aaacttaatg tttggaggag aagatatgtt agggaaggtt
     3541 ttggaagagt tatttatccg gatctgggtc attataaaac taaccttgta tttctggata
     3601 tatacatttg ccggcgggat catctttgga ttaggtgcgg catggaaaac agtcaatgaa
     3661 ctattttatt tgtacggatt cgagtataaa gaaatcacaa taaaaagagg ctggaatata
     3721 tataaaagaa attttttaag aggcaatctg ctatttagtt tatttttatc tggtactgct
     3781 ttattgagtt acaatttata tttatctgtt caaatcaaag ggctcatttt tttagctatt
     3841 gattttattt tactttttgc cttgctctgc ctatttgcta cttatcaata cagtatgatt
     3901 ttagatagtg aatatacgat ctctgttcct aatctgctaa agctaagttt tatttcggtt
     3961 ttttccagct tttcgtcttt tctaaaaata attattggta gcggtataat tttaggcgtg
     4021 acttggcaat tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac ggtttggaat
     4081 ggtacgatga caacacattg gcgtgaagaa ttagataaac agttggaaag ttatgaataa
     4141 gtttcggatt ctattttcaa aaggaacctt actaaatcgt ctgttgcgga aatacgcggt
     4201 tatcatgatc ggtgtaacga tgactgctac cgttattttt agtatccata cttgggaaca
     4261 aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt caaagcacaa gcagaatgct
     4321 gaatgataaa acaaccttga gtaggatcat caaaaaccag cttgtaggga attccgaaaa
     4381 gatcgaaaat gtgacgacct acttaacaaa acccatagat cagtatctga tgtatgtata
     4441 cgaacagcaa aacagtaccg atgaattagt ttctttccca aatcaaatca aagatctcta
     4501 cgcaaattat gaagaactaa gtgcgatata tatcgtgttg aatcaactac cggagtatta
     4561 tgaatcttct agagaaaaca aaggtgggga aataaaaaag gggacaccaa aattactaga
     4621 cgccttttat atcaagttgc ctatcacaca aggaggagct gagtcaggaa gtatttttat
     4681 tggttttcaa aagaaagaac tagatatgat tttagaaaat ctaacgtcat tcaatggact
     4741 ctctttatac atgatttctg gaacgacaaa tcgactatac acattccatg ataataaaat
     4801 agctaaagaa acgtttgaaa gacaagaaat gatcatcaca gagagtctga agaaaaattc
     4861 acaattgccg attgacgatc tggaaaaaaa caattttgtt caacaccaag aactgtctga
     4921 tgattatcgt atattagctg tactagataa aaaaagtgta aaaatcgaaa cgggaaaaaa
     4981 tcttgccccg ctgatcattg gtatcattat tttagacaat atactgctta tctttcttta
     5041 taaaacattc aaacgatatt cacagcaagt atctatcgtc atgcaggcca tggatcaaac
     5101 agcagcaggg aatttagaga cgcggatcga cataacaaac acggaatatg aactgaagga
     5161 actttcaatc ggtatcaacg aaatgttgga tagcatcaat cagtttgtag aagacatcta
     5221 caaattagaa atcaaacagc aagacgcaca tatgcgggcg cttcaagcac agatcaatcc
     5281 acacttttta tataatacat tagaatatat ccgaatgtat gcaatcagcg aaggaagtga
     5341 agaattagca gatgttgtgt atgcgttttc cgctttgctt cgaaacaata cgaatcaaga
     5401 gaaaacgatc actttaaaag aagaactgga cttttgcgaa aaatatgtct atttgtatca
     5461 aatgcgttat cctaatcgag tagcctatca ttttatgatc gatcctgatt tggaaaagat
     5521 cgaagtacct aaatttgtga tccagccatt agttgaaaat tattttaaac acggtattga
     5581 ttttactcga ttcgataatg cgctaagtgt caaagtcttg caggaaggca aacgggtaag
     5641 gatcatcatc aaagataatg gaaaagggat gactgaaaaa cggctaaaac agattgaaga
     5701 aaaactgtct catcctaaag tggaacttca cggttctatc ggtctgcaaa acgtgaatga
     5761 acgtttacgt gccagcttcg gcagcagtta ttatatgtcg cttgagaata atgagacagg
     5821 tggactgacc gtttctatta cattcaagga gggatgagcg tgtatcaagt gttattggta
     5881 gatgatgaat acatgatttt gaatgggtta aaaaagataa ttgattggca aagtttaggc
     5941 tttcaaatag tagcaacagc agagaatgcc aaagaaggac tagctgtact tgaacagcga
     6001 cagattgatt tagtagtaac ggatgtgaca atgccagaaa taaatggact ggaatttatt
     6061 gaagcggctc aaaaagaacg tcataatttt gaatttatga ttttatcagg ataccaagaa
     6121 ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga attatttgat gaaacccgtg
     6181 aataagttcg aattggttga aagcctcaag aaaatcaaaa cacggttaga tcagcaaaat
     6241 gaacaaaaaa atcaacaaga aatctatcaa gaactgttat tcagtcaatg gttgaatgaa
     6301 gaattggatg aaataagtga agaagaaata ataggacaaa taagtgagaa gcagcatagg
     6361 atagtattga ttcaactggc aagaactcat gatactctta tcaatagatg gttgcaagaa
     6421 cggcaacaaa tcttttacta tcaacgaaat tacggagacc gtgtattgat cacgttgttg
     6481 ttggaggcag atgaagttga tggcttctgt tgttttgttc aagaaaactt tcctgatcag
     6541 gaatggctga tcagtatcgg ggaagagaca gattctttgg acaaaatccc tgaaacattc
     6601 caacaagcga aagacaatct acagttgcat caattttatg gagataaaga cgagcatgtt
     6661 ttttacgcag tacaagcaga aacaaaagat caaaccatcg atttttcaag tttcagacgc
     6721 gtgctgcaaa ataaacaatt ggaagtcgct caaaaaatga tcactgattt ttttgaacag
     6781 tttcagttag cagccatgat gccggaagat atacgatatt ccagtttttt actgtttatg
     6841 gagattcaac gggaattgat tgatctggaa gatgaggaat atttacaagg aatcgaaaaa
     6901 atccatcagg caaaaacggt gcaagaactt cagcagctac ttctgtcatt catcaaaaag
     6961 catcagagac agaaaaagta cagtgacaat gtggaaaaag taatcgtaat cctgcaccag
     7021 cattatcaag aaccacttac actcaaagaa gtgtcagaaa acctgcattt aaatgtcatg
     7081 tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta tttgaaccat
     7141 ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta atcaaaacat caatgagata
     7201 gcaagtgaga ttggctacaa caacaccacg tatttctcta aattattcaa gaaaatcgtc
     7261 ggcagatccc ctaaagagta ccgagaaaac agtggagaat cataaaagaa aaagccaatg
     7321 taatgaaata acttataaaa aattgtctag aaatctaatt ttgggctgtt cttattactt
     7381 gaggcaaggc agctttttca tgacctctcg ataaacgg
//
LOCUS       DACCSK010000121         7402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCSK010000121 DACCSK010000000
VERSION     DACCSK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.124"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..2322)
                     /gene="esp"
                     /locus_tag="IVP39_002334"
     CDS             complement(<1..2322)
                     /gene="esp"
                     /locus_tag="IVP39_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP7587391.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
     gene            complement(2939..3244)
                     /locus_tag="IVP39_002335"
     CDS             complement(2939..3244)
                     /locus_tag="IVP39_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587392.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3389..4594)
                     /locus_tag="IVP39_002336"
     CDS             complement(3389..4594)
                     /locus_tag="IVP39_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7587393.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5288..5674
                     /locus_tag="IVP39_002337"
     CDS             5288..5674
                     /locus_tag="IVP39_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7587394.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5772..5963
                     /locus_tag="IVP39_002338"
     CDS             5772..5963
                     /locus_tag="IVP39_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587395.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6477..6809
                     /locus_tag="IVP39_002339"
     CDS             6477..6809
                     /locus_tag="IVP39_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7587396.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6719..6922
                     /locus_tag="IVP39_002340"
     CDS             6719..6922
                     /locus_tag="IVP39_002340"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7587397.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            7106..7261
                     /locus_tag="IVP39_002341"
     CDS             7106..7261
                     /locus_tag="IVP39_002341"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7587398.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2471 a         1307 c         1061 g         2563 t
ORIGIN      
        1 ggtgaaggtc actttgtggt agcctgcagg aatgttttct cctggatttt cataaatatc
       61 tttacttaca gttactgcta aatcggtcgt gcctacagaa ccatcttggt aaacaatttt
      121 aataggaatt gttccagtaa tcggctccga ttctcctaca cctttagctg gagtgaatgt
      181 aacaatacca gtatttgcat caacacttgc attaccgaag tccttattat agacatctaa
      241 aattgaaaag gcatctttag ctaaaggaac atctggttta tcaaatgtag gttttccact
      301 ttgaactgtc gtttctggag ttgttgttat aggtacgtat gttgcatcat tttccatagc
      361 ttgagaagct tttacagcaa actctacatc cacgtcgaaa gttcgatttc cgatcttaac
      421 agttacaata cctttcgtac tatcagtaac gttagaagta tcaggctctg ttttccatga
      481 atactctgca tcttcaggta agtttttatt attccgaatt gattctttag catctggttt
      541 aaatccaact accacggttt gtttatcaac cgcttttggt gattccttaa taacggttga
      601 accttcttct ggtttatcaa aacctggaga aacgatttgg atttttaatt catctttcgc
      661 gattaattta cttgaatcta cacccgtaaa ttcaaattct acagtatctg aaattggagc
      721 cccatctttt tcatttggag cgatagtttt ttctgctaat acagtacctt ctttatcaac
      781 aagttgggct ttgtgacctg ttcccgctaa ctcgtggatg aatactttcc ccttaactgt
      841 tgtgtcaaca tcatatactt cttctaattc aggtttcccg atatatggat tcgtgtctcc
      901 gctctcttct ttatcgtcag ggtttccatc accatcaata tcttgcaaat aataataacc
      961 tgttccataa gtgttctgga ttaatttttt attcgtatcc gacaaccaag aatcaaagat
     1021 gaaatcttca ccaaatggtt tattttcttc aacttgtgct gttgctttca tatcttctaa
     1081 gatttcatct aatggcttag tcaattgtaa aacatatcga ataacgactg gacgtggact
     1141 tgcattagca aaatcaataa gtttggtaag atcattatca gtaattgaac ctacaccata
     1201 attttcatga tctttgcgcc gttcaattgc taaatcagcc acttttttcc ctgattcaaa
     1261 agaaacatca tatgttactt tgtcagcctg aaccatgtgc atctgtatac ccgctacata
     1321 tggaactaat cgttcatcaa ttttgtaatt agcataccaa ggcgtgccct ttgcggaagc
     1381 tgaatatgag aaattataat ttttcgttaa cttatgatct aagattatag ctccattagc
     1441 acctgtggat cctgaaccat cttttgtttg agtattaggt tcctcatatt ttgcacttac
     1501 tgatccatgt ttaaactggt caggattatt ttcagataca tctaagctat tatagaaatc
     1561 atctgaatct gttaaaaagt agcccgtact atcaatacct ggtacaattt tatcatcgtc
     1621 ttgatgactt accaagtaga ttcgatacat taaacgatcg gtcttgagat tgtctatgcc
     1681 tatctcttta aaaatttcat ctaagctctt ttcaaacgta attaccccat taggagcagt
     1741 ttgtgtatca gttgtttcag ccccagcaaa taatccatca cttgcacgaa tataattgac
     1801 cttccaaata tttgtaggtc tacccagcga atcattaatt cttacaaagg tccgtctaga
     1861 agatccaatt cctttctttg gatcgacctg aattttttct acatatttag ctaatcgttc
     1921 atctaattga agacggattt cccagttttt agttgatgct gcaatattat gttttccgta
     1981 aacttcaaat ccaattgtat tgccttttag tgtgtctcca gttagttctt gaggactaaa
     2041 ttgtaaatcc gaaaagttat aatttttcgt ataaccagga ttctcctgaa aagtattccc
     2101 cgaagctact ttgtcaccta cttgtacgga gcttatcgct gtttcaagtg gggtgcttgg
     2161 agaaatttca tcttgtgctt gaactagttc agttgttccc aagacaagac caacaccaac
     2221 caaaacagat gcaaccccaa cacttaattt tttgatactg tacttcaata ctcgtttctt
     2281 tgttttttct agaaatactc tcttattatt cttgctaacc attattcaaa tcaactccct
     2341 taatagttat tttcttagta agaataaaag ataaatctga tacgtattat tcacagaata
     2401 ggaatcttac taaacttatg ttaactagtt tacttaaaga atttcatgca cattacaaca
     2461 aaaaaagtac atttcttcat gtttttttcg gatatttgtg ctttttaacc attcagcagc
     2521 tgaaaacata taaaaagata tgtatgtatt ctttctgaaa ttaagttcat ttctatgcga
     2581 tgaagcatgt tttcaaaata ttactctttc ttagattaaa cttcagagta ttcaaatagc
     2641 ggaataagga gccttatccc gcattactga atgagactgt gacataagta aaaaggtgac
     2701 ttcaaattcc ttcttcggaa tttgaaatca ccttctttat aattataaag tatcggtttc
     2761 acagcattcc tgaatgattc ctagaatctt aaacgaatga ttggagttat caacattttt
     2821 tcactcgttt ttagcgaaac aaaaaatgac atttgtcaca gcctcattaa attattgatt
     2881 ctttttcttt ataattgaat tgtccatatt taaagtgtct agtactctac atgaataatt
     2941 aattgctttc actcaaatac ttataatcta ttaacttgta gttgtttaac ttttctgaaa
     3001 aagttagacc cacgtaataa tcttcccagc tttctctttc aattaactga tccaattttt
     3061 gataatccat aaattcatca tgtattaaaa agaataaata acttgatatt ttagcatcta
     3121 gcttaatcag attaaataat gtttgcttaa aattgctacc attaatatga ctgatagatt
     3181 ctctagcttc ttcacataaa ctttttactt tattaattac tactttgtca tattcattta
     3241 acacactatt attaccatta cttagacgct cagattctac gaaacaactc aaatcagaaa
     3301 tttcccaaaa aattttttgt taaaatccaa tttcctcaac tcctccaaac tacgtaaatg
     3361 atatagtctc ataataaaat aaaacctctc agctaatgtt gttgaaatta tccactaaac
     3421 ctaattgata ttgcttagga gatacacctg cgtacttttt gaatactctc gaaaagtgtg
     3481 aagaatctga aaatcctaaa cctaaagaaa cttcactgat agtatggttt gatttcaaca
     3541 ttattttagc cgtagaaatt tttcgcttag agatataatt ataaatcgta atatgcattt
     3601 cattcttaaa gctttctctt aatttagttt cacttacaga aaaataatta gctatttcct
     3661 ttacagtaat tcttttatag atatttaagc cgatgaattg aacgacagaa gaaattaagg
     3721 gagaaagatg ctctatctta gtttttccga tttccttagt ataataaaca atcccagaat
     3781 ccctcacttt taagacctca ggtaacgata cagctaattc gttttcttgt atcaaagcat
     3841 ctctactttg atacgcttct attatatcca ttcctaatgt tatagacgtt tgtgctaact
     3901 tttcaaaaac aatgatagaa taattttttt cagaccgaat cgtatctcca ctaacacttg
     3961 gaattattcc attactcaga ttaaaaacca tgttttctaa atttcgaata tctccatttt
     4021 taacatattc cagtatttta ttttcataaa gaaacagata tatttctgga tcgtaatttt
     4081 gtgataacag attatctatt ttgatttgct cgatagtttt acttagattt cctgtatatt
     4141 caattaacgg ttcatggaat agatcttcta tttttccagt aaaaaaataa tgaactaaaa
     4201 tcagaatatc tcgaatgtct cctaaagaat acaaagtcaa attttgcaat gaattataga
     4261 gtcgttctct atcagccatt gcaacattca aattgttcat ttttagttta aaaaaatctt
     4321 tatcaatatt atttgctcta aatggaccaa ataagtaata tctggtttta aacatgtata
     4381 gaagaaacat ctcattaaaa tcaccagtaa agaaatggaa atctttagtt tttttaatat
     4441 tatcagttag taacttttta taatcataaa aaaaataagt agttctatct gagcgatact
     4501 cttttataat actgaattcg gaattaaaaa ccacgatagg taactttaaa caaacatgaa
     4561 gagactttaa taagacttct ttatctgaaa acacttttgt ttctctcttt cctttctaca
     4621 tcattaaata gtattttata cgttatctaa aatactattc ctctgtcaaa ataagctaac
     4681 ttacttcatt ataaaatatc cacttatcgc tttcatgtat attccaccgt aaaaagtaca
     4741 ttttatcacc gagaactcat catttttcat catgatgaat actatttaca tatatctagt
     4801 tcaaagacaa taattattat gatccgttat tatctaagca ctatacagat aataggaatt
     4861 tttcgtttct aaatactcca atttgggaat ccaataaaaa ttttcgttca aatacgtgtc
     4921 tattcgaact cttttctagt tgttaattat gaagggggaa gaaataaaaa atacaatcta
     4981 tacacgaata agaatattat ccaaaaggag gttttagaaa agatttatta tacgtaaaaa
     5041 gctcaagaca ttttcttagc cataaggaca tgtttgatat ttaagaagat atatgaattt
     5101 tcttttctaa gtataaagat aactatccat tatcaactaa aaaaacattt ttgaaaataa
     5161 acatcagcag tcctatcaat gcatgtgagc ttatagaagt tattcgcagc gcagcataat
     5221 tgcattagct tcttttcgtg ttttttgcct cttttccctt taaatcttaa tggaggaggt
     5281 gtgcaatatg tcagataacg acgaacgcgt aaatgcccag ttgattcgat tctttgaaaa
     5341 ggtcattttg aacaccgcta atacttatta taaaaaacaa gaagaaattc aaaaacatga
     5401 acaacttgat caactcccac catatttgtt tactaaagat actataaaat ttaaacaacc
     5461 gattaatgtt ttaaatgtga ctctccttgt tgaagatata aaattagcaa aaatccttcc
     5521 tttgttgaaa gaacctgaac aaatttttct tatcgaaaaa tttatcttta ataaaactga
     5581 taaagaaatt ggaaaggttc tgggtattac aagacagggt gcgacaaatt taaaacatcg
     5641 actttacaaa aaaattcaaa aaatgctggt ttaatatctg cataataact aattttgtat
     5701 gcttctttgc tcgtcctcct ccttcccaaa aaaataaata gattataagt tcatgggaag
     5761 aaggtaaatt tatgaatact ttaaaagaag atttcatttt agcaaagaat ggaaatgaag
     5821 aagcagttgt ggccattctc aaaagattta gcgctttggt ccataagcaa tcctggagaa
     5881 ctggaaaata tgatcaagat tgttatcaag aatgtatgct tgcaatatat ctagctatct
     5941 cgaaattcga aattaaagaa taactcaact aagggagtct gtgacataag taaaaagata
     6001 gtttcaaatt tcgaacaata aatttgaagc catcttcttt ataattataa agtatcgatt
     6061 ttacagcgtt ctataatgat tcatagaatc taaaacgaat gattttaatt attaacgttt
     6121 tttaattcgt ttttagctta aaaataactt ttgtcacagt ctcactccta aaattacttt
     6181 atttacgtac gaagtgggca ggaggtcgaa tccagaactg ggccatggta cttcaacgag
     6241 gacttctcct ctagaattga aaagcatgcg atttaccttc cgtaacgacg gtagatttat
     6301 tcaactatgt gtccaatcaa aatttgtctg atttcaaaaa tagtcaagag aattttggtt
     6361 gttaaaaact cttatctatc aacttccaca gtttagttga caaaccctga tactaagttg
     6421 ggctatgata cacttttctt gactatcagt tacgttaccg gaaggaatga actctattga
     6481 aaaaaacata tgatcgtaaa tttaaactaa agatttcaca ggatatcctt gagaaaaaag
     6541 taactaccaa gaaaattgct gaagaataca atatctctcg tccaacaatt tcccgatggg
     6601 tttcagaata tcgtcgatac gggaaaaatg cttttgctgg acaaggaaaa agattacctg
     6661 ataaagcaga tttctacatt cttgaacagg aaaataaacg actaaaagag gaagacgagt
     6721 gttctacgaa cacaaagatc gatacggatc tccagggatc actcaaaaac tttacgatga
     6781 aggaatcgaa atcaataaac gtgttgtagc tgttcttatg cgtgaaatga atttatgcac
     6841 aagaggattt catcgacgca aatcttctca tggtagagaa aaaacaatca aagaaatggt
     6901 aaaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt gatgctgttt gggtgacgga
     6961 tattacttac attccatgta atgacggtcg cctctatttg tccacctata ttgacttagc
     7021 aactcgtatt ctacgttact atatggtgga ttcacacatg aaaaaagaga ttgtcgtccg
     7081 tccattacaa atttattaag gagaattgcc tacggtaatt cattccgata gaggtagcca
     7141 gcatcggtct tttatttatc aagagctgtt gaccgaccac cacatcacac attcaatgag
     7201 ccaaactggg actcctgttg acaatgcagt aattgaagcg tttcatcgat caatcaaatg
     7261 agaattaatc gactccaaca aatataaaag taaaatagag atgaaggtgt taatccaaga
     7321 ttatttagaa gactactacc caaacaaaag aatccataca aaatttatga tgactccttg
     7381 acaatttgaa gcgaagaaaa ta
//
LOCUS       DACCSK010000122         7267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCSK010000122 DACCSK010000000
VERSION     DACCSK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.125"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            232..1716
                     /gene="lsa"
                     /locus_tag="IVP39_002342"
     CDS             232..1716
                     /gene="lsa"
                     /locus_tag="IVP39_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7587399.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            1770..2573
                     /gene="lnu(B)"
                     /locus_tag="IVP39_002343"
     CDS             1770..2573
                     /gene="lnu(B)"
                     /locus_tag="IVP39_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAP7587400.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            3261..4082
                     /locus_tag="IVP39_002344"
                     /pseudo
     CDS             3261..4082
                     /locus_tag="IVP39_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4225..4449
                     /locus_tag="IVP39_002345"
     CDS             4225..4449
                     /locus_tag="IVP39_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587401.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            4464..5333
                     /locus_tag="IVP39_002346"
     CDS             4464..5333
                     /locus_tag="IVP39_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7587402.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5314..6048
                     /locus_tag="IVP39_002347"
     CDS             5314..6048
                     /locus_tag="IVP39_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7587403.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6081..6989
                     /locus_tag="IVP39_002348"
     CDS             6081..6989
                     /locus_tag="IVP39_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7587404.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            6986..7234
                     /locus_tag="IVP39_002349"
     CDS             6986..7234
                     /locus_tag="IVP39_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP7587405.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         2531 a         1039 c         1510 g         2187 t
ORIGIN      
        1 cttatgttat gtataacaaa aggtaataat agtatttgtt tttgagccgt aagaatttac
       61 tttcttgcgg cttttaatat ttgtaaaaga aaggaagata aacgtgatag acaatgatac
      121 tatgtcattc atgacaaaat ccttgcagag ttggataaca ttatagagta attaatgccg
      181 aaaaccgact ttgcttataa aatttttata atgtaaagga ggaagaaata tatgtcctta
      241 ataaatgttt caaatctaac tttttcatat gaaggaagtt atgacaatat ttttgaaaat
      301 gtaagttttc agatagatac agattggaaa ctcggtttta ttggaagaaa cggacgcggt
      361 aaaactactt tcttaaattt actgcttggc aaatatgcgt attccggcaa tataagttct
      421 acagttaagt ttgagtattt tccttatgat gtggaagata agagtctata tacaattgaa
      481 gtaatgaaga gtatttgtac ggaatttatg gattgggaga tttttcgtga aatatcattg
      541 cttgatgttc aagaagatgc tttatatcgt ccgtttaata cattgtcaaa tggtgagcaa
      601 acgaaggtcc ttcttgcagc tttattcctt acagcgagtt gtttcctgct tattgatgaa
      661 cctacaaacc atcttgacat cgatgcacgt aatgtagtgc aaaactattt gaaacgcaag
      721 aaggggttta ttttggtatc tcatgataga agcttacttg atcaatgtgt tgaccatata
      781 ctatctatca ataaaacgaa tatcgaaatc caaaagggaa attttacttc ttggtgggag
      841 aacaaaacgt tacaagataa ttttgaactg gcagaaaaca agaaactcct taaagaaata
      901 ggaaggttgt cttatgcagc aaaacgtagt tcaaactggt caaataaagt agaaaaaagt
      961 aaatatggaa caacaaattc tggttcaaaa ctggataagg gttatgttgg acataaggct
     1021 gcaaaagcga tgaaacgtgc caaaaatatt gagtcaagac atcaggaagc cgttttacaa
     1081 aaatcagaac tgctccacaa cattgaacaa tatgatgact taaaaatttc accacttgaa
     1141 tttcacaaag agtgcttaat agaagcgaat gatttatcat tgtcttatgg agataaagaa
     1201 gtatgcagta atcttaattt cagagtcaat attggtgata gagttgccat tatcggaaaa
     1261 aatgggagtg gtaagtctag tatcctaaaa ttgattaatg gagatgatat taaatttacc
     1321 ggaaatttta tgctagcaag tggactaaaa atttcttata tttcgcaaga tacttcatat
     1381 ttaaaaggta atctatctga atttgcctat aataataaga tcgatgaaac tctatttaaa
     1441 acgattcttc gtaaactgga ttttaataga gagcagtttg ataagaacat ggtggatttt
     1501 agtgctggtc agaaaaagaa agtactaatt gctaaaagcc tttgtgaaag tgcacatttg
     1561 tatatatggg atgagccatt gaactatatt gatatttttt cacgtatcca aattgaaaaa
     1621 atgattttgg aatattgtcc tacactattg tttgtggagc atgatgatgc tttttgcaat
     1681 aacatttgta cgaaaaatat taatttaggt ttgtagagat tttgagttgc cacaataact
     1741 aaaagatatt tacatgaaag ggtgaagaaa tgttaaaaca aaaagaatta attgcaaacg
     1801 ttaagaatct tactgagtca gatgaacgaa ttacagcttg tatgatgtat ggatcgttta
     1861 ccaaaggaga aggtgaccaa tactctgata tagagttcta tatatttttg aaagatagta
     1921 taacctcgaa ctttgattca tccaactggt tgtttgacgt agctccgtac ttgatgcttt
     1981 ataaaaatga gtacggaaca gaggtagtta tttttgataa tcttatacgt ggggaatttc
     2041 atttcctttc tgaaaaagat atgaacataa tcccctcgtt taaagattca ggttatattc
     2101 ctgatacgaa ggctatgctt atttacgatg aaacagggca attagaaaat tatttatcag
     2161 agataagtgg tgcaagacca aatagactta ctgaagaaaa tgctaatttt ttgttgtgta
     2221 atttctctaa tctatggttg atgggaatca acgttctaaa aagaggagaa tatgctcgtt
     2281 cattagaact cttatcacaa cttcaaaaaa atacactaca acttatacgt atggcagaaa
     2341 aaaatgctga taattggcta aacatgagta aaaaccttga aaaagaaatt agccttgaaa
     2401 attataaaaa atttgcaaag accactgctc gattagataa ggtagaatta tttgaagcct
     2461 ataaaaattc tttgctatta gttatggatt tgcaaagtca ccttattgaa caatacaact
     2521 taaaagttac acatgacatt ttagaaagat tgttgaatta cattagtgaa taggagagta
     2581 acgttattca cagtattggg tactgaaaat taacagcata cctcagtttt tcaaaaaaca
     2641 gcgaagccta tggatatgtt cccgtctacc gatagtgcaa aaagtgtcgt ggatatgttt
     2701 ccgagaacgg acatacacaa atgacattca ttctgtcctc tcaatccatg tggaagcagt
     2761 atgtctgtta acaagaaaat aattattgtt cgtcctgacg agttgttaga ctcgaaaagg
     2821 ataaacatca tttttagaac cggtagccga tttttggcta gcggtttttt gggctctttg
     2881 gcaaatagtg gtgattgaat ctcgcgctat cctatggaaa accgccttgt gtgaggggct
     2941 gcgagaccgc ttcgatccta gaattgctga agcacgaatc tagcgtcact cacatttcat
     3001 cttttgacgt agctgagaca aggtgttttt cttcgatccg ctagtggtta atctcttttt
     3061 ggtgcaaata tggtttagaa tttctttgca tggcttaagg gagtggagaa tcggatatta
     3121 gtaggattta catccacaga taaaaatagg cttgatttta acagcgaaac taaaataccc
     3181 tacttaaatg aagtgataga ttagatgttg ggttgttagg agatgcttaa aaatcctatg
     3241 caatcaatag tttttagtgt ttgttgactt gatccctaaa tcgcctgagt actaaacgtt
     3301 gagtgtgtct gcttactgtc actagccagt aagtgagact gtagttaatc tctcattttc
     3361 cccagtatta tgttgaaaat agccatgaag cgattatttc taaagagtta tttttgcagg
     3421 ctcaagaaga aatttatcgg agaagtaata tctacacagg agaaggcaag aacaaaagaa
     3481 tttatagtag caagtacgct ttaagtgcca tcaccttctg tggatattgt ggcgatattt
     3541 ataggagaac ctattggaat attcatggca gaaaagaatt tgtctggcga tgcgtgacta
     3601 gaatcgagca gggtcccgag gtatgtaaga accgaaccgt aaaagaagat gaactctatg
     3661 gtgcggtaat gaccgcaatt aataagctac ttgctggtgg caacaatatg ataaagacac
     3721 tggaagaaaa tattcatgct gtgattggag aaaccacaga ataccaaatt tcagagatta
     3781 ataccctact agatgaaaaa caaaaagaac taatcaagct ggccaataaa ggtcaagatt
     3841 atgaatacct ggtagatgag atcgatgaga tgcgagataa gcgtcagact cttttagtag
     3901 aagatgcctc cctcagtggc gagaacgagc gcatcaatga gttgattgaa tttatccgca
     3961 agaacaaatt ccgcacctta gaatatgatg ataagcttgt aaggaagata atccagaacg
     4021 ttaaggtcta tgaagaccac ttcgtcatag ccttcaaacc aggtatcgaa atggaaatat
     4081 gaaaaccaga agtaaatagc ccatgactct gaagtagagt tgtgggtttt cttttaattg
     4141 ctgattataa taatatttta atgactattg catttatcaa ccaaatggtt tatgttattc
     4201 ttagcataaa cttggaggtg ctgtatgact acggcagaaa tgattaaaga actgtgtgag
     4261 caaatgaata taagtgtttc cgaacttgct agacgtattg gccagactcc acagaatttt
     4321 aataaaaaat tacaacgaga aacagtaacc ttggatgagt tgaaggccat tgctgatgtg
     4381 ctaggtgtca agtttgtgca ggcatttatt ttaccagatg gagatgaaat aaaaatatct
     4441 aacgaataaa ggaggatgtt tatatggtag gtaatgttat tcaaatagta acagagaaat
     4501 tatcatcttt gccttttata gaaggcattg tattaggggg ctcacgtgca agaagcaccc
     4561 atacagaaaa ttctgatata gatatcggaa tctattacaa ttcagactca tttgacctga
     4621 cagcgattaa tcaaattgct acagagttgg atgatgagaa tagaaacaac cttgttgtac
     4681 ctcccggagc gtggggtgat tgggttaatg gcggtggatg gttagttata aacgggtgtc
     4741 atgttgactt gatattacgt gatataaaac gggtggaaca aataatcaaa gatacggagc
     4801 aaggaattgt tactgccaat tatcagaccg ggcatcccca tggttatata agtgctatgt
     4861 atcgtggaga attagcgatt agtaaaatac tatatgctaa gaacgaaagc ttatgcgaat
     4921 taaaaaagca ggcagaaatt taccctactg ctctaaagaa aagcttgatg aactttttta
     4981 tatttgaagc tgaattctct ttaatgtttg taaaagcaaa tgcgggggca gaggataaat
     5041 attatattgc aggccatgtt tttcgtatta tttcatgctt aaatcaagta ctatttgcat
     5101 gtaataatgc ttattgcatt aacgaaaaga aagctataaa actgcttgaa acttttgaat
     5161 ataaacctga aaaatatgcc gaaagagtta atcatatttt tgaagtactc ggtctttctc
     5221 tttttgaatg ctacgacatg accgagaaac tttataaaga agtgaatgaa attgtatcgg
     5281 agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata tgatgataat
     5341 atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga
     5401 gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta
     5461 ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta
     5521 ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag
     5581 attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta
     5641 atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata
     5701 tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact
     5761 gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg
     5821 gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa
     5881 tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt tataataaat
     5941 cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa
     6001 atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa
     6061 aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt
     6121 tagcagaaca ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta
     6181 atatacctaa agatgaattt caggattatg atattacata ttttgtaagt gatatagaac
     6241 cgtttatatc taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc
     6301 cggaggatat ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg
     6361 atgattacaa taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa
     6421 agggcgataa attaataaag gttctaattg ataaagattg tagaattaaa agggacatag
     6481 ttccgactga tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct
     6541 gcaatgaatt ttggaatgta acaccttatg ttattaaagg attgtgccgt aaggaaattt
     6601 tatttgctat tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat
     6661 ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg
     6721 aaaggtatat atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct
     6781 atgaaaacat atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg
     6841 aggtggcgga aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata
     6901 ccagggacat gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg
     6961 ctgatataga agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga
     7021 tatcgataaa cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa
     7081 cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga
     7141 cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa
     7201 gataatatat cttttaaccc gtggtgctag ttaattttaa tatcctaaag aatatctcaa
     7261 agttcct
//
LOCUS       DACCSK010000123         7149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCSK010000123 DACCSK010000000
VERSION     DACCSK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.126"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..564)
                     /locus_tag="IVP39_002350"
     CDS             complement(<1..564)
                     /locus_tag="IVP39_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7587406.1"
                     /translation="MDQAKSLETACKLLRLSHVLPTLVRKDLDYSFETKEDWLMYLLE
                     KELACREELKTARLLKQAKFPNKRTLEDYEWHDHICLPNATTKDELLSLSFIHQKENA
                     VFVGTPGTGKTHLACGLGQLAAKSGIDTRFWRVSDLVNELEHHWKNNTLEVFKHRFDK
                     VKLIILDEMGYVPFTKEGAELLFQLISD"
     gene            complement(564..1988)
                     /locus_tag="IVP39_002351"
     CDS             complement(564..1988)
                     /locus_tag="IVP39_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP7587407.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPKKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVTQTKTKTEQAVLTD
                     GFLRFSMHYGFETQVCNPRSGNEKGSMENKVGYVRYNFFSSTPIMKDFASFNEALAVE
                     LSHDRERIHYEKHVIINDLWNTEKTDLLALPDTDYPVFKEIEVKVNKYNEIKLDNERI
                     HVPRVRNYSIIYGLLRFDSYQLISTDGEIISEGLRPYMMKKRAIDWQAILPDWRRKSR
                     AMHYSRYWKYLPERIKLFLSHPDWKEQEKRLNQISGLLVTHDMLGIDTHFYDMFSAST
                     TTDAYDIDWHQYDAFLSLTGEVID"
     gene            complement(2383..3225)
                     /locus_tag="IVP39_002352"
     CDS             complement(2383..3225)
                     /locus_tag="IVP39_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7587408.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            3550..3966
                     /locus_tag="IVP39_002353"
     CDS             3550..3966
                     /locus_tag="IVP39_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7587409.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            3963..4433
                     /locus_tag="IVP39_002354"
     CDS             3963..4433
                     /locus_tag="IVP39_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7587410.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            4447..5220
                     /locus_tag="IVP39_002355"
     CDS             4447..5220
                     /locus_tag="IVP39_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7587411.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            5234..6046
                     /locus_tag="IVP39_002356"
     CDS             5234..6046
                     /locus_tag="IVP39_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7587412.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            6100..7065
                     /locus_tag="IVP39_002357"
     CDS             6100..7065
                     /locus_tag="IVP39_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7587413.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
BASE COUNT         2233 a         1243 c         1387 g         2286 t
ORIGIN      
        1 atcgctgatc aattggaaaa gcagttccgc gccttcttta gtgaaaggca cataacccat
       61 ctcatcaaga atgattaatt ttactttatc gaagcgatgc ttgaagacct ctaaggtatt
      121 gtttttccaa tggtgttcca attcgttgac taaatctgat acccgccaaa agcgtgtatc
      181 gatacctgat ttggctgcca gctgacccaa tccacaagct agatgtgttt taccggtacc
      241 gggtgtaccc acaaatacag cattttcttt ctgatgaata aacgatagac tcaataattc
      301 atctttagtc gtcgcatttg gcaaacatat atgatcatgc cattcgtaat cctctaaggt
      361 acgtttattt gggaactttg cttgttttag gagtcgggca gttttcaatt cttcacggca
      421 agccaactcc ttttccagaa gatacatcaa ccaatcttct ttggtttcaa agctgtaatc
      481 taagtctttg cgaaccaaag tgggaagaac atgggaaagt ctcagcaact tacaggcagt
      541 ttctaaactt tttgcttgat ccactagtca atgacctccc ctgttagaga caagaaagca
      601 tcgtattggt gccaatcaat atcatacgca tcggtagtag tcgaggcact aaacatgtcg
      661 taaaaatgtg tatcaatccc taacatatca tgtgtcacca ataaacctga gatttgattt
      721 aaacgcttct cctgttcttt ccaatctgga tgactgagaa ataacttgat tcgttcgggc
      781 aaatacttcc aatagcgaga gtaatgcatg gcacgcgatt ttctgcgcca atcaggaaga
      841 attgcttgcc aatcgattgc tcgttttttc atcatataag gacgaagtcc ttctgaaata
      901 atctcaccat cagtgctgat tagctgatag gaatcaaaac gaagcagccc ataaataatt
      961 gaataattgc gtacacgtgg aacgtggatc cgttcgttat ctaatttaat ttcgttgtac
     1021 ttattgactt tgacttcgat ttctttaaat actgggtaat cggtgtccgg caaagctagc
     1081 aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc gtaatggatt
     1141 cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc gaaatccttc
     1201 ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt ttccatacta
     1261 cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata gtgcatagaa
     1321 aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt ctgagtaacg
     1381 gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg gctgaagagt
     1441 tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac ggcgaatcct
     1501 gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc tccatcgtga
     1561 caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc aagacgttca
     1621 aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg aatgaataca
     1681 cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt cattgcttca
     1741 gcagtcctac gctttttctt tggcagctta gcatcttctg aaagccatag ggaaacgatg
     1801 cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg ttgcggcagc
     1861 aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc ctttgcaata
     1921 tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat atcggtcatt
     1981 gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta gaggaatttc
     2041 aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg tgtagttttt
     2101 cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt ccacttgaaa
     2161 aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac acagaaatat
     2221 aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac aaaaaggtag
     2281 ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga ggggatattt
     2341 catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta tctctaagtg
     2401 ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata tgttgaaggc
     2461 ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat tcaatttatt
     2521 tccaattttc attacctctt gctcaggtag acctaaatta tttatataag gtatgccaat
     2581 catatggaag ccaccgatta ggtattttat gggtttagtc ggaaatgttt caagagcaat
     2641 ttccatcata tcgataatgc catgatgact acagccagta aagacaatca gaccgtcttc
     2701 ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt tatcatccat
     2761 ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga tatggaagct
     2821 cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt taaggatttc
     2881 atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat ggggtaaagt
     2941 tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta acccgccggt
     3001 gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag tttcccccat
     3061 tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat atttcttccc
     3121 tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat ttattcttga
     3181 gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa cgtgtattca
     3241 tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa tattaatgga
     3301 cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg cgggggattg
     3361 gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa atacccacac
     3421 tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga aaaaccttta
     3481 attgacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt aaggatagga
     3541 ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca aggattaaag
     3601 caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc agcatttaga
     3661 gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat agatatagaa
     3721 gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat gctcactatt
     3781 attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt agttatttct
     3841 attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat tagagagagt
     3901 caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg aggagatgac
     3961 ctatgattaa attagtacgt attgaccatc gtttgttgca cggacaggta gtattttctt
     4021 ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca gcgaatgatg
     4081 aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta acaatttttt
     4141 cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa aatatcatgc
     4201 ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct aaagtacaag
     4261 aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt aatgccattt
     4321 tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc attaaacaat
     4381 ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta taaaggagga
     4441 atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg tattttagac
     4501 tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac gcttgttggt
     4561 ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga attgatgtcc
     4621 ttaggattag taaatatcgg agctgctgca ccacctgata tgaatatggc atcaattatt
     4681 gctacagcct ttgccatttt atcaaatgcg aacgcagaag cagcattaac gattgccatt
     4741 ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt gtcaaatttg
     4801 actcacgttg cggatcgctt tatcgaacaa gggaaattta gccaagcaag gagaattcat
     4861 atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt tctagcaatt
     4921 tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg gctaacaaat
     4981 ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt attacaaacg
     5041 atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac tgcttattct
     5101 ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt catggttcaa
     5161 gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga tgacttataa
     5221 ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc agttcttatg
     5281 gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca tgggttttat
     5341 gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg aagcaatgaa
     5401 gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa caagtgcatt
     5461 tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag aaaactttaa
     5521 tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg gtgttggtga
     5581 ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta atttagccca
     5641 acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac cttccttttt
     5701 agttacttac tatggtggga aaattggcta taacatgggg aataaatact tgtctaaact
     5761 gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg ggttgatggt
     5821 cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa tgggtaaagc
     5881 ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat taggattaac
     5941 cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt tggcaatctg
     6001 tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga ataaccaata
     6061 aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa agaaattgtt
     6121 aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg tggcgcttca
     6181 aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca gttacggatt
     6241 agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga tgagtcaact
     6301 gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc taacgcagta
     6361 gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc accgttaaca
     6421 aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc taaattagaa
     6481 aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga aggttatgac
     6541 cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca aaaaagtgca
     6601 gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga aaaagttatt
     6661 tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat ttgcttgatg
     6721 atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt ccacggacca
     6781 ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg aaaaactaga
     6841 aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac agaagttgta
     6901 gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga ctactttaac
     6961 ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga agcaagagaa
     7021 catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat agatcaaact
     7081 aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt ttatacatat
     7141 ggtaaaata
//
LOCUS       DACCSK010000124         7002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCSK010000124 DACCSK010000000
VERSION     DACCSK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.127"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(177..863)
                     /locus_tag="IVP39_002358"
     CDS             complement(177..863)
                     /locus_tag="IVP39_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7587414.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(999..1193)
                     /locus_tag="IVP39_002359"
     CDS             complement(999..1193)
                     /locus_tag="IVP39_002359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587415.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1243..1683)
                     /locus_tag="IVP39_002360"
     CDS             complement(1243..1683)
                     /locus_tag="IVP39_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7587416.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1687..2532)
                     /locus_tag="IVP39_002361"
     CDS             complement(1687..2532)
                     /locus_tag="IVP39_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7587417.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2681..4099
                     /gene="pepV"
                     /locus_tag="IVP39_002362"
     CDS             2681..4099
                     /gene="pepV"
                     /locus_tag="IVP39_002362"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7587418.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4156..5103)
                     /locus_tag="IVP39_002363"
     CDS             complement(4156..5103)
                     /locus_tag="IVP39_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7587419.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5326..5676)
                     /locus_tag="IVP39_002364"
     CDS             complement(5326..5676)
                     /locus_tag="IVP39_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7587420.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5876..6955
                     /gene="pepA"
                     /locus_tag="IVP39_002365"
     CDS             5876..6955
                     /gene="pepA"
                     /locus_tag="IVP39_002365"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7587421.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
BASE COUNT         2145 a         1369 c         1361 g         2127 t
ORIGIN      
        1 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
       61 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
      121 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattctaa aacctcttat
      181 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
      241 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
      301 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
      361 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
      421 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
      481 aaatcaaaaa aagctttttt attttgagcg atcactgcct gatcggtttg ttctagtatt
      541 ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg caggatcggt
      601 ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc gatttttgaa
      661 acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag ttggatcagt
      721 gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt atcggagatt
      781 ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc ataagcttgc
      841 tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa tctattataa
      901 cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa agccctgata
      961 ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg tacctcttgg
     1021 atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt tttgtctttg
     1081 aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt atcgatgtaa
     1141 ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt cattatacga
     1201 actactcgac acaatacaag aaaaatgctt agacggtctt tattaagaat ttacatcatc
     1261 caaagaagcg tacgcttgga ataactgatc taactccgga ttgactttgc tcagcttcac
     1321 taatcgctgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag tttttaattt
     1381 tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgtccccg tatacttttg
     1441 gatagttgga tggatgctca ctttttctcc gtaatggatc atttctttat agctgcaatt
     1501 tacttccaga acggggatga tgataccaga ttcttcgatt tttttatagg gaaaccctag
     1561 atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc tgtgatgaat
     1621 aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg taaatttctc
     1681 catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc acttcggaag
     1741 tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct ggtctccaat
     1801 gaaactatga agataaacag ctgctgcaat cgtttggtca ttttgaggaa actgggctaa
     1861 aaatcctgta atcatcccag ctaaagtgtc acccatgccg cctgttgcca tagcagcaga
     1921 acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat gacttttcaa
     1981 aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc gttgttcttg
     2041 tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta cagtttgttc
     2101 gggatagggt agcaaaagat gattagcagc aagtaaactt aaagcagagc catcaatcac
     2161 cagccattgt tcttttgatt ggttttctag tacttgtttg aaacgttcca aactcttaga
     2221 agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta cggaaagaaa
     2281 ctggtcagta tccgtccaat ctacaatcat tgcttctggc agatgactgt gaagagcact
     2341 ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccgctgtgta cggcagctaa
     2401 agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa cgatacgtcc
     2461 aaatgttcct ttatgtgaaa tttttggacg aggtgtgatg actcttttca ctagttgttc
     2521 gtttatcagc atcgaaaaac ctccttttac tcattatagc gtatattttt tttagagaaa
     2581 aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta atttgtggta
     2641 tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg attggcaaaa
     2701 agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt tacgtgtcaa
     2761 cagcgagcgc gatgactcaa aagtaacacc agatgcacca tttggacctg gacctcgcga
     2821 cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa aaaatgtcga
     2881 taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga tatttggaca
     2941 catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac cagtaattaa
     3001 ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggcccaagta tggcagctta
     3061 ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag ttcgcttcgt
     3121 agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg aacatgaaga
     3181 agagccagat tttggtttct caccagatgc tgaatttccg atcatcaatg gtgaaaaagg
     3241 aaatgtttct ttagctttac gattcaaagg cgataatgca ggagattatg tcttgaaatc
     3301 ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag cacttcaagt
     3361 accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca tcgaagcaaa
     3421 tccagtaagc ggaacgattg aagcagacaa tacgtatgta aaaatcgaat tagttggtaa
     3481 aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct tggcattgtt
     3541 cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag cagctgctta
     3601 tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg aaaaaatggg
     3661 caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag aggaagcgaa
     3721 cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat tgcaaagtga
     3781 catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc gcgtaatgga
     3841 accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag tctatgaaga
     3901 ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg gacgtttgtt
     3961 gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa tgcatcaagc
     4021 aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg cagatgccat
     4081 ctatcgtttg gcgaaataat taattaaaaa gaggatcaag aattgcaaat tatttttgca
     4141 gtcttgatcc tttttttact ttctactgtg gtattcatga taaacatgat atagattcaa
     4201 ttcatattct ttcttagcgt tcgtgacgac tgtatccaaa atcaaccctg ttactaatga
     4261 aagaagtgct aaagtcatca atccagtact taaaatcgca gaagggatgc gtgtaataaa
     4321 gcttgtcagc atatattcgc gaatcacagg aatccccgtg accaatccaa ataaaaagaa
     4381 gaagatggtc caaatcccaa agaaaagcaa aggcttataa tctttaaaca ttttaataat
     4441 catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc tgccttcagg
     4501 acgatcacga taatcgattg ggatttcaac gaacttgaat tttttatcta aagcatggat
     4561 cgtcaattcc gtttcgattt gaaaaccaga actcataata ggaaaacttt tgacaaagat
     4621 acgattgaat cctcgatagc cggtcatgac atcaagaatt tttgttttat acattctagt
     4681 aattgtattt ctaacaaggt tatttccaaa atcgtggaaa ggtctcttat tctcatcaaa
     4741 gtaggttcca tttgataggc gatcaccaat gaccatatct gcttcaccag aacgtaattt
     4801 ctcgagcaaa ccatgtacag cttctgcagg ataagtatca tcgccatcta ccattaaata
     4861 atagtcggca tcgatatcaa agaacatttg gcggatcaca tttccttttc cttgacgagg
     4921 ttcttttttt acgattgctc caccttgaac ggccaattcg tatgttcgat cggtcgaatt
     4981 attatcatag acataaatat ctgcttctgg taattctcgt ttaaagtctg caatgactgt
     5041 tgaaattgtt gcttcctcat tgtaacaagg aatcagaaca gcaatttttt cttgttgttc
     5101 catttttcac tctccttaac tatagaaaac aaaagctaaa ttaaaaatac ctcataatca
     5161 aaggaaatgc tagtttttcc cgctaaatat catttattta aaaagaaaat actggtatat
     5221 atgtttggaa agctctatct aaaaaaccac ccactacaaa agaaggacat gatacctctc
     5281 ttttgaatag tgggtgcgta ttcgttctct cacttttttg acgtattaat ctgttgcacc
     5341 tttttttctt gaaatttcta atacggttcc tgtatgagca tctgctaaaa attcataata
     5401 aacagtttcg ccatcttcta ttcgagaaag acctccccga taagcactcg accgaacggc
     5461 gaatttccta ataggttcct tttcaaaaga gatccaagaa ccttcgattg caccttcttt
     5521 tagaaaagat gacttgatat tttcaagtac ttggtctgct gacaatactt ttttgcgatg
     5581 ataccaaatc gttgaagcta ccccgctcaa taatcctaga ccgattccaa ctgctaaccc
     5641 gcctttaaaa tagcttagtt cgctttcttc atacatgtgg acatacctcc ttcttctcac
     5701 ttgttactcc ttgtgcttat tttaccacac tctcatattt tctttgaaat aaataaccgc
     5761 taccagaaaa aataagctat aataaatacg atcggtctgc agaaaaaatc atattttatc
     5821 cgcagatcaa tataagtctg agaggagttt agtctgaaat ttagacgaat agcatatgga
     5881 agaaaaaaca tttcaacgaa tcaaagagtt aacagaatta caaggaacaa gcggttttga
     5941 acaagatgtt cgcgcttata tggaaagtca tatggaaccg ctggtagatg agttacagtt
     6001 agatggtttg ggcggcattt ttggtctgcg ccatcatgaa gaagcggatg caccccgtgt
     6061 aatggtcgca gcacatatgg atgaagtcgg attcatgctg acacaaatcc aagaaaacgg
     6121 gctttttaaa gtcgtacctc taggcggctg gaatccttac gttgtttctg cacagcgttt
     6181 tacacttaaa acctctactg gtaagaatta tccatgtatt tcttcttccg ttcccccgca
     6241 tttattgcga ggaacaagcg gacaaaaatc agtagaagta accgatattt tgttcgatgc
     6301 tgggtttgaa tcaagagaag aggcaatgag ttacggtgtc ctcccaggag acacaatcgt
     6361 tccttacgct gaaacgatca aaacagccaa cggaaagaat atcatcagca aatcatggga
     6421 caaccgctat ggctgtacaa tggtattaga agctttagaa gcgttgcaaa atgaaacatt
     6481 aggtcataca ttgattgcag gcgcgaatgt ccaggaagaa gtaggcttgc gtggttctaa
     6541 agcttctgtc aataaattta agccagatct gttctttgca gtagactgct ctgcagcaga
     6601 tgatacagtg acgaaaaatg gcacattcgg acatttagga gaaggtacat tgatgcgtat
     6661 ccaagatcca ggtttgatca tgcttcctcg tttgcgcgag tacttgctgg atatcgctga
     6721 aacaaacaat atcccttatc aatactttgt ttcaaaaggc ggtacagatg caggtgctgc
     6781 tcatacacaa aacgaaggaa tcccaagtac agtgattggc gtagtgggac gttatatcca
     6841 tacgcatcaa acgatgttca gtattcgcga ttttgaagct gcacgagaaa tgttgattca
     6901 aacactgaaa ggtttggata aatcaacagt gaatacgatc gtatacggaa aataatgcga
     6961 tagtgaaact ataaaatctg gcaaaagatt gcttttgcca ga
//
LOCUS       DACCSK010000125         6902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCSK010000125 DACCSK010000000
VERSION     DACCSK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.128"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IVP39_002366"
     CDS             <1..848
                     /locus_tag="IVP39_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP7587422.1"
                     /translation="RYSKTDTGHTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLG
                     IPARWTKGFTNGLRTGTDNQGNEEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPM
                     GSQPVAETTETVQPAIEQPNESENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVC
                     LSVLLFFLLLFRKSLRWTSYLIYLHFFTSTNTIAYQKLLNPLETLVPRKPAESLQEYS
                     LRIEQEFKLDLRFIRLTTQYEAEIYGRQADPVKFDKKTYLEITHIIRKTVYKKILTKG
                     SRFKK"
     gene            complement(960..3131)
                     /gene="copB"
                     /locus_tag="IVP39_002367"
     CDS             complement(960..3131)
                     /gene="copB"
                     /locus_tag="IVP39_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7587423.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(3151..5337)
                     /locus_tag="IVP39_002368"
     CDS             complement(3151..5337)
                     /locus_tag="IVP39_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7587424.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTIIGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(5337..5546)
                     /gene="copZ"
                     /locus_tag="IVP39_002369"
     CDS             complement(5337..5546)
                     /gene="copZ"
                     /locus_tag="IVP39_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7587425.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINRLGYKAEVI"
     gene            complement(5559..5999)
                     /locus_tag="IVP39_002370"
     CDS             complement(5559..5999)
                     /locus_tag="IVP39_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7587426.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(6073..6612)
                     /locus_tag="IVP39_002371"
     CDS             complement(6073..6612)
                     /locus_tag="IVP39_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP7587427.1"
                     /translation="MKIIVIGQSGSGKSTLARNIKEITGFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1858 a         1539 c         1213 g         2292 t
ORIGIN      
        1 tccgctattc caaaacggat accgggcata ctccgaaaga acacgattat gtggattatt
       61 tcctctttga ttcaaaagtc ggctattgtg acaacttttc aacttctatg gtcgtccttc
      121 ttcgaacact tggtatccca gctcgctgga caaaaggttt tacaaatggg ctcagaacag
      181 gcacagataa tcaagggaat gaggaataca gcattctcaa tagtcatgct cattcttggc
      241 cagaggtata ctttcctgga tttggctggc ttccatttga accaacccct tcatttgcca
      301 atccaatggg ttcacaacca gttgctgaaa caaccgaaac cgtccagcct gctatcgaac
      361 aaccaaatga aagcgaaaat atggcagaaa ctatttccag tgagagatct gaggtcattc
      421 catcagaaga aacaacactc acaccagctt ataaaatcgt cttattcagc cttggagtgt
      481 gtttatccgt cttgttgttc tttctattgt tattcagaaa atctctgcgc tggacaagtt
      541 atcttatcta tcttcatttt tttacttcta ctaatacgat tgcatatcaa aaacttctta
      601 accctttgga aacattggta ccgcgcaaac ccgcagaatc tttgcaggaa tatagcctgc
      661 gaatagaaca agaattcaag ctagatctgc gtttcattcg attaactact caatatgaag
      721 cagaaatata tggacgacaa gcagatccgg taaaattcga taaaaaaacc tatttagaaa
      781 taacgcatat aataagaaaa acggtataca aaaaaattct gacaaaaggg agccgcttca
      841 aaaaataaga taaaatttct ggagaatcta cttctggaat gccgagaacc agctgattga
      901 gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg tcacaacctt ttttcattat
      961 tatttgattt tcaatgtcaa agcatttaga gctacaacta ccgtacttaa cgacatcaag
     1021 acagcaccaa ctgctggact taatatgata ccaattggcg caagaattcc ggctgctagc
     1081 ggaattgcaa gaatattgta acctgccccc caccagaggt tctggatcat tttctttcgc
     1141 gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg gatcgctgtt tgtcaatacg
     1201 acatccgcag aatcgattgc gatatctgtc cctgcaccga ttgccatccc gattgaagca
     1261 cgggctaaac ttggcgcatc attgatccca tcacccacca tgattacttt tttcccttgg
     1321 tcaaggtatt tctggacaac agcttcttta tcatctggta ataaaccgcc ataatattct
     1381 tcgatcccta aataatctgc tactgcttgg gcagcgtttt tattgtcacc agtaagcatg
     1441 acaggagtga tttttctttc tttcaatgtt cggataaatt cttttgcttc tgtttttact
     1501 acgtcaccta aagcaacgat tgcagctaga tgattttcta aaatcaggaa gctgatggta
     1561 tttccttgtt cttggtaagg tttcaataat gcttgttcta cgtttaatcc gagacgctca
     1621 acttcttttt cattaactat tttcacttgt tgttttttga ctgttgcctc tagcccgact
     1681 cctgaaagat tttttagatt ttcagcttga taaggcttga tttcatgttc tttcaaataa
     1741 ttcataatgc caacagccaa tggatgattg gcattcgctt ccaaggctcc tagatattga
     1801 aggatttcat ttttattgaa cgcatcatca agtacttcga cacccgttac agtaaatttt
     1861 ccttctgtta gtgttcctgt tttgtccagc atgatcacat ctagatcatt ggcttgctcc
     1921 agtgcattgc ggtttttcaa aagtaatccg tttttagctg ctattgatgt ggaacgagca
     1981 acaactaatg ggatagctaa acctagagca tgaggacagg cgatgataaa gactgttacc
     2041 atacgttcta atgcattagg aagatctgtc aagaacaacc aggcgatgaa tgccaggata
     2101 cccacaatta atgcgacata aaacagccat tttgccactt tgtcagataa tgactctagc
     2161 ttggattttt ctccttgtgc ttttcgcacc atttccataa ccttcgctag ataaccattt
     2221 tcaccagttc cagttacttc gatttcgatc gttccatccc cattgataga accgccaatc
     2281 actttgtcat tggcttgttt tttgattcct cttgattcgc cagtcacagc tgattcgtca
     2341 acaattgttt cacctttcaa gattacacca tctgttggca ttttatcacc agaccggacg
     2401 atcaaacgat caccttcgtt tacttctttc aatgaaactg tctcttcttt tccatctttc
     2461 gtcaagcgtt tcactgattc tggcagtaat tctgctagtt tttgtaaagc attgctggca
     2521 ttcgacacag cattcatttc aatccagtga cctagtagca taataacgat caaagtagcc
     2581 aattcccaga agaaatccat gacgtgtgta tgtggattga gcagatttgc gataaacgaa
     2641 tagacactat aaatatatgc aactgtaatc cccatcgcga tcagtgtcat catagcagga
     2701 cttttttgtt tcaattccat cttagctcca cttaaaaatg gctgaccgcc atagataaat
     2761 agtatagttg ctaagatcaa tacgagccaa tcagaaccag gaaacgttac ttgaaatgga
     2821 aattccattc ccatcattgg tgaaaaaaga atgattggga tagctaggat aagcgatagc
     2881 caaaattttt gtttgaaatt ccccatgtgc atggaatgat ccatgccgcc gtgttcgtga
     2941 tccatgtgca tcgaatggtc catttgtttg ctgtttgcta tatctccatg gttatgtccc
     3001 atgtgcatgg aatgatccat ctgactatgg tcatgtttca tctccatctg tttttcatga
     3061 gattctgtac tttctttgtt tactacttct tttttatgct catgtttttt ctccatatcg
     3121 ttcccattca tttttactcc atcccttctc ttactttttc atgtgtcgat ttaagctcag
     3181 ggagttcaac aatacactga cggaactaaa cgccatcgct cctcctgcaa tgattggatt
     3241 caaaaagcct agtgcggcaa aaggaatacc aatcgtatta tagacaaaag cccagaaaag
     3301 attttgtttg atttttctca atgtcaacct agacaactcg attgtttgtg caatggatgc
     3361 caaactgcta ttcatcagcg tcacatcggc tgtttccatc gcaatgtccg ttccacttcc
     3421 cattgccata ccgacatcag ctaatgctaa ggccggtgca tcattgattc catcgcctgc
     3481 cataccgact tttttgccat ctttctgaag tttttctacg taggctgctt tatcctctgg
     3541 aagtacttca gcaaagatat gctttggatc aataccaact tgtttaccaa tcgtttcggc
     3601 tgccagtttg ttgtctcctg tcagcataaa aacatctaat cctttattct ggagctgctt
     3661 gattgcttgt ttggcttcta gtttgatttg atcagccacg gcaatcaacc cgatcacttg
     3721 cttttcatca gctaaaaaca tgactgtttt tccctgctgt tctaattcta acgcgtgttc
     3781 ttggtattca tcaaatgata ggttcaattc aattaatcgt ttacgtgtac ccgcaaaata
     3841 gcgtactccg ataatcgtac cgctgattcc agcacctggg tgagctgtaa aatcaatcat
     3901 atcgtatgtt gctgcttctt gcagttttcc gtattccacg attgctttac ctagtggatg
     3961 ttcagaagct tgttctaaag tataaaaaat agataggact tctttagaac cgactagatc
     4021 tgtaactttg ggctttcctt ctgtaatcgt tccagtttta tccaaaacaa tgctgtctaa
     4081 gtgagcagct gcttctaatg cttctccgcc tttaattaaa attccatttc gtgcaccaac
     4141 acccgttcct accataatag ctgttggagt cgctaaacct aatgcacatg gacacgcaat
     4201 gactaataca gagacactat ggatcaatgc gagttgccaa tcaccagtga tcaaacctgt
     4261 agctatgagc gtgataaacg cgatgactaa tacgatcgga acaaaaatcc ctgagatccg
     4321 atctgcgatt ttttggattg gggctttgct cccttgagca tcttccacca tttgaatgat
     4381 ttgcgctaat actgtctgct tgccgacttg tgtaacttcc atgtgaagta gaccattcgt
     4441 attgattgtc ccgccaaaga gcgtgtcatc cggattcttt tctacaggaa gattttcacc
     4501 agtaagcata ctttcatcta tcgcactgct tcctgaaata attcgaccat ctgctggaat
     4561 ctgctcacca ggacggatga ctagttgatc accaacgact acttcttcaa tcgctaacgt
     4621 ttcttctttc ccgtttctta tcacttgggc ggtttttgtt tgaagagaca tcaattgttt
     4681 gattgcatta cctgttttcg tttttgctgt atgttccaaa tatttgccga gcaaaatcag
     4741 cgtgatgatc atactgctgc tttcaaaata aagatcttgg gggtgtccac ggaaaaatcc
     4801 gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt gctaccaata catccatatt
     4861 tggtgctttc gtcttgattg catggtacgc acctttataa aaacgagcac cgacataaaa
     4921 ttggacagga gcagacagca ctaactggac gattggaaaa tgaaaaaagt gaacaatcgc
     4981 cgcatggctt cctaacatca tagcaatcat tgacaacatc aacggcaaag tcaaaaccgt
     5041 actcaatata agatcgaata gcattttttt caaatacgct tgcttttctt ctgcaatttt
     5101 ttgtttgtgg gcttcatcgt acaaaattgc accatagccg atgttttcta cgctttggat
     5161 taatttttca gtggtcgtcc cttcatattt gacacttgct ttttcagtgg ccaagttgac
     5221 tgtggcattg accacaccag gttgtccgtt cagctctttt tcgatcctag cagaacaatt
     5281 tgcacaagtc atacctgtaa tgacgaacgt atccattttg gtttctgctg ttgccatcaa
     5341 atcacctctg ctttataacc caatctattg attgcttgac aaatcttttc ggattgaact
     5401 tttgtttcat caaacttgac cgtaccgttt gcttttttca aatttacttt cactttttgc
     5461 acgccgtcaa gtgcgctcac tgtttcttct actcttgctg cacaatgatt gcagctcata
     5521 ccttcgattg aaaattgttg tttcattttt tcttcctcct attgatgttg tttacattca
     5581 cattgacctg ggatacagtt acattcgatc gtatctactg gttgcttttg ttccagttgt
     5641 ttcaaaatct cattgatatc ttcttgagtc aaagtggctt cttctactag atccgcaatc
     5701 gtctctccaa tcctttttgc acagatatgg gaaaaaagat tttccgtcgc actgcgaact
     5761 gtttccatct ccgagacagc tggatgataa atgaattttt ttccttcttg ttctgtccag
     5821 atcacttctt tttttaccaa acgacccagc aaagttttga tcgttgctgg tttccaatcc
     5881 atcgaatcag ctaagatctg cgtgatttct tgggctgtcg tctgtccaag tgtccaaata
     5941 acacgcatga tctcccactc tgaatcactg atctttactg gcgttttgac tttttccatt
     6001 tatttcacac ctttcgttta caaatgtaat cattatctta tgaatacaga ttacacttgt
     6061 aaacaaaaac tgtcaattat ttttgctctc tattttcaat ttagttaaaa attcttgttt
     6121 ctctcgtctt gttttaagga tgatcagttt atttcttgca ttagctactt ggatcagctg
     6181 ctgtattttt ggttcattgt tttcttcaaa atgccagata aaagataaaa attctaagta
     6241 ttccctgttt agtttctccg aaaatgattc aggcatatct ggccgagatt ttttgtttat
     6301 ctttcttttt agagaacgcc aaactactcg gtagatggcg agataacgcg gtactttaag
     6361 ccaaatgatc ttatctgctt ctttaatcct ttcctctaac gtagaagtat aatttccttc
     6421 aacgatccat gacggattag ataccataaa cgattgctgt tcttgcaaaa accacttttt
     6481 tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct agcggcagta gcggaaagcc
     6541 ggtgatttct ttgatattcc ttgcgagggt cgattttccc gacccacttt gtccaatcac
     6601 gatgatcttc actcttctcc ctcacttctc tttggaattt ggttcgatca taccacttat
     6661 aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat
     6721 aaatggtgtt caatcagctg accttcagca ttagctcaaa aagcggcaaa atatgaggag
     6781 ctatttttcc gtttgtttct ctaatactca ggtcaaatcg ctgattttac aacctcttcc
     6841 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc cagccaaacg aaaaatatga
     6901 gg
//
LOCUS       DACCSK010000126         6878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCSK010000126 DACCSK010000000
VERSION     DACCSK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.129"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..235
                     /locus_tag="IVP39_002372"
     CDS             <1..235
                     /locus_tag="IVP39_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP7587428.1"
                     /translation="FADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKG
                     KEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTV"
     gene            complement(953..4102)
                     /locus_tag="IVP39_002373"
     CDS             complement(953..4102)
                     /locus_tag="IVP39_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7587429.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(4115..5314)
                     /locus_tag="IVP39_002374"
     CDS             complement(4115..5314)
                     /locus_tag="IVP39_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7587430.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5304..6842)
                     /locus_tag="IVP39_002375"
     CDS             complement(5304..6842)
                     /locus_tag="IVP39_002375"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7587431.1"
                     /translation="MDASEYKNYLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQ
                     TMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQ
                     LASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYE
                     FLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVR
                     NYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTF
                     DAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIV
                     LPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVL
                     FIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPR
                     YVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEMWLQGALEVF
                     KHEQ"
BASE COUNT         1924 a         1407 c         1148 g         2399 t
ORIGIN      
        1 atttgcggat gcaaaagaaa agcatgggat gcgttggacc aagtatcgag gcttggaaaa
       61 agtcgcgacg catacgatgc tagtgtttgc tgcaatgaat ttaaagaaat tagccacctg
      121 gctatggaaa ggcaaggagc ctttattttt ttgttcaaaa attcgaaacg aagtagataa
      181 aaagctgttc caagctcgag ttacgagttt ggaacagctt ttgtctacag tctgaggact
      241 aatacaggta gtcctctttt ttgttatttt gatattctaa atcatttttt attggattat
      301 tcccttgttc gatatagacc caaattgatc tatcaataat tatcaaactg agtcatatag
      361 agacaaaaat ttcaaaaatg acctgttaaa tggtcatcga atatcattta aaatcaatac
      421 gacggagtca gacacggagt caaaacaagc ccgctgagag taaaaaatat atacagtccc
      481 ataggtaaag tgccacagga ctgtatattt ttttatttga gattacctga aatgattcca
      541 tttacttttt atctcatttt ttcctactag ttagtatcaa atactctcct attttctcaa
      601 taaactattt tgggttacat ggtatctgaa acagctaaaa ccttgtcatt acagcaaaaa
      661 atcactttat ctccgtttca tttcagatac atttcactta agcggattcc aaaaaaacaa
      721 aagaaaaagg aacccctagt tagcctatgc tttttattgg ttcctctcaa aacacggtca
      781 tataacaacg gggtggggta aatgaatgaa aaaggtggag gaaacattat tcaatagctg
      841 tcttaaatac ttcgttataa aatgtgaaca aataaaagac atctatccct attttctaca
      901 cttccacttt actaccatct ttgaatcgta caattttctt aactcttctc gtttacctca
      961 attcattttc aagtggaatc agctcatcat ctaacatttt acgccatgct cgcttcattt
     1021 cttgtgggta tttaaatggc ttcgcttctg gatggttggc tttatatcct tcgtaatcct
     1081 tactgttgat aattgctttc atattaggaa ttggatcttc acctggcata tattgcagat
     1141 tagaagcatg taattctttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat
     1201 catcactcgc ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttactatcta
     1261 aagtccccac atcaaacgca tttaaaattt gtcggtaaac ttctttgact ccttctgttt
     1321 tattcaattt agccaaagct ttttcaattt cttctcgtgc atctgaacta ttcgccgcat
     1381 aagttcctag taattgatca atataagtag aatcaatatc atacaacttc gtggtatttt
     1441 cactataaaa ttcgatcgtg gaaaaatctt ctccctcttt ttctggttct tcctctatta
     1501 atgaaccctt cacggtctcg tacacgccaa tttgttcttc caaaagttga atttgattgt
     1561 tcaaggttga agatttcacc atgtcatcat tgtaatcgtc ataagtcact aacgattcat
     1621 aagcacgatt caattcttgg aatgctttga cgtactcgat acggatttca agtggcgtat
     1681 gttcatcaat ggattccatg gatgaaacct cttttaactt accatatgct tgcttgaatc
     1741 tttttttgga ttcctgataa cttggataaa tcaaacccga ttcttctttt gcttctgaat
     1801 acagttttgt cgcatcctcg acattcttct ccattgtggc tggctttcgg aaagtaacaa
     1861 ttagtccttt tgttttttct ggatacgtac ggttcgtacg tgaaaaagct tggatcaatc
     1921 ctgcatactc tagattacga tccacaaata gggtttgaat cgtaggtgcg tcaaaacctg
     1981 tcaatagacg atccactacg atcactaaat caatttgttt cccaaattgt ttgaattccg
     2041 ctctttttcg tgccaaacga ttattgatgt ctccgttata acgctcaatg tctgctaatg
     2101 accaggaagt atcataatac tgattatatt cctcaataat tttttgcatt tcttcttgtt
     2161 gggcacttga atctcggcta ttttcttcta atgaataggt aatagcgatt cgtggaaaat
     2221 caggatcctc catcgttcgt ccttcacgaa tgagctgatt gggaaactct tttgttaacc
     2281 aatcaggctc tttcgtcatc tctttgatgg cccgataata ctttttggcc atgtcaatgg
     2341 aactcgtggt taagattgca gatttcgttg gcctgccatt tcgaaaatca aattttgtat
     2401 aggcattatc tggacggaac attttatgaa tgactttttg gatatgttca tctgcttcaa
     2461 aaacagctgg gtccaaataa ctttcttttt tgacaggttc catttgatca atcatacgat
     2521 tgatctgttc tgatgaatac tctgcatatg tttctacttc ttttaatttc cgatagattt
     2581 tattctctag tgaagtcggc tccacggtgt tttcatgctc tacttgaaat cctaagacag
     2641 agccatcttc tagcgcattc ttaatggtat aggtatgcaa gacctctcca tactgatcat
     2701 gggttgtacg tgccaattgc cctttggctt gtttccgatt ctcaggaaaa atcggtgtac
     2761 ctgtaaagcc aaaccaagtg gatttcggaa acattttttt gatttcttcc atattttcag
     2821 cacttaacgc tcgatgacac tcatctacta caaacacgat atgttggccc attaattttt
     2881 ggaagcgatt ggttcctttt ttctcttctt gtttctttgc atatttcaac gccgcatcta
     2941 gtttttgacg ggttgtaatg attacaacat ttgcattggc atctgccaac agggtttcac
     3001 ttaattcttt ggcacttccc gttccaacga tcaagctatt cgctttggcg ttcccggaag
     3061 aaatcccagt attaaattcc gaagcaaatt ttgtaaactc tgtggtggtt tgattatcta
     3121 aatctttgcg gtccactaac atgatcgttc gatcgatccc cgattttcgt gctaaaagct
     3181 tagtagatac aaaactagtc aacgtctttc ctgaaccagt cgcatgccac acataccctg
     3241 attgatgttt atttgcggca ataaacaagg cttgaatcgc atgtacttga taaggatgta
     3301 acaccatgag ggttttatta tcttgatctt cactgacgat tgtataatcc gcaatcaaac
     3361 gatgagcatc cgggatattt aacacttgtt tacaaaactc atataagttt tctacttttt
     3421 cattgtcttt cgttcgccaa ctaaataaaa acttagggtg caagtctttc ggtaaggcat
     3481 ttgcaaaata ccgagtggtt tgttcattgg aaaccacaaa caactgtaag gtcgaaaaga
     3541 tattgttccg aaacatacct tcttctgcat attttttgat ttggttaaat gcctgataaa
     3601 caccatcttt tgcagttact tgtttcagct cgatttggac aatcggtaat ccattgatta
     3661 ataaggtgac gtcaaaacgt cgatcccgtg cttctatgtt acttccacgt tttgcaattt
     3721 gatggaccac ttcataagta gagattcctc caccaatgtc ttgattggaa taaaggataa
     3781 gtgagacaga acccaattgc gaatcttccc gttcaatcgt gatccgagcc atcccatttt
     3841 caccttttaa ccatttcgca gcatcaaatg gggttttcgt tcgcagcagt aattccgttt
     3901 tgatcgtttc aaattcttta tcggtcaaag gaaaatcatt cagttctgct tgattatttg
     3961 agataatttt ttggcgtaaa ttgacccaaa ggtcttcttc tgattttaga tccggacgat
     4021 acgtccactg attatgccct tctccaagta cacgaatcaa ctgttcttcg acttctgctt
     4081 cctcacggtg actaattttc attcttcttc cctcctagac aaacatcttt tgtaagaagc
     4141 tttttttcgt ttcttttagt aaatctaact tacgttgatg aagagcgatc gtatcatcta
     4201 gctgtttgaa gaaagtaccg attttttgtt gttcatcctt tacaggaacc ttaacactga
     4261 tattctcaat atcagaactg ttataagaag gcaatgcccc tacttgggag tatcttggca
     4321 aatttatagt ttcaaacaat attttaccaa aatagatatc ccaagtatta ttaaataaat
     4381 aagccatggt attgttatct attaagaaag ggcttgtgac caaacgtttt ctattaagca
     4441 ttattgctgc gccaacttta gcaaatataa tcgctggcac atcttttatt ggcttccatt
     4501 ttttacgctc aatttgctca ttagatacat aatgatttgc atttctcatt tcatactcat
     4561 taccaatgtt attcatgtcg gaaactttaa aaaatggagt accttcagaa ccaccttgct
     4621 cggcatcagg aaatccaatt cccgattgag ttttacctaa ttctcccaac ttacgctctt
     4681 cccaatcttc cgtaaaacca ggaaaccgga tttctggcac ttttgctccg ttttcaggga
     4741 acattttttg taagaatcct tttttcgttt ccttcagcaa atctaactta cgttgatgaa
     4801 gagcgatagt gtcgtcgagt tttttgaaga atatgccgat tttttgttgt tcttttaaat
     4861 cattgggaat atagaatact gtatctgaaa cttttttcca gtctgaacga ggcatttttg
     4921 ttccagatgt atcatttgca gccttttgat atctatcagc ttgaataaga gaataaataa
     4981 agtccgagtc gctcttctta gctctaaaaa cccagaaatc cccaatcgct acgcctgtaa
     5041 aagttgcctt gagccaattt tttaaatatg gacgtaactt tccatataga atatcatttg
     5101 gtaaaaattc aatccctttt ctattatcaa atttctggct cacatcttta ttcagtctac
     5161 cttctcctgc cacgatatct tcaaattcaa cttttggaag tctactggag tttgtacttt
     5221 tattaagtct aacaactaaa tcaattaact tacgctcttc ccaatcttcc gtaaatcctg
     5281 ggaaacggat ttctggtcgt gtatcattgc tcatgtttga acacctccag cgcaccttgt
     5341 aaccacattt cgttttcagg tgttgtttgc agggaagaga tagcttctaa aagttctttt
     5401 tctagcactt gctgttcttg tcgtatcttc ttgatgtcat ttccgacatg aaccatatca
     5461 attggttctt cttcttcgaa agtatctaca tagcgtggga tatttaagtt atagtcattt
     5521 tctttgattt catcaaaggt tgccaaatga gcgtatttct cgacatcttt tctttcagcg
     5581 taggtttcga ggattttttg aatgttttct tccgatagtt tattttgatt tttcccttta
     5641 acaaattcac gactagcatc aatgaataat acatctcgag tttgacgatt cttcttcaac
     5701 acaatcaccg tcgttggaat cgatgtccca aagaacaagt tcgcaggcat accgatcaca
     5761 gcataaatac tgccatcttc tagtaatttt tgacggatga ccccttctgc agcgccacgg
     5821 aataaaactc cgtgtggtaa gacgatggcc atggtacctg tttccttcaa atgatagaac
     5881 ccatgaagaa gaaacgcaaa atctgctttg gattttggcg ctaatttccc ataacgatta
     5941 aaccgagaat catctaagaa agtcgtatct gctgaccaat tagccgaata aggtgggttc
     6001 attactactg catcaaatgt gtaaggttca tctgttggcc agtccttatt caacgtatcc
     6061 ccattgcgga gattcatttc ttcggcgtcg acaccatgta aaatcaagtt catttttgct
     6121 agattgtatg tcgttgtatt tagttcttgc ccatgatatt taacgttatc tgggtgtgtt
     6181 aagtagttac gtacgtttaa catcaatgaa cctgaaccca tcgttggatc aaaaacacta
     6241 aagaatcgtc gttccttttg atcgagcgtt acgatttgcg ccatcatatc agaaaccata
     6301 tgaggtgtgt aaaattctcc ggctttcttc cctgcttctg aagcaaattg actgatcaga
     6361 aactcatacg catccccgat cacgtcccca tcgtgttcta aaacatctac atcattcaat
     6421 ttcttgatga cttcggtaat cgtcacattt cgttgttgct catctgttcc tagttttttt
     6481 gattggagat ccacatcatc aaataaccca ttaaattgat tgtaggtaga tgccaattgc
     6541 acaaaggctt tgtttagatc gttcaattgg aacgttgctt gttttgcttg atccgctaag
     6601 acgttgaaaa gatactctgg tgcaattgca tatcccaaaa tatcaacgat tgtggcaatc
     6661 agatcttctt ttgattcttc atctgaaagt aattcacgat acaacattgt ttgtttgctt
     6721 actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaatcga
     6781 tcggataaat atttatagaa aatcagtccc aataaataat ttttatattc tgacgcatcc
     6841 attttactac ggagattatc tgccgcacta aataattt
//
LOCUS       DACCSK010000127         6733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCSK010000127 DACCSK010000000
VERSION     DACCSK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.130"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(165..440)
                     /locus_tag="IVP39_002376"
     CDS             complement(165..440)
                     /locus_tag="IVP39_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587432.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            894..1100
                     /locus_tag="IVP39_002377"
     CDS             894..1100
                     /locus_tag="IVP39_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587433.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1296..1463
                     /locus_tag="IVP39_002378"
     CDS             1296..1463
                     /locus_tag="IVP39_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7587434.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1489..1833
                     /locus_tag="IVP39_002379"
     CDS             1489..1833
                     /locus_tag="IVP39_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7587435.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1838..2119
                     /locus_tag="IVP39_002380"
     CDS             1838..2119
                     /locus_tag="IVP39_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587436.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2222..2536
                     /locus_tag="IVP39_002381"
     CDS             2222..2536
                     /locus_tag="IVP39_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7587437.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2514..4208
                     /locus_tag="IVP39_002382"
     CDS             2514..4208
                     /locus_tag="IVP39_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7587438.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4228..5406
                     /locus_tag="IVP39_002383"
     CDS             4228..5406
                     /locus_tag="IVP39_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7587439.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5369..6055
                     /locus_tag="IVP39_002384"
     CDS             5369..6055
                     /locus_tag="IVP39_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7587440.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSAGTEAKKEQSVEEILNDVEEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            6055..>6733
                     /locus_tag="IVP39_002385"
     CDS             6055..>6733
                     /locus_tag="IVP39_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7587441.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVNNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKTAE"
BASE COUNT         2406 a          964 c         1398 g         1965 t
ORIGIN      
        1 ttggagggaa gaaaaatgaa attatcagta gaaggcactc cagaagaaat acaagaattg
       61 ctccaagcta ttggtagtag caaggagcaa aaggtaagtg tttctaattc agatattgac
      121 ggtatttgca attgtttaaa cagagaaaaa atatctaatc cttactaaga gaaaatatgt
      181 tcgttaatga atttccaatt ttcctctgta gctctacctt gataattttt ttgcagttca
      241 cagatgaata aggaatcgtt attatcaagt actgtattta atttttcaca catttgattt
      301 ggattcaaat tagttttaac taaccaaaaa gatttttgta gctttatata tgccccaaat
      361 tcttttatta tttcaaatac tttatcatac ttttgccctg ggttattaag atcataacta
      421 atcatgtagg gtttatacat gttgtcttgc acatccttgt tttatatttc agcggaccac
      481 tcgctgataa ataaaattat acgcctagta tttattttca caatattaat ttatcgtaat
      541 aacttttgag taaatagaac aaaaaacctg cactagtttc cgctagtaca ggcagtgact
      601 atatcggttg ataatctagc atatattgaa aataattgca agaaaagaaa tttatagtgg
      661 tttataccaa attatcaaag caatgttatt ttgttgctgt ctattgtttt ttaattattc
      721 acacgataac taaaggtggg tgaagagaaa tgattccatt aataatttca atttttgcgc
      781 tctgtcttaa tgtctatatg attggattta aaaatgggca aaataaaaaa tagtagcagc
      841 caagaataat tttatagtgt cactgtggcg gaaagggtag acgctaagca tgtgtgctag
      901 gtcaatgctt cggcaaccat gcaaggttcg attccttgcc agcgacatta aatgcctatg
      961 acggttacga ctaccgaaaa aagatcgtta agaagctata cggtgctacg tacggcaatg
     1021 tagtaagtgt gctatctgta caccaccaag cttcggtcac tgtggcggaa gtagaagacg
     1081 cagcggtaaa tggcgagtag cctcgtgaga gcctggtaag ttctcgtgag tggtgcaatc
     1141 ccactccagc gactttaagc aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa
     1201 tttcgtacgc gttttgtgca tcgtgcaagt tactattaca tattagatca ctctttgagt
     1261 ggtcttttta tttttgcaca aaggaggaaa caacaatgta tagaccacaa tacttagaac
     1321 agaagtatga agtaatcact gttcataaag gtaatggcga aaaagtatat gagtatagaa
     1381 gaccaataaa gagcgataca tataaacgaa aggaaaacaa tgaagttatt ccattgtatg
     1441 gcaaaagaat agctaagcat taaataagat tgcgaaagga gacggaacat gaccgaggaa
     1501 ttctatagat ggctattaca gttgacaaga gaagatcgtt tggttaagtt ctatcagtct
     1561 cctaaatggc gcaggcttag agagaaagcg atgaaacgag atcactatga atgccaagag
     1621 tgtagaagac taggtaagta tcatagagta gagaacgttc atcatataaa ggaagtcaag
     1681 gatagacctg acttagcttt agatttagat aatcttattt gtttatgtgt tgaacatcat
     1741 aatgaagttc atggcagata tcttacagca ttagataaac aagagaagaa gatagaaagc
     1801 tttgctaact tcgatgcaag tgaaaggtgg taagtgcatg atcatcaatg ataatggcag
     1861 agagtatgat acagaaaaga ttgaagagta ttcatcttat actcagggat taattaaacg
     1921 tttgatatac gttcgctatg taggtattag ggatctgtta tcagataact gttgtagtaa
     1981 atacaaagtg aatcaagtaa gagaagcgtt gaataaagat aataacgtcg aaagaataaa
     2041 aaatgttttt ggatatagta ttgaagagat taattattac attgacttcg ctgaagcttt
     2101 cattccgatg gtgagataac ccccccttaa aataaatcgc aaattttttg ggggtgatga
     2161 aacggagggg gctgtcagga aaagagattt tttcgaactt tatcatgaaa ggagggctaa
     2221 aatgtttaaa aacgaattgt ctcaaaatcg ctacagagaa aaattacgcc gctctttaat
     2281 aagccaattg gaaagtcaga aaacaaatat tgagccattc ttagataatg ttgatcgtta
     2341 tatcagttta tgggaaacgg cgatatcact ggaagaagat atatccgaga acggcattag
     2401 actggagaat ggtaaaaaga atgaatcagt agcgttgctt gtttctgtca acaaacaaat
     2461 gggattgatg ttggataaac ttgccattac tcctgaattg gtaggtgaag caaatgaatc
     2521 aattcctgag ttataagcat attgaaaatt ggttcaaagc tatagaagaa ggcactatca
     2581 aggtatgcaa agagcaatta ttgctaaaaa attatctaga agaaagagtc tttactagag
     2641 aagatattta cttcgataag cagatggtag aggattcaat caatatacca gcacaatact
     2701 ttccattcga attaattccg tgggaaaaat ttctacaatg ttttatttat ggtgttcgat
     2761 ggaaaaaaga taaaacacta gtgttcaata gatatctttc attaatggga cgtggtaatg
     2821 gtaaaactgg ttttgcttct tggaacaact tctttctact aaccgctaaa cacggtatta
     2881 aaaattatga tattgatatc tatgccaata atgaaagcca agcaaagact agttttgatg
     2941 atgtatttaa agtaattaaa gatcatcctg atttagataa aaaagtattt aaagctacga
     3001 aggaagttat tcaaaatatc gctacaaaca gcaaacttcg ttataacacg gcaaatgcta
     3061 gaacaaaaga tgggaagcga ccaggtgcaa accgctttga tgaaattcac gaaaatgaag
     3121 attattcaat gataaatgtg gctacttctg gtggtggtaa aattcgagat tatagagaat
     3181 tttatgatac aactaatggt catgttcgtg gtggtccgct tgatgacatt atagaagaat
     3241 caaaaatgat tctttctgga gaacttggaa ttgacaagga tggagcagaa ttttctagtt
     3301 tgtttccatt tatttgtcgc ttggataacg ataatgaagt tgatgatccc gacatgtggg
     3361 aaaaagcttg tccaactatt aattacaatg cagatttaaa acggaaaatg tttcaagaat
     3421 actctcaaat gcaacgtaat gctggtttaa gacttacgtt catgaccaaa cgaatgaaca
     3481 gacctatgga agatacacga tttgctgttg cttcatatga tgatgttctg catacgaaag
     3541 aaaaagaatt tcctgaaaaa atggatgaag tgataggaac agtcgatttt gctgatagac
     3601 gagattttgc cagcgttggg ttgctaggaa aatacgataa agatgtttat tttacacaac
     3661 atacttttat ccacgaatca gcccttcgat tacaaaacat caaacgagag gttatagata
     3721 tttctataga tcaaggaaaa tcacagatcg ttcatggaaa aaatatagaa gctgattata
     3781 ttgtaggttg gtttcttgaa atgagtaata aatattatat taaaaaaatc gctatggata
     3841 tgtaccgtgc aaaaatattg aagcccgctt tagaagaagc aggttttact gtggaaattg
     3901 ttcgaagcgg atctgttaca catggtatgt taaaagatct ggttgatgac ctttttatta
     3961 atcaacgttt attttttggt gacgatgcga ttatgcgttg gtattgcatg aatgtatatg
     4021 aagagcatat ttctaatgga aatatacgct atgaaaaaat agaacctgaa actagaaaaa
     4081 cggatggctt tttttcattc cttcatggtt tgaatttttt agatgatatt tatgattctg
     4141 ctcctgtaac agtcacaaat agctcagtag aaaatacagg aactggattt actcctctag
     4201 tattctaact tgaaaggagg tgagaaagtg gggatttttc aaaaggcggt aggatacttc
     4261 acaaaaaaag caacggttcc tttagaagaa tacttttgta aattgcaagt tgattttgtg
     4321 tatcgaaaat ttgcgattga aacttgtatt gatttgattg caaatgcgat gagtaaagcg
     4381 gaattcaagt catatgaaga tggaaaaaat aaaaagaatg atctttacta taggctgaat
     4441 gtagctccta ataagaaaaa taatgcaaca gaatttagaa aaaaactgat caggagatta
     4501 atattctaca atgaagtatt gatcgtttct ccgtctaata attctagcga aatatttatt
     4561 gcggatagtt gggatgtcac agaatatgca ttgaaagatg atgtgttttc tcaagtgcaa
     4621 attaacaaca tagtccttga tagagaattt ctagaaagtg atgttatcta tataaaatac
     4681 gcagatcaac aaattaggca actagtcgat gcgtattatc aagcgtatgg gaaactcatt
     4741 tctagtgcca tgaatgttta caagcgctct aacgctcgta gatacgtact gaaagggaat
     4801 ttattccgac cgcaagacaa tacaacacaa gatcaaatca ataaaatgat gacatcacaa
     4861 tttaaggctt ttatggaagc tgataatgca ggtgcggtat ttcaattaca aaatgagtac
     4921 acattagaag atttcagcgg aaacttccaa agcaattcaa gagatataaa aaacttaata
     4981 gacgacatct ttgagatgac agcagcagcg tttcacgttc cgaaaaacct actaaaggga
     5041 gacatgagtg ggttatcgga tcaagtggac gcttttttaa tgttcgaaat cataccgatt
     5101 gctgaactta ttcaggatgc gtttaacgct agtctctatg aagtagaaga atacttgtca
     5161 gggaattttg tacgtgtgga tacaactatg atcaagatta ctagcttcaa agatttggtt
     5221 gacgctattg atgtaggcat tagaaatgga gtatttacaa tcaacgaagg aagagaacgc
     5281 gttggaaatg atcgctctga taaggcgatg gcagatgaaa tatttataac taaaaacaat
     5341 caacaagtat cgaaaggagg tgaggcgaat gacgacaatg aaaacatttc tagcagtaaa
     5401 gaatgaaggc gcagtaccgc aaatttttat tcagggattt attggttcta gttggttctt
     5461 tgaagggaat actgacaagg gaatcaaaaa tattttggat agtctaggtg atcaagaaga
     5521 aattgaagta gtaattaatt caaacggtgg agacgtattt caagggattg ctattgggaa
     5581 cttacttaag tcaaataaag caaaagttaa cgttgtgatt aacggcttag ccgctagtgc
     5641 tgcttcaatt atcgcaatgg ctggcgatac tataaaaatc tacaacaatg cacaattgat
     5701 gattcaccgc gcttccacat acggagaagg aaatgtcgat gacttccgta cgattgctga
     5761 ccaactggaa tcaattgata aatcggtaaa ggcttcatat aaaacacgat tcaatggcac
     5821 agatgaagca ttgcaagaac ttcttgaaaa agaatcgttt atggatgcag aaacagcttt
     5881 gagttatgga ttggtcgatg aaattatcga tgcagaaaat agcgcaggta ccgaagctaa
     5941 gaaagaacaa agcgtcgaag aaattttgaa tgacgttgaa gaaaaaagag cagaaaaaat
     6001 tgctgcattt acagcagcat taaataaaac atttggacaa ggagatgcaa aataatgaca
     6061 gttaaaaatt taaaaggtgt aacagctgca agcgaccaat tgatgaaagc ttttaaagat
     6121 ggtaacgaag agtcttttag cgcagctatg gtaagtttat ctaaggaaat acaggataaa
     6181 attttagaag aagcaacagc aaaaaatcaa gatcaattag tattaatgaa ccgtgggcag
     6241 cgtgtactaa ctacacaaga aacaaaattc tataacgaag tagtgaataa tgaaggtttt
     6301 gcaggagttg aagaattagt accagctact gtatttgaac gcgtatttga agatctagaa
     6361 caatctcatc cactattgca aaaaattact tttgttaaca caactggtgt aacagaatgg
     6421 atcgtgtcac gtggagtcaa tccagcatgg tggggcaaac tttgcgaagc tgttaaaaaa
     6481 gttttagata atggctttga cgtaattaac atgaagcagt tcaagctatc aggttatatt
     6541 cctgtatgta aggcaatgct tgacttaggt ccagtatggt tagatcgtta tgttcgtact
     6601 gttttagttg aatcattgag aattgcatta gaacaagcaa ttgttgatgg tactggtaaa
     6661 gatatgccag tcggaatgat gcgcgacatg agcaaacaaa ctagcggaga atatgctgaa
     6721 aaaacagcag aac
//
LOCUS       DACCSK010000128         6697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCSK010000128 DACCSK010000000
VERSION     DACCSK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.131"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            52..789
                     /locus_tag="IVP39_002386"
     CDS             52..789
                     /locus_tag="IVP39_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7587442.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            817..1356
                     /locus_tag="IVP39_002387"
     CDS             817..1356
                     /locus_tag="IVP39_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7587443.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1856..2317)
                     /locus_tag="IVP39_002388"
     CDS             complement(1856..2317)
                     /locus_tag="IVP39_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587444.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            2450..2824
                     /locus_tag="IVP39_002389"
     CDS             2450..2824
                     /locus_tag="IVP39_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAP7587445.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            2878..5004
                     /locus_tag="IVP39_002390"
     CDS             2878..5004
                     /locus_tag="IVP39_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587446.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLESIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            5001..5621
                     /locus_tag="IVP39_002391"
     CDS             5001..5621
                     /locus_tag="IVP39_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587447.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            complement(5779..6687)
                     /locus_tag="IVP39_002392"
     CDS             complement(5779..6687)
                     /locus_tag="IVP39_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP7587448.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         2594 a          946 c          904 g         2253 t
ORIGIN      
        1 ttttacatcc tcttatgtct atgataacat aatttattga ggtgattttt attgagtaac
       61 gatatagttc gatataataa tggattaaat tctgtccctt taagaaaatt tacccctata
      121 gatatggatt tattttgggg aatatgttct aaaatgaaaa gaaaaggtac aaaagaagaa
      181 atttttacat ttaaacaaat aaaagaatta attgattaca atataaatgc acgtggcgac
      241 cgttttgcta atgatttaat aaaaatgtca aaaaaaatca ttactttgtc ttttatttat
      301 gaagacgaaa aaacatatga acaattaaat ctgtttcaaa aatttaaaat cgataaagtg
      361 aatgaaacat tattaataaa ggtttctgaa gaatttgaat atatacttaa ttcaatcgga
      421 actaatttta ctcgtttcga attagaaaat atgacaaact taaagagtag ctacgttaaa
      481 gagttatatc gccaacttat ggctcataaa gacagaagat ctaaaaaagg tcactggttt
      541 gtaaaaatag aagattttag agctctatta gcaataccag aaagttatcg catgagagat
      601 attgataaac aaatttttgc ccaagcgaaa aaagaatttt taaacgttaa ttcaactcac
      661 cctgctattt ttaaaacttt tgaaatcaaa aaaattaaag cctacaaagg aaataagata
      721 ggatctctgc aaatttattt tgaagaaaat ataccttcaa ttaccttaga taattggtta
      781 gagaaataat acctaacaat ttaggaggca attatcatgc aacccaaaga aacctttaca
      841 ataaaggaat tatctgaatt atttgaaata agtaggcaag caatctctaa acatgttgat
      901 aaattagacc cgtccttatt agctaaaaat gagcgtggtt ataaggttgt attacgttct
      961 ggagtattgc aacttgctag caacttaggt aaccaaaggt tactatatag gttgcatgag
     1021 tatgaacaag aaaacgataa aaacgtttat atatcaagca aaaacgtgat tgtaaacgag
     1081 ttgctagata agttgcgtga agaaaatgaa tttttacgag aacaactttc cctcaaagag
     1141 caaaataatt ctaatatgca aaaattactc gaccagcaac aacagctcac cctacaggca
     1201 aatcaacaga tcgaaaaact gcaagaacag ttacaattag cttatacgga agaaactcca
     1261 gaagatgaat caactactat tctttctgaa gaaacagaat cgatagaaaa acacacaaaa
     1321 aagaagaagc aaaaatggtg gcaactttgg aaataactgg agggaacatt catgacaaga
     1381 aaaaatcttc tattcggttc tattttacta ggaattctag gattcagtcc gatgttattg
     1441 tcagtaatca ataaagggaa taaacatggt gaattgaaat tgaatccaaa ttattacaac
     1501 agtcctaacg aagaattgtt tgtaaaagag acggataaat aataaaaaag actgctcttc
     1561 cgagcagcca gttaaaaaaa taatgagagt ttattcttga aaaagcaacg cgcttataaa
     1621 gcgctaccaa aattttatca tattttataa aaaataacaa tccagtaaaa aatgactggt
     1681 aaaaattcac aaagaaacgg aaagaagcaa ctacagaaaa tcaagcctta aaaagagctt
     1741 tttacctaga ccaattaaaa atcacaaagt gagcgtctct atccattggt agaggggcaa
     1801 cttggtctag tgtacattat gtcaactatg caattaaata actaactact tcatattagc
     1861 ttaataaaat aatcaaaatc actaggggaa aaaatattgt ataaaaaaat ttttcatttt
     1921 ttaaataaga atagtaaata taaaaataat ttaagatatc tattactatt aatccagata
     1981 gagcaaacca agtaaatatt ccgtgtgtaa agctaaaaaa attcatttga atcaattcaa
     2041 ataaacctaa tatagtaaca aatatattaa aaataatagc tgtcaatacc agtaaattat
     2101 tttgtttatt ttttaaatta tcttcattta aaatagaatt tatgttagta ctataaaatt
     2161 cacaaatttt ttttagtgtc tctatatctg gtgttgtttt gttattttcc cactttgaaa
     2221 tagtttgacg tgtaacatta aattttcttg ccacatattc ttgagtatac ccttttttca
     2281 ctcttaattc tcttagctta gatcctaaat cctccatttt ttctcctatg taaacgatta
     2341 tttacataaa attttatcat aaaaatacta acgcgccaaa aaagttcttt attttataaa
     2401 acacactaaa ataaaataat tctagtaaaa aagataggag aaataagtta tgaaaaaatt
     2461 tttatgtctt tctgctttaa tgggtgtctt attaactagt ggaggaatag ttagtgctac
     2521 agaagcttta aatctagatg tacccgaaga ccataccgcc atttacggag gaggaatgga
     2581 agctacagaa gaaggatttt cttctagtaa attgagatat gctgctggcg gcggagattt
     2641 caactgtggg gttaatggat ttaaagttta tgcaaattac taccatgcta gagctaaaca
     2701 tagtgcaaca gctaaaaatg gacgaggcgg tcaagtacga tcagttcaga aagctggagt
     2761 aagagcttat gcaacatgca atgccacact tactggaaat actggttggt ggaatgttta
     2821 ttaaaaaaga gagtttaaac tctctttttt atttacattt gaaaggaaat tcaaaatatg
     2881 ttaaaaaaaa taaacgctgt tttatcactt tttgtctgca ttacaatact attcttttct
     2941 actaatgtta taaatggtat aaagacttct gaagcgctat ataaaaatac cgctgaaatt
     3001 catatgcaat ataaagatga tatggacttt aacagttata taaacgcttt gtatgaaata
     3061 tctacacaag aaaatattaa tatttctcaa tatagtttta ctgctgttaa ccaactaact
     3121 attcttgcta caaaccctaa cgctaacaaa cactggaaat tttctaaaaa aaatatatta
     3181 gcaagaaacg attctaaagt ccattatact aataacaaaa attctaagta ccaattaaaa
     3241 cttccaaata attttttaaa tataaatatt tatccttttt ctcatgtgga aaatattgga
     3301 ttaagtgaaa tattatatgt acaaggaaac tcaaacaaat taattcctgt actgaaaaaa
     3361 tatggaacac ctactatttc caatatcaat caatcagata cttttcaaat taatactaat
     3421 attgttcttg ttatctgcta tcttagtata tttattgtaa ttactacaat tatatttgct
     3481 ttttcaaaaa tgaaagaaat cactctaaaa aaaatgttag gctataactc ttttgatact
     3541 ataaaagatt ctattaaaga gttaaacctc ttattttgtt ctttgattat aactttcatt
     3601 ttatctattt ttttgagcat atattacagt aacgaaaatt ttttcctatc atttcttcta
     3661 tgtagtattt ttcattttat aattagtttt ttatttatca tagttactct aagtatctat
     3721 catttatcta gtataaaaaa ttatctaaaa aattcacgac cacttaagtt aaatctttgt
     3781 atattaaata tctgtctatt cttaagcatg atccttctgt taatagcttc tacaaaagta
     3841 ataaatatcc ataatgaagc cgaaaataat tctcttaaat attgggaaag aactactaat
     3901 ttatataaaa caaatattac taatcaatta aatagaaaca acaccgtaga agaaaataac
     3961 tacttgaaaa aagctagtaa atttgtttat aaaatacaga agaattataa aacttttatt
     4021 atcgcgccat acaattacgc aactatccaa gaaaataata aagaattttt tattggacaa
     4081 aaagtttatc caaattttga agactatgtt agtcaacctg ctggtgcagg aatatgtgtt
     4141 gatttaaact atctaaaata ttacaatcct atttcttttg aagaatctac tgacttaaat
     4201 ttaataaact ctgatccact aactacttac attctagtac ctaaaaaata taaagaatat
     4261 gcaaaaatga ttgaaaaaaa ttatttagaa gacatacagt ttagacttga agactctcct
     4321 cctcaagctc cttataaaac tcctaatttg aaaaatttaa aaataaaact aatatttgtt
     4381 aataataacc aaaagtattt tagttttaat accttatatg gaaaggcaaa agataactat
     4441 acaataactg atcccatagt tagagttatt aatccagaaa taacagaatc attattttgg
     4501 ggaaatatat tgactagtga tggtggatta ttttttgatc agaaacatac ttcaaaccaa
     4561 aactttttta atcaaattaa tccgtatata aaagaattta acctagaaag tatcataaat
     4621 ttttcttatt ctgtttttca agaaactggt gaattattat ctcaaaaaaa agtaaataaa
     4681 ttaaatgcat atctacaata tcttttaata ttaggattat ttataataat taattttcaa
     4741 atgatttttg ttagttttac tataaattca aaaaaaatag ctttaaaaaa aatatttgga
     4801 tattcaccaa taagtcaagt tttagattta tgtttactcc cttttacaat agagttattt
     4861 gctactatgt gttttcaatt gatattttca aaaaacactt acttgtattt tataattttt
     4921 atgatctttt taataaatct aattacttct attattattt ataaaaaaaa tagttttaaa
     4981 tattttaagg agaatatttt atgatattat cagttgagaa tttcaccctg tcaattaata
     5041 aaaaaaagat caaacaaaat tggaactttt caatatcaga aaaagatttt attatagtaa
     5101 caggaaaaag tggtagtgga aaaactactc tgttaaataa tttaagccta atagataaac
     5161 aatataccgg taacttattc tattttgaaa agaaagtttc taaacatgat atacaacgtc
     5221 tacgaagaaa tcatattagc tatttatttc aaaactatgc tcttcttgaa aatcaaacag
     5281 tacaatacaa tttcgaatta gcaaaaaagt ttaacagtaa tatttccaat gatcatattt
     5341 ataatttatt aacagatttt tctttgccaa aagatatctt aaaagaaaaa atctttctct
     5401 taagcggtgg agaacaacaa agaatagcat taattagaaa tatgttaaaa ccatttgata
     5461 tattatttgc agatgaacct acaggaaatt tagataaaga aaactcaact tttataatga
     5521 actttctgaa aaatttaaca gaaaaagaaa aaaaagccgt tgtactagta acacatgact
     5581 taaatgttct aaactatgga actaatatta tagaattata atttttaccc gtgatgctag
     5641 ctgatttaaa actagcacca cgggtattta ttttatattc catctctcct atcccaaaat
     5701 ttaaataatc acttaataaa aaggaggcac ccaagtgatt ttattgaaaa aagagtatac
     5761 ccgtggtgct agttaatttt aatatcctaa agaatatctc aaagttcctt caaaacgtcg
     5821 ttgtgctcga cttaatagaa cactgtaagc cagtaatatg ctttcaaatc tactctctag
     5881 ccctttaaca gaacgtgagt tgaaattttg acaacctaat ttttctaagg aagaaaaaac
     5941 agtctcaact gtttttcttt gttttccaag taagctgtgg tctagttttt ctgatttatc
     6001 catgttttta cgtggcggaa cggatatagt aatgccctta agggcaagct tttcatggat
     6061 tttatggcta atatatcctt tgtcccctag gatattggga agattcgctt cttccgataa
     6121 agtctctaac actttcacgt catggacacc tggattcgtg attgagtaag taatcggaaa
     6181 gccagtctta gtaacgatca tatgaatttt aagaccataa aaataagatt tttttgttga
     6241 gttatagcca actttggcta tttgattcaa tagttttgct tgtctatttc taactggttt
     6301 acataatgga ctaggaaaac tatctataat tccgactagt tcacctttgg tgagcttctt
     6361 gatgaaaaag tatcgaataa tcttgatagt gtaagctaag tttgaactta agcgagtgaa
     6421 tcgactccta ctaggaaagt caccattagg aaataagacg gaacaaaccg ctctatacgt
     6481 ggctctttgg ctagtatagc cattaattat gccccatata acacaagcaa taatcactgt
     6541 atcgcgttgc ttcagttgat cagtatttct tcggttttta atcttatctg gtacacaatt
     6601 gtggtatatg ttagaaacag ttctcataat ttccttgaat gttataaaag tatctgtata
     6661 atgttcagta tattttgatg aacgcatatg tagtcct
//
LOCUS       DACCSK010000129         6671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCSK010000129 DACCSK010000000
VERSION     DACCSK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.132"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(93..2192)
                     /locus_tag="IVP39_002393"
     CDS             complement(93..2192)
                     /locus_tag="IVP39_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7587449.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(2412..3521)
                     /gene="rpoD"
                     /locus_tag="IVP39_002394"
     CDS             complement(2412..3521)
                     /gene="rpoD"
                     /locus_tag="IVP39_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7587450.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(3545..5386)
                     /locus_tag="IVP39_002395"
     CDS             complement(3545..5386)
                     /locus_tag="IVP39_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7587451.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(5568..>6671)
                     /locus_tag="IVP39_002396"
     CDS             complement(5568..>6671)
                     /locus_tag="IVP39_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7587452.1"
                     /translation="MLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEE
                     GVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQ
                     QKKLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDF
                     TTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLA
                     IEGYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAET
                     TTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGES
                     VGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
BASE COUNT         1652 a         1431 c         1164 g         2424 t
ORIGIN      
        1 aaaaggagta tgacaacagt tgtgtcatac tccccttttt cgaataaacg gtgttaaacg
       61 gcaggctcac agcctctttc atttgcctaa aattaattgc tcccatttcc tccgaaccat
      121 ccagttttac agttgatggt tacgatgtaa aatggcaaag cactttctcc aatgcctttc
      181 atcaatgcag attttgttga agtggcatat agattataat agccttcccc attttcgatt
      241 ctggcttttt ctaatctgta acctccttca acgatatatc cacgttggat acatgtagca
      301 atcactttgc tttggacacc tggtgcccac gtccatgctg gattagatgc atcggcaata
      361 tcacttgctc tggcagattt gattggctga ttcgctgagt ttgaagagga aggggtcgag
      421 gatgccgctt cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc ttgagctgca
      481 gctgctgctt gagcggcttg ttttgcttgt tcttcagctg cttttttcgc ttcttctgct
      541 tttttctttt cctcttcttc ttttttctct ttatcagtga gcatcttatc tacttgtttc
      601 aattgttcat tcaactgagc agatagatct gtatttttca ctttttctac tgcttctttt
      661 gccgtcttgt attggtcgcg tgttgctgaa tccactactt ttccgtcttt aaagactata
      721 gctgtttttt ctttagctgt ttgcaattca gcatattgat ttttagcttc tgtttgtgct
      781 tgttgaatca attttccaaa atctgtgtta tccgtttcga ttgcttggat ctctttatct
      841 gctttcaact tgatgtcatc tgctagatga tcatcgacga tggcaggtgc tatgaaccac
      901 tcattcactt tttggatatc tcggatctta gattggatat cttggtaagt tgtttctaac
      961 tctgaatatc ctttttcatt cttaagactg gataaatcat tttttagttt tgataaatct
     1021 tgatgaatca tatctgcacg aataaattga tggtgatcat ctgtataaaa ggcagcaagt
     1081 ttctcatgaa gttcttccat ttgttgctgt ctttttgctt ctgctgctac ttcggcttct
     1141 actttttgat gatggtcata ataagcccag cttcctccac cgagtagaag tactgctgct
     1201 aaaacgagat aaggtacttt ttttgatttt ttcttttgcg ctttatctgg atctgttttt
     1261 tctaagtttg attcttgatg tgttttttca gtatctggca cgattgttat tgatttcgct
     1321 tctgcttcaa acagcttagg ctggttctct tctttttcta tttttgcttc aagatcagta
     1381 ccctctttgc ttacagattg attttcagca tcggattcac tatttatttc atcgggctct
     1441 gttgactttt tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg cagtttattc
     1501 gttttttcct tttctaattg tttttcagta aatacttcag ttttttctgt gatatccgtt
     1561 tttacgtctt ttatttttac tgtttcttta cctgtggcgt tttctctatc agagtctttt
     1621 tcagtttctt gttccttttc tggctctact ttgttttcct gtgcaatttt ttcactgatc
     1681 attttgagca gctctttcgc gtgctcttct tttgtatctt cacgatgtgc tttgatataa
     1741 gcggatagta tcgaattttc ttcctcatta tcgacgattt ctttcagttc agctactctt
     1801 cttttttgtt ttgattcaag cgtttcttct tgattctttc ctgattcttc cgaagtctca
     1861 tgaacagttg ctttcttctg ttcatttttc tctatattag ttacttgatc gtgagcaggt
     1921 ggttcttcgt cttctggttc tgctttttcg aataattcca ttacagattc tagaggtaaa
     1981 tctttcaatt ccgaccattc gattggatcg tttaattctg tatcaggaaa gactgcatct
     2041 tcagttgatt tatcctgcat agctgcatct gtatttgttt cttcaattga ttcttttatt
     2101 tgctgatctt ctgcagttat ttgatgaccg cagtctggac agatagtatt ctgctccatt
     2161 tttttcgatc cgcaatttgg acattttttc atcacttagc gatcggctcc tttactaggt
     2221 ttacgctacc atttaatcta tcatattcta tacgtcacgc caatagctta tttttaggat
     2281 ttacttaaaa ataatgaatg aatttccttc ctatagaaaa aaaagcttga gactgcgtag
     2341 tctcaagctt tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca
     2401 atagtccacg cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat
     2461 tttcttaatg ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc
     2521 cctacttctt ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca
     2581 ttctcttccc gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca
     2641 taagctgcgt gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga
     2701 gagtcatctt cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag
     2761 atttctcgga ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt
     2821 tctcttccaa ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca
     2881 accatatgaa ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct
     2941 tgacgaatcc accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt
     3001 tcaactgcct tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct
     3061 cgtcctacat aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc
     3121 tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca
     3181 gttaaaaggg agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt
     3241 acaccggttg gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc
     3301 ttcaagctgt gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca
     3361 atcttttgga tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga
     3421 ttggataaat catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct
     3481 actgctgctt catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga
     3541 gtagctattt ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa
     3601 gctcaagctg tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta
     3661 tttcatcagc taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg
     3721 aactttcttc tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat
     3781 cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat
     3841 cgaataaaaa ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta
     3901 aaatattacg gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt
     3961 ctacttgcgt caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt
     4021 gactttcctg aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt
     4081 tttgtatcgt ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt
     4141 gttcgattgg tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct
     4201 tgtctttttc attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga
     4261 ctgtttcgcg cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat
     4321 attcatcagg atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt
     4381 gccgaagcaa ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc
     4441 aaaagaccaa ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg
     4501 tccccataga agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa
     4561 acccttcgaa caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat
     4621 taaataaaac ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct
     4681 gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc
     4741 cttgagaatt acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat
     4801 catcatgcgt gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct
     4861 tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa
     4921 ttaattctaa agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg
     4981 tatgcaatag catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc
     5041 ccgtagcaga ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg
     5101 gtatctgttc gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt
     5161 cttgaagaaa agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct
     5221 tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt
     5281 aatttttacc agactttttc aactggacat attgtccaat cacctcaacg atattcgttt
     5341 gactgcgtac tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg
     5401 ctctattgat atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat
     5461 cgcactaact gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca
     5521 tatttcattg ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact
     5581 cttttcagta actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt
     5641 acgagcatga atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa
     5701 attccatgga acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag
     5761 atgcgcaatg ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc
     5821 agcaagtaat acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt
     5881 agatacaccg caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt
     5941 gctgtaggca aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag
     6001 gaaggatacc cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat
     6061 ccaattgctg taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa
     6121 aagatgcctg agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct
     6181 ggcaatgtag taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca
     6241 tcaccttctg ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca
     6301 ccgtcgcctg tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc
     6361 ttttgctgcg gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc
     6421 atttcttcag taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt
     6481 ctagcgatat tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct
     6541 tcctcatgaa gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca
     6601 aacagagcaa ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg
     6661 atccccaaca t
//
LOCUS       DACCSK010000130         6625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCSK010000130 DACCSK010000000
VERSION     DACCSK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.133"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(18..710)
                     /locus_tag="IVP39_002397"
     CDS             complement(18..710)
                     /locus_tag="IVP39_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7587453.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="IVP39_002398"
     CDS             705..971
                     /locus_tag="IVP39_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7587454.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="IVP39_002399"
     CDS             complement(1147..1716)
                     /locus_tag="IVP39_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7587455.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKELKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="IVP39_002400"
     CDS             complement(1790..3079)
                     /locus_tag="IVP39_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587456.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKVQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3433..4281
                     /locus_tag="IVP39_002401"
     CDS             3433..4281
                     /locus_tag="IVP39_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7587457.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="IVP39_002402"
     CDS             4426..5367
                     /locus_tag="IVP39_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7587458.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFELLLDLYFSLWHLRKEKQPE
                     LLILGSRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5722..>6625
                     /locus_tag="IVP39_002403"
     CDS             5722..>6625
                     /locus_tag="IVP39_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP7587459.1"
                     /translation="MTVDYDYWYAPFLSLMAYFMMLTVFRKKDLLYEMIAVVTVLFVY
                     LAARQFFSIRLLSGNTRFQSLTTVLLICFLTFSAILPLYLPKVHQSLEETSEPIREYL
                     NDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYWKIENKNH
                     YTGSGWIEDQDAPIQPIQEFPVDITSYQTVIEDKENITLTLKKQAFLPYPYGNTTLTD
                     FSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKADDLENQQT
                     NLQLPKSLPKRIEDLAVELTKNETT"
BASE COUNT         1941 a         1305 c         1196 g         2183 t
ORIGIN      
        1 aaagaggggg gaatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tcccttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttag
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttc
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttgt cagctttgat ttgttttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc tgtacttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgcaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatacaagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacttctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcagccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggtttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagtcacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaaac aactt
//
LOCUS       DACCSK010000131         6599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCSK010000131 DACCSK010000000
VERSION     DACCSK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.134"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            102..434
                     /locus_tag="IVP39_002404"
     CDS             102..434
                     /locus_tag="IVP39_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587460.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            435..1028
                     /locus_tag="IVP39_002405"
     CDS             435..1028
                     /locus_tag="IVP39_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587461.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            1050..2744
                     /locus_tag="IVP39_002406"
     CDS             1050..2744
                     /locus_tag="IVP39_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7587462.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            3432..5309
                     /gene="ltrA"
                     /locus_tag="IVP39_002407"
     CDS             3432..5309
                     /gene="ltrA"
                     /locus_tag="IVP39_002407"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7587463.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELTLSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     A"
     gene            5440..5817
                     /locus_tag="IVP39_002408"
                     /pseudo
     CDS             5440..5817
                     /locus_tag="IVP39_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            5817..>6599
                     /locus_tag="IVP39_002409"
     CDS             5817..>6599
                     /locus_tag="IVP39_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7587464.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNS"
BASE COUNT         2335 a         1073 c         1445 g         1746 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaaacgct gtgagaaatc acagtgtttt
       61 ttatttctaa aaatacaaaa tataaggggt atggttatat tatggataaa tacattcata
      121 tgaaaaatcc tccaagattt agtggggaat ggaagttttt cgcctttttc tttttagatc
      181 tattaacgct tggtggctgc tttttttggg cgaaaatagt caataatctt gtccacttac
      241 cactcttttt tgcactcttg aattatggat tatggcttgc gatagcgtta gtgattgttt
      301 ggcgacccta taataaccct ggtcaacgcc aaatttttgc gattatcgaa tctattttct
      361 ttacggataa gaaccactat catgcgttag atcctaaccg aaattatcat ggtacgtata
      421 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgataagaaa tccattttgc
      481 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
      541 aagagggagt cttaattact agagaagatc attttcagcg ttattatcgt gtcgtttcca
      601 ctgacgtgga gggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
      661 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
      721 cggaacaaat cgtgcaaaag cggcagttat tagataaaaa tcgtatggaa cagtcactag
      781 gaagaaatct acagcgatta agaaaatatg aaaagaaatt agtagaggag atccaggaat
      841 taaaacgggc agaacaagaa cgtcctgact taagcttctt tttcgttatt gaaggaaaga
      901 caatcaagga tttacaaact agaaatcgtc agttaatgcg atctagtggc attttagggt
      961 taaaaccact ctacaaaaaa gaattgatta agattcttta tcgaatgaac aacatgaatg
     1021 acgaataaaa aagatggagg aaatgattca tgctagatgt gataaaaaca gctttttcaa
     1081 cgcaaagtaa tcaaaaggtg attgatccat taaaacaact tggtaatcgt gaaatagaga
     1141 ttttaaaatc aaaaggttat gatttagatt tcttaaaaca agtgatgcca caaggtgggt
     1201 tgaattttga tgaagaatat gtgacctatg gaaatggtta ttcacgatgt gtgacagttt
     1261 atcattacac aaaaaatcct actctttttt ggttggccaa tctcatgaac aatcctcata
     1321 cagtggccac actagatgtt agcacagatg aaaagcaaaa agtgattgat caaatcaacc
     1381 gcgcattaga tgaattaaat gatcgatcag ataatgaacg aaaagcgact gaccgaaata
     1441 aaagtgcgga agaatacctg gaactagcgg aatatgcccg aaaattgact tctaatgggg
     1501 aaatttctaa acaagtcaaa gtgagagtac atgtttacgg aaatacgatc caagaagtgg
     1561 atgaacgggc gcaggaactg atcgaaaatc taaaagggat ggattatcgt gcggtgactt
     1621 atctgcataa aaccaaacga gattggcaat cgatgtttac catgttcgat gaacaagctg
     1681 aatggtttgg aaatatcaaa gggcaatcac ttccatcgtt aaacattggc ggaggattcc
     1741 cgttccacca ccaaacgcta aaagatccag gaggcacaca atatggggaa accatgacag
     1801 gcggcccatt tgtctggaat gccacgtata aaaatagtgt acgtttatct accaatacct
     1861 tgcttctagg aatgatggga gctggtaaat cgaccgttct taaaatgatc gcggaagccc
     1921 atttagctgc aggagacttt atttggggct ttgaaaaggg aaaagacttt attcctttct
     1981 taaaggaata taacggtatt atggtgcgtt tagacggatc tgacgggatg atcaatcctt
     2041 tagagatctt tgccacacgg acgtatgatg aagcatccag tttatatgac gatggttcag
     2101 tcaaagattt gaagatcaat gaagcagcgt cctatcaaac gcacttagac aaagtagtct
     2161 atcaggtaca gttagtgagt cctcagctaa aaggaacgat gaaagcagaa ttcaagacct
     2221 acttgaatcg tttttatgaa gagtatggaa cagtaccacg cggttttact tcctcaaatt
     2281 cgcgttcaaa tactgatacg caagtcacag ggaaagatcc agaagcttat ccgacgttca
     2341 aagaattttt agccttttta ggacaactgg aactaccagg ggcttcccaa gagaaaaaga
     2401 atcggaaaga agaaatggag tctatcgtgg aatccttatg tgagacttat ggcatgattt
     2461 ttgatggtca tagtacgatt cgacaccttg accaacaaca gttggtcttt tttgacattg
     2521 actctattac aggcctagat cgaggggtac aacaatgtca gatgtacatg gccatgacgt
     2581 tgatttggaa tcaggcattg attcaaggac gaagacaacg acgcttaatt aaagaaaaac
     2641 aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc cataatggtg
     2701 cgacctgttc tgcagtgaaa agctgtgggt tgcaagtagt ctaaaaacta ttgtaataac
     2761 tcttataacg agaagtcaaa tgagtgggta acgccacaaa gggcttccac tgatactccg
     2821 acatgcaaca agtagtttat gcagaccaac gttgtgtgaa gctcggtgaa gtcggctgaa
     2881 ggataccgta aggtaaaaga aatagtgaat accacgaaag ttctttagag gtaaagggtg
     2941 tatccgataa gtcgggtgtc caaaaaaact taatatggtg agtatgtaca aacgtactga
     3001 cgaactagcg aatgtactgt ccgaaaggca tagctcctag aaatgggaga gacagtgagc
     3061 aaaaaatcac tcattgtgct aacttgtggt tgagtaaaag tcgttgttat gaaagaccat
     3121 gctgcgttaa aggcgtagga gaagttaggg ggcaaaagct agcatctaag ttaaggcatg
     3181 tggatagata taagggaaca ggggaagcca tcaacatgga gagtgcatac tcgatgaagt
     3241 ggtagtaaga aatatgtgaa tgacaaataa cttactttag tgatggtgaa agtagagtca
     3301 cgactgatga aggttctgta atggaactgg aggaacaggc tcgattcgtc tgtaaaagtc
     3361 atgaaacccg ttcaaccatg ggattctcgt atgactaaaa aggtcacgag ccaattggag
     3421 cgtgataaac catgacaaaa gaaacaagtc caaaagatag gaagttgcgg catgcagaat
     3481 attatggcat gacggaaatc ttcgataaga tatactccaa aagtttgaag ggagataatt
     3541 tcaagaatct aatgcagatt atcatttctg ataacaacat tgtcctagca tatcgtaata
     3601 tcaagagaaa tcacggtagt gtgacaagag gagaagatgg agtaacgatt aaagatattg
     3661 aaaatctcaa ccgtaatgaa ttcgtagaaa ttgttaggaa gcgattcgca cattataacc
     3721 ctcggaaagt aaggcgtgta gaaattccta aatataatgg taaattaaga ccgttgggta
     3781 ttccatcaat atgggataga atcgcgcagc aatgcatctt acaagtatta gaaccaatat
     3841 gtgaagcaaa attcaataat cacagctatg gtttccgccc aaatagatca gcagaacacg
     3901 caatagcaga ttgtatggcc cgaatgaatc aaagtcacat ggaatatgtg gtagatgtag
     3961 atatcaaagg tttcttcgac gaagtcaatc atactaaact catgagacaa atctggtcta
     4021 tgggtatacg agacaaacag cttctcttaa tcattaggaa aatgttaaga gcacccgtag
     4081 tacttccaaa tgggaagttg atatacccaa caaaaggaac accacaaggc ggaatactta
     4141 gcccattact tgcgaacatc aatctcaacg aatttgactg gtggatagca aatcagtggg
     4201 aggaaaaaga tgccaaagag ctaaaacatc attttaggaa aaatggagct tgggataaag
     4261 gtaatatcta tcacaagctc agaaagagta caactctaaa agaaatatac ctagtgagat
     4321 acgcagatga ttttaaaatt ttctgtagaa attataatga agcgaaaaaa gttttctatg
     4381 caactaaaat gtggttacag gagcgtctaa agttaccaat atctgaagaa aagtcaaaaa
     4441 tcacaaatct ttcaaaagaa gaaagtgaat ttttaggctt tacgatgaaa ttagtgacca
     4501 aaggtaacaa aaaggttgtt aatacgcata taggtacaaa agcacaaagc atgattaaag
     4561 tgaaactaaa aaaacaaatc aaacgaattc aaaaaagtcc taatagcaaa gataccttta
     4621 tctatattgg agtctataac agtatggtta ttgggattca taattacttc aaaattgcga
     4681 ctcattgtag tgtagcaatg cgaaaaattg caagagaact cagaccaagt tttgaaatac
     4741 gactacgaga acaaggaatc tcaaaagaag gacaatacgg aaagtacgaa ggatacaagc
     4801 cttacctacg gtcaggacaa atacgatata taagagggtt tccaatatta ccagtagggt
     4861 atattcaaca cagacatccg atgtgtaaaa aaagaaatat taataaatac accgaagaag
     4921 gtagggaagc cattcataag aagcaacaaa ccgtagaaga atggaagata tcgtggttgt
     4981 gtagcaatcc agtgcttagt aagagagcaa cgattgaatt tgcggacaat cgaatttcaa
     5041 aattcatcgc tcaaaaggga agatgtgcag taacaggtga ggagcttacc ttatctgaaa
     5101 tgcattgtca tcatattatc ccttatcatg aaagcaaaag tgatagctat gaaaacctag
     5161 tgattgttac tgaggaggtt catcgggtaa tacacgctac acaaagtgaa acgattgagg
     5221 aacttctaaa atatctaaaa ttaaatccaa aacaaaaaga aaaactcaat gagttgagat
     5281 taaaagttgg taacgaagaa atagcttaat aaatatttgt aggacgggaa aacagaatgt
     5341 cttttataga cgagggaacg ccgtatgcgg tgaaagtcgc acgtacggtg tggactgggg
     5401 gaaaagacgg agataatatc aaactcttac ctatcagtat cgattaatcc agatttcatt
     5461 gaaacggtaa attatgtcac aaacttccaa cgagaaatgc gtaaagtaga agcaatgact
     5521 gttttagcta cacaatcccc acaagaaatg gtgccagaaa atatgtcttc tgataacttc
     5581 tcgaaactga agaaagtctt tgaattttct tctacgaaga tctttatgcg tatggatgaa
     5641 tctattttac gtcatataga aggattagtt ggtaaatcaa tgacgaactc agaattagaa
     5701 tccattcctc aacaaaatca aggagacgct gtgatcaatt ttggctcaaa agaaaccatt
     5761 cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg
     5821 aaaaccaaaa tgaaagttta atcaaacagt atgttaagcg tcgtgcaaag cggcgcttat
     5881 ttttgtgggt gtttggtact tctgcaggtt tgattactat attgatcact gtgtttgtga
     5941 ccctttttct aatacttgcg gcaggttcta ttgataattc tgattcaagt agtggtggcg
     6001 aagcttttac aggagaatat tcggaaggct tacccattta taaagaaatc aaaggaagag
     6061 gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaag tataaattat
     6121 taccaagtgt gatcctttcg caatacggtt atgaatcagc gtttggtacg tcggcgtccg
     6181 caagaaatga tctcaattat tttgggatta cgtggtttga tgggtgtctg tttcctaaag
     6241 gaactgcgcg tggaattggt ggcattgaag gtggctggta catgaagttt cccaactcaa
     6301 aagctgcctt ctcctattac ggctttatgg tagccacaca atcaaatttt aatgcttgtg
     6361 tcggaaataa aagccctggc gcaagcttat tgatcttagg acgaggaggg tatgccgccg
     6421 caggaattac tgaagacagt gcctattata aaaattgtat gtctattatt aatacaaata
     6481 aattgatgga gtacgatgag tttgcgatta aacactggag agaaggcggc ggaactggtg
     6541 gtacgattac aggaagctgg ggcaatccat tcccaggaag tagcttagat aaaaattcg
//
LOCUS       DACCSK010000132         6476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCSK010000132 DACCSK010000000
VERSION     DACCSK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.135"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            152..2095
                     /locus_tag="IVP39_002410"
     CDS             152..2095
                     /locus_tag="IVP39_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7587465.1"
                     /translation="MLSKRESQLIELLLEEENFQPASFFSEKLNVSNKTIYQDIQKLG
                     QFLNRFELKIEKKPRMGIYLIGTSTKKNDAKIFITSKEAMSTKVKYSSNYRRVYLFAN
                     LIFKTDKKRYEYFEKKFFVSIATLRKDYEEIIKYCRLAGIRITSNHVIDTQKTPEELI
                     QRTFVNYFEKNIGVGNEKCQYHFVFDQAMVKFVDDFVKNFYQQSKQQPTNYIVHSLKL
                     SLLTLIGRVKHGYHSQTNKNPLFVEIKKMELYMVALDFSNGVKDELNCSFTINDLYFI
                     CSLFMGHGVRPFVNLATHSQQIMETTKQFIQDMSTLVEKDLGQDSQLLQSLFAHIEPM
                     VYRLQMGITIKNPLKEDIIRQYSTMYTLATYCIPKLEKAMGIRVTDDEITFLTIHFQL
                     AFEKIVNTKHVFLICPTGLGTSQLLFQRIRHSSPSTVVYEVIDVQKLQDYNLDSVDLI
                     ISTVKLENEYQTPVIYVRPLPTKEEIALINRHLLTLNQQDNTFLPSIAPDKTILKKYL
                     KDGWMFLNQDFDSRESVLSFITEKYKEEQLVKDNFIEVVLQREKLGATGLSSGVAIPH
                     ANPKYVVKTKISIITLKNKITWGDVDIRVVLFLAIAEEDMIQVKNMIALLYDVFVSPE
                     RIKSIASKETRNEIINEIIGKRK"
     gene            2097..2558
                     /locus_tag="IVP39_002411"
     CDS             2097..2558
                     /locus_tag="IVP39_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7587466.1"
                     /translation="MTSYFGKKIALFQSEVKSQKEALSIIATCLYQTGLVKDSFAENV
                     LKREEVFPTGLLINDVGIAIPHTDAEHVNDSQIAFMSLKTPVTFYEMGTADKEISVSL
                     IFMLALKEPHAQLEMLQKLITMFQKKEVVQKLRAVNNTEEYYQIIEKEGFV"
     gene            2582..3928
                     /locus_tag="IVP39_002412"
     CDS             2582..3928
                     /locus_tag="IVP39_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7587467.1"
                     /translation="MLDLLQKFVDLGSIVVLPILIFVFGLILGTKPSKAFISALTVGV
                     GFVGLNLVIDLLTNSLGPAAQAMVERFGLNLTTIDVGWPAAAAISYGTVLGSLAIPVA
                     IVVNVLLIIVGLTKTLNVDIWNIWHAAFVASLVYAVTNDFAMGMLATVIYTMMILLFG
                     DILGPIINKFYGFPNITFPHGTSAPGFVFALPLNWLFDRIPGLKDWHADPETIQKRFG
                     IFGDSTVMGFLIGLVMGILAGYDVAGIGQLAVKTGAVMVLMPKMVALLMEGLTPISES
                     ANSFVQKRFPGRELYIGMDAALSVGHPAVLSSSLILVPITILLAVILPGNTTLPFGDL
                     ATIPFLICLMAAVFGGNIIRTILGGTIYMVTILYITSWVAPFLTKAAQGANFDLQGHS
                     SVTAMAEGGLWTTLLYVGGAKLFGYFALVGIGLIITGLLFYVNRILPKRAAKQTEA"
     gene            3951..4235
                     /locus_tag="IVP39_002413"
     CDS             3951..4235
                     /locus_tag="IVP39_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7587468.1"
                     /translation="MKKLLIMCGTGVATSTVVTGKVKEWLKSKGLENEVKLYQSKVAD
                     EIGKIDDYDIVVSTTVVPDNIKDKVIMGLPLLTGIGIDGLFDEIEAKIKA"
     gene            4262..5089
                     /locus_tag="IVP39_002414"
     CDS             4262..5089
                     /locus_tag="IVP39_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAP7587469.1"
                     /translation="MLVTTKEMFEKAQKENYAIPAANFFDLDSARTFVEVAEKMQKPL
                     ILAFAQAHIEECPLEEAALIATYLAKKASVPVALHLDHGQDEEIIKQAIELGFTSVMI
                     DASQDEIETNIRRSKAIAEYAHERGVVVEAEIGHVGTGNSYEFKETTDTIYTEVEEAA
                     RFAKETGVDSLAVSIGTAHGQYKGTPKISFERLEEIKNEVSIPLVLHGGSSSGDENLH
                     KCAVSGINKINIFTDFITAAMDVINEEKPEDYFTLKRLANQAIADTLSHYYEVFATK"
     gene            5102..5797
                     /locus_tag="IVP39_002415"
     CDS             5102..5797
                     /locus_tag="IVP39_002415"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7587470.1"
                     /translation="MRTVRKPFFVVNPKSYLYGEQALKLAKTADQLAAAYDIDIIFTC
                     QHVDVRLIKEQTTQLIVSVQHIDGIVPGRGMGHVLPEGVVAAGAEATFLNHAERPLSV
                     SELCDAVKRAKELGLLTIVCANSLEEAKLVASLKPDVMVCEPTELIGTGQASDISYMI
                     QTNEAVRKMDPEIFVLQAAGISTVADVRKALDSGADATGGTSGIVCAKDPVDTLKAMI
                     EEVSKWKKEQSTK"
     gene            5794..6309
                     /locus_tag="IVP39_002416"
     CDS             5794..6309
                     /locus_tag="IVP39_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016380999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP7587471.1"
                     /translation="MITYTEQLVLTSNGQRVTYHNITEQVKEIVAKSGIKNGICLVQS
                     PHTTCSVIFEEFMHDLDFNGDEFLQVDLNRILDRIIPRELTENTDYRYPGPKHLDFLL
                     SLDDPDYAGDAGTILNGDAHIRASFFGASENFIVKEEILMTGTVGSIYFIDFDQNRVR
                     NRKCHILVMGE"
BASE COUNT         2148 a          984 c         1373 g         1971 t
ORIGIN      
        1 ttaaaaaaat tgtcgtgagt tttactgaca tcaaaaagta tagggtcata ataatctgtt
       61 ccacatttat gtggaacaga ttacgtattg atctttagtg agaaaaactc tatgatatag
      121 acaaggaaaa cgttttctga ggtgatgatg aatgttatca aagcgcgaga gtcagttaat
      181 tgaattactt ttagaggagg agaattttca accagcaagt ttctttagtg aaaaactcaa
      241 tgtttcgaat aaaacaattt atcaagatat tcagaagctg ggacagttcc tgaatcgttt
      301 tgagttaaaa attgaaaaaa aacctcgcat ggggatttac ttaattggaa cgtcaactaa
      361 aaaaaatgat gccaaaatat ttatcactag taaagaagct atgtcaacaa aggtaaagta
      421 tagttcaaat tatcgtagag tgtacttatt cgccaatcta atttttaaaa cggataaaaa
      481 aagatatgag tattttgaaa aaaagttttt tgtcagtatt gcaactttgc gtaaggacta
      541 tgaagaaata atcaaatatt gtcggttagc tgggattagg attacttcaa atcatgtaat
      601 agacacacaa aaaacacctg aagagttgat ccagcggaca tttgttaatt attttgaaaa
      661 aaacatcggt gtagggaatg aaaagtgtca atatcacttt gtttttgatc aagcgatggt
      721 taagtttgtg gatgattttg taaaaaattt ttaccaacag tctaagcagc aaccaacaaa
      781 ttatattgtc cattcactca aactttcgtt gttgacgttg attggtcgag taaagcatgg
      841 ctaccattca caaacaaata aaaatccttt gtttgtcgaa ataaagaaaa tggaattata
      901 tatggtggca ctagattttt caaatggggt caaagatgaa ttgaattgtt cctttacgat
      961 aaatgatctt tactttattt gttcgttgtt tatgggacat ggagtgagac cgtttgtgaa
     1021 tctggcaacc catagtcaac aaatcatgga gaccaccaaa caattcattc aagatatgtc
     1081 gacactggtt gagaaggatt tggggcaaga tagtcagctg cttcaatctc tatttgccca
     1141 tattgagcca atggtttatc ggttgcaaat ggggattacc attaaaaatc ctttaaaaga
     1201 ggatatcatt cggcaataca gcacaatgta tacgctagca acttactgta ttccaaaatt
     1261 ggaaaaagca atggggatca gagtaacgga tgatgaaatt acttttttga ctattcattt
     1321 tcagttagct tttgaaaaaa ttgtgaatac gaagcatgtt tttcttattt gtccaacggg
     1381 gttgggaact tcgcaattat tatttcagcg tattcgtcat agttctccta gtaccgtagt
     1441 ctatgaagtg attgatgttc aaaagttgca ggattacaac ttagatagtg tcgatttgat
     1501 tatttcaaca gtcaaattag aaaatgagta tcaaacgccg gttatttatg ttcgaccatt
     1561 accaacgaaa gaagaaattg cattgattaa tcgtcatctt cttaccttga atcaacaaga
     1621 caatacattt ttaccatcca ttgcaccgga taaaacgatt ttaaagaaat atttgaagga
     1681 tggttggatg tttttgaatc aagattttga tagtagagaa tctgtacttt ctttcatcac
     1741 tgagaaatat aaagaagaac aattagtaaa ggataatttt attgaggttg tacttcaaag
     1801 agaaaaacta ggagctaccg gactttcatc aggcgtagcc atcccacatg caaatcctaa
     1861 atatgtagtc aaaacaaaaa tttctattat cactctaaaa aacaaaatta cttggggaga
     1921 tgtggatata agggttgttt tattcttagc gattgcagaa gaagatatga ttcaagtgaa
     1981 aaatatgatt gcactacttt atgatgtctt tgtctcacct gagagaataa aaagtattgc
     2041 aagtaaagaa acaaggaatg agattattaa cgaaattata ggaaagagga agtaagatga
     2101 cgagttattt tggaaagaaa attgcattat ttcaatctga ggtaaagagt cagaaggaag
     2161 ctttatcaat tattgcaact tgtctatatc aaacaggttt agtgaaggat tcgtttgcag
     2221 agaatgtact gaaaagagaa gaagtgtttc ctactggctt attaatcaat gatgttggga
     2281 ttgctatacc acatacggat gcagaacacg tgaatgattc tcaaattgca tttatgtcat
     2341 tgaaaacacc agtaactttt tatgaaatgg gaactgcaga taaggaaatt tcagtcagct
     2401 tgatcttcat gttggcgttg aaagaaccac atgcacagtt agaaatgtta cagaagttaa
     2461 ttacgatgtt tcaaaaaaaa gaggtggttc aaaagcttcg agctgtaaat aatactgagg
     2521 agtattatca aattattgaa aaagaaggat tcgtttaata accattttga gggggagcat
     2581 tatgttagat ttattacaaa aatttgttga tctcggatca atcgttgtat tgccaatctt
     2641 aatttttgtt tttggtttga ttttaggaac aaaaccgagt aaggctttta tttcagcatt
     2701 aacggttggt gttggatttg tcgggttgaa tttagttatc gatttattga ccaatagctt
     2761 aggaccagca gcacaagcga tggttgaacg ttttggacta aacttaacaa ccattgacgt
     2821 tggttggccg gcagcagctg cgatttctta tggaaccgtt ttaggtagtt tggcgattcc
     2881 agtagctatc gtagtcaatg tgttattaat tattgttggc ttaacaaaaa cactgaatgt
     2941 tgatatttgg aatatctggc atgcagcatt tgttgcttca cttgtctacg cagtaacgaa
     3001 tgactttgca atgggcatgt tggcaacggt tatttataca atgatgattt tattgtttgg
     3061 cgatatttta ggaccaatca tcaataaatt ctatggattt ccaaatatta ccttcccaca
     3121 cggaacatca gcacctgggt ttgtttttgc cttaccttta aattggttat tcgataggat
     3181 tccaggattg aaagattggc atgcagatcc agagacgatt caaaaacggt ttggcatctt
     3241 tggtgattca acagtcatgg gctttttaat tggtttggtt atgggaattt tagctggcta
     3301 tgatgttgcg ggaatcggtc aattagcggt gaaaactggt gctgtaatgg tcttaatgcc
     3361 taaaatggtg gcacttttaa tggaaggctt aacacctatt tcagaatcag caaattcatt
     3421 tgttcaaaaa cgtttcccag gaagagaatt atatatcgga atggatgccg cattgtcagt
     3481 aggacaccca gctgtattgt cttcttcgtt aatcttagtc ccaatcacga ttttattagc
     3541 agtgatttta ccaggtaaca caaccttgcc tttcggtgat ttggcaacta ttccattctt
     3601 aatttgtttg atggcagctg tctttggtgg aaacattatt cgaacaatct taggtgggac
     3661 gatctacatg gtcaccattt tatacatcac ttcatgggtg gcaccatttt taacaaaagc
     3721 agcacaaggg gcaaactttg atttacaagg acattcatca gtaactgcga tggcagaagg
     3781 tggactgtgg acaacacttc tatacgtggg tggtgcaaaa ctatttggct actttgccct
     3841 agtcggaatt ggtctgatta ttacaggctt actattctac gtgaatcgca ttttacctaa
     3901 aagagcagct aaacaaactg aagcttaata caaaatagga gagatgacag atgaaaaaat
     3961 tattaattat gtgcggaaca ggtgtggcta cttcaacagt ggtaacagga aaagtaaaag
     4021 aatggttgaa atcaaaagga ctcgaaaatg aagtgaaact atatcaatca aaagttgctg
     4081 atgagattgg caaaattgat gactacgata ttgtggtcag tacgaccgtg gttcctgaca
     4141 acattaaaga taaagtaatt atggggcttc ctttattaac cggaattggc attgatgggt
     4201 tgtttgacga aattgaagcg aagattaaag cctaaaaaat acttgaaaag aaggtaatca
     4261 aatgttagtt acaacgaaag agatgtttga aaaagcgcaa aaagaaaact atgcgattcc
     4321 ggcagcaaac ttttttgatt tagattctgc aagaaccttt gtagaggttg cagaaaaaat
     4381 gcaaaagcca ttaattttag cctttgcaca agcgcatatt gaagaatgtc ctcttgaaga
     4441 ggcagcgtta attgcaacat atttagccaa aaaagcaagt gttccagtag cacttcattt
     4501 ggatcatgga caagatgagg aaatcatcaa gcaagcaatc gagcttgggt tcacttctgt
     4561 gatgatagat gcttcgcagg atgagattga aacaaatatt cgtcgttcaa aggcgattgc
     4621 ggaatatgct catgaaagag gcgttgtggt agaggctgag attggacacg tgggaacggg
     4681 aaatagttat gaatttaagg aaaccacgga caccatttat accgaagtgg aggaagcagc
     4741 acgttttgcg aaagaaaccg gcgtcgattc attggcagtt tcgataggta cggctcatgg
     4801 gcaatacaaa ggaacaccga aaatcagctt tgagcggttg gaagaaatta aaaatgaagt
     4861 ttcaattcca ttagtgttgc atgggggctc atcatctggt gatgaaaatc tccacaaatg
     4921 tgctgtttca gggatcaata aaattaatat ctttactgat tttatcacag cagcaatgga
     4981 tgtaattaac gaagaaaaac cagaggatta ttttacactt aaacgattag cgaaccaagc
     5041 gattgcggat acgttatcgc attattacga agtatttgca acaaaataga ggagggttct
     5101 catgagaaca gtaagaaaac cattttttgt ggttaatcct aaatcttatt tgtatggtga
     5161 acaggcattg aaactagcaa aaacagcgga tcaattagct gcagcatacg acatcgatat
     5221 catttttact tgtcaacatg ttgatgttcg gctaattaaa gaacagacaa cgcagttgat
     5281 tgtgagtgtt caacatatcg atgggatagt ccctggtaga gggatgggac acgttttacc
     5341 tgaaggcgtt gtagcggctg gagcagaagc gaccttcctt aatcacgcag aacgtccttt
     5401 atcggttagt gagctgtgcg atgccgtcaa gcgagcaaag gaacttggtc tattgactat
     5461 tgtttgtgca aattctttag aagaggctaa gctcgtagct tccttgaaac ctgatgtgat
     5521 ggtttgtgag cctacggaat taataggtac aggtcaagca agtgacattt cttatatgat
     5581 tcaaaccaat gaagctgtac gcaagatgga tcctgaaatt tttgttctgc aggcagcagg
     5641 gatttcaacg gtagcagatg ttcgcaaagc acttgattcg ggggctgatg caacgggtgg
     5701 aaccagcggg attgtttgtg ccaaagatcc tgtggacaca ttgaaagcga tgattgaaga
     5761 agtaagtaaa tggaaaaagg agcaatcgac aaaatgataa cttatactga gcaacttgtt
     5821 ttgacttcaa atggacagcg agtgacgtat cataatatta ccgaacaagt aaaagaaatt
     5881 gtggcaaaaa gtgggataaa aaatggcatt tgtcttgttc agtcgccaca tactacttgt
     5941 tcagttattt ttgaggaatt catgcacgat ttagatttta atggggatga atttttacag
     6001 gttgatttaa atcggattct tgatcgaatc attcctcgag aattaacgga aaataccgat
     6061 tatcgttatc ctggtccgaa gcatcttgat ttcttgctgt ccttagatga tccagattat
     6121 gctggtgatg ctggaacgat tttaaatgga gatgcacata tccgagcatc attcttcgga
     6181 gcaagtgaaa atttcattgt caaagaggaa atactaatga caggaactgt cggctccatt
     6241 tatttcattg attttgatca aaaccgtgtt agaaatcgaa aatgccatat tttagtaatg
     6301 ggtgaataga aaagtaataa gaaacaaaca tttgggaaac taattgaatg atcacaccta
     6361 taaaaattaa tacgaaagat aatagagtca agtccagact cctgtgtaaa atgctataca
     6421 atgtttttac catttctact tatcaaaatt gatgtatttt cttgaagaat aaatcc
//
LOCUS       DACCSK010000133         6425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCSK010000133 DACCSK010000000
VERSION     DACCSK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.136"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(3..680)
                     /locus_tag="IVP39_002417"
     CDS             complement(3..680)
                     /locus_tag="IVP39_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7587472.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(798..1373)
                     /locus_tag="IVP39_002418"
     CDS             complement(798..1373)
                     /locus_tag="IVP39_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7587473.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            1504..2208
                     /locus_tag="IVP39_002419"
     CDS             1504..2208
                     /locus_tag="IVP39_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7587474.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            2186..2983
                     /locus_tag="IVP39_002420"
     CDS             2186..2983
                     /locus_tag="IVP39_002420"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7587475.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            2985..3884
                     /locus_tag="IVP39_002421"
     CDS             2985..3884
                     /locus_tag="IVP39_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7587476.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            3902..5263
                     /gene="mgtE"
                     /locus_tag="IVP39_002422"
     CDS             3902..5263
                     /gene="mgtE"
                     /locus_tag="IVP39_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7587477.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(5325..5966)
                     /locus_tag="IVP39_002423"
     CDS             complement(5325..5966)
                     /locus_tag="IVP39_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7587478.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            6075..>6425
                     /locus_tag="IVP39_002424"
     CDS             6075..>6425
                     /locus_tag="IVP39_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HAP7587479.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQ"
BASE COUNT         1941 a         1176 c         1392 g         1916 t
ORIGIN      
        1 ttttaattag aaagcaagtg taaatggcta gttcttgaat ggaactgaga atcatttgta
       61 tgtaaatgtt caccattaaa aacttgatat gagttatctg ttgttttaca cagctctgtg
      121 attctccaaa ggctacttgt accttttact tctactaaaa caccaaaatc acggtcgcag
      181 gcataattgt aaacgactgc agaagggtgt tttgtgatgc ccatttcccg agcaattttt
      241 tgatcagaaa agaatacttc tttaacgaag tcagactgac gatcttcttt aaacatattc
      301 aaatcgccgc cgatttcttc aaatatggat tcagcaagtt cttgagaata tggtatgtca
      361 ttacagccga ctgcttcttg caatttgatc aaaaaacggc ggcctttttt ctttccttga
      421 aactgcgccg ctttgcagtc caatgctgca gaataagtca ttgagaataa gtgattacgt
      481 tcctcaagat tgttctgaga aattcctttt tgttgcatga catccccaat tgtctgcaaa
      541 ttgaccaaag gaagaaagcg aaattgcatc ttttcttttg atggtgtccc gtactcttct
      601 tcgataaatt gtaataactg ttgttccatt tcgaaacagt attcccctaa aggattaaca
      661 aataaataga tttcgatcat atgaatctcc cccaaaatag attctataca actagtgact
      721 agattctttc acatcctata acttaacaga aaaaagtaaa aaaatgaact caagtgcctt
      781 gtgaaaatga aaggtttcta atttttagct tcaagcatac gttggatctt gttcttagca
      841 ggacgataag gtaaagaaaa acgttttaaa aaatttataa aagcaatttt tcccacagat
      901 gcatccccta cctctagttc cagttcaaaa tcgtgtagat tgtctcccca gctttcatca
      961 atagctaaaa gtccttcttc aatagggaat tcagctcgtt ttgttgtaag ctttccggtt
     1021 tggattaatg aagcaggatc gatattcaaa tcagaaagtt tttgaaaaac tgcgcctgaa
     1081 cgaggcagac gtttttcgaa gataaatgct tctgcttctt ctgttataaa ggtatccgtt
     1141 gtttcgagac gccctttttc agcaggtgtt tttaatgtat attcagcttg atgaccaacg
     1201 aaacggatcc gtaggccaca attttgtttc ttcagctgtt catcaacggt atcgaaataa
     1261 atattagtct gtacacggaa atctttatct gttaattgat agtgagcgat gaatatctcg
     1321 tactcttgct ttgtcagcat agatttatat tcgatttcaa gcatttgtgt catttgatcg
     1381 actccttttt gtctattttg cctaattttc tttttttggt caatgcataa atcaagagta
     1441 cgaaaaaact ttgcatatat ggtagaataa acaatgaatg ttaatgaagt agaggtatca
     1501 gctatggaac gagattggga agcattttta gcaccatatg aacaaactgt atcagaattg
     1561 aaagtgaaac tgcgggggat ccgcaaacag ttccgtgaac aaaatcgaca tgtcccgatt
     1621 gaatttgtca ctgggagagt caagccagtc gatagtattg taacaaaatc acaattacgg
     1681 catattccaa tgggacgttt agaagaagaa atgtctgata ttgccgggtt aaggatcatg
     1741 tgtcagtttg tagaagatat ccatcaagtt gttgagatca tccgtaaaag aaaagacatg
     1801 aaagttctcc aagaacgaga ttacatcaca aataaaaaag caagtggcta ccgttcttat
     1861 catctggtga tcgagtatcc tgtacagctg atcagcggtg agaagaaaat tttggcggaa
     1921 atccagattc ggactcttgc gatgaatttc tgggcaacga ttgagcattc gctgaattat
     1981 aaatatcaag gcgtttttcc agaagaaatg agtgaacgac ttcagcgtgc ggcagaagct
     2041 gcctatcagt tggatgaaga gatgtccagt atccgtgaag aaatccaaga agcacagcgg
     2101 ttattctccc atggaagagg aaaatcagat gatgtctact atcgaacgct tttgaagaaa
     2161 gaaagcgaaa gagaggaggc aggggatgaa agtagcgatc gttcataata aagaagcaaa
     2221 gactatagaa gtgacagaac gtttgaacgc acttttagaa caagcaggga ttcaacgaga
     2281 tgatcatgag ccagaattgg tcatctcagt cggcggagac ggcacattat tgtcggcgtt
     2341 ccatcactac agtcattgtc tgaatgatgt gcgtttcttg ggcgtgcata caggtcattt
     2401 agggttttac acggattggc gggattacga gctggaagaa ctagtagaca gcttacgaac
     2461 gaaccgggaa caaagtgtta gttatccctt gctggatgtt cggatcagtt atttggatga
     2521 aacaccggac cagcattttt tagcactgaa tgaatcaacg attaaacggg ccaatcgtac
     2581 gatggtagcc gatgtataca tcaaaaacga gctgtttgaa agttttagag gagatggatt
     2641 gacgatttct acgcctactg gttcgacagc atataataaa agtgtcggcg gtgctgtgat
     2701 ccatcctagt atcaatgcct ttcagttggc agagattgct tcactgaata atcgagtgtt
     2761 tcgtactttg ggttctccga tcgtgattgc ccatgatgaa tggctagaaa taaaattgga
     2821 aaatacagaa gattatcttg taacggttga tcaattgaat gtcgcaaaag caaatatccg
     2881 ctcaatttat tatcgaatag cagaagaacg gatccatttt gcttcttatc gacatatgca
     2941 tttttggcat cgtgtgaaag atgcctttat tagtgaggat tgatatgcag tttacatggg
     3001 attatgagaa agatcattct cagcaagtaa aatatttttt aaaagaaaaa gggatctcaa
     3061 agggtctgtt agcgaaaata aaatttcaag gcggtcaaat caaagttaat gatcaagtag
     3121 aaaatgtctt gttttctcta gctaaggatg ataaggtaac cattgtgatt cctgctgaag
     3181 gagaacatga aaccgtactt ttggatgaaa caccgatcga tatcgtgtat gaagatgaac
     3241 acgttctggt agtaaataaa ccagcaggcg tttcgtctat tcctgcccaa taccatccaa
     3301 atgggacaat ggctaatcgt gtaaaggctt attataaaaa acaaggttac gaaaaccaag
     3361 taatccatgt ggtgacgcga ctagatcggg atacatctgg cttgatgctt tttgcaaagc
     3421 atggatttgc gcacgcaaaa ttagatatgc aacttcgtga aaagaagttt gtgaaaaaat
     3481 atcaggcctt agtcagtgga aggctttcag atttatcggc acatggacat atcgatttgc
     3541 cgatcgcccg agattattct tcgctattga agcgaatggt ctctgatgaa ggacgttcgg
     3601 cacagactga gtactggata gagaagcgac taacagaaat cgcgttagtt gatatccagc
     3661 tgcatacagg aaggacacat caaataagag ttcatttttc cgcaattggc tgtgccctgc
     3721 tcggtgatga tatgtatggc gggcgaatgg atctgggaat aaaaagacaa gcccttcatt
     3781 gttatgatct gcgtttttac catccattta cagaagagtt gctggagttc aagcagcctt
     3841 tagccgcaga catggaggca gttgtgagac ggtttgaaga ataaggcgag gtgagattgg
     3901 tttggatgaa aatcaagaat tagaggaaag atttgcccag ttgactcaag atttaaggga
     3961 aaataatatt caggcattca gaaacagttt tttagagatg catatctatg agcaggggca
     4021 attctaccaa tctcttacag aagagaacag gcagctgatc tattcctatc tgtcgcctaa
     4081 agaattagcg gatatgttcg atgtgattga agaagacaac gaacatatga aagaatacct
     4141 agatgaaatg cgtcctagct atgcagctga tatgctttct gagatgtata cagataatgc
     4201 tgttgacttg cttaatacgc tggataaaaa gcaaattgct aaatatttaa gtttgatgtc
     4261 ggcagacgat gccagtgaaa tcaaggaact tctccattac gaggatgaaa cggctggcgc
     4321 gatcatgaca actgaatttg tttcgatcgt agccaatcaa actgttcgct ctgcgatgta
     4381 cgtgctgaag aacgaagcag atgtagctga aacaatctat tacatctatg tcgttaatca
     4441 agaaggtcag ctagtagggg ttatttctct gcgcgaccta atcgtgaatg atgacgacgc
     4501 aatgatttct gatctgatga gcgaacgagt gctttcagtt catgtaggcg acgaccaaga
     4561 agatgtggca caaacattta gagattatga cttcttagct cttcctgtta ccgattatga
     4621 tgatcatttg ttaggaatcg tcacagtcga tgatatcatc gacgttattg atgatgaagc
     4681 tgctagcgat tattcaggac ttgccggggt caatgtggaa gaaattaatg aaaatccagt
     4741 aaaggcagcg tctcgacgtt tgccttggct gatcactctg ttgtttttag gtatgtcgac
     4801 cgcttcttta atcagtcact atgaagactt ggtaagtgaa gcgagtattt tagcagtctt
     4861 catttcgctg attacaggaa ctgccggtaa tgctggaacc cagtcgctag ctgtggctgt
     4921 tcgtcgactt gcgatttctg atgagaaaga tcatggattt gttcgacttg ttttggctga
     4981 agtcttgacc ggcgttgtca caggagctgt tacggggatc acgatttttc taatcgtagg
     5041 tttttggaaa cataatttta tcttggggtt tgtcataggg atggctatgc tatttgcaat
     5101 cacagtggcg aatcttgcag gtagtttaat cccgatgcta atggatcgtc taggatttga
     5161 tccagcagta gcaagcggtc cctttatcac gactttgagc gatttgacga gtgtactgat
     5221 ttatttcaat attgcttctt tatttatggg ctattttatg taaaaaaaca aaaacatttg
     5281 caataagcat tatgcctgtt gcaaatgttt ttttgtttat aagattataa ctgaagatcc
     5341 acgattttgg ttccgtgtac ttcgcttacg ttcaattctt tagctacatg tgctgcattc
     5401 tctgcagtga caccaccacc aggcaaaatg attaagcggc catctgtata ggcaatcaat
     5461 tctttaagcc gtgggaaatt ttctacaata gttttgtcag caggaccgcc atgagtcaag
     5521 atacggtcaa caccatgttc taccagccaa tcgattgctt cgaattgccg atcttcagga
     5581 atactgtcaa aagccatatg gaaggtgatc tgcaaccctt ctgctgtttc gatcaatagt
     5641 tctaaggcct cttcgtctag ccagcctgaa ggagtaagac aaccaaatac tacgccatcc
     5701 gtccctaatt tctttgcttc aatcaaatca gtatgcatga ttttcaattc gatatcgtta
     5761 tagacgaagt cgccccctct aggacgaata atcgtcatta cggggatatc tttttcccca
     5821 gcataactta acgtttcttc gatcacacca gtactcgggg ttgtgccacc gaccgctaag
     5881 ttgtcacaca attcgatccg atcggctcct ctagcaattg ctagaggaat agatgtgtag
     5941 ttttcagcgc aaaattcttt gatcatctaa tcttctcctt tatctttcac tttcttctag
     6001 gcattgtatc atatttttag cagttgccaa agtgagcatc taacggtaag ataaaacaga
     6061 aatgaggggg aactatgtta aaaaaaattg accgaggacg ccagtttttg gcagaaaatc
     6121 atcagctgtt ccgctgtccg gtttgccaag aagaagtaaa aaagtctgat aatggcttga
     6181 tatgcaaaaa taatcaccgt tttgaccttt ccaaaaaagg aacactttat tttttatccc
     6241 atgcggttaa aacagaatac gatcaaggaa tgttcgttcc aaggcgtaga atgatccaat
     6301 ctggcatgta tcagcccgtt attgagacta ttgcgcagca tttacctgaa gaagtctcgc
     6361 tattagatgt tggctgcgga gaaggtagct tcttgaatga actttcaaaa ttgaaaaaga
     6421 ttcaa
//
LOCUS       DACCSK010000134         6052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCSK010000134 DACCSK010000000
VERSION     DACCSK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.137"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IVP39_002425"
     CDS             complement(138..428)
                     /locus_tag="IVP39_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587480.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IVP39_002426"
     CDS             complement(546..806)
                     /locus_tag="IVP39_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587481.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IVP39_002427"
     CDS             complement(943..1356)
                     /locus_tag="IVP39_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7587482.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IVP39_002428"
     CDS             complement(1812..2297)
                     /locus_tag="IVP39_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587483.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IVP39_002429"
     CDS             complement(2436..2651)
                     /locus_tag="IVP39_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7587484.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IVP39_002430"
     CDS             2875..3675
                     /locus_tag="IVP39_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7587485.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IVP39_002431"
     CDS             3694..5562
                     /locus_tag="IVP39_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7587486.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKVGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IVP39_002432"
     CDS             5555..6046
                     /locus_tag="IVP39_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7587487.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
BASE COUNT         2228 a          952 c         1088 g         1784 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aagtgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa at
//
LOCUS       DACCSK010000135         6038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCSK010000135 DACCSK010000000
VERSION     DACCSK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.138"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            127..801
                     /locus_tag="IVP39_002433"
     CDS             127..801
                     /locus_tag="IVP39_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7587488.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            798..1139
                     /locus_tag="IVP39_002434"
     CDS             798..1139
                     /locus_tag="IVP39_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587489.1"
                     /translation="MMEIRWAVKGDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            1169..1528
                     /locus_tag="IVP39_002435"
     CDS             1169..1528
                     /locus_tag="IVP39_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7587490.1"
                     /translation="MSCLGNVIWFIFGGLIGGLIWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHIVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            1722..1910
                     /locus_tag="IVP39_002436"
                     /pseudo
     CDS             1722..1910
                     /locus_tag="IVP39_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2052..4136
                     /gene="cadA"
                     /locus_tag="IVP39_002437"
     CDS             2052..4136
                     /gene="cadA"
                     /locus_tag="IVP39_002437"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7587491.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            4447..5913
                     /locus_tag="IVP39_002438"
     CDS             4447..5913
                     /locus_tag="IVP39_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7587492.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         1981 a          970 c         1291 g         1796 t
ORIGIN      
        1 aattgtttga agggactagg ctggtgtcat tttttataca tatattttct ctttcgtctt
       61 gatagggctt atgggataat ataaggaaga aaatcatcgt tttattcaaa aaggagtttt
      121 tgacaaatga aaaaacgtat attatttatc ggtgatagca ttacagacgc aggaagagac
      181 cgagcagaag caactgatct aggtaaaggt tatccacttt tgattgccgg aaaactaatc
      241 tcccaatatc caacgatgga atttgaatgc atgaaccgtg ggataggagg agacaaaatt
      301 gcagatctgc aagtacgctg gcaagaagat tgtttagctt tacaacctga ctatgtatcg
      361 attttgatcg gaatcaatga tacctggcat aatactagcg atcaggctat attcggaact
      421 gagaaatctg ctcagcaatt tgaatccgtt tatcgaaaat tactaaatga acttcaaaca
      481 gcggaaatct gcaacatcat cttgatggag ccgtttgtac ttcctataac cgacgaccgt
      541 catggctggc gtaaagactt agaccctaaa atccaaattg ttcgtaaact tgctaaggaa
      601 tatggcgcat tgctggttcc tttagatggt atattcaatg ctttagggat tgcttatggt
      661 ttttcgaaat atacaagaga agatggggtc cacccaacga ttggcggaca tgagatcatt
      721 gcacaaagct ggatagatgc aattgccgca gtcgattttt ttagtggaga taaaaatgag
      781 cgaaagaaaa gtgaaaaatg atggaaataa ggtgggcagt aaaaggagat ttacctcaaa
      841 tcaatcagct gattactgat catccagaat cattcgtaaa agcaaaaacg gcagctgacc
      901 taaatgataa gaataaacta ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat
      961 ttgcgcaagc ggacgtgcaa cagtcagaag aacgattgga tcagcgacca tcaggaaaac
     1021 taatccaaca cacattagta acaaaagaaa atacggaaga aatccaaaaa aaatttgcag
     1081 aatacttgat gctggaagct aaaaaaagaa gaataaaatc aatttttgcc cagttttaat
     1141 caaaaacagt aatagaagga gaaatacaat gagttgttta ggaaatgtca tctggtttat
     1201 attcggcgga ctaatcggtg gtcttatctg gttagcagcg gggatacttt ggtgtatcac
     1261 gatcataggt atcccaatcg gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc
     1321 ttttggcaaa gaagtaattt atcaccagtc tggcatgtca ttgatcgtta atatcatctg
     1381 gctgatcgtt tcaggcctac caattgctct cgcacatatc gtcagcggca tccttctatg
     1441 tatcacgatc atagggatac cttttgccaa gcaatctttc aaacttgctc gattggcgtt
     1501 gatgccattc ggtgcagaaa ttgtataaaa gagaataata aaggttagat aaagcataga
     1561 gtcaggtggg atacatgtat ttcacctgac tctatgcgtt ttcctgtata agtttttcca
     1621 tgtgactttt acgtctttta tttatatggg gttactgttt atggaaagtt gaaataattt
     1681 tcattaaaga actctttcta tatttttgaa agcgtagtgg agcttcgtat atctgggaag
     1741 gacattttgc agggattgaa aaaagatatt gtcctatcga aaaattaatg aatttagaca
     1801 aattgccagc aacaggcttc aaacttagtt gctatcctgt caagatcaag aaggcaagtg
     1861 cgggttggat aagagcagtg gcaatgatcg aagagaaaaa gaaagaataa gccaaaacat
     1921 ctgtatggaa gaaattttcc gatgcagatg tttttcattt tttctttcgg ataccttgac
     1981 atatgaataa ccattcattt ataattagtt atgaagagat attcatatgt tgaggcgagg
     2041 aggtcattaa aatgaaaagt tacagattag aaggtttaga ttgtgcgaac tgtgcaatga
     2101 aaatcgaaaa aggagttcag aaaatcaatg gagtcaagga agcaacggta aacttcactt
     2161 ctggaaaact aacgattgac gcagaggaag atcatttggc tacgattgaa caagaaacaa
     2221 aaaaagtagt gaaggaatta gaaccagatg tcaaagtaac agaaatagat aaggaaaaag
     2281 tatctgaaca tggaaatgaa aaagagagaa atacgctgtt ccggatttta ttttcccttg
     2341 caggtatcgc tttattgcta ttgtttgatt ttaatgaacc aattcgcttg atagggtatt
     2401 tattgattta tctgctgata ggatatgatg tagtgaagaa ggcagtgatg aatatcgtca
     2461 aaggaaaaat ctttgatgag aattttctta tgtctgttgc tacgattggc gctatgttga
     2521 tcggtgaata tccagaagcc gtagcagtga tgctatttta tcaaatcggc gagtatttcc
     2581 aaggattggc agtcagtcat tcaagaaaat cgattcgcga attgatggcg atccgtccag
     2641 aaacagctca cgtgcagacg gcggaaggat tgatgaccgt gaaccctgaa gacgtgctga
     2701 tcggtcagtt tgtacttgtg aagcctggtg aacgtgtacc gttagatgga acgatcatcg
     2761 aaggagaatc attagtcgat acgtcagcac ttacaggaga atcagtccct aaatctgtat
     2821 atgttggtga aacagtatta agcggattta tcaataaaaa taaaccattt ttagttcaag
     2881 tagaaaaatc ttatgaaaat tctactatca gcaaattatt agaattagta gaaaatgcca
     2941 gcagtaaaaa agcaccagct gaaaatttca ttactaaatt cgcgcgctat tacacaccag
     3001 tcgttgtagg attggccgtt ttattagctg ttttacctcc acttgttgtt tctggagcgg
     3061 cgttttctga atggatctat cgagcattga catttttagt gatctcatgt ccatgtgcat
     3121 tagtgatttc cgtgccactt agtttctttg gcggtatcgg aggagcaagt aaaatcggtg
     3181 tattagtaaa aggttcaaat tacttggagt tgcttgcaca aactgagaca gtggtattcg
     3241 ataaaactgg cacattaacg aaaggtgact tttcgatcca gacgatcgat ccaacaggaa
     3301 ttgatccaaa gaaatttatg caatatgttg caagtgcaga acagttttct acacatccga
     3361 ttgctcaatc tgttttagaa ggctacgcag gtccgctttt accaacagct aatatccaag
     3421 aatttgcagg tgaagggatc ttagctgaag ttgatggcaa acaagtatta gtaggtaatc
     3481 acaaattgat ggaaagattt gaaatctctt ttccttcaag tcaagaaatc ggtacattgc
     3541 tttatctagc gatcgatcaa agttacagtg gttatcttgt tattgcagat accttgaaag
     3601 aagatgccgt agacgcattg gttcaattaa aacaggctgg tgtgaagaac acagtcatgt
     3661 tgacgggcga ctcgaaaaaa attgctgatc atatcgggaa gcaagtaggc gtggacaaga
     3721 tatatagcga attgcttcca gaagataaag tgcagcggtt agaggaaatc ttacaaagta
     3781 acaataaaaa gaccgcattt gtcggagatg gaatcaacga tgcaccggtg ctagcaagag
     3841 cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg
     3901 tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa
     3961 cattgaaaat cgtcaaacag aacattgtct ttgctattgg gataaaaatt ttggtacttt
     4021 ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt atttgcagat gttggggtga
     4081 cagtattagc tgttttgaat gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa
     4141 tgaagaggac gaatgaacgt cctctttttt ttatatcctt ttcttaatga aaaatgtaaa
     4201 gaaaataaat aggattataa aataactaat gtttttaatt ttgcagaaaa ttcaaaatag
     4261 cttgaaattc aataaggtca atgaaataaa agaaaataac ttattttttt gatggaaaaa
     4321 atatgaaaaa acgatgtgtg ttaaataaaa gaatacacac ataaaataac actgtcttgt
     4381 aagtttgtta aaaattgaat atgattacat ctggataaca aatttttatc ataaaggaga
     4441 gaacgaatga acaacaaaca attacgctgg tttactgttg cgttaattgc atttaacatg
     4501 gtttggggca tggggaatgt agttaataac tatgctcagc aaggagtatc agttgtcacc
     4561 tcttggctat tgattttggc catttatttt attccatacg cattgatcgt tggacaatta
     4621 ggatcagcct tcaaggatag taaaggaggc gtcagttcat gggtcgaaaa tacgacatct
     4681 aataaacgac tggcatacta tgccgcttgg acttactggg tcgtccatat tccttatttg
     4741 gcacaaaagc ctcaagcaat tctgattgct ggtggttggg cagtccaagg aaatggaaat
     4801 atcgtaaata cgatgtcagt acaaatggta gcagttattt cacttattat atttcttgcc
     4861 tttttatatt tatcaacaaa aggactttct actttgaaag tgattggcgg actggccgga
     4921 acagcgatgt tcgtcatgtc gatcttattt attcttttag ctgtaacggc accgttcatc
     4981 aatccaacga tggaatttgc cacaccacat atgaatcaag tgaaaacata tattccaaca
     5041 tttgatttca gttatttcac aaccgtttcg atgctagtat ttgcagttgg aggggcggaa
     5101 aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa aggaatttcc taaagggatg
     5161 atcttcttag cagcaatggt agggatttgt gctgtattag gctcagtcgc aatgggtatg
     5221 ctgtttgcta gcgataatat ccctaaagac ttgatggcaa atggggcata cagtgctttt
     5281 caaattttag gtaattacta tggtatcggt aactcattga tgatcattta cgcattgaca
     5341 aacatgattg gacaaatctc cgcattagcc ttttcaattg atgcaccact aaaaatcttg
     5401 ttagcagatg cggatccaga atttgtacca aactggctac gtaaacgtaa tcgtaaaggt
     5461 acattggtga atggttatct tctgacaggt atactagtag gaatcattat tttgttgccg
     5521 atctttggta tcaaagatat gaatgaatta gtaaaatggt tgacgaactt gaactcagtt
     5581 gtcatgccaa tgcgttacct atgggtgttc ttggcatata tgatgttgaa taaagcttat
     5641 aagaaattca attctgaata taaatttgtt aaaaatccaa aaatcggttt tatctttggt
     5701 gcttggtgct ttgccttcac tgcgtttgca tgtatcctag gaatggtgcc aaaagtagat
     5761 tatactgctg atcctaaagc atggtggttc caattattca cgaatatcat cacaccaatt
     5821 gtcttgatct tattaggtat gatcttgcca gcaattgctc gtcgagacaa aaacaaagaa
     5881 atcactgaac cagaaacagt ggttcagcct taaaatagaa atacttgtta caagaggttg
     5941 tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaatagc ttctcaaatt
     6001 tttgtgaagt ttcggcttgt ttttcgaaga agctactt
//
LOCUS       DACCSK010000136         5991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCSK010000136 DACCSK010000000
VERSION     DACCSK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.139"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            175..792
                     /locus_tag="IVP39_002439"
     CDS             175..792
                     /locus_tag="IVP39_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7587493.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            833..1132
                     /locus_tag="IVP39_002440"
     CDS             833..1132
                     /locus_tag="IVP39_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587494.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1166..1333
                     /locus_tag="IVP39_002441"
     CDS             1166..1333
                     /locus_tag="IVP39_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7587495.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1378..1983)
                     /locus_tag="IVP39_002442"
     CDS             complement(1378..1983)
                     /locus_tag="IVP39_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7587496.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            2108..5095
                     /locus_tag="IVP39_002443"
     CDS             2108..5095
                     /locus_tag="IVP39_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP7587497.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            5260..5670
                     /locus_tag="IVP39_002444"
                     /pseudo
     CDS             5260..5670
                     /locus_tag="IVP39_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            5804..>5991
                     /locus_tag="IVP39_002445"
     CDS             5804..>5991
                     /locus_tag="IVP39_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7587498.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKL"
BASE COUNT         2080 a          980 c         1105 g         1826 t
ORIGIN      
        1 aaatataatc atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc
       61 ttgattttct ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg
      121 atttaatctg attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga
      181 aaattttttt atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc
      241 gcgaaggaat taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat
      301 tttaaacgcc ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat
      361 ctacaatcat tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa
      421 ttaactaagg aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct
      481 agttacaagc aactagatgg gttagagacg cttatctctg acttaattct gcaattactc
      541 agctatatgg ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa
      601 gctgcgaaac aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt
      661 cctgcgatct attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa
      721 ttgggcatga aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac
      781 cacttttctt gatagatata acagctatta gacgggaagg gagagtttga aaatggactt
      841 aacgaatcaa agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat
      901 cggtttggtt cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt
      961 cctggtttgt ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga
     1021 acaaaccgcg cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga
     1081 attttatgcg ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt
     1141 aaaatttaca aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga
     1201 aataactagt tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc
     1261 aaaaagtgtt tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt
     1321 acatggaaca tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta
     1381 caaatcattc gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc
     1441 aatctctgca actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg
     1501 tttttgcgga tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc
     1561 tttttgagcc aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg
     1621 tgcaatataa cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt
     1681 tccagtatta aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt
     1741 cataaaagca actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt
     1801 aacgatgacg caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc
     1861 agtatcttta ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat
     1921 tgccaattgt cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc
     1981 cataaaaaac tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgctatcat agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag
     2101 ggtttaaatg gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc
     2161 tagtacagaa caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga
     2221 gactatacga cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt
     2281 tttaggaact tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc
     2341 aactcaacta aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta
     2401 tcagcacaca aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat
     2461 agaaacatac ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga
     2521 tatgttgttt gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat
     2581 tactactttt tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg
     2641 acaattgtct ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt
     2701 aattggaact ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc
     2761 tgacgatagt cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt
     2821 ctctactgaa aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa
     2881 ttatgcattt aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc
     2941 gttgttagtg gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc
     3001 actatctaat ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg
     3061 tcttcgtacc ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt
     3121 agtaatgtcc agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa
     3181 atattctgag gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga
     3241 tcgtgaacca atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat
     3301 tccggatatt ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga
     3361 actctggacc ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc
     3421 tattaaacat actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac
     3481 taataaatgt gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga
     3541 tatttatatt cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa
     3601 aacttgggtt caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa
     3661 tgaaatgtct ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg
     3721 ttggcctact tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact
     3781 taaaaagaca tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact
     3841 tcctaaaatt gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca
     3901 atcatttaac cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt
     3961 agcagttctt ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt
     4021 tgattcttta aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac
     4081 actttcagga gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat
     4141 atggggtgat gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc
     4201 cctatattct agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa
     4261 ttttgtctct gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga
     4321 ttcgctttac cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat
     4381 catgactgat acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt
     4441 tcaatttagt ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa
     4501 cgctcactac ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta
     4561 tacccattgg gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc
     4621 aacagaatta acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat
     4681 aactgagtat ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat
     4741 atatgcacgg caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg
     4801 taatattttt tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga
     4861 gcagctcaat gcattaggat tagttactaa tgccatcata tattggaact cactgtattt
     4921 agaaaaagta tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg
     4981 caggcaatct ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata
     5041 cgaagctgaa ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg
     5101 ttcgggtttt gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat
     5161 agaaaaagag aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg
     5221 tgctaccgca ctatttcgtt tctatgttcc atgtacccca aaatcggtaa aagagaaggc
     5281 aatcaagaga actttattac gaatatcaaa aaattcattg ttcatttact ggaagctgta
     5341 cctaacgata tagaaaaact aaatttttct gattaccagg aacagaaaga aaaagaagca
     5401 gaaaaaagta ttgtaggaaa atgtcctaag tgtggcaaca atattgtatt aaaaaaatcg
     5461 ttttatggtt gttcaaatta tcctgaatgt aagtttactt tagctgaaca ttttagaaag
     5521 aaaaaactaa ccaaaacgaa tgtaaaagaa ttactggagg gaaaagaaac cctggtaaaa
     5581 ggaatcaaaa acaaagagaa aaagccctac aatgccgttg taaaaattgg ggaaaaggga
     5641 tatattgatt ttatatcttt ctcaaaataa acataaaagc cctttaaaga gggcttttat
     5701 atattaatca caaatcactt atcacaaatc acttatcaca aatcacaagt gatttgtgat
     5761 tgttgatgat aaaataagaa taagaagaaa tagaaagaag tgagtgattg tgggaaattt
     5821 aggcgcacaa aaagaaaaac gaaatgatac accaatcagt gcaaaaaaag atataatggg
     5881 agataagacg gttcgtgttc gtgctgactt gcaccatatc ataaaaatcg aaacagcaaa
     5941 gaatggcgga aacgtaaaag aagttatgga aataagactt agaagcaaac t
//
LOCUS       DACCSK010000137         5971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCSK010000137 DACCSK010000000
VERSION     DACCSK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.140"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            89..1024
                     /locus_tag="IVP39_002446"
     CDS             89..1024
                     /locus_tag="IVP39_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7587499.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1109..2119)
                     /gene="gap"
                     /locus_tag="IVP39_002447"
     CDS             complement(1109..2119)
                     /gene="gap"
                     /locus_tag="IVP39_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7587500.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            2407..3720
                     /gene="obgE"
                     /locus_tag="IVP39_002448"
     CDS             2407..3720
                     /gene="obgE"
                     /locus_tag="IVP39_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7587501.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            3941..4528
                     /locus_tag="IVP39_002449"
     CDS             3941..4528
                     /locus_tag="IVP39_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7587502.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            4705..5646
                     /locus_tag="IVP39_002450"
     CDS             4705..5646
                     /locus_tag="IVP39_002450"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7587503.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            5643..>5971
                     /locus_tag="IVP39_002451"
     CDS             5643..>5971
                     /locus_tag="IVP39_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7587504.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYD"
BASE COUNT         1928 a         1026 c         1376 g         1641 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacattggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt ttaaaagtag gggatgaaaa gaaagaagcc tttgactgga tccagtcaaa
     1081 ggcttctttt atcgattgat ctatcaaatt acaagttagc gaatttttct agtaagcgaa
     1141 tcatttggca agtaaatcca tactcattgt cataccatgc aacagtttta acaagttgga
     1201 aatcaccagc agttgttact tctgtttgcg ttgggtcaaa gatcgaacct tgagttgttc
     1261 caatgacatc gctagaaacg atttgacggt catcataccc gaatgaagga ttgtcaactg
     1321 tgtgtttttt gattgcttcg tttacttgat cagcagttac ttttgttttc aatactgaaa
     1381 ccaattcagt taatgaacca tcaacgactg gaacacgttg tgcgtgtcct tgtaattttc
     1441 cttgtagctc agggataact aagccgattg ctttcgcagc accagtagaa tgtgggatag
     1501 tattgtcagc agctgaacga gcagcacgca aattaccgcc ttttacaggt ccatctaaga
     1561 gcatttgtgt ggaagtgtag gcatgaactg ttgtcattgt accaacttcg atcccaaatt
     1621 cattatttaa gaaataagcc ataggtgcta aacagtttgt tgtacatgaa cctgctgaga
     1681 tgattttgtc gttttcatct aatgtatcat cattgacgtt atagacgatg gttttcatag
     1741 caccagctgg ggcagaaata actactcgtt tgacaccagc atctaaatgc gcttttgctt
     1801 tttcttcaga tgtataaaaa ccagtacatt ctagtacgat atctacaccg ttttcttttg
     1861 cccaagggat tttgcttgcg tcgggttctg cataaacacg agttgcttcg ccatccacta
     1921 cgattgagtc ttctgttgca gtaactgtgc caggataagt accatgagta gagtcaaatt
     1981 gcaataattg tgctaacata gttggacttg ttaagtcgtt gattgctact acttcaatat
     2041 catctgagac ttctttgatg cggcggaaag ccagacggcc aatacgccca aatccgttaa
     2101 tacctacttt tactgtcata tcaaaagttc ctcctttatt aggggtgctg tgaaacggca
     2161 tcctcaatta atacaattaa tacactgtta ttctgctacg caaagaatat tttgtcaaaa
     2221 aattatactt ggtagcagaa aaaaggttgg aaaccttacc aaatgataaa aaaagaagaa
     2281 ggtaaacaat ctttttgctg aaatgataga caaaatcaga tttcaccgat aaaataagta
     2341 agacttttgt ttattgtgga aattcaggca gtcaaataaa taaatgaata ataggaggac
     2401 ttaattatgt ccatgttttt agatcaagta acgatcgatg tgaaagctgg caagggcgga
     2461 gacggaatgg ttgcatttcg tcgcgaaaaa tacgttcctg acggcggacc ggctggcgga
     2521 gacggtggtc gtggcggcga tgtcatcttg atcgtcgatg aagggttacg tacattgatg
     2581 gattttcggt tcaatcgcca tttcaaagcg cagccaggtg aaaatgggat gagtaaaggg
     2641 atgcacggtc gtggttcaga agatacctat gttaaagtac cacaaggaac cactgtccga
     2701 gatgcggaga ctggtgctct tttaggagat ttaattgaaa atggtcaaac actagttgtg
     2761 gcaaaaggtg gacgcggtgg acgagggaat atccgttttg cctctcctcg gaatcctgca
     2821 ccggaaattg ctgaaaacgg tgaaccagga caagagcgaa aaatcgaact agaattgaaa
     2881 gtattggcag acgttggact tgtaggtttt ccatctgtcg ggaaatcgac tttactatcc
     2941 gttatttcat ctgctcgtcc aaaaatcggc gcttatcatt tcacgacgct cgtacctaat
     3001 ctaggaatgg ttactacaag tgatggcaga agttttgccg cagcagactt gccaggcttg
     3061 attgaaggag cttcgcaagg tgttgggctg ggtacacaat tccttcgtca tatcgaaaga
     3121 acacgagtaa ttttacatgt gatcgatatg agtgggatgg aaggacgaga tccttatgaa
     3181 gactatcttg ccatcaacaa agaattatct acttataact tacgcttgct agagcgtccg
     3241 caaatcatcg tggcaaacaa aatggatatg ccggatgcac cggaaaatct agtgaaattc
     3301 aaagaacagc tgaacaagga aaaagaagat gaatttgctg atgacatccc agtatttcca
     3361 atttctggag tgactcgaca aggattggac gcgttattga atgctacagc agatttgtta
     3421 gaagtaacgc cagaattccc attgtacgaa gaagaactgg aagaagaaac tgtacactat
     3481 ggattcaatc cagaaggtcc tgagttccaa atcgatcgtg attcagatgc aacgtggatc
     3541 ttatctggtg agaagatcga aaaattattc caaatgacga atttcgatca tgatgaaaca
     3601 gtgatgcgtt ttgctcgtca attacgtggt atgggtgtgg atgaagctct tcgggcacgc
     3661 ggtgcgaaag atggcgacct tgtacgtatc ggtgagtttg aatttgaatt tgtcgaatag
     3721 gcaagcaaat aaaaacaaat accaaaaaat atagattcag atttaaggga ggttgtgaca
     3781 gaagtgatca gcttcaagaa cgccgtttat tcggaaaaag gagctgtgat acgacttttg
     3841 tcacagccct attttattgg aaaaaaagag tgcttttctt ttcttatttt tcaattaaag
     3901 gtaaaataaa taacaagaag gtatgaaagg ggcgttttta atgagcaaac gagtaatcat
     3961 tttaaatttt caagtagaaa gccaggcata tcaggcattt tcagaaatca aaaagttaca
     4021 tgctgttcgt tcgattaaag gagaacagat ggcagttgtc agccatgatc ctaatgggac
     4081 acatcaattc aaaatagaag attttatcga ctttaccggt aataacaaat cttctacggg
     4141 tggattaatc ggaatgttgg ttggtgtatt aggtggtccg ctagggattt tattaggatg
     4201 gtttgccgga ggaatgatcg gtgcgacaca agatgccaaa gaaataaaag atgcccagac
     4261 cgtgttcgac tttgtaggaa ataaaatcgg tgaaggagct acaggactga tcttgattgc
     4321 tgatgaagag gacaatcgcc cactgaatca attggtaatg atggaattag gcggagaaat
     4381 tactcgcttg gattatgaag atgtcgaaaa agatgtgcat gatgcgaaag aagctgaaaa
     4441 tcatgcgaaa gaagctgcac aaaagacttg gaaggacaaa catccgcaaa cttccaacga
     4501 caatgaaagt tctggatctt caaattaata aaaaacagca gaggatctca aacgggttcg
     4561 ctgctgtttt tatagtaatt ttcaaagaaa acgctatcgc tttagaacgt tatttttaat
     4621 ttgaacccat tctcgactta cggttgatta tcccttcttt ttaccctaaa attaaagagg
     4681 aacttaaaga aagtcaggta gattatggaa ttacaatttt taggaacagg tgcaggtgtt
     4741 cctgcaaaac atagaaatgt gacaagtata gcattgaaac ttttagacga gcgcaatgaa
     4801 gtttggttgt tcgactgtgg cgaaggcacg caaatgcaga ttttgcgaac gactattcgt
     4861 ccgagaaaaa tcggtaaaat tttcattaca catttacacg gcgatcatat ttttggactt
     4921 cccgggctca tcagcagccg gtcatttcaa ggtggggata cgccattaga gatttacggg
     4981 ccaaaaggga tagaggaata cataaaagtc tctttaggta tttcgcaaac gcgtttaagt
     5041 tatccgctga aattcattga gctaaatgaa acagccccga tttttactga tcagcaattt
     5101 tctgtttata caaaaaaact aaatcatggg atcgacagtt ttggttatcg tgtggttgaa
     5161 catgatcata aaggagaatt gcaggtggat cgtctaaaag aattaggtat tccagctgga
     5221 ccactgtacg gcaaactgaa gcaaggtgaa acgatccaac tagaagatgg gcggacgatc
     5281 aatggaaaag atttcgtagg tccagataaa aaaggacgaa tcgtgactat tttaggcgat
     5341 acgagaaaaa cacataattc cgttgtgctt gcggaaaata gcgatattct ggtacatgaa
     5401 agcacattta ataaagatga agcaagaatg gcgcacaact atttccattc cacgactcat
     5461 caagctgctg aagttgcaaa agaagcgcag gcaaaacggc tgctacttac tcatatcagt
     5521 gcgagatatt taggaaaagc agctttagag cttgaaaaag aagcacaaga agtgtttgaa
     5581 aacacgacga ttatgaaaga ttttgatagc attgagattc catttcgcga ggaggaagag
     5641 gcatgaattt agaagataaa gttgttttag taacaggtag ttcaggcggt ctgggcgcac
     5701 aaatctgtta tgaagcagca aagcaaggag caatcgttat aagttgcgca cgaagaatgg
     5761 catttgtaga aggtgtcaga gacgagtgca gacgcttgag cggcaaagag gcttatgctt
     5821 tcaaggtaga cgtcagcaat cctgaaagcg tggacgaatt atatgaaaag gtgatggaag
     5881 aagtcgggcg cgtcgatatt ctggtaaaca atgcgggttt tgggattttc gaagacttcc
     5941 ttacattcga ccttggaaaa gcatacgata t
//
LOCUS       DACCSK010000138         5962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCSK010000138 DACCSK010000000
VERSION     DACCSK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.141"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IVP39_002452"
     CDS             complement(<1..450)
                     /locus_tag="IVP39_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7587505.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IVP39_002453"
     CDS             674..1465
                     /locus_tag="IVP39_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7587506.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSETANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IVP39_002454"
     CDS             1431..2384
                     /locus_tag="IVP39_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP7587507.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IVP39_002455"
     CDS             complement(2782..3756)
                     /locus_tag="IVP39_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7587508.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IVP39_002456"
     CDS             3871..4305
                     /locus_tag="IVP39_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7587509.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IVP39_002457"
     CDS             complement(4430..5416)
                     /locus_tag="IVP39_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP7587510.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..>5962
                     /locus_tag="IVP39_002458"
     CDS             5844..>5962
                     /locus_tag="IVP39_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP7587511.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIET"
BASE COUNT         1873 a         1125 c         1027 g         1937 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaaccgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag ac
//
LOCUS       DACCSK010000139         5940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCSK010000139 DACCSK010000000
VERSION     DACCSK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.142"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(93..536)
                     /locus_tag="IVP39_002459"
     CDS             complement(93..536)
                     /locus_tag="IVP39_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7587512.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(754..2094)
                     /locus_tag="IVP39_002460"
     CDS             complement(754..2094)
                     /locus_tag="IVP39_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7587513.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2363..3259
                     /locus_tag="IVP39_002461"
     CDS             2363..3259
                     /locus_tag="IVP39_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7587514.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3409..4350)
                     /locus_tag="IVP39_002462"
     CDS             complement(3409..4350)
                     /locus_tag="IVP39_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7587515.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4476..5597
                     /locus_tag="IVP39_002463"
     CDS             4476..5597
                     /locus_tag="IVP39_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7587516.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWKGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
BASE COUNT         1944 a         1124 c         1128 g         1744 t
ORIGIN      
        1 cttattcacg gcgttccaga agtagcttct tcttatttct tgaagctgat aacttctgga
       61 acatcctctt tttttatttg gacggataat ggtcattcca tttctttgat catttttttc
      121 attttgtcga tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca
      181 ggatctttta aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg
      241 gccttcatcc aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg
      301 gcaaaaacaa gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt
      361 ttccacatga acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat
      421 actcgtgtac gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca
      481 aacataacta ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa
      541 aacaaactcc tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa
      601 ttattttgca cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc
      661 ggaaaaagga gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat
      721 tccacataaa taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc
      781 attctttttt tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac
      841 cccaatggag ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa
      901 aaagctgatt cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc
      961 actgataatg atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa
     1021 taatttgtca ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa
     1081 taaaacagca cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc
     1141 ttttcgatcc ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat
     1201 agatgagaac aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt
     1261 cattttttca aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg
     1321 aaaaagaatc aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac
     1381 acctttgatt gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc
     1441 aatcagttca gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt
     1501 cataccatga ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc
     1561 tgtgcccaac atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac
     1621 actgatttta gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag
     1681 attgatgatc gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg
     1741 gaaccacaac tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca
     1801 catagaccag tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat
     1861 caaaccagat aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt
     1921 catcacgata tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc
     1981 gatcccaatc gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc
     2041 taacatggtg atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga
     2101 cctctttctt ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca
     2161 atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat
     2221 tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa
     2281 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
     2341 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
     2401 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
     2461 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
     2521 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
     2581 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
     2641 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
     2701 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
     2761 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
     2821 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
     2881 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
     2941 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
     3001 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
     3061 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
     3121 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
     3181 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
     3241 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     3301 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     3361 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     3421 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     3481 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     3541 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     3601 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     3661 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     3721 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     3781 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     3841 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     3901 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     3961 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     4021 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     4081 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     4141 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     4201 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     4261 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     4321 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     4381 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     4441 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     4501 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     4561 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     4621 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     4681 gtcagcgaaa aatcagctat ttattgacaa cttggaaagg aaaaaacacc gcagtcatcg
     4741 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     4801 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     4861 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     4921 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     4981 ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     5041 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     5101 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     5161 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     5221 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     5281 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     5341 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     5401 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     5461 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     5521 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     5581 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     5641 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     5701 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     5761 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     5821 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     5881 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
//
LOCUS       DACCSK010000140         5932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCSK010000140 DACCSK010000000
VERSION     DACCSK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.143"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            699..1433
                     /locus_tag="IVP39_002464"
     CDS             699..1433
                     /locus_tag="IVP39_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7587517.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1449..2618
                     /locus_tag="IVP39_002465"
     CDS             1449..2618
                     /locus_tag="IVP39_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7587518.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2634..3629
                     /gene="lacD"
                     /locus_tag="IVP39_002466"
     CDS             2634..3629
                     /gene="lacD"
                     /locus_tag="IVP39_002466"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7587519.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3708..4634
                     /locus_tag="IVP39_002467"
     CDS             3708..4634
                     /locus_tag="IVP39_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP7587520.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4653..5903
                     /locus_tag="IVP39_002468"
     CDS             4653..5903
                     /locus_tag="IVP39_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7587521.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1990 a          966 c         1210 g         1766 t
ORIGIN      
        1 tacggaaaga agttctgcac cttataaaat ttgtcaaaaa aactgttgcc attccaaagt
       61 ttgaaaaatg ctatttcgag gtagaaggat attatataag cacggttgac taaacgaagt
      121 gataataaaa aaagttattc aagaataaga aaaattatga tacgaattca gataaactgg
      181 agaatacaga gacctttaaa agggataaga aacaacataa attcctctta agagagaacg
      241 aaaggacaaa gccagcaact tgatacacga gctagtatta gaggaattta ttctcataga
      301 ctcccttttg aatacgtttc aactttaatt ttcatgttca taaatgattt gaaaccaaag
      361 caaatgcgtt gcacttttcc tccgtcttcc gaaaatcgtt caattcactg gtaagtagtc
      421 atttgatagt ttgaggcaaa ttgttgattg atgaagggta ggagctaaaa aaagatacca
      481 gttgaagaga gaaaaagaaa aaaatagagg gtgttcttga atgaaaaaag ttggaaaatt
      541 atttttcatt tccttttatt cctattaaag tcagtgatgt caatcatatt aacaagacta
      601 aaaatgaaaa taaaaaaaag attgttctta gtattgactc ataaaaaata ccactactat
      661 acttacattg tggttatagc cagttaggag agacatcgat gcaaaatacc tttcaatcaa
      721 aatcacttta ttatcagctg gtagaaatat tgaaacaacg aatcgaaaac agtatggtcg
      781 ctcatgacaa gctgccatca gaaagagagt tgaccagttt ttatggcgtt agtcgcacga
      841 cggtgcgact agcattgcaa gaactggaaa aaatgggcta catttatcga caacatggaa
      901 aaggaacctt tgtttcggaa ttaaggacaa atgcgactga tctagctggc gcatatagtt
      961 ttaccgaaca aatgcgggca atgggcaaac atcctgaaac caagatattg gccttcaaga
     1021 agattgaagc aactaaatat ttagcgagtc atctgaatgt tcaactaggt gagcctttat
     1081 ttcaattaga ccgattgcgc ttagctgatg atttgccaat gatgatcgag aaatcctatt
     1141 tgccagtgaa gctatttatg tccctttctc aagaaattct atttctgaaa cctttatata
     1201 atttgttttc agagaatttc aatcaaatcg tacggttggc tgacgaggaa ttttatgcca
     1261 gcattgtcca gaaagaagat agtgagttgc taaatattcc agaaggatca ccagtgctcc
     1321 atttagttcg ctcaacctat aatctcgaga acgaagtgat tgaatttaca ttaagtgtag
     1381 cgcgggctga taaatttcat tataagatca ggcatctgcg ttccactaac tgaattggag
     1441 gaaagaaaat gtttatgttc tcaaaagaag aactacgaaa gctaggagca gaaaatacta
     1501 cccgagaaat tcgacaacaa ccggaactgt ggcatgagac gtttgaccgt tatcaagaaa
     1561 cagttgcaga tttagatcgt ttgtttacgg atgcgaaaca acatgctgga gagcgaccaa
     1621 tccgagtcat ttttacaggg gccggaacct cgcaatatgt gggagataca ctaattccgt
     1681 ttcttactag tcaaggaaat acgaaccagt ttatttttca aagtattggt tcgaccgata
     1741 tcgttgctac gccaaaagaa tacctgattc ctgatgagac aacgatcttg gtctcatttg
     1801 cccgtagtgg aaatagtcca gaaagtctag ccgcagttaa gatggccaat cagctggtca
     1861 acaagatcta tcatatcgtg atcacctgtg caccagacgg attattggct acacaaatgc
     1921 ctacaaaaaa agaaaatcta gttttactaa tgccaaacaa agccaatgac gctggttttg
     1981 ctatgacaag cagtttcagc tgtatgacgc tgactgcgct acttattttt gatcaagcaa
     2041 gcaatcagca aaaagaaaga tatgttcatc aattaagccg actaggagaa gaagtcatcc
     2101 aacgagaatc agagatccaa gggtatctgg catcagactt tcatcgcatc atttacttag
     2161 gttcgggaag cttaggcgga ttgactagag aagcgcaatt aaaagttctc gagttaactg
     2221 ctggaaaaat cgcaaccgta tttgatacat ctttaggatt cagacatggt cccaaatctt
     2281 ttgtcaatga gcaaacattg gttttcctgt ttgtcagcaa tgactcatat acgcagaaat
     2341 acgatgtaga catgctggaa gaaatcaaaa atgacggtat tgcccaacgc gttgttggaa
     2401 ttggtcaaaa gggttcattg tttagtggtg attcctttga attagttgca gattcgttac
     2461 tgccggatgg ctatcttgct ttagcagata tcatggtagc ccaaacgatc gcattacttt
     2521 gttcggtgaa ggttggtaat acaccagata caccatcgcc aagtggaaca gtcaatcgtg
     2581 tggtcaaagg cgtaacgatt tatccatatg aaaaatagga aagagtgtga agcatgttag
     2641 aatggtcgga aaataaaaga caagcaatgg aacgtctatc gacgccagaa ggcattatta
     2701 ccgctttggc gatcgatcag cgaggcgcgc tgaagaaaat gatgtcggct ttagggatgg
     2761 aagcgacaaa ggaaaaaatt gagcagttta aaacactaat ttcccaagaa ttgactcctt
     2821 atgcctcagc aattttattg gatcctgaat acggactgcc tgctgcacag aagcgtgcag
     2881 cagcgagcgg tctgctgttg gcatatgaaa aaactggtta tgatcaaacc gtacccggcc
     2941 gcttgccgga tctgctgtcg gactgttcag ttctgcgatt gaaagagtta ggtgcggatg
     3001 cggtgaaatt tttactgtat tacgattatg aagaagcaga ggaaatcaat cggcaaaagc
     3061 aagtttttgt tgaacgaatt ggaagcgaat gcatggctga aggactgcca ttttatttag
     3121 agttattgtc atatgatgct aaaaatgtag atacgaaatc gctttcctat gccaaagcaa
     3181 aaccgcgcaa agtgatcgaa atgatgaaag aattctcgaa aacccagtat aaagtagatg
     3241 tcttaaaggt tgaagtacca gtaaacatgt catttgttga aggaaatgca acaggggaga
     3301 tcgcctatac acgtcaagaa gctgccgctt attttaaaga acaaagcgat gcaacagatt
     3361 tgccgtatat cttcttaagt gctggggtat cagcggcatt attcatggag acattgcgtt
     3421 ttgccaaaca tgctggggca cgattcaacg gtgtactctg tggcagagca acttggaaag
     3481 atgcagtcgc tcctttcgtt gaaaaaggag aagcggcaac tttagaatgg ctgacagaac
     3541 aagggacaca aaacattcgt caattgaatg aagtgataag agaaactgcc atcccatggt
     3601 atgagaaagt aagtgaaagc acttgttaat gtttctaata tattagtagt gataagagat
     3661 atgtgtgatt ataatttatc attattgaaa ctgggaggat attttatatg aataaaagat
     3721 tgttagatta ttcagcttca gattttttga aagctaagcc gatggaatta aagcaggcaa
     3781 ttttggcttc tgaaggtaga acaattatgg cagaaaatgt gccaagtagc tgtccttaca
     3841 tgggaaaatc agtgacaaat gcagaattgg agagagctgc tggagctgat ttaattttgt
     3901 ttaatgcact agatcttttt aatcctcaaa tttcaggtgt acctttaact ctaaaagaga
     3961 atccaataat ctggattaaa aaagctttag gaagagctat tggtgtaaat ttagaacctg
     4021 tagacaaaga agttttgatg agtgaaaatc gaactgaaat ttcaagtggt agaatcgttt
     4081 ctcctgagac tttaaaaaag gcaaatgaat taggctttaa ttttatttgt ttaactggaa
     4141 atccaggaac aggagtatct aatagaacta ttgagaaagc aataacatta tccaaatcaa
     4201 tttttaatgg attaattatc gctggaaaaa tgcatggtgc tggtgttgat gaaccaatta
     4261 tgaacttaga tatagcaaaa agatttatta aaacaggtgt agatatttta ttagttcctg
     4321 ctccatacac tgttccgtat ttcatggaaa gtgatttgcg aggaatcgtt gattatataa
     4381 aagattttaa tagaaataaa tctattgagg aaaaagttct agtactaacg gctaatggaa
     4441 caagccaaga ctcttctgac caagaaacta ttaaaaaaat tgcattagca tcaaaagcat
     4501 gtggagctga tattcaacac ataggggatt ctttaaatgg tatttgtctt ccagaaaata
     4561 tttttgcttt gggtcaagca attcggggat atcgtcatca aattattatg ttaggaaaat
     4621 ctaattatcg ttaattaagg aggaaatgaa agatgatcga caaagttcaa aaaatagctg
     4681 caaaaattag taatcaaaga catctactag ctttaagaga cagcatggct gcagttatgc
     4741 cattaatcat tattggttcc ttttttatgt tattagcaaa tttcccgata gaaggattta
     4801 caaaatggtt atctgaaatt ggagtacaat cctttttgaa caaagcatca gattcaacat
     4861 ttggtattat tggattagct gttacctact ctttgggaca caatctagca aagacttatg
     4921 gtgtagactc attatcagca ggattattat ctttgagtgg atatgtttta cttacacctt
     4981 tattggatgc agatggttct ataggatttc caacaaaata ctttggtacc tcgggtcttt
     5041 ttgttggtat tattgtagca cttgtatcta cagaaatttt ccggtggttt gtacttaaaa
     5101 atattacaat taaaatgccg gcaagcgttc caccaaatgt aagccgagct ttcgttgcta
     5161 ttattcctgg cttttttgtc gttctactat ggtttattgt tgttgtaatt tgttataaac
     5221 ttggaattga aaatgttcat gctcttattg cagatacact cgcaaaacca ctatctttac
     5281 tgacgaaaac tttaccagga attatattgg ttatacttat tcaatgtttc ttttggatgt
     5341 ttggtattca tggtgcacag gtaacggggc caataattga accgttatta ttgcaaaact
     5401 ctgatgtaaa tagaattgct tatcaagctg gaaaagaact tcctaacatt ataacttatg
     5461 aattcttata taactttgtt ttttctggtg gtgcaggttg tttatttgca cttgccattt
     5521 taacattttt cttttcaaag agtcaggaaa ataaaacttt aggtaagtta tcaatcgctc
     5581 cagtaagttt tcaagtagca gagccccttc tatttgggtt tccaactata ttaaatataa
     5641 aaatgttaat tccatttgta actgcaccag tagtaacaac attaattact tatttttcaa
     5701 tgagtatggg attagttgca aaacctgtag gagcaacaat cccatggaca actccaccga
     5761 taattgccgg atttttagct tctggtggac gaataagtgg tgcagtgatt caagtaataa
     5821 caattgcaat aaatgttttg atatactatc cattctttaa attagatgat aatgccaagc
     5881 taaaaagcga aaaaaatgat tagaactacc tatatgtttc taactgtagt ta
//
LOCUS       DACCSK010000141         5914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCSK010000141 DACCSK010000000
VERSION     DACCSK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.144"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(75..1169)
                     /locus_tag="IVP39_002469"
     CDS             complement(75..1169)
                     /locus_tag="IVP39_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7587522.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="IVP39_002470"
     CDS             1358..2119
                     /locus_tag="IVP39_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7587523.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="IVP39_002471"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="IVP39_002471"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7587524.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="IVP39_002472"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="IVP39_002472"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7587525.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4772)
                     /locus_tag="IVP39_002473"
     CDS             complement(3759..4772)
                     /locus_tag="IVP39_002473"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7587526.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4937..5857
                     /locus_tag="IVP39_002474"
     CDS             4937..5857
                     /locus_tag="IVP39_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7587527.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1805 a         1177 c         1062 g         1870 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcactaaaa agagcgtggg acaaaagtaa agaa
//
LOCUS       DACCSK010000142         5877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCSK010000142 DACCSK010000000
VERSION     DACCSK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.145"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(505..1314)
                     /locus_tag="IVP39_002475"
     CDS             complement(505..1314)
                     /locus_tag="IVP39_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7587528.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1314..2060)
                     /locus_tag="IVP39_002476"
     CDS             complement(1314..2060)
                     /locus_tag="IVP39_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7587529.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2078..2545)
                     /locus_tag="IVP39_002477"
     CDS             complement(2078..2545)
                     /locus_tag="IVP39_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7587530.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2545..2955)
                     /locus_tag="IVP39_002478"
     CDS             complement(2545..2955)
                     /locus_tag="IVP39_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7587531.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2966..3979)
                     /locus_tag="IVP39_002479"
     CDS             complement(2966..3979)
                     /locus_tag="IVP39_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7587532.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4196..4930
                     /locus_tag="IVP39_002480"
     CDS             4196..4930
                     /locus_tag="IVP39_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7587533.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVRNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4914..5612
                     /locus_tag="IVP39_002481"
     CDS             4914..5612
                     /locus_tag="IVP39_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7587534.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSHS"
BASE COUNT         1902 a         1232 c          978 g         1765 t
ORIGIN      
        1 cgcaaaacat cagcttaaag cgacaaaaac cacgagcagg tccattcatc ctcaaacctc
       61 ggtgatcttc gcggtttaac accatcagaa gaaatgcaca aaaataataa ggagagtctg
      121 agacataaat aaattcccag acttttagtg agtctaaatc cgaataaacg gtgggtcgaa
      181 agcaactcta gctgcgcgtc gatcgcagca atctctaggt gctatgttcg gataaatggt
      241 ggtcaaaagt cagattttcg gccatttcgt aacagccaac cagacaaaaa gcgtctgttt
      301 tgtcttctac tccaattgct caaatcttag cgacttttgg cttggtctta ctctaaacag
      361 tttgtaagcc taaactttat ttgctgtttg tttctaagat caaagcaaaa ctacttttta
      421 gttggttact tttacaagtt ctttttactg ttgaataaat gtaaatctta agaacaaata
      481 tttacttcta taattccaag tttcttaaaa aattccgcca agtgtaccta aaaagcataa
      541 aacaaaaatt cctagaataa ttgtgatcgt tttatgccct ttggagtaaa gccagacgca
      601 taaaaaggta attcctaatg gcagcaagcc cggaaaaatt tgatcaaaaa tactttgtaa
      661 tgaagtttca gctccaccaa ccgtgaattt tagtgacgta tttaaagtaa ccatcgtgcc
      721 tatcatcgcg ccaattaccg ttaaacctaa aatcgaagca ccatgagata atttgtcaat
      781 catatggttg tcttctgtat catcaataaa tttagttcct aaaacatagc catattttgt
      841 cgccaaccat ttaaaaataa aatgaatggc agtatacaaa gtaaaataaa ggatcatgcc
      901 taaaatattc ccttgttgag caaaagaaat acctatgcca gccgcaataa cccgaaaagt
      961 tccccagaaa aatgaatcgc caatacctga taaaggtccc attaatcctg ttttgatttt
     1021 attaattgcg tctgtatcaa attcaggatt tttagcattt tcttcttcca tggtgacgga
     1081 caagctcata acaaaaggcg caatatgcgg tgtaatgtta aatgtttccg tatggcgctg
     1141 caacgctgta taataatcat catcgttcgg atatatttta cgtaaggaac gttcaatacc
     1201 attcatttgt ccatatgcca tcattttatc gaaggaccaa gaggattgca taaaaaagga
     1261 gcgccaaaat atacttctta attctttttt cccaataaca ctttctttta ccattataaa
     1321 tcttcctcct cttcaaaagt tagcttttga acagccagta acgctaagca aagtccgacg
     1381 acagccacac cgacaacatc cattttcaga tacattacgc cgacaaatcc taagaaaaga
     1441 taaggcatca tcgctttatt cgccatcatt ttaattaata acgctagccc aacacaagaa
     1501 agcacaccgg ccgcaacatc aaagctgtcc aatacaacct gaggaatcaa cttgatcatc
     1561 gcattgattg tagacacacc aaaataagta gctacaaaaa ctaaaatggc cgatggaacc
     1621 ccaacgatta aaggttgagc cacataatga tatttttgtg cttttttgaa atcatgattg
     1681 actaatgcct catccatttt gttgttaaaa tacgtaattg gcattgcata ggcgaagtta
     1741 cgccacatct gaagaaagat ggacaaaggt aaagtcaacg caacggcagt tcctactcct
     1801 tgacccgatt gaatcgcaat tgctgtacct aaaacactgg aagcataaac ttcagggggc
     1861 gtcgcactcc ctaccataat tgagcccatg aacatggatt ccaaggtggc accaataata
     1921 attcctgact ctaaatctcc caaaatcaaa ccgacaaaag gcgctgtaaa tagcggtcta
     1981 cccatcatcg atgcaccaaa tgtgtattca tcaatagacg ccaaaaaagc taccagtgcg
     2041 attaatattg cttgtatcat attcttcccc tcctctttta gtcatgcaaa ggattgattt
     2101 ttttgtccgt tggtacagcc tgacattgaa ctgttatccc gttttcagtc atttttttca
     2161 ccaagtccag ttcttgttca ttcaagtaaa cggaactagt aatcattttc cgattctcgt
     2221 ttttctttaa atttcctaaa ttcacttctt taatctcagg tacgtgctga gtaatttcgt
     2281 aggcatcctc caccgtcccg acaattaaaa atacttggta tttcttgatt ttttctccat
     2341 ttaaaacaga gatggcctct gtcatatcct tcaccacaaa ttttgtccct gtaggaacag
     2401 ccatttttag tgtttttttg cgcagattat tggtagcgat ttcattactt gcaacgaaaa
     2461 tacaatttgc acctaagtta tttgtccagg caaaagcaac ttgcccatga acaaaacgtt
     2521 catctactcg taataattta atcattaaaa atcatcctct tctgttttct ctagcaactc
     2581 gttcaccaaa actatccctt cattcccgct attcatagct ttttggatta gttttttcgt
     2641 gtcctgttca cctgataagc aaagctctaa aagcagcggc agatttacac cgctgattaa
     2701 ataaaaattg tctttaccga tttgttcgga gaaagcgtta gacacgcttc ctcccagtaa
     2761 atctaccgtc acaattaatt cgtcatcagg cgctaagtcg gaaaatagct gctctatttc
     2821 ctggttaatg gagtagtcac tatctatata cgcagttatt gctaaaagtg atttttgagg
     2881 accaataata aattctaaag tatcctttaa gcctttagcc atcatggaat gcgtcgcaat
     2941 tacaattttc ctcatagctc ttaccttaat attccacagt ccgataataa cgacgaatat
     3001 ctagtgaatg atcccgcacc ttttcaaaat gagcactcac tcgttctaag gccgttgcca
     3061 taaccattgg cgctacatac tgacgcatag cgcttggtac acctgctaat tcgaaatccg
     3121 cagtgtcaaa aaccgtcaca acatcgctat atttatttac aaaacgctct acgcgatcga
     3181 ctagcggacg cgtttgatct tcacctttta ataacacaaa actcatagat ttatcagtca
     3241 tttcaatagt cccatggaaa aattcggctg catggatcga cttggtggca atccattgca
     3301 tttcttccag tacgcacata gcatattcat aggcagcccc ataagtattc ccagctccaa
     3361 caaacatatg aaattccgta tttttatgtt tttctgcaaa ttcaagtgct ttctgatcat
     3421 tttgttgccg aacacttagt aatacttccg gcattttttg taatgtttga ataaattcat
     3481 catattcagg aaactcaccg ttatttttca tcaggcgtgc acccaaagca aaaaactgta
     3541 actgaagttc ttctactaat gtatcgttgt cagcatcatt ttcaatcgca taatccacga
     3601 tttccccgag tggtgtagca gcatttccta caaacgcaat cgtcgttgca ccttgactat
     3661 tggcatactt aacaacatca acggtttctt gagtcgtacc ggttaatgaa gatgtgatta
     3721 acactgtatc tttattaatc tttttcggct gtgaacaaac cacctctgcg gctatttcat
     3781 gataataatc aagctctgaa tgagtgctta acagataagc tagcgggctg attgtggcat
     3841 atgttccacc agagcctacc aaataaagat ttttaaatcc cttctcactg attgcatctg
     3901 ccacctgatt gattgtctcg atatttttta ctgcaccatt taatactgat agaaaacgtt
     3961 cttcgctaaa acctaacata gttaaaaacc tccaagtcaa taatttgaaa aatatatttg
     4021 caaatgccgg cacattaaca aaccacttaa tcttttttat ataccacctg ttaacgtttt
     4081 taatgtacca cttatagaaa acgctgtcaa tagtttttaa ccacttttat ttttatttgt
     4141 accaaaatcc ttttttcatg tataataaac aaaaatagac tctggagtgt aacgtatgaa
     4201 tactgaagaa ctaacgaaca aattaataca agacattgaa caagaaaaat ataaatacgc
     4261 gcaagctatt ccttcagaac gtgattttgt tcaaatttat gaaatgaatc gttcagccgt
     4321 aaaaaaagcc attcaacaat tagtaaataa agagtaccta ctaaaaatac atggcaaagg
     4381 aacttttgtt aaaaaaaata tccatgaaca tttgcaaatc aaatttcatg gtttgtctga
     4441 attgttggaa cgggaaggga ttcagcctag cagcttaatt ttagcttcag aaataaaacc
     4501 ggctggacac attgtcggga aagctttaca gctggaacca actgaagaag tatttcattt
     4561 ggttcgttta agaagcggcg acggtgagcc aatttctatt gaagacactt atattccatt
     4621 aaatctcata cctgatcttc aagcttttga tttccaaatt ttttcatttt attccacatt
     4681 ggctgcttat cattatcaaa ttgaaaatat ctatcagaca atttccagct caaaagtgcg
     4741 caataaagaa gcccgtttgt taaaaaccag cttaaatcgt ccggtaatca gtcttgctat
     4801 cactgcggtt acgacaaatg aacaagcggt tgaatacact gaagtagtgg ttttgcctga
     4861 attcgccgcc atctatacta aaaatgttca tcaaggaaag gagatgaaaa tctatgccca
     4921 aattgattaa tctgttggct tatgacctga ttgacaaaat tattgcttat attcatgaac
     4981 atcatttaac ccccggaaca aaactgcctt cggaacggga cctatcagaa atgtttaatg
     5041 ttaatcgaac taccttaaga ggcgcgttac aaaaactggt ggacaacggc accttacggg
     5101 cgcaaaccaa tagcggctat tatttaactg cgccaaaatt cgaaaaaaat gcccatcatt
     5161 tctttacacc gatagaagat tctctgctta gtaagcagcg gctacagcta aaggcaacgg
     5221 ctctttcacc gaaaaaagca gcagctcttt gtccggaact gaatgatttc aaacagcagc
     5281 aaccacaagc ttatttagaa tatattgatg cagaagcaat tagcttgatg ttattaatcc
     5341 ctatggaaac attaaagaaa agtaatttct cggacaatcc tttccaagat tttaaaagtt
     5401 tggcatatac gcgttctcaa gaaatttctt taataactga caatgacgtt gcggcggact
     5461 tgctgggagt tgacaaagaa gaggttctgt tatttgtacg agaggaaata agagccggta
     5521 aggagttggc ggcgattgct ttaggttata ctattagtcg ccgctgcaac atacaggtta
     5581 atgttgctac gaccttgcaa tcccatagtt aggaaaaact attcgctgta agttcaaccg
     5641 tttcaatgca agcgaatcac aaatcaaaaa gtatttaact cgtgtttatt agaaatagtc
     5701 atgaactttt agtctccaag atcaaaccga cagcgttcaa ctctgagaag caaaagtaaa
     5761 tgtacgatca agtatgaggc tgggacagaa gcgctaagcg gttctgttgc aaagttttaa
     5821 atctactatc agataaggta gaataataga aaaagatagc aggaggaatg acgatga
//
LOCUS       DACCSK010000143         5836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCSK010000143 DACCSK010000000
VERSION     DACCSK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.146"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..335
                     /locus_tag="IVP39_002482"
     CDS             <1..335
                     /locus_tag="IVP39_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7587535.1"
                     /translation="EAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQMTEGKD
                     IWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTTRFGEF
                     IELHYVRK"
     gene            complement(414..968)
                     /locus_tag="IVP39_002483"
     CDS             complement(414..968)
                     /locus_tag="IVP39_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7587536.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            1221..1496
                     /gene="rpsP"
                     /locus_tag="IVP39_002484"
     CDS             1221..1496
                     /gene="rpsP"
                     /locus_tag="IVP39_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7587537.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            1508..1759
                     /locus_tag="IVP39_002485"
     CDS             1508..1759
                     /locus_tag="IVP39_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7587538.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            1884..2675
                     /locus_tag="IVP39_002486"
     CDS             1884..2675
                     /locus_tag="IVP39_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587539.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSLEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTKGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            2845..3366
                     /gene="rimM"
                     /locus_tag="IVP39_002487"
     CDS             2845..3366
                     /gene="rimM"
                     /locus_tag="IVP39_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7587540.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            3356..4117
                     /gene="trmD"
                     /locus_tag="IVP39_002488"
     CDS             3356..4117
                     /gene="trmD"
                     /locus_tag="IVP39_002488"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7587541.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            4253..4600
                     /gene="rplS"
                     /locus_tag="IVP39_002489"
     CDS             4253..4600
                     /gene="rplS"
                     /locus_tag="IVP39_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7587542.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            4859..5152
                     /locus_tag="IVP39_002490"
     CDS             4859..5152
                     /locus_tag="IVP39_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7587543.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(5240..5353)
                     /locus_tag="IVP39_002491"
     CDS             complement(5240..5353)
                     /locus_tag="IVP39_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAP7587544.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
BASE COUNT         2031 a         1026 c         1122 g         1657 t
ORIGIN      
        1 ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cactcacgca caattacaat
       61 tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga
      121 cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac
      181 atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg gacgggattc
      241 ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg acacgattcg
      301 gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa aaaaggcaac
      361 tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc tttttactct
      421 gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa acttaatgtt
      481 ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat atatgcttcc
      541 ggttgctgca aggttggcac attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg
      601 ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt atgatcccaa
      661 acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac tgctggcaca
      721 gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac ttcctcacga
      781 aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt gtaaaatggc
      841 agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt cgcaaaagct
      901 tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa tccaattttt
      961 tttgtcatac gatttttcct ccttcaaact atctataata atcttacaaa aaataagtaa
     1021 ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt atttattttt
     1081 aacaaaaaag aagtctaaaa tcttctattt cctaaaaaaa atcgaattta atcaagctgt
     1141 aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac
     1201 aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa
     1261 agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca
     1321 tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag
     1381 acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc
     1441 tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag aaatagggtg
     1501 accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta
     1561 tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac
     1621 agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat
     1681 ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat
     1741 cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg
     1801 ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta
     1861 taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat
     1921 tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta
     1981 tatctgaaac tgttaccagt ctagaaaaaa gtagtaccaa aacggcttcc accactgaac
     2041 aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac
     2101 agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag
     2161 cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt
     2221 tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt
     2281 ttgcttctag tcaagaaaca atccaagttt tgcagccctt tgagatcgac acaaagggtc
     2341 agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt
     2401 ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc
     2461 aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag
     2521 cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag
     2581 aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg
     2641 cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat
     2701 catttaactc caagtcacag gaagtaatca aaaatagcac gccacatttt tgcataattt
     2761 tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag
     2821 acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac
     2881 acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt
     2941 tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca
     3001 gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct
     3061 atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta
     3121 gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat
     3181 ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc
     3241 gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa
     3301 gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa
     3361 gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat
     3421 cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc
     3481 agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct
     3541 caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa
     3601 aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga
     3661 attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg
     3721 tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga
     3781 attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa
     3841 tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac
     3901 tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa
     3961 attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga
     4021 tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa
     4081 agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca
     4141 gtaggttgat aatatgttaa tattttatgg tgagtgcaaa agcacttaac tattacgata
     4201 ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc
     4261 attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg
     4321 tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt
     4381 tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa
     4441 agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa
     4501 aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt
     4561 acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcattgttat cagactgaga
     4621 aagaaccctt ggtattcaag ggttcttttt tcttagcatg taatttgaac accctgatcc
     4681 agtttttttc tttaattatt ggtaggggag aataatggat tagaataaat ctacctataa
     4741 gtagtagtaa aaaaaagagc actatgataa tatctttctt aagagtaacc gctttaataa
     4801 aaaatccaga atattctaac ataaatggag tattcttaga atcaaaagga gaaagaagat
     4861 ggtttatact tatacggagc aagaaaaaat gaaagtttta gacacattca tgaatgatgg
     4921 aaaagtaaaa caagtaccaa gttcaacaaa aaagaaatac atcctattga aagaagtctt
     4981 gaaacgcttt gaccatggag tgacttatac agaaacagag gttaattcga tactgctgaa
     5041 cgtattttct agtggcgatt atgtcgaaca aagacgttat ctaatcacat ttggcttttt
     5101 caaaagaagt tcagacggtc gggcatatca gatgatggga attgaaaatt agaagtttta
     5161 atttatactc attgaaaaaa gttcaagtat gcgatattgg ttctacaccg catacttgaa
     5221 ctttttttgt catctgtatc tataatacaa agccaagaac gttattcaaa atatggatag
     5281 ctatgataaa agctaatgag cgattcgcag ttaaataagc aatcgtcagt aaaatcgttc
     5341 caggagcgta catgaaaaaa tcaattggat gcgttacatg aataaagata attctctttt
     5401 atttgtacga ataaatacca aaacgtacct cctaaaatta gattttggac agatgacctt
     5461 taaaaagata acaagatttc gttcttgaaa caatatatat gcttttattc caatgaggtt
     5521 gtgaaaacag tattttgtcc tgagcgttag aaaattgaaa atggggaata gcaactcgta
     5581 ctttaccatt ttttaagtga atacaagatc agctaattga acaccgttta ttcgtgatga
     5641 gggacacaac actgcagtcg acacatttct cttttatgtt caaagataac aaatatttaa
     5701 aacaaagaaa aatagtagat aatcccgtcc taactctttt attcttccct cctagcttcc
     5761 aaatttaagc aatacttcaa ttaatcaagt tttatctttt tttttcaata atcgtaaaaa
     5821 aagagtattt tttatt
//
LOCUS       DACCSK010000144         5808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCSK010000144 DACCSK010000000
VERSION     DACCSK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.147"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            27..833
                     /locus_tag="IVP39_002492"
     CDS             27..833
                     /locus_tag="IVP39_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587545.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            856..1085
                     /locus_tag="IVP39_002493"
                     /pseudo
     CDS             856..1085
                     /locus_tag="IVP39_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1244..2179
                     /locus_tag="IVP39_002494"
     CDS             1244..2179
                     /locus_tag="IVP39_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP7587546.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            2223..2492
                     /locus_tag="IVP39_002495"
     CDS             2223..2492
                     /locus_tag="IVP39_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587547.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            2768..3043
                     /locus_tag="IVP39_002496"
     CDS             2768..3043
                     /locus_tag="IVP39_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587548.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            3085..3507
                     /locus_tag="IVP39_002497"
     CDS             3085..3507
                     /locus_tag="IVP39_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587549.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            3527..5119
                     /locus_tag="IVP39_002498"
     CDS             3527..5119
                     /locus_tag="IVP39_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587550.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            5140..5475
                     /locus_tag="IVP39_002499"
     CDS             5140..5475
                     /locus_tag="IVP39_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587551.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            5621..>5808
                     /locus_tag="IVP39_002500"
     CDS             5621..>5808
                     /locus_tag="IVP39_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587552.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2261 a          827 c         1166 g         1554 t
ORIGIN      
        1 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagcaaga caacgtttta
       61 aaaaacgaaa aagttatctt agatggctgg ggatctttat gattctcggc tttttgattc
      121 ttgctagtcc gatttacaca cataaaaagg tagacatacc agatgaagta agcacgaacg
      181 catttttaaa cacgaaggaa ctgacgatgg tttcaaaaga aaaaggagcg aaccagcaat
      241 tagtttttga atttgcgatc gacgatgaag atcagagctt attaaaagaa ctagctaatc
      301 ttaattatcg tgtagaagta aagacagcta aaggggatta tcaagcaata aaagccaaag
      361 tgatcaaggt gtctgatgat tattttgtag tgaagttagc acatgtacca gaagaatata
      421 ttgctatgcg attaactatt attcctgaaa aaattgatcc aaaggtggat atgcaagaac
      481 cgcaagatct gattttttat attcacgaag ataaggtcaa agatcacgtg aaagacgaag
      541 attatgaaca acatgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
      601 cacaaaaaca aattgaatcc ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
      661 aactaaaaga tcaattgcct tatcaagtcg cagaagatca ggaaaatact gaaaataaga
      721 ttaatagtta ccagcaagaa attgaaacat cgaaaacgca aatgaaagat caagaagaga
      781 gcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaatatt tagaagaata
      841 ggtggagtaa aagaaatgaa gcgagcgaac ttggtcggca aagatacttt cgctgagcag
      901 aaagtattga ttaaagccta tctcaaagtt gtaaaagcta aagaaatatg tcaagaagtg
      961 aattatctaa cacaacaata tctgagttga agaatggtag aaagagttta gataccttaa
     1021 aattcagatc atttgaagct ctatatatag ctgctaaaaa atattttaca gctgataata
     1081 aataaaaaat taaggtgggt tctatgtgaa aaaaacgtat caaaagtttt tatggattag
     1141 ctatatcgtc ctatttatcg ttgggtttct cgtcagtgtg tccgtgatcg tcggaacgct
     1201 ttttcttttg ttctctgacg aagatctggt acttaagggg ttaatgagta cgttatcgtg
     1261 tgtcatgtta ctttatttat gtatacagat caacgaaatt atgccccctc attggcaaga
     1321 aaagaagaaa gaatttaagg aagaaatcga aataaaatcc aatgactgga ttgaatacac
     1381 atggcttcct ctgaaaagaa atagcaaacg aaaggaattt tgggcattgc aattatggcc
     1441 aataggaaaa ctttttattt atatggcttc acttatggtt actttaatcg gttatttggt
     1501 ggtggtctat ggggtggcct attacttgtt aagtgagcca aaacagatcg cggcgtcaaa
     1561 tattttcacg gcagtaatga cattaatgtg tgtcatgtgc ttttctattt ttagttactt
     1621 actcgtccaa tttatcaggc aaaatttaac aatgagtaaa cttttcaaaa gtttattgga
     1681 cttacttatc ttaggcagta tgttaacgac tctaacagat cggttcgaac aatctgaaaa
     1741 aacggtagag gggataaaga acttcttaac gacgattgat caatttataa atggatatat
     1801 gtatttgttt gaagtaggct ttattctcac tttgtttggt tgtgtattta taaaactcat
     1861 taaaagatca atcgaaaaag aaatggttat tgatctaatt ccgacgctga gcggctatga
     1921 gtattacgtt gattttggaa agtatgagga gttgatccat acggatatcg ttcacacgag
     1981 tatccatgaa gtaaccgtta agggcatcag atctacttta aaagatggac aggagacgaa
     2041 aacaatcgac cgaagaaaag aggtctacaa tttttataaa gttgaagcaa gtctacgata
     2101 tggcattggg aaattgaggt tagatgggag taatattcaa caaaacaaac catatgtttt
     2161 aggaaaagtg gtgatttaga acttcctatg aaagatgaat aaataaggag aatcttttga
     2221 aaatgattct tctttacaga attgtgctta tagttggcac ggagttaaaa atcttaaaga
     2281 aggagcagtt attcaaagtg aaagaacatc ataatcagtt actatgtatt aaaacgggta
     2341 cgatgaacat tcaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     2401 cgattcgaga gcaacataag caagaaaaat taatggctac ttatagccaa ttgattgatg
     2461 agtgggaaca attcaaggaa tctaaaacat aaaattccat ttaaacacct tattttttaa
     2521 agagcataag gaaacgtgta cccactttca aaataaagtg ggtagatagg ctgtgcctat
     2581 cacgtttctg aaaaaaataa atgaccccaa aattttcaat ttatgtgtag gtttatgggt
     2641 agcaaaaata tgaatgtggg cagcaaaaag ttcatttgtg ggtagtaaaa ttcaaatgtg
     2701 tgtagcaatg tgtgtagcta cacacattgc tacacacgct ataacatata gtgaaaagga
     2761 gatatttatg gaagtaaaaa taagagatat tgatgaaaaa acgattcaaa gaattgaccg
     2821 aagatgtatt gaattaagtg aacgcaccgg gactaaatgg agccgaaatc gatacctaaa
     2881 agttttgatt gaaaatgatt ttgaccatga acttatggat catagaaaag atcaatttga
     2941 tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaattt tagaagaata
     3001 tattgcaaca aataatcaat taatcgcctt gttaattgaa taataaaaat agtattgtga
     3061 atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt atatctttac
     3121 gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat atctaattat
     3181 agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga aaaaataaat
     3241 ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctatattaac cgaaatggtt
     3301 tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac aaattcaact
     3361 gtctatcaag aagcaaaaga gcgtgtggaa ggcaagataa aagcgaatca aacgaaaaag
     3421 tattctccta agatcaagaa accaataaat gaatctgttc atgccagagt agacccgttt
     3481 gatccaacaa agtttttaaa aagataaaaa gtagaagggg aacatgatga ataaatcgcc
     3541 tgcagtcatt ctcacttgtc aatttacctt gcccaataaa aaatcttttt caacatacat
     3601 tgattatatg acaagagaaa aagcgctgga agaaaaggaa cgacgcacgc cggacgaaga
     3661 acaggaactg caacgaatca agaatgcttt agaggatttt tatattcctg aaggagaaac
     3721 tttttcggag agtaagacag ataaagaatt cagtgaaacg tcgaatgaag ctcaatcgtt
     3781 gatggaacat ggaatcaatt ttgacaagtt agaggaacaa gattttacca aatatctttc
     3841 ttatatgacc cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa gggaacaaaa
     3901 agaataccaa cggttgaacc aagcaatgaa aaaatatcaa acagagccac ataaaaaagc
     3961 ttcaggaaat cttcctggag ttttttctat ggcttcagac gaagtgaaag gaagtgatct
     4021 gaaggagatc aaacaaatct tccaaagagg gcaacaaaaa ggctcaatca tttatcaaga
     4081 tgttgtctct cacgacaatt cgtatcttga aaaaatgggc ttgtacgatc ccaaaacaga
     4141 taccttagat gaagaaggat taaaagcagc tggaagaaaa atgatggaga cattattcga
     4201 gaaagaacaa ttgaatgaaa caggttactg gatggcaacg attcaccgta acactaagca
     4261 tatccatatt cattttgcag ttgttgaaaa agaaaatacg cgtaaacctt acacggaggt
     4321 tgaaaatggt gtgagttata tcgtaccgaa aggtaagaga agacaagcaa cattagacgc
     4381 aatgaagacc aattatgtac atgagttaga acgtttttct tatgaaaagg atcgaatgat
     4441 tggtatggag aaacgaaatt tattgacgcg gaaaagtgat ttgcgtaaca ccttaacaaa
     4501 agtggtaaaa gagccagcgc gttacgatcg acacgcaatg aacttattaa atgaagtata
     4561 taaaacccta cctgacaaac gttcggaatg gcattatggg gatgaaaaac gaaccaaact
     4621 ttcaccatac acaagagaga aactcgatga gttaaccaaa tatgtgttaa caaaagaacc
     4681 tgaatatcaa gaatatatac agatttcaaa atcattgaaa gaagaagcaa aaagtcttta
     4741 tggggaaagc aagagagaat caaaggatgc agaaaaaaat gctctctttg atttgaagaa
     4801 gcgcacagga aatgccattc taacagaact gaagaaacag gatagtcaaa taaaaccttt
     4861 aatagaaagt atggatcaga tgcaatctat ttacaatgaa cgatcctttc gaacgcttaa
     4921 acagttacca acgaatgaat acaaatatag ttacgaagaa ttcatgaagc gcaggaaaca
     4981 attccaacaa agagttgtca cgcaacggag tttgcgtcaa ttaaatcgaa cgattaatgc
     5041 ccatgaagaa aaataccacg caagtaaagc ctacgaagag caacaacgaa gaattcaaat
     5101 ggaacaagaa agacagtaaa gacagtagga ggaatagcaa tgaaacaata tttagatcaa
     5161 tggaaagtta tagaaggatc attaagagaa gaacgaatcg aacaacttcc cgattgttta
     5221 gaaaaagagc atttgtttca gataagagaa atgttgagaa acgaacagtt tgatccaaat
     5281 caatttttag tcgttgagta ctcagctact ggtgtgtact gttgtaatca tgtgaagggt
     5341 gaaaaatatt ttatcattca agaatatgag ggcaagttag cgccttatta tactacttgg
     5401 gaaatgaatg aagaggggat caataacttc ccgtgtaaaa gcattgaaga gtctatttca
     5461 ctcacagaat gttagttgtt aagtgaataa aagaagtaaa agagtcctga acgtaattgt
     5521 ccaggactct ttttttcaat gaattgtttt ccgccaattc aatgagtggt gcgatttgcg
     5581 actacattat atcaaaagaa agagaaggga ggaagaagaa atggcttatt caacgattgg
     5641 agttgttgtt gaaaaaagca gagacaatct cgtttttgtg acagaaattc aaacgggcag
     5701 agcttttgtc gtcacagaca aagcggcaaa agcgtatcaa agtggcgata ttttaacgtt
     5761 aaatctgact acgaagacgt tcgtcgatgc ggcagaagat tatccatt
//
LOCUS       DACCSK010000145         5789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCSK010000145 DACCSK010000000
VERSION     DACCSK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.148"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(145..543)
                     /locus_tag="IVP39_002501"
     CDS             complement(145..543)
                     /locus_tag="IVP39_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7587553.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(540..1313)
                     /locus_tag="IVP39_002502"
     CDS             complement(540..1313)
                     /locus_tag="IVP39_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7587554.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMVKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1310..1993)
                     /locus_tag="IVP39_002503"
     CDS             complement(1310..1993)
                     /locus_tag="IVP39_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7587555.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2160..3047)
                     /locus_tag="IVP39_002504"
     CDS             complement(2160..3047)
                     /locus_tag="IVP39_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7587556.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKITIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYDA
                     AAAITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3044..3913)
                     /locus_tag="IVP39_002505"
     CDS             complement(3044..3913)
                     /locus_tag="IVP39_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7587557.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4050..5336)
                     /locus_tag="IVP39_002506"
     CDS             complement(4050..5336)
                     /locus_tag="IVP39_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7587558.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTGWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5609..>5789
                     /locus_tag="IVP39_002507"
     CDS             5609..>5789
                     /locus_tag="IVP39_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7587559.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMP"
BASE COUNT         1797 a         1270 c          967 g         1755 t
ORIGIN      
        1 ttgaaaagaa agtctgatgc ttagagtaga atttcataaa aatttactat ttcataagta
       61 ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct catccgcctt cttgtttgtg
      121 tttatttcct cttcaaacgc ttgtctatga ttcagacttt tctctatcgt tattttcatc
      181 taaaagttct tgcattccga taatttctcg atattgatga atcttagtga taccaatgac
      241 tttgtctaca tcaatactaa aacaaatacc attacctgga atatcgatgt tgatgccttg
      301 accaatcgca tccattactt tattcgtcaa actatcgggt actaatgtca acacaatatc
      361 tttttctggc gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac cagaacctct
      421 tccatgtaag atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga taacatcttc
      481 gcccattcct ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta caagatcttt
      541 catttgcttt cccccaattt ttgcaatctt tctttttcga ttctgtcttt ttcatctttt
      601 cgtttataaa tatatcctag tactaaagtg gataatattg gtgtcaatgc aatcattgca
      661 accacaccaa acccgtcaat gatcggatct cttccttcaa tttgtgcagc gattgccacg
      721 ctcattgata aaataaatgt cgaacacatc gggcccgttg ctactccacc gttatcaaaa
      781 gccattgcta aaaagatctt gtccactttt cgtcctaaaa taaatgctag cccgtatcct
      841 gggatcaaga aataataaag agagaatccg acgagcaaac gccacataga caatcctact
      901 gctgcgccga tcccaattga aattaccatc agcatgattt tcgatcggat tctcccctcg
      961 ctcaattctt caacctgttt gaccattaca tgaatcgccg gctcagcaaa ccctactaag
     1021 aaccccatta aaaagccaag gggaaccagt atataattgt aaggcaatcc cgcaatagca
     1081 ctgcctaaat gctgtcctac cggtacataa gccatattga ctccgtgcag gaaaaggatc
     1141 aacccaatag tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt tttgcgcata
     1201 cgaaaactaa tgatattcat taaaacaaat aggattacga taggtgccac tgccaaaatg
     1261 acttccatgg taacgatttc gattccatct atcacttgct gccagaaact cattggaaga
     1321 tcacccccaa aatcatcacg gcaagaatcg gccctaacga agcaataccg accattccaa
     1381 aactgtcatt ttcatttttc ttttgtttga tcatactagt catccctccg gcaagggcca
     1441 agataaatgg aacagttact ggaccagtag tcactccccc cgaatcaaaa gcaatcggca
     1501 taaattcgga actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct atagccatca
     1561 attgataata ggatagcttg aatacgaccc gaagcagcgc aaaggctaga aatactccag
     1621 tgccgacgct gacgatccca atcagcaaca ctctgccaat ctctccgtca gatatatcat
     1681 atacttgttg tcccaaaact tgcacggaag gttcagcaat cgtgatcacg atcccgatag
     1741 caaatcccaa actgaccaaa acagtcaaac ttttcttttt gatcatatat ttccctacta
     1801 gatctccgac ttccatcata gaataatctg ctccaaacaa aaagagcgtc attccgatca
     1861 tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc tccttctaac ggtgcaaaaa
     1921 caaacgttaa aatgacaatc aggacagtca tcggcaatat agcagcaatc acttctttaa
     1981 aattttttag catgtatcct cctcctttac tctttaagtt taccttaaaa tgaaagagtt
     2041 ttcgagcaca aacttaaaac gttatcattt aatttttctc aacataaaat aacacacgtc
     2101 ccaaaagtat ctttttactt ggaaaaatga ctttgaagga acgcgcttat tgtgtttttt
     2161 tattttacag agccgatcat tccttgaacg aattgctttt gcaaaacaaa aaatacgatg
     2221 gcagttggta aagtagcgat gactaatcca gccatgatca ttccatagtc tggtgtatag
     2281 gaagcaccca tagcagataa aacaagcgga accgtccgtt tatcaggaga ttgcaaagct
     2341 actaagggcc ataaataatt gttccagcta ctcatgaatg tgatggctgc agccgcgtca
     2401 taggtatttt tagccgtcgg catatacact ctaaaaaaaa tacttagttc ttttagaccg
     2461 tctaatcgag cggcttctac tagatctttt ggaaaagatt taacattttg tcgaaagaag
     2521 aagatcaaaa aagcagtgct aacagatggc aggatgacaa ccaagaaact gttgattccc
     2581 aaaaccgttc cgttcaattg tgaaaacatt cgatataaag ggatcatcaa tgccgcaaaa
     2641 ggaaccatca tcgatagcag tagaatattg aaaattaggt cttttcgttt actttgataa
     2701 atctcaaatc catatcctgc cattgacgaa atcagcaatg caaacacagt ggtcaccaaa
     2761 gcgatcatcg ctgaattcca aagggaacga gtaaaattca gctcattgga aaacaaattt
     2821 tgaaaattga tgatcaagtt actgccaatc gttatttttc ccgatgtgat atccacgggt
     2881 gtattcgtca ttcccaaaac catccagaca aaagggaaaa tggaaataaa tgataggatg
     2941 cttaatgctg tatactttaa tactgaccca attgtcgaac gaagttctaa tgcttgtttt
     3001 ttgacgttgg ggcgttcgat tgtttctttt tctaccatat ttttcatcga ctttctcctc
     3061 ctactttcgt ttggattatc gacaatatca caataaagga aacaataaca aaggaaactg
     3121 ctgccgcata tccaaaatta ggcgtgaatt tataactcaa attatagatg tattgagata
     3181 gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt ttgtacttca tcaaaaagct
     3241 gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat gattggtttt aaatttggga
     3301 tcgtcacttt gataaactgc tgccatttag aagcaccgtc aatagatgct gcttcataaa
     3361 tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat catattgtat ccagtccaac
     3421 gccaagtgat cgaaagaatg atcagaactc tcgcccaaat tggatgagtc aaccagccaa
     3481 tcggtgtgtc aatcatacca atgcccaaca acaagttgtt tacaaggcca gattgagaaa
     3541 atagactttt catgatcaga gaataactga ccatcgaagt gatacatgga agaaagatag
     3601 cggttcgaaa caacccttta tatttcaact ttttatcatt taaaaggttg gaaataaaaa
     3661 gtgctagaaa caacatgatc ggtacttgta caagtaaata caggaatgta ttaaaaaaag
     3721 cttttttaaa agttgcatct gtcaacatcc gctgataatt acctaacccg ttaaatgtca
     3781 tattgacagg gggaccactt tgaaaagacg tgatcaaagc caaaaccatc ggaataaaaa
     3841 ccatgattga tatcaaaatg actggcaaga ttaaaaaagc atgcccgttt ttgaataaat
     3901 ctttctgctt cattcttttc cctccaattc tatcattgat tatctacatg aaaagacaag
     3961 aaagacaata tctgtaaggc agacgtgatc ttttttgtct tttcgtttgt ctgatgaaaa
     4021 agcgaagaaa ttcacttttt taccaccgat caattagcca attgtgcttc tacttgtttt
     4081 tgtgtatctg ctagtacctt gtctgtctct tctccattga tgattcgatg aagagcttca
     4141 gctacgactg cttcaattgc ataggtctca tgtccgtaat tcacttctgg tatttcagat
     4201 gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt aaaattcaga agcttcttga
     4261 taagcttctt gttcattcgc gctaagcatc gtagatacta gtccgatttc tttcgcaaga
     4321 gtgcccatca gttcttcatt cgaagcaaac gttgcttcta ggaaatcttt cgctgaatct
     4381 tctccagcta ctcctttgat cacataccag ccagcgccgc caaggttaga agcttgcgct
     4441 ttttgcagat tttctggaag tgcaggtatt ggtgcaatct tccattttcc agattggttt
     4501 tctgctcctt ggatcgttga agaataccaa gcgccagttg ggctagaagc tactgctcct
     4561 gattgtatcg cagttactcc agcattccag tcagaggttt gttcgactag atcttctttc
     4621 aaaagagtgg caaacaattc aagaccatac tttaaagatg cattgtcttg gatcgtgact
     4681 gtctccccat cttcagatgt ataccattct cctgcctgtt gcataatcat ccttactcga
     4741 ccaaggtcag aaggattgac ttcagcgatt ttctttcccg tcttttcttt gacatctcgc
     4801 gctacttgaa tatactcgtc ccacttcaga ttctccatat cctcttctgt ataacctgct
     4861 tctgccatca gatccgtacg atagaagttt gctgttacgc ccgaatcgaa aggaacaccg
     4921 tatattttat ctcctacttt gtttaccgca aatttatacg gtgcaaaatg atcttccttt
     4981 acaatagcag acaaattagc gaatgcatct ggataagaag tcaaatagcc ttgtatccta
     5041 taatcttcaa tcaagacgat attgggcaat ccttctgtat tcccgctcgc taaagcggtg
     5101 ttcagtttct gaacgatatc atcctgagac atcgtaacaa cttctatctc tgtttcttcg
     5161 ttctcataaa ctttttttgc ttcattcact gctttgatat tgaatgtctc atcccacgcc
     5221 cagccagtga ctttttcttc tttgtctgct gtactactag cacttccgca tcctgttaag
     5281 aaacctgatg ctgccgctaa acaaacgact ccagcaatta actttctgat gttcattttt
     5341 atccgctcct tttccatcca ttagttgttt acgtttgcta cattcatcat accaaaattt
     5401 gaaaacggtt tctttattat tcagatactt caaaaatttc tcctaaaata ataaaaaaat
     5461 acaaatcaac tatgacaaaa ggaataatct tagcatctag accaaagatc gaaatggtga
     5521 ggtttcttta aaataaaaat atccttgcat tttctcctag aaataagtat ttttaaatta
     5581 caagactatt cttaaggcgg gtaaaaaaat gccaatacgc gtaattctta tcgatgatga
     5641 acagacgatt ttagatggaa tgaaacagtt gatcaattgg tctgagcacg ggtttcaatt
     5701 aatagggact ttttccagtc cgttgaaagc gcttgatttt tctttggtac atgcaatcga
     5761 tatcgtggtc acagatgtaa tgatgcctg
//
LOCUS       DACCSK010000146         5357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCSK010000146 DACCSK010000000
VERSION     DACCSK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.149"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1616
                     /gene="hflB"
                     /locus_tag="IVP39_002508"
     CDS             <1..1616
                     /gene="hflB"
                     /locus_tag="IVP39_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7587560.1"
                     /translation="VMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDP
                     RRFVELGARIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASR
                     VRDLFETAKKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEG
                     VIVIAATNRSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKV
                     VAQQTPGFAGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKK
                     EREMVAYHEAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFE
                     QIVGLLGGRTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQV
                     FVGRDYGQTKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFET
                     LDAKAIKSLFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEA
                     KHELNEENKAEHDSDSQQSEDNHSDDQYK"
     gene            1833..2720
                     /gene="hslO"
                     /locus_tag="IVP39_002509"
     CDS             1833..2720
                     /gene="hslO"
                     /locus_tag="IVP39_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7587561.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            2721..3725
                     /gene="dusB"
                     /locus_tag="IVP39_002510"
     CDS             2721..3725
                     /gene="dusB"
                     /locus_tag="IVP39_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7587562.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            3836..5332
                     /gene="lysS"
                     /locus_tag="IVP39_002511"
     CDS             3836..5332
                     /gene="lysS"
                     /locus_tag="IVP39_002511"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7587563.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT         1849 a          948 c         1243 g         1317 t
ORIGIN      
        1 gtgtcatgaa ctttggtaaa tcaaaagcca aagaagcaga caaaaaagct aatcgcgtac
       61 gtttttctga tgtagcgggt gcggaagaag aaaaacaaga actagttgaa gttgttgaat
      121 ttctgaaaga tcctcgccga tttgttgaat taggtgcccg tatcccggca ggtgtacttt
      181 tagaaggacc tccaggaaca ggtaaaacct tattagcaaa agctgttgcc ggagaagctg
      241 gagtaccgtt ttactcgatc tcgggttctg atttcgtaga aatgtttgtc ggtgttggag
      301 caagtcgtgt tcgtgatttg tttgaaacag cgaagaaaaa cgcaccagct atcattttca
      361 ttgatgaaat tgatgcggta ggtcgtcaac gtggtgccgg tatgggcggc ggacatgatg
      421 aacgcgaaca aacattgaac caattgcttg tagaaatgga tggcttcgac ggaaatgaag
      481 gagtaatcgt cattgctgca accaaccgtt ctgatgtact agacccagcg ttactacgtc
      541 caggccgttt tgaccgccag attttagttg gacgtccaga tgtaaaaggt cgtgaagcaa
      601 tcttgcgagt ccatgcacgg aataaaccga tggcagatga cgttgacttg aaagttgtgg
      661 ctcaacagac accaggtttt gccggagcag atttagaaaa tgtcttgaat gaagcagcgt
      721 tagtagctgc tagaagaaat aaaaagaaaa tcgatgcttc tgatatcgat gaagcggaag
      781 atcgcgtgat tgcaggtcca gctaaaaaag atcgcgtaat cagcaaaaaa gaacgcgaaa
      841 tggttgctta tcacgaagct ggtcatacga ttgtcggatt agtattgagt cgcgctcggg
      901 tcgtccacaa agtaacgatc atccctcgcg gacgcgccgg cggatatatg atcgctttac
      961 caaaagaaga tcagttcttg atgacgaaag aagatatgtt tgaacaaatc gtaggcttac
     1021 ttggtggacg tacagctgaa gaaatcatct tcaatgtcca atcaactggt gcttccaatg
     1081 actttgagca agcgacagct ctagcacgaa gcatggtaac agaatatgga atgagtgatc
     1141 gtttaggtcc tgttcaatat gaaggaaacc atcaagtgtt tgttggccgc gactatggtc
     1201 aaacaaaagc ttactcggaa caagtcgctt tcgagattga tcaagaagta cgaaaaatct
     1261 tgatggaagc tcatgataaa gctcgagaaa tcatcgaagc tcatcgtgca cagcataaac
     1321 tgattgctga aaaacttttg gaattcgaaa cattggatgc caaagcaatc aagtcattgt
     1381 ttgaacacgg tgtgatgcct gaaggtactg agtcaaatga ttatccaagc gaaaaagaag
     1441 cgaaaacttt tgaagaagca aaacgtgctt tggaagaaaa agacgctgaa aaacaagcgg
     1501 aagaaaaacg tgattttgaa gaagcaaaac atgaactgaa tgaggaaaat aaagcagaac
     1561 atgattcaga ttctcaacaa tcagaagata atcattcaga cgatcaatac aaataaaaaa
     1621 atataggtca attgatctga tatgactccc gaaaaccgga acaaaaaccg gttttcggga
     1681 gatttcttta tttaagttgt aaatagggtg atgtcctgtt ttctagatga ttctgaatta
     1741 tttttaaaag aagcattcca aagttgaaaa aagcagtagt tgctagtatg attcactatg
     1801 agtttaaaaa cggaaaaagg agtacataac aaatgaaaga ttatttggtt aaggcacttg
     1861 ctcacgacgg attcgtccga gcatacgccg ttcaagcaac aaataccgta gcggaggcac
     1921 aaagacgtca tgatacttgg cacacctcct cagctgcttt gggacggaca ttggtcggta
     1981 gtctgctact tggtgcgacg ctcaaaggag aagacaaact aactgttcga atccaaggcg
     2041 atggtccagc aacaggaatt gtagtagatg caaatggaaa gggagatgtg aaaggttaca
     2101 tcaaaaatcc gcatctcagt cttcctctaa atagtgaagg taaaatcgat gtgcggggag
     2161 cagttggaac acaaggaatg tttactgtca caaaagactt aggattaaaa gaaccttttt
     2221 ctgggcaaac gccgattgtt tctggagaaa tcgctgaaga ttttacttac tatcttgcta
     2281 tctcagaaca aatcccttca gctgttggac tgagtgtatt ggtcaatact gacgacagca
     2341 tcaagacggc gggaggattc atgatccaaa tcatgccagg tgccgatgaa gaaacaatca
     2401 gcaagatcga agaacaatta aaaacgacac caagaatctc taccctctta gatgaaggac
     2461 agacaccaga agaaatattg aaaggattgc ttggtacaga agaattgaac attttggaaa
     2521 aaatgcctgt tcaattcaaa tgtgactgtt caaaagaaaa atttgcagaa gcaattatcg
     2581 cgttaggtgc agaagaaatc caagcaatga tagacgaaga tcacggtgca gaagcggttt
     2641 gttctttctg taataacaaa tacaagtata gcgaagaaga attagatgga ttgaagaaag
     2701 aagccttagg agggatttaa gtggaacaca catggaaaat tggcaatgtg gaaatcccta
     2761 atcgtgtcgt tgttgcaccg atggctggaa taacaaattc agcatttcga gtcactgtca
     2821 aagaatttgg tgcgggctta gtcgtatgtg aaatgatcag tgaccaaggt attcatttca
     2881 gaaacaaaaa aactttagag atgctccaca ttgaagaaac agaacatcca ttaagtttac
     2941 agatatttgg cggaaataaa gatagtctag ttgaagctgc tcaatttgtc gaagaaaaca
     3001 cagctgccga tataatagat atcaatatgg gctgtccagt aaataaagta atcaaagcag
     3061 aagcaggagc aaaatggctt ttagatcctg agaaagtata cgaaatggtc caagctgttt
     3121 cttcagcagt aaatatccca gtaacaatca agatgcgtat tggttgggat gaagagcacg
     3181 tctttgctgt agaaaatgca ttagcagcac aatcagctgg agcaagtgcc atcgctatgc
     3241 atggacgtac acgggttcag atgtatgaag gaaaagcgga ctgggaagta ttgaaagaag
     3301 tgaaaaaaca tttgacgatc ccatttatgg ggaacgggga tgtcaaaacg ccagaagatg
     3361 caaaatggat gcttgactat gtcggtgcag acggtgtgat gattggtcgt gcagcgctgg
     3421 gcaatccatg gatgattcac cgcacacagc attatctcga aacaggagaa ctgattcctg
     3481 aaccaacacc agccgaaaaa attgctacag ctaaacttca tttacaacgc ttagcagatc
     3541 tcaaaggaga aaaagtagct tgtagagaat tcagaacaca tgcggcttat tatttaaaag
     3601 ggattcctcg agcagcgaaa gtgaaagtag caatcaatca gacagaaaaa cagcaagaaa
     3661 tcaatgacct gctggatcaa tttgtagaaa aagcggaaaa agtactattg aaacaaacgt
     3721 tgtgatttct aagtttttcg tgaattttca agccgaaatt ccaagttcgg cttgcttttt
     3781 ttgtgtttca ttggcattat aggaaagaga ttaacatgag aatggaggaa cttttgtgac
     3841 acaggaacaa caacatcaag tagaagattt aaatgatcaa atgcttgtac gtcgtcaaaa
     3901 aatggaacaa ttgcgtgaag atggaatcga tccatttgga aaacgatttg aacgcacgca
     3961 caactcagaa gaattgcatg aactatttga tccaagaaca aaagaagaac tagcagaaat
     4021 gggattgacc gctagtgtag ctggtcgtat gatgactcgc cgaggaaaag ggaaaactgg
     4081 atttgcgcat ctacaagatc gtgaaggcca aattcaaatt tatgtaagaa aagaccaagt
     4141 aggtgatgag gcctatgaag tattcaaaca tgcagacctt ggagatttct ttggtgtaac
     4201 tggacaaatc atgaaaacag acacaggcga agtttctatc aaagcttcag aaatcacgat
     4261 tttgtcaaaa gcacttcgtc cactgccaga taaataccat ggcttaacga atattgaaca
     4321 acgctacaga caacgctact tggatttaat cagtaatcgc gaaagctttg accgatttat
     4381 gaaacgaagc caaattatca gcgaaatccg tcgctattta gatggcaatg gctatattga
     4441 agtagaaaca ccagtattgc acaatgaggc aggtggagct gccgcacgcc catttatcac
     4501 gcatcataat gcactggaca ttgatttata tttgcgtatt gcgctagaac ttcatttaaa
     4561 acgactaatt gttggcggaa tggaaaaagt ttatgaaatc ggccgcgttt tccgtaatga
     4621 aggaattgat actacacata atccagaatt tacgatgtta gaagcataca cagcatatac
     4681 tgatttcaat gatgtaatga atctgacaga aggtattatc cgtaacgcag ctgaaaaagt
     4741 actaggaaca gcaaaaatca catatgatgg acaagctgtc gacttagaaa gtgactttaa
     4801 acgcatccat atggtagatg caatcaaaga acaaactggt gtagacttct ggcaagaaat
     4861 gacattggaa gaagcattag ctttagccga aaaacacaat gtagaagtaa ccgaagctat
     4921 gggtgtcgga catgttatca atgaattctt tgaaacattt gttgaagaaa cattgacaca
     4981 accgacgttt gtatacggac atccagtcga agtttcgcct ttagcgaaga aaaatccaga
     5041 agatcctcga ttcacagatc gattcgaact attcatagtg gggcgcgaat ttgctaatgc
     5101 ctttacagaa ctgaacgatc caattgatca aagagaaaga ttcgaagcgc aagaaaaaga
     5161 acgtgaacta ggaaatgacg aagctcacgg tgtagacgaa gacttcttgg aagccttaga
     5221 atacggtatg ccgccaactg gtggtctagg catcgggatc gacagattag taatgttgct
     5281 aactgatgca caatcaattc gggatgtttt gctattccca acaatgagat aatcgaataa
     5341 aagctgtcta aggaatt
//
LOCUS       DACCSK010000147         5354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCSK010000147 DACCSK010000000
VERSION     DACCSK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.150"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1004
                     /locus_tag="IVP39_002512"
     CDS             <1..1004
                     /locus_tag="IVP39_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7587564.1"
                     /translation="TGVPLQQLEKKESERLLELEGLLHERVVGQDEAVKAVSRAIRRA
                     RSGLKDPDRPIGSFMFLGPTGVGKTELAKALSEVMFGSEDALIRVDMSEFMEKYSTSR
                     LIGSPPGYVGYDEGGQLTEKIRQKPYSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSK
                     GRKVDFRNTIMIMTSNIGATQIREEKNVGFNVQDVTKDHKAMQKRILEELKKAFRPEF
                     LNRIDETVVFHSLSKDEIHTIVQIMSKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPE
                     YGARPIRRALQKEIEDRLSEALLGGEIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            1225..2148
                     /locus_tag="IVP39_002513"
     CDS             1225..2148
                     /locus_tag="IVP39_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7587565.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            2166..3407
                     /locus_tag="IVP39_002514"
     CDS             2166..3407
                     /locus_tag="IVP39_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7587566.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            3541..4887
                     /gene="gor"
                     /locus_tag="IVP39_002515"
     CDS             3541..4887
                     /gene="gor"
                     /locus_tag="IVP39_002515"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7587567.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1830 a          862 c         1176 g         1486 t
ORIGIN      
        1 ggactggtgt ccctctacag cagttggaga aaaaagaaag cgaacgcttg ttagaactgg
       61 aaggacttct gcatgaacgt gtcgttggtc aagatgaggc tgtcaaagca gtttcacgtg
      121 cgatccgccg tgcacgaagc ggattgaaag atcctgatcg tccaattggt tcattcatgt
      181 tccttggacc aactggtgtc ggaaaaacag aattggccaa agcactttca gaagtcatgt
      241 ttggaagtga agatgccctg atccgtgttg atatgtcaga atttatggaa aaatacagta
      301 caagccgatt aatcgggtct cctccaggat acgtagggta tgatgaaggt ggacagttga
      361 cagaaaaaat tcgtcaaaaa ccatattcag tgattctctt agatgaagtg gagaaagcac
      421 atccagacgt tttcaattta cttctgcaag tattggatga tggtcattta acagattcta
      481 aaggtagaaa agtggacttt agaaatacaa tcatgattat gacttcaaat atcggagcga
      541 cacaaattcg agaagaaaaa aatgtcggat tcaatgtcca agatgttact aaagatcaca
      601 aagccatgca aaaacgtatc ttagaagagt taaagaaagc tttccgacct gaattcttga
      661 atcgaatcga tgagactgtc gtcttccatt cattgagtaa agatgaaatc catacaatcg
      721 tccaaatcat gagcaaagcg attatcaaac gattgaaaga acaagatatt caagtgaaaa
      781 tcacaccatc tgcaatcgat gtgatcggga aagctggatt tgatcctgaa tatggtgcac
      841 gtccaatccg tcgcgcactt caaaaagaaa ttgaagatcg attgagcgag gctttgcttg
      901 gcggagaaat tcgtttaggt gaccacgtaa cagtaggtgc ttcaaaaggt aagatcacat
      961 tgaatgttcg cggacctaaa aaggaaacag taggttccct atagttactt ggcctgtgta
     1021 gctgaatttt tataatagta agacttgatt aagaggttgt gacaagattt ttgtcacaat
     1081 ctctttttat atttggacag aatatatcga actttatttt gattttttgt gaaataattt
     1141 tcaaaaatcc gactttaaat agaagaagta ttggaaagtt tttagagcta tgctactatt
     1201 atgaagttgt aggaggagaa aattatgatc gaactcataa ccacagtaga aactattgaa
     1261 caagcagaac agttgcttgc tgcaggtgca gatacactat atttcggtga agagcaattt
     1321 ggattgcgtc tgcctgcttc atttacacga gaagaacagc gacaattggt agaattagca
     1381 cacaagtatg gtaaaaaagc gaatattgct gtaaatggga tcattcatcc tgaaaaaatg
     1441 aaaaatgtac ctgaatattt ggctttttta aaagaaataa acgtagatgt gatcacagtt
     1501 ggtgatacag gtattattta tacgatgaaa aaaaatcctg aattgcgtat accgtatatt
     1561 tatgatgctg aaacattagt aacgagcgca cgtcaaatca atttctgggc gaaaaaaggc
     1621 gcagttggag cagtacttgc tcgtgaagtt ccttttgaag agatgaaggc gatggaagaa
     1681 aaactcgata ttcctgtaga aacccttgtg tatggtgcta catgcatcca tcaatccaaa
     1741 cgtccactgc tacagaatta ctataactat acgaaacaag acgaacaaaa agatcgtgaa
     1801 agaggtcttt ttatctcgga acctaaaaaa gaagagacac attattctat ttatgaagat
     1861 agtcatggca cacatatttt tgccagcaat gatctgaatc tctcgaatga attgaatgaa
     1921 ctttatcagc atcattactg tacatggaaa ttggacggat tatttactcc aggcgaaaaa
     1981 ttcgtaaaca tcactcgact attcgccgaa gcaaaaaaac aaattttaga aggtagctgg
     2041 gatcaagaaa aagcggaaaa aatgactagc gaaatcagta aattgcatcc tgaaaaccgt
     2101 gaattagata caggattctt ttatattgat ccttctgcta tcaaataagg agtatgatga
     2161 aagaaatgac agtaaaaaga aaattgaaac gaccagaaat cctagcgcca gcaggaacac
     2221 tggaaaagtt aaaaaccgcg atccattatg gagcagatgc agtatatatt ggcgggaatg
     2281 catatggttt aagaagccgt gcaggtaatt ttacttatga agaaatggct gaaggagttg
     2341 cttttgcaaa agaacatgat gcgaaggtat acgttgctgc taatatggtc acccatgaag
     2401 gaaatgaaga tggagcaggg gaatttttcc gcagtttgcg tgatgtcggt atttctgcag
     2461 tgattgtttc tgatccagct ttgatcgaaa tttgtgcgac agaagcccca ggtctgccga
     2521 tccatttatc cactcaagct tcagcaacta attttgagac attggaattc tggaaagaag
     2581 aagggttgga acgtgtcgtt ttagctcgtg aagtttcaat ggaggaagtg gcagagattc
     2641 gtaaaaatac ggataccgaa atcgaagcat ttatccatgg tgccatgtgt atctcttatt
     2701 ctggtcgatg cacattgtcc aaccatatgt cgatgcggga tgcaaaccgt ggcggatgtt
     2761 ctcaatcttg tcgctggaag tatggattgt tcgatatgcc ttttggtcaa gaacgaaaca
     2821 gtttaactga tacaggcgaa gtggaagaag aattctcgat gagtgcagtg gatatgtcaa
     2881 tgatcgaaca tattccagaa ttgattgaaa acggcgtaga cagcttcaag atcgaaggaa
     2941 gaatgaaatc gatccattat gtatctacgg tgtcaaatgt ctataaagcg gctgtagaca
     3001 gttatatgga agatcctgaa aactatgtct gcaagcaaga atggatcgac gagttatgga
     3061 aagtagcaca acgagaatta gctacaggtt tttactacaa tacaccaact gaaaacgaac
     3121 aattattcgg tgaacgtcgt aaaatcccag tatataaatt tgtcggtgaa gtattatctt
     3181 atgatgaggc aacacagatt gctacgatac gtcagagaaa cttgttttct gtaggagatg
     3241 aaatcgaatt ttatggtcca ggattcagtc atttcagtca aaccgttgaa gttatgcata
     3301 atgaagaagg cgaatccatt gaccgagcac caaatccaat gatgatttta actatgccag
     3361 tcgcacagcc ggtgaaacct ggagatatga tccgaaaacg taaataagga aaaagctgat
     3421 atatccctgt tttaagcatc ttcgcataca aaattcaaga aaagtgttac acttgttttc
     3481 tgtagaaaaa ggatcgttaa ataaaggttt tgattgatct cttcaataag gaggaacaaa
     3541 atgcaaatat atgattacat cgtaattggt ggcggctctg gcggtattgc ttcagcaaac
     3601 agagcaggga tgcatggcgc aaaagttttg ctgattgaag cgaatgaatt gggcggaaca
     3661 tgtgtcaacg tcggttgtgt acctaaaaaa gttatgtggc aagcaagtac gattttggaa
     3721 acaattgaac gagatgcctc aagttacggt atccaagcag atttaaaaaa tgttgatttc
     3781 gccggtctag tagaaagacg agaaaaatat attgattttc tacacggtgc ttatcaaaga
     3841 ggattagata gcaatcatgt cgaggctata aaaggttacg ctacttttat cgatagtcaa
     3901 acgatcgaag taaacggtga aacctatcgt gctcctaaaa tcttgatcgc tactggaggc
     3961 cgtgcaagta aaatgaccat tcctggtgga gaatacgcaa ttgattcaaa tggatttttt
     4021 gaattaacag agttacctaa gagagcaatc gttttaggtg ctggatacat tgcggctgaa
     4081 ctttcaggtg tatttcgtgg tttaggtagt gaagtcatgt gggcatatcg aaaagaacga
     4141 ccacttagaa cgtttgataa aatgctttcg gacaatttga tccaaatgta ccaagaagct
     4201 ggaataaaaa cttacgcaca tcatattgct aaagaaatta ctaaaaacaa tgacgaatat
     4261 acggtgctat ttgaaaatgg tgaaacctta actggtgatt gtgtactttt tgccggtgga
     4321 cgtgtaccga ataccgaaaa attaggtctg gaaaatacaa atgtggaatt ggacaaaaaa
     4381 gggtttatca aagtagataa ataccaaaat acaacggatg aacatatttt tgctgttggt
     4441 gatgtgatcg gtaaattaga tctgactcct gttgcaatcg cagctggtag acgtttatca
     4501 gaacgtttat ttaatggaaa gaaagattct tatcttgact ataaacttgt tccgacagtc
     4561 gtctttacgc atccaccaat tgcaacaatt ggtttgacag aagaagaagc attggaaaaa
     4621 tatggcgaaa atgaattgaa agtttatcgt tcacgcttta ccccaatgta ctttgcatta
     4681 aatgattatc gccaaaaatg cgaaatgaaa ttgatctgtg tcggaaaaga agaaagaatc
     4741 gtcggcttac atgctattgg tgttggtgtc gatgagatgc tgcaaggttt tgccgtggct
     4801 atcaagatgg gtgcaactaa agaagacttt gacaatacag tagccatcca tccaacagga
     4861 gcggaagaat tcgtaacgat gcgataattt aatattaaca gttccaacag ctttttattg
     4921 aacaaaccat gtaaaaccag aaaatcagta tactgatttt ctggtttttt gttttttagt
     4981 tatttatgta agattgcttc gataattctc aaaacgaaat aagtagggaa gttggagatt
     5041 ttgattatct tcgtaactga cgaagagtaa atgaatccat ttggtatgca ctcttaagtt
     5101 caaaatattg gtatcgatgt ttaaaagaaa tagaaatgta tcgaagaaca gatgtttaaa
     5161 aaagtgtgac tttgtataaa taaattagaa gaagcaaacg aggatgttaa aagaatacca
     5221 tatagttacc aaaccaagaa agttctcgtc aagttcgctg gtacgaatca aatcaagtga
     5281 aaaaagggac tgtgaaaaaa attgtgtcat agttagtcac tttttccgaa caaatagtgt
     5341 tcaatcagct gtcc
//
LOCUS       DACCSK010000148         5127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCSK010000148 DACCSK010000000
VERSION     DACCSK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.151"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(27..530)
                     /locus_tag="IVP39_002516"
     CDS             complement(27..530)
                     /locus_tag="IVP39_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7587568.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(653..1417)
                     /locus_tag="IVP39_002517"
     CDS             complement(653..1417)
                     /locus_tag="IVP39_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7587569.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMMLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1524..1799
                     /locus_tag="IVP39_002518"
     CDS             1524..1799
                     /locus_tag="IVP39_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7587570.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2238..3188
                     /gene="yidC"
                     /locus_tag="IVP39_002519"
     CDS             2238..3188
                     /gene="yidC"
                     /locus_tag="IVP39_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7587571.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKKNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQQKNSTNNKGRNAGKQQKRK"
     gene            3310..4519
                     /locus_tag="IVP39_002520"
                     /pseudo
     CDS             3310..4519
                     /locus_tag="IVP39_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(4887..5108)
                     /locus_tag="IVP39_002521"
     CDS             complement(4887..5108)
                     /locus_tag="IVP39_002521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7587572.1"
                     /translation="MFKNYTINQTILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
BASE COUNT         1597 a         1008 c          940 g         1582 t
ORIGIN      
        1 atatagtcta ttattggttt tacctattaa atcgaggatt ctttcccaaa gtaacgttgc
       61 catctcttac gcatacttgc agacatgggc tgagccgctt ctttgactgc gcagtatttg
      121 tccacgaatg tcacgatata tccttctttg tacttaggcg gtgcaatcgt tgcaccccac
      181 atgtgcttca aaatgatatc tcgctcaaga tccgacaaat cggtgatttt ttctgcattc
      241 tttacagcaa ttcttggatg gatatatgca tgtgtcccct cgtcaaattt cgtcgtacgc
      301 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
      361 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
      421 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
      481 tcttgtacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
      541 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
      601 ggattattct agtcgctcta gataaaatag ctaaagctta atagttcctt aatcataaag
      661 aaaaatgata aagaacgatc cctgctgcga ctccgacatt caatgattcc gcatttcctt
      721 tcattgggat atatacgttc tgatcagtca aagacaagat ttcctgactt actccctgac
      781 cttcgtttcc catgattata gcgaaatcct ctgttttttc tactttgtct aatgcatgtg
      841 cttcttcatt taattctgta ccgtacaccg taaacccttt gcctttcatt acagggatca
      901 cttctgccaa atctttttgt aacacaggca aatgataatt gcttccctgc atgcttcgca
      961 gcacctttgt actataaata tctgccgttc ctttacctaa taaaacacca gccataccaa
     1021 acgcatctgc cgtacggatc atcgttccca catttccagg atcctgtaca ttatctagta
     1081 aaagccagcc tcctgaaaaa tctggcgtat ctttagatgt taacatcatc actgctaaga
     1141 tcccttgtgg tgtaggtaga tctgacatag cggtcatgac ctcatctgaa actagaaaag
     1201 tagaaatctc tttgtttaca gccagccagt ctccccattc tttcatacct ctctgtgtca
     1261 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcgatcaga tggaaccctt
     1321 ccaaaagata ttctgccttt tcttctctgt atttcttttt atgtagtttt tttaattctt
     1381 tgattcttgg attttttgct gattggatct ctttcacatt cctcacctca cctgattata
     1441 tcatggaata ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat tgatttataa
     1501 aacaggaaag aaaggtgacg aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc
     1561 agggcgtggg atttcgctat atgaccaaga tggttgctga tcagttaggc gtgaccggaa
     1621 cggtaaaaaa cgaagacgac ggctcagtca caatcgaagc aatcggaaat gacgacatca
     1681 tccaaaaatt tatcgaagaa gtcaagaagt ctccgagccc aagtggacga gtgcagtatg
     1741 tggatgtcca agaagatccg atgattgagg aacgaaagaa atttgatgtg gtgggataag
     1801 gttgtgaaaa agctggtctg catcaagcat taaggactgg ttgtgaaaaa tagctctcca
     1861 tatttttcaa caggctggac ttaatgccgt agatgcaagc ttttgaacac cgtcgataag
     1921 gttgtttttg aggcgctttg cctcgagtat taaaaacagg tagtaaaaaa gctgatctat
     1981 aacttgcaat aaatttgctt aaccctcatt ttccagacag aacaaaaaag tttttagttt
     2041 tttaaataag aaagaaacac ttggaatcac tcttttcttt ccaagtattt ctttcttgat
     2101 tttttgtctt ttttcaaaat ttttcgctac gaaaaagaaa gaatatttat atgaaatttc
     2161 tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat
     2221 agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca
     2281 gtatcctgct ctttttatct ggctgcgtgc ggatggattc aaacggaaac cctgacactt
     2341 caggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg
     2401 agaatttcaa ttggtcttac ggctgggcag ttattgcaat gactgtgatc gttcgtatca
     2461 tcatcttgcc actaggtatc agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc
     2521 aagcgttaaa accacaagtt gaagcagcac aacaaaagct aaaagctgca acaacacgtg
     2581 aagaacaaat ggcagcacaa gcagaaatgc agcaagtata tcgcgaaaac ggattgagta
     2641 tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt
     2701 attttacggc acgctacaca gaaggtatcc gggaatcatc tttttacggc atcgatctag
     2761 gctcaccaag tatggtgctt gtggccatcg caggggtcgc ttatctgcta caaggatata
     2821 tttcaacaat cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct
     2881 ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca ctttactggg
     2941 tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg atgaaaccac
     3001 gtttgaaagc acaagtggca gaagagttga aaaaaaaccc gccaaaacaa gtcgtcactc
     3061 caagaaaaga cgtgacagaa tcagcaaagc cagctgacaa caaacaagca acaaaacaaa
     3121 tcaaacaaca aaaaaacagt acaaacaaca aaggccgcaa cgcaggaaag cagcagaagc
     3181 ggaaataaaa atatatgaca aaaaactgta gacaaataaa aaaatttctt tgttctacag
     3241 tttttttgtt acaattaaag aaaacgttcg cttttcgagg aggaatgtat aatgatgtcc
     3301 aaataatcaa tgtatagtcg aatggaagaa gacaaaattt cgctagatgc acttgttcct
     3361 aaaaatcatt tagtccgtaa aattgacaaa ttattgtcat ttgattttat ttatctcatt
     3421 gttgaatcaa cttactcgac cattggtcgc cctagcgttg atcctgtcgt cttagtaaaa
     3481 ttagtattca ttcaatattt atttggaatt tgttcgatgc gtcagaccat taaataagtt
     3541 gaaacaaacc tagcttatcg ttggtttctt gggcttagtt taattgataa agttcctcat
     3601 ttttcaactt ttggtaaaaa ctatgcacgg cgtttcagag aaaccgattt gtttgaacag
     3661 atatttatca ttattctcga ataagcagtt gatgctagat ttattcatga agaaattttg
     3721 tatatggatt caactcatat taaagcgaat gcgaacaaac gtatttttac aagagatatg
     3781 gcacataaag aagccatttt ataccaaaaa caattggaag aagaaatcaa tgaggcaaga
     3841 attggcgaag gcaaacgccc ttttggttca atcattcgtc aggaggtggt cgaaaggaaa
     3901 tttagcatcg cagatccaga aagtgtttat tatgtaaaaa cagaacgaga aaaacagttt
     3961 gcttattctg ctcataccgt tggtgatgaa aatggttttg tattagatgt catgattacg
     4021 gctggaaatc tacatgacag ccgaatgctc gtgccacaaa tcgaacgcgt taaatcacgt
     4081 tatggtgtag ctgctaatgc tgcgtacaaa acaccttgga acgccaagta tttaatcgag
     4141 cgaaaactac gacccatttt cccttatatc agtttcgcac aactactcga gatggctata
     4201 aaaaatatgt atctaaatct tttatttgtg aaagttatcc attattgaaa aattgtacag
     4261 aaagccaaaa acaccaaaaa gaaatacatc gacatatttg ggaagactac ttagatgaag
     4321 cagaacattt acgccatacg gtgtacaatc gtgaaaaata taaaaaaaga aaagaaacga
     4381 ttgaacgcat ttttgcagac gcaaaggaaa aacatggaat gcgctacacc aagtatcgag
     4441 gcttggaaaa aatcagatgc atacgatgct tgtttttgtc gcaatgaatc tgaaaaaatt
     4501 aaccacttgg cagtgggagc atatgtcgtt atatctttta ttatacaaaa atagacaaaa
     4561 gtatcagtct acactcttag taaagaggaa actgatgctt ttgtctacag tcttagagag
     4621 tgggacaata gtcttttctc caaaagaaaa caagtccgac tttgatagtt aatttcaaaa
     4681 tcggacttgt tttttcttct ttacgtcttt tcctctccat gtatttccct aattttctta
     4741 atttaacgcc atgagtgcca agccaatgtc aatcttggct ccttgtttcc ttcataagtg
     4801 gaaatccgtt tccgatagat cgaaccttct ttttccttta aaagtttttt tacattctgc
     4861 tttgaaataa cgccatgtat tattcactaa aatttgtcgg tttcgctcac tttttgcttt
     4921 cgtacattga ctacgaacag gtcagcaccg accactttca cattcgtaaa ttttgaagac
     4981 acgtataaat ccacttttat cttttcttcg actaaagcgt tgaccgcaaa agcgacatct
     5041 gtttcagaaa tatattgttc catatctaat ggtaaaattg tttggttgat ggtataattt
     5101 ttaaacataa agcactcgct tcctttc
//
LOCUS       DACCSK010000149         5114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCSK010000149 DACCSK010000000
VERSION     DACCSK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.152"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            55..2778
                     /locus_tag="IVP39_002522"
     CDS             55..2778
                     /locus_tag="IVP39_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7587573.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            2791..3423
                     /locus_tag="IVP39_002523"
     CDS             2791..3423
                     /locus_tag="IVP39_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7587574.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            4322..>5114
                     /locus_tag="IVP39_002524"
     CDS             4322..>5114
                     /locus_tag="IVP39_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7587575.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         1742 a          780 c          991 g         1601 t
ORIGIN      
        1 taactagtac atatggtaaa ataactagta catattgtga aagcaggtgg caagatgcag
       61 cgattaaatg actatcttta tcaactaaca ttgcttttga aatctacgcc ttcagataat
      121 tattcagctc gcagtatttc gcaagcttta cagttgaata gaagtacaat cagtagctac
      181 ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa aaagttaccc ggttttattt
      241 ttacatagaa cagctcttga agaactacat atcacactga ataactccga aatagaatca
      301 attgaaagct tgttaacaat ttctcaaaaa cccgcattag atcaagtaat tggctctaaa
      361 ggaagtttga aagaagcaat tgatcaaatt aaaacggcag tcttatatcc tggaaaaggg
      421 ttgcctttat tattgatcgg cgctagtgga tcagggaaaa cctttctagc gaataaaata
      481 tatgaatatg cagtagaaga aaaggtcatt cacacatcgg cacctttcat agattataat
      541 tgtgcacaat atgtgagcaa tccagagtta ctatcctctg ctttatttgg ctacacgaaa
      601 ggagcattta ccggagcaag ccaggagcat actggtttgc tagaaaaagc cgatggagga
      661 gtattgtttt tagatgaagt gcatcgctta tctgaagaag ggcaagagaa attatttaca
      721 tttatggata cgggcgaatt ctctcctatg ggggataata gtattcggaa aaaagccgat
      781 gttcgattag tctttgcaac aacagaaaat atatatacga ctttcttacc aacttttctt
      841 aggagattgc cagttattgt taatctacct agatttcaac aacgtccgtc gtttgaacgt
      901 ctgtcattga ttgatgaatt ttttgtttct gagagtaaaa ttttagctaa ggaactatct
      961 gtttctgatg cattgattca tttcttaatg aatagtgatt ttgaaggtaa cgtggggaat
     1021 attaaaaata ttatcaagta tgcttgcgga aacgcatata tccaccagaa gagtcagcaa
     1081 acaattcaag ttaaattatt ggatatgcct ttagagtaca gttataaatt caaggagcaa
     1141 ctaaataaac ccagaaagaa aagagcagat cgaatctact atccagaaga aacacgacaa
     1201 gtgcaactag aaagaaacaa tcagcttttt gttcaatttt ttgaccaatt acttactgga
     1261 ttctctgaag tgattgaaaa taacaaggat attaaactat ttttagaaga aatggttacc
     1321 aaggtaacac aagtaatgga tacgcttatt tttcaagagg attacgaaaa agagcagtct
     1381 ttatacgcca ttctaaacta tcatattcgt caaagtattg attttatgcg agaaagctac
     1441 gggtttgaac aagatggtaa ccgggtagtc gctttggcca actatctata tgtcaaggat
     1501 aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa ccaaagatca gcttaacgaa
     1561 ttttttgcta atgttcttga aaccccttat tggtatgcaa aaaaaatctt aagcctttta
     1621 tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt tctttattac gctatatttt
     1681 tatagtttga atgtcacgaa tgttaaaaca ggtattaaga gtattgtgct agctcatggt
     1741 tattctacag ctagtagtat ggcgaatgtt gccaaccgta tgctgaataa gaacgttttt
     1801 caagcttatg atatgccttt agatattaca ttagagaaaa ttgaagcaaa attgattcaa
     1861 ttccttggag attatcgaac agattcaggc ttgattttat tggtagatat gggttcacta
     1921 aaccaactag gcggtttatt agtgaatcat ctaaatggac cactgttaat cattgatcat
     1981 atcaatacac cgttattatt ggaagtcggc caacaagtat taaacaataa aagtatcacc
     2041 gaaataggag cagaaattga aacaagtctt actttcaaaa agcagttact acttcccaag
     2101 aaaaagaaaa agaaagccat cattacttgt tgttatacag ggattggtag tgcgacccag
     2161 attcaggaga ttttaacgaa atgcttgaaa gatgcagtta gagaattagt aattattccg
     2221 tatgattaca aaaagctgtt agaaaacaag aattttgaga ctccttttca attatacgat
     2281 gtcatcatga ttgtaggtac agaagatccc aaaattaatc agattccata tattggatta
     2341 gatcgcctga ttaatgggga agcaattggc gaatttgttc attacttgga aaaagagctg
     2401 actattgaag tgaacgattt acaaaaagaa ttaattttca atttttcaat tagtaaaatt
     2461 gtcgaaaatt taaccatttt ggatccagat aaggtgctat cttttgtaca aaaagcaatt
     2521 aaagaattat cgaagttatt aaattttgat ttttctaata atcagctgtt tttgttgtac
     2581 cttcactgta gttgtatgat tgaacgaatt gtacgtaaag aaagtgtgga tgagcaagag
     2641 gacattgaag aatatatgca agcaaatgga gaaataatgg ataaaattca tagcgctttt
     2701 tttgagattg aaagggaata cacaattgaa ataccagatt tagagttgcg attattgagc
     2761 gatattgttc aaggatagga gtgaattcga atgacaatgg gagtaatttt tgatatggat
     2821 ggggtactca tagacagcga gacattttat tttgagagaa gaatgaactt ctttcaagaa
     2881 ctggagatag atccaggatc aaaccagatt gatgattatg tggggaaaac agaagctgca
     2941 atatgggaaa cattagttcc tgaggacaaa tcattaagag aggaacttta tccgaaatat
     3001 gtagattatc gaaaaacgca tccaattgat ttcgctaaag atttacgaaa agaagtgaaa
     3061 ccgttacttg acttcctcaa gaagcgggat ataccattgg cactggcatc ttcttctcca
     3121 gttgttgaga tagagagtat gctcattcaa tgtgaactta ctgaatattt ttcctttgtt
     3181 ataagtggtg agtcattaaa acaaagcaaa ccacatcccg aaatatattt aaaagcgaaa
     3241 cagcaattag actgtgactt ctttattgca gtagaggatt caacgattgg tattaaatca
     3301 gcaaaggcag caaatatcta tacggtggca ctaaaacaac caatgcaaat cgatcagagt
     3361 gaagcagaca tacaaattga aagtctacaa gaactggaac aaattgtttt agaaaaaggt
     3421 tagtgaatta atggaaataa ggctatatat ctgtgctagc tattttgact aaccaaaaaa
     3481 acttgctata attcaggtgt agtaaaaggt gctggtaaca ccttttacga aaatgcttcg
     3541 gcagtgataa tgggattggc tttttaagtt ttttattttg ctcgcaatcc ctaaggtcat
     3601 gtgaatgatc ctagggattg ctagaatatt ttgcaacatt ctatctattt cagaacgtaa
     3661 ccttccccag ttcattggga ttcattagca tacaaccaac atttcaaaaa gaaattgcac
     3721 tacaccatta ttagctattt tgactaaccc caaaaaactt gctataattc aggtgtaggt
     3781 aaaaggtgct ggtatcacct tttacgaaaa tgcttcggca gtggtaatga gtgtggctta
     3841 aaggactagt cctttaagct ttttactttg ttcgcaatct ctaagatcaa gttaatgatc
     3901 gataagattg ctagaatatt ttgcaacatt ctatttcaga acgtaacctt tcccattcca
     3961 ctgtgattca ttggcaaccc tcctcacaac caacgtttca aaagaaattg taaagttgtt
     4021 ggggtaccac gaccgaacaa tactacacct gaatgatagt aagttgtctc attaagtata
     4081 gcatatcctc tttttgggta aatagaaatc ggttacattt tttatccaag accaagtttt
     4141 ttcatttggt cttatttgct atacccaaac aattccgcag tatagtcgac ggtattgatt
     4201 ttatcaggtg agtaatagaa acttgttttc cttccccaaa acattaccta taaaggagaa
     4261 aaaatggtat cgtaattata taggtatagg atgaaatttt tttctaagag gagggttgtt
     4321 gatgaaaaat ttacagttaa aatttattgt tgatccatcg attgtacgct ggtttcggat
     4381 tatcaatcaa tttgagcgca atcaacttgc cacaaaagga gaattagcaa agagtaatca
     4441 tgtatctgaa cgaaccattc aaacagatgt caataaaatg aaggactact tttctgaaag
     4501 tgttcaattt gtttcaacga aaagtgggta ttcttttcag aaggaaagac cggttctttt
     4561 tctcaagcag aaggagcaac tactggagaa cgaaatttta tttgagttgt tagggaatgt
     4621 cttttatggc gaattggaag atatgacgga gctaattgat cgttttcatt tatctgaagc
     4681 gacttttcgt cggtatttga agcagattca accagttttg gaagaatacg gcttagaatt
     4741 gagtctttat cctcttgatt taaaaggaga agaggcaaat attcgcaagt tttttaaaga
     4801 cttctattat gagggggaga tgacgcccca tactcttgtt ccaccaaggg atcttcatga
     4861 acttgtgcaa gctacattct atgataaatt tgagtatttc tcgattggaa cagggacttc
     4921 tcctgctgaa ttttattata gtttgtatat tgcgattgag cgtgtgagac aaggaaaaac
     4981 gatagccatt cctcaaggtc tgattgagcg agtaattgct agtgagaatt ttcgcttaat
     5041 gtattccttg aaagaagcga ttcaaacaaa ttatcaagtt gctttatcag aagaggagtt
     5101 ttgttggttg tatt
//
LOCUS       DACCSK010000150         5112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCSK010000150 DACCSK010000000
VERSION     DACCSK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.153"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1..394)
                     /locus_tag="IVP39_002525"
                     /pseudo
     CDS             complement(1..394)
                     /locus_tag="IVP39_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            415..1615
                     /locus_tag="IVP39_002526"
                     /pseudo
     CDS             415..1615
                     /locus_tag="IVP39_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
     gene            2349..3317
                     /locus_tag="IVP39_002527"
     CDS             2349..3317
                     /locus_tag="IVP39_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7587576.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            3328..4143
                     /locus_tag="IVP39_002528"
     CDS             3328..4143
                     /locus_tag="IVP39_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP7587577.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKMGNEFAGKSCLVDGSFGFGIHYD"
     gene            4211..4921
                     /locus_tag="IVP39_002529"
     CDS             4211..4921
                     /locus_tag="IVP39_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7587578.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKQMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4957..>5112
                     /locus_tag="IVP39_002530"
     CDS             4957..>5112
                     /locus_tag="IVP39_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7587579.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYD"
BASE COUNT         1739 a          769 c         1022 g         1582 t
ORIGIN      
        1 aatgccgttt gagtggctcc catgtcgtgg aatctttgag taagagtttc caatatcgct
       61 tgagccgacg gtacttcttc tgattttcag agtgtgaggt tcgaaaacga ttcatgatct
      121 ggacacgtaa ttgattgaag ctgcgattga tgtgttggac aatatgaaag cggtcagtta
      181 cgatctccgc atttggaaag accgttttaa gcaactgaca atagctggca ttcatgtcca
      241 tcactaggta cttgaccttt aaacgggcct ttctaggaaa gcgcaggaag tattgagtta
      301 gtttgtaaag tctgcgatct tcaagggtcg taaatagctg attggttgtc ccatcaacac
      361 aaataaaact catggaacca gaagtggcaa cattgtgtac ttttctttgc aatcttgtgt
      421 acaaaactat tgcaaaacac agcgcataag aatccttaca aaacagtaga tgaagcaacc
      481 caattagatc ttgttatagt tgttaatcag ctcttaacgg gttacgattc aaaatgggtc
      541 aacaccctat acttagataa gtcacttgaa tatcagaaca taattcaggc tttttcacgc
      601 acaaatcgtc ttaatggtcc tgaaaaaccg ttcggaacca ttcgttatta tcgtaaagca
      661 catacgatgg aacagaacat tgaagaggct attgagttat actcaggcaa acaagcaaaa
      721 gggttatttg ttgaaaaatt atataataac ttagaaaagt tgaacgatat attcgaagaa
      781 attaagtccg tatttaatat ttctggcata gaaaacttca gtcatttacc ggaggttcca
      841 ggggaaatta agcaatttgt aaatctttat aatcaactaa tccattgtta tgaatcagcg
      901 atgatacagg gattcacatg ggcaaaatcg acttatgaaa tccaggatta tgaacctata
      961 acagtagtaa tcacagaata tgaatttaat atcttggcac aacgttacag cgagatagag
     1021 agaacctaga gacggaaatg atagtatagc gtatgacatt gattaccaat tacctgaagg
     1081 tagtcgtagt cgtattaatt atgagtattt aaacacaaac ttcaaaaaat acatgaatat
     1141 aaaagaagaa cgtgattcga atttaattca atcattgtta aacgacttac atcgcacatt
     1201 tgcgcaatta ccagtggagt tacagccata tgctgagatg attctaaatg atgtaaataa
     1261 tggggaatta gttatcaaag aaggatggga atttactgat tacctcaatg aatatcagct
     1321 tagtgaagag gacgatttta tccaagattt agtggattca accaatatta atgaggcatt
     1381 attacgtgag atgttggatt tacgcttaac cgaggtaaac attaatgaat atagtcgatt
     1441 tgataaatta aaatcaagtg ttaacgtagg tcatttcagt ggatatatcg agaaaaattt
     1501 aggtaaaatg gtaataccaa tcaaggtaaa tatgctaatt gacagattat tgcgtgcatt
     1561 tttattagaa gatgtattcg atgttacaga gtacataaag aattacttta actagagtac
     1621 acctacatct tatagtacat aaaaaaattc atctttttta gtttaataat tcaccaaaaa
     1681 ggtgagtaat gccatttttg aaaattgagc ttgttttatg tctacatctg tatccgtata
     1741 taatccaaag gattatcaac tcagaagagt ctttattttt tctgaatata actactgtat
     1801 caaaaacaat cgattatgag acaaaacaaa atgacctctt ttatttaaga aaggaggtca
     1861 ttttttgtat cataaattgt attaaaatca atgtatcact ttttaatcag ttttaagacc
     1921 ataaattgcg tactaaaaga aattctgcaa ttcaaaataa agcattttaa gccattttca
     1981 aatttaagtg cacccatcta cccgtcaaat cactttattt tccactccag tagtttccta
     2041 attcaactgt gtgcaaaaac gatttcttct aaacaaatca agacaaactt ataaaccttc
     2101 atcccagcac cataattatt taaatcatag gcatttttcg aaattagtat atgtactcaa
     2161 gtttcactgc agattattta tattttttag agaaataaat ttttcctatt gacaaacaca
     2221 aagaaagcgg atacaatgca attatgtcac atgacgttat atgacgttat gtaatatctt
     2281 tacatttaat cagttacttt tattttggat aaaattaaca tttttaaaat tttaaggagg
     2341 aaatgttgat ggatgtagca aaaacggtta acgcaattgt agaaaaaaat gatggagaga
     2401 tttctggaat ttatttggtg gcttgtggcg gttcgctggt agatatgtat gtctcagatt
     2461 attttcttaa agccgaagca tcgaagtgta ttacaggctt atatacagcg aacgaattta
     2521 accatgtaac cccaaagcaa ttagatgata aatctgttgt cattctatgt tctcatggag
     2581 gaaatacagc agaaacggta aaagcaggag aagtcgctca agctagagga gctgtgactg
     2641 ttggtctgac tcataataaa actgcggatt tattaaaggt ttctgatcat agtttccttt
     2701 acgaatgggg agatgatgca aaaatagtca ataatcctat ggccatcatt cttgatttaa
     2761 ctgtatcttt ggtcaatgtt accgaaacat ttgaacatta cggtaaattc agagaaggca
     2821 tgaaggttat cgataccgta attgaaaatg gcattaagca ggtgcaggat cgtattaaag
     2881 tgtttgctga taagtacaaa gatgaaacaa tgttttatat cttaggtagt ggaccatcat
     2941 atggacatgc ttatggtttt tcaatttgct ctttgatgga aatgcaatgg cttgattcaa
     3001 cttctgttca ttctggagaa ttcttccatg gaccttttga agtaacagac aaagatacga
     3061 attttattct cttgatgagc attggacgca ctcgtgcttt agatgaacgg gcaaaacgat
     3121 tccttgataa atatgcagaa agagtagaaa tcgttgatgc aaaggaacta ggattggata
     3181 tgatatcaga tgaagtttct gaattcttca atcccatttt attctacagt attctttcag
     3241 aatatcgttc agctttagca gatgtacgca atcatgattt agatgttcgt cgctatatgg
     3301 ggaaagtgga gtactaggag gattaaaatg aaggtattag gcttaggtga taacgtcgtc
     3361 gataaatatg aaaatctggg aattatgtat cctggaggaa attcattgaa ttttgcagtt
     3421 tttgcaaaaa aactggggca tgaaagtagc tttatgggag tttttggctc agatgaggag
     3481 gcagctcatg ttttatcagc aataaaagaa ataggactgg acaacagtca ctctcgcttt
     3541 gaaaaaggcg agaatggctg tgccagagtg aagattgatg agggcgaccg gattttcctt
     3601 ggcagcaatt cgggaggtgt tactcgtgaa tacccgattc agcttactga agaagacaga
     3661 gaatatttaa accagtttga attgattcat atgggattat atagccatgt caatcatcta
     3721 ttagaagagt tacaaaatgt ttctggaaag atttcttatg atttttctga cgactttacg
     3781 gaagaagagg ttcagcgggc gattgataaa gttgattttg gcttcttttc tatagaagac
     3841 ttttccgatg aagaagtaaa gcagtttttg atgaagcatt tttgtgagcg aaatgatgta
     3901 ttgattgcaa cccgaggaga aaaaacggct attggttatg atggagcaaa gttttatgaa
     3961 gtgaaccccg tgctagaaga accagttgat acaatggcag cgggagattc tttcttaaca
     4021 gctttcctac tttattttct cgaaggggac gatatagtca ccgcgatgaa aatggggaat
     4081 gaatttgctg ggaagtcttg tctggttgac ggttcgttcg gctttggtat tcattacgat
     4141 tgagcttgtt ttaaattcct gcatttgata acctaaagaa agaaatcccg gtaaagtgga
     4201 ggttaaaaaa atgctggata caaatgcaca attaccattg tacttacaat taaaagaaat
     4261 attaaaaaat aaaatcaata caggggagta tcccgagaat acgaaaattc ccgctgaatc
     4321 agagctgata gaaagttatg aggtcagccg aatcacagtg agaaaagcag tagaagaact
     4381 ggtagaagaa gggtacctac ttaaaaaaca agggaaagga acttttgtca accggcataa
     4441 agtttttcgt aaaattgaat atgttatggg tttcagtgag agttgtaaat ctaatggttt
     4501 tacaccgact agcattgttt tggaacgcaa gatcattcca gcaactgaag ggatggcaaa
     4561 aaaactcgat atcgctcaag gtgatgatgt tttatatatc cagagaaagc aaatggcgga
     4621 taacatcccc attatgttag aaaacaatta tttttcttat gaaagatata gtttcttaat
     4681 ggaggaagag ctgactgatt ctttgtatga agtacttaaa gtccaaggta ccttagccat
     4741 caatcctggg gaaactgcac ttgaaattgt cacagctgat gaccaaattg ccaaaataat
     4801 ggaagtagct gttgggacgc cctttttctt tatggatact ttaatcaacg atcaaaacaa
     4861 cataccaatt catgtaggta atcaatatta cctgggagaa gtatatcgtt tttacttatg
     4921 atttcctgtt ttgatttcaa ggagtagatt ttatttatga agcaaatatt gcggtcaaaa
     4981 atgatttttg atggagaaca gccacctttc aaaggttata ttgtaatcaa tgaagggatc
     5041 attgaaaagg tggaaaacaa ctggaattac agtgatctat taaatgaaaa cacggaatta
     5101 attgcttatg at
//
LOCUS       DACCSK010000151         5108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCSK010000151 DACCSK010000000
VERSION     DACCSK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.154"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            338..706
                     /locus_tag="IVP39_002531"
     CDS             338..706
                     /locus_tag="IVP39_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7587580.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            886..1827
                     /locus_tag="IVP39_002532"
     CDS             886..1827
                     /locus_tag="IVP39_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7587581.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1896..2627
                     /locus_tag="IVP39_002533"
     CDS             1896..2627
                     /locus_tag="IVP39_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7587582.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2771..4951
                     /locus_tag="IVP39_002534"
     CDS             2771..4951
                     /locus_tag="IVP39_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7587583.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4971..>5108
                     /locus_tag="IVP39_002535"
     CDS             4971..>5108
                     /locus_tag="IVP39_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7587584.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HL"
BASE COUNT         1706 a          899 c         1128 g         1375 t
ORIGIN      
        1 tcactctgag tcacaaagag caatttcaaa aaatagcttc tcatgtttgt tgaatattgt
       61 gtcttgtgac cgaggagttg tcaggagaac accgtttacc agaggttgtg acttgttttt
      121 agtcacgacc tcttttttta tttattccat ataaatacac attatttatt ccgaaaagtg
      181 aaagcgtaaa taacaaaaac aaccaactta tttattatta aagaaaagcg tatttaagta
      241 ggacgattct gttgagagct ttcgtagatt ttcccatttt cctatggtaa actgacaaag
      301 ttgagaatgt tagaacagat cgtttaggag gaaacagatg tttacgaatg aaataaaaat
      361 catgctttat gtggacgatg tagaaaaaaa tgcaacgttt tggagagcag ttggctttgc
      421 ggaaaaagat cgtcaagaaa tggatgggac gcttataatt gaagtaggag ttgaggacag
      481 tcaaacctca tttctgttat atgatcgagc atttgttgag tcccattcac ctggcacagc
      541 tactagtgcc ccatctttga tgttttacag taaagatatc tttggactat ataaaaaaat
      601 gcagacgcaa tcagtcacat taggtgatat ggtacagctt ggagaagaat atgtctttaa
      661 ctttgctgat cccgatggca actactttgc ggttacaggt aaataataga aataaagact
      721 ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa ttcaaataat aatattatgt
      781 aaacaaaaaa ctttccatgc gtaattatac acaagcaaat aaatcgtcaa atgcgaacgc
      841 cttctttata ctgtttagga acgaaaagaa ggaaggaaga aatacatgga ttatcaagta
      901 ctgctttatt acaattatac aaaaatcgaa gaaccagagg aatttgccaa aaatcattta
      961 gccttttgca aatctttgga tcttaaaggt cgtattctag tagctgaaga aggaataaat
     1021 ggtactgtgt ctggaaccgt tgaaaacacg actgcctata tgaaagcaat gaaagctgat
     1081 cctcgatttg ctgatacgat gttcaagatc gatccaagcg aaggacatgc ctttaagaag
     1141 atgtttgtaa gataccgtcc agagcttgta agtttaaagc tagaagagga cattgatcca
     1201 aaccaagtga caggtgccta tctttcaccg caagaattta aagaagctat tttagatgaa
     1261 cagacagtgg tgattgatgc acggaatgat tatgaatatg acttgggaca ttttcgaggg
     1321 gctgtacgcc cagatatacg cagttttcga gagcttccag actggatccg cgagcacaaa
     1381 gaagaattta tggaaaaacg ggtagtgacc tactgtactg gtggcattcg ttgcgagaag
     1441 ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg gacagctgca tggcggtatt
     1501 gctacttatg gtaaggatcc tgaagtccaa ggagaattat gggacggcaa gatgtatgtg
     1561 tttgatgaac ggatcgctgt cgatatcaac caagtagata aacaaatcgt cggtcgcgac
     1621 tggtttgacg gaacaccatg tgaacgatat atcaattgta gcaatcctga gtgcaatcgt
     1681 cagattcttg cttccattga aaatgaagaa aaacatttag gagcatgtag catagagtgt
     1741 gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa tctctccaga agaagcagaa
     1801 ctgcggttta cagctttaat taaatagaaa acaatagttt tgattgtcga ccatttccaa
     1861 tatgcgctat actagcgttg aggatggtga tccacatgaa atcattgatt tatgctattg
     1921 gggatgtcca tggcaaacat gagctttttg aaaaacttat tcactattat gatccaaaga
     1981 tccatcagct tgttttatta ggggatctca atgatcgagg tcctcgtaca aaagaatgtt
     2041 ttttattagg aaagcaatta gtcgaagaaa caggagccat ttacctccgg ggaaaccatg
     2101 aagaatattt tttgcaattt ctgcaatcac cggaggactg gtatgcgcct tatatgagaa
     2161 atggtggaaa agaaacgttt gaaagcctga ttcacccagg ggcatcggag gaatactccc
     2221 caactgaaat ctcgatgatg atccaatcga gatattcaga actgattaaa tttttagtcc
     2281 agcgcccctt gtattatgaa tgggaaaatt atctttttgt ccatgccggt gtggatttga
     2341 caaagaaaga ctggcgtaag acaagtctaa gagactttat ttggataagg gaaccatttc
     2401 atgaagggaa aaacaataca ggtaagacaa ttgtatttgg acatacgatt acaccaatgc
     2461 tccatggaga tatgcagaca acagacctct ggattcagga tcataaaatc ggaatggacg
     2521 gtggggctgt attcggaggg tcattgcatg gggttgtttt tgacaaggaa ggtattcttc
     2581 aagatattga atatcagaac aacgaaggac cttggcatgg aatctgacta atggaaagaa
     2641 tgaaggagct gtgactttgt tgcggctctt tttattttta tagagaatat tgtttctttc
     2701 agtagtttaa cccaggataa aaagtatgat agaataaaga agcaattttt agcagaaaag
     2761 aggcatttta atgacggaaa atttgaacca gacgatcatt cagcttgtac aaaaagaact
     2821 ttcagactat cgtcctaagc agttgactac tgtacttaat ttgttaaatg aaggaaacac
     2881 tgttcctttt atcgcgcgtt accgaaaaga aatgactgga agtcttgatg aagtccagat
     2941 tcgtgaaatc gaagagcggt atgcctatct tgaaaactta gaaaaacgta aaactgaagt
     3001 gattcggcta atcgatgaac aagggaaact gactcccgag ttagaagcag aaatcaccca
     3061 agccgtgaaa atgcaacaag tggaagattt gtatcgtcca tacaaacaaa aacgacggac
     3121 aaaagcaacg attgctaaag aaaaaggact tgaaccactc gcgaagtggc tgatgcagct
     3181 tacagatgga gaggtgcaat ctgaagcaga aaaatatatt gacaaagaaa aagaagtctc
     3241 ttctgccgaa gaagcgttgc acggtgctca tgagattatt gctgaacaag tcagcgataa
     3301 tgcaaaattt cggacttgga ttcggtctta tacgtataac aaaggtatgt atgtcagcaa
     3361 tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa atgtattatg actttgcaga
     3421 acccgtccat aaaatggttt ctcatcgaat attagctact aatcgtggag aaaaagaaga
     3481 tattctgaaa gtattctttc aagtagatga agcagcaatt ttagcttact tggatcgcca
     3541 aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc agagaagcct atcaggatag
     3601 ttataaacgc tttattcagc cagcaatcga gcgagaactg cgcaatgaat tgactgaaaa
     3661 ggcagatgag caagcaatcg ctattttcgg cgaaaacctc cgtaatcttt tgttgcaacc
     3721 gccactaaaa ggtaaagttg tcctgggatt tgatccggct tatcgtactg gatgtaaact
     3781 agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa gttatttatc ctcataaacc
     3841 tgcagcgcaa gcaaaaagag aagctgctgg accagctttt atccaattaa ttaataaata
     3901 tcaagtagat atggtagcta tcggaaatgg tacagccagt cgggaatctg aactttttgt
     3961 ggctgagcag ttgaaatccg ccgaccataa aacctattac gctatcgtca acgaagcagg
     4021 cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa tttccgcatc tacaagttga
     4081 agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat cctttagctg aattagtcaa
     4141 aatcgatcct aaagctgtcg gagtaggcca gtaccaacat gacgtttctc aaaaacgttt
     4201 ggctgaacaa ttggattttg ttgtggaaac agcggttaac caagtaggtg tggatgtgaa
     4261 tacagcaagc ccgcagctct tgcagcatat atcaggattg aataaaacaa ctgcccaaaa
     4321 cattgtcatt tatcgtgaag aaaacggaga attcaccgca cgaactcaat tgaaaaaagt
     4381 gccacgctta ggaccaaaag catatgaaca agcaatcgga ttcttgcgag tccctggtgg
     4441 gaaaaacatt ttggacaaca caggtatcca tcccgaaagt tattctattg caaaagaaat
     4501 attaacttca gtccaacttt cagaaaaaga gcttggtaca gaagaagcag tagaaaaatt
     4561 gacacgatta tccgtagaga aactggctga aagcctatca gtcggagaag aaacgttagc
     4621 cgatatttta gctgggctga cacaacctgg acgagatatg cgtgatgaga tgccagcgcc
     4681 cttattacgt acagatgtat tgagtatgga agacttgaaa ccagggatgg aattgactgg
     4741 aacggtccgt aatgtcattg acttcggcgc atttgttgat atcggtgtga agcaagacgg
     4801 acttgtccat atatcgaaac tcagcaaaaa attcgtcaag catcctacag atgtcgtgtc
     4861 agtaggagat atcgtaacag tttggataga acaagtcgat acgaaaaaag gcagaatcag
     4921 tttgacgatg ctttcccctt atgaagagta acaatgagga gtaacaaaaa ttgacacaag
     4981 aagaactgca gcagttagta gaagagatat cattaaaaag cttccagctt cctttcaagc
     5041 accaagcaat gttcaatcca cgcttacgta caactggcgg gcgctatcat ttaaacgatc
     5101 atcattta
//
LOCUS       DACCSK010000152         5088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCSK010000152 DACCSK010000000
VERSION     DACCSK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.155"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IVP39_002536"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IVP39_002536"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP7587585.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IVP39_002537"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IVP39_002537"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP7587586.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IVP39_002538"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IVP39_002538"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP7587587.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IVP39_002539"
     CDS             complement(4028..5044)
                     /locus_tag="IVP39_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7587588.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1271 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaac
//
LOCUS       DACCSK010000153         4860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCSK010000153 DACCSK010000000
VERSION     DACCSK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.157"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..1217)
                     /locus_tag="IVP39_002540"
     CDS             complement(<1..1217)
                     /locus_tag="IVP39_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7587589.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVE"
     gene            1278..1853
                     /locus_tag="IVP39_002541"
                     /pseudo
     CDS             1278..1853
                     /locus_tag="IVP39_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2034..3227)
                     /locus_tag="IVP39_002542"
     CDS             complement(2034..3227)
                     /locus_tag="IVP39_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7587590.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3311..3514)
                     /locus_tag="IVP39_002543"
     CDS             complement(3311..3514)
                     /locus_tag="IVP39_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7587591.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            3771..4013
                     /locus_tag="IVP39_002544"
     CDS             3771..4013
                     /locus_tag="IVP39_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587592.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(3935..4168)
                     /locus_tag="IVP39_002545"
     CDS             complement(3935..4168)
                     /locus_tag="IVP39_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587593.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4175..4606)
                     /locus_tag="IVP39_002546"
     CDS             complement(4175..4606)
                     /locus_tag="IVP39_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7587594.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1324 a         1109 c         1004 g         1423 t
ORIGIN      
        1 atttctacaa aaaaatagtg attctgtttt gaaaattcga tttttagttc tgcattttcg
       61 taaacatcca acattttttt cgcactgtat agacctaacc cgccattttt tttagaggtt
      121 tttcctcttt tgaccatctc cgatagcgtc aaattcgttg gatttgcctt gttcgctata
      181 ctaaaacgtg atccctcttt ttcttccagc agattgatcg taatcggact ctgatcggct
      241 acacgatgat cgaatgcgtt agaaataaaa atagataaac agcgaataaa aacaaccaga
      301 tcaatcggga ttgttttcac ttcgacagga ataactactt ggatcgggat tttctccgat
      361 tctgcttttt ctagaaattt taaaaagaca cccctgacag ctggggatac aatatttgtt
      421 agctgttgta tttgcggttt ttcaaaaata gctgcagaat gatcttctaa tatttctaaa
      481 aattccaacg tttctttttt attttcctga agagtaattt ttaatgaaaa cagtagactt
      541 ttataatcat gtttgaattc ctctagatga tggtaatatt cttcatttat ttgatacagt
      601 tgaagcgatt cctgatgttg tctttctttt acttggaaac tgatcaacat aatggaaaaa
      661 aaaaaagaaa aaaaactgat gactatcgac aaaagagata gaagcagata attagatgag
      721 cggaagtgaa cacttcccaa taccaagtaa atcacgcccc agcttaataa aatacaaata
      781 cctccaaaat agtgttcagt tggataagta aaaagcaagt tgagaaatct cgtctgtttg
      841 tctatacttt ttactaatag atagaaaata aatgcgataa gtagtgcaaa aaaacttgct
      901 gttccaagat gtctctttcc taaataatcc atcagatcaa aaaccagaaa aaagctgagt
      961 ccagtgatag caaaaaaaag attactgatc aaacagctga taaacaggtt tttcgtagta
     1021 aaataaaaaa gaaacatcaa taataagcca actaggtaag gaaagctcac tttagggata
     1081 ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa agctgttttt tactagcagt
     1141 tgtttcgaaa agaataaata gatacctaaa tctaaaatca aagtaaaggt caaataaagt
     1201 gtcactgttg cgaccatctt attcctccat tgttttgttt caaaatgtac acatattttt
     1261 ttaataggag cctgcatatg aaaaagtatt ttatcataga agataaccag tttcacagcg
     1321 aatggatcat cagagaattg gtcaatcatc atatcgagaa tatcgtgcag ctcacttcta
     1381 tctctcaaat caatcagttg atccagttgg gcactattga taatcacgat gttttcttta
     1441 tcgatatcga cttgaagcaa tcactcacag gtattgatgt cgccgaacaa atacgtaaaa
     1501 aaaatgcaca tagtaatatt gtttttttca ccgcttatac gaattttgca gtagagatca
     1561 tcaatcggaa aatccttccg attggatata tcagaaaaga caggaattta tctgaacaaa
     1621 ttcgtcaagt gctagaacag atcaattgga aagaacagca gatcgtaaat gtcgataaaa
     1681 tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc tcgggaaatt ttatatattg
     1741 aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac aactacagaa gataaattag
     1801 tgaatacttc tttaaaacaa ttgaagacag agttagaaaa gtatgcttac tttttcttaa
     1861 aaattatatg ggattttaga ttatcttttt agaagttctt gaatgtcggt atagcccgtg
     1921 tttccgggct tttccggcat ttttgatatt gttagatact tacaaatgtt cttattacct
     1981 ctcaccattt ccccctcaaa agtagtaaat ggagtagtaa aaccggcctt gtgtcaggct
     2041 gccagccgtt ccatttctgc ccttgcggaa tcgaatgtgg cgtgtgcata atagttcagc
     2101 gtcatggtga tattggaatg tcccataatg tactgcaagg ctttggggtt cattcccgca
     2161 ttggctaacc gggtgcagaa tgtatgacgc atagcatggg gcgtcgtcgt tttcggcaac
     2221 gcttcttcat ggctcttgtt gtacttcttc acaagcctgc caaacatccc gccataatct
     2281 accgccgtca tgggatagcc gttctgtttg agaaacagga aattggcata cccgtcaata
     2341 atgaacggct ttgcaccttt gcggtttttt atcactcgct ggaacgcctg atacacttcc
     2401 tcattcatgg ggatttttct gacgccgctt tgggtcttgg gttcgtcaat atagtaaccg
     2461 acgccggtat ttctcaaaag ctggtggctc acgatgatta cccggttttc aaaatccaaa
     2521 tctttgtccg tcagtccgca cagttccgaa atgcgaagcc ctgtccccag cagaataacg
     2581 attgcgtcat aatgcttttg gtaaacctta tcaccttgaa taaaggacag aaaactttct
     2641 tcctgtgctg gtgtaagagg cacctttggt tccgtatcgt cgtccagaac gtcactaagc
     2701 tggaagtcaa acgggttttt tcggatgcaa tcgtcctgta tggcggtgta aaaagccgct
     2761 ttcagggaac gcttgtcgtt attgatggtc ttataagaaa ggcccttttc tttcatccga
     2821 agcgcccatt cctttgcgtc ggacagcttc acactgtcaa tcgggcagct ccccagccga
     2881 tcttgttcca gaatacgcat cagccgttcc cggcccagtt tggtgttgtg cttcacattg
     2941 ccccggtgcc tgatctgctt ggcgtagagc tggcagacgg tcattttctt gccgatggtg
     3001 tctatcccgt cgtcaaggtc tttctggatt tctttctcct tttcccggag tgaaatatcc
     3061 tcccgtttac ctgccggggt cttatccgta ggcactaact tccatgagta aacaaactgc
     3121 ggttttccaa aggtatcaac atatttgtaa gcatatcgtc cgtcttttct ctggctctct
     3181 ccagaccgca aaacgcgatt tttgctgtct cgtctttttt ctgacatctc gtcatgctcc
     3241 tttccatgac ggaaagagcc gcatatatgc ttataaatta ttataacata tctgcggccc
     3301 tttttcacta ttagattgtg tccaaagtgt caattatttt ctcaaactgc ttgcgtttga
     3361 tctggatgcg gttgccattc ataatgaccc agccagaggc cggattttcc tcggccagct
     3421 tacgcaattt gttttctccg atacggaaat atttagacgc ttcctcaatc gtcagcgtgt
     3481 atttttccca aataggcaca tcatggttca taatgcagcc ccctttcaca aattcaaagg
     3541 tttgtgtgat ttaggggcat agacggacgg ctgctggcac agctccacgg gactttcacc
     3601 cccgcgtggt tctcacgcgg ccctacccat tgcctgcgac gcttttaacg ctcggactgt
     3661 ggctataagg gagtatcata gtcctgcctg tacgtcgtcg cccgcaagcc gccacgctca
     3721 cattgcggcg tggtgttcct cgctccgctt ttcccgtagg gaaaagtatc atggcgcacc
     3781 agccgtccgg ctcggcacag acagaatgtg tccgtatgcc ctatgatgcg ccgccgttat
     3841 cttgcgggca tatttatcaa ggtgcgatca ggccaccggg aaaacaccga aacggggaaa
     3901 gggtaagccg tttcatgctg ccggggccgg ttcgtcagtt cgtgacgata gcgtggatga
     3961 gcttgatttc cagacggcgc ttcatggcct cgtccagaca cacatgcgga tagccgtttt
     4021 cgtcatagag cgtccgggta cacagcttgt tcatgtaacc gctgtaatac tgcaaaacgc
     4081 gctctacggc tccggcatcc ccagcgcggg cggcctcgat caccgaaagg ggcaaaagct
     4141 caatgccctt gtagttcggg ttcttcatcc gcgtctaacc tcccttcggc atcaacgcct
     4201 ttagctgttt ccgcagctcc gataaggtat ttgtcctgtg ccgctgaacg gcactccggg
     4261 acattcccac aaggccgccg atctcaccat ctgccatatc cagaacacag tgcaggatta
     4321 aaatactctg ctccatgctg ggcagcgcgg caaaggcttc ggcaacaagc tcattgttga
     4381 tatgtaggtc atagccatgc gacgaaaacg taaagctgtc gctggggtag tgatctactg
     4441 tacagagctt gtccaaatct gcctgtgaca aagagcttag tgacacttca cggtcacggc
     4501 ggcgcttcat ttcagccaag tagcttttgg cctcgttacg cagtaccgtc ttgcaaaaag
     4561 catcgaacat gcaccgcgct tcatagtcgc gggggatagc atccatcttc tcaccccctt
     4621 tctgtggggg acttaggtgg ttctctccct ttccgctaac aagactttga caaggccgcc
     4681 agctacccgc taaatacctc ctttttgaaa atttctcaaa aaattttttt ggggacagcc
     4741 gggccgcaaa agacgacaaa aatcgcccgg caggtcgcaa acctaccggg cgagaaaatg
     4801 ctttgctatg ctgctgattt acatggtata gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DACCSK010000154         4835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCSK010000154 DACCSK010000000
VERSION     DACCSK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.158"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(21..983)
                     /locus_tag="IVP39_002547"
     CDS             complement(21..983)
                     /locus_tag="IVP39_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7587595.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(985..2424)
                     /locus_tag="IVP39_002548"
     CDS             complement(985..2424)
                     /locus_tag="IVP39_002548"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7587596.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2609..4555
                     /locus_tag="IVP39_002549"
     CDS             2609..4555
                     /locus_tag="IVP39_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7587597.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1564 a          805 c          786 g         1680 t
ORIGIN      
        1 attaaaaaat taatttaacg ttaaatactt cctccagaaa gtattgagat tggtaaaata
       61 taatccttat ttgtcttttc gcctttaatt tttttcagaa gaatatcaac aataagactt
      121 gcaatttcat caattggttg tcttattgtt gtcaattgtg ggaaaaattt ctcaataaaa
      181 gaggtaccat cgtatccaat aatttttaaa tcttctggga tattaagttt aagttgatga
      241 gcaattttca tagtgagaat ggctgttaaa tcatcagaaa caaaaatacc atctggtttg
      301 tgatttataa tagttgactt tatttccatt tcacgacgga tagtagacag attattagga
      361 attttgaaaa tttttccatt tggattttga aaagaaaaac ctagtgcacg taattctgtg
      421 ggagaatccg tgttatcatt tcctgtaatc ataatggtat tttgacaacc atttttttgt
      481 aaagtcttag ctgcaagttt tcccccttca aagttatctg aagaaacaat ggaaacatta
      541 ggagctaaat ttctatcaaa agcaacaata ggtgctctca cacgctcata atcagcaatt
      601 ccaagattat ggcttgatga gattatgcca tcaacttggt tggcttcaag catttcaatg
      661 tattctctct ctttagcagg atcattttga ctattgcaaa taatcgtttt atatccatgt
      721 ttaaataact ctatttcgag atactcaatg agttcagagt aaaagatatt actaatatta
      781 ggaaaaataa gaccaataag ctgaggtgac tttccttgaa gtccacgtgc taaattattt
      841 ggtttataac caagagtttt catagcttca tgaacttttt gttttgtttt ttctgataaa
      901 tatcccttat tattaatgac gcgtgaaacg gttgttgggc taactccagc taattcagcg
      961 acatcagtta attttgctac cattttaata ttttaattca aagtattttc cagtaggttc
     1021 gcctgatttg atttgaatac cattttggtg attttcaggg aatacacgac tagtaaacac
     1081 tttttctcct ttattaataa aaatctcaat gattgagtta tctacaaaaa tgttagcagt
     1141 tgtttcttta gtatcaattg agcattcacg aatcgtacca aagtcagtgg catattgaat
     1201 gcctgctttg ctgcgatcta aaataatttt tccttctttg gtatctacaa tcaagctaag
     1261 tccatttcct ttactatcag agaaaagaag taactccgat ttctgatttg gaggaaaagt
     1321 tagttcaagc tcataagtat tatttgtttc tgcttttgct gtaaattgtt cagaactagc
     1381 acgtaatgat gtaatagctt ctactggata ttggtaaagt ttaccatctt ttattgaaag
     1441 ttcttttaca agactcattg ctccctgata atcatactta tcagttggat aatcgatatc
     1501 aggtagtcca atccaactga caatcagagc acgaccatca ggagtattga atccttgagt
     1561 cgcatacgct tcaaatccat aatcaagatt ttgaatttct gaagcgccca caagttttac
     1621 tttttctatg tcaagcgatt gacagatctt aaaagtattc ggatagatat tcccataatt
     1681 tagctcagat ttatccaacc cttgaggaca ataaatgaga acaggttttt tgtctacaaa
     1741 tactagattt ggacactcaa tcatgtattc tgaaccagtg ccgccaaaat caaggttgcc
     1801 aacttcctcc catgtttcca cgttattgtc atccgctttg taaagtttga taaagcctga
     1861 tttttctaaa ttttgtgctc caacaatcgc atacaattgt ccttcataat taaacatttg
     1921 tggatcacga aaatgatcag ttacatcagc aggtttatga ataagaacat tctcaccttt
     1981 cgtaatgttg tagtctttat ccatccatgc tccgatttga agtgtatcac gttcccaatt
     2041 ttcatttcga acgtttcctg tatagagtaa gaatagattc ccctctattt catatgcaga
     2101 accggaataa gcgccgtggc tatctcgttt tgtgtctgga agaagttctg ctcctgttac
     2161 atgaaaatga actaaatcat ctgattcatt gtgtacccat tctttcaacc catgagcagc
     2221 tccaaatggc caattttgat aaaaaagcgt atattttccg ttaaaaaaag aaaaaccatt
     2281 aggatcgttt aacaatcctg ttttaggttc gatatgatag ctagcacgcc aaggtgatcg
     2341 ttttacatta tccttaattt tatccatctc atctttagtc caatcagaat aagcacgata
     2401 tcgtatttct cgaggtaagt tcatatttta ctccttcaat ttatcttttt acaactagta
     2461 tggtaaacgt tttccgttga aaaatcaatc atttcattta aaaaaataaa ctatatgaca
     2521 aacgtttgac atatttttaa gatatgctaa aataaatttg catttcagtg aaccgttttc
     2581 acgtaaaaat aaaaaaggag tgattcgaat gaattacagt aaagtagctt ccgacgtaat
     2641 acaagcggtc ggaaaagata accttattgc agctgcacac tgcgctacac gtcttcgtct
     2701 cgtactaaaa gatgacacga aagtgaatca aaaagcttta gacgaaaatc cagatgtgaa
     2761 aagcacattt aaaattgatg gacaatatca agtgattatc ggtgcaggtg atgttaattt
     2821 cgtttatgac gaacttatca aaaaaactgg actttcggaa atttcaacag atgatttaaa
     2881 acaaattgct agtaaagata aaaaattcaa ccccattatg gcattaatta aattattatc
     2941 agatattttt gttccaatta tacctgccct tgttgcaggt ggtttattaa tggcattaca
     3001 aaacttttta acagctgctg gactttttgg accaaaatca attgaagaaa tgtatcctgc
     3061 gattgaaggt atttcctcaa tgatccaact gatgtccgct gcaccattca tgttcttacc
     3121 aattttagta ggtatttcag ctgctaaacg ttttggagca aaccaatttc ttggtgctgc
     3181 aattggtatg atcatgacaa caccagatct tggtggctca actgaatatt ggaatatttt
     3241 tggttatcat gtttcacaaa caaactatgc ttatcaagtt attccagttt tagcagctgt
     3301 ttggatatta tctatattag aaaaattctt tcataaaaaa ttgccatcat ctattgactt
     3361 tacattcacg ccattattat cagtaatgat tactggattt ttgactttca cagttattgg
     3421 ccctgtaatg ttattgttgt ctaatggaat tacagacatt attgtttggc tatataacac
     3481 tactggattt attggaatgg gtatttttgg aggaacatat tcacttatag tcatgactgg
     3541 tttacatcaa tcatttccag ctattgaaac tcaattactt tcagcgtgga caaatggtat
     3601 cggacatgga gactttatct ttgtagttgc atctatggca aacgttgctc aaggtgctgc
     3661 aacatttgcc atttggttct taactaaaaa ttctaagaca aaaagtcttg cttcctctgc
     3721 gggtgtctca gcacttcttg gaattacaga gcctgccttg ttcggagtta acttaaaata
     3781 ccgctttcca ttcttttgtg ctttaattgg ttcaggaatt gctacagcag tagcaggatt
     3841 acttaaagta gttgctgttt cacttggttc agcaggtttc ttgggatttc tttcaattaa
     3901 tgcaacatct attccatttt acgtattgtg tgaattgatt agctttgtag ttgcttttgc
     3961 aattacttac ttctacggta aaacaaaagc tgctggtatc tttaaagcag aagaaattgc
     4021 tgaacaaaca ccagttaatt cttcaaaagt agcaacaaca gatccaatat ctactgatgt
     4081 tgaatcaaca caaacaaaag tttcatcaga aacaattgtt agtcccattt ctggtaaagt
     4141 gattagtctt tcttcagtaa atgatccagt cttttcatca gaagctatgg gaaaagggat
     4201 tgccattaaa ccaaatggaa acacggtcca ttctccagta gatggtacta ttcaactcac
     4261 gtttgaaact ggacatgctt atggtattaa atcaaataat ggtgctgaag ttctaattca
     4321 tattggtatt gatactgtat caatgggagg aaaaggattt acacaaaaag ttactactaa
     4381 tcaaaaagtt agaaaaggcg atattcttgg agcatttgac cgttcaatga ttgagaaaga
     4441 aggtcttgat gatacaacaa tgattattgt gaccaatact tcggattatt cagaagtaaa
     4501 tatagtagct gaaaatacag tcactgaggg tactgattta cttgaaatca aataaatata
     4561 tttccctgag taaagatgga attttctttc caaaatcaaa taatatagat taatcaaata
     4621 tttgagaata acattttatt taatcttttc atatatcaat tgtatgaaaa attattagaa
     4681 ttttaaaaaa tgataaaaat tcaaaaagaa aaaatagtag ttatttactt gtcatgttac
     4741 taaataaaaa aataatttaa atatgatata tagtcattta tttagtgatt tcattctaaa
     4801 ataaagggag aaatgtctat gctatacggt gcaat
//
LOCUS       DACCSK010000155         4813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCSK010000155 DACCSK010000000
VERSION     DACCSK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.159"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..122
                     /locus_tag="IVP39_002550"
     CDS             <1..122
                     /locus_tag="IVP39_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7587598.1"
                     /translation="NYFGKRLNMDFNKQLDSFSLENEIAVLDYNIKLKNCFFS"
     gene            156..1094
                     /locus_tag="IVP39_002551"
     CDS             156..1094
                     /locus_tag="IVP39_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7587599.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            1172..2065
                     /locus_tag="IVP39_002552"
     CDS             1172..2065
                     /locus_tag="IVP39_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7587600.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            2103..2666
                     /gene="amaP"
                     /locus_tag="IVP39_002553"
     CDS             2103..2666
                     /gene="amaP"
                     /locus_tag="IVP39_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7587601.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            2679..2867
                     /locus_tag="IVP39_002554"
     CDS             2679..2867
                     /locus_tag="IVP39_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7587602.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            2880..3782
                     /locus_tag="IVP39_002555"
     CDS             2880..3782
                     /locus_tag="IVP39_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7587603.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            3814..4056
                     /locus_tag="IVP39_002556"
     CDS             3814..4056
                     /locus_tag="IVP39_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7587604.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(4312..4683)
                     /locus_tag="IVP39_002557"
     CDS             complement(4312..4683)
                     /locus_tag="IVP39_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587605.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         1747 a          828 c          970 g         1268 t
ORIGIN      
        1 aaaattattt cggtaaacga ttgaacatgg acttcaacaa acagcttgac agcttttcat
       61 tagaaaatga aatcgcagta ctcgattata atatcaaatt aaagaactgc ttcttcagct
      121 aatagtttcg agaaagtcca aaaaggaggt atacattgag atactacttt gtggatgaag
      181 aaaagaatgt aatagaaaca gatgaaaaaa aatataattg gctcgtactt gatagtgcaa
      241 agcccgaaga gattgagcaa gcaatcgaag aattcgagct tcctgatgat attttcgtag
      301 gaaccgaata tccggaagaa gtttctcgga tggagcgcct ttatgagacg aaactagagc
      361 atccattttc tttggtagtg attaatctag atcagtcaga taatcgtatc gaaaaaaaat
      421 taacgccaat atcttttgta ctttccgata atctcttgat tacttgtatc gatagaaaaa
      481 ccgaatttgt ggatcggctg ctgacaaagc atggaaatca tttaaactcg tttgaaaaga
      541 tcatcacgta tacattatta gatatttaca cccattacgt gaaagaacta agagagatga
      601 aaaagcgaat tgacgctttg gataaagcag ctcgtaaaac aacggaaaat gaagaactgt
      661 ataaacaggc agatcttgaa agagacatcg tatatatcga tcatacctta agagatcaac
      721 gggaaacaat ggatcaatta tggaattctg aaactttcat caaacgttta gacgatgaac
      781 agctactcta tgatgtccat ttgagacagc gacaaacaga aaaaatgatc gagatctatc
      841 gagatttatt agagagtatt ggtgatctat tcaacgggat gatggacaat aatctaaatc
      901 atatcatgaa atatctagat tcagctgcat taatcatttc cgttccggcg ttgttcgcag
      961 ggatttgggg aatgaataca ggaggattac cgggtaaaag ttctgctcta ggctttatac
     1021 tcgtagtctt tggctctttg atagcagcag tagttttagg ttaccattta taccggaaag
     1081 attatacaaa ttaaagaatc tatttaaaac aggtatcaaa caaagaaatt tgttttggca
     1141 atacaattta aaaactagga ggaatacagc aatgacagaa ccaaaattaa tcgacccgcg
     1201 aaacttgtac catacggaga aatttcctaa acaagatcag gatacaccag ctttacagca
     1261 gaatatgatt cctgttcctg attgtggaga agaatcctat gaaggcaata atcaactaga
     1321 aaaccgccgt gtactaatca ctggtggaga ttcagggatc ggtcgtgcag cagcgattgc
     1381 atttgctaga gaaggggcag atatcgctct tcaattcttc ccaggagaag aaaaagatgc
     1441 agaggaagta gcaaaatacg tgaaagatgc aggacgtaag attgtcttac taccttttga
     1501 tcttcgtgac gaacaagcac ctaaagaaat catagataaa gccgtgagtg aacttggcgg
     1561 gctagatacg atggtactaa acgcaggaca gcaaatttct tgtcagtcta ttgctgagtt
     1621 accacttgag caagtcaaag acacatttat gataaacatc atcgctatgt ttgctttagt
     1681 gaaagaagct gttccgcata tcccagcagg aggagcaatc gtaacaacaa cttctgttca
     1741 agcattcaat ccaagcgaac atttattaga ttacgcagca acaaaagctt caatcgctaa
     1801 ctttacgatc ggtttagcaa aacagcttgc acctaaagga atccgtgtaa atggtgtagc
     1861 acctggtcca atctggacac cattacaact agacgaagga caaccttctg attcgatccc
     1921 tgaatttggt cagcaaagtt tactagaacg tgcaggtcag ccaggagaat tagcaccagt
     1981 ttatgttttc ttagcatcga ataaagcaag ttatgttaca gcccaaattt acggagtaac
     2041 tggtggagaa gcgatcaact tataagaagt tgatcgtttc acccctatga ggaggtgcga
     2101 aaatgaataa aggtgtaaaa acgatcggag tgatcggaac attacttttg ctatctgtgc
     2161 ttttgtttgc gatactatta agtgcaacct atccattgcc ttttcagcta gagaagtttc
     2221 gattcttttc catcactaat tcatacgtgc aacaatatgt attttggctt gccgttgcat
     2281 ttgctatttt gacaattatc acgttacttg ttttcctgtt ttatccaaaa acaatcggaa
     2341 actttctact gaaacatggt gatggggagt tgacgttgga taaaaaagca attgaaggaa
     2401 tggttcgttc tcacttgcat gaagatgaat tcatccattc accaaaagta aatgtcaaag
     2461 caacgaaaaa caagatcgcc gtcaatgtaa aaggggagtt gaaacgaact tcagcactca
     2521 ttggtaaaac aggaacattg atgaaagaaa tcgaaaatga agtacaacac atattgggaa
     2581 caaaagagcc agtgaaagta tcggtgaaat acacaggctt tgaagaacca gctaaacgaa
     2641 gcaatgaccg caaacgagta gaataggggg gaccaaaaat gaaagaacta ttttcgacat
     2701 acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta tggatcaccg
     2761 tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc gctatcggtt
     2821 tttatctgaa acaaacgaaa atcttagatc actattttaa atcttaggag gaaacatcca
     2881 tgaataacaa taacaaaaat aataacaata aaatgaacaa ccaaaaacca gtgtctgcac
     2941 atccagaccc aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag aacaaaacag
     3001 caccagtatc tgcacatcca gatccagtag tccctaacgt tcaacaagga gtagaagaga
     3061 agaaacatga tgcagaaaag catgcacctc atcatccagg aactccagaa tctaaagttc
     3121 cagaacaaca tgatgcaatc gatccttcta aacctgtgga agagcaaaaa catccttctg
     3181 accatgcgaa agcacaagaa aaacttagtt ctgaccacac aaacggacaa ggaaaacttg
     3241 gttctgatca tacaaataac caaggaaagc atgattctga acaggaagat tcaatgcgcg
     3301 gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca attgaacaag
     3361 tagacggttt attatctgca aacggtggtt tcttctctaa tgtggcagga aaacttgtga
     3421 atacagataa tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa gtagcagttg
     3481 atatggatgt cattgtggaa tatggcaaag atatcgaaaa aatctttgaa gaaatgcaag
     3541 aagtaatcgg caaagaagta aaaaatatga cacatttaga agtgatcgaa gtcaacgcta
     3601 acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg gtgcaagaca
     3661 aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca gataaagtaa
     3721 aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa tacagaacca cgggttcagt
     3781 aataaggaag taaaacatta ggaggaacga aacatgggat ttatttggtc acttatcgtc
     3841 ggaggaatta tcggagcaat tgcaggagca atcacgaata aaggttcttc gatggggatt
     3901 attgcaaatg tcgtagcagg tttagttgga tcagcacttg gtcaagcatt attaggttct
     3961 tggggaccaa gtattgcagg aatggcaatc gtgccatcat tgattggtgc catcatttta
     4021 gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa agaggtcgtg
     4081 aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
     4141 tcatttggtt gggcatatta tcgaagatgc aagcttttga acactgttta tacggaataa
     4201 gaggctgtga caagactttt gtcacagcct cttatttatg cgggcaaatt cccaattaga
     4261 tttttaaaag tttttcctta aatgtgtctt tattgataca gagaagcttc tttaataaaa
     4321 caatgcctcc ctaaattttc ttgagatagg aaaggtgaac atacaaaata atacgaccac
     4381 tttgaaaacc cagatgatcc acaaatggat cgtatgatac tgatgaagta aaaagcaatg
     4441 gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa taatatctct tttttactct
     4501 tgtctgtttt agccaataat agctcgtaaa gtaatctaga caaacaaata gaagtaaaca
     4561 aagtttcgta agagcagcaa tcgtatcatg ttgctctgct agacgattga cgagcaggaa
     4621 aaatgttggt aatggaatat agatcacgag taatgaaaga aaaaagtaaa caataaatgg
     4681 catactattc ctcctaattg tgtgagttcg ccctcatttt aaaataacga tatttgtttg
     4741 tgaagctttt tcgtgtcgta acaaatgtta tctaatgtat ttttaaataa ataatcatta
     4801 ttatcaaaat aat
//
LOCUS       DACCSK010000156         4800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCSK010000156 DACCSK010000000
VERSION     DACCSK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.160"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..318
                     /locus_tag="IVP39_002558"
     CDS             <1..318
                     /locus_tag="IVP39_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587606.1"
                     /translation="KYGEYKNVLLSDEQMEKLKTEFPNDHQERIERLSEYCESSGKTY
                     KNYLATIRSWAKKEKSEPKNTSSGYKRTGRREKLPEWAIDQEAYQKKKALERANRQSK
                     APF"
     gene            330..599
                     /locus_tag="IVP39_002559"
     CDS             330..599
                     /locus_tag="IVP39_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587607.1"
                     /translation="MKIDYLELINEIASYKTGEEIEILRDVYEQLDEAGIERIKNDRS
                     SWSKLRYYFALYIDATQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            761..1063
                     /locus_tag="IVP39_002560"
     CDS             761..1063
                     /locus_tag="IVP39_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587608.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNENEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGNKLLVTGTAEQLASLTGLHEKIVRKRARCGYTDVKKRTFRYVEESS"
     gene            1060..1221
                     /locus_tag="IVP39_002561"
     CDS             1060..1221
                     /locus_tag="IVP39_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7587609.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIVNL
                     RREIRGLEE"
     gene            1218..1523
                     /locus_tag="IVP39_002562"
     CDS             1218..1523
                     /locus_tag="IVP39_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587610.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRRIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            1523..1840
                     /locus_tag="IVP39_002563"
     CDS             1523..1840
                     /locus_tag="IVP39_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7587611.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKSKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILEEYKE
                     DSK"
     gene            1837..2223
                     /locus_tag="IVP39_002564"
     CDS             1837..2223
                     /locus_tag="IVP39_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587612.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFDGDVVQAEQYLTTTIPVRINGIVKYSNRYTMFYLDNGSERHDLYM
                     QSLGGSIYNFEIIGNIYENPELLEEQ"
     gene            2223..2936
                     /locus_tag="IVP39_002565"
     CDS             2223..2936
                     /locus_tag="IVP39_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP7587613.1"
                     /translation="MNKQELIEKLAKYVKRYENVMDEHGQGRYCAYEVSLKLAKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGESQKPVMPKLFDDWAKRVIAKH
                     DEFYAISLVARAGWGYGVDFELSENGSSSENKELLYWLVDKCSDNYPNKKKAIEALLY
                     GYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAATERYGAKWKNTFTESEIK
                     AIDERYWPFAVPVEEEAEG"
     gene            2933..3310
                     /locus_tag="IVP39_002566"
     CDS             2933..3310
                     /locus_tag="IVP39_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587614.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            3307..3537
                     /locus_tag="IVP39_002567"
     CDS             3307..3537
                     /locus_tag="IVP39_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587615.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            3544..3747
                     /locus_tag="IVP39_002568"
     CDS             3544..3747
                     /locus_tag="IVP39_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7587616.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            3744..4040
                     /locus_tag="IVP39_002569"
     CDS             3744..4040
                     /locus_tag="IVP39_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587617.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWTAKSKENLAVARLVDMELINDK"
     gene            4116..4529
                     /locus_tag="IVP39_002570"
     CDS             4116..4529
                     /locus_tag="IVP39_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7587618.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
BASE COUNT         1766 a          742 c         1120 g         1172 t
ORIGIN      
        1 aaatacggag agtataaaaa tgttcttttg tcagatgagc agatggagaa actcaaaaca
       61 gaattcccta atgaccacca agagcgaatc gaacgactgt cagagtattg tgaatcatct
      121 ggtaaaactt ataaaaacta tttggcaact attcgaagct gggcaaaaaa agaaaaaagt
      181 gaacctaaga acacaagtag cggatacaag cgcacaggaa gacgagagaa gcttcctgaa
      241 tgggcaatcg accaagaagc ctatcaaaag aaaaaagcgc tagaacgagc taatagacaa
      301 tcaaaagcac cattctaaga ggtggaaaat tgaaaatcga ttatctagaa ctgatcaatg
      361 aaatagcaag ttacaaaact ggtgaggaaa tagagattct gagagacgtt tatgaacaac
      421 ttgatgaagc tggaatcgaa cgaattaaga atgatcgttc aagttggagt aaactcagat
      481 actatttcgc actttatatc gatgcaacac aattaagaaa tttagcttat accaaattac
      541 tatttgttga ttgtgttaaa ggcttgcaaa aacatcttaa tgaacttgag caggtgtaat
      601 cagatggacc taaagacatt tacagcacag attgaactaa tgcatcaaga agctttaaga
      661 caaagtgtat cgtacgaaga caagtggctc aacacgttcc acggcggacg tgagagcgca
      721 cttgatcaag tactcaaatt actgaaagga gaatgtcggg atggataaga aagcggcaat
      781 gcagcgaatt atcgaattga cttattcaga agattggcaa aatgaaaatg aagctgcttc
      841 agaagtgatg aggcttggaa gagcgatgtg ggcagacaag agcaacaagc caagaccacg
      901 aaaaatcgca atttggcacg gtaacaaact tctagtgaca gggacagctg aacagttagc
      961 aagtctcaca ggcttgcacg agaaaatcgt gagaaaaaga gctaggtgtg gatacacaga
     1021 cgttaagaag agaacgttta ggtacgtgga ggaatcatca tgacaacaga agaagtgatt
     1081 caaatgcgta ttcgaagcct tcaacgtgaa attgacgatc tggaacgaac aaaggcagtg
     1141 atggtcaatg aaacggcaag gaaggcaatc gatttgcaca tagtgaattt aagaagggaa
     1201 attcgtggat tggaggaatg agcgtggata agaaagcgac aatgaaacga attgctgaat
     1261 taaccaagtc agaatcttgg caggaagaca aagaaatagt tgcagaagtc caaaagctcg
     1321 gtaaatcaat gtggactgaa aagcccaaac ggaaaacgcc gagaagaatt gcaatctggc
     1381 atggtgatcg aattctagta acaggtactg ctgaacagtt atctgaaatt actggattaa
     1441 gcaaaaacat tatttgggat agagcaagga gcttatggat tgattcaaaa ggacgacaat
     1501 ttaggtatgt ggaggagaaa taatggatct cattacacaa tacagtgata ttatcctcaa
     1561 aaaaatcatg gcaaagattc agaaagataa aaaatcaaaa gaacgagctg aattagttaa
     1621 gttggaaatg gctgaaacag gagtaggagt gcgaagtagc aggcattgga aagcagcagc
     1681 aaacattgaa ttttattaca acgaaattca aaaagggttc gatcaaatgc gtgagctgga
     1741 tcggcaaaca aattggagca agaaacttca tcaagatcgt ttcaaatttg tagaaaagta
     1801 tagagagata ctagaggaat acaaggagga cagcaaatga taccgaagtt tagagcgtgg
     1861 tacacaccat ttaaaggtaa aacaattgga caagaaatga aatatgggca agcaggaagg
     1921 ttgatcactc atgctgaaat ggctccagat aaatatgtgc ttatgcaatc aacaggactg
     1981 aaagataaga acggtgtgga gatatttgat ggtgacgttg tgcaagccga acaatattta
     2041 acaacaacta tccctgttcg tataaacggc atagtcaagt attccaacag atatactatg
     2101 ttttatctag ataacggaag tgaaaggcat gatttatata tgcaatcatt aggaggtagc
     2161 atatataact ttgagatcat cggcaatatc tacgaaaatc cagagttatt ggaggaacag
     2221 tgatgaataa acaggaattg attgagaaat tagctaaata tgtaaagcgt tatgagaacg
     2281 ttatggatga gcatggccaa ggaaggtact gcgcttatga agtatcttta aagttggcga
     2341 aaagactaaa tgaatcaaaa attacagacg aacaagcttg gaataaggta gctgaggctt
     2401 atcctgaatc ggcacaaagc ttgagaaaca ctttagataa tgctgtattt ggtaagactg
     2461 gtgaatcgca gaaaccagtt atgcctaagc tttttgatga ttgggcaaaa cgagttatag
     2521 caaaacatga cgagttttat gctatttcac ttgttgcacg tgcaggttgg ggatatggtg
     2581 ttgattttga acttagcgag aacggatcat catcagaaaa caaagagctg ttgtactggc
     2641 ttgttgataa atgcagcgac aattatccta ataaaaagaa agcaatagaa gctttgttat
     2701 acggctacga ggtcgagaaa gaaccgttgt atcacgtgaa gctaaaaata ccaggcgtaa
     2761 catactacct tatccaaact tttttaatgc caggagatac tcatatgtgt ttctcagcgg
     2821 ctacagaacg ttatggggct aaatggaaaa atacttttac ggaatcagaa ataaaagcaa
     2881 ttgatgaaag atactggcca tttgctgtgc cagtggaaga ggaggcagaa ggatgaaaaa
     2941 attgcaccaa ctcatatctg agaaagaaag cgaactccaa aatttagaag atagtttggg
     3001 attggggttt ccaattgttg agcaagctaa aatgactcaa atcagtcatc tacgacttga
     3061 attggaagat ttaagacaaa tagaaaaatc aattcaactc aacgacaacc agcaaatcgt
     3121 gtttgagtgg ttgaaactaa ctgcgccaac aggaaagcca atgcaagttg tattttggat
     3181 gatgaataat gcagcatggg ggcatttaga cgaattaagg gatcctttga tggaattaac
     3241 agacaaagag caattcgaag tcctagcagc attcgctcaa tggggattag aacaggagga
     3301 agcggaatga aatacgaaat accactaagt gaagcagacg tccagtcaat tattaacggt
     3361 cgggaggtta ataaaaaact tcctgatggt actgaattag tcatcagaca aagttatttg
     3421 aaagatatgg cagctccagt attaattgat cgttttaacg tgactgattc tgtggtagag
     3481 aaccacttga aagaatttcg atcaagtata aacgacactt tcagattagg gagttgattg
     3541 acaatgaaca ccagacaccg cagagtagca aaactaagaa aacaggaact gaatgtacta
     3601 aagacaaagt ttgaaaaaga atatggaatt tcagcagaag aaacatataa agtggcaagt
     3661 cagtttgttg ctgatgcaag tgatgctatt cgtaagtttg ggatttcgat attaaatgat
     3721 gatcgtaaat gggaggaaat gagatgaaac taaaagacgg attttacgct agtagtcacg
     3781 gcatcggcgg tttaatgcta gatatgccga caaagaatcc taaaacacgt aagaaaccaa
     3841 aagtaaaagt tggtgacatg gttcgctgtg aagcagaagg gttcatctat ccatttcgtg
     3901 gatatgtaga gcatctctat aatcactcag cgatcattcg tattgaaaac acgatggaat
     3961 gtgataaatg gacagctaaa agcaaagaga atttagctgt agcgagattg gtggatatgg
     4021 aactaatcaa tgacaaataa aaaagccgga tcgctccgac tgttctaata aattccacaa
     4081 gtttattata tcacataaag gagcggtttg acttgatgca attgttacga gaggtagatt
     4141 tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt ttggagcgga
     4201 tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg ccgaaggcac
     4261 cgaagcatgg caataaggca gaagacgcga ttattcagat gatggatata gaaacggaga
     4321 gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag atactctact
     4381 acagcttctg tgtgccagat agcttctcaa actacagaat tagccgtgaa gtgggttatt
     4441 cagaaagaag tatacaacgg atgaagtcgg aagctctaat agagtttgca gaagcatata
     4501 aacacggaaa aataattgct tataaataat ttggcggttt tttggcggaa tgatggcggt
     4561 ttttagacat ttaccagtgt tattatggta gtgtcgaaag attaggaaac aggatcgaca
     4621 aaataaacga agggaggcaa tctccctcat cgttttaaac ttcttcttag atagacagca
     4681 actaattatg aagaaaagga tgtgaattca actcctccta aattcttcat cggttgctgt
     4741 ttattttgat tagacagcag cactcacaaa actaaacatg ttcttgtcgt ctttcaactg
//
LOCUS       DACCSK010000157         4759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCSK010000157 DACCSK010000000
VERSION     DACCSK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.161"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            40..711
                     /locus_tag="IVP39_002571"
     CDS             40..711
                     /locus_tag="IVP39_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7587619.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            770..1210
                     /locus_tag="IVP39_002572"
     CDS             770..1210
                     /locus_tag="IVP39_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587620.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1222..1890
                     /locus_tag="IVP39_002573"
     CDS             1222..1890
                     /locus_tag="IVP39_002573"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7587621.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1954..2577
                     /locus_tag="IVP39_002574"
     CDS             1954..2577
                     /locus_tag="IVP39_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7587622.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2633..3019
                     /locus_tag="IVP39_002575"
     CDS             2633..3019
                     /locus_tag="IVP39_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7587623.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3232..4623
                     /locus_tag="IVP39_002576"
     CDS             3232..4623
                     /locus_tag="IVP39_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7587624.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1586 a          783 c         1012 g         1378 t
ORIGIN      
        1 atatgctatt ttaaaaatga tgaaaaaagg gggagaaaaa tgaatttttt agttgtttta
       61 aaagatactc tgattgagcg atctacattt ctatatgttc aattgcagga aaataaaaca
      121 ttgctgcatt tttcaccaga gttccatcta gaggggctaa cattaggaga aatctatcaa
      181 gcagaagggc tgtcggctgt tttgcacttt tttaaggtag aattggaatt accagtaaaa
      241 gattatatcg agctttctct tgaatcttgg gatcgagcag tggttgaact gtttccagaa
      301 ggtttaatga tcgatacaaa cgatgggaag atccatctga ctgcagcaga actgcaaaaa
      361 caaatgcgtt atcaaataga tgacgaaaat tctttttcta tttttagacg tcagcaaaag
      421 attctaaaaa gctttttaaa agtgatcagt aaaaaacgga atctgatcaa aacgacacag
      481 ctgttgaaaa aatatccagg tctgcttcac acgtctattc aatttcctca attgattacg
      541 atgatccatc gttttatacg tatgcaacag ctaccatcta agaagctgtt tcttccattg
      601 acggacacat ttcgagtagt caacagagaa gataaaaaga aagtgattgt gattgacttt
      661 accagaaatc ggaatgtcct tcaccaagtt gcaatggaaa aggccaatta aggctttttc
      721 tttttggatt tttttgtcat aataaatatt agatgatgga ggagataata tgatgcaaac
      781 atggttagaa aggctaatac gatttcagca gaactatcac aaacttatgc agggaagata
      841 tgcgaaattt gatcagttga atcgcggtct attgatcgct gcgcttgtct ttagtttgct
      901 aagtcgttgg ttgccctata gaatcggaca attgttgttc atcgtctttt tcggatggat
      961 gatatttcgt ttcctatcaa aaaaaatcta ccccagacta aatgagaatc aacgttattt
     1021 gaaatatctt gatagagtca aagaaaaata tcatcagata aaaaacaaaa agcaagtcaa
     1081 aatggatcga aaaagttata ctttttttga gtgcccgaat tgtcatcaaa agcagcgtgc
     1141 acctaaagga aaaggacgaa tacgtgtgac atgcaaaaca tgtggtataa aattcgaaac
     1201 gaatgtttaa gggagaaaag aatgaaatta gtattattac gtcatggcga aagcgaagca
     1261 aattttgaaa attactggac aggatggcta gatgtcgcct taacagaaaa aggaaaagaa
     1321 caagcagcag ttgccggtca aaaaatgctt gaagccggac ttcaattcga tgccgtttat
     1381 acctctgttt tgaaacgtgc gattcttacc ggacaagtcg cactagaaga aatgggtcag
     1441 ttatggcttc ctataataaa aacatggcgt ctaaatgaac ggcattatgg ggatcttgtg
     1501 ggtaaaaaca aagataagat gaaagaaata tacggaaaag atcaagtaaa aacatggcgc
     1561 agaggatata cggaagttcc gccgttaacc gcagaaaata gatttgaccg cagatatgat
     1621 cacttggatc cgcatttgat cccatacgga gaaagtctgg aaatgacagt gaatcggatc
     1681 attcccttat ggcaagatca tctagcacca aaactaaaag atcggcaaga tttactagtt
     1741 gtgggtcatg gtaatagcat ccgagcactg acaaaatatt tagaagatgt tcctgaagat
     1801 caaatggata cgatcgatat cccaaatgca caaccaatcc agtatacatt agcagatgat
     1861 ttgacaattc tggacaaaaa aatactttaa agaacacctt tatctaaaag tgagagatag
     1921 aaaaaagaag caaaggaatg gaacaaaagg aacatgaaaa attatatatg ggactttgat
     1981 ggtacattgt ttgatacgta tcccgcaatg gtagatggtg catggcaagc actaaaagat
     2041 tttggtatca gtatggacaa aaaagaaatc tacttcaaaa tgaaaaagta ttctactagt
     2101 tatttgatca atgaatcaaa cttgaatgcc agagagttta atgaattatt tcaccgatat
     2161 gaaaaagaaa gtacggaagt ttcccgtcca tttccagaaa cgaaacaagt actggaaatg
     2221 ctaaaagaca acggcggtcg tcatttcatc ctgacccacc gtttgacaga atcaacatgg
     2281 ggcctgttaa aagaacatcg attggcacat ctgattgagg aagtagtagg gatcgatcag
     2341 gactttcctc gtaaacctga tccagcatct ttaaattatt tgatcgatac ttttcattta
     2401 gaaagaacag acacgatgat gatcggcgat cgtcgtttag atatcgaagc agggaaaaat
     2461 gcaggagtcg ctacttgtct ttacgacatc gatcattttc tgggtgagat cccggcagat
     2521 tatgtggtag gaaatctgaa tgaaatcctt accttgcaga gcatcattaa aaagtagtat
     2581 cccctttcaa aggagatatg ttataatttt cataaaggag tgataaaatt atatggaaat
     2641 cagaagacaa aaagaagttg tagaagcatt tcattatgat atgagattgc cgaaccaaga
     2701 agtagaaaca gatttgaaag taggcttctc gcctttaaaa gaaacgatcg aaaactatcc
     2761 gaaagaacat agcatcgtag gagcacgctt agagttccgt ttagtgtttg atacatatgt
     2821 tttatctgga agtgtgagtc agatcaatca tatcctcaat cggaaaattg aaggtcaaaa
     2881 tgatgtcaca caagaagaag tggacgagct agtagcaccg ttattcgata tcgtacgccg
     2941 aatgtcttat gaggtgacag agatcgcctt agatcagcct ggggtaaaat tgaattttca
     3001 atcagaaaaa caagaataga cagtaaaaac atacctgcga tagattaaag ctatcacagg
     3061 tatgtttttt tattactaat tgtaagcaaa aagaatgtca tttcgataaa aatcgtctaa
     3121 aatataaaag tcactgaaaa aaattttttc aggaggatgg ttgaagaatc attctcggga
     3181 acctttactt ccttcgatgg acttgtcatt aataatatga ggaggagaaa aatgacaaca
     3241 ctcatcacag ttttagtggt tctagttttt gcggcattat tatatgtgtt ctatcgtatg
     3301 caaaagcgcc atacaaaatt ttcaacacgc gtctttacag cattaggagt tggtatcgtc
     3361 tttggcggtc tgcttcaatt gatctttggt gtagacgaca aagtcgttac tcaatcaatg
     3421 gaatggatcg ggattgtagg tcaaggatat gtatcattct tgcaaatgct agttatgcct
     3481 ttagtgttta tttcgatcat tggtgcattc acaaaaatga aagtttctga aaagctaggg
     3541 aaaattagtg cgactgtatt gacttcttta cttggaacaa cagctattgc agcattgatc
     3601 ggaattggtt caacaatgct gttcggactc caaggagcta aattcacaca aggagctgca
     3661 gaaacagcac gaatctctga actggcaaca cgtcaggaaa cagttgaaaa cttatcgatt
     3721 ccacagcaga ttttatcctt tatccctaaa aacgtttttg ctgatttagc aggtagtcgt
     3781 gcaacaagta cgattggagt cgttattttc gcggcatttg tcggaatcgc ttatctaggc
     3841 gtgaaaagaa aagcgccaaa agaagcagca ttcttttcag atttagtcga cagtctgtat
     3901 aaaatcatta tgcggatcgt gacgcttgtt ttgcgtttga caccttatgg agtatttgct
     3961 ttgatgacaa acgttttggc aacaagtgat tttgcagcta tcgtcaattt aggtaaattt
     4021 gtcgttgctt cttacgtagc attgcttgct gttttgattg tccactcact gattttgatt
     4081 ggtgcaaaag tcaatccaat caactatttc aaaaaagcct ttccggtatt gagctttgcg
     4141 tttacttcac gttcaagtgc tggagctttg ccattgaata tcgagactca gacaaaagct
     4201 ttaggagtag atgatgcaac agctaatttc tcagcaagtt ttggtttgtc tattggacaa
     4261 aacggctgtg ctggcgttta tccagctatg ttggcaacga tcgtcgcacc agcagctgga
     4321 gtgaatgtct ttagtttgga atttattttg atgcttgtgg caattgtaac aatcagttct
     4381 tttggtgtag ctggagtcgg tgggggcgca acatttgctt cactgatcgt tttaggggca
     4441 atgaacttgc cagtagcaat cgttgggtta gttatctctg ttgaaccatt aatcgatatg
     4501 gcacgtacag cagtaaacgt cagcggtagc atggtagctg gtatcgtgac aagtgcacgc
     4561 atcaaagatt tagatcgcga tgtcatcaat gatgattcta aagtgatcga tgtgaatgca
     4621 tagtcaaaaa gaggttgtga tagaagtggt cagctttaag aaataagaag aagtttccta
     4681 aaaatagttt ctcaaatttt tgtgaaattt cggcttattt ccgagaagct acttctgaaa
     4741 caccgtgaat aagaggttg
//
LOCUS       DACCSK010000158         4759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCSK010000158 DACCSK010000000
VERSION     DACCSK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.162"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            314..2158
                     /locus_tag="IVP39_002577"
     CDS             314..2158
                     /locus_tag="IVP39_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7587625.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSSTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2213..3679
                     /locus_tag="IVP39_002578"
     CDS             2213..3679
                     /locus_tag="IVP39_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7587626.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3716..4558
                     /locus_tag="IVP39_002579"
     CDS             3716..4558
                     /locus_tag="IVP39_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7587627.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1555 a          765 c         1021 g         1418 t
ORIGIN      
        1 ttggaagata ttcttagaaa aaagacaaaa cttttagaac acgaaaacta tattgaacga
       61 ataaaatgaa aaaatgatta aaaaatcatt ttattattta cgtttacatt ctaatatgtt
      121 aaattgaata taacttaaac aggcatgtga ctggtaatgc aggcaggacc taaataagtt
      181 gaataacaat gtatgcgttt caatacacgt ttgccttgtt gtcaatttat ttagtttctg
      241 cctgtttttt gtgttgagga tggccacttc gactcaaaag ttcatcactc aaaaaagaaa
      301 agagggaaac accatgaatt acaaacaaac agcagaagaa gtattgcagt acatcggtgg
      361 ggaagaaaac atatcccatt tggagcattg ctcaactcgt ttacgtttta cgttaaaaga
      421 caacagcaag gtaaatatga gccagttaga aaaagtggat ggcgttatgg gcgttcgcca
      481 aaatgtgcaa tgccaagtaa tcattggaaa cgatgttgtt gaagtgtatg atgaagtgaa
      541 agcgttatta ggtgacaatg tttcaagtac tgaagaagca ccaaaacaaa aacaaaaatt
      601 aggttcagtt ctattagatt ttattatttc tattttccaa cctttaattc ctgcgattgc
      661 aggtggagga gtattgaagt ccttgttgct actagcttct atcttagggc tgattagtga
      721 ccaatctcaa acctatcaga ttttaaattt ggtcggcggg gcaccgcttt atttcttgcc
      781 tattctggtg gcaatgacta cggctaataa attgaaagtc aatcaattgg ttgcagtatc
      841 tgctgtcggt gcattgatcc ttcctgaaat gacagctatg ttggcggaag gtgctacatt
      901 cttatctttt ggattggata atatagcgta tgcatctcaa gtattcccag ccatcttaac
      961 tgttttattt tatgctcaac ttgaaaaatg gtttacaaaa gtttcaccaa aaccaattcg
     1021 tattttcttt gtacctatga tgagtttatt gattacagtt cctattgctt tgttgcttct
     1081 aggaccatta ggttataatg taggaacagc tttctcttct atcattgtgt ctttgtacaa
     1141 tcaatttgga tgggttgcaa ccggagtctt agcagctgta ctgccattaa tggttgtaac
     1201 gggtatgcat aaagcaatga tcccttacgc tgtttcatcc atgagtgaat tgggtgcaga
     1261 acttctttat ctcccggctt cattggcaca caatatttca gaagctggcg catgctttgc
     1321 tgtcagtatc aaaacaaaag acaaaaaatt gaaatctacg gctgtttctg cgggtatatc
     1381 tgcattattc ggaattactg aaccagctct ttacggggta acgattctgc acaaacgtgt
     1441 tttatatgcc gttatgtttt ctagcctgat cgggggagca tttgcaggaa tcgttgcact
     1501 tcaagccttt gctttagtgg gtcctgggat tgcaagtatc acgatgttcg ctgacaaagc
     1561 tgacccaatg aatctggttt gggcatttgc agtatcgggg atttcatttg tggttgcttt
     1621 tgctgcagcg ttggttcttt ataaagataa agaagttgtc aatgaagttg aaactaagat
     1681 ggaaacggcc gttgtttcag atgctgttct ttcaagtccc gtgaatggaa aagtaatctc
     1741 actatcagaa gtaaaagaca ctgttttttc tagtggtatg gtaggtgatg gagtagcaat
     1801 cattcctgaa gaagggatat tagtagctcc tgaagatgga gaaatcacaa tggtatttga
     1861 aacgaagcat gctattggtt taaaaacggc taatggtgca gaacttcttt ttcatattgg
     1921 tattgatact gttcagttag aaggaaaagg gtttgaggca tttgttcgtt ctggtgaaaa
     1981 agtagccaaa ggacaaaagc tgattgcatt tgatattgat ttaatcaaag aagcaggatt
     2041 cgaccccaca gttatctgtg tggtaaccaa taaggataat tacactatcc gttcatttaa
     2101 cctagaaaaa ttggtaacca atgatagtga tctcttagct atcagtccga tagcttaaac
     2161 acaagatagt gaaaaaaata tattgaatga aggaatagag gagaatagaa taatgacaaa
     2221 cacaacaagt tttccaaaag gatttttatg gggcggcgcg atagcagcaa atcaagtaga
     2281 aggtgcttgg aatgtagacg gtaaaggtct ttcagtagcg gatatggcta cttataaacc
     2341 aaatttagac aacaaagatt ttgctgctca tatgcatgtc accctgcagt ctataaaaga
     2401 agctagtgaa gacaaatctg ataaatggta cccaaaaaga aggggcgttg atttttatca
     2461 tcactataaa gaagatttag ctttatttgc cgaaatgggc tttaaaacct tgcgcctctc
     2521 tattgcttgg actcgcttgt tccctacagg agaagagctg gaaccgaatg aaaaaggggt
     2581 tgcattttat gaaaacgtct ttaaagaaat gcaacgttta ggaattgagc cgattgtgac
     2641 actatctcat tatgaaatgc cattagcttt agcagttaaa tataatggct gggtggagcg
     2701 tcgagtaatt gatgattttg ttcgcttcgc taacgtttgt tttgatcgct tcggcaaata
     2761 cgtaaaatat tggctgacat ttaatgagat agacagcatc caccgtcatc catttacaac
     2821 agctggaatt atccaagaga aaagcgcaga aggtcaagaa gctcaagata tctatcaagc
     2881 attgcatcat caatttgttg cgagtgcgtt agtgacaaaa gatgctcatg aaaaaattcc
     2941 aggaagccaa gttggttgta tgttaacgaa attgatgact tatccattaa cttgcgcacc
     3001 agaagacgtt gagctgacct tgaaaaagaa cttagaaaat aatttttatg cagatgttca
     3061 agttaaggga gaatatcctg ctctaatcga aaaggatttg gaaaatcggg gaattcagat
     3121 taaaatggaa gatgaagatc gagaaatttt aaagaaacat accgtagatt ttgtttcttt
     3181 cagctattac atgtccatgg cagaatcagc tgatccaaac gcagaacgca caccgggaaa
     3241 tactgtttta ggggttaaga atcctcattt gccttcaaca gattggggct ggcaaatcga
     3301 tccaaaaggt ttgaaaattt cgttgatcga actttatgat cgttaccaag taccgttgat
     3361 gattgtggaa aatggtatgg gagcagtcga tcaggtagta gatggtaaaa tccatgatcc
     3421 gtaccgcatc gactacttta aagcacactt ccaacaaatg aaagaagcaa tcgatgaagg
     3481 tgtagatttg atagcttata ctagctgggg accaattgat ttagttagtg caggaacttc
     3541 tcaaatgtct aaacgatatg gatttattta tgtggatgca gacgatgaag gaaatgggac
     3601 atataaccgc ttgaaaaagg attcgttcaa ctggtacaaa gaagtgatcg aaacaaacgg
     3661 tgcatcctta ttcgagtagt ctcgatcttt ttaagcagcg atagggggat aatgagtgat
     3721 ttcaataaaa aaagtattga attcaagtgt ggtactggtt gaagaaaatg gcaaagaaat
     3781 gattgcatta ggtaaaggga taggctttgg acagaaagct ggggatgaaa tatcaggaga
     3841 taaagtagat aaaatatttc tgccttttga agaaaagaaa tcttcacaac tattagagtt
     3901 agttgaagaa atacctattg aattttttga aatcactcgt actatcgttt ttgaagcaga
     3961 aaaaggatta gataaaaaac tgaatcctgg agtctattta accttaacag accacctgca
     4021 ttttgcagtt gaacgcgcac aaaaaggggt aaatattgca aaccgtttgt actgggaaat
     4081 aaaaatctac tatcccaaag aatttcaaat tgggaagtac gcactcaccc ttttgaacga
     4141 gcaatatgga attgatcttc ctaaagagga agcctcaaac attgctttcc atttgatcaa
     4201 tgcacaatcg gaagatcctg atagcggcgc tggattcaag tatgcaaaaa tgatcggcgg
     4261 gatcgtcaac ttggtccggt attcgttgca gaaagatatc gatactaatt cgatccatta
     4321 tgcccgcttc attacacatg tacgtttctt tgtagaaaga tattattctg atgctttgtt
     4381 ggatgatggt gaggaagaat tctacaatca aatgtggatc ttatacccgt ctgccatgga
     4441 aagcgcagct aagatcaaag actacattga aaaagtttat cataaaacaa ttccgaacga
     4501 tgaagttgta tacctaggtg tccacatcaa tcggttgatg aaacatgcta atgggtgagc
     4561 gctcagtaat actattgatg cttttaaaat ctttagagat ggattatagt aaaaacaagg
     4621 tgatttccag ctgtttgtta tctataagag acatttaaaa aggattaacg taagataata
     4681 aaaaatctcg gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa taatttggac
     4741 agaacagaaa gaagaaaac
//
LOCUS       DACCSK010000159         4658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCSK010000159 DACCSK010000000
VERSION     DACCSK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.163"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            5..862
                     /locus_tag="IVP39_002580"
     CDS             5..862
                     /locus_tag="IVP39_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7587628.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            884..1393
                     /locus_tag="IVP39_002581"
     CDS             884..1393
                     /locus_tag="IVP39_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7587629.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1430..1831
                     /locus_tag="IVP39_002582"
     CDS             1430..1831
                     /locus_tag="IVP39_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587630.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1943..2296
                     /locus_tag="IVP39_002583"
     CDS             1943..2296
                     /locus_tag="IVP39_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587631.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2330..2773
                     /locus_tag="IVP39_002584"
     CDS             2330..2773
                     /locus_tag="IVP39_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7587632.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2790..3875
                     /gene="uxuA"
                     /locus_tag="IVP39_002585"
     CDS             2790..3875
                     /gene="uxuA"
                     /locus_tag="IVP39_002585"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7587633.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3868..4581
                     /locus_tag="IVP39_002586"
     CDS             3868..4581
                     /locus_tag="IVP39_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7587634.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         1613 a          709 c         1058 g         1278 t
ORIGIN      
        1 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
       61 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
      121 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
      181 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
      241 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
      301 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
      361 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
      421 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
      481 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
      541 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
      601 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
      661 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
      721 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
      781 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
      841 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
      901 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
      961 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     1021 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     1081 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     1141 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     1201 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     1261 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     1321 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     1381 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     1441 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     1501 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     1561 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     1621 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     1681 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     1741 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     1801 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     1861 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     1921 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     1981 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     2041 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     2101 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     2161 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     2221 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     2281 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     2341 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     2401 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     2461 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     2521 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     2581 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
     2641 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
     2701 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
     2761 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
     2821 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
     2881 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
     2941 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
     3001 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
     3061 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
     3121 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
     3181 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
     3241 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
     3301 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
     3361 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
     3421 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
     3481 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
     3541 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
     3601 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
     3661 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
     3721 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
     3781 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
     3841 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
     3901 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
     3961 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
     4021 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
     4081 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
     4141 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
     4201 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
     4261 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
     4321 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
     4381 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
     4441 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
     4501 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
     4561 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
     4621 tgcacgactg agggaacaga tgaaaaagaa gtaatttt
//
LOCUS       DACCSK010000160         4641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCSK010000160 DACCSK010000000
VERSION     DACCSK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.164"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(12..332)
                     /locus_tag="IVP39_002587"
     CDS             complement(12..332)
                     /locus_tag="IVP39_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7587635.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            806..1492
                     /locus_tag="IVP39_002588"
     CDS             806..1492
                     /locus_tag="IVP39_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7587636.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            1494..2438
                     /locus_tag="IVP39_002589"
     CDS             1494..2438
                     /locus_tag="IVP39_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7587637.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            2637..3281
                     /gene="pcp"
                     /locus_tag="IVP39_002590"
     CDS             2637..3281
                     /gene="pcp"
                     /locus_tag="IVP39_002590"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7587638.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            3360..4637
                     /locus_tag="IVP39_002591"
     CDS             3360..4637
                     /locus_tag="IVP39_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587639.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1525 a          820 c          966 g         1330 t
ORIGIN      
        1 attccaagtg tctagtggcc gctagtaatc ttgatgccaa tgatactgat gagtagcatc
       61 cccacaaaga accaagtgga aagattcagc ttatcatgaa acaagaccac accaattatg
      121 accgaaccaa ctgcgccaat ccctgtccag ataggataag agagactcat cggcaaactt
      181 ttgattgcct ttgacaaaaa gtaaaaacta gcaatcatgc caatcactgt gtaaaaagaa
      241 taattgattt tagaaaaacc atcactccat ttcatcatcg ttgaccagaa tacttccaaa
      301 attcctgcta tgattaattc catccatgcc atacttacat tcctccttaa ctataaaaaa
      361 atacacaaaa agaggaaaat catcccttca aagacctaat ggaatgatca tcctctttat
      421 cctacccggt ggcagattgt atatcttttt ctgtagtata gcacacctat ccagatatcg
      481 gcaatctcat actttttagt aaagtgtacc aagcactgac ggatatatcg ttagcggaaa
      541 agaagatgaa tgctttgcaa tttcatagaa agaagttgtt atttattaat catttaaaaa
      601 aaacagtaac tagaccttat ttctatatat tatcgggcaa taaattaaaa aaagctttac
      661 cttacaataa gtaagataat ttttgtgata aaaaaataac gaaaaatatt taaaaatagt
      721 gtctatttcg tgagaaaaca actatatttt ttatgtgttt tgctttataa ttaaaaaaat
      781 attgtcattt tatggaggaa aatatatgga aaattatctt agccttttag gcgtcttgat
      841 tgttattctc gggtttgcat ttaaattaga ctcgatattg atcgtaatgg ttgctttagt
      901 tgttacagct ttgactggcg gtcttggcat tcaaggaatg ttagaacttt taggtacaag
      961 ttttgtaaac aatcgcggga tggcgatttt tatcatcatt atgttggcaa caggaacgct
     1021 tgaaagaaat ggtctgaaag aatcagcggc ttccttgatc aagcgattca agaaagtatc
     1081 tgctggtatg attgtagata tttatgggat ctttcgaatg atctttgcgg cttttaacgt
     1141 gagttttggt ggagttgctg gatttgttcg tccaatcatt ttaccgatgg cattggggac
     1201 catcgaatca aaagggttag aagtccatcc taagcatgaa gaagaactaa aaggtatggc
     1261 ttcagctatg gaaaatgtct gctggttctt tggccaagta ctattcatcg gaggagcagg
     1321 ggcgttgcta gtccaatcaa cactgaaaga tctaggatat gacgtaacat tgggacagct
     1381 tgctttagtg gaagttccag tggctatcac ggctttagtt gtggcaagtg tctacttcta
     1441 tcttaaagaa aagaaattag cgaagaaata ttatccagga aaggggcaat aagatggact
     1501 tttttacaaa tcctgatatt ttattaggcg ataaattact ggagatcctt tatatcgtga
     1561 tgggactcgt attgatttac actggagtaa gaaatttaac agataaaacc aatcctcatc
     1621 gttatgggtc agcctttttc tggacggtct taggggtagt tattgcagga ggaagatggc
     1681 ttcccgcaat cgtgaacggt ggcttgatct ttgcgatgac cataccagca attgctaaga
     1741 aagtcagcaa aggagaaagc cggcttccat caaaagaata tatggaaaaa atgtcagaca
     1801 aactaggaat gaaaattttt attccagctt tgagtatcgg tgtctttgcg atcttgtttg
     1861 ctctatttac caatttaggt gctttagtcg gcgtgggcgt cggtgttttt gcagccatgc
     1921 taatcttgat gttcttttct cgagataatc gaccaagtac atttttagat gatgcagcag
     1981 atatgctggg cacagtaggg ccgttaagca tgttgccaat gcttttagct tctctaggtg
     2041 cggtatttac aagcgctgga gttgggacag tgatttccaa tgcagtaggt acgatcgttc
     2101 cagaaggaaa tgtgaacatc ggcatcatca tttacgctct tggcatggtg atatttacgg
     2161 taatcatggg gaatgcattt gcggccatca ccgtgatgac ggttgggatc ggtgcaccat
     2221 tcgtatttag tcatggagcg aatccagcgt taataggaat ggttgcattg acttgcggtt
     2281 tttgtgggac cctgctgaca ccgatggctg ctaacttcaa tatcgtgcct gtagcgatgc
     2341 tggagatgaa agacaaatac ggagtgatca aaaaccagtt gtttattgcg ttatttatgc
     2401 tagttttcca aattgcttat atgattctat ttaaataata gaagagaggg gttatgacag
     2461 aataattgtc gcaacctctt ctaacattgt agtggaacac tattttttta taagaatata
     2521 ttaagtttga tctgttatgt tatttaaact attgtttcct ttatttacgc tgttcaagga
     2581 aaatggtatg ataaaagata accatatgtt aaatcaatcg gacggaggaa caacagatga
     2641 aagtactagt aacaggtttt gatccatttg gtggggacaa agtgaatcca gcctatgaag
     2701 cagtaaaaaa aatgccagat gaaatcagcg gtgctgaaat catcaaagtg gaagtaccga
     2761 ccgtattcga aaaaagcagt caagtagtca aagaagccat ccaacagcac caaccagatg
     2821 tcgtgatctg tgtcggacaa gcaggtgggc ggtcagcagt ctcgttcgaa cgtgtcgcaa
     2881 tcaatctagc agaagcaaga atccctgata atgaaggaaa tcagccattc gatacggctt
     2941 tagaagaaaa cggtccagcc gcttacttta cctcattgcc aatcaaagca atgacaaaaa
     3001 atgtacaaga tcatggtcta ccagcgtaca tctcttatac agcaggaaca tttgtctgca
     3061 atgatatcat gtatcgtttg cttcatttga tcgaaacaca attcccggca attcgaggtg
     3121 ggttcatcca cgtgccattc tcacccgatc aagtgatcga tcgtccagtg ggtacgcctt
     3181 ctatgtcttt agaagacatt gcttcttctt tgacttatgc agttgaagca gcagtagaaa
     3241 ataaagaaga tatttccgga aatgccggaa caactcacta atttagcaag aaacactggc
     3301 tggttgctaa cgttatgtta gtagccagct ttttgtaatt agaaaggaaa gaaacagccg
     3361 tggaagaatg gcaatccgtc tttgaagaat ggtttccaaa agaaatcagc aaaagctatc
     3421 ccatcaaaat ttctaaacaa tacacaagca gtcaacgttg ggagatctat gcaaaattaa
     3481 cgaaaaaaca gcgggaacta gtcgataagc accgccgata tctaatcagc tctcgtttta
     3541 tggaagaaca ttatttagct gcaacagatt gggtgttttc tgacttcaag atcaatccgt
     3601 tttttcgaac aaaacgaagc cagcaaaaat tgtattgtga gtgtggtaga gaattaaaag
     3661 tgcaatacat cgttaaatcg ccaaaaaccg gaaagatcct aaaactaggc atcaaccact
     3721 ttgccgatca cctgcatgtc tctccgacag tggcagcttc gatccatcag ggaatgacaa
     3781 aagttgatct ggccttagac gaattattat ggcttaagca gaagaatatc gattttcctg
     3841 aaggactatg gcaaaaatac tgttttgtct tgtaccaaaa tcgtcggatg aaacagccat
     3901 atttaccgga tataaaatta gctcagcggc tcgcagaatt tcgccaagta gaaatgccga
     3961 tttacatagc ggattatcaa gcattggaaa atgaaatcaa gaaaataagt gaacacatca
     4021 atgggcagtc aaaaaagcga cagataaaaa aagaactatt tgacgacttt gcagaagaat
     4081 tagtaaagga tgtagaagaa ttcttgatca actatcgcgc atttttacgt aaagattggc
     4141 aatccatcgt atatgaagaa gtgccagtac atcctaatgc ttattttgaa acatttattt
     4201 ccgtgttgag aaagacaaaa cgtcaacgaa caccagaagt cactgctcag atggaatatt
     4261 ttgcgaaaaa ccaacgattc atccaaccaa aaatctatct atttatttgg aagcaatatt
     4321 gtcgctatgg ttttacagaa gggttcttcg acagcattcc tcggatagtc agaaatggtt
     4381 ttttaaaagt attgcgtaaa gaacgagaag ctatacaatc tgctgataag aaagaccgta
     4441 cagtctcaaa agaaaaatgg caactagtag tgaaagatat ccaatctgga aatgtgcaag
     4501 agacaatcga caaatggaaa ggaaaacatt atcgttttac tgaagctcag aaacaagcgt
     4561 tggaatatta tcagaagtta gaagaatcac tgcgtttcaa tgatgaggca agaaagtatc
     4621 tgaaggaatt actataaaaa a
//
LOCUS       DACCSK010000161         4587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCSK010000161 DACCSK010000000
VERSION     DACCSK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.165"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(26..280)
                     /locus_tag="IVP39_002592"
     CDS             complement(26..280)
                     /locus_tag="IVP39_002592"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7587640.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(391..645)
                     /locus_tag="IVP39_002593"
     CDS             complement(391..645)
                     /locus_tag="IVP39_002593"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7587641.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(838..1113)
                     /locus_tag="IVP39_002594"
     CDS             complement(838..1113)
                     /locus_tag="IVP39_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7587642.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1085..2038)
                     /locus_tag="IVP39_002595"
     CDS             complement(1085..2038)
                     /locus_tag="IVP39_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7587643.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2650..4143
                     /locus_tag="IVP39_002596"
     CDS             2650..4143
                     /locus_tag="IVP39_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7587644.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4278..4574
                     /locus_tag="IVP39_002597"
     CDS             4278..4574
                     /locus_tag="IVP39_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7587645.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
BASE COUNT         1491 a          765 c          799 g         1532 t
ORIGIN      
        1 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
       61 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      121 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      181 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      241 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      301 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      361 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      421 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      481 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      541 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      601 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      661 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      721 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      781 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      841 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      901 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
      961 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1021 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1081 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1141 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1201 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1261 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1321 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1381 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1441 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1501 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1561 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1621 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1681 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1741 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1801 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1861 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1921 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     1981 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2041 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2101 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2161 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2221 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2281 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2341 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2401 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2461 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2521 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2581 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2641 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2701 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2761 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2821 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2881 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     2941 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3001 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3061 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3121 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3181 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3241 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3301 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3361 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3421 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3481 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3541 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3601 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3661 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3721 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3781 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3841 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3901 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     3961 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4021 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4081 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4141 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4201 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4261 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4321 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4381 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4441 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4501 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga aaaaggaaga
     4561 agtatatcca gtagtagttt ttattta
//
LOCUS       DACCSK010000162         4533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCSK010000162 DACCSK010000000
VERSION     DACCSK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.166"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            349..579
                     /locus_tag="IVP39_002598"
     CDS             349..579
                     /locus_tag="IVP39_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7587646.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            676..1977
                     /gene="murA"
                     /locus_tag="IVP39_002599"
     CDS             676..1977
                     /gene="murA"
                     /locus_tag="IVP39_002599"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7587647.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1990..2166
                     /locus_tag="IVP39_002600"
     CDS             1990..2166
                     /locus_tag="IVP39_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7587648.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2301..2597
                     /gene="yidD"
                     /locus_tag="IVP39_002601"
     CDS             2301..2597
                     /gene="yidD"
                     /locus_tag="IVP39_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7587649.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2661..3044
                     /locus_tag="IVP39_002602"
     CDS             2661..3044
                     /locus_tag="IVP39_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7587650.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            3213..4097
                     /locus_tag="IVP39_002603"
     CDS             3213..4097
                     /locus_tag="IVP39_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7587651.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4214..4447
                     /locus_tag="IVP39_002604"
     CDS             4214..4447
                     /locus_tag="IVP39_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7587652.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1534 a          693 c          990 g         1316 t
ORIGIN      
        1 tcagcttatt tttcgaagaa gctacttctg gaacaccgta tatcagaggt tgtgaaatca
       61 gcgttttgtc ctgagtatta gagaaacaaa tggaaaatag ttcctcatat tttgccgttt
      121 tttgagctaa taccgagggt cagatgattg aacaccgttt atttggaata agggactgcg
      181 acaagacttt tgtctcagtc cctttttttg catattttta gcatttattt cctaaaaaat
      241 gtaaggggat tcctatattt ttaagtatac ttgtttttcc ttttatacat tttatggtat
      301 tatcatttta ttagttatta taaaacaatc gttagaaagg acgtcattat gcaagtatac
      361 ggaattgatg cggtgcttcg aatcgtgagt catttggcgt ttatctactt agccttctgg
      421 tctttacgtt cgttacgcat tgaaactttt ttcagaccaa tgcataatac gcaaattaga
      481 atggcaatcg tacttttttc aattttttta gggtacactg caagtaattt tttcttagaa
      541 gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa ttttaaaaaa aggtactatt
      601 ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa gtgtatgctc attggaattt
      661 tttggaggga acaacatgga agagatcatc gtaagaggtg gcaatcaatt aaatggaaca
      721 gtaagaattg aaggagctaa aaatgctgtt ttgcctattt tagctgctag tttgttagca
      781 gaagaaggta ttacgacatt agacaacgtt ccgattcttt ctgatgtctt cactatgaat
      841 caagtgattc gtcacttaaa cgtagatgta gattttgatg aacaaaagaa tcaagtaaca
      901 atcgacgctt caagacaatt agaaatcgaa gcgccttatg agtatgttag tcaaatgcgt
      961 gcttcaatcg ttgtgatggg tccgctactt gccagaaatg gacatgcaaa agtagctatg
     1021 cctggtggat gtgcgattgg taaacgacca atcgatttgc atttaaaagg atttcaagct
     1081 ttaggagcaa aaattattca aaaaaacggc tatatcgaag caattgcgga tgaattgatc
     1141 ggtaacacga tttatttaga tttccctagt gtgggtgcta ctcaaaatat catgatggct
     1201 gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag ctcgcgaacc agaaatcgtt
     1261 gatttagcaa acattttgaa taaaatgggc gctcaagtct atggagctgg tactgaaaca
     1321 atgcgtattg aaggcgtgga tcatttacat gcagtcaacc actcaatcgt acaagatcgt
     1381 atcgaagcag gtacctttat ggttgctgct gcaatgacac aaggcaatgt gctgattgca
     1441 gatgctatct ctgaacataa ctgcccattg atttctaaat tgatcgaaat gggtgcagaa
     1501 atcattgaag aagaaggcgg agtccgtgtc atcggaccaa aacatatctt acctacagat
     1561 gtaaaaacga tgcctcatcc tggtttccct acagatatgc aagctcaaat gacagctatc
     1621 caattagttg cagaaggtac aagtgtggtg actgaaacag tgtttgaaaa tcgtttccaa
     1681 catttagaag agatgcgccg tatgaacgct catgtcaaaa tcgacggaaa tgtagcaatc
     1741 atggatggaa accatgaatt gcaaggtgca gaagtatatg caacagactt gcgggcagca
     1801 gcagcattag ttttagctgg cttgaaagca aatggaatca cacgtgtgcg taacttgaat
     1861 tatctagacc gtggatatta taatttccat atcaaactac agcaattagg tgcagacgtc
     1921 gaacgtgtag acatggatca aacttctgct gaaaaaacag cacaaacgat tgcttaagga
     1981 agtggctgaa tgagtaacca acgatatatc ttattaactt tgataaagat tttagtggtg
     2041 atcttattgc ttatccttct ttttgtggcc ggaacgatga tcggatatgg tgtcatcggt
     2101 ggcggcaatc catttaaagt attccagcca agtttgtgga ttcatattcg agactttttt
     2161 cattgataaa taagaatcac agtggttcta gtcgatatga agtgagccat gaagcagaac
     2221 atgataaaag aaaacagaag ggaacgttga cgttagtcgc gatccctttt tttagtaaaa
     2281 gaaaagaagg aatctaattg atgaagaaga tattgattta tttagttaga ggatatcagc
     2341 gctttatttc gcctgcattt ccacctagct gccgttatta cccaacctgt tccaattata
     2401 tgataaaagc aattcaaacc catggtgcgc taaaagggac ggtaatgggg actgcacgga
     2461 tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta tgtaccattg acattccgat
     2521 taacgcgaaa taagaaagat aaaaaagaac cagattataa aaaggttgtg aaaaatacgg
     2581 gtagcactga gcgataaaga aaaagaggaa atttattgtc aaagtaacaa ctccgcaact
     2641 acttatatgg gggacaaaaa atgatcgaag aactaaacaa gaaagattta ccatggtcct
     2701 tgttattaga tgcagatcct gataaagaaa aagttcaagt atatgtttcc agagggagtg
     2761 gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact gatctatgtg gcaagggaaa
     2821 aagaatacga aatcgtcaat gtagcagtaa ccccaacgta tcaaggaaag gggattggcg
     2881 gaaaattact tgagacagct tttcagaagc tttcacagct agcttcaaac caaactcgga
     2941 tcattatacg tactggcact actagtagcg ctgcgttaca cctctatcaa aaaatgggct
     3001 atgtggagat tggtcgagtc aaagactatt ttatccatca ttaatctgag cccatttttg
     3061 aaaatggaga tcaattaagg gatcaagtga ttttagcaat tcacttacct aaaaatagct
     3121 ttaactaata aactttcaat tcgagaaaat gctttcaaat gctaaataga tacattacaa
     3181 tagctttagc aaacaaaaag gagaatattt ttatgggaat attagttttt gattttggtg
     3241 gatcggctgt caaatatggt tgttgggatg gcaaagagat taaaggtaag ggacaatttg
     3301 caacgcctga atcttgggaa gaaatgaaaa cgcaattatt tcaagtttat aaaaaaatac
     3361 tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc tggagtagtg aataatgaga
     3421 aacaggtgat tgagggaatc agtgcgattc cttatattca tggatttaat attttccgag
     3481 atttggaaac tctgtttcaa ctcccagtaa cgattgaaaa tgacgccaac tgtgccggaa
     3541 tggctgaatt ttatgaaggt gctgcccgtg attatcaaga tgttgctttt gttgtggcag
     3601 gaactgggat tggcggggcg ttgttcacga aaggacaaat aaataaaggg gcacatcttt
     3661 acggtggaga atttgggttg atgttcttag atggcgataa gacattcagt aaacttggta
     3721 cagcggtaca gatggcttgg cgttattgtg aacgcaaagg tctcgataaa aatgcttata
     3781 ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc aatagcaaaa gaagaagttg
     3841 agagttttta tacgtatctt acaaagggat tattcagtat tcaattttcg tttgatccag
     3901 aagtgatcgt attaggcggc ggggtttctg caaaagaagg gctcattgat gagatcaaaa
     3961 gccgaatgaa gaagctgaca gaacaattcg atctgcatga tttcgaaccg cagattttat
     4021 tatgtgagta cagaaacgat gctaatcttg taggtgcagc agcaaactat ctcgctgtac
     4081 gtcaaggaga gaattaagag ctttagagaa ggtgcagctt gcataaaaga agagaatgaa
     4141 atgaatagga gatttttacc taaggaatgt tttatttgct gcagtatgat aaaatatctg
     4201 agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa gtgcgtatag aagaagtgaa
     4261 aaaagatatc aaaggtaaga attatgaagt cactcagtta tttataggga aaaaaatgat
     4321 tggtgagatc ttagcctatg ggccaaaaga atatgaaatt ttctttggtg aagaagattt
     4381 tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg attcgacact ggaatctgca
     4441 cgaataaaaa aatatcaaaa aaactttcaa taaagggttg caaaaatctg gaaaattagg
     4501 tattataact aagtcaggtt gatacaaacc ttt
//
LOCUS       DACCSK010000163         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCSK010000163 DACCSK010000000
VERSION     DACCSK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.167"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            145..363
                     /locus_tag="IVP39_002605"
     CDS             145..363
                     /locus_tag="IVP39_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587653.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(652..1164)
                     /locus_tag="IVP39_002606"
     CDS             complement(652..1164)
                     /locus_tag="IVP39_002606"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7587654.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1178..3475)
                     /gene="recJ"
                     /locus_tag="IVP39_002607"
     CDS             complement(1178..3475)
                     /gene="recJ"
                     /locus_tag="IVP39_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7587655.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3617..4051)
                     /locus_tag="IVP39_002608"
     CDS             complement(3617..4051)
                     /locus_tag="IVP39_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7587656.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4061..>4475)
                     /locus_tag="IVP39_002609"
     CDS             complement(4061..>4475)
                     /locus_tag="IVP39_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7587657.1"
                     /translation="AIDMAERRQGHIINIASMAGKMATAKSTVYSATKFAVLGFSNAL
                     RLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAKMIVRNMRHP
                     KREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
BASE COUNT         1240 a          984 c          787 g         1464 t
ORIGIN      
        1 tttgagacgt tggtctcaaa ctcttttttc gcccgggttc ttcttctttc aaatttttat
       61 cacttccctc tttacaaacc gaacaaatat tcgtatacta aatatacgaa caattgttcg
      121 cataaattaa agaggtgaga attcatgaaa tcaatgagac gtcttggctt agtattaact
      181 gttttgtttt taggtattat tattggtaat ttcggcatac gtccagcttt acttgtgttt
      241 gttccgttat ttactttgtg gttcatgttg tgggatgaaa agaagtaccg tcaatctgag
      301 cggcgcagaa ttcatgaaga tcatttctat catgagccct actacagatc caggcaatca
      361 taataaggat tgactgctaa ttattcaaaa gacaagaagt taaactttta cgggggtgtg
      421 acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt tctaaagcca gtttttcggt
      481 attaagtcaa attgcggaaa aagtgatttt ttaaacttct actggtcttt gcttgtttcc
      541 atttataaaa aaatagcgac cctcaaaagt cagatagaga atctgacttt tgagggtcgc
      601 tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg cttaatattc
      661 cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat ccatcaattc
      721 gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt cgatcgtcgc
      781 tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt gacctggttt
      841 aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat aagtcacttc
      901 gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga ctcctaactc
      961 ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta ccatatcaat
     1021 tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag cgtcaccatc
     1081 tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt cgggaatact
     1141 ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac catttcttta
     1201 tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg agccgtcgct
     1261 ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt tgtaaaacac
     1321 cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa attaataatt
     1381 tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata tcaattcgtt
     1441 cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct accccattca
     1501 aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa taagaccgaa
     1561 aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga gagtttggtt
     1621 ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc gcagcttcct
     1681 ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa aggctacctt
     1741 ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact tgcagaccac
     1801 ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat tcattgatcg
     1861 aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga gtaccaaaac
     1921 caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc agatgctgtt
     1981 ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa aactgaggca
     2041 aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc tctactgtta
     2101 cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc aagtcaattt
     2161 gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt tcacttggca
     2221 tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct cgcatctggt
     2281 caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta gctgatccat
     2341 cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt ttcccagcaa
     2401 caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata tgattctcct
     2461 tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt ctttcttcat
     2521 tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac gtcgcaagta
     2581 acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg ggagcaatcg
     2641 aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct tcaaatagcg
     2701 cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa acaaggattc
     2761 gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact aaatccaaaa
     2821 attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact ccgactcctg
     2881 ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc gcataagcgt
     2941 ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca ccagcttgct
     3001 gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg atcaactgga
     3061 ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca tgctcaaatc
     3121 gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct ttcattaccg
     3181 ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt tctccactga
     3241 ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga tacggatcat
     3301 gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca tggtaacctc
     3361 tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc atttggagaa
     3421 aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt ctcactttct
     3481 cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga aagaaagaat
     3541 aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt gtacgaccat
     3601 ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat tgagtttttt
     3661 gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac tccgcctgat
     3721 aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct tcttttgctt
     3781 cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc agctgtttga
     3841 tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc agtgcaccaa
     3901 tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg aagccaaaac
     3961 ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa actaaaacaa
     4021 atccaagaat cacattgctt tgctttttca attccttcac ctactttttg ttaaaaattc
     4081 cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat gcaacttcta
     4141 aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg atcatttttg
     4201 ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt ccgcttggat
     4261 cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta acagctacgc
     4321 caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca aatttcgtgg
     4381 cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg atattaatga
     4441 tatgaccttg tcttctttct gccatatcga tcgca
//
LOCUS       DACCSK010000164         4470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCSK010000164 DACCSK010000000
VERSION     DACCSK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.168"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(279..809)
                     /locus_tag="IVP39_002610"
     CDS             complement(279..809)
                     /locus_tag="IVP39_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7587658.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            914..1465
                     /locus_tag="IVP39_002611"
     CDS             914..1465
                     /locus_tag="IVP39_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7587659.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1618..2091)
                     /locus_tag="IVP39_002612"
     CDS             complement(1618..2091)
                     /locus_tag="IVP39_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7587660.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2229..3701
                     /locus_tag="IVP39_002613"
     CDS             2229..3701
                     /locus_tag="IVP39_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7587661.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3698..4408
                     /locus_tag="IVP39_002614"
     CDS             3698..4408
                     /locus_tag="IVP39_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7587662.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1406 a          814 c          863 g         1387 t
ORIGIN      
        1 caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat
       61 aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa
      121 agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta
      181 ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct ctaatactca
      241 ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg tttataattt
      301 tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg ttcttcgcta
      361 ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc aaattcagga
      421 acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc tgcgatatgt
      481 tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg gaaaaagatc
      541 attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt ccctgttggc
      601 tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct
      661 tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt atggtcattt
      721 ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg agttccaata
      781 aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa ttaaaacatg
      841 taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa gatttgtcaa
      901 ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag ggttattaag
      961 taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt
     1021 ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag agcaaggaat
     1081 cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt atgaattaac
     1141 agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg aactaccggt
     1201 taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt
     1261 agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc atctattaga
     1321 gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg gacactacct
     1381 gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa cagatgtatt
     1441 agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga attcctttct
     1501 atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg ccttttttca
     1561 agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta gtggcgttta
     1621 gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga gcttgtaagt
     1681 taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt ctttgaagtt
     1741 accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga ttgcttttga
     1801 aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt ctgcagcttt
     1861 tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta tgactgtagg
     1921 taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga atcccttttg
     1981 ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt cactgatgcc
     2041 aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca tttttgtcac
     2101 tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt caagtaggtt
     2161 gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa
     2221 ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat aatcggacac
     2281 ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag accttattga
     2341 cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat gtgcaatggt
     2401 taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca tggctgatca
     2461 ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg atcggaacga
     2521 ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg atccaagctt
     2581 gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt ttcccgccag
     2641 aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct cctgctttag
     2701 gccctacatt atctggttat atcattgata actaccattg gcgtgacttg tttggtatgg
     2761 tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa agtgttattc
     2821 cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt ggattcggta
     2881 gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg aaagtaattg
     2941 gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa ttacatttgg
     3001 aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt gcagctgttt
     3061 tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca ttgtatattc
     3121 aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg ggtgcattga
     3181 tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc gcgaaacgtt
     3241 tagccatcac agggatgttc attttgacag cggcaactgc accatttgct ttcttgacaa
     3301 aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt gggatctcca
     3361 tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac ttgatcagtc
     3421 atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt acagctattt
     3481 tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct gtattggata
     3541 atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt taccatgccg
     3601 cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt ttattgaaga
     3661 aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag ttttgattgt
     3721 cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc aaaccttttt
     3781 atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc
     3841 caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc
     3901 tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa aagaaaaagt
     3961 gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta cggataacga
     4021 aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg
     4081 ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca atcacgaata
     4141 tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa gtacggacca
     4201 agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa
     4261 agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc
     4321 agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag cattcgcaaa
     4381 attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgtaggag gttgtgaaaa
     4441 agctgctctg catcaagcag taagaacaat
//
LOCUS       DACCSK010000165         4460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCSK010000165 DACCSK010000000
VERSION     DACCSK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.169"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..420
                     /locus_tag="IVP39_002615"
     CDS             <1..420
                     /locus_tag="IVP39_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587663.1"
                     /translation="RFEGVTGRKISLKDESNTTVSDANVLANIILDFIFEHHIPFKEG
                     YEILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCR
                     IHHTEKHKIGVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            423..1172
                     /locus_tag="IVP39_002616"
     CDS             423..1172
                     /locus_tag="IVP39_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587664.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPIRHKYGEYKNVLLSDEQME
                     KLKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1184..1453
                     /locus_tag="IVP39_002617"
     CDS             1184..1453
                     /locus_tag="IVP39_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587665.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1616..1921
                     /locus_tag="IVP39_002618"
     CDS             1616..1921
                     /locus_tag="IVP39_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587666.1"
                     /translation="MDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKTKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSSWIDSKGRQFRYVEEKKC"
     gene            1915..2205
                     /locus_tag="IVP39_002619"
     CDS             1915..2205
                     /locus_tag="IVP39_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587667.1"
                     /translation="MLDMRIEDYRITSDSRNIVLSKVRRDEEGNIRYTEAKEESRADI
                     GYFQTVSSCLKAIQRDYVLSEERTIKSIIEYKKALENITRQFEQACEIEEEK"
     gene            2205..2522
                     /locus_tag="IVP39_002620"
     CDS             2205..2522
                     /locus_tag="IVP39_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587668.1"
                     /translation="MEFVDLNVRGIKCDNPECDYSDMAVKYENYPQWLNKPCPKCGAN
                     LLTQEDLDATEQLMEIVNLTNEILKDSGLEKQDMNKYIVPVEANGTGELSFGEIKKLE
                     EEK"
     gene            2519..3334
                     /locus_tag="IVP39_002621"
     CDS             2519..3334
                     /locus_tag="IVP39_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP7587669.1"
                     /translation="MNENKLIKLGVAGVVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKEHGWVTVNGADTVVTDETGK"
     gene            3473..3787
                     /locus_tag="IVP39_002622"
     CDS             3473..3787
                     /locus_tag="IVP39_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7587670.1"
                     /translation="MDLITQYSEIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            3964..4275
                     /locus_tag="IVP39_002623"
     CDS             3964..4275
                     /locus_tag="IVP39_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587671.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEEA
                     E"
     gene            4272..>4460
                     /locus_tag="IVP39_002624"
     CDS             4272..>4460
                     /locus_tag="IVP39_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7587672.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKW"
BASE COUNT         1730 a          661 c          983 g         1086 t
ORIGIN      
        1 cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag acgaatcgaa tacaactgtg
       61 agtgatgcca atgtcttagc aaatatcatc ttagatttca tctttgaaca tcatattcct
      121 ttcaaagaag gctatgagat tttacctgcg aatcaagaat attacttcta caaatgcatc
      181 acaaaaagag tctgctgcat ctgtggcaaa acaggagctg acatcgatca ctttgacaaa
      241 gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag agtacacatt tgcagcactc
      301 tgcagaatcc atcacacaga gaagcacaaa ataggtgtga ttaatttcaa aaataaatat
      361 caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa agttaaatat tggagggtaa
      421 aaatgacaga acatcgaagt tattatgcga ttataccagc caacgtaagg tacgacaaaa
      481 gacttaaacc aaatactaag ttgttatacg gagagataac ggccttgtgt aatgaaagag
      541 gcttttgttg ggcaggcaat gagtactttg cagatttata tggtgtgaat aaagagacca
      601 tatcacgatg ggtaagtgat ttgattaagt ttggatactt gaatcgggaa atcatttaca
      661 aagagggtac caatcaaata atcaataggt acctacgaat taatcaatac cctattgacg
      721 aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa taatacatct attaataata
      781 catttaataa tacaaaagaa tatataagag agttaccgcc ttcgaaaaaa tcgaaggcta
      841 agcccatccg tcacaaatac ggagagtata aaaatgttct tttgtcagat gagcagatgg
      901 agaaactcaa aatagaattc cctaatgact atcaagaacg aatagaacgg ctatctgagt
      961 attgtgaatc atctggtaag acttataaaa actatttggc aactattcga agttgggcaa
     1021 ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata caagcgcaca ggaagacgag
     1081 agaagcttcc tgaatgggca atcgaccaag aagcctatct taagaaaaaa gcgctagaac
     1141 gagctaatag acaatcaaaa gcaccattct aagaggtgga aaattgaaga tcgattatct
     1201 agaactaatt aatgaaatag caaagtataa aactggtgag gaaatagaaa ttctgagaga
     1261 cgtatatgat caactcgaag aagctggaat tgaaggaatt aagaatgatc gttcgagttg
     1321 gagtaagctc agatactatt tcgcacttta tatcgatgga acacaattaa gaaatttagc
     1381 atataccaaa ttactatttg ttgattgtgt taaaggcttg caaaaacatc ttaatgaact
     1441 tgagcaggtg taaacaagat ggacctaaag acatttacag cacagatcga attaatgcat
     1501 caagaagctt taagacaaag tgtatcgtac gaagacaagt ggctcaacac gtttcatggc
     1561 ggacgtgaga gcgcacttga tcaagtactc aaattattga aaggagaatg tggggatgga
     1621 taaaaaagcg gcaatgaaac gaattgctga attaaccaag tcagaatctt ggcaagaaga
     1681 caaagaaata gttgcagaag tccaaaaact cggcaaatca atgtggactg aaaaaaccaa
     1741 acggaaaacg ccgagaaaga ttgcaatctg gcatggtgat cgaattctag taacaggtac
     1801 tgctgaacag ttatctgaaa ttactggatt aagcaaaaac attatttggg atagagctag
     1861 gagctcatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga aaaaatgcta
     1921 gacatgagaa tcgaagatta tcgaattacc agtgattcta gaaacattgt cctatcaaag
     1981 gtaagacgag atgaggaagg aaatatccgc tacacagaag caaaagaaga atcacgagca
     2041 gatatcggat acttccaaac tgtctcatcg tgtttgaagg cgatacaacg cgattacgtg
     2101 ttaagtgaag aaagaacgat aaaaagtatt atcgagtaca aaaaagcgtt agaaaacatc
     2161 actagacagt ttgaacaggc atgtgagatt gaggaggaga aataatggaa ttcgtagact
     2221 taaatgtacg tgggatcaaa tgcgataatc cagaatgtga ttattctgat atggcagtaa
     2281 aatacgaaaa ttatccgcaa tggttgaaca agccatgtcc gaaatgcggc gctaatcttc
     2341 tgacgcaaga agacttggat gcaacagagc agcttatgga gattgtcaat cttacaaacg
     2401 agatcctcaa agattcagga ttggagaagc aggatatgaa taaatatatc gtacctgtag
     2461 aagcgaatgg aaccggtgaa ttgtcatttg gagaaataaa aaaactggag gaagaaaaat
     2521 gaacgaaaat aaattaatca aactaggtgt ggcaggagta gtaatagtag gtattggagt
     2581 tatcggagga tttaagtttt tcgaaaaaat tgataacgga tatgtgggtg tgcgctattc
     2641 aatgaatggc ggtatcaaag atgaagcact gacgcaaggt gtgaaatttg ttgggattga
     2701 caaagtgatc caatatccaa ttcgcttgca aactatccaa tcaaaaaaca tttcagtatc
     2761 aacaagcgat ggcaaaaaga caacgattga tatcaaatat gactacaaag ttgattcaac
     2821 taaagcagca aaaatgtaca aagaatttgg gaatataact tcggaagata tcgaaagtgg
     2881 atggttaaaa tctaagcttc aaaaagtagc gcgtgaagtt tatgccaaat atagcctgct
     2941 tgatgtcctt tcaggagatt cctctaaagt tgaagctgag gtactaacga actttgctaa
     3001 atcagttgaa tctaaagggt ttgaagtcga agacgtaaca cttggtgttc cagatgtcga
     3061 taaagaaaca caaaaatcaa ttgatgcgat cattcgagct ggtcaagaaa atgaaaaagc
     3121 gaagctagat gcagaaactg caaaaactca agctgatagt gaagcttaca agaaaacaaa
     3181 agctgcagaa gcggaagctg aatcaaaccg taaagttgcc gaatctgtaa cagataattt
     3241 aattcgttat gaagaagctc aagctcgaaa agagcatgga tgggtaacag taaacggagc
     3301 agatactgta gttacggatg aaacaggtaa ataatatggg attctttata gctaaaattc
     3361 tcttgttctt aggtttggta ggagtggcat atctcgtgta tgccctcctt tccaaaactg
     3421 atgacaaaga agatgacaac gacgatgaaa tgaaatttta ggaggagaaa taatggatct
     3481 cattacacaa tacagtgaaa tcatcctcaa gaaaatcatg atgaagattc agaaagataa
     3541 aaaatcaaaa gaacgagcgg aattagttaa gttggaaatg gctgaaacag gagcaggagt
     3601 gcgaagtagc aggcattgga aagcagcagc aaacattgaa ttctattaca acgaaattca
     3661 aaaagggttc gatcagatgc gtgagctgga tcggcaaaca aattggagca agaaacttca
     3721 tcaagatcgt ttcaaatttg tagaaaagta tagagagata ctgaatgagt atttcgagga
     3781 ggactagatg tttaatttaa tgttagcgtc aattcacttt ttattctata taacgatatt
     3841 tgtagctata ttaattaata cgataaaaag cggtttttta acaccaatcg gcacaatttt
     3901 aacattagct tcatttgtta caagttgtat tatccaactt aaatatataa aggagaatga
     3961 ataatggacg aactaatcac aaaagtagag cagtgggcta aagataaggg attggatcaa
     4021 gctgattcca gcaagcaaat gttaaaaacg atcgaagaga ttggggaagt tgccgcttct
     4081 ctagctagaa aagatgaaca tggtttaaga gacggaattg gagacgtagt agtaaccttg
     4141 attattttag ctatgcaaaa tgatatggat ttgtacgagt gtctgaacca agcatacaac
     4201 gaaatcaaag gacgcacagg gaaaatggta gatggtgtat tcgtgaagtc gagtgatttg
     4261 gaggaagcgg aatgaacaac agacatcgca gaataacaaa actaagaaaa caggaactga
     4321 atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa acatataaag
     4381 tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga atttcgatat
     4441 taagtgatga tcgtaaatgg
//
LOCUS       DACCSK010000166         4399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCSK010000166 DACCSK010000000
VERSION     DACCSK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.170"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..385)
                     /locus_tag="IVP39_002625"
     CDS             complement(<1..385)
                     /locus_tag="IVP39_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP7587673.1"
                     /translation="MQELVILKNKEAVTTSLQVADSFEKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLE"
     gene            439..900
                     /locus_tag="IVP39_002626"
     CDS             439..900
                     /locus_tag="IVP39_002626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587674.1"
                     /translation="MKKLLYILLFSTFAIVALLLLFEHLKRVNLDGTLLEEQIAILNT
                     PKLLIVFFFMCMLSALIFMFYISTFWVYKEIKNKEFTDKDMIFMGFLVKILFIFSIVI
                     TLLFCLNETQFSVATTLISFIAIFSFIFSDNQKKSINGFLQKIKKKINGEP"
     gene            complement(1122..1421)
                     /locus_tag="IVP39_002627"
     CDS             complement(1122..1421)
                     /locus_tag="IVP39_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587675.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKGLSPSDVLKEAS"
     gene            complement(1526..1729)
                     /locus_tag="IVP39_002628"
     CDS             complement(1526..1729)
                     /locus_tag="IVP39_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587676.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1744..2490)
                     /locus_tag="IVP39_002629"
     CDS             complement(1744..2490)
                     /locus_tag="IVP39_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7587677.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2568..2876
                     /locus_tag="IVP39_002630"
     CDS             2568..2876
                     /locus_tag="IVP39_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587678.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2847..3104)
                     /locus_tag="IVP39_002631"
     CDS             complement(2847..3104)
                     /locus_tag="IVP39_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP7587679.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            3836..4156
                     /locus_tag="IVP39_002632"
     CDS             3836..4156
                     /locus_tag="IVP39_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587680.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            4174..>4399
                     /locus_tag="IVP39_002633"
     CDS             4174..>4399
                     /locus_tag="IVP39_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7587681.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFH"
BASE COUNT         1284 a          786 c          722 g         1607 t
ORIGIN      
        1 cttctaattg atagatcatc atgtcttcaa ttgtttgagg aacagtattc ttccgaataa
       61 catcttccat ttcgttgaaa gcttcaataa attttagttt gaaatttatt gctttacttc
      121 cagtgaatcc catagctagc aaggaaaatc cgtctctatt catgaaataa actctccgac
      181 ttcttccgta tgagtctggc tcgttccctt ccacaaacat ctgtccaaaa ttggacccat
      241 cttcaacatt ggccgaattt tcgaccgatc tttttattga ttcgattgct tctagcacat
      301 gcttgtgttt cttttcaaaa ctgtctgcga cttgtaagct cgtagttaca gcttctttat
      361 ttttcaaaat tactaattct tgcatttttt cttctctcct tttgatataa tttaggtaaa
      421 aaatggtggt gaaaatattt gaaaaaattg ctatatattt tacttttttc aacctttgca
      481 attgttgcat tattgttgct tttcgaacat cttaaaagag taaacttgga tggaactttg
      541 ttagaagagc aaattgcgat tctaaatact cccaaattac taattgtatt cttttttatg
      601 tgtatgttat ccgctctgat atttatgttc tatatatcaa ctttttgggt atacaaggag
      661 attaaaaata aagagtttac tgataaagat atgattttca tgggtttttt agtcaaaatt
      721 ctttttatat tttccatagt aatcacttta ttattttgtt taaatgaaac acaattttct
      781 gtagcgacca ctttgatttc atttattgct attttttcct ttatattttc cgataatcag
      841 aaaaaaagta ttaacggatt tctgcaaaaa ataaagaaaa aaatcaatgg agaaccctaa
      901 tataaaaatc aggaaaaaaa tattgattac tcctcttagg aagaccatta gtcagtccct
      961 cccggctggc tttttcgctc tgtactcagc ttcatcaagc cccataaaaa tccagaccat
     1021 atatacgatt gtgccgatta atgcttgtct gcttccccaa agacctaaag cgtagatgat
     1081 gaatggtgtg ctgaatacta gcgctctgtt gaatttgccc attagcttgc ctccttcaaa
     1141 acatctgaag gcgataaacc ttttacacga tatttgtttg cgtctttcca ctttaaaaac
     1201 caaataaaag tatggtaatg aataaatgtg gttgagtgac ctggacgaag tattccttct
     1261 gcaaattcag gaattgtttc catttccttg cagtattcaa ctaaagtagt ttttgacatg
     1321 ccgtggaact tctccaagat taaactttgt cggtaccagt cgtctggttt tactgccttc
     1381 tcagcagctt ctatcagctc tgaaagtgtt ggttttttca cttattatcc ctcctaaaaa
     1441 ttatcgttcc atactcgatg tttaacattt cttgaagctc tgattttgta agaatgattt
     1501 tatcttccaa cgactttacc tccctctaca attcgtacat agtgataatc gaatctataa
     1561 ttctgtttgc ttctgcagaa gtctttttac cgtttaaaat taaagataag tagcttttgc
     1621 taattccaaa tctttcagca agcatggtgt aagttaagaa ctttgaactt tcgacatatt
     1681 ctttgatttt ttctctatct cgttgagtga tttctgcaat gtcagtcata ctaaaactcc
     1741 tttctaacca atttcctcta aatccatttg agggtaatag ccctctgcga ttaataagtt
     1801 gtaaataaac actcgccctt tctgtgtcca tttggtattc attacaactt tagtgccgcc
     1861 atcggctttc ggtatctcac ttgtgtgcga ttttgtgtat ccttggttca tatgttttcg
     1921 gtaaagtatc cattgtccgc taactttatg ctgaacacct aactcgttta gcaatttgtt
     1981 tagagcaatt gccgacattc cgtaatcagc tgcaatttga gaagtagcta ccgtatctgt
     2041 cgatgaaaga atcgtatcta agtagctaat ctttggttcg tactcggcaa tttgttgttc
     2101 gagtaattgg ttcttttctt ctaaatccgc tgctaaacgc aatgcttgtg caaaactttg
     2161 aggtacgttt gaatagctac ctgtttttct aattgttgga aggacttcac tcgttaccca
     2221 acgtttaaat tttttggcag agggaagttt tgattttaag attaaactgt aaaggcctga
     2281 ctcgttaatc aaaacaactg ctgttccatt gacggtgaac gaatcgttct gcgtcttatc
     2341 ctccaagtcc acatgatctc gaatagcttt ttgcggattc gaatatccta gaacgtctgc
     2401 tacatctttt cctacaaaat atggttcatc atttactaaa atagttcgaa cttcgttttg
     2461 ctcgaaatta aaaatttgtg gtgtattcat atttctcatt cctttctttg gtataatttc
     2521 cttatcagtg tgacaggctg aaataattgt taaggaggtg aaaatttatg aaaccagaat
     2581 ttgtaattac tatgcttgat ggagaaaaaa tcaaaatatt tgccgatact ttggtcgttg
     2641 gatacgatga tgcgtcaaat atcgaagaaa aagatggaat gttctattct tctcaaattt
     2701 acgtgaatac cttgcaaggt gatattgggg tatctactct tgcaacatca agaccgcttg
     2761 taggtcttat cggctttttg tcaaaagcaa cattcttttc tctcggcgat gatatagatt
     2821 cggatgaaac tgtcatctat aaaacatcag ctgttaaaag tatcggtttc aaatagtaat
     2881 tcttgctcgt tgatagcgga agccaacttt tcttccaaca catcggcgag ctttcttttt
     2941 gttggattca tttcaaagtt gatagtcttg agaagcattt ttgcatcctt aactttttgt
     3001 ccttgcaatt ccttgatgat tttatcggct tggctatcaa ttccatgtgt tagatcatct
     3061 atcatttttt gtaccttgat tgtttggctc atttccttgt tcattgttta gcctccttta
     3121 ataaatgatt ttttgtattt tttaatttcc ataaattcct caaattgctc tttggtatta
     3181 ttaccacgac catacatatt gtgaaattct ttatggcaac tgacacaaag tgtaacgccg
     3241 tttgaaatcg ctgcggattt agttgttgca tggctaaatc acgaatcaca agtatctact
     3301 aaaggcgaat caatttcgcc aaaagaaatt gttcaagctt atttagatat tcattatgct
     3361 gttatctatg gtcagcttcc agaagaccgt aaaaacgatg actaaacgtg agctaaaatc
     3421 tctgctatgg ctgcaaccat ggcagagtct tctttatcta ccgcgtgttc catcgccact
     3481 tttgcttgtt tcagtatgtc taatttcaaa tcttcgattt ttgccgagac tgttttttcc
     3541 atattcttct ctcctttctt ttatattcgt aaacaaattt aacaactttt taaaaaactg
     3601 cgttgactaa attaacatac agtgttaaaa tcagaacata gttaaataag acatataaac
     3661 attgatttta aaagctttct tggcggttgg catttattaa tcaatagtgt tttttgttgt
     3721 ctttttagtt gttaaacttg tttacaagaa tgatattaac accttttgtt aaaatagtca
     3781 acgaatttta acaaataaag ttaaatattt tttcttgaac gaaaggaata ttgatatgat
     3841 aacgtttgag agaataaaag agttagcgaa aaaacaggga aaatcattaa ataaagttga
     3901 agaagaccta ggctatggta aaaacgtctt atatagactt aagaactcaa acccctctac
     3961 agaacgtttg caagaaattg ctgactattt cgatgtctct gtagactatc ttctggggcg
     4021 cgaagaaaaa gaaaccccta aacatgtgga tttatcagaa aacgatactg ttttttcttt
     4081 tgatggaaaa gaaatatcta aggagacaat gcgtaaagcg attgcaattg ctaaagcttt
     4141 agaggaaaat gaatagttgg agtgatgggt tgtatgtatt tgaaattgaa agaaatgttg
     4201 agcgaataca atttgaaagt agtttacatg gaaatgaaag agcctggctt ctattatccg
     4261 aagcctagaa tcattttttt gaatgaaaat ctatatggcg aaactgcaga agcctttcat
     4321 ttatctcatg aacttgcaca tttcagcgcc tctcattttg aattttcagt tttgtacgat
     4381 acttctacaa cttttcact
//
LOCUS       DACCSK010000167         4319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCSK010000167 DACCSK010000000
VERSION     DACCSK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.171"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(293..838)
                     /locus_tag="IVP39_002634"
     CDS             complement(293..838)
                     /locus_tag="IVP39_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587682.1"
                     /translation="MIYAGVVCFGIAGNYIMNMFHLNKYVNFTIRNIKLKQHLKPIMV
                     LFVSVIAIELYSLIDVTMLTHMTSSARVGYLSNAVKIVKMIANTFTAMGQFSYQDLVF
                     ITLRRIPLNWKKLYIIFFEIMIVIVAEHIVLNLLGESFISAVRTLQVLAPLVVMMPLS
                     GGVFGQLLLTTDSEKIFYDVL"
     gene            complement(1298..1687)
                     /locus_tag="IVP39_002635"
     CDS             complement(1298..1687)
                     /locus_tag="IVP39_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587683.1"
                     /translation="MYDTIITIFSTERIWGYGANNHHIVLYSLVKAPNAQNGILNCLI
                     QFGIAGTLLLLITLHMMLRKGKNINHSVSIMALIMMMSVISSVEITIDIYFILFCAII
                     WAYQFKAKHYKNKEVKDEKPPCKICCF"
     gene            complement(1773..2151)
                     /locus_tag="IVP39_002636"
                     /pseudo
     CDS             complement(1773..2151)
                     /locus_tag="IVP39_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038005.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(2308..2604)
                     /locus_tag="IVP39_002637"
     CDS             complement(2308..2604)
                     /locus_tag="IVP39_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587684.1"
                     /translation="MDWLEQSDEGWVFGSRISEGMAAEIAKANELGKVVSNLYQPDSD
                     AHECGGCDCCHEKFDIDQKMAEMHDHLLHSICERTGLSEEEVDQKLMEISEVML"
     gene            complement(2672..3277)
                     /locus_tag="IVP39_002638"
     CDS             complement(2672..3277)
                     /locus_tag="IVP39_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7587685.1"
                     /translation="MIKVISSIVVNVLTALYQPFGFSVSLSILAMYFYLFATAKGVAG
                     QGFKKSLEVWILCFRSSAKFRRLFFLVFYTTMILFRTLLNRNLWVNPLSDVMGGWWIY
                     ETNPSTGEVNLTTECIENLILFIPFSALLMWFMEQKEKIKCILWTSTKVVFLFSVSIE
                     FFQLFLRLGTFQLSDLFYNTLGGFLGGLIYWIFYKVNKRFS"
     gene            complement(3398..4064)
                     /locus_tag="IVP39_002639"
                     /pseudo
     CDS             complement(3398..4064)
                     /locus_tag="IVP39_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603388.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
     gene            complement(4076..4309)
                     /locus_tag="IVP39_002640"
     CDS             complement(4076..4309)
                     /locus_tag="IVP39_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7587686.1"
                     /translation="MLVNVRVKDKTEAQNDPDMQNAVKAVADKLGNSGRILVRESGTE
                     LVVRVMVEAATEEICKQYVDSVVSVIKKNGYAV"
BASE COUNT         1412 a          965 c          683 g         1259 t
ORIGIN      
        1 ctttctgatt gggtttggtc actttaatct tacagggatg agtgcttttt gtgtacctta
       61 aaatcaaaaa aaatggggca ataatcagat ttttttctga ctattgtccc actctctttt
      121 tattactctt catcttctac tattccacta attaattagt tgacagatac aatgctttca
      181 catcctgcac gaacaaactc gagctctgaa ccaatcactt gcgagttgat aaattcaatg
      241 accatcagaa taattaaaat agcattaaaa ataacattac aaatacttcc tgttacaaca
      301 catcgtaaaa aattttttca ctatcagttg ttaaaagaag ctgaccaaat acaccgcctg
      361 aaagaggcat cataacaacc aatggagcca acacttgtaa agttcgtaca gcggatataa
      421 aactttctcc caataaattt aagacaatat gctccgcaac tatcacaatc attatttcaa
      481 aaaaaattat gtatagtttc ttccagttta aaggaattct tcttagcgta ataaagacta
      541 agtcttggta agagaactgc cccattgctg taaaagtatt agctatcatc tttacaattt
      601 tcacagcatt cgaaagatat cctacacgag cagatgatgt catatgagta agcattgtta
      661 catctattaa actgtacaat tcgatagcta taactgatac aaaaagaacc attattggtt
      721 taagatgttg ttttaattta atatttctta ttgtaaaatt cacatattta ttcagatgga
      781 acatattcat aatatagttt cctgctatac caaaacacac tactcctgca taaatcacaa
      841 tatccgtctt tttttaacaa aaagaagcat aaaaatcaac gatacaagct tgataataaa
      901 acttctaact gttatatatt tatattcttc gaatccttgg taaacccatt caatattaaa
      961 ataatttaga acaattaaaa aaaatcaaag aaagttgatt atatcccgaa ataaatacaa
     1021 cagacaaata tatgagtaaa cttatagtag ttgcaaatgc attaattata aataatcaga
     1081 gaaaattata tctgtaaata ttccatcgct ctgggctctt tttttgaaat aactttcaca
     1141 ctatacgttg gaatacccaa ggaagcagcc attgtaaaaa taggtcacag aattctgtac
     1201 tgatgtaact taaccaattc cttcagcttc taaaatatgt gaaacatacg atactgttat
     1261 aagaggaaaa acgactgtca aagctttata tagcatgtta aaaacagcat attttgcaag
     1321 ggggtttttc atctttgacc tccttattct tgtagtgttt cgctttaaat tgatatgccc
     1381 aaataatagc acaaaataga ataaaataaa tgtcaatagt aatttcaacc gacgaaatta
     1441 ccgacatcat catgattaat gccattattg aaacagaatg atttatattt tttcctttac
     1501 gtagcatcat atgtaatgta atcagcaata acaatgtgcc agcaattcca aattgaatta
     1561 aacagtttaa aataccattc tgtgcattag gagcttttac tagcgaatat agaacaatat
     1621 gatgattatt tgctccataa ccccaaatac gttctgtaga aaatatcgta attatcgtat
     1681 catacatttg caaacgacca tgcagatcaa gagtttcacc taaaatcttc gtgataatat
     1741 actaaaccgc aggttgagat aatattacag catcaaagaa caacaaaact gtatctgcta
     1801 gcgtattcca tactctggac catgtgcgga tgcacacgga agattcggca aatctcctcc
     1861 acatcaaatt ggcaagtaga aagaaactga ctgtcttcgg gtggaagaga gatcgtcttg
     1921 taactcattc cctcttcaag gactgcaccc gatgggcatt ggcagcaccg cctaccagtt
     1981 atctctcact cttgccgggt ctttcagaac accccgatgc tctaacacat cactcggcta
     2041 ggctccgttt ttgaagaagg aagccccata tcgttccacg acatcttcgg ccagcttaaa
     2101 gcttaagaag ctcctccatc tgttgggaga caccttcaca tatatggtca aagcccataa
     2161 agacctccgc cagactgtcc atgaccttct tcagtgctgc gatcatctgc tctctcttct
     2221 ccatggctta cacctactcg acagaatcag cgacatcaga ggcaggagca tccttcatgt
     2281 gctatgccag aaactcatcc agctggttca tagcatgacc tcgctgattt ccatcagctt
     2341 ctggtccacc tcttcctcag acaggccggt gcgctcacag atggaatgga gcagatgatc
     2401 gtgcatctct gccatcttct ggtctatgtc gaatttttcg tggcagcaat cacagccgcc
     2461 gcactcatgt gcatcggaat caggctggta aaggttactg acaaccttac ccagttcgtt
     2521 ggctttggca atctctgccg ccatgccttc ggaaatacgg cttccaaaca cccacccctc
     2581 atcagactgt tccaaccagt ccatagcgaa agtaggtcct cttccagctc atcgtggagg
     2641 aactgcgtaa aatacaggtg cattcctctt ttcacgaaaa ccttttgttg actttataga
     2701 aaatccagta gatcaatccg ccaaggaaac caccgagggt attgtagaac aagtctgaaa
     2761 gctggaaagt tcctaaccgt aggaagagct gaaaaaattc aatggaaact gaaaacagga
     2821 acacaacctt ggtacttgtc cagaggatac acttaatctt ctctttctgc tccataaacc
     2881 acataagtag tgcggaaaat gggatgaaaa gaattagatt ctcgatgcat tcagttgtta
     2941 gattaacttc accggtagaa ggatttgttt cgtagatcca ccaaccaccc ataacatctg
     3001 acaatggatt cacccacaag ttacgattca ataatgtacg aaataaaatc atggttgtat
     3061 aaaacaccaa gaaaaataat ctacgaaatt ttgcggatga gcgaaagcat agtatccata
     3121 cttctaatga ctttttaaat ccttgacctg ccaccccttt agcagtcgca aataaataaa
     3181 aatacattgc taatatcgat aacgacactg aaaaaccgaa cggttgatac aatgccgtca
     3241 atacatttac aacaatggaa gaaatcactt taatcatcat tcttcctcct aaactattat
     3301 taaacctcgg ctactggatt ttattcaaca ctcgtcactc acttcgtaaa agctattgat
     3361 ctaggatggc tgctgctcac acaggagctg caacgattta ttctttagta aatacgccac
     3421 gttcgccttt aactatactg ttctctggta caacaccacg aacactgctc agaggataaa
     3481 cctgtgtatt tcttccaatc acagtaccgg gattcagaac actattgcaa ccaatctcag
     3541 taaaatctcc aaggatagct ccaaattttt tgcgaccagt ttcaaaagaa cattcgcctt
     3601 ttacaactac tggggtttta tcgcctttga cattgctggt tatagaacaa acctgcaccc
     3661 atatgagttt tgtaacccag aatactatct cctacataat tataatgggg cacctgaatg
     3721 ccatcgaaca aaatacaatt ttcaagttcg gatgagttgc ctacgactgc accatcacca
     3781 accaatacat tgcctctaat aaaggccccc ggtcttactt ctgtattttc acctataaca
     3841 cacggatccg taatcgttgc caaaggagaa agcttggcac tcttagcaat ccaaacatcc
     3901 tcggcaatct tgttatacct gtcttattca agagatttac taatctgcaa aacggcattt
     3961 ccgatgtcag caagagcttc ccacggatat tcacaggtgt ccaaaaaacg gattgcctta
     4021 atatgggtta aatcaaataa atcatatatc tttattgtac ccatggcaat acccctcaga
     4081 ctgcatatcc attcttcttg atgactgaaa caacagaatc gacatattgt ttgcagattt
     4141 cctctgttgc agcttcaacc ataacacgta ctacaagttc agtaccagat tcacgtacca
     4201 gaatacgacc agaatttccc aatttatcgg ctactgcctt caccgcattc tgcatatcag
     4261 gatcattctg tgcttcggtt ttgtccttta ctctgacatt aacgagcacc tgcggaaaa
//
LOCUS       DACCSK010000168         4295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCSK010000168 DACCSK010000000
VERSION     DACCSK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.172"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            75..488
                     /locus_tag="IVP39_002641"
     CDS             75..488
                     /locus_tag="IVP39_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587687.1"
                     /translation="MLIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSD
                     EELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDV
                     NTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            502..1191
                     /locus_tag="IVP39_002642"
     CDS             502..1191
                     /locus_tag="IVP39_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587688.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1316..3667
                     /locus_tag="IVP39_002643"
     CDS             1316..3667
                     /locus_tag="IVP39_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7587689.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3747..4142
                     /locus_tag="IVP39_002644"
     CDS             3747..4142
                     /locus_tag="IVP39_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587690.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1503 a          549 c          864 g         1379 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc tacaatatta attgggggct attttctttt gaatttagat tctgttaatt
      121 caaacgctca aataaaagat caaagagtag ctgaacataa taataaaaaa atgagtttga
      181 ccaaaaatat ctattctatg tcagatgaag aattgtataa agaatttggt gatttatccg
      241 gggaacagtt agaagaaagt attccaggaa ttagtgactc agataaaatt gttgtcttgt
      301 ctgctggtgg gcaattaatg ggagatggca aatccgaatt agttagttat aaacaaaatc
      361 acagacagtt tattgatgtt aatactgatt ccaacgctat ttcgattaag gatctaaaag
      421 agcttttaaa tagtaaaaga aatgaattaa agcagataaa agaaactaat tcagtggaat
      481 tagagtaaag gaagattcat tatgaaaaag atttttggtg tttctgttgg gctacttatg
      541 atgttaacat ttggaaatgc agtatatgct gctgagaaag aagttatttc agatccagag
      601 cagtacgcct cacgatactt tgctgatttt gaagaagaag ataaagtact tgcattgcca
      661 gaaggtggtt ttttaaaagg ggaagctaaa attgtatcta tggataatcc agatataatt
      721 caaggttcat ataattcaga aactgatcct gcttcagttt cagtgacaga agcaaaaaaa
      781 attgttgctg aaaaattcaa aaatgatatt gaaactactc caattatgcc tcgaggagca
      841 tttccacccg atgttggtac tagcggacac cttcttgtac tagcaggagg aggaagctat
      901 aaatcacagc catttagtgg tgcgggatgg cgttttggag gtatgggtgt aaaaccagct
      961 gatggtacag gcaacttttt acgttggaaa tcctacattg acgatggaag agtaggaaat
     1021 gtaaatcaag ccaacgcaac taaatctggt tctattcaag gaatggcatt gtatgctcgt
     1081 gaaggattca aatactttgc accaaacggg cctggttgga caatgaatat aggtatttat
     1141 tacacttata atcctgttgc aggtacgtat tatgttgttc aaaatattta ataaaacagc
     1201 acaaagagat ttctttgtgc tgttttatta tttttagttg ttgacaaaca ctaaaatata
     1261 aaatataatc aacacataaa cgaacgtatg ttcggcaaga aagcagggga tttttatgga
     1321 tgagatggtt ttaagtactc agaaatggct aaacaagaaa tacagtaatg ttacagggtt
     1381 tgataaggtt ccagaaaatg ggcgaacagg gtggcctaca atctatggac ttattgaagg
     1441 actacaagta gaacttggta ttactaatct agtagctaat tttggaccta ccactgaaaa
     1501 aatgtacgat aatcaggtta ctcctaaatg gggcaaaaat ctacctaaaa atattgtatt
     1561 tcttattcaa ggtgcctttt ggtgtaaggg gattaatcca ggtggcttcg atggggtata
     1621 tactccatat ttagatactg ctgtaaaaga gttgcagact gatgcagggt ttaatggaag
     1681 cgctgtaact ggagttttgg atgcgaaatg ggccaaagcg ttatttgata tgagtgcttt
     1741 tgttcttgtt cccggcggag atagtaaaat tagaaaaatg cagcaatggt taaatgtaaa
     1801 ttatttagaa tacacgggaa taatgccttg tgatggtatt tatcaaagaa attcaaatca
     1861 agcattaatt tttgcattac aagctgaact aggatattct cccagtgaag caacagggtc
     1921 ttatggtaat gggacaacta gtaaaactta tcctgtgtct gaaggtaact cgggaaacta
     1981 tgtaagaata attcaatatg gattatatgt taatggttat tttgaagacg gtacattcga
     2041 tggtattttc actaaatata tgggtttaga agtactggca ttccgaaaat ttatggtatt
     2101 gcctgaatat actgaaatag cagatgttgt tgtaataaaa ggactattgt ctagcgctgg
     2161 agatattaga agatctgcag atggcgcaga tacttctacg caattaacta gatctcaaat
     2221 ccaaacattg gttgataatg atataaaaat agttggtaga tatcttactg gtactgttgg
     2281 aattggaaaa gatgaacgaa ataagtatct aactacagag gaattgaata atattttttc
     2341 taaaaattta tctgtgtttc ctatctatca agatggaggt gcagcattag cttattttac
     2401 ttatgatcgt ggcttatcag atggtaagaa ggctatagat gcagcaaaaa atctaaatat
     2461 accattaaca acagttattt attttgctgt tgatctagac atgttagggg aagaaattct
     2521 tgcatatgct ggagaatatt ttaagggggt atctgctgta atgtcatata gcgggtatca
     2581 aacgggtgtt tacggaacaa gaaatgtttg ttctcaaata atcaataacg ggtatgcttc
     2641 gtttgcattt gtttcagata tgtcgactgg ttatagtggg aatttaggtt ttcctatgcc
     2701 tagacaatgg tcttttgatc aatttgttga atttaccata ggaagtggca acggagctgt
     2761 tggtatcgat ttaatcgcta catcgggtag agatagcgga tttaatgagt tatcaaatga
     2821 tactaataat gataattaca ttgccaaata caatcagaag gttataaatc agatggtacg
     2881 tgcgtatcaa tatctatcac aagctggtct tgataatcct tggaaccctc atttgacatt
     2941 ttatagatat gtaaattatt ctggtttgtc atgggacatc atttcttctc ctgtaacaga
     3001 gcatgataga aaaatttatg atgaatatag gaataaatta actaatagcg agggcctgta
     3061 taattatttt atagatccta atactggttc aataataggt ttgcctcact taattgtgac
     3121 gttacaatca cagatgtttc tagccgacac aatcaatgat agtgtttctg actttgcagg
     3181 ttggttagga gatttaatga cttgctgggg tgaagttaag aaattaggaa tacaaatgga
     3241 gcaaggtgta tttgcattag taggtacggc tgcaggtgga acatttagtc tagaagattt
     3301 ataccaagat gttgacgcgg taaatgttta taccttagca aaaagaaatt ctgattcaac
     3361 agctttatcg gctttaaaaa cgtactattg tgataagggt gctattgata gatttaatca
     3421 atttgttaag aacagatttg gttcttcaga taaaatcgct tcagtaacaa atagtttatt
     3481 agaaaatgac gaaacagttg atatcttagg tgtagggata atttcatttt tcattgataa
     3541 aatagttaat aattcacttg ggacagattt tacttatctt gattttattg gagatgaggg
     3601 gaaagttgca aatggatttt ctgaaaaaat tattcaattt caagaaaata gaagtaaact
     3661 taattaagaa tcgtttacta tttaaacagt gtccttttcc tttttaaata tatacgataa
     3721 aataagtata tgaattgtaa aaggacatga tgattatgga agaaatagaa tggaataatc
     3781 tgactcaaaa taataaaaat aattctggat gtggaattat ctttatgttt gttatagttt
     3841 tgggaatagt agtactttta ataactgtca agatagtaga ttactttgcg aatactcctg
     3901 aagaacaaat acagagaaaa cttcctgagt ttgaaagagt accaccttat gatccaagag
     3961 ataattcatt tcaagtaaaa aaattaaaag agtctccaaa tgaaatgtgg tttgaagtta
     4021 cagttgaatc aagcgacttc aaagaaaaaa agaaagaata caattatatt gtaaaggcaa
     4081 aagtatcaaa aagatttatt tttaacccta aattagaaat atatagtgtt gtaatagaat
     4141 aatagtgtta aaaaaattgt aaaagttaaa gaagtcaaat tctgatgtga aaaataaaat
     4201 ataccatgga agacataaaa gaaaaactgg ataactagtt tatttaatgg cagaccgtta
     4261 aggcctgcca tttttatgta ttttagcgga ataaa
//
LOCUS       DACCSK010000169         4282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCSK010000169 DACCSK010000000
VERSION     DACCSK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.173"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            1..391
                     /locus_tag="IVP39_002645"
                     /pseudo
     CDS             1..391
                     /locus_tag="IVP39_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            391..1320
                     /locus_tag="IVP39_002646"
     CDS             391..1320
                     /locus_tag="IVP39_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587691.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1317..3053
                     /locus_tag="IVP39_002647"
     CDS             1317..3053
                     /locus_tag="IVP39_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7587692.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3116..3952)
                     /gene="pheA"
                     /locus_tag="IVP39_002648"
     CDS             complement(3116..3952)
                     /gene="pheA"
                     /locus_tag="IVP39_002648"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7587693.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPACLRALFRKQV
                     DLAVVPVENSLEGSVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIIGDRKMTSQQPAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(3949..>4282)
                     /locus_tag="IVP39_002649"
     CDS             complement(3949..>4282)
                     /locus_tag="IVP39_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7587694.1"
                     /translation="ESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAEA
                     DCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIIDT
                     IIERIKSI"
BASE COUNT         1329 a          866 c          771 g         1316 t
ORIGIN      
        1 ggaatcgaat tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc
       61 tctagctatg atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat
      121 cttttaaagc ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacca
      181 aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag
      241 aatctttcac agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa
      301 cacaccttgt tttttctgtt atataccgaa aaacggccat tcagttcaaa acaaggctct
      361 aaacaaaaac agtcgatcac ccattcattg atggatagcg acgattcgtt tactatttat
      421 cctgtttcta ccttttcgga agaatccggc tatgtaatca atactgattc caaagataca
      481 cttaggaagt ttgttcaaaa gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt
      541 tctgattctt ttgcaatcca agatatcaat gaaatattcc aagagatcag aaattgcacc
      601 aaaggacaac gtttctatca taagggaaag aaccttgctt atcagcatga acttcttccg
      661 ttattaacca accacgagca gcaagcaagt agttatttaa caaaaatcgt tcataaagac
      721 tacgctttag ctgtcaaaga acttgagaat ttcttcacga cgatattgga acagcctatt
      781 gatccagctt atttgaaaca tgaggcaatc aatcgtttct atgcattact caacgctttg
      841 gctgacgagc atgttgggaa tgaagaactt tctttgctca agatcgccct tccttcttta
      901 ttagccgatg ctgagtattt cgaagatttt tctattcttg tcaaagaaac acttgagaaa
      961 ctcacccact tttctgcaaa tgcaaaaaaa gacaatttcg atatcttgac tttgatctac
     1021 gagttcgtcc aggaaaatta tgagaaagaa atcaatttac aacttttatc cgaaaaatat
     1081 catttttcat atagctatct ctcagccatc tttactgaaa aatttggtat caacttttca
     1141 aaatatctaa aaaaagtccg tatcagtaaa gccaagctct ttttgacgga aacacggctt
     1201 actttgacgg aaatctgtga gaaagtcggc tataccgaac tgggttattt ctccagagtt
     1261 tttaaagaag agacaggaat gacaccttct caataccgta aaggaacttt tgtaaaatga
     1321 gaaaaaaaat caaagagcat gaactctttg ccaaacttct gttcattttg ttcatcggtc
     1381 tttttctgca agccatcgtc atttctttgt ttatttatca tcgatcaagg gatgcctata
     1441 tccagctgtt caatcaatcg aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa
     1501 gtttgaatga tacaatcgag aatacattag ccgcttttga ttctaatcca gctgtcaaaa
     1561 gctatttttc caatgatcca gcccaacata tggaacagct tcagcaacta agaacaatcc
     1621 aaaaaatgaa tgactctctt tcaaaaatcc atccaatgat cgattacgat gtattgatct
     1681 ttggagaaaa tggaagaact tttgtcggaa acgacatgct cactgccgta tctgccgatt
     1741 ctttttttca atctgcgatt gctcagagag tgaatgaacg tgctgctgat acacaaatgc
     1801 tttttgcaca ccacggattg actcttcgcg ataagaaagc acctagcgtc ttctttgtca
     1861 aaaaactgaa aaatacattg aatcacgttt acggatatgc tgttttatcg atctcatcca
     1921 agcaactagc gaatcttttt caatctgtag taaatcctga gatatcgaag atctccttca
     1981 tgaacgaaga acagacgatc atcgcatcaa atgaagaaaa tacaattggg agccgatcaa
     2041 tgattttcaa aaaactaaca gtaggtgaaa caacgatttc aaatggacag cagttgaccc
     2101 gtcttcctct gtaccgccag gattgtacat tactcagtca agtcgatctt cattctttat
     2161 ctaaccaaat gggcgtgatt tttccgatta ttttgtataa tatcgtgaca cttgcttttg
     2221 ctggctcatt ggtatttatg tatttggatc gacacacaaa atcgatctac cgcttgattg
     2281 attcgctaaa acggctagag gaagatggct taaaagctac tgttcctgta caaggaagcc
     2341 atgaagtcca attgctaggg aatacgatca atcaattgct aagtaatgct cagcatcatt
     2401 atgaacagac gatccaagac gaaaagaaga aacgttcgct tgaaatccaa actatgcaag
     2461 cacagataca gccccatttt atttataata cgctgactag cttgaaattt ttgatttggc
     2521 agaaaaaaac agatgaagca gttcaaggaa tcgacagttt cattacttta ctaagaagta
     2581 cgattgggaa aaaagaagaa gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt
     2641 acatcgatct tttgagtatg cgatatggcg aaagaatctc ggctaaaata atgattcctg
     2701 atgaactgca gtcattatct atgccgaata tgatccttca accaattatc gaaaacgcct
     2761 atcttcatgc ttttcacaat aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa
     2821 atcagcttct tttattcgaa gtgattgata acggcgatgg cttcgatact gaaaatacca
     2881 aacaaaaaaa tgatcatttt tcaggaatcg gcatcagcca tgtagatgag cggatacgcc
     2941 taatgtatgg cgatgcatat ggcttaacga tccaatcagt gattgggaca ggaacgactg
     3001 taacgatttg tctaccgctt cttcctaaaa atcaatcaga atatcaaaaa taaagaatag
     3061 ccaagcactg atttttttat tttaagttaa ataaatcaat cagtattctt gactttcatc
     3121 tacgcgtctc ttctactgtc agcactggat aacagcccaa tatttgactt tctcctccga
     3181 gcatcttgat ttcttggagc gcattctcta ctaatgtcat tgggcggtcc aacaacaaat
     3241 cgatcacgaa aaaatattct cccaaactgg ttttcaatgg tcgggattca atcttactta
     3301 agttgatctc ccgccatccg aaagctgcca gcattttatg aagcatccct ggccgattag
     3361 caggtagtgt caagatgaca ctcattttta ctggagctgg ctgctggctg gtcatctttc
     3421 gatcacctat gatccaaaaa cgtgtctgat tcaactcatt atcttggata ttctcagcta
     3481 agatctccaa tccaacatgc tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt
     3541 tcggatgttc tgctacatac attgctgcag cagttgttga ttctgttgct actaaaggaa
     3601 cattaggata atgcgtttct aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa
     3661 ggatttttgt aatctttgtt gctggattgc ctaaaagctg ctgcttgata ggaagaacga
     3721 tttcactttt aacttcgact tctggatgtt tggaaagaag atcgattgtg tgatgaacag
     3781 atccttccaa tgaattctct acaggaacga cagccagatc gacttgtttg cgaaacaaag
     3841 cgcgcaaaca agcagggatg gaagcatacg cagtcaattg ttctgtagga aatagctgac
     3901 aagccgcctg ataagtgaat gaactttctg gccctagata gctgacgttc atatgctttt
     3961 gatcctttct ataatggtat cgatgatctt ttctggtgtt tgattggtcg tgttgattcg
     4021 atgtgtcgca ctttcttcat accaagaaag tcgttgtgcc aataatgctt ttatttcttc
     4081 tcttgttttt ccaagagcta aaggtcgaat agacgtttca tcttgttgga tacgatcaac
     4141 tagacagtcc gcttctgcct ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg
     4201 attttctgca ctttggacaa tccctccacc agtcgcgata attttatctt catttgacag
     4261 ctttcttaaa atatctgact cc
//
LOCUS       DACCSK010000170         4232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCSK010000170 DACCSK010000000
VERSION     DACCSK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.174"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IVP39_002650"
     CDS             complement(37..1257)
                     /locus_tag="IVP39_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7587695.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IVP39_002651"
     CDS             complement(1277..1882)
                     /locus_tag="IVP39_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7587696.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IVP39_002652"
     CDS             complement(1931..2863)
                     /locus_tag="IVP39_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7587697.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IVP39_002653"
     CDS             complement(3284..4144)
                     /locus_tag="IVP39_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587698.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1202 a          855 c          694 g         1481 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acccgtaccg catctgttgc cccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa ga
//
LOCUS       DACCSK010000171         4112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCSK010000171 DACCSK010000000
VERSION     DACCSK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.175"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(124..1551)
                     /locus_tag="IVP39_002654"
     CDS             complement(124..1551)
                     /locus_tag="IVP39_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HAP7587699.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(1696..2244)
                     /locus_tag="IVP39_002655"
     CDS             complement(1696..2244)
                     /locus_tag="IVP39_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587700.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2245..3102)
                     /locus_tag="IVP39_002656"
     CDS             complement(2245..3102)
                     /locus_tag="IVP39_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7587701.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3388..3675)
                     /locus_tag="IVP39_002657"
     CDS             complement(3388..3675)
                     /locus_tag="IVP39_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7587702.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSVIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHEKFYEQLKKLI"
     gene            complement(3665..3994)
                     /locus_tag="IVP39_002658"
     CDS             complement(3665..3994)
                     /locus_tag="IVP39_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7587703.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         1233 a          786 c          608 g         1485 t
ORIGIN      
        1 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
       61 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      121 ttctcagact gtagacaaaa aggagtggct taattgccaa ctcctttttg ttagtctttg
      181 tagaaatttt aaataaaaag ccataggctt ttttgctttc catagccatg tggctagttt
      241 ttttaaattc atggcagcaa acgtaagcat cgtgtgcgtg gcgacttttt cgagacctcg
      301 atatttcgtc caacgcatac catgcttttc ttttgcatct gcaaatattc gttcaatggt
      361 ttgttttctc tttttataaa tttctctatt caacgccgta tggcgttggt gttcaagttc
      421 atccataaga tccgcccaaa catgtcgagt tactattttt tgatgttttg tactcaaagt
      481 acagttttta agtaatggac aactcctaca gaattttgga tttgatttat aggttcgata
      541 accatcacga gtgatagttg aaaataacaa cggttgatta ttgggacata aatagcagtc
      601 aaaataggca tcataaacaa agtctttttt acgaaacatt ccttttactc catgaggtac
      661 agtatatgga agaatcgggt agagtttcaa gtgtgacaga aaatgaacaa atttaggcgt
      721 cttatatcct gcatcagcga caacacgctt tatatttgga aaagctcttt tcgactgttt
      781 gaccagatcg attgcgactt ggctgtcatg tacatttcct ggtgtgatta atgtagataa
      841 gataaaacca ttatcttcac aggaagtatg cgcggaataa gcaaattgtt tttctcgttc
      901 acctttaaca tagtaaccac tttctggatc tgaatgactt attttttgaa gactcaactc
      961 actttctgtg tcccatgtaa aaggacgttt tcctacagca atacgttctt tatttatttc
     1021 atcttctaat tcatcttgat aagctttagc ttgaccgtag gtcatttctt ttgtgaactt
     1081 atgtttattg gcatttgcct taatatgagt agaatcaata taaaggttgt cttccgtaac
     1141 gaagccagct tttacagctt gttctagaat atgagagaaa atgtcttcaa ataaagttgt
     1201 ttctctgaat cttcgaacat agtttttacc aaaagtagag aaatgcggga taggatcctc
     1261 gaatgaataa cctaaaaacc aacgataagc aacatttgtt tcaacgtctt taatagtctg
     1321 tctcatcgaa cggataccaa ataaatattg gataaatact aatttaatta agaccactgg
     1381 atcaatactg ggacgaccat gggtcgaata cgtagattct acaatgggat aaatgaaatc
     1441 aaactgaatc gcagcatcaa tctttcgaac taagtgatct ttaggtacaa gatcatcaat
     1501 tgttaacata gcaaattgat ttctaccttc aatcgattgt tttctcatca ttagaatcac
     1561 tcccagaata gtttatacaa tgagtataaa acaaaaaaac tgcaaacaac actcccaatt
     1621 taggagtttg tctacagtct gagaagaact aagtaactac cgtttaaagg tagttactta
     1681 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
     1741 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
     1801 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
     1861 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
     1921 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
     1981 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
     2041 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
     2101 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     2161 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttattgt tcccaaaaat
     2221 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     2281 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     2341 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     2401 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     2461 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     2521 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     2581 catcgatttt acttgggtct gtaagacgac gattgcccag tccgcaatgt acatcgcgga
     2641 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     2701 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     2761 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     2821 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     2881 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     2941 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     3001 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     3061 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     3121 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     3181 gatatatatg tactacctta taattaataa ggaaaaggta gtacataaat aaggaaaagc
     3241 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     3301 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     3361 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaattt
     3421 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     3481 tctatatgtt gtgcccgcat atttaaaccc actagaccaa ataactgata aatccccagt
     3541 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     3601 ttctttcaat ggcttctctt ttagcttttt taaatactta atgacatgtt ttgaaggaac
     3661 gatttcataa gccaatttct gttgccaact cctctttagt cattacttta gaattagtaa
     3721 gtgtatcttc agcaatatct ctaaaggaag aatgcatttt gttttttcta ttcttaaatt
     3781 ctacaagaat ctcatcttta tcgtagcctt ctgcaattat atcagacaaa atatcttcgc
     3841 taaaatcata aaattctttt tgtggttcaa caaattcata aagaatacca ttctctacca
     3901 actttgcttc aacttcgact ccattaggaa cattaaaatc tttaggtact gtcaacatga
     3961 ttgaattgcc ttgttttctt gtttttgtaa tcatttaaat ccctcatttc taacagtatg
     4021 taattactta gtaaatacat tataccacga gataatgtat ttacaagtaa ttactgtgac
     4081 tcgtaatgcg tccttgctga ataaatttca ac
//
LOCUS       DACCSK010000172         3960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCSK010000172 DACCSK010000000
VERSION     DACCSK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.177"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(310..807)
                     /locus_tag="IVP39_002659"
     CDS             complement(310..807)
                     /locus_tag="IVP39_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7587704.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKAHQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IVP39_002660"
     CDS             complement(847..1656)
                     /locus_tag="IVP39_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7587705.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IVP39_002661"
     CDS             1990..2847
                     /locus_tag="IVP39_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7587706.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IVP39_002662"
     CDS             2840..3742
                     /locus_tag="IVP39_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7587707.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..>3960
                     /locus_tag="IVP39_002663"
     CDS             3775..>3960
                     /locus_tag="IVP39_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7587708.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATK"
BASE COUNT         1313 a          684 c          729 g         1234 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggtgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatatttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
//
LOCUS       DACCSK010000173         3956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCSK010000173 DACCSK010000000
VERSION     DACCSK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.178"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IVP39_002664"
     CDS             <1..156
                     /locus_tag="IVP39_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP7587709.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IVP39_002665"
     CDS             175..1452
                     /locus_tag="IVP39_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7587710.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IVP39_002666"
     CDS             1462..2118
                     /locus_tag="IVP39_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7587711.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IVP39_002667"
     CDS             2118..3494
                     /locus_tag="IVP39_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7587712.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IVP39_002668"
     CDS             3624..>3956
                     /locus_tag="IVP39_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7587713.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACCSK010000174         3923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCSK010000174 DACCSK010000000
VERSION     DACCSK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.179"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(573..1130)
                     /locus_tag="IVP39_002669"
     CDS             complement(573..1130)
                     /locus_tag="IVP39_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587714.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IVP39_002670"
     CDS             complement(1309..1920)
                     /locus_tag="IVP39_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587715.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IVP39_002671"
     CDS             complement(2033..2290)
                     /locus_tag="IVP39_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587716.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IVP39_002672"
     CDS             complement(2887..3165)
                     /locus_tag="IVP39_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587717.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IVP39_002673"
     CDS             complement(3199..3450)
                     /locus_tag="IVP39_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587718.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACCSK010000175         3897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCSK010000175 DACCSK010000000
VERSION     DACCSK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.180"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            415..1104
                     /locus_tag="IVP39_002674"
     CDS             415..1104
                     /locus_tag="IVP39_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7587719.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1091..2299
                     /locus_tag="IVP39_002675"
     CDS             1091..2299
                     /locus_tag="IVP39_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7587720.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2593..3864
                     /gene="serS"
                     /locus_tag="IVP39_002676"
     CDS             2593..3864
                     /gene="serS"
                     /locus_tag="IVP39_002676"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7587721.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRSVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1278 a          674 c          783 g         1162 t
ORIGIN      
        1 tttcgataga atcaagcttc gcaagtggtt cttttttaac gactcagtta tcttatcatg
       61 tcatttccaa catgtcaaca acttattttt tattatttaa taagttattg gtgttctttt
      121 gaacaaataa taatatatca acatcacatg tgaaagtcaa taagaaaatg cttatttatc
      181 aacatttcta gcttacatga tcattggtta gttctggtcc tttttcgttc gtctttataa
      241 ctccgagaga cgtaaaatcc ttttgttttc agtttaatgt tcatgttctt gtcttattca
      301 caccaactag atgtctcacc ttctaaaatt ttccttaaca aagtgctgct tttatctatt
      361 tctgcttttt tttgctagaa taatagtaac aatatgactc tggaggattt atttatgaaa
      421 attttgattg ctgacgatga taaagaaatc gttgaattat taagtatcta tgttcacaat
      481 gaggggtatg aagcagtaaa ggcttatgat gggaaagaag cattatctaa aatcagaaca
      541 atccctgata ttgaattact tattttagat atcatgatgc ctgagatgga tgggatgcaa
      601 gtagtgaaag agctacgaaa agaatcccaa ataccaatca ttatgctgac agcaaaaact
      661 actgatatgg ataagatcaa aggactggtc gctggtgcag atgattatgt gaccaagcca
      721 tttaatccgt tagaagtaat ggctagagta aagtctttat tacggcgtac aaaaatgcag
      781 attgccccag atcagcctga tgtattagaa gtaaatgcat taatcatcaa taaagactct
      841 catgaagtta aaacaatcga tgggaaagaa atccagttaa cagctttgga attcggtatc
      901 ctctacttgc ttgctagtca tcctaatcga gtatttagcg cggatgaaat ttttgagcgt
      961 gtttggaaac aagaaagtat tgtctctgct aaaactgtca tggttcacgt tagtcattta
     1021 agagataaga ttgaagaagc aactggtggt gaaaaagtca ttcaaaccgt ttggggagtg
     1081 ggctataaaa ttgacgcaag atagtaaacg agcaactgaa aaacgccgtc ggatcaattt
     1141 gacttcaaaa gaaatcagcg aattgttagc ggaaggaatc atcacgatta tccttttgct
     1201 tctattaaat gtttctattc tggttgttat cagttcggtg atcaacagtt caccttcttt
     1261 gacgaatgcg atttgggatt caaagaatat ttttgcagaa cgattgaaca ctgatttgtt
     1321 ctggaacggc cggaatttta ttattccctt tttctttcta ttagatattg gtgttttgta
     1381 ttggcgcttg attcgaagat atcgccaaat gcaattgcgg catatcatca gcgaacttca
     1441 ctacatcgca aacggaaatt atgatcatcg tatccctttt gaactgagtg gtgacttaag
     1501 ccgagtggta acaagtatca atggattggt ggatagtacc gtagcagcga ttgaagacga
     1561 acgtaaaatc gaaaaatcaa aagatgaatt gatcacgaat gtcagtcatg atatccgtac
     1621 cccactcacg tccatcattg gatacctagg actgatagaa gatggccagt atcactcaga
     1681 agaagatctt ttaaagtata cgcatactgc ttatatcaaa gcaaagcaga tgaaatcttt
     1741 agtggacgac ttatttgaat acactaaggt acgccagcct gctgtcccgg tgaatttttc
     1801 tgcctttgac atgatccagt tgatcgaaca gttagcagct gattttgagt tggaagcttc
     1861 taaaaaaaat atccaaatac ttgttcaatc aaaagtagac tcccttatca tggatggcga
     1921 taccgaaaaa cttgtacgtg tcttcaataa tcttttgacg aatgcgttga aatacggaaa
     1981 aggagcaacg aagatcgtaa tcgaagtaga acgtatcggt tcagaagttg ttgcaacagt
     2041 caaaaataac ggagcaatga tcccacaaca ggcaatcgat aatctattcg accgttttta
     2101 tcgtgtagaa gaatctcgtt cacaagcaac tggaggaact ggacttggtt tagcaatcgc
     2161 tcaaagcatt gtggctttgc acggcggata catttatgca aaatcagata agcaatggac
     2221 ttctttcatc atgcaccttc caatcaagaa aaatgaaaaa ttacctattg atacacagga
     2281 agttattgac gaaagctgaa atattcgcta ttcttattac aattattaga ataaatgaaa
     2341 aacacagaac ccagtacact ttattctttt tcagagagct gatggcaggt gtaaatcagc
     2401 aaaggataat ttggaagttc cagttcgttt acgtgccctt tcgaaataag aaaggtcggt
     2461 tcaacagccg ttatgtgttc aacaagtgca gctgacgctg aacttgggtg gtaccgcgaa
     2521 tttcagactg attcgtccca agaataatgg gatggatcag tcttttttta ttttcaaaaa
     2581 ggaggaaatc atatgttaga tgtaaaaatg atgcgacaaa atattgaaga agtacaagaa
     2641 aaattgatga ctcggagcgt aaaaaaagaa acattagctg aattcatgga actagatgaa
     2701 aatcgtcgta agctactagt caaaagtgaa gaattaaaaa aatatcgaaa tgatgtttct
     2761 gctgagatcg cccaattgaa gcgtgcaaaa gaagatgctt ctgctaaaat cgctgaaatg
     2821 aaagaagtcg gtggcaacat caaagcattg gatactgaaa ttgctgaagt ggatgaaaaa
     2881 ttgaaacaaa ttgcaactac tttgccaaac cttccccatg attctgtgcc agttggaaca
     2941 gatgaagagg acaatgtaga aatccgtcgt tggagcgaac cacgtacatt tgcttttgaa
     3001 ccaaaaccac actgggaaat tgcagaaaat cttgatattt tagattttga acgtggcgca
     3061 aaagtagccg gtagccgttt tgtctattac aaaggattag gtgctcgatt agaacgtgct
     3121 gtctacaact tcatgcttga tcagcatatt tacgaacacg gctatactga aatgatcact
     3181 ccttatatcg tcaacagtaa agcgatgttt ggtacgggac aatttccaaa attcaaagaa
     3241 gatgttttcc aattggagaa tactgatcta acattaatcc caacagctga agtaccatta
     3301 actaactatt ataatgatga aattttagat ggtgcagatt tacctattta tttcactgca
     3361 ctaagccctg cttttcgttc tgaagccgga agtgctggtc gggatacacg aggactgatt
     3421 cgtcttcacc aattcaataa agtagaaatg gtcaaatttt cggatgtaga gcattcttac
     3481 gaagaattgg aaaaaatgac agctgatgca gaagatatct tgaaaaaatt gcatttgcct
     3541 tatcgtgtga tggccttatc aactggagac atgggctttt ctgcagctaa aacttatgac
     3601 ttagaagtat ggatacctgc acaagatact tatcgtgaaa tcagttcttg ttctaactgt
     3661 gaagatttcc aagcacgccg cgcaatgatc cgttatcgcg ataatgatgg aaaaatccac
     3721 tatactcata ctctgaatgg ttcaggactt gcagttggcc gtacagtagc tgccattttg
     3781 gaaaactacc aaaatgaaga tggttctgtc acagttccag aagcgctcgt tccttatatg
     3841 gggaatctga aagttattaa ataaagaggc tttgaaaaag ccgcttagtt tcgagta
//
LOCUS       DACCSK010000176         3887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCSK010000176 DACCSK010000000
VERSION     DACCSK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.181"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            3..1085
                     /locus_tag="IVP39_002677"
     CDS             3..1085
                     /locus_tag="IVP39_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7587722.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1303..2445)
                     /locus_tag="IVP39_002678"
     CDS             complement(1303..2445)
                     /locus_tag="IVP39_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7587723.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2450..2635)
                     /locus_tag="IVP39_002679"
     CDS             complement(2450..2635)
                     /locus_tag="IVP39_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7587724.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2989..3234)
                     /locus_tag="IVP39_002680"
     CDS             complement(2989..3234)
                     /locus_tag="IVP39_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587725.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3228..3629)
                     /locus_tag="IVP39_002681"
     CDS             complement(3228..3629)
                     /locus_tag="IVP39_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7587726.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1289 a          695 c          586 g         1317 t
ORIGIN      
        1 atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac acccgaccag
       61 aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga caatactttt
      121 taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa tacgttaaac
      181 taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat catataaacg
      241 gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa aatttcatca
      301 atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac tctgtcctta
      361 agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat cctatgctgt
      421 atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta aaaatcattt
      481 ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct ttctatattc
      541 ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt ctgacgtggg
      601 atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta aagaatgacg
      661 gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt tttattggac
      721 aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat cgaatgaagt
      781 atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt gagctagtta
      841 cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc ctctctttta
      901 acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc gcaaaaatga
      961 aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat acttactcgc
     1021 atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt
     1081 cttgattttt gccaccgaaa aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac
     1141 atggtttatt ttttacactg ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg
     1201 tccattttcg ataccgacag ctagcttata acccaaggta tatcaacata aaaaaataaa
     1261 aataggctat gacagcagcg tgacagcaaa aaagatggaa agttaaatat caaacctcgt
     1321 aatttcattt gcgagtgctt gatttgtttc tggatataag tgtccatatt tgtccaaagt
     1381 agttttaata gaggcatgac ctagacgctt agatattgca tagatatttt tattatgttc
     1441 aattaaaaag gcaacatgcg agtgtcttaa tccatgcata tgaattggtt ttactcctgc
     1501 aatttcagct agttttttta ttctgcttaa aatagttctt ggagatggcg gtaatccatc
     1561 aaaagaaaaa accaattcta tatttttccc aattgattgt tgagtttttt tccatgtttt
     1621 taaatcttca acggtttgtc tgtctaacga aattatccga atacttgatg tattttttgt
     1681 ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt gacttattta cgctacatct
     1741 gccatttatt aagtcaaagt ctttccattg taacgctaag agctcctcac tccttacacc
     1801 tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct tcataaaaat ccccgtgata
     1861 aacactatta tatactttct taaattcatc aactgtccaa aattctactt gaggacgctg
     1921 agtttttatt ttacctatag tatctacagg attttcttca ataatttcaa gtacaattgc
     1981 ccttttaaaa actactctca acttattgaa tatctgttta atataatttg gtgataatgg
     2041 ttgctcaatg ccctttttta caacatggat tttcgataaa aactgttgaa attcctgtat
     2101 atgaattggt cgaatcttta caacctgcat ttctccaaaa aaatccaatg cacgttgtaa
     2161 aatatgatct gttttcatat atgttttttc tacagttccc aacttatacc aaggaaaaaa
     2221 atactctcga taaaaatcat gaaacgttat tccttgcaga taaattcctt gtgaatcaga
     2281 aaaactttgt tttaactctt cgagtcccct tctagcttct ccttgagttt tgaagcctct
     2341 tctggtcact tgaattcttt ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca
     2401 agtgcctctt tttctgtctt tataaacatt cgcaattttc ctcattttac tattcctccc
     2461 aagaaatatg gagaacttct tctaaaattg aaataggaat tctttttatt tttgaattac
     2521 gataaaaaaa gtatcctttc tcaactaaaa gttctttaca ttccttgaag attttattag
     2581 catagtactg agaaaaacct agattaatca aatcttttcg agtagctgtt ttcattatag
     2641 caccttcctt actttttttc atttatttct atcagctgct tttgtcaaaa gcacatagga
     2701 atttcacctc tccccaagtt tacggcgagc cttacggaag tatcattatc ccaactttaa
     2761 atcatcgctt tttattgact ataaaaatta ttttagaata tttatcgctc cttctttaca
     2821 gcaagaaatc atggcgcaac taaaatatgc tccttaaagt gttaacgtac tgatagaatc
     2881 tatttaattt tcaatgaaca ataggttttc tacctaaaag aatctactaa ttccttaact
     2941 attagaagat tcttttaaac agaaaaagtg gaaaggggat ttttttaatt agttaatttt
     3001 aaattttaag atcgcttcta ttaatttaat ttctagctga cttatcatat attcatctac
     3061 ataggtaacg aattgaccta cctcgttaaa tccatttgtc aaacataact tagccatata
     3121 agaatgataa tgtttgagta tcatcttgat cgaaattgga tctccttcta gagcagataa
     3181 gataatggat agtggaacta ataggtactg ccgcttttta gatttatcta ccataggtca
     3241 taccccattt tcaattgttc aactgccttc tttcgttgat accagatacc acttgtgctc
     3301 atattgagaa ttttaccgat ttcagcatca ttaaaacctg caaaataata aagtaggata
     3361 atctttcttc ttatctccga taactgatca attgctgctg ataacttttg atctgaaatt
     3421 aatatttcca ttccaaataa gaaatagaca tcttgttcat caaaatgagt ttgggaatac
     3481 ctgccttcat ttgaatctgc caatggttcc tcattatcct ttatgttagc gtttcgtttt
     3541 cgaatagact tagcttcgtt tcgtaatact tttttacagt aggcatcaaa ttggtgttgt
     3601 atagatatgt cattgtaatg ttgttgcatg ataatatgct cctttttccc cctttccact
     3661 aatacaacaa tcagaaagcc cctttttttc gaaaaaacta gaaaaaaaac ataaaaatat
     3721 ttatcattct ttaaacagaa aatctttcta taaaaaacaa aaaaaaacga ttaggctagg
     3781 ctaatcgttt tttgaacatt cttaaaaaat ccaaattatt ttttataact gaaattgggt
     3841 attgatgacc atcgtttctg tagatagtaa gcaatcattt taggttt
//
LOCUS       DACCSK010000177         3876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCSK010000177 DACCSK010000000
VERSION     DACCSK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.182"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(50..123)
                     /locus_tag="IVP39_002682"
     tRNA            complement(50..123)
                     /locus_tag="IVP39_002682"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Ile,seq:cat)
     gene            310..633
                     /locus_tag="IVP39_002683"
     CDS             310..633
                     /locus_tag="IVP39_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587727.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            797..1051
                     /locus_tag="IVP39_002684"
     CDS             797..1051
                     /locus_tag="IVP39_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587728.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            1121..1366
                     /locus_tag="IVP39_002685"
     CDS             1121..1366
                     /locus_tag="IVP39_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587729.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1442..1807
                     /locus_tag="IVP39_002686"
     CDS             1442..1807
                     /locus_tag="IVP39_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587730.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1868..2431)
                     /locus_tag="IVP39_002687"
     CDS             complement(1868..2431)
                     /locus_tag="IVP39_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587731.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(2999..3199)
                     /locus_tag="IVP39_002688"
     CDS             complement(2999..3199)
                     /locus_tag="IVP39_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7587732.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1168 a          680 c          606 g         1422 t
ORIGIN      
        1 ttggctgttc ttattacttg atgcagggca gctttttcac aacctcttat gtaccttgta
       61 ggactcgaac ctacgaccgg acggttatga gccgtctgct ctaaccaact gagctaaagg
      121 tacatcataa ataaaattta tttttgttac ttttcaacta tagcacgaat gacaaactct
      181 cacaagtttc aaaatataag taaaataata ttgttattct tatttctttt acttgtgtca
      241 taactatttt gttactttaa tttttatttg cttattttag aaaaaatgtt attcttttat
      301 ttattctcaa tgttaggagt gattattttg ccatttattg cgattttatt cgatttagtt
      361 gcggcagcag cttattttct ccaattaaat tatcaaacct caggaattct gtttattgga
      421 atgatttttc aaggtgtgat tacccttttg cttcttgtca tgatgctcag ctataaagga
      481 aaaagatatg cccgtattca aacaaaagta tttgttaaat atgtttccat tcgctatggg
      541 atcatcattc tttccttttt agtgaatgcg atcgtcctct ttttatatgt attgaattac
      601 ttagatatca atccattgat tttttctcga tagataagac acattcagcg gaacagttcc
      661 tgcttatcag cagaaaactg ttctgctttt tcatttttca ttgatctatt cttcttttat
      721 tttcctagca tttcatttgt gatgtatgca ataacatgtt atcttttatt atatgaatcg
      781 ttgaggagtc cgttacatga ttacgttggt gagagttttg ttttgggtgc ctgcggtcgc
      841 actggtcgca agtattgttt atcttatgaa ttggaacaaa gaacggtttt atttagctat
      901 tttgactctt ccggctatct actttatgtg gaaagttttt aattacaact actttgaacc
      961 cgatagtgtt tttatagaag aattatcagg actggttttg tctttattga tcgtcattct
     1021 ttatttgata cgtttaaata aaaaacattg agtactattg attgcatgtg tagctttcta
     1081 tagtcattat aactattcta ctataatgaa aggtgattaa atgagtatcg aagaagctat
     1141 tttactggat actttacgtt gtcgtctgaa tttcttaaga actggccgat ctttatctca
     1201 agtagctgtt tctagcgcgt taagtattcc tttattcaaa tatcgtaagt acgaagaagg
     1261 agaagaaatt ccaagcatca ttgaattaat ctatatcgct gacttttacg atatttccgt
     1321 tgattaccta attggtcgct cagaaacagc aagcaaactg aagtgatttt ttccagcctt
     1381 ggtgctggtt ttttcattta acgtctaagg ttaacaatct atatataaag gagacatttc
     1441 tatgcgatta tggcatgaaa aattgatttc aaagcttcct cgtcaacagc ttttaggaca
     1501 acatcgggaa tgctgtgcat taagaggaaa tggctgggga aagaaacatg ctactgtgga
     1561 ttatgttttt cactattctc cttatagatt attccaatat catcagcttg tcatggatga
     1621 aatggaaaaa cgtggttaca gcccagcacc agagtggaaa aatcctatgt atcgtggcaa
     1681 atcatgcgaa gcattttttg atttacctac tgtagagcgc aacgtgccga tttatccgga
     1741 acacgatgaa gtttatctag cagaatgttt agcaaatttg gagcaaaaag gaatttatct
     1801 tacctaatat aactagaaaa gccttctatc tcctcttact tcattaaaaa gctttaacca
     1861 tcttagatca agctaaaatg tcgtaagttt tagggttatc ttcatcctca cgtgcaaaac
     1921 taaaagcgta ctctagtttt ggttcatcgc caacttccct agacataaat gaacatctcc
     1981 tgacgtggtt taaaagtacc gcatcttcga cgtctgtata cgatgcacag cacagattgc
     2041 ttcttttgat ctttcttttg atttttctgt tgcttcttct acaccttctt cgattactac
     2101 atcgatgctt acaattgctt ctgcctaata ttcccatgca tcgtcgattt aagcctctgt
     2161 agtcactgtg atcgtttcca cattttaact cctctgtcgt ggcttcagtt gattttgtga
     2221 tcagttcagt tgctaagcca gagtcagaac aacctgccat tgctagcgtt aatattgata
     2281 gtaaaaatag tttttttatc ataaggttcc ccccctgaaa atagactaaa ataataacgc
     2341 tttctttttt tgttttcaga cgaaaattaa taatttctaa atattcttta aaaaagctta
     2401 taactattat gcctttttta atatataaca cagttatttt taaaaataag aatgctaaat
     2461 cactctacaa taacggcgaa agcacaaaaa ataaaaaaat tattacgtta ttaaaaaaac
     2521 agatgaaaga tcaggtttta ttagatctca ttctttcatc acttgaaggg atttaatgaa
     2581 ctactttttc ctctttgtcg tttttatata ttcgtattcc ttcaaacagt aggatagcct
     2641 ctttcaatgc ttgatatttg aactgtccgt tacttaaaaa tttagatgtg ccgccctctt
     2701 cctagaacat attctttttt actttttcat taattgacag accaatcgaa cgaaactgta
     2761 aatttcgata ggataatgat tggtcaaact cattcatttt caagacatct cgattcatat
     2821 tcaagatagg aatcttttta tcaacatcac tttattcgta ttttcctcta ccataaaagc
     2881 ttcacgctcc acaaaattgt tttatacact actattcagc ccattcaacg accaatgaat
     2941 tttttcgtac aaaaaaaccg tcttttcttt cgaaaagacg gttaaaagtc aatttttatt
     3001 aagctttaac aatgtttgtt gcttgagggc cacgttgacc atcttcaaca tcgtaagaca
     3061 ctgcttggcc ttcttctaaa gttttgaagc cgtcaccttg gatagctgaa aaatgtgcaa
     3121 atacatcatt tccatcttca ccagtgataa agccaaaacc tttgtctgcg ttgaaccatt
     3181 ttactgtacc gttattcata aataaattcc tcctgatacg tatcaatgta cgttttttcg
     3241 ttgcaataaa tatttgatag gcaaaaggag ttttatctat gtagatatat cgctcaaatt
     3301 acgtttcaaa atagattact catttaatat atcatgcttt ttctaataac acaaggataa
     3361 tgcaatgaaa tataaaattc attgtcttct attgtcttcg aatcgaagaa aaagttgaat
     3421 aaccgcgatt gattcacttt ttgttttctt gtttatttgc ttttatctgc aacttttgac
     3481 tttaaaattt cttaactatg aaaacctttc aattggtggt atacttataa atggaatttc
     3541 gttatcaacg cgttcgttgc ctttcagcaa gtatttaaag aaaggagaat ggctatgaca
     3601 ttctataaca aagatataca atatcgagta accttggata cagatttgaa tctatttgtc
     3661 gtttttgaca aagaaaatac caatcatgta gcgacaggtg ttacgatcga acaagcagtg
     3721 caagaattaa aaaaatctgc ataaggagtg gaaaccctca tatggacgat tctcgttctt
     3781 ctctttaaac gcatctactg gtagataaca gtagatgcgt tttgtgtttg ttctaaattg
     3841 ttcactacaa ataaggtaga tacttcaaaa taaaaa
//
LOCUS       DACCSK010000178         3732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCSK010000178 DACCSK010000000
VERSION     DACCSK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.184"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IVP39_002689"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IVP39_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7587733.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..>3732)
                     /locus_tag="IVP39_002690"
     CDS             complement(3250..>3732)
                     /locus_tag="IVP39_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7587734.1"
                     /translation="EINRTYTKYILEFETIPEELKDKRVEEVDRTPAENLAYQVGWTN
                     LILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNRNIDDIN
                     MMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWKRLAL
                     "
BASE COUNT         1213 a          605 c          577 g         1337 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tc
//
LOCUS       DACCSK010000179         3597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCSK010000179 DACCSK010000000
VERSION     DACCSK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.185"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..122)
                     /locus_tag="IVP39_002691"
     CDS             complement(<1..122)
                     /locus_tag="IVP39_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7587735.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGI"
     gene            197..475
                     /locus_tag="IVP39_002692"
                     /pseudo
     CDS             197..475
                     /locus_tag="IVP39_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            618..839
                     /locus_tag="IVP39_002693"
     CDS             618..839
                     /locus_tag="IVP39_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587736.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            855..1697
                     /locus_tag="IVP39_002694"
     CDS             855..1697
                     /locus_tag="IVP39_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587737.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1760..3562
                     /locus_tag="IVP39_002695"
     CDS             1760..3562
                     /locus_tag="IVP39_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP7587738.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1292 a          510 c          727 g         1068 t
ORIGIN      
        1 ttaatgccac gatcatataa gatttcttga acttcacgat agctaaggtt ataacgaaga
       61 tagtagccca cggctacaat aatcacatcc tgctgaaatt gctttccttt aaaatgattc
      121 atcgtcattc ctcctgctat ctttttctat tattctacct tatttgatag tagatttaaa
      181 actttgcaac agaaccaaag aactcatcaa gctggctaat aagggtcaag attatgaaca
      241 tctagcagat gagattgatg agctgagaga caagcgacag ctccttttag tagaagatgc
      301 ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt gaatttatcc gcaagaacaa
      361 attccgtacc ttagagtacg atgataagct tgtaaggaag ataatccaga gcgttacagt
      421 ctatgaagaa cacttcgtca tatccttcaa atctggcatc gaaatggaaa tatgaaaacc
      481 agaagtaaat agcctatgat tctacagtag agtcgtgggt tttcttttgc ttgctgatta
      541 taataatatt ttaataacta ttgttattat aaaccaaatg atttataata ttcttagcgt
      601 aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt gagcaaatga
      661 atataagtgt ttccgaactt gctagacgta ttggacagac tccacagaat ttcaataaga
      721 aattacaacg agaaacggta accttagatg agttgaaagc gattgctgat gtgctaggtg
      781 tcaagtttgt gcaggctttt atattgcctg atggcaatga aataaagata ggtaattagg
      841 aggtgtaatt tgtattgaaa atcataacaa taaatgatgt tgagtatgct gtatttgctg
      901 ctaatgaagg tacttccaaa ccacaacctc atatcataga aacgaaatct ggtactattc
      961 cagaaggaaa acaactgagt ttattaaaag aatatcttaa gcagaatgat atttccccaa
     1021 taaaaggagc aacaacatat tggtgcattg acaaggttct taagcttgat tcaagtaaag
     1081 aaaaaactat aagtgaaaca atccataaac aaaaatactt atctttaact gaagaaaata
     1141 ttgagaaaca gcataagttt gtaggtgcaa gctctaatta tgggaaagaa ggtctaataa
     1201 tacatgatgt tttgaatgca tttcctcttc ataatgattt aaatactatt gcgatgaaga
     1261 ttgcggtaat tgatgtaaca aattcaacac atctttctca atataagagc aggttgtctc
     1321 tatatgattt ggctaaagta attctggaga ttccaaattt tgatgatcgg ttggcaaaag
     1381 gagatcctca gttgataaat attatcgcaa ggaacattgg tgcagttaat atgttctcat
     1441 ttgcatccaa gtactgtaca tatcataatg tggaagtgtg tgggagagat gattactcca
     1501 tctttgatgg tatcgttaaa aatactcttc cacactatat ccaaggacta actacgaata
     1561 agattgatac gtggcgaaga agttttgatt acgaggcatt taacgaatgt gttggaaaac
     1621 tacttgatga aaataatata catattcctt ttagaagaag aaagttagat cacttcttgt
     1681 ggtatgcgaa taggtagaat ataagaaaaa ggcatcgtaa aagtgattct ggataaaatt
     1741 aatgggggag gaaataatag tgggattatt tgattttcta aagaaaaaac aaggaaaggt
     1801 tagtaaacac gttgattatc aactggcttc tagtctattt cgtactggta aagtttttgc
     1861 tgaaaaattg gaagtggaca tatcaaatgt agatgagttt cgtaaaactt ttatcgcttt
     1921 tgatgtggaa acaactggcc taaattcttc ttcagataga attgtagaga ttggtgcggt
     1981 gctatttaaa gaaggagttc caattcaatc attcggaact cttgtcaatc caaaagttag
     2041 aattgcagcc tcagcttcgg caataaataa tataactaat gaaatgctaa actctgctcc
     2101 aaatgagcag gaagtttacc cccagttggt agaattttta ggagatgcac tagatggaaa
     2161 aatcactatg tgtgcacaca atgcaagttt cgattttgga tttttaagta acacattttc
     2221 tagacttggg tatgatgcta aaattaagta tgttgatact ctgagtctct ctagaaaaca
     2281 tataaaaggc attgtaaatc ataagcaaga gactgtagcg gaatatttcg gattaataaa
     2341 tgacactgcc catagagcgg aatcagatgc agatatatgc ggaaagattt tttggggtgt
     2401 tttagatatt attgaggagt cgttggaaga acaaaaaaag caaatagaaa agatgactcc
     2461 agacataaaa gaacttgaag cttgtgcttt tattcaagat attattgaga aaaaaggatg
     2521 cgacaccagt tggatcaggt atcgaaaaaa cagtaataat tatgtcgatg taacttgtct
     2581 atatacattc ttaaagttta agtttgctaa gaagggaaat tacatcatta ttagaaaaga
     2641 tgctacaaat ggaattgatt tacctataga accttgtact gtaagtgaag gcggtacaac
     2701 aaatgtgcga gtctattttc gtagcccctt tgacttaacg cctctttcaa attatatttt
     2761 cagtgcttat agcgattgtt ataattctat gaatgaatat atgagtgtta gtaattacac
     2821 taaaaaagaa gcagagcaaa gccttagtat gctgaaatct atttcaaaat cagcgatgaa
     2881 agagttatta ttcgatgcaa aaaatcgtgg atatgatgag actgaagtta acctaaatat
     2941 tgaaaaagtg atatctagag aagatgtaat tattaacggg ttgcataatc gagtttcatt
     3001 aaacaagata cgaaatatag ggaattggga gaaaggattt gatgcaggtt atccatattg
     3061 ggaacgtgga gaaactgcta ggaaaaatgg caaagtagat gaagctattg cactttttga
     3121 taaagctcga tataacggct atgatgctcc tgcgctatat gattcttatg ctaaagctta
     3181 tcgaaaaatt aaggattacg ataatgaaat tctcattctt gatgaaggaa ttatgcgtaa
     3241 aacacggcac gatgttggta ctttagaggc tcgtagggat aaggcaatta agctattatt
     3301 tgctaaacaa gaggcagagc ggatagctaa ggaaaaatct gattttctaa aagcgaacaa
     3361 aaaagaggat gcgattagtg agcctagcaa gaagcgtgga agacagatta ttcaaatgac
     3421 tgatgatgga acaataataa aagaattcga tactatagct tctgctgtaa aagaagttgg
     3481 cgttaactca aaaagtatta gggatgctgc aaatggagtt caaaaacatg ctggaggata
     3541 ctactggaaa tacaaagatt agcagataat ctaagataac ctaggatctt cttaata
//
LOCUS       DACCSK010000180         3550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCSK010000180 DACCSK010000000
VERSION     DACCSK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.186"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(320..805)
                     /locus_tag="IVP39_002696"
     CDS             complement(320..805)
                     /locus_tag="IVP39_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7587739.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1285..1926
                     /locus_tag="IVP39_002697"
     CDS             1285..1926
                     /locus_tag="IVP39_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7587740.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1945..2580
                     /locus_tag="IVP39_002698"
     CDS             1945..2580
                     /locus_tag="IVP39_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7587741.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2598..3413
                     /locus_tag="IVP39_002699"
     CDS             2598..3413
                     /locus_tag="IVP39_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7587742.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1164 a          578 c          751 g         1057 t
ORIGIN      
        1 actctcatct gtgctgggat attcttacaa aaatataaaa aagtttagtt agaatataat
       61 attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa
      121 gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat ttggctaact
      181 atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt gtttgattgg
      241 ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg aattgggaaa
      301 taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct aagtaaacaa
      361 gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata tttggatgaa
      421 agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt tttttctctt
      481 tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg aggtcaggaa
      541 gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct agttgtttta
      601 aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct tttcttgatt
      661 tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt ttgactgacg
      721 taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt agttcataag
      781 cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt agtgtatcat
      841 aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt aaaacaaatg
      901 acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg gtggaaagag
      961 gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat agtaggaaaa
     1021 tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc tggcttttcc
     1081 gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat cttcggatca
     1141 aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa
     1201 aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca agaggatttt
     1261 tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc gcaattatta
     1321 gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc attaccatta
     1381 gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta cttgcttcaa
     1441 acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt tattttttat
     1501 gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat tgcctttatc
     1561 ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc gattcctcaa
     1621 ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc
     1681 gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt tatcaacttg
     1741 gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa
     1801 atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat catctatctt
     1861 acattgactg gtattctaac aatcgtaatg aaacaagttg aaggaaaatt aaattattac
     1921 cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa agcatttggt
     1981 gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac attagctatc
     2041 gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg cttagaaagt
     2101 tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga gcttaaaatg
     2161 aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca tctgaatatt
     2221 tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga agaatacacc
     2281 aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa tatctatcct
     2341 tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt ggctatgaat
     2401 ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt acgccagcaa
     2461 gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat tgtcacccat
     2521 gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc aattcattaa
     2581 gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc gcaggtcttc
     2641 ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg actatcaacg
     2701 aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga tttagagata
     2761 aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt gaacaatatg
     2821 ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg
     2881 gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc
     2941 aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa
     3001 tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg tcatctggtt
     3061 atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac caagatgctg
     3121 ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg gtcgatggtc
     3181 tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa ctagaagact
     3241 tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca cgcaagtcag
     3301 atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa acaggtgaat
     3361 tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat taacaaatag
     3421 gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg ttctcctgcc
     3481 aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa
     3541 aagccagttt
//
LOCUS       DACCSK010000181         3488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCSK010000181 DACCSK010000000
VERSION     DACCSK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.187"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            589..969
                     /locus_tag="IVP39_002700"
     CDS             589..969
                     /locus_tag="IVP39_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7587743.1"
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            951..1865
                     /locus_tag="IVP39_002701"
     CDS             951..1865
                     /locus_tag="IVP39_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7587744.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            2105..2794
                     /locus_tag="IVP39_002702"
     CDS             2105..2794
                     /locus_tag="IVP39_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587745.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GFIFYLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2915..3001)
                     /locus_tag="IVP39_002703"
     CDS             complement(2915..3001)
                     /locus_tag="IVP39_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7587746.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         1272 a          621 c          689 g          906 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggtaagag tatgaaaaat tttatgatag acttatttat
       61 tctttgcaat tgcccaaaaa attaaaccgt caattacaaa agacttatga aactaaatca
      121 actgaagaat gggagagtta ttgctagatt tttggtagaa gtaacaaaaa gtggacatat
      181 tatcataatg actcgccgaa agtctgataa aaaattggct aaaaatattg ctgaatgttt
      241 aataaaacta tcagttttag ctaaatagtt agtagtttac acaaaaaagc atttatgttt
      301 tttgttgcaa agcaacaatt tcatcttcgg tgaaataagc gaaactttac aaaaagtggc
      361 tagcatttgc tagctttttt tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg
      421 agcttgcgcc cctttgacaa gcggttattt gcaccaaata cggaggtatt tggctagcaa
      481 atatagagct tgccaaaccc ttttagtgag ctttactcat tgtacctttt taggtacaat
      541 ggtattgcga acgatacatc cgtaggacgt actgaaaggg tgggacttat gagcgaacaa
      601 aatcaaaatt tggctagcaa ttattctaaa aaatatacct atcgatccga gccaaaacaa
      661 atcagttttc gagtgagcga atccgaattt gcaaagctga agcaatcggc tgaagctttg
      721 caaatgagtg tgccggcttt cgtgaaagcc aaggcacaaa acgcgcgtct ggtgacaccg
      781 aaagtggcgc cagacattgc ccaggcaatc gcgcgcgatt tagccaaagc tggagggaat
      841 atcaatcaaa ttgctaagtg gtgcaataca caccaacatg acgttgcttc tggtgacgct
      901 caacgtttat ccgaaaacct aaaaatcatg caaaaggagc tacaaaagat atggcaacaa
      961 ttaaggtaag ccgaaccact tcttgtagtc gtgcaattaa ttatgcagaa ccacgagcaa
     1021 ccattaaaac aggaataaac tgtgacgtcg attatgccaa aagtgaaatg aaacaaattc
     1081 gcatgctgta tggaaaagac gaccgtgtac aggcacactt attgattcaa tcttttcgac
     1141 caggagaaat taccgctgaa aaagctaatc aactaggggc agagtatgca aaaaaaatag
     1201 cccccgatca ccaaatagct atttacacac atacagacaa agaccatatt cataatcaca
     1261 ttgtcattaa ctccgttaat ctggagactg gaaaaaaatt tcaagctcat ggtcaagcat
     1321 ttctaaacaa atgttatgac ctaaatgacg agatttgtct tgcacataac ttaccaatca
     1381 ctgagcgagg aaagaagcca gaaaaacgaa ccatgtctga aataaaatta aaagaaaaaa
     1441 atgaacctgt ttggaaagac gaaattcgga ttgcgatcga tcaaacaatg aaaaacaaaa
     1501 aaaccagaac ctatgaccaa ttttgtaatt ccctcaaaac attggggatt cattgcttca
     1561 atcgcgggaa aaatttcact tacgaattga tccacaaaaa gaaaaaagta cgatccaaca
     1621 gactaggaaa ggactacgaa aaggagacga ttttacatga gctggacaga cgagaacgaa
     1681 cctataacca acagcgaacc aacgagctac gaagagctcg agctagcatt cgcgaaactg
     1741 cagaagaagt acgaagagaa caacgaactc ttacgcctga acaaccggaa attagaccgg
     1801 ctcgaagaaa ccatgaacaa cacgttgaaa gaggaacaga ccaccaacaa gaattatcgc
     1861 tctaaaacgt taaaagacat agaaaacgaa ataaagaaac aacgtgcttt tctcgttcct
     1921 tatacgcaag aaaggataca aactacgatt gatttacaag ttaaaaaact ccatcaagag
     1981 ctattagacc agcgaaataa ggaacagaaa aaggtgctta ttcaacgatc attggatttt
     2041 cttgccccct tatgttgctt agtcctgaca ctgttcgcat tttggttagt atggggaggc
     2101 taaaatggca ttttatttac cctatctact catttttgtt agtatttcag gaagtatttg
     2161 gctcatctat aaaatttttc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa
     2221 gcgatttttc tttcttggct ttatatttta cttgataatc gtttcaagcg gcttattcgg
     2281 cgttttagag ggaaataagc gcgtttctag aagtatttta ttaggaaatg tcactcaaaa
     2341 gtatgaatca gcgcgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggaaga
     2401 gtttaccact tgttatgagg aaatgaacga tatttttgtg aatcaagaaa aacgtttgac
     2461 ggataaaaac atggaaaaac tcactcgtct ttatcaaaac ttgccagaaa aacagcaaaa
     2521 agaggttcaa gacaactatg agcgaaccaa aaaagacgtg caatatgtaa aagatacaaa
     2581 gatagaagaa gcctgtagtg atctatttgg agacacaaac ccttggtttg ccagtgaaga
     2641 agaaaaaaaa gaaaaacaac aatctgtcac atatgaacgc tatgaaatct tgcttcaaca
     2701 agcaactaac atgcaaaatc cgacaaaaaa agaaaccgcc ttaaactatc taaaaagtgt
     2761 caaggaatgg ttagatcagc aacaacaaaa ttgaatttga tttaaaatca tgttaaaatg
     2821 aatgtgcaaa tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg
     2881 gctttttttc aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc
     2941 aataactgaa taaagagaca caaactccta caagaagggg gcaaatgaac agttcaaaca
     3001 ttagatacac ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatatctta
     3061 aaaaattaaa tttgtgtggt gtttttaagt atcacttgtt ttagggttgt tagggcttgc
     3121 cctgaccgcc tgtaaggcgc ttctcgacta gattaaggga attaatcaag aattatctta
     3181 gaacggctta gacaagcttt cagagcctca cacctcctct ttgaacaacg gttttagcaa
     3241 aggagccgga ggcgactgcg cagtataata tgagagcata gcgatcacac ctttttagcg
     3301 accgtaggga gcaagttttc cagtttagaa aaattcgcga acctttttgg aggaaaaagt
     3361 aggagttttt taggcgggaa attcaatgtg cttttaagat atactgttag atatactgtt
     3421 agatatactg ttaataataa ctgttaggga ttagcaacct tatgggagag taagtattga
     3481 cataggta
//
LOCUS       DACCSK010000182         3478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCSK010000182 DACCSK010000000
VERSION     DACCSK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.188"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            1..999
                     /locus_tag="IVP39_002704"
                     /pseudo
     CDS             1..999
                     /locus_tag="IVP39_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1100..1672
                     /locus_tag="IVP39_002705"
     CDS             1100..1672
                     /locus_tag="IVP39_002705"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7587747.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(2145..2717)
                     /locus_tag="IVP39_002706"
     CDS             complement(2145..2717)
                     /locus_tag="IVP39_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7587748.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2733..3338)
                     /locus_tag="IVP39_002707"
     CDS             complement(2733..3338)
                     /locus_tag="IVP39_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7587749.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1141 a          672 c          582 g         1083 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtcaaaa aaactgttgc cattccatca tttcaaaaga
     1141 gaatctctta aatcaggact tttctactga aacgatctgt gagaaatggg cagctgacat
     1201 tacgtacatt cctactaaga aaaatggttg gtgttactta tcttcaatca tggatttaca
     1261 cacgaaaaga attattagtt atacattttc aaaacgaatg actgtggatt gtgtcattca
     1321 aacattgaat aaagcaaaaa tacattatca cattccagaa ggaatgattc tacacactga
     1381 cttgggcagc caatacacag caagagaagt ggaacaatgg cttaaaacca acaaaataag
     1441 gcattcttat agtagaaaag gaacacctta tgataatgct ggaatcgaat ctttccatgc
     1501 ctcattgaaa aaagaagaag tctacacgac tagctactca gattttgaag aagcaaatcg
     1561 agcgctattt agctacattg aaggatttta taaccgaaat cgaatccata gctcgattca
     1621 ctatctaacc ccacaggaat ttgaagaatt agcaaaagaa aaaatggctt aaccgtctct
     1681 actaaaatgt gtccaagata ttgactcaaa tccagtaata tcacaatagg aatgctgttt
     1741 attatattag cagcagtgct gatttttatg atagttattt ggttcttaaa tgaaagatct
     1801 aaagacaatc aacaccgtag ggatatggag ataaaatcaa tagagtcaat agattattta
     1861 tcgataataa cactaaaaaa gccacgttcc taaacgtgac ttacgctgaa cgatatgtgt
     1921 tgtttggtag acgaacaaat cgttcattat aaatatatga tggtatccta atatagtaag
     1981 cgtggctagc atacctttaa ttactttacc ttttacagtt accatacaca ttttttgagc
     2041 agaaatcaag taaataaaaa aatattatat ttcacaacat gctaatagac tcatatctag
     2101 aacgcattaa gtatttacct gtctggttcg ctgagacttc ttttttaact ttcttcaatt
     2161 ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg ttatagcttc aacttcctta
     2221 tacgatttac ctgaagtaag cagttcatat gcgtgcctct tcttcggtgt gattgttgct
     2281 ttcggacgtc cttctctgaa attaggatta ttctttcttg caaacatttt tccttcttgg
     2341 gtacgttcca caatcatatc acgttccatt tctgctacac tcaacaacgt ccgtgtcacc
     2401 attcgcccca tggacgtatt ttcaatcgtt cctagattca aaaccttgat cgttacgtta
     2461 tcatcgagta aaggttcaat gatctctaat gcttctctcg tgttacgagc aaatcgatca
     2521 agcttcgtaa caactatttc atctccaggc ttcagaccag ccaacagctg attgaatttt
     2581 ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa tattcttctt ctctacgcag
     2641 ttctcaagca actgttgaat ttgttctttt gtactttgag aagcggttga gacacgtgca
     2701 tatccatatt tcgtcatttg gtccacctac ttttacaaat catctatcgt atactcagaa
     2761 tacccttcat agaaatagct gacatcgatt cctttcatat ataactcgcg atcgtcaatt
     2821 ttatcagtca gagcattgga aattagatag cgtatttcca aatcgtttat gggacttcgc
     2881 tccattgcag aaagatagtc tgctttatcg attaagttcc aatctacaac tttttgcaat
     2941 tcttccttta aaatgagatc cagccaaatt ctagtacttc ggccatttcc ttctcgaaat
     3001 gggtgagcga tattcatctc cacatatttc ttgataatct cttcaaaaga agtttgtggc
     3061 atctgatcaa tatgctgtaa cgaatgttct aaatacataa ctggtgcaaa tcgaaaattt
     3121 ccttttgcaa tattgaccga tcggattttt ccggcaaaat cgtaaatatc cgaaaataga
     3181 aattcatgaa tttcagataa acctttaaat gtaccaactt cgatttcatt tatttttcca
     3241 caatcgtaca attctttcgc tttttgttta ctgagttttt cttcaacctt agccaactca
     3301 acttgattgg ttaatcccaa tttattctct aaaatcatgc gaaatctcct taactttatg
     3361 acataagttt atgaaacact ctcaacccca ttatatcgcg attttgttta tgtgtcaaaa
     3421 actattaagt ttctgacccc cgatagattt ttcaagtgtc tctattttgc acagtcta
//
LOCUS       DACCSK010000183         3376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCSK010000183 DACCSK010000000
VERSION     DACCSK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.189"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            254..580
                     /locus_tag="IVP39_002708"
     CDS             254..580
                     /locus_tag="IVP39_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587750.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IVP39_002709"
     CDS             complement(697..1041)
                     /locus_tag="IVP39_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587751.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IVP39_002710"
     CDS             complement(1117..2364)
                     /locus_tag="IVP39_002710"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7587752.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IVP39_002711"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IVP39_002711"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7587753.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          666 c          671 g         1052 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agtagt
//
LOCUS       DACCSK010000184         3315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCSK010000184 DACCSK010000000
VERSION     DACCSK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.190"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVP39_002712"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVP39_002712"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7587754.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IVP39_002713"
     CDS             complement(2260..2511)
                     /locus_tag="IVP39_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7587755.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IVP39_002714"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IVP39_002714"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7587756.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          912 a          726 c          616 g         1061 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaaga
//
LOCUS       DACCSK010000185         3133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCSK010000185 DACCSK010000000
VERSION     DACCSK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.191"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            208..1041
                     /locus_tag="IVP39_002715"
     CDS             208..1041
                     /locus_tag="IVP39_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7587757.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1143..2843)
                     /locus_tag="IVP39_002716"
     CDS             complement(1143..2843)
                     /locus_tag="IVP39_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587758.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
BASE COUNT         1110 a          520 c          623 g          880 t
ORIGIN      
        1 gaagcagcag tttgaatact gtttagtgga gaggtcgtga aaaagctgcc ctgcaccaag
       61 tgataaaaac agccaaacaa aaaatagctc ctggtccatt gagcttatta ctgaggagtt
      121 agcaggagaa cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca acccttttgt
      181 ttcgaaatga agacaaggag aaaaccaatg aaattcatga cactcaacac gcatagctgg
      241 cttgagccag aaccagagaa aaaattgcaa gaattagcag ataagatcct gttagaagat
      301 tatgaagtga ttgctttaca agaaatcaac caattactgg aatctgaaga agtagaacct
      361 gcgaagctaa tgaagttctg tccggtaaag aatcaagtac ctatccgtaa agataatttt
      421 gcctaccgtt tagttcagct tttaaaggaa cacggaaaag agtatttttg gagttgggaa
      481 atgagccaca tcggttatga taaatatgaa gaaggaaatg cactgcttac gaaaaaaaca
      541 ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga aagaagatta tcagacacgc
      601 aagatcttga ttgggaaaac aaagatagat gatcaggaca tttttgtctc gagctgccac
      661 ttttcttggt ggacagataa aaaaagtggc ttttattttg agtggaaaaa cttggaaaac
      721 tactttttgg aaactcgcgt accacttttt ttcctaggtg atttcaataa tccagtagac
      781 tctcaaggtt attatactgt aagagaaagt tgccttcttc ttcaagattc gtatgttgtc
      841 gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa tagatggttg ggaacaaaat
      901 acagaaaaat tgcgtataga ttttattttc gtccctgaag gaatgcaggt taaaaaatat
      961 caacggattt ttgatgggat cgatagtcca atcatcagtg atcactacgg ggtggaaatc
     1021 gaattagatg tgaacgaata aaaaatataa catataacat aaaaaaactg attaaaataa
     1081 aactctccaa acgattatac cgtttggaga gttttttgat cggatgtatt tatccctttt
     1141 gcctaagcac gtaaaagctt gtatctatga taaataaata aacatagcaa ggtaaaccag
     1201 ccaatcaaag taagagaaag aatgaaatag aaatagattg gcgtttgata atgaagcgtg
     1261 agctcgttga ttcctttttg ctgcttcacg attggggtcc cgatatctgt tacttcataa
     1321 tcagtcaatg tttttccatt ttgctgtagc acagtccgat cgtagacgat aacagggata
     1381 tgaacagttt tgtccccagt gtttttccac ttgattgtca aaagaccgcc tggatgtgtt
     1441 tttataaaag ttgtttcgtt ttctagtatt tcttcgttat accggtcgta tttattgtct
     1501 tttgtctcct tatagattgg cagatagtca ggtgtggttt tttggaaaac gaacaagctg
     1561 gtagacatat ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc gaatagataa
     1621 gtatgtcgac tgacaaatgt ttcattttcc cacttttcca gatgctggta taagttctgg
     1681 atcgtctgtg ctgaaaaaat aagaatgcca aatatactta aaagacggaa ccacttttgt
     1741 tcagaccact ggttcagtga aagggcaagg taaagtagaa gcaatagcgt aaatggcacg
     1801 aaaaatcgaa atggaaactg gatcaaacta ataaaaggca catttttttc gatcaatgtc
     1861 gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta caacacgatt
     1921 ttttggaata aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc
     1981 aatataggaa aggccttagg gtaatggagc caataactgc catccagatc tatcgccttt
     2041 tgactcattt ctctgttaac aaagggagga agaatcttat tcccactgta aacaatagct
     2101 aatcctgccc aaatatttgc tgtcagaaca aaataaagca agaccgactt aatcagttga
     2161 agcagtgctt ttcctttttc agtctggtta aaaaagtaat aaagataaaa agggatatac
     2221 atcaaagcta acatgatcga agacagtaaa tggatctgtg tcataagtcc cactgagaca
     2281 gcgactttga taacagggaa ctttttatga aggacgaaat cagcaatagg gatcatacat
     2341 aaaggataaa aacaagtccc ccagctagtg aatccttggt tgatagacca gtattgaata
     2401 gaatacgtac tcatgaataa aacggctact ggtacactta cccaatttcg aacattgaca
     2461 tatctcagta aaatcagcat agaaaatcct gaaataagat aaagaatgaa atttgaaaga
     2521 atctgataat gaaaccagct acctgcaagc aacacgatca atccttgtaa ataagcgaaa
     2581 aatgggccgt aaacggcatt cacgatcctg ccactttgct ggaatccata aagggaagga
     2641 aaataatgga aatccccata tttaatttgt tgcgcggcat catagaaccg attgtaatgg
     2701 aatggggcat cagcacccaa aattagttta tgtgtgaata aatgaggcat gagaaatgcc
     2761 aaagcaatga aaaaaattgc taaaaatggg ccatattttt tcaattgtcg aactcctttg
     2821 caaactttag ataaattcat catatatcct atgcgagagg taggcaagaa attagaaaat
     2881 tcatgaaaat atttatgaat tttacaagaa aaacataagt gaaagttcta aggagagagg
     2941 atgcgacata agttttgttg catctccttg ttttgaaaca gaatttttct taatactcga
     3001 aacaaagtag ctttttcatg acctctcggt aaacggtgtt caatcagctg cttctcggta
     3061 ataagctcac tagcctagaa aatatgagaa gctattttct gactagcgct cttattactc
     3121 aaagcataac gca
//
LOCUS       DACCSK010000186         3099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCSK010000186 DACCSK010000000
VERSION     DACCSK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.192"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(362..724)
                     /locus_tag="IVP39_002717"
     CDS             complement(362..724)
                     /locus_tag="IVP39_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587759.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(798..1178)
                     /locus_tag="IVP39_002718"
     CDS             complement(798..1178)
                     /locus_tag="IVP39_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7587760.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1260..2435
                     /locus_tag="IVP39_002719"
     CDS             1260..2435
                     /locus_tag="IVP39_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7587761.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2512..3096)
                     /locus_tag="IVP39_002720"
     CDS             complement(2512..3096)
                     /locus_tag="IVP39_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7587762.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          964 a          592 c          509 g         1034 t
ORIGIN      
        1 aatcaagctt cgcaagtggt tcttttttaa cgactcagtt atcttatcat gtcgttagta
       61 acatgtcaat aactattttt taaatttttt agaaatgtga tataacatcc acttcagaat
      121 caacgctgtt aaatttatca tgtatttctt ccttggtcaa ctataaatta agcattactt
      181 tgtgaaatga tttttcgatc aattattatt attcgttata caaccgtttt caacatattt
      241 tgaaaataaa atgcactaaa aaactatctt tagcagaaca tttcattgta ttttttggac
      301 caaacaataa aaaaagaagt ttgaaaaaaa ttcaaacttc tttgttcgct taatctgtat
      361 tttagaagaa aggcactccc tctacttcgt aagttccaag aacagagcgc atttgacctg
      421 gttcttccca aatatatcct tcgcctaatt cttctctttg attcttttgt tcagtttcaa
      481 agacaaacaa cggaacattt tcctctttaa tatgtgtatt tgtcaattcc atcaaactaa
      541 tcttgctaac atcgatatga actatttctt ttaagaaatt aagtatattc gctaaaccag
      601 tctttccttc agaaagttcc gctaacgcaa attccaactt gtcatcaatt gaatggacca
      661 aaaatttttt agtaccatcc tccaaatgca acataattgt accagcaact cttttgcttg
      721 ccaaaatttc cacctcgatc aacttgttct atggcttaca taaaaattct agcacatttt
      781 cagaaaaaat acacttttta tttcttattt tctttccgtt ctagttcaga taatgcttct
      841 tctatccaaa taggtaactg ctccgcaata gtggtaaatc cgatcttacg atttttattc
      901 gtgaaatacc tttttctgcg atatggaaca gctggatttg ttttttctaa cgttcttaaa
      961 gataaactga tttttttgga atattcatca atgtcgatga tctgaactgt tactggctgt
     1021 ccgactttta acaatgaatg gatatttttc gtataaccgg attgtacttc tgaaacatga
     1081 ataagtcctt gcgtctcgtt atcaagagaa acgaatgcgc cataaggctg tatccctgtc
     1141 acttttcctt caattatttg tccaatttta taagtcatct acttccctct cttcgcattt
     1201 atcattatac tgtaaatagc tcaaaaaaag aagcgacgaa aatgactgga ggtcttaaga
     1261 tgatcgaatt tgataaacag atttcaagaa aagatacaga aagtgtaaaa tgggacgcaa
     1321 ttgctgagac atatcaaatg gatgacttac ttcctttgtg ggtagcagat atggacttct
     1381 tgtctcccgg tggggtagct aaagcgttta gtgaatacat caaacatgga atctttggtt
     1441 atgccactct gtctgataaa ctatatcagg ctatcattca ttgggaacgc caacaccatg
     1501 ctgtggagtt gacgaaagaa aatatcgttt ttacaagtgg tgtgttaacc agtttagctg
     1561 cagcagtaca aacttttaca aaaccaggcg attctatttt gattcatgac ccagtttatc
     1621 ctccattttc ctctattata gaaacaaatc aacgtcacat cgttcgaagc tctttgctag
     1681 aaaaaaataa tcatttcatc atggacttgt ctgacatgga gaaaaaaatc gaagaaaaca
     1741 atgtaaaagc aatgatttta tgcaatcctc acaatcctgg agggcgtgtt tggagcaaag
     1801 aagaattgaa acagttaagc gaattgtgtt taaaacatca agtgattctg tttagtgacg
     1861 agatccatca agatttaact ttattcgatc atacatttac ttccatgcta acgattgatc
     1921 ctaatttaga tgatctacta gttgctttta cttccgcaac caagactttc aatctagctg
     1981 caatcaaaaa ttcaatggta tttatcaaaa atccagagat gaaagctgat tttgaaaaac
     2041 atttagtgat gaaccaacag cacgaaatca acacttttgg actgattgga acccaagcgg
     2101 cttatgagac gggtggagaa tggttaagcc aactgattcc ttatctagaa aaaaatgttc
     2161 aaacagttaa tcagtttttt gaacagtatc ttccgaaagt gagaataatg aaccctgaag
     2221 gaacttactt aatgtggtta gattttaccg cctatatttc agacgaccaa gaattggaga
     2281 acactcttgt tcggaaagga aaagtcgtat taaatcctgg catcacgttt ggtccttccg
     2341 gtcatgggca tatgcgcttg aatattgctt gtccagaaga aactttactg gaaggactta
     2401 atcgaatcaa gtatgcttta gagacaatcg attaaatcat ttattcaaaa taaaaggacg
     2461 agaccttact aggtttcgtc ctttttcata attactccgt accataccaa actattctga
     2521 aatttcaatt gtttcaatca ctacatcatg tacaggtcgg tcttgtggac cacgttgtac
     2581 atttgcgatt tcatctactg tgtccatccc ttctagtaca tgtccaaaga ctgtatgtcg
     2641 gaaatctagc caaggagttc cgccaccttt atacgcttca atgatttctg atgggaatcc
     2701 agcaccttct agttgtccaa gcatattagc tggcacattt gtgttattta caataaagaa
     2761 ttggcttccg tttgtgtttg gacctgaatt tgccatagat aaagctccgc gaaggttgaa
     2821 cagttcacga ctgaattcat cttcaaaact ttcgccataa atactttcgc cacccattcc
     2881 tgttccagtt ggatcgccgc cttggatcat gaaatcagga ataacgcggt gaaaaatcac
     2941 gccatcataa taaccttttt tggaaagttc aatgaaattt tgaaccgtct tcggtgctag
     3001 ttcagggaat aattgaacag taatgtcacc acggtttgtt ttgataacag cttttggtcc
     3061 tttttcactt tttaaatcta gttgtgggaa tgccatatt
//
LOCUS       DACCSK010000187         2947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCSK010000187 DACCSK010000000
VERSION     DACCSK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.195"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(449..865)
                     /locus_tag="IVP39_002721"
     CDS             complement(449..865)
                     /locus_tag="IVP39_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587763.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="IVP39_002722"
     CDS             complement(867..1148)
                     /locus_tag="IVP39_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587764.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="IVP39_002723"
     CDS             1948..2922
                     /locus_tag="IVP39_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7587765.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DACCSK010000188         2944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCSK010000188 DACCSK010000000
VERSION     DACCSK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.196"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            6..977
                     /locus_tag="IVP39_002724"
     CDS             6..977
                     /locus_tag="IVP39_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7587766.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="IVP39_002725"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="IVP39_002725"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7587767.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="IVP39_002726"
     CDS             2004..2612
                     /locus_tag="IVP39_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7587768.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          780 a          624 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctc
//
LOCUS       DACCSK010000189         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCSK010000189 DACCSK010000000
VERSION     DACCSK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.198"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IVP39_002727"
     CDS             562..843
                     /locus_tag="IVP39_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587769.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IVP39_002728"
     CDS             845..1261
                     /locus_tag="IVP39_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587770.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IVP39_002729"
     CDS             complement(1422..1688)
                     /locus_tag="IVP39_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587771.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IVP39_002730"
     CDS             complement(1646..2620)
                     /locus_tag="IVP39_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7587772.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACCSK010000190         2775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCSK010000190 DACCSK010000000
VERSION     DACCSK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.199"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(87..1124)
                     /locus_tag="IVP39_002731"
     CDS             complement(87..1124)
                     /locus_tag="IVP39_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7587773.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1571..2554)
                     /locus_tag="IVP39_002732"
     CDS             complement(1571..2554)
                     /locus_tag="IVP39_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7587774.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSSAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT          769 a          620 c          497 g          889 t
ORIGIN      
        1 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
       61 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
      121 cctaacatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
      181 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
      241 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
      301 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
      361 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgctt gatttgatca
      421 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
      481 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
      541 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
      601 agttgattca aaatcgaacc gacgcccccg gatccgttga tcactaagat ttttttcccg
      661 cgattggcat tttcttctgg agttagacca aatttttcaa ataacaattc ataagcggtc
      721 aatgaggtca acggcaaggc tgcagcttct tcatctgaaa gattttttgg tgcaagagcg
      781 acaatacgtt cgtcaactag ttggtattct tgattgcttc ctgctcttgt tgttgtacct
      841 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt cgtcacctac agcaacaact
      901 ttcccgactc catcaaaacc taagatacgt aaagcatttc ttatcccgtt atcttggcga
      961 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
     1021 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcggcaac
     1081 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
     1141 cttcatcaag ttggagcatc catacagtgt tcatttcaat atatatagta tgctatttgt
     1201 tccacctttt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
     1261 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttataaac ggtgttctcc
     1321 taccaactct tcggtcacaa gtcaaaatat tcatcaaaca tgagaagctg ttttttgaaa
     1381 ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctgatata aacggtgttc
     1441 aatcaactga ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc tatttttgtt
     1501 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
     1561 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaaa gtgtctaaga
     1621 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
     1681 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
     1741 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
     1801 tttctgtatg ttccctcgga tctttttcaa atgcataagg attcttgaaa atccctcggc
     1861 caatcatgat gccatcaacg ccatatttct cggccaattc ttctcctgtc tggcgatcgg
     1921 ggatatctcc attgatcgtg attagtgttt gtggagcgat ttggtcacgt aaagcaatga
     1981 ttttaggaat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
     2041 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
     2101 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagctg
     2161 cttcaatcaa ttgtgccgct acttctggac gaaggatcaa gccactgcct tttccacgtt
     2221 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
     2281 ccatagcact gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
     2341 tcggctgttc atcttctgta aatgttaatc ttcctcgtac actgtctttt ccatctggat
     2401 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
     2461 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
     2521 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
     2581 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
     2641 gcaaagtgaa cagctcagaa ggtttgtctg attgaacaaa aagaggactg tgacaaaaat
     2701 cttcacagtc ccttttccgg aataaatggt gttctcctgc caactcctcg gtcacaagtc
     2761 aaaatattca tcaaa
//
LOCUS       DACCSK010000191         2748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCSK010000191 DACCSK010000000
VERSION     DACCSK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.200"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            5..979
                     /locus_tag="IVP39_002733"
     CDS             5..979
                     /locus_tag="IVP39_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7587775.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAIIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            complement(2286..2651)
                     /locus_tag="IVP39_002734"
     CDS             complement(2286..2651)
                     /locus_tag="IVP39_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587776.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          998 a          349 c          449 g          952 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagtcac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaattatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaagcta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgatat
      901 gaataaagaa gacttagata gtcataagtt aattagttat ccaattatag aaaaacaaca
      961 aattaaatat ataaattagt taatgtgttg tgattgatta tttaatatag atataataca
     1021 aaaaggcaaa gatattttca aagcattttt tcttgatgtg atgtccttgc cttttttgta
     1081 atgtttttaa cttattgcct ttgcaaaatt tatttacctg ttttctggat caacgatagt
     1141 gtatattatg ctatccttgt ttggatttta ggacgtttaa agaggcactt ttttacataa
     1201 cttaatccat agacctttta agcatgctgt gagataataa taaagaagag tttaaagcgc
     1261 accccaaacc acttccccat aagtgtatta cgctttaaac tcttttatat ttgaattcac
     1321 taagaagcat accatatatt tgaatttact aagaagcata ccatatattt gaattcacta
     1381 agaagcatac catatttttg aaaaaaagtg agaaaaaagg cttataatta ggatttagag
     1441 taattaatta gatgtttatt ttataggact gatactatag attacaaata gtatcgtatt
     1501 tcgcaatctt aaaattatct tcttactatg aataagataa taaaatttgt atttgtcctt
     1561 tttaggtaac ttatttaatc tatatacctt tctggatcaa cgaaagtata ctttatatag
     1621 tcataacgcc gatggtaact atgcactgta acgataatgc tgctaaaata tctttttacg
     1681 aaaaacatca caaggaaaaa cttaactatg gtattatcta aacataattt tttcggtatt
     1741 taatatttaa ttaggaggaa atattttgct tttttatagt aattttatct taatagtagc
     1801 gatattatta cttctgaata tatggatttt tgataggagt agaaatgcaa gtataggttt
     1861 tagaactaaa cgaagtctgt cttctaaaaa aattgggtat actcacaaac tattttttat
     1921 ggaggaattg ttttaattag tttactttct tcaacacttt attctttgaa tattatagat
     1981 gtttccacct caaattcaat atctattatt ggaatcatta ttgctgcaat tattacacaa
     2041 ctgtttttag tgtttggaga gaagaaaaga agtaaaaaat agaaaaacta gtgatagttt
     2101 tggtgatagt gatattaaaa atcagaaaag atagaaaaag caggagatag ataaaatgct
     2161 gatatttcag cattttttaa gagtataaaa gagaaaaaaa gtaataaatg tatacatgat
     2221 ataagaaaaa catatatgta taaaaagaag aaagtagctt ctctttgtac atatatggtt
     2281 tgtttttatg gatgccgagc ttttttataa gataaggcta ccgcccataa cgacccaagg
     2341 atttgtggaa ctaagtaaat acctgcaaga agcaagatca gctgggtcgg ttttaaagga
     2401 tgatagaagt aacgccattc agtaaaattg gcgcaaaata aataggaagt gacagtagat
     2461 ataatattgg caatgaatag agatataata cgcctgttcc tttgaaaata atatccagca
     2521 aaaagagata aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg aaaatcaagc
     2581 agtaaggcga aatgcacata aggaacaaaa gataaaagca acggaaggtt tttccagcaa
     2641 gtttttatca tctttcatca ccacctaaca agtgttatta ttttgttagt tttattgtaa
     2701 attgaatcct gcaataatca attacttttt cattatttct agattaaa
//
LOCUS       DACCSK010000192         2732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCSK010000192 DACCSK010000000
VERSION     DACCSK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.201"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..335
                     /locus_tag="IVP39_002735"
     CDS             <1..335
                     /locus_tag="IVP39_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7587777.1"
                     /translation="KLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKK
                     TGGLRYAPALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKRED
                     FNEAQKIR"
     gene            351..1112
                     /locus_tag="IVP39_002736"
     CDS             351..1112
                     /locus_tag="IVP39_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7587778.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            1243..2577
                     /locus_tag="IVP39_002737"
     CDS             1243..2577
                     /locus_tag="IVP39_002737"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7587779.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT          926 a          464 c          585 g          757 t
ORIGIN      
        1 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
       61 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
      121 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
      181 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
      241 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
      301 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
      361 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
      421 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
      481 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
      541 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
      601 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
      661 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
      721 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
      781 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
      841 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
      901 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
      961 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
     1021 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
     1081 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
     1141 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
     1201 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
     1261 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
     1321 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
     1381 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
     1441 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
     1501 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
     1561 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
     1621 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
     1681 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
     1741 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
     1801 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
     1861 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
     1921 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
     1981 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
     2041 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
     2101 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
     2161 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
     2221 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
     2281 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
     2341 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
     2401 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
     2461 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
     2521 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
     2581 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
     2641 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
     2701 caatcaaatc aaaaacatga gaagctgttt tt
//
LOCUS       DACCSK010000193         2699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCSK010000193 DACCSK010000000
VERSION     DACCSK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.202"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            13..2190
                     /locus_tag="IVP39_002738"
     CDS             13..2190
                     /locus_tag="IVP39_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7587780.1"
                     /translation="MKLVSQYMGPEHVAFVEKACEYATAAHDGQFRKSGEPYIIHPIQ
                     VAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLVDGVTKLGKIKYK
                     SHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQRRIAQETLEIYA
                     PLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYVSGTVEDIRIATE
                     ELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIKDCYAVLGAIHTK
                     WKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHEIAEFGVAAHWAY
                     KEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFSDKVYVFTPKGDV
                     TELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDIIEIMTSPNSFGPS
                     RDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFTPKDILTKNKLQE
                     ALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQEAEEIMNQPKKE
                     PDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGISIHRRDCPNVQP
                     DKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTVNALTKNLNSVEA
                     RTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            2201..2647
                     /locus_tag="IVP39_002739"
     CDS             2201..2647
                     /locus_tag="IVP39_002739"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7587781.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRIRGITVATGRFGADMSVSLVNDGPVTIIIDSKNK"
BASE COUNT          958 a          487 c          599 g          655 t
ORIGIN      
        1 ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt
       61 gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa
      121 ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc
      181 catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa
      241 gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta
      301 gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg
      361 ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac
      421 atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta
      481 gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa
      541 gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag
      601 acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagca
      661 acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc
      721 tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt
      781 cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa
      841 tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac
      901 caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga
      961 acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa
     1021 ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag
     1081 atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga
     1141 gatatcttta gtgataaagt ctacgtcttc acaccaaagg gagacgttac tgagttacca
     1201 aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg taacaaaacc
     1261 actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggcgat
     1321 atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta
     1381 gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa
     1441 gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg
     1501 cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa
     1561 acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc
     1621 aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca
     1681 gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt
     1741 ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg
     1801 attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt
     1861 cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc
     1921 gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc
     1981 tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat
     2041 ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt
     2101 ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat
     2161 gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg
     2221 ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct
     2281 acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa
     2341 aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga
     2401 agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa
     2461 aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga
     2521 atcattcaat gaaggaatca gaattcgcgg catcacagtc gctaccggac gattcggagc
     2581 agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa
     2641 caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttaga
//
LOCUS       DACCSK010000194         2633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCSK010000194 DACCSK010000000
VERSION     DACCSK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.203"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..182)
                     /locus_tag="IVP39_002740"
     CDS             complement(<1..182)
                     /locus_tag="IVP39_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587782.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKE"
     gene            complement(279..944)
                     /locus_tag="IVP39_002741"
                     /pseudo
     CDS             complement(279..944)
                     /locus_tag="IVP39_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1062 family transposase"
     gene            complement(1026..2480)
                     /locus_tag="IVP39_002742"
                     /pseudo
     CDS             complement(1026..2480)
                     /locus_tag="IVP39_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
BASE COUNT          836 a          530 c          415 g          852 t
ORIGIN      
        1 ttttctttat actggtgcca ataatcaaga gcagtcttgc cttgcttgaa cttattgact
       61 acattataaa tagtttgtat agcacgtccc attcggttag cgatttcaac cggcttatta
      121 tgttgaagat aatatgattc tatcattgtc agttcgtcta tggtaagatg tttgtaggtc
      181 atttatggtt acactccttt gttttctttc gtcggaaata caatttgagt gtaccataaa
      241 tgccttttta tttttctaac ttaattttac aattagcgaa aagtcattga tttgaataga
      301 tgcttaggta ctgattgcat ccattggttc aaacgatttt caatatctat agctcgtctg
      361 ccttctggct gaagagtgat gattacttta gataatcgct caacgagggt aataacagca
      421 cttttgtgct ttttacccac aatagtatcc ccttcaaggt ggccaaattc ttgattgaat
      481 ttagaataat catttcctct gtcacgaagt gatcgacgaa aagattgttt tccccgagtt
      541 tctttgtgtc cgttggcttt acgttttcct ttcataggta ggtgagtcac ttcaaatacg
      601 ccttgtttaa acatacgata aagtgtacgc atagaacaag aaatagaaaa ctctgcacgg
      661 ccaacaatca catcaggtgt ccaaccttga acaaccctct tttgaatata ttctgtttgt
      721 ttatctgata agacaatagg acgtctgcca cagcgagttt tattcttctt gtattgattg
      781 aaataatcta gagcattgtg ccctgttttg aaaaattggt aaactttatg aatcgtttgt
      841 ctagatctat tcaatagtac tgcggttttg gctacagatt gatgattatt ataatacgcc
      901 tctatcattg caagtttatt tgatgtaaga tgggtatagg tcatttgtct tcactcctta
      961 taattctttt gcgggaacta tcttgagtgt agcataaatg acttttttat ttgtctagct
     1021 taattttaca atcggcgatt gtcacttgca atatttactg gaatttcgct cgtatcttct
     1081 tttacttcct cagtctcttc atcaatacct aaaatgaatg aagcaataaa accgacaaca
     1141 aatgcaatcg cacaaccaat cgctgcgtaa ataaaatttg atagtccttt ttccccaaag
     1201 aaacttggta acgcaagaat gccaggagca acttgagcat atcgaccgac acccatgatt
     1261 ccaataaata gtcccgctgc tccaccacct accattgaag caattaacgg ctttttgaat
     1321 cgcatattaa ttccgtataa agctggttca gtaattccca taaaagcaga cagacctgaa
     1381 gaaacagcta attgtttgac agaaagttgt tttgttctaa aggcaactgc taaggcagca
     1441 ccaccctgag caatattgga aaccatcata cctggaccag caacagtatc aaatccagat
     1501 gttactaaca tattaatacc gattggaatt aaaccgtaat gcatacctgt catcacaagt
     1561 aatggagtaa atgcacccac aagtaatgga accaaccaac ttgcatacgt attaacaaat
     1621 tcaataagtg accctaaccc attacctaag aatgtaccca aaggaccaat cacaattaag
     1681 gtaattggtg caacaatcaa caatgttaac agaggcgtca taaatattcg aacagcattg
     1741 ggcatcaatt tatcgataat cggctcgata tatgacataa atcaaatagc taaaataatc
     1801 ggaataacag aagaaccata gttagctaac gtcacaggta atccgaataa agctaaggac
     1861 tcacctgttt ctctagcacc cgcaatcatt gctgtaaaag tcggatgtaa caaaattgct
     1921 cctaaggtaa ttgcaatgta ctgattacat ccgaattttt ttgcagcaga actagctaat
     1981 aagaccggta aaaaataaaa ggctgcatca ccaatgaaat ttaaaaattg ataggtttgc
     2041 gattctggtg tcatccattc caacatgact aatagagcta ataatgcttt taacatccct
     2101 gcacctgtta atgccggaac aattggaaca aaaatacccg caattgtatc taaaactttt
     2161 gatacaatcc ctttattgtt tgcttctgaa ctactttctt tagaagcgga aaaatctcct
     2221 agtttatcga tttcggcaaa tacaaatttt acatctccac caataatgac ttgaaattga
     2281 ccaccctgat taactacacc tacaacacct ttaagatttc taatagctga ttcatctgct
     2341 ttactgtcat cttttaaatt aaatcgaagt cgtgttgcac aatgcgctaa actgcttaca
     2401 ttttcagaac caccaacatt tgctaatatg tcttttccta attctttata ccccatgcta
     2461 ctttcctcct tagagaacat tttctctttt gataatttga atatagcgtg ctattaatag
     2521 ataaacaata catttgaaac cgttttacac agcgttgcga aattcattca gtttacgatt
     2581 attttttcgc aacactgttc caaaaaaata aaatccactt actaactaat cgt
//
LOCUS       DACCSK010000195         2582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCSK010000195 DACCSK010000000
VERSION     DACCSK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.204"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IVP39_002743"
     CDS             <1..704
                     /locus_tag="IVP39_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7587783.1"
                     /translation="EETQGNVIVIDNDTFKQQHPNFDELAKLYEKDVVKHVTPYSNRM
                     TEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTI
                     ERYETMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSS
                     LETPSISPKETLERELNRKVSGKEIQPTLERIEQKMLQNQHQETPEFKAIQQKMESLQ
                     PPTPPIPKTPKLPGL"
     gene            1145..1462
                     /locus_tag="IVP39_002744"
     CDS             1145..1462
                     /locus_tag="IVP39_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587784.1"
                     /translation="MEKTKKLQLEDFTENEFYGTQEQQYLKAQVREELKEQGFIIDSS
                     FEGDFKTWIGVYARPKDKPTYLDPQNDKEAEEQEQYSINGFKQDFSEWFEWEIKNLKI
                     KEM"
     gene            1996..2493
                     /locus_tag="IVP39_002745"
     CDS             1996..2493
                     /locus_tag="IVP39_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7587785.1"
                     /translation="MKYIKNVETLEELKKAYKKLALKLHPDCGGNEEEMKILNNEYDE
                     LFSKLKNTHKNKEGETYTKETTETPEQFKDIINKLFNLKMDGVAIEVVGTFIWLTGNT
                     KPYKDDIKALEFRYSPKKYAWYKAPSDYKKRSRKNYDMDTIRGMYGSQKIKEEKEEKK
                     YLQAN"
BASE COUNT         1064 a          446 c          441 g          631 t
ORIGIN      
        1 ttgaagaaac acaagggaat gttattgtca ttgataatga tacctttaaa caacagcacc
       61 ctaattttga tgaactagcg aaactttatg agaaagacgt agtaaaacac gtcacccctt
      121 attctaatcg catgacagaa gcgatcataa gccgtttgag cgatcaaggc tataatttag
      181 tgattgaagg tacaggacga acaacagacg ttcctattca aaccgcaaca atgcttcaag
      241 ccaaaggtta tgaaacaaaa atgtatgtta tggcagtacc taaaatcaac tcatatttag
      301 gaacaattga acgatatgaa actatgtatg cagatgatcc aatgacagcc agggcaacac
      361 caaaacaagc gcatgatatt gttgtcaaaa acttaccgac caatttagaa acccttcata
      421 aaacgggctt atttagcgat ataaggcttt acaatagaga aggagtgaaa ctctattcaa
      481 gcttagaaac gccttctatt agtccgaaag agaccttaga aagagaatta aatcgtaaag
      541 tatcagggaa agaaattcaa ccgactttgg agagaataga gcaaaaaatg cttcaaaatc
      601 aacaccaaga aacccctgaa tttaaagcaa ttcaacaaaa aatggaaagc ttgcagccac
      661 ctacaccacc aatacccaaa acacctaaac ttccaggact ttaaacctta aaaaaggaaa
      721 agagtagtta ccaaaaaacg gtaactactc tttttttata aaaacatttc ctcatgtttt
      781 aaaagctgat cgagaaataa agcacacaac ggtattcttt cttttttcag ttggtacatt
      841 ttgcagtctg taacgtgtca tttactgttt gttaccttaa catatccagt cgaaaacaca
      901 agggtatata aacgagcaag ctcgcccttg tgttttcttt gtgtctatgt tgcgttctcg
      961 tcacagcaaa cgacacgaac acactggcga aaatgaacca acaaaacgaa aaaagaaagg
     1021 agcgtacact gtaatttatt tctagctttt aaaacatgag gaaaacagct tcacttcctc
     1081 aaacgcctaa caaaaatggt tgtcaaaaaa ctaaaaatca aaaaaaagaa aagaggaaaa
     1141 gaaaatggaa aaaacaaaaa aattacaatt agaagatttt acagaaaatg agttttatgg
     1201 aacacaagaa cagcaatatc taaaagctca agtaagagaa gagctaaaag agcaaggctt
     1261 tatcatagat agcagttttg agggcgattt taaaacatgg ataggtgtat acgcaagacc
     1321 aaaagataaa ccgacctact tagaccctca aaacgataaa gaagcagaag aacaagagca
     1381 atattctatt aacggcttta aacaagattt tagcgaatgg tttgagtggg aaataaaaaa
     1441 tttgaaaatt aaagaaatgt aaaagaaaag cctagcaaaa aaagctaggt ttttctttta
     1501 cataaaacac caaaaagaaa aacctaagaa ggataaaata agttgttcca cttgcggtgc
     1561 agttgctcct cgttccaaac ccaacttatt tctttgcaaa gatatttata tttgactaac
     1621 acctttcctc gaatggtaaa agctaaccgt tcgttactct acctcaaata ctttcttttt
     1681 tcagttggta cattttgcag tctgtaacgt gtcatttact gtttgttacc ttaacatatc
     1741 cagtcgaaaa cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttgtgtct
     1801 atgttgcgtt ctcgccacag caaacgaaac gaacacactg gcgaaaatga accacctaaa
     1861 cgaaaaaaga aaggagcaag atatgtagaa cactcacagc ttttaccatt cgaggaaaac
     1921 agcttcactt cctcaaacgc ctaacaaaaa tggttgtcaa aaagccaaaa atcaaaaaga
     1981 aaaggaatga taaaaatgaa atacattaaa aacgttgaaa cattggaaga attgaaaaaa
     2041 gcctacaaaa agttagcttt gaagttacac cccgattgtg gcgggaatga agaagaaatg
     2101 aaaattttga acaatgaata tgacgaactg tttagcaaac ttaaaaacac tcataagaat
     2161 aaagaaggtg aaacttacac caaagaaaca acagaaacac ccgaacagtt taaggatatt
     2221 attaataaat tgttcaatct aaaaatggac ggcgtagcaa tcgaagtagt aggaacattt
     2281 atatggctaa caggtaatac aaaaccttat aaggacgaca taaaagccct agaatttcgt
     2341 tattcaccaa aaaaatacgc atggtataaa gcaccaagcg attataaaaa acgtagtcga
     2401 aaaaattatg atatggacac aataagggga atgtatggaa gccaaaaaat taaagaagaa
     2461 aaagaagaaa aaaaatactt acaagccaat taaggcttgt aagtttatca gaaaggaaga
     2521 aaccattatg ttttataaaa taagaataat tgaaactttg gaaaaggaat ttgaaattga
     2581 ag
//
LOCUS       DACCSK010000196         2485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCSK010000196 DACCSK010000000
VERSION     DACCSK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.205"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1015..1305)
                     /locus_tag="IVP39_002746"
     CDS             complement(1015..1305)
                     /locus_tag="IVP39_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587786.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2205)
                     /locus_tag="IVP39_002747"
     CDS             complement(1513..2205)
                     /locus_tag="IVP39_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7587787.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          734 a          459 c          349 g          943 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgagttt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2341 ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat
     2401 ttatggtgaa caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag
     2461 attactgtat ttattacaaa aaacg
//
LOCUS       DACCSK010000197         2403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCSK010000197 DACCSK010000000
VERSION     DACCSK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.206"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IVP39_002748"
     CDS             complement(<1..132)
                     /locus_tag="IVP39_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7587788.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     "
     gene            complement(206..487)
                     /locus_tag="IVP39_002749"
                     /pseudo
     CDS             complement(206..487)
                     /locus_tag="IVP39_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            949..2331
                     /locus_tag="IVP39_002750"
                     /pseudo
     CDS             949..2331
                     /locus_tag="IVP39_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          398 g          718 t
ORIGIN      
        1 atgacaaaca tttattccac gatcatataa caattcctga acttcacgat agcttagatt
       61 gtaacgcagg tagtaaccaa cagcgacaat aatgacgtct tttttgaatt gtttgccttt
      121 aaaatgattc attactctgc cctctctgtc ttttttctca attttacact aaaatagatt
      181 ttttggaaaa ctttgcaaca gaaccgaaat gagtatagta tgaattagta atttggtttt
      241 catctaagtt tagctttgat ttactaaaat tgatatctcg atacatgttc aatacatcta
      301 atacaaatcg agaatcttct ttcggaaatg gctcagaaag atcactccaa atttcatcat
      361 cataatgata ctcgtaacct tctctcaata tttcgatttt tctttcataa taagatctgt
      421 tttggtcctc aaatttagcc aataactcaa attgatttac tagtgacaat ctctgaaaac
      481 tgtccattaa atgtttcttc ctatcttgtt cattaacaaa aaattataag cccttacaac
      541 gaatattata tattaaattt aattgtaggt gaacacgaat tttataaata tttatggtgt
      601 taatgacaaa tgatatccga ctcctcttca tacgttctgg actatttgct aatagattaa
      661 aatttcagca tctatttctg accataaata aatgagctgc cgaaaagctg ccaaatcaga
      721 cttgaaaata aaggatcaaa aacttttaag tttctgaccc cgtaactcac acttcaaaat
      781 cttacttaaa aaggagtctg aaagaaaaaa agagtattca aaattttttt ctaactagct
      841 acatcacatc tatgcattga cactcaaaga accagaataa gtcatcagaa agcaaggggg
      901 tgcggacata atcctggacg aaaaaataaa cttaggagga atgtccgtgt gttttcccac
      961 aaagagcgtg taagggcaat tcagttattt ttaaaatacg attgctctta tgctgcaacc
     1021 attcgagaat tagggtatcc gtcgattggt gcactacgga aatggtataa agagtatttg
     1081 gaatccggag agctccatca ggagcaccga aaaaaatcaa agtattctga agaacaaaaa
     1141 cgaatagcgg tgaatcatta ttttgaatat ggtcaatgtt atgcacgaac cattcgcatg
     1201 cttggttatc ctaatagaga gtccttgcgg cagcggtgtg aagaacttgc accaggagct
     1261 agaaaactcc gaaaaagtgc ggtaaagtta acacaagatc aaaaagaagc tgtgttgaaa
     1321 aaattctata aaccgcaaac taataggaaa aatttagccg aatctggagg aattagtaga
     1381 gttactctat atcaatggaa aaacacggtt cttggaaagg attttccatt gtgtatgact
     1441 ttaaaaaatc aggaaaaaca aaaagagcta cttttaaagg aagtagaaga cttacaaaaa
     1501 caagtacacc aattacagct cgaaaaagca ttactcgaag gagcggctga actgctaaaa
     1561 aaagaaaaag gcgtcaatct tctttgtcta tcaaatcaag aaaaaacgat attgattgat
     1621 gccttacgaa atcagttcac cttgaaagag cttcttcaac agctccagtt tcctaaaagc
     1681 agctattttt atcaaaaaca agcacttgag aaaccagata agtattacaa agaacgtcaa
     1741 attaattatc acgattttca atcataactt ctgtgcgtat ggttaccgta gaattcacca
     1801 agctttaaaa aatatgggta aaaagctttc tgaaaaagtt gtacgtagat tgatgactga
     1861 ggaaaatcta tttgttaaat tttctagacg aaaaaagtat tcttcgtatg ctggggaaat
     1921 ttctcctgca caccccaatt tattaaatcg gaattttaaa gcaaagaagc caaatgaaaa
     1981 atggttaaca gatattactg aatttagaat tctcgcaggg aaaatctact tatcaccact
     2041 tgtcgattgt tacgatggtg ctattgttag ttggactatt ggaacaaagc caacttgctt
     2101 cactctatgt ctaaaaagag ttattcacca gataattcag cgtgcgaagg tttctttgga
     2161 cgattaaaaa acgagatgtt ttatcaacgt gactggaaga atacaacaat caatcagttt
     2221 ataaacaaat tggatgatta tattcattgg tataataatc aacgaattaa gttatcttta
     2281 ggaaggctaa gccctcttca atatcggaag aagcaaagat atatttgtta aaaaggtcca
     2341 aaaaaaatat ccgcaccccc ttttttttgt aaaaaactat tgcacaatac aagtagccta
     2401 cgg
//
LOCUS       DACCSK010000198         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCSK010000198 DACCSK010000000
VERSION     DACCSK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.207"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            208..2070
                     /locus_tag="IVP39_002751"
     CDS             208..2070
                     /locus_tag="IVP39_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7587789.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2400
                     /locus_tag="IVP39_002752"
     CDS             2070..>2400
                     /locus_tag="IVP39_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7587790.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
//
LOCUS       DACCSK010000199         2392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCSK010000199 DACCSK010000000
VERSION     DACCSK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.208"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..709)
                     /locus_tag="IVP39_002753"
     CDS             complement(<1..709)
                     /locus_tag="IVP39_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7587791.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
     gene            892..1743
                     /locus_tag="IVP39_002754"
     CDS             892..1743
                     /locus_tag="IVP39_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587792.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            1764..1976
                     /locus_tag="IVP39_002755"
     CDS             1764..1976
                     /locus_tag="IVP39_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587793.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT          823 a          357 c          462 g          750 t
ORIGIN      
        1 cattactgat ctgtttccaa tttgaaaatt cctttccaca atcaaaagtg atggatttga
       61 atagattttt cggtacagat tcaaaccaat gatttaattt gttttcaata tcaatcgctt
      121 gtctaccaca cggtttcaat gtgatgataa cttttgataa tcgctcaact aaggtaatta
      181 cagcactttt atgtttcaaa cctacgatag tgtcaccttc aaggtgacca aactcatttg
      241 agaattggct ataatctttt tcacgttcat gaatagagcg acggaaagct tgttttccac
      301 gtctttcttg atgtccattt ggtttacgct tgcctttcat cggtaagtca gaaggattaa
      361 atagcccctt tttgaacata cgataaatgg tacgagcaga acaacgaata ggaaacgctg
      421 cacgaccaac aatcacatcg ggtgtccatc cttgaacaac ctttctttga atatattctg
      481 attgttcctc aggtaaaaca agcgggcgtc taccacagtt tgatttgttt ttcttatact
      541 gttgatagta ctctaaggct gattttcctt gcttgaagaa caggtatact ttatggatcg
      601 tttgtcttga acgattcaag aaatgcgcag ttttcgcaac agattgattt attttgaaat
      661 aagactctat cattacaagt tcatccgttg taagatgggt ataggtcatt tgtactcact
      721 ccttataatt ctttggacgg aactattttg agtgtagcac aaatgacttt tttagttgtc
      781 tagcttaatt ttacaatcgg cgtacttaaa aaattggttc attgagcaac ctatcgctgc
      841 taaagtagca aaggaaaacg ttaaaggaaa gaatttaggg aagaaatcga aatgaaatcg
      901 gacgactgga ttgaacacac ctggcttcct ctgaaaagaa atagcaaacg aaagaaattt
      961 tgggtattgc aattatggcc attaggaaaa ctttgtagtt acggggcacg acttattctt
     1021 gatttaaccg tttactcgat tggagtattt ggaacggctt attacttgat aaatgatccc
     1081 aataggattg cagcatcaga catttttaca gtgctaatga cgttgatttt tgtcatgcta
     1141 ttgtctattt ttactcaatt agtcatgcaa tttaccaggc aaaaattaac agcaggaaaa
     1201 cttttcaaaa gcctattcga tttaattgtt gtaaatgcaa ttctagcgac tttaataaaa
     1261 caaattgaac aatctggaag aacaacaaaa ggagtgaagg attttttatt gacgtttatt
     1321 cactttgtaa atggacacat gtatttgttt gaagtgggta ttattctctt ttttgtcggg
     1381 tatgtatcca cgaaacttat taaacgttca attgaaaaag aaattgaaat tgacctgatt
     1441 ccgacacttc ttggttatga gtacaatgtt gattttggaa gatatgacga attgatcaat
     1501 acggatatcg ttcacacgag tatccatgaa tctactgtta agggcatcag atctatttta
     1561 aaaaatggcc aggaaatgaa agcaatagat cgaagaaaag agatctacaa tttttatgaa
     1621 gtcgaagcaa gcctacgata tggcatcgga aaattgtggt tagatggaag taatattcgt
     1681 caaaacaaac catacatttt ttggaacagt agtgatttag aacttccaat gaaagataaa
     1741 taattatgga gaatcgtttg aaaatgattc ctctatacag gattgtgctt atagttggca
     1801 tggagttaaa aaccttaaag aaggagcagt tattcaaagt gaaagaacgt cataatcagt
     1861 tactatgtag taaaacgggt acgatgaaca ttgaataccg aacagaatat ttagaattaa
     1921 cgaatcaaac tgattttttg ttgattcgaa agcaacaaga gcaagaaaaa aattaatggc
     1981 tacttatagc caattgattg atgaatggga acaattcaag gaatctaaaa ttccagaacg
     2041 tctctaaaag cctttatttg taaagagcat aaggaaacgt gggaacactt tttaaaaaaa
     2101 gtgttccaat aggctgtgcc tattacgttt ctcaaaaata aatgtgggaa caaaatattt
     2161 aaaaatagga tagtgaaagg atagtgaagg atagtaaaag gagggtagta ggatagcaaa
     2221 aaggataggc tccttttcgc tatccttttt aaattggagg aattaatatg gaagtaagaa
     2281 taagaaatgt ggatgagaaa atcgtaagag aaattgatcg aagatgtgac gaacggttct
     2341 gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag at
//
LOCUS       DACCSK010000200         2253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCSK010000200 DACCSK010000000
VERSION     DACCSK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.209"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IVP39_002756"
     CDS             <1..75
                     /locus_tag="IVP39_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7587794.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            251..592
                     /locus_tag="IVP39_002757"
     CDS             251..592
                     /locus_tag="IVP39_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587795.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWGHVYLIHLWKQPRKPEYIYVIEDGLIDALEEYDRDNLIDISYYGQGKK
                     YIAEMEAEFDE"
     gene            585..1331
                     /locus_tag="IVP39_002758"
     CDS             585..1331
                     /locus_tag="IVP39_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7587796.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPQDVSMLEPASQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            1337..2023
                     /locus_tag="IVP39_002759"
     CDS             1337..2023
                     /locus_tag="IVP39_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587797.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2027..>2253
                     /locus_tag="IVP39_002760"
     CDS             2027..>2253
                     /locus_tag="IVP39_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587798.1"
                     /translation="MDHRSYYAIIPANVRYDDSLIPSAKLLYGEITALCNEKGYCWAS
                     NEYFANQYGVRKSTIQNWLKSLEEKGYIYREV"
BASE COUNT          827 a          387 c          482 g          557 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaaa aataggcgta ataattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatt gataagcaaa aagcatggca
      181 acaaataaaa agcgactccg ccggcaagca ttgagtcgca aacaaaatac atctaaggag
      241 atgttaccac atgaaaaaag aactttccac tctagatcaa tatttgactg atcctagttg
      301 gggcaaatcg aatatcaagg aaacaagcaa tcgaaaaatc agacgtaatc ttttgacgga
      361 tgaagaacta gcatgtgatc aagacgattt gggaaatttt gtgagtattt ggggacatgt
      421 ctaccttatc catctatgga agcagccaag aaaacctgaa tacatctatg tcatcgaaga
      481 tggcttgatt gatgcactag aagagtacga cagagataac ttgattgata tctcttatta
      541 cggacaaggc aaaaaataca tcgctgaaat ggaggcagaa tttgatgagt gaaaaggaac
      601 aacctttgaa aaatagaagt gataatacgc tttttaatac cttatacaaa ataaatgtga
      661 aagatgttac tgaaaaacga aataacctca cttatctttc gtgggcatgg gcttgggcag
      721 aagttagcaa agtgtgtgag gcagtagatt atgaaatcta tcatgatcca gaaacatatc
      781 tgccgtatgt ctttgataag aaaacagggt acatggtttt taccagtatc acagtcaacg
      841 gagtaaagcg tgacatgtgg ttaccagtca tggatggtgc aaacaaggca atgaaagatg
      901 agccatatac ctacgaagtc aatgattatc agtggaataa cgaaacgaag aaaaaagaga
      961 ttgttggaaa aatcgaaaag cgagttgaag cagcaactat gtttgatatc aacaaaacga
     1021 tcatgcgctg tcttgtaaaa aatctagcaa tgtttgggct agggctatat atatttgctg
     1081 gcgaagatat gccacaagat gtctcgatgc ttgaaccagc tagccaaaga agcaaaaagc
     1141 tattcttaga tgctttacaa ctggttgcta acaagtacga aaaatcgatt gatgaagcaa
     1201 ttgttgcatt gactgatgca gcttccataa ccgccgatga cagtaaatgg accaagagag
     1261 acttgggcat tctaaaacga ggcgttaact ggattgaaga tcagtacaga gaagaaacaa
     1321 aagagaagtg atatgagtgt ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa
     1381 gttcaaagga aaagacatag gtgcaacgat caatgaagaa gtgaacattg atcgactaaa
     1441 gacgatgtat gacggctacg atggcgatcg agtcattgaa attcgtttta ttgatcctag
     1501 acgtttcacc gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga
     1561 tacaggcatg ccaacggact tctggaagga attcttctac ttccgttttg aaggcgtcac
     1621 agggcgcaaa ataagcctca aagacgaatc gaatacgact gtgagtgatg ctaatgtctt
     1681 agcaaatatc attttagatt tcatctttga acaccatatt cctttcaaag aaggctatga
     1741 gattttacca gcgaatcaag agtattactt ctacaaatgc atcacaaaaa gagtctgctg
     1801 catctgtggc aaaacaggag ctgatattga tcactttgac aaagctttag gaagacgaaa
     1861 gcgcaaagaa gttgatcatt cagagtacac atttgcagca ctctgcagaa tccatcacac
     1921 agagaagcac aagataggtg tgatcaattt caaaaataag tatcaaatca aagggatcaa
     1981 gttaaaccag gaaacaatca agaaattaag aatcggagga tgaaaagtgg accacagaag
     2041 ttattacgct atcatacctg caaatgttag gtatgacgat tctttgatac ctagtgcaaa
     2101 acttctttat ggagaaatca cagctctatg taatgagaaa ggttattgct gggctagcaa
     2161 tgagtacttt gccaatcagt acggtgtaag gaaatcaacg attcaaaatt ggctgaaatc
     2221 tcttgaagaa aaaggctata tctatcgaga agt
//
LOCUS       DACCSK010000201         2038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCSK010000201 DACCSK010000000
VERSION     DACCSK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.210"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..1431)
                     /locus_tag="IVP39_002761"
     CDS             complement(<1..1431)
                     /locus_tag="IVP39_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7587799.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQ"
     gene            complement(1438..1905)
                     /locus_tag="IVP39_002762"
     CDS             complement(1438..1905)
                     /locus_tag="IVP39_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7587800.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          544 a          433 c          324 g          737 t
ORIGIN      
        1 ctgttttgct tcaattgcag aaactttttg cagcttttta cgtagtgctt tttcttgtct
       61 gcgtaattga gcagcatttt caaagtcttg tttttggatc gctgcttcct tttcttgtac
      121 tagttcatcg atttctagtt tgatcacagt tgattttgtt aagtgatcgg tttgatccaa
      181 acggactttc gctgctgatt catcgatcaa atcaattgct ttatcgggta gttgacgaga
      241 agtgatataa cgaacagaaa gattgactgc tgctcgtaca gcttcatctg tgatctccac
      301 tccatggtgc tcttcatatc ttgtacgtaa tccttgaaga atcacttctg cttcttcagg
      361 agttggctca tctacttgaa tgcgagcaaa acgtcgctct aatgcagaat ctttttcgat
      421 atatttttga tattcatcta gggtcgttgc tccgatcgtt tgtaattctc ctcgagctaa
      481 cgccggtttc aagatattcg aagcatcaat tgctccttct gctccacctg caccaattaa
      541 ggtatgcaat tcatcaatga ataagattac ttgaccatca ttatagattt cgtcgatgac
      601 ctttttcatt cgatcttcaa attcaccgcg atatttcgta ccagcaacca aagatcccat
      661 atcaagcatc atcaggcgct tttcttgcat gtcttctggc acttcaccta aaatgatttt
      721 ttgagctaac ccttcagcaa tggctgtttt gcctacacca ggttcaccca ccagtacagg
      781 gttattcttt gtccgtcggc taagaatctg gataagccgt ttcacttctc tcgaacgacc
      841 aacaactggg tccagcttgt tttcttttgc taatttcgtc aaatcacgtg ctaatgaatc
      901 tagcgtaggt gttccttgcg gtgtttgccg tttatcttgt acatttacat ttctacgacg
      961 attcattcct tttccagcag aatcaccagc tagccccatt ttttgtttca gaacttgacg
     1021 ggcttttgaa aggctgatac ctaagttgat catgattcga gtagccatga tactttcatc
     1081 ttttaataag ctcatcaaaa tatgttctgt tccaactttt tgagaaccag ttttcttcac
     1141 ttcgctgcta gctaaagcaa aaacttgttt cattcttggt gaatatggaa gaaagactcc
     1201 tttagggtaa gaacggatac caccataacc aatcagatgt tcaatttctt cataaatttc
     1261 cgtttcattc aaatccattt cacgtaatac ttttccagca attccatttt gctcgaccac
     1321 taaggctagt aaaacatgtt ctgaactaac tgattgatgt ttcaaccgtt ttgcttcttc
     1381 ttgtgcaatt tctaatactg cttgggctct ttcagtaaaa agttcttcca tctaatctca
     1441 cttcccatct tcataactta aacgttccag aaatgctcgg agaatccgag cacgtgtatg
     1501 atcttcatat tcattgaaat tcaatgtgtt ttttgcaatt gcactcaaca tcaagtttcc
     1561 ttcttttttt gtaattattt ggtcttcata aagtttttga ataatagcaa aagcattttt
     1621 ctcactgatt gtatcatcaa ataactgatt tatctgctca agtaattgtt ttttatctga
     1681 aatccttact tttgcaattc gtatataacc accaccacca cgtttgcttt ctactagata
     1741 accacgttgg atggtaaaac gggtattaat cacatagttg atttgtgaag gaacacagtt
     1801 aaagaggtct gccatttcaa tccttcgaat ctcgatcatt tcactatttt ccagaatttt
     1861 ctttatatat gattcaatta aatccgaggt attctgatgt gccatcttat tcattccctt
     1921 tgactaatat tgaccttaat tataacgaat ttgataatag aaagaaagaa gaacgctttt
     1981 tgttaaataa atcctttaga atcaacaaaa aaaaatacgg aatatactat tccgtatc
//
LOCUS       DACCSK010000202         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCSK010000202 DACCSK010000000
VERSION     DACCSK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.211"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IVP39_002763"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IVP39_002763"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7587801.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IVP39_002764"
     CDS             complement(1431..1823)
                     /locus_tag="IVP39_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7587802.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACCSK010000203         2020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCSK010000203 DACCSK010000000
VERSION     DACCSK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.212"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            101..571
                     /locus_tag="IVP39_002765"
     CDS             101..571
                     /locus_tag="IVP39_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7587803.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="IVP39_002766"
     CDS             793..1896
                     /locus_tag="IVP39_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7587804.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          328 c          460 g          545 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac
//
LOCUS       DACCSK010000204         1910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCSK010000204 DACCSK010000000
VERSION     DACCSK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.214"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            492..710
                     /locus_tag="IVP39_002767"
                     /pseudo
     CDS             492..710
                     /locus_tag="IVP39_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            970..1224
                     /locus_tag="IVP39_002768"
     CDS             970..1224
                     /locus_tag="IVP39_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7587805.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1225..1542
                     /locus_tag="IVP39_002769"
     CDS             1225..1542
                     /locus_tag="IVP39_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7587806.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          661 a          300 c          355 g          594 t
ORIGIN      
        1 cattatatca ccaaacccca tcagcgtgca ggctgatggg gtttttttac gtttctatat
       61 tatagaatta acgggtaggc ttactgtctt ttttggcaat tttgcaatca agccatttgc
      121 agaggtaact gcacactaca tctgcaaatg gcttgattgc aaaaacaaaa tcaaaaaggt
      181 atttcattat atctcctcct ccccttgaca gattaggccg gggtagcaga cagcaaaact
      241 attataacat aactgaaata cctaaatgac atatacagat atgataattt atgtattgat
      301 ttttctaaca aaagcacata ttttaatata tactacctat tttaggtagt atatattttt
      361 agatacaata tcgatcagtt ttgaggaaat gaaattctag atattttttg ctaaatagag
      421 ttactttcaa tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg
      481 atgaaattgc cattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt
      541 ggtctaatgg atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa
      601 ataaagttat tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga
      661 acactcgcaa ctttattcgt gtagatgaat cggcttcggt tgagttataa ctttagctat
      721 atcaatcaac cgaccgacat atgttaaatc tatatataat aaaaatacct acttaaatgg
      781 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
      841 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
      901 acaccgttgg caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag
      961 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
     1021 cttccaagct ctcttggatt aaaaagcgga gacactatcc aagccaaaca agaaggcaac
     1081 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
     1141 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
     1201 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttc
     1261 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct gtctccagcc
     1321 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
     1381 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
     1441 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
     1501 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt
     1561 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
     1621 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
     1681 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc
     1741 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     1801 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     1861 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttgt
//
LOCUS       DACCSK010000205         1889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCSK010000205 DACCSK010000000
VERSION     DACCSK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.215"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..256
                     /locus_tag="IVP39_002770"
     CDS             <1..256
                     /locus_tag="IVP39_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7587807.1"
                     /translation="FYIEDIAVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDN
                     NLIACKFYHNCGFKIGSVDTMLYANFENNFEKAVFWYLRF"
     gene            349..1143
                     /locus_tag="IVP39_002771"
                     /note="aph(3')-IIIa"
     CDS             349..1143
                     /locus_tag="IVP39_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7587808.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1553..1664
                     /locus_tag="IVP39_002772"
                     /pseudo
     CDS             1553..1664
                     /locus_tag="IVP39_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1685..1768
                     /locus_tag="IVP39_002773"
     CDS             1685..1768
                     /locus_tag="IVP39_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP7587809.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
BASE COUNT          608 a          293 c          421 g          567 t
ORIGIN      
        1 cttttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc
       61 gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga
      121 aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg
      181 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
      241 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc
      301 ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg
      361 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
      421 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
      481 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
      541 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
      601 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
      661 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
      721 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
      781 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca
      841 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
      901 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
      961 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
     1021 gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
     1081 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
     1141 tagtacctag atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct
     1201 tttctgaagt acatccgcaa ctgtccatac tctgatgttt tatatctttt ctaaaagttc
     1261 gctagatagg ggtcccgagc gcctacgagg aatttgtatc gataagaaat agatttaaaa
     1321 atttcgctgt tattttgtac atttaacttg acggtgacat ctctctattg tgagttatta
     1381 gtggtacagt tttcaaccgt tttaattata aaaaagtggt gcatttttaa attggcacaa
     1441 acaggtaacg gttattgcag gtgtatttct tatctatggg tttaacatgg attttatcat
     1501 taaaatcatg agtattgtcc gagagtgatt ggtcttgcgt atggttaacc ctaaagttat
     1561 ggaaataaga cttagaagca aacttaagag tgtgttgata gtgcattatc ttaaaatttt
     1621 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt
     1681 cgtcatgttg gtattccaaa tgcgttatgt agataaaaca tctactgttt tgaaacagac
     1741 taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat
     1801 cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtatttg
     1861 tttatagatg taatcacttc aggagtgat
//
LOCUS       DACCSK010000206         1888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCSK010000206 DACCSK010000000
VERSION     DACCSK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.216"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            6..398
                     /locus_tag="IVP39_002774"
     CDS             6..398
                     /locus_tag="IVP39_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7587810.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..1838
                     /locus_tag="IVP39_002775"
                     /note="aph(2'')-Ia"
     CDS             399..1838
                     /locus_tag="IVP39_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7587811.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          787 a          138 c          322 g          641 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg
     1381 tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa
     1441 aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa
     1501 agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc aataatagat
     1561 taactggaat aattgatttt ggagattctg gaattataga tgaatattgt gattttatat
     1621 acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa
     1681 tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt
     1741 atcctattga aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg
     1801 gtagaaaaga aatttataaa aggacttata aagattgatt atataatata tgaaaagcta
     1861 ttataaaaga cattagtatt aaatagtt
//
LOCUS       DACCSK010000207         1832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCSK010000207 DACCSK010000000
VERSION     DACCSK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.217"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            32..862
                     /locus_tag="IVP39_002776"
     CDS             32..862
                     /locus_tag="IVP39_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7587812.1"
                     /translation="MSLLIYTHVLSTFMSVIVIGFLFIGSLFSKRKEKKEMSDLILNM
                     IKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTNLQERALNISDSLIGAVNNDLVTFT
                     MGTIGVVSLILPLFFLKKFTNKEKIIYFCAVTTWFLSTNLFPWEILQKTPIQIIQFPW
                     RILGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAATEANYSHKIQSYK
                     GRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWRMHLIKHIIPK
                     "
BASE COUNT          654 a          250 c          288 g          640 t
ORIGIN      
        1 aaagaactgt gtgattcctt tagctatcag tatgagtttg ctaatctata cccatgttct
       61 ctccactttt atgagtgtga ttgttattgg cttcttgttt atcggctctc ttttctctaa
      121 gcgaaaagag aaaaaagaaa tgtcggatct gatattaaat atgataaaag ctgttggttg
      181 gaccattctt ttaacggctt acgtttggta tccgatgtta caacaagtcc tacatcaaaa
      241 aattaatcgt ccttttcgca caaatcttca agaaagggca ttgaatattt ctgatagttt
      301 aatcggtgca gtaaataatg atttagttac gtttacaatg ggaacaattg gtgtggtttc
      361 attgattctt ccacttttct ttttaaaaaa atttaccaat aaagaaaaga ttatttattt
      421 ttgtgcagtg accacttggt ttttatcaac gaatcttttt ccatgggaaa ttttacaaaa
      481 aacacctatt caaatcattc aatttccgtg gagaatcttg ggttttcaag tgctttttgg
      541 ttcattaatc ttaaccatag tgtttttaaa gtggaaaaca agcaacaaaa aaagtatgtg
      601 gagccttgta ggaatagttc ttattatttt tactgtgact gcagcaacag aagcaaatta
      661 ttcgcataaa attcagtcgt ataaaggtcg tttagttatg accaaaaaag atgttacacg
      721 ttatacatcc tcaagaactg gaggcctata tgactatgca ccattagatg cattgaaata
      781 taaagatcat ttaaagaaac atgaagtaaa agttggtgat cgatggagaa tgcatctcat
      841 aaagcatata attccaaaat agtctataca gtagaagatg ccgatgagca aaatatcaca
      901 ttacctgtat tcaattattg gggaacagtt gtgaaagtaa atgggaaaaa gagcactata
      961 gaaaatgaca atggtttagt gtctattaaa ggtacgggaa aaagaactat agttgaggtt
     1021 tcatctacgt atccaactac gatgattcta tctttattaa ttagttcaat aacttttata
     1081 ttcttattta cacgttattt aataagatgc tgcggttaaa tgaaaataga ttagatagat
     1141 attataaata tgttgattct attttcatag caatggagaa aatacattag caatatataa
     1201 aagaaggtat caagagtcac agtgaattta ttccgaaaag tgaaaagagc aaatataaaa
     1261 tttaataaaa atatagaact aaataggaaa acgtagacgt aattttctac gttttccttt
     1321 tcatatatat taaatattac ggaaaattat ataaactaat ttagtgtttt gtttgtaatt
     1381 cctaattttg ctccactttt agataatttt taggtctatt taactcatca gacattctat
     1441 gctcattttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     1501 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     1561 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     1621 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     1681 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     1741 aagaactagt agaagaagga atgatggctt gataataaag tactacctat taaaggtagt
     1801 actttattta atttagtata aattaacata ct
//
LOCUS       DACCSK010000208         1774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCSK010000208 DACCSK010000000
VERSION     DACCSK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.218"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            197..424
                     /locus_tag="IVP39_002777"
     CDS             197..424
                     /locus_tag="IVP39_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7587813.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            547..873
                     /locus_tag="IVP39_002778"
     CDS             547..873
                     /locus_tag="IVP39_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7587814.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            941..1654
                     /locus_tag="IVP39_002779"
     CDS             941..1654
                     /locus_tag="IVP39_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7587815.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          686 a          273 c          374 g          441 t
ORIGIN      
        1 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
       61 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
      121 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
      181 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
      241 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
      301 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
      361 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
      421 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
      481 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
      541 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
      601 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
      661 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
      721 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
      781 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
      841 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
      901 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
      961 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     1021 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     1081 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
     1141 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
     1201 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
     1261 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
     1321 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
     1381 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
     1441 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
     1501 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
     1561 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
     1621 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
     1681 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
     1741 aacgcaaaga ggtgcatctc tgatttggtt gttt
//
LOCUS       DACCSK010000209         1774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCSK010000209 DACCSK010000000
VERSION     DACCSK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.219"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            287..1771
                     /gene="guaB"
                     /locus_tag="IVP39_002780"
     CDS             287..1771
                     /gene="guaB"
                     /locus_tag="IVP39_002780"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7587816.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          603 a          313 c          402 g          456 t
ORIGIN      
        1 aactaagcag ctttttcaca gcctctccac taaacggtgt tcaaaagttt gcatctgcgg
       61 taataaaccc aaccaattca aaaacatgag aagctgtttt tgattggctg ttcttattac
      121 ttgatgcagg acagcttttt tacaatctct tttaatatac acagatgtaa acgctttata
      181 ttggtattct atgagaaagc taacttgatt ccaagtagat cattgactta aatgaaaaaa
      241 tcaattattt taatagataa actaaattag aggagtggta ctaaaaatgt ccaactggga
      301 aacaaagttc acgaaaaaag gatttacttt tgatgatgtc ttattgatac cagcagaaag
      361 tcacgtgtta ccaaacgaag tggatatgag cgtacaatta gctaaaaata ttaagttgaa
      421 tattccaatc atcagtgcaa gtatggatac tgttaccgac agcaagatgg ccattgctat
      481 ggcaagacaa ggcggactag gtgtgatcca taaaaacatg acgatcagcc agcaagcgga
      541 tgaagtaaga aaagtcaaac gttctgaaag tggcgtaatc attgatccgt tcttcttaac
      601 gccacagcat cttgtagcag acgcagaaga attgatgagc aaatatcgta tcagtggtgt
      661 gccaatcgtt gaaacacttg aaaacagaaa attggtaggg ataattacaa accgcgacat
      721 gcgttttgta acagattatc acatgccgat tgctgatgtg atgacaaaag acaacttagt
      781 gactgcccca gtaggtacct ctctaaaaga tgcagaaaag attttgcaaa aacataaaat
      841 cgaaaaatta ccaatcgtag ataacgaagg tcgtttaagc ggattgatca cgatcaaaga
      901 catcgagaaa gtgatcgaat ttccaaatgc agcaaaagat gaacatggtc gattgcttgt
      961 agcagcagcc gttggtgtaa caagcgatac atttgagcgt gcgaatgctt tattagaagc
     1021 aggggcagat gcaatcatca tcgatacagc tcatggacat agtgcaggtg tgatccgaaa
     1081 aattcaagaa atccgttcaa cttttgcaga tgcaacattg attgcaggaa acgttgcgac
     1141 ggctgaagcg acaaaagctt tatatgatgc aggtgtagat gtcgtaaaag ttggtatcgg
     1201 accaggatcg atttgtacga cacgtgtggt agccggtgta ggtgtacctc agctcacagc
     1261 tatttatgat gcagcatcag ttgctcgtca atatggcaaa gcgattattg cagacggagg
     1321 gatcaaatat tctggtgaca tcgtcaaagc attagctgcc ggcggacatg cagtgatgct
     1381 aggaagtatg ttggcaggga cagatgaatc accaggtgaa ttcgaaatct tccaaggacg
     1441 tcgattcaaa acataccgag gtatgggctc attaggcgcg atggaaaaag gttcaaaaga
     1501 ccgttacttc caaggtagtg tcaatgaagc aaataaatta gtaccagaag gaatcgaagg
     1561 tcgggttgct tataaaggaa gtgttgcaga tattattttc caaatgatcg gcggtttgcg
     1621 ttcaggaatg ggttatgttg gggcagccaa tctacagcaa ttacgtgatg aagcgcaatt
     1681 tatccaaatg agcggcagtg gattaaaaga atcacaccct catgatgtac aaatcacaaa
     1741 agaagcacca aactattcag ttgaatctta aaaa
//
LOCUS       DACCSK010000210         1768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCSK010000210 DACCSK010000000
VERSION     DACCSK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.220"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(86..1234)
                     /gene="tnpB"
                     /locus_tag="IVP39_002781"
     CDS             complement(86..1234)
                     /gene="tnpB"
                     /locus_tag="IVP39_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7587817.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1251..1655)
                     /locus_tag="IVP39_002782"
     CDS             complement(1251..1655)
                     /locus_tag="IVP39_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7587818.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          527 a          311 c          276 g          654 t
ORIGIN      
        1 cttgatagcg tatttcttta ctaacagcgg tgtctcttat ttaccaagct atccccgtag
       61 ttcctacggt tcttgatgat tggatctaag ccaatcccat agaatgtagt cttagacctt
      121 cggtcagaat attgatacta gcattgatat cacgatcatg atgagtatga caaacaggac
      181 aagtccattc tcgaatttca agagatttct tgccatcttt atgtccgcac tctgaacaaa
      241 tctgactaga cggaaaccat ttatctactt tgatgatttt tcgtccatac cagtcagcct
      301 tgtattgtaa tttagtcaca aactttgacc aagaaacatc agaaatactt ttagctaatt
      361 tatgatttcg caacatacct tttgtgttta agtcttcaat acagataata tcgtgatttt
      421 tgatcatttc tgtactcaac ttattcagaa aatcagtacg ttgattcatt actttttcat
      481 gcaatctagc tactttgcgt ttttgttttt gataattttt agcttcaaat agattggtac
      541 ctttattttt agccaataaa gcacgcctag acaatttgcg ttgttcacgc tttagtttct
      601 tttccatttt ggacgtgaat ttattattat caattttttg tccatcagaa agaatcgcaa
      661 agtccataat acctaagtca ataccaattg tggaattagt tttaggtaat tcgataattt
      721 cttctttaca caacaaagaa atatagtatt taccgctaga atgacgtgat attgtagcag
      781 atttgataat cccttttggt tgtctatgaa gcttaattct tactaatgac ttcaatttag
      841 gaactttgat gaatttacta tcaatcaaag caactgtacc attttgatta ttcgttgtat
      901 aactctgaac aggatttttc ttacttttga aacgtggaaa tccaacggat ttatcccgaa
      961 aaaaattctt gtatgcttta tctaaattaa gttgggcatt ggctaaagca aggctatcaa
     1021 tttctttcaa aaacggaaat tctttcttgt atttagctgg tgtcggaaaa gtcatttttt
     1081 tagaagaatc atttttaact tcttcatacg ccttttttcg gtcatcaagc attagattgt
     1141 agaccttacg gacacaacca aaagatttgg caaagaagat ttcttgttcc tctgttggat
     1201 agattctgaa tttgtatgct tttagttgtt ccataaagtt cacctacttt ctatttatga
     1261 ttgtttcctt gattttggat atacttctta atcacatcaa ttggtgcgcc accagttgtc
     1321 agaagacaaa aacttttaga ccagaacatt tctttccaaa gaaattgttt gactcttgga
     1381 aagtcacgtt ttatcaatct tgagctggca cttttatagg cattgatgaa ttttgtcaat
     1441 tctgttttag gttgagcttt gaacataata tgaacgtggt ctttatcatg attccactcc
     1501 actaaagtaa tgtgatacga ttctgaaatt ctttcaaaag tagttttggc ataatcagat
     1561 atttcatcat caatcacttg tctacgatat ttcgttacta aaacaaggtg gtaataaaga
     1621 agaaatactg aatgattatt tgtatctaat tccatgttat atcaactcat ttcttgtata
     1681 gactgattgt aacatggaac aaaataaaaa ggcaatacgc cttagttgtc ggtcttcgag
     1741 ttaccaacgg taaccctcat accgaacg
//
LOCUS       DACCSK010000211         1697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCSK010000211 DACCSK010000000
VERSION     DACCSK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.221"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..514)
                     /locus_tag="IVP39_002783"
     CDS             complement(<1..514)
                     /locus_tag="IVP39_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7587819.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLS"
     gene            complement(684..1643)
                     /locus_tag="IVP39_002784"
     CDS             complement(684..1643)
                     /locus_tag="IVP39_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7587820.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT          464 a          331 c          314 g          588 t
ORIGIN      
        1 tactcaaccc agttacagtt tgactagttg gtccagtgga ttcgttatct gacgtacttt
       61 taacagtgga tgttccaaaa tctaattcag cgccttgcaa aacttgtgaa ccgtttacaa
      121 aatctgaaac cgaaactgtc acgttccacc cttgtgcttg gccatctgca cctacacctc
      181 gacgatctga tacttggaga taaggtgtat cgttttttga agcgtctact tgatacgttt
      241 ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc gggaactaca tccagtgtta
      301 aaggacctgt ctggccagtt gctggatttt ccggatctgc tggctctact ggttttgaag
      361 gatcagtagg gttaagaggc tccacggtat ttgttggttc agtaaaggta atatctcctt
      421 ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa gccgcctaga agaagtgttg
      481 aaagtaataa tgtcccaatc atgttctttt tcataataaa tcactccttt ttcttagtga
      541 cctaagtata gtagagacag tatgcaaaaa atcctcatga attcatccgc ttacttgaaa
      601 ttatgaccct ttttgtataa aagagaaaaa aacaataaaa tcgtttacat gaatattctt
      661 ccttttttat taagtttcgt ttgtcaaatt aagttagaca aatcatcttt tgtgacgtaa
      721 ctcaaaaata cttccattgg tgtccgatag tgtaatgatt ttctaggaat atgatttcgt
      781 ttcgatgcca ccgattggat aaaggtttca gatacaccat taaaatccat agctttatac
      841 aaaccatctt tacgtaataa gccatttgaa tgttcattca atcctcgttg agaaggtgtt
      901 cctggatccg caaaataaat tgaaatatcg ttggtattac taatcttttt ccaatttgag
      961 aattcttttc cacaatcaaa tgtgatggat ttaaataagt tttttgggac acaacgaaac
     1021 cattgattca gcctattttc aatgtcttta gcctgtctgc cttcgggttt caaggtaatg
     1081 atgactttag atagccgttc aactaatgtg atgaccgcgc ttttatgttt tccacccacg
     1141 atagtgtcac cttccaggtg accaaattca tgatcaaact gggtatagtc tgtctttctg
     1201 tctcggattg accggcgaaa cccttgtttt cctctttttt ctttatgacc gtttggtttt
     1261 ctttttccct tcattggtaa atcgtgagtg tcaaaggttt tatctttaaa cattcgatac
     1321 agtgtgcgta ccgaacaagc aataggaaac tctgctcgac caataaggac atcaggagcc
     1381 cacccttgaa caaccttttt ttgaatgtat tcctgttgct catcaggtaa aacaatcgga
     1441 tgtcttccgc agttacgttt gtttttctta tattgtttat agtagctcat tgcgcttcca
     1501 ccagatttaa gaaaacgata gaccttataa atagtttgtc gagaacgttg taacagattg
     1561 gctacttttg aaacaggttg tgattgattg aaataagctt ctatcattac gagttcagtt
     1621 ggtgtaagat ggttataggt catttgtact cactccttat aattctttcg tcggaactat
     1681 tttgagtgta gcacaaa
//
LOCUS       DACCSK010000212         1685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCSK010000212 DACCSK010000000
VERSION     DACCSK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.222"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..719
                     /gene="erm(B)"
                     /locus_tag="IVP39_002785"
     CDS             <1..719
                     /gene="erm(B)"
                     /locus_tag="IVP39_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7587821.1"
                     /translation="SQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKIS
                     KQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQI
                     IKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPK
                     VNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLST
                     VTYEQVLSIFNSYLLFNGRK"
     gene            complement(982..>1685)
                     /locus_tag="IVP39_002786"
     CDS             complement(982..>1685)
                     /locus_tag="IVP39_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7587822.1"
                     /translation="EETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRM
                     TEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTI
                     ERYETMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSS
                     LETPSISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQ
                     PPTPPIPKTPKLPGI"
BASE COUNT          520 a          290 c          291 g          584 t
ORIGIN      
        1 attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa caattgaatt
       61 taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta acgacgaaac
      121 tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta ttcaacttat
      181 cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat attctacagt
      241 ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac catttaagca
      301 cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc tgacatctat ctgattgttg
      361 aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg ctcttgcaca
      421 ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat cctaaaccaa
      481 aagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca gataaatatt
      541 ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt caactgttta
      601 ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta agtaccgtta
      661 cttatgagca agtattgtct atttttaata gttatctatt atttaacggg aggaaataat
      721 tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa tgtagataaa
      781 ttattaggta tagggcacct ctaataacta ccaattaatt cacctcaaaa tgaaattaga
      841 aaaaaacagc gaaattttta aatctatttc ttatcgatac aaattcctcg taggcgctcg
      901 ggaccccaac atgaggaaat gtttttataa aaaaagagta gttaccgttt tttggtaact
      961 actcttttcc ttttttaagg tttaaatacc tggaagttta ggtgttttgg gtattggtgg
     1021 tgtaggtggc tgcaagcttt ccaatttttg ttgaattgct ttaaattcag gtgtctcttg
     1081 gtgtttattt agaaccattt tttgctctat tcgttctaaa gtcggttgaa tttctttccc
     1141 tgatacttta cgattcaatt ctttttctaa ggtttctttt ggactaatag aaggtgtttc
     1201 caaacttgaa tagagtttta ctccttctct gttatatagc cttatatcgc taaataagcc
     1261 cgttttatga agggtttcta aattggtcgg taagtttttg acaacaatat catgcgcttg
     1321 ttttggtgtt gccctggctg tcattggatc atctgcatac atggtttcat atcgttcaat
     1381 tgttcctaaa tatgagttga ttttaggtac tgccatgaca tacatttttg tttcataacc
     1441 tttggcttga agcattgttg cggtttgaat aggaacgtct gttgttcgtc ctgtaccttc
     1501 gatcaccaaa ttataccctt gatcgctcaa acggcttatg atcgcttctg tcatgcgatt
     1561 agaataaggg gtaacgtgtt ttactacgtc tttttcataa agtttcacta gttcatcaaa
     1621 attagggtgc tgttgtttaa aggtatcatt atcaatgaca ataacattcc cttgtgtttc
     1681 ttcaa
//
LOCUS       DACCSK010000213         1638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCSK010000213 DACCSK010000000
VERSION     DACCSK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.223"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(185..1504)
                     /locus_tag="IVP39_002787"
     CDS             complement(185..1504)
                     /locus_tag="IVP39_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP7587823.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          486 a          301 c          325 g          526 t
ORIGIN      
        1 taattctttg gcacatttta ttgaaatatg tatcactaat atccctatca actgattttg
       61 actaaaaagt tacttcaaac attcactgtt caaaaatatt gaggaataaa ttttgggtgt
      121 aaggcatttt gagcatactt ctccctggat aacttcccag taaaaaaatc gttagattgt
      181 tggattagat ccattgactg gctcgcctca ggcgagcaaa gaccctgtag aattcattct
      241 gatacacatg ataactagat aatttgagat agactcgtct acccgtttgg actaatttcc
      301 cagcaacttt cagaaacttc aatcgaatag aatgaatggt cattcccttt tggacttctt
      361 caaagccaat cgttcttaag aagttgacta agttgtaagc tatcaagctg agcatcattc
      421 ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc aaagtaaaag ccagctttcg
      481 cttctttaat gaagttctcc attgtgccac gtttggcata gatagaaaag atggtatcag
      541 gagaaacatt ttctgataga ttcgtcacga taaattcatg tcgaaagagt agttcgcccg
      601 cttcacgtgt tgagcgtata cacacgcgac gactttgtga ccaggattgt gcttgataag
      661 tggtcgagaa gtactgaact tctcgttctt cccacttttg attatcgcca taaagtactg
      721 atttctcagc tatttgacct agtctacgat tattcttcag tcgaataaca taatgactct
      781 tttttgattc acacgaatca tacacaccag gtgtagcgaa cccgctgtct ccacgaacca
      841 agatgtcagt gtttggtaga gagtgattat agtgctctaa taaaggtgtg agaaactcct
      901 ttacgccttt tgatgtatat tgatttcctg aacgtagttc agcttttaag aagtcaccag
      961 tcaatccgtc aaaagctacc aatggatggt aaccataggt ttggtagtgt gtattataat
     1021 tcgtttgttc ttggtgacca aatgtatctg aatgggtcga atctaaatca atgattaatt
     1081 ccgtatcatt acggatgagg cgtgctttat cgataagttc ttggttcaaa gcttgaagtt
     1141 catggatatt ctcctcagat agtcgatcta aaaatcggga aagtgaagat tgagaagcga
     1201 gttctttcct atctaaaaca gctttaaaca caggatcttg tctcaggaga ttagctgcag
     1261 aatcagctga gtaaccagca attaattgca taatgaactg ctcaagtatg gataagttgt
     1321 catgagtaaa atacgctcgt tcgtcttcaa tgtgaatgtg ttgcttagcc agttcagaaa
     1381 aaccaaagat gttcatcagc tctttcacta ggacgagacc cgaatcactc gataattgac
     1441 cacctgtatg agaaatagtg atatttgaat tgaattttac ttggtttttg tgtaagctag
     1501 tcatgaaaag aactcctttc ttttggttgg tttagtcact ttaaccatag cagaaagggg
     1561 ttcttttttc atcacttaac gggtgaagat gaaaaagtaa ttgtaagctg ccgtgaggca
     1621 gtttcacatg gttttaat
//
LOCUS       DACCSK010000214         1632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCSK010000214 DACCSK010000000
VERSION     DACCSK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.224"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            326..563
                     /locus_tag="IVP39_002788"
                     /pseudo
     CDS             326..563
                     /locus_tag="IVP39_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(707..1483)
                     /locus_tag="IVP39_002789"
     CDS             complement(707..1483)
                     /locus_tag="IVP39_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7587824.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT          521 a          283 c          248 g          580 t
ORIGIN      
        1 ggtcagctga ttgaacaccg tttaagaaag aggtcgtgag cctgtcattt aactctgagt
       61 cacaagtaac aatttcaaaa aacagcttct catctttgat gaatatcgtg acttgtggac
      121 gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc aaaaaacgat
      181 ggaacgttgt tttgattgcc cttaatgagg acagtcggaa caggtttgtc atttttatat
      241 ttaaatggca caaatttttc taattatttg tcatttagcg ctgaaaattt ttgctattta
      301 tctaaaaaag tgataactac ttaagatgaa gcagaatatt tacgccatac ggcatacaac
      361 cgcgaaaaat ataaaaaaaa gaaaacgatt gaacgcgttt ttgcagtcgc aaaggaaaag
      421 catggaatgc gctacatcaa gtatcgagac ttggaaaaca atcaggtgta tacgctgatt
      481 gtttttgctg caatgaatct gaaaaagttg tccacttggc agttggaaca tatgtcttca
      541 tatcttttat tatacaaaaa tagcaaaagc accagtctac actcttagta aagaggaaac
      601 tgatcctttt gtctacattc ttaaagtact taaactaagt actttatttt actctttagg
      661 attgatacag tagcatctat ccctttatta ataatctttt atttttttaa ataaagatgt
      721 tgtctccatc ttcaggcact aaaacttttt cggaaattcc tttttcttta ttgaaagact
      781 ttaattcttc tcttgacagt ccccaatggt ttacagcttc catatgaacg gcaataattg
      841 tggaatctgg tattgcttta tgcaattcat ataaatcgtc tttccccata ataagagatt
      901 ctccaactaa aaaatgatta tctccagcat ttgctactat gatttctgga ttatactgat
      961 caatagtttg tttaacagct tcataccaaa ctgtatctcc tgtaaagtat agtgtcttct
     1021 cagtcgaatg tttgaaaaca ataccgcaaa cattacctac ataattcaag atttctcctc
     1081 tgccatgttt gccttttgtt ttaaccaatt gtatagattc aaaaactgtg tcttctcgta
     1141 gtacctccac atttttaaaa ccatatagtc gaatttcatt cgcatcattt tcatcttgca
     1201 caaataattt tatttcttta ggtaggacct ctttcgctat atcgtcccaa tgatctagat
     1261 gtaaatgtgt tacaataaca gcatcaatac cgttgataat atcttcgact ggcattggca
     1321 aatcaactag aggattcatt tgatcttttc ttggtgatgg aaaaggacct ttcgaaataa
     1381 atggtggaag tgttcctttt tccgctaaca ttggatcaat taaaaatttt ttacctccat
     1441 agtctaagat agctgttgca ttacgaatgt gatgtatatt cataaacgtt gtctcctttt
     1501 tgttgatatg aaatattttt agtataattc aattccatac aataaataaa ccgaacaaaa
     1561 aaagtctttt actattttta cttaatttat gaaaggttgt ttaccatgtt agaccaaaca
     1621 gatataataa at
//
LOCUS       DACCSK010000215         1626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCSK010000215 DACCSK010000000
VERSION     DACCSK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.225"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(364..1605)
                     /locus_tag="IVP39_002790"
     CDS             complement(364..1605)
                     /locus_tag="IVP39_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7587825.1"
                     /translation="MNAYQPESVDDMQDALKDVFGPLFEKMLQGELNNHLGYDAHSKE
                     PKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIPKRKRDVSDIEGKVLSMY
                     ARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQARPLAKCYAFLFVDCMY
                     VTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNRWMQVFDELKARGVEDVF
                     FISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSKDYKEVCRDMKKFYGASS
                     LNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSAIRKIMYTTNAVESVHSS
                     FRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAMVLNQLMINETFSSRMKS
                     MRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          507 a          330 c          276 g          513 t
ORIGIN      
        1 atgggctact ctgtgatact tattagccat taaactattt tacccacata gcatatattt
       61 tgaaatcgtt cattccgtaa attcaactaa atatgcctaa aaaaataaaa aatctaagat
      121 agaataagat ttattcagtc ttagatggat ttatagcaac tttttcgtaa caactttaaa
      181 aaatacgaaa agaaagagct gttttactct gactacgttg ctaaattgtc gtttaatcat
      241 tcatttaaac caaatctttt tttatagaat tcgttttaat cctcctgaat ccaaaaacta
      301 tgaaaaccgt aattaaatag ttaagagttt ttaaccacca taattctctc ggctattttg
      361 aaatcagata aattttgatc ggacacatag ttgaataaac ctaccgttgt tacggaaggt
      421 aaatcgcata cttttcattc tagaggagaa ggtctcgttg atcattaact gattaagcac
      481 catagcccag ttttgaattc gaccacctga ccacttcgcg tgtaattctt tcgtgcgtaa
      541 ataaagtagt tttaggagtg cattctcatt agagaacgct cctttttttg tgacttttct
      601 gaagctggag tggacacttt caaccgcatt ggtagtgtac ataatttttc gaatggcact
      661 accataatca aatagttgtt caacatgtgc aaagttccgt ttccagacat ctacagcacc
      721 agaataatga gaccaccgat tttgaaaact gtcaaaagca gcatgtgcag cgtttagaga
      781 agaagcaccg tagaactttt tcatatctcg gcagacttcc ttatagtcct tacttggaat
      841 atagcgcaat gcatttcgaa caagatgaac aatacagcgc tgaacaatta ccgatggaaa
      901 gatcgctttt gcgccttctt cgaggccaga aacaccgtcc atcgaaatga aaaagacatc
      961 ttcgacacca cgtgctttca gttcatcaaa tacttgcatc cagcgatttt tagattctgt
     1021 ttgatttaac cataatccta aaatctcctt atttcctttg agatcatagc caagaatggt
     1081 gtatacagca tattctttgg cttcataatt ttctcgtaaa gtaacataca tacaatcaac
     1141 gaatagaaag gcataacact ttgctagggg gcgggcttgc cattcttcca attcaggaag
     1201 gacagcgtca gtgatatctg aaatcatttc atgggaaata tcaaagccat aaatagcttc
     1261 gacggttgcg gcaatatctc gttgactcat tcctcgtgca tacatggaaa gaaccttccc
     1321 ttcgatgtcg gagacatctc gttttctctt aggaattaac tctggttcaa aggaagcttc
     1381 ccggtctcta ggaacatcaa tagctacttc accaaaactg gttttaagcg ttttagttcc
     1441 atagccattt cgacggttat cgtgttcctt aggctcttta gaatgggcat cataacctaa
     1501 atgattattc aattctcctt gaagcatttt ttcaaaaagg ggcccaaaca catctttcaa
     1561 agcatcttgc atgtcatcga cagattcagg ttgataggca ttcagaatgg attcagctaa
     1621 cttttc
//
LOCUS       DACCSK010000216         1599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCSK010000216 DACCSK010000000
VERSION     DACCSK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.226"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            76..1395
                     /locus_tag="IVP39_002791"
     CDS             76..1395
                     /locus_tag="IVP39_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP7587826.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            1367..1564
                     /locus_tag="IVP39_002792"
     CDS             1367..1564
                     /locus_tag="IVP39_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587827.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
BASE COUNT          573 a          244 c          360 g          422 t
ORIGIN      
        1 gttaagggct aaaaaggctg cactaccagt cttttagccc ttttcatttt gtttttgaac
       61 aagtaaaata ttactatgaa agcgaatatt ctcgaagcaa tcttttttga cgaacaccat
      121 aattgggata actttgttga gaaatacggg cgaaagattc ggctagtcgt caaacgggaa
      181 gtaaacaagt ttagatattg tggagatatt caaaaaggat accgactgtt tgtctgcgag
      241 ggatgtcatc aaacaaagtt tgttcccttg aaatgtcgtg gaaagttctg tccaacgtgt
      301 gcaacaggtg aaagccaaag gtggtcagcg gttgtggcga atgatatgtt taaagtgaca
      361 catcggcacg taatctttac gatcgatgag gggttacggg aaatctttat gatggaaaac
      421 tatcgagatt ccttactgaa aggactaatg gatgaagccg caaaaattat tcttgcctat
      481 tttgagaaaa gaaaggtgaa ggcaggtata gtagctacaa tccacacctt tggatcaaaa
      541 ctagaattta atccgcatgt tcatatggtt gttactatgg gtggcttaac aagagatgga
      601 agttgggaag aatacgatta tttacctttt tctatgttaa gaaaatactg gaaaaatgct
      661 gtattgaaac taattagacg aacgttaggt gagtgggaca agcaaagagt tcaatcaaga
      721 ttacaagctg cgtacaagaa caatggagaa gggttttacg taaatgctcc taaaaggagc
      781 aggacaaatc taaagtcact actccaatac atcagtcgtt atatgaagcg tggcccgatc
      841 gcattaagcc gaatcttaat gtatgatggc gacactgtca tgttcaacta catggacaaa
      901 cgaacgaata ctaaagagac gatgaccatg agtatagacg aattcatagc tgcactgatt
      961 cgccatattc aaaataagaa tttcaaaacg attcgtcgct atggaatcta tagtcgtaga
     1021 atcaaaacat taatgaaaaa ggtcatgaag gagtatcaga aacgagtaaa aaagctgttt
     1081 atcaatctga atgaggcctt aagaccaaaa tcctgggaag aaagaataac cgaagaattt
     1141 ggtgaaaatc ccttagaatg ttcttgttgc ggtgagtact atgaattcgt tgggatgagt
     1201 gtgcgcaaaa atggacggtt agttattaaa tatgcgaaag ataaccatgc aaagcggttt
     1261 atgcgagagg agaatcgaaa aattgaccaa aaagaattca ggcaacagta tgaagaagaa
     1321 aaagaaaaaa cttttaaagc aattcgctgg gattgggaac gccaacgtga aatttatatg
     1381 tctaagttgt ggtgaaaaag aatcaatccc aatggatgta gtaaaatttt tagatgatgc
     1441 agatattgca aatgatccga acaatcctcc ccaatttacc tgtgaaaaat gtgggaagga
     1501 gatgtatcca gaatattata gaaacgccct tggaatagag tttaagatca gtgatgttca
     1561 ataagaaggt taatcataca aaaagtccac cggagaatt
//
LOCUS       DACCSK010000217         1579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCSK010000217 DACCSK010000000
VERSION     DACCSK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.227"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1116
                     /locus_tag="IVP39_002793"
     CDS             <1..1116
                     /locus_tag="IVP39_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7587828.1"
                     /translation="NYLALRIANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLK
                     EDQLLKQNLRSHLSSTYFRLHYGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENF
                     YVPQEETAYLTIHFQAAIERHKHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTI
                     MDLVEYKPELKRQDFPSVDFILATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVP
                     IKKKKTFDLASFTHPFLIFPQLERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSH
                     SSTRVAPFVTIPHGNPLYVKHSMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEF
                     KKIFSVIHYLEKQPEQMAQLMQSNHPLEILTLLSSYE"
     gene            complement(1396..>1579)
                     /locus_tag="IVP39_002794"
     CDS             complement(1396..>1579)
                     /locus_tag="IVP39_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7587829.1"
                     /translation="CGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSEEEILTEFRKVRE
                     GIKRKVLDLNQRTSIV"
BASE COUNT          507 a          284 c          274 g          514 t
ORIGIN      
        1 aattatctgg ctttgcgtat cgccaatctt tttacaaatt cacaagcagc cacacgtttt
       61 caaaaggaat ctagcgcact tgctgatcat ttgatcgaac aagtggaaca gtttctaggc
      121 tattcattaa aagaagatca gctactaaaa caaaatttaa ggtctcattt atcttcaact
      181 tattttcgac tccattatgg tttgcagatt tctaatccat tgacgaaaaa tgtgtttagt
      241 acgtacacac aacttttctt agttttacag ttaattttag aagattattt tgaaaaggaa
      301 aatttttatg ttccacaaga ggagacagct tatttaacga tccatttcca agcagcgatc
      361 gagcgacaca aacatcagaa aagtagaaga tatcaaacgg ttttagttag tgaatattca
      421 aaagctatgg ctacttttct cgaagcacgg ctgaacagag aacttccaga attaacgatt
      481 atggatctag ttgaatataa gccagaatta aagagacaag attttccctc tgttgatttt
      541 atcttggcta cgttaccatt taaacatata agcattccag taatcgagat ctcaccaatg
      601 atcacagaaa ctgatctagc ctatttgaca aaatatatgc tggagcatgt gcctatcaag
      661 aagaaaaaaa catttgatct agcaagcttt actcatccat ttctgatctt tcctcaactt
      721 gaacggacag acccagccga tattttaaac tttatgggaa acgtcttagt ggaacatcat
      781 tatgtggagc cggaattcat cgattctgtc ttggaaagag acagtcactc cagtactaga
      841 gtagcaccat ttgtaacaat ccctcacggt aatccgctgt atgtgaagca ttcgatgatt
      901 tctattgcta caatgaaaga accagtccta tggcacgggg aacagatacg tatcgtactg
      961 ctattagcaa taaagaaaaa tgatttgaaa catgcagagt tcaaaaaaat attttctgtt
     1021 attcactatc tagaaaagca gccagaacaa atggctcagt tgatgcaatc caatcatccg
     1081 ctagaaatct taacgttgtt atctagttat gaataagtag cgattaaaaa actgccttta
     1141 tatttataat aattctatct atttatttgg aagtagaaaa ctaaatacaa aaaaagagga
     1201 tgtggccaaa gttgtggcac atccttttat tttgagtttc ttataatttt ttatagttgc
     1261 ttacgatgtc ttgactactg gtaaacggtg ttcaagagac aatttgtcgg caataaggca
     1321 caattttgta aaatatggaa agctattttt ttaacattgt ttcttagtgc ttgaaaaatc
     1381 attttgaata cttctttata cgatcgatgt acgttgattt aagtctaaaa cttttctttt
     1441 gattccttct ctgacttttc tgaattccgt taatatttct tcttcgctcc cgacagcttg
     1501 tgcaggatct tttaggtccc agtgaaggtg ttcgtgattg tttggaacgg cagggcactt
     1561 gtctttggca tcaccacaa
//
LOCUS       DACCSK010000218         1515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCSK010000218 DACCSK010000000
VERSION     DACCSK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.228"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IVP39_002795"
     CDS             287..694
                     /locus_tag="IVP39_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587830.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVP39_002796"
     CDS             708..1109
                     /locus_tag="IVP39_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587831.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVP39_002797"
     CDS             1111..1482
                     /locus_tag="IVP39_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587832.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DACCSK010000219         1499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCSK010000219 DACCSK010000000
VERSION     DACCSK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.229"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(143..1162)
                     /locus_tag="IVP39_002798"
     CDS             complement(143..1162)
                     /locus_tag="IVP39_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7587833.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1173..1379)
                     /locus_tag="IVP39_002799"
     CDS             complement(1173..1379)
                     /locus_tag="IVP39_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7587834.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          415 a          311 c          256 g          517 t
ORIGIN      
        1 tggcaacaaa cctgttatcg catagcttgg agtgtgacta ttatgggtga tagtgaagat
       61 atgcgataac atcactattt tatcgaatga tttttatagt tgtcaatata gttatgtaat
      121 gctcctcaac gaggagctat ttttatcgtt taggaatatt taaataccaa cgtttgtcat
      181 ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc attcgttgcc cgcatcatga
      241 catagacttt cttattaggg aagttacgca tgttaaaaga tacatgatag ccaacatttc
      301 cagaagtatt ataagcttga tttacatctg atctataaat tccatcagct cttactcgag
      361 ctaattcttt cccagtattg taatccataa tgaagatata ctcgtattta tagttagcaa
      421 tgtgccatcc agctacatgc aaatttgcat tttcgatttc tccaaactga tcaatgtggg
      481 cgtaattcgt tccatctgtc agtgtaggat tagctgcacc agctcgtgtt ggatcaatga
      541 caggcttgtt ttcagaagtt gttggatttt catcggtaaa tccatgagct aaatcatatg
      601 ctaatttttc tttacttacg cccatttcag aaagataacc gtaaggatct gtatgatcgc
      661 cccagatgtt ttgtgttacc cataaatgcg atttgattcc cggttggtta taaggagtgt
      721 ccaacgttaa tggaatacca tatttcattg ctgaatctct agccaattca acgtatgcct
      781 tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg taactcaatc tgcacaggac
      841 tgttggcatt agcatacgaa ccagcaccgt actgtacata accaggttga ccgacttgat
      901 aaacaattcc gccgtctccc acaatataag cagtataagc gctagtccat gaacgttgca
      961 tatactgcgc ttcattgcgt cctgttgctg tttcattagc cgtttcatgc agtaaaatat
     1021 actgattatt tgctacttgt gagctacctt catttgcgcc caaattaaat tcattgttga
     1081 tagtataggc aaatccgtta attggcaata aaaaaagagc cgttaacagg ctcatcgcag
     1141 taatagtaat tttctttttc atatctatcc tcctatttat ctgatgaaat tttcttatac
     1201 gttcgatttg atacacctaa aatactccct agaaatgctc caaaacctgt aataatcatc
     1261 accgttatat ctgtgtaccg ccagttaaca ccttttccga caagaccgac aaaagtcgct
     1321 aaagcgggga taactactat agcgatccat ttcaaaatat caaaggttct attgttcata
     1381 ctatccacct cccttcattg gttgggctac tttctttttc aatcgcttgt tttcttctat
     1441 caatatttct ctttctgaaa gcaaggcatc aatttgcccg tccagttgtt caattttat
//
LOCUS       DACCSK010000220         1497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCSK010000220 DACCSK010000000
VERSION     DACCSK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.230"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1..308)
                     /locus_tag="IVP39_002800"
                     /pseudo
     CDS             complement(1..308)
                     /locus_tag="IVP39_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(562..1116)
                     /locus_tag="IVP39_002801"
     CDS             complement(562..1116)
                     /locus_tag="IVP39_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7587835.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          485 a          257 c          155 g          600 t
ORIGIN      
        1 caaacctata taatcatgaa tgctggcaaa aattttttta agtcaagttc taaatcttct
       61 tcgaaaacac atttaatttc tcctactgat aattgatact ctatattaca tgacttaatt
      121 agttttttag atcccccctc cagataggca tatgtttcaa taattatgtt ctttttattg
      181 cttggcaaat ttccgttatt tattgcataa attaaaaatg actgtctatt tagcccataa
      241 tctaatacaa cttcttcata ctcatatttc tttaaatatt taatatgaaa atatgctgaa
      301 ttaatcaaaa ctcgagtgtt actgttgtta tttattgttg ttctattgtg tataaaattg
      361 tataggttgt catattttct tggtttatta aaatcattct ctatataata atcattatca
      421 actacaacat tttgttctat ctctggtatt ctattgtaaa tgttactaaa cgttttaaat
      481 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
      541 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      601 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      661 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      721 taatgtatgt gttcctatat catctcttcc taatttttct gctatatctg catatatctg
      781 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      841 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      901 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      961 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
     1021 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
     1081 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
     1141 tctgagttca tgattatata tatattttac cataatcatg aacttatata tatactctgt
     1201 taaaagtgaa gaaaatcaag gggtttagtt tatgatttta tatcatttca gtaactcaga
     1261 atattccgac ttttttgtac aaaaaaatta accttactta tatgaagatt ctaagctctt
     1321 atttaaattt tagacgggca gttatccgta gaagttctta gaatagctaa tttacataat
     1381 taaattatat gaaatttaaa tttgaacaat atactaagaa ctccccttac tataaattga
     1441 aataaattag catatgaata accgtgctac caagcttttc attaataaaa tattttt
//
LOCUS       DACCSK010000221         1496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCSK010000221 DACCSK010000000
VERSION     DACCSK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.231"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            153..1313
                     /locus_tag="IVP39_002802"
     CDS             153..1313
                     /locus_tag="IVP39_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7587836.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1329..>1496
                     /locus_tag="IVP39_002803"
     CDS             1329..>1496
                     /locus_tag="IVP39_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7587837.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAE"
BASE COUNT          507 a          243 c          305 g          441 t
ORIGIN      
        1 aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg caggacagct
       61 ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
      121 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
      181 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
      241 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
      301 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaacc
      361 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
      421 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
      481 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
      541 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
      601 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
      661 gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt aaaaatatac
      721 aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa gacttagtta
      781 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
      841 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
      901 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
      961 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
     1021 ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa cataagaaaa
     1081 aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat gggaaagtca
     1141 agctggatca attgcctggt ggaacttatc gaatcgtttc agctgatgat cctaaagttg
     1201 ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt gaggggcgaa
     1261 aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa taagtaagga
     1321 gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa tctgttctat
     1381 cgatttggct gcatgctaac ttggattctc atgaattcat tccatcaact tattgggaga
     1441 atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta gcagaa
//
LOCUS       DACCSK010000222         1485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCSK010000222 DACCSK010000000
VERSION     DACCSK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.232"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..995)
                     /locus_tag="IVP39_002804"
     CDS             complement(<1..995)
                     /locus_tag="IVP39_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7587838.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(1013..>1485)
                     /gene="srlA"
                     /locus_tag="IVP39_002805"
     CDS             complement(1013..>1485)
                     /gene="srlA"
                     /locus_tag="IVP39_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7587839.1"
                     /translation="IVPVVLMLMVAMNTLIAFLGEDRVTKVAKLSAKNPLTRYLVLPF
                     LSAFMLGNPMSFTMARFLPEYYKPSYYAAQAQFCHTSNGVFPHINPGELFVWMGIAQG
                     IETLGLNSMELAIRYLLVGLLMNFIGGWITDFTTGFVCRQQGIVLSKKVELSVD"
BASE COUNT          436 a          338 c          252 g          459 t
ORIGIN      
        1 cagctatata atccaaaact tgctacccaa gcaatgataa ctcgtgggat gccgttaaga
       61 aatcttgaat aaagaactga tggtacacct acttctactg tctcagcttt tgcttcttcc
      121 aaacctagac cgactggaat aaaatcacat gcattttgag tattgatagc aaatagtgcc
      181 ggtaatgcta attggggagg aatatttcct ttcccaatct ctacaccgat caatgttcca
      241 atcacttggc taataactgc ccctggacca agcaaaggag ataaaaatgg cagtgaacaa
      301 ataaagccaa tgattgttaa tccaactgca tttcctgcta aaggtgccat aatgttagca
      361 aatacattac cgatcccaga accttgtatc acaccaatca aaagggaaac gaaagccata
      421 aaagggatca ctgtccggat catcgtatct actgcttctc ttgctgattg attgaatgta
      481 gcaactactt ttcccgcgcc cattccaatt tttgctatcc agttttgctg ctctgctttt
      541 tgctcggtta tctttttaga agtatcatat ttgtactttt tatctggaga tgcagtattt
      601 actgtttctt tgttatcatc agtttccatt agtggctgta tttgttctaa ccctactgct
      661 gaaacataaa tatcttcagt gatatattgt gctaatgggc cactttttcc tgtgggaacg
      721 atattgatgg ttggtatccc ttttttagga taaattccac atctaagagt ccctccacag
      781 tcaacgattg caagcgcgat ttcactatca ggtattgatg ttttgaaccc attgatagac
      841 tccatacctg tcagttcttc tattttatct acgatcattg gcctctcacc tccacctgtg
      901 acatagatga acttgtgttt atcttttgtc ggcctaataa tcaaaggacc tccatacccg
      961 ccgtttcctc ttacgatctt aatatcacca tacatttaaa actctccttt ctctaatcaa
     1021 ctgaaagctc tacttttttg ctaagaacga ttccttgctg tctgcataca aatccagtag
     1081 taaaatcagt tatccaacca ccaataaagt tcatcagcag tcccacaagc aagtatcgga
     1141 tagccagctc catcgaattt agccctaagg tttcgatccc ttgagcaatc cccatccata
     1201 caaacaattc tccaggatta atatggggga atacaccatt gctcgtatga caaaattgag
     1261 cttgagcagc ataatagctt ggtttgtagt attctggaag gaaacgagcc attgtaaatg
     1321 acataggatt tcccaacata aaagcagata agaacggtaa cacaaggtaa cgagtaagcg
     1381 gatttttcgc tgataatttg gctactttcg ttactcggtc ttcacctaaa aaagcaatca
     1441 gcgtattcat tgcaaccatc agcatcaaga caactggtac gattc
//
LOCUS       DACCSK010000223         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCSK010000223 DACCSK010000000
VERSION     DACCSK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.233"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            76..1416
                     /locus_tag="IVP39_002806"
     CDS             76..1416
                     /locus_tag="IVP39_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7587840.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          462 a          322 c          284 g          388 t
ORIGIN      
        1 ctaataaacg caaacagaca ccttgaaatc ctttagaata aagtcgacga aaaccattca
       61 aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga tattctagac
      121 ctaaatctta cctttcatga agatgccttt cgcaaagagc gcattaatga cgaaacatgt
      181 tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg ccactatcaa
      241 aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt gaatgatgtc
      301 agtaactaca agaccattgt gcggattaac aaacagcgct tcaaatgtaa acactgtgga
      361 aagacttttt tagcagaaga ttcagttagc gatcgtcgct gctccattgc ccgcagagtc
      421 aaacaagcca ttcttgagct attaagtgag cctatttcta tgtctttgat tgcacgtatg
      481 aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc taaaaccgtt
      541 tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc ggtgaaaaac
      601 gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact gattgatatt
      661 gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc tagaaaagtc
      721 cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac attggtgaaa
      781 gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca acacatcggt
      841 cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg ggatcgtcgg
      901 tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga attgaatggt
      961 cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcgga gatagtcgat
     1021 cggctattat attatgatga ctccttaaag cgaggctacg aagtctacca ggtttttctt
     1081 tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga agactacaag
     1141 gagttgccag agcactatca accagtgttc acgaccttca agaaatatcg aacagaaatc
     1201 aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa caaccatatc
     1261 aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactaccg agaacgaatc
     1321 tttttatatc gaggaaaata tttcaagaaa actaagaaca ctacccaact gactaaagcc
     1381 agaacaacaa cacggctaga caagatagct gtctagccgt gtgagtatct cgattattct
     1441 attgaatttc ttcacc
//
LOCUS       DACCSK010000224         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCSK010000224 DACCSK010000000
VERSION     DACCSK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.234"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            109..1041
                     /locus_tag="IVP39_002807"
     CDS             109..1041
                     /locus_tag="IVP39_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP7587841.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1456
                     /locus_tag="IVP39_002808"
     CDS             1113..>1456
                     /locus_tag="IVP39_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587842.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          228 g          419 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaaga
//
LOCUS       DACCSK010000225         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCSK010000225 DACCSK010000000
VERSION     DACCSK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.235"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(136..804)
                     /locus_tag="IVP39_002809"
     CDS             complement(136..804)
                     /locus_tag="IVP39_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7587843.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            938..1225
                     /locus_tag="IVP39_002810"
     CDS             938..1225
                     /locus_tag="IVP39_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587844.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          522 a          227 c          228 g          416 t
ORIGIN      
        1 aaaaaaacaa actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag
       61 attttactga ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt
      121 agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc
      181 tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt
      241 attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc
      301 caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat
      361 cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc
      421 gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt
      481 gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa
      541 aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa
      601 tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa
      661 aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat
      721 attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc
      781 tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact
      841 tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg
      901 gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac
      961 aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca
     1021 aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt
     1081 tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg
     1141 tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga
     1201 gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa
     1261 aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga
     1321 acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta
     1381 ttaacgccga ttg
//
LOCUS       DACCSK010000226         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCSK010000226 DACCSK010000000
VERSION     DACCSK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.237"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            123..1292
                     /locus_tag="IVP39_002811"
     CDS             123..1292
                     /locus_tag="IVP39_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7587845.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          484 a          218 c          240 g          406 t
ORIGIN      
        1 ttttacactc tcataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacacataat tcttgacgct acctttaa
//
LOCUS       DACCSK010000227         1250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCSK010000227 DACCSK010000000
VERSION     DACCSK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.238"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(43..1083)
                     /locus_tag="IVP39_002812"
     CDS             complement(43..1083)
                     /locus_tag="IVP39_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7587846.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT          335 a          275 c          216 g          424 t
ORIGIN      
        1 aaaaggggat gtgacactgt cacactccct ttttaattta ttctatttct ttatatttat
       61 tttgtattcc gttacaaact cgcgtacttg gttaggttcc agagtgacat ctccaaaact
      121 ttcaagtcgc tccgctcctg gcagttcttg agtctcaaat gtgataccgc catgatttac
      181 taatttattc ccgcgcattt ccggtccatc ttctccaaaa tttgctgtaa aaatcacgat
      241 tcctgaagca tctgttttta cttggatcga tactttttca tctgggcttg tcaattccgc
      301 agcatttagt aaaccagatt gttttttcaa caaaaatgga tgatcgatgc cgcccactat
      361 cattgattgg cgaaaatcac tggcaaagac ctcacctaat tttttaggac gttggaagtc
      421 aaatgctgtg ccacttactg aaactacttc tcctgtaggc gttgtatctt tattgagcgt
      481 agcaaataat cggctattca accaaagtat atgttcgtcg attgactgtg ctggatcacc
      541 ggttaaattg aaatagacat gatttgtagg attgaatact gttttgcggt cactgatccc
      601 ttggaccgtc actcgccatg tatcatcgtt cgtcagcgta taccgtactt ctacatctaa
      661 agttccagga aatccgtttt ccatatctgg gctagtctga tagaaaatga ccgatgcttc
      721 gttttcacca ttgatgactg tataatgcca ctgcttggct tcaaagctag gtgcaccacc
      781 atgtaaacaa tgaccagtgt ccgggtctgt ttttacttcg aacgtttcat catctaattt
      841 gaatgtgcca ttcgtaatcc gtccagccat acgtccgatt gaagcaccga tataagggtc
      901 tttttctaga tattcttttg cttcatcaaa acctaagatc aactctcgtt tttcattatc
      961 gattattgtt tgaaaatgaa caacccgtgc cccaagatca ctgacagaca aagatgtccc
     1021 attttgattc gtgatcgtaa tcaagtgata gttttcgcca aaattttgtt cattgatttc
     1081 catgttcctt gcaccctctt ttctttcatt tgcactgatt ccagtataca tggaaaccgt
     1141 tttcttaaca gcttattccg tgagtaacga gaaaaaattt actatattct aataaaagac
     1201 cgtgaaaaaa aggttgtgac aaaaatattg tcacaacctc tttttccgaa
//
LOCUS       DACCSK010000228         1234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCSK010000228 DACCSK010000000
VERSION     DACCSK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.239"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            33..1234
                     /locus_tag="IVP39_002813"
                     /pseudo
     CDS             33..1234
                     /locus_tag="IVP39_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          514 a          162 c          216 g          342 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttt
//
LOCUS       DACCSK010000229         1217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCSK010000229 DACCSK010000000
VERSION     DACCSK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.240"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            115..327
                     /locus_tag="IVP39_002814"
     CDS             115..327
                     /locus_tag="IVP39_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587847.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            489..1007
                     /locus_tag="IVP39_002815"
     CDS             489..1007
                     /locus_tag="IVP39_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP7587848.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1095..>1217
                     /locus_tag="IVP39_002816"
     CDS             1095..>1217
                     /locus_tag="IVP39_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7587849.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGIN"
BASE COUNT          450 a          155 c          208 g          404 t
ORIGIN      
        1 gaaaagtgag tatctccatt cataaaataa tcaacatatt taaaaaatct aattagcttt
       61 tttagtcaat ctaaagttct ttattctatg atataatgtt actgtagaaa gtttgtggcg
      121 gtttggcaaa tccaattgaa ggagcgtggt gcctatgggc ttattagctt atcggacgta
      181 ctctgtagaa aggaaatgcc tgatttgttc tcattggatc aaatcattgc tattatcgga
      241 gcagcagcag tagttattac tgccgtagcg aaagctagcg attcgatttt agcttggtta
      301 aagttcaaaa aagaacaaaa aaaatagagc cgtctcaact tttggcgagg tagacgagct
      361 ctaaagcttt atttacatgc catccgcttt gaacggacta cactgggcat gtgacagcat
      421 gccctttcat ttatattata tctcattctt ttatataatg aaagtaaata aatcatggag
      481 gataaaatat ggataaattt aatagattaa cattaataaa tcagtttgaa attttaaaag
      541 ctttaaatcc taacgaagaa aaatactatg ctgaaaaaat agaaatacta acagaaggtt
      601 acgaatatca ctatagtgaa atatttgaaa atatttctga tccattgcca gaagaagatt
      661 cgcggtttgt tctagacata ttaaatatgt atagagatat tacttttagt aaaaataaat
      721 taaaagatat aaattcaatt gataattatt atatacaatt taaaggtttt gactttaata
      781 gctctaccga atttaagtta gcactttatg cacaattttt tattgaaaaa ttaaataggt
      841 ttcaagaaat agttgaagat agtgatttta acacttttaa tagccataaa ccgatgagag
      901 gtacttatat aaaatttctc gaaaattata atgatttaaa atctaccaat aattatcaat
      961 ttggaaactt gtcttatgag atgataagta aagttctaag tttgtgatga taaatgaaat
     1021 ggttctgttg caaagtttta aatctactat caaataaggt agaataatag aaaaagatag
     1081 caggaggaat gacgatgaat cattttaaag gaaagcaatt tcagcaggat gtgattattg
     1141 tagccgtggg ctactatctt cgttataacc ttagctatcg tgaagttcaa gaaatcttat
     1201 atgatcgtgg cattaac
//
LOCUS       DACCSK010000230         1192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCSK010000230 DACCSK010000000
VERSION     DACCSK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.241"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IVP39_002817"
     CDS             <1..1076
                     /locus_tag="IVP39_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7587850.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DACCSK010000231         1191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCSK010000231 DACCSK010000000
VERSION     DACCSK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.242"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..65)
                     /locus_tag="IVP39_002818"
     CDS             complement(<1..65)
                     /locus_tag="IVP39_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HAP7587851.1"
                     /translation="MANNRRLEETFNDGWLKILTQT"
     gene            complement(55..366)
                     /locus_tag="IVP39_002819"
     CDS             complement(55..366)
                     /locus_tag="IVP39_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587852.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(363..1169)
                     /locus_tag="IVP39_002820"
     CDS             complement(363..1169)
                     /locus_tag="IVP39_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587853.1"
                     /translation="MKKLGIKDIVLLFSRHDDQIPIFFEKEYGVEVHVYDDLRDDKEY
                     IPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNKRINLRKLPSKDDVWYCSDCCSYL
                     SLDYIRSCENGENILKDMANIVNVTVESLETINTNSGGAQWVINRPKANYWKKVYLDS
                     NRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGPKVHEELDFCFATDPIEKVKEVKI
                     LHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKKKCSYAYVKAIKAVVR"
BASE COUNT          361 a          250 c          168 g          412 t
ORIGIN      
        1 gtttgcgtca aaatctttaa ccaaccatcg ttgaatgttt cttcgagtct acgattattc
       61 gccattcaca tcacctgcaa tatattcttg tagcgcataa tgttgaatga aacctaataa
      121 ctcactagcg aaattttgtt caaactcatc taaagcacga ttccagtcgt atctacatct
      181 ttcgattagc aatccgtatt ctaagctttc aggagaaaaa gaaagtgttg tactcacttt
      241 actttgaaga taaacagcat ttttagctat catcttttta attgactcat cttcttcgtt
      301 ccaggtaacg taaatattat ccttcacagc tattagcaat tcttcagtca cttgttcagg
      361 cgtcatctaa ccaccgcctt aattgcttta acatatgcgt aagagcattt tttcttgttt
      421 acaaatgata aatcttcatc aaaaggcgta gaagtcacgt atctcccttt gaaaaataaa
      481 tcttcatcgt ttgttgttac tccagcattg tgtaagattt ttacttcttt aactttttct
      541 attggatcag tagcaaaaca aaagtctaat tcctcgtgaa ctttaggacc aatattgaaa
      601 tacatcatgt tccaaagctg tgcccacatc tcggctgtcc agatttgtat atttgttttt
      661 tgccctctaa ggtagcgata tagccgatta gaatccagat aaaccttttt ccaataattc
      721 gctttagggc ggttaataac ccactgtgcg cctcctgaat tagtgtttat agtttccaaa
      781 gattctactg taacatttac aatgtttgcc atatctttta gaatattttc tccgttttca
      841 cagcttctaa tataatcaag acttagataa ctacagcagt cgctacaata ccaaacatca
      901 tctttagaag gcaatttgcg caaattaatt cttttattga aaatgacatc cgaatcgata
      961 tagaaatatc ggtcgtcctc acgcgaatga tcttcttcta aatatttcca ccataaatat
     1021 ggtttaatcg aaggaatata ctctttgtcg tcccgcagat catcgtacac atgaacttca
     1081 acaccatatt ccttctcaaa aaaaatagga atctgatcat cgtgtctgct gaaaagcaat
     1141 acgatatctt tgattcctag tttcttcaga ttagttaaac aaacttcaag c
//
LOCUS       DACCSK010000232         1166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCSK010000232 DACCSK010000000
VERSION     DACCSK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.243"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            82..>1166
                     /locus_tag="IVP39_002821"
     CDS             82..>1166
                     /locus_tag="IVP39_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7587854.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPET"
BASE COUNT          387 a          226 c          224 g          329 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaac
//
LOCUS       DACCSK010000233         1165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCSK010000233 DACCSK010000000
VERSION     DACCSK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.244"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            54..1102
                     /locus_tag="IVP39_002822"
                     /pseudo
     CDS             54..1102
                     /locus_tag="IVP39_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          486 a          121 c          232 g          326 t
ORIGIN      
        1 tttggaaaaa taaaaaatta tatttcatca acgtttcata aaaggtgtgt agaatgttat
       61 tgaaagtagc tataaaatgc tattgtgtaa aaaataaagg aggaataatt tatgaaaaaa
      121 tagtaccaag aggagcatgc ctattacttg cattatgttt gagcttaagc gcagttgggt
      181 gtcaaaaaag taatgaaaac aataaaataa aagaaggaca atccataagt tctgaagagg
      241 aaggcatgtc aattaatgca aatggcaaaa atgaaacttt taagccatca gattatacat
      301 tggaggcaaa gaaagaatat gtttatgaat atttagggct taaatttaaa ctctcagaca
      361 aatttagaaa ttatatagct gataaaaaga tagcgatgtt agatgatcaa agtcctatag
      421 ataaagaact aaagtatgct attttgacat ttgagaaaat gacagaagaa caaaagaatg
      481 cagttataga aaagatgggc gatggatata aaaattggca aaatgagctt gaaagaattg
      541 ggactattgg tatatttgaa aaaaatacat cagaagaaaa aaatctaaaa ttataaaatg
      601 tgatactcat actaaaatag gagtttcaag tgatggaaaa tacgattgct atttaagcac
      661 aaatagcgga gctgaaagta atcttcttga tgaattaaaa agaactgaga ttcaaattat
      721 agataaaaaa gaacgtccta aaaatggttt tgtgctatca gaaaaaactg atttagaaaa
      781 tacagaagca tttaacaaag aatcggttaa agatttacgt aaactaccaa caaaggacat
      841 aaatggcaag gattttacaa gtaaagattt tgagaagtat gatcttacta tggtaaatgt
      901 atttgcaact tggtgtacag cttgtgtaaa ggaaattcct gatttggttg aggttcagaa
      961 tgaaatgaaa agcaagggtg taaatatcgt tggagttgtt acagatcctg ttgatgataa
     1021 cggtgaaaac aaggaagcaa ttgaaaagtc aaagttaata catgaaaaga caaaggcttc
     1081 atatcctttc ttaatgccag atggaatggc aacacttttt ttgacaaaaa ttataaggtg
     1141 ttgtgaatgc tgcggtgtta tggct
//
LOCUS       DACCSK010000234         1089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCSK010000234 DACCSK010000000
VERSION     DACCSK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.245"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IVP39_002823"
     CDS             414..947
                     /locus_tag="IVP39_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7587855.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          350 a          203 c          200 g          336 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgttta
//
LOCUS       DACCSK010000235         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCSK010000235 DACCSK010000000
VERSION     DACCSK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.246"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            2..>1073
                     /locus_tag="IVP39_002824"
     rRNA            2..>1073
                     /locus_tag="IVP39_002824"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACCSK010000236          985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCSK010000236 DACCSK010000000
VERSION     DACCSK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.247"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..623
                     /locus_tag="IVP39_002825"
     CDS             <1..623
                     /locus_tag="IVP39_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587856.1"
                     /translation="REDDRYFYIDSDVIFNKRINLRKLPSKDDVWYCSDCCSYLSLDY
                     IRSCENGENILKDMANIVNVTVESLETINTNSGGARWIINLPKANYWKKVYLDSNRLY
                     RYLRGQKTNVQIWTAEMWAQLWNMMYFNIGPKVHEELNFCFATDPIEKFKEVKILHNA
                     GVTTNDEDLFFKGRYVTSTPFDEDLSFVNKKKCSYAYAKAIKAVVR"
     gene            620..931
                     /locus_tag="IVP39_002826"
     CDS             620..931
                     /locus_tag="IVP39_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587857.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPDSLEYGLLIERCRYDWNRALGEFEQNFASELLGFIQHYALQEYIAGDGNG
                     E"
     gene            921..>985
                     /locus_tag="IVP39_002827"
     CDS             921..>985
                     /locus_tag="IVP39_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HAP7587858.1"
                     /translation="MANNRRLEETFNDGWLKILTQT"
BASE COUNT          343 a          140 c          206 g          296 t
ORIGIN      
        1 cgcgtgagga cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta
       61 atttgcgcaa attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc
      121 taagtcttga ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa
      181 atattgtaaa tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac
      241 ggtggattat taaccttcct aaagcgaatt attggaaaaa ggtttatcta gattctaatc
      301 ggctatatcg ctaccttaga gggcaaaaaa caaatgtaca gatttggaca gccgagatgt
      361 gggcacagct ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattaa
      421 acttttgttt tgctactgat ccaatagaaa aatttaaaga agtaaaaatc ttgcataacg
      481 ctggagtaac aacaaatgat gaagatttat ttttcaaagg gagatacgtg acttccacgc
      541 cttttgatga agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgctaaag
      601 caattaaggc ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt
      661 gaaggataat atttacgtta cctggaacga agaagatgaa tcaattaaaa agatgatagc
      721 taaaaatgct gtttatttac aaagtaaagt gagtacaact ctttcttttt ctcctgatag
      781 cttagaatac ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttaggtga
      841 gtttgaacaa aatttcgcta gcgagttatt aggtttcatt caacattatg cgctacaaga
      901 atatattgca ggtgatggga atggcgaata atcgtagact cgaagaaacg ttcaacgatg
      961 gttggttaaa gattttaacg caaac
//
LOCUS       DACCSK010000237          983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCSK010000237 DACCSK010000000
VERSION     DACCSK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.248"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            129..983
                     /locus_tag="IVP39_002828"
     CDS             129..983
                     /locus_tag="IVP39_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7587859.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
BASE COUNT          377 a          143 c          164 g          299 t
ORIGIN      
        1 aagagggctt ttatatatta atcacaaatc acttatcaca aatcacaagt gattaatcac
       61 aaatcacttg tgatttgtga ttcttaatga tacaatatta ctatacaaaa aaagaatggg
      121 gcgtagttat ggagaaggaa aaactaaaaa tacttgaaga attaagacgt attttaaaca
      181 ataaaaatga agcaattatt atcttgaata attactttaa aggtggtgtt ggaaagtcca
      241 aattatcgac tatgtttgct tacttgacag acaaattgaa tttaaaagtt ttaatgatcg
      301 ataaggactt acaggcaaca ttgacaaaag acttagcaaa aacatttaag gtagaattgc
      361 cacgtgtcaa tttttatgaa ggactgaaaa atggaaactt ggcttcttct attattcatt
      421 tgactgataa tttagacttg attcctggca cgtttgattt gatgttactg ccaaaattaa
      481 ctcgctcatg gacgtttgaa aacgaaagta gattgcttgc tactctttta gcacctttaa
      541 aaagtgacta tgatctcatt attattgata ctgtaccaac gccaagcgtt tatacaaata
      601 atgcaatcgt ggcaagtgat tacgttatga tccctttaca agcagaagaa gaaagtacaa
      661 acaacattca aaactatatt tcctatttga ttgatttaca agaacaattt aaccctggac
      721 tagatatgat cggttttgtt ccttatttag ttgatacgga cagcgcaacg ataaaatcaa
      781 acctggaaga actgtacaag caacataaag aagataactt ggttttccaa aatattatca
      841 agcgaagtaa taaagtaagt acctggtcta aaaacggaat tacagaacac aaaggctatg
      901 acaaaaaagt tttatccatg tatgagaacg tattttttga aatgcttgag cgaattattc
      961 aattagaaaa cgaaaaagaa tag
//
LOCUS       DACCSK010000238          952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCSK010000238 DACCSK010000000
VERSION     DACCSK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.249"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IVP39_002829"
     CDS             <1..782
                     /locus_tag="IVP39_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7587860.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(818..>952)
                     /locus_tag="IVP39_002830"
     CDS             complement(818..>952)
                     /locus_tag="IVP39_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7587861.1"
                     /translation="REKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK
                     "
BASE COUNT          386 a          135 c          121 g          310 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc tttttgaact gaaccccaaa agttgaacta tttaatggac tgtttccgat
      841 attctactgg agatagtcca tttaacttta gttttatcct tttgttatta taccacctaa
      901 tatactcatt taatttactt tgaaatatct caattgatcg gaatttttct cg
//
LOCUS       DACCSK010000239          948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCSK010000239 DACCSK010000000
VERSION     DACCSK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.250"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..>948)
                     /locus_tag="IVP39_002831"
     rRNA            complement(<1..>948)
                     /locus_tag="IVP39_002831"
                     /product="16S ribosomal RNA"
BASE COUNT          202 a          299 c          211 g          236 t
ORIGIN      
        1 agtgcccaac tgaatgatgg caactaacaa taagggttgc gctcgttgcg ggacttaacc
       61 caacatctca cgacacgagc tgacgacaac catgcaccac ctgtcacttt gcccccgaag
      121 gggaagctct atctctagag tggtcaaagg atgtcaagac ctggtaaggt tcttcgcgtt
      181 gcttcgaatt aaaccacatg ctccaccgct tgtgcgggcc cccgtcaatt cctttgagtt
      241 tcaaccttgc ggtcgtactc cccaggcgga gtgcttaatg cgttagctgc agcactgaag
      301 ggcggaaacc ctccaacact tagcactcat cgtttacggc gtggactacc agggtatcta
      361 atcctgtttg ctccccacgc tttcgagcct cagcgtcagt tacagaccag agagccgcct
      421 tcgccactgg tgttcctcca tatatctacg catttcaccg ctacacatgg aattccactc
      481 tcctcttctg cactcaagtc tcccagtttc caatgaccct ccccggttga gccgggggct
      541 ttcacatcag acttaagaaa ccgcctgcgc tcgctttacg cccaataaat ccggacaacg
      601 cttgccacct acgtattacc gcggctgctg gcacgtagtt agccgtggct ttctggttag
      661 ataccgtcaa gggatgaaca gttactctca tccttgttct tctctaacaa cagagtttta
      721 cgatccgaaa accttcttca ctcacgcggc gttgctcggt cagactttcg tccattgccg
      781 aagattccct actgctgcct cccgtaggag tttgggccgt gtctcagtcc caatgtggcc
      841 gatcaccctc tcaggtcggc tatgcatcgt ggccttggtg agccgttacc tcaccaacta
      901 gctaatgcac cgcgggtcca tccatcagcg acacccgaaa gcgccttt
//
LOCUS       DACCSK010000240          934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCSK010000240 DACCSK010000000
VERSION     DACCSK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.251"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..83
                     /locus_tag="IVP39_002832"
     CDS             <1..83
                     /locus_tag="IVP39_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7587862.1"
                     /translation="RIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            262..>934
                     /locus_tag="IVP39_002833"
     CDS             262..>934
                     /locus_tag="IVP39_002833"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7587863.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMN"
BASE COUNT          281 a          179 c          174 g          300 t
ORIGIN      
        1 aacgaattgc gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac
       61 aaggtctgat ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga
      121 ctaagtttta ttggctctat aatttctagg actgatggaa attttctatc tgtcctagtt
      181 tttttgtaaa aaaacaaaaa gacatacaaa atttccggta gaatagagtc tcctacaaca
      241 tcattctggg ggaattcttg tatgtccatg aatcatttta tcaaacaact ctttactatt
      301 aaagactcaa atatccaaat tcaatccttt gaagaaactt tttataaaag tagaaaaagt
      361 atcgttattg agggaactct gatccgtacc tacagacgtt gtccgtgttg taaagattct
      421 acaaagcaaa tcgttaaaaa tggcaagaag atctctatga ttctcctgaa tcgtagcggt
      481 aataaacgga cctatcttcg gttgaagaaa cagcgatacc tctgccgagc atgtaaaaaa
      541 tattttaccg ctcggacgta tctcgttacc ccattctgct ttatttctaa gcagattcat
      601 tacaagatct tagaagaatt gacagagcgt caatctataa aagcaattgg taaacattgc
      661 gatgtttctg tgacaactgt tcagcggaca ttgtccgcct tgtcatcaga tatccgtcag
      721 aatcttgagt atttacctcg gtgtctatta ttcgatgagt ttcgttctct ttctacatgg
      781 cacggtaaat tttctttttc ttgtatggac ggtgagaccg ggaaactgtt tgagatctta
      841 cctagccgcc gtaaaaaaga tctcgttgct tttttcatgc gtttttctct caaagcgagg
      901 ttgggtgtac attacatcgt aacggatatg aacg
//
LOCUS       DACCSK010000241          900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCSK010000241 DACCSK010000000
VERSION     DACCSK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.253"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(238..651)
                     /locus_tag="IVP39_002834"
     CDS             complement(238..651)
                     /locus_tag="IVP39_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP7587864.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKAQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          310 a          117 c          107 g          366 t
ORIGIN      
        1 ctataattga tttatacgac tcatttttga gtcgtcagac tgcaaacaac actctaaatt
       61 taggagtttg tctacagtct gaataaattt taaatttatt gtccataatg ttattatgaa
      121 tttatagcat taagaaaaga gataagactt tacatttttt taggaatgta aagtcttatc
      181 tcttttctta attgtacaat tagtattatc tgcactactt tctaattaaa taaattttta
      241 gttattaact tgattaaaat cagagataat atcatttaat tcagtttgaa gggttgtaac
      301 gattaaatgg aaattttctt catctgtttt attatatggg gatgttttcg ttatatataa
      361 caacttttct ttttcaattt tcattaataa ctctgtcgaa gtctgtgctt tatcttgata
      421 attaaatgca gacataccac tttgtataat tgtaattatt gctgaagcaa ttgaaacaat
      481 caatagtaaa gacctgtgtt cggaagcctg atcaattaat attgggatac tagctgataa
      541 gactattttt ataatatttc cgataccaat tactctatta taaaatcgaa tttctttttt
      601 taatctatcg attgtagaat caatagattc gataaacgct ttttgatcca tcattttctc
      661 tccaatcatt ttattaaaat cattaacaaa aatattataa taggatttaa gtaagttcta
      721 actaatcaca cataaaaata aaaaaacatt tgtattttat caaagtttta ttatacttgt
      781 caatttgtgt acatcctaaa atataagcta tttgtttact tctgtagaat atatactttg
      841 ttgttagata agttgcacat gtttatagaa tctaaataat aaatttttat gtaccgcccc
//
LOCUS       DACCSK010000242          876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCSK010000242 DACCSK010000000
VERSION     DACCSK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.254"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..141
                     /locus_tag="IVP39_002835"
     CDS             <1..141
                     /locus_tag="IVP39_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP7587865.1"
                     /translation="RSNKVSTWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENE
                     KE"
     gene            233..448
                     /locus_tag="IVP39_002836"
     CDS             233..448
                     /locus_tag="IVP39_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7587866.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            465..737
                     /locus_tag="IVP39_002837"
     CDS             465..737
                     /locus_tag="IVP39_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7587867.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
BASE COUNT          360 a          111 c          170 g          235 t
ORIGIN      
        1 cgaagtaata aagtaagtac ctggtctaaa aacggcatta cagagcataa aggctatgac
       61 aaaaaagttt tatccatgta taagaacgta ttttttgaaa tgcttgagcg aattattcaa
      121 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
      181 atttgtgatt gttgatgata aaataagagt aagaagaaat agaaagaagt gagtgattgt
      241 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
      301 tataatggga gataagacgg ttcgtgttcg tgctgacttg caccatatca taaaaatcga
      361 aacagcaaag aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
      421 acggaaatat ttacctgaca aactttaaaa ggagtgaaaa tgaaatggca gttacgtatg
      481 aaaaaacatt tgaaatagag atcattaacg aattatcggc aagcgtttat aatcgagtat
      541 taaactatgt tttgaaccat gaattaaata aaaatgactc tcaattattg gaagtcaatt
      601 tattaaacca attaaagctt gcaaaacgtg taaatctttt tgattattct ttagaagaat
      661 tacaagccgt tcatgagtat tggcggtcaa tgaatcgtta ctcaaaacaa gttttgaata
      721 aagagaaagt ggcttaatat ggcaaatata gtcaatttta ctgacaaaca atttgagaat
      781 cgcttaaatg ataatttaga agaattgatt caaggaaaaa aagcggttga atcgccaacc
      841 gcttttttac ttggtgggca accagggtca gggaaa
//
LOCUS       DACCSK010000243          865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCSK010000243 DACCSK010000000
VERSION     DACCSK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.255"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..231
                     /locus_tag="IVP39_002838"
     CDS             <1..231
                     /locus_tag="IVP39_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587868.1"
                     /translation="NLTTVSYNLAESGETENASVQNIVTKEAAPKPINKVHNGYRPAN
                     VAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            292..834
                     /locus_tag="IVP39_002839"
     CDS             292..834
                     /locus_tag="IVP39_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587869.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
BASE COUNT          359 a          121 c          156 g          229 t
ORIGIN      
        1 aatctgacaa ctgtttctta caatttagct gaaagtggtg aaacagagaa tgcgtccgtt
       61 caaaatattg tgacaaaaga agctgcacct aagccaatca ataaagttca taatggatac
      121 agaccagcaa acgtagcatt tactgcaccg acaaaaataa atgtgacaag agaaactaaa
      181 aacaggaaca atgaattgaa agaacaacta ttgaaggata tcaaaccata aaaggcctat
      241 ttaagggcct ttttctttat aaaacaagtt taaaatggaa aggagaaaat tgtggaggat
      301 attttaatag ctacgtttat aactgcactg ggagcatttt taacgatatt tgtggtgact
      361 attaaaggat ttaaagatac ggatagttta caaaaagatc atcagtctat tttagatggt
      421 caaaaaacac ataccgaaag cattattgta cacctttcag aaggattcac ttatttgaaa
      481 gatgctatgc ccacattcac agtaaaatta acggaaatat ttcattctgt tgagcaaata
      541 tcgtcagaat tgaaagagca aagacaacaa aaagatcgtc aacttggctc acttaatttt
      601 aggcaagaaa cgatacaaga atcattggag aatgtaaatc gattaaacga tgaaatgcaa
      661 cgagttcatt tggaaaatga agaattacgc gcacaaaatt atgatttaat tcaaaagaaa
      721 agaaatctgg aacaaaaaat tgttgaatta aaagaagaaa atcgtcaatt gaattatcaa
      781 ttacgacgag gaaaaaatcg ttctcgtgat ttaggaaatg aattagagtt gtaatattta
      841 gaaaaacgct gtgagaaatc acagt
//
LOCUS       DACCSK010000244          815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCSK010000244 DACCSK010000000
VERSION     DACCSK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.256"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..221
                     /locus_tag="IVP39_002840"
     CDS             <1..221
                     /locus_tag="IVP39_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7587870.1"
                     /translation="NTGLTFSIENGADEVAALELETKVMQDEFGKFYHEAIVAELEED
                     LTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            241..603
                     /locus_tag="IVP39_002841"
     CDS             241..603
                     /locus_tag="IVP39_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587871.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            606..788
                     /locus_tag="IVP39_002842"
     CDS             606..788
                     /locus_tag="IVP39_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587872.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
BASE COUNT          312 a          116 c          161 g          226 t
ORIGIN      
        1 cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct gcgttagaac
       61 tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca attgttgcag
      121 aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat gtgattaaaa
      181 aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg aggtagcgaa
      241 atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa tttagaacct
      301 aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga cgagtttgca
      361 aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc aaaggctgtg
      421 tatattgctt accgccaagc gaacatgact gattatattt cattcgatga atttatcgat
      481 aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat gttcaaacaa
      541 gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaaaaaa gcttcaaaag
      601 taaaaattcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg ttgaccgacg
      661 ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac attttgcaaa
      721 tggcttacaa caagagtgct tatgaaggat ggaaaaacta tgcaataaac caatcccaga
      781 aaaactaaag aaaggaggta aaaaatggct aaaaa
//
LOCUS       DACCSK010000245          789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCSK010000245 DACCSK010000000
VERSION     DACCSK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.259"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>789
                     /locus_tag="IVP39_002843"
     rRNA            <1..>789
                     /locus_tag="IVP39_002843"
                     /product="23S ribosomal RNA"
BASE COUNT          196 a          172 c          251 g          170 t
ORIGIN      
        1 catgtacagg ataggtagga gccgtagaaa tcggaacgct agtttcgatg gaggcgctgg
       61 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
      121 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      181 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      241 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      301 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      361 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      421 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      481 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      541 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      601 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      661 cccacctcaa gatgagattt cccatttctt taagaaagta agatccctga gagatgatca
      721 ggtagatagg tcaggagtgg aagtacagtg atgtatggag cggactgata ctaatcgatc
      781 gaggactta
//
LOCUS       DACCSK010000246          752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCSK010000246 DACCSK010000000
VERSION     DACCSK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.260"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            511..741
                     /locus_tag="IVP39_002844"
     CDS             511..741
                     /locus_tag="IVP39_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587873.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          275 a          122 c          117 g          238 t
ORIGIN      
        1 tgaatggata tttactttta atagataaaa tattataact attaaaaata actattttca
       61 ttataattac acacaaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
      121 ctaaacacgt ataaacagcg gaagcagaag caactccttc ggaaataagc tgaaattcac
      181 aaaaatttga aaagcaattt ccgtgaattt cttcttattt cttggagtta aacgcttctg
      241 ttccagcctc tttttatctg tctttcacta atgatcagtt gtagataagc aagttgcttt
      301 ttcttacatc ataccgccca tcaagacaaa tgtttttcac ttgtccacac tggacaaaaa
      361 tcccatcaaa tcaaggaaaa gcaacgttgg caatgtttga attttttagg ctagaacgga
      421 ctagtaagag ttttttgccc cttttttgcc cctcaaatat cgctttacaa caagaacgta
      481 agttcgtata atcgttttga ggtgattttt atgatggaag aatttattag aaaaaatatc
      541 agtgatgaat atgcagactt ttatgatcaa agcagtaaaa aagataaatt ccagatggat
      601 gtttcaatct tagctatatt agctttttcc gaaaataaac aacctgtaac tgcaaaaaaa
      661 gaaacagtat tctctgaagg caaaataaaa actcgatata tattagaggt agaaactaat
      721 tttaaaaaca gatcggagta atggttatgc tt
//
LOCUS       DACCSK010000247          749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCSK010000247 DACCSK010000000
VERSION     DACCSK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.261"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(295..555)
                     /locus_tag="IVP39_002845"
     CDS             complement(295..555)
                     /locus_tag="IVP39_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587874.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          296 a          112 c           82 g          259 t
ORIGIN      
        1 atttcttttc tttctagcca tcgtgtaatc acctcttgat cttattgtag aaaaagaaaa
       61 accgcgaagc aaccacctgc ctaggattaa tggttactta cacggtttat attacactct
      121 catcattaaa aaagtatctt tcgatattac taaacaatga aattatctat ttgaaattta
      181 aatctgataa tatattgagt gttccaatga gggctttgat cctattatcc atatcttttt
      241 cagttttaat attgatacac attataagaa aaagaaactt ttggttaatt tatttcatcc
      301 cagtgcaaaa tataaaaaag taaaatagac cataggcaat agattaacaa ttaatataat
      361 acaagcaaat attctattca ttgtatttct gctttcaaaa agttctaata ttgaaagtat
      421 aattccacaa atgcctataa taagtatggg aataagatta gaaaataaca ccccgagcca
      481 atctgtgaaa attactatat ttactaatat ccctattaac atacctatca tatttattaa
      541 atcaattttt ttcattagat atttctcctt tattaaatct gaagtactta tattaaaata
      601 attctaaact atttacctca aaaagtgcaa taaattattt ctgaacaata aaatgatagt
      661 caaaacaaaa aaatattgtt aatcgttcag agccatccga gagtgtaaaa tattttgtgt
      721 aaatgaaaaa atccatacaa aaaaggaag
//
LOCUS       DACCSK010000248          743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCSK010000248 DACCSK010000000
VERSION     DACCSK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.262"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(266..466)
                     /locus_tag="IVP39_002846"
     CDS             complement(266..466)
                     /locus_tag="IVP39_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7587875.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          266 a          118 c          126 g          233 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat att
//
LOCUS       DACCSK010000249          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCSK010000249 DACCSK010000000
VERSION     DACCSK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.263"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            4..>734
                     /locus_tag="IVP39_002847"
     CDS             4..>734
                     /locus_tag="IVP39_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587876.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPE"
BASE COUNT          322 a          116 c          161 g          135 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaa
//
LOCUS       DACCSK010000250          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCSK010000250 DACCSK010000000
VERSION     DACCSK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.264"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            97..627
                     /locus_tag="IVP39_002848"
     CDS             97..627
                     /locus_tag="IVP39_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587877.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(622..>731)
                     /locus_tag="IVP39_002849"
     CDS             complement(622..>731)
                     /locus_tag="IVP39_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587878.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          293 a           86 c          111 g          241 t
ORIGIN      
        1 ttctaacatc agttattatg ataattttga tgatgtcaat tatgatttac aggaaagata
       61 taatgattta atcgaagaat tagaggagag aggagaatga tttcagataa taaatgggaa
      121 ataaatctca aaagatttat ttcagaatat atatatacga ttgaaactaa atctagtttt
      181 tctcttaatg aagacagtga tcctctaaaa aaatacacta ttcaatgtaa gaaaaataaa
      241 gatgaaaaat tgttcgtaat aagaccagat cttatgaaga gtaataccca ctatataaaa
      301 gaaaaaccta aagattgtga ctacatcatc ataagtgaag caaataaaac agtatttctg
      361 atagaactta aaagctcagc tcagacttcc cctgcaaaag aaattagtga acaaattgat
      421 ggtggatata agtggttaca acatcttagt tttctaacaa aatatgattt tactgaattc
      481 agttataaaa ttattaaggt atgttgtaaa tttgaggcaa gacaagatcg taaaaatctt
      541 tttaaaccta atgaatatgg tatttataat atgaatggtc caaaattaga cttaagagag
      601 ttgtatagaa agtgtaacga tctataaatt caaatctttt ttaattctat atattgtttg
      661 tctagtaacg ttataattct ttgcaatctt tgaaatagag acgccctcat tcaagtcctt
      721 aacgatgttt t
//
LOCUS       DACCSK010000251          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCSK010000251 DACCSK010000000
VERSION     DACCSK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.265"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVP39_002850"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVP39_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7587879.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IVP39_002851"
     CDS             complement(481..675)
                     /locus_tag="IVP39_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587880.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACCSK010000252          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCSK010000252 DACCSK010000000
VERSION     DACCSK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.266"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            58..474
                     /locus_tag="IVP39_002852"
     CDS             58..474
                     /locus_tag="IVP39_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7587881.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT          223 a           99 c          173 g          200 t
ORIGIN      
        1 ctaacataat aaaacagaca agtttattat atcacataaa aggagcagtt tgacttgatg
       61 caattgttac gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag
      121 aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt
      181 attacggata tgccgaaggc accgaagcac ggcaataagg cagaggatgc attgattcag
      241 atgatggata tagaagcgga gagagacgcg attttagcgg ctttgatggc tcttagtctg
      301 attagtcgtc agatactcta ctatagcttc tgtgacgtaa ataagcactc taattatgaa
      361 ataggtcaat tgatacgagg atacggagag aagaatgtag agaagctgaa atctatcgcg
      421 ctgatcgaat ttgctgaagc atacaaaaaa ggcgtgttag ttcagtatcg ttgattttgt
      481 agggtttttg tagggatagt gtagggtttt tgagtgtttt aacgtgatat tatgatagtg
      541 tcgaaagatt aggaaacagg atcgacaaaa taaaatgtaa gggagggaat ctccctcatc
      601 gttttaaatt aagcttcgat aggcagcaac ggaaatatta agaataagga tgtgaatttc
      661 aactcctcct gattgttctt attatctatc atccg
//
LOCUS       DACCSK010000253          692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCSK010000253 DACCSK010000000
VERSION     DACCSK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.267"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            56..>692
                     /locus_tag="IVP39_002853"
     CDS             56..>692
                     /locus_tag="IVP39_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7587882.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFS"
BASE COUNT          204 a          137 c          133 g          218 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc at
//
LOCUS       DACCSK010000254          637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCSK010000254 DACCSK010000000
VERSION     DACCSK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.268"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>637
                     /locus_tag="IVP39_002854"
     CDS             <1..>637
                     /locus_tag="IVP39_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP7587883.1"
                     /translation="NRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRN
                     EKALMASMLEMYVSGVSTRKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLS
                     EKNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTF
                     FEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIP"
BASE COUNT          217 a          129 c          124 g          167 t
ORIGIN      
        1 aaaccgacaa agtcaacgaa atggctatta tgagcgcagc tttacgacac gtgtaggcac
       61 gctagaatta aaagtaccca gaacacgtga tggccatttt tcacccacag tgtttgaacg
      121 ttatcaacga aacgaaaaag ccctcatggc ttcaatgttg gaaatgtatg tatcaggcgt
      181 ttcaactcgt aaagtatcaa caattgtgga agaactttgt ggtaaatccg tctctaagtc
      241 cttcgtttct agcttaacag aacagctaga acctatggtt aacgagtggc agaatcgttt
      301 attatcagaa aaaaattatc cttacttaat gaccgatgta ctctatataa aagtacgaga
      361 agaaaatcga gtactctcaa aaagctgtca tatagcgatt ggaataacca aagatggcga
      421 ccgtgaaatt atcggcttca tgattcaaag tggcgaaagc gaagagacct ggacaacatt
      481 ttttgaatac ctaaaagaac gcggtttaca aggtacggaa ctcgttattt ctgatgcgca
      541 caaaggatta gtctctgcca ttagaaaatc cttcaccaac gtaagttggc aaagatgcca
      601 agttcacttc ctaagaaata tctttaccac cattcct
//
LOCUS       DACCSK010000255          606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCSK010000255 DACCSK010000000
VERSION     DACCSK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.269"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            166..372
                     /locus_tag="IVP39_002855"
     CDS             166..372
                     /locus_tag="IVP39_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587884.1"
                     /translation="MVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQ
                     ISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          261 a           76 c           68 g          201 t
ORIGIN      
        1 gaattatatg tttttaatag tttagatgtt gaaataccta tgaaacagat gaaaaatatt
       61 gacatacata tactgccaag tgagtcgtgc caaatagaat attcaatcga atatttttac
      121 aaaggtaaaa aaataaaaaa atgtgacaag tgttttgaat cagctgtggt aaagtttcct
      181 caatttaaac taaatccagc tgcctcgata caatttcaag gacatgtatt tgatgtttta
      241 gctaaagata acataaaaaa atacaaaaaa ggtaatatat tccatattga tatacaaata
      301 tcttctaatt tagaagatat aaaaaaagcc tttaaaatta aaacaattgg cactgctcct
      361 aattttaaat aacaaaaaaa ttactagata atctaaaaac aggatggcat tatgagaaca
      421 ataactaaac ttcctatatc acatattttg taattagtga aaagcaaatt gactactatc
      481 ataagttaat gactctcatc ttaatattta aaatatattc atatttttct attttatcta
      541 aatgcgattt tcatatctaa taatagaaaa ataaacaatt aacaataaaa aagtcttcat
      601 ttattg
//
LOCUS       DACCSK010000256          603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCSK010000256 DACCSK010000000
VERSION     DACCSK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.270"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..460
                     /locus_tag="IVP39_002856"
     CDS             <1..460
                     /locus_tag="IVP39_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7587885.1"
                     /translation="TMGGLMARLSKFNIEGVDDPIYRNVNPSDVVLIVNPTDYWSKVF
                     PAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDEYH
                     FVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
BASE COUNT          200 a          111 c          119 g          173 t
ORIGIN      
        1 aactatgggc ggtttgatgg cacgactatc aaaattcaat atcgaaggtg tagatgatcc
       61 gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa ctgattactg
      121 gtctaaagta ttcccagcta agactgtact aactgctaac ggtgaatacg tacaagtatt
      181 accagtacca gtttcagact tgcagtcaac tgctgtgcca gaaggaaaag cagttattgg
      241 ggtagcttca gattacttta tgggtgtagg atctacacta aaaattgaag cttcagatga
      301 ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa acggtcaacc
      361 taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta ctactacaac
      421 tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa tgaagtatat
      481 tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt taactaatct
      541 gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg atcagattcc
      601 tat
//
LOCUS       DACCSK010000257          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCSK010000257 DACCSK010000000
VERSION     DACCSK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.271"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>591
                     /locus_tag="IVP39_002857"
     CDS             <1..>591
                     /locus_tag="IVP39_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587886.1"
                     /translation="TLDNQYAAESGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNQPIY
                     LELAKLCEQVPEKEQKQSVKNEPVTKEQMQSKETKTKQKGYQKRTAEEIKQEVKSLSQ
                     QALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGI
                     YIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPED"
BASE COUNT          230 a          100 c          132 g          129 t
ORIGIN      
        1 acgttggata accaatacgc tgcagaatca ggttattata aattctattt tgaagaagtg
       61 aagaacggag aagtagtcga tcagatccga atggatctag gggatggcgt caacagtaat
      121 caacccatct atttagagtt agcaaaattg tgcgaacaag tacctgagaa ggaacaaaaa
      181 caatcggtca aaaatgaacc tgttacgaaa gaacaaatgc agtcgaaaga aacaaaaaca
      241 aaacaaaagg gctatcaaaa acgaacggca gaagagatta aacaagaagt taaatccctt
      301 tcgcaacagg cactggatgc ggtgaaaaag catacccatt ctcctgagga cgtcaaagaa
      361 cttttgaact ttatgtctcg ttttccagaa cgatccttta gaaatcaatt attgattgag
      421 gaacaatttc caggtgcaac ggcatgttta ggtcgtgccg cgttgaaaaa agaaggaatc
      481 tatatcaaaa aaggggaaca agggaacaag atctttgtca gaaaaacggt caaaggattt
      541 tatgagaaag gacgtggctt tgttcgagaa acagaagcta caccagaaga t
//
LOCUS       DACCSK010000258          585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCSK010000258 DACCSK010000000
VERSION     DACCSK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.272"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..>585)
                     /locus_tag="IVP39_002858"
     CDS             complement(<1..>585)
                     /locus_tag="IVP39_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP7587887.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGK
                     KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQ
                     TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVK"
BASE COUNT          159 a          138 c          106 g          182 t
ORIGIN      
        1 tttgacagat ttataggttg attccacaac ggcattatca tacggacaac ccttcctact
       61 caatgaacga gtaattccaa aaccattcag aatctcatga atggtttggt tatcaaattc
      121 ctttccccgg tctgtatgaa aaagcttgac gtctgttaaa gaatacggta tccgtccaaa
      181 ggcttctttt accaatgagg catctttctt ctcaccacag gaataaccaa taatttctcg
      241 attaaacaaa tcaagtatta agcagatata atgccacttt ttccccacgc gaacataagt
      301 aagatccgta acgatggctt ccaatggctg ttcttgtgta aaggtccgat ctaatacatt
      361 cgctgttttc gcttcattac aagctgttcg ttgcccttta aaatgagcga tcgtataggt
      421 agatgtcaat ccgcggcgtt tcatgattcg accgattcgg cgccgactga gttgaagccc
      481 acgctttgct aaacacttct tcaattttcg ggtaccgtaa gcctttcggt ttcgaataaa
      541 ctcttcctga acgatctctt ctaagtcgga ctcattttcg atcgg
//
LOCUS       DACCSK010000259          543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCSK010000259 DACCSK010000000
VERSION     DACCSK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.273"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
BASE COUNT          209 a           87 c           52 g          195 t
ORIGIN      
        1 atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg caaccaatag
       61 aaaaaaatac ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat
      121 tttgtgaagc attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc
      181 ttctattata aaagaagata tatattattt cttaattacc tactacctta aaccaaaatt
      241 taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat cccaattgtt
      301 atgcttttat aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat
      361 atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc
      421 aactaagtta tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact
      481 acaagcatgt agttttatct gttaattatg tactacctaa ataaattagt ttataattaa
      541 caa
//
LOCUS       DACCSK010000260          541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCSK010000260 DACCSK010000000
VERSION     DACCSK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.274"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..285
                     /locus_tag="IVP39_002859"
     CDS             <1..285
                     /locus_tag="IVP39_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7587888.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          161 a           94 c          121 g          165 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaagatcaag ccttgaatga gataaattct
      421 tgctcgaaag ttatggaaat ttcgatagcc gtaggccact tgtattgtgc aatagttttt
      481 tacaaaaaaa agggggtgcg gatatttttt tggacctttt taacaaatat atctttgctt
      541 c
//
LOCUS       DACCSK010000261          537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCSK010000261 DACCSK010000000
VERSION     DACCSK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.275"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>537
                     /locus_tag="IVP39_002860"
     CDS             <1..>537
                     /locus_tag="IVP39_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7587889.1"
                     /translation="KKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          184 a           91 c          109 g          153 t
ORIGIN      
        1 ataagaaaaa caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag
       61 aatatattca aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag
      121 cgtttcctat tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg
      181 attcttctga cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg
      241 gaaaacaaac tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa
      301 atgagtttgg tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa
      361 ttaccttagt tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag
      421 cgattgatat tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca
      481 aatccatcac ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatg
//
LOCUS       DACCSK010000262          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCSK010000262 DACCSK010000000
VERSION     DACCSK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.276"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            33..>534
                     /locus_tag="IVP39_002861"
     CDS             33..>534
                     /locus_tag="IVP39_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7587890.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYI"
BASE COUNT          195 a          100 c           87 g          152 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac attc
//
LOCUS       DACCSK010000263          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCSK010000263 DACCSK010000000
VERSION     DACCSK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.277"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>534
                     /locus_tag="IVP39_002862"
     rRNA            <1..>534
                     /locus_tag="IVP39_002862"
                     /product="23S ribosomal RNA"
BASE COUNT          161 a          116 c          155 g          102 t
ORIGIN      
        1 tttcttaagg acaagctgtg atggggaggg aaataatagt accgaagttc ctgatgtcac
       61 actgccgaga aaagcttcta gtgagaaaac agcggcccgt accgcaaacc gacacaggta
      121 gtcgaggaga gaatcctaag gtgagcgaga gaactctcgt taaggaactc ggcaaaatga
      181 ccccgtaact tcgggagaag gggtgctgat catacgatca gccgcagtga ataggcccaa
      241 gcgactgttt atcaaaaaca caggtctctg caaaatcgta agatgaagta taggggctga
      301 cgcctgcccg gtgctggaag gttaagagga gtgcttagcg caagcgaagg tacgaattga
      361 agccccagta aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg
      421 gtaagttccg acccgcacga aaggcgtaac gatttgggca ctgtctcaac gagagactcg
      481 gtgaaatttt agtacctgtg aagatgcagg ttacccgcga caggacggaa agac
//
LOCUS       DACCSK010000264          532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCSK010000264 DACCSK010000000
VERSION     DACCSK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.278"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(35..319)
                     /locus_tag="IVP39_002863"
     CDS             complement(35..319)
                     /locus_tag="IVP39_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7587891.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT          148 a          122 c           83 g          179 t
ORIGIN      
        1 agaggttgtg acacgtcttt tgtcacaacc tcttttattt ttgcttcact gtaactgcta
       61 caatatcacc gatttctttt ccgatgtttt tacgaatatc ttttcgtagt ccaaggatgt
      121 agcagactga accatctgga tttttcacgc ccatattcac gatactgcct tcatacggca
      181 cattgtcaaa agttgcttgg actttaactc ttccagcacc gaattctttt cttatatcat
      241 acggaaaaat aatatatgcc ccgcccttgc cgacttcgga agcatagatt tttgattcaa
      301 aagaataaat ttccctcatt ttatttctcc tattttttac atctagtata gcatagcaaa
      361 aaaaagaagg aactgtgaca aaagtcttgt cgcagttcct tttttcgaat aaacggtgtt
      421 ctcctgccaa ctcctcggtc acaagtcaca atagtcaaca aacatgagaa gctgtttttt
      481 gaaattgctc cttgtgactc agagttaaac ggaaggctca caacctcttc ct
//
LOCUS       DACCSK010000265          523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCSK010000265 DACCSK010000000
VERSION     DACCSK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.279"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            96..>523
                     /locus_tag="IVP39_002864"
     CDS             96..>523
                     /locus_tag="IVP39_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7587892.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALA"
BASE COUNT          173 a          100 c          102 g          148 t
ORIGIN      
        1 tttatgaaaa aataagaaat aagtccaatg acctagcgac ttctccactc atcaggtgta
       61 tgcttgtact atctatagaa aaggagtgaa tatttttgaa aagtctttcg gtccaagaat
      121 tatccaagaa ctttggtcac aaacaagccc tcagcgatgt cagttttagc ttatcccctg
      181 gcaaaatcgt tggattagta ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta
      241 ttttaggact gatccattac tcagatggaa caatctgtat tggtgaaaat gagatttctc
      301 cctctagtca taaagggttg gaacaagtag gagcattgat cgaatacccg ggtatctatc
      361 cattcttaac aggctatgat catctgaagt tattctctga aaccaacgat gtatctgcta
      421 tcgacactat cgtgaatcag ctcaagatgg aaaagtacat tcacaaaaaa gcgaaatcct
      481 actcgttggg aatgaaacaa aaattgggaa ttgctcttgc ctt
//
LOCUS       DACCSK010000266          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCSK010000266 DACCSK010000000
VERSION     DACCSK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.280"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>517
                     /locus_tag="IVP39_002865"
     CDS             <1..>517
                     /locus_tag="IVP39_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7587893.1"
                     /translation="KLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRG
                     LGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNA"
BASE COUNT          177 a          108 c           99 g          133 t
ORIGIN      
        1 aagcttttcc cgaatgctca gttgatcatc gatcgtttcc acatcgtcca acatattggc
       61 agaactttct tgaatcatcg agtgaaagaa accacggtac gacttaaagg aaattcccac
      121 tctcaacgag ggttagggaa aaaattgaag cgctattgga aactcctaca aaaagaagaa
      181 gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt ttaaagatta tttgactgaa
      241 agtgagattg ccgatcgact gttagctgct tgtcccaact tacgccaagg ctatcagtta
      301 taccaagaca ttctttatgc ggtaaagaaa agagatcagt cactatttga ggattgttta
      361 actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac gtttaaaaag
      421 tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc attggaatgc
      481 ttaaacaacc acatcaaagt attaaaacgc aatgcgt
//
LOCUS       DACCSK010000267          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCSK010000267 DACCSK010000000
VERSION     DACCSK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.denovo.281"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IVP39_002866"
     CDS             complement(<1..132)
                     /locus_tag="IVP39_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7587894.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACCSK010000268        19509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.047, whole genome shotgun sequence.
ACCESSION   DACCSK010000268 DACCSK010000000
VERSION     DACCSK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.047"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(41..364)
                     /locus_tag="IVP39_002867"
     CDS             complement(41..364)
                     /locus_tag="IVP39_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7587895.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(351..1058)
                     /locus_tag="IVP39_002868"
     CDS             complement(351..1058)
                     /locus_tag="IVP39_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7587896.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            1144..1485
                     /locus_tag="IVP39_002869"
     CDS             1144..1485
                     /locus_tag="IVP39_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP7587897.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(1656..2504)
                     /locus_tag="IVP39_002870"
     CDS             complement(1656..2504)
                     /locus_tag="IVP39_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587898.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            3102..3965
                     /locus_tag="IVP39_002871"
     CDS             3102..3965
                     /locus_tag="IVP39_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7587899.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            4065..4547
                     /locus_tag="IVP39_002872"
     CDS             4065..4547
                     /locus_tag="IVP39_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7587900.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LSESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(4810..5277)
                     /locus_tag="IVP39_002873"
     CDS             complement(4810..5277)
                     /locus_tag="IVP39_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7587901.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            5400..6251
                     /locus_tag="IVP39_002874"
     CDS             5400..6251
                     /locus_tag="IVP39_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7587902.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(6502..8460)
                     /locus_tag="IVP39_002875"
     CDS             complement(6502..8460)
                     /locus_tag="IVP39_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7587903.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSDKA"
     gene            complement(8457..9329)
                     /locus_tag="IVP39_002876"
     CDS             complement(8457..9329)
                     /locus_tag="IVP39_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7587904.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHTETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(9671..10492)
                     /locus_tag="IVP39_002877"
     CDS             complement(9671..10492)
                     /locus_tag="IVP39_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7587905.1"
                     /translation="MSRAAKSLHITQPTLSKQLKDLEEELGVTLFTRGSREIQLTDSG
                     RYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAISFISDSLHSLIKAHPDI
                     QFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLPAQDRWGLLIHRNHPLAK
                     QTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVGTYNLLYNASLMVDNGTF
                     GALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSPAARKFLDVLTEKYS"
     gene            10670..11239
                     /locus_tag="IVP39_002878"
     CDS             10670..11239
                     /locus_tag="IVP39_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7587906.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            11306..12043
                     /locus_tag="IVP39_002879"
     CDS             11306..12043
                     /locus_tag="IVP39_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7587907.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYSDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            12274..13542
                     /locus_tag="IVP39_002880"
     CDS             12274..13542
                     /locus_tag="IVP39_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7587908.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            14029..14493
                     /locus_tag="IVP39_002881"
     CDS             14029..14493
                     /locus_tag="IVP39_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7587909.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            14477..15106
                     /locus_tag="IVP39_002882"
     CDS             14477..15106
                     /locus_tag="IVP39_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7587910.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            15460..16938
                     /gene="msr"
                     /locus_tag="IVP39_002883"
     CDS             15460..16938
                     /gene="msr"
                     /locus_tag="IVP39_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7587911.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            16985..17431
                     /locus_tag="IVP39_002884"
     CDS             16985..17431
                     /locus_tag="IVP39_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7587912.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(17630..18145)
                     /locus_tag="IVP39_002885"
     CDS             complement(17630..18145)
                     /locus_tag="IVP39_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7587913.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            18262..19463
                     /locus_tag="IVP39_002886"
                     /pseudo
     CDS             18262..19463
                     /locus_tag="IVP39_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6175 a         3475 c         4007 g         5852 t
ORIGIN      
        1 atgccttttt atttttctaa cttaatttta caattcgcgt ttataataag cgaatcaacg
       61 aaagggaaag aattcctgcc aagacgatca ccaataagct ttttgaaaga atagcagcta
      121 aaagtgtagg aactgaagcc aaagcatttt cgagattgat ctgcggcaga tgtccgatat
      181 tttgggtaaa tagactgctg aaccataaag cagacataat gacgatcggg acaaaactta
      241 aatactcaag caacggttgc ggtaaatcga attttttcag taagacaaat ggtaggaccc
      301 tagaaagcca agttgctaaa gtacaaccta aaatagtcag caatgtaaac tcaaaagaag
      361 gcatgtttga tccacacccc caatccgcac ccaatcagtg ttacaactac aatcactaag
      421 ttgcttggga taaaaatcag tccgacatat accagaatca aagtgagacc aatcaggatc
      481 aactgtaatc gtttagaggt gtttctatca gaaatgactt gaagataaag taacccgata
      541 aacatagcga cgatggcgaa atccaaacca aactttttcg gatcagtaat aaaattccct
      601 aaaagtgcgc caacaagaga agaacccacc cacgtcaaat aagaaatcca attagctgta
      661 ttcatccaac gaaagtttaa cttttgtcca gtatagttca atttattcat tcccagtgcg
      721 aaactttcat ctgtcagcaa tgtgccaatc agcagatttt gaagcagccg atttttcttg
      781 aaataaggag caatcgtcat actcatcaga atcattcggg aattgactag aaaggtcgag
      841 aaaacgatgg aaagcaatgg gctgtgacta gcaagcatgc tgactgtgat aaattgggca
      901 gaaccagcat agaccagcag ggacatcaac gtgacgacta gtggatgaaa tccggcagct
      961 ttcccaacga taccaaatgc aagtccaata ccgatataac cgaaaacggt aggtaacgtg
     1021 tctttgatcg cagtttttat atctaacttt tcatccaata acaaaactcc tttagcacga
     1081 cataaatttt cataattgtc gttaaacttg tgaatattct aaatttttaa tgagcatttg
     1141 tcaatggtgg gattgaaacg taaagaacta gcgggtcgca gatttacacg tattgtttcg
     1201 gtgggaggag gcgcgaaaaa tcttgcatgg ctacaaatcc aagctgatat tttcgatgca
     1261 acggtctatt gtttaagtgt ggaggaagga cctgcattag gcgcaacaat gattgctgca
     1321 ctagggttag ggtggttcga cacacctgaa agttgttgtc agaattttat ccaatataca
     1381 gatgcagtgg aacctctctt acaaaatgta gaaaagtatc ataaaatcta tcaacaatgc
     1441 cggaaaatct atccagcaac gaaggaaatt tgtcatgact tataaaacaa aacgcgtacc
     1501 taatagtcaa atgactaata ttgggtacgc gttaagtctt ctttacttca tcctttgcat
     1561 gattcgtgtt tttaaagcaa ggtctgctgt atttcttgtg gatttaggat ggttgttatc
     1621 gtttacttgc tatttttcat tgaaatcgtc tttctttacc gtttctttag ttttttatct
     1681 atttttttct tttggttaga aaattctttt tcccgaaagt cttctttcag gatttttatg
     1741 gagtagcgtt ttgaataaga ggcgtcagta tgatcgactt ggcagacgaa ccaagagaac
     1801 atttgccttt tttctgcatg aggtgcttct gctaataatt ctttccataa ataataacag
     1861 tcctctatca gaagaagggt tcgttcatcc tcaggctccc aatagaatgt ttcgagagta
     1921 gcaatcactt cattgatcaa ttcgaatgcg ggaagatagg cattttgctg aataagcgat
     1981 tcaactgctt cttggatgaa ctttcgcatc tccatgatga acttcgctgt tttcctgttt
     2041 ttgtttttct tgataatatg atcgtattta tggatgatag ttgttaattt atctgaataa
     2101 tttattgcat gaaatggcat cttaactttg aagcgttcga taaagtgcgg atgttctata
     2161 agaatctctg ttaaaagctc ccgtacaaga gaggaactag cggaggaaac aagggacgag
     2221 agttcggaaa gtgagtagtt ggattttttt attggacgtt cttcgtaagc gaataagaca
     2281 gcagccatgt gtttgcattt ttttccttct atggcatata gacaatcaca agtcatgttg
     2341 gtcttttcat cccaagtgat cactacctta taatcttccg tgccagaaac tgttgcggac
     2401 aagtacttgc ttgtatagtt caaatgacgg acaaaaccgt gcagatgata gctgtaaccg
     2461 cgttccaaaa cgttttcagg aaaatactca agccaattat tcatctagtt ctcctttcat
     2521 gaaacactta tttatattta gtataaagaa acgtttaaga aaaaagtagc tgactttaca
     2581 tatttgataa tcacttttgc tcgaattgtc aaaaattcat tctccttttc ttttcttaga
     2641 gaatgtgaga aaaagtaaaa gaatagcgtg ttttaaagat ggaacttttt ttgtttttta
     2701 tttatctgac aaaaatgaag tgattttgtt agaaataaat aattgttcca attgataaaa
     2761 gaggtatgat gtatcgcaaa gaaaaatcca agcagcgttc tgatcagcag tttgaacaac
     2821 aatttgcttt gtacagtagg tagtaagcgg attaactaag ggaataaaag atatttttac
     2881 cgctttgttt acttagaatc tgtcttcccc aaaatttatt tttctaaagg taaaacatag
     2941 ataggcagat aataccaatt atccgttacg aaacgctgct tgagaatttt ctttagatcc
     3001 atacaacatt ttctacatct gactaaattg gttcctgatt catcaagtaa ttgactttct
     3061 ttgataatat gataaaaagg caagcaagga ggaagataaa tatgcttaaa gatctgaacc
     3121 aattgatcga ttacatcgaa gaacatttga cagaaggact ttcctataaa ataatagccg
     3181 aaaatacagg aatctctgaa tatcaattaa aacgcatctt tacgtttatt gcagggatgt
     3241 cgctaatgga ctatatcaaa aaccgtagat tggctttggc taatcgcgat cttctagatg
     3301 gatcaagagt gacagaaacg gcgtttagat atggatatga aacagtcgaa ggtttttcaa
     3361 gagcttttcg tgattggtca ggatatctgc cttcagaaat cagcagatac ggcatacaaa
     3421 aatcttttcc acgcttgaca ttttttattg atgtcagagg aggaaaatcg atggaatttc
     3481 gtatcgaaaa gaaagaacag ttttatgtgg caggtgtggc gaaaaaagtt cctgtcaaat
     3541 ttgaaggagt caatcaagaa atacaaaagt tagcgcagac gatcacggag actcaaaggg
     3601 aagagatgca taagttagga gatttatatc caaatcaagt aatcaacgtt tcttatggat
     3661 ttgatggaga ccgcttggaa gaaaagggca gtttgatgca catgattggc tttgctacga
     3721 caaaagaaaa tccgtacgct gatttagaaa tgcttgctat agaagaacac acttgggcca
     3781 tttttcctaa taaagggcca tttccccaaa ctttacaaga tacttggggg aaaatctatg
     3841 cagaatggct tccttcttca gattatgagt tagtcgaagc tcccgagatc tcttttacaa
     3901 aatggggaga ggatttcagt aatgtttaca gtgagatttg gattgcagtg aaagcaaaaa
     3961 aatagttaaa aaataaaaat atccctagaa aaaagagcgt aatcgtgtca tttcccttta
     4021 attttatgta agatgaactt aatataatag aaagggtggg agaaatggca aagtatagga
     4081 tcgaagaaaa accagatttt acggtgacgg gtattggtgt ggaattgaag agtagcaata
     4141 cggattttgt cggtttggct gcagagaaac aagcatttga gcaaaaagtt atcggtaatg
     4201 gaacaataga tgcattgaaa caagctgctg aaaatcatta tttctttaca gtcaacgaac
     4261 cctataacgg gaaaatgatg tactatgtcg gtgttcaaac gagtcaggat gttccaaaca
     4321 ctacgcgaat catcccattc ccaaaaggaa cgtatcttgt tgtaagcgct ttatcagaat
     4381 cgcctgaaga attaagaaat gatttgacga atacggcctt tgggcatgtt ttaagagaag
     4441 tagaagatta cgcctatgtt gggggtccaa atgctgtagt cgaaatggga gttaccaacg
     4501 ggaaatatat gggagaaatc tggataccgg tagtcaataa accatgataa tatagaggtc
     4561 gtgaaagtag cgctttgacc tgagcattag agaggaaaaa tgggaaatag ctttccatat
     4621 tttgcatttt tcagaactaa tgccgagggt cagctacttg aacaccgttt atcaagacgt
     4681 catgacttca gcgctttgac ctgagcatta gagaggaaaa aggggcatga caaggctttt
     4741 gtcccccgga gaataaaaga atttatgtag acgattccga tcagaaaaat gcaggcacag
     4801 tagagagaat tataaaaatc tctctacgag gcctgtgttt tctggtcttt tttctttttc
     4861 caaaacggct attttatgta tcagactatc aagtgtgatc ttagccattt cttcttctgc
     4921 ctttttctgc agccgagcgt agctttcttc taaagcttct tggatattcg caccaacgac
     4981 gcaatttgga tttgtttttg gatcgacttg gataagctgt gtattacctt cgatgctgcg
     5041 atagacatct agtaaagaaa tctgatctgg acttttggca agggaaggct gcggttttcc
     5101 ggtttttgta aagatcaaac cggatttttt gagctgactc atgattttac gtacatttgc
     5161 tgcatttgtt tcgatactac tggcaatcat ctcactagaa agtgatcggg tatcttgaaa
     5221 catttcaata taggcaagta catggattgc atcactaaat tgcagagaat acttcatcag
     5281 ttgtcatcct ttcatttttt ttgcttgact tatcttacca cactctttat gataaaggtg
     5341 taatttataa atgtacagct gtaaatctta acaaataaaa agaataggga gtggcaaata
     5401 tgcattattt agtaacagga gcaactggag gttttggtgg ttatgcgctg gaattcttaa
     5461 aaaagatggt tccacaaaat gaaatctatg tactagtacg tagtgaagaa aaaggagaag
     5521 cactgaaaga agctggattt aatatacgta ttggtgatta tgatgattta gactcaatga
     5581 aagaagcgtt gaaaggaata gatcgattgc tttttgtctc tggcgttcca ggaaatcgtc
     5641 aagccgaaca taagaatgta gtgaaggcag cacaagcagc tggggtatct tatatcgctt
     5701 atacaagttt tgcagatgcc gatcactcga tcagtgtatt agcgccagat caccaattca
     5761 ctgaaaaaat gattaaggat tcaggtattt cccatacatt tttacggaat aattggtatg
     5821 tagagaatga attacctatc attgggcagg ctttaaaaac tggtcaattc gtttatgctg
     5881 caggaaatgg aaaaacaggt tgggcattga aacgtgaata tgcagaagct gcagcaaaag
     5941 tactagtaga aagtgactct ccagaaatct tagaattatc cggcaatcta actgactatg
     6001 aagaattagc caaggcatta gagcgtgcga caggtaaaga gttagaaatt attgaagcaa
     6061 gtgatgcagc gtttgtcgaa aatctaaaag aagccggatt ccctcaagaa gcagctgaca
     6121 tgtttttagc gatccaacat gatattaaaa aggatcagtt agctatttcc tctgatgatt
     6181 tagaaaaagt attgggaaaa ccattaactt ctcttgaagc agcattaaaa gaaatattgg
     6241 atcaggaata atgtataaag aggttgtgaa aaagccgatt agttttgagt atgaagaaaa
     6301 attttgtgaa aaagctgttc tgcatcaagt aataagaaga gtcaaataaa aaatagctct
     6361 tcatatgttt cgtttggctg gacttattac cgcggagttg gcaggagaac gccgttttta
     6421 ttcggaaaaa ggagctgaga caagattttt gtctcagctc ctgtttgttt atcgataaaa
     6481 aagtctgggg aagacgatgg ctcaggcttt gtcggaaaca gagccgattt tatcatttgc
     6541 ttttacatca cgtgcgggtt cgatttctaa gctttggact ttgtcgccat ttgtgaatac
     6601 aacgaccata tccgtgtttt tgcctgcaga ttgaatagcg gcaagatcaa ctaaagcaat
     6661 caattgacct cttgcgactt tttgaccttc ttctacatat aaagtgaatg gttcgccttt
     6721 taagtcaaca gtgttgatgc ccatatgaag caagacttcg ataccagcat ctgtttggat
     6781 tccgacagca tgtttcgttg ggaaaatgtt tgtgatcgta ccagcgattg gagagaagat
     6841 ttctccattt tctggtaata cagcatagcc gtcacccatc attttttccg cgaatacatc
     6901 atctgaaact tcagtgattg gaatcagttt cccatttgcg atagcgtaaa tatcttgttc
     6961 gatcgtttca ccggcaaatg tttctgtttt aaccacggtg tttgtttgtg caggtcgtgc
     7021 tgcagcacca ccatgtagtt ttgccatctc atctgcaacg aattgtactt ctgtacctac
     7081 gatgacttgc gcattttttc cgtcgatgat cttcatacca ggtacgccag tggctttgat
     7141 tttattttga tcgatcaggc ctgtatcttt gacttgcaaa cgaaggcgtg tagtacagtt
     7201 atcgatcgaa gttacattgt cggctccgcc taatgcagcg tagattttgc ttgcttgtgc
     7261 cgcatattta ttgtctgaat tagtcgttgc agtatcgtca tctgaaagat ctgcttcctc
     7321 gcggccagga gtcatcagat ggaatttttt gatagcaaag ttaaagccaa agtagtaaat
     7381 cactgctgtt actaatcctt ggacgatcaa catcaatggc tgattcgcaa tcggattttt
     7441 caaagatagg aagtaatcga caaatccggc actaaaggca aatcctgctg tccaatggaa
     7501 gaatgcactc actgctaaag aaatcccagt gaaaattgca tgcaagacat ataatggcca
     7561 agcaacgaac ataaatgaaa attcaagtgg ttctgtcaca ccagtaaaga atgaagcaaa
     7621 agctgctgct agcatcaagg aagctgtttt tgctttgttt tctggacgtg catttctgta
     7681 aatagcatat gctcctgctg gaagcccgaa catcatgatt gggaaaaatc cagcttgata
     7741 cattcctgtt acgccttttg ttccttcaga agccaaaaat ttaccaatat cattgattcc
     7801 tgctacgtca aaccagaaca cggagttcat tgcttgatgc aatcctgtag ggatcagtaa
     7861 acggttgaag aagccataaa gacctgcacc accccagcct aaactcaaaa tgaatttagc
     7921 aaaagaaact aaagcagagt aaaccggagg ccatacgagc agtagcactg cactgatcag
     7981 caacatgaca aaggcagcca agattggcac tagacgcttg ccgctaaaga aagataaagc
     8041 catcggtaat tttgcttcgt ggaaacgatt ataaagagct gccgcaatca atccggcaat
     8101 aataccaata aatacgttac tgatctgagc aaaagatgga tcaactgatt ctggtttgat
     8161 attcaataaa gtagctactg aatcagtttt taaaacattt actggaactt caaatgcgac
     8221 caaacctgcc aaggcagcag cacctgattt gtccactgac atccctagtg ctaacccaac
     8281 ggcaaacagc aaaggtaatt gacctaaaac tgcgttaccg ccagcttgta aaaaatgagc
     8341 gacaatgtcg ttactataag atgcccacca gtttccgatc cctactagta aagcagcagc
     8401 aggtaagacc gcaactggaa gttgaaggga acgtcccatt cgttgtagat atgctttcaa
     8461 tttttttcct cctcatgatt gttaacattt gtttgtacga ttgcgcttct taaacgctcg
     8521 atatggatgg ccaagtaacc cagttcttct ttcgtgatcg cgagctggta ttctttggtc
     8581 agcaacacac ggattttttt agaaatctta tagctttctg ggtattttcg tttgaccatt
     8641 tctacgattt ccgtgtctaa aacagcatat tgttgcgcat gggttcgttg tagaagcagt
     8701 cgtaaatgat tcaccagtct tgaataattc agtgaaaatg gttctgaatg gatatctgtt
     8761 tccaattctg attccactaa atggatgatg tcagatacaa tcgtcacttc acggatactg
     8821 cgatggttat tgttccgacc gcttcgtccg ctgtgaatat ggattgcgat atatcctgct
     8881 tcgtcataag tatatggaat cccaagctct tttgtcagat actctactgc ccattgagca
     8941 atactgaatt catcactgta aagcacttcg atttctctta aaagtttgtt gcggatgacg
     9001 ataccgttat taatgttctc cgcagaaaaa gcaatgtggt cggtcaaagc gatcaataca
     9061 tgttcattca acttttccat caaaacagtt tccgtatgat caatgatcgt ttctgctgca
     9121 aataaaaagc gttcatcaat ctgattaagt aaattttgca acttgatttg accttctggt
     9181 tccatgacaa atatccgttc gacatcacga tcaaaaatca aatcatttct ttttctgtca
     9241 aatccaatcc cttttccaat agctactttt tcctgtccac cgtcatctac caatactgca
     9301 ttttgattaa agatcttttt gattttcatg cgcgcaccct ttttgaagat ttggttttca
     9361 cgtttgctag accagttgat tgcggccaaa caaaaataga cacgaaaaac ctcctaggga
     9421 aaccatgttc ctcagagatt tttcatgcct agtatgatct agtcacatga aacgaattat
     9481 tgttaacgta atcaatttta tatagacagt tatgttttgt caattctttt tttataatta
     9541 tagtaattta ctggaattat cagaaataaa aggaagaaat aaattaagaa acttatgtta
     9601 tttaatcgtt ggatcttttg cttatcttat tcgttgttaa aaagctgaaa gcgaaggaaa
     9661 aaaactctac ttaactgtat ttctccgtta ggacatctaa aaatttacga gcagcaggag
     9721 aaagaggctg gtcttttttc caaatgagat tcagtgaagc ttgtaatggc ggatcgagtg
     9781 ggatgaatat tttttccgtc ccataagtat tgatgatccc atctagagct aatgcaccaa
     9841 aagtcccgtt atcgaccatc aaagaagcat tgtacaataa gttatatgtt ccgacgatcg
     9901 ataactgatc tatgctgaaa cctagccatt cagctagttg actgtccact gacgtttgcc
     9961 gggaaatgat caaaggaaca gttttcagtt cctgcggatg gatgacggtt tgctttgcta
    10021 aaggatgatt tcgatgtatc aaaagtcccc aacgatcttg ggctggcagt cgaaatgact
    10081 ggtatttttg tttgtttgtc ggttcgatta cgagtccaaa atcaagtaat ccgctatcca
    10141 gtttgtctat gatatcatct gcattcccgc tatataaatg aaactggata tcaggatgcg
    10201 ccttgatcaa cgaatgaagg ctatcagaaa tgaaagagat tgctttagtt tcgcctccgc
    10261 caatgtagat ttctccagag atgattgcgt catttgttaa attgttcgcc gttttttcta
    10321 ccaacgacaa gatttctttt ccttttgaat aaagatatcg accagaatcc gtcaactgta
    10381 tctctcggga gccccgagtg aaaagtgtca caccaagttc ttcttctaaa tctttcagtt
    10441 gttttgagag agtaggttgc gtgatatgca gagattttgc tgctcggctg atattttttt
    10501 ttctagctac tgctaaaaat acgtcagtat acgaatatcc attttttccc tcctgttttt
    10561 aaaaaagcat accactaagt atagttaaaa ggaatagtta agtatgtttt ataagtatta
    10621 gacatgataa gccgtccgtt ataaagtaag gataaaagga gggagcaaaa tgacaagcct
    10681 catcgaaaaa caaatcgtca ataaagagat tcgagtaacg gatccgctct ttgaagtgat
    10741 ccatcagatc caagctgaaa acgaagagcc tttagcagcg ctgaatactg ggtaccatga
    10801 accagcagat atccgcaaac ggctagaaaa tatcattagt gacaaagtag atgatacggt
    10861 gactgttctg ctgccttttt atacagattt cggcaaacat attagtatag ggaaaaatgt
    10921 ctttatcaat cgtcaagtga tgttcgttga cttaggtggc atctgtttag aagattcagt
    10981 attgattggt cctcgtgtca acctcatcac ggtgaaccat ctaaccgatc caaaagaacg
    11041 aagaggcttg tcagtaaagc caatccacat caagaaaaat gcttggatcg gagcaggagc
    11101 aactattctg ccaggcgtaa cgattggcga gaatgccatt gttgcagcga atgcaactgt
    11161 tacgaaagat gtcccagaca ataccatcgt tgcaggaatc cctgccaaaa tcgtcaaacc
    11221 tgtcgaacgg attatttgaa cagtaagatg aaataagaga tagtgagtat aagcaagaga
    11281 tttttcaaaa caggaggaga atagaatgaa tgatttatca gaaaaagtga ttgtcatcat
    11341 gggcgcttca agcggaatcg gtgaagcaac aacgaagcta ttagcagaaa aaggggctaa
    11401 actagttata gctgcacgcc gagaagatcg cctaaaagca ataaaagaat ctttaccaga
    11461 agctgagcta tatattcaaa cagcagatgt aagagatttt gcacaagtcc aagcagttat
    11521 cgatttagcg atggaaaagt tcggtcgaat cgatgtattg tacaataatg caggaatcat
    11581 gccaaccgct ccattagttg aaggtcaccg tgatgaatgg caaaatatgc ttgatatcaa
    11641 catcatgggt gtgttaaatg gaatttccgc cgtacttcca atcatggaaa aacaaaaatc
    11701 tgggcatatc atttcaactg attctgtggc gggacatgtt gtttattctg attccgccgt
    11761 ttattgcgga acaaaatttg ctgtccgtgc aatcatggaa ggattacgtc aagagcaacg
    11821 gcaaaataac atcaaatcta cgatcatctc tccaggagct gttcaaactg aattatatca
    11881 aaccatcagc gacaaaaagg ctgctcttga acttcatgaa gcgcaaaaag aatggggatt
    11941 gacctccgaa gatatagctt ctgctgtagc atttgcgata gaaacgccag accggatgtc
    12001 tgtcagtaac atgattatcc gtccgacgac acaggaagta taatccataa cggataatat
    12061 ccaaagagaa ttacttgtta aatagttatt caaacgacca tcaatttaag gcgtgaatgg
    12121 agaagaatat tcttcattta cgtcttttta tgtagaaaaa gcaaatttat caaaaagaaa
    12181 caaatgttat ttaaatatgg acattgactt gcttttactt ttttagttac actgttattt
    12241 aggaaaagtt tcataattca aaggagaaaa aagatgaaag caaagaattt attatttagc
    12301 gttattcttt taggaaatct agcagttgcc aacacaacag tgctagctga agaatcgaca
    12361 gaaactgtcg caactcagac atcgagttct tcagaaacag gaattgaaaa cgatacagag
    12421 agtactttgc agtcagctac tgtctcagaa aatacaattt caaccgaaga accaacaact
    12481 gaaacaacag aacaaacaca aaccgaagac agtacatcag cagaagctca gaacaatcca
    12541 gagaatgcag aagaaatcac gaaccaattg acagatgggc aaacattgac acatacccat
    12601 caagcagatt tgtctgaatt cgaaaactat actggctatg ctttttatgc tcgctcatct
    12661 gctaattctc aacaagcgtt cattgatagt attgcctcaa ctgcacaaag tttggcaagt
    12721 gccaatgact tatatgcatc agtcatgatt gctcaagcca tcgttgaaag tggctggggg
    12781 aacagtactt tggcttctgc ccctaattac aatctatttg ggattaaagg aagctataat
    12841 ggccaatctg ttacgatgcc tacttcggaa tatgtgaatg ggcaatggat tacggtgaat
    12901 gccgctttta gaaaatatcc ttcgtacaaa gaatcattgc aagataacgt aacggtattg
    12961 aagacgactt cttttcagcc aggcgtttac tactattccg gtgcatggaa aagcaatacg
    13021 aactcttaca aagatgctac ggcttggcta acaggtcgtt atgccactgc gcctaattat
    13081 ggttctacat tgaacaatgt aatcgaaacc tataatctga cacagtatga tacagcacca
    13141 acttcaactt ctccgatgta tcgtctgtat aaccgtcata caggagaaca tttatataca
    13201 ttgaatgccg gagaaaaaaa ttatctgcca actgtaggct gggaatatga agggattgct
    13261 tggcaagcac caaacagcgg acaacctgtc taccgtttat acaatcctta cagtggagac
    13321 catcattaca caatggcaca atctgagatc aactctttaa caaaaatcgg ttggcgttat
    13381 gaaggtctaa gtttttattc tggtggttcc aagccaattt atcgactatt taatccaaat
    13441 gaaaaaactg gtacccatca ctataccttg agtgctaagg aaagagattg gctaacaccg
    13501 atgggctgga gatatgaagg aataggattt tatggttatt aaaaagaggt agtgaaaaag
    13561 ctgccctgca tcaagtagta agaacagcca aacaaaaaca gattttcatg ttttttgttt
    13621 ggttgaactt attaccgcag atgcaagttt ttgaacaccg tttactcaaa aaaggttgtg
    13681 acaacatctt gtcataactt ttttattact gtttaagcat actgatccaa tttctaatat
    13741 tcgacgggat tcttgcaaaa ggcgatttcg gaaatttttg gcggtgtaaa attttctact
    13801 gcagaagagc tatcttctat agtttgataa gaatcaataa tcttggtttt agcgttgtgc
    13861 tttctttatc attttttccg ttcattctac aaaaaatttc ttttctctat acttttctat
    13921 tgagagcgtt ttttttatta cacagctgat tctttgactt ttttcttttt ttattctatt
    13981 attgacaagt caatcgttga cgagtcaatt attagaaagg aggaaattat gtctttagaa
    14041 gagattagtc accttttata tcaaatcaaa ttagccgatc aaaaaatcgt ccagatcttt
    14101 gaagaaaata tcggtataag tcttactaga tacgagatca tgatgagact gaaagaagaa
    14161 aatccattgc tacaaagcag tttgcaagaa aagttgcaga ttgatcaagg agcagttact
    14221 cgtcatctga aggtattgga agcaaatggc tatgtttcgc gtcagagaaa tcctcagaac
    14281 aaccgggaag tgtatgtagc gctaacagaa aaagcccaga agaaaatcga gcattgtgaa
    14341 atggaccata aagatacagg ttccgtgcta tccccggctt tcagcgatgt agaaatgaaa
    14401 caatttctaa cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg
    14461 aaagaggtta ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag
    14521 atccgttcgc gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat
    14581 caaagaagcg acgactgcac cttcttctgt caatatgcaa ccatggcgct ttgtggtcgc
    14641 tgaaagcgag gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa
    14701 tgatacttct tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga
    14761 agaaatctat aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca
    14821 attagctgcg atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt
    14881 caaagtcgat tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta
    14941 tgaaacaaat ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga
    15001 taaagaccgt tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta
    15061 cgagtctgtt cgtttagcac cagaaaagat cactacgttc gaataaaagg ttttcagtta
    15121 ctattcagta gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc
    15181 ggaaaaatga ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat
    15241 tcaaaagaca tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac
    15301 agtagaatag gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga
    15361 gtaggaaaag atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc
    15421 gataattgtt gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat
    15481 ataacaaatc tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt
    15541 gtctatcagc aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg
    15601 ctcaaactaa tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc
    15661 ttcaactacc tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta
    15721 gccagtcatt tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa
    15781 gaacgaaaga tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac
    15841 gagccaacaa cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat
    15901 tattatggta cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg
    15961 aaaatctggg aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat
    16021 ctcactcaaa aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa
    16081 gagaaaaaac gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa
    16141 aaagtgtcca gtaaaaagaa aaagcaacaa atcagaccgg atcggttgtc ttcctctaaa
    16201 caaaaagaca gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc
    16261 caaaaaatag gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca
    16321 aaatctcttg aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga
    16381 agtgggagaa cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg
    16441 attgtcggtg aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga
    16501 gaaggaatcc ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa
    16561 atgacatctg aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg
    16621 cttgtccgta gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta
    16681 tgtacattaa gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca
    16741 agtaatgtgt tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct
    16801 ttagagaagc taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac
    16861 tttgtcgagc gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta
    16921 cttacgagaa atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta
    16981 cagattgcct acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat
    17041 ggtcttgggg atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt
    17101 gttgattggt ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg
    17161 tgtgctggcg aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat
    17221 gatcttaact atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg
    17281 tgtgatcatg gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa
    17341 aatgaatgtg attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag
    17401 tgtgctaaaa gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaaaggcctt
    17461 ggcatgcttt ttgtcatagt ctcttgaatg cagttctaga aaaatagtga tccgaacgat
    17521 aggttttggc ctgtcgttcg gatcgctttt tcttttccaa ctgtattgct cttttattga
    17581 agataatttg ctcttcaata gatatttcta gaaaggttct gtttatccct tactcaaaaa
    17641 actgctgcat ttgttctctt acttgctgac accattggag ttcctgctga caaagcataa
    17701 ttctgcgctg gatcacaaca taacgtccaa attcttgttt cttgtctagc gagaggtcat
    17761 tcaatttttt ttgatttttt tgcatgattt tgttcagctg ttgttctcta gtgaatagca
    17821 gtccagtagc tgttgtttta tcgagcgtcg aaaaagcata gatttttgct agaaattcat
    17881 ctttttgcgt aggagcggga gtttcctctt tgatccattc ctctacaaga gcacgtcctt
    17941 catcagtcaa gtggtagatc tttttttgag agtgctcgtc tttgtccaaa acggtcacat
    18001 aaccctgctg gtctaacttg gcaagcgttg tatagatctg actgtgatgg gcttcccaaa
    18061 aaattgtgat cagctgcttc aattcatacc cagaacgtgg tttggataaa agcacgcata
    18121 ataatatata ggctaaagta ttcataaaaa tcctacctct cgtcttattt tctttattat
    18181 atcacagtaa agtttccacg ttttacatgt gcatatttac atgtaaaata aatcatgtat
    18241 gatgctaata gagagaaagg gatggaaata ttggagaatc aaatagatat acaagaacaa
    18301 acaaatgaag aaacaaaggg gaaaagttca gcgctagttg tagcagctac ctgtatgatc
    18361 gcttttatgg gagtgggtct agtagaccca atcttaaaaa cgattgctgc acagttaaac
    18421 gctacgccag ctgaaacgac cctgttattt actagttaca tgttagtcac tggtgtggtg
    18481 atgctattta caggtttctt atctagtcgg atcggtttga aaaaacattg atgacaggat
    18541 tgtttatcat cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga
    18601 ttgggttacg tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa
    18661 cgatcgtcag tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac
    18721 ttggctgcgg aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc
    18781 gttatccatt ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac
    18841 ttttacctga aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt
    18901 taagcaatca taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct
    18961 ttactttact tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt
    19021 ttgtattttt tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc
    19081 ttgagcggaa gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt
    19141 gtttgctgtt attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt
    19201 tagcaggatt tttccaagga attatcaata cactgttgac gacgattgct atggaaatac
    19261 caggtctgca acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag
    19321 ctttagcacc atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt
    19381 attttgcagg tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt
    19441 ttgctacaaa tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag
    19501 tcataagaa
//
LOCUS       DACCSK010000269        11622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.086, whole genome shotgun sequence.
ACCESSION   DACCSK010000269 DACCSK010000000
VERSION     DACCSK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.086"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..385
                     /locus_tag="IVP39_002887"
     CDS             <1..385
                     /locus_tag="IVP39_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587914.1"
                     /translation="EAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKM
                     KNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVM
                     QFHPIFEELLHAFDTRPPVRNEVEE"
     gene            382..2409
                     /locus_tag="IVP39_002888"
     CDS             382..2409
                     /locus_tag="IVP39_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587915.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            2406..3428
                     /locus_tag="IVP39_002889"
     CDS             2406..3428
                     /locus_tag="IVP39_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7587916.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            3445..4374
                     /locus_tag="IVP39_002890"
     CDS             3445..4374
                     /locus_tag="IVP39_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7587917.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            4619..4735
                     /locus_tag="IVP39_002891"
     CDS             4619..4735
                     /locus_tag="IVP39_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7587918.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            4751..5554
                     /locus_tag="IVP39_002892"
     CDS             4751..5554
                     /locus_tag="IVP39_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7587919.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            5729..6025
                     /locus_tag="IVP39_002893"
     CDS             5729..6025
                     /locus_tag="IVP39_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587920.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            6032..7984
                     /locus_tag="IVP39_002894"
     CDS             6032..7984
                     /locus_tag="IVP39_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7587921.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(8056..8553)
                     /gene="dfrG"
                     /locus_tag="IVP39_002895"
     CDS             complement(8056..8553)
                     /gene="dfrG"
                     /locus_tag="IVP39_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7587922.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            8915..9955
                     /locus_tag="IVP39_002896"
     CDS             8915..9955
                     /locus_tag="IVP39_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7587923.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            10053..10241
                     /locus_tag="IVP39_002897"
     CDS             10053..10241
                     /locus_tag="IVP39_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7587924.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(10299..10652)
                     /locus_tag="IVP39_002898"
     CDS             complement(10299..10652)
                     /locus_tag="IVP39_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587925.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            11183..11605
                     /locus_tag="IVP39_002899"
     CDS             11183..11605
                     /locus_tag="IVP39_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7587926.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
BASE COUNT         3960 a         1943 c         2207 g         3512 t
ORIGIN      
        1 tgaggcttgg agttttttac aagtggcaca aggaaaagct ctatctatgc gattggttcg
       61 agcaggacgt gccatgaatg caggggtcta tttcgttact caaaacgcgg acgatttact
      121 agatgaaaag atgaagaata atattggctt gaaatttgcc tttcgttcca ctgacctagt
      181 ggaaatcaaa aagaccttag aattttttgg tgtggacaag gaagatgaaa acaatcagaa
      241 acgcttacgt gaattagaaa atggacaatg tttagtttca gacatctatg gtcgtgtagg
      301 ggttatgcag tttcacccga tttttgaaga gttgcttcat gcctttgata ccagaccgcc
      361 tgtgcgaaat gaggtggaag aatgacacaa aaacaaaaga aatttcttca ctatagttgg
      421 ataacgctcc tttgtgtggg cgttatttta cttttattag gcacgctggg caatgcagta
      481 caagcgacag gcttagttga tgaaaccatt gatacaagca atgaatactc caagtatggt
      541 ttgaatcaat atcagcttga ttattatgtg gacaatagct ggggctggct tccatggaat
      601 tggagtgatg gcataggaca atcggtgatg tatggattgt atgccattac taattttatt
      661 tggacaattt ctctttatct atcgaacgcc acaggttatt tggtgaaaga agcctatgcc
      721 ttagatttca ttagtcaaac atcccaagca attggcgaaa acatgcagac gattgcaggc
      781 attacaccga atggcttttc cagttcaggg ttttatgtcg gtttcctcct tctttttatt
      841 ctcattgttg gaatctatgt tgcctataca ggattggtta agcgagaaac gacgaaagtg
      901 atacgagctg ttttaaactt tgtcgtggtg tttattctat cggcttcctt tattgcgtat
      961 gcacccgatt atattgcgaa aatcaatgat ttttccaaag atgtcagtac cgccagtctg
     1021 aatattggga caaaaattgt tcttcctaac acggatagtc aagggaaaga cagtgtggac
     1081 atgatacgtg acagcttatt ctctgttcaa gtaaagcaac catggctatt gcttcaatat
     1141 ggctccactg atgtagaagc gttaggggaa gaacgtgtgg aaagtttatt gtctaccagt
     1201 ccagatacaa acaatggtga agatagagaa aatgtagtga ttgaagaaat tgaagatcat
     1261 gacaatacct atttaaccct accgaagacg atttccagat taggaacagt cttctttttg
     1321 tttgtattca atattggtat ctcgattttt gttttccttc taacaggaat catgattttt
     1381 tcgcaagtgc tatttattat tttcgctatg tttttgccaa tcagtttcct attaagtatg
     1441 ttgccgagtt ttgatggcat gggcaggcat gccattacca agttatttaa tgtgattatg
     1501 acaagggctg gtattacctt gattattacc gttgccttta gtatttccac tatgctttat
     1561 tcgctgtcag caagttctcc ctttttcatg attatgtttt tacaaattgt gacctttgca
     1621 gggatttatt ttaaactggg cgatttaatg agcctattca ctttacaaag caacgactct
     1681 caaaacatgg gcagacaagt gtttcgcaga cctcgtcaaa tgatgaatcg acaaaccaga
     1741 agatttcaac gcagtatgag taagattttt cgtggaaaag cagggtcaac gaagcaaaag
     1801 acaatgacaa caaagcagaa tcaaaccaat aaagctaacc atactcgtca aaatgaacgg
     1861 actacaccaa agaaagaaac agcactttcc aatgtgaaaa agcaacaagg cggtatcggg
     1921 caacgattag gtgaaaagac agcccatgta ttggacacga aaaatcgtct agtcaataaa
     1981 agaaagcagg caaaacaaca gatacaggcg acaccaaccc atgtcaaata tgcccttcat
     2041 caaggaaaag agaatgtcac tcataatgta acagacttta aacaatctat ggcagatacc
     2101 aaagctcaac gcaaacaaca gcgtatgaac cagcaggagc aaaaacgaaa gaacattgct
     2161 caaaagcgtt tggagctgga taaagcaaaa gaaaaaagac gaaaagaaaa cccatcatct
     2221 cctgttcaga ataaccagca gcatgttcgc aagcgtcagc aaaccgtcac gcctgttttg
     2281 tcacaaaaca ctaaacgtga aaatcatcag cgaccattga aaaaagagca aaaacaaaag
     2341 acaccaccga agcaattcaa tcagaggaag ctaaataaaa gaacggataa gcgaggacaa
     2401 caaacatgaa gctaaaaata acagtgattt gtagtgtggt cttctctctc ttcttttttc
     2461 tcttattatt cctagtcatt ttctttgcag atgatacgga cagtggcgaa aacaacaagg
     2521 attcttctat tccacaaggc ggtgtgaccg tttcaccaga agtgttggca caccgtccgt
     2581 tgattgaaaa atatggtaag gaatatggga ttgaagacta tgtttcttat atcttagcca
     2641 ttatgcaagt ggaatcgggc ggtacagcag aagatgttat gcaaagttcc gaatctttgg
     2701 gtttaccgcc caacagtttg agtaccgaag agtccatcaa acaaggggtt aaatatttca
     2761 gtgaattatt aaccagtgca gaacaacaag gagtggatat agattctgtt atccaatctt
     2821 ataattatgg cggtggcttt ttaaattatg tgaggagtca tgggaaaaaa tatacctatg
     2881 aattagccga acagttttct aaagagaagt cgggtggtca aaaagcagac tatcccaatc
     2941 ctatagccat acctgtgaat ggtggttggc gatataacta tggaaatcag ttttatgttc
     3001 agttggtttc acaataccta accgacactt ccccaacaga atttgatgat gaaaccgtgc
     3061 aagtcattat ggacgaagcc ttgaaatacg aagggtttcc ctatgtcttt ggaggggcgt
     3121 cgcctaccac ctcttttgat tgtagtggct taatacaatg ggtgtatgac aaagctggca
     3181 tttctctccc tcgtgtcgcc caagatcagt atgacgctac gcaagaaatc tctatggaag
     3241 aagcccaagc aggcgacctt atcttctttc attcaaccta taatgcaggc acatatgtga
     3301 ctcatgtggc gatatattta gagggaaatc gcttttatca tgcaggcgac ccaatcggtt
     3361 atggcgactt aagcagtcgt tattggcaag accatctgat tggggctaga cgtgttattc
     3421 ataactaaga aaggaatgaa tcaaatgatt ataaaaatcg agcgaaaaga aaagaaggag
     3481 aaaccaaaga aatctaaaaa accgagcaag caaagaaaag ttccgatgat taaagttggc
     3541 acgcataaga aattaacctt tgttttatgg gttttattga ttggtagtgt cggatttgga
     3601 atctataaaa attttactgc cattgacact catactgtcc gtgaaacaga aatcattaaa
     3661 cagcagattg tcgataccaa ccaagtagaa agttttgtga aatcctttgc gaaagactac
     3721 ttttcgtggc aacaatccca agaagccatt gataaacgaa atgaaaaatt gacccattat
     3781 ttgacggaag aattgcaggt attaaatgaa gaaatgattc gtaaggatat tcctactagt
     3841 tctagtgtga acgatataca ggtgtggcag gtttctcaag tgaatgagaa cacctttgag
     3901 gtactgtttt ctgttgaaca agttattacc gaagacaaag acaaggaaac tatatcgtct
     3961 agctttcatg tggttgtcca tatagatgaa tcagataata tggttattat caaaaatccg
     4021 acaatgagta agaaacctca aaaatcggac taccaaccca aacagctaga aagtgaccat
     4081 acagtagata cagaaacgat ggacgaaatc actagctttt tagaaacgtt cttccagctc
     4141 tatcctaccg ccacagaaaa agaattgact tactatgtaa gtaatcatgt gttacctatg
     4201 attaacaagg aatatgtttt cgaggaattg gtgaatccta tctttaccag aaaagataac
     4261 caagtaatcg tgaatgtcgc tgtgaaatat ttagatcaag aaacaaaggc aacccaaatc
     4321 tcgcaatttg agttgatatt agaaaaacaa gataattgga agattgtaaa ataacaaata
     4381 ttggtacatg attacagata ctttgtaatc atgtactctt tttgataaaa aattggagat
     4441 tcctttacaa atatgctctt acgtgctatt atttaagtga ctatttaaaa ggagttaata
     4501 aatatgcggc aaggtattct taaataaact gtcaatttga tagcgggaac aaataattag
     4561 atgtcctttt ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt
     4621 gattctaaag tatccagaga atatctgtat gctttgtata cctatggtta tgcataaaaa
     4681 tcccagtgat aaaagtattt atcactggga tttttatgcc cttttgggtt tttgaatgga
     4741 ggaaaatcac atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac
     4801 taccttaaca gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga
     4861 caaaggtaca acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca
     4921 gacaggaata acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg
     4981 acatatggat ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct
     5041 actgatttct gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag
     5101 gaaaatgggg attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt
     5161 atcaacggtt tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa
     5221 ggtagaactg tatcctaata tgtgtgtgac gaactttacc gaatctgaac aatgggatac
     5281 ggtaatagag ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc
     5341 attggaactc gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta
     5401 tcatggaagt gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa
     5461 attttattca tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga
     5521 atatacaaaa aaaagacaac gtcttgcata ttaagtagtt cagttttgga gtacaaaaag
     5581 tgaaaatgta aacctaaatg agtatcaaat tgcaactgaa tttgatactc gttttttatt
     5641 gttaaatcaa cgtttgttgt taatttacac cacaaaatgt aagttaaaat gtatataaaa
     5701 atgcgaacgt gaaataatta ttgtaggaat gcaaattggt gtgttaaaat ggttacaatt
     5761 tactaagcaa aaaggggtgc attttcctat gcaatttctt gagccaaaaa ataagaacgc
     5821 taagtctgtg gattgggaaa tatcagagca agtaagggtg atagttaagc aatacgctga
     5881 atatgcagag cgtactgaaa gcgaagcagt ggatgaattc ttattgaaca ttttagacga
     5941 taagaagttt attgaatgga ttgccaacaa gcgaagtaat aagcggattg ttgaaaaaat
     6001 gggtatcaaa gatagagtgg gttgatagac aatgaccaag cttaaaagat tagccacgaa
     6061 ggaagatgaa attgtagatg ttaaaattcc gatttcaaac gaagaattaa aagatagggc
     6121 taaacaatat gatttattaa ctccaaaacg atttgcaaca aggtataaca aaatgttgtt
     6181 cttgccaacg tcttttaaat ggaacggttc tgaatatccc attcaatata attactgcat
     6241 caatccgttt tgttgtaatt ttgggaaaga acagcacaaa ttcaaagatg taaaagggaa
     6301 accaagtcga tataaaatga ctggtagttc caaagataaa ggtcataaag gtatgtactg
     6361 caatgacaat cctattggca gaggtgtatc gcaaaactgt acagtaaccc ctttatctaa
     6421 ttggtctgtt gttgaagaaa ttaaacgcct tatagaaatc aatagcatcc aagatgttga
     6481 gccagattat caatttcata aagaaggctg ctcggaagaa gaatcaaccc cattcaatga
     6541 gccgaagcaa ttttacaaaa gggggaaaag taggggcaaa tctcaacgct atcaatgtaa
     6601 ggcgtgtaag aagttcacta atgttctacc aaaaagagaa gaaaccacta cttatcacca
     6661 acaaaagaac accattcttc caatgttcgc taagatggtt gttggaaggg tttcagtcag
     6721 tcgtacttgt gatattttag gaattggagt tggaacctac tatcataagc tggagtggct
     6781 gtataggcgt tgtttggagt ttttagaacg atatgagaca caaccccttc aaacaaagaa
     6841 atttaacgag atgtggctta atacggacaa gatgcactac tacctaaata acgtccgtaa
     6901 gaaaggacaa ggttcaaaga aatatacggg gtttgaagac cttaatatgc aaacgtatat
     6961 agttgtatct gctgaggtct tatcaagata tgtgttccgt tctgatgttg cctatgattg
     7021 gaatatttca atggatgaat taaatgaaga tacaagaaaa ttcaaagaag accatctcaa
     7081 tacttttagt cgtaaaaatg ataggttaga ttggtcttat tatccacaag aaccatccgc
     7141 taacgatagc gaaaatagga atgcttatct tcacgaatta gggaaaatta ctaataggag
     7201 tagatttgtc gatggtttaa atgttgatgc tccttatact acaactgctc actattggtt
     7261 aattaaacaa atggttaatg ccgacgaatg gcgaatgatt agtgatgatg acttctctat
     7321 tagaaacgcc ttttatagag tattcacgaa agaattaagg ttgagcgatg cacatcattt
     7381 tatttgccaa gttaacaaga cgaaaagcag aaaacaatgc ttaaaagaat tcggacaagc
     7441 aaaagcagaa ctgctcgatt ggggagatat cagaggattc aaaacaaagt ttctacggac
     7501 tttggcatct cattatctaa ctgaattact aacaagtcat caattccacg aagaggctat
     7561 tagtaaagat ggagaaagat atcgcaaata tgccgataac cctatcaaac atccattagc
     7621 cactaaggat aaaggatttt attcagttga ttgtagaacg gatttatctg ctctggaacc
     7681 aaacgaaatc gcaaagatgc tattaaatgt taatgaccat tcgaccaata gctttattca
     7741 acagataaga cgttatatat catctcttga aagaccatta acaacagctc gtggtgataa
     7801 gaagagctat atttacgcta acttcaatcc gaagtatgct caatttgcga ttacaattct
     7861 aagaacttac tacaactttt gtcgcccttt taaatcagcg gataaaaagg ttttgacacc
     7921 tgctcaaaga ttagggatta cagataaaca atttgattgg aaagatataa tttatttcaa
     7981 atgacaaaaa gagcttggag taatcccaag ctcttgtgct ttatctttac tgttcttctt
     8041 caattaacgc acccgttaac tcaataagtt ttttctttca tatacatgaa aatagtagtt
     8101 atacggattt ttatcattct ttatcccttt ttgggcaaat acctcattcc attcctcata
     8161 attcacttct ggaaaaaaag tatctccttc gaattcatga tgtatttttg tgatgtacat
     8221 tttctcaaca taagggaaaa acaaattata aatctgttct cctccgaaaa taaaaatttc
     8281 ttcttcgttt ttacataact caaaaacatc ttctattgaa tgaacaattt cacaaccatt
     8341 aaaggtaaac cccttatctc tcgtcagaat aatatttctt ctgtcaggta aggctcttcc
     8401 gattgattca aggttcttcc tacctaatat tatcggatgt ccctttgtag tatttttaac
     8461 atattcccag tccttgggaa tcctccaagg aatgtcattc tctttgccaa tcactctatt
     8521 cttatccatc gcagcaatca aagaaacttt catttataac acagcctttt taactaattt
     8581 accatctaat ttcttatttc tatcaaaaaa ccttttctca ttatcctgcc cgttagttga
     8641 ataagcaatt ttttctataa gttatattat cataatacca caaaatcaca aattttcatt
     8701 caattatcat tttcaattgc attttataaa tcattttgta tgtaatttgg tatgtaagtt
     8761 aaaatgaaaa aagtagtcca ttttgaagta cattttgtca ttttgtactc caaaactgaa
     8821 ctacttactt gcatatatac gcctttatag tggagtacta catttacgag attcggttag
     8881 agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt
     8941 atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa
     9001 gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc
     9061 gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct
     9121 tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac
     9181 gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact
     9241 gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga
     9301 gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg
     9361 ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag
     9421 ctcggtttct cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg
     9481 ctatggttgc gaacaaggat tatatggttg gaatgtgacg gattgtaaaa tctgttttaa
     9541 gtacggttta tactatagcc ctgttagtac tccagcagat tttcggatgc ttactcctat
     9601 tgtactggag caagccttta gaaaagctgg aacagaattg ttagagccat atcttagttt
     9661 taaagtttat gcaccacagg aatatctttc acgggcatat aacgatgctc ccaaatattg
     9721 tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc attattattg gagaaattcc
     9781 tgctcgatgt attcaagatt atcgcaatga tttaactttt tttacaaatg ggcttagtgt
     9841 ttgtttagca gagctaaaag gatatcaggt taccactggc gaacctgttt gccagacccg
     9901 tcgtctaaat agtcggatag ataaagtaag atatatgttc aataaaataa cttagtgcgt
     9961 tttatgttgt tatataaata tggtttctta ttaaataaga tgaaatattc tttaatatag
    10021 atttgaatta aagtggaaag gaggagattg ttattataaa ctacaagtgg atattgtgtc
    10081 ctatttgtgg aaataaaaca agactacgaa tacgagtgga tactatactt aaaaatttcc
    10141 ctttatactg ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa
    10201 taacaatcaa agagccagac gccaagacgc agagccgata atttgagaaa tgaaactctc
    10261 atcttatcgg ctctttttgt ttatctgaat tttactgact agccttcaat atttcttgct
    10321 tcattgatac cttttgctag tgcttccatt aaggatagtt ctttgtctgt aaagctatcc
    10381 atgtattttt ctacctgtaa ccttcgagtg cttttaatca aattatctgt aggtaagaaa
    10441 aattcatcaa tagatacatg gagtaatgat acaagatcat acagcacttg tgtacttggg
    10501 tgttgtcctt tgttttctat atttgttaaa taacgtgggt caatttcaat cattgttccc
    10561 acttgttcac gagtgagacc ccgtttcatt cgagcctctt taatcgctaa accaacggct
    10621 ctaaaatcgt atttatcttc ttttttacgc atactaaatc acctctatac attttactgt
    10681 tcctatataa ccataaacag gtatagaaaa acatgttata tagtttatga gttcctatag
    10741 aacactgtgg aatttattaa tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt
    10801 atttacctat acaactttat aaaatttagt catactccta gaaagtagat aaaattggcg
    10861 acttaatgga gggaaaagtc gccatgagaa agataatctg tcatctagtt ctcttaatta
    10921 agttcttatc aaaaaaactt atccacctgt actggataga tatgaccatc aatgtggttt
    10981 tgcagattac atagatagaa aaatcaaagc atatgaacag agaaaacaat ctgtttatgt
    11041 gcttttttgg tcacttaata aatatctttt aggatttctc ttcatttatg caacaaatcc
    11101 cccgttcgtg tcggggttta gtatgaaggg agataaagaa gcacatgcca ctccctttca
    11161 cgacagaaag ggggtgaaaa gcatgaaacc atcttctttt caagaaacca tagaaaatca
    11221 gtttgactat atctgcaaac aagttattga agatgaacga aaagattact tcaaacatct
    11281 agacagacta tcagcaaaag aagtctcgtt ttctgatata gataactacc ttgtcaactc
    11341 tttttctact gttgatacgt acgtcactga tttccaacta tttaaattat ttggattaag
    11401 aatcaatgtt gaaagtgatt tattaagtga agccctgcgt catctttcag agaaaaaacg
    11461 gaacattatt ttacttcatt actttatgga tatgagtgat gtggagattg cggagttact
    11521 aaatttgaat cgttcgactg tttatcgtca tcgaataagt ggactagcca tgattaaaga
    11581 attcatgaag gagtgtaaag aatgaaaaca gcttatccaa gg
//
LOCUS       DACCSK010000270         8429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.107, whole genome shotgun sequence.
ACCESSION   DACCSK010000270 DACCSK010000000
VERSION     DACCSK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.107"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(271..507)
                     /locus_tag="IVP39_002900"
     CDS             complement(271..507)
                     /locus_tag="IVP39_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7587927.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(690..1061)
                     /locus_tag="IVP39_002901"
     CDS             complement(690..1061)
                     /locus_tag="IVP39_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587928.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1058..2386)
                     /locus_tag="IVP39_002902"
     CDS             complement(1058..2386)
                     /locus_tag="IVP39_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7587929.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2347..2676)
                     /locus_tag="IVP39_002903"
     CDS             complement(2347..2676)
                     /locus_tag="IVP39_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7587930.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2934..3305)
                     /locus_tag="IVP39_002904"
     CDS             complement(2934..3305)
                     /locus_tag="IVP39_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7587931.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            3450..3635
                     /locus_tag="IVP39_002905"
     CDS             3450..3635
                     /locus_tag="IVP39_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7587932.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            4147..4365
                     /locus_tag="IVP39_002906"
     CDS             4147..4365
                     /locus_tag="IVP39_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587933.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            4578..5237
                     /locus_tag="IVP39_002907"
     CDS             4578..5237
                     /locus_tag="IVP39_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7587934.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5240..6583
                     /gene="vanS"
                     /locus_tag="IVP39_002908"
     CDS             5240..6583
                     /gene="vanS"
                     /locus_tag="IVP39_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7587935.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            6754..7561
                     /locus_tag="IVP39_002909"
                     /pseudo
     CDS             6754..7561
                     /locus_tag="IVP39_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
     gene            7579..8415
                     /gene="vanW"
                     /locus_tag="IVP39_002910"
                     /pseudo
     CDS             7579..8415
                     /gene="vanW"
                     /locus_tag="IVP39_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         2194 a         1947 c         2096 g         2192 t
ORIGIN      
        1 tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt cctacgcatt tagaacccgg
       61 tattgtcggg gataggatga agtattcgct tgcccctgca aacatgcggg agaagccttg
      121 cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag ctataattag acccctgaaa
      181 cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag
      241 cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt tccagcggga agctcggcaa
      301 tgcttccagc agtttcaggg aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt
      361 tttgccgttt gcctgtctga tctccaccgt ggaaagctcg ttgatgtagt cagcataatg
      421 ggccaacact ttttccgtgg cccacctctc accggcaacg gcggctttga tcgtttcata
      481 gtccagcagc ttttcttgtc cgtccatcct gatacccccg tcagactgat tttttctatg
      541 ataccatatt cggaagcatg gaaccagaga aaacggcgct tttctgctgg taaattctac
      601 gcatttagaa cagcacttcg gcggggatag gataaaacac ccacataccc cgctgaccat
      661 gccggaaaag ccttgaaata taagggcttt tacagctcta agtgcgtagg tttccagtat
      721 cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag
      781 tatttggccc ggttagactt cggcgtgaac gtggccccgc actcacagca gcgtttcact
      841 tcgtcccggt ctttggtgat ctcggcatac agggccgcat ccagcgggag gacagcggcc
      901 ttaaaccagc ggcacagaag cgaataggaa atgctctgta cgcaaataca ttcttcgccg
      961 tcgtccagca acaggcagtt gccgtcacaa tagttgcagc actcatgcgt cagcttccgg
     1021 gccttgcgat attgagggta attcattcgt ggaatactca tagctcttgc gtccattcct
     1081 ttcccggcgt catacgcaaa atgccgtcca cattctgctt gatgatgccg tattcctgca
     1141 tctgcttttt cgcttcgccg tacttctcat acagccgttc cttttcctcg gccaatcgct
     1201 tgtactcggc tttcagggcg tacagatcgg ggagcttttt cacaccctgt ccctttaggg
     1261 cctttgccgc agcttcatac aaaatcaacg tgctttcatg ctcacgccgg aattttgcct
     1321 tatccgctgc attgcggtat tccatagcca cgggccagag ctgcttgtag gtagaaatat
     1381 tctttatcag caccgccata tccgcaagcc ggtgttccat agctttgagg gtagcggcgg
     1441 cctcgtcgct ggcggcggtg atctcggcca ctttgctttc caaatccggg taggtgtcta
     1501 tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg
     1561 cccacctctc atagcctgcc gactgctggg ccttaatgtt gttttccaga tcaatccgca
     1621 aagaaatccc cttgcgctct ttgggcgctc tggtgataac gcggcctttg atacgctctt
     1681 tgatggcttc ctcggtgtag gcttccccca atgtcttgca gcgggtaaag cgttcctgtc
     1741 cgggagcgcg gaaagaaata aactttcccg gcttgatctc atagccctgc gcctgcatga
     1801 gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc ggcgtcaatg gctgctttga
     1861 gcttcgcttt ccagctcgtc cctttccgct gggcgtccca ctcggcatag ctcttgccct
     1921 tactctggcc gggcttcacc acggacaatc cgttttcctt gcacagcctg tcgctggtgc
     1981 gccggatata ggcatagctc tgtttgttgg aaatgtactt gcgctgattt accatatcca
     2041 cggcacagaa aataatgtga ttgtggacgt ggcccttatc aatatgggta gtcagcacat
     2101 agggatattt cccttgcagc acttcctcgg caagctgctt cccgatctca tgggcctgtt
     2161 ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg gtggcccaga ttattgccct
     2221 tttggtatgc ctgcgccagc agcatttcaa attcaatgtc cgctgtttca taagagcagg
     2281 caaaggaggc caccagcagc ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg
     2341 cttttttcag cgttgacttg ataggatgta tctttgtaac tgccatatct gcgccagcgc
     2401 cccctttata tcctcgatgt cctgtgcgta caccggcccc ataccgttca gcctctttgc
     2461 aatctgattg acgttgacgc cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct
     2521 ggtgtccatg ttgacgatat agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt
     2581 cttcgtatgc agcagcgcca ttttctgttc aatcatagcc cgttcctccg gcgtcacagg
     2641 gacgcgcagc acgatttttc gctttctgtt tgccatgctt caccgtcctt tccattcagg
     2701 ggtttgggga tttccccaac aagcaaaacg gacgcggccc ggcagggacg gggcaggccg
     2761 tttttccggg agtggctaca ctccctatgc ttgctacgtt actgtttctc cactaacaag
     2821 acgtttccaa agccccaaac tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa
     2881 aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa cagaggatgc cagttacaca
     2941 tcctccgttt cattcttctt cggcttttca attagtatgg cgctgtcaat cgcgccctcg
     3001 atgatcggca ggtattcttc ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt
     3061 tcgctttcct cccgcatgat ctccgggtta aagtagcgtt ccacaggaag cccgcaggct
     3121 ttaataagct gtatgacaac cggcagactg ggaatggccc cgtcgttttc gatgttggca
     3181 agatagcgcc agtcaatatt tattttctct gccattgctt tgcgcgaaac gcgctttgct
     3241 tgccgggccg ctttcacatc cgcgccgaaa gtttcaaagc cgggacaatc ttcaatattc
     3301 gccatatcgc acctcccact tacattgtat atttcatact ctgcccgtgg aatgttggta
     3361 tatgcacctt tcttaaagtt tatatttcac attttgtttc ttttattctt cgagcaaaag
     3421 aattataatg taccctgtgg aggtgtacta tggactacat gacgttgaaa gaggcaggcg
     3481 agaaatgggg tgtgccccca cgatggataa actactattg tgctgcggga cgcatccccg
     3541 gcgctgtaaa gatggcgacg atctggctca ttcccaaaac tgcggaaaag ccgattgaca
     3601 ggcgtacaaa gaaaggaaag gcggttactg aatagtataa tgaaaccggc gcattttgga
     3661 tatggttttg aagagagaga aagcatggta ttttgtcacg gaaaaactat aagatgatag
     3721 atggcaggct gctccagacc gataaaaagt attctcatct gaaacagaaa gaaaagattt
     3781 acacatggat gtatgaagaa gcgaaacttt atcatgacag aaatggaaaa tgcccgaaac
     3841 aaagacgagg aagctgctgc tgtatcagcc gtatatggta gaattgaaaa ggccggcatc
     3901 tggattcttt atggtgaagt ctccagacat tggataactg ctggtctaat gcgcctttga
     3961 gtatggggtg attgatttta ataccgggga ggtacggctt tttgaggaaa atattctggg
     4021 atttacattt ggcgattatt acatttcctg taactgcaag gacacggatg atgaagatat
     4081 gaggaatata gacttgctga ttttctactg tcttgaacgg tattaggact gaatctgctg
     4141 cgcggagtgc ggcaagagcg accggaggga gtacacacag gccctgcctg tgtgaaaagc
     4201 cataccgcaa ggtgcattcg caaaaaagag gccgcgcatt ttgctgaggc ttggggtgtc
     4261 cccaacaagc aaaacgagaa cgccgcaggc gggcaagtgg tggatcagaa tatccgtaaa
     4321 ggatattctg atccattgct tgccagtatt atcgggatgt tttgacaacc gctgtaatcg
     4381 aaacatccca aacattaaga aggtgttaat tattattatc ttgatttaat tcgtttaaac
     4441 caataatata gatacataca ccatgaaaag aagcgagtat cccaatgacg gatgattaaa
     4501 atccgaaaag aaatcgcttt gaaactacag ggaaactaca gactgttatg ttatcttctt
     4561 aaaatggagg gatttttatg tcgatacgaa ttctacttgt cgaggatgat gatcatatct
     4621 gtaatacagt aagggcgttt ctggctgagg caggatatca ggtggatgcc tgcacagatg
     4681 gaaatgaggc atacaccaag ttttacgaaa acacttatca actggttatt cttgatatta
     4741 tgctgcccgg tatgaacggg catgaacttt tgcgtgaatt tcgtgcgaaa aatgatactc
     4801 ccattctgat gatgacagcc ctgtcggatg acgaaaacca aatccgggcg tttgatgcag
     4861 aggcagacga ctatgtaaca aagccattca agatgcagat tttactaaag cgggtggaag
     4921 ccctgctgcg gcgcagcggt gcgctggcaa aggaaatccg tgtcggcagg ctgacacttc
     4981 tgccggagga tttcacggta ctttgtgacg gtacagagct gcccctgaca cgaaaagaat
     5041 ttgaaatcct tttgctgctg gtgcagaaca aaggcagaac cttaacgcat gaaatcattt
     5101 tgtcacgtat atggggatat gactttgaga gtgatggcag cacagtccac actcatatca
     5161 aaaatttgcg ggccaagctg ccggaaaata tcatcaaaac cattcgcggt gtaggatacc
     5221 gattggagga atcataataa tggaaagaaa agggattttc attaaggttt tttcctatac
     5281 gatcattgtc ctgctactgc ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt
     5341 gtcttatttc agagtgatgg aattacagca aacagtaaaa tcctatcagc cattggtgga
     5401 actgattcag aatagcgata ggcttgatat tcaagaggtg gcagggctgt ttcactacaa
     5461 taaccaatcc tttgagtttt atattgaaga taaagaggga agcgtactct atgccacacc
     5521 aaatgccaat acatcaaata gttttagacc cgactttctt tatgtggtac atagagatga
     5581 taatatttcg attgttgctc aaagcaaggc aggtgtggga ttgctttatc aagggctgac
     5641 aattcgggga attgttatga ttgcgataat ggttgtattc agccttttat gcgcgtatat
     5701 ctttgcgcgg caaatgacaa cgccgatcaa agccttagcg gacagtgcga ataaaatggc
     5761 aaacctgaaa gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg cactggctca
     5821 tgatatgcat tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat
     5881 cgcaagggaa catgagttgg aggaaacaca gcgatatttc tttgcggcag cttctcatga
     5941 gctaaaaacg cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg aaaatatcgg
     6001 tgactacaaa gatcattcta agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg
     6061 caaaatcatt tccgaaatac tggagcttgt cagcctgaat gatgggagaa tcgtacccat
     6121 agctgaaccg ttggacatag ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt
     6181 ggcagaggca aacaaccagc ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc
     6241 cgatccgagg ctgctccaaa aggcactatc caatgtcata ttgaatgcag ttcagaacac
     6301 gccgcaggga ggcgaggtac ggatatggag tgagcctggt gctgaaaaat gccgcctttt
     6361 tgttttgaac atgggcgttc acattgatga tactgcgctt ccaaggctgt tcaccccatt
     6421 ctatcgcatt gatcaggcgc gaagcagaaa aagtgggcga aacggtttag gacttgccat
     6481 cgtacaaaaa acgctggatg ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg
     6541 cgttttgttc tggctggatt taccgctcac atcaacattg taaatattta aaacttaaat
     6601 gattttgacc gacaggtata accctgccgg tctttttttg ctgctacagg aaaactacag
     6661 actggctaca gggaaagtac agatgcgctt gccataataa caatcgtacc agccacaaat
     6721 cgtagtttta ttgcaaagga ggcattcgat caaatggaaa aaagcaacta tcattccaat
     6781 gcggatcatc acaaacggca tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg
     6841 ttcattattt cgttcacagt ctgcacgctg tttttggggg tggagactgg cttccgtatt
     6901 ggaggcaaca cagataccgc ccatccctgc aactcataca ggcagcagca ctgacgtagt
     6961 ggagaatttg gaggaaaacg ctcttgccac cgccaaagaa cagggagatg aacaggaatg
     7021 gagcctgatt ttagtgaaca ggcagaaccc catccccgca cagtacgatg tggagcttga
     7081 gcaactatca aatggtgagc ggatagatat tcggatttct ccctatcttc aagatttgtt
     7141 tgatgccgca agaactgatg gagtttaccc gattgtcgca tccggatacc gaacaacaga
     7201 aaaacagcaa gaaattatgg atgaaaaaat tgccgaatat aaggcgaaag gctacacctc
     7261 tgcacaggct aaagcggaag cagaaacttg ggtggccgtg ccgggaacga gcgagcatca
     7321 gcttggtctt gctgtggata tcaatgcgga cggaattcat tcaacaggca acgaggttta
     7381 tagatggctg gatgaaaaca gctatcgttt tggttttatt cgtcgctacc caccagacaa
     7441 gacagagata accggtgtga gcaacgagcc gtggcattac cgatatgttg gcatcgaagc
     7501 tgccacagag atgtacaacc aaggggtttg ccttgaggaa tatttaaaac cagaaaaatg
     7561 agaaaaggat gttatgttat ggacagaaaa agattgacac agcgtttccc gttcctgctt
     7621 ccaatgagac gggcgcagag aaaaatgtgc ttttatgcgg gaatgagatt tgacggctgt
     7681 cgctatgcgc agacgatagg ggaaaaatcg ctttcccatt tgctctttga aacggattgc
     7741 gcattataca accacaatac cggatttgac atgatatacc aggaaaacaa ggtgttcaac
     7801 ttaaagctgg cagcaaagac cttaaacggc ttattgataa gaccggggga aaccttttct
     7861 ttctggtggc tggtacgcca tgcggacaaa gataccccct ataaagacgg ccttacggtg
     7921 accaatggta aactcaccac catgtcgggc ggcggtatgt gccagatgag caatttacta
     7981 ttttggatgt tcctgcatac gccattgaca attatccagc gcagaggtca cgaagtaaag
     8041 gagtttccag agccaaacag tgacgagatt aaaggggtgg atgcaaccat ttcagagggc
     8101 tggattgatt taaaagtgcg aaacgatacc gactgcacct accaaatatg agtgacctta
     8161 gatgatgaga aaatcatcgg tcaggtgtcc gccgacaaag agccccaagc attatacaaa
     8221 attacaaatg gcagtatcca gtatgtccgt gaaagtggcg ggatttatga atatgcccag
     8281 gttaaacgga tgcaagttgc cttaggtacc ggggaaataa tagattgcaa gctgctttat
     8341 acaaacaaat gcaaaatctg ttatcccctc ccggaaagtg tggatattca gtctaatggt
     8401 gataagtcaa gatgaaattt aagattttc
//
LOCUS       DACCSK010000271         4990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.156, whole genome shotgun sequence.
ACCESSION   DACCSK010000271 DACCSK010000000
VERSION     DACCSK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.156"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            40..1461
                     /gene="lsa"
                     /locus_tag="IVP39_002911"
                     /pseudo
     CDS             40..1461
                     /gene="lsa"
                     /locus_tag="IVP39_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
     gene            1528..2055
                     /locus_tag="IVP39_002912"
     CDS             1528..2055
                     /locus_tag="IVP39_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7587936.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2266..3327
                     /locus_tag="IVP39_002913"
     CDS             2266..3327
                     /locus_tag="IVP39_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7587937.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3330..4226
                     /gene="murQ"
                     /locus_tag="IVP39_002914"
     CDS             3330..4226
                     /gene="murQ"
                     /locus_tag="IVP39_002914"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7587938.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4240..>4990
                     /locus_tag="IVP39_002915"
     CDS             4240..>4990
                     /locus_tag="IVP39_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HAP7587939.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGV"
BASE COUNT         1738 a          861 c         1120 g         1271 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacctt ttgacacaca atggaaacta
       61 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa
      121 ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca
      181 aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg
      241 gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc
      301 agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac
      361 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa
      421 gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga
      481 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat
      541 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct
      601 caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc
      661 gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact
      721 gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga
      781 tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa
      841 gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt
      901 ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt
      961 acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc
     1021 attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca
     1081 aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg
     1141 gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt
     1201 gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa
     1261 aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca
     1321 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa
     1381 cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt
     1441 atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg
     1501 aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     1561 tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag
     1621 atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     1681 atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc
     1741 ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg
     1801 ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat
     1861 ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa
     1921 gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct
     1981 tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg
     2041 gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt
     2101 ttgcttagca gtgccttttt taggttatga caattctttt gataatataa actcaacctt
     2161 tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata
     2221 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     2281 atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     2341 aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat
     2401 caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc
     2461 gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg
     2521 aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc
     2581 gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc
     2641 gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca taattattat
     2701 ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa
     2761 attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta
     2821 tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta
     2881 gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac
     2941 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     3001 gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca agacgatgca
     3061 agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga
     3121 aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat
     3181 atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     3241 gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat
     3301 caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa
     3361 cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc
     3421 aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta
     3481 tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata
     3541 tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat
     3601 tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg
     3661 tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga
     3721 atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga
     3781 tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata
     3841 aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat
     3901 ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga
     3961 tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca
     4021 aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag
     4081 ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta
     4141 tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa
     4201 acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact
     4261 ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc
     4321 attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc
     4381 tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc
     4441 atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg
     4501 aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga
     4561 aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa
     4621 ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct
     4681 gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca
     4741 gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa
     4801 aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt
     4861 gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg
     4921 gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc
     4981 ctaggtgtca
//
LOCUS       DACCSK010000272         3971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.176, whole genome shotgun sequence.
ACCESSION   DACCSK010000272 DACCSK010000000
VERSION     DACCSK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.176"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            273..1757
                     /gene="lsa"
                     /locus_tag="IVP39_002916"
     CDS             273..1757
                     /gene="lsa"
                     /locus_tag="IVP39_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7587940.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            1811..2614
                     /gene="lnu(B)"
                     /locus_tag="IVP39_002917"
     CDS             1811..2614
                     /gene="lnu(B)"
                     /locus_tag="IVP39_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAP7587941.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            3302..3971
                     /locus_tag="IVP39_002918"
                     /pseudo
     CDS             3302..3971
                     /locus_tag="IVP39_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1381 a          572 c          786 g         1232 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca ccttatgtta tgtataacaa
       61 aaggtaataa tagtatttgt ttttgagccg taagaattta ctttcttgcg gcttttaata
      121 tttgtaaaag aaaggaagat aaacgtgata gacaatgata ctatgtcatt catgacaaaa
      181 tccttgcaga gttggataac attatagagt aattaatgcc gaaaaccgac tttgcttata
      241 aaatttttat aatgtaaagg aggaagaaat atatgtcctt aataaatgtt tcaaatctaa
      301 ctttttcata tgaaggaagt tatgacaata tttttgaaaa tgtaagtttt cagatagata
      361 cagattggaa actcggtttt attggaagaa acggacgcgg taaaactact ttcttaaatt
      421 tactgcttgg caaatatgcg tattccggca atataagttc tacagttaag tttgagtatt
      481 ttccttatga tgtggaagat aagagtctat atacaattga agtaatgaag agtatttgta
      541 cggaatttat ggattgggag atttttcgtg aaatatcatt gcttgatgtt caagaagatg
      601 ctttatatcg tccgtttaat acattgtcaa atggtgagca aacgaaggtc cttcttgcag
      661 ctttattcct tacagcgagt tgtttcctgc ttattgatga acctacaaac catcttgaca
      721 tcgatgcacg taatgtagtg caaaactatt tgaaacgcaa gaaggggttt attttggtat
      781 ctcatgatag aagcttactt gatcaatgtg ttgaccatat actatctatc aataaaacga
      841 atatcgaaat ccaaaaggga aattttactt cttggtggga gaacaaaacg ttacaagata
      901 attttgaact ggcagaaaac aagaaactcc ttaaagaaat aggaaggttg tcttatgcag
      961 caaaacgtag ttcaaactgg tcaaataaag tagaaaaaag taaatatgga acaacaaatt
     1021 ctggttcaaa actggataag ggttatgttg gacataaggc tgcaaaagcg atgaaacgtg
     1081 ccaaaaatat tgagtcaaga catcaggaag ccgttttaca aaaatcagaa ctgctccaca
     1141 acattgaaca atatgatgac ttaaaaattt caccacttga atttcacaaa gagtgcttaa
     1201 tagaagcgaa tgatttatca ttgtcttatg gagataaaga agtatgcagt aatcttaatt
     1261 tcagagtcaa tattggtgat agagttgcca ttatcggaaa aaatgggagt ggtaagtcta
     1321 gtatcctaaa attgattaat ggagatgata ttaaatttac cggaaatttt atgctagcaa
     1381 gtggactaaa aatttcttat atttcgcaag atacttcata tttaaaaggt aatctatctg
     1441 aatttgccta taataataag atcgatgaaa ctctatttaa aacgattctt cgtaaactgg
     1501 attttaatag agagcagttt gataagaaca tggtggattt tagtgctggt cagaaaaaga
     1561 aagtactaat tgctaaaagc ctttgtgaaa gtgcacattt gtatatatgg gatgagccat
     1621 tgaactatat tgatattttt tcacgtatcc aaattgaaaa aatgattttg gaatattgtc
     1681 ctacactatt gtttgtggag catgatgatg ctttttgcaa taacatttgt acgaaaaata
     1741 ttaatttagg tttgtagaga ttttgagttg ccacaataac taaaagatat ttacatgaaa
     1801 gggtgaagaa atgttaaaac aaaaagaatt aattgcaaac gttaagaatc ttactgagtc
     1861 agatgaacga attacagctt gtatgatgta tggatcgttt accaaaggag aaggtgacca
     1921 atactctgat atagagttct atatattttt gaaagatagt ataacctcga actttgattc
     1981 atccaactgg ttgtttgacg tagctccgta cttgatgctt tataaaaatg agtacggaac
     2041 agaggtagtt atttttgata atcttatacg tggggaattt catttccttt ctgaaaaaga
     2101 tatgaacata atcccctcgt ttaaagattc aggttatatt cctgatacga aggctatgct
     2161 tatttacgat gaaacagggc aattagaaaa ttatttatca gagataagtg gtgcaagacc
     2221 aaatagactt actgaagaaa atgctaattt tttgttgtgt aatttctcta atctatggtt
     2281 gatgggaatc aacgttctaa aaagaggaga atatgctcgt tcattagaac tcttatcaca
     2341 acttcaaaaa aatacactac aacttatacg tatggcagaa aaaaatgctg ataattggct
     2401 aaacatgagt aaaaaccttg aaaaagaaat tagccttgaa aattataaaa aatttgcaaa
     2461 gaccactgct cgattagata aggtagaatt atttgaagcc tataaaaatt ctttgctatt
     2521 agttatggat ttgcaaagtc accttattga acaatacaac ttaaaagtta cacatgacat
     2581 tttagaaaga ttgttgaatt acattagtga ataggagagt aacgttattc acagtattgg
     2641 gtactgaaaa ttaacagcat acctcagttt ttcaaaaaac agcgaagcct atggatatgt
     2701 tcccgtctac cgatagtgca aaaagtgtcg tggatatgtt tccgagaacg gacatacaca
     2761 aatgacattc attctgtcct ctcaatccat gtggaagcag tatgtctgtt aacaagaaaa
     2821 taattattgt tcgtcctgac gagttgttag actcgaaaag gataaacatc atttttagaa
     2881 ccggtagccg atttttggct agcggttttt tgggctcttt ggcaaatagt ggtgattgaa
     2941 tctcgcgcta tcctatggaa aaccgccttg tgtgaggggc tgcgagaccg cttcgatcct
     3001 agaattgctg aagcacgaat ctagcgtcac tcacatttca tcttttgacg tagctgagac
     3061 aaggtgtttt tcttcgatcc gctagtggtt aatctctttt tggtgcaaat atggtttaga
     3121 atttctttgc atggcttaag ggagtggaga atcggatatt agtaggattt acatccacag
     3181 ataaaaatag gcttgatttt aacagcgaaa ctaaaatacc ctacttaaat gaagtgatag
     3241 attagatgtt gggttgttag gagatgctta aaaatcctat gcaatcaata gtttttagtg
     3301 tttgttgact tgatccctaa atcgcctgag tactaaacgt tgagtgtgtc tgcttactgt
     3361 cactagccag taagtgagac tgtagttaat ctctcatttt ccccagtatt atgttgaaaa
     3421 tagccatgaa gcgattattt ctaaagagtt atttttgcag gctcaagaag aaatttatcg
     3481 gagaagtaat atctacacag gagaaggcaa gaacaaaaga atttatagta gcaagtacgc
     3541 tttaagtgcc atcaccttct gtggatattg tggcgatatt tataggagaa cctattggaa
     3601 tattcatggc agaaaagaat ttgtctggcg atgcgtgact agaatcgagc agggtcccga
     3661 ggtatgtaag aaccgaaccg taaaagaaga tgaactctat ggtgcggtaa tgaccgcaat
     3721 taataagcta cttgctggtg gcaacaatat gataaagaca ctggaagaaa atattcatgc
     3781 tgtgattgga gaaaccacag aataccaaat ttcagagatt aataccctac tagatgaaaa
     3841 acaaaaagaa ctaatcaagc tggccaataa aggtcaagat tatgaatacc tggtagatga
     3901 gatcgatgag atgcgagata agcgtcagac tcttttagta gaagatgcct ccctcagtgg
     3961 cgagaacgag c
//
LOCUS       DACCSK010000273         3775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.183, whole genome shotgun sequence.
ACCESSION   DACCSK010000273 DACCSK010000000
VERSION     DACCSK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.183"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..137
                     /locus_tag="IVP39_002919"
     CDS             <1..137
                     /locus_tag="IVP39_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7587942.1"
                     /translation="QEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEWVED
                     "
     gene            149..1096
                     /locus_tag="IVP39_002920"
     CDS             149..1096
                     /locus_tag="IVP39_002920"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7587943.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1116..1643
                     /locus_tag="IVP39_002921"
     CDS             1116..1643
                     /locus_tag="IVP39_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7587944.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1706..2359)
                     /locus_tag="IVP39_002922"
     CDS             complement(1706..2359)
                     /locus_tag="IVP39_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7587945.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2491..3333
                     /locus_tag="IVP39_002923"
     CDS             2491..3333
                     /locus_tag="IVP39_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7587946.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3491..>3775
                     /locus_tag="IVP39_002924"
     CDS             3491..>3775
                     /locus_tag="IVP39_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP7587947.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETEN"
BASE COUNT         1342 a          676 c          776 g          981 t
ORIGIN      
        1 tccaagaaga gatgaatcat caaggagata atttcactcg attacaagaa ttgcaaaacg
       61 acgtatctga aacagaagca caacttgaag aaaaaatggc acggtgggaa tacttgagtg
      121 aatgggtaga agattaggag gaaaaaggat ggaacaagcg tatcttgact taggaagaaa
      181 attgttggaa gaagggcatc gtaaagaaga cagaacaggc acaggcacat taagcttatt
      241 cggataccaa atgcggtttg atctgcaaaa agggtttcct ttgttaacaa cgaaacgagt
      301 accttttgga ttgatcaaaa gcgaattgct ttggttttta aaaggagata cgaatatccg
      361 ctatctatta caaaataaaa accatatttg ggatgaatgg gcatttgaac gctacatcaa
      421 aagtccagac tataatggac cagatatgac tgattttggc catagatgtt tgacagatcc
      481 tgaattcaat gaagaatata aaaagcagag caagcttttt tgcgagaaga tcctaaccga
      541 tgattccttt gctgaaacat acggcgatct tggacacatc tacggctatc aatggcgtca
      601 ctgggaaaca aaagacggtg gctttatcga tcaaatcaaa caagtgatcg aagcaatcaa
      661 aaagactcct gattctagaa gattgatcgt ttctgcatgg aatccggaag atgttcctag
      721 catggctttg cctccttgtc atacgatgtt tcaattttat gttcaagagg gacggctaag
      781 ctgccaattg tatcaacgca gtggggatgt gtttctaggc gttccgttca acattgcaag
      841 ttatgcgttg ctgactcacc taattgcaca tgaaacaggt ttagcggttg gcgaatttgt
      901 tcacacatta ggagatgctc atctttatat caatcatatc gaacaaatga aagaacaatt
      961 gagccgagaa atacgcgcat ttccgacttt agttttgaat caggaaaagc agtcagtatt
     1021 tgattttgag atggaagata tccaattaga aggatatgac ccacatccgg cgatcaaagc
     1081 tcctatcgct gtttagaatc agaaaggatg agaaaatgtt agctgcaatt tgggcacagg
     1141 atgaaaacgg attgattgga aaagaagacc gtcttccttg gcgtttgcca aatgatctga
     1201 aattcttcaa acaaatgaca gaagcaaata cactagtgat gggacgaaaa acattcgaag
     1261 ggatgggtag ccgtccgttg ccgaatcgtc agacgattgt tttgacgaga gattcttcct
     1321 ataaagcaga aggcgtgcat gtcatgcata gtgtggaaga cgtgcttgct ctggaaaaag
     1381 aaacagacgg tatctttttc atcgctggag ggtcagctgt ttatcaagaa tttctgccat
     1441 tttgtacgat attataccgt acagtcatcc atcatgtatt cgatggagat gcttattttc
     1501 cacctgttga ctggtctgat tggtcactga tcaatactag tcaaggagaa atggatgaaa
     1561 aaaatcgtta tccgcatcaa tttgaaacgt atcagcgtaa tgagaatcct gtaaaaagcg
     1621 ggacaggcga attgacgata taaaaaagct atggatagac tagctgttat ttagagctgt
     1681 cccatccata gctttatttt ttttattaag taaagaataa aacagagaag tacataaaac
     1741 ctgcacctaa cataacaaac aaatgccaaa caacatgcat atagcggata gaattcaagc
     1801 tataaaagaa tgcgcctatc gtgtaagaaa gcccgcctgc taccatcaaa ataacaccag
     1861 tcattccaag agagtgataa agaggaacag cagccacgac acagagccac cccataaaaa
     1921 tatagatgat cgtagatact tttgatactc tctcccgttt atgcaacgtc aacgctttat
     1981 atataatgcc aagaatcgca aacaaccaga ttaatacaaa taaagtccaa cctagccatc
     2041 ctcggatact cagcaaacaa aaaggagtat aacttcctgc aatcaaaaga aaaatcgaac
     2101 tatgatcgaa tacttggaac accttttttg cttttgtaaa aatcaatgaa tgaaacaaag
     2161 tagacgttaa aaataaaaga atcatcatcg atccatagat agcataagag acaatatgga
     2221 gggaggaatg caagtgggca ccttttacta aaagaaccac tagtcccgcg atactcaatc
     2281 cagtcccaat tccatgggtg actgcattaa acacttcatt taatatttca tacgttcgat
     2341 taaatttcgt tttttccatg aaaacggctc ctttactagg tgtaattaat gaaaaatcat
     2401 tatttgtctg ttatactaaa aatagaaatt gtcttacatg tagtttaaca tgaataaggc
     2461 atcttgttaa agaaagagag agagaaaata atgacaaatg taaaaatcgt aacagattct
     2521 tcatgtacga tggaaaaaaa tgtacgcgat gctttaggca ttcgtgtcat gccgctatcc
     2581 atcatgatcg acggaacagt atatcccgat gatgaccaac tagacggcga aagatttatg
     2641 gaaatgatgg cgaaagcaaa aaatctgccc aaaacaagcc aaccaccaat tggagaattc
     2701 atctcgatgt atgatgagct aggagaagac ggcagtgaag tgatttcgat ccatatgaca
     2761 aaaggcttga gtggaacagt agaagcagca agacaagcca gtaatctaac aaaaacgaaa
     2821 gtaacggtta tcgacagtga taccactgac caaggattat cttttcaggt aattcgagca
     2881 gctgaattag caaaagaagg taaaacagta gagaaaatcc ttgaagaagt caaaaaagta
     2941 caagacaata caaaattata catcggaata tccacactag ataacttggt aaaaggtgga
     3001 agaatcagcc gaacaactgg tttgctgtct agtgttttga acatacgcgt ggtgatgaat
     3061 ttcgcccatt ctgaattaat ccctgtaact aaggggcgag gaaagaaaac gttcgataaa
     3121 tggtttaata atttgaaaaa tgaactgaaa gaattgccaa atgtgcgaca aatcggtatt
     3181 tcacatgcgg atgcaaaaga attggcgcaa gaattcaaag agggtttgca agaattattc
     3241 ccggacatgc atatccctgt actgcatacg aatccggtta tcgctacgca tactggtaaa
     3301 gacgcgtttg caatcatgta ttatgtagat tagtcgaaaa gaggataagc cctcagcctg
     3361 aaaggcttgc agcttattct ctttttcttt ataaaattag gaaaataatt gttttctttt
     3421 ttcataaaga ctaccttttt gataaaattt ttgataacat tagaaccaat cgaatacaag
     3481 aggtgaaaaa atgaaattaa cgaaacgaca tttgatcctc gttttgttcc cattattaat
     3541 tgcgatcatc atctatgcag gtttagctgc ttctataccg aaagctggcc cactaatgaa
     3601 accagtaact actgtgagag aagatccaaa taaaaaagaa aaacttcact acatagctat
     3661 cggagattcg ctgactgaag gagtaggaga tcaaaccaag caaggaggat tcgttcccct
     3721 tgtcgcaaat gatctaaaag accgttataa tttgaccgcg gttgaaacag aaaac
//
LOCUS       DACCSK010000274         3026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.193, whole genome shotgun sequence.
ACCESSION   DACCSK010000274 DACCSK010000000
VERSION     DACCSK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.193"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            47..1018
                     /locus_tag="IVP39_002925"
     CDS             47..1018
                     /locus_tag="IVP39_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7587948.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="IVP39_002926"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="IVP39_002926"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7587949.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="IVP39_002927"
     CDS             2045..2653
                     /locus_tag="IVP39_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7587950.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          814 a          635 c          829 g          748 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg ggtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgt
//
LOCUS       DACCSK010000275         3026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.194, whole genome shotgun sequence.
ACCESSION   DACCSK010000275 DACCSK010000000
VERSION     DACCSK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.194"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            47..1018
                     /locus_tag="IVP39_002928"
     CDS             47..1018
                     /locus_tag="IVP39_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7587951.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="IVP39_002929"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="IVP39_002929"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7587952.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="IVP39_002930"
     CDS             2045..2653
                     /locus_tag="IVP39_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7587953.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          814 a          635 c          828 g          749 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgt
//
LOCUS       DACCSK010000276         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.197, whole genome shotgun sequence.
ACCESSION   DACCSK010000276 DACCSK010000000
VERSION     DACCSK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.197"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..111
                     /locus_tag="IVP39_002931"
     CDS             <1..111
                     /locus_tag="IVP39_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7587954.1"
                     /translation="ETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            126..995
                     /locus_tag="IVP39_002932"
     CDS             126..995
                     /locus_tag="IVP39_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7587955.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            976..1710
                     /locus_tag="IVP39_002933"
     CDS             976..1710
                     /locus_tag="IVP39_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7587956.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1743..2651
                     /locus_tag="IVP39_002934"
     CDS             1743..2651
                     /locus_tag="IVP39_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7587957.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2648..2896
                     /locus_tag="IVP39_002935"
     CDS             2648..2896
                     /locus_tag="IVP39_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP7587958.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1016 a          408 c          650 g          844 t
ORIGIN      
        1 gaaacagtaa ccttggatga gttgaaggcc attgctgatg tgctaggtgt caagtttgtg
       61 caggcattta ttttaccaga tggagatgaa ataaaaatat ctaacgaata aaggaggatg
      121 tttatatggt aggtaatgtt attcaaatag taacagagaa attatcatct ttgcctttta
      181 tagaaggcat tgtattaggg ggctcacgtg caagaagcac ccatacagaa aattctgata
      241 tagatatcgg aatctattac aattcagact catttgacct gacagcgatt aatcaaattg
      301 ctacagagtt ggatgatgag aatagaaaca accttgttgt acctcccgga gcgtggggtg
      361 attgggttaa tggcggtgga tggttagtta taaacgggtg tcatgttgac ttgatattac
      421 gtgatataaa acgggtggaa caaataatca aagatacgga gcaaggaatt gttactgcca
      481 attatcagac cgggcatccc catggttata taagtgctat gtatcgtgga gaattagcga
      541 ttagtaaaat actatatgct aagaacgaaa gcttatgcga attaaaaaag caggcagaaa
      601 tttaccctac tgctctaaag aaaagcttga tgaacttttt tatatttgaa gctgaattct
      661 ctttaatgtt tgtaaaagca aatgcggggg cagaggataa atattatatt gcaggccatg
      721 tttttcgtat tatttcatgc ttaaatcaag tactatttgc atgtaataat gcttattgca
      781 ttaacgaaaa gaaagctata aaactgcttg aaacttttga atataaacct gaaaaatatg
      841 ccgaaagagt taatcatatt tttgaagtac tcggtctttc tctttttgaa tgctacgaca
      901 tgaccgagaa actttataaa gaagtgaatg aaattgtatc ggagataaat aactttttaa
      961 acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac
     1021 agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa
     1081 aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg
     1141 cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat
     1201 aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt
     1261 gccatagaag atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg
     1321 tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct
     1381 ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac
     1441 tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag
     1501 ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc
     1561 acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag
     1621 ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg
     1681 ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt
     1741 aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac
     1801 gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat
     1861 ttcaggatta tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg
     1921 actggcttaa tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat
     1981 tcccacctga agaaaaggga ttttcctatc ttatgctatt tgatgattac aataaaattg
     2041 atcttacctt attgcccttg gaagagttag ataattacct aaagggcgat aaattaataa
     2101 aggttctaat tgataaagat tgtagaatta aaagggacat agttccgact gatatagatt
     2161 atcatgtaag aaagccaagc gcaagggagt atgatgattg ctgcaatgaa ttttggaatg
     2221 taacacctta tgttattaaa ggattgtgcc gtaaggaaat tttatttgct attgatcatt
     2281 ttaatcagat tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa
     2341 caggctttaa attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg
     2401 atttgtggga gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag
     2461 cattatttct atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc
     2521 attatgccta tccggagtat gataggaata taacaaaata taccagggac atgtataaaa
     2581 aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc
     2641 gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga
     2701 accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac
     2761 agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga
     2821 tgaggaggca gattgccttg aatatattga caatactgat aagataatat atcttttaac
     2881 ccgtggtgct agttaatttt aatatcctaa agaatatc
//
LOCUS       DACCSK010000277         1970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.213, whole genome shotgun sequence.
ACCESSION   DACCSK010000277 DACCSK010000000
VERSION     DACCSK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.213"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            47..439
                     /locus_tag="IVP39_002936"
     CDS             47..439
                     /locus_tag="IVP39_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7587959.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="IVP39_002937"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="IVP39_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7587960.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          819 a          149 c          337 g          665 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaattc aaaatctttc agagaagctg ttaaaggaat
//
LOCUS       DACCSK010000278         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.236, whole genome shotgun sequence.
ACCESSION   DACCSK010000278 DACCSK010000000
VERSION     DACCSK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.236"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IVP39_002938"
                     /pseudo
     CDS             46..297
                     /locus_tag="IVP39_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IVP39_002939"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IVP39_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7587961.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACCSK010000279          902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.252, whole genome shotgun sequence.
ACCESSION   DACCSK010000279 DACCSK010000000
VERSION     DACCSK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.252"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            289..837
                     /gene="aac(6')"
                     /locus_tag="IVP39_002940"
     CDS             289..837
                     /gene="aac(6')"
                     /locus_tag="IVP39_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7587962.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          323 a          129 c          187 g          263 t
ORIGIN      
        1 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
       61 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
      121 gatctttcct attttgaata aagtgtttct gtataatttt tttattttat aagtaagata
      181 aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg
      241 ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt
      301 gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact
      361 tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa
      421 cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct
      481 caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag
      541 aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga
      601 atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac
      661 ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga acatccgtat
      721 gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg
      781 gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa
      841 aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac
      901 tt
//
LOCUS       DACCSK010000280          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.257, whole genome shotgun sequence.
ACCESSION   DACCSK010000280 DACCSK010000000
VERSION     DACCSK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.257"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            3..740
                     /gene="erm(B)"
                     /locus_tag="IVP39_002941"
     CDS             3..740
                     /gene="erm(B)"
                     /locus_tag="IVP39_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7587963.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          297 a          138 c          125 g          233 t
ORIGIN      
        1 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
       61 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
      121 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
      181 acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa
      241 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
      301 atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccgtgcgt
      361 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
      421 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
      481 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
      541 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
      601 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
      661 taaacaattt aagtaccatt acttatgagc aagtattgtc tatttttaat agttatctat
      721 tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt
      781 actaaaggga atg
//
LOCUS       DACCSK010000281          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_131
            SAMN12916585-rid10366543.guided.258, whole genome shotgun sequence.
ACCESSION   DACCSK010000281 DACCSK010000000
VERSION     DACCSK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916585
            Sequence Read Archive: SRR10233547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820102.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820102.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:28:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,920
            Genes (coding)                    :: 2,853
            CDSs (with protein)               :: 2,853
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 14
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 29 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916585-rid10366543.guided.258"
                     /isolate="RPH_EFM_131"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            3..740
                     /gene="erm(B)"
                     /locus_tag="IVP39_002942"
     CDS             3..740
                     /gene="erm(B)"
                     /locus_tag="IVP39_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7587964.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          296 a          138 c          126 g          233 t
ORIGIN      
        1 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
       61 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
      121 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
      181 acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa
      241 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
      301 atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccgtgcgt
      361 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
      421 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
      481 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
      541 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
      601 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
      661 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat
      721 tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt
      781 actaaaggga atg
//
